LOCUS       DASTPD010000001         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1224694, whole genome shotgun sequence.
ACCESSION   DASTPD010000001 DASTPD010000000
VERSION     DASTPD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224694"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="rho"
                     /locus_tag="VFR57_00005"
     CDS             complement(<1..875)
                     /gene="rho"
                     /locus_tag="VFR57_00005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU0257791.1"
                     /translation="MHLSELKTNHVSQLVEMAASNGIENASRMRKQDLIFALLKNQAK
                     KGESIYGDGTLEVLPDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEPPEAAKNKILFENLTPLHPDEAFKLERDIKAEENITGR
                     IIDIIAPIGKGQRGLIVSPPKAGKTVMLQHIAHAIIANHPDVVLIVLLIDERPEEVTE
                     MSRSVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHRKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDA"
     gene            complement(1091..1426)
                     /gene="trxA"
                     /locus_tag="VFR57_00010"
     CDS             complement(1091..1426)
                     /gene="trxA"
                     /locus_tag="VFR57_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEU0257792.1"
                     /translation="MASTTSSIQRVTDDTFEPEVLKADTPVLVDYWAEWCGPCKSIAP
                     ILDEVAKEYEGRLKIAKINVDENLLTPAKFGIRGIPTLMLFKNGNVEATRVGALSKSQ
                     LTAFLDSNI"
     gene            1514..1837
                     /gene="fdxA"
                     /locus_tag="VFR57_00015"
     CDS             1514..1837
                     /gene="fdxA"
                     /locus_tag="VFR57_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEU0257793.1"
                     /translation="MTYVVTEACIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPDSQREFTALNAELSKIWKPIIERKPAPPDADQWAKAKEKK
                     NLLER"
     gene            2016..2258
                     /locus_tag="VFR57_00020"
     CDS             2016..2258
                     /locus_tag="VFR57_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0257794.1"
                     /translation="MDQGRLAFTAVYLKSNHGYIGFIEELPGVNSHGRTLDEARETLQ
                     KLAAVVFDEERREAEELIAGKDVVRESFFVPLPRAN"
     gene            2373..3896
                     /locus_tag="VFR57_00025"
     CDS             2373..3896
                     /locus_tag="VFR57_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0257795.1"
                     /translation="MAARNWRTEDWVAVYLGFFIIVLILAAYSGKWFDFGQLRPTFRW
                     TADAQIASAAPGWRAALDNIAADADTKGRKDLAAAANGVKSALDKGDRAALEKAAGAL
                     AKVGGRNTVPGTLGSEIRGHASSSTGKVFAGQNLLKVLYIGIAGLVIGAVGFALIGGS
                     VGAFMIGFPVVFVLAWLARVIAGNGFFVDYGVEYVIFALLMGLLISNTVGTPKWLMPA
                     VQTEYFIKTGLVILGAGLLFLEVIQAGVLGILQAVLVVVVIWYICFWMARKLRVDDDF
                     AAMLSSSVAICGVSAAIATCGAIQGDKKKLSYVTSLVLIVAVPMMIIMPWIAKAFHFP
                     DMVAGAWLGGTLDTSASVVAAGALISDVAMKTGVIVKFSQNALIGVAAFILVVWWTYK
                     AGAAAGGQQPSAGIIWERFPKFVLGFLVASALFSFFLPAPMVEGTRSVLGELRTWWFA
                     LAFVCIGLETKFVDLATMEGGRPAVAFLGAQAVNIVWTLLLAYLLFGGVIFPLPDIK"
     gene            3904..4677
                     /locus_tag="VFR57_00030"
     CDS             3904..4677
                     /locus_tag="VFR57_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEU0257796.1"
                     /translation="MVWELERHGCRSTLIGAAHFFPRHFRRSLRRLLGEARIVLLEGP
                     LDEEATRKVLAAGRGSGGSMLYEAAGEKINAQLGIVRPPLDLHQLLKDLVFGRQDEWL
                     GEEMRALKPWAAFFGVWTRYRAREGWTHSLDLDAARIAAALGKQTLYLETIEEQIAAL
                     EAVPLERILDFMNVDWSAYCAAYERHYLAGELDALVAAAQAFPTYCEPIIERRDPLLA
                     ERMARELERGQACVVIGVAHCPGVLARLKELGYVNLPCP"
     gene            complement(4653..5636)
                     /locus_tag="VFR57_00035"
     CDS             complement(4653..5636)
                     /locus_tag="VFR57_00035"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0257797.1"
                     /translation="MIDLPLVFTVLMGVAILAYVVLDGYDLGVGMLMPAASRDEQNRM
                     VASIGPFWDANETWLVLGIGLLLVAFPLAHGEILGALYLPVAAMLVGLMLRGVAFELR
                     IKAEGWHRELWNWLFWAGSALASLAQGFMLGRYITGFAPGFGYWLFALIVAAGLCGGY
                     VLLGATWLVLRTDGPLQAKATAWARWGLVWVALGVALVSLATPLVSETVRDKWFDFPR
                     TAALMVLPGGTAAIWLWIWANLGKSEWKPFAGAVAIYILAFIGLAYSLFPFVLIDRLT
                     IWEAAAHPSALVFVLVGTLIVLPFIIGYTIYAYRVFRGKARADDYGHGKFT"
     gene            complement(5633..>6100)
                     /locus_tag="VFR57_00040"
     CDS             complement(5633..>6100)
                     /locus_tag="VFR57_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU0257798.1"
                     /translation="NDYAIEIPMALSVILTHDPDGVVQGLEEFGDDYPPVAPVFWSFR
                     IMVGIGVLMLALSWTGLFFLARKRQTPRWLLWAFAAFTFSGWIATLAGWMVTEIGRQP
                     WLVTGILRTAEAVGEASGAAVGASLTAYALTYAALLLSYMVVLTHLAGKGGTS"
BASE COUNT         1051 a         1906 c         2045 g         1098 t
ORIGIN      
        1 ttggcatcga cccctccggt cagcaccttg ccggaagccg ggacgacggt gttgtatgcg
       61 cgtgcaaggc gcgtgatgga atcgagcagg atgacgacgt ccttgcggtg ctcgaccagg
      121 cgcttcgcct tctcgatcac catctcggcg acctgcacgt ggcgcgaggc gggctcgtcg
      181 aaggtcgagg ccaccacctc gccgcgcacc gagcgcgaca tctcggtcac ttcctcgggc
      241 cgctcgtcga tcagcagcac gatcagcacg acgtcggggt ggttggcgat gatcgcgtgc
      301 gcgatgtgct gcagcatcac ggtcttgccg gccttcggcg ggctgacgat cagtccgcgc
      361 tggcccttgc cgatcggcgc gatgatgtcg atgatccggc cggtgatgtt ctcctcggcc
      421 ttgatgtcgc gctcgagctt gaacgcctcg tccgggtgca gcggcgtcag gttctcgaac
      481 aggatcttgt tcttggccgc ctcggggggc tcgctgttga ccttgtcgac cttgaccagc
      541 gcgaagtagc gctcgccgtc cttgggcgtg cggatctcgc cctcgatcga gtcgccggtg
      601 tgcaggttga agcgccggat ctgcgagggc gagacgtaga tgtcgtcggt gctcgcgagg
      661 taggaggtat cgggcgagcg caggaagccg aagccgtcgg gcagcacttc gagcgtgccg
      721 tcgccgtaga tcgactcgcc tttcttcgcc tggttcttga gcagcgcgaa gatcaggtcc
      781 tgcttgcgca tgcggctcgc gttttcgatg ccgttggagg ccgccatctc gacgagctgg
      841 gagacgtgat tcgtttttag ttcggaaaga tgcataggga tagggatcgg cgaagaagac
      901 gcggcgcccg cgggcgcctg cgactcaggg cgaggggatg atgaacggac tgcttccggc
      961 tgaactgcct ggggtctgct actcattctc gggaagtgcg ccccggattt tgtcttgggc
     1021 gctggacaac ctgtttgaat caggccccgc tacaggcggg gcctacctac ggcaacaaag
     1081 cctaagagct tcagatgttg ctgtcaagaa acgcggtgag ctgggacttg gacagcgcgc
     1141 cgacgcgggt cgcctcgacg ttgccgttct tgaagagcat cagcgtcggg atgccgcgaa
     1201 tgccaaattt cgccggcgtg agcaggttct cgtcgacgtt gatcttcgcg atcttcaggc
     1261 ggccctcgta ctccttcgcc acttcgtcga ggatgggcgc gatcgacttg cacgggccgc
     1321 accactcggc ccagtagtcg acgagcaccg gggtgtcggc tttcagcacc tcgggctcga
     1381 atgtgtcgtc ggtcacgcgc tggatggaac tggtggtgct cgccatgggt cctcgggaat
     1441 tggtacgggg gaaggactct attatggggt cgccccggac agttcccaag tggatggtaa
     1501 actgttgatt ctaatgacgt acgtagtcac cgaggcgtgc atcaagtgca agtacaccga
     1561 ctgcgtggac gtgtgccctg tcgactgctt tcgcgagggg cccaacatgc tggtcatcga
     1621 ccccgacgag tgcatcgact gcacgctgtg cgttcccgag tgcccggtgg aagcgatctt
     1681 tgccgaagac gacgtgccgg actcgcaaag agagttcacc gcactgaacg ccgagctctc
     1741 gaagatctgg aagccgatca tcgagcgcaa gcctgcgccg ccggatgcag accagtgggc
     1801 gaaagcgaaa gagaaaaaaa atctgctcga gcgatgagct cgcacgcagt gcagcaatcg
     1861 cactgcgaga gattgcgcat cgcaaaaaat ttgttgtcgt cgtaacgacc acatgaattt
     1921 tgaagtatca ctttcgcgaa gtttctttgc gctaagtcat acttttgaaa gcttgatcgc
     1981 gtaacctgtc ttctgctcag gcaggaggtt tacgaatgga tcaaggaagg ctcgccttta
     2041 ccgctgtgta cttgaagtcg aatcacggct acatcggctt catcgaggaa ttgcccggcg
     2101 tgaattcgca cggccgcacg ctcgacgaag cgcgcgagac gctgcagaag ctcgccgcgg
     2161 tggtattcga tgaggagcgc cgcgaagccg aggagctgat cgcgggcaaa gatgtggtgc
     2221 gcgaatcgtt cttcgtgccg cttccgcgcg cgaactaagc ttattcccga tcggtgaaag
     2281 ggcgcctgcg ggcgctcttt tcgtttgcgg cgtaggatag gcatgcaagg agggaccaag
     2341 gagggaaact cactaaaggg agggagcaac gcatggctgc caggaactgg cgcaccgaag
     2401 actgggttgc cgtctatctg ggtttcttca tcatcgttct catcctcgcc gcgtatagcg
     2461 gcaagtggtt cgacttcggg caactgcgac cgaccttccg ctggaccgcg gacgcgcaga
     2521 tcgcgtctgc ggcgcccggc tggcgcgcgg cgctcgacaa cattgccgcc gacgccgaca
     2581 ccaagggcag gaaagacctc gccgccgccg cgaacggcgt gaagagcgcg ctcgacaagg
     2641 gcgaccgcgc ggcgctcgag aaggcggccg gcgcgctggc caaggtcggc ggccgcaaca
     2701 ccgtgccggg cacgcttggc tcggagatcc ggggccacgc atcgtcgagc accggcaagg
     2761 tattcgccgg gcagaacctg ctcaaggtgc tctatatcgg catcgccggg ctggtaatcg
     2821 gcgcggtcgg ctttgcgctg atcggcggca gcgtcggcgc gttcatgatc ggcttcccgg
     2881 tggtgttcgt cctcgcctgg ctcgcgcgcg tgatcgccgg caacgggttt ttcgtggact
     2941 acggcgtgga gtacgtcatc ttcgcgctgc tgatggggct cctgatcagc aacaccgtcg
     3001 gcacgccaaa atggctcatg cccgcggtgc agaccgaata cttcatcaag accggcctcg
     3061 tcatcctcgg cgcgggcctg ctcttcctgg aggtgattca ggccggcgtg cttggcatcc
     3121 tgcaggcggt gctcgtggtg gtggtgatct ggtacatctg cttctggatg gcgcggaagc
     3181 tccgcgtcga cgacgacttc gccgccatgc tctcctcgtc ggtggcgatc tgcggcgtgt
     3241 ccgcggcgat cgcgacctgc ggggcgatcc agggcgacaa gaagaagctg tcctatgtca
     3301 cctcgctcgt gctgatcgtc gccgtgccga tgatgatcat catgccgtgg atcgcgaagg
     3361 cgttccactt tcccgacatg gtggccggtg cttggctcgg cggcacgctc gacaccagcg
     3421 cctcggtggt cgccgcgggc gcgctgatca gcgacgttgc gatgaagacg ggtgttatcg
     3481 tcaagttctc gcagaacgcg ctcatcggcg tcgccgcgtt catcctcgtg gtctggtgga
     3541 cgtacaaggc aggtgccgcc gcaggcggcc agcagccgag cgcgggcatc atctgggagc
     3601 gcttcccgaa gttcgtgctc ggcttcctgg tcgcttccgc gctgttctcc ttcttcctgc
     3661 cggcgccgat ggtggagggc acgcgctcgg tgctcgggga gctgcgtacc tggtggttcg
     3721 cgcttgcttt cgtgtgcatc ggcctggaga ccaagttcgt cgacctcgcg accatggaag
     3781 gcgggcgccc agcggtagcg ttcctcggtg cgcaggcggt caacatcgtg tggacgctgc
     3841 tgcttgccta tctgctgttc ggcggcgtca tcttcccgct acccgatatc aagtgacctg
     3901 aggatggtgt gggagctcga gcggcatggc tgccgctcga ctctcatcgg ggcggcgcat
     3961 ttcttcccgc gccattttcg ccgcagcctg cgccggctgc tcggcgaagc gcgcatcgtc
     4021 ctgctcgaag ggcctctcga cgaggaggcc acgcgcaagg tgctcgccgc cgggcgcgga
     4081 tccggcggct ccatgttgta cgaagctgca ggagaaaaga tcaatgcgca gctcggcatc
     4141 gtgcgcccgc cgctcgactt gcaccaattg cttaaggacc tcgtcttcgg gcgacaggac
     4201 gagtggctgg gagaggaaat gcgcgcgctc aagccctggg ccgcgttctt tggcgtctgg
     4261 acgcgttacc gcgcgcgcga gggctggact cattcgctcg atctcgacgc ggcgcgcatc
     4321 gccgccgcgc tcggcaagca gaccctctac ctcgagacca tcgaggagca gattgccgcg
     4381 ctcgaagccg tgccgctcga gcggatactg gacttcatga acgtagattg gtccgcgtac
     4441 tgcgcggcgt atgaacggca ttacctcgcc ggcgagctgg acgcgctcgt ggccgccgcg
     4501 caggcgtttc ccacctactg cgagccgatc atcgagcggc gcgatccgct gcttgctgag
     4561 cgcatggcgc gggaactcga gcgcggccag gcatgcgtgg tcataggcgt cgcgcactgc
     4621 cccggcgtgc tcgcgcggct gaaggagctc ggctacgtga acttgccgtg cccgtagtcg
     4681 tcggcgcgcg ccttgccgcg gaacacgcgg taggcataga tcgtgtagcc gatgatgaat
     4741 ggcagcacga tgagcgtccc caccagcacg aagacgaggg cgctcgggtg cgccgccgcc
     4801 tcccagatcg tcaggcgatc gatcagcacg aacgggaaca ggctgtaggc gaggccgatg
     4861 aacgcgagga tgtagatcgc caccgcgccg gcgaacggct tccactcgga tttgccgaga
     4921 ttcgcccata tccacagcca gatcgccgcc gttccgccgg gaagcaccat gagcgcggcc
     4981 gtgcgcggga aatcgaacca cttgtcccgc accgtctcgc ttacgagcgg ggtggcaagg
     5041 ctgacgagcg cgacgccaag cgcgacccaa acgaggcccc agcgcgccca cgccgtcgcc
     5101 ttggcctgca gcgggccgtc ggtgcgcagg acgagccagg tggcgccgag gagaacgtag
     5161 cccccgcaca ggccggcggc gacgatgagc gcgaagagcc aataaccgaa gccgggggca
     5221 aagccggtga tgtagcgccc gagcatgaag ccctgcgcca ggctggcgag cgcggagccc
     5281 gcccagaaca gccagttcca cagttcccgg tgccagccct ccgccttgat gcgcagctcg
     5341 aaagcgacgc cgcgcagcat gagcccgacc agcatcgccg caaccggcag gtagagcgct
     5401 cccaggatct ctccgtgtgc gagcgggaag gcgacgagga gcagcccgat cccgagcacg
     5461 agccaggtct cgttcgcgtc ccagaagggg ccgatcgagg ccaccatgcg gttctgctcg
     5521 tcccggcttg cggccggcat cagcatgccg acgccgaggt cgtagccgtc gagcaccaca
     5581 taggcgagga tcgccacgcc catgaggacc gtgaacacca gcggcaggtc gatcacgacg
     5641 tccccccttt ccctgcgagg tgggtgagga ccaccatgta cgacagaagc agcgcggcat
     5701 aggtcagcgc gtaggcggtc aagctcgcgc cgaccgcggc gccgctcgcc tcgcccaccg
     5761 cttccgcggt gcgcaggatc ccggtgacga gccagggctg ccggccgatc tcggtcacca
     5821 tccagccggc gagcgtcgcg atccagcccg agaaggtgaa cgcggcgaac gcccagagca
     5881 gccaccgcgg cgtctgtctt ttccgggcca ggaagaaaag accggtccag gaaagcgcga
     5941 gcatcagcac gccgatgccc accatgatgc ggaaagacca gaacaccggc gcgaccggcg
     6001 ggtagtcgtc cccgaactcc tccagtccct gcaccacgcc atccgggtcg tgtgtgagga
     6061 tgaccgacag ggccatggga atctcgatgg cgtagtcgtt
//
LOCUS       DASTPD010000002         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1428789, whole genome shotgun sequence.
ACCESSION   DASTPD010000002 DASTPD010000000
VERSION     DASTPD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428789"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFR57_00045"
     CDS             complement(<1..578)
                     /locus_tag="VFR57_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0257799.1"
                     /translation="MREFSRIKRLPPYVFNITNELKMAARRRGDDIIDLSMGNPDGPT
                     PKHIVAKLVEASQRIDTHGYSVSRGIPRLRKAIADWYRRRYAVDLDPDAEAIVTIGSK
                     EGLAHLMLACLERGDTVLVPNPSDPIHIYGAIIAGADIRSVRMTPGVDFFAELERAIR
                     ELMPKPKMLVIGFPSNPTAQCVDLSFFEKIIAL"
     gene            593..961
                     /locus_tag="VFR57_00050"
     CDS             593..961
                     /locus_tag="VFR57_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEU0257800.1"
                     /translation="MKLQASRPSGVNTITGYGEGYVMVNGERHASSLVVLADRVEDWS
                     AERFDALTAEDFGFLKELGVEIVLLGTGPRQRFPHPRLTAALAQAGIGLEVMDVQAAC
                     RTYNILVAEERRVAAALLFE"
     gene            958..1851
                     /locus_tag="VFR57_00055"
     CDS             958..1851
                     /locus_tag="VFR57_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0257801.1"
                     /translation="MSLFPGFIRKRIRTSAATINLVAGGEGPPLLLLHGYPQTHAIWH
                     KVAPGLARDYTVVCADLRGYGDSSKPRGVPGHANYSKRAMALDMIEAMEALGFIKFHV
                     VGHDRGGRVAHRMARDHARRVQTLTVLDISPTLKMYESTNMQFAKAYYHWFFLIQEAP
                     LPERMLKSMGPDYILKRLGRGKSGLKVFDRRALAEYARCFDPHSTCEDYRAAATIDLM
                     HDRKDLHTKLKMPVLALWGRQGVIEALFDCLADWREVALDVRGRALQCGHFIPEEKPK
                     ELLAELRRFLRGEKPLPIRTP"
     gene            2221..>2762
                     /locus_tag="VFR57_00060"
     CDS             2221..>2762
                     /locus_tag="VFR57_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0257802.1"
                     /translation="MKPSRKQAKPRKLFVIDTNVLMHDPTSLFRFDEHDLYLPIATLE
                     ELDQHKRGLSDVSRNARQASRFLDEIVSGDVESINKGLALKIRDGQQSKGRLFLQTEA
                     INGELPATLASGRTDNQILAVVRALQQKQPEREVVLVSKDINMRIKARALGLAAEDYT
                     NDKVLEDADLLYTGVRALPR"
BASE COUNT          524 a          895 c          875 g          468 t
ORIGIN      
        1 agcgcaatga tcttctcaaa gaaactcaga tcgacacatt gcgcggtcgg attcgacggg
       61 aacccgatca ccagcatctt cggcttcggc atgagctcgc ggatcgcgcg ctcgagctcg
      121 gcgaagaaat ccacgccggg cgtcatgcgc accgagcgga tgtcggcgcc cgcgatgatc
      181 gcgccgtaga tgtggatggg gtccgacggg ttgggcacga gcaccgtgtc gccgcgctcg
      241 aggcacgcaa gcatgagatg cgcgaggcct tccttcgagc cgatggtgac gatcgcctcc
      301 gcgtcgggat ccaggtcaac cgcgtagcgg cgccgatacc agtctgcgat cgcttttctc
      361 agcctcggga tgccgcgcga gaccgagtag ccgtgcgtgt cgattcgttg cgacgcctcg
      421 accagctttg cgacgatgtg cttcggcgtc gggccgtcgg ggttccccat cgacagatcg
      481 atgatgtcgt cgccgcgccg ccgcgccgcc atcttgagct cgttcgtgat gttgaagacg
      541 tacggcggca gccgcttaat ccttgaaaac tcacgcattg tagaattcta gcgtgaaact
      601 ccaggcctcc cgtccgagcg gcgtgaacac gatcactgga tacggcgaag gctacgtgat
      661 ggtgaacggc gagcggcacg cttcgagcct ggtggtgctc gccgaccgcg tcgaggattg
      721 gtcggccgag cgtttcgacg cgctcactgc ggaggatttc gggtttctga aggagctcgg
      781 cgtcgagatc gtgctgctcg gcaccggtcc gcgtcagcgc tttccgcatc cacggctcac
      841 cgccgcgctc gcgcaggcgg ggatcggact cgaggtgatg gacgtgcagg ccgcctgccg
      901 cacttacaac atcctcgtcg ccgaggaacg ccgagttgcg gccgcgctct tgttcgaatg
      961 agccttttcc cgggcttcat caggaagaga atcaggacga gcgcagcgac cattaacctc
     1021 gtcgccggcg gcgaaggccc gccgctactg ctgctccacg gctacccgca gacgcatgcc
     1081 atctggcaca aggtggcgcc ggggctcgcg cgcgactaca ccgtggtgtg cgccgacctg
     1141 cgcggctacg gcgactcgtc gaagccgcgc ggcgttcccg gccacgcgaa ttactcgaag
     1201 cgcgcaatgg cgctcgacat gatcgaggcg atggaagccc ttggcttcat caagttccac
     1261 gtcgtcggcc acgaccgcgg cggccgcgtc gcgcatcgca tggcgcgcga ccacgcccgg
     1321 cgcgtgcaga cgctgaccgt gctcgacatc tcgcccacgc tcaagatgta cgagagcacc
     1381 aacatgcagt tcgcgaaggc ctactaccac tggttcttcc tgatccagga ggcgccgctg
     1441 cccgagcgga tgctgaagag catgggaccc gactacatcc tcaagcgcct cggcaggggg
     1501 aagagcggcc tcaaggtgtt cgaccggcgc gcgctcgccg agtacgcgcg ctgctttgat
     1561 ccacactcca cctgcgagga ctaccgcgcg gccgccacca tcgacctcat gcacgacagg
     1621 aaggacttgc atacgaagct caagatgccg gtactcgcgc tctggggccg gcagggcgtg
     1681 atcgaggcgc tctttgactg ccttgccgac tggcgggaag tcgcgctcga tgtccgcggc
     1741 cgcgcgctcc agtgcggaca tttcatcccc gaggaaaagc cgaaggagct cctcgcggaa
     1801 ctgcgccggt tcttgcgtgg cgaaaaaccc ttaccaatca ggaccccgta gcgtccgctt
     1861 gcgctggaaa cattagttgt gttcaatgaa agcgctacag cgcattgcta agaagggtaa
     1921 ttagaacctc tggaaatgct cggcaagccg gttgcggact gcagccttcc cagcaccggc
     1981 ggcggcacct tcaggctctc gagcgctcgc acgagcaagc tggtccagta tttctatcca
     2041 aaggacaaca cgcttcggcg tttcgtgcga ctcgctcaag tcgcaggagg cattcaaggc
     2101 gaagatgaag ttcccgagaa agatccgcgg catcgagcgc ccacgtccag gaggtattga
     2161 actttgttaa agccccatga tgtcgagcca cccgcagtag cgttagagtc agtccccgcc
     2221 gtgaagccgt cccgcaagca agcaaagcca agaaagctct tcgtcatcga cacgaacgtg
     2281 ctgatgcacg atccgacgag cctcttccgc ttcgacgagc acgacctcta cctgccgatc
     2341 gcgaccctgg aagagctcga ccagcacaag cgcgggctgt cggacgtctc gcgcaacgcg
     2401 cgccaggcga gccgcttcct cgacgagatc gtgagcgggg atgtcgagtc gatcaacaag
     2461 gggcttgcgc tcaagatcag ggacgggcag cagtccaagg ggcggctttt cctgcagacc
     2521 gaagcgatca acggcgagct gccggcgacg ctcgcctcgg gccgcaccga caaccagatc
     2581 ctcgccgtgg tgcgtgcgct gcagcagaag cagcccgagc gcgaggtggt gctggtgtcg
     2641 aaggacatca acatgcgcat caaggcgcgc gcgctcgggc tcgccgccga ggattacacc
     2701 aacgacaagg tgctcgagga cgccgacctg ctctacaccg gcgtgcgcgc gctgccgcgc
     2761 ga
//
LOCUS       DASTPD010000003         6586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1224841, whole genome shotgun sequence.
ACCESSION   DASTPD010000003 DASTPD010000000
VERSION     DASTPD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224841"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFR57_00065"
     CDS             <1..383
                     /locus_tag="VFR57_00065"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0257803.1"
                     /translation="YIGPRVFQFQRSRWFNPAHLRRASEFYERHGGKTIILARFVPIV
                     RTYVPFVAGVGAMPYGRYLGFCVLGALMWVVSLCLLGFWFGNQPIVKNNLTAVILIIV
                     AISVSPGFFAWLRSRREARAAPRA"
     gene            complement(387..1550)
                     /locus_tag="VFR57_00070"
     CDS             complement(387..1550)
                     /locus_tag="VFR57_00070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0257804.1"
                     /translation="MALLAALSVSAVEAQVLSTRIWPAPEYTRLTFESKEEIQYKIFS
                     VTHPARLVLELQTNDITVALAELNGKVTGEDPYIQGLRVARNRPGVVRVVLDLKQDVR
                     PQIFTLRPVADYGHRLVLDIHPIVPHDVMAQLLEETEKLRPRLRPGAPRLATIVIDPG
                     HGGEDPGAIGRRGSREKDVTLSIARRLKKLIDGEPNMRALLTRDGDYFLALGARVEKA
                     RAVKADLLVSIHADAFTRPNVRGSSVFALSDKRATSELARALAKKENESDLIGGVALQ
                     TKDDHVRRTILDLSQSATIDHSLRLGSSVLKQLGGVNDLHKPRVEQASFAVLTAPDVP
                     SILVETAFISNPEEERRLNDDVYQDKLAQAILEGIREYIAKHPPRPVGPIATR"
     gene            complement(1523..1957)
                     /gene="tsaE"
                     /locus_tag="VFR57_00075"
     CDS             complement(1523..1957)
                     /gene="tsaE"
                     /locus_tag="VFR57_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU0257805.1"
                     /translation="MDATLRLGEALAAGARPGRVLFISGDLGAGKTTLVRGLLRGLGH
                     PGRAKSPTYALVEPYSFPSLDLYHFDFYRFKDRSEWLSSGFREHFNAQSLCVVEWPEK
                     VGDLLAPPDLHIRLDFDGDARSASLEARSGAGEAWLSSLRFP"
     gene            1890..2999
                     /gene="queG"
                     /locus_tag="VFR57_00080"
     CDS             1890..2999
                     /gene="queG"
                     /locus_tag="VFR57_00080"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0257806.1"
                     /translation="MNSTRPGRAPAASASPRRSVASTSASLNERIKAWGRELGFQAVG
                     IADADLSAAEPRLLEWLARGWHGEMEYMARHGALRARPAELKPGTLRVISCRMDYLGD
                     APQGDLPRADVAFVARYARGRDYHKVLRDRLQKLCDRIREEVGDFGYRVFTDSAPVME
                     VELAARAGLGWRGKHTLLLSRDAGSWFFLGEVYCDIPLAVDVPETNHCGTCARCIDVC
                     PTQAIRAPYELDARRCISYLTIEHKSAIPEELRPLIGNRVYGCDDCQLVCPWNRFARL
                     SPEGDFRTRNGLDRATLLELFAWTEEEFDERLRGSPIRRIGYERWLRNLAVGLGNAPT
                     SLHVVAALRARAEHPSALVREHVRWALSRHASVAT"
     gene            2980..3876
                     /locus_tag="VFR57_00085"
     CDS             2980..3876
                     /locus_tag="VFR57_00085"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0257807.1"
                     /translation="MQAWQPDHFLPGFEALELEFPDDYDGRVLATLVRLPVRNAPRGS
                     VLWVHGFIDYFFQRHVAERFALEGYAFYALDLRKHGRSNRPHQHPNFCKNIAEYYADI
                     TRSIDVIGEPVLLAGHSTGGLITSLYAYEGPHRERVQALWLNSPFFDWKLPDWRKAQL
                     HMAAALGRFFPFLSDRKTLLPDYGRSLLDAGWQFDLALKPLEGFPVYYGWLGAIADAH
                     AKVHRGLDIRCPVLCMHSDEADIVLDWRHISRWSRSLGPDVTVLAFPGAMHDLTLSSP
                     EVREEMFRQLFAWVERAVMSPA"
     gene            complement(3827..4195)
                     /locus_tag="VFR57_00090"
     CDS             complement(3827..4195)
                     /locus_tag="VFR57_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009915688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEU0257808.1"
                     /translation="MMADLILVVHFLIVLFIVGGLLSVWIGAAVGWRWVRNPWFRYLH
                     LGAIAFVAAEALLGVMCPLTVWEDALRGGVRAETFVGRWVRYFLYYEAPEWVFTLAYV
                     AWTVATLATLRLFPPRQRAA"
     gene            4241..4762
                     /locus_tag="VFR57_00095"
     CDS             4241..4762
                     /locus_tag="VFR57_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0257809.1"
                     /translation="MNAAQRAVAAEITAGPRGEVRGPFIALLHNPELARHVQRLGEHL
                     RWHGKLPPRLKELAVLVTARRWTCQHEWVMHSKLALDGGLAPQIVEAIQAGREPEGTS
                     ADEKAVYAFCSELHATGRAGDQSFSEIENRFGLDGALELIALNGYYTLMAMVLNTAQL
                     PLPGNAEPPLTPL"
     gene            4792..5736
                     /locus_tag="VFR57_00100"
     CDS             4792..5736
                     /locus_tag="VFR57_00100"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0257810.1"
                     /translation="MGWCLLVPQTARGQQKPRRVAWLGVGRAGSPSPFLEAFRTGLRD
                     WGWVEGSNLQLEAFFTDGSPESSERVANQAIASNPELLVVYGRDVPVASRLKPSCPVV
                     FAFSGDPVDAGIVKSFSRTETNFTGVSFMSLDLAAKRIELLKELAPNTRLLAVVARPE
                     HAGEHRERAASAEVAAKLGLRMFYAPIQGPKDLEAALRSIAGQKCDALVTFPDAVTLA
                     NAGRIANFAEEARIATVSGWASFADNGFLLSYGPNLRESYRRLARFADRVLRGARPAD
                     IPVELPSVVELVLNARTARVLALSIPPALRARADRVIE"
     gene            complement(5755..>6586)
                     /locus_tag="VFR57_00105"
     CDS             complement(5755..>6586)
                     /locus_tag="VFR57_00105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0257811.1"
                     /translation="NGSTLVSTGELAANLAKWRVFDCRHDLAKPELGEQQYKEGHIPG
                     ALFAHLDRDLSGRKTGSNGRHPLPDPQDFEKWLEHTGLTPSDQAVCYDAGPGAMAARL
                     WWMLRWIGHDKVAVLDGGFAKWTKEGRPVTADVPRFTRFNYPIKLRADSAVDVATVEN
                     NLGKSLLLDARAPVRFRGEQEPIDPVAGRIKGAKNRFNMDNLSPEGVFKDSQSLRRDF
                     EAVLGRRSPDEVIHYCGSGVAACHNLLAMEIAGLKGGKLYAGSWSEWSADSARASERG
                     "
BASE COUNT         1085 a         2174 c         2144 g         1183 t
ORIGIN      
        1 gctatatcgg cccgcgcgtc ttccagttcc agcgctcccg ctggttcaat ccggcgcacc
       61 tgcggcgcgc gagcgagttc tacgagcgtc acggcggcaa gaccatcatc ctcgcgcgct
      121 tcgtgcccat cgtgcgcacc tatgtgccct tcgtcgccgg cgtcggcgcg atgccgtacg
      181 ggcgctatct cggtttctgc gtgctaggcg cgctcatgtg ggttgtgtcg ctctgcctgc
      241 tcggcttctg gttcggcaac cagccgatcg tgaagaacaa cctcacggcg gtgatcctca
      301 tcatcgtcgc tatttccgtc tcaccgggct tctttgcgtg gttgcgctcg cggcgcgagg
      361 cacgagcggc accgcgcgca taaagatcaa cgagtagcga tcgggccgac ggggcgaggt
      421 ggatgcttcg cgatgtattc gcggatgcct tcaagaatcg cttgcgccag cttgtcctga
      481 tacacgtcgt cgttcaggcg ccgctcctct tcggggttcg agatgaaggc ggtctcgacg
      541 aggatcgagg gcacgtcggg ggcggtgagg accgcgaagc tcgcctgctc gacgcgcggc
      601 ttgtgcaggt cgttcacgcc gccgagctgc ttcagcaccg agctgcccag ccgaaggctg
      661 tggtcgatgg tcgcgctctg cgagagatcg aggatcgtgc gacgcacatg gtcgtccttg
      721 gtctgcagcg ccacgccgcc gatcaggtcg gactcgttct ccttcttggc aagcgcgcgc
      781 gccagctccg aggtggcgcg cttgtcggag agcgcgaaca ccgaagaccc tcgcacgttc
      841 ggacgcgtga acgcgtcggc gtgaatcgag accaggaggt ccgccttcac ggcacgcgcc
      901 ttttccacgc gagcgccgag cgcgagaaag tagtcgccat cgcgcgtaag gagcgcccgc
      961 atgttcggct cgccgtcgat cagcttcttc aggcggcgcg cgatgcttag cgtgacgtct
     1021 ttttcgcgcg aaccgcgccg gccaatggcg cccggatctt cgccgccgtg ccccggatcg
     1081 atcacgatcg tcgcgaggcg cggcgcgccg ggtctaagcc gcgggcgaag cttttctgtt
     1141 tcctcgagca gctgcgccat gacatcgtgg gggacgatgg gatggatgtc gagcaccagc
     1201 cggtggccgt aatcggcgac gggacgcagc gtgaagatct gcggccgcac gtcctgcttc
     1261 agatcgagaa cgacgcgcac gacgccgggg cggttgcgcg cgacgcgcaa accctggatg
     1321 tacggatctt ccccggtgac cttgccgttc agctcggcaa gcgcgaccgt gatgtcgttc
     1381 gtttgcagct cgagcacgag gcgcgccgga tgcgtgacgc tgaagatctt gtactggatc
     1441 tcttccttcg attcgaaggt gaggcgcgtg tattccggcg cgggccagat gcgagtggag
     1501 aggacctgcg cctcgacggc gcttacggaa agcgcagcga ggagagccag gcctccccag
     1561 cgcctgagcg cgcttccagg ctcgcgctgc gcgcatcgcc atcgaaatcc agccggatgt
     1621 gcaggtccgg cggcgcgagc aggtcgccga ccttctcggg ccattccacg acacagagcg
     1681 actgcgcgtt gaaatgctcg cggaagcccg agcttagcca ttccgagcgg tctttgaatc
     1741 gataaaaatc aaagtgatat aagtctaagc tcggaaagga gtaaggttca actaaggcat
     1801 acgtcgggct cttggcgcgg ccggggtggc cgagcccccg tagaaggccg cgaacgagcg
     1861 tagtcttgcc cgccccgaga tcccccgaga tgaatagcac ccgtccaggc cgagctccgg
     1921 ccgcgagcgc ttctccgagg cgcagcgtcg cgtccacgtc agccagcttg aatgagcgca
     1981 ttaaggcgtg ggggcgtgag ctcggttttc aggccgtcgg catcgccgat gcggacctgt
     2041 cggcagccga gccgcggctc ctcgaatggc tcgcgcgggg ctggcatggc gagatggagt
     2101 atatggcgcg ccatggcgcc cttcgcgcgc gcccggccga gctcaaaccc ggaacgctgc
     2161 gcgtcatctc ctgtcgcatg gactacctgg gagatgcgcc gcagggcgac ctgccgcgcg
     2221 ccgacgtcgc attcgtcgcg agatacgcgc gcggccgcga ctaccacaag gtcttgagag
     2281 accgcctgca gaagctctgc gatcgcattc gcgaggaggt cggcgacttc ggttatcgcg
     2341 tcttcaccga ttcggctccg gtgatggagg tcgagctggc cgcccgcgcc ggcctgggct
     2401 ggcgcggcaa gcacacgctg ctcctcagcc gggatgccgg ttcctggttt tttctgggcg
     2461 aggtctactg cgatattccg ctcgccgtcg atgtgccgga gacaaaccat tgcggcacgt
     2521 gcgcgcgctg catcgacgtc tgcccgacgc aggcgattcg cgccccgtac gagctcgacg
     2581 cccggcgctg catctcgtac ctcacgatcg agcacaagag cgcgattccg gaggaactgc
     2641 ggccgctcat cggcaaccgc gtttatggct gcgacgattg ccagctcgtc tgcccgtgga
     2701 accgcttcgc gaggctaagc ccggaaggcg atttccgtac gcgcaatggc ctcgaccgcg
     2761 ccacgctcct cgagcttttc gcctggaccg aagaggagtt cgacgagcgt ctgcgcggct
     2821 caccgatccg gcgcatcggc tacgaacgct ggctgcgtaa cctcgcggtc gggctgggga
     2881 acgcgcccac atcgttacac gtggtggccg ctttgcgggc ccgggcggag catccctccg
     2941 cgctagtcag ggagcatgtt cgttgggcac tctcacgcca tgcaagcgtg gcaacctgac
     3001 cacttcctgc ccgggttcga ggcgctagag ctcgagttcc ccgacgacta cgacggccgt
     3061 gtcctggcga cgctcgtacg cctgcccgtg cgcaacgcgc cgcgcggctc ggtgctgtgg
     3121 gtgcacggct tcatcgacta cttcttccaa cgccatgtcg ccgaacgctt cgcgctcgaa
     3181 ggctacgcgt tctacgcgct tgacctgcgc aagcacgggc gctcaaatcg gccccatcag
     3241 catccgaatt tctgcaagaa catcgccgag tactacgccg acatcacccg ctcgatcgat
     3301 gttatcggtg agcccgtgct ccttgccggg cattccaccg gcggactgat cacgtcgctc
     3361 tacgcgtacg aaggcccgca ccgggagcgc gtgcaggcgc tgtggctgaa tagtccgttc
     3421 ttcgactgga agcttcccga ctggcgcaag gcgcaactgc acatggcggc cgccctcgga
     3481 cggttcttcc cgttcctgag cgaccggaag acgctccttc ccgactacgg ccgctcgctc
     3541 ctcgacgcgg ggtggcagtt cgacctcgcg ctcaagcccc tcgaaggctt cccggtgtac
     3601 tacggctggc tcggcgcgat cgccgatgcg cacgccaaag tgcaccgcgg cctcgacatt
     3661 cgctgcccgg tgctctgcat gcactccgat gaggccgaca ttgttctcga ctggcgccac
     3721 atctcgcgct ggtcgcgctc gctcgggccg gatgtcaccg tgctcgcgtt tcccggcgcc
     3781 atgcatgacc tgacgctctc tagtccggaa gtccgcgagg agatgttcag gcagctcttt
     3841 gcctgggtgg aaagagccgt aatgtcgcca gcgtagccac cgtccatgcg acataggcga
     3901 gcgtgaagac ccattccggc gcctcgtagt acaggaagta gcgcacccag cgccccacga
     3961 acgtttctgc acgcacgcca ccgcgcagcg cgtcttccca tacggtgagc gggcacatca
     4021 caccgagcag cgcctcggcc gcgacaaacg cgatcgcgcc gagatgcaga taacgaaacc
     4081 agggattgcg tacccagcgc catccgactg cggcgccgat ccacaccgaa aggagcccgc
     4141 cgacgatgaa gaggacgatg aggaagtgga cgacgaggat caggtcggcc atcatcgccc
     4201 aagcatggca caacgcttcc cgaagctcac gctcgaacag atgaacgccg cgcagcgcgc
     4261 agtcgccgcc gagattaccg ccggcccgcg tggcgaggta cgcggcccgt tcatcgcgct
     4321 cctccacaat cccgagctcg cgcgccatgt gcagcgactg ggtgagcatc tgcgctggca
     4381 tggcaagctc ccgcccaggc tgaaagagct tgcagtgctc gtcaccgcgc ggcgctggac
     4441 ttgccagcac gagtgggtca tgcactcgaa gctggcgctc gatggtggat tggcgccgca
     4501 gatcgtcgaa gccatccagg caggccgcga gcccgagggc acgtcggcgg atgagaaagc
     4561 cgtttacgcc ttctgcagcg aattgcacgc cacaggacgt gcaggagatc aatccttcag
     4621 cgagatagag aaccggtttg gcttggacgg cgcgctcgag ctcatcgcgc tgaacggcta
     4681 ctacacgctg atggcgatgg tactcaacac cgcccaattg ccccttccgg gcaacgccga
     4741 accgccgctg acgcccttat gacaagccaa cgccgtcagt tgttgattgc catgggttgg
     4801 tgcctactcg tgccgcagac ggcccggggt cagcagaagc cgcggcgcgt cgcttggctt
     4861 ggcgtcggac gcgccggctc gccgtcgcct ttcctggagg cgttccgcac cggactgcgt
     4921 gactggggat gggtcgaagg gagcaatctc caactcgagg cctttttcac tgacggttcg
     4981 ccggagagct cggagcgcgt ggcaaaccag gcaatcgcaa gcaatccgga gctcctcgtc
     5041 gtctatgggc gggatgtacc ggtcgccagc cgcctcaagc ccagctgtcc ggtggtattc
     5101 gccttcagcg gcgatccagt ggacgccggc atcgtgaaaa gcttttcgcg gacggaaaca
     5161 aacttcaccg gtgtcagttt catgtcgctc gatttggcag ccaagcgcat tgagctactc
     5221 aaggagctcg cgccaaacac gcgcctcctc gcagtggtag cgcgccctga gcacgccggc
     5281 gagcacaggg agcgcgccgc ctcggcggag gtggcggcca agctcggttt gcgaatgttc
     5341 tatgcaccaa tccaagggcc caaggacctc gaagctgcgc tgcgatccat tgccggccaa
     5401 aagtgcgatg cactggtcac ctttcccgac gcagtcacgc tggccaacgc gggccgtata
     5461 gcgaacttcg cggaagaggc gcgtattgcc acggtttccg gctgggcgtc cttcgccgac
     5521 aacggcttcc tcctttccta cgggcccaat ctgcgcgagt cgtatcgaag gcttgcgcgc
     5581 tttgcggacc gggtgctgcg cggtgcgcgg cccgctgaca tcccggttga gctgcccagc
     5641 gtggtggagc ttgttttgaa tgcgcgcacc gcgcgcgtcc ttgcgttgtc cattccgcca
     5701 gcgctccggg cgcgtgccga ccgagtcatc gaatgaccgc tgttggctga gcggctagcc
     5761 gcgctcactg gcccgtgcgg agtccgcgct ccactcgctc cacgagcccg cgtagagctt
     5821 tccgccttta aggcccgcga tctccatcgc gaggaggttg tggcacgcgg cgacgcccga
     5881 gccgcagtaa tggatgactt cgtcgggcga acgtctgccg aggaccgctt cgaaatcgcg
     5941 gcgcaacgat tggctgtcct tgaagacgcc ctccggcgag aggttgtcca tgttgaagcg
     6001 attcttcgcg cccttgatgc gcccggccac gggatcgatc ggttcctgct cgccgcggaa
     6061 gcgcaccggg gcgcgcgcgt cgaggagtag cgacttgccg aggttgttct ccaccgtggc
     6121 gacgtcgact gcggaatcgg cgcgcagctt gatggggtaa ttgaagcggg tgaagcgcgg
     6181 cacgtcggcc gtcactggtc ggccctcctt ggtccacttt gcgaaaccgc cgtccagtac
     6241 ggcgaccttg tcgtggccga tccagcgcag catccaccac aggcgtgccg ccatcgcgcc
     6301 ggggcctgcg tcatagcaaa ccgcctggtc cgagggtgtg agtccggtgt gctcgagcca
     6361 cttctcgaag tcctgcgggt cgggaagcgg atggcgcccg ttcgagccgg tcttgcggcc
     6421 cgaaaggtcg cggtcgagat gggcgaagag cgcgcccggt atgtggcctt ccttgtactg
     6481 ctgctcgccg agctcgggct tggcgaggtc gtggcggcag tcgaacaccc gccacttggc
     6541 caggttcgcc gctagctcac cggtcgagac gagcgtggag ccattc
//
LOCUS       DASTPD010000004         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_204792, whole genome shotgun sequence.
ACCESSION   DASTPD010000004 DASTPD010000000
VERSION     DASTPD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204792"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFR57_00110"
     CDS             complement(<1..619)
                     /locus_tag="VFR57_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEU0257812.1"
                     /translation="MSVADNPDRELAEALEAARQAHFDLHGAACDYAALARSPARERL
                     AACLVALADFDIKRAQIPSQLAFWINVFNAGVLRDARELESAAGVRMVEAFFERPRLR
                     IGEHSYSLDDIEHGLLRGNVAKPGRSQPPMKRGDARLEYMPIVFEERMHFALHSACRS
                     SPPLRAFDGGKLDRQLEEAAADYIRRNVRVERQGAVLVLPRIFRWF"
     gene            646..2244
                     /locus_tag="VFR57_00115"
     CDS             646..2244
                     /locus_tag="VFR57_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0257813.1"
                     /translation="MQNFGAFDYVIVGAGSAGCLLANRLSRNSEKKVLLLEAGGRDDW
                     FWIDIPVGYLYTIANPRTDWCYKTEPDEHLAGRSIHYARGRVLGGCSSINAMIYMRGQ
                     KEDWDYWASLGNRGWSWEDVLPLFLSLEDYEHGAVDGYGAGGELRIEDPRVRWDIIDA
                     WREAAAECGIPPVKVFNRGDNFGCAYFQMNQRRGRRWSATNAFLRPVTHRPNLTVMTG
                     ASVSKLIFENATCTGVEFVHRNEKFSVQATKETILAAGAIGSPQLLQLSGIGPAGLLE
                     KHQIKLLRHLPGVGENLHDHLQIRMQYKVKNVKTLNGIANSLWGKAAMALEYLVFRTG
                     PLTMPPSQAGAFARSDPAQPTPNIEWHVQPLSLDKFGDPLHSFPAITPSVCNLRPASR
                     GWVRIKSADPAVYPEVKLNYLKEEEDRRVAVDAMRFTRRIMAARALAKYEPEEYRPGI
                     AVEEDSDLERAAGELGTTIFHPVGTCKMGNDPMAVVDEELRVRGVERLRVIDASIFPR
                     ITSGNTNAPTYVIAEKGARAILKL"
BASE COUNT          464 a          766 c          748 g          392 t
ORIGIN      
        1 cgaaccagcg aaagatgcgc ggcaggacga gcaccgcgcc ctggcgctcc acgcgcacgt
       61 tgcgccgaat gtagtcggcg gcggcttcct ccagctgccg gtcgagcttg ccgccgtcga
      121 aagcgcgcag cggcggcgag gagcggcacg cagagtgcag cgcgaaatgc atgcgctcct
      181 cgaacacgat cggcatgtac tccaggcgcg cgtcgccgcg cttcatcggc ggctgcgagc
      241 ggcccggctt ggcgacattg ccgcgcagca gcccgtgctc gatgtcgtcg agtgaatagg
      301 aatgctcgcc tatccgcagt cgcggccgct cgaagaacgc ctccaccatc cgtacgccgg
      361 ctgccgactc gagctcgcgc gcgtcccgca gcacgccggc attgaacacg ttgatccaga
      421 acgcgagctg agatggaatt tgcgcccgct tgatgtcgaa gtccgcgagc gcgacaaggc
      481 aagcggcaag acgctcccgc gcgggcgagc gtgcgagcgc agcgtaatcg cacgccgcgc
      541 cgtgcaggtc gaaatgcgcc tgcctcgccg cctcgagcgc ttccgcgagc tcccggtcgg
      601 ggttgtccgc cacgctcatt acaatagcgc cggggaagcg aaccgttgca gaacttcggc
      661 gcattcgact acgtgatcgt cggcgcgggc agcgcgggct gtcttcttgc caacaggcta
      721 agccggaatt ccgagaaaaa agtcctcttg ctcgaggcgg gcgggcgcga cgactggttc
      781 tggatcgaca tcccggtcgg ctatctctac accatcgcca acccgcgcac cgactggtgc
      841 tacaagaccg agcccgacga gcatctcgcc gggcgctcga tccactatgc ccgcggccgc
      901 gtgctcggcg gctgctcctc gatcaacgcc atgatctata tgcgcgggca gaaggaagac
      961 tgggactact gggcctcgct cggcaaccgc gggtggtcat gggaggacgt tcttcccctg
     1021 tttctttccc tcgaggatta cgagcacggg gcggtcgacg gctacggcgc aggcggcgag
     1081 ctgcgcatcg aggacccgcg ggtgcgctgg gacatcatcg acgcctggcg ggaagccgcc
     1141 gccgaatgcg gcatcccgcc ggtcaaggtg ttcaaccgcg gcgacaactt cggctgcgcc
     1201 tacttccaga tgaaccagcg ccgcggccgg cgctggagcg cgaccaacgc ctttctgcgc
     1261 ccggtcacgc accggccgaa cttgacagta atgacgggcg cttcggtttc gaaattgata
     1321 tttgaaaacg ccacgtgcac aggcgttgag ttcgtccatc gtaacgagaa gttcagcgtg
     1381 caggcgacga aggaaacgat cctcgccgcc ggcgccatcg gctcgccgca gctcttgcag
     1441 ttaagcggaa tcggccctgc gggcctcctg gaaaaacatc agataaagct cttgcgtcac
     1501 ctgcccggtg tgggcgagaa cctgcacgac cacctgcaga tccgcatgca gtacaaggta
     1561 aagaacgtga agactctgaa cggcatcgcg aacagcctat ggggcaaggc ggcgatggca
     1621 ctggagtact tggtgttccg caccggcccg ctcaccatgc cgccgtcgca ggcgggcgct
     1681 tttgccagaa gcgatccggc gcagcccacg cccaacatcg agtggcacgt gcagcccctg
     1741 tcgctcgaca agttcggcga cccgctgcac agcttcccgg cgatcacgcc aagcgtgtgc
     1801 aacttgcgcc cagcctctcg cggatgggtg cgaataaagt cagccgatcc tgccgtgtat
     1861 cctgaggtaa agctcaatta cctgaaagag gaagaagatc gccgagttgc ggtggatgcg
     1921 atgcgcttta cgcgccgcat catggccgca agggcactgg cgaagtacga gccggaggaa
     1981 taccgccctg gaattgcagt cgaggaggat tcagacttgg aaagggcggc gggagagctc
     2041 ggcactacca tctttcaccc cgtcgggacc tgcaagatgg gaaacgaccc gatggccgtg
     2101 gtcgacgaag agctgcgcgt gcgcggggtg gagcggctac gcgtgatcga tgcctcaata
     2161 ttcccccgca tcacctccgg gaatacgaac gcgccgacct acgtgatcgc cgaaaagggt
     2221 gcgcgcgcaa tcttgaaact atagaatccg cccccaatct aagccccgga taaggagaac
     2281 aaatttccat gaagcgcatc ttcctgttcc tcgcgacgaa tatcgccgtc ctggtggtgc
     2341 tgtcggtcat catcaacatc ttcggcctcg
//
LOCUS       DASTPD010000005         9255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1429825, whole genome shotgun sequence.
ACCESSION   DASTPD010000005 DASTPD010000000
VERSION     DASTPD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429825"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFR57_00120"
     CDS             complement(<1..1135)
                     /locus_tag="VFR57_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0257814.1"
                     /translation="MIRSELKPRSEEFRGNAARMRALVWDLKQKVSVVTQGGDKAARD
                     KHTARGKMLPRERVAKLLDPGSPFLEIGQLAAWGMYGGDVHSASIICGIGRVCGRECV
                     IVANDATIKGGTYYPMTVKKHLRAQEIASQNRLPCIYLVDSGGGYLPEQDNVFPDREH
                     FGRIFFNIANLSAARIPQIACVMGSSTAGGAYVPAMCDESIIVKGQGTIFLGGPPLVK
                     AAIGEVVTPEELGGAEVHTRVSGVADHYALNDSHALSLVRRIVSGLNLRKQVSLEVRV
                     PREPLYPAEEMYGVIPADPRKPYDVRELIARIVDGSELDEFKHNYGTTLVTGFAHLHG
                     YPVGILANNGILFSESSLKATHFIELASQRGIPLLFLQNITGYM"
     gene            complement(1152..1730)
                     /locus_tag="VFR57_00125"
     CDS             complement(1152..1730)
                     /locus_tag="VFR57_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEU0257815.1"
                     /translation="MKPSIEFYFDFSSPYGYLASHKIEALAAKYARGVTWRPILLGVA
                     FKATGSGPLPSIPLKGDYAKRDFLRSARFHGVPFRMPEPFPVSTVAACRAFYSLNDPK
                     EQVLLAKALFRAYFIDNVNIGEASQVLKISNSLGLRPEIDSQAVKDKTRAEVEAALAK
                     GVFGSPYIVIDGEPFWGVDRFDQIERWLKEPF"
     gene            complement(1727..2854)
                     /locus_tag="VFR57_00130"
     CDS             complement(1727..2854)
                     /locus_tag="VFR57_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0257816.1"
                     /translation="MSAQAEEIRQAVRRFASEQLAPHAARWDREKIFPKEALKGLAEM
                     GLYGVAIAEEWGGAGLDYTSLAVACEEIAAGDCSTATIVAVNNLVAGILAGYASKKQK
                     EEFLKPLAQGKLLAAFALTEPQVGSDASAITTRAEKQGSHYVLNGVKQFITSGKNADI
                     AVVFAVTQKQAAKRGISAFLVPTSTPGYKVARIEDKAGQHASDTAQIVLENCKLPADL
                     LLSQEGVGYRIALANLESGRINVAAQATGVARAALEAAVAYARERKSMGKPLIGHQAV
                     NFRLADMATQVEAARQLYLHAARLRDEGKPCLKEASMAKLFASEMAEKVCSDAIQIHG
                     GYGYVTDFPVERLWRDVRVTKIYEGANDIQRLVIGRALEEG"
     gene            complement(2838..3167)
                     /locus_tag="VFR57_00135"
     CDS             complement(2838..3167)
                     /locus_tag="VFR57_00135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0257817.1"
                     /translation="MKINSAGCMLELPHKAALERPGGIINGPALMAAADCAMWLAVKA
                     RIGVENDALTSDLNTAFLAPAKGEHVYCTARILKMGRRRIYGVAECHGRKGNLFSHHT
                     LTYVRAS"
     gene            complement(3230..4414)
                     /locus_tag="VFR57_00140"
     CDS             complement(3230..4414)
                     /locus_tag="VFR57_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU0257818.1"
                     /translation="MQTDPIVIVGLARTPMGAFQGELKGFSAPELGAAAIRAAVERAK
                     IRPEEVQEVIMGCVLPAGQGQAPARQAALGAGLPLAVGCTTVNKMCGSGMKAAMFAHD
                     LLKSDSVDLVVAGGMESMTNAPYLLPKARAGLRMGHGQVLDHMFYDGLEDAYDKGRLM
                     GTFAEDCAAKYQFARAAQDEFAIQSLKRAQQANKQGWFAWEITPMAIKAGKEEKFVEN
                     DEQPFKANLEKIPTLKPAFRKDGTVTAANSSSISDGAAALVMMRRSAAQKRGLAPLAT
                     VVGHATHAQEPGWFTTAPVEAIRKLLERTHWNAKQVDLYEINEAFAVVTMAAMKEHGL
                     PHDKVNVHGGACALGHPIGASGARIAVTLIGALRKYGLKRGVASLCIGGGEATAMAIE
                     LA"
     gene            complement(4426..4872)
                     /locus_tag="VFR57_00145"
     CDS             complement(4426..4872)
                     /locus_tag="VFR57_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0257819.1"
                     /translation="MSAHSSGLIETYRGAVYPWHCDHMGHMNVMWYVGKFDEATWNLF
                     AAMGVTAAFLKENQRGMAAVQQNITYRRELLAGDTVLVRSAFLEAREKVAKFVHEMCN
                     AQTGEISAICVLTGVCVDAQTRKSCPFPRDLLARAQALVRPYDVPS"
     gene            complement(4869..6014)
                     /locus_tag="VFR57_00150"
     CDS             complement(4869..6014)
                     /locus_tag="VFR57_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEU0257820.1"
                     /translation="MNFLRFEHGETIDMLRESVRDFTAKEIAPRAAQIDETNEFPADL
                     WRKLGALGVLGITVEEEYGGTAMGYLAHIIAMEEISRGSASIGLSYGAHSNLCVNQIR
                     RNGTEAQKRQYLPKLVSGEHVGALAMSEPGAGSDVVGMRLRAERRGERYVLNGNKMWI
                     TNGPDADVLVIYAKTSDQGITAFLVEKAIKGFSTAQKLDKLGMRGSNTCELVFRDCEV
                     PSDNILGKEGAGVKVLMSGLDYERAVLAGGPLGIMAACLDVVIPYVHERKQFGQPIGE
                     FQLMQGKLADMYTTFSACRAYVYAVGQALDRNGTSRKDAAGAILYAAEKATWMAGQAI
                     QALGGMGYINETPTGRLWRDAKLYEIGAGTSEIRRWLIGRELFAETE"
     gene            complement(6011..6445)
                     /locus_tag="VFR57_00155"
     CDS             complement(6011..6445)
                     /locus_tag="VFR57_00155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0257821.1"
                     /translation="MNAPDPHFAARVRESFGRQKAMALIGASLTKVEPGEVEIALPYR
                     EDLTQQKGFVHGGILGMIADTACGYAAFSLMPAGGSLVTVEYKMNILKPGRGSLLAKA
                     KVIRPGRTLTVARGEVYADDGSHVATMLQTLMALPGRPDTPE"
     gene            complement(6492..6875)
                     /locus_tag="VFR57_00160"
     CDS             complement(6492..6875)
                     /locus_tag="VFR57_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257822.1"
                     /translation="METERAEFSISELAREFDVTPRAIRFYEDQGLLAPRREGQRRVY
                     SPRDRTRLKLTLRGKRLGLTLSEIRDLIDMYEPGRDERPQLERFLAVLESHKASLLQQ
                     QADLEAQLSEIQAFEKKVRRQLKRS"
     gene            6902..7942
                     /locus_tag="VFR57_00165"
     CDS             6902..7942
                     /locus_tag="VFR57_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0257823.1"
                     /translation="MSRQSISSLSYPFPEVPAPGTTIEVAPGIHWLSMPLPFKLDHIN
                     LWLIDEEEGGNADWTVVDTGIGNAETRALWEKILGKRHVKRVVLTHYHPDHAGNAAWL
                     CERYGADLWTTQGEYLTAHAVRTASAGYTPDAVLAVFRKNGLDEERAARMRGPRNRYA
                     DLVPDFPHAYRRIIEGDEITLGKHRWRAIVGHGHAPEHLSLYSKELNTVIAGDMLLST
                     ISTNVSVWSIDPEGDPLRLFLDSVARYRELPEDVLVLPSHGKPFRGAHRRVAELERHH
                     RERLEELTQSLKSPKSAAELLSVLFRRPLDAHQTFFAMGEAIAHLHYLYYAGRAKRAQ
                     GKDGIMRYATGG"
     gene            7980..>9255
                     /locus_tag="VFR57_00170"
     CDS             7980..>9255
                     /locus_tag="VFR57_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEU0257824.1"
                     /translation="MAERSSKILADFVQKQAETVSSAVRDEMGIAKAFMDLYARMATD
                     PAALATLSMNLWLDHMRLWQSSWMKLAGMDAAPVAEPDKGDSRFKDEDWSKNFLFDLI
                     KQSYLISSRHIREAVAKVDGLPGDSEKKVAFFTRQYVDALAPSNFLLTNPQVLRETVA
                     SGGQNLVRGLKNLLADLEKGGGQLRISMTDESAFQLGRNVATTPGKVVHQTDLMQLIQ
                     YAPLTGQAYRRPLVIIPPWINKYYILDLREKNSFIRWATEQGHTVFVLSWVNPDERLA
                     QKGFEDYMLEGPLAALDAVEKATGSKEINFIGYCLGGTLLGATLAYLKAKQQHKRVAS
                     ATFFVSLLDFSQPGELGVFIDEEQVVNLERKMNERGYLEGSEMAGTFNLLRANDLVWS
                     FVVNNYLLGKDPFPFDLLYWNADSTRMPARMHS"
BASE COUNT         1599 a         2936 c         2986 g         1734 t
ORIGIN      
        1 ccatgtagcc ggtgatgttc tgcaggaaca gcagcgggat gccgcgctgg ctggcgagct
       61 cgatgaagtg cgtcgccttg agcgaggact cggagaacag gatgccgttg ttggcgagga
      121 tcccgaccgg gtagccatgc aggtgcgcga agccggtgac gagcgtggtg ccgtagttgt
      181 gcttgaactc gtcgagctcc gagccgtcga cgatgcgggc gatgagctct ctcacatcgt
      241 agggcttgcg cgggtcggcg ggaatcacgc cgtacatctc ttccgcgggg tagagcggct
      301 cgcgcggcac ccggacctcg agtgagacct gctttctcag attgagtcct gagacgatcc
      361 ggcgcacaag cgaaagggcg tgcgagtcgt tcagtgcata gtggtcggcg acgcccgaga
      421 ctcgcgtgtg cacttcggcg ccgccgagct cctcgggcgt gacgacttcg ccgatcgccg
      481 ccttcacgag cggcgggccg ccgaggaaga tcgtgccctg gcccttgacg atgatcgatt
      541 cgtcgcacat cgcgggcacg tacgcgccgc ccgccgtgct cgagcccatc acgcacgcga
      601 tctgaggaat gcgcgccgcc gaaaggttgg cgatgttgaa aaagatgcgg ccgaagtgct
      661 cgcggtccgg gaacacgttg tcctgctcgg ggaggtagcc gccgcccgag tcgacgagat
      721 agatgcacgg cagccggttc tgggacgcga tctcctgcgc gcgcaggtgc ttcttcaccg
      781 tcatcggata gtaagtgccg cccttgatcg tcgcgtcgtt cgcgacgatc acgcactcgc
      841 ggccgcatac ccggccgatg ccgcagatga tggacgcgga gtgcacgtcc ccgccgtaca
      901 tgccccatgc cgcgagctgt ccgatctcga ggaagggcga gcccgggtcg aggagcttcg
      961 ccacgcgctc gcgcggcagc atctttccgc gcgccgtgtg cttgtcgcgc gccgccttgt
     1021 ctccgccctg ggtcacgacc gaaaccttct gcttcaggtc ccataccagt gcgcgcatgc
     1081 gggcggcgtt cccgcggaac tcctccgagc gcggcttgag ctcgctcctg atcaagacgg
     1141 ctccctgctt cttagaaggg ttccttcaac caacgctcga tctggtcgaa tcggtcaacg
     1201 ccccagaagg gctcgccgtc gatcacgatg tagggcgagc cgaagacacc tttggcgagc
     1261 gctgcttcga cttcggcgcg cgtcttgtcc ttgacggctt gactatcgat ctccggccgc
     1321 aagcccaggg agttggaaat cttcagaacc tggctggcct cgccgatgtt cacgttgtcg
     1381 atgaaatagg cccggaagag tgctttcgcc agcaatacct gctctttcgg atcgttcagg
     1441 gaatagaaag ccctgcaagc ggcaacggtg gagacgggaa acggctcggg catccggaac
     1501 gggacgccgt ggaagcgcgc cgagcgcagg aagtcgcgct tcgcgtagtc gcccttcagc
     1561 gggatgctgg gcagcgggcc gctccccgtg gccttgaagg cgaccccgag cagtatcggt
     1621 cgccaggtga cgccgcgagc gtactttgcc gcgagcgcct cgatcttgtg cgaggcgagg
     1681 tagccgtacg gggaggaaaa gtcgaaatag aactcgatgg acggcttcat ccttcctcca
     1741 gggctcttcc gatgacgagg cgctggatgt cgttggcgcc ttcgtagatc ttggttacgc
     1801 gcacgtcgcg ccatagccgc tcgacgggga agtcggtgac gtagccgtag ccgccatgga
     1861 tctggatcgc gtccgagcac actttttccg ccatctccga ggcgaaaagc ttggccatgg
     1921 acgcttcctt gaggcagggc ttgccttcat cgcgcagccg cgccgcgtgc aggtagagct
     1981 ggcgcgcggc ctcgacctgg gtcgccatgt cggccagcct gaaattgacc gcctggtgcc
     2041 cgatcagggg cttgcccatg ctcttgcgct cgcgcgcgta cgcgaccgcg gcctcgagcg
     2101 ccgcacgcgc aacgccggtt gcttgcgcgg cgacgttgat gcgccccgac tccaggttgg
     2161 cgagcgcaat acggtatcca acgccttctt gcgacaacag cagatcggca gggagtttgc
     2221 aattctccaa aacaatctgt gccgtgtcgg aggcgtgctg gccggccttg tcctcgatgc
     2281 gagccacttt gtagccgggc gtggatgtcg gcaccaggaa ggcgctgata ccgcgtttag
     2341 cggcttgttt ctgggttacg gcaaagacaa ccgcgatatc ggcgttcttc ccggaagtga
     2401 tgaattgctt gacaccgttc agcacgtagt gcgagccctg cttttcagcg cgcgtggtga
     2461 tcgccgaggc atcggagcca acctgcggct cggtgagcgc gaaggccgcg aggagcttcc
     2521 cttgggcgag gggcttgaga aactcctcct tctgcttctt gcttgcgtag ccagcaagaa
     2581 tcccggcgac gaggttattc accgcaacaa tggtcgccgt cgagcagtcg ccggccgcga
     2641 tctcctcgca cgcgacggcg agcgaggtgt agtcgagccc ggcgccgccc cattcctccg
     2701 ctatcgcgac gccgtagagg cccatctcgg cgaggccctt cagcgcctcc ttgggaaaga
     2761 tcttctcgcg gtcccagcgg gcggcgtgcg gcgcgagttg ctcgctcgca aatcgccgca
     2821 cggcctggcg gatctcttca gcttgcgcgg acataagtga gcgtatggtg gctgaagaga
     2881 ttgcccttcc ggccgtggca ctcggcgacg ccgtagattc tgcgccggcc cattttcaag
     2941 atgcgcgcgg tgcagtacac gtgctcgccc ttggccgggg cgaggaacgc ggtgttgagg
     3001 tccgaagtaa gcgcgtcgtt ctcgaccccg atgcgcgcct tgaccgcgag ccacatcgcg
     3061 caatcggccg ccgccatgag cgccgggccg ttgatgattc cgcccggtcg ctccagcgca
     3121 gctttgtgcg gaagctccag catgcagccc gcgctattta tcttcaccac acgaaaacca
     3181 taatggcgga ggaagcgcga tcggtcgagc agttgctgca acgctttttt caagcgagct
     3241 cgatcgccat ggccgtggct tctccaccgc ctatgcacag gctcgcgacg ccgcgcttga
     3301 gcccatactt gcgcagcgcg ccgatcagcg tcaccgcgat gcgtgcaccc gaggcgccga
     3361 tcgggtggcc gagcgcgcaa gcgccgccgt gcacgttcac cttgtcgtgg ggcagcccgt
     3421 gctccttcat cgcggccatg gtcacgaccg cgaaggcctc gttgatctcg taaagatcga
     3481 cctgttttgc gttccagtgg gttctttcga gcaatttcct tatggcttcc accggggcgg
     3541 tggtgaacca gccgggctcc tgcgcgtgcg tggcgtgccc gacgacggtg gcgagtgggg
     3601 cgagaccgcg tttttgcgcc gcggagcgcc gcatcatgac gagcgccgcg gcgccgtcgg
     3661 agatcgagct cgagttcgcc gcggttaccg tgccgtcctt gcggaatgcg ggcttgagcg
     3721 tcgggatctt ctccaggttg gccttgaacg gctgttcgtc gttctcgacg aacttctctt
     3781 ctttccccgc cttgattgcc atgggagtga tctcccacgc gaaccagcct tgcttgttcg
     3841 cctgttgtgc tcttttcaac gattgaatgg caaattcgtc ctgagctgca cgtgcgaact
     3901 ggtacttcgc ggcgcaatct tcggcgaacg tgcccatgag ccgccctttg tcgtaggcgt
     3961 cctccagacc gtcgtagaac atgtggtcga gcacttggcc gtggcccatg cgcaagccgg
     4021 cacgggcctt ggggagcagg tatggggcgt tagtcatgga ctccatgcca cccgccacaa
     4081 caagatcgac agaatcactt ttcaacaaat catgagcaaa catcgcggcc ttcatgccgg
     4141 agccgcacat cttgttcacc gtcgtgcagc cgacggcgag cggcaggccg gcgccgagcg
     4201 cggcctggcg cgcgggcgcc tggccctggc cagcgggcag cacgcagccc atgatgactt
     4261 cctgtacttc ctctggccgg atcttcgcgc gctcgacggc ggcgcggatc gcggcggcgc
     4321 cgagctcggg agcggagaag cccttgagct cgccctggaa ggcacccatt ggggtgcggg
     4381 caaggccaac gatgacgatt gggtcggttt gcatggaaat ctccttcatg aggggacgtc
     4441 gtacggtcgc acaagcgctt gagcgcgtgc gagcaggtcc cgggggaacg ggcacgactt
     4501 gcgggtctgg gcgtcgacgc agacgccggt cagcacgcag atcgccgaga tctcgccggt
     4561 ctgcgcgttg cacatttcat gaacgaactt cgctactttc tctcgtgcct cgaggaatgc
     4621 cgagcgcacg agcacggtat ccccggcgag gagctcccgc cggtaggtga tgttctgctg
     4681 gaccgccgcc atgccgcgct gattttcttt gaggaacgcc gcggtcacgc ccatggcggc
     4741 gaacaggttc caggtcgcct cgtcgaactt gccgacgtac cacatgacgt tcatgtggcc
     4801 catgtggtcg caatgccacg gatagaccgc gccgcggtag gtctcgatca acccggaaga
     4861 atgcgcgctc attcggtctc cgcgaagagc tcgcgcccga tcagccagcg ccggatctcg
     4921 gaagtgcccg cgccgatctc gtagagcttg gcgtcgcgcc agagtctgcc tgtcggcgtc
     4981 tcgttgatat agcccatgcc gccgagcgcc tggatcgcct ggcccgccat ccaggtcgcc
     5041 ttttcggcag cataaaggat cgcgccggct gcgtcctttc tgctcgttcc gtttctatcc
     5101 aaggcctgcc ccacggcgta gacataagcg cggcaggccg agaaggtggt gtacatgtcg
     5161 gcgagcttgc cctgcatgag ctggaactcg ccaataggct ggccgaactg cttgcgctcg
     5221 tgcacatacg ggattacgac gtcgaggcag gccgccatga tgccgagcgg accgccggcg
     5281 agaactgcgc gctcgtagtc gagaccggac atgagcactt tgacgccagc gccttctttt
     5341 ccaagaatgt tgtctgaggg cacttcgcaa tcgcgaaaca cgagctcgca ggtattggag
     5401 ccgcgcatgc cgagcttgtc gagcttctgc gcggtggaga agcctttgat tgctttctcg
     5461 acaaggaacg cggtgatccc ctgatcgctc gtcttggcat aaatcaccag cacgtcggca
     5521 tccggcccgt tcgtgatcca catcttgttg ccgttcagga cgtaacgctc gccgcgccgc
     5581 tcggcccgca acctcatgcc gacgacgtcc gagccggcgc cgggctcgga catggcgagg
     5641 gcaccgacat gctcgccgga aacgagcttc ggcaagtact ggcgtttctg agcctcggtg
     5701 ccgttgcggc ggatctggtt cacgcacagg ttcgagtgcg cgccgtacga caacccgatc
     5761 gaggcggagc cgcgcgagat ctcttccatc gcgatgatgt gggcgaggta gcccatcgcc
     5821 gtaccgccgt attcttcttc gaccgtgatg ccgagcactc caagggcgcc gagcttgcgc
     5881 cacaggtcgg cggggaactc gttggtttcg tcgatctgcg ccgcgcgcgg cgcgatctct
     5941 ttcgcggtga agtcgcggac ggattcgcgc agcatgtcga tcgtctcgcc atgctcaaat
     6001 cgcaaaaagt tcattctggt gtgtcggggc ggccgggaag cgccatcagc gtctgaagca
     6061 tggtagcgac gtggctgccg tcgtcagcgt acacctcgcc gcgcgccacg gtaagcgtgc
     6121 ggccggggcg gatgaccttc gctttcgcga gcagcgagcc gcggccgggc ttgaggatat
     6181 tcatcttgta ctcgacggtc acgagcgagc cgcccgccgg catgagggag aaggcggcgt
     6241 acccgcaggc cgtgtcggcg atcatgccca gaatgccgcc gtggacaaag cctttctgct
     6301 gcgtcaggtc ttcgcggtaa ggaagggcga tctccacctc gccgggctcc accttcgtga
     6361 gcgaggcgcc gatgagcgcc atggccttct ggcggccgaa gctttcgcgg acgcgggcag
     6421 cgaaatgcgg gtcgggggcg ttcacgggcc ttggttgggg gagaaaagcc tacttgacgt
     6481 ttacgttaac gtcaactacg cttgagctgc cgccgcacct tcttttcgaa cgcctggatc
     6541 tcggagagct gcgcctcgag atccgcctgc tgctgcagca ggctcgcctt gtgggactca
     6601 agcaccgcga ggaagcgctc gagctgtggc cgctcgtcgc gcccgggctc gtacatgtcg
     6661 atgaggtcgc ggatttcgga gagcgtgagg ccgagccgct tgccgcggag cgtgagcttg
     6721 agccgcgtgc ggtcgcgcgg cgaatagacg cggcgctggc cttcgcggcg gggcgcgagc
     6781 aggccctgat cctcgtaaaa gcggattgcg cgcggggtaa cgtcgaactc gcgcgcgagc
     6841 tcgctgatcg agaattcggc gcgctcggtt tccatggtaa cttcgatttt gattctagcg
     6901 gatgtcacgt cagtcgatct cttctctctc ttatcccttc cccgaagtcc cggcgcccgg
     6961 cacgacgatc gaggtcgcgc ccgggatcca ttggctctcg atgccgttgc cgttcaagct
     7021 cgaccacatc aacctgtggc tgatcgacga agaagaagga ggaaatgcgg actggaccgt
     7081 cgtcgatacc gggatcggca acgccgagac gcgcgccttg tgggagaaga tcctcgggaa
     7141 aagacacgtg aagcgcgtgg tgctcacgca ctaccatccc gaccacgccg gcaatgccgc
     7201 atggctctgc gagcgctacg gcgcggactt gtggaccacg cagggcgagt acctgaccgc
     7261 acacgcggtg cgcactgcga gcgccgggta cacgccggat gcggtgctcg cggtgttccg
     7321 caagaacggc ctggacgaag agcgcgccgc gcgcatgcgc gggccgcgca accgctatgc
     7381 ggacctggtg cccgacttcc cgcacgcgta ccgccgcatc atcgaaggcg acgagatcac
     7441 ccttggcaag caccgctggc gcgcgatcgt cggccacggc catgcgcccg agcacctgtc
     7501 tctgtactcg aaagagctca acacggtgat cgcaggcgac atgctccttt ccaccatctc
     7561 gaccaacgtg agcgtatggt cgatcgaccc cgagggcgat ccgctgcgcc tgttcctcga
     7621 ttccgtcgcg cgctaccggg agctccccga ggatgtgctc gtactgccct cgcacggcaa
     7681 gcccttccgc ggcgcgcatc ggcgggtggc ggagcttgaa cgacatcatc gggagcgact
     7741 ggaggaatta acccagtccc tcaagagtcc gaaaagcgcc gctgagctgc tcagcgtcct
     7801 tttccgcaga ccgcttgatg cgcatcaaac attctttgcg atgggcgagg cgatagccca
     7861 tttgcattac ctgtactatg ccggccgtgc aaaacgcgcc cagggtaaag atggcatcat
     7921 gagatatgca accggcggat aagacagaga cggaagaact agccaaggtc tgccacgacg
     7981 tggcggaacg ttcctccaag atcctcgccg acttcgtgca gaagcaggcc gaaaccgtgt
     8041 cctcggcggt gcgcgacgag atggggatcg ccaaggcgtt catggatctt tacgcccgca
     8101 tggcaaccga ccccgcagcg cttgcaacgc tatcgatgaa tctctggctc gaccacatgc
     8161 gcctgtggca gtcgagctgg atgaagctcg ccggcatgga tgcggcgccg gtcgccgagc
     8221 ccgacaaagg cgattcgcgc ttcaaggacg aggactggtc gaagaatttt cttttcgacc
     8281 tgatcaagca gtcctatctc atcagctctc gccatatccg tgaggcggtg gcgaaggtcg
     8341 acgggctccc tggggactcg gagaagaagg ttgcgttctt cacccggcag tatgtcgacg
     8401 cgctcgcgcc ctcgaatttc ctgcttacca acccgcaggt gctgcgcgag acggtcgcga
     8461 gcgggggcca gaacctggtg cgcggcctga agaacctgct cgccgacctc gagaaagggg
     8521 gcgggcagct gcgcatctcg atgaccgacg agagcgcttt ccagctcggc aggaatgtcg
     8581 ccaccacgcc cggcaaggtg gtgcaccaga ccgatctcat gcagctcatc cagtacgccc
     8641 cgctcaccgg gcaggcgtac cggcgcccgc tcgtcatcat cccgccctgg atcaacaagt
     8701 actacatcct cgacctgcgc gagaagaatt cctttatccg ctgggcgacc gagcagggcc
     8761 acaccgtgtt cgtcctctcc tgggtgaacc cggatgagcg cctggcgcag aaaggcttcg
     8821 aggactacat gctggaaggg ccgctcgccg cgctcgatgc ggtcgagaaa gcgaccggct
     8881 cgaaagagat caatttcatc ggctattgcc tgggcggaac gctcctcggc gcaacgctcg
     8941 cctaccttaa agcaaaacag cagcataagc gggtcgcaag cgcgactttc ttcgtaagcc
     9001 tgctcgactt ctcgcagccc ggcgagctcg gtgtgttcat cgacgaggaa caggtggtga
     9061 acctcgagcg caagatgaac gagcgtggct acctcgaagg ctcggagatg gccgggacgt
     9121 tcaacctgct gcgcgcgaac gatctggtgt ggtcgttcgt ggtgaacaac tacctgctcg
     9181 gcaaggaccc ctttccgttc gacctgctct actggaacgc cgattcgacg cgcatgccgg
     9241 cgcgcatgca cagct
//
LOCUS       DASTPD010000006         7808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1022080, whole genome shotgun sequence.
ACCESSION   DASTPD010000006 DASTPD010000000
VERSION     DASTPD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022080"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VFR57_00175"
     CDS             complement(<1..1479)
                     /locus_tag="VFR57_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257825.1"
                     /translation="MKSRWSDAEAARFTGQLGARIYTSRLLGADRSLVLHGGGNTSVK
                     LREKDLFGAEREVLYVKGSGWDLETIEAEGFVPLPLAYVQRLPLLPALSDAQMVNELN
                     THVLRAGAPSPSVETLLHALLPHRYVDHTHADAILSVSNAPDGEKRIREIYGERVAVI
                     PFAMAGFQLAALFAREYPRQASAKTIGVVLMSHGIVSFGAEGRESYERMIELVSVAEE
                     YLQKKKAWHILGAATTPSSVSREEIASLRRAISEQAGFPVVVKINDNAKFRAFAQRTD
                     VQELSQQGPATPDHLLFTKPVPMIGRDAAGYAGRYREYFERNVGTQGKSMLDPAPRMV
                     LDRELGFAAAGRDAREAAIIEELYDHTIDVILRAEALGGWRAVSERHLFDIEYWELEQ
                     AKLRRAGPAPLFRGEVALVTGAASGIGKACVQAFLRRGAAVAGLDRNPAIETAWLQPG
                     FLGVTVDLTDASAVDKALSRIVRAFGGVDMLVLNAGIFPSSQP"
     gene            1503..1919
                     /locus_tag="VFR57_00180"
     CDS             1503..1919
                     /locus_tag="VFR57_00180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0257826.1"
                     /translation="MPRIELLPWEEARAHASPIRFSVFVEEQGVPREIELDQHDPVCV
                     HAVVFEDGSAVATGRLLPDGHIGRMAVLKAWRGRGIGAAILNELMKAASKRGHREVAL
                     SAQVHALPFYRAHGFVPVGEKYLEAGIPHQEMKRAL"
     gene            complement(1916..2575)
                     /locus_tag="VFR57_00185"
     CDS             complement(1916..2575)
                     /locus_tag="VFR57_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEU0257827.1"
                     /translation="MCLIALAWRVHPEYPLIVAANRDEFHARPAAPAAFWDDYPEILA
                     GRDLEARGTWMGVSRSGRFGAVTNYRGARERRAVESRGALVSRFLLGVEKAGPYVDSL
                     KKDLYSGFNLLACDGEELWWTSNRNGAPRKLEPGVYGLANALLDSPDVEPAKRALDEA
                     VEPAPAVETLFQVLAKHRIVSPEYGTRCSTALLKGERTRYAERVFAPDGSEGETRHYE
                     F"
     gene            complement(2538..3452)
                     /gene="lsrF"
                     /locus_tag="VFR57_00190"
     CDS             complement(2538..3452)
                     /gene="lsrF"
                     /locus_tag="VFR57_00190"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEU0257828.1"
                     /translation="MADKDDIREGKDFGVGIPQQNRAFHVKGAGALDWGMQNRLARIF
                     NPASGRTVMLAFDHGYFQGPTTGLERIDLNIVPLIPYADVLMCTRGALRSVIPAAATN
                     ALVLRCSGGQSILTELSRELIAVDIEDAIRLNAAAITTQIYIGAEYEHQSIRNLVQLI
                     DTGNRYGIPTMAVTGVGAEMKRDERYFSLATRIAAELGAHYVKSYYIEEGFERVVAGC
                     PVPIVIAGGKKLPELDALTMAYSAVDQGAAGVDMGRNIFQSDDPAAMIQAVAVVVHKK
                     ERPEKAFQLYQQLKARNVPDRTRLARTS"
     gene            complement(3503..3769)
                     /locus_tag="VFR57_00195"
     CDS             complement(3503..3769)
                     /locus_tag="VFR57_00195"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEU0257829.1"
                     /translation="MNYYVYYKLAPEEVADMRGKVRALFAEVEEQFGIRGRWMRRRDD
                     PTTYMEVYEGIRDERAFDALLERFGANLGISRKIERFISAETPA"
     gene            complement(3766..4500)
                     /locus_tag="VFR57_00200"
     CDS             complement(3766..4500)
                     /locus_tag="VFR57_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEU0257830.1"
                     /translation="MECTRLSRYGLLAVIGADARDFLHAQLTNDIASLPSDRAALAGW
                     CTAKGRLLASMLVIPSPQGFLLQLARDLAPAIAKRLSMYVLRAKVKISDVSDAWAQYG
                     VWDAALKVAGVAWDGDVATVRVDEARFLRLAPADKFFPAPTKPENAWTLEEIRAGRPL
                     ISAATQDRFVPQMINFEALGAVSFQKGCYPGQEIVARAQYRGEVKRRMVHVQIPAGTE
                     LKPGQEFNGGVVVDSAAGEALAVMPV"
     gene            4530..5522
                     /gene="mltG"
                     /locus_tag="VFR57_00205"
     CDS             4530..5522
                     /gene="mltG"
                     /locus_tag="VFR57_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0257831.1"
                     /translation="MLKTLLGIVLLAAAAAAGYGAWYAGSPVRIAKYPVEVEIPRGAG
                     FRTAIEQLEKSGIAIRRYEFELLARAHGKVRDIKAGSYEILQPVTPLELLQKLTRGDV
                     TQAEVRLLEGWTFAQFRAALDASPHLRHETTGMSDADILARLNVTEMHPEGLFFPDTY
                     LFGKGASDLAVLRRSYGAMARHVKEEWEARDTTVPYRSPYEALIMASIIEKETGQAGE
                     RDMIGGVLVNRLRIGMRLQVDPTIIYGLGAAFDGNLKRSHLLEDGPYNTYTRAGLPPT
                     PIAMPGLASLRAAVRPGKTDAMYYVSRNDGSSHFSRTLEEHNRAVARYQLRGGR"
     gene            5525..6127
                     /gene="tmk"
                     /locus_tag="VFR57_00210"
     CDS             5525..6127
                     /gene="tmk"
                     /locus_tag="VFR57_00210"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0257832.1"
                     /translation="MRGKFVTLEGMDGAGKSTHQKFVADLVATRAPHVIQTREPGGTD
                     LAERLRQTILSDPMSPEVETLLLFAARADHVARVIRPALEAGTWVVCDRFSDATAAYQ
                     GAGKGVPADLIERLAAAAHPALVPDRTLVFDCSFEISRKRLQGKTLDRFEREERAFFE
                     RVRAAYLERSRAEPQRIRVVDAGLPLPEIRKALESALSDL"
     gene            6124..7014
                     /gene="holB"
                     /locus_tag="VFR57_00215"
     CDS             6124..7014
                     /gene="holB"
                     /locus_tag="VFR57_00215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU0257833.1"
                     /translation="MIHPWNEPILDALARRLERLPHALLIHGPRGVGKRVLAERISQL
                     LLCEGEKRPCDTCDACRWFRGGNHPDFRRVEPEFLWAEKPEKPSLVIKVEQVRELADF
                     LYIGSHRGRLRVALVHPAEEMNENAANALLKGLEEPPAGAIFILVSHRPAQLLPTIRS
                     RCVAVPAPLPPHNIALDWLAREGVTEPQRWLAYAGGAPLRATHYAAQGPVSLTPVGER
                     DGLEPLADALQKIALDRALMAFGLAAKYQTSPATVTREQARAWLAFARRMGEERLLCR
                     HPLNPRLFSAALLTRMPDLT"
     gene            complement(7101..7562)
                     /locus_tag="VFR57_00220"
     CDS             complement(7101..7562)
                     /locus_tag="VFR57_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEU0257834.1"
                     /translation="MIVFDLLCAEGHRFEGWFGSTEDFTAQKARSQLACPSCGSSRIE
                     RVPSASRFNLGAPEPKAPAAPAVAKRTPEMEGKDPFAIAQMLYSRMLDDLLSKSEDVG
                     REFPAEARRIYYQETSARAIRGQATQQEHQELVDEGIPVARLPVPPAGKLN"
     gene            7587..>7808
                     /locus_tag="VFR57_00225"
     CDS             7587..>7808
                     /locus_tag="VFR57_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU0257835.1"
                     /translation="MGKYRDDVAVLEHLVQPDRVHRDVYIDPELFQLEMERLWSRAWV
                     YACHASQLPNEGDYVTLDIAAQPLLVLRHG"
BASE COUNT         1332 a         2614 c         2533 g         1329 t
ORIGIN      
        1 cggctgcgag gagggaaaga tgcccgcgtt caggaccagc atgtccacgc ctccaaaggc
       61 gcggacgatc cggctcaaag ccttgtccac ggcgctggcg tcggtaagat ccaccgtaac
      121 gccgaggaag ccgggctgca gccacgcggt ttcaatcgcc gggttgcggt cgaggccggc
      181 caccgcggcg ccgcgccgca ggaacgcttg cacgcacgcc ttgccgatac ccgaggccgc
      241 gccggtcacg agcgccacct cgcctcggaa caacggcgcc ggccccgctc tgcgtagctt
      301 ggcctgctcg agctcccagt actcgatgtc gaagagatgc ctttcgctca ccgctcgcca
      361 gccgccgagc gcctcggcac gcaggatgac atcgatggta tggtcgtaga gctcctcgat
      421 gattgcggcc tcgcgcgcgt cgcggccggc agcggcaaaa ccaagctcgc gatccagcac
      481 catgcgcggc gcgggatcga gcatggattt tccctgtgtt ccgacgtttc tttcaaagta
      541 ttcgcgatag cgcccggcat accccgcggc atcccgccca atcattggga ccggcttggt
      601 gaagagcagg tgatcgggcg tcgccgggcc ttgctgcgac agctcctgca cgtccgtccg
      661 ctgcgcaaac gcccgaaact tcgcgttgtc attgattttc accacgacgg gaaacccggc
      721 ctgctcggag atcgctcgcc tcaggctcgc gatctcctct cggcttacag aagatggggt
      781 cgttgccgca ccgaggatat gccaagcctt cttcttctga aggtactctt ccgccacgga
      841 gaccagctcg atcatgcgct cgtaggactc gcgcccctcg gcgccgaagg agacgatgcc
      901 gtgcgacatc agcaccacgc cgatcgtttt ggcgctcgcc tggcgcgggt actcgcgcgc
      961 gaatagcgcg gcaagctgga atccggccat ggcgaagggg atgacggcaa cccgctcgcc
     1021 gtagatctcc cggatgcgct tctcgccgtc gggcgcgttg gacactgaga ggatcgcgtc
     1081 ggcgtgcgtg tggtcgacgt agcggtgcgg cagcagcgcg tgcagcagcg tttcgaccga
     1141 aggcgagggc gcgccggcgc gcagtacgtg cgtgttgagc tcgttcacca tctgggcatc
     1201 gctcagcgca ggcagcaagg gcaggcgctg cacgtaagcg agcggtagcg gcacgaagcc
     1261 ttcggcctcg atcgtctcca gatcccagcc gctgcccttc acataaagga cctcgcgctc
     1321 ggcgccgaaa aggtccttct cgcgcagctt caccgacgtg ttgccgccgc cgtggagaac
     1381 gagcgatcta tcggcgccga gcaggcgcga ggtatagatg cgagcgccga gctggccggt
     1441 gaaccgcgcc gcctcggcgt cgctccagcg gctcttcatg cggcgattat acttttcgca
     1501 ccatgccgag aatcgagttg ctcccctggg aggaagcgcg cgcacacgcg tcgccgatcc
     1561 gtttcagcgt gttcgtggaa gagcagggcg tgccgcgcga gatcgagctc gaccagcacg
     1621 atccggtctg cgtgcacgcc gtcgtgttcg aagatggcag cgcggtggcg accgggcgcc
     1681 tcctgcccga cggccatatc gggcgtatgg cggtgctcaa ggcctggcgt ggccgcggca
     1741 tcggcgctgc gattctcaat gaattgatga aagcggcgtc gaaaaggggg catcgggaag
     1801 tagccctgtc cgcgcaggtg catgcgctgc cgttctaccg cgcgcatggc ttcgttccgg
     1861 tgggcgagaa atacctggag gccggcatcc cgcaccagga gatgaaacgg gcgctttaga
     1921 actcgtagtg ccgcgtctcg ccctcgctgc cgtcgggcgc gaacacgcgc tcggcgtagc
     1981 gcgtgcgctc acccttgagc agggcagtgg aacagcgcgt gccgtactcg gggctcacaa
     2041 tccgatgctt ggcgagcact tggaacaacg tttccaccgc aggcgcgggc tcgaccgctt
     2101 cgtcaagagc tcgcttcgcg ggctcgacgt cgggggaatc aagcagggcg ttcgccaggc
     2161 cataaacgcc gggctcgagc ttgcgcgggg cgccgttccg gttggaggtc caccagagct
     2221 cctcgccgtc gcaggccagc aaattgaagc cgctatagag atctttcttg agggagtcta
     2281 cgtagggccc ggccttttcc accccgagca aaaagcgcga aaccaaagcg ccgcgtgatt
     2341 cgacggcacg ccgctcgcgc gcgccgcggt agttggtgac agcgccaaac cggccgctgc
     2401 gcgacacacc catccaggtg ccgcgcgctt cgagatcgcg gccggcgagg atttccgggt
     2461 aatcgtccca gaacgccgcg ggcgcggcgg gccgagcatg gaactcgtcg cggttcgccg
     2521 cgacgatcag cggatattca ggatgtacgc gccaggcgag tgcgatcagg cacattccga
     2581 gctttcagtt gctgataaag ctggaacgcc ttttcaggcc tctccttctt gtgcacgacg
     2641 accgccaccg cctggatcat ggccgccggg tcgtcggact ggaagatgtt gcgccccatg
     2701 tcgacgccgg cagccccctg gtcgacggcg ctgtaggcca tagtgagggc gtcgagctca
     2761 ggcagcttct tgccgcccgc gatcacgatc ggcaccgggc agcccgcgac cacgcgctcg
     2821 aagccctcct cgatgtagta gctcttgacg tagtgcgcgc cgagttcggc ggcgatgcgg
     2881 gtcgcgaggc tgaaatagcg ctcgtcgcgc ttcatttcgg cgccgacgcc ggtcaccgcc
     2941 atcgtcggaa tgccgtagcg gttccccgtg tcgatgagct gcaccaggtt cctgatcgat
     3001 tggtgctcgt attcggcgcc gatgtagatc tgcgtcgtga tcgccgcggc gttcaggcgg
     3061 atggcgtcct cgatgtcgac cgcgatcagc tcgcgcgaga gctcggtcag gatgctctgc
     3121 ccacccgagc agcgcagcac caaggcgttg gtggctgcgg ccgggatcac ggagcgcagc
     3181 gccccgcgcg tgcacatgag cacgtcggcg taggggatga gcgggacgat attcaggtcg
     3241 atgcgttcga ggcccgtggt cgggccctgg aagtagccat ggtcgaaggc cagcatcacc
     3301 gtgcggcccg aagccggatt aaaaatgcgc gcgagccggt tctgcattcc ccagtcgagc
     3361 gcgccggcgc ctttgacgtg gaatgcacgg ttctgctgcg ggatgccgac gccaaagtcc
     3421 ttgccttctc tgatatcgtc tttgtcagcc atagagccca accataccat gtacgtctac
     3481 gaaagcgtgg cggtgcgcgc tttcaagcag gtgtttccgc cgaaatgaag cgctcgatct
     3541 tgcggctgat tcccagattg gcgccgaagc gctcgagcag ggcgtcgaag gcgcgctcgt
     3601 cccggatccc ctcgtaaact tccatgtagg tcgtcgggtc gtcgcgccgg cgcatccaac
     3661 ggccccggat gccgaactgc tcctccactt ccgcaaagag cgcacgcacc ttgccgcgca
     3721 tgtcggcgac ttcttcggga gcgagcttgt agtagacgta gtagttcaaa ccggcattac
     3781 ggccagcgct tcgcccgccg cgctgtcgac caccacgccc ccattgaatt cctgccccgg
     3841 cttgagctcg gtgccggcag ggatttgaac gtgcaccatg cggcgcttga cctcgccgcg
     3901 atactgcgcg cgcgccacaa tttcctgacc ggggtagcag cccttctgga aactcaccgc
     3961 tccgagcgcc tcgaagttga tcatttgcgg cacgaaccta tcctgtgtcg ccgcgctgat
     4021 gagcggccgt ccggcgcgga tttcctcgag cgtccaggca ttttcgggct tggtcggcgc
     4081 aggaaaaaac ttgtcggcgg gtgctaagcg caagaagcgc gcctcatcca cccgcacggt
     4141 cgccacatcg ccatcccagg cgacgccggc gaccttaagc gcggcgtccc aaacgccgta
     4201 ctgggcccag gcgtcgctaa cgtcggagat tttcactttg gcgcgcaaca cgtacatgga
     4261 gagccgtttg gcgatcgcgg gtgcgagatc cctcgcgagc tgaagcagga agccctgagg
     4321 cgatggaatg acgagcatgc tcgcgagcag gcgtcccttg gcggtgcacc agccggcgag
     4381 cgcggcgcgg tcggaaggaa ggcttgcgat gtcgttcgtg agctgcgcgt gcaggaaatc
     4441 gcgcgcgtcg gcgccgatta ccgcaagcag tccgtagcga ctaagacgcg tgcactccat
     4501 cgtagaatga gcattttaca gtcgcgtcct tgctgaagac ccttctcggt atcgtgcttc
     4561 tcgccgccgc cgcggccgcc ggctacggag cgtggtacgc cggttcgccg gtcaggatcg
     4621 ccaagtaccc ggtggaagtc gagatcccgc gtggcgcggg cttccgcacc gccatcgagc
     4681 agctcgagaa gtcgggcatc gcgatccggc gctacgagtt cgagctgctg gcgcgtgctc
     4741 acggcaaggt gcgcgacatc aaggcgggca gctacgagat cttgcagccc gtgacgcccc
     4801 tcgagctgct gcaaaagctc acccgaggcg acgtcaccca ggccgaggtc cggctactcg
     4861 aaggctggac attcgcccaa ttccgcgccg cgctcgacgc gagcccgcac ctgcgccacg
     4921 aaaccacagg gatgagcgac gccgacatcc tcgcgcgact gaacgttacg gagatgcacc
     4981 ccgaggggct tttcttcccg gacacttatc ttttcggcaa aggcgcgagc gacctggcgg
     5041 tactgcggcg gagctacggc gcgatggcgc gccacgtaaa ggaggagtgg gaggcgaggg
     5101 acactacggt accgtaccgc tcgccctacg aagcgctcat catggcctcg atcatcgaga
     5161 aggagaccgg gcaggcgggc gagcgcgaca tgatcggcgg cgtgctggtg aaccgcctgc
     5221 gcatcggcat gcgactgcag gttgacccga ccatcatcta cggcctcggc gctgcgttcg
     5281 acggcaatct gaagagaagc cacctgctcg aggacggccc gtataacact tacacgcgcg
     5341 ccggcctgcc gccgaccccg atcgccatgc cggggctcgc ctcgctgcgc gccgccgtac
     5401 gcccggggaa gaccgacgcc atgtactacg tctcgcgcaa cgacggctcg agccatttct
     5461 cgcgcacgct ggaagagcac aaccgcgccg tggcgcgcta ccagctccgc ggcggacggt
     5521 aagagtgcgc ggcaagttcg tgactctgga aggcatggac ggggccggca agtcgacgca
     5581 ccagaagttc gtggccgact tagtcgccac acgcgccccg cacgtcattc agacgcgcga
     5641 gcccggcggc accgacctgg ccgagcggct tcgccagaca atcctctcgg atccaatgtc
     5701 gcccgaggtc gagacgctgc tcctcttcgc ggcgcgcgcc gatcacgtcg cgcgcgtgat
     5761 ccggccagcg ctcgaggccg gcacctgggt ggtctgcgac cgcttcagcg atgccaccgc
     5821 ggcgtaccag ggagcgggga agggcgtgcc cgccgacctg atcgagcggc ttgcggcagc
     5881 ggcgcacccg gccctcgttc ccgaccgaac gctggtgttc gattgctcgt tcgagatctc
     5941 caggaaaagg ttgcagggaa agaccctcga tcgattcgag cgcgaggaac gcgcgttctt
     6001 cgagcgtgtg cgcgccgcct acctcgagcg ctcgcgcgcc gagccgcagc gcatccgcgt
     6061 ggtcgacgcg ggcctgccgc tgcccgaaat tcgcaaggcg ctggaaagcg cgctttccga
     6121 cctgtgatcc atccctggaa cgagccgatt ctcgatgcgc ttgcgcgccg cctcgagcgg
     6181 ctaccgcacg cgctgctcat ccatgggccg cgcggcgtag gcaagcgtgt cctggccgaa
     6241 aggatttccc agctcctgct atgcgaaggc gagaagcggc cgtgcgacac gtgcgacgcc
     6301 tgccgctggt tccggggggg caaccatccc gattttcgcc gcgtcgagcc cgagtttctt
     6361 tgggccgaga agcccgagaa gccgagcctc gtcatcaagg tcgagcaggt gcgcgaactg
     6421 gcggactttc tgtatatcgg ctcacaccgc gggcgactgc gggtcgccct cgtgcatcca
     6481 gcggaggaaa tgaacgagaa cgccgccaat gcgcttctga agggcctgga ggagcccccg
     6541 gccggtgcaa tcttcattct ggtgtcgcat cgcccagccc agcttctacc gaccatccgc
     6601 agccgttgcg tcgctgtccc ggccccgctg ccgccgcaca acattgccct cgattggctc
     6661 gcccgggaag gcgttacgga gccgcagcgc tggctcgcct atgccggcgg tgcgccactc
     6721 cgggccacac attacgctgc ccagggtccg gttagcctca ctccggtggg ggagcgcgat
     6781 gggctcgagc cgctagcgga cgcgctgcag aaaattgccc tcgaccgagc cctcatggcg
     6841 ttcgggctgg ccgccaagta ccagacgagc cccgccaccg taacgcgcga acaagcccgc
     6901 gcatggctcg cattcgcgcg ccggatgggc gaggagaggc ttctttgccg gcatccgctg
     6961 aacccgcggc tcttttcggc ggcgctgctg acacgcatgc ctgatttaac ataatacaca
     7021 ttgtatgttt attgcctgta ccgcagcctc acgttcacat gctaatcagt tggcctgccg
     7081 gcctgccgag ccctcgatgc ctagttcaac ttaccggccg gcggcacagg caggcgtgcg
     7141 accggtatgc cttcgtccac cagctcctgg tgctcctgct gcgtagcctg gccgcggatc
     7201 gcgcgcgccg acgtttcctg gtagtagatg cgtcgagctt cagccgggaa ttcccgccca
     7261 acgtcctcgc tcttggaaag cagatcatcc agcatgcgcg aataaagcat ctgcgcgatc
     7321 gcgaacgggt ccttgccttc catctccggg gtgcgcttgg ccaccgccgg ggcagccggc
     7381 gccttgggct ctggcgcgcc caggttgaag cgcgaggccg aaggcacgcg ctcgatccgc
     7441 gaggagccgc agctcgggca cgcgagctgg gagcgcgcct tctgcgccgt aaagtcctcc
     7501 gtcgacccga accagccctc gaaccggtga ccctcggcgc aaagcagatc gaaaacaatc
     7561 atttgagtga ttctacaatg gcccggatgg ggaaataccg cgacgatgtc gcagtactcg
     7621 agcacctcgt gcagccggac cgggtccacc gcgacgtgta catcgacccg gagctcttcc
     7681 agctcgagat ggagcggctc tggtcacgcg cctgggtata cgcctgccat gcgagccagc
     7741 tcccgaacga aggcgactat gtcacgctgg acatcgccgc ccagccgctc ctcgtgctcc
     7801 gccacggc
//
LOCUS       DASTPD010000007         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_2673, whole genome shotgun sequence.
ACCESSION   DASTPD010000007 DASTPD010000000
VERSION     DASTPD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2673"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFR57_00230"
     CDS             complement(<1..702)
                     /locus_tag="VFR57_00230"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0257836.1"
                     /translation="MKICVIGAGAMGGSYGGLLARAGHDVTLVDTWAEHVAAIRANGL
                     IVEGVAGEHRVKVRGETAVPERLQADVVMIWTDSNHTREAAGAAAKALGNDGFAITLQ
                     NGIGNIESLVEVLGKGRVAGGSSMASAATRGPGRSVLTHMAATTIGELDGAESLRIGR
                     LRDALAGAGFDTRVHADIMAVIWTKFALNCSINALCATTGLRQGEMARLPAMGWLQDR
                     VIDEVLAVTRAKGLKL"
     gene            complement(712..2091)
                     /locus_tag="VFR57_00235"
     CDS             complement(712..2091)
                     /locus_tag="VFR57_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0257837.1"
                     /translation="MTQWLATNLALIMFVAMFFAIFCGFPVAFVMGGLALVFAVLGWM
                     LDIFPMLQISNIVLRMWGNVAIDPILVSIPMFIFMGSILERSGSAKDMLSAMEVMLKR
                     VPGALAVAVMAMGTILAAPMGVVGAAVVTLSVIALPQMLASGYDKQLAVGTISSAGTL
                     GILLPPAIMLVVMAEMLAVSAGTLFAAAVMPGLLLSGLYFLYIIILALVRPDKAPKLP
                     KEHGPQNWREFWVAMWKGLFPMTALLFIVLGSIFAGWATPTESAGVGVAGSMFIAWLN
                     RQMSWKMMNDAIRNACRANGLVFMIFLGATGFAYVFRVLGGDDLMISTLAKLGIETKW
                     EMLAFVMVLIFLLGFPFEWIEICLIVLPIFGPILGKYDFSDHLGEKYYFMAWFGTLVA
                     VNLQTSFMTPPFGATLFYMKGTAPPSITMSDIYQGMYPFVGLQILGLLLCIWYPEIAL
                     WLPRAVGFL"
     gene            complement(2101..2661)
                     /locus_tag="VFR57_00240"
     CDS             complement(2101..2661)
                     /locus_tag="VFR57_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0257838.1"
                     /translation="MRVLVRIADRIRAALEITAFAGGWLLILLALVTCFDVICRKIGI
                     EVPSTKLQEFEWHVHTALFALWMGYNYTVNAHPRVDSYTETLSLRGKAWIELLGCLIF
                     ALPYMAILCWYSIDFVAFAYNINEQSESGIGLTHRWIVKAVYAAGLWLVLIAILSVMM
                     RLIAYLFGRLPQDEVRLQIGPSKLEV"
     gene            complement(2711..3799)
                     /locus_tag="VFR57_00245"
     CDS             complement(2711..3799)
                     /locus_tag="VFR57_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0257839.1"
                     /translation="MNGTMHWSAAAVALSLAAASPMAYGQQKVNWKMQSVFGSQLTHL
                     GPSGQRFAKDVEEMTDGNFQIRFYEPNALVPGLECFDAASKGTVEACWTTPGFHAGRY
                     PALPFFTTVPFGPSYGEFFAWKIFGNGNKLRNEIYEKHGLVAFDSFAIGPETSGWFRR
                     EIKSLAELKGIKMRFFGLGARVMQKHGVQTQLLAPADIFPALERGVIDATEFSMPTMD
                     IKQGFHQVAKFNYFPGWHQQVSVSEVLMNKKAFDALPKSWKKIVEVAAGNQVHYTYAE
                     SEAMQFDAMAEMRDKHKVQIKRWSDEQIASFEKAWLEVIAEESAKDPLFKKIADDYLE
                     FRKKYAVWGDAQTMKPTYLPKGKPAAKP"
     gene            3846..4724
                     /locus_tag="VFR57_00250"
     CDS             3846..4724
                     /locus_tag="VFR57_00250"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257840.1"
                     /translation="MDTQRKPALGFIGLGQMGEPMVRRLLAAGFPVAVWNRSSNKVAP
                     ALEHGARAAASPAELARGADIVMTCVTDQRALEAVLFSPQGVAEGGGHGKLVVDFSSI
                     APDAARAFALRLERLGMGLVDAPVSGGVPGAEKGTLVVMAGGREEHVERVRPVLAHLA
                     SRVTRMGESGAGQATKLCNQIIVGALLPVLAEAVRLAEAAGVDATRLPEALKGGFADS
                     APLQIFGARMATRQFEPRLAAAALLKKDLENAAALARERGVTLPMVSAASELYRLLEA
                     QGRVQQDPSVLIELLR"
     gene            complement(4721..4906)
                     /locus_tag="VFR57_00255"
     CDS             complement(4721..4906)
                     /locus_tag="VFR57_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257841.1"
                     /translation="MVPHGSPELIREIHVVTFPNESAFAAYRKDERLAKFAHWREQSV
                     VQTELFVGEDGPVAAKP"
     gene            complement(5113..>5391)
                     /locus_tag="VFR57_00260"
     CDS             complement(5113..>5391)
                     /locus_tag="VFR57_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU0257842.1"
                     /translation="VFQALFGMIMTLLIAMEFKHSIVRVALRRSSIIQVKTVVLIALI
                     ALSRKFVILDSSATGASTIAALAGATLVLGVVYWLLRERDDRVAKEAQ"
BASE COUNT          967 a         1783 c         1740 g          901 t
ORIGIN      
        1 gagcttcaga cctttcgccc gggtcaccgc gagcacctcg tcgatgaccc ggtcctgcag
       61 ccagcccata gccggcaagc gggccatctc cccctgccgc aacccagtcg tcgcacagag
      121 cgcgttaatg ctgcagttga gcgcgaactt ggtccagatc acggccatga tgtccgcgtg
      181 gacgcgcgtg tcgaagccgg caccggcgag cgcgtcgcgc agcctgccga tgcgcagaga
      241 ctccgcgccg tcgagctcgc cgatcgtcgt cgctgccatg tgggttagca cggaacgccc
      301 cggcccgcgc gtcgccgcgc tcgccatgct cgagcccccc gcgacccggc ccttgccgag
      361 cacctcgacg agcgactcga tgttgccgat gccgttctgc agcgtgatgg cgaagccgtc
      421 attgccgagc gccttggcag ctgcgcccgc cgcttcccgg gtgtgattgc tgtcggtcca
      481 gatcatcacg acatcggcct gcaaacgctc ggggacggcg gtctcgcccc gcaccttcac
      541 gcggtgctcg cccgcgacgc cctccacgat caggccgttt gcacggattg ccgcgacgtg
      601 ctcggcccaa gtgtccacga gcgtcacgtc gtgcccggcg cgcgcgagca gcccgccgta
      661 cgagccgccc atggcgccgg ctccgattac gcagattttc attcgccgat cctagaggaa
      721 gccgaccgcg cgcggcagcc acagcgcgat ttcggggtac cagatgcaga gcagcaggcc
      781 caggatctgc agcccgacga aggggtacat gccctggtag atgtcgctca tggtgatgct
      841 gggcggcgca gtgcctttca tgtagaagag cgtcgcgccg aatggcgggg tcatgaacga
      901 ggtctgcaga ttcaccgcca ccagcgtgcc gaaccaggcc atgaagtagt acttctcgcc
      961 gaggtggtcg gagaagtcgt acttgccgag gatcgggccg aagatcggca gcacgatcag
     1021 gcagatctcg atccactcga acgggaagcc gagcaggaag atgagcacca tgacgaaggc
     1081 gagcatctcc cacttggtct cgatgccgag cttggcgagc gtggagatca tcaggtcgtc
     1141 gccgcccagc acgcggaaca cgtaggcgaa gccggtggcg ccgaggaaga tcatgaacac
     1201 cagcccgttc gcgcggcagg cgttgcggat cgcgtcgttc atcatcttcc agctcatctg
     1261 ccggttgagc caggcaatga acatggatcc cgcgacgccg accccggcgc tctccgtcgg
     1321 cgtcgcccat ccggcgaaga tggagccgag cacgatgaag agcagcgcgg tcatcgggaa
     1381 caggcccttc cacatcgcga cccagaactc gcgccagttc tgcggcccgt gttccttcgg
     1441 caacttcggc gccttgtccg gccgcaccag cgcgagaatg atgatgtaga ggaaatacag
     1501 cccggacagc agcagccccg gcatgactgc ggcggcgaac agcgtccccg ccgataccgc
     1561 gagcatttcg gccatcacta ccagcatgat cgccggcggt agcaggatcc cgagagtgcc
     1621 ggcggacgag atcgtgccga ctgcgagctg cttgtcgtac cccgaggcga gcatttgcgg
     1681 cagcgcgatc accgaaagcg tgaccacggc cgcgccgacc acgcccatcg gcgcggcgag
     1741 gatggtgccc atcgccatca ccgcgacggc aagcgcgcct ggcacgcgct tgagcatcac
     1801 ctccatcgcg ctgagcatgt ccttggccga gcccgaacgc tcgaggatcg accccatgaa
     1861 gatgaacatc gggatcgaga ccaggatcgg gtctatggcg acgttgcccc acatgcgcag
     1921 cacgatgttc gagatttgca gcatggggaa gatgtcgagc atccagccga gcaccgcgaa
     1981 caccagcgcc aacccgccca tgacgaatgc caccgggaag ccgcagaaga tggcgaagaa
     2041 catcgccacg aacatgatga gcgcgaggtt ggtggcgagc cattgcgtca tggcggcggc
     2101 ctacacttcc aacttcgacg ggccgatctg caggcgaacc tcgtcctgcg gcaggcgccc
     2161 gaacaggtat gcgatcaagc gcatcatgac gctcaggatg gcgatgagga ccagccacag
     2221 cccggcggcg tagaccgcct tgacgatcca gcggtgcgtg aggccgatcc cgctctcgga
     2281 ctgctcgttg atgttgtagg cgaaggccac gaagtcgatg ctgtaccagc agaggattgc
     2341 catgtacggc aatgcgaaga tcaggcagcc gagcaactcg atccacgcct tgccgcgcag
     2401 gctgagcgtt tcggtgtacg agtcgacgcg cggatgcgcg ttgacggtgt agttgtagcc
     2461 catccatagc gcgaacagcg ccgtgtgcac gtgccactcg aactcctgca gcttggtcga
     2521 cggcacctcg atgccgatct tgcggcagat gacatcgaaa cacgtcacga gtgccagcag
     2581 gatgagcagc cagccgccgg caaatgccgt gatctccagc gcggcgcgga tgcggtcggc
     2641 gatgcggacg aggactctca tgcgcacaaa aaaggccggg cgggcccggc ctccgtggct
     2701 ctgctgccgg ctagggcttc gccgccggct tgcccttggg cagatacgtc ggcttcatcg
     2761 tctgcgcgtc gccccacacc gcgtacttct tgcggaactc gaggtagtcg tcggcaattt
     2821 tcttgaacag cggatccttg gcggattcct cggcgatcac ctcaagccag gccttctcga
     2881 acgaggcgat ctgctcgtcg ctccagcgct tgatctgcac cttgtgcttg tcgcgcatct
     2941 cggccatggc gtcgaactgc atcgcctcgg actcggcgta ggtgtaatgc acctggttgc
     3001 ccgccgcgac ctcgacgatc ttcttccagg acttcggcag cgcgtcgaac gccttcttgt
     3061 tcatcagcac ttcgctcacc gacacctgct ggtgccagcc ggggaagtag ttgaacttcg
     3121 ccacctggtg gaagccctgc ttgatatcca tggtcggcat cgagaactcg gtggcgtcga
     3181 tcacgccgcg ctcgagcgcc gggaagatgt ccgcgggcgc gagaagctgc gtctgcacgc
     3241 cgtgcttctg catcacgcgc gcgcccaggc cgaagaagcg catcttgatg cccttcagtt
     3301 cggcgagcga cttgatctcc cggcggaacc agcccgaggt ctcgggcccg atggcgaagg
     3361 agtcgaaggc gaccaagccg tgcttctcgt agatctcgtt gcgcagcttg ttgccgttgc
     3421 cgaagatctt ccaggcgaag aactcgccgt agctcggacc gaagggcacc gtcgtgaaga
     3481 acggcagcgc cgggtagcgg ccggcgtgga aacccggcgt ggtccagcac gcctcgaccg
     3541 tgcccttgga ggcggcgtcg aagcattcga ggccgggaac gagggcattg ggctcataga
     3601 agcggatctg gaaattgccg tcggtcatct cctccacgtc cttggcgaag cgctgcccgg
     3661 acgggccgag gtgtgtcagc tggctgccga acacgctttg catcttccag ttgaccttct
     3721 gctggccgta cgccatggga gacgcagcgg ccagcgagag cgccactgcc gcggcgctcc
     3781 agtgcatggt tccattcatg ttgccctcct cctaaggttg gcgcagccat cataatgacc
     3841 gtacggtgga tacacaacga aagcccgcac tgggcttcat tggcctgggc cagatgggcg
     3901 agccgatggt gcgccgcctc ctcgcggcgg gctttccggt cgcggtgtgg aaccgctcaa
     3961 gcaacaaggt cgcccccgcg ctcgaacatg gcgctcgggc ggcagcctcg ccggccgagc
     4021 tggcgcgcgg cgccgacatc gtcatgactt gcgtgacgga ccagcgggcg ctggaagcgg
     4081 tcctgttcag tccgcaaggc gtcgccgagg gcggcggtca cggcaagctg gtggtcgact
     4141 tctccagcat cgcgccggat gcggcgcgcg cttttgcgct gcgcctggaa cgccttggaa
     4201 tggggctcgt cgacgcgccg gtgtcgggcg gcgtgcccgg ggccgagaaa ggcacgctgg
     4261 tggtcatggc gggcgggcgc gaggagcacg tcgagcgcgt gcgcccggtg ctagcgcacc
     4321 tggcgagccg cgtcacccgc atgggcgaga gcggcgccgg ccaggcgacc aagctgtgca
     4381 atcagatcat cgtcggcgcc ttgctgccgg tcctggccga ggccgtgcgc ctcgccgaag
     4441 ccgccggcgt cgatgcgacc cggttgccgg aagcgctaaa aggcgggttc gccgattccg
     4501 cgccgctgca gatcttcggc gcgcgcatgg cgacgcgcca gttcgagccg cgcctcgccg
     4561 ccgcggcgct cctcaagaag gacctcgaga acgccgcggc cctggcgcgc gagcgcggcg
     4621 tcactcttcc catggtgagc gccgcctccg agctctatcg cctgctcgag gcgcagggcc
     4681 gcgtccagca ggacccttcg gtcctgatcg agctcctgcg ttagggtttc gccgccacgg
     4741 ggccgtcctc tccgacgaaa agctcggttt ggaccacgga ctgctcgcgc caatgggcga
     4801 acttcgcgag ccgctcatcc tttcgatacg cggcgaacgc gctctcgttc ggaaacgtta
     4861 ctacgtgaat ctccctgatc agctccggtg acccgtgcgg aaccacgaca actctctcta
     4921 actgtccccc atgcctcttc attacttcag ccgcgtgcgc ttcgaaggcg cgaaacctat
     4981 caatggcgtc cttccgcact gtaagaatcg caacgagggt catcgggcgt aacggtcccg
     5041 gctaccagct aacgtcgtgg tgaacggcgg gccgcagcga cggtctttgc gttggcacgt
     5101 agcgcgaaaa gctcattgcg cctccttggc gacacggtca tcacgctcgc gcagcagcca
     5161 gtagaccacg ccgagcacaa gggttgctcc agccagcgcc gcaatggtgc ttgcgccggt
     5221 ggcgctggaa tcgagaatga cgaacttgcg actcaacgca atgagcgcga tgagcaccac
     5281 ggtcttgacc tggatgatgc tcgagcgccg aagagcaacg cgaacaatcg aatgcttgaa
     5341 ttccatggcg atgagcagcg tcatgatcat gccaaagagc gcctgaaaga c
//
LOCUS       DASTPD010000008         6831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1024351, whole genome shotgun sequence.
ACCESSION   DASTPD010000008 DASTPD010000000
VERSION     DASTPD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024351"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFR57_00265"
     CDS             complement(<1..698)
                     /locus_tag="VFR57_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0257843.1"
                     /translation="MATSTIESVLQEKRVFPPAPAFVKQANISGMDAYRKMVAEFERD
                     FEGTWGRLARETLDWAKPFTKVLDESKAPFFRWFHDGELNASYNCLDRHLKTQPEKVA
                     IIFEADDGKVSKITYRELHKEVCRFANALKSHGVKMGDRVLIYMPMSIQVVVAMQACA
                     RIGATHSVVFGGFSAKSLQERIIDAGATEVITADGQFRGGREIPLKPVVDEAFGMGGC
                     EKVKNVIVYRRSGS"
     gene            916..2436
                     /locus_tag="VFR57_00270"
     CDS             916..2436
                     /locus_tag="VFR57_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0257844.1"
                     /translation="MTSIRREHLVQSIADALQFISYYHPADYIRALGRAYEREQSPAA
                     RDAIAQILTNSRMCAEGRRPICQDTGIVNVFLKIGMQVRFDFEDLENAVNEGVRQAYL
                     DPDNKLRASVLDESDFGRKNTRDNTPAVISVQMVPGEQLEVDVAAKGGGSEAKAKFAM
                     LNPSDSIADWVLKTVPTMGAGWCPPGMLGIGVGGTAEKAMLMAKEALMEPIDMAELKA
                     RGPKNKVEELRIELYDKVNALGIGAQGLGGLSTVLDVKIRTYPAHAANRPVAIIPNCA
                     ATRHAHFVLDGSGAAEFQAPSLSDWPAVTWTPDAKSKKVDLNSLAKAEVATWKPGERL
                     LLSGKLLTGRDAAHQRIQDLLEKNKPLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTA
                     TRMDKFTDLMLAKTGLAAMIGKGERGPAAVEAIRRHKAASLIAVGGAAYLVAKAIRKS
                     RVVAFEDLGMEAIYEFEVQDMPVTVAVDSAGQSVHQSGPAEWSQKIFELKIPVTAA"
     gene            2433..3605
                     /locus_tag="VFR57_00275"
     CDS             2433..3605
                     /locus_tag="VFR57_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257845.1"
                     /translation="MSNWPAIWAIFAGGLVAGAYMTKVAPALPGLRAELGLTLVESGF
                     IATTFNVMGMLVGMLAGVLCDRFGHKRLALAGLCVLFVGGILGAGAWGFGSLLFGRFV
                     EGIGFILFTVSAVALMSASAATPRDRAKALGLWSAYMPTGGAIALFAAPWPIASWGWR
                     GLWMVLAVASVLSAIAVARLVPVSRYGQVSSMRLVVESLMRSGNIAMAVLFAFYVAQW
                     TSIMIWLPTFLIDEHRMAAATAALATAAYVLINAPGNLAGGWLLAHGVPRGALIVGGA
                     ALAALCEVGMLSDAFPAGLRYTLVLVFSFCAGVIPASVFSGLPIHARTPQHIGTGNGI
                     VMQASQIGQFLGPLAIAWMATRFGGWHASLWVLLAFAGGAALCGVAIGAIENRIKS"
     gene            3602..4525
                     /locus_tag="VFR57_00280"
     CDS             3602..4525
                     /locus_tag="VFR57_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU0257846.1"
                     /translation="MNRQVMAREVHICEVGPRDGLQNAKHLMPTEAKKVWISALAAAG
                     LEEIEVGSFVPPRLIPAMADTGEIVVFARTIPNLKVVALAPNLKGFQRALEAGAHKVT
                     FPVSASRAHSESNVRMTPDQMVEEVRKCAQRPHPGVEIEGAVSTAFGCTLQGTVPEEE
                     VVRIAKALAEFCDTVALADTVGYANPAQVKRLFNKVKAQIGDKLAGAHFHNTRGLGLA
                     NALAAYEEGVRHFDSSMGGLGGCPFAPGASGNVITEDLVFMFESMGVPTGISLEKLFQ
                     AREILVKGIPEEPIYGHTPLAGLPKGFRAAA"
     gene            4522..5319
                     /locus_tag="VFR57_00285"
     CDS             4522..5319
                     /locus_tag="VFR57_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0257847.1"
                     /translation="MSEAAVQTATAGGVCTITLNRPAALNALNQDLAIGLRDAVLAAE
                     QDQSARCLVIRGGQNFMAGGDLKWFDDLVRSRRPEQNRIEFERFAGDVHSVIVSLRRM
                     PKPVLASVRGAAAGFGMSLMMACDLVIAADNAYFSLAYSLIGASPDGGSTFALPRIVG
                     AKKAMEIALLSERFDAATAERLGLVNRVVPAAALDDETARLAARLAGGPTAVYGRTKQ
                     LLNASLNSSLESQLQREAEAFAQSASEPAFAEGVRAFIEKRKPLWPR"
     gene            5307..6125
                     /locus_tag="VFR57_00290"
     CDS             5307..6125
                     /locus_tag="VFR57_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257848.1"
                     /translation="MASLKGKTLFITGGSRGIGLAIALRAAADGANLVIAAKTVEPHP
                     KLPGTIHTAAREIEAAGGRCLPLQCDIRDEAQLARAVAAAAERFGGIDILVNNASAIS
                     LSGTMETPLKRLDLMFGINVRGTYASSQACVPHLRKAANPHILTLSPPLNMNPRWFKN
                     HVAYTMAKYGMSMCVLGMAEEFRAAGIAVNALWPKTIIATAALQMIPGIDPTRGRKPE
                     IMADAAHWILTQPARACSGNFFIDEEVLRKAGVTDLRQYAVDPSKDLLPDLFLD"
     gene            6125..>6831
                     /locus_tag="VFR57_00295"
     CDS             6125..>6831
                     /locus_tag="VFR57_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0257849.1"
                     /translation="MLPLEGIRVVEFAHMVMGPSCGLVLADLGAEVIKVEPLAGDNTR
                     RLEAAGAGFFPVFNRNKKSLAIDLQKNEGKQIVKRLLATADVLTENFRPGALDKLGFS
                     FDSLKTINPRLIYCSLKGFLKGPYEHRLALDEVTQMMGGLAYMTGLPERPLRAGSSVV
                     DIMGGTFAAVGILAALREREKTRRGTRVTSSLFESTAYLVAQHMAQFELTGEAPPPMS
                     VKRPAWGIYDIFDTAGGG"
BASE COUNT         1165 a         2245 c         2232 g         1189 t
ORIGIN      
        1 tggctgccgc ttcgccggta cacaatgacg ttcttcactt tctcgcaccc gcccatgccg
       61 aaggcctcgt cgaccacggg cttgagcggg atctcgcgcc cgccgcgaaa ctggccatcg
      121 gcggtgatca cctcggtggc gccggcatcg atgatgcgct cctgcaggct cttggcggag
      181 aagccgccga ataccaccga gtgggtcgcg ccgatgcgcg cgcacgcttg cattgccaca
      241 acgacctgaa tcgacatcgg catgtagatc aggacgcgat cgcccatctt cacgccgtgg
      301 gatttgagag cattcgcgaa gcggcagacc tccttgtgca gctcgcgata ggtgatcttg
      361 ctgactttgc cgtcgtcggc ctcgaagatg attgcgacct tctcaggctg ggtcttgaga
      421 tgccggtcga ggcagttgta ggaggcgttg agttctccgt cgtggaacca gcggaagaac
      481 ggcgccttgg actcgtcgag caccttggtg aagggcttgg cccagtcgag cgtctcgcgc
      541 gccaaccggc cccacgtgcc ttcgaagtcg cgctcgaact ccgcgaccat cttgcggtag
      601 gcgtccatgc cggagatgtt tgcctgcttg acgaaggcgg gcgcaggcgg gaacacccgt
      661 ttttcctgca gcaccgattc gatcgtcgat gtggccatgc gctagtcctc ctccaaggtc
      721 tgcgatccat cttgcgacgg tggtcttaca ccgcgcttac ggcaattaca atgatacagt
      781 tccacgcccg cgttcaagcg aaagacggat ggctcagaaa tcccccgttg tgaccgaggg
      841 tcgcgccttg cggccgctcc cctcggccat ggcggtcgcc gcggccgacc gcatctttcc
      901 gcaggcaccg cacgcatgac ctccattcga cgcgagcatc tagtgcaaag catcgccgac
      961 gctctgcagt tcatctccta ctatcacccg gccgactaca tccgcgccct cggccgcgct
     1021 tacgagcgcg agcaatcccc cgcggcacgc gacgcgatcg cccagatcct gaccaactcg
     1081 cgcatgtgcg ccgaaggccg ccgccccatc tgccaggaca cgggcatcgt caacgtgttc
     1141 ctgaagatcg gaatgcaggt caggttcgac ttcgaggatc tggaaaatgc cgtgaacgag
     1201 ggcgtccgcc aggcttacct cgaccccgat aacaagctgc gcgcttcggt gctcgacgag
     1261 tcggacttcg ggcgcaagaa caccagagac aacacacctg ccgtgatcag cgtgcaaatg
     1321 gtgcccggcg agcagctcga ggtcgacgta gccgccaagg gcggaggctc ggaggcgaag
     1381 gccaagttcg cgatgctcaa tccctcggac tcgatcgccg actgggtgct gaagaccgtc
     1441 ccgaccatgg gcgcgggctg gtgcccgccg ggcatgctcg gtatcggcgt cggcggcacg
     1501 gccgagaagg cgatgctgat ggcgaaggag gctctcatgg agccgatcga catggccgag
     1561 ctcaaggcac gcggaccaaa gaacaaggtc gaggagctgc gcatcgagct ctacgacaag
     1621 gtgaacgcgc tcggcatcgg cgcacaaggc ctgggcggcc tttccaccgt cctcgacgtc
     1681 aagatccgca cctaccccgc acacgccgcg aaccgtccgg tcgccatcat tcccaactgt
     1741 gccgcgacgc ggcacgcgca cttcgtgctc gacgggtccg gcgccgcgga attccaagcg
     1801 ccctcgctct cggactggcc ggcggtgacg tggacgccgg atgcgaagtc gaagaaggtc
     1861 gatttgaatt cacttgccaa agcggaggtc gccacttgga agccgggcga gcgcctcctc
     1921 ctctcgggca agctcctcac cgggcgcgac gccgcgcacc agcggattca ggacttgctg
     1981 gaaaagaaca agccgctccc cgtcgatttc accaaccgcg tcatctacta cgtcgggccg
     2041 gtcgatccgg tgcgcgacga ggtggtcggt cccgcgggtc cgacgacggc tacgcgcatg
     2101 gacaagttca ccgacctcat gcttgcgaag accggccttg cagcgatgat cggcaaaggc
     2161 gagcgcgggc cggccgcggt cgaggcgatc cgcaggcaca aggcggcttc cctcatcgcc
     2221 gtcggcggcg cggcgtacct ggtggcgaag gcgatccgca agtcgcgcgt ggtagcgttt
     2281 gaggacctcg ggatggaagc gatctacgaa ttcgaggtgc aggacatgcc ggtcactgtc
     2341 gctgtcgatt cggccggtca atccgtccac cagtccgggc cggccgagtg gtcgcaaaag
     2401 atctttgaac tgaagattcc tgtcactgcc gcttgagtaa ctggcccgcg atctgggcca
     2461 tcttcgccgg cgggctggtt gccggcgcct acatgacgaa ggtcgccccg gcgctgcccg
     2521 gcctgcgcgc agagctcggc ttgacgctcg tcgagtccgg cttcatcgcc actacgttca
     2581 acgtgatggg catgctggtc ggcatgctcg ccggcgtgct gtgcgaccgc ttcgggcaca
     2641 agcggcttgc gcttgcgggt ctctgcgtgc tcttcgtcgg aggcatcctg ggcgcaggcg
     2701 cctggggctt cggaagcctc cttttcgggc gcttcgtcga aggcatcggc ttcatccttt
     2761 tcacggtctc tgccgtggcg ctgatgagtg cttcggccgc cacgccgcgc gatcgcgcca
     2821 aggcgctcgg actgtggagc gcctacatgc cgacgggagg tgcgatcgcc ctcttcgccg
     2881 cgccgtggcc gattgcttcc tggggttgga gaggactctg gatggtcctc gcggtcgcat
     2941 ccgtcctatc ggcgattgcc gtggcccgcc tggtcccggt gtcgcgctac ggccaagtga
     3001 gctcgatgcg cctcgtcgtc gagtccctga tgcgcagcgg caacatcgcg atggcagtcc
     3061 ttttcgcttt ctacgtggcg cagtggacgt cgatcatgat ctggctgccg actttcctca
     3121 tcgacgagca tcgcatggcg gccgctacag ccgcgctcgc gacggcggcc tacgttttga
     3181 tcaatgcccc gggcaacctc gccggcggct ggctgctcgc gcacggtgtg ccgcgcggtg
     3241 cgcttatcgt cggcggggct gcgctcgccg cgctgtgcga ggtcgggatg ctgtcggatg
     3301 ccttccccgc cggcctgcgc tacacgctgg tgctcgtctt ttccttctgc gccggggtga
     3361 tcccggcctc ggtgttttcc gggctgccga tccacgcgcg cacgccgcag catatcggta
     3421 ccggtaacgg catcgtcatg caggcctcgc agatcggaca gttcctcggc ccgctggcga
     3481 tcgcctggat ggcgacgcgc ttcggcggat ggcacgcgag cctgtgggtc cttcttgctt
     3541 tcgccggcgg cgctgcgctc tgcggcgtcg caatcggagc gatcgagaat agaatcaagt
     3601 catgaaccga caagtcatgg cgagagaagt gcacatttgc gaagtagggc cccgtgacgg
     3661 cctccagaac gcaaagcacc tgatgcccac ggaggcgaag aaagtctgga tttccgctct
     3721 ggccgcggcc ggactggagg agatcgaggt cggctcgttc gtcccgccca ggctgatccc
     3781 ggcgatggcc gataccggcg agatagttgt tttcgcaaga accatcccga acctaaaagt
     3841 cgtggcgctt gcgccgaacc tgaagggctt ccagcgcgcg ctcgaagccg gagcgcacaa
     3901 ggtcaccttt ccggtgtcgg cgagccgggc gcacagcgag tcgaacgtgc gcatgacacc
     3961 cgaccagatg gtcgaggaag tgcgcaagtg cgcgcagcga ccacatcccg gcgtggagat
     4021 cgaaggcgcc gtgtcgaccg cgttcggctg cacattgcag ggcacggtgc ccgaggaaga
     4081 ggtcgtgcgc atcgcaaagg cgctggccga gttctgcgac acggtggcgc tcgcggatac
     4141 cgtgggatac gccaacccgg cgcaagtaaa gcggctattc aacaaggtaa aggcgcagat
     4201 cggcgacaag ctggcagggg ctcatttcca caacacgcgc ggcctgggcc ttgcgaatgc
     4261 gctcgcggcc tacgaagaag gcgtgcgcca cttcgacagc tcgatgggcg gccttggcgg
     4321 ctgcccgttc gcgccgggcg cgagcggcaa cgtgatcacc gaggatctgg tgttcatgtt
     4381 cgagagcatg ggcgtgccga ccggcatctc gctggagaag ctgttccagg cgcgggaaat
     4441 cctcgtcaag ggaattcccg aggagccgat ctacggccat acgccgctcg cagggctgcc
     4501 gaagggattc cgggccgccg cgtgagcgaa gccgcggttc aaaccgcaac tgccggcggc
     4561 gtgtgcacga tcacgctgaa ccggccggcg gccctgaacg cgctgaacca ggacctcgca
     4621 attggactgc gcgacgcggt tctcgccgcc gagcaggatc aatccgcccg ctgcctggtg
     4681 attcgtggcg gccagaactt catggccggc ggggacctca agtggttcga cgatctggtt
     4741 cgatcgcgcc gaccggagca gaaccggatc gagttcgagc gctttgccgg cgacgtccat
     4801 agcgtcatcg tcagcctgcg ccgcatgccg aagccggtgc tcgccagcgt gcgcggcgcc
     4861 gccgccgggt tcggcatgag cctgatgatg gcgtgcgatc tggtgatcgc ggccgacaac
     4921 gcctatttca gcctcgccta ttcgctcatc ggcgcaagcc ctgacggcgg ctcgaccttc
     4981 gcgctgccgc gcatcgtggg ggcgaagaag gcgatggaaa tcgctttgct ctcggagcgc
     5041 ttcgatgccg ctaccgccga gcggctcggc ctggtgaacc gcgtcgtgcc ggccgcggcc
     5101 ctggatgacg aaacggctag actcgccgcg cggctcgccg gcggcccgac ggcagtgtat
     5161 ggacgcacca agcagctgct gaacgcttcc ctcaactcga gcctcgagtc gcagctgcag
     5221 cgcgaagccg aggcgttcgc ccagagcgcc tccgagccgg cttttgcaga gggcgtgcgt
     5281 gctttcatcg agaagcgcaa gccactgtgg cctcgctgaa aggcaagacg ctcttcatca
     5341 ccggcggcag ccgcggcatc ggcctcgcga ttgcgctgcg cgcagcggcc gacggcgcga
     5401 acctggtgat cgcggcgaaa acagtcgagc cgcatccaaa gctccccgga acgatccata
     5461 ccgcagcccg cgagatcgaa gcggcgggcg gccggtgcct gccgttgcag tgcgacatcc
     5521 gcgacgaggc gcagctcgcg cgcgccgtag cggccgcggc cgagcgcttt ggcggcatcg
     5581 acatcctggt caacaacgcg agcgccatca gcctgagcgg cacgatggaa acgccgctca
     5641 agcgcctcga cctgatgttc gggatcaacg tgcgcgggac ctatgcgtcg tcgcaggcct
     5701 gcgtgccgca tctgcgcaag gcggcgaacc cgcacatcct gacgctctcg ccgccgctta
     5761 acatgaaccc gcgctggttc aagaaccacg tcgcctacac catggcgaaa tacggcatga
     5821 gcatgtgcgt gctcggcatg gcggaggaat ttcgtgctgc cgggatcgcg gtgaacgcct
     5881 tgtggccgaa gaccatcatt gccaccgccg cgctgcagat gattcccggc attgatccga
     5941 cgcgcgggcg caagcccgag atcatggccg atgccgcgca ctggatcctg acgcagcccg
     6001 cgcgcgcgtg cagcggcaat ttcttcatcg acgaggaagt gctgcgcaaa gccggcgtca
     6061 ccgatttgcg ccagtacgcc gtcgacccgt ccaaggacct gcttccagac ctgttcctcg
     6121 actgatgctg cccctcgaag gcattcgggt ggtggagttc gcccacatgg tgatgggccc
     6181 ctcctgcggt ctggtgctcg ccgatctcgg cgccgaggtg atcaaggtcg agccgctcgc
     6241 cggcgacaat acgcggcggc tggaggccgc gggcgcaggt ttcttcccgg tattcaaccg
     6301 caacaagaaa agcctcgcga tcgacctgca gaagaatgaa ggaaaacaga tcgtcaaacg
     6361 cctgctcgcg accgccgacg tgcttaccga gaacttccgt cccggcgcgc tcgacaaact
     6421 tggtttttca tttgattcat tgaaaacaat caacccgcgg ctgatttact gttcgctgaa
     6481 gggctttctc aaaggtccct acgagcatcg cctcgcgctc gacgaggtga cgcagatgat
     6541 gggtggcctc gcgtacatga ccgggctgcc cgagcggccg ctgcgtgccg gttcctcggt
     6601 ggtcgacatc atgggcggca cgttcgccgc ggtcggaatt ctggcggcgc tgcgcgagcg
     6661 cgagaagacc cggcgcggca cgcgcgtgac cagctcgctt ttcgagagca ccgcgtacct
     6721 cgtggcgcag cacatggcgc agttcgagct taccggcgaa gcgccgccac cgatgtcggt
     6781 caagcggcca gcctggggga tctacgacat cttcgacacc gccggcggcg g
//
LOCUS       DASTPD010000009         7147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_616375, whole genome shotgun sequence.
ACCESSION   DASTPD010000009 DASTPD010000000
VERSION     DASTPD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616375"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFR57_00300"
     CDS             <1..889
                     /locus_tag="VFR57_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257850.1"
                     /translation="GFGDLAYVGDTGGNVWRLDFTDTDPAKWSVRRVATLASSDPADI
                     ANKRKFLFSPEVAIAQDALGLYFAVLVGSGDREHPFDGIVVNRFYMLKDRGEADTLGS
                     LTGNRSYTQTTVTTTSTGGPAGKIIQEADLFDATNTVGASDSGWMRTLRPGEKIVSSA
                     IVVEGTAVFSSNQPTVLAGTAVDACASNLGVARVYTINVADGGGTSVIRAGGGYVPSP
                     VFAAIQLGSVSGGVSTIPPDSQGACTGAACGDQTIAGGGPGSGGPISGIICTGTSCWS
                     VGTIDVGSRRRSYWYKEVD"
     gene            886..1410
                     /locus_tag="VFR57_00305"
     CDS             886..1410
                     /locus_tag="VFR57_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0257851.1"
                     /translation="MSAGAARFGAPLAASLALHLVVLSAATGGVSDPVATRIPATLRV
                     QLQTPAPAPPPAQSDPAPVARASPKRYLTTSEVDERAVPLEMAPLLYPKTAYINRIRG
                     MVRVRVYISAAGRVDKAEVVEAAPKGHFEEAAIDAVQRTTFTAARKAGRAVPSQKLVE
                     VEFDPYGPTPEDRL"
     gene            complement(1380..2336)
                     /gene="ispH"
                     /locus_tag="VFR57_00310"
                     /gene_synonym="lytB"
     CDS             complement(1380..2336)
                     /gene="ispH"
                     /locus_tag="VFR57_00310"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU0257852.1"
                     /translation="MEVLLANPRGFCAGVERAIETVQRALELLGAPIYVRHEIVHNRY
                     VVDDLRAKGAVFVDELDEVPPGGTVIFSAHGVSKQVRADAERRGLKVFDATCPLVTKV
                     HVEVMRMLRAGHEVVMIGHRGHPETEGTMGQAANGMHLVETVADVARLDVANPERLAY
                     VTQTTLSIDDAKAIVAELKKRFPSIRGPKKDDICYATQNRQDAVKFMAPQCDVVIVVG
                     SPNSSNSNRLREVAQNMGAHAYMVDRAADLRPEWIADKRRVGVTAGASAPQVLVDELI
                     ARLKELGAQSVRQLDGITERVVFSLPRTLAQPDQSRSSGVGP"
     gene            complement(2341..2871)
                     /locus_tag="VFR57_00315"
     CDS             complement(2341..2871)
                     /locus_tag="VFR57_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0257853.1"
                     /translation="MKSELALLLKALAFAAHKHRDQRRKDPAASPYINHPIALADVLV
                     NEGGVTDVEVLCAALLHDTVEDTDTTPQEIEGAFGARVARIVAEVTDDQTLSKSERKR
                     LQVERAAGLSSEAKLVKLADKICNLRDVAERAPAGWDLARRREYFDWAKRVVDGLRGG
                     HAGLEAAFDAAYAMRK"
     gene            complement(2843..3319)
                     /gene="lspA"
                     /locus_tag="VFR57_00320"
     CDS             complement(2843..3319)
                     /gene="lspA"
                     /locus_tag="VFR57_00320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU0257854.1"
                     /translation="MSRWFALAAAVILLDQVTKWAVLENFVYGERREITGFFNLVLVY
                     NKGAAFSMFASADGWQTPLLILFAIVAAGIVSYLIVRNREKRVLCLGLALILGGALGN
                     LIDRLRFGHVVDFLDFHAMGWHWPAFNVADSGITVGALLLILDGFVHHEKRASAPS"
     gene            complement(3312..6059)
                     /gene="ileS"
                     /locus_tag="VFR57_00325"
     CDS             complement(3312..6059)
                     /gene="ileS"
                     /locus_tag="VFR57_00325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU0257855.1"
                     /translation="MADYKDTLNLPETPFPMRGDLAKREPQWVKEWDQKGVYRKLRAI
                     AKGRPRFVLHDGPPYANGDIHIGTAVNKILKDIIVKSKTLAGFDAPYVPGWDCHGMPI
                     EVRIEKKHGKNLPTAETLRLCRAFATEQIERQKKDFQRLGVLGDWDNPYLTMAPRTEA
                     EEIRVLGKLLDKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDPAIDVGFAFLEPEK
                     LARAFGAKVDKRGFAVIWTTTPWTLPANQALNVHPDLTYDLIDTERGLLILATELRDA
                     CLQRYGLSGTTIGQCKGRALENIRFRHPFYDRDAPVYLGEYVTLDAGTGVVHSAPAYG
                     VEDFESCRRYGMRDEEILTPVMADGRFAASLPLFGGLSIWDANSKIVKAIEEKGALLA
                     VDWKHTHSYMHCWRHKTPVILRATTQWFASMDKAVQGESLRATALRGIENTEFFPPWG
                     QARLHGMIANRPDWTLSRQRQWGVPMPFFLHRETGALHPRTAELLESVAKRVEKDGIE
                     AWQSFGGDAGPDYEKSRDTLDVWFDSGSTHYTVMRGSHASETRFPADLYLEGSDQHRG
                     WFHSSLLVSCMLDGIAPYKALLTHGFVVDGEGRKMSKSKGNVVAPQQVSNSLGAEILR
                     LWVASADYSGELFISNEILKRVVESYRRVRNTLRFLLANTSDFDAKRHAIGLSDMVEI
                     DRYAIAMTGAMQDQVITDYQRYQFHLVAQRLQAFCSEDLGAFYLDVLKDRLYTCGADS
                     KARRSAQTTLWHITHGLVRLMAPILSFTAEELWKVFTKEESVFFTTWHELPRSTDAAE
                     LLAKWGRLRELRDPVRKEIESLRAAGKVGSSLQAEVHFGANGENYEVLASLGDELKFL
                     LLTSAARLVKSDELEVRVSASANSKCERCWHYRADVNDEALCGRCQSNLRGPGEARRH
                     V"
     gene            complement(6052..6957)
                     /locus_tag="VFR57_00330"
     CDS             complement(6052..6957)
                     /locus_tag="VFR57_00330"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0257856.1"
                     /translation="MVVTHGSLQRAQGRCALTIGNFDGVHRGHRALIERMIAKAREAR
                     LASCVLTFEPHPREFFARDQAPARITRLRDKLALMAEAGVQRVHVARFDARFASLPAE
                     RFIDEVLVRGLGVHCLLVGRDFRFGARRTGDFSTLQNRGFTVEAMPDVQFDGVRVSSS
                     AVRAALQAGDISSAERLLGHPYAISGRVAHGAKLGRALGFATANIVLRRAPPVSGIFV
                     VDVDRYGPGVASVGKRPTVNPVPIPLLEVHLFDYGGADLYGEHLRVRFLKNLRDEQKY
                     DGLPALRDAIARDARQAREYLLTYG"
BASE COUNT         1246 a         2358 c         2293 g         1250 t
ORIGIN      
        1 cggctttggc gacctcgcct atgtgggcga caccggcggt aacgtgtggc gcctggattt
       61 caccgacacc gatcccgcca aatggagtgt gcgccgggtt gccacgctcg cttcgtccga
      121 tccggccgat atcgcgaaca agcgcaagtt tctcttctcg ccggaggtcg cgatcgcgca
      181 ggatgcgctt ggcctgtact tcgccgtgct cgtcggcagc ggcgaccgcg aacacccgtt
      241 cgacggcatc gtcgtcaacc gcttctacat gctgaaagac cggggcgagg ccgatacgct
      301 aggctccttg acggggaacc gctcgtatac gcagaccacg gtcacgacga cgagcaccgg
      361 cggcccggcc ggcaagatca tccaggaagc ggacctcttc gacgcgacga acaccgtcgg
      421 tgcgagcgac tccgggtgga tgcgcacgct gcgtcccggt gagaagatcg taagcagcgc
      481 aatcgtcgtc gagggcacgg cggtcttcag ctcgaaccag cccacggtgc tcgccggcac
      541 ggcggtcgac gcgtgcgcga gcaacctcgg cgtggcgcgc gtttacacca tcaacgtcgc
      601 ggacggcgga ggtacttcgg taatcagagc aggcggcggt tatgtgccct cgccggtgtt
      661 cgccgcgatc cagcttggct ccgtcagcgg cggcgtctcc acaatccctc cggactcgca
      721 aggcgcgtgc accggcgcgg cgtgcggcga ccagaccatt gccggcggtg gtccgggcag
      781 cggcgggccg atcagcggca tcatctgcac cggcacctcg tgctggtcgg tgggtacgat
      841 cgacgtcggc tcgcgcaggc ggtcctattg gtacaaggaa gtcgattgag cgcaggcgcg
      901 gcgcgcttcg gcgcgccgct cgccgcatcg ctcgcgctgc acctcgtggt gctgagcgcc
      961 gcgacggggg gcgtttctga cccggtagca acccgtatcc cagccaccct gcgggtgcag
     1021 ctgcaaacgc ctgcacccgc gccaccgccc gcgcagtccg accctgctcc agtggcgcgc
     1081 gcttcgccga agcgctacct cactacgagc gaggtcgatg agcgcgcggt ccctctggag
     1141 atggcgccgc tcctctatcc caagaccgcc tatatcaacc gcatccgggg aatggtgcgc
     1201 gtgcgcgtct atatctcggc tgcggggcgt gtggacaagg cagaggtcgt cgaggcggct
     1261 cccaagggcc atttcgaaga ggccgcgatc gatgccgtgc aacgcacgac attcacggct
     1321 gcgcgcaagg ccgggcgcgc cgtaccgagt cagaagctgg tcgaggtgga gttcgaccct
     1381 tacgggccga cgcccgagga ccggctttga tcgggctgcg cgagggtgcg cggcaagctg
     1441 aacaccacgc gctcggtaat gccgtcgagc tggcgcacgc tctgcgcacc gagctctttt
     1501 agccgcgcga tcagctcgtc caccagcacc tgaggcgcgg aagcgccggc ggtcacaccg
     1561 acgcggcgct tgtccgcgat ccactccggc cgcaggtcgg cggcgcggtc gaccatgtag
     1621 gcgtgcgcgc ccatgttttg cgccacttcg cgcagccggt tggaattcga gctgttcggc
     1681 gagcccacca cgatcaccac gtcgcactgc ggcgccatga acttcaccgc atcctggcgg
     1741 ttctgcgtgg cgtagcagat gtcgtccttc ttcggtccgc ggatggaggg aaagcgcttt
     1801 ttcagctcgg cgacgattgc cttcgcgtcg tcgatggaga gcgtggtctg tgtgacgtaa
     1861 gcaaggcgct cggggttggc cacgtcgagg cgagccacgt cggcgacggt ctcgacgaga
     1921 tgcatgccgt tcgccgcctg ccccatcgtg ccttcggtct ccgggtgccc gcgatggccg
     1981 atcatcacga cctcgtgccc cgcgcgcagc atgcgcatga cctcgacgtg gaccttggtc
     2041 accagcggac acgtggcgtc gaataccttg agcccgcggc gctcggcatc ggcgcgcacc
     2101 tgcttggata cgccgtgcgc gctgaagatg accgtgccgc cgggcggcac ctcgtcgagt
     2161 tcgtccacga acaccgcacc tttggcgcgc agatcgtcca ccacgtagcg gttgtgcacg
     2221 atctcgtggc gcacgtagat cggcgcgccg agaagctcga gggcgcgctg caccgtctcg
     2281 atggcgcgtt caacgccggc gcagaagccg cgcggattgg cgagcaagac ttccatcgcg
     2341 ctattttcgc atggcgtatg cggcgtcgaa ggccgcttcc agtccggcat gcccgccgcg
     2401 caggccgtcg accacccgct tcgcccaatc gaaatactcg cgccggcggg caagatccca
     2461 gcccgccggc gcgcgttctg cgacgtcgcg caggttgcag atcttgtcgg cgagcttgac
     2521 gagcttcgcc tcggacgaga gaccggcggc gcgctcgacc tgcaggcgct tgcgctcgct
     2581 cttgctgagg gtctgatcgt cggtcacctc ggcaacgatg cgcgccactc gcgcgccgaa
     2641 cgcaccctca atctcctgcg gcgtggtatc cgtgtcctcc accgtatcgt gtagcagcgc
     2701 cgcgcacagc acttcgacgt ccgtaacgcc gccctcgttc accagcacgt cggcgagagc
     2761 aatcggatga ttgatgtacg gcgatgccgc cggatccttg cgtcgctgat cgcgatgctt
     2821 gtgcgcggcg aaggcgagcg ccttaagaag gagcgctagc tcgcttttca tggtgcacga
     2881 agccgtccag gatgaggagc agcgcgccca ccgtgatgcc cgaatcggcc acgttgaacg
     2941 ccggccagtg ccatcccatc gcatggaaat cgaggaagtc gaccacgtgg ccgaagcgca
     3001 agcgatcgat caggttgccg agcgcgccgc ccaggatgag ggcgagcccg aggcacagca
     3061 ctcgcttctc ccggttgcgc acgatgagat agctcacgat ccccgccgcg acgatggcga
     3121 acaagatgag gagcggcgtc tgccagccgt ccgcgctggc aaacatgctg aacgccgcgc
     3181 ccttgttgta aaccagcacc agattgaaga acccggtgat ctcgcgccgt tctccgtaga
     3241 cgaagttctc gagcaccgcc cacttcgtga cctggtcgag caggatcacg gccgccgcca
     3301 gcgcgaacca cctagacatg gcgccgcgcc tcgcccgggc cgcgcaggtt cgactggcat
     3361 cgtccgcaca gcgcctcgtc gttcacgtcg gcgcggtagt gccagcagcg ctcgcacttc
     3421 gagttggcgc ttgcgctcac ccgcacttcc agctcgtcgc ttttcactaa tcgcgccgcg
     3481 gaagtgagaa gcagaaattt gagctcgtcg cccaagctgg cgagaacttc gtagttttcc
     3541 ccgttcgcgc caaaatggac ctcggcctgc agcgaggaac ccaccttacc cgctgcgcgc
     3601 aggctctcga tctccttgcg caccgggtcg cggagctcgc gcagccttcc ccacttggca
     3661 aggagctccg cggcatcggt ggaccgcggc agctcgtgcc atgtggtgaa gaagacgctc
     3721 tcttctttgg tgaacacttt ccacagctct tcggcggtga acgagaggat cggagccatc
     3781 agcctcacca ggccgtgcgt gatgtgccag agcgtggtct gcgccgagcg ccgcgccttg
     3841 gaatcggcgc cgcaggtgta gagccgatcc ttcagcacgt cgagatagaa agcgccgagg
     3901 tcttcggagc agaacgcctg aaggcgctgc gccaccaggt ggaactggta gcgctggtag
     3961 tccgtaatca cctggtcctg catcgcccct gtcatggcga tcgcgtagcg gtcgatctcg
     4021 accatgtcgc tcaagccgat cgcgtgccgc ttcgcatcga agtcggaggt attcgcgagg
     4081 agaaaacgca gcgtgttgcg cacgcgccgg tagctctcca ccacgcgctt gaggatctcg
     4141 ttcgaaatga agagctcgcc ggagtaatcg gcgctcgcca cccagaggcg caggatctcg
     4201 gcgccgaggc tgttcgatac ctgctgcggc gcgaccacgt tgcccttgga cttggacatt
     4261 ttccggcctt cgccgtcgac gacgaagcca tgcgtgagca gcgccttgta cggcgcaatg
     4321 ccgtcgagca tgcaagacac gaggagcgac gaatggaacc agccgcgatg ctggtcggaa
     4381 ccctcgagat acaagtcggc ggggaagcgc gtctcggaag catgcgagcc gcgcatgacc
     4441 gtgtagtgcg tggagccgga atcgaaccag acgtccagcg tatcccggct cttttcgtaa
     4501 tccggcccgg catcgccgcc gaagctctgc catgcctcga tgccgtcctt ctcaacgcgc
     4561 tttgcaacgc tctccagcag ctccgcagta cgcggatgca gagcgcccgt ctcgcggtgc
     4621 aggaagaacg gcattggcac gccccactgg cgctgccggg agagcgtcca gtccgggcga
     4681 ttcgcgatca tgccgtgcag ccgcgcctgt ccccatggcg gaaaaaactc ggtgttctca
     4741 attccgcgca gcgcggtggc gcgcaatgat tcgccctgca ccgctttgtc catcgacgcg
     4801 aaccactgcg tcgtcgcgcg caggatgacc ggcgtcttgt gccgccaaca atgcatgtag
     4861 ctgtgcgtgt gtttccagtc aactgcgagc agcgcgccct tttcctcgat cgctttgacg
     4921 atcttggaat tcgcgtccca aatactgagg ccaccgaaga gcgggagtga cgccgcgaag
     4981 cggccgtcgg ccattacggg cgtgaggatt tcctcgtcgc gcatgccgta gcggcggcag
     5041 gactcgaaat cctccacgcc gtaggcgggc gccgaatgaa cgacgccggt gccggcatcc
     5101 agcgtcacgt actcgccgag gtacaccggc gcgtcgcggt catagaaggg gtggcgaaag
     5161 cgaatgtttt cgagtgcccg ccccttgcac tgcccgatgg ttgtgccgga gagaccatag
     5221 cgttgcagac atgcgtctct tagttcagtt gcaaggatga gcagtccccg ctccgtgtcg
     5281 atcaggtcgt aagtcagatc cggatggacg ttcagcgcct ggtttgcggg cagcgtccaa
     5341 ggcgtcgtgg tccagatcac cgcgaaaccg cgcttgtcga cctttgcgcc gaaagcgcgg
     5401 gcaagctttt ccggttcgag gaaagcgaaa ccaacgtcga tggccggatc cttgcggtct
     5461 tcgtactcca cctcggcttc ggcgagcgcg gagccgcagt cgaagcacca gttcaccggc
     5521 ttcagaccac ggtaaacgta gcccttgtcg agcagcttgc cgagcacgcg gatctcttcg
     5581 gcttcggtgc gcggcgccat cgtcaggtag gggttgtccc aatcgccgag aacgccgagg
     5641 cgctggaagt ccttcttctg gcgctcgatc tgctcggtag cgaaggcgcg gcacagccgc
     5701 agcgtctcgg cggtcggcag gttcttgccg tgcttctttt cgatccgcac ctcgatcggc
     5761 atgccatgac agtcccatcc cggaacgtac ggcgcgtcga agccggcgag cgtcttcgac
     5821 ttcacgataa tgtccttaag aatcttgttg accgcggtgc cgatgtgaat atcgccgttc
     5881 gcgtacggcg ggccgtcgtg cagcacgaag cgcggccgcc cctttgcgat ggcgcgcagt
     5941 ttgcggtaga cgcccttctg gtcccactcc ttgacccatt gcggctcgcg cttggcgaga
     6001 tcgccgcgca tgggaaacgg cgtttcgggc aggttgagcg tgtctttgta atcagccata
     6061 ggtcaggagg tactcgcgcg cttgccgcgc gtcgcgcgca atagcgtcgc gcagcgcggg
     6121 cagtccgtcg tacttttgct cgtcgcgcag attcttcaag aagcgcacac gcagatgctc
     6181 accgtagagg tccgcccctc cgtagtcgaa aaggtgcacc tcgaggagcg ggatgggaac
     6241 cggattcacg gtcggccgct tgccaacgct cgccacgcca ggcccgtaac gatccacatc
     6301 caccacaaaa atccccgaga cgggaggcgc acggcgcagg acgatattgg cggttgcgaa
     6361 tccaagtgct cggcccagct tggcgccatg cgcgacgcgg ccgctgatcg cgtagggatg
     6421 gccgagcagg cgctcagctg aagagatgtc gcccgcctgc aacgcagcgc ggaccgccga
     6481 gctcgaaacg cgcacgccgt cgaactggac gtcgggcatc gcttcgaccg tgaacccgcg
     6541 attctgcagt gtggaaaagt ctccagtgcg gcgcgccccg aagcgaaaat cgcgtccgac
     6601 cagaaggcag tgcacgccga ggccgcgcac cagcacctcg tcgatgaaac gctcggccgg
     6661 aagcgacgca aagcgcgcgt cgaagcgggc cacatggacg cgctgcacgc cggcctctgc
     6721 catcagcgcc agcttgtcgc gcagccgcgt gatgcgcgcc ggcgcctggt cgcgggcgaa
     6781 aaattctctt ggatggggct caaaggtgag aacacaggag gcgagcctgg cttcgcgcgc
     6841 cttcgcgatc atcctctcga tgagcgcgcg atggccgcgg tgcacgccgt cgaagtttcc
     6901 gatcgtcagc gcacaccggc cctgggcgcg ctgcaaagag ccgtgcgtga cgaccatgga
     6961 ctcggttaaa acattgaatt atgccgcaga gttgccggaa atccctgttt ccatagagaa
     7021 accatgcttt acggttcccc atcaatagcg tacgggatag cctagaaaac actacgcatg
     7081 gggaattacg gtccccttgc aaccgttcac gggtggcaat aatcagtagt acgtaaatac
     7141 cgcccca
//
LOCUS       DASTPD010000010         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1228939, whole genome shotgun sequence.
ACCESSION   DASTPD010000010 DASTPD010000000
VERSION     DASTPD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228939"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1428)
                     /locus_tag="VFR57_00335"
     CDS             complement(166..1428)
                     /locus_tag="VFR57_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0257857.1"
                     /translation="MSRASRPSFVIILADDLGYADLGCYGGRGNCSPNLDRIAAEGLR
                     FTDGYSNSPVCSPTRFALATGRWQYRLRGGADEPIASRSRGSEILGLPPAHPTLASLL
                     RDAGYATALVGKWHLGFPPRFGPLKSGYQEFFGPMSGAVDYFTHCDPAGVHDLWQGEK
                     EVHRSGYLTDLLSDEAIAFVRGAKGRPFLLSLHYTAPHWPWETRADAEESKRISSISH
                     IDGGSVAIYLEMIRAMDDGIGRLMRELDDNTIVVFTSDNGGERFSDNWPLVGGKMDLL
                     EGGIRVPYIVRWPKNGIIPQLAITMDWVATFLDVADLAPHPDHPLDGVSLLQEHSTRE
                     LFWRMKFRSQKAMRAGSWKWLSIEGNEFLFDLGKDQRERANLGRREPGKLEEMRSHYA
                     AWEATLPPIPDDARVSLVYGPSDIPKPS"
     gene            complement(1412..2362)
                     /locus_tag="VFR57_00340"
     CDS             complement(1412..2362)
                     /locus_tag="VFR57_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0257858.1"
                     /translation="MKRLALLTLAFVTLQAAAQVYPSKPVRMVNPFPPGGPLDVLGRI
                     LSERLGASLGRPVLVENRPGAAGNVGAAEVAKSASDGHTLLLTLFSIVTSNPHLYERL
                     AFDPLKDFAPITALASSDSVLIAHPSVPAGSPQELIAYAKANPGRLNYASAGKASPGH
                     LTAEFFTRRTGATFTHVPYKGNAEAIRSVLAGETQMMFTPTTGALPHIKSGKLKALAV
                     YLTERVDELPGVPALDGFDQKTLPFWYGLLAPAGTPREIVLRLNAETVKALREPQVET
                     TLRAGRIFPIGNTPEEFGAMMREGHAAWGRVIRETGVKGE"
     gene            complement(2367..2816)
                     /locus_tag="VFR57_00345"
     CDS             complement(2367..2816)
                     /locus_tag="VFR57_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257859.1"
                     /translation="MKAFLLILILAVPGLAFGASDPVTPEARDPVLDKVSEATKRQDW
                     ASAATVLKDAVASNPGNANYHNLYAYSIRKGTNPDMNLVFKHYNEALRIDPKHRGAHE
                     YIGEAYLMVGNVDKAKEHLGVLDKLCFFPCSEYTDLKKAIATYEAKR"
     gene            2839..3480
                     /locus_tag="VFR57_00350"
     CDS             2839..3480
                     /locus_tag="VFR57_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0257860.1"
                     /translation="MVLLAVALTAAAPRAFPQAPQTRAEALGALASPDAATRAEAVVW
                     LANRGGMEDALLLHERLRDESSFVRGFAEQGLWLLWSRSGNAEIDRLMALGSQAMQAG
                     RHSEAIGVFSRIIETKPEFAEGWNRRATVYYLAGQYDKSIADCHEVLKRNPRHFGALS
                     GMGQIHVQLEDYENALRWYRRALEVNPNMLGVEMNIRLLEEKLKDQRAPGRST"
BASE COUNT          617 a         1139 c         1207 g          617 t
ORIGIN      
        1 gccggcttcg tgcccccgga agtcgaggcg ctgaacgaga tcgcggactt ggagcgcctg
       61 gtgacgaagg actcgaaggc ggcacgcaag cttgccctcc tcaagacgcg tatcgagacc
      121 cggtactacg aaagaacgct cgccaagctt ggccgctagt agcgctcagg acggcttggg
      181 aatgtcggac ggaccgtaga ccagcgacac ccgcgcgtcg tccgggatcg gcggcagcgt
      241 tgcttcccac gccgcgtagt ggctgcgcat ttcctccagt ttccccggct ccctcctgcc
      301 aagatttgcg cgctcgcgct gatccttccc caggtcgaac aggaattcgt tgccttcgat
      361 cgagagccac ttccagcttc ccgcgcgcat cgccttctgg ctgcgaaact tcatccgcca
      421 gaagagctcg cgcgtcgaat gctcctggag gaggctgacg ccgtcgagcg ggtggtcggg
      481 atgcggcgca aggtcggcca catcgaggaa agtcgcgacc caatccatcg tgatcgcaag
      541 ctgaggaatg atgccgtttt tcggccagcg cacgatgtac ggcacccgga taccgccttc
      601 gaggaggtcc attttcccgc cgacgagcgg ccagttgtcc gagaagcgct cgccgccgtt
      661 gtcgctggtg aacaccacga tcgtgttgtc gtcgagctcg cgcatcaggc ggccgatgcc
      721 gtcgtccatt gcccggatca tctccaggta gatggctacc gagccgccgt cgatgtggct
      781 gatgctcgag atcctcttcg actcctcagc atcggcacgc gtctcccagg gccagtgggg
      841 ggcggtgtag tgcagcgaga gcaggaacgg ccttcccttc gccccgcgca cgaacgcaat
      901 ggcctcatcg ctgagcaggt cggtgagata gccgctgcgg tggacttcct tctcgccttg
      961 ccagaggtcg tgcaccccag cgggatcgca atgcgtgaag tagtcgaccg cgccgctcat
     1021 cgggccgaag aactcctggt agccgctctt caacgggcca aagcgcggcg gaaagcccag
     1081 gtgccacttg ccgacgagcg ccgtggcgta gccggcatcc cgaagcaagg aagcgagcgt
     1141 cgggtgtgcc gggggtaacc ccaaaatttc actcccacgc gagcggctcg cgatcggctc
     1201 gtccgcaccg ccccgcagcc ggtattgcca gcggccggtg gcaagcgcga agcgcgtcgg
     1261 cgagcagacc ggcgaattgg aatagccgtc ggtgaagcgc aaaccttcgg cggcgatacg
     1321 gtcgagattc ggagagcagt tgccgcggcc gccgtagcag ccgaggtcgg catagccgag
     1381 gtcgtcggca aggatgatga cgaagctcgg cctactcgcc cttgacaccg gtctcgcgga
     1441 tcacgcggcc ccaggcggca tggccctcgc gcatcatcgc gccgaattcc tcgggcgtat
     1501 taccgattgg gaagatgcgt cctgcgcgca gggtggtctc cacctgaggc tcccgtagcg
     1561 ccttgaccgt ctcggcattg aggcgcagga cgatctcgcg cggagtgccg gccggcgcga
     1621 gcagcccgta ccagaacggc agcgtcttct ggtcaaagcc gtcgagtgca ggcacgcccg
     1681 gcagctcgtc tacgcgctcg gtgaggtaca ccgcgagcgc cttgagcttg ccgctcttga
     1741 tgtgcggcag cgcccccgtc gtaggtgtga acatcatctg ggtctcgccc gcgaggacgc
     1801 tgcggatcgc ttcggcgttt cccttgtatg gcacgtgcgt aaacgttgcg ccggtgcggc
     1861 gggtaaaaaa ttcggcggtg aggtggcccg gcgatgcctt gccagccgag gcgtaattga
     1921 gcctgccggg gttcgctttg gcgtaagcga tcagctcctg cggcgagccg gccggcaccg
     1981 aagggtgggc gatgagcacc gagtcgctgc tcgccaacgc ggtgatcggc gcgaagtcct
     2041 tcagcgggtc gaacgccagc cgctcataga ggtgcggatt gctcgtgacg atgctgaaaa
     2101 gggtgaggag cagcgtgtgc ccgtcgctcg ccgacttggc tacctcggcg gcgcccacgt
     2161 tgccggccgc gcccggccgg ttttccacca gcaccggccg cccgaggctc gcgccgaggc
     2221 gctcggagag gatgcggccg aggacgtcga gcggcccgcc cggcggaaac gggttcacca
     2281 tgcgcaccgg tttcgacgga tagacctgcg ccgccgcctg cagcgttaca aacgcgagag
     2341 tgaggagcgc gagacgcttc aagcgctcag cgcttggctt cgtacgtcgc gatcgccttc
     2401 ttgagatcgg tgtactccga gcacgggaag aagcaaagct tgtcgagaac gccgaggtgc
     2461 tccttcgcct tgtccacgtt accgaccatg agatacgcct cgccgatgta ctcgtgcgcg
     2521 ccgcggtgct tcgggtcgat gcgcagcgcc tcgttgtagt gcttgaacac gaggttcatg
     2581 tccgggttcg tacccttgcg gatcgagtag gcgtacaggt tgtggtagtt ggcattcccc
     2641 gggttcgaag cgacggcgtc cttcagcacc gtcgctgcgc tcgcccaatc ctggcgcttg
     2701 gtggcttcgg acactttgtc gagcacaggg tcgcgggcct cgggggtcac cggatcgctc
     2761 gccccaaagg caaggcctgg aacggctagt attagtatca acaggaaggc tttcatggtt
     2821 tctccttcaa ggcttgcaat ggtactcctc gcggttgcgc taacagcggc tgcgccgcgc
     2881 gcattcccgc aggcgccgca gactcgcgcc gaggcgcttg gcgcgctcgc gagcccggac
     2941 gccgcgacgc gcgcagaggc ggtagtgtgg ctggcgaacc gcggcggcat ggaagacgcg
     3001 ctgctgctgc acgaacggct gcgcgacgag agctcgttcg tgcgcggatt cgccgagcag
     3061 gggctttggc tcctgtggag ccgctcgggc aatgcggaga tcgaccgcct gatggcgctc
     3121 ggcagtcagg ccatgcaggc gggacggcac agcgaagcga tcggggtttt ttcgagaatt
     3181 atcgaaacaa agcctgagtt cgcggaaggc tggaaccggc gcgccaccgt ctattacctc
     3241 gccggccaat atgacaagtc gattgccgac tgccacgagg tattgaagcg caacccgcgc
     3301 cacttcggcg cgctctctgg aatggggcag atccacgtgc agctcgagga ctacgagaac
     3361 gcgctgcgct ggtaccgccg cgcgctggaa gtgaacccga acatgctggg cgtcgagatg
     3421 aacatcaggc tgctcgagga gaagctgaaa gatcagcgag cgcctgggcg atcgacctag
     3481 ccgcctcgcg cggatgctcg aacggaaaga ggtgaccgcc gggcaccttg cggaagctga
     3541 acttcggccg catgcccgcg agccggatgc gccgcacgac
//
LOCUS       DASTPD010000011         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_208562, whole genome shotgun sequence.
ACCESSION   DASTPD010000011 DASTPD010000000
VERSION     DASTPD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208562"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VFR57_00355"
     CDS             complement(<1..1122)
                     /locus_tag="VFR57_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU0257861.1"
                     /translation="MASVSPQSPAAAAGESFAALFEESLNRKEMRVGEVITAEVVSVD
                     ENFVVVNAGLKSESVIALDEFRNDAGQVEVKPGDFVSVSIEALEDGYGATRLSRDKAK
                     RMAAWLDLEKALESGEKVQGVITGKVKGGLTVMAKGIRAFLPGSLVDLRPVKDTTPYE
                     GKTMEFKVIKLDRKRNNVVVSRRAVLEETAGAEREALLASLQEGATVKGIVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVKHPSEVLNVGDEVTARVLKFDSEKNRVSLGLKQLGE
                     DPWVGIARRYPQATRLFGKVTNLTDYGAFVEVESGIEGLVHVSEMDWTNKNVHPSKVV
                     QVGDEVEVMILEIDEERRRISLGMKQCMPNPWEDFAREYK"
     gene            complement(1190..1855)
                     /gene="cmk"
                     /locus_tag="VFR57_00360"
     CDS             complement(1190..1855)
                     /gene="cmk"
                     /locus_tag="VFR57_00360"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU0257862.1"
                     /translation="MSVPVIAIDGPSASGKGTIAKRVAAAIGFHYLESGALYRLVALI
                     ALRQGLNDEAAIAAAAAGMDVYFQGEEIFLEDQDVNLHIRHEEVGKRASDIAPMPAVR
                     RALLERQRAFRQPPGLVADGRDMGTVVFPDAALKIFLTASPEVRAQRRYKQLIEKGIA
                     ANLSALSRDLAQRDARDANRAVAPLAPAPDSQVLDSSSLSVEAVADRVLQLWKGKITG
                     ERR"
     gene            complement(1852..3120)
                     /gene="aroA"
                     /locus_tag="VFR57_00365"
     CDS             complement(1852..3120)
                     /gene="aroA"
                     /locus_tag="VFR57_00365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0257863.1"
                     /translation="MDSLELKPARRAAGTVRLPGSKSISNRVLLLAALARGETEVLGL
                     LDADDTRVMREALSLLGVSFKTNTVKGVGGPFPVKKAELFLGNAGTAFRPLTAALALS
                     NGEYRLSGVARMHERPIGDLVDALRGIGARIDYAGKEGFPPLVVHAARLNTENVKVRG
                     NVSSQFLSALLMALPLAGRDSGIEVQGDLISKPYVEITLNVMQRFGISVKRDAWRAFH
                     VPARPYTSPGKIFVEGDASSASYFLAAGALGGGPVRVQGLGRGSVQGDVRFTEVLERM
                     GASVSLVDDAIEVKNDSRKLKPIDMDMNHIPDAAMTAAVLALFADGPSTIRNVASWRV
                     KETDRLAAMATELRKLGAQVEEGPDFLQIAPPSTLKGNVLVDTYDDHRMAMSFSLVAL
                     GGVPVRINDPQCVAKTFPGFFDSWVSILSS"
     gene            complement(3102..3944)
                     /locus_tag="VFR57_00370"
     CDS             complement(3102..3944)
                     /locus_tag="VFR57_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU0257864.1"
                     /translation="MRLAVIGVGLIGGSFALALKQAKAVSHVVGVGRNPANLKAALER
                     GIIDSIASDVAAAARDADLVLVATPVGQYPAVFGSLRDTKALITDGGSTKRDVIAAAR
                     KALGKGVSRFVPAHPIAGAEKSGAAAASAELFRDKRVVLTPLEENSKDSISRIEQAWK
                     ACGANVSRMSAQEHDAVLATVSHLPHLLAYALVHEVAGRENSAQLFSFAAGGFRDFTR
                     IASSHPEMWRDICIANRDRLLEELAKFSVKLQEIRNLLDNPTELEKLFAEARAARDKW
                     IRSS"
     gene            complement(3954..>4078)
                     /locus_tag="VFR57_00375"
     CDS             complement(3954..>4078)
                     /locus_tag="VFR57_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0257865.1"
                     /translation="GVIVRPVGGGYGLPEHLRVTIGTARENDRFLNALTVSLRE"
BASE COUNT          703 a         1374 c         1258 g          743 t
ORIGIN      
        1 cttgtactcg cgggcgaaat cttcccaggg gttcggcatg cactgcttca tgccgagcga
       61 gatgcggcgg cgctcctcgt cgatctccag aatcatcact tccacttcgt cgccgacctg
      121 caccaccttc gacggatgca cattcttgtt ggtccagtcc atctccgaaa cgtgcaccaa
      181 cccctcgatg cccgactcca cctcgacgaa cgccccgtag tcggtgagat tggtgacctt
      241 gccgaagagt cgggtcgctt gcggatagcg gcgggcaatg cccacccacg ggtcctcgcc
      301 gagctgcttg agcccgagcg agacgcggtt cttctcggag tcgaacttga ggaccctcgc
      361 cgtgacttcg tcgccgacgt tgagcacttc ggagggatgc ttcactcggc gccacgcgag
      421 gtcggtgatg tggagcagcc cgtcgattcc gccaagatcc acgaacgcgc cgtaatcggt
      481 gatgttcttg acgatgccct ttaccgtcgc gccttcctgc agcgaggcga ggagcgcttc
      541 gcgttccgcg cccgcggtct cctcgagcac cgcacggcgc gacacgacca cgttgtttcg
      601 cttgcggtcg agcttgatga ccttgaactc catcgtcttg ccttcgtacg gcgtggtgtc
      661 cttcaccggg cgcaggtcga cgagcgagcc cggcaggaac gcccggatgc ccttcgccat
      721 gacggtcagg ccgcctttca ccttgccggt aatcacgccc tggaccttct cgcccgactc
      781 gagcgccttt tccagatcta gccacgccgc catgcgcttc gccttgtcgc gcgacaggcg
      841 cgtcgcgccg tagccgtcct cgagcgcctc gatcgacacg ctcacgaagt cgccgggctt
      901 cacctcgacc tgccctgcgt cgttcctgaa ctcgtcgagc gcgatgacgc tctcggactt
      961 cagccccgca ttcaccacca cgaagttctc gtcgacgctc accacttcgg cggtgatcac
     1021 ttcgccgacc cgcatctcct tgcggttgag gctctcctcg aaaagcgccg cgaaggactc
     1081 tccggcggcg gctgcaggcg actgcgggga aacactggcc atgtgatgaa aaaacctttt
     1141 caaaccggag gcttggcccc cgggcgaagt tgaaaaccga gcaccgctcc tagcggcgct
     1201 cacccgttat cttgcccttc cacaactgca ataccctgtc ggcgaccgcc tcgaccgaaa
     1261 gactcgaaga atcgagtact tgcgaatcgg gcgccggtgc gagcggcgcc acggcgcggt
     1321 ttgcgtcgcg cgcatcgcgc tgcgcgaggt cccgcgaaag ggcgctcaga ttagcagcga
     1381 ttcctttttc tatcaactgc ttatatctgc gttgagccct tacttcgggg ctcgctgtca
     1441 agaagatctt gagcgccgcg tcggggaaga cgacggtccc catgtcccgg ccgtcggcca
     1501 ccagcccggg cggctggcgg aaagcccgtt gccgctccag gagcgcgcgg cgtaccgccg
     1561 gcataggtgc tatgtcggac gctcgcttgc cgacctcctc atgccggatg tgaaggttaa
     1621 cgtcttggtc ttcaaggaaa atctcttccc cctggaaata cacatccatc cccgccgccg
     1681 ctgccgcgat cgccgcctca tcgttaagcc cctgtcgcag tgcaataagc gccaccagcc
     1741 ggtacagcgc tccgctctcc aagtagtgga accctatcgc cgccgccacc cgcttcgcga
     1801 tcgttccttt cccggacgcc gaggggccgt cgatcgcgat caccgggacg ctcatgaact
     1861 gaggatgctc acccaggaat caaaaaaacc ggggaacgtc ttcgcgacgc actgcggatc
     1921 attgatccga accggcactc cgccgagcgc gacgagcgag aagctcatcg ccatgcggtg
     1981 atcgtcatag gtgtccacaa ggacatttcc cttgagggtc gatggcggcg cgatctgcag
     2041 gaaatccgga ccttcctcca cttgggcgcc aagcttgcgc aattcggtcg ccatcgcggc
     2101 gagacggtcg gtctccttca cccgccagct ggcgacgttg cggatcgtgc tcggcccgtc
     2161 ggcgaagagc gccagcaccg cagcggtcat cgcggcgtcg ggaatgtggt tcatgtccat
     2221 gtcgatcggc tttagcttgc gactgtcgtt cttcacttca atggcatcgt ctacgaggga
     2281 taccgaggcg cccatgcgct ccagcacctc ggtaaagcgg acatcgccct gaacgctgcc
     2341 tctcccgagg ccttgtacgc gcaccggccc gccgccgagc gcgccggcgg cgaggaagta
     2401 ggaagcggag gaagcgtcgc cttccacgaa gatctttccc gggctcgtgt acggccgcgc
     2461 cggcacatga aatgcgcgcc acgcgtctct cttcaccgaa atgccgaaac gctgcatcac
     2521 gttgagagtg atctcgacat agggcttgga gatcaggtcg ccttgcacct cgataccgga
     2581 atcgcggccc gccagcggca acgccataag gagcgcgctg aggaactgcg acgaaacgtt
     2641 tccccgcact ttcacgtttt cagtgttcag tcttgcagca tggacgacca ggggcgggaa
     2701 accctccttg cccgcgtaat cgatgcgcgc gccgataccg cgcagcgcgt cgacgagatc
     2761 gccgatcggc cgctcgtgca tgcgcgccac gccggaaagc cggtactcgc cgttggacag
     2821 cgcgagcgcg gcggtgagcg gtcggaacgc cgtgcccgca ttgcccagaa agagctcggc
     2881 tttcttcaca gggaacggcc cccccacgcc tttcacggtg ttggtcttga atgaaacacc
     2941 gagaagagag agcgcttcgc gcatcacgcg cgtatcgtcg gcgtcgagca ggccgaggac
     3001 ctcggtctcg ccgcgtgcga gcgcggcaag caggaggaca cggttggaga tgctcttgga
     3061 cccggggagc cgcaccgtgc cggcggcgcg gcgcgccggc ttcagctcga gcgaatccat
     3121 ttgtcgcgcg cagcgcgcgc ttcggcgaac agcttctcga gctcggtcgg gttgtcgagc
     3181 aggttacgga tttcctgcaa tttgacggag aactttgcaa gctcctcaag cagccggtcc
     3241 cggttggcga tgcagatgtc gcgccacatc tcggggtggc tggaagcgat gcgggtgaag
     3301 tcgcggaacc cacccgcggc aaacgagaag agctgcgccg agttctcgcg cccggcgacc
     3361 tcgtgcacca gcgcgtaggc gagaagatgc ggcaggtgac tcacggtggc gagtaccgca
     3421 tcgtgctcct gggcgctcat gcggctgacg ttggcaccgc acgctttcca cgcttgttcg
     3481 attctcgaaa tcgagtcctt cgagttctct tcgagaggcg tcaagaccac ccgcttgtcg
     3541 cgaaagagct cggcgctagc agccgccgcg ccgctttttt cggcgccggc gatcgggtgc
     3601 gcgggcacga atcgcgacac gcccttgccg agcgccttgc gagccgcggc gatgacgtcg
     3661 cgcttggtgc tgccgccgtc ggtgatgaga gccttcgtgt cgcgcagcga gccgaacacc
     3721 gccgggtact gcccgaccgg cgtcgccacc aggacaagat cggcgtcgcg cgctgcggcg
     3781 gcgacatccg acgcgatgga atcgatgatg ccgcgctcga gcgcggcctt gagattggca
     3841 gggttgcggc cgacgccgac gacgtgcgag acggcttttg cctgcttcag cgccagagcg
     3901 aacgaaccgc cgatcaggcc gacgccgatg acggcgagtc tcattgcctc atttcattcc
     3961 cgcagggaga cggtgagcgc gttaaggaag cgatcgtttt cccgagcggt cccgatcgtg
     4021 acgcgcaggt gctcgggcaa accgtagccg ccgccgaccg ggcgcacgat gaccccgc
//
LOCUS       DASTPD010000012         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1229314, whole genome shotgun sequence.
ACCESSION   DASTPD010000012 DASTPD010000000
VERSION     DASTPD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229314"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /gene="lpxC"
                     /locus_tag="VFR57_00380"
     CDS             <1..730
                     /gene="lpxC"
                     /locus_tag="VFR57_00380"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU0257866.1"
                     /translation="ASTLIKDGVKIHTVEHLMSALAGLGIDNVFVDLDAPELPIMDGS
                     ASPFALLLQQAGIEEQAAPKRFLRVKKSVEVKDGDKWARLEPHAGFKVSFSIDFRHPV
                     IERSTQSVMVDFAETSYLKEIARARTFGFMQDVEDLKDSGLALGGGLDNAVVLDEQGV
                     LNSEGLRFADEFIRHKLLDAIGDLYLLGRPLLGAFTAHKSGHALNNRLVRAALAETGA
                     LETVVFERAEEAPAGLARVSTQLG"
     gene            731..913
                     /locus_tag="VFR57_00385"
     CDS             731..913
                     /locus_tag="VFR57_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257867.1"
                     /translation="MFAVLRVAVVVVAVVLGFAVLAWLLTGDPRWRRIAWLVFKYAVF
                     ARAAILLLFAAEALVH"
     gene            1378..2316
                     /locus_tag="VFR57_00390"
     CDS             1378..2316
                     /locus_tag="VFR57_00390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0257868.1"
                     /translation="MQIILISDRLARARSVHLSVAHVAGTAFLALLLLLGATATLHWV
                     TLRYAAEMRLPVLQELLAAAQQAQAEREHVFVQQNLNSMAMKLGEMQAQLTRMDALGE
                     RLSALAGIRPQEFRIGEAPGLGGAAPTQMPPQNLSLDEFSDKLQVLARQVEGRNDMLG
                     VLEAQLFEQAVKKKMIPTMMPVKASYNPSGFGKRIDPFNGQWAMHEGIDFVSDYGSPI
                     VAAAAGVVVFSGFHPQYGYVVDIDHGNDLVTRYAHCSKLLVREGDLVQRGRKIAEVGS
                     TGRSTGPHLHFEVRFRGAAQNPAKFLVTSRQVAAVK"
     gene            2401..>3650
                     /locus_tag="VFR57_00395"
     CDS             2401..>3650
                     /locus_tag="VFR57_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0257869.1"
                     /translation="MPARIFGSRNQRLLKQYAHVVQAINALEPALEKLSDAQLRDKTT
                     ELRERHARGASLEELLPEAFAVVREASKRTLRMRHFDVQLLGGMVLHNGKIAEMKTGE
                     GKTLVATLPAYLNALTGKGVHIVTVNDYLANRDAEWMGKIYRFLGLTVGVNLPHMEPE
                     KKRAAYAADITYGTNNEFGFDYLRDNMALHVEERFQRGLHYAIVDEVDSILIDEARTP
                     LIISGQAEDRTEIYLRMNEVAPLLTRQKEEKKPDQPEPPGDFYVDEKNHQILLSEAGH
                     QKAEEILTRKGLLPEGASLYEPAYINLVHQLYAALRAHYLFHRDQHYVVQNDEVIIVD
                     EFTGRLMVGRRWSDGLHQAVEAKEKVSIQNENQTLASITFQNYFRMYGKLAGMTGTAD
                     TEAYEFQQIYGLETVVVPTHMKMIR"
BASE COUNT          647 a         1196 c         1204 g          603 t
ORIGIN      
        1 cgcctccacg ctgatcaagg acggcgtgaa gatccatacg gtcgaacatc tcatgtcggc
       61 gctcgcgggc ctcggcatcg acaacgtgtt cgtcgatctc gacgcgcccg agctgccgat
      121 catggatggc agcgcctcgc cgtttgcgct gctcctgcag caggcgggga tcgaggagca
      181 ggcggccccg aagcgcttcc tgcgcgtgaa gaagtcggtc gaggtgaagg acggcgacaa
      241 atgggcgcgc ctcgagccgc atgcaggctt caaggtttcg ttctcgatcg actttcgcca
      301 tcctgtgatc gagcgcagca cgcagtcggt catggtggac ttcgccgaga cctcgtacct
      361 caaggaaatc gcgcgcgcgc gcacgttcgg cttcatgcag gacgtggaag acctcaagga
      421 cagcggactc gcgctcggcg gcgggctcga caacgccgtg gtgctcgacg agcaaggcgt
      481 gctcaacagc gaggggctgc gcttcgccga cgagttcatt cgccacaagc tgctcgatgc
      541 catcggcgat ctctatctgc tcggccgtcc gctcctcggt gctttcaccg cgcacaaatc
      601 ggggcacgcg ctcaataacc gcctggtccg tgcggcgctc gccgagaccg gcgcgctcga
      661 gaccgtggtg ttcgagcgcg cggaggaagc tccggcgggc ctcgctcgcg tttccaccca
      721 gctcggctag gtgttcgcag tcctgcgggt cgcggtcgtc gtggtcgcgg tggtgctcgg
      781 gttcgccgtg ctcgcgtggc tgctcaccgg cgacccgcgc tggcggcgca tcgcctggct
      841 cgtgtttaag tacgcggttt tcgcgcgggc ggcgatcctg ctgctctttg ccgccgaggc
      901 tctggtccac tagggggcat cgcgcggccg cgccgctcga gcagcacgcg aagcgcctcg
      961 cgcgccggcg agctgcgcat cgagccatag agcctgctca agctgtcgag cgcgggcgtg
     1021 gacaaatggg cacgtttttg cggtgcaaca acgctcttgc gtgacacgtt tggttgcacc
     1081 ctggtggaaa ctgaactaac ctgcaagcgc tgttttgaaa agaattttgc cagggacggg
     1141 gcgagcagcc ggagctttgc cgccgcggct ggagacgccg ccagaagcat caactggccg
     1201 tcccggtaac tgacaactcg ggtctgtcgt gccagctcgg acggcaggaa cccgtcaagc
     1261 agcccggcga gcgcacgcag ctcgtgcgcc tttgcgagga ccggctgcag cgaggcttcg
     1321 gctgcgagga tccggtcgag cttgagcgga cgcacaaatg gcagggaggg agtgatcgtg
     1381 cagattatcc tgatctcgga ccggctggcg agggcgagat cagttcacct gtcggtcgcg
     1441 cacgtggcgg ggacggcgtt tctcgcgctg ctcctcttgc tcggggccac cgcaacgctt
     1501 cattgggtga cgctgcgcta cgctgccgag atgcgcctgc cggtgctgca ggagctgctc
     1561 gccgccgcgc agcaggctca agccgagcgc gagcacgttt tcgtgcagca gaacctcaac
     1621 tcgatggcca tgaagctcgg cgagatgcag gcgcagctca cgcgcatgga cgcgctgggc
     1681 gagcgcctgt cggccctggc cggcatccgg ccgcaggagt tccgcatcgg cgaggctccc
     1741 gggctgggcg gtgcagcgcc cacgcagatg ccgccgcaga atctctcgct cgacgaattc
     1801 agcgacaagc tgcaagtgct ggcgcgccag gtggaaggcc gtaacgacat gctcggggtg
     1861 ctggaagcgc agctctttga gcaggcggtc aagaagaaga tgatcccgac catgatgccg
     1921 gtgaaggcgt cctacaaccc gtcgggcttc ggcaaacgca tcgacccctt caacggccag
     1981 tgggcgatgc acgaaggcat cgatttcgtc tccgactacg gctcgccgat cgtcgccgcc
     2041 gcggccgggg tggtcgtctt ctccggattt cacccgcagt acgggtacgt ggtggatatc
     2101 gaccacggca acgacctcgt cacgcgctac gcccattgct cgaagctctt ggtacgcgag
     2161 ggcgacctcg tgcagcgcgg gcgcaagatc gccgaggtgg gttccaccgg ccgctcgacc
     2221 gggccgcacc tgcatttcga agtgcgtttc cgcggcgcgg cgcagaaccc ggccaagttc
     2281 ctcgtcactt cgaggcaggt cgccgccgtc aagtaaacgc agggttttcc agtaaaatcg
     2341 gcggtttcgc accgcccatt cgtggaattc ctgcgctcga tcctagaggc cctctggcgc
     2401 ctgccggcgc gcatcttcgg cagccgcaac cagcggcttc tcaagcagta cgcgcatgtc
     2461 gtgcaagcca tcaacgcgct cgagccggcg ctcgagaagc tttccgacgc gcagctgcgc
     2521 gataaaacga ccgagctgag agagcgccat gcccgcggcg cctcgctgga ggagctgctg
     2581 cccgaggcct tcgccgtggt gcgcgaagcg tccaagcgga cgctgcgcat gcggcacttc
     2641 gacgtgcagc tcctcggcgg catggtgctg cataacggca agatcgccga gatgaagacc
     2701 ggcgagggca agacgctcgt cgccacgttg ccggcgtacc tgaatgcgct cactggcaag
     2761 ggggtgcaca tcgtcacggt gaacgactac ctcgccaacc gcgacgccga gtggatgggc
     2821 aagatctacc gctttctcgg cctgaccgtc ggcgtcaacc tgccgcacat ggagcccgag
     2881 aagaagcgcg ccgcgtacgc cgccgacatt acctacggca ccaacaacga gttcggcttc
     2941 gactacctgc gcgacaacat ggcgctgcat gtcgaggagc gcttccagcg cggcctgcac
     3001 tacgcgatcg tggacgaggt ggactcgatc ctgatcgacg aggcgcgcac gccactcatc
     3061 atctcgggcc aggcggagga ccgaaccgag atctacctgc gcatgaacga ggtcgcgccg
     3121 ctgctcaccc ggcagaagga ggagaagaag cccgaccagc ccgagccgcc aggcgacttt
     3181 tacgtcgacg agaaaaacca ccagatcctg ctgtccgagg cgggccacca gaaggccgag
     3241 gagatcctca cccgcaaggg cctgctgccc gagggcgcga gcctctacga gcctgcctac
     3301 atcaacctgg tgcaccagct ctacgccgcg ctgcgcgcgc actatctctt ccaccgcgac
     3361 cagcattacg tggtgcagaa cgacgaggtg atcatcgtcg acgagtttac cggccggctg
     3421 atggtcgggc ggcgctggtc cgacggcctg caccaggcgg tcgaggcgaa ggagaaggtc
     3481 tcgatccaga acgagaacca gacgctcgcc tcgatcacct tccagaacta cttccgcatg
     3541 tacggcaagc tcgccggcat gaccggcacc gccgacaccg aggcctacga gttccagcag
     3601 atctacggcc tggagacggt ggtggtgccg acgcacatga agatgatccg
//
LOCUS       DASTPD010000013         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_617021, whole genome shotgun sequence.
ACCESSION   DASTPD010000013 DASTPD010000000
VERSION     DASTPD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617021"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VFR57_00400"
     CDS             <1..1273
                     /locus_tag="VFR57_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257870.1"
                     /translation="ENVRLFNEIKDSLEQQKASAEVLAAISSSIADTQPVFDKILRSC
                     ERLFAGKLVGINLVDPDGLIRIGAYQGPGREELEKLQPFAPGEGSGSGAAVLHRRLIQ
                     YPDAQKGEETPPATRRGCTAIGVKSVVFAPMIWEERAIGVIFVGRDFAGEFSEKEIAL
                     LKTFADQAVIAIQNARLFREIAEKSRQLEEASRHKSQFLASMSHELRTPLNAILGFNE
                     MILGQIYGEVPPDMKEPLEDIQKSGKHLLRLINNVLDLAKIEAGKMELALADYSVHDT
                     VESVRSTLRPLAEAKGLELVVSVPEDIPLAYGDGGRLTQCLMNLAGNSLKFTKQGKVE
                     IAVEAENGHLRWRVADTGIGIPPDKIPTLFSEFKQTDATIASEYGGTGLGLSITRKFV
                     EMHGGRIWVESEPGKGSVFTVEIPLRVTAPA"
     gene            1270..1644
                     /locus_tag="VFR57_00405"
     CDS             1270..1644
                     /locus_tag="VFR57_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0257871.1"
                     /translation="MSAKTILYIEDNEMNRKIVRDLLKRTKYTLIEAYDGEAGIAKAL
                     EARPDLILMDIQLPKISGLDATKRLRAEPVTAMTPIIAITSFALSGDDARAKEAGATA
                     YLAKPYSPFDLLTLIRKILPET"
     gene            1641..>3564
                     /locus_tag="VFR57_00410"
     CDS             1641..>3564
                     /locus_tag="VFR57_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0257872.1"
                     /translation="MSQRTRKRATKVGKRKAGKPATLQRRLAEALAREAASAEILASI
                     RRSRESPQAVLDAIVRNLQRLFGTAFAALFLARDGKIEIVAAAGKARFVRGMRAGGPT
                     RLEDEELLAVRAMRRAQVLRLCPIIGNPEAPPKTERLARRTGFDSIIAAPMLRDGVAI
                     GAVGTARTEAIPFDDAQVALIRSFADQAVIAIENARLFNDTKEALEQQTATAEILRVM
                     SSSPSDVQPVFDAVARKTSELCSGNYAIVVRFDGELIHLAAQHNARPGADQATARRYP
                     RPPGRETPSSRAILEQAVVHVPYADKDPDLPQDFVRRIGAGSFLSVPLLRQGRAIGAI
                     GASRAESGPFPDKQIELVKVFADQAVIAIENARLFNETKEALEQQTATAEVLNAISGS
                     VSDTAPVFEKILDSCQHLFATEELGIVVLKDDGLAHIAAWRGESMVPVMQTFPRPLAQ
                     TMTSRALTERRVIQVDDAAKLDPIPESLRAVVERVGHHSIAYAPMLAGDEAIGAIVVM
                     SVPPRPLNDKQLTQLKTFADQAVIAIKNARQFHETREGLERQTATAEILKVIASSPSD
                     TQPVFDAIAKSAQQLFAANSAAVARRVDDMLHLAAHTAMSEAGAESLRKLFPAQISGQ
                     GAFGKAVLTGKPAWIAD"
BASE COUNT          691 a         1153 c         1166 g          554 t
ORIGIN      
        1 cgagaacgtg cgcctgttca acgagatcaa ggactcgctc gaacagcaga aggcctccgc
       61 ggaggtgctc gcggcgatca gcagctccat cgccgatacg cagccagtct tcgacaagat
      121 cctgcgcagc tgcgagcggc tcttcgccgg caagctggtc ggcatcaacc tcgtcgaccc
      181 ggacgggctg atccgcatcg gcgcctacca ggggccggga cgcgaggagc tggagaagct
      241 gcagcctttc gcgccaggcg agggatcggg aagcggcgcg gccgtccttc accgccggct
      301 gatccagtat ccggacgcgc agaagggcga ggaaaccccg cccgccaccc ggcgcggctg
      361 cacggcgatc ggcgtgaagt cggtggtgtt cgcgcccatg atctgggaag aacgcgcgat
      421 cggagtgatc ttcgtcggcc gcgacttcgc cggcgagttc tcggagaagg agatcgcgct
      481 gctcaaaacg ttcgcagacc aggcggtaat cgccatccag aacgcgcgtc tgtttcgcga
      541 aatcgcggag aagagccgcc agctcgagga ggcgagccgg cacaaatcgc agttcctcgc
      601 cagcatgagc cacgagctgc gcacgccgct caacgccatt ctcgggttta acgagatgat
      661 cctcggccag atctacggcg aggtgccgcc cgacatgaaa gaaccgcttg aggacatcca
      721 gaagagcggc aagcacctgc tgcggctcat caataacgtg ctcgacctcg cgaagatcga
      781 ggcggggaag atggagctcg cgctcgccga ctattcagtg cacgacacgg tggagagcgt
      841 gcgttccacc ttgcgccccc tcgccgaggc gaaggggctt gagctcgtcg tgagcgtgcc
      901 cgaggacatt ccgctcgcct acggtgacgg cgggcggctc acgcagtgcc tgatgaacct
      961 ggccggcaat tccctcaagt tcaccaagca aggaaaagtg gagatcgcgg tggaggcgga
     1021 aaacggtcac ctgcgatggc gcgtcgccga caccggcatc ggcatcccgc cagacaagat
     1081 cccgacgctg ttctccgagt tcaaacagac cgacgccacg atcgccagcg agtacggcgg
     1141 cacgggcctc gggctttcca tcaccaggaa gttcgtcgag atgcacggcg ggcgtatctg
     1201 ggtggagagc gagcccggca agggctcggt attcacggtt gaaattcccc tgagggtgac
     1261 ggcgccggca tgagtgcgaa gaccatcctc tacatcgagg acaacgagat gaaccgcaag
     1321 atcgtgcgcg atctgctgaa gcgcacgaag tacacgctca tcgaggccta cgatggcgag
     1381 gccgggatcg cgaaagcgct cgaggctcgt cccgacctga tcctgatgga catccagctg
     1441 ccgaagatct ccggcctcga cgcaacgaag cggctgcgtg cggagccggt aaccgccatg
     1501 acgcccatta tcgccatcac ttcgtttgct ctctcgggcg acgacgcaag agcgaaggag
     1561 gcgggcgcca ccgcctacct cgccaagccc tacagcccgt tcgacctcct gactctcatt
     1621 cgcaagatcc ttccggagac atgagccagc gcacccgcaa gcgcgccaca aaggtcggca
     1681 agcgcaaggc gggcaagccg gccacgttgc agcgcaggct cgccgaggcg ctcgcgcgcg
     1741 aggcggcgag cgccgagatt ctcgcgtcca tccgccgctc cagggagagc ccgcaggccg
     1801 tcctggatgc gatcgtgcgc aacttgcagc ggcttttcgg cacggcgttc gcggccctgt
     1861 tcctcgcccg cgatggcaag atcgagatcg tcgcggcggc cggcaaagcg cgcttcgtcc
     1921 gcggcatgcg cgcaggcggt ccgacgcggc tcgaggacga agagctgctc gcggtgcgcg
     1981 ctatgcggcg cgcgcaagtg ctgcgcctgt gcccgatcat tggcaacccg gaggcgccgc
     2041 ccaagacgga gcgcttggcg aggaggaccg gcttcgactc gatcattgcg gcgccgatgc
     2101 tgcgcgacgg cgtcgcgatc ggcgccgtcg gcacggcgag gaccgaggcc attccctttg
     2161 acgacgcgca ggtcgccctg atccgctcgt tcgccgatca ggcggtcatc gcgatcgaga
     2221 acgcacgcct ctttaacgac accaaggagg cgctcgagca gcagacggcc acggccgaaa
     2281 ttctgcgagt catgtcgagc tcgcccagcg atgttcaacc ggtgtttgat gctgtggcgc
     2341 gcaagacaag cgagctttgc agcggcaatt acgcgatcgt cgttcggttc gacggcgagc
     2401 tgatccatct ggccgcgcag cacaacgcac gccccggcgc ggatcaggct accgctcggc
     2461 ggtacccgcg gcctcccggg cgcgaaacac cttcttcacg cgcgatcctt gaacaagccg
     2521 tggtccacgt tccctatgcg gacaaagacc cggatctgcc gcaggatttc gttcgccgca
     2581 taggagccgg gagctttctg tccgtgccgc tgctgcgcca gggcagggcg atcggcgcga
     2641 tcggtgcctc gcgcgccgaa tccgggccat tccccgacaa gcaaatcgaa cttgtgaagg
     2701 tattcgcgga ccaggcggta atcgcgatcg agaacgcacg cttgttcaac gagaccaagg
     2761 aggcgctcga gcagcagaca gcgaccgccg aggtgctcaa tgcgatcagc ggctcggttt
     2821 cggatacggc gccggtcttc gagaagattc tcgattcttg ccagcatctt ttcgccaccg
     2881 aagagctcgg cattgtagtg ctcaaggacg acggtctggc ccacatcgcc gcctggcgcg
     2941 gcgaatcgat ggtaccggtg atgcaaacct ttccgcggcc gttggcgcag acgatgacct
     3001 cgcgagcact cacggagcgc cgtgtgatcc aggtcgacga cgccgcgaag ctagacccga
     3061 tccctgagag cctgcgcgct gtggtcgaaa gggtggggca tcattcgatc gcctacgctc
     3121 ctatgctggc gggcgatgaa gccatcggcg caatcgtcgt catgagcgtg ccgccgcggc
     3181 cgctgaacga caagcagtta acgcagctga agaccttcgc cgatcaggcg gtgatcgcga
     3241 tcaagaatgc acgtcaattc cacgagacgc gggaggggct ggagcggcag acggcgaccg
     3301 ctgaaatatt gaaagtcatc gcgagctcgc cgtccgacac gcagccggtg ttcgacgcca
     3361 tagcgaaaag cgcgcagcag ctcttcgccg cgaactcggc ggcggtcgcg cggcgtgtcg
     3421 acgacatgct gcatctggcc gcgcacaccg cgatgagcga ggccggagcc gaatcgctca
     3481 ggaagctttt cccggcgcaa atatccgggc aaggtgcttt cggcaaggca gtgcttaccg
     3541 gcaagccagc ctggattgcc gacg
//
LOCUS       DASTPD010000014        18435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1637941, whole genome shotgun sequence.
ACCESSION   DASTPD010000014 DASTPD010000000
VERSION     DASTPD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637941"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2958
                     /locus_tag="VFR57_00415"
     CDS             190..2958
                     /locus_tag="VFR57_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0257873.1"
                     /translation="MATGEILKVIAGSPSDVQPVFETICKSAAQLCNAKDAVLWYVDG
                     DTMRVGGHFGDLRVEEVPVASSAPGRAVLDREVVHVRDLQAETVRFPRGSEVARRLGL
                     RTTVNVPLLHEGKAIGVISARRAEIQPFSDKQIALLKTFADQAVIAIENVRLFNETRE
                     ALERQTATSEILRVISQSPTDTQPVFKTIVASAARLCDADFSAVARFEGGLLHLVAVN
                     NMSAAETAAYHAVFPRAPGRHFAMGRAFVDGRPVHIENIETDPDYDPWTREVLSRAAA
                     YRTFLGVPILQNGLPIGAIGCGRHEMKPFTAPQIELVKTFADQAVIAMENVRLFNETK
                     EALEQQKASAEVLAVISNSIADTKPVFDKILESCTQLFGGHDSGIALVGDDGLVHLAA
                     HHGSRAEGLARAFPVPLSRESGTGTAIIERCVVHYPDTSGPDVPPYVRRVSEIIDFKS
                     VIFAPMFWEERGIGAIYVGRRFAAAFTEKQIALLKTFADQAVIAIENGRLFAQLQERT
                     AALTKSVGQLTALGEVGQAISSTLDLETVLKTIVSRAVQLARLDAGAIYEYDEPSEQF
                     HLRAAENISEEALAVYRSAPIRKGDGAVGGTAVGREPIQIPETQDESYKSQFRDLIIR
                     TGRRALLAVPLIHEEQIVGALAVQRNTPGPFAPEVVDLLKTFATQSALAIQNAKLFRE
                     IQEKSAQLEVANKHKSDFLANMSHELRTPLNAIIGFSEALMDQMFGDLNEKQLEYQKD
                     IHESGKHLLSLINDILDLSKIEAGRMELELSRFHLPTAVSNAVTLIRERAMRHGIALG
                     VDIDKRLGDLQADERKVKQILLNLLSNAVKFTPDGGRVDVSAKLDTDKIEIAVRDTGV
                     GIAPEDQAVLFEEFKQLGKDSSRKAEGTGLGLALTKRLVELHGGQIRVDSAVGHGSTF
                     RVILPSPT"
     gene            3311..4252
                     /locus_tag="VFR57_00420"
     CDS             3311..4252
                     /locus_tag="VFR57_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HEU0257874.1"
                     /translation="MANLAGLRSPSRALNCGAAILAGWLVFATAHARIIIEISENYVA
                     IEHEASRMISREKPYTASLPRIANNDSYSVLIGRVSAPLYVEVRDEANVPGKQAPVRF
                     AGSSLGGTQEVLPGLPRNAILTVSPEQEGASRLRLRVLRQGTRSHESLVATIRFLRES
                     TDRIEAVYRVPTITLRVRPCGVVNAFSSPDITLCTELMDTMFREGWHDAIRVVLYHEL
                     AHSLLRLWELPGYNNEDLADEFAVLHASDSPRAIEQISRWFESRDPLQKAATQVQMAG
                     PHAPSALRAKRARAGLTNRAQLRVKWDAFLAPYRRTR"
     gene            complement(4532..5101)
                     /locus_tag="VFR57_00425"
     CDS             complement(4532..5101)
                     /locus_tag="VFR57_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257875.1"
                     /translation="MVAGDKTLLAQQLGVPLPAVSDWLSGESPVPAEVFLRAVDIVLA
                     ANRKQNEENRALLERINDSDEKERKRATAELERDAAEARREQHNQDRQIAEADRVVAE
                     LKRVANEADRQTAEKLRAIAETFRKEYEALRKAAEETRAFAEQARDAAEAARAAAERS
                     AEVSRFAEDSRESAEESRQFGRRRDDRPQ"
     gene            complement(5281..5356)
                     /locus_tag="VFR57_00430"
     tRNA            complement(5281..5356)
                     /locus_tag="VFR57_00430"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5321..5323),aa:Lys,seq:ttt)
     gene            complement(5398..6386)
                     /locus_tag="VFR57_00435"
                     /pseudo
     CDS             complement(5398..6386)
                     /locus_tag="VFR57_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
     gene            complement(6383..7204)
                     /gene="ybgF"
                     /locus_tag="VFR57_00440"
     CDS             complement(6383..7204)
                     /gene="ybgF"
                     /locus_tag="VFR57_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU0257876.1"
                     /translation="MRLLLFLLASSLSAGALAQGIFDDNEARRRIDRLRQEVEAHRQA
                     TEARIAKAEQSAQEAAERAGDRGALIELSGQLEGLRSEMARMRGQLEVLNHQAETAEG
                     RQKQLYLDIDTRLRKLEQAREQQAAVPERPPAPTTPQPTAEPEPTPAEAKAYQAALDQ
                     FKLGNYTLAVAAMQGFLVTYPNSTLAPNAQYWIGMAHSGQRDYKSAIAAQRKLLSSYP
                     DSPKAPDAMLSIASAQETMGDRAAARKTLEELIAKYPGSSSATSAKQRLAASAKR"
     gene            complement(7204..7770)
                     /gene="pal"
                     /locus_tag="VFR57_00445"
     CDS             complement(7204..7770)
                     /gene="pal"
                     /locus_tag="VFR57_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU0257877.1"
                     /translation="MRLIVVLSALALLAGCQTTPEDQQPSAAGVEERKPGAKPGVQAK
                     PVERPQVGSVDATKPGAAGKSPAGALKDPSSPLSRRQIFFDYDKFEVKDEYRPVIEAH
                     AKYLRDNANAKMLVQGNADERGSREYNVALGQRRSDNVKKMLVLLGAREAQVEAVSLG
                     EEKPMCADHNEQCWAKNRRGDMLYGGEY"
     gene            complement(7785..9077)
                     /gene="tolB"
                     /locus_tag="VFR57_00450"
     CDS             complement(7785..9077)
                     /gene="tolB"
                     /locus_tag="VFR57_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEU0257878.1"
                     /translation="MALRDNNMGRFLLALLLSATALDAAAQLQIEITGAGVQRTPIAI
                     VSFPGEQNVGTSLTAIVRADLERSGLFRGLEIPPLAPHPNENSPINYGEWRSRLADAL
                     VVGSVAQNPDGRFEVRFRVYDVVKQAPMGAIAYLFTKDNARAAAHRIADFVYEKITGE
                     KGVFSTQIAYVVKRAEAYELQIADADGERAQYLLRSNEPIISPVWSPEGRRIAYVSFE
                     SKKPVVYVHHLSENKRQVVASFRGSNSAPAWSPDGKTIAVVLSVEGGSQIYLISADGS
                     GPRRRISNSASIDTEPRYSTDGKWIYFTSDRGGSPQIYRMASTGGEPQRVTFRGSYNV
                     SPRPSPDGRLLAYVTRDGGRFQVALLDLSNQQVQVITDSDRDESPSFAPNGRMILLAT
                     VKGGRGVLSAVSSDGRIRQHLPYSGGDVREPSWGPFIE"
     gene            complement(9079..9849)
                     /locus_tag="VFR57_00455"
     CDS             complement(9079..9849)
                     /locus_tag="VFR57_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0257879.1"
                     /translation="MSTLAWDVWRHQERGMGPAFALSALMHILLVAILVLGVRWQVSA
                     PDIVTVDLVEALPPPPPPAPPAPVVQPPKPVPAPEPKPLPRVEKPQIAIPDKPKPKPK
                     PEPKPAPPKEDPEFQKRLQEQMAEEQKNLEIQRQERELRELIAKQQARQQADAARAAS
                     AARAKALNEYILSIQIKVKSGWILPQDIQGNPQAIFDVVQLPTGEVLSTKLVRSSGNP
                     AYDAAVERAILKASPLPLPANRELFARELRLTFRPLDK"
     gene            complement(9846..10274)
                     /locus_tag="VFR57_00460"
     CDS             complement(9846..10274)
                     /locus_tag="VFR57_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEU0257880.1"
                     /translation="MALHKKRRPVHEINVVPYVDVMLVLLVIFMVTAPLVTPAVIDLP
                     TVDKASAPRVVPLEIFVKSDQGLVVRQRDARGGVVNEQSLSRAQLAGFIKSVRAKNQD
                     VSVLIGGDKNARYEAVLQVMDELRKQGVQKVGLQVKTAQQ"
     gene            complement(10274..10957)
                     /gene="tolQ"
                     /locus_tag="VFR57_00465"
     CDS             complement(10274..10957)
                     /gene="tolQ"
                     /locus_tag="VFR57_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEU0257881.1"
                     /translation="MTLTQDLDIWTLILHASIVVKVVMLLLVAVSFMSWMFIFQKWFA
                     IRRAHSQTDKFEREFWSGNDLNALYQGAVNHRHTVGSLERIFEAGFREFAKLRSGQRG
                     GTDASNMVDGARRAMRAAFQREMDFLERHLAFLASTGSVSPYVGLFGTVWGIMHAFRS
                     LANVQQATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRYSHDIERLANRFESFMEEF
                     SNILHRQVR"
     gene            complement(10954..11322)
                     /gene="ybgC"
                     /locus_tag="VFR57_00470"
     CDS             complement(10954..11322)
                     /gene="ybgC"
                     /locus_tag="VFR57_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEU0257882.1"
                     /translation="MYYQDTDAGGVVFHAQYLSFMERARSELLNEAGIELAAFVERHG
                     TLFMVHRIDVKYHRPASLNDVLSVSAEVAKMGRASLVFHQRVERGTELLVEADVTLAL
                     VERRRMKPVRVPDELEKALR"
     gene            complement(11337..12353)
                     /gene="ruvB"
                     /locus_tag="VFR57_00475"
     CDS             complement(11337..12353)
                     /gene="ruvB"
                     /locus_tag="VFR57_00475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0257883.1"
                     /translation="MIAARPASPQEEQFERSLRPSTLAEYVGQEKVRAQLEIFIAAAR
                     GRSEALDHVLLFGPPGLGKTTLAHIVAKELGVNLRQTSGPVLERPGDLAALLTNLEPK
                     DVLFIDEIHRLPPVVEEILYPALEDFQIDIMIGEGPAARSIKLDLPPFTLVGATTRAG
                     MLTNPLRDRFGIVSRLEFYGEADLTRIVQRSARLLKVALEDDGAREIARRSRGTPRVA
                     NRLLRRVRDYAQVKADGRVSKALAEAALKMLDVDALGLDMMDRKLLLAVIEKFGGGPV
                     GLDNLAHAVGEEADTIEDVLEPYLIQQGYLQRTPRGRIATLLAYRHFGIVAPAAMKDL
                     LSDT"
     gene            complement(12394..12942)
                     /locus_tag="VFR57_00480"
     CDS             complement(12394..12942)
                     /locus_tag="VFR57_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU0257884.1"
                     /translation="MRISFAALLAIPVAIVASPSAASEERGFYIGGALGQATFKDWCV
                     PSPLVLSCDDGTTAWKIFGGYRFNPYFGAEATYVDWGQVSGTVSGVGTVTADQTSFGV
                     AAVGRLELTPQFSVFGKAGLLITEQETPASSTRKRDETELHYGFGARYLFAPNWAARA
                     EWERTDQLKVELLSIGLEFRFQ"
     gene            complement(13142..13636)
                     /locus_tag="VFR57_00485"
     CDS             complement(13142..13636)
                     /locus_tag="VFR57_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU0257885.1"
                     /translation="MSRKWLVSVLGAAAVAMSVGAQAQTGGAPLVPNIYAGIELGQAD
                     VGNEEDVGFKIFGGYQFTRNLAAEIGYGMLFDKNDVEITALELVAVGIFPLANQFSLI
                     GKLGLANVEADTRFGSEDKTELTYGFGVQFDMSRNLGLRALWQRYDTDEEVDWITIGA
                     LWRF"
     gene            complement(13806..14372)
                     /gene="ruvA"
                     /locus_tag="VFR57_00490"
     CDS             complement(13806..14372)
                     /gene="ruvA"
                     /locus_tag="VFR57_00490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0257886.1"
                     /translation="MIGRLSGRLAAKQPPQVLVDVGGIAYEVDVPMSTFYNLPATGEA
                     VSLYTHLVVREDAHTLYGFATLDERAAFRQLIRISGIGARTALSVLSGLSVGDLANAV
                     TMQDAAALTRIPGIGKKTAERLLLELKGKLAETAAPAGTQAASDVMNALVALGYSDKE
                     AAAAVKGVSPSMPVAEAIRAALKTLAKS"
     gene            complement(14423..14881)
                     /gene="ruvC"
                     /locus_tag="VFR57_00495"
                     /pseudo
     CDS             complement(14423..14881)
                     /gene="ruvC"
                     /locus_tag="VFR57_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
     gene            complement(14878..15600)
                     /locus_tag="VFR57_00500"
     CDS             complement(14878..15600)
                     /locus_tag="VFR57_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257887.1"
                     /translation="MAGHSRWANVKHRKERADAKRGKIFTRLIREITVAARLGGGDPA
                     GNPRLRLALEKAREQNLPGDNIQRAIQRGSGELDGTAYEEIRYEGYGPGGAAVMLDCL
                     TDNRTRTVAEVRHAFTKNGGNLGSDGSVAYLFKHCGQFVFAPGTSEDKVMEAALDAGA
                     EDVFRNDDDSVELVCAPPDFAAVKAALAKAGLKPELAEITMKPTMENPLAGDEAARMR
                     RLLDALEGLDDVQDVYTTAVVD"
     gene            15608..17077
                     /locus_tag="VFR57_00505"
     CDS             15608..17077
                     /locus_tag="VFR57_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEU0257888.1"
                     /translation="MILRMLLAKNEKSALSLLPGLANRHGLITGATGTGKTITMQALA
                     QRLSDLGVPVFMADVKGDLSGMAKSGGANAKVLERFQSLKLKPEFAGCPVEFWDVYGK
                     NGLPVRATVSDMGPLLLGRMLGLNETQEGVLGLVFKVADDNGLLLLDLKDLRAMLQHV
                     GDNAAQYKTQYGNVTGASIGAIQRGVLALENQGAAQFFGEPMLEIADLMQLRGGKGVV
                     NILTAEQLMNSPKLYSSFLLWLLAELFEQLPEVGDPDKPKLVFFFDEAHLLFEEAPKA
                     LTEKVEQVVRLIRSKGVGVYFVTQNPLDVPESVLGQLGNRVQHALRAFTPRDQKAVKT
                     AAETLRPNPKLKAEKVITELGVGEALVSLLDEKGRPAVVERAFIVPPASQVGPIGQDE
                     RQTIINSSKLLSKYEEAIDRESAYEKLKAQVQEKQPESASNPISDLLLGKTGPRGGRQ
                     TQGVLEAVTKSAARAVGSELGRQIMRGVLGSILGGGRRR"
     gene            17074..17958
                     /locus_tag="VFR57_00510"
     CDS             17074..17958
                     /locus_tag="VFR57_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0257889.1"
                     /translation="MSARAWGIVFAFVGVLAFSFRPILIKLSYAAHPVSPVTLLFLRM
                     TLSLPFFLVIGWWLRNQEPRLSKRDWAAIAGLGFLGYYAASFLDFVGLQWVGAGVGRL
                     ILYLFPTLVLLLSFLFLHKKPTWREIAALVLSYAGIALVLSNQLAIESRLFALGAALV
                     FASALCYAAYLVAGSQVVKRVGSMRFTAYSTVVATVPAVLQFAVMESPGALDLPPVVW
                     GYAIVLATVVTVLPLFLQAEALHRIGANHFALIGAVGPVSVAVTSALGLDEPFTGIQA
                     LGGALVIFGVLLVSLKRS"
     gene            17962..>18435
                     /locus_tag="VFR57_00515"
     CDS             17962..>18435
                     /locus_tag="VFR57_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257890.1"
                     /translation="MDLGIRGRTALVCAASKGLGKGCAMALAREGVNLVITARGREAL
                     EATAAELRSTFKVKVDAIAGDITTEEGRKAALAAAPSPDILVNNAGGPPPGDFRAWDR
                     DTWVKALEANMITPILLIRAVVDGMVARKFGRVVNITSGAVKAPIDVLGLSNGARS"
BASE COUNT         3155 a         5958 c         6000 g         3322 t
ORIGIN      
        1 cgctagcacg ggcgtagcat agtggggcat ctgcttgttg ccgatccatg ggaggaggca
       61 accgtgccac aacgacgtcg caaaacctcg tcgaagcagc gttctcggcc tgcgcggcag
      121 actattgacc gcctgcgcct tgccctgaaa cgagagcgcg aggaacgcaa ggaggcgctc
      181 gaacggcaga tggcaaccgg ggagattctg aaggtcatcg cgggctcgcc ctcggacgtg
      241 cagccggtgt tcgagaccat ctgcaagagc gcagcgcagc tatgcaatgc gaaggacgcc
      301 gtcctttggt acgtcgatgg cgacaccatg cgtgtcggtg ggcacttcgg ggatctgcgc
      361 gtcgaggaag tccccgtcgc gagttcggcg cccggccgtg ccgtcctcga ccgcgaggtc
      421 gttcacgtgc gcgatctgca ggcggaaacg gtccgcttcc ccaggggctc cgaggttgcc
      481 cgccgtctgg gactgcgcac gacggtcaac gtcccgctat tgcacgaagg gaaggcgatc
      541 ggcgtgatct cggctcggcg ggcggagatc cagcccttca gcgacaagca gatcgcgcta
      601 ctcaagacct tcgccgatca ggcggtgatc gcgatcgaga acgtgcgcct gttcaacgag
      661 acgagggagg cgctcgagcg ccagactgcg acgagcgaga ttctgcgagt gatcagccag
      721 tcgccgactg acacccagcc ggtgttcaag accatcgtgg ccagcgcggc ccgactatgc
      781 gatgcggact tcagcgccgt ggcgcgcttc gaaggcggcc tactccatct cgtggcggtc
      841 aacaacatgt ctgcggccga gactgcggcg tatcacgccg tttttccgcg agcgccggga
      901 cgacacttcg caatgggtcg ggctttcgtc gatggtcgcc cggtacacat cgagaatatc
      961 gagacggatc ccgactacga cccgtggacg cgagaggtgc tgtcgcgcgc ggccgcctat
     1021 cggacgttcc tgggagttcc cattttgcag aatggcctgc cgatcggcgc gatcggatgt
     1081 ggccgacacg agatgaaacc atttacggcc ccccagatcg agctcgtaaa gaccttcgcc
     1141 gatcaggcgg tgatcgcgat ggagaacgtg cgcctcttca acgagacgaa agaggcgctc
     1201 gagcagcaga aggcctcggc cgaggtgctc gcggtgatct cgaactcaat cgccgatacc
     1261 aagccggtgt tcgacaagat cctcgagagc tgcacccagc tgttcggcgg ccacgattcg
     1321 gggatcgccc tcgtcggcga cgacgggctg gtccacctcg ccgcgcacca cggctcgcgc
     1381 gcggaagggc tggcgcgggc gttcccggtt ccgctgagcc gggaatcggg caccgggacg
     1441 gccatcatcg agcggtgcgt agtgcattat ccggacacct cagggcccga cgtgccgcct
     1501 tacgtgcgcc gcgtatccga aatcatcgac ttcaagtccg tgatctttgc gccaatgttt
     1561 tgggaagaga ggggaattgg cgccatctac gtcggccgcc ggttcgcggc cgccttcacc
     1621 gagaaacaga tcgcgcttct caagaccttc gccgatcagg cagtgatcgc gatcgagaac
     1681 gggcgcctgt tcgcacagct gcaggagcgt accgcggcgc ttacgaaatc ggtggggcag
     1741 ctcaccgcgc tgggcgaagt cgggcaggcg atcagctcga cgctcgactt agagacggtg
     1801 ctgaagacga tagtgtcgcg cgctgtgcag ctcgcacggc tcgacgccgg agcgatctac
     1861 gaatacgacg agcccagcga gcagttccac ctgcgcgccg ctgagaacat cagtgaggag
     1921 gctctagcgg tttaccgcag cgcgcccatc cggaagggcg acggcgccgt gggcggcacg
     1981 gcggtcgggc gcgagccgat tcagatcccc gagacacagg acgagagcta caagagccag
     2041 ttccgcgatc tcatcatccg caccggtcgc agagcactgc tcgcggtacc gctcatccac
     2101 gaagagcaga tcgtcggcgc gctcgccgtg cagcggaaca cgcccgggcc gttcgcgccg
     2161 gaggtcgtgg acctcctcaa gacgttcgct acgcagtcgg cactcgccat ccagaacgcc
     2221 aaactcttcc gcgagattca ggagaagagc gcgcaactag aagtggcgaa caagcacaag
     2281 tccgatttcc tcgccaacat gtcgcatgag cttaggacgc cactcaacgc catcatcggc
     2341 ttttccgagg cactcatgga tcagatgttc ggcgacttga acgaaaagca gctcgagtac
     2401 cagaaggaca tccacgaatc cggcaagcat cttctttcgc tcatcaacga catcctcgac
     2461 ctctcgaaga tcgaggcggg caggatggag ctcgagttat cgcgcttcca tctccccacg
     2521 gcggtgtcca atgcagtgac cctcatccgc gaacgcgcga tgcgccatgg cattgcgttg
     2581 ggcgtggata tcgacaagcg gcttggggac cttcaggccg acgagcgcaa ggtaaagcag
     2641 atcctgctca acctgctatc caacgccgtg aagttcactc cggacggtgg gcgggtggac
     2701 gtgagcgcga agctcgatac cgacaagatc gagatcgcgg tgagggacac aggcgtcgga
     2761 atcgcgccgg aggatcaggc cgtacttttc gaggagttca agcagctcgg caaggatagt
     2821 tcccgcaagg ctgaaggcac gggacttgga ctagccctga ccaagcgcct cgtggagctg
     2881 cacggcgggc agatccgagt cgacagtgcg gtgggccatg gctccacatt tcgcgtgatc
     2941 cttccgtcgc ccacctaatg catgctgagg accgtgttca gcggggtggt gctggcgccc
     3001 ttactcatcg cattgatctc ctgcggcggc gggagcgacg acgagccgga gcggcacgaa
     3061 gtctcgggat tgatttccat ctcccagccc agtcaagggt cctcaacatc cagcgcaacg
     3121 gcgctcgtga gaggccctcg cagcaatacc gtccacaccg tcacttggag taattcagcg
     3181 ggtgggaatg gctcagccac cctcgggtca tgcgttttct ttcctttgcc gcttccactt
     3241 ccttgctggg agacgtcgat cccgttgtca gtcggcgcga acgtcatcac ggtgatcggg
     3301 gacggtagtg atggcgaatt tagccgggct acgatcacca tcacgcgcac ttaactgcgg
     3361 ggctgccatc ctcgctgggt ggctcgtctt cgcgaccgcg cacgcgcgca tcatcatcga
     3421 gatctccgag aactacgtgg cgatcgagca cgaggcgagc cgcatgattt ctcgcgaaaa
     3481 gccgtacacg gccagcctgc cgcgcatcgc caacaacgac agctactcgg tgctcatcgg
     3541 ccgcgtctcg gcgccgctct acgtagaggt gcgcgacgag gccaacgttc ccgggaaaca
     3601 ggcgcccgtg cgcttcgctg ggtcgtcact gggcggaacg caggaagtgc tgcccggtct
     3661 gccgcgtaat gccatcctga cggtgagccc cgaacaggag ggcgcgtccc gcttgcgcct
     3721 gcgcgtgctg cgccagggca cgcgctctca cgaatcgctt gtggcaacca tacgtttcct
     3781 gcgcgaatcc accgatcgca tcgaagcggt ttatcgcgtc ccgaccatta cgctgcgagt
     3841 gcggccctgc ggggtggtga atgcgttctc cagtcccgac atcaccctgt gcaccgagct
     3901 gatggatacg atgttccgcg agggttggca tgacgcgatc cgggttgtcc tctaccacga
     3961 gctcgcgcac tctctgctgc gtctgtggga gctgccggga tacaacaacg aggatctcgc
     4021 cgacgagttc gcggtgctgc acgcgagcga ttctcccagg gcgatcgagc agatcagccg
     4081 ctggttcgaa tcgcgcgatc cccttcagaa ggcggccacg caggtgcaga tggccggccc
     4141 gcatgcgcca tcagctctgc gcgccaagag ggcgcgcgcg gggttgacca atcgggcgca
     4201 gctcagggtg aagtgggatg cgttcttagc tccttatcgg cggacccgtt agccaggccg
     4261 ctcgcaccct tcggcggcta cagaactatc gttaccgtgc cgatgctgcg ccaatcagag
     4321 cccatcggcg ccatcgctat ctaccgcacg gaagtggcgc cgttcgccga ccgacacata
     4381 gcgttgctgc aaacattcgc caatcaggcc gtgatcgccg tcgataacgc tcacccgctg
     4441 gatctgctag cggtgcgcgt cctctcgcgg gttttctcca accaatcaac taacgcgagc
     4501 ctatgcgctc gcctcgggcc agcgagtctc gttattgcgg cctatcgtcg cgccgccttc
     4561 cgaattggcg gctttcctcg gcagactccc gcgaatcttc cgcgaagcga gaaacttcag
     4621 cggaccgctc cgctgccgct cgagcggctt cggcagcgtc ccgagcctgt tctgcgaaag
     4681 cacgcgtttc ctctgcggcc tttcgcaatg cctcatattc ttttcggaac gtttcggcga
     4741 tcgcacggag cttctcggcg gtttgcctgt ccgcctcgtt tgccacgcgc ttgagttctg
     4801 ccaccacgcg atccgcttcg gcgatttgcc ggtcttggtt gtgttgttca cgacgagctt
     4861 cggcggcatc acgctccagc tctgctgtag cgcgtttgcg ttccttttcg tcggagtcat
     4921 tgatccgttc gaggagggcg cggttctctt cattttgctt tcgattcgcg gccaacacga
     4981 tgtccaccgc acgcaggaat acttcagcag gcacggggct ttcacccgac aaccagtccg
     5041 aaacggccgg tagaggaacg ccgagctgtt gggcgaggag cgtcttgtca ccagcgacca
     5101 tgcaggcccg catcaacacc gtttggtatg tctcccgata ccccacgctg gaaccctcgc
     5161 taggtcctca ttacacacct cttcgagcaa cgcagcaata acactctgca aaggtacgac
     5221 gtagcaagtc gcagcgcagc acgccacgtt ggctgtgctc cggtaagcaa atgatcttgt
     5281 tggtaggtcg tggcggattc gaaccgccga ccaacggatt aaaagtccgc tgctctacca
     5341 gctgagctaa cgacccacgc tacgacgcgg atttgctgcg gaaagggcgg gattttatca
     5401 gcccgcgcgc cagtattggt acttcatggt cgcggcagag ccggcgacga tggcgagaaa
     5461 ggtaatgatt gagccgagcg cgagggtgga aaagccgttg attgcctggc cgatggtgca
     5521 gccgagcgcg gtgataccgc cgaatcccat gagcacgccg cccacgatgt gattggcggt
     5581 gtcctcggcg gtgcggaacc cttcccagcg gaacgtccgc gtggcgagcg cataggctgc
     5641 agagcccacg atcacgcccg cggccgacgc gatgccgtaa gtgatgatct tggacttgtc
     5701 ggtccagagc atcagatact cgagcgtgta ggcgaacggc gcgacgaacg agaacgattc
     5761 catgcggccg gtattggtgg cgacgaatgc ctcctgcagt gtgttcggat cctcttcgac
     5821 gtgaccgaga aagccgctga cgtaccagcc gccgacgacc acgaggccgg tgacgacgcc
     5881 gcccagcgtg tagtcgaagc tcgagcggaa ctgccggctc gcgtaaacga aggcgatgat
     5941 ggcgccgccg aagagcgcgg cgagcagcgc ccggctcatg ttaagaagcg cgggcaggtc
     6001 ctgcccctga gggagcgtga tgctcgcttt ttcgagcacg ccgacgcgga atgcgccgaa
     6061 cagcccgcgc agcgtcatat aggcggcgat gcccaggaag atgtacacca cgagcgactt
     6121 gagattcccc gcgccgacgc ggatcaaggt cttcgagccg cagcccgagc cgagtgtcat
     6181 gccgacgccg aagaggaatc cgccgacgag gtgcgaaagc caggtgaagt tcgggggagc
     6241 cacatgcgaa ggcggctcgt gtcgcccatg ttgacccagt cggagacggc gcccatggtg
     6301 cagaagtgcg tcttgttgcc gaccgcgcca aaaatggcgg cgaggaggaa ggctcccccg
     6361 gcaacgtaca gatgcgcttc gttcaacgct tggcggaagc ggcgaggcgc tgcttcgcgc
     6421 tcgtcgcaga gctgctcccc gggtacttgg cgatcagttc ttccagcgtc ttgcgcgccg
     6481 cggcgcggtc gcccatggtt tcctgggcgc tcgcgatgct gagcatcgcg tcgggtgcct
     6541 tcgggctatc cggataggag gacagcagct tgcgctgcgc ggcgatcgcg cttttgtaat
     6601 cgcgctggcc ggaatgcgcc atgccgatcc agtactgcgc gttcggcgcg agtgtgctgt
     6661 tgggatacgt aacaaggaaa ccctgcatgg ccgcgacagc aagcgtgtag ttgccgagct
     6721 tgaactggtc gagcgccgcc tgataggcct tcgcttcggc gggtgtgggc tcgggctccg
     6781 ccgtaggctg tggcgtcgtc ggcgcgggcg gcctttcggg cacggcggcc tgctgctcgc
     6841 gcgcctgctc cagcttgcgc agtcgcgtat cgatgtcgag atagagctgc ttttgccgtc
     6901 cctcggcagt ctcggcctga tggttcagca cctcgagctg gccgcgcatg cgcgccatct
     6961 cgctgcgcag gccttcgagc tgcccggaga gctcgatcag cgcgccgcgg tcgccggcgc
     7021 gctcggccgc ctcctgcgcc gactgctccg ccttggcgat ccgcgcctcg gtggcctggc
     7081 ggtgcgcttc gacttcctgc cgcagtcggt cgatgcgccg gcgcgcctcg ttgtcgtcga
     7141 agatgccctg cgccagcgcg cccgcgctga ggctagaggc gaggaggaag agaagaaggc
     7201 gcatcaatac tcgccgccgt agagcatgtc gccgcgccgg ttcttggccc agcattgttc
     7261 attgtgatcg gcgcacatcg gcttctcctc gccgaggctc accgcctcga cctgcgcctc
     7321 gcgcgcgccg agcaggacca gcatcttctt gacgttgtcg gagcgccgct ggccgagcgc
     7381 gacgttgtac tcgcggctgc cgcgctcgtc ggcgttgccc tgcacgagca tcttggcgtt
     7441 ggcgttgtcg cgcagatatt tggcatgcgc ctcgatcacc ggacggtact cgtccttcac
     7501 ttcaaatttg tcgtaatcga agaagatctg gcggcgcgaa agcgggctgg acggatcctt
     7561 cagggcgccg gccgggctct tgccggcggc gccgggcttg gtcgcgtcca cactgccgac
     7621 ctgcgggcgc tcgaccggct tcgcctgcac gccgggcttc gcgcccggct tgcgctcttc
     7681 gacaccggcc gcggagggct gctgatcttc gggagtggtt tggcaccccg caagcaacgc
     7741 gagggcagac aacacgacga tgaggcgcat gcagttcgct cctttcattc gatgaacggg
     7801 ccccaggacg gctcccgcac gtcgccgccc gagtaaggca ggtgctggcg gatgcgtccg
     7861 tcggaggaca ccgccgacag cacgccgcgg ccgcccttca ccgtggcaag caaaatcatc
     7921 ctgccgttcg gggcgaagct cggcgactcg tcgcgatcgc tgtcggtaat gacctgcact
     7981 tgttggttag acaaatcgag cagcgccacc tggaaccggc cgccgtcgcg ggtcacatag
     8041 gccagaagac ggccgtcggg gctggggcgc ggcgaaacat tatagctgcc ccggaaggtg
     8101 acgcgctgcg gctccccgcc ggtggaggcc atgcggtaga tctgcgggct gccgccgcgg
     8161 tccgatgtga aataaatcca cttcccgtcg gtggaatagc gcggctcggt gtcgatcgag
     8221 gcggagttgc tgatgcgccg ccgcgggccg ctgccgtcgg cgctgatgag gtagatctgg
     8281 gacccgccct cgaccgagag gaccaccgcg atggtcttgc cgtccggcga ccatgccggt
     8341 gccgaattcg aaccgcggaa gctcgcgacg acctgacgct tgttctccga caggtgatgc
     8401 acgtaaacca ccggcttctt cgactcgaac gacacgtagg cgatgcgccg gccctcgggc
     8461 gaccagaccg gcgagatgat cggctcgttg gagcgcagca ggtactgggc gcgctcgccg
     8521 tccgcgtcgg cgatctgcag ctcgtaggcc tcggcgcgct tcaccacgta ggcgatttgg
     8581 gtcgaaaaga cacccttctc cccggtgatt ttctcgtaga cgaagtcggc gatgcggtgc
     8641 gccgcggcgc gtgcgttgtc cttggtgaag aggtacgcga tggcgcccat tggcgcctgc
     8701 ttcaccacgt cgtagacgcg aaagcgcacc tcgaagcggc catcggggtt ctgcgccacc
     8761 gacccgacga cgagcgcatc ggcgaggcgc gagcgccatt cgccatagtt gatgggcgag
     8821 ttctcgttcg gatgcggcgc gagcggcggg atttcgagcc cgcggaaaag gccgctgcgc
     8881 tccagatccg cgcgcacgat tgccgtgagg ctcgtgccga cattctgctc gcccgggaac
     8941 gagacgatgg cgatcggcgt tcgctgcacg cccgccccgg tgatttcgat ctgaagttgc
     9001 gccgcggcat ccagcgccgt ggcggacaga agaagagcta gaaggaagcg ccccatgttg
     9061 ttgtcccgaa gggccatgct atttgtccag cggccggaaa gtaagtctca gttcccgggc
     9121 gaatagttcc ctattggcgg gcagcggcaa cggagaggct ttcaggattg ctcgttccac
     9181 cgccgcgtcg taggccggat tgccgctcga gcgcacgagt ttggtcgaaa gcacctcgcc
     9241 ggtcggaagc tgtacgacgt cgaatatcgc ctggggattg ccctggatat cctgcggcag
     9301 gatccagccc gacttcacct tgatctggat acttaagatg tattcgttca atgccttcgc
     9361 gcgtgcggcg gaagcggcgc gcgctgcgtc ggcttgctgg cgcgcctgct gcttggcgat
     9421 cagctcgcgc agctcgcgct cctggcgctg aatctccagg tttttctgct cctcggccat
     9481 ctgctcctgc aggcgctttt ggaactcggg atcctccttg ggcggtgcag gcttgggctc
     9541 gggcttgggc ttcggtttgg gcttgtccgg aatggcgatc tggggctttt cgacccgggg
     9601 cagcggcttc ggttcgggcg ccggcaccgg tttgggcggc tgcaccaccg gcgcgggcgg
     9661 cgcgggcggc ggtggcggcg gaagcgcctc gaccagatcg acggtcacga tatccggcgc
     9721 ggagacctgc cagcgcacgc cgaggaccag gatcgcgacg agcaggatgt gcatcagggc
     9781 cgacagcgcg aacgccgggc ccatgccgcg ctcctggtgc cgccacacgt cccaggcgag
     9841 cgtgctcact gctgcgcggt cttcacctgc agaccaacct tctgcacgcc ctgcttgcgc
     9901 agctcgtcca tcacctgcag caccgcttca tagcgcgcgt tcttgtcgcc gccgatcagc
     9961 acggagacat cctggttttt ggcgcgcacg cttttgatga agccggcgag ctgcgcgcgg
    10021 ctgagagact gctcgttcac cacgccgccg cgcgcgtcgc gctggcgcac cacgagaccc
    10081 tgatcgctct tcacgaagat ctcgagcggc acgacgcgcg gcgcgctcgc cttgtcgacg
    10141 gtcggcagat cgatgacagc gggcgtcacg agcggcgcgg tcaccatgaa gatgacaagc
    10201 agcacgagca tcacgtcgac gtagggcacg acgttgatct cgtgcaccgg ccggcgcttc
    10261 ttgtggagcg ccatcagcgc acctggcgat gcaggatgtt cgagaactct tccatgaagc
    10321 tctcgaagcg gttggcgagg cgctcgatgt cgtgcgagta gcggttatac gcgaccaccg
    10381 cgggaatcgc ggcgaagagg ccgatcgcgg tggcgacgag cgcctcggcg atgcccggcg
    10441 ccacctgcgc gagcgtcgcc tgctggacgt tggcgaggct gcggaacgca tgcatgatgc
    10501 cccacaccgt gccgaacagg ccaacgtacg ggctcacgga gccggtcgag gcaaggaatg
    10561 cgagatggcg ctcgaggaaa tccatctcgc gctggaacgc ggcgcgcatg gcgcggcgcg
    10621 cgccgtcgac catgttgctc gcgtcggtgc cgccacgctg cccgcttcgt agcttcgcga
    10681 actcgcggaa tcccgcctca aagatgcgct ccaggctgcc gacggtatgg cggtggttca
    10741 ccgccccctg gtagagcgcg ttcaagtcgt tgccgctcca gaactcgcgc tcgaacttgt
    10801 cggtctgcga gtgggcgcga cggatggcga accacttctg gaaaatgaac atccaggaca
    10861 tgaaggacac ggcgacgagc agcagcatca ccaccttgac cacgatgctg gcgtgcagga
    10921 tgagcgtcca gatgtcgagg tcttgggtga gcgtcatcgc aaagcctttt cgagttcgtc
    10981 gggcacgcgc accggcttca tgcgccggcg ctcgaccagc gcgagcgtca cgtcggcttc
    11041 gaccagaagc tcggtgccgc gttccacacg ctggtggaac acgaggctcg cgcggcccat
    11101 tttagccact tcggcgctca ccgacagaac gtcgttgagg cttgcgggcc ggtgatattt
    11161 cacgtcgatg cgatggacca tgaagagcgt gccgtggcgc tcgacgaacg cggcgagctc
    11221 gatgccggcc tcgttcaaca gttcgctgcg cgcgcgctcc atgaacgaca ggtactgcgc
    11281 atggaacacg acacctccgg catcggtgtc ctggtaatac acccggaccg ggatcttcag
    11341 gtatcggaca ggagatcctt catcgccgcc ggcgcgacga tgccgaagtg gcgataggcg
    11401 agcagcgtgg cgatgcgccc gcgcggcgtg cgctgcagat agccctgctg gatcaggtag
    11461 ggctccagca cgtcttcaat ggtgtcggct tcctctccca ctgcgtgcgc caggttgtcg
    11521 agtcccaccg gcccgccgcc gaatttctcg atcaccgcga ggaggagctt gcggtccatc
    11581 atgtcgaggc cgagcgcgtc gacgtcgagc atcttgagtg ccgcctcggc gagcgccttc
    11641 gacactctgc cgtcggcctt tacttgcgcg tagtcgcgca cccggcgcag cagccggttc
    11701 gcgacgcgcg gcgtgccgcg cgagcggcgc gcgatctcgc gcgcgccgtc gtcctcgagc
    11761 gcgactttca gaaggcgcgc cgagcgctgc acgatccgcg tgaggtcggc ctcgccgtag
    11821 aactccaggc gcgacacgat gccgaagcgg tcgcgcaacg ggttggtgag catgccggcg
    11881 cgggtggtcg cgccgaccag cgtgaacggc ggcaggtcga gcttgatgga gcgcgccgcc
    11941 gggccctcgc cgatcatgat gtcgatctgg aagtcctcca gtgccggata gaggatctcc
    12001 tcgaccaccg gagggaggcg atggatctcg tcgatgaaga gcacgtcctt cggctcgaga
    12061 ttcgtgagga gagccgcgag atcgccgggg cgctcgagca ccggcccgga cgtctggcgc
    12121 aggttcacgc cgagctcctt ggcgacgatg tgcgcgagcg tggtcttgcc cagccccggc
    12181 ggcccgaaga gcagcacatg gtcgagcgct tcgcttcgcc cgcgcgccgc ggcgatgaag
    12241 atctcgagct gcgcgcggac cttttcctgc cccacgtact cggcgagcgt ggacggacga
    12301 agcgagcgtt cgaactgctc ttcctgcggg gacgcgggcc gcgccgcaat catccggtcg
    12361 ggttcgatgc tcaccgacct gattttgcca gacctactgg aagcggaact ccaagccgat
    12421 ggagagcagc tccaccttga gctgatcggt gcgctcccac tcggcgcgcg cggcccagtt
    12481 gggggcaaaa aggtagcgcg ctccgaagcc gtaatggagc tcggtttcgt cgcgcttgcg
    12541 cgtgctcgag gcgggtgtct cctgctcggt tatcagcaat ccggcctttc cgaacaccga
    12601 gaactgcggc gtgagctcga gccggcccac tgcggcgacc ccgaagctcg tctggtcagc
    12661 cgtcaccgtg ccgacaccgg atacggtgcc gctgacctgg ccccagtcta cgtaggttgc
    12721 ttccgcacca aagtacgggt tgaagcggta gccgccaaag atcttccacg ccgtggtgcc
    12781 gtcgtcacag ctgaggacga gcggcgacgg aacgcaccaa tctttgaacg ttgcctgacc
    12841 aagcgcgccg ccgatataga agccccgttc ctcggacgcc gccgagggtg aggcgacgat
    12901 ggcaaccgga atggccagga gcgcagcaaa cgagatccgc atccgccttt tcgaacagct
    12961 cgaacctgaa ttgttcccga tcggccgtgc ggcgcgggat atgtcgtgaa tccgtaacgc
    13021 ccgcctatcc cagatgacac gccgtaaccc tcttccattg ccaatattgc atagcccaaa
    13081 aacaacaacg ccccggaagg attccggggc gttttctagt atgcgactgg tgttcttctt
    13141 tttagaacct ccacagcgcg ccgatggtga tccagtccac ttcctcgtcg gtgtcatagc
    13201 gctgccacag ggcgcgcagg ccgaggttac ggctcatgtc gaactgcacg ccgaaaccgt
    13261 aggtgagctc cgtcttgtct tcgcttccga accgggtgtc ggcttccacg ttggccaagc
    13321 cgagcttgcc gatcagcgaa aactgattgg cgagtgggaa gatgccgacg gcgacgagtt
    13381 cgagcgcggt gatttcgacg tcgttcttgt cgaagagcat gccatagcca atctcggccg
    13441 cgaggttccg agtgaactgg tagccgccga agatcttgaa cccgacgtct tcctcgttgc
    13501 cgacatccgc ctggccgagc tcgatgccag cgtagatgtt cggcacgagc ggcgctccgc
    13561 cagtctgcgc ctgcgccccg acggacattg caaccgcggc cgcgccaagt accgaaacca
    13621 gccacttcct gctcataaga ctcctttcgt ctcgtgttgg tcaaacgcga aatgcgcgag
    13681 cgaagcttag gataggcata tttcgtggac gaccgccgca gtggaaaacg accgcttgga
    13741 agcgaaacgc ccgcctccat gttgcagaaa tgcaacgggc gtcaagcttc agtgtcacta
    13801 agtcactacg acttcgcgag ggttttgagc gcggctctga tcgcctcggc gaccggcatg
    13861 ctgggcgaga cgcccttcac cgcggcggcc gcttctttgt cgctgtagcc gagcgcgaca
    13921 agtgcattca tcacgtcgga cgcggcttgc gtaccggcag gcgcagccgt ttcggcgagc
    13981 ttgcccttga gttcaaggag caaccgctcg gctgtcttct tgccgatgcc cgggatgcga
    14041 gtgagcgctg ctgcgtcctg catcgttacc gcgttcgcga gatcgccgac agaaagacca
    14101 gagagcacgg atagcgctgt gcgcgcgccg ataccggaga tgcgaataag ctggcggaac
    14161 gccgcgcgct catcgagtgt ggcgaacccg tagagtgtgt gcgcgtcctc gcgcaccacg
    14221 agatgcgtgt aaaggctcac cgcttcgccg gtggccggca ggttgtagaa cgtactcatc
    14281 ggcacgtcga cttcataggc tatgccaccc acgtcgacga gcacctgcgg cggctgcttg
    14341 gctgcgagcc gtcccgaaag ccgcccgatc aaacgaagcg acccttgcgc atgcgaaagc
    14401 cggtgtgcac gccgaggcgg ctgccatggg catgacagat cgcgcaggcg agcgcgtcgg
    14461 cggcgtcggg ggcagggtcc gccggcaacg cgagcaggcg cttcaccata tgctgcacct
    14521 gctcctttgc cgcgtgtcca ttgccgacta cggactgctt cacttgcagg gcggtgtatt
    14581 cagcgaccgg caggccggcc agcaccgcgg cgcagatcgc ggtaccgcgc gcctgcccaa
    14641 gtgcgagcgt ggattgcgga ttcgaattca cgaaaacctt ttccaccgcc acctcgccgg
    14701 gcatgtgcgc ggcgatcact tccttcaagc cttcgagtat ggttttgagg cgcccggcca
    14761 ggtcgccggc ccccgatttg acgcaaccgc tcgcgatgta atgaaggcgg gagtttcgtt
    14821 cgctctcgat cactccgaat cccgtgacct gcagacccgg atcgatcccg aggatcctca
    14881 atcgacgacc gccgtggtgt agacgtcctg aacgtcgtcg aggccttcga gggcgtcgag
    14941 cagccgccgc atgcgcgccg cttcgtcgcc cgcgagcggg ttctccatgg tgggcttcat
    15001 ggtgatctcg gcaagctcgg gtttgagccc ggctttggcg agcgcggcct tgacggccgc
    15061 gaagtcgggc ggcgcgcaca ccagctcgac cgaatcgtcg tcgttcctga agacgtcctc
    15121 cgcgcccgcg tcgagcgccg cctccatcac cttgtcctcg ctcgtgccgg gagcgaagac
    15181 gaactgcccg caatgcttga agaggtaggc gaccgagccg tcggagccga ggttgccgcc
    15241 gttcttcgtg aaggcgtggc gcacctcggc caccgtgcgc gtgcggttgt ccgtgaggca
    15301 gtcgagcatc accgccgcgc cgccgggacc gtagccctcg tagcggattt cctcgtacgc
    15361 cgtgccgtcg agctcgcccg agccgcgctg gatcgcgcgc tgaatgttgt ctcccgggag
    15421 attctgctcg cgtgcctttt ccagagcgag gcgcagccgc gggttgccgg ccggatcgcc
    15481 accgcccagc cgcgccgcca ccgtgatctc ccggatgagg cgcgtgaaga tcttgccgcg
    15541 cttcgcgtcg gcgcgctcct ttcggtgctt gacgttggcc catcggctgt ggccggccat
    15601 ggtgcttata attttacgca tgctcttggc caagaatgaa aagtcggcac tctcccttct
    15661 gcccgggctt gccaatcgcc atggcctcat caccggcgcc accggcaccg gtaagaccat
    15721 caccatgcag gcgctcgcgc agcggctctc cgatctcgga gtgccagtct tcatggcgga
    15781 tgtgaagggc gatctctccg gcatggccaa atccggcggt gcgaatgcca aggtcctcga
    15841 gcgcttccag tcattgaagc tgaagcccga gttcgccggt tgcccggtcg agttctggga
    15901 tgtctacggc aagaacgggc tgccggtgcg cgcgacggta tcggatatgg gtccgctcct
    15961 tctcgggcgc atgctcggcc tgaacgaaac gcaggaaggc gtgctcgggc tcgtattcaa
    16021 ggtcgcggac gacaacgggc tcctgcttct cgatctcaag gatttgcgcg cgatgctgca
    16081 gcacgtcggc gacaacgccg cgcaatacaa gacccagtac ggcaacgtca ccggtgcgtc
    16141 gatcggcgcc atccagcgcg gcgtcctcgc cctcgagaac cagggcgcgg cgcaattctt
    16201 cggcgagccg atgctcgaga tcgccgacct catgcagctg cgcggcggca agggcgtggt
    16261 gaatatactg acggccgagc agctgatgaa ctcgccgaag ctctattcga gcttcctttt
    16321 gtggctgctg gccgagctct tcgagcagct gcccgaagtg ggcgatccgg ataaacccaa
    16381 gctcgtcttc ttcttcgacg aggcgcacct ccttttcgag gaagcgccga aagcgctcac
    16441 cgagaaagtg gagcaggtgg tgcgcctcat ccgctcgaaa ggcgtgggcg tgtacttcgt
    16501 gacgcagaac ccgctcgacg ttccggagag cgtgctcgga cagctcggca accgcgtgca
    16561 gcatgcattg cgcgccttca cgccgcgcga ccagaaggcg gtgaaaaccg ccgccgaaac
    16621 actgcggccc aaccccaagc tcaaggccga gaaggtcatc accgagctcg gcgtcgggga
    16681 ggcgctggtc agcctgctgg acgaaaaagg ccgcccagca gtggtcgagc gcgcgttcat
    16741 cgtcccgcct gcgagccagg tcggccccat cggccaggac gagcggcaga cgatcatcaa
    16801 ttcgtccaag ctgctctcca agtatgaaga ggccatcgac cgcgagtcgg cgtacgagaa
    16861 gctgaaggct caggtacagg agaagcagcc cgagtcggcg tcgaacccga tttccgatct
    16921 tctgctcggc aagacggggc cgcgcggcgg ccggcagacg cagggtgtgc tcgaggccgt
    16981 gacgaagagc gccgcgcgcg ccgtcggctc ggagctcggg cggcagatca tgcgcggcgt
    17041 tctgggatcg atcctcggcg gcggccgccg ccgttgagcg cacgcgcctg gggcatcgtc
    17101 ttcgcgttcg tcggcgtcct cgctttctcg tttcggccga tcctgatcaa gctgtcgtac
    17161 gccgcgcacc cggtgagccc ggtcacgctg cttttcctgc gcatgacgct gtcgttgccc
    17221 tttttcctcg tcatcggctg gtggctgcgc aatcaggaac cgcgcctatc caagcgcgac
    17281 tgggccgcga tcgccggcct tggcttcctc ggctactacg ccgccagctt tctcgacttc
    17341 gtcggcctgc agtgggtcgg cgccggcgtc ggccgcctca tcctctacct cttcccaacg
    17401 ctggtcctgc tcctctcgtt cctcttcctc cacaagaagc cgacgtggcg cgagatcgcg
    17461 gcgctggtgc tgagctatgc gggaattgcg ctcgtccttt ccaaccagct tgccatcgag
    17521 agccggctct tcgcgctcgg cgccgcgcta gtcttcgcct ctgcgctctg ctatgcggca
    17581 tacctcgtcg ccggaagcca ggtggtgaag cgcgtcggtt ccatgcgatt caccgcgtac
    17641 tcgacggtgg tggcgaccgt gcccgcggtg ctgcagttcg ccgtcatgga atcgcccggt
    17701 gcgctcgact tgccgccggt ggtctggggc tacgccatcg tgctcgccac cgtcgtcacc
    17761 gtgctgccgc tcttcctgca ggccgaagcg ttgcatcgca tcggggccaa ccactttgcg
    17821 ctcatcggcg cggtcggacc ggtcagtgtc gcggtcacga gcgcgctcgg cctcgatgag
    17881 ccgttcacag ggatccaggc gctcggcgga gcgctcgtca tcttcggggt tctcctcgtt
    17941 tcgctcaaaa ggagctagtc catggacttg ggaattcgcg ggcgcaccgc gctcgtttgc
    18001 gcagcatcga aaggtctggg gaaaggctgc gccatggcgc tggcgcgcga aggggtgaac
    18061 ctcgtcatca ccgcgcgcgg gcgcgaagcg ctagaagcaa ctgcggccga gctccggtcg
    18121 acattcaaag taaaggtcga tgcgattgcg ggagacatca cgacggagga aggacgcaag
    18181 gcggcgctcg ccgccgcgcc gtcgcccgac atcctggtga acaatgcggg gggcccgccg
    18241 cccggcgact tccgcgcatg ggaccgcgac acctgggtga aggcgctcga ggccaacatg
    18301 atcacgccca tcctcctcat ccgcgccgtg gtcgacggca tggtggcgcg caagttcgga
    18361 cgcgtcgtca acatcacctc gggcgccgtg aaggcgccga tcgacgtgct gggcctgtcg
    18421 aacggcgcgc gcagc
//
LOCUS       DASTPD010000015         4295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1025946, whole genome shotgun sequence.
ACCESSION   DASTPD010000015 DASTPD010000000
VERSION     DASTPD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025946"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..793
                     /locus_tag="VFR57_00520"
     CDS             173..793
                     /locus_tag="VFR57_00520"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0257891.1"
                     /translation="MDFKLYCFAQSGNAYKAALMLNLCGAKWEPRFVDFFNGETRTPE
                     YRANINEMGEVPVLEHNGKRLSQSGAILHYLAEHLGKFQGEDRMEVLRWILWDNHKLT
                     SYIATLRYMVTLAKVGEPQVHEFLRGRVKGSLVILDLHLKKSPFVAGKEPSIADLSLC
                     GYLYWPDEFGVSWAEFPAVGAWLERIRKLPGWVHPYELMPGHPLKR"
     gene            797..2317
                     /locus_tag="VFR57_00525"
     CDS             797..2317
                     /locus_tag="VFR57_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0257892.1"
                     /translation="MLQAVELTIRLHPDDDVVIARMEIPTGTLVTRENVRVAVTIPAG
                     HKLAIRDIAQGKPVRRYNQIIGFATRDIRAGEHVHVHNLAMGDFERDYAFCSLQKPTE
                     SISPSATFQGIVRADGCVATRNYIGILTSVNCSATVARMVAKHFENDIDDYPNVDGVV
                     ALTHKTGCGMAGEGEGMELLRRTLTGYAQHPNFYAVLNIGLGCEANQINFWLDGSQLK
                     LSQKLQAFTIQEKGGTTKAVKDGIARIKELLPDANRIERRSVPASHLILGLECGGSDG
                     YSGISANPALGAAADLLARHGGAAILSETPEIYGAEHLLTRRAVSKEVGEKLLARIRW
                     WEDYTARNRMEMNNNPSPGNKAGGLTTILEKSLGAAAKGGTTNLVEVYEFAEKVTAKG
                     LVFMDTPGYDPVSVTGHVAGGANVICFTTGRGSVYGCKPAPSLKLATNSRLYQHMEED
                     MDVNCGTIVDGVETVEQVGERIFRLILETASGKKTKSELLGFGEDEFAPWVVGATM"
     gene            complement(2340..2819)
                     /locus_tag="VFR57_00530"
     CDS             complement(2340..2819)
                     /locus_tag="VFR57_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0257893.1"
                     /translation="MKAPPFELPGIDGKRHSPQSVRGDKGLLVMFICNHCPYVKRVID
                     GVVRDCKALAALAIGSIAVMSNDPADYPEDSFENMKRVAREKGFPFPYVIDEMQDVAR
                     AYDTVCTPEFFGFNAALELQYHGRFDEMFKAMKQIAETGKGPKEQIPAVGCSIKWKK"
     gene            2834..3556
                     /locus_tag="VFR57_00535"
     CDS             2834..3556
                     /locus_tag="VFR57_00535"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU0257894.1"
                     /translation="MAPRFYIDAPLRVGSVCTLSEDAAHHAIHVLRLRAGEEITVFNG
                     RGGEFAARIVSIQRLKIAVDLLHRREIERESPLRVTLVQSVSAGDKMDSTLRKAVELG
                     VAEVQPVLAARSVARPKGERAEGRRAHWQKVVIAACEQCGRNRIPEVHALIALSNYRP
                     SGTAMRILLSPLAQQPLSELSLRGSEFVVAAGPEAGFTSEEEAALVSSGFAPALLGPR
                     VLRTETAAVAALAALSALRGDS"
     gene            complement(3531..4142)
                     /locus_tag="VFR57_00540"
     CDS             complement(3531..4142)
                     /locus_tag="VFR57_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257895.1"
                     /translation="MLSASEDYPVKPIHTLLIAILLIPALPAHAAGEESPALGHALTL
                     IHLFVRIAAESETPQQSLKAVDDVLLGRNAAANEAAAGLMKEMLADVPPEHRATVAAI
                     GRDLAAVARKNLKVPDGSSLSTTPLQARRDLTSMGLAYHDSKQFLDAVKRNDALAAEL
                     FIAGRGVDLSARDFWGRDAVDFARANGNERLAELIAKNRPAER"
BASE COUNT          801 a         1372 c         1389 g          733 t
ORIGIN      
        1 ggcgagaacg agatccagag acgtagatcg agctggaaag cacccaaggc ccccctggat
       61 cgcggctatt ggaagctcta tttcgatcac gtgctccagg cgcatcaagg cgcggatctc
      121 gacatcctgg tgggcaagag cggcgcgttc gtgccgcgcg acaaccactg acgtggattt
      181 caagctctac tgcttcgcgc aatccggcaa cgcctacaaa gctgcgctca tgctcaacct
      241 gtgcggcgcg aaatgggagc cgcgcttcgt cgacttcttc aatggagaga cgcgcacgcc
      301 ggagtaccgc gcgaacatta acgagatggg ggaagtgccg gtgctcgagc acaacggaaa
      361 gcgcctctcc caatccggag cgatcctcca ttacctcgcc gagcacttgg gcaagttcca
      421 gggcgaagac cggatggagg tcctgcgctg gatcctctgg gacaatcaca agctgacgag
      481 ctacatcgcc acgctgcgct atatggtcac gctcgccaag gtcggcgagc cccaggtgca
      541 cgaattcctg cgcgggcgcg tcaaggggtc gctcgtcatc ctcgaccttc acctcaagaa
      601 gagtcctttc gttgctggaa aggagccgtc gatcgccgat ctttccttgt gcggctacct
      661 gtactggccc gacgagttcg gcgtaagctg ggcggagttt ccggccgtcg gcgcgtggct
      721 ggagcggatc cgcaagcttc ccggctgggt gcatccgtac gagctgatgc ccggccatcc
      781 actgaagagg tagcgcatgt tgcaagccgt cgagctcacc atccgcctgc atcccgacga
      841 cgacgtagtc atcgcccgca tggagatccc gaccggcacg ctcgtgacgc gcgagaacgt
      901 gcgggtcgcg gtgaccatcc cggccgggca caagctcgcg atacgcgaca tcgcgcaggg
      961 caagccggtc cgccgctaca accagatcat cggcttcgcc acgcgcgaca tcagggcggg
     1021 cgagcacgtg cacgtccaca acctcgcgat gggcgacttc gagcgggact atgctttttg
     1081 ctctttacaa aagcccactg agtccatttc gccgagcgcc accttccagg gcatcgtgag
     1141 agctgacggg tgcgtcgcga cgcgcaacta catcggtatc ctcacgagcg tgaactgctc
     1201 cgcgacggtc gcgcggatgg tcgcgaagca tttcgagaac gatattgacg actacccgaa
     1261 tgtggacggc gtggtggcgc tcacgcacaa gaccggctgc gggatggcgg gcgaaggcga
     1321 aggaatggag ctcctgcggc gcacgctgac cggctatgcg cagcatccga acttctacgc
     1381 cgtgctcaat atcggcctcg gctgcgaggc gaaccagatc aatttctggc tcgacggctc
     1441 gcagctcaag ctatcgcaga agctgcaagc cttcaccatc caggaaaagg gcggcactac
     1501 gaaggccgtg aaggacggca ttgcgcgcat caaggagctg ctgccggacg ccaaccggat
     1561 cgagcgacgc agcgtgccgg cctcgcacct catccttggg ctggaatgcg gcggctcgga
     1621 cggctattcc ggcatcagcg ccaatccggc gctcggcgct gcggccgacc tcctggctcg
     1681 ccacggcggt gcggcgatcc tgtccgaaac ccccgaaatc tacggcgccg agcatttgct
     1741 tacacggcgc gcagtgtcca aggaagtcgg cgaaaaactg ctggcacgca ttcgctggtg
     1801 ggaggactac accgcacgca accgcatgga gatgaacaac aacccctcgc ccggcaacaa
     1861 ggcgggcggg ctgaccacga ttctggagaa gtcgctgggc gccgccgcca aaggcggaac
     1921 gacgaacctc gtcgaggtct acgaattcgc ggaaaaagtg accgccaagg gcctcgtctt
     1981 catggacacg ccgggctacg acccggtatc ggtcacgggg cacgtcgccg gcggcgcgaa
     2041 cgtcatctgc ttcaccaccg gccgcggctc ggtgtacggc tgcaagcccg cgccttcgct
     2101 caagctcgcc accaactcac gcctctacca gcacatggaa gaggacatgg acgtcaactg
     2161 cggcacgatc gtcgacggcg tggagaccgt cgagcaggta ggcgagcgca tcttccggct
     2221 gatcctcgag accgcctcgg gcaagaagac gaagtcggaa ctgctcggct ttggcgagga
     2281 cgagttcgcg ccctgggtcg tgggcgccac catgtagcag aatcggggtc agagcccgat
     2341 tattttttcc acttgatgga gcagcccacc gcagggatct gctccttcgg tcccttgccg
     2401 gtttcggcga tctgtttcat ggccttgaac atctcgtcga agcggccgtg gtactggagc
     2461 tcgagcgcag cgttgaagcc gaagaactcc ggcgtgcaca ccgtatcgta ggcgcgcgcg
     2521 acgtcctgca tctcgtcgat cacataggga aacggaaagc ccttctcccg cgccacgcgc
     2581 ttcatgttct cgaacgagtc ctcgggatag tcggccgggt cgttcgacat caccgcgatc
     2641 gacccgatgg cgagcgccgc gagcgccttg cagtcccgca cgacgccgtc gattacgcgc
     2701 ttcacgtacg ggcagtggtt acagatgaac atcaccagca aacccttgtc gccgcggacg
     2761 ctttgcggcg agtggcgctt gccgtcgatg ccgggaagct cgaacggagg tgccttcatg
     2821 caaaaatcgt aacatggcac cgcgctttta catcgatgcg ccgctgcgcg tcggcagcgt
     2881 gtgcacgctt tccgaggacg cggcgcacca cgcgatccac gtgctgcgtc tgcgtgcggg
     2941 cgaagagatc accgtcttca acggccgcgg gggcgagttc gcggcgcgca tcgtgtccat
     3001 tcagcggctc aagatcgcgg tcgacctcct ccaccgccgc gagatcgagc gcgaatcgcc
     3061 attgcgcgta acgctggtgc agagcgtgtc cgccggggac aagatggaca gcacgctgcg
     3121 taaggcagtc gagctcggcg tggccgaagt gcaacccgtg ctcgccgcgc gctcggtcgc
     3181 gcggccaaaa ggcgagcgcg ccgagggccg acgcgcgcat tggcagaagg tggtgatcgc
     3241 cgcctgcgag cagtgcgggc gcaaccgcat tccggaggtg catgcgctga ttgcgctctc
     3301 caactaccgc ccgagcggca cggcgatgag gattctgctt tcccctctcg ctcaacaacc
     3361 gctttccgag ttgtccctgc gaggaagcga gttcgtggtc gcagcaggtc ccgaagccgg
     3421 attcacctcg gaagaggaag cggccctcgt gtcgagcggc ttcgctccgg ccttgctggg
     3481 gccgcgggtg ctgcgcaccg agacggcggc ggtggccgcg ctcgctgcgc tcagcgctct
     3541 gcggggcgat tcttagcgat cagctcggca aggcgctcgt tgccgttggc gcgagcgaaa
     3601 tccaccgcgt cgcggcccca gaaatcgcgc gccgaaagat cgacgccgcg accggcaatg
     3661 aagagctcgg cggcaagggc gtcgttgcgt ttcaccgcgt cgaggaactg cttcgagtcg
     3721 tggtacgcga gtcccatcga ggtcagatcc ctgcgcgcct gtaatggggt tgttgaaagc
     3781 gaagaaccgt cgggtacttt gagatttttc cttgccacgg cagcaaggtc gcgcccgatc
     3841 gcagcgacgg tggcgcggtg ctcgggcggg acgtcggcaa gcatctcctt cattaggcca
     3901 gcggcagcct cgttcgccgc cgcattgcgc ccgagcagca cgtcgtcgac cgccttcagg
     3961 ctctgctgcg gtgtctcgga ctccgcagcg atgcgcacga acagatggat cagcgtgagc
     4021 gcgtggccga gtgccggcga ctcctcgccg gcggcgtgcg ctggcaaagc cgggatgagc
     4081 agaatagcga tgagcagcgt gtggatgggt ttcaccgggt agtcctccga agcgctgagc
     4141 atagcgcagc ctgcaggttt tcggcccccc ggtagaattt gaaaaaacta cacatggata
     4201 gctcggggaa cttcgtccac tggttccggc aggtcgcgcc gtacgtgcac gacttcggcg
     4261 gacgcacgtt cgtgatcgcc ttcggcggcg agatg
//
LOCUS       DASTPD010000016        12917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1230259, whole genome shotgun sequence.
ACCESSION   DASTPD010000016 DASTPD010000000
VERSION     DASTPD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230259"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..901)
                     /locus_tag="VFR57_00545"
     CDS             complement(14..901)
                     /locus_tag="VFR57_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0257896.1"
                     /translation="MAEQASEAAILFADVSGSTKLYETAGNAVAHAAVESCVNIMRQK
                     TQAAKGRVIKTIGDEVMSAFRSADDAASAAMEMQLAISELPPIGSTTLGIRIGFNYGQ
                     VVERDGDVFGDAVNVASRLAAVASKGQIITARDTVLMMSPRLKASTRAITTIPAKGKA
                     HEIQVYELMWQHSQDMTTLASHKAVFRPRNTTLRLKMHETEVVLSAVRPSLAMGRDQS
                     ADLVITERMASRAHGKIERRLDKFVLTDHSANGTFVTIEGDKEIILRREEFTLRGHGW
                     IAFGQSRAGATDVVEFFCE"
     gene            complement(953..2161)
                     /locus_tag="VFR57_00550"
     CDS             complement(953..2161)
                     /locus_tag="VFR57_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257897.1"
                     /translation="MSAAVLPQHPAPPRPWRELWLITIGHGLTHWYPATFYLLLPLIG
                     AELGLSYSQIGLIMTCKYIASAVANVPGGVLVDTVGRKGLLMGLSLFWIGFPYLLIGF
                     THSYVMLLACIALVGFGNSLWHPTAIPTLGRRYPERKGLVLSVHGMGGNVGDAVAPVV
                     IGAALTVFTWREVVMLNVVPGLVVAALLFVFLGSMRLSSKKTEEKTQSFGAYLAGLKN
                     LFLSRSLVLLSTGSAFRTMTQSALLTFLPVYLANDMGYSPFWVGACLFALQAAGFAAS
                     PVAGHLSDRMGRKHILMTSMAASALVLAAMALSGGSPLFVALVAVLGFFLYATRPVIQ
                     AWMLEATPKDMGGSSIGVLFGAQAVGGALGPLLGGMVADRFGLAAAFYFLAATIVVAN
                     LFVIGVRTRN"
     gene            complement(2158..2772)
                     /locus_tag="VFR57_00555"
     CDS             complement(2158..2772)
                     /locus_tag="VFR57_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0257898.1"
                     /translation="MAWDESRAWRKGRHNQKLYQALLDDAAAAPARNAKRKKIVRPED
                     MPWEMSRQGLLKHLINEGMNTRMETVDAYMLIIPPGSRSGKHRHLAEECLYVLEGRGY
                     DLHQDCDVEITDTYHWKPQAEVKRYEWEAGDVIYIPPNTIHQHFNASAERPVRLISAT
                     NRIYKACGLNDLEQLEDAPEYDPKVVLTAELVEKFLRVKEKTPA"
     gene            complement(2774..3331)
                     /locus_tag="VFR57_00560"
     CDS             complement(2774..3331)
                     /locus_tag="VFR57_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0257899.1"
                     /translation="MSTESHYHSKKDRIFVRAIQGQYSLKEELARLRAMPRVRKGREL
                     KFVDGPQTYSRHYVEPKDGVTQMFHLHLEEYAPGGKSQKHGHVNEAAFYILDGKGFEI
                     HDGIRYDWQAGDVAIVHNNCVHQHFNASPDKPARALVIKTKPMFMFMNMLFQQLVEPR
                     PKTPAPGAEGYEVRPAEHNFNHSED"
     gene            3357..4484
                     /locus_tag="VFR57_00565"
     CDS             3357..4484
                     /locus_tag="VFR57_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257900.1"
                     /translation="MTVYLVVLLSMLNSIGQRGSKVAISLYALELGAGAAAVGMLAAL
                     FATFPLLLAVQAGKISDRFGVRIPIICGALTMAVGLAVAYMVSDIHWLFVCPALIGLG
                     HIFFHVSIHNLVGSLGADAERTRNFATFALGSSLAAFVGPSLAGFAIDLAGFRPAFAV
                     LAAVAAVPALIVLLYRSLIPTRVRHDEEEQARSAFDLLAIPALRRTLIMSGVTLTGIE
                     LFSFYLPIYGRSIGLAPSVIGMILSSYAVAGFIVRSFMHRLAQRYTEVGVLTGSLFVA
                     ALAYFIVPGLADGWLLAAAAFALGLALGSAQPLTIILTYNHAPAGRAGEALGMRIMAN
                     KVTQIAVPLAFGGFGAALGAVPVFLATGAFLLVAGLISLKR"
     gene            4527..5810
                     /locus_tag="VFR57_00570"
     CDS             4527..5810
                     /locus_tag="VFR57_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0257901.1"
                     /translation="MRAEQNEFLTRTGPGTPMGELFRRYWLPALLAAELPEPDCPPAR
                     VRILSEKLIAFRDTQGRLGLIDEFCAHRGVSLWFGRNEEGGLRCSYHGWKYDVTGQCL
                     EIPSEPDNPKLCQRMKLKSYPLVERGGVLWTYMGPRQLQPPLPEHEWAMVPQSHRFVS
                     KRWQECNYLQAMEGGIDSSHVSFLHRETIKVDPMFKGAAQSHQYNLQDLAPHFEVVES
                     AGGLYIGARRNAGDGKHYWRITQWVMPSFTLIPPRGDHPIGGHCWVPMDDENCWAWST
                     NHHAARALTQEEREAMEAGKGIHVPVIPGTFRPVANKDNDYLMDRRAQKERRTFSGVE
                     GFAMQDASVQESMGPIQDRTKENLMPTDQGIIMARRRLLAAAQGLQEGATPPGTDPRH
                     QRVRSVSIILPQGVSFSEAAEDALVPEPGSAHATV"
     gene            5822..6880
                     /locus_tag="VFR57_00575"
     CDS             5822..6880
                     /locus_tag="VFR57_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0257902.1"
                     /translation="MLARTIAVALLLVSTAATAQPAPGPHRSLYMYQGADRDQRIIEG
                     AKKEKQVVVYTSLNTKDSVPISQAFEKKYGVKVELWRSSSEKVLQRALTEARAGRHAV
                     DAFELNGPEMEALYREGLLEEFWSPYFKNLPPAAFAKHKHYVADRFNFFTIAYNTKLV
                     KPEEVPNSYEDLLHPRWVGKIGIEASDDDWFASIVKHMGEQKGLAFFRKLAEMKPQVR
                     TGHTLLAELVAAGEIPLVATIYNHNAERLLVNGAPIKWKALDPTFGRPNAVAVTKRAP
                     RPYAAMLFVDFMLSPEGQELLKQRNRVPASTAVETNLNKFPFHMIDPVIVLDEQAKWE
                     KIWSELFLKGQAIKREAD"
     gene            6882..7436
                     /locus_tag="VFR57_00580"
     CDS             6882..7436
                     /locus_tag="VFR57_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0257903.1"
                     /translation="MGKESHYSSKKDRVFVRAIQGQYSLKDELKRLRSLPRVIRGKEM
                     KFQDGPQSFSKHFVEPVDGMTQTLHIHLEEYAPGGRTQKHGHVNEAAFYILDGKGYEI
                     HDGVRYDWQAGDVAIVHNNCVHQHFNADPDKPARALVLKSKPMFMFMNMLFQQSVIPR
                     PTEPATPAGAGFEPRHDETHYNHE"
     gene            7433..8548
                     /locus_tag="VFR57_00585"
     CDS             7433..8548
                     /locus_tag="VFR57_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0257904.1"
                     /translation="MKSAVVSQDFAAKFATERETPYTRWVREEGLDIIGSFYVQNLHT
                     VELKPWPRRGGKGVFLNHDASRTSNDCYVCEIPPGKSLAPQRQLFEEMIYVLDGRGST
                     AVWNNAGNRASFEWKAGAIFAIPLNCWHQHFNGSGKDPVRYVAVTNAPVIINAFGDLD
                     FVFNCDHDFKERFAGEPEYFANKGEQKGLLLDTNFVADAINLPLFAAKERGAGGGHTR
                     FSMAKGSMNSHISQFPVGTYKKAHAHGPGAHVIVLSGEGFSLMWPEGEAPKRYDWREG
                     TMIVPPNLWFHQHFNTGTTPARYLAFKAEGVAVRNAQGVPKAWISRRLGGDQIDYADE
                     RPEIRQWFAEALAKHGVKPNMDKAYAMERGELPEKAA"
     gene            complement(8545..8904)
                     /locus_tag="VFR57_00590"
     CDS             complement(8545..8904)
                     /locus_tag="VFR57_00590"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU0257905.1"
                     /translation="MLKAYSSEVVHTGAVGSAQVAKAVNNLIMWACLVADHEGLALAQ
                     RYGVDVQMLRRALGMSSAANSALEKWGTQTMAWAEDDMAIVAQMAAECGLALPQAGLT
                     REICRALKPRRFRLDKY"
     gene            complement(8878..9357)
                     /locus_tag="VFR57_00595"
                     /pseudo
     CDS             complement(8878..9357)
                     /locus_tag="VFR57_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            complement(9333..11045)
                     /locus_tag="VFR57_00600"
     CDS             complement(9333..11045)
                     /locus_tag="VFR57_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0257906.1"
                     /translation="MKRWASDVIVDMLHAYDFPYAALNPGASYRGLHDSMVNYGGNRP
                     AMMLCQHEETAVQIAHGYAKASGRPMVAILHNLVGLLHANMAIYYAYADRAPVFIIGA
                     TGPMDETKRRPRIDWIHSALVQGEAVRAYTKWDYQPTVIDGVPESFARAYAVMMTEPR
                     GPIYMCYDAWLQEQPLEHEVALPKSSAAKVPSPLAPDPAALERAAEMLAAAKRPAIIA
                     EYVGREPAGFHALVELAETLGAPVYDVNSRLNFPSRHPLNLSMVKHVFRDADLILCLD
                     TRDWERPTTELVSTTREVTSLVPADCKWIDIGFGDLEISSWAMDYQRLAHADLRILAD
                     TTLAIPTLTKLLASRPVKRDSWLRTVAELRQKAHAKWQAEAKEDWDASPITLPRLATE
                     VWNAIKSEDWVLAAGTLDDRARQIWDFDKPYRHAGKSLGTATQIGISLGVALAHRDAK
                     RLVVDLQPDGDLMFDAGALWVAAKHRIPLLVVMYNNRAYYNDWEHQIRMAKLRGTPAE
                     RAHIGMDMDDPAPDFAALAKSMGWYAEGPIGRPGDVAAALRRAIARVKAGQPALLDTI
                     TQKR"
     gene            complement(11042..12160)
                     /locus_tag="VFR57_00605"
     CDS             complement(11042..12160)
                     /locus_tag="VFR57_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257907.1"
                     /translation="MLSVSALCTEYAGEHGQTIRAAQDVSFDVPQGKLFTLLGPSGCG
                     KTTTLRSIAGLEKPRSGEIVVGGRVIYSSTQRVFVPPNRRGLGMVFQSYAIWPHMTVF
                     DNAAFPLQVSRKSMPKIQIHERVHRVLKVVALEEMADREATKLSGGQQQRLALARALV
                     MEPSLLLLDEPLSNLDAKLRERMRFELKRLQRELGITTVYVTHDQSEALALSHSIAVM
                     NAGRIEQLGSPREVYERPRNQFVADFVGTTNFIKGDVSAADAAGGFYRIRSEIGELKA
                     RGVDSLKSGEEVVLSIRPEDVELSESRRDGENVYQATVDQKVFLGEVVDFQVMIGRRS
                     VQSRAHPSLRTPVGASLYVRIDPEKCVALKASGEWAYE"
     gene            complement(12163..>12917)
                     /locus_tag="VFR57_00610"
     CDS             complement(12163..>12917)
                     /locus_tag="VFR57_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0257908.1"
                     /translation="LIVVLPFAVLLWSSFQKFYSVPSMEAIQRMTFEPYRTVLDHPGL
                     WRSVWNSLVLAVGSATLIMLVTSVICWIVVKTRLPGRWLLDNIASLPMVFPGIVLGLS
                     IMILYLYVPIGVYGTIWILVIAYVTRFLPYGLRYNTTSMLQIHKELEESAAMSGASWT
                     TSFRRIILPLLKPGLVAGWIYIMIVSIRELSSSILLYSPGNEVLSILIWELWENGQYV
                     ELSALGVMFIIALFILVMIAQWVGSKFGIKEH"
BASE COUNT         2248 a         4286 c         4131 g         2252 t
ORIGIN      
        1 cggcgcttgc cggctactca cagaagaact cgaccacgtc cgtcgcgccg gcgcgcgact
       61 gaccgaaggc aatccagccg tggccgcgca gcgtgaactc ctcgcggcgc aggatgattt
      121 ccttgtcgcc ctcgatcgtg acgaacgtcc cgttcgcgct gtggtcggtg agaacgaatt
      181 tgtcgaggcg ccgctcgatc ttgccgtgcg cgcgcgacgc catgcgctcc gtgatcacga
      241 ggtcggccga ctggtcgcgc cccatggcga gcgacgggcg caccgccgag agcacgacct
      301 cggtctcgtg catcttcagc cgcaatgtgg tgttgcgcgg cctgaacacg gccttgtgcg
      361 acgcgagcgt ggtcatgtcc tggctgtgct gccacatcag ctcgtagacc tggatctcgt
      421 gcgccttgcc cttcgccgga atggtggtga tggcgcgcgt gctggccttg agcctcggcg
      481 acatcatcaa cacggtgtcg cgtgcagtga tgatctgacc cttgctcgcg acggcggcga
      541 ggcgcgaagc cacgttcacc gcgtcgccga acacgtcgcc gtcgcgctcg accacctggc
      601 cgtagttgaa gccgatgcgg atcccgagcg tagtgctgcc gatggggggc agctcggaaa
      661 tcgcgagctg catctccatc gcggcgctgg ccgcgtcgtc cgcgctgcga aaggcggaca
      721 tcacttcgtc gccgatcgtc ttgatcacgc ggcccttcgc ggcctgcgtc ttctggcgca
      781 tgatgttgac gcagctttcc accgccgcgt gcgcgaccgc gttgcccgcg gtctcgtaga
      841 gcttggtgct gccgctgacg tcggcgaaaa gaatcgctgc ctcgctagcc tgttccgcca
      901 tccagaacgt ctgcccccgg tggtcggatt gtactctgcg cgccagcgtg ccttaattac
      961 gtgtgcgcac gccgatcacg aaaaggttgg cgaccacgat cgtcgccgcg aggaagtaga
     1021 acgcggcggc aaggccgaac ctatccgcca ccattccgcc gagcagcggg ccgagcgcgc
     1081 cgccgaccgc ctgcgctccg aacagcacgc cgatgctgga gccgcccatg tccttgggcg
     1141 tcgcttcaag catccacgcc tggatcacgg gccgcgtcgc gtagaggaag aacccgagca
     1201 cggcgaccag cgcgacaaag agcggcgagc cgccgctgag cgccatcgcc gcgagcacga
     1261 gcgcgctcgc cgccatgctt gtcatgagga tgtgcttgcg ccccatgcga tcggagaggt
     1321 ggcccgccac cggcgaggcg gcgaagccgg cagcctgcag cgcaaaaagg cacgccccga
     1381 cccagaacgg cgaatatccc atgtcattgg cgaggtagac cggcaggaag gtgagcagcg
     1441 cgctctgcgt catggtacgg aacgccgagc cggttgagag cagcacgagc gagcggctga
     1501 ggaaaagatt cttgagcccg gcgagatacg cgccgaaaga ctgcgtcttc tcttcggtct
     1561 ttttcgagct aagacgcatg ctgccgagaa atacgaacag aagcgcggcc acgacgagcc
     1621 cggggaccac gttgagcatc acgacttcgc gccaggtgaa aacggtcaac gccgcgccga
     1681 ttactaccgg cgcgacggcg tcgccgacgt taccgcccat gccgtggacc gagagcacaa
     1741 ggcccttgcg ctccggatag cgccggccga gcgtcgggat cgcggtgggg tgccagaggc
     1801 tgttgccgaa cccgaccagc gcgatgcacg cgagcagcat gacgtagctg tgagtaaaac
     1861 cgatgaggag gtagggaaag ccgatccaga agagcgacag gcccatcagc agccctttgc
     1921 ggcccacagt gtccaccagc accccgcccg gaacattcgc gaccgccgag gcgatgtact
     1981 tgcacgtcat aatgaggccg atctggctgt aggacagacc gagctccgcg ccgatgagcg
     2041 gcagcaggag atagaacgtg gctgggtacc agtgcgtgag gccgtggccg atggtgatca
     2101 gccagagctc gcgccagggc cgcggcggcg cgggatgctg cggcagtacg gccgcgctca
     2161 agccggcgtc ttttccttca cgcgcaggaa tttctccacc agctcggcgg tgagcacgac
     2221 cttcgggtca tactccggcg cgtcctcgag ctgctcgagg tcattcaggc cgcaggcctt
     2281 gtagatccgg ttggtggccg agataagtcg caccggccgc tcggcgctcg cgttgaagtg
     2341 ctggtggatc gtattcggcg gaatgtagat cacgtcgccc gcctcccact cgtagcgctt
     2401 cacctcggct tgcggcttcc agtgataggt gtcggtgatc tcgacgtcgc agtcctggtg
     2461 caggtcgtag ccgcggccct cgaggacata gaggcattcc tcggcaaggt ggcggtgctt
     2521 gcccgagcgg ctgcccggcg gaatgatcag catgtaggcg tcgaccgtct ccatgcgcgt
     2581 gttcatgccc tcgttgatga ggtgcttgag cagcccctgg cgcgacatct cccacggcat
     2641 gtcctcgggg cgcacgattt tcttccgctt ggcgttgcgc gccggcgcgg ccgcggcatc
     2701 gtccagcagc gcctgataga gcttctggtt gtgtcggccc ttcctccacg ccctcgactc
     2761 gtcccaagcc atgtcaatcc tcggagtgat tgaagttatg ctctgctggt cgcacctcgt
     2821 agccttccgc tccgggcgcc ggcgtcttcg ggcgcggctc taccagctgc tggaacagca
     2881 tgttcatgaa catgaacatc ggcttcgtct tgatcacgag cgcacgcgcc ggcttgtcgg
     2941 ggctcgcgtt gaagtgctgg tgcacgcagt tgttgtgcac gatcgcgacg tcgcccgcct
     3001 gccagtcgta gcggatgccg tcgtgaattt caaagccttt tccatccaat atataaaagg
     3061 ctgcctcgtt gacgtgcccg tgcttctgcg acttgccgcc gggcgcgtat tcctcgaggt
     3121 gcaggtggaa catctgggtg acgccgtcct tgggctcgac gtaatggcgg ctgtaggtct
     3181 gcgggccgtc gacgaacttg agctccctgc ccttgcgcac gcgcggcatc gcgcgcagcc
     3241 gcgcgagctc ctctttcagg ctgtactgcc cctgtatcgc gcggacgaaa atccggtcct
     3301 tcttgctgtg atagtgcgac tcggtgctca ttcgcgcttc agtatacatt tatccgatga
     3361 ccgtttacct cgtggtcttg ctgtcgatgc tgaactcgat cggccagcgc ggcagcaagg
     3421 tcgcgatttc gctctacgcg ctggagctcg gcgccggcgc cgccgcggtc ggcatgctgg
     3481 cggcgctgtt tgccacgttc ccgctccttc ttgccgtgca ggccggaaag atctcggacc
     3541 gcttcggcgt gcgcattccc attatttgcg gcgcgctcac catggctgtc gggctcgcgg
     3601 tcgcctacat ggtttcggac attcattggc tgttcgtctg ccccgcgctc atcgggctgg
     3661 ggcacatctt cttccacgtc tcgatccaca acctggtcgg ctcgctcggc gccgatgccg
     3721 agcgcacgcg caatttcgcg actttcgccc tcggcagctc gctcgccgcc ttcgtcgggc
     3781 cgtcgctcgc cggcttcgcc atcgacctcg ccggcttcag gccggccttt gccgtcctgg
     3841 cggcggtggc agccgtgccg gcgctgatcg tgctgctcta ccgtagcctg atcccgacac
     3901 gcgtgcgcca tgatgaggaa gagcaggcga ggagcgcttt cgacttgctg gcgatcccgg
     3961 cgctgcgccg cacgctcatc atgagcggcg tcacgctgac cggtatcgag ctcttctcct
     4021 tctatctgcc gatctacggt cgctccatcg gccttgcgcc ctcggtgatc ggcatgatcc
     4081 tgtcgagcta cgcggtcgcc gggtttatcg tgcgctcctt catgcaccgg ctcgcgcagc
     4141 gctacaccga ggtcggcgtg ctcaccggtt cgctattcgt cgcggccctc gcctatttca
     4201 tcgtgcccgg cctcgcggac ggctggctgc tggcggcggc cgcgttcgcc ctcgggctcg
     4261 cgctcggctc cgcgcagccg cttacgatca tcctcactta taaccacgcg cccgccgggc
     4321 gcgcagggga ggcgctcggc atgcgcatca tggcgaacaa ggtgacccag atcgcggttc
     4381 cgctcgcctt cggcgggttc ggcgccgcgc tcggcgccgt gcccgtgttc cttgccaccg
     4441 gcgcgttcct gcttgtggct ggattgatct cgctgaaacg gtaaactgcg ctcatatgga
     4501 acaacccggt tccgagatga ggaacaatgc gcgcggaaca gaatgaattc ctgacgcgca
     4561 ccggcccggg cacgccgatg ggcgagcttt tccgccgcta ctggctgccg gcgctgctcg
     4621 cagccgagct tcccgagcct gactgcccgc cggcgcgggt acggatcctg tcagagaagc
     4681 tgattgcgtt tcgcgacacg cagggccgcc tcggccttat cgacgagttc tgcgcgcacc
     4741 gcggcgtatc gctctggttc gggcgcaacg aggaaggcgg gctgcgctgc tcctaccacg
     4801 gctggaagta cgacgtgacc ggccagtgcc tcgagatccc ttccgagccc gacaatccga
     4861 agctgtgcca gcgcatgaag ctcaagtcct acccgctggt cgagcggggc ggcgtgctct
     4921 ggacctacat gggcccgcgc cagctgcagc cgccgctgcc ggagcacgag tgggcgatgg
     4981 ttccgcaaag ccatcgcttc gtctccaagc gctggcagga atgcaactac ctgcaggcga
     5041 tggaaggcgg gatcgactcg agccatgtgt ccttcctcca ccgcgagacc atcaaagttg
     5101 acccgatgtt caagggcgcg gcgcagagcc accagtacaa ccttcaggac ctcgcgccgc
     5161 acttcgaggt ggtggaatcg gccggcggcc tctatatcgg cgcgcggcgc aatgctggcg
     5221 acgggaagca ctattggcgc atcacgcaat gggtgatgcc gagcttcacg ctgatcccgc
     5281 cgcgcggcga ccacccgatc ggcggccact gctgggtgcc gatggatgat gagaattgct
     5341 gggcctggag cacgaaccac cacgcggccc gcgccttgac tcaagaggag cgcgaagcga
     5401 tggaagccgg caaaggcatc catgttccgg tcatccccgg cacctttcgt ccggtcgcca
     5461 acaaagacaa cgactacctg atggaccgca gggcgcaaaa ggaacgccgc acgttctcgg
     5521 gagtcgaggg cttcgcgatg caggatgcgt cggtgcagga aagcatggga ccgatccaag
     5581 atcgcaccaa agagaacctc atgccgacgg accaggggat catcatggcg cgccgccgcc
     5641 tcctcgccgc cgcccaggga ctgcaagaag gcgcgacgcc gcccgggacc gacccgcgcc
     5701 atcaacgcgt gcgctcggtg tcgatcatcc ttccccaggg cgtctcgttc agcgaggcgg
     5761 cagaagacgc actcgtcccg gagccgggat cggcgcacgc cacagtctag gaggaggacc
     5821 aatgcttgct cgaacgattg ccgttgcttt gctgctcgtt tccaccgccg ccaccgccca
     5881 gccggcgcca ggacctcacc gcagcctcta catgtaccag ggcgccgacc gcgaccagcg
     5941 catcattgag ggtgcgaaga aggaaaagca ggtggtcgtc tatacctcgc ttaacaccaa
     6001 ggactcggtg ccgatctcgc aggcgttcga gaagaagtac ggcgtcaagg tcgagctctg
     6061 gcgctcctcc agcgagaagg tgctgcagcg ggcgctcacc gaggcgcgcg ccggtcgcca
     6121 cgcggtggat gctttcgagc tgaacggacc ggagatggag gcgctctacc gcgaagggct
     6181 gctggaggag ttctggagcc cgtacttcaa gaatctcccg ccggcggcgt tcgccaagca
     6241 caagcactac gtcgccgacc gcttcaactt cttcaccatc gcctacaaca cgaagctggt
     6301 gaaaccggag gaggtgccga acagctacga agacctgctg cacccgcgct gggtgggcaa
     6361 gatcggcatc gaggcgagcg acgacgactg gttcgcttcg atcgtaaagc acatgggcga
     6421 gcagaaggga cttgctttct tccgcaagct tgctgaaatg aagccgcagg tgcgcaccgg
     6481 gcatacgctt ctcgccgagc tcgtcgccgc cggcgagatt ccgctcgttg ccacgatcta
     6541 caaccacaac gccgagcggc tgctcgtcaa cggcgcgccg atcaagtgga aggcgctcga
     6601 cccgaccttt ggtcggccga acgcggtcgc ggtgaccaag cgtgcgccgc gcccgtatgc
     6661 ggcaatgctt tttgtcgact tcatgctctc gccagagggc caggagcttc tcaagcagcg
     6721 caaccgcgtg ccggcgagca ccgcggtcga gaccaacctc aacaagttcc ccttccatat
     6781 gatcgacccg gtgatcgtgc tggatgagca ggcgaagtgg gaaaagatct ggtcggagct
     6841 cttcctcaaa ggccaggcga tcaagcgcga ggccgactga aatgggcaag gagtcgcact
     6901 attcgagcaa gaaggaccgg gtcttcgtcc gggcgattca gggccagtac agcctaaagg
     6961 acgagctcaa gcgcctgcgc tccctgcccc gtgtcatccg gggcaaggag atgaagttcc
     7021 aggacggccc gcagtccttt tccaagcact tcgtcgagcc ggtcgacggg atgacgcaga
     7081 cgctgcacat ccacctagag gagtacgccc ccggcggccg cacgcagaag cacggccacg
     7141 tcaacgaggc ggcgttctac atcctggacg gcaagggcta cgagatccac gacggcgtgc
     7201 gctacgactg gcaggccggc gacgtcgcga tcgtgcacaa caactgcgtg caccagcact
     7261 tcaacgccga cccggacaag cctgcgcgcg cgctcgtgct gaaatcgaag cccatgttca
     7321 tgttcatgaa catgctgttc cagcagtcgg tgatcccgcg gcccaccgag ccggcgacgc
     7381 cggccggcgc gggcttcgag ccgcggcacg acgagacgca ctacaaccac gaatgaagtc
     7441 cgccgtcgtc tcgcaggatt tcgccgccaa gttcgcgacc gaaagggaga ctccgtacac
     7501 gcgctgggtg cgcgaggaag ggctcgacat catcggctcg ttctacgtcc agaacctcca
     7561 cacggtcgag ctcaaaccct ggccgcggcg cggcggcaag ggggtgttcc tcaatcacga
     7621 cgcctcgcgc acctccaacg actgctacgt gtgcgagatt ccgcccggca agagcctcgc
     7681 gccgcagcgc cagctcttcg aggagatgat ctacgtgctc gacgggcgcg gctcgaccgc
     7741 ggtgtggaac aacgctggca accgcgcgag cttcgagtgg aaggcaggag cgatcttcgc
     7801 gattccgctc aactgctggc atcagcactt caacggctcg ggcaaggacc cggtgcgcta
     7861 cgtcgccgtg actaacgcgc cggtgatcat caacgcgttc ggcgacctcg acttcgtctt
     7921 caactgcgat cacgacttca aggagcgctt cgcaggcgag cccgagtact tcgcgaacaa
     7981 gggcgagcag aaagggctcc tgctcgacac caacttcgtc gccgacgcga tcaacctgcc
     8041 gcttttcgcc gccaaggagc gaggcgcagg cggcggccac acacgcttca gcatggcgaa
     8101 gggctcgatg aacagccaca tctcccagtt tcccgtcggc acctacaaga aggcacacgc
     8161 ccacgggccc ggcgcgcacg tcatcgtgct ttccggcgaa ggcttcagcc tgatgtggcc
     8221 cgagggcgag gcgccgaagc gctacgactg gcgcgaaggc acgatgatcg ttccgccgaa
     8281 cctgtggttc caccagcact tcaacaccgg caccacgccg gcgcgctacc tcgccttcaa
     8341 ggctgagggc gtagccgtgc gcaacgcgca gggcgtgccc aaggcctgga tcagccgtcg
     8401 cctcggtggc gaccagatcg actacgccga cgagcggccc gagatccggc agtggttcgc
     8461 cgaggcgctc gcgaagcatg gcgtcaagcc gaacatggac aaggcctacg ccatggagcg
     8521 cggcgagctg ccagaaaaag ccgcttagta cttgtcgagc cggaagcggc gcggcttgag
     8581 cgcgcggcag atctcccgcg tgagccctgc ctgcggcagc gcgagaccgc actccgcggc
     8641 catctgcgcg acgatcgcca tgtcatcctc ggcccacgcc atggtctgcg taccccactt
     8701 ctcgagcgcc gagttcgccg cgctcgacat gccgagcgct cggcgcagca tctggacatc
     8761 tacgccatag cgctgcgcca gagcgaggcc ttcgtggtcc gctaccaggc aggcccacat
     8821 gatgaggttg ttcaccgcct tcgcgacctg cgccgagccg acagcgccag tgtggactac
     8881 ctcggatgaa taggccttca gcaccggagt gacgcgctcg accacctcct tccgtccgcc
     8941 cacgaacgaa agcagcgtcc cctggtccgc cgcctcccct ccgcggcaaa ccgtcgagtc
     9001 gatcagcgaa atctcccggt tggctttggc cagctcttgc acggttttcg gcaggattgt
     9061 gctcaggata gcgacgatct tccccttcaa gggctcgcgg tttttctcga gcagctggcg
     9121 cacctcgtga tcgaagccga cgcagacaag aacaatgtcg ctctctcttg ccaagacagc
     9181 ggcatcgctc cgccacgacg cagacttctt ttccacctca cccttctttc ctgggtcaac
     9241 gtcggtcgca agagtccgaa acccttttct tgccaagtgc ccgatgatcg gcagccccat
     9301 gcgccctgct ccgatgacgc cgacggtgtc gctcatcgtt tctgggtaat cgtgtctagg
     9361 agcgccggct ggccggcctt gacgcgcgcg atggcgcgcc tgagcgcggc ggcgacatcg
     9421 ccgggacggc cgataggacc ctcggcgtac cagcccatgg acttcgccag cgcggcgaaa
     9481 tccggggccg ggtcgtccat gtccatgccg atgtgcgcgc gctccgccgg cgtgccgcgc
     9541 agcttcgcca tgcggatctg gtgctcccag tcgttgtaat aggcgcggtt gttgtacatc
     9601 accacgagca gcggaatgcg gtgctttgcc gccacccaca gcgcgcctgc gtcgaacatg
     9661 aggtcgccgt cgggctgaag gtcgacgacc agccgctttg catcgcggtg cgcgagcgcg
     9721 acgccaagcg aaattccgat ctgcgtcgcc gtgccgagag actttccggc atggcgatac
     9781 ggcttgtcga agtcccagat ctgccgcgcc cggtcgtcca gggtccccgc cgcgagcacc
     9841 cagtcttcgg acttgatcgc gttccacact tccgtcgcga gccgcggcaa ggtaatcggg
     9901 ctcgcgtccc agtcctcctt cgcttccgcc tgccacttcg catgcgcttt ttggcgcagc
     9961 tcggccacgg ttcttagcca ggaatcgcgc ttaacgggtc ttgatgcaag aagcttcgtt
    10021 agcgtaggaa tcgcaagcgt ggtatccgcg agaatgcgca ggtcggcgtg cgcaaggcgc
    10081 tggtagtcca tcgcccagct cgaaatctcg aggtcgccga agccgatgtc gatccatttg
    10141 cagtccgccg gaacgagcga ggtcacctcg cgcgtcgtgc tcacgagctc ggtcgtgggg
    10201 cgctcccagt cccgcgtatc gaggcaaagg atgaggtccg cgtcgcgaaa gacatgcttc
    10261 accatgctca ggttgagcgg atgccggctc gggaagttga ggcggctgtt gacgtcgtag
    10321 accggtgcgc cgagcgtctc ggcgagctcg accagggcat ggaagccagc gggctcgcgg
    10381 ccgacatact cggcgatgat cgccgggcgt ttcgcggcag caagcatctc ggccgctctt
    10441 tccagcgctg cggggtccgg tgcgagcggg ctcggcactt tggcggcgga cgattttgga
    10501 agcgcgacct catgctcgag cggctgctcc tggagccagg cgtcgtagca catgtagatc
    10561 gggccgcgcg gctcggtcat catcaccgca taggcgcgcg cgaaagattc gggcacgccg
    10621 tcgatcacgg tcggctgata gtcccacttg gtgtatgcgc gcaccgcctc gccctgcacc
    10681 agcgcgctgt ggatccagtc gatgcgcggc cggcgtttgg tctcgtccat aggccccgtg
    10741 gcgccgatga tgaacaccgg cgcgcggtcg gcgtaggcgt agtagatcgc catgttggcg
    10801 tgcaggaggc cgaccaggtt atgcaggatg gcgaccatcg gtcttccgct cgccttggcg
    10861 tagccgtgag cgatctgcac cgcggtctcc tcatgctggc agagcatcat ggccggccgg
    10921 ttgccgccgt aattcaccat cgagtcgtgc agcccgcggt aggaggcgcc cgggttgagt
    10981 gccgcatagg gaaaatcgta ggcatgcagc atgtccacga tcacgtcgga agcccagcgc
    11041 ttcattcgta agcccactcc ccggacgcct tgagcgccac gcacttctcc ggatcgatgc
    11101 gcacgtagag gctcgcgccg accggcgtgc gcagcgacgg atgcgcgcgc gactgcaccg
    11161 agcggcggcc gatcattacc tggaagtcaa cgacctcgcc gaggaatacc ttctggtcca
    11221 cggtggcctg gtagacgttc tcgccgtcgc ggcggctctc ggaaagctcc acgtcttccg
    11281 gccggatcga gagcaccacc tcctcgccgc tcttcagcga atcgacaccg cgcgccttca
    11341 gttcgccgat ctcggagcgg atgcggtaga agccgcccgc cgcatcggct gcgctcacgt
    11401 cacccttgat gaagttggtg gtgccgacga agtctgcgac gaactggttg cgcgggcgct
    11461 cgtacacctc gcgcggcgag ccgagctgct cgatgcgccc cgcgttcatc accgcgatcg
    11521 agtgcgacag cgcgagcgcc tcgctctggt cgtgcgtgac gtagacggtg gtgatgccga
    11581 gctcgcgctg cagccgcttg agctcgaagc gcatgcgctc gcgcagcttg gcgtcgaggt
    11641 tggaaagcgg ctcgtcgagg agcaggagcg acggctccat gacaagggcc ctcgcgagcg
    11701 ccaggcgctg ctgctgcccg ccggagagct tggtcgcctc gcgatcggcc atttcctcca
    11761 gcgccaccac cttgagcacg cggtgcacgc gctcgtggat ctggattttc ggcatgcttt
    11821 tcctggaaac ctgcaacgga aaggccgcgt tgtcgaacac cgtcatgtgg ggccaaatgg
    11881 cataggactg gaacaccatg ccgagtccgc ggcggttcgg cggcacgaac acccgctgtg
    11941 tcgaggaata gatgacgcgg cccccgacga cgatttcccc ggaacgcggc ttttccagcc
    12001 cggcgatcga gcgcagcgta gtggtcttgc cgcatccgct cgggccgagc agcgtgaaga
    12061 gcttgccctg cggcacgtcg aagctcacgt cctgcgcggc gcggattgtc tggccgtgct
    12121 cgccggcgta ctccgtgcac agagccgaaa cgctgagcaa gcctagtgct ccttgatccc
    12181 gaacttgctg ccgacccact gcgcgatcat caccaggatg aacagcgcga tgatgaacat
    12241 caccccgagc gccgagagct cgacgtactg gccgttctcc cacagctccc agatgaggat
    12301 cgagagcacc tcgttgcccg ggctgtagag aaggatcgag ctcgagagct cgcggatcga
    12361 cacgatcatg atgtagatcc agccggcgac cagccccggt ttgagcagcg gcagaatgat
    12421 gcgcctgaag ctcgtcgtcc aggaagcgcc gctcatggcc gccgattcct cgagctcctt
    12481 gtggatctgc agcatcgagg tcgtgttgta gcgcagcccg tagggcagga agcgcgtcac
    12541 gtaggcgatg accaggatcc agatcgtgcc gtagacgccg atcggcacgt agaggtagag
    12601 gatcatgatc gacaggccaa gcacgatgcc cggaaacacc atcggcagcg aggcgatgtt
    12661 gtcgagcagc cagcggccgg gcagccgggt cttgaccacg atccagcaga tcaccgaggt
    12721 cacgagcatg atgagcgtgg ctgagccgac agcgagcacc aggctgttcc acaccgagcg
    12781 ccacagtccc gggtgatcga gcacggtgcg gtagggctcg aacgtcatgc gctggatcgc
    12841 ctccatcgaa ggcaccgagt agaacttctg gaacgaggac cacagcagca ccgcgaaagg
    12901 cagcacgacg atgagca
//
LOCUS       DASTPD010000017        23246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_618638, whole genome shotgun sequence.
ACCESSION   DASTPD010000017 DASTPD010000000
VERSION     DASTPD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23246
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618638"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFR57_00615"
     CDS             <1..431
                     /locus_tag="VFR57_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0257909.1"
                     /translation="RAHIAYYGVGVLFCGDTLFACGCGRVFEGTPEQMYASLEKLRAL
                     PDDTKVYCGHEYTLANIGFAKGVEPQNSALTARERRDRELRAAGRPTVPSTMREEKAT
                     NPFLRCTEPAVVDSVNKYLGQRVSDPVRVFAAIREWKNRS"
     gene            413..1867
                     /locus_tag="VFR57_00620"
     CDS             413..1867
                     /locus_tag="VFR57_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0257910.1"
                     /translation="MEKPIVKNLAFALVLALAACATPPAPDSVPGAPVPLPPAVPAPP
                     PLPVAVAPTPPKPPPAPKPEPEKPAAEKPAPLPAVTVVKPSPPPPPVEVDAGDLWQRI
                     RNGFAIPDLKGTLVTQKQRQYAARPEYLQRIIERSKLYLYHIVEEIEKRGMPTELALL
                     PMVESAFNPMAYSRAHASGLWQFIPGTGRRFELEQNSWYDGRRDIVDSTTAALDYLTK
                     LHEMYGDWHLALASYNWGENAVRRAIANNKAKRKKTDYASLRMPKETRHYIPKLQALE
                     NIIADPQKFGVDLDAIPNQPYFAVVTELPDIDVHVAAKLSEMSVQEFIALNPAFSRPL
                     IRSSATRRLILPADKLVAFYDNLDQLDESELVSWQVYEPKPGEKLSDIAQRFHMSEAQ
                     LRRVNGIPHNATRIPKELVVPKSGEATATLADLPLMYAPPIPQYGPARAVHVVKAGDT
                     LGSIAKRYRTTVASLKAGNRLGPYLQVGQKIYIR"
     gene            1870..3297
                     /locus_tag="VFR57_00625"
     CDS             1870..3297
                     /locus_tag="VFR57_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0257911.1"
                     /translation="MKRFRLALLAVLVSACASAPQPPEVVPESLPAPTVARADKPSTP
                     PPFRAEAHEIKDRLREMAPIDRTAPIDDLWQRVRYGFAMPDLDNALVRTQTRYYATRP
                     DYLQRIFDRGRLYLYHIVEEIERRGLPTELALLPMVESAFNPMAYSRAHASGLWQFIP
                     GTGKRFELEQNWWYDARRDIVDSTNAALDYLEKLYEMHGDWQLALASYNWGENAVARA
                     IAKNRAAGKPTDYESLAMPSETRNYLPKLQALKNIIANPEPFGIDIGPIPNQPYFATV
                     TKLRDIDVQLAARLAEMPVEEFIALNPGFSRPVIRASVTPRIVLPADKVDVFHDNLER
                     LGEKSLVSWKTYYPKSEETFDSIAKKHAMSVAQLKEVNGIAARQKQVPRLLVVPTKDA
                     TVQKLPIMYAPPIPVTMRRVFHTVQPGETLASIAKKHRVSEEDLKRWNGVSKAVPGRK
                     LALEVRATPKAKPRAKAKTKASTRT"
     gene            complement(3294..4871)
                     /locus_tag="VFR57_00630"
     CDS             complement(3294..4871)
                     /locus_tag="VFR57_00630"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0257912.1"
                     /translation="MSLLEVRSLSVHFSARKVVDQVSFTLDAGEKLALVGESASGKTV
                     TALSTLRLIENARLTGEILFDGRSVLAMPPDELHQLRGRDIAVIFQEPMTALNPIYTV
                     GDQIVESLELHTGLRGTAAWKEAVEAMRRVGIDEPERRALSYPHQLSGGQRQRAMIAM
                     ALACNPKLLIADEPTTALDVTIRLQILELLERLRAEARMALLLITHDLNLVRRFADRV
                     AVMEKGVLVEQGTTAQVIGRPQHPYTQKLVNSRPARDVAAPAAGQVVQAKKVQVHYPT
                     RLPGIRGWFKSGRFTAVDSVDFDLAPGETLGVIGESGSGKTTLALAVLGLMQAEGDIR
                     IGGRTWAARAERRNMRKEIQVVFQDPLSSLSPRRTVEQIIGEGLEIHEPALDANARRE
                     RVVQALYEVGLTEGGRAEPLLARYPHEFSGGQRQRIAIARALIVRPKVVVLDEPTSAL
                     DVTIQKQVLELLASLQKKYGLSYILVTHDIDVVRAMAHRVMVMKDSKVVESGTLEQVV
                     ERSKSEYTRRLVEAGYA"
     gene            complement(4868..5953)
                     /locus_tag="VFR57_00635"
     CDS             complement(4868..5953)
                     /locus_tag="VFR57_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257913.1"
                     /translation="MSLGSTSATQDAVHVSPTLRAWLRFRSNRLGLASLIVLGVLFLI
                     SLLAEVVSNDRPLAVRYQGAWYFPVFQDLPETTFGGQFPTRTDFHDPQIKANLAAPGN
                     FAVFPINRHHYKTVNYFAKAPAPAPPSAENLLGTDDKGRDLLARLLYGFRVSLVFAIL
                     VAGLCTILGVLYGAIQGYFAGWTDIGMERFTEIWGSLPQLYMLIIFSAIFYPSFGLLV
                     VLLSMFGWLGLANYIRAEFLRNRTLDYVRAARALGQSNRKIIWRHILPNSLTPVVTLL
                     PFEMTAAIGTLTALDFLGLGVPPGMPSLGELLNQAKENLDAWWIAVTTFGVLVVMLML
                     LVLIGDALRDALDPRKVLAGDESGLRR"
     gene            complement(5983..7032)
                     /gene="yejB"
                     /locus_tag="VFR57_00640"
     CDS             complement(5983..7032)
                     /gene="yejB"
                     /locus_tag="VFR57_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEU0257914.1"
                     /translation="MWSYILRRLLLMVPTLLGILVITFVIVQFVPGGPVEQMVAQLEG
                     RNLGAGEVGAPSGDSGYRGRQGLDPKQIEEIRKLYGFDKPAHERFFQMLGQFARFDLG
                     RSFIHNKSVWELIKEKLPVSISLGMWTFVLIYLISVPLGIAKAVRAGTPFDTATTFLI
                     LLGYSIPSFVLGVVLLVYFGGGTFVQWFPLRGIVSLNWDQLSLWGKITDYLWHITLPV
                     TAMVVGGFAVITILTKNSMLEEIRKQYVMTARAKGVAQHRILWRHVFRNALIPIMTGF
                     PAAFIGAFFGGSLLIETLFSLDGLGLLSYESVIRRDYPVVFGSLYIFTIIGLLTTLIR
                     DLSYVWVDPRVKFFD"
     gene            complement(7036..8823)
                     /locus_tag="VFR57_00645"
     CDS             complement(7036..8823)
                     /locus_tag="VFR57_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0257915.1"
                     /translation="MKVFLAAVVFLCAKPVWAAHAYSQFGDIKYPPGFTHFEWVNPNA
                     PRGGEIELVPPLRITNFDKFNPFTIRGSSPPGLGALVFESLLTGTLDEPTTAYGLLAE
                     DVALAPDGLSVTFRLNAKARFQDGTPVSAADVKHSFDTLMSKQASPAYRFALADVKQA
                     VVTGPLSVRFDFRNAGPELPLMVGGLPVFSRAWGAGKQFDEVISEHPIASGPYKIGRM
                     NFGRDITYERDPNYWARDLNVRRGMFNFDRITYKIYKDNTAQTEAFRAGEFDYIQVFS
                     ARDWARRYTGKKFDSGELIRGEWRSKNAGDFQGFIINTRREKFKDPRVREALSLAFDF
                     EWQNRQMMYNSYTRVRGFFNNSDFEAKGLPGADELKLLEPLRKTLPAKVFSQDVPLPP
                     STNPPGSLRANLLKAKALLEEAGWTYRDGALRNAKDEALVVEYLDGGSFERVFVPYAQ
                     ALERLGIEGRYRRADFALIQKRLQVFDFDLFSMRVPGQEAPGAELLSRFGSHSADTPG
                     SSNLIGVKDPGVDALLEKVVSSTTRPDLVASLRALDRVLRHSHYVIPHWYAANFRVSY
                     RAGRFEQPAVAPQYYGAEDWVLRTWWRKK"
     gene            complement(8830..10614)
                     /locus_tag="VFR57_00650"
     CDS             complement(8830..10614)
                     /locus_tag="VFR57_00650"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0257916.1"
                     /translation="MLDTAGGAVRAVDGIDFELRKGECFALVGESGSGKSMTALSVMR
                     LLPEAGRITSGRVGLDGIDLLGLPEAAMRAVRGRRVAMVFQEPSTALNPVLTVGLQIV
                     EVIERHKSSSSLAARDNALELLRSVGIQDAERRFDEYPFQLSGGLKQRAMIAAALAVD
                     PEVLIADEPTTALDVTIQAQILDLLARLQAERGMALLLITHDLGIVARMAQRIAVMYA
                     GEIVELAERDLFLRAPQHPYSQKLFAALPTPQRRAGDLAVIRGQVPPLTKKFDHCRFA
                     DRCDYVFERCRVEAPRLLEPAPHHFARCHLREESLAPPRSTSDSAGEGTVRSEASSGI
                     LLQAKDLKVHFPIRKGIFRRTVGHVRAVDGVSLELREGRTLALVGESGSGKTTAGKAI
                     LQLIAPTGGSVRFKDVELTGLSRRDLRPRRGAMQIIFQDPYGSLNPRLRVAEILLEGM
                     RSLGIGANDLERTRSLQNLLADVGLPANALERYPHEFSGGQRQRIAIARALAVNPKLI
                     VCDEPTSALDVSVQAQILNLLRALQQRLGIAYLFITHNIAVVDYLAHEVAVMYQGKIV
                     ECGAVDDVLRRPQHPYTKQLLAAVPTID"
     gene            complement(10641..11975)
                     /locus_tag="VFR57_00655"
     CDS             complement(10641..11975)
                     /locus_tag="VFR57_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257917.1"
                     /translation="MTPVILATDALLWLLVAAVVVYGLYCRRRPHLAAPWKRVFQSKA
                     AVVSGVVLGCYLLVGLADSLHYRAAGSVEVLSLLDRTLAMLRQKSEKTYSAPLATRLY
                     SKEQIETPDGKQVRDFPRLRHGGSHLQQELDRWSDVGLRAAAGLAAAAVLWALLFFIS
                     RPLARRYPHVPFGAVWITLAVILGLAGPIASLAPHYHVLGTNQVGQDVLYLALKSIRT
                     SLAIGTLTTLVLLPLGIALGIMAGYFKGWIDDAIQYLYTTLNSIPGVLLIAAAVLMMQ
                     VVIDRHAEWFPTAAQRADFRLLVLCAILGLTSWTGLARLLRGEALKLSQLEYIQAAHA
                     FGVSDARILGRHILPNVSHIVIIAMVMDFSGLVLAEAVLSYVGVGVDPATTSFGTMIN
                     SARLEMGREPIVWWSLAAAFVFMFVLVLAANLLADAVRDGFDPRVRVGALAV"
     gene            complement(11972..12949)
                     /locus_tag="VFR57_00660"
     CDS             complement(11972..12949)
                     /locus_tag="VFR57_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257918.1"
                     /translation="MLAYIVRRVLYAIPILIGVNLITFVLFFVVNTPDDMARMHLGAK
                     RVTPEAIEKWKQERGYDRDLLWNEKAPGLGKVTDTIFFDKSVKLFAFDFGRAEDRRDI
                     AHEIRTRMGPSLALAIPVFLVGLAVNITFALLMAFFRATYLDFWGVVLCVAMMSISTL
                     FYIIGGQYMASKILHLVPISGYDGGFDAAKFVILPVLIGVVSGIGSGSRWYRTIFLEE
                     MGRDYVRTARAKGLAESVVLFRHVLKNAMIPILTGAVVVIPLLFMGSLLAESFFGIPG
                     LGSYTIDAIQAQDFAVVRSMVFIGSVLYIVGLLLTDLSYTVVDPRIRLE"
     gene            complement(12949..15024)
                     /locus_tag="VFR57_00665"
     CDS             complement(12949..15024)
                     /locus_tag="VFR57_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0257919.1"
                     /translation="MRILLMLMLFVAGCGAVWNDPYPRSERGANILYSAFVNRPKHLD
                     PVQSYTVDESLFTRQVYEPPLHYHYLKRPYELIPLTAAEVPKPKEIDGGKFTVYEIRI
                     RPGIRYQPHPAFVEGNQALSASEIKKLKTPYDLPLGTRELVAADYVYQIKRLAHPRLH
                     SPIYGLMAEYVVGLPEFADKLKKLNRPGWIDLREHPLAGVEEVDRYTYRVTLKGRYPQ
                     FVYWLAMPFFSPIPWEAEKLFHQPGMELKNFTLDWWPVGTGPYMLRENDPNARMVLER
                     NPNYRGMPYPAEGESSDGEAGLLKDAGKTMPFIDRVIFSREKESVPYWNKFLQGYYDS
                     AGISPENFDHAVRVAVDGGAEPSPEMLERGIELSTAVAATVFYFGFNMKDPVVGGNTE
                     RARKLRHAISIAVDMEEYLSIFLNGRGIVAHSPVPPGIFGAREGREAMNPITHVWRDG
                     REQRRALLEAKKLLAEAGYPEGTDAKTGAPLVLYFDTTSRGPGDKARLDWWRKQFEKL
                     SIQLEVRETDWNRFQEKIRKGTQQVYGLGWHADYPDPENFLFLLHGPQARADGAGSNS
                     SNYTSAEFDALFERMKSMSNSPERQQIIDRMVEIARRDAPWIFGFHPKDYTLRHQWLA
                     NVKPNDMAGNTMQYLRVDPQRRAEFRRQWNRPVVWPVLLLLLALAISIVPAVRTYRKR
                     ERMAARPAA"
     gene            15046..15843
                     /gene="fabI"
                     /locus_tag="VFR57_00670"
     CDS             15046..15843
                     /gene="fabI"
                     /locus_tag="VFR57_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU0257920.1"
                     /translation="MALLSGKKILVTGVLSNRSIAYGIAKAARREGAEIALTYQNERF
                     KDRVTDMAKEFGSSLALPCEVTSDAEIESLFGGIRKNWGGLDGLVHAIAFAPREAIAG
                     DFLDGVSREAFRQAHDISVYSFPALAKAALPLLSKNASLLTLTYLGAARVVPNYNTMG
                     LAKASLEASVRYLAASLGPRGIRVNGISAGPIKTLAAAGIAGFGKILKFVEEHAPLRR
                     NVTTEDVGNAAAFLLSDLAAAVTGEILYVDAGFSHVVAGIPATSTEA"
     gene            complement(15856..17706)
                     /locus_tag="VFR57_00675"
     CDS             complement(15856..17706)
                     /locus_tag="VFR57_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0257921.1"
                     /translation="MFDLVTKNKRVIQVFLALIAITFATWGIESYTQFRSSRDTVASV
                     DGMEISRREFAEELRRQQEQMRRMFGGAIDPALLDTPESRVAVLEGLISQRLLAREAA
                     RGHMIMSREAVIDAITSAPEFQEDGKFSTAKYSAYLSSRGISDQGNVAELQSQIPLAR
                     VAGAISETAIAPRSVAARLAALEAQKREIAEVRISEKQFLPQVKIDEAQVKAHYDANQ
                     ADYRTPERVRAEYVVLSAEALSRQDPPTEAEIKASYDSRANQFRVEEQRRASHILVKT
                     KDEAEKILQEAKKAPARFAELAKKHSQDPGSAEKGGDLGWFGRGMMVKPFEEAVFKLG
                     QNEMQVVESEFGYHVIRLTGVQPGKTRPYEEVKKELTDELARVKGQRRFAEAAETFSN
                     LVYEQSDTLKPAAERFKLPVQQTGWISKSARQELGALDNPKLLAALFTSDAIKNRRNT
                     DAIEVAPNTLVAARITDHQPAAQRSFEEMKGEIAEQLRRREASALALKEGTAKLEQLR
                     KGQDAGLKWSAPRVVSRREAQGLSGNMLRQVMSADTAKLPVYIGVPLPDAGYALIRIS
                     KVIDEPVKEGDPQVSARAAQLFGGAQYEAYIASLRAQADVEINQKNLEAK"
     gene            17800..18318
                     /locus_tag="VFR57_00680"
     CDS             17800..18318
                     /locus_tag="VFR57_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0257922.1"
                     /translation="MTRFLPLPFLIALFVACAQARDVPYVPTPEKVVERMLEVAKVGP
                     NDVVYDLGSGDGRIVIAAAKNHGARGVGIDIDPERVREARDNASRAGVANRVEFREGN
                     LFDANISEATVVTIYLLSGINMKLRPKLLAELKPGTRIVSHAFDMGDWKPEATAKVGT
                     STVYYWVVPPRK"
     gene            complement(18359..18517)
                     /locus_tag="VFR57_00685"
     CDS             complement(18359..18517)
                     /locus_tag="VFR57_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257923.1"
                     /translation="MSNPKQAAQGSAATAQRTLKFDEMRPLQKVKFVLKVAVCMISFG
                     FIYPHILD"
     gene            18671..19477
                     /locus_tag="VFR57_00690"
     CDS             18671..19477
                     /locus_tag="VFR57_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0257924.1"
                     /translation="MAAKQLKTVLFAEVSGRDEYVRAAGRVIGWNGISDCVNSLQQTA
                     RSSGGKVVKVIGPELMMLFESPDAAANAASRMHAAVNSLPTVGGAKLSVKVGYHAGPV
                     IEGSDDLLGDTVQQVAALLRQAHAGETITTPQTARLLNPSFSGQVTVPGSAGGIHRTR
                     AQRMLRLTYGSESATCGPDNRIVLVGRDPACALVTPTAFASRRHCTIVLAADGLKVRD
                     HSSLGTFLTNEGSEEIQLANGQEVALGTHGWISVGCARAFASRLLEFTGQ"
     gene            complement(19510..19758)
                     /locus_tag="VFR57_00695"
     CDS             complement(19510..19758)
                     /locus_tag="VFR57_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257925.1"
                     /translation="MFDADAPTRYHQGGVSGLYAWGPDLPRHFIDTGMSPRRVANVVR
                     GLRSREEFTLARLLAAKRAYGAKARPWAGRPFRKPRPD"
     gene            complement(19976..20491)
                     /locus_tag="VFR57_00700"
     CDS             complement(19976..20491)
                     /locus_tag="VFR57_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0257926.1"
                     /translation="MSAVGERNNFGLAPQERYRILTQYRTIAMVGLSANYYNASSFAA
                     IYLDANGYDIVPVNPTQAGKRAILNRPVYADLAAAAAAHPRPIEIVDVFRPAREAPAI
                     ARQAVQIGAKVLWLQLGVISEEAAQIARDAGVQVVMDRCCKIEHARFFGGLRTIGLNT
                     GVVTARLALRI"
     gene            complement(20488..21879)
                     /locus_tag="VFR57_00705"
     CDS             complement(20488..21879)
                     /locus_tag="VFR57_00705"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEU0257927.1"
                     /translation="MKRERVPNESQFGFDTLAVHAGQRPDPVTGSRAVPIYQTGAYVF
                     EDTDHAANLFALQRFGNIYSRIMNPTVAVFEERIAALENGIGAVATASGQAAQHLALF
                     TLMQAGDEFVASRSLYGGSYQQFDVSFRKVGIQARFVDGSDLEQWKKAVTPKTKAFYA
                     EVMGNPTIDIVDIEPLAALAHEVGVPLIIDNTFASPYLCRPLEWGADIVLHSATKFIC
                     GHGTAIGGVIVDGGRFPWDNGKFPGMTEPSKGYHDLRFFDYFGDFSWLLKCRAEMLRD
                     YGPTMAPFTAWLMLQGLETLHVRMARHVENAQKVAEFLESHRAVEYVNYPGLASNQYH
                     ALARKYLPKGAGSIFTFGIKGGREAGQKFIESVQLFSHLANVGDAKSLVIHPASTTHQ
                     QLSDDELRAARIGPELVRVSIGIEDVADIVSDLDQALAASQKAGPPAGRAKVEQGVGH
                     SILSKAYGAPYQS"
     gene            complement(21876..22832)
                     /locus_tag="VFR57_00710"
     CDS             complement(21876..22832)
                     /locus_tag="VFR57_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0257928.1"
                     /translation="MQQDDEARLDALLVELLGGVDEPIILARVLSPARPGCTVQDYSY
                     SSRAFMKRTRRILPCSSHAVLLVVALWAGLVNAAEIRVMTSGGFTAAYNALTPQYEKL
                     TGDKIVTMYGASMGGAPDSIPSRLARGEPVDIVILAAPALDELVKQGKVVPGSRVDLV
                     RSTIGMSVRAGAPKPDISSVEALKRTLLEAKSIAYSASASGVYLSTNLFPRLGIWDAI
                     KDKSKRIESERVGTIVARGEAELGFQQVSELLPIPGIDYVGPLPAEVQQVTIFSAGIA
                     VGAKEPEAAKALIKYFTSADAAPVIAKTGLEPISQAEARKAP"
     gene            22827..23093
                     /locus_tag="VFR57_00715"
     CDS             22827..23093
                     /locus_tag="VFR57_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEU0257929.1"
                     /translation="MHAQATQFAARTLAGTKFKEPEGDDFRAAQDLLRRFAALGGRTE
                     VCGIAMDRCAIARDNLIECVVVERNVFVNSVALQNKGYAYMPIF"
     gene            complement(23108..23184)
                     /locus_tag="VFR57_00720"
     tRNA            complement(23108..23184)
                     /locus_tag="VFR57_00720"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23148..23150),aa:Asp,seq:gtc)
BASE COUNT         4195 a         7671 c         7434 g         3946 t
ORIGIN      
        1 cgcgcgcgca tattgcctat tatggggtag gtgtgctttt ctgcggcgac acgctgttcg
       61 cctgcggctg cgggcgcgta ttcgagggca cgcccgagca gatgtacgcc tcgctcgaga
      121 agctgcgcgc cctgcccgac gacaccaagg tgtactgcgg ccacgagtac acgctcgcga
      181 acatcggctt cgcgaaaggc gtagagccgc aaaacagcgc gctcaccgcg cgcgagcgcc
      241 gcgaccgcga attgcgcgcg gccggcaggc cgaccgtgcc gagcacgatg cgcgaggaaa
      301 aggcgaccaa tcctttcctg cgctgcaccg agcccgcggt cgtcgactcg gtcaacaagt
      361 acctgggcca gcgcgtttct gatccagtgc gcgtgttcgc tgccatccgg gagtggaaaa
      421 accgatcgtg aagaatctcg cgttcgcgct cgtccttgcg ctcgcggcgt gcgcaacgcc
      481 tccggcaccg gattcggtgc ccggggcgcc ggtcccgctc ccacccgcgg tcccggcgcc
      541 tcccccgctg ccggtcgctg ttgcacccac gcctccgaag ccccctcccg caccgaaacc
      601 cgagccggag aagcccgccg cggaaaaacc ggcgccgctg ccggccgtca cggtggtcaa
      661 gccctccccg ccgccacccc cggtcgaggt cgacgcgggc gacctctggc aacggattcg
      721 caacggattc gcgattccgg atctcaaggg cacgctcgtc acgcagaagc agcgccagta
      781 cgccgcgcgg ccggagtacc tgcagcgcat catcgagcgc agcaagcttt acctctatca
      841 catcgtcgag gagatcgaga aacgcggcat gcccacagag ctcgctctcc tgccgatggt
      901 tgagagcgcg ttcaacccaa tggcgtactc gcgcgctcat gcctcggggc tctggcagtt
      961 catcccgggc acgggaaggc gcttcgagct ggagcagaac agctggtacg acgggcggcg
     1021 cgatatcgtc gactcgacga cggctgcgct cgactacctc accaagctgc acgagatgta
     1081 cggagactgg cacctcgcgc tggcctcgta caactggggc gagaacgcgg tgcgccgcgc
     1141 gatcgcgaac aacaaggcaa agcgcaagaa aaccgactac gcgagcctgc gcatgcccaa
     1201 ggagacgcgg cactacatcc cgaagctgca ggcgctggag aacatcatcg ccgacccgca
     1261 gaagttcggc gtcgaccttg acgcgattcc gaaccagcct tacttcgcgg tggtcaccga
     1321 gctgcccgac atcgacgtgc acgtcgcggc aaagctgtcg gaaatgtcgg tgcaggaatt
     1381 catcgcgctc aaccccgcct ttagccgccc gctcatccgc tcctccgcca cccgacgcct
     1441 catcctcccc gcggacaagc tggtcgcgtt ctacgacaac ctcgaccagc tcgacgagtc
     1501 cgagctcgtg tcctggcagg tctacgagcc gaagcccggc gagaaactgt ccgatattgc
     1561 ccagcgcttt catatgagcg aggcgcagct gcggcgcgtg aatggcattc cgcacaacgc
     1621 cacgcgcata cccaaggagc tcgtcgtccc gaagagcggc gaggccacgg cgacgctcgc
     1681 cgacctgccg ctcatgtacg cgccgccgat cccgcaatac ggcccggcgc gcgccgtaca
     1741 cgtggtcaag gccggagaca cgctcggctc gatcgccaag cgttaccgca cgacggttgc
     1801 aagcctaaaa gctggcaacc ggctcgggcc ttacctgcag gtcggacaaa agatctatat
     1861 aaggtaggcg tgaagcggtt tcggctcgcg ctcctcgcag tgctcgtcag cgcctgcgcc
     1921 agtgcgccgc agccgcccga agtcgttccc gaaagcctgc ccgcgcccac cgtcgcccgg
     1981 gccgataagc cttcaacgcc accgcctttc cgagccgagg cgcacgagat caaggaccgg
     2041 ctgcgcgaga tggcgcccat cgaccgcacc gcgccaatcg acgatctctg gcagcgcgtg
     2101 cgctacggct tcgccatgcc cgacctcgac aacgctctgg tgcgaacgca gacgcgctat
     2161 tacgcgacgc gcccggacta tctgcaacgc atcttcgacc ggggacgcct gtacctgtac
     2221 cacatcgtcg aagagatcga gcggcgtggc ctgccgaccg aactcgccct attgccgatg
     2281 gtggaaagcg ccttcaaccc catggcgtac tcgcgcgcgc atgcttcggg cctgtggcaa
     2341 ttcataccgg gcaccggcaa gcgcttcgag ctggagcaga actggtggta cgacgcgcgc
     2401 cgtgacatcg tcgactcgac caacgcggcg ctggactacc tggagaagct ctacgagatg
     2461 catggcgact ggcagcttgc gctcgcctcc tacaactggg gcgagaacgc ggtggcacgc
     2521 gccatcgcga agaaccgggc cgcgggcaag ccgaccgact acgagagcct cgccatgccg
     2581 tcggagacgc gaaattacct gccgaagctg caggcgctga agaacatcat cgccaacccc
     2641 gagcccttcg gcatcgacat cggcccaatc ccgaaccagc cgtacttcgc caccgtgacc
     2701 aagctgcgcg acatcgacgt gcagctcgcc gctcgcctcg ccgagatgcc cgtggaagag
     2761 ttcatcgccc tgaacccggg cttcagccgg ccggtgatcc gcgcatcagt gacgccgcgc
     2821 atcgtgctgc ccgccgacaa ggtcgatgtg ttccacgaca acctcgagcg gctgggcgag
     2881 aagtcgctcg tgtcctggaa aacctactat ccgaagtccg aagaaacctt cgattccatc
     2941 gcgaaaaagc acgccatgag cgtcgcgcag ctgaaggagg tgaacggcat cgcggcgcgg
     3001 cagaagcagg tgcctcggct tctcgtggtt ccaaccaagg atgcgacagt gcaaaagctg
     3061 ccgatcatgt acgcgccgcc gattccagtg acgatgcggc gcgttttcca tacggttcaa
     3121 cccggcgaaa cgctggcgtc gatcgcgaag aagcaccgcg tttccgagga ggacttgaag
     3181 cgctggaatg gtgtgagcaa agccgttccg ggccgcaagc tcgcgctcga agtgcgcgct
     3241 acgccgaaag ccaagccccg cgcgaaagcc aagaccaaag cttcgacgcg gacctaggcg
     3301 tagcccgctt ccaccagtct gcgggtgtac tccgacttcg agcgctcgac cacctgctcg
     3361 agcgtgccgc tttccacgac cttgctgtct ttcatcacca ttacgcggtg cgccatggcg
     3421 cgcacgacgt cgatgtcgtg ggtgacgagg atgtagctca ggccgtattt cttctgcagc
     3481 gacgccagga gctcgaggac ctgcttctgg atcgtgacgt cgagcgcgct tgttggctcg
     3541 tccaggacca cgaccttggg cctgacgatc agcgcgcgcg cgattgcaat gcgctgacgt
     3601 tggccgccgg agaactcgtg cggatagcgc gcgaggagtg gctcggcgcg gccgccttcg
     3661 gtcagcccga cctcgtagag cgcctgcacg acgcgctcgc gccgcgcgtt ggcatcgagc
     3721 gcgggctcgt ggatctccag cccctcgccg atgatctgct ccaccgtacg ccgcggcgaa
     3781 agcgacgaca gcgggtcctg gaacacgacc tggatctctt tgcgcatgtt gcgccgctcg
     3841 gcgcgcgctg cccacgtcct cccgccgatg cgaatgtcgc cttcggcttg catgagaccg
     3901 agcacggcga gcgcgagcgt cgtcttgccg gagcccgatt cgccgatcac gccgagcgtc
     3961 tcgccgggcg cgaggtcgaa gtccaccgaa tcgaccgcgg tgaagcgccc gctcttgaac
     4021 cagccgcgga tacccggcag ccgcgtcgga tagtgcacct gcaccttctt cgcttgcacc
     4081 acctgcccgg ccgccggcgc ggcgacgtcg cgcgccggac ggctgttcac gagcttctgc
     4141 gtgtagggat gctgcgggcg gccgatcacc tgcgcggtcg tgccctgctc gaccagcacg
     4201 cctttctcca tgaccgcgac ccggtcggcg aagcggcgta cgaggttgag gtcgtgcgta
     4261 atgaggagca gcgccatcct cgcctcggcg cgcagccgct cgagaagctc gagaatctgc
     4321 agccggatcg tcacgtccag tgcggtggta ggctcgtcgg cgatgagcag ctttgggttg
     4381 caagcgagcg ccatggcgat catggcacgc tggcgctgcc cgcccgagag ctgatgggga
     4441 tagctgagcg ctcgccgctc cggctcatcg atccccacgc gccgcatcgc ctccaccgcc
     4501 tccttccacg ccgccgtgcc gcgcagcccc gtatggagct cgagcgactc cacgatctgg
     4561 tcgcccaccg tgtagatcgg attcagggcg gtcatcggct cctggaaaat caccgcgatg
     4621 tcgcgcccgc gcagctggtg caattcgtcc ggcggcatgg cgagcacgct tcgcccgtcg
     4681 aagaggatct cgcccgtcag ccgggcgttc tcgatgaggc gcagcgtgga aagcgcggtc
     4741 accgtctttc cggacgccga ctcgcctacc agcgcgagct tttcgccggc gtcgagggtg
     4801 aaggaaacct ggtcgaccac tttgcgggca ctgaagtgaa cgctgaggct gcggacctcc
     4861 agcaagctca tctgcgcagc ccggattcgt cgccggcaag cactttgcgc ggatccagcg
     4921 cgtcgcgcag cgcatcgccg atcaagacta ggagcatcag catcacgacc agcacgccga
     4981 aggttgtcac tgcgatccac caggcatcga ggttttcctt cgcctggttc agcagctcgc
     5041 cgaggctcgg catcccgggc ggcacgccca gcccgaggaa atccaacgcg gtcagtgtgc
     5101 cgattgctgc cgtcatctca aacggcagca gcgtgactac tggcgtcagg ctgttcggca
     5161 gaatgtgccg ccagatgatc ttgcggttgc tctgccctag cgcgcgcgcc gcgcgcacgt
     5221 agtcgagagt tcgattgcgc aggaattccg cccggatgta gttagccagc cccagccagc
     5281 cgaacatcga tagaaggacg actagcagcc cgaagctcgg atagaagatg gcggaaaaga
     5341 taatcagcat atagagctgc ggcagggagc cccagatttc ggtaaagcgc tccatgccga
     5401 tgtcggtcca gccggcgaag tagccctgga tcgcaccata gagaacgccg aggatcgtgc
     5461 aaagcccggc gaccaggatc gcgaatacga gcgacacgcg aaagccgtag agcagccgcg
     5521 cgagtagatc gcgcccctta tcgtcggtgc cgagcaggtt ctcggcactc ggcggtgccg
     5581 gtgccggcgc ttttgcgaaa tagttgaccg ttttgtagtg atgccgattg atcggaaaaa
     5641 cggcgaagtt gccgggcgca gcgaggttcg ccttgatctg cgggtcgtga aagtcggtgc
     5701 gggtcggaaa ctggccgccg aacgtcgtct cagggagatc ctggaacact gggaaatacc
     5761 acgcgccctg atagcgcacg gcgagggggc ggtcgttgga caccacttcc gcgagcaggc
     5821 tgattaggaa cagcacgcca agcacgatga ggctggcaag gccgaggcgg ttgctgcgaa
     5881 aacgtagcca ggcgcgcagc gtcgggctga catggactgc atcctgcgtc gccgacgtgc
     5941 tgccgagcga caggccgcta gcgacggtct cggtcggcgt actcaatcga agaacttcac
     6001 gcgagggtcg acccagacgt agcttaggtc gcggatcagc gtagtcagca agccgatgat
     6061 ggtgaagatg tacaggctac cgaacactac gggatagtca cggcggatca ctgactcgta
     6121 ggacagcaga ccaagcccat caagcgagaa cagggtctcg atcagcagtg agccgccgaa
     6181 gaaggcgccg atgaacgccg cgggaaaccc ggtcatgatc gggatcaggg cgttgcggaa
     6241 cacatggcgc caaagaatgc ggtgctgagc cacgcctttc gcgcgcgcgg tcatcacgta
     6301 ctgcttgcga atctcctcca acatcgagtt cttggtcaga atggtgatca ccgcgaagcc
     6361 gcccaccacc atcgcggtca ccggaagcgt gatgtgccag aggtagtcgg tgatctttcc
     6421 ccagagcgaa agttggtccc agttgagcga gacgatgccg cgcagcggaa accactgcac
     6481 gaaggtgccg cccccgaagt acaccagcag cacgacccca agcacgaagc tcggtatcga
     6541 atagccgagg aggataagga acgtggtcgc ggtatcaaaa ggcgtgccgg cgcgcaccgc
     6601 cttggcgatg cctagcggca cggagattag atagatgagc acgaaggtcc acatgccgag
     6661 gctgatagag accggcagct tctccttgat cagctcccag acgctcttgt tatggatgaa
     6721 gctgcggcca aggtcgaagc gtgcgaactg gccgagcatc tggaagaagc gctcgtgcgc
     6781 cggcttgtcg aacccgtaga gcttcctgat ctcctcgatc tgcttgggat cgagaccttg
     6841 ccggccgcgg tagccgctgt ccccgctcgg cgcgccgacc tcgcccgcgc cgaggttgcg
     6901 gccctcgagc tgggccacca tctgctccac cgggccgccc ggaacgaact ggacgatgac
     6961 gaacgtgatc acgaggattc cgagcagcgt cggaaccatg agcagcagcc gcctcaggat
     7021 gtaggaccac atgcgctatt tttttcgcca ccaggttctc agcacccagt cttccgcacc
     7081 gtagtactgc ggagcaacgg ccggctgctc gaacctgccg gcgcgatagg acacgcggaa
     7141 gttcgccgcg taccaatgag ggatcacgta gtggctgtgc cgcaggaccc ggtccagcgc
     7201 ccgcaggctc gcgaccagat cggggcgcgt ggtcgaggaa accaccttct ccagcagcgc
     7261 gtctacgccc ggatccttca cgccaatcag attgctcgaa cccggcgtgt cggcagagtg
     7321 cgagccaaag cgactgagca gctccgcgcc cggcgcctcc tgccccggca cgcgcatcga
     7381 gaacagatcg aagtcgaaga cctgcagccg cttctggatc agcgcgaaat ccgcccgccg
     7441 gtatcgccct tcgattccca gcctctcgag tgcctgcgca tacggaacga agactcgctc
     7501 aaacgaacct ccgtcgaggt attcgacgac cagagcctcg tccttcgcgt tgcgcagcgc
     7561 gccgtcgcgg tacgtccacc cggcctcctc gaggagcgcc ttggctttca acagattggc
     7621 gcgcaagctt cccgggggat tcgtcgatgg cggtagcggc acgtcctggc tgaagacctt
     7681 tgcaggcagc gtcttgcgca gcggctcgag cagcttgagc tcgtccgccc cgggcagtcc
     7741 cttcgcctcg aaatcgctgt tattgaagaa gccgcggacc cgcgtatagg agttgtacat
     7801 catctgccgg ttctgccact cgaaatcaaa ggcgagcgac agcgcctccc gcaccctcgg
     7861 atccttgaac ttctcgcgcc gcgtattgat gatgaaaccc tggaagtcgc cggcgttctt
     7921 cgagcgccat tcgccgcgga tgagctcgcc cgagtcgaac ttcttgccgg tgtagcgtcg
     7981 cgcccaatcg cgcgccgaga acacctggat gtaatcgaac tccccggccc ggaacgcttc
     8041 cgtctgggcg gtgttgtcct tgtagatctt gtaggtaatg cggtcgaagt tgaacatgcc
     8101 gcggcgcacg ttcaggtcgc gcgcccagta gttcggatcg cgctcatagg tgatgtcgcg
     8161 gccgaagttc atgcggccaa tcttgtaggg gccgctggcg atgggatgct cgctgatcac
     8221 ctcgtcgaac tgcttgccgg cgccccaggc cctgctgaaa acgggaagcc ctcccaccat
     8281 taagggcagc tccggacccg cattccgaaa gtcgaagcgc acgctgagcg ggccggtgac
     8341 gaccgcttgc ttcacgtctg ccaacgcgaa gcggtaagcg ggcgacgcct gcttgctcat
     8401 cagggtgtcg aaggaatgct tgacgtcggc ggcgctcacc ggcgttccgt cttggaaacg
     8461 agccttcgcg ttcaacctga aggtgaccga caggccgtcg ggcgccagcg ccacgtcttc
     8521 ggccagcaag ccataagcgg ttgtcggctc gtccaacgtg ccggtcagca aagactcgaa
     8581 caccagcgcg cccagacccg ggggcgagct tccccggatg gtgaacgggt tgaacttgtc
     8641 gaagttcgtg atgcgcagcg gcggcaccag ttcgatctcg ccgcctctcg gcgcgttggg
     8701 gttcacccat tcgaaatggg tgaagccagg cgggtacttg atgtcgccga actgcgagta
     8761 cgcgtgcgcg gcccacacgg gctttgcaca aaggaacacc actgcggcaa ggaaaacctt
     8821 catgccttct cagtcgatcg tgggaacggc cgcgaggagc tgcttcgtgt acgggtgctg
     8881 cggccggcgc agaacatcgt cgacggcgcc gcactcgacg attttcccct ggtacatcac
     8941 cgcgacctcg tgcgccaggt agtccacgac cgcgatgtta tgcgtgatga agagatatgc
     9001 gataccgagg cgctgctgca gcgcgcgcag caggttcaag atttgtgcct gcaccgagac
     9061 gtcgagcgcg ctcgtcggct cgtcgcacac gatgagcttc gggttcacgg cgagcgcgcg
     9121 ggcaatcgcg atgcgctggc gctgcccgcc cgaaaactcg tgcggatagc gctcgagcgc
     9181 gttcgcgggc aagccgacgt cggccagcag gttttgcaga cttcgggtcc tctccaaatc
     9241 gttcgcgcca atgcccaggc tgcgcatccc ctccagcagg atctcggcca cgcgcagccg
     9301 cgggttgagc gacccatagg gatcctggaa gatgatctgc atcgccccgc gccgcgggcg
     9361 caggtcgcgc cgtgagagtc cggtcagctc gacatccttg aaacggacac tgccgccggt
     9421 cggcgcaatg agctggagaa tcgccttccc ggccgtggtc ttgccgctcc ccgactcgcc
     9481 gaccagcgcg agcgtgcgcc cttcgcgcaa ctcgagcgac acgccgtcca ccgcccgcac
     9541 atggcccacg gtgcgcctga agatcccctt tctaatgggg aagtgcacct tcagatcttt
     9601 cgcttgtaaa agaattccac tacttgcctc gctccgcacg gtcccctccc ctgcgctgtc
     9661 gctagtgctt ctagggggag caagactctc ctcgcgcaaa tggcaacgcg caaaatggtg
     9721 tggcgccggt tccagaagcc ggggcgcctc cacgcggcag cgctcgaaca cgtagtcgca
     9781 gcgatcggcg aagcggcaat gatcgaattt cttcgtgagc ggcggaacct gaccgcggat
     9841 caccgcaagg tcgccggcgc ggcgctgcgg cgtcggcagc gcggcgaaca gcttctgcga
     9901 gtaagggtgc tgcggcgcgc gcaggaagag gtctcgctcc gcgagctcga cgatttcgcc
     9961 ggcgtacatc acggcgatgc gctgcgccat cctggccacg atgccgaggt cgtgggtgat
    10021 gaggaggagc gccatgccgc gctcggcctg cagcctcgcc agcaggtcca ggatctgcgc
    10081 ctggatggtg acgtcgagcg cggtggtcgg ctcgtcggcg attagcacct ctgggtcgac
    10141 ggcgagcgcc gcggcaatca tcgcgcgctg ctttaggccg ccggagagct ggaacgggta
    10201 ttcgtcgaag cgccgctcgg cgtcctggat tcctaccgaa cggagcagtt cgagggcgtt
    10261 atcccgggcg gccaaactcg aactactctt atgcctctcg atgacttcca caatctggag
    10321 gccaacggtc agcacggggt taagcgcggt cgagggctcc tggaacacca tcgcgacgcg
    10381 ccggccgcgc acggcgcgca tcgccgcctc ggggagcccg agcaggtcga tgccgtcgag
    10441 cccgacgcgg ccggaggtga tccggccggc ttccggcagg aggcgcatga ccgaaagcgc
    10501 ggtcatcgac ttgccggagc ccgattcgcc gaccagcgcg aagcactcgc ccttgcgcag
    10561 ttcgaaatcg ataccgtcga cggcgcgcac cgctccccca gcggtgtcga gcacggtccg
    10621 gagattctga actgccagcg tcatacggcc agcgccccca cccggacccg cggatcgaag
    10681 ccgtcgcgca ccgcgtcggc gaggaggttc gctgcgagca ccagcacgaa catgaacacg
    10741 aacgctgcgg cgagcgacca ccagacgatc ggctcgcgcc ccatctcgag ccgcgctgag
    10801 ttgatcatgg tgccgaagct cgtcgtcgcg ggatcgacgc ccacgccgac gtacgagagc
    10861 accgcctcgg cgagcacgag gccggaaaag tccatcacca tcgcgatgat cacgatgtgc
    10921 gagacgttgg gcaggatgtg ccgccccagg atgcgggcgt cggagacgcc gaaggcgtgc
    10981 gcggcctgga tgtattcgag ctgcgacagt ttgagcgcct cgccgcgcaa caggcgcgca
    11041 agcccggtcc agctcgtgag ccccaggatg gcgcacagca cgagcaaccg gaagtcggcg
    11101 cgctgcgccg ccgtcggaaa ccattcggcg tggcggtcga tgacgacctg catcatgaga
    11161 accgccgcgg cgatgagcag cacgccggga atggagttga gcgtcgtata gagatactgg
    11221 atggcgtcgt cgatccagcc cttgaagtac cccgccatga tgccgagcgc gatgccgagc
    11281 ggcaggagta cgagcgtggt cagcgtgccg atggcgaggc tggtgcggat cgatttcagg
    11341 gccaggtaca gcacgtcctg tcccacctgg ttggtgccga gcacgtggta atgcggcgca
    11401 aggctcgcga tcggcccggc cagcccgagg atgacggcaa gagtgatcca caccgcgccg
    11461 aacggaacgt gcggatagcg gcgcgccagg ggacgcgaga taaagaagag cagcgcccac
    11521 aataccgccg cggcggcgag gccggcggcg gcacgcaggc ccacatcgct ccagcgatcg
    11581 agttcctgtt gcaaatgcga gcctccgtgg cgaagccgcg gaaaatcgcg tacctgcttg
    11641 ccgtccggcg tctcgatctg ttccttggag taaagccgcg tcgcgagcgg cgcggaatag
    11701 gtcttctcgc tcttttgccg cagcatcgcc agcgtgcggt cgagcagcga tagcacctcg
    11761 accgacccgg cggcgcggta gtggagcgag tcggcgagcc cgaccaacag gtagcagccg
    11821 agcaccacgc cggaaacgac cgcggctttc gactggaaca cccgcttcca cggcgcggcg
    11881 aggtgcggcc ggcggcggca gtacaggccg taaacgacga ccgccgcgac cagcagccag
    11941 aggagcgcat cggtggcgag gatgaccggc gtcattccag cctgatgcgc gggtcgacca
    12001 ccgtgtacga aaggtcggtg agcagcagcc cgacgatgta gagcacggag ccgatgaaca
    12061 ccatcgagcg caccacggcg aagtcctgcg cctggatcgc atcgatggtg tagctgccaa
    12121 gccccgggat gccgaagaac gactcggcga gcaggctgcc catgaaaagg agcgggatga
    12181 cgacgaccgc gccggtcagg atcgggatca tcgcgttctt gaggacgtgc cggaacagca
    12241 ccacggactc ggcgagcccc ttggcgcgcg cggtgcgcac atagtcgcgc cccatctctt
    12301 cgaggaagat ggtgcgatac cagcggctgc ccgagccgat gccgctcacc acgccgatca
    12361 gcaccggcag gatgacgaac ttcgcggcgt cgaagccgcc gtcgtagccc gagatcggca
    12421 cgaggtgcag gatcttcgag gccatgtact ggccaccgat gatgtagaac agcgtcgaga
    12481 tcgacatcat cgccacgcac agcaccacgc cccagaagtc gaggtaggtg gcgcggaaaa
    12541 acgccatcag tagcgcgaac gtgatgttga ccgcgagccc cacgaggaac acgggaatgg
    12601 cgagcgcgag gctcggcccc atgcgcgtgc ggatctcgtg cgcgatgtcg cgccggtcct
    12661 cggcgcggcc gaaatcgaag gcgaagagct tcaccgattt gtcgaaaaag atggtgtcgg
    12721 tgaccttgcc gagcccgggc gctttttcgt tccacagcag gtcgcggtcg tatccccgct
    12781 cctgcttcca cttctcgatc gcctccggcg ttacgcgctt cgcgcccagg tgcatgcgtg
    12841 ccatgtcatc gggcgtgttc accacgaaga agagcacgaa agtgatcagg ttcaccccga
    12901 tcaggatcgg gatggcgtac agcacgcgcc ggacgatgta cgccagcatc aggccgcggg
    12961 tcgtgcggcc atgcgctcgc gttttctgta ggtgcgaacc gcgggaacga tggaaattgc
    13021 cagcgccaga agcagaagca agacaggcca gacgaccggg cgattccact ggcgcctgaa
    13081 ttcggcacgt cgctgcgggt cgacgcgcag gtactgcatc gtgttgcccg ccatgtcgtt
    13141 cggcttcacg ttggccagcc actgatggcg cagtgtgtaa tccttggggt ggaagccgaa
    13201 gatccacggc gcatcgcggc gcgcgatctc caccatacga tcgatgatct gctgccgctc
    13261 cggcgagttc gacatgctct tcatgcgctc gaagagcgca tcgaactccg cgctcgtgta
    13321 gttcgagctg ttcgagccag cgccgtcggc gcgcgcttgc ggcccatgca gcagaaagag
    13381 gaagttctcc gggtcgggat aatcggcgtg ccagcccagg ccgtagacct gctgcgtgcc
    13441 cttgcgtatt ttttcctgaa accggttcca gtcggtctcg cgcacttcga gctggatcga
    13501 gagcttttcg aactgcttgc gccaccagtc gaggcgcgcc ttgtcgcccg gtccgcgcga
    13561 ggtcgtgtcg aagtaaagca cgagcggcgc gccggtcttg gcgtccgtgc cttccgggta
    13621 gcccgcctct gccagcagct tcttcgcctc caaaagcgcg cggcgctgct cgcggccatc
    13681 gcgccacaca tgcgtgatcg gattcatcgc ctcgcgccct tcgcgcgcgc cgaagatgcc
    13741 cggcggcacc gggctgtgcg cgacgatgcc gcggccgttc aggaaaatcg acaggtactc
    13801 ctccatgtcg accgcgatcg agatcgcgtg gcgcagcttg cgcgcgcgtt cggtgttccc
    13861 gcccaccacc gggtccttca tgttgaagcc gaaatagaac accgtcgccg cgacggcggt
    13921 gctcagctcg atgccgcgct cgagcatctc gggcgaaggc tcggcgccac cgtcgaccgc
    13981 gacgcgcacc gcgtggtcga agttctcggg gctgatgccg gcactgtcgt agtagccctg
    14041 caggaacttg ttccagtaag ggacgctttc cttctcccgg ctgaagatta cgcgatcgat
    14101 aaagggcatg gttttccccg catcttttag caagcccgcc tcgccatcgc tcgattcgcc
    14161 ttccgcggga tatggcatgc cgcgatagtt cggattgcgc tcgagcacca tgcgcgcgtt
    14221 cggatcgttc tcccgcagca tgtacggccc ggtgccgacg ggccaccagt cgagcgtgaa
    14281 attcttgagc tccatgccgg gctggtggaa caacttttcc gcttcccatg gaatcggcga
    14341 aaagaacggc atcgcgagcc agtacacgaa ctgcggatag cggcccttca gcgtgacgcg
    14401 ataggtgtag cggtccactt cctcgacgcc ggcgagcgga tgctcgcgca agtcgatcca
    14461 gccgggccga ttgagcttct tcagcttatc cgcgaattcc ggcagcccga cgacgtattc
    14521 cgccatcagc ccgtagatcg gcgagtgcag ccgcgggtgc gcgagccttt tgatctggta
    14581 gacgtagtcc gcggcgacga gctctcgcgt gccgagcgga aggtcgtacg gggttttcag
    14641 cttcttgatc tcgctcgcgc tcagcgcctg attgccttcc acaaacgcag gatgaggctg
    14701 gtagcggatc ccgggccgga tgcggatctc gtagacggtg aacttgccgc catcgatctc
    14761 cttcggtttc ggcacctcgg cagccgtcag cgggatgagc tcgtaagggc gcttcaggta
    14821 gtggtagtgc agcggcggct cgtagacctg acgcgtaaaa agcgactcgt ccactgtata
    14881 ggactgcacc gggtcgagat gcttcggccg attgacaaac gccgagtaca ggatgttggc
    14941 gccgcgctcc gagcgcgggt aaggatcgtt ccagaccgcg ccgcagcctg ccacaaatag
    15001 catgagcatc aaaaggatgc gcacgccgct cagtataatc ggccgatggc gcttctttcc
    15061 ggcaagaaga tcctcgtcac cggcgtgctc tcgaaccgat cgatcgccta cggcatcgcc
    15121 aaggccgcgc gacgcgaagg cgccgaaatc gcgctcacct accagaacga gcgcttcaag
    15181 gaccgcgtca ccgacatggc gaaggagttc ggctcgagcc tcgccctgcc ctgcgaagtg
    15241 acgagcgatg ccgaaatcga gtcgcttttt ggcggaatca ggaagaactg gggcgggctc
    15301 gacggcctcg tgcacgcgat cgctttcgca ccccgcgagg ccatcgccgg cgacttcctc
    15361 gatggcgtaa gccgcgaggc gttccgtcag gcgcacgata tctcggtcta cagcttcccg
    15421 gcgctcgcca aggccgccct gccgcttcta agcaagaacg cctcgctttt gacgctcacc
    15481 tacctcggcg ccgcgcgcgt ggtgccgaac tacaacacga tggggctggc caaagcgagc
    15541 ctggaagctt cggtgcgcta tctcgccgca agcctcggcc cacgcggaat ccgcgtgaac
    15601 ggcatttccg ccgggccaat caagaccctc gccgccgccg gcatcgccgg gttcggcaag
    15661 atcctcaagt tcgtcgaaga gcacgcgccg ctgcggcgca acgtgaccac cgaagacgtg
    15721 ggcaacgccg ccgccttcct cttatccgat ctggcggccg cggtgaccgg cgagattctc
    15781 tacgttgacg cgggcttcag ccacgtcgtc gctggaatcc ccgcaaccag cacggaggca
    15841 taggactccg gccctttatt tggcttcgag gttcttctgg ttgatctcga cgtcggcttg
    15901 ggcgcgcagg ctcgcgatgt aggcttcgta ctgcgcgccg ccgaagagct gcgccgcgcg
    15961 cgcgctgacc tgcggatcgc cctccttgac cggctcgtcg atgaccttgg agatccggat
    16021 gagcgcgtag cccgcgtccg ggagcggaac gccgatgtag acgggaagct tggcggtgtc
    16081 ggccgacatc acctggcgca gcatgtttcc gctcagcccc tgcgcttcgc ggcgtgacac
    16141 gacccgcggc gcgctccact tgagccccgc gtcctgtccc ttgcgcagct gctcgagctt
    16201 cgccgtgcct tccttcagcg cgagcgccga ggcctcgcgc cggcgcagct gctcggcgat
    16261 ctcgcccttc atctcctcga agctgcgctg cgccgccggc tggtggtcgg tgatgcgcgc
    16321 cgcgaccagg gtgttcggtg ccacctcgat cgcatcggtg ttgcggcggt tcttgatcgc
    16381 gtcggaagtg aagagcgccg cgagcagctt cggattgtcg agcgcgccga gctcctggcg
    16441 cgccgacttg ctgatccagc cggtctgttg caccggcagc ttgaagcgct cggcggccgg
    16501 cttcagcgta tcggactgct cgtacaccaa gttcgagaaa gtctcggcgg cctcggcgaa
    16561 cctgcgttgt cctttgaccc gcgcaagctc gtcggtaagc tccttcttca cttcctcgta
    16621 cggccgcgtc ttgccgggct gcacgccggt gagccgaatc acgtggtagc cgaactcgct
    16681 ctcgaccacc tgcatctcgt tctggccaag cttgaacacc gcctcctcga acggcttaac
    16741 catcatgccc cgcccgaacc agccgaggtc gccgcccttc tcggcggagc ccgggtcctg
    16801 cgaatgcttc ttcgctagtt cggcgaagcg cgctggggct tttttcgctt cctgcaggat
    16861 cttctcggcc tcatccttgg ttttcaccag gatgtggctc gcgcggcgct gctcctcgac
    16921 gcggaactgg ttggcgcgcg agtcgtagga agccttgatc tccgcctcgg tcggcgggtc
    16981 ctggcgcgaa agcgcctcgg ccgagagcac gacgtattcc gcgcgcactc gctcgggcgt
    17041 gcggtagtcc gcctggttgg cgtcgtaatg cgccttgacc tgcgcctcgt cgatcttcac
    17101 ctgcggcaga aactgcttct cggaaatgcg cacttcggcg atctcgcgct tctgcgcttc
    17161 gagcgcggcg aggcgggcgg cgacgctgcg cggggcgatc gccgtctcgg agatcgcccc
    17221 ggcgacgcgc gcgagcggga tctggctttg cagctcggcc acgttgccct ggtccgagat
    17281 gccgcggctc gaaaggtagg ccgaatactt ggccgtggag aacttcccgt cttcctggaa
    17341 ctccggcgcg gaggtgatcg cgtcgatcac cgcctcgcgg ctcatgatca tgtggccgcg
    17401 cgccgcctcg cgcgcgagca ggcgctgaga gatcagtcct tcgagcaccg caacgcgcga
    17461 ttccggagta tcgaggagcg cggggtcgat ggcgccgccg aacatgcggc gcatctgctc
    17521 ctgctggcgg cgcagctcct cggcgaactc gcgccgcgag atctccatgc cgtcgacgct
    17581 cgccactgtg tcgcgcgagg agcggaactg ggtgtacgac tcgatgcccc aggtggcgaa
    17641 ggtgatggcg atgagcgcga ggaaaacctg gatgacgcgc ttgttcttgg taacgaggtc
    17701 gaacacgcgc gcattttacc ctctcagtaa ccgcgctaca gcccgcgaac cgctcgccgc
    17761 cgcgactttg gtaaattgac gctaaccaag gagaccagaa tgacccgctt tctgcccctg
    17821 ccgttcttga ttgctctctt cgtcgcttgc gcgcaggcgc gcgacgtccc ctacgtgccg
    17881 acgcccgaga aagtcgtcga gcgcatgctc gaagtggcga aagtcgggcc gaacgacgtg
    17941 gtctacgacc tcggctcggg cgacgggcgt atcgtcatcg cagccgcgaa gaaccacggc
    18001 gcgcgcggcg tcggcattga catcgatccg gagcgcgttc gcgaagcgcg cgacaacgca
    18061 agccgcgcgg gcgtcgccaa ccgcgtcgag ttccgcgagg gcaacctctt cgacgccaac
    18121 atcagcgagg cgacggtcgt gacgatctac ctgctctcag gcatcaacat gaagctgcgg
    18181 ccgaagctcc tcgccgagct caagcccggc acgcggatcg tctcgcatgc ctttgacatg
    18241 ggcgattgga agcccgaggc caccgccaag gtgggcacga gcacggtgta ctactgggtg
    18301 gtaccgccgc gaaaatagaa aaggggctgc gtgcgcagcc cctccccgtg cggccctctc
    18361 aatccagaat gtgcgggtag atgaagccga acgaaatcat gcagaccgcg accttgagga
    18421 cgaacttgac cttctggagc ggtcgcatct cgtcgaactt gagggttcgc tgagccgtcg
    18481 ccgcactccc ctgcgcagct tgcttcgggt tggacatggt ttctccttga cgaagcgatc
    18541 cgtgggcgcc ggcaagatgc cgcgggagta ggccgccgcg cgaatcgcga gccgattcta
    18601 gccgccgtgc cgcaactctg cgtatttctt cctaacagcc cgggcgcccg gaggaagata
    18661 ttcgcggtgt atggcggcca aacagctaaa gaccgtcctc ttcgccgaag tcagcggcag
    18721 ggatgagtat gtgcgcgccg ccgggcgcgt gatcggctgg aatggcattt ccgactgcgt
    18781 aaacagcctg cagcaaaccg cgcgctcttc cggcggcaaa gtcgtgaagg tcatcggccc
    18841 cgagctgatg atgctgttcg aaagcccgga tgcggccgca aacgccgctt ccaggatgca
    18901 cgccgccgtg aactcgctgc ccacggttgg aggagccaag ctgtccgtca aggtcggcta
    18961 ccacgccggt cccgttatcg agggcagcga cgacctactc ggcgatacgg tgcagcaggt
    19021 ggcggcgctc ctgcgccagg cgcacgcggg agagaccatc actacgcccc agactgcgcg
    19081 actcctcaat ccgtcctttt cgggacaagt tacggtacct ggctcggccg gcggtattca
    19141 ccgtacgcgc gcgcagcgca tgctgcgcct cacctatgga agcgaatccg cgacatgcgg
    19201 gcccgacaac cgcatcgtcc tcgtcggccg agaccctgct tgcgcattgg tgacacccac
    19261 cgccttcgca tcgcgccggc actgcacaat cgtcctcgcg gcggacggac tcaaggtgcg
    19321 cgatcacagc agcctgggca ccttcctcac caatgaaggc agcgaggaga tccagctcgc
    19381 taacggacag gaagtcgcgc tcggcacgca cggctggatt tcggtcggct gcgcgcgcgc
    19441 gttcgcttcg cgactgctcg agttcactgg tcagtagcgc tggtcggccg ttatcgcagt
    19501 cgcgttcttt caatcaggcc gcggcttacg gaacggtcgg ccggcccaag gccgggcctt
    19561 tgcgccatag gcccgcttgg cggcgagcaa ccgcgccagc gtgaactctt cgcgggaccg
    19621 cagtccgcgc actacgttcg caacccgccg cggcgacatg ccggtatcga tgaagtgccg
    19681 cggcaggtcg ggtccccagg cgtagaggcc tgacacgccg ccctggtggt agcgagtcgg
    19741 cgcatcggca tcgaacacgt cgccgtccgc gtaatgctcg acggcgaagc cgctcggatc
    19801 gtagtggtag cagaagatct gcgagccgaa atcgcgttat ccgtgcggac ggacaagcgc
    19861 aggccgaaat cgaggaagta gcgttcggcg cgcccgagat cccgccgggc gaagcgcacg
    19921 tacgctacgt cggcgacctt tatgaccggc gctgcggccc gcgggatgaa tacagtcaaa
    19981 ttctcaaagc aagacgggcg gtaactaccc cggtgttcag cccgatcgtg cgtaggccgc
    20041 cgaaaaaccg cgcgtgctcg atcttgcagc agcggtccat gacgacctgc acgcctgcgt
    20101 cacgcgcgat ctgggccgct tcctcgctga tcacgccgag ctgcagccaa agaaccttgg
    20161 cgccaatctg gaccgcttga cgggcgatcg cgggtgcttc gcgcgccggc cggaagacgt
    20221 ccacgatttc gatcggccgc ggatgcgcgg ccgcggccgc ggcaagatcc gcatatacag
    20281 gtcggttcaa gatcgcgcgc ttgcccgctt gcgtcgggtt cacgggaacg atgtcgtatc
    20341 cgtttgcgtc gaggtagatc gccgcgaacg agcttgcgtt gtagtagttc gcgctcagtc
    20401 cgaccatcgc gatcgtgcgg tactgggtca ggatgcggta acgttcctgc ggggcaagtc
    20461 cgaagttgtt gcgctcgccg acggcgctca tgactggtat ggcgccccgt aagccttgga
    20521 gagaattgag tgaccgacac cctgttcgac ctttgcgcgc ccggcggggg ggccggcctt
    20581 ttgcgacgcc gccagcgcct ggtccaggtc cgagacgata tcggcaacat cctcgatgcc
    20641 aatcgacacg cggaccagct cgggcccgat gcgcgcggca cgcagctcgt cgtcactgag
    20701 ctgctggtgc gtggtcgaag ccggatgaat gaccagcgac ttcgcgtcgc cgacgttggc
    20761 gaggtggcta aaaagctgca ccgattcgat gaacttctgc cccgcctccc ggccgccctt
    20821 gatcccgaac gtgaagatgg agcccgcgcc cttgggcagg tatttcctgg cgagcgcgtg
    20881 atattgattg gacgcgaggc cgggatagtt gacgtactcg accgcccggt ggctctcgag
    20941 gaactcggct accttctgcg cgttctcgac gtggcgcgcc atgcgcacgt gcagcgtctc
    21001 caggccctgc agcatcagcc aggcggtgaa cggcgccatg gtcggaccat agtcgcgcag
    21061 catctcggcc cggcacttca gaagccagct gaagtcgccg aaataatcga agaagcgcaa
    21121 atcgtggtag cccttcgagg gctccgtcat tcccggaaac ttgccgttgt cccatgggaa
    21181 gcggccaccg tccacgatca cgccgccgat tgccgtgcca tgcccacaga tgaacttggt
    21241 cgccgagtgc agcacgatgt cggcccccca ttcgagcggc cggcacagat acggcgaggc
    21301 gaacgtattg tcgatgatga gcgggacgcc gacctcgtgc gccagcgccg cgagcggctc
    21361 gatatccacg atgtcgatgg tcgggttgcc catgacctcg gcgtagaacg ccttggtctt
    21421 cggcgtcacc gccttcttcc actgctcgag gtcgctgccg tccacgaagc gcgcctgaat
    21481 gccaaccttg cggaagctga cgtcgaactg ctggtagcta ccgccgtaaa ggctgcgcga
    21541 cgccacgaat tcgtcgcccg cctgcatcag ggtgaaaagc gccaggtgct gcgcagcctg
    21601 ccctgacgcg gtcgccacag cgccgatgcc gttctcgagc gcggcaatgc gctcctcgaa
    21661 cacggcgacg gtggggttca tgatgcgcga gtagatgttg ccgaagcgct gcagcgcaaa
    21721 gaggttcgcc gcgtgatcgg tatcctcgaa tacgtatgcc ccggtctgat agatcggcac
    21781 ggcacgcgat ccggtcacgg gatccggtcg ttgaccggca tgcacggcga gagtatcgaa
    21841 gccgaactgg ctttcgttcg ggacgcgttc gcgcttcatg gggccttcct cgcttccgcc
    21901 tgagaaatgg gttccaggcc cgtcttggcg atgacgggtg ctgcatcggc tgacgtgaag
    21961 tacttgatca gcgccttggc cgcctctggc tctttcgcgc cgacggcgat gcccgccgag
    22021 aagatcgtga cttgctggac ctcggccggc agcggaccga cgtaatcgat gcccggaatg
    22081 ggcaacaact cgctcacctg ctgaaaaccg agctccgctt cgccgcgcgc aacgatggtg
    22141 ccgacgcgct cgctttcgat tcgcttcgac ttatccttga tcgcatccca gatgccgagg
    22201 cgcgggaaca ggttggtgga cagataaacg ccgctcgcgc ttgccgagta agcgatcgat
    22261 tttgcctcga gcagcgtccg cttcaaggct tcgacgctgc tgatgtccgg ctttggcgcg
    22321 cccgcgcgaa ccgacatccc gatggtcgag cgcacaagat cgacgcggct tcccggcacc
    22381 accttgccct gcttaacgag ttcgtcgagc gctggggcgg caaggatgac gatgtcgacg
    22441 ggctccccgc gcgcgagccg cgacggaatc gagtccggcg cgccgcccat ggacgcgccg
    22501 tacatggtga cgatcttgtc gcccgtcagc ttctcgtatt gcggggtcag cgcgttgtag
    22561 gcagcggtga atccacccga agtcatcacc ctgatctcgg cggcgttgac cagtcccgcc
    22621 cacagcgcga ccaccagcaa cacggcatgt gacgagcacg gcaggattct tcgtgtcctc
    22681 ttcataaacg ccctcgagga atatgaatag tcctgaacag tacagccggg ccgcgccggg
    22741 gacaatacac gcgctaagat gatcggctcg tcgacgccgc cgagcagttc aaccagcagg
    22801 gcatcgagcc gcgcttcgtc atcctgctgc acgcgcaggc cacccagttc gccgcgcgca
    22861 cgctcgccgg gacgaagttc aaggagccgg aaggcgacga ttttcgcgca gcgcaggatc
    22921 tgctcaggcg cttcgccgca ctcggcggac gaaccgaggt gtgcgggatc gccatggacc
    22981 gctgcgcgat cgcgcgagac aacctgatcg aatgcgtcgt ggtcgagcgc aacgtgttcg
    23041 tgaactcggt tgcgctgcag aacaagggct acgcctacat gccgatcttc tgaagcgccc
    23101 ttgagattgg cggagcggac gggactcgaa cccgcgaccc ccggcgtgac aggccggtat
    23161 tctaaccgac tgaactaccg ctccttcctg cagcgaactg gttggtgggt gctgtagggg
    23221 tcgaacctac gacattcgcc ttgtaa
//
LOCUS       DASTPD010000018        24122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_210609, whole genome shotgun sequence.
ACCESSION   DASTPD010000018 DASTPD010000000
VERSION     DASTPD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210609"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2834
                     /gene="rpoB"
                     /locus_tag="VFR57_00725"
     CDS             <1..2834
                     /gene="rpoB"
                     /locus_tag="VFR57_00725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU0257930.1"
                     /translation="NKLEVRVEPRLTLSPKDIVEVIRILVELRNGRGEIDDIDHLGNR
                     RVRSVGELAENQFRAGLVRVERAVKERLSQAESENLMPHDLINAKPISAAIKEFFGSS
                     QLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGP
                     NIGLINSLALYARTNKYGFLETPYRKVKDSKATNEIVYLSAIEEGNYVIAQANASLDK
                     AGKLTDTLVSCRFKNEFELKSPEEVQYMDVAPSQIVSVAASLIPFLEHDDANRALMGS
                     NMQRQAVPCLRPEKPFVGTGVERTAAIDSGTCVIALRGGLVDYVDANRVVVRVNDDET
                     VAGDVGVDIYKLTKYTRSNQNTNINQRPIVKQGEKIAKGDVVADGASTDLGELALGQN
                     MLVAFMPWNGFNFEDSILISERVVADDRFTSIHIEELTVVARDTKLGPEEITRDISNL
                     SEAQLARLDESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKD
                     TSLRVPSGISGTVIDVQVFTREGIERDKRSQAIIEDELKRYKTDLADQMRIVEADTFE
                     RLERLLLNKTANGGPKRLAKGTKVTKEYIDGVEKYDWFDIRLANEESAAQVEGLKDSL
                     AQKRREFDAMFEAKRKKLTQGDELPPGVLKMVKVYVAVKRRLQPGDKMAGRHGNKGVI
                     SKITPVEDMPHMADGTPVDIVLNPLGVPSRMNVGQILETHLGWAAKGLGFKIGALIKQ
                     QQKLAELRKTLEAIYSGRDEDVSQLTDEEVLQLAQNLREGVPFATPVFDGAAESEING
                     MLEMASLPTTGQVQLYDGRTGEAFDRNVTVGYMHMLKLHHLVDDKMHARSTGPYSLVT
                     QQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDITGRTKVYENIVKGDH
                     KIDAGMPESFNVLVKEIRSLGIDIDLERY"
     gene            2834..7054
                     /gene="rpoC"
                     /locus_tag="VFR57_00730"
     CDS             2834..7054
                     /gene="rpoC"
                     /locus_tag="VFR57_00730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU0257931.1"
                     /translation="MKALLELFKQVQQDEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGLTPLKRTQLL
                     TEDDYLAKVEEFGDDFSAAMGAEGIRALLRSLDLNKAIDELRKDLEGTTSDAKIKKIA
                     KRLKVLEGFKSSSIKPDWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLELLGLAT
                     TIKAAKRLVEQEVAEVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDIVL
                     GLYYATRERINAKGEGMSFSDLAEVHRAYDSRQVELNARIWVRIKEKELSDSGEQTEK
                     ITRYETTVGRALLSEILPAGLSFELLNKPLKKKEISRLINVGFRRCGLRETVIFADKL
                     MQAGFGIATRAGISFGVNDMRIPEQKQELIKQAESEVKEIENQYTSGLVTAGERYNKV
                     VDIWSRCGEQVGKVMMEQLGQEEVENRHGKKVKQEAFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVVTEDDCATSNGVSMKALVEGGEVVEALKERILGRTLATDILHPETQDV
                     LFPAGTLIDEEGVDAIDNVGIDEVKVRTALSCDTRWGVCAKCYGRDLGRGSLVNVGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVQSHVEAKSAGTVRFTSTMRYVANAKG
                     DKVVISRSGEVIITDDNGRERERHKVPYGALLSVADGDTVRAGKQVATWDPHHRPIIT
                     EYAGTVRFEHVEEGVTVAKQIDDVTGLSTLVVIDAKRRTTATGKGQRPSVKLINEAGE
                     EVRIAGTDHAVNISFPVGAVIAVRDGQQVQVGEVLARIPQEVSKTRDITGGLPRVAEL
                     FEARSPKDAGMLAEVTGTVSFGKDTKGKQRLVITDLDKQEHEYLIAKDKHLLVHDGQV
                     VNKGEMIVDGPVDPHDLLRLLGVEELARYIIDEVQDVYRLQGVKINDKHIEVIVRQML
                     RRVEITDPGDTPFITGEQAERAEVLEENEKAEKDGKKPAVYAYMLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKRDGLRGLKENVIVGRLIPAGTGLAYHNIRKAQAAEPV
                     LAEIATAVAPETTPPATEQAPATAGGGS"
     gene            7117..7467
                     /locus_tag="VFR57_00735"
     CDS             7117..7467
                     /locus_tag="VFR57_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257932.1"
                     /translation="MAKKAKYLFIASMDVDPDKEALFNEVYDNEHIPALLKVPGVLSV
                     RRGVREPLTMFIGGEKKTIVAEGEPRYSAYYELESAEVLTSEAWGKAVELGRWPSQVR
                     PFTRNRRHVLRKLL"
     gene            7482..8054
                     /gene="folE"
                     /locus_tag="VFR57_00740"
     CDS             7482..8054
                     /gene="folE"
                     /locus_tag="VFR57_00740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0257933.1"
                     /translation="MTPNLQKIETLVRQLLVELGEDPDREGLQKTPLRVAQALKFLTN
                     GYRADVQQVINNALFTQATGGMVIVKDIEMYSLCEHHMLPFFGRCHIGYIPNGKVFGV
                     SKLARLVDVHARRLQLQERLTEDISREIMESIDAKGVGVLIEARHLCMMMRGVEKQNS
                     TMVTSSVLGVFRDHLATREEFLSLVARRTL"
     gene            8138..8260
                     /locus_tag="VFR57_00745"
     CDS             8138..8260
                     /locus_tag="VFR57_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257934.1"
                     /translation="MERLQQWRRKQEGKLGYILLWALGVPIPVLFVIFLLRGCT"
     gene            8311..8547
                     /locus_tag="VFR57_00750"
     CDS             8311..8547
                     /locus_tag="VFR57_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEU0257935.1"
                     /translation="MSKFLVALSILAAGLAGCSALDEQGISRETQGTVGGAVAGGVVG
                     SAVTGGSTIGTIGGAVVGGAVGNEAGERYDEKRK"
     gene            8609..9361
                     /locus_tag="VFR57_00755"
     CDS             8609..9361
                     /locus_tag="VFR57_00755"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEU0257936.1"
                     /translation="MGVQEKQVDRAVERAMRVDLAAAFRLAVRLDLHEGVCNHFSVML
                     PDGGTFLLNRYGLHWSEVTASNLIALDSEGRILAGEGEIEKTAFYIHSRIHLANPRAT
                     CVLHTHMPYATAITLLEGGRLEMVEQNALRFHDDIAYDDEYNGLVVDNAEGDRLARVL
                     GSKRVMFLANHGVIVVGRTVAEAFDALYYLERASRLQVLARSMGGKLKPVRSEVVRET
                     YRMILADTPKYASAHFDALKRILDREEPAYSH"
     gene            9357..9872
                     /locus_tag="VFR57_00760"
     CDS             9357..9872
                     /locus_tag="VFR57_00760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0257937.1"
                     /translation="MEVRPACPADLAAIHSIYAHHVLHGLASFEEEPPSVEELQRRYR
                     DVLDRGLPYLVAELEGAIAGYGYCAPYRARSAYRYALEDSVYVRHDATGRGVGRRLLE
                     ELIRRCEDLGYRQIIAVIGDSANAASIGVHAACGFLRVGTLRSVGFKFGRWVDSVFMQ
                     RHLGRGDSTHP"
     gene            complement(9898..10872)
                     /locus_tag="VFR57_00765"
     CDS             complement(9898..10872)
                     /locus_tag="VFR57_00765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEU0257938.1"
                     /translation="MKAVLCKQYGPPDSLAIEEVESPRPGANEVVVSVKAASLNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKEVGPGVERFKPGDRVMAFTTYGAFAEEVKTEA
                     TRLIRLPQKMDFVTGAAFLLTYGTTDHALRDRAALSSGETLLVLGAAGGVGLAAVEVG
                     KALGARVIACASTGEKLAVCRDHGADETINYATEDLRERIRALTGGRGADVVYDPVGG
                     PYSEPAFRSIAWRGRHLVVGFAAGEIPKLPLNLALLKGASVVGVFWGDFARREPARFA
                     ESVAQLARWYDEGRLKPHVDETLPLTSAREALKRMAERKVKGKLVLTT"
     gene            11541..11768
                     /locus_tag="VFR57_00770"
     CDS             11541..11768
                     /locus_tag="VFR57_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257939.1"
                     /translation="MIVIESGGTEEGLMKSVFDSSFKYTPSFDTDVRKTFQRVRRREG
                     NRPVSLVVMPSQRETQIAVRPLEQFKRARAE"
     gene            12018..12908
                     /locus_tag="VFR57_00775"
     CDS             12018..12908
                     /locus_tag="VFR57_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0257940.1"
                     /translation="MPTLSTDDGVKLYYEETGSGTPIVFVHEFGGDHRSWEPQVRYFS
                     RRYRCITFNARGYPPSDVPEDWERYSQARARDDIRCVLDALQIQRAHVVGLSMGGFAT
                     LHFGITYGYRALSITAAGAGYGSHPAQYRDFQAQSKKNAELINREGIAHFVETYGRGA
                     QRVQLEIKDPRGFEEYLRQFKEHSALGAINTLLGVQCRRPSFYDLTAEMARMDVPTLI
                     MSGDEEEPCLDTNLLMKRTIPGAALAVLPRSGHAINLEEPELFNRLLDDFFHQVEAGR
                     YTPRDLRSTVPSIYGASGKP"
     gene            12905..13684
                     /locus_tag="VFR57_00780"
     CDS             12905..13684
                     /locus_tag="VFR57_00780"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0257941.1"
                     /translation="MSLHVEKRDAVGWIVFDQPKRRNAINGAMWRGIPPAMARFSEDP
                     DVRCVAFRGAGNEAFSAGADISEFEANRARSEAVGEYDDLLDRVLHSIQGSLKPSVAV
                     IYGYCLGGGLEIALACDLRYCAASAQFGIPAAKLGLAYNVEGHKRLLETVGHARAREI
                     MFLGRRYNAEEALAMGIVHKVLPTESLEGFVAEVLQNLVDNAPLSIANSKTIIEEYVK
                     SLGTPDAARMRSAIERCAKSEDYREGRRAFMEKRKPRFTGR"
     gene            13681..14760
                     /locus_tag="VFR57_00785"
     CDS             13681..14760
                     /locus_tag="VFR57_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0257942.1"
                     /translation="MKIRSIRARAVAAPIKRPLATSVGTVSAAALMLLDLETDAGIVG
                     RSYLFAIGKHNLAPLAKLVEAMAEMVKGDEVAPFDLERKLRGRYALLGVHNIVLFAMA
                     GIDMAAWDAFAQSLELPLARVLGAAPRPILAYNSKGLGILPEKELAKEAGELVAEGFR
                     AVKLRLGRPNAAEDLRALRAVKKEIGADVTLMVDFNQGLTVAEAIRRGRMIDDEGGVY
                     WIEEPVRADDHAGCARVAREVHTPIQIGENFMGPEQMAQALAAGSCDYVMPDAERIGG
                     VTGWMRAAALAQAAGVEMSSHLFPEVSSHLLAATPTCHWLEYMDWADAILERPVEMKD
                     GHVVIPPEPGTGIRWNEKAVERYQV"
     gene            14760..16385
                     /locus_tag="VFR57_00790"
     CDS             14760..16385
                     /locus_tag="VFR57_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU0257943.1"
                     /translation="MFTRHFAHWPPGQPKTLELPRETVYGNLVATVARSPQRAAIDYY
                     GSRISYAELKRQVDVLAGFLQQRFGVARGDRVLLYAQNSPQFVIAYYAILRADAVVVP
                     VNPMNRTEELRHYIEDSGARVALAGQDVLEQLEPLGLPHMLPIVYSDYLTAASDLPIP
                     ELVRARGSGAWKEMLASGLSPAPHVAGPEDLAVMPYTSGTTGKPKGCMHTHSSVQATT
                     VAYLYWRGTQEESVVLSALPMFHVTGMQAGMNSPIHTAATIVIMSRWDRTCAAMQIER
                     ARVTNWSAITTMLVDFLSNPELEKYDLSSLRVLGGGGAAMPEALARKLEQVIGLPYVE
                     GYGLSETMAPSHINPPHRPKRQCGGIPFFNTDARVIDVETKAELGPDQVGEIIVHGPQ
                     VFRGYWKQPEATAQAFIDHDGKRFFRTGDLGYYDDEGYFFITDRLKRMINCSGFKVWP
                     AEVESMLYAHPAIQEACVIGSPDGYRGETVKALVVLRPEAKATAAQDIIDWARSRMAA
                     FKVPRTVQFVEALPKTATGKILWRELQEKENRK"
     gene            16382..16819
                     /locus_tag="VFR57_00795"
     CDS             16382..16819
                     /locus_tag="VFR57_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0257944.1"
                     /translation="MKWIATLLFFLCFSALAAEIEVRQPWARATPPGAKVAAGYLEIR
                     NNGKEADRLLSASTPVAKRVEMHITQRDGEVVKMREVKSLDIPAGGTRKLEPGGTHLM
                     LIELAQPLKQGERFRLRLRFERAGDLEADVEVRALGARQEHRH"
     gene            complement(16869..17882)
                     /locus_tag="VFR57_00800"
     CDS             complement(16869..17882)
                     /locus_tag="VFR57_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU0257945.1"
                     /translation="MEGAAGQIGLGVVIALVVVALAFDFMNGFHDAANSIATVVSTHV
                     LKPYQAVVLAAFFNFAAFFAFHLSVAATVGKGIVDQGIVDQYVVFGALIGAIAWNLIT
                     WWYGIPSSSSHALIGGLVGAAVAKAGTGALIAPGLLKTIAFIFVAPVLGMVFGALLML
                     AVAWIFVRTTPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTAGVIWMLLIASGYLGAK
                     DPLPAWVLVSCYVMIGLGTAFGGWRIVKTMGQRITKLRPVGGFCAETGGAAMLFLATN
                     LGIPVSTTHTITGAIVGVGTAQNVSAVRWGIAGDLLWAWVLTIPCSAFMAALAWALGR
                     ALL"
     gene            complement(17882..18508)
                     /locus_tag="VFR57_00805"
     CDS             complement(17882..18508)
                     /locus_tag="VFR57_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEU0257946.1"
                     /translation="MLGRLMPREGKFFDLFNAHADRILEGSRELAAMLGTFTELEAHA
                     QRIDAAERAADKVTHETIALLHKTFITPFDRDQIHQLITAMDDILDLIQDVAESIALY
                     DLRRITPEAQQLAEICQMSCERVRDTVGLLTNVKRADAIMKNCEEIDRLESDADRVMR
                     AALSKLFREESDIKQVIKLRAIYDLLERITDRCEDVANVVEGIVLENS"
     gene            complement(18511..19650)
                     /locus_tag="VFR57_00810"
     CDS             complement(18511..19650)
                     /locus_tag="VFR57_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0257947.1"
                     /translation="MGPLAGIKVLEFESIGPAPFAGMLLADMGADVLVIDRPASTDLG
                     LKRERWYDVMMRGKRSVTLDLKSNTAREAALALAGKADALIEGMRPGVMERLGVGPDE
                     ALKSNPRLVYGRMTGWGQDGPLALRAGHDINYIALAGVLHAFGRKGEAPVPPLNLIGD
                     FGGGGMLLGFGVACALIEAARSGRGQVVDAAMVEGAALLATMFAGFLKAGSWSEARGD
                     NILDTGAPWYDVYETKDRKYVSIGSIETRFYEELLQKLGVPDLGQHDRKRWPEMRERF
                     ATVFKSKTRDEWCKVFDGSDACFAPVLSWSEARRDAHNVARKAYVEVAGVEQPAPAPR
                     FSRTPAGVRRSPPERSEGGRAALADWGFDAKAIDRLRSLGLGAKE"
     gene            19668..20492
                     /locus_tag="VFR57_00815"
     CDS             19668..20492
                     /locus_tag="VFR57_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU0257948.1"
                     /translation="MPGRYPERIVCLTEESTEILYLLGEERRIAGISGYTVRPPRARR
                     EKPRVSAFLSARTDKILELKPDLAIGFSDLQADIARDLAKAGLNVLIFNQRSVQQILD
                     MILALASLVGAAKKGEALVASLEQGLEEIRAAAGRFERRPKVYFEEWDEPMISAIRWV
                     SELIEIAGGEDIFSERSHSQAAHGRIVTPDLVVSRRPDVMLGSWCGKKFRPERVAARP
                     GWQDLPAVRENRLYEIKSADILQPGPAALTDGVRRIHELLRDSGCRAARTQSSAGA"
     gene            complement(20440..21171)
                     /locus_tag="VFR57_00820"
     CDS             complement(20440..21171)
                     /locus_tag="VFR57_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU0257949.1"
                     /translation="MAWALQALAGRLIRVFRGYMASRTSAADELRRIDTVGNAFRYFA
                     SVVVVLVAGMLILNELGISIAPILATAGVAGIAIAFGAQSLIKDYFTGFFLLIEDQIR
                     QGDVVEISGKSGEVEEVTLRYVRLRDNDGFVHFVPNGEIKLVTNRTRGFGQAVMDAVI
                     KSSQSVEDAFQAMRDVGREMRQDAAFAPQILGDVQIAGIERWEHFGVFLRCRMKVLPE
                     EQWRVRAEFMRRLKIAFEQRGIQSP"
     gene            complement(21274..22101)
                     /locus_tag="VFR57_00825"
     CDS             complement(21274..22101)
                     /locus_tag="VFR57_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0257950.1"
                     /translation="MGAPEATAAISPNPATFSYEHEGDRQFATTLARGLEVLRCFTPL
                     EPLLGNKEISVRTGLPKPTVSRLTYTLTKLGYLRHNMRLGKYQLGSAVLSIGYPLLAS
                     MNVRQVARPLMKELADYCNGSVSMGVRDRLNMVYVESCRSGNSITTLPDIGTSVPISQ
                     SVIGRAFLSACTPPERESVLNQMKVKEPEAHRKFRPSIDKALDDIRSRGFCASTGELR
                     REVYAVGAPMRRTVDGEIVSFNCIVPGFMVKKGQLEDDLGPRLVAMVRNIEASLGLH"
     gene            22235..22993
                     /locus_tag="VFR57_00830"
     CDS             22235..22993
                     /locus_tag="VFR57_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0257951.1"
                     /translation="MRSLFDLAGKIALVTGATRGIGRSIAEELGKAGAKVAICSRKAD
                     ACAETEADLARSGTEVLAMPCNVSRKEELQALVDATRARWGRIDIVVANAATNPYYGP
                     LTGIGDEAFDKIFANNLKSVLWLAGMTLPGMTAGGSFILVGSIGGIIANTVIGAYGMS
                     KAAGHHLVRNLAAEWGPKNVRVNAIAPGLIKTEFARALWEDPKRAEERVSNTPLRRLG
                     EPRDIGGIAVFLASPAAAFITGQCIVADGGVTIL"
     gene            22996..24108
                     /locus_tag="VFR57_00835"
     CDS             22996..24108
                     /locus_tag="VFR57_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0257952.1"
                     /translation="MDLNYSPEELAFRDEVRSWLAANLPQELKDKAHYSKEELMRWHR
                     ILAAKGWVAPAWPKEWGGTGWSAVQRYIYEEELGYAGTPPIIPFGINMCAPVLIAFGT
                     DAQKKRFLPRIYNGEDFWCQGYSEPGSGSDLASLKTKAVKAGAHYVVNGQKIWTTLAH
                     YADWIFCLVRTDPTVEKRQEGISFLLIDMKSPGITLQPLMLMDGAHHVNEVFFDDVKV
                     PADNLVHKENQGWTVAKYLLGYERMNTGRIGESKRELRKLKERFPEKMKDVRWRDRLS
                     RVEVELMALEITNLRFLDRMRRTGQPPGADVCMLKIKGTEIQQALSELAMQAADPAAR
                     ETQALERRYADMRKTTIYAGSNEIQRNIIAKATLGL"
BASE COUNT         4543 a         7777 c         7810 g         3992 t
ORIGIN      
        1 gcaacaagct cgaagtgaga gtcgagccgc gcctcacgct ttcgccgaag gacatcgtcg
       61 aagtcatccg catcctggtc gagctgcgca acggccgcgg cgagatcgac gacatcgacc
      121 acctcggcaa ccgccgcgtg cgctcggtgg gcgagctcgc cgagaaccag ttccgcgcgg
      181 gcctggtgcg cgttgagagg gcggtgaagg aacgcctgtc ccaggcggaa tccgagaacc
      241 tgatgccgca tgacctgatc aacgcgaagc cgatttccgc cgcgatcaag gagttcttcg
      301 gctcgagcca gctctcgcag ttcatggacc agaccaaccc gctctcggag atcacgcaca
      361 agcgacgcgt ttctgctctt ggacccgggg gtcttacccg tgaacgggcg ggcttcgagg
      421 tcagagacgt acacccgacg cactacggcc gcgtgtgccc gatcgagacg ccggaaggtc
      481 cgaacatcgg cctcatcaac tcgctcgcgc tctacgcgcg caccaacaag tacggcttcc
      541 tcgagacgcc ctaccgcaag gtgaaggaca gcaaggccac gaacgagatc gtctatctct
      601 cggcgatcga agaaggcaac tacgtcatcg cccaggcgaa cgcgtcgctc gacaaggcgg
      661 gcaagctcac cgacacgctg gtgtcgtgcc gcttcaagaa cgaattcgag ctgaagtcgc
      721 ccgaggaagt gcagtacatg gatgtggcgc cgtcgcagat tgtgtccgtg gcggcctcgc
      781 tcatcccgtt cctcgagcac gatgacgcga accgcgcgct catgggctcg aacatgcagc
      841 gccaggccgt gccctgcctg cgcccggaga agcctttcgt cggcaccggc gtcgagcgca
      901 ctgcggcgat cgactcgggc acctgcgtga tcgcgctgcg cggcggcctg gtcgactacg
      961 tcgacgcgaa ccgtgtggtc gtgcgcgtga acgacgatga aaccgtggcg ggcgacgtgg
     1021 gcgtggatat ctacaagctc acgaagtaca cgcgctccaa ccagaacacc aacatcaacc
     1081 agcgcccgat cgtgaagcag ggcgagaaga tcgcgaaggg cgacgtggtc gccgatggcg
     1141 cctccacgga cctgggcgag ctcgcgctcg gccagaacat gctggtcgca ttcatgccgt
     1201 ggaatggctt caacttcgag gattcgatcc tcatctccga gcgcgtggtc gccgatgacc
     1261 gctttacctc gatccacatc gaggagctga cggtggtcgc gcgggacacg aagctcgggc
     1321 cggaagaaat cacgcgcgat atctcgaacc tgtccgaagc gcagcttgcg cggctggacg
     1381 agtcgggcat cgtgtacatc ggcgccgaag tcgaggcggg cgacgtactg gtgggcaagg
     1441 tgacgccgaa gggcgaaacc cagctcaccc cagaagagaa gctgttgcgc gcgatcttcg
     1501 gcgagaaggc gagcgatgtg aaagacacgt cgctgcgcgt tccgtctggg atctccggaa
     1561 ccgtgattga cgttcaggtc tttacgcgcg agggcatcga acgcgacaag cgcagccagg
     1621 cgatcatcga ggatgagctg aagcgctaca agaccgatct cgccgatcaa atgcgcattg
     1681 tcgaggccga tacattcgag cgcctcgagc gcctgcttct caacaagacc gcgaacggcg
     1741 gcccgaagcg tctcgccaag ggcaccaagg tgacgaagga gtacatcgac ggcgtcgaga
     1801 agtacgactg gttcgacatc cgcctcgcca acgaagagtc ggcggcgcag gtcgagggtt
     1861 tgaaggactc gctcgcgcag aagcgccgcg agttcgacgc catgttcgag gcgaagcgga
     1921 agaagctcac gcagggcgac gagctgccgc cgggcgtgct caagatggtg aaggtgtacg
     1981 tcgcggtgaa gcggcgcctg caacccggcg acaagatggc cggacgccac ggcaacaagg
     2041 gcgtcatttc caagatcacg ccggtcgagg acatgccgca catggccgac ggcacgccgg
     2101 tcgacatcgt gctcaacccg ctcggtgtgc cttcgcggat gaacgtgggg cagatcctgg
     2161 agacgcatct tggctgggcc gccaagggcc tgggcttcaa gatcggcgcg ctgatcaagc
     2221 agcagcagaa gcttgccgag ctgcgcaaga ccctcgaagc catctacagc ggccgcgacg
     2281 aggacgtctc ccagctgacg gacgaggaag tgctgcagct cgcgcagaac ctgcgcgaag
     2341 gcgtcccgtt cgccacgccg gtgttcgacg gcgccgccga gagcgagatc aacggcatgc
     2401 tcgagatggc cagcctgccc accacggggc aggtgcagct ctacgacggg cgcaccggcg
     2461 aggccttcga ccgcaacgtg accgtgggct acatgcacat gctcaagctc catcacctcg
     2521 tcgacgacaa gatgcacgcg cgctcgacgg gaccgtactc gctcgtcacg cagcagccgc
     2581 tcggcggcaa ggcgcagttc ggcggtcagc gcttcggcga gatggaggtg tgggcgctcg
     2641 aggcgtacgg cgcggcgtac acgctgcagg aaatgctcac cgtcaagtcc gacgacatca
     2701 ccggccgcac caaggtgtac gagaacatcg tcaaggggga ccacaagatc gacgcgggca
     2761 tgcccgagtc gttcaacgtg cttgtgaaag agatccgctc ccttggcatc gacatcgatc
     2821 tggagaggta ttaatgaaag cgcttctaga gctcttcaag caggtccagc aggacgagga
     2881 gttcgatgcg atcaagatcg ggctcgcatc gcccgagaag atccgctcgt ggtcctacgg
     2941 cgaggtgaag aagcccgaga ccatcaacta ccgcacgttc aagccggagc gcgacggtct
     3001 tttctgcgcg aagatcttcg ggccaatcaa ggactacgag tgcctttgcg gcaagtacaa
     3061 gcgcctcaag caccgcggcg tgatctgcga gaagtgcggc gtcgaggtga cgctcgccaa
     3121 ggtgcgccgc gagcgcatgg gccacatcga gctcgcctcg ccggtggcgc acatctggtt
     3181 cctgaagtcg ctgccctccc gtctgggtct cgttctggat atgaccctcc gagacatcga
     3241 gcgggtgctc tatttcgaag cctatgtggt taccgacccg ggcctcaccc cgctcaagcg
     3301 cacccagctc ctcaccgagg atgactacct cgcgaaggtc gaggagttcg gggacgactt
     3361 ttccgcggcc atgggcgccg agggtatccg cgcgctgctg cgctcgctcg acctgaacaa
     3421 ggcgatcgac gagctgcgca aggatctcga gggcaccacg tcggatgcca agatcaagaa
     3481 gatcgccaag cggctcaagg tgctcgaggg cttcaagtcc tcgagcatca agcccgactg
     3541 gatgatcatg gaagtgctcc cggtgctgcc gcccgagctg cggcctctcg tccccctgga
     3601 cggcggacgc ttcgccacgt cggatctgaa cgatctctac cgccgcgtca tcaaccgcaa
     3661 caacagactt aaaagattat tggagttaaa ggcgccggag atcatcgtcc ggaacgagaa
     3721 gcgcatgctg caggaggcgg tggactcgct cctcgacaac ggccgccgcg gcaaggcgat
     3781 gaccggcgcg aacaagcgcc cgctcaagtc gctcgccgac atgatcaagg gcaaaggcgg
     3841 ccgcttccgc cagaacctgc tcggcaagcg cgtcgactac tccggccgct cggtgatcgt
     3901 ggtcggcccg cagctcaagc tccatcagtg cggcctgccg aagaagatgg cgctcgagct
     3961 attcaagcct ttcatcttca acaagcttga gctgctcggt ttggccacca ccatcaaggc
     4021 cgccaagcgg ctggtcgaac aggaagtcgc cgaagtgtgg gacatcctgg aagaggtgat
     4081 ccgcgagcat ccggtgcttc tcaaccgcgc gccgacgctg caccgcctcg gcatccaggc
     4141 attcgagccg gtgctcatcg aaggcaaggc gatccagctg catccgctcg tctgcgcggc
     4201 gttcaacgcc gacttcgacg gcgaccagat ggcggttcac gtgccgctgt cgctcgaggc
     4261 gcagatggaa gcgcgtacgc tcatgctcgc ctcgaacaac gtgctttcgc cggcgaacgg
     4321 cgaaccgatc atcgtgccct cgcaggacat cgtgcttggc ctctactacg cgacccgcga
     4381 gcgcatcaac gcgaagggcg agggcatgag cttctccgac cttgccgaag tgcaccgcgc
     4441 ttacgactcg cgccaagtgg agctgaacgc gcgcatctgg gtgcgcatca aggagaagga
     4501 gctgtccgac tccggcgagc agaccgagaa gatcacgcgc tacgagacca ccgtcgggcg
     4561 cgcgctgctc tccgagatcc tgcccgccgg gctttcgttc gagcttctga acaagccgct
     4621 caagaagaag gagatctcga ggctcatcaa cgtcggcttc cgccgctgcg gtctgcgcga
     4681 gaccgtgatc ttcgccgaca agctcatgca ggcgggcttc ggcatcgcca cgcgcgccgg
     4741 catttcgttc ggcgtgaacg acatgcgcat cccggagcag aagcaggagc tcatcaagca
     4801 ggccgaatcc gaggtaaagg aaatcgagaa ccagtacacc tccggtctcg tcaccgcggg
     4861 cgagcgctac aacaaggtgg tcgacatctg gtcgcgctgc ggcgagcagg tcggcaaagt
     4921 gatgatggag cagctcggcc aggaagaggt cgagaaccgc cacgggaaaa aggtcaagca
     4981 ggaagccttc aactcgattt acatgatggc cgactcgggc gcgcgcggct cggcggcgca
     5041 gattcgtcag ctcgccggta tgcgcggcct gatggccaag cccgacggct cgatcatcga
     5101 aacgccgatc acggcgaact tccgcgaagg cctgaacgtg ctgcagtact tcatctcgac
     5161 gcacggcgcg cgcaaaggtc ttgccgacac cgcgctcaag acggcgaact cggggtatct
     5221 cacccggcgc ctcgtcgacg tgacgcagga cctcgtggtt accgaagacg attgcgcgac
     5281 ttcgaacggc gtgtcgatga aggcgctcgt cgagggcggc gaggtcgtcg aagctctgaa
     5341 ggagcgcatc ctcggccgca cgcttgccac cgacatcctg catcctgaga cgcaagatgt
     5401 gctcttcccc gccggcaccc tcatcgacga ggaaggcgtc gacgcaattg acaacgtcgg
     5461 catcgacgag gtcaaggtgc gcactgcgct ttcgtgcgat acgcgctggg gagtctgcgc
     5521 caagtgctac ggacgcgatc tcggccgcgg ttcgctcgtg aacgtgggcg aggcgatcgg
     5581 cgtgatcgcc gcgcagtcga tcggcgagcc gggcacgcag ctcaccatgc gcacgttcca
     5641 catcggcggt gcggcatccc gaaccgcggt gcagagccac gtggaggcca aatctgccgg
     5701 cacggtgcgc ttcacttcga ccatgcgcta cgtcgcgaac gctaaaggcg acaaggtcgt
     5761 gatcagccgc tcgggcgaag tgatcatcac cgatgacaac ggccgcgagc gcgagcgcca
     5821 caaggtgccg tatggcgcgc tcctttccgt cgccgacggc gatacggtgc gcgcgggcaa
     5881 gcaggtcgcg acctgggatc cgcaccaccg tccgatcatt accgagtacg ccggcacggt
     5941 gcgcttcgag cacgtcgaag aaggcgtaac ggtggcgaag caaatcgacg acgtcaccgg
     6001 cctttcgacc ctcgtcgtga tcgacgccaa gcgccgcacc acggccacgg gcaagggcca
     6061 gcgtccctcg gtcaagctca tcaacgaggc gggcgaggaa gtgcgcattg ccggcaccga
     6121 tcacgccgtc aacatcagct tcccggtggg cgcggtgatc gcggtgcgcg acggccagca
     6181 ggtgcaggtg ggtgaagtac tcgcgcgcat cccgcaggaa gtctcgaaga cgcgcgacat
     6241 cacgggcggc ctgccgcgcg tggccgagct cttcgaagcg cgctcgccga aggacgcggg
     6301 catgctcgcg gaagtgaccg gcacggtgag ctttggcaag gacaccaagg gcaagcagcg
     6361 cctggtgatc acggacctcg acaagcagga gcacgagtac ctgatcgcca aggacaagca
     6421 cctcctcgtg cacgacggcc aggtcgtcaa caagggcgag atgatcgtcg acggtccggt
     6481 ggatccgcac gacctgctgc gcctcctcgg cgtggaagag ctcgcgcgct acatcatcga
     6541 cgaggtgcag gacgtgtatc gcctgcaggg cgtgaagatc aacgacaagc acatcgaggt
     6601 gatcgtgcgc cagatgctgc ggcgcgtgga gatcaccgat ccgggcgaca cgccgttcat
     6661 caccggcgag caggccgagc gcgccgaagt gctcgaggag aacgagaagg cggagaagga
     6721 cggcaagaag ccggcggtgt acgcctacat gctgctcggc atcaccaagg cctcgctctc
     6781 gaccgactcg ttcatctccg cggcatcctt ccaggagact acgagagtac tcaccgaggc
     6841 ggcgatcatg ggcaagcgcg acggcctgcg cggcctcaag gagaacgtca tcgtcgggcg
     6901 cctcatcccg gcgggaaccg gtctcgccta ccacaacatc cgcaaggccc aggccgcgga
     6961 gccggtgcta gctgagatcg cgacggccgt cgcgccggag actacgccgc cggcaaccga
     7021 gcaggctccc gcaacagctg gcggcggcag ctagccggtt tttccaacta acaaaggcgc
     7081 cttcgggcgc cttttctttt ccagagagga gagccgatgg cgaagaaagc gaaatacctc
     7141 ttcatcgcga gcatggacgt cgatccggac aaggaggcgc tcttcaacga ggtgtacgac
     7201 aacgagcaca tcccggcgct gctcaaggta ccgggcgtgc tttccgtgcg ccgcggcgtg
     7261 cgcgaaccgc tcaccatgtt cattggcggg gagaagaaga ccatcgtcgc cgaaggagag
     7321 ccgcgctaca gcgcctacta cgagctcgag agcgccgagg tcctgacaag cgaagcgtgg
     7381 ggtaaggccg tggagcttgg ccggtggcct tcgcaggtgc gccccttcac tcgcaaccgc
     7441 cgccacgtgc tgcgcaagct tctctgaaat cgtcggctgc catgacaccg aacctgcaga
     7501 agatcgagac gctcgtccgc cagctgctcg tcgagctcgg cgaggatccg gaccgcgagg
     7561 gactgcagaa gacgccgctg cgcgtcgcgc aggcgctcaa gttcctgacg aacggctatc
     7621 gcgccgacgt gcaacaggtg atcaacaacg cgctcttcac ccaggcgacg ggcggcatgg
     7681 tgatcgtcaa ggacatcgag atgtacagcc tgtgcgagca ccacatgctt ccgttcttcg
     7741 gccgctgcca catcggctac atcccgaacg gcaaggtgtt cggcgtaagc aagctcgcgc
     7801 gcctcgtcga cgtccacgcg cgccgcctgc agctccagga gcggctcacc gaagacatct
     7861 cacgcgagat catggagtcg atcgacgcca agggcgtcgg cgtgctgatc gaagcgcgcc
     7921 acctctgcat gatgatgcgc ggcgtcgaga agcagaattc gacgatggtg acgtcgtccg
     7981 tgctcggcgt tttccgcgac cacctcgcca cccgcgagga gtttctgagc ctcgtagccc
     8041 ggcgcacgct gtaatgaatt ccccgacagc ggcctagaaa tcccggcacg ccgtttgctg
     8101 ccctccggta tcggctcaaa cgagcacgga ggtaggtatg gaaaggttgc aacagtggcg
     8161 ccggaagcag gaaggcaagc ttggctacat cctgctgtgg gcccttggcg tgcccatccc
     8221 ggtcctgttc gtcatcttcc tgctgcgcgg ctgcacctga gcaccgagca cagggtcgtt
     8281 cttctttttt cttcaaccgg aggccatgac atgagcaagt ttcttgttgc attgagcatt
     8341 cttgcggccg gcttggcggg ctgctccgcg ctggacgaac aaggcatctc gcgcgagaca
     8401 cagggtacgg tcggcggcgc tgtcgccggc ggcgtcgtcg gctccgcggt gactggcggc
     8461 agcacgatcg gcacgatcgg cggcgcggtg gtcggcggcg cggtcggcaa cgaagcgggc
     8521 gagcgctatg acgagaagcg taagtaagag catctcttga ccgattgaag ggcgctcttg
     8581 cggcgccctt tctttttata atggccgcat gggagtccaa gaaaagcagg tcgacagagc
     8641 ggtcgagcgc gccatgcgcg tcgacctcgc cgcggctttc cgcctcgcag tgcgattgga
     8701 tctgcacgaa ggcgtctgca accactttag cgtcatgctg cccgatggcg gaaccttcct
     8761 cctcaatcgc tacgggcttc actggagcga ggtcaccgcg tccaacctga tcgcgctgga
     8821 ctccgaaggc aggatcctcg ccggtgaggg agaaatcgag aagacggcgt tctacatcca
     8881 ctcgcgcatc catctcgcga atccgcgggc aacctgtgtt ctgcatacgc acatgccgta
     8941 tgccaccgcg atcacgctac tcgaaggcgg acgcctggag atggtggagc agaacgcgct
     9001 gcgcttccac gacgatattg cctacgacga cgagtacaac ggcctcgtcg tcgacaacgc
     9061 ggaaggcgac cgtctcgcgc gggtgctcgg ctccaaacgt gtcatgtttc tcgccaacca
     9121 tggtgtgatc gtcgttggcc gcaccgtggc cgaggctttc gacgcgctct attacctcga
     9181 acgcgcgtct cggcttcagg tccttgcgcg ctcgatgggc gggaagctga agccggtgcg
     9241 ctccgaagtc gtgcgggaaa cgtatcggat gatcctggcc gacacgccca aatacgccag
     9301 cgcccatttc gacgcgctga agcgcatcct cgaccgcgag gagccggctt attcccattg
     9361 aagtacggcc ggcctgcccc gccgacctcg ccgcaatcca ctcgatctac gcgcatcacg
     9421 tacttcatgg gctcgcttcg ttcgaggagg agcccccgtc ggtggaggag ctgcagcggc
     9481 gctatcgcga cgttctagac cggggcctgc cataccttgt cgccgagctc gagggcgcaa
     9541 tcgccggcta cggctactgc gcgccgtacc gcgcgcgcag cgcctaccgc tacgcgctcg
     9601 aagattcggt ctacgtgcgc cacgacgcga ccggccgcgg cgtgggccgg cgcctcctgg
     9661 aggagcttat ccggcgctgc gaggacttgg gctaccgcca gatcatcgcg gtgatcggcg
     9721 acagcgcaaa cgccgcgtcc atcggtgtgc atgcagcctg cggatttctg cgcgtcggca
     9781 ccctgcgctc ggtcggcttc aagttcggcc gctgggtcga cagcgtgttc atgcagcgcc
     9841 atctcggccg cggggactcc acccacccgt agcgaaatcg ggctctgacc ccgattttca
     9901 ggtggtgaga accagcttgc ctttcacctt gcgctcggcc atgcgcttga gcgcctcgcg
     9961 cgcgctcgtg agcggcaacg tttcgtcgac gtgcggtttg aggcggcctt cgtcgtacca
    10021 gcgcgcaagc tgcgccacgc tctcggcgaa gcgcgccggc tcgcgccgcg cgaagtcgcc
    10081 ccagaacaca ccgaccacgg aagcgccttt caggagcgct aggttgagcg gcagcttggg
    10141 aatctcgccg gcggcgaagc cgaccaccag gtggcggccg cgccacgcga tcgagcggaa
    10201 ggccggctcc gaatacgggc cgccgaccgg atcgtagacc acgtccgcgc cgcggccccc
    10261 ggtgagcgcg cggatgcgct cgcgcagatc ttcggtggcg tagttgatcg tctcgtcggc
    10321 gccgtggtcg cggcataccg cgagcttttc accggttgac gcgcacgcaa tgacgcgtgc
    10381 gccgagcgct ttgccgacct ccaccgccgc aagcccgacg ccgcccgccg cgccgagtac
    10441 gagcagcgtt tcaccgctgg agagcgctgc gcggtcgcgc agcgcgtgat ccgtcgtgcc
    10501 gtaggtgagg aggaaagcgg cgccggtgac gaaatccatt ttttgcggca gccggatcag
    10561 gcgcgtggcc tcggtcttca cttcctcggc gaacgcgccg taggtggtga aggccatgac
    10621 gcggtcgccc ggtttgaagc gctcgacgcc cgggccgact tccttcacca cgccggccag
    10681 ctcgctgccg ggcgagaacg gcagcggcgg cttgaactga tacttgttct ggatgatgag
    10741 cacgtccggg aagttgaggc tcgccgcttt caccgagacg accacttcgt tcgcgcccgg
    10801 gcgcggcgat tccacttctt cgatcgcgag cgaatcgggc ggaccgtact gtttgcagag
    10861 caccgctttc atcgtggatg agcattatct cagcccgatg agaaagaccg gcccgaatgc
    10921 cgttcactac aagaggagga tgacaaccgc gacgacggct aaagcggcca gcacttcgat
    10981 cacgcgactt gtgaggctcg gcaggacgct ttgatgcagt ttgtatttgt ccatcggtaa
    11041 cctcccgaag gcaccgtagc aaaccaatgc cggacgcgct gttagatcgg tcacgggccc
    11101 ctataactcg gcggttcttc cccgacatgt actagggctt tcccttattg atctgcgtct
    11161 tctcacagcc acaatagttc ggtaggggga aacggaggag ccatgcacga gtttagagag
    11221 tgcttcatcc gcgacccttc gggcgcgtgg cggtgcgtaa agccagccga tattcaactg
    11281 ccaactggga gggtccaagt aaccgccggc agcgtattca ctagaagcac ccagttcatg
    11341 aacgtcgacc tcgcggcttt gctggacgag caagaggcgc tcgagagacc cacgtaacag
    11401 gcaccggcca caaccagcta gggcgactcg gttcttcggc ggcccggcat gggcgcggct
    11461 tttccttgac tctcggcgag cggattcgcc tgtagagcca tcgcgacacg cgtatcagga
    11521 ggcactgact tgaaacctgg atgattgtga tcgagagtgg cggaacagag gaggggttaa
    11581 tgaaaagcgt attcgattca tcgttcaaat acacgccaag cttcgatacc gacgtgcgca
    11641 agaccttcca aagggttcgt cgccgcgaag gtaataggcc cgtcagcctg gtagtcatgc
    11701 cgagccagcg tgagacccag attgcggtgc ggccgttgga gcagttcaag cgggcgcgcg
    11761 cagagtagcg cgagcggtac aagagggacg gtgcttacgc accggccctg cagcgcctcg
    11821 agcagccgag gacatcacta gtacaactgc cagcgcccaa cgtcgctaat aagcgcgcga
    11881 gctgaccgtc accgcagtcg ccgaccagct cggacgtttc gaagaagccg ggctagtaca
    11941 ctgtgccggc atcctgcctg tttcacctac atgcatattt tcaagtactt atataggacg
    12001 taggctacat ttgcgttatg ccgaccctct ccaccgacga cggcgtcaag ctctattacg
    12061 aggaaaccgg tagcggcacg ccgatcgtgt tcgtgcacga gttcggcggg gaccaccgca
    12121 gctgggagcc gcaggtgcgc tatttctcgc gccgctatcg ctgcatcacc ttcaacgcgc
    12181 gcggctatcc accgtctgat gtgcccgagg actgggagcg ctactcgcag gcacgtgcgc
    12241 gcgacgacat ccgctgcgtg ctggacgcgc tccaaattca gcgcgcgcat gtcgtggggc
    12301 tgtcgatggg cgggttcgcg acgctgcact tcggcatcac ctacggttac cgggctcttt
    12361 cgattacagc ggccggagcc gggtacggct cgcatccggc gcaataccgc gacttccagg
    12421 cgcaatcgaa gaaaaacgcg gagctcatca accgcgaagg catcgcgcat ttcgtcgaga
    12481 cctacgggcg cggcgcgcag cgcgtgcagc tcgagatcaa agacccgcgc ggcttcgagg
    12541 aatacctgcg ccagttcaag gagcactcgg cgctcggcgc gatcaacacg ttactcggcg
    12601 tgcagtgccg ccggccgtcg ttctacgacc tgactgccga aatggcgcgc atggatgtgc
    12661 cgacgctcat catgtccggc gatgaggagg agccttgcct tgatacgaac ctcctcatga
    12721 agcgcaccat tcccggcgcg gcgctggccg tcctgccgag gagcggtcat gcgatcaacc
    12781 tggaggagcc cgagctcttc aatcgcctcc tcgacgactt cttccaccag gtggaggcgg
    12841 gccgctatac gccgcgcgat ctgcgctcga ccgtgccgtc gatttacgga gcttctggca
    12901 aaccgtgagc ttgcatgtgg aaaagcgcga cgcagtcggc tggattgtgt tcgatcagcc
    12961 caagcgccgc aacgcgatca acggcgcgat gtggcgcggc atccctccgg cgatggcgcg
    13021 cttcagcgaa gatccggatg tacgctgcgt cgccttccgg ggcgcgggca acgaggcatt
    13081 ttcggccggc gccgacatct ccgagttcga ggctaaccgc gcgcgtagcg aggcggtcgg
    13141 cgagtacgac gatctcctcg atcgcgtgct gcactcgatc cagggctccc ttaagccttc
    13201 ggttgccgtg atctacggat attgcctcgg cggcggcctg gagatcgcgc tcgcctgcga
    13261 cctgcgctac tgtgcagcct cggctcagtt cggcatcccg gccgcgaagc tcggcctcgc
    13321 ctataacgtg gagggccata agcgcctcct cgagacggtg gggcacgcgc gcgcccggga
    13381 aatcatgttc ctcggccggc gctacaacgc cgaggaggcg ctcgccatgg gaatcgtgca
    13441 caaggttctg ccgaccgaat ctctcgaggg cttcgtggct gaggtgctgc aaaacctcgt
    13501 cgacaatgcg cccctctcga ttgcgaattc caagaccatc atcgaggagt atgtgaaatc
    13561 gcttggcacg cccgacgcgg cgcgcatgcg ctccgcgatc gagcgctgtg cgaaaagcga
    13621 agactaccgc gagggccggc gcgcattcat ggagaagcgc aagccgcgct tcaccggccg
    13681 atgaagattc gctcgattcg cgcgcgcgcg gtagcggcgc cgatcaagcg gccgctcgcg
    13741 acaagcgtcg ggacagtgag cgccgcagcg ctgatgctcc tcgacctcga aaccgacgcg
    13801 ggaatcgtcg ggcgctcgta cctcttcgcg atcggaaaac acaatctcgc gccacttgcg
    13861 aagctcgtcg aggcaatggc cgagatggtg aaaggggacg aggtcgcgcc gttcgatctc
    13921 gaacgcaagc tccgcgggcg ctacgcgctt ctcggcgtgc acaacatcgt tcttttcgcc
    13981 atggcgggca tcgacatggc cgcctgggac gccttcgccc agtcactcga gctgccgctc
    14041 gcgcgcgtgc tcggcgccgc gccgcggccg atcctcgcct acaactcgaa gggcctcggc
    14101 atcctgccgg aaaaggagct cgccaaggaa gcgggcgagc ttgtggccga gggtttccgc
    14161 gccgtgaagc tgcgccttgg ccgacccaat gccgcggagg atctgcgtgc gctgcgcgcg
    14221 gtgaagaagg agatcggcgc cgacgtcacc ctgatggtcg acttcaacca gggactgacg
    14281 gtcgccgagg cgatccgacg cggccgcatg atcgacgacg aaggcggcgt ctactggatc
    14341 gaggagccgg ttcgcgccga cgatcatgcg ggttgcgcgc gcgtggcgcg cgaagtgcac
    14401 acgccaatcc agattggcga gaacttcatg ggccccgagc agatggcgca ggcgctcgcg
    14461 gcggggtcct gtgattacgt catgccggat gccgagcgca tcggcggggt gacgggctgg
    14521 atgcgcgcgg ccgcgctcgc gcaagccgcg ggcgtggaga tgtcgagcca cctatttcca
    14581 gaggtgagct cgcaccttct cgccgccaca ccgacgtgcc attggctcga gtacatggat
    14641 tgggccgatg cgattctcga gcgcccggtt gagatgaagg acgggcacgt cgtcatcccg
    14701 ccggagccgg gcaccggcat ccgctggaac gagaaggcgg tcgagcgcta ccaggtgtga
    14761 tgtttacgcg gcacttcgcg cattggccgc ccggccagcc gaaaaccctg gagctgccgc
    14821 gcgagaccgt ttacggcaac ctggtcgcga ccgtcgcccg ctcgccccag cgcgcggcca
    14881 tcgattacta cggaagccga atctcctacg cggagctgaa gcgtcaggtc gacgtgctcg
    14941 ccggctttct gcagcagcgc ttcggcgtcg cgcgcggcga ccgcgtgctc ctctacgcgc
    15001 agaacagccc gcaattcgta atcgcctact acgcgatcct gcgtgccgat gcggtcgtag
    15061 tgccggtgaa tccgatgaac cgtacggaag agctgcgcca ctatatcgag gattccggtg
    15121 cgcgcgtcgc gctcgccggg caggatgtgc tcgagcaact cgagccgctc ggcttgccgc
    15181 acatgctgcc gatcgtctac tcggattacc tcaccgccgc gagcgatctg ccgattccgg
    15241 agctcgtgcg cgcgcgcggt tcgggggctt ggaaagagat gctcgcatcc gggctttcac
    15301 ccgcgccgca tgtcgcgggt ccggaggacc tcgcggtgat gccctacacc tccggcacca
    15361 ccggcaagcc gaagggctgt atgcatacgc actcgagcgt ccaggccacc accgtggcgt
    15421 acctctactg gcgtggcacg caggaagaaa gcgttgtgct ttcggcgctg ccgatgtttc
    15481 acgtcaccgg catgcaggcc ggcatgaact cgccgatcca caccgcggcg accatcgtga
    15541 tcatgtcgcg ctgggaccga acctgcgcgg cgatgcagat cgagagggcg cgggtgacga
    15601 actggtcggc gatcaccacg atgctggtcg acttcctctc gaaccccgaa ctggagaagt
    15661 acgatctttc gtcgctgcgc gtgctgggcg gcggcggggc ggcgatgccg gaagcgctcg
    15721 cgcgcaagct cgagcaggtc atcggccttc cctatgtgga aggctacggc ctctcggaga
    15781 ccatggcgcc ttcgcacatc aacccaccgc accggccgaa acggcagtgc ggcggcattc
    15841 ctttcttcaa caccgatgcg cgcgtcatcg acgttgaaac gaaagcagaa ctcggacccg
    15901 accaggtcgg cgaaatcatc gtgcacggcc cgcaggtctt ccgcggctat tggaagcagc
    15961 cggaggcgac cgcgcaggcg ttcatcgacc acgacggcaa gcgcttcttt cgcaccggcg
    16021 acctcggcta ttacgacgac gaaggttact tcttcatcac cgatcgcctg aagcgcatga
    16081 tcaactgctc cggcttcaag gtgtggccgg ccgaggtcga atccatgctg tatgcccacc
    16141 cggcgattca ggaggcgtgc gtgatcggct cgcccgacgg ctatcgcggc gaaacggtga
    16201 aggcgctcgt cgttcttagg cccgaggcga aagccactgc ggcgcaggac atcattgact
    16261 gggcgaggag ccgtatggcg gcgttcaagg tgccgcgcac cgttcaattc gtcgaggcgc
    16321 tgccgaagac cgcgaccggc aagattcttt ggcgcgagct gcaggaaaag gagaaccgca
    16381 agtgaaatgg atcgctactc tgcttttttt cctgtgcttc tcggcgctcg ctgccgagat
    16441 tgaggtgcgc cagccgtggg cgcgcgccac accgccaggc gccaaggtcg cggccggcta
    16501 tctcgagatc cgcaacaacg gcaaggaggc cgaccgtctg ctatcggcgt ccacgccggt
    16561 ggcgaagcgt gtcgagatgc acataacgca gcgagacggc gaggtggtaa agatgcggga
    16621 ggtgaagtcg ctcgacattc cggcgggcgg cacccgcaag ctcgagcccg gcggcacgca
    16681 tctcatgctc atcgaacttg cccagccgct caagcagggc gagcgattcc gcctccggct
    16741 gcgcttcgag cgcgccgggg acctcgaggc cgacgtggaa gtacgcgctt tgggcgcgcg
    16801 gcaggagcac cgccactagc gccgaggagt ccggcacttt ttccagaaaa agggtcctga
    16861 cccctttttc aaagcagcgc gcgcccgagc gcccaggcaa gcgccgccat gaacgcggag
    16921 cacggaatcg tgagcaccca tgcccacagc aggtcgccgg cgattcccca gcgcactgcc
    16981 gagacgttct gcgccgtgcc gacgccgacg atggcgccgg tgatcgtgtg cgtggtggaa
    17041 accgggatgc cgagattcgt cgcgaggaag agcatggccg cgccgccagt ctcggcgcag
    17101 aaaccgccca ccgggcgcag cttggtgatg cgctggccca tcgtcttgac gatgcgccag
    17161 ccgccgaacg cggtgcccag cccgatcatc acgtagcagc tcaccagcac ccacgcgggc
    17221 agcgggtcct tggcgccgag atagcccgac gcaatgagca gcatccagat gacgccggcg
    17281 gttttctgcg catcgttgcc gccgtggccg aggctgtaga gcgacgccga gacgagctgc
    17341 agccgccgaa accagcggtc gacgcgcgag ggcgtggtgc gcacgaagat ccacgccacc
    17401 gccagcatga ggagggcgcc gaacaccatg ccgagcaccg gtgcaacgaa gatgaaggcg
    17461 attgtcttga ggagccccgg tgcgatcagc gccccggtgc ccgctttggc gacggcagcg
    17521 cccaccaggc cgccgatcag cgcgtgggaa gaagacgatg gtatgccgta ccaccaggta
    17581 atcaggttcc acgcgatagc gccgatcaac gcgccgaata ccacgtactg gtcgacgatg
    17641 ccctgatcga cgataccctt gccgaccgtc gcggcgacgc tcaggtgaaa ggcgaagaag
    17701 gcggcgaagt tgaagaaggc cgcgagcacc accgcctgat aaggcttcaa gacgtgcgtc
    17761 gagacgaccg tggcgatcga gttcgccgcg tcgtgaaaac cgttcatgaa gtcgaaggcg
    17821 agcgccacga ccaccagcgc gatcacgacc ccgaggccga tctgcccggc cgcgccctcc
    17881 atcacgagtt ctcgaggacg atgccctcga cgacgttcgc gacgtcctcg cagcggtcgg
    17941 tgatccgctc gagaaggtcg tagatcgcgc gcagcttgat cacctgcttg atgtcgctct
    18001 cctcgcgaaa gagcttggaa agggcggcgc gcatcacgcg gtcggcgtcc gactccaggc
    18061 ggtcgatctc ctcgcaattc ttcatgatgg cgtcggcgcg cttcacgttg gtgagcaggc
    18121 ccaccgtgtc gcgcacgcgc tcgcagctca tctggcagat ctcggcgagc tgctgcgcct
    18181 ccggcgtgat gcgccgcagg tcgtaaagcg cgatcgactc ggccacgtcc tggatcaggt
    18241 ccaggatgtc gtccatcgcg gtgatgagct ggtggatctg gtcgcggtcg aatggcgtga
    18301 tgaacgtctt gtgcaggaga gcgatcgttt cgtgcgtcac cttgtcggcg gcgcgctccg
    18361 cggcgtcgat gcgctgcgca tgcgcctcga gctcggtgaa cgtgccaagc atcgcggcga
    18421 gctcgcggct gccctcgagg atgcggtcgg cgtgcgcgtt gaagaggtcg aagaacttcc
    18481 cctcgcgcgg catcaggcgt cccagcatgg ctactccttg gctccgaggc cgagggacct
    18541 cagcctgtcg atagccttcg cgtcgaagcc ccagtcggcg agcgccgcgc gtccgccctc
    18601 gctgcgctcc ggcggcgagc ggcgcacgcc ggcgggggtg cgcgagaagc gcggcgccgg
    18661 cgcaggctgc tctacgcccg cgacttccac gtaggccttc ctcgcaacgt tatgcgcatc
    18721 gcggcgcgcc tcggaccagg agaggaccgg ggcaaagcag gcgtcggagc cgtcgaacac
    18781 cttgcaccat tcgtcgcgcg tcttgctctt gaaaaccgtc gcaaaacgct cgcgcatctc
    18841 cggccagcgt ttgcggtcgt gctgcccgag gtcaggcacg ccgagctttt gcaaaagctc
    18901 ctcgtagaaa cgcgtctcga tggagccgat tgagacgtac ttgcggtcct ttgtctcgta
    18961 cacgtcgtac cagggagcgc ccgtatcgag gatattgtcg ccccgcgctt cgctccagga
    19021 accagccttg agaaaaccgg caaacatggt ggcaagaagc gcagcgcctt ccaccatcgc
    19081 cgcatcgacc acctggcccc tcccggaacg cgccgcttcg atgagcgcgc aggcgacgcc
    19141 gaagccgagc agcatgccgc cgccaccgaa atctccgatg aggttgagcg gcggcaccgg
    19201 cgcttcgccc ttgcgcccga acgcgtgcaa gacgccggca agcgcgatgt aattgatgtc
    19261 gtgtcccgcg cgcaacgcta gcgggccgtc ctgcccccag ccggtcatgc gtccgtagac
    19321 cagcctcgga ttgctcttca acgcctcgtc ggggccaacg ccgagccgct ccatcacgcc
    19381 ggggcgcata ccttcgatga gggcatccgc cttgccagcc agcgcaagcg ccgcctcacg
    19441 cgcggtgttc gatttcaagt ccaaagtcac cgagcgcttg ccgcgcatca tcacgtcgta
    19501 ccagcgctcg cgtttgaggc caagatccgt gctcgccggc cgatcaatga ccagcacgtc
    19561 ggcgcccatg tcggcgagga gcatgccggc gaacggcgct ggcccgatcg actcgaactc
    19621 cagcaccttg atcccggcga gcggccccat ggtttgataa tacgggaatg cctgggcgtt
    19681 atcccgagcg catcgtctgc ctcaccgaag agagcaccga gatcctctat ctcctgggcg
    19741 aggagcgtcg catcgccggt atctccggct acacggtgcg gccgccgcgc gcgcggcgcg
    19801 agaagccgcg cgtgagcgcg ttcctttcgg cgcgcacgga caaaatcctc gagctcaagc
    19861 ccgatctcgc gatcggcttt tccgatctcc aggccgacat tgcgcgcgat ctcgcaaaag
    19921 ccggcttgaa cgtgctcatc ttcaaccagc gctcggtgca gcaaatcctc gacatgatcc
    19981 tcgcgctcgc ttcgctcgtc ggtgcggcga agaagggcga ggcgcttgtc gcgtccctcg
    20041 agcagggcct ggaggagatt cgcgccgcgg ccggccggtt cgagcgacgg ccgaaggtct
    20101 atttcgagga atgggatgag ccgatgatca gcgccatccg ctgggtgagc gagctcatcg
    20161 agatcgccgg cggcgaggac atcttctcgg agcgctcgca ctcgcaggcg gcgcacggcc
    20221 gaatcgtgac tccggatctc gtggtgtcgc gacggccgga cgtgatgctc ggctcttggt
    20281 gcggaaagaa gttcaggccc gagcgggtcg cggcccggcc gggctggcaa gacctaccgg
    20341 cggtgcggga aaaccggctc tacgagatca agtcagcgga tattctccag ccgggtccgg
    20401 ccgcgctcac cgacggcgtg cggcgcatcc atgaactgct cagggactct ggatgccgcg
    20461 ctgctcgaac gcaatcttca gccggcgcat gaactccgcg cgcacacgcc actgctcctc
    20521 cggcagcacc ttcatgcggc agcgcaggaa cacgccgaaa tgctcccagc gctcgatgcc
    20581 ggcgatctgc acgtcgccga ggatctgagg cgcgaacgcg gcatcctgac gcatctcgcg
    20641 gccaacatcg cgcatggcct ggaaggcgtc ctcgacgctc tggctgctct tgatgaccgc
    20701 atccatcacg gcctgcccga agccgcgtgt gcggttggtc acgagcttga tctcgccgtt
    20761 cggcacgaag tgcacgaagc cgtcgttgtc gcgcagccgc acgtagcgca gcgtcacttc
    20821 ctcgacttcg ccgctctttc ccgaaatctc gaccacatcg ccctggcgga tctgatcttc
    20881 gatgagcagg aagaagccgg tgaagtagtc tttgatcagg ctttgcgcgc cgaacgcgat
    20941 ggcgatgccg gcgacgccgg cggtggcgag gatcggcgcg atcgagatgc cgagctcgtt
    21001 gaggatgagc atcccggcga cgagcaccac gacaacggag gcgaagtagc gaaacgcgtt
    21061 gccgacggtg tcgatgcggc gtaactcgtc cgccgcgctc gtgcggctcg ccatgtagcc
    21121 gcggaacacg cggatgaggc gcccggcgag cgcctgcagc gcccatgcca cgaccaggat
    21181 gacgaagatg cgccacaggc gcgtcgtggc gtaccaggcc tcgtcgtcga ggcttgcgac
    21241 gtactgcgct aggtcttcga actcttccag cggctagtgc agccccagcg aagcctcgat
    21301 gttcctcacc atcgccacta acctcgggcc aagatcatcc tcgagctgcc ccttcttcac
    21361 catgaagccc ggcacgatgc agttgaagga cacgatttcg ccgtcgaccg tgcggcgcat
    21421 tggcgcgccg accgcataaa cctcgcggcg cagctcgccg gtgctcgcgc agaacccgcg
    21481 cgagcggatg tcgtcgagcg ccttgtcgat cgacgggcgg aacttgcggt gcgcctccgg
    21541 ttccttcacc ttcatctggt tgagcactga ctcgcgctcg ggcggggtac acgccgaaag
    21601 gaacgcgcgg ccgatcaccg actgagaaat cggaacggag gtgccgatat ccggaagcgt
    21661 agtgatgctg tttccgctcc ggcaggactc gacgtacacc atgttgaggc gatcgcgcac
    21721 gcccatggag actgaaccat tgcagtagtc cgcgagttcc ttcatgagcg gccgcgccac
    21781 ctggcgcacg ttcatggagg cgagcagcgg atagccgatg gagagcaccg ccgagccgag
    21841 ctgatacttg ccgaggcgca tgttgtggcg caggtatccg agcttggtga gcgtgtacgt
    21901 aagccgggaa acggtaggct tgggtaagcc ggtgcgcacc gaaatttcct tgttgccgag
    21961 caacggttcc agcggcgtga agcaacgtaa aacttccagg ccacgtgcca acgtcgtcgc
    22021 gaactggcgg tcgccttcat gctcgtagct gaatgtcgcc gggttcgggg aaatagcggc
    22081 cgtggcttcg ggtgcgccca tttagatgct gtctcctggt ctttcggttg agttctgggg
    22141 tcggtggaaa cgcgcggaac gctgcgcgtt tcgctattct gtccgcccac gcgggcgtgt
    22201 caacttagtc atccgagatt tcacatagcg aaatatgcgc tccctctttg atctggcggg
    22261 aaaaatcgcc ctcgtgaccg gtgcaacgcg cggcatcgga cgatcgatcg cggaagagct
    22321 tggcaaagcg ggcgcgaagg tcgcgatctg cagccgcaag gccgatgcct gcgcagaaac
    22381 cgaagccgac ctggcgcgaa gcggcaccga ggtgctcgcc atgccctgca acgtgtcgcg
    22441 caaggaagag ttacaggcgc tcgtcgacgc aacacgcgcc cgctgggggc ggatcgacat
    22501 cgtggtcgcc aacgccgcga ccaatcccta ttacggtccg ctcaccggca tcggcgacga
    22561 agccttcgac aagattttcg ccaacaacct gaagagcgtg ctgtggctcg ccgggatgac
    22621 gttgcccggc atgaccgccg gcggaagctt catcctggtc ggctcgatcg gcgggatcat
    22681 tgccaatacg gtgatcgggg cttacggcat gtcgaaggcg gcgggacatc acctggtgcg
    22741 caacctcgcg gcggaatggg ggccgaagaa cgtgcgcgtg aacgcgatcg cccctgggct
    22801 catcaagacc gagttcgctc gcgcgctgtg ggaggacccc aagcgcgccg aagaacgcgt
    22861 ttcaaacaca ccactgcggc gccttggaga gccgcgcgac atcggcggca tcgctgtttt
    22921 tcttgcgtcg cctgctgccg cgtttatcac cggacaatgc atcgttgccg acggcggcgt
    22981 tactattctc tagccatgga cctcaattac tcccctgaag agctggcgtt ccgcgacgag
    23041 gtccggtcct ggctggccgc gaaccttccg caggagctga aggacaaggc gcactactcg
    23101 aaggaagagc tgatgcgctg gcaccgcatc ctcgcggcca aaggctgggt cgcgcccgcc
    23161 tggccgaagg aatggggcgg cacgggctgg agcgcggtgc agcgctacat ctacgaggaa
    23221 gagctcggct acgccggcac gccgccgatc attccgttcg gcatcaatat gtgtgcgccg
    23281 gtcctcatcg cgttcggcac cgacgcccag aaaaagcgct tcctgccgcg tatctataac
    23341 ggcgaagatt tctggtgcca gggctattcg gagccagggt cgggaagcga tctcgcttcg
    23401 ctgaaaacca aagcggtgaa ggcgggcgcc cactacgtgg tgaacgggca gaagatctgg
    23461 accacgctcg cgcactacgc cgattggatc ttctgcctgg tgcgcaccga cccgacggtg
    23521 gagaagcgcc aggaaggcat ctcctttctg ctcatcgaca tgaagagccc gggcatcacg
    23581 ctgcagccgc tcatgctgat ggacggggcg catcacgtga acgaagtttt cttcgacgat
    23641 gtcaaggtgc cggccgacaa cctggttcat aaggagaacc agggctggac cgtggccaag
    23701 tatcttctcg gctacgagcg catgaacacc gggcgcatcg gcgaatcgaa gcgcgagttg
    23761 cgcaagctga aagagagatt cccggagaag atgaaggatg tgcgctggcg cgaccggctt
    23821 tcgcgcgtgg aagtcgagct gatggcgctc gagatcacga acctgcgctt cctcgaccgc
    23881 atgcgccgca ccgggcagcc gccgggcgcg gacgtttgca tgctgaagat caagggaacc
    23941 gagatccagc aggcgctgag cgaactcgcc atgcaggcgg ccgacccggc ggcgcgggag
    24001 acgcaggcgc tcgagcggcg ctacgccgac atgcgcaaga ccacgatcta tgccgggtcg
    24061 aacgagatcc agcgcaacat catcgccaag gcgacgctgg ggttatgaac ttcgattaca
    24121 ac
//
LOCUS       DASTPD010000019         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1027213, whole genome shotgun sequence.
ACCESSION   DASTPD010000019 DASTPD010000000
VERSION     DASTPD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027213"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFR57_00840"
     CDS             complement(<1..523)
                     /locus_tag="VFR57_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU0257953.1"
                     /translation="MARPERAALALMDTNGFDCYSPQQIAERVEAACLAKATTPLLSL
                     AMLGVLAGAYIGLGSMFYVIVASDATLGFAASRVLGGVVFSLGLVLVVVAGAELFTGN
                     NLLAMAWAHRALTTKDVLQNWAVVCAANFVGAGGLAAFVFLSGLADRLAGTYVEIAQA
                     KAALPFWTAFFRGV"
     gene            complement(510..746)
                     /locus_tag="VFR57_00845"
     CDS             complement(510..746)
                     /locus_tag="VFR57_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257954.1"
                     /translation="MIKAIWILWPSFLAGAAGAALLFAILDPVDLRFVGPLELGRKAG
                     YTLAFFFFWALAAASSWLTCFLQARAGREPDGAP"
     gene            complement(743..1261)
                     /locus_tag="VFR57_00850"
     CDS             complement(743..1261)
                     /locus_tag="VFR57_00850"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEU0257955.1"
                     /translation="MDSPAVHRRRLLRPAHEPLGHRFWELHAVTPWYRERWPWLLILG
                     PAAVLVAGAITTWIAFASADGLVAEDYYKQGLGINRLLAREDTGRRLGVSADLEIEQG
                     AVTVKMAGAAPEALFVHLAHATRAGHDVRLRLTPRADGRYAAELPPMPAGRWRAVIED
                     PRGTWRIVKEAR"
     gene            complement(1174..1497)
                     /locus_tag="VFR57_00855"
     CDS             complement(1174..1497)
                     /locus_tag="VFR57_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257956.1"
                     /translation="MWTKTAIAVALALLAGGCAMSSNWHLMDSGYAIDTENGSDFAIE
                     VHLNQLKEFGGDVNSSAFRHFVAERLKIHDLCPKGWTVLPCIEDGSCVQHTSRSVTVF
                     GSCTP"
     gene            complement(1553..2872)
                     /gene="ccoG"
                     /locus_tag="VFR57_00860"
     CDS             complement(1553..2872)
                     /gene="ccoG"
                     /locus_tag="VFR57_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEU0257957.1"
                     /translation="MNAADAPLPQEVSLYEVRRKIYPRAVHGWFAAWRWILVWATQIV
                     FYGGPWLEWNGRQAVLFDIAERKFFLFGLVFWPQDVIYLAVLLIVSALSLFLFTAVAG
                     RLWCGYACPQTVYTEIFLWIERVIEGDRNARMRLDRERTGLRKLGLKSAKHAVWLAVA
                     LWTGITFVGYFTPIRDLLSTAPGAWESFWALFYGFATYGNAGWMREQVCKYMCPYARF
                     QSAMFDKDTLIITYDAKRGEPRGKGQGKGDCVDCGICVQVCPTGIDIRNGLQYECIGC
                     AACIDGCDRVMEKVGRPKKLIRYSKGRVLRPRVLVYAAILGAISAAMAVSLYMRVPLK
                     VDVIRDRAAIAREVEGGLIENVYRLQIMNTAEQARAFDINVSGLPGILVWGENITGLP
                     AASSRMVPVRVRAEAAFPPGSHPIQFTVRALGVAGVEVREEAAFIVR"
     gene            complement(2869..3762)
                     /gene="ccoP"
                     /locus_tag="VFR57_00865"
     CDS             complement(2869..3762)
                     /gene="ccoP"
                     /locus_tag="VFR57_00865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEU0257958.1"
                     /translation="MSEFFSGFWDVYVAVLTVVSIFACALFLKSQSIQRVSGAAETTG
                     HKWDEDLAEYNNPLPRWWSWLFYITILFSLAYLVLYPGLGSWKGTLGWSQVGQLKEEQ
                     ELAEKQYGPVYKKFASTEVDALLKLPEALAIGQKLFLNNCAQCHASDGAGSRGFPNLT
                     AGAFQWGRDATTVKTTIAEGRTGMMPPFGEALGAEGVKNVAQYVRSLSGLTADSIRVE
                     LGKEIFAKTCVACHGAEGKGNAALGAPNLTDKNWLHGSAEAVVIETIAKGRNSQMPAH
                     KHVLDEARIHLLTAYVLSLSK"
     gene            complement(3759..3905)
                     /locus_tag="VFR57_00870"
     CDS             complement(3759..3905)
                     /locus_tag="VFR57_00870"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEU0257959.1"
                     /translation="MNMDINLIRTLVTVAALGAFLAIVVWAYLPHRREKLDAQAQSIL
                     REDR"
     gene            complement(3902..4516)
                     /gene="ccoO"
                     /locus_tag="VFR57_00875"
     CDS             complement(3902..4516)
                     /gene="ccoO"
                     /locus_tag="VFR57_00875"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEU0257960.1"
                     /translation="MKLTHDLIERNQALLIVLVIAVVAVGGLVEIVPLSFQKSVTEPV
                     AGLKPYTPLQLTGRDIYIREGCYNCHSQMVRPFRAEVERYGPYSVAGEFVYDRPFQWG
                     SKRTGPDLHRVGGRYSDEWHRLHLLNPRDLVPESNMPAYPWLAKARANAEDIEAKMKA
                     LKTLGVPYTDAEIAGARKELDGKTEMDALIAYLQGLGTAVKRGK"
     gene            complement(4513..>5357)
                     /locus_tag="VFR57_00880"
     CDS             complement(4513..>5357)
                     /locus_tag="VFR57_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU0257961.1"
                     /translation="AGAQDAMVQWWYGHNAVGFFLTAGFLGMMYYFVPKQAGRPIYSY
                     RLSVVHFWALIFTYRWAGPPHLHYTALPEWTQSLGMVFSLILLAPSWGGMINGIMTLS
                     GAWHKLRDDPILKFLVISLSFYGMSTFEGPMMSIKTVNALSHYTDWTIGHVHSGALGW
                     VGLISMGSLYYLIPRLFGRTEMHSVRAINLHLWVATIGIVLYIAAMWIAGVMQGLMWR
                     AVSDDGSLTYTFVESVKATYPFYAIRLLGGVLYLSGMLIMAWNVFKTVTIAKPVEAMI
                     PQPA"
BASE COUNT          990 a         1745 c         1758 g          864 t
ORIGIN      
        1 gcaccccgcg gaagaacgcg gtccagaaag gcagcgcggc cttggcttgc gcgatctcga
       61 cgtaggtccc ggcgagccga tcggcaagcc cggagaggaa tacgaacgcc gcgagcccgc
      121 cggcgccgac gaagttggcg gcgcagacga ccgcccagtt ctgcaggacg tccttcgttg
      181 tcagggcgcg atgcgcccac gccatggcga gcagattatt gccggtgaag agctcggcgc
      241 cggccacgac gaccagcacg agaccgagcg agaacaccac cccgccgagc acgcgcgacg
      301 cggcgaagcc gagcgtggcg tcgctcgcca cgatcacata gaacatcgat ccgaggccga
      361 tgtaggcgcc ggcgagcacg ccgagcatgg cgagcgagag aagcggagtg gtggccttcg
      421 cgagacaggc cgcctcgacg cgctcggcaa tctgctgcgg cgaatagcag tcgaacccgt
      481 tcgtatccat gagcgcgagc gctgcgcgct cagggcgcgc catccggttc gcggcctgcg
      541 cgcgcctgca ggaaacacgt gagccagctc gacgccgcgg cgagcgccca gaagaaaaag
      601 aaggcgagcg tgtagcccgc cttgcgaccg agctcgagcg ggccgacgaa gcgcagatcc
      661 acggggtcga ggatcgcgaa aagcagcgcc gcgccggccg cccccgcgag gaacgagggc
      721 cacaaaatcc agatcgcctt gatcaccgcg cttccttgac gatacgccag gtgccgcgcg
      781 ggtcctcgat gaccgcgcgc cagcgtccgg ccggcatggg cggcagttcg gcggcgtagc
      841 ggccgtcggc gcgcggcgtg aggcgcagcc gcacgtcgtg gccggcgcgc gtcgcatgcg
      901 cgagatggac gaagagcgcc tcgggcgcgg cgccggccat cttcacggtc acggcaccct
      961 gctcgatctc gagatctgcg gatactccga gtcggcgccc ggtatcttca cgcgcgagca
     1021 gacgattgat cccgagtccc tgcttgtaat agtcctcggc caccaggccg tcggccgatg
     1081 cgaaagcaat ccacgttgtg atcgctccgg cgaccagcac ggcagccggt cctagtatca
     1141 ggagccaagg ccagcgttcg cgataccagg gcgtcacggc gtgcagctcc caaaaacggt
     1201 gaccgagcgg ctcgtgtgct ggacgcagga gccgtcttcg atgcacggca ggactgtcca
     1261 ccccttcggg caaagatcgt ggattttcag gcgctcggcg acgaaatggc ggaacgcgga
     1321 gctattcacg tcaccgccga actccttcag ttggttgagg tggacttcga tcgcgaaatc
     1381 actgccgttc tcggtgtcga tcgcgtagcc cgagtccatc aggtgccagt tgctgctcat
     1441 cgcgcaaccc ccggcgagga gcgcgagcgc cacggcgatc gctgttttgg tccacataag
     1501 cccatcttag gtcagcggtg atccccacat ttgacacaag tcaatcccgc gcctagcgca
     1561 cgatgaaggc ggcttcttcc cgcacttcga ccccggcgac gccgagcgca cgcaccgtga
     1621 actggatcgg gtggctgccc ggagggaagg ccgcttccgc acgcacacgc accggcacca
     1681 tgcgcgacga agcggcaggc agcccggtga tgttttctcc ccagacgaga attccgggca
     1741 ggccgctcac gttgatatcg aatgcgcgcg cctgctcggc ggtattcatg atctgcaggc
     1801 ggtacacgtt ctcgatcagc ccgccctcca cttcgcgcgc gatcgctgcg cgatcgcgga
     1861 tcacgtccac cttgagcggc acgcgcatgt agaggctgac cgccatcgct gcgctgatgg
     1921 cgccgaggat cgccgcgtac acaagcacgc gtggccgtag gacgcggccc ttcgaatagc
     1981 ggatgagctt cttcggtcga cccacctttt ccatgacacg gtcgcagccg tcgatgcacg
     2041 ccgcgcagcc tatgcactca tactggagac cgtttcggat atcgatgccg gtcgggcaga
     2101 cctgcacgca gatgccacag tccacgcagt cgcctttacc ttgccctttg ccgcgcggct
     2161 cgccgcgttt cgcgtcgtag gtgatgatga gcgtgtcctt gtcgaacatc gcgctctgga
     2221 agcgcgcgta cgggcacatg tacttgcaga cctgctcgcg catccaaccg gcgttgccat
     2281 aagtggcgaa gccatagaac aatgcccaga agctctccca ggcgccgggg gcggtgctca
     2341 atagatcgcg gatcggcgtg aaatagccga cgaaggtgat tcccgtccag agcgcaaccg
     2401 cgagccagac ggcatgcttc gcgcttttca agccgagctt gcgcaaaccc gtcctttccc
     2461 tgtcgaggcg catgcgcgcg ttacggtcgc cctcgatgac gcgttcgatc cacagaaaga
     2521 tctcggtgta gacggtctgc ggacacgcgt agccgcacca caggcgcccg gcgacggcgg
     2581 tgaagaggaa gagggatagc gcgctcacga taaggagcac cgcgaggtaa atgacgtcct
     2641 gcggccagaa caccagcccg aacagaaaga acttgcgctc ggcgatgtcg aacagcaccg
     2701 cctggcggcc gttccactcg agccaggggc cgccgtagaa cacgatctgc gtcgcccaga
     2761 ccaggatcca gcgccacgcg gcgaaccacc cgtgcaccgc gcgcgggtag atcttgcgcc
     2821 ggacctcgta gagcgagact tcctgaggaa ggggcgcgtc cgcggcgttc acttcgaaag
     2881 cgaaaggacg taggcggtga gcagatggat gcgcgcctcg tccagcacgt gcttgtgcgc
     2941 cggcatctgg ctgttgcggc ccttggcgat ggtctcaatc accaccgcct cggccgagcc
     3001 gtgcagccag ttcttgtcgg tgaggttcgg cgcgccgagc gcggcgttgc ccttgccctc
     3061 ggcgccgtgg caagcaacgc aggtcttcgc gaagatctcc ttgccaagct ccacgcggat
     3121 cgagtccgcg gtaagacccg acaacgagcg cacgtactgc gcgacgttct tcaccccttc
     3181 ggcgccgagt gcttcgccga acggcggcat catcccggta cggccctcgg cgatggtcgt
     3241 cttgaccgtc gttgcgtcgc ggccccactg gaaggcgccc gccgtgaggt tgggaaagcc
     3301 gcgcgagccg gccccgtcgg aagcatggca ctgcgcgcag ttattcagga aaagcttttg
     3361 cccgatggcg agcgcctcgg gaagcttgag cagtgcatcg acttccgttg acgcgaactt
     3421 cttgtacacc gggccgtact gcttctcggc aagctcctgc tcctccttga gctggccgac
     3481 ctggctccag ccgagcgtcc ccttccagct gccgagcccc ggatagagca cgaggtaagc
     3541 gagcgagaat aggatcgtga tgtagaaaag ccagctccac cagcgcggca gcgggttgtt
     3601 gtactcggcg aggtcctcgt cccacttgtg ccccgtggtc tccgcggccc cgctcacacg
     3661 ctggatgctc tggctcttga ggaaaagcgc gcacgcaaag atcgatacca ccgtcagcac
     3721 ggcgacgtac acgtcccaga agccgctgaa gaattcgctc accggtcctc ccgcaggatg
     3781 ctttgcgcct gcgcgtccag tttctcgcgg cgatgcggca ggtaggccca caccacgatg
     3841 gcgaggaacg cgccgagcgc ggccaccgtg acgagggtgc ggatgagatt gatgtccatg
     3901 ttcatttgcc tcgcttcact gccgtgccca agccctggag gtaggcgatg agcgcatcca
     3961 tctcggtctt gccgtcaagc tccttgcgag cgccggcgat ctccgcgtcg gtgtacggca
     4021 cgccgagcgt cttgagcgcc ttcatcttgg cttcgatgtc ttcggcgttc gcgcgcgcct
     4081 tcgcgagcca cggatacgcg ggcatgttgg attcaggcac gaggtcgcgc gggttgagga
     4141 gatgcaggcg gtgccactcg tcggaatagc ggccgccgac gcggtgcagg tcgggcccgg
     4201 tgcgcttgga gccccactgg aaggggcggt cgtacacgaa ctcgccggcc accgaatacg
     4261 gcccgtagcg ctccacctcg gcgcggaaag gccgcaccat ctgcgaatgg cagttgtagc
     4321 agccttcgcg gatgtagatg tcgcgcccgg tgagctgcag cggcgtgtag ggcttgagcc
     4381 ccgcgaccgg ctcggtcacc gacttctgga acgaaagcgg cacgatttcg accaggccgc
     4441 cgaccgcgac caccgcgatc acgaggacga tgagcagcgc ctggttgcgc tcgatgaggt
     4501 cgtgggtgag cttcatgcgg gttggggaat catcgcttcg accggtttgg caatggttac
     4561 cgtcttgaac acgttccagg ccatgatgag catgccggag agatagagca caccgccgag
     4621 caggcggatc gcgtaaaagg gataggtcgc cttcacgctc tcgacgaagg tgtaggtaag
     4681 gctgccgtcg tcgctcaccg cgcgccacat caggccctgc atcacgccgg cgatccacat
     4741 cgcggcgatg tagaggacga tgccgatggt ggccacccag aggtgcaggt tgatcgcgcg
     4801 cacgctgtgc atctcggtgc gcccgaagag ccgcgggatc aggtaataga gcgagcccat
     4861 cgagatcagg ccgacccagc cgagcgcgcc ggagtgcacg tgcccgatgg tccagtcggt
     4921 gtagtgcgag agcgcgttca ctgttttgat cgacatcatc ggcccctcga aggtcgacat
     4981 gccgtagaac gagagcgaga tgacgaggaa cttgaggatc gggtcgtcgc gcagcttatg
     5041 ccacgcgccc gagagggtca tgatgccgtt gatcatgccg ccccaggacg gcgcgagcag
     5101 gatgagcgag aacaccatgc cgagcgactg cgtccactcc ggaagcgcgg tgtagtgcag
     5161 gtggggcgga ccggcccacc tataggtgaa gatgagcgcc cagaagtgca ccaccgagag
     5221 gcggtacgaa tagatcgggc gcccggcctg cttcgggacg aagtaataca tcatcccgag
     5281 gaaaccggcc gtgaggaaga atcccaccgc gttgtggccg taccaccact gcaccatcgc
     5341 gtcctgcgcg ccggcgt
//
LOCUS       DASTPD010000020         6151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_8057, whole genome shotgun sequence.
ACCESSION   DASTPD010000020 DASTPD010000000
VERSION     DASTPD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8057"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFR57_00885"
     CDS             <1..549
                     /locus_tag="VFR57_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEU0257962.1"
                     /translation="PRSLSEADVEALLAAPNPATPLGLRDRAMLETLYATGLRVSELV
                     ALKVFEVNLDANVVRVLGKGSKERLVPLGEEAADWISRYLKTRDARSDALFVTGRASG
                     MTRQAFWHLIRRYGARAIPGKKLSPHVLRHAFATHLINHGADLRVVQLLLGHADISTT
                     QIYTHVARERLKALHAKHHPRG"
     gene            546..1010
                     /locus_tag="VFR57_00890"
     CDS             546..1010
                     /locus_tag="VFR57_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU0257963.1"
                     /translation="MKTPATEYLSLRNILFSEHLYGYVEHGGTEVAARALNVPEHHIV
                     KTLVMEDQARKPLIVLMHGDRKVSTKNLARQVQRESIEPCKPQAAQRHTGYLVGGTSP
                     FGTRKPLPVFAERSIRSLGEIYINGGRRGLLLKIRPADLETALPVTWVDVAL"
     gene            complement(985..1341)
                     /locus_tag="VFR57_00895"
     CDS             complement(985..1341)
                     /locus_tag="VFR57_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU0257964.1"
                     /translation="MDAIVFRDLRVEALVGIHRRERHVLQTVSIDLEIGVPGAAVFVS
                     DKVADTIDYEQVALRIRALAASGHFRLVETFADRIATLLVNEFRAPWAKVSVAKVGIL
                     ANAKYVGVTIERRRPT"
     gene            1373..1984
                     /gene="plsY"
                     /locus_tag="VFR57_00900"
     CDS             1373..1984
                     /gene="plsY"
                     /locus_tag="VFR57_00900"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0257965.1"
                     /translation="MGTAAVLLGAYLIGSISFAIVVSRVMRLPDPRSYGSKNPGATNV
                     LRTGRKAAAVLTLAGDAAKGWVAVWLAFVFAAEAAFGGLAAFLGHLFPVFHRFQGGKG
                     VATAAGVLFGLDWRLGLGTLVTWLVIAATLRYASLASIVAAVFAPFATALILDWKSAF
                     IVGAMALLLIWRHKTNVGRLLGGIEPRFGENKPNSADPSAASR"
     gene            complement(1950..2924)
                     /gene="tsaD"
                     /locus_tag="VFR57_00905"
     CDS             complement(1950..2924)
                     /gene="tsaD"
                     /locus_tag="VFR57_00905"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0257966.1"
                     /translation="MLILGIETSCDDTGVALYDSAAHKVVAHAVHTQASMHEVYGGVV
                     PELASRDHIRRLVPLTRKVLGGKTIDGIGYTEGPGLAGALLVGASFAHGLAVSLGVPA
                     VGVHHLEGHLLSPLLSARRPEFPFLALLVSGGHTQLMRVDGVGRYELLGDTQDDAAGE
                     AFDKTAQLLGLGYPGGPALSKLAESGTPERYRLPRPMIGAGLDFSFSGLKTAVLTLVK
                     NERPEPADVARAFVDAIVEVLVVKCARAVESTGLTQLVVAGGVGANAQLRSALDAEAA
                     RGGFDVFYPEPELCTDNGAMIALAAAYRLRNATGSHAFNVKPRWDLQS"
     gene            2971..3183
                     /gene="rpsU"
                     /locus_tag="VFR57_00910"
     CDS             2971..3183
                     /gene="rpsU"
                     /locus_tag="VFR57_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU0257967.1"
                     /translation="MPTVRVKENEPFEVAMRRFKRTVEKTGLLTELRAREYYEKPTAE
                     RKRKLAAAVKRHHKRLRSQMLPQKLY"
     gene            3180..3626
                     /locus_tag="VFR57_00915"
     CDS             3180..3626
                     /locus_tag="VFR57_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU0257968.1"
                     /translation="MSLKSKITEDMKAAMRAKDSARLSAVRLLLSGIKQREVDERKEL
                     SDVEVTAVIEKMIKQRRESIAQYEKAARKDLVDAEKFELDLLIGYLPQQMSDAELSQA
                     VDAAVSDAKPAGIKDMGKVMALLKARLAGKADMGKVSALVKTRLSG"
     gene            3637..5388
                     /gene="dnaG"
                     /locus_tag="VFR57_00920"
     CDS             3637..5388
                     /gene="dnaG"
                     /locus_tag="VFR57_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0257969.1"
                     /translation="MIPDSFKQDLLNRVDVVDVVQRYVQLRKAGANYVGLCPFHNEKT
                     PSFSVSPTKQFYHCFGCGAHGNAVGFLMAYAAMGYVDAVKELAAQVGMQVPESRPRTP
                     EEAARKEREPDLYAVMEKAMNFYRGELKNSPRAIEYLKARGLTGEIAARYRIGYAPDD
                     WQALRGVFPNYEDQTLAEVGLLVQNDEGKRYDRFRDRIMFPILNPRGAVIGFGGRIVD
                     KGEPKYLNSPETALFEKGRELYGLPQARDAIRAASRVVVVEGYMDVVALAQFGVGYVV
                     ATLGTATTPVHVSKLLRLADELVFCFDGDAAGRKAAWRALEVSLPLAPDHKPVRFLFL
                     PQGEDPDSYVRKYGFEAFEARVREAETLSQFLLSQLRSEADLDTPEGRARLISVSKSH
                     IQKITAPALRLQLTNEIAQLARVAEADIGALLELPRRPAFTRASPRLHHFDAPISLEW
                     SLLTALLSDLTLVEHVDPARLAPDRAETQALLAIRRQCETSEEELSFPLLMDALSGNS
                     CIETVLRAQRYGEDLAFDVDAARSEFQHALTQIDLRQRKMELDNLRERLSSREDLVAF
                     NEKNLAYKRLQGALPSA"
     gene            5455..>6151
                     /locus_tag="VFR57_00925"
     CDS             5455..>6151
                     /locus_tag="VFR57_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEU0257970.1"
                     /translation="MVQKRKKLRKVRAAKKKAKVVKRLKAKIRAARKPIAAKKPALKA
                     RKVPALKPAPTKADDKAAKAAKTPIKSAKQALELLEAKVNEVKGKGRRGRGGRRGGKD
                     AWLIPAELPKEDAETRRTRLKNLIKLGKERGFLTYSEINDHLPDDLVDAEQIEAIIST
                     FGDMGIQVYDQAPDQETLLIASDTQTVAQPDEDVEEQAEAALSTVDSEFGRTTDPVRM
                     YMREMGSVELLTRE"
BASE COUNT         1155 a         1969 c         1989 g         1038 t
ORIGIN      
        1 ccgaggtcgc tgtccgaagc cgacgtggaa gcgctgctcg ccgcgcccaa ccctgccacg
       61 ccgctcggcc tgcgcgaccg cgcgatgctt gagacgctct acgcgacagg cctgcgcgtg
      121 tcggagcttg tcgcgctcaa ggtcttcgag gtgaacctcg acgcgaacgt ggtgcgcgtc
      181 ctcggcaagg gctccaagga gcgccttgtg ccactcgggg aagaggcggc agactggatt
      241 tcacggtacc tgaagacgcg cgacgcaaga tccgatgccc ttttcgtcac cggccgcgcc
      301 agcgggatga cacggcaggc gttctggcac ctgatccgcc gctatggggc gcgcgcgatc
      361 ccgggaaaga agctttcgcc gcacgtgctg cgccacgcgt tcgcgaccca cctcatcaac
      421 cacggcgccg atctgcgcgt cgttcagttg ctcctcggac acgccgacat ctccaccacg
      481 cagatctata cgcacgtggc gcgcgagcgc ttgaaggctt tgcatgccaa gcaccacccg
      541 cgtggatgaa gacgccagcg accgagtatc tctccttacg aaatattctt ttttcggagc
      601 acctgtacgg gtatgtcgag cacggcggta ccgaagtggc cgcgcgcgct ttgaacgtgc
      661 cggagcatca catcgtcaag acgctcgtca tggaggatca ggcgcgcaag ccgctcatag
      721 tgctgatgca cggtgaccga aaggtatcga ccaagaacct tgcacgccag gtgcagcgcg
      781 agtcaatcga gccgtgcaag ccgcaggcgg cgcagcgcca cacgggctac ctcgtgggtg
      841 gcacgtcgcc cttcggcact cgcaagccgc tgcccgtgtt cgccgagcgc tcgattcgca
      901 gcctcggcga gatctacatc aatggtggcc gccgcggcct gctcctcaag atccgccccg
      961 cagatcttga gaccgcgctc cccgtcacgt gggtcgacgt cgctctatag tgacgccgac
     1021 gtacttggcg ttggcgagta tcccgacctt ggcgacgctc acctttgccc acggcgcccg
     1081 gaactcgttg accaggagcg tcgctatgcg gtcggcgaac gtctcgacca agcggaagtg
     1141 cccgctcgcg gcgagagcgc ggattctgag cgctacctgt tcatagtcga tcgtgtcggc
     1201 caccttgtcg ctgacgaaga ccgcggcgcc ggggacgccg atctcgagat cgatcgacac
     1261 cgtctgcagc acgtgccgct cgcgccggtg gatgccgacg agcgcctcga cgcgcaggtc
     1321 gcggaagacg atggcgtcca tgatccggac cgattctata gaatcgccag cgatgggaac
     1381 cgcggcagtc ctgctgggtg cctacctgat tggctcgatt tcctttgcca tcgtcgtgag
     1441 ccgcgtcatg cggctcccgg acccgcgcag ctacggctcg aagaatcccg gcgccacaaa
     1501 cgtgttgcgc accgggagga aggcagcggc ggtgcttacg ctcgccggcg acgccgccaa
     1561 gggctgggta gccgtatggc tcgcatttgt ctttgccgcc gaggctgcgt tcggcgggct
     1621 cgccgccttc ctcggccatc tcttcccggt gttccaccgt ttccagggcg gcaagggcgt
     1681 cgcgactgcg gcaggcgtgc tgttcggcct ggattggcgt cttgggcttg gaacgctcgt
     1741 cacgtggctc gtgatcgccg ccacgctccg ctacgcgtcg cttgcatcga tcgtggcggc
     1801 cgtcttcgcg ccctttgcga ccgcccttat cctcgattgg aaaagcgcct tcatcgtcgg
     1861 cgccatggcg ctgctcctta tctggcgcca caagaccaat gtgggccgcc tgcttggcgg
     1921 catcgagcct cgcttcggcg aaaataaacc taactctgca gatcccagcg cggcttcacg
     1981 ttgaaagcgt gcgagccggt tgcgttcctt agcctgtaag ccgctgcgag cgcgatcatc
     2041 gcgccgttgt cagtgcaaag ctcgggttcc gggtagaaga cgtcgaagcc gccgcgcgca
     2101 gcttccgcgt cgagcgccga gcgcagctgc gcgtttgctc ccactccgcc cgcaaccacg
     2161 agctgcgtga gcccggtgct ttccaccgcg cgcgcacact tgaccaccag cacttccacg
     2221 atagcgtcca cgaaagcacg cgcaacgtcc gccggctcgg gccgttcgtt cttcaccagc
     2281 gtcagcacgg cggtcttaag gcccgagaag ctgaagtcaa ggccggcgcc gatcattggg
     2341 cgcggcaagc ggtagcgttc cggcgtgccg ctctcggcga gcttcgaaag cgccggcccg
     2401 ccggggtacc ccagccccag aagctgtgcg gttttatcga aggcctcgcc ggccgcgtcg
     2461 tcctgggtgt cgccgaggag ctcgtagcga ccgacgccat ccacgcgcat gagctgggta
     2521 tggccgcccg agacgagcag cgccaagaag ggaaactctg gacgtcgtgc ggacagaagc
     2581 ggcgaaagca ggtgaccttc caggtggtgg acgcctacgg cgggcacccc caaggagacc
     2641 gcaaggccat gcgcgaaaga agcgcccact aacagcgctc ctgccagccc gggaccttcg
     2701 gtgtagccga ttccgtcaat ggtttttcct ccgaggactt ttctcgtcag cggtacgagc
     2761 ctgcgaatgt ggtcgcgcga ggcgagctca ggtacgaccc ctccatagac ctcgtgcatc
     2821 gaggcttgtg tatgcaccgc gtgcgcgacg accttgtggg cggcgctgtc gtagagcgcg
     2881 acacccgtgt cgtcgcagga ggtctcgatg ccgaggatga gcatgagacg attctgatat
     2941 acaattcagc cccttttcca gcaggggttt atgccgacag tacgcgtcaa ggaaaacgag
     3001 cctttcgaag tggcgatgcg ccgcttcaag cgcaccgtgg agaagaccgg actccttacc
     3061 gagctgcgtg cccgcgagta ttacgaaaag cccaccgccg aacgcaagcg caagcttgcc
     3121 gcggctgtga agcggcacca caagcgtctg cgcagccaga tgcttccgca gaagctgtac
     3181 tgagtcttaa gtcgaaaatc actgaagaca tgaaggccgc gatgcgcgcc aaggacagtg
     3241 cgcgcctgtc cgcggtccgc ctgctgttgt ccggaatcaa gcagcgggaa gtcgacgagc
     3301 gcaaggagct gtccgacgtc gaggtcactg cggtgatcga gaagatgatc aagcagcgtc
     3361 gcgagtcgat tgcacagtac gaaaaagcgg cgcgcaagga cctggtggac gcagagaagt
     3421 tcgagctgga cctactcata ggctacctgc ctcagcaaat gagcgatgcc gagctctccc
     3481 aggctgtgga tgccgcagtt tccgacgcaa aaccggccgg tattaaggac atgggcaagg
     3541 taatggcgct gctcaaggcg cgtcttgctg gcaaggcaga catgggcaag gtatccgccc
     3601 tagtcaagac caggctttcg ggataaaaag cggggcgtga tccccgactc gtttaagcag
     3661 gacctgctca atcgggtcga tgtcgtggat gtggtgcaac gctacgtcca gctgcgcaag
     3721 gcgggggcaa actacgtcgg cttgtgcccg ttccacaacg aaaaaacccc ttccttcagc
     3781 gtgagcccga cgaagcagtt ctaccactgc tttggttgcg gcgcgcacgg taacgcggtc
     3841 ggattcctga tggcgtacgc cgccatgggc tatgtcgacg ctgtgaagga gctcgccgca
     3901 caggtcggca tgcaggtccc cgaatcccga ccgcgcacgc ccgaagaggc ggcgcgcaaa
     3961 gagcgcgagc ccgacctcta cgcagtcatg gaaaaggcga tgaatttcta tcgcggcgag
     4021 ctgaagaact cgccgcgcgc gattgagtac ttgaaagcac gcggcctgac gggtgagatc
     4081 gctgcccgtt accgcatcgg gtacgcgcct gatgattggc aggcactcag gggcgtattc
     4141 cccaactacg aagaccagac gctcgccgag gtcggcttgt tggtgcagaa cgacgaaggc
     4201 aagcgctacg accgcttccg cgatcgcatc atgtttccca tcctcaaccc gcgcggcgcg
     4261 gtgatcggct tcggcggccg gatcgtggac aagggagagc cgaagtacct gaactcgccg
     4321 gagacggccc ttttcgagaa ggggcgcgag ctctacgggc tgccgcaggc gcgcgatgcg
     4381 attcgagcgg cgagccgggt ggtggtggtt gaggggtaca tggacgtcgt ggcgctcgcc
     4441 cagttcggcg tcggctacgt cgtcgccacg ttgggcaccg ccacgacgcc ggtccacgtg
     4501 tcgaagctgc tgcggctcgc agatgagctt gtgttctgct tcgacggcga tgccgccggg
     4561 cgcaaggcgg cctggcgggc actggaggta agcctgccgc tcgcccccga ccacaagccg
     4621 gtccggttcc tgttcctgcc gcagggcgag gatccggata gctatgtgcg gaagtacggg
     4681 ttcgaggcgt tcgaggcgcg cgtacgcgaa gccgagaccc tgtcgcaatt cctgctttcg
     4741 cagctgcgtt cagaggccga tctcgacacg cccgaggggc gcgcccggct gatttccgtc
     4801 tccaagtcgc atatccagaa aatcacagcg ccggcgctgc gcctgcagct tacgaacgag
     4861 atcgcccagc tcgcccgggt cgccgaagcc gacatcggag cgctgcttga gctcccgcgt
     4921 cgtccggcct tcacgcgggc ttcgccgcgt ctccatcatt tcgatgcgcc gatctcactc
     4981 gaatggagct tgctcaccgc gctgctcagc gatctcacgc tcgtagagca cgtcgacccc
     5041 gctcggcttg cccccgaccg cgcggaaacc caggcgttgc tcgcgatccg gcggcagtgc
     5101 gaaacctcgg aggaggagct gagcttccct ttgctgatgg acgcgctcag cgggaattcc
     5161 tgcattgaaa cggtgttgag agctcagaga tacggggaag acctcgcatt cgacgttgac
     5221 gcggcgcgca gcgagtttca gcatgcactg acgcagattg atctccggca gcggaagatg
     5281 gaacttgaca acctgcgcga gcggctgagc tcccgggaag acctcgtagc gttcaacgag
     5341 aagaacctgg cttacaagag gctccaaggg gcacttccaa gcgcgtgacc ggtcacatat
     5401 aatgcgcggt tcgatttttt gcgggtattc aggtagttat aaggcggggg acggatggtc
     5461 cagaagcgga agaagctgag gaaagttcgg gcggccaaga agaaggccaa ggtcgtcaag
     5521 cgccttaagg ccaagatccg cgcagcgcgc aagccgatcg ctgcgaagaa gcctgccctc
     5581 aaagcacgca aggtcccggc gctgaaaccg gcgccgacca aagccgacga caaggcggcc
     5641 aaggcggcca agactccgat caagagcgcc aagcaggcgc tggagctcct cgaagcgaag
     5701 gtcaacgagg tcaagggcaa aggccggcgc gggcgcggcg gccgccgcgg cgggaaggac
     5761 gcgtggctga tcccggccga gctgccgaag gaagatgcgg agacgcggcg cacgcgcctg
     5821 aagaacctga tcaagctcgg caaggagcgc ggcttcctca cgtactcgga gatcaacgac
     5881 cacctgcccg acgatctggt cgacgccgag cagatcgaag cgatcatctc gacgttcggc
     5941 gacatgggca tccaggtcta cgaccaggcg ccggaccagg aaaccctgct catcgcctcc
     6001 gacacccaga cggtggcgca gcccgacgag gacgtcgagg agcaggccga ggccgcgctc
     6061 tccaccgtcg attccgagtt cggccgcacc accgacccgg tgcgcatgta catgcgcgag
     6121 atgggctcgg tggagctgct cacgcgcgag g
//
LOCUS       DASTPD010000021        21363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_8219, whole genome shotgun sequence.
ACCESSION   DASTPD010000021 DASTPD010000000
VERSION     DASTPD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8219"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFR57_00930"
     CDS             <1..599
                     /locus_tag="VFR57_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU0257971.1"
                     /translation="VVLAAGNYAYVRGLGKATDETWYVYRKGVPLVDPDTNKTLGYEA
                     VYLGTAQLTRPGEPATVVLTSAVQEVEAGDKLVAAGRPQPVNYAPHAPSTKIRGRVIA
                     IYGGVAQVGEAGPQSVISINRGKADGIEVGHVLALYNRGGTVRDVTKRRGAADAQIQL
                     PDERAGLAFVFRVFDRISYALVMQVTRPVSPLDVVQTP"
     gene            612..1706
                     /gene="dprA"
                     /locus_tag="VFR57_00935"
     CDS             612..1706
                     /gene="dprA"
                     /locus_tag="VFR57_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0257972.1"
                     /translation="MNPDPGLASWLQLALTPGLGPVSVQRLLQQFGLPQNALARNRRE
                     LAAHVPPAALAALDSDPVALAVKRSLAWAEQPGHSIVTLADEGYPHSLLEIPDPPPLL
                     FARGRLELLARPALAIVGSRNATQQGEANAESFAKALSDAGLAIVSGLALGIDAAAHR
                     GGLAGAGSTIAVLGTGIDVLYPARNAGLAEHIAQQGLILSEFALGTAPAAHNFPRRNR
                     LISGLARGCLVVEAALASGSLITARAAADQGRDVFAIPGSIHSPLAKGCHALIKAGAK
                     LVESAEDVLTELNGFRPIAFASTQQVPSTETGLLAHMGHDPVDVDLLCSRAGLSPDQV
                     SSELLRLELDGRVAALPGGLYQRLEKAPRR"
     gene            1745..2203
                     /locus_tag="VFR57_00940"
     CDS             1745..2203
                     /locus_tag="VFR57_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEU0257973.1"
                     /translation="MYEILVFLFEHCQQAEIAYDRDRVAKKLSAAGFEDSDISEALRW
                     LADMAHARHCVREPLPDARTSFRAYAPRELAKLDAQCRGFLMTLEHSGILSADTRELV
                     IERALAAAGDTLSLEQLKLVVLMVLWNQQTPTSQLVAEDLFTAPQARLAS"
     gene            2282..4801
                     /locus_tag="VFR57_00945"
     CDS             2282..4801
                     /locus_tag="VFR57_00945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEU0257974.1"
                     /translation="MAKKLIIAEKPSVAADIARALGGFKRSGDYFESDDYVLSSAVGH
                     LLELRVPEEHDVKRGKWSFARLPVIPPEFTLAPIEKSETRLNLLLRLMKRKDVGALVN
                     ACDAGREGELIFRYIVQHARNKKPIERLWLQSMTPQSIRDGFSKLRPDKELLPLADAA
                     KSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAILVEREERIRAFTARA
                     YWEVQARFAAKAGEYPGRWFDPKFKKDEDTERKAERLWSAAEAETIVAACRGKSGIAS
                     EETKPSTQLSPLLFDLTSLQREANARFGFPARMTLSLAQALYERHKVLTYPRTDARAL
                     PEDYLGTVGNTLEELSQTRDFAPFAKQILKQGWVKPNKRVFDNSKISDHFAIIPTLQT
                     PRNLNEAEQKLYDLVVRRFLAVFYPAAEYLQTTRITRVGEHHFRTEGKVLQTPGWLAV
                     YGRAVGEESENLPSITQDEKVAVREITGNANETKPPPRYTEATLLSAMEGAGKFVEDD
                     ELREAMAAKGLGTPATRAAIIEGLIREEYVHREARELVPTPKAFSLMFALHMLHIVEL
                     GSPELTGEWEHKLKLIEAGKMTREEFMAQIRGLVKNVVSTIKHGEIPDVAYATVPAPC
                     PKCGGAVQENYRKVQCQKCDFSLWRVISGREWAPEELGELFSKRFVGPLNGFRSKQGR
                     PFSAGIRLTDELRLEFDFGQGAGGEGDEPVDFSAQEPLGACPKCSARVFEHGVAYVCE
                     KAVGAQRSCDFRSGRMILQQPIEREQMKKLLESGRTDLLTGFVSKKGRKFKAFLVKQP
                     DGKIGFEFQAKAPKDKAEKPAQKQAPAAEKTTRPGSARRAR"
     gene            complement(4767..7169)
                     /gene="gyrB"
                     /locus_tag="VFR57_00950"
     CDS             complement(4767..7169)
                     /gene="gyrB"
                     /locus_tag="VFR57_00950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU0257975.1"
                     /translation="MSDAEKEYGSDAIKVLKGLEAVRKRPGMYIGDTSDGTGLHHMVF
                     EVVDNAVDEALAGFCDEIVVTVHTDNSVSVLDNGRGIPTGIKDDDELKRSAAEIVMTE
                     LHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTVWREGTVHQMEFRNGVALAPLR
                     PTGGTERRGTEVHFLASRETFGNIDYHYDILARRLRELSFLNNGVRIVLVDQRSGKEE
                     NFAFSGGVKGFVEYMNRAKNVLHPNVFLGHGVKEGITVEVAMQWNDSYQENVLCFTNN
                     IPQKDGGTHLTGLRAAMTRVLNKYIEEHEIAKKAKVETTGDDMREGLACVLSVKVPEP
                     KFSSQTKEKLVSSEVRPVVEELVAERLREFLDERPHDARTITVKIVEAAAAREAARKA
                     REMTRRKGVLDSMGLPGKLADCQERDPAKSELYLVEGDSAGGSAKQGRDRKFQAILPL
                     KGKILNVERARFEKLLSSAEIATLITALGCGIGTEEYNPDKLRYHRIIVMTDADVDGS
                     HIRTLLLTFFYRQMRELVERGHIYIAQPPLYRAKLGKEERYLKDEHELNAFMLKQALV
                     DAALLPGAAGEPISGDTLAELAKSYLLAEAVMERVSRLVDREVLRAMLSGVQVDLSRR
                     DTAEASAEALEAAIGGQGFAVAPHFDQKTERYQVRVERTRHGNVRMSTIDTEFIHSGD
                     YAQIRQTAEMLRGLIGPGASVQRGDKRQPVRDFAEAMKWLLGEVEKAMTLQRYKGLGE
                     MNPAQLWETTMDPATRRLLKVQIEDGIAADEIFTKLMGDEVEPRRAFIESNALGVRNL
                     DV"
     gene            complement(7171..8277)
                     /gene="dnaN"
                     /locus_tag="VFR57_00955"
     CDS             complement(7171..8277)
                     /gene="dnaN"
                     /locus_tag="VFR57_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU0257976.1"
                     /translation="MNLAKAKRDELLGPLSAVSGIIERRHTLPILSNVLVERSGEALS
                     FLATDIEIQITARSGVLAAAEGRPVTVGARKLLDILRALPDGAEVALQHQDKRLVVRA
                     GKSRFTLQTLAAEDFPRLAKPAGESARFELEQKALRRVLALVQYAMAQQDIRYYLNGL
                     LMVIEDGMLKLVATDGHRLAYAALKLGAQLPRQEVIIPRKTVLELGKLLADSDASVKV
                     EVGATQAAFSFGTVDLVSKLIDGKFPDYTRVIPTSHKNRLQAAREPLRQALLRAAILS
                     NEKFRGVRWVLADGSLKIVSSNAEQEEAHEELEVSYSGDALDIGFNVNYLLDVLNNVS
                     GESIECAFGDAASSALISYPSERDFKYVVMPMRI"
     gene            complement(8469..9803)
                     /gene="dnaA"
                     /locus_tag="VFR57_00960"
     CDS             complement(8469..9803)
                     /gene="dnaA"
                     /locus_tag="VFR57_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU0257977.1"
                     /translation="MQNLWDACLRRLEQEIPAQQFNTWIRPLAPEAGDASDTLVLTAP
                     NRFILELVRERFAARIERLAAETSGRQLGLRLVLARVAEVAPVRAQAPTSPRGVDAPT
                     VQERTRLNPAFTFDSFVTGKANQLARAAALQVAENPGVSYNPLFVYGGVGLGKTHLVQ
                     AIGNQLADHKPQARIRYTHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFS
                     NKGRTQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVAIEPPEL
                     EMRVAIVLKKAEADSVELSEDIAFFIAKHLRSNVRELEGALKKVLAFARFSGRELSLE
                     LAKEALQDILNVASRQVSVEGIQKTVAEYFKIKISDMHSKKRSRNVARPRQVAMALAK
                     DLTQMSLPEIGEAFGNRDHTTVLHACRTIASLRKHDTALNRDYVVLEQVLKG"
     gene            9921..10061
                     /gene="rpmH"
                     /locus_tag="VFR57_00965"
     CDS             9921..10061
                     /gene="rpmH"
                     /locus_tag="VFR57_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU0257978.1"
                     /translation="MATKRTYQPSKTRRARRHGFLVRSRSAGGRKVLRSRRARGRRRL
                     GL"
     gene            10103..10408
                     /gene="rnpA"
                     /locus_tag="VFR57_00970"
     CDS             10103..10408
                     /gene="rnpA"
                     /locus_tag="VFR57_00970"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU0257979.1"
                     /translation="MRRKDEFENLLRHGSRRSLNGYTFYVARRESGEARLGLLVSRRH
                     AARAVVRNGIKRCIREAFRLTQGDLGALDVLVRPPYGAQPSAQMVAQLRALLPRLKP"
     gene            10405..10617
                     /gene="yidD"
                     /locus_tag="VFR57_00975"
     CDS             10405..10617
                     /gene="yidD"
                     /locus_tag="VFR57_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0257980.1"
                     /translation="MTVRVLRFALKTYRWCVRPLLAPCCRFFPSCSEFAEEALVRHGF
                     WRGCWLTARRLCRCGPWHPGGHDPVP"
     gene            10623..12272
                     /gene="yidC"
                     /locus_tag="VFR57_00980"
     CDS             10623..12272
                     /gene="yidC"
                     /locus_tag="VFR57_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0257981.1"
                     /translation="MDTQRLILLVIFSFSLLMLWEAWEKEKRPKPAPSPATAQGVPVP
                     EKPVAAAAPAAKPSAAMPEGASPAKGEIVRISTDLIAVEVDTLGGTLKRVELLRHKDS
                     KDLSRSLVLLGPEHHYEAQSGLAGEGGPNHRTFWRAEPGERALAAGRDEVTLVLSATG
                     KDGAQVRKTYTFKRDSYVIDVALEVQNAGSAPLGAYAYFQLTHDGKPETNTNTVAETF
                     GAQSFIGFAVYTDERKFEKVYPSDLDKGRSDHVKQATNGWLAFVQHYFVSAWLPPQGV
                     PRDYVTEKRPDGIYAARAMVPLNVAAGSSAAVKVPIYVGPQEQRRLQAAAPGLDLVVD
                     YGWLAIIAWPLFWLLEKFHALSGNWGVAIILLTVLIKIVFFPLSAASYKSMAKMKLIT
                     PRLTKLREMYAHDRQKMNQAMMELYKTEKINPLGGCFPILVQIPVFIALYWVLLAAIE
                     LRHAPFMLWIKDLSALDPYYVLPILMTATMVLQTRMNPVPPDPVQARVMQIMPFVFSI
                     FFFFFPAGLVLYWLVNNILSIAQQWQIQRMFDRDKPAHAKR"
     gene            12259..13578
                     /gene="mnmE"
                     /locus_tag="VFR57_00985"
     CDS             12259..13578
                     /gene="mnmE"
                     /locus_tag="VFR57_00985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU0257982.1"
                     /translation="MPSDELTRDTIAAVATPAGRGGIGVLRVSGSLALAIAKKVVGRA
                     PKPRTALHAVFRDAGGEAIDQGIALYFPAPHSYTGEAVLELQGHGGPVVMQVLLAACL
                     DAGARLAEPGEFTRRAFLEGKLDLAQAEAVADIIDAASREAARSALRSLSGEFSAAIG
                     ALQSGLVELRALTEAMLDFPEEDVDTLDREDAVRRLDALRRGLDGILAQSRQGSLLRS
                     GIHIVIAGRPNVGKSSLLNRLAGEERAIVAAVPGTTRDALREPIEIDGVPLVVVDTAG
                     LRISSDAVECMGIERTERELARADAAIVVLDATGGAEETPPIPPDLARIEVYNKIDLV
                     PGFAAPRGAIALSAKTGEGMDALRAAILRVAGWSSSGEPVYLARERHLRALDEARGHL
                     ELAAKEQRWELFAEELRLAHRALGSITGQFDVEHLLGEIFARFCIGK"
     gene            13724..15559
                     /locus_tag="VFR57_00990"
     CDS             13724..15559
                     /locus_tag="VFR57_00990"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEU0257983.1"
                     /translation="MNAPSTPLDQSQAPSWVRHRRLRQWVAEVARMAKPETIVWCDGS
                     QAEYDRLCGELVAAGTFIRLNDRLRPNSYLARSHPSDVARMEDRTFICSQKREDAGPT
                     NNWIDPREMRATLTRLFDGCMRGRTMYVMPFSMGPIGSPIAQVGVEISDSAYVVANMR
                     IMTRMGRAVAEWLGEDRPFVPCVHSVGAPLAPGRKDVAWPCSEKEKWIVHFPETREIW
                     SFGSGYGGNALLGKKCLALRIASVMARDEGWLAEHMLILGVQAPSGEKTYMAAAFPSA
                     CGKTNLAMLIPPAGFSGWKVTTIGEDIAWIKPGPDGRFRAINPEYGLFGVAPGTSQHT
                     NPNAMRMLQANVIFTNVAMTPEGDVWWEGMTDEPPARLIDWQGKDWTPDSGRLAAHAN
                     ARFTVPAAQCPSMDPAWEDPAGVPISAFIFGGRRSTTVPLVVEAPSWEEGTYMAATLG
                     SETTAAITGKVGVVRRDPMAMLAFCGYNMGDYFAHWLKMGRAVKQPPKIFRVNWFRRD
                     AGGKFLWPGFSENMRVLAWIVERCQGSARGQETPLGLMPRYEDLNWSGLEKVSATRFS
                     ELTQVDAAAWKEELRSHDELFAKLGARLPAALEARRGRMHEKLAA"
     gene            complement(15548..16723)
                     /locus_tag="VFR57_00995"
     CDS             complement(15548..16723)
                     /locus_tag="VFR57_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257984.1"
                     /translation="MLSLARYRTAFSSAPLRRTFLISIIGRLPIGVTGLAILVLVQSA
                     TGSFARGGFAAGCYVSGLALAAPGLGRMIDRHGPRAVLRFCALAFPSALAFLIYGSEA
                     AVPWLEIAAAAAAGASFPPITVCMRTYMRRALTDEALLSAAFSAESVLIEVIFIVGPL
                     VVAVFAATAAPALAVWFSAGCALVGTLVFLRSPALQGWRIERRSATGLLGPLARRDFA
                     HLVAIVLCFATAFGFLEIGITAYAVETSDAALAGVLLGLMSAGSALGGLAYGSRAWHA
                     PLARQFAAALALTAGGLAVLAAPWQPWVFAVWSLLAGVAIAPALIIQSMLVTKSAATE
                     HSTEAFTWTTSALLAGVGIGLAFGGMLLERLPWSAAFVAGAAAALLSAAGARVFLRR"
     gene            16811..18649
                     /gene="mnmG"
                     /locus_tag="VFR57_01000"
     CDS             16811..18649
                     /gene="mnmG"
                     /locus_tag="VFR57_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU0257985.1"
                     /translation="MVFPREFDVIVVGGGHAGTEAALAAARLGCATLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVRELDALDGVMALATDEAGIQFRTLNASKGPAVRATRAQADRV
                     LYRNAIRRRLENQPNLWLFQQQVDDILLEGARAVGVATQVGVAFRSRTVVLTTGTFLS
                     GLIHVGLQHYQAGRAGDPPALRLAARLRELRLPVGRLKTGTPPRLDARSIDFSLMEEQ
                     RSDSPEPVFSFLGRREIHPAQRPCWIAHTNERTHEIIRAALDRSPMFTGAIQGVGPRY
                     CPSIEDKIHRFVGKASHQIFLEPEGLTTHEIYPNGISTSLPFDVQLELVRSIRGLENA
                     HVVRPGYAIEYDYYDPRHLRTSLETKSISGLFFAGQINGTTGYEEAAAQGLLAGLNAG
                     LQTLGRDAWCPKRDEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLMLREDNADLRLTE
                     TGRRLGLVADERWMAFARKRDAIAQETARLKGVFVSAGPQEKRPAYELLRRPEVRHAD
                     LPQDGVVDPMVAEQIEIQAKYEGYIERQRDEVARRGEFEDRALPSELDYRDVRGLSAE
                     VQQKLNWHRPETIGQAARIAGITPAAISLLLVHLKRGFGKPEKKRA"
     gene            18646..19266
                     /gene="rsmG"
                     /locus_tag="VFR57_01005"
     CDS             18646..19266
                     /gene="rsmG"
                     /locus_tag="VFR57_01005"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU0257986.1"
                     /translation="MTPGAALDQGLAQLGLSLPTSAPEVLLKYLALLSKWNRTYNLTA
                     IRDPLAMVAHHLLDSLAVLPYLPLGDHARVADAGSGAGLPGIPIAIARPSWHVALAES
                     SQKKAAFLRQATIELALANVEVHEGRVERWQPRPLFDLVISRAFAALGDFIAACRHLV
                     QPAGVLAAMKGAAPEDAPRECQLVRLDVPLLGAQRHLVLCPASSQS"
     gene            19242..20003
                     /locus_tag="VFR57_01010"
     CDS             19242..20003
                     /locus_tag="VFR57_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0257987.1"
                     /translation="MPRVLAVVNQKGGVGKTTTTVNLAAALAQAGRRVLLVDLDPQGN
                     ATMGSGVDKRAAVRTVYHALLGLGELAGIRTRAERGGYDVVPANRDLAGAEVELVELP
                     ARETRLKAALERVADDYDYILIDCPPSLSLLTVNALAAAQRVLIPMQCEYYALEGLSD
                     LVGTIKRVRANLNPTLEIAGLLRTMYDPRNTLSQQVSRELESHFGDKVFRTLVPRNVR
                     LAEAPSYGVPAVVWDASSKGAQAYVALASEVLESP"
     gene            20000..20830
                     /locus_tag="VFR57_01015"
     CDS             20000..20830
                     /locus_tag="VFR57_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0257988.1"
                     /translation="MKPRGLGRGLDALLGGDEQPRDALLTLPVGRIRPGRYQPRTKMD
                     QQALAELAASIRSQGLMQPLLVRPVDRDRYELVAGERRWRAAQMAGLEEVPALVREIP
                     DEAALAMSLIENIQRENLNPMEEAAGIQRLVDEFRMTHEQAADAVGRSRSATTNLLRL
                     LKLARPVQEMLMEGALEMGHARALLALDGARQIESAKRVAAKGLSVRATEALVHTMLR
                     GPQARTKRRDRDLARLEEETSERLGTTVEIRAGKKGSGRIVLHYSSLEHLEELLAKLK
                     "
     gene            20917..21285
                     /locus_tag="VFR57_01020"
     CDS             20917..21285
                     /locus_tag="VFR57_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU0257989.1"
                     /translation="MRLPLSKPIRIVLKWQLVATAVATLAAALVAGQHAAASAAAGGA
                     VSIVAGMAAATIAALGRAKSAGSVLASALRAEAVKLGLALVLLWLVLVNYPQAAVGVL
                     LATFVVTMLIFSMAFFVREY"
BASE COUNT         3767 a         6946 c         6933 g         3717 t
ORIGIN      
        1 gcgtggtgct cgcggcgggc aattacgctt acgtgcgcgg cctcggcaag gccacggatg
       61 agacctggta tgtctaccgc aagggcgtac cgctcgtgga tcccgatacg aacaagactt
      121 tgggctacga ggcggtctat ctcggcacgg cgcagttgac gcgtcccggc gagccggcga
      181 cggtggtgtt gacgagcgcg gtgcaggaag tggaagccgg cgacaagctt gtcgccgcgg
      241 gaaggccgca accggtgaat tacgcgcccc acgcaccgag caccaagatc cgcggtcgcg
      301 taatcgcgat ctacggtggc gtggcgcagg tcggtgaagc gggtccgcag tcggtaatca
      361 gcatcaaccg cggcaaagcc gacggcatcg aggtcggaca cgtgctcgcg ctgtacaacc
      421 gtggcggcac ggtccgtgac gtgaccaagc ggcgcggagc cgctgatgcg caaattcagc
      481 tgcccgacga gcgcgcggga ctcgcgtttg tgtttcgcgt gttcgaccgg atatcctatg
      541 cgctcgtcat gcaggtcacg aggccggtct ctccgctcga tgtggtgcag acaccgtaag
      601 aagaaagtgc agtgaaccca gacccggggc tcgcaagctg gctgcagctg gccctcaccc
      661 cgggtctcgg accagtcagc gtccaacggc tgctccagca gttcggcctt ccgcagaacg
      721 cgctcgccag gaatcgccgc gagctcgccg cgcatgtgcc gccggccgcg ctcgccgcgc
      781 tcgattcgga cccggtcgcg ctggcggtga agcgatccct cgcttgggct gagcaaccgg
      841 gacactcgat cgtcacgctc gccgacgaag gctacccaca cagcttgctc gagattccag
      901 acccgccgcc gcttctattc gcgcgcggcc gcctggaact tctcgcacgg cccgcgctcg
      961 ccatcgtggg cagtcgaaac gcgacgcagc agggcgaagc gaacgccgag tcgtttgcca
     1021 aggccctgag cgacgcgggg ctcgccatcg tctcgggcct cgcgctcggc atcgacgccg
     1081 cagcgcatcg cggcgggctc gccggcgccg gatcgaccat tgcggtgctc gggaccggca
     1141 tcgatgtcct ctatccggcc cgcaatgccg gacttgcaga acacatcgct cagcaagggc
     1201 ttatcctcag cgagtttgcg ctcggcacgg cgcccgcggc gcataatttc ccgcgccgca
     1261 accgcctgat cagcggactc gcgcgcggct gcctcgtcgt ggaagcggcg ctcgcttccg
     1321 gctcgctcat caccgcgcgc gcggcggctg atcagggccg cgatgtgttc gcgattcccg
     1381 ggtcgatcca ttcgccgctc gcgaaaggtt gccacgcgct catcaaggct ggagcgaaac
     1441 tcgtggaatc ggcagaggac gtgctcacgg aactgaacgg ctttcgtccg attgccttcg
     1501 catcgacgca gcaggtaccc agcaccgaga ccggcctgct cgcgcacatg gggcatgatc
     1561 ccgtggacgt agacttgctg tgctcgcgcg ccgggctgtc gcccgatcag gtgtcgtccg
     1621 aattgctgcg tctggagctc gacgggcgcg tcgcggcgct gcccggcggg ctgtatcaac
     1681 ggctggaaaa ggcaccccgc cggtaaaccc aatccccatc gtcgcgttat aagaagggag
     1741 cgccatgtac gaaatcctcg ttttcctgtt cgagcactgc cagcaagcgg aaatcgccta
     1801 cgaccgcgat cgcgtggcga aaaagctgtc cgcggcgggc tttgaggatt ccgacatcag
     1861 cgaggccttg cgctggctgg cggacatggc gcacgcgcgc cactgtgtgc gcgagccgct
     1921 gcccgacgcg cgcacgagct ttcgcgccta cgcgccgcgc gagctcgcga agctcgacgc
     1981 acagtgccga ggtttcctga tgacgctcga gcattccgga attctgagcg ccgacacgcg
     2041 cgagctcgtc atcgagcgcg cgctcgccgc cgcgggcgat acgctgagcc tcgagcagct
     2101 caagctcgtc gtgctcatgg tgctctggaa ccagcagacg cccaccagcc agctcgtcgc
     2161 cgaggacctt ttcacggcac cgcaggcgcg gctcgcaagc tagcgcattt tttcgcaccg
     2221 tccgcaacgt ggttgaacca cgcgcggatt tgcaattatc atctgcggcc tttcgcgccg
     2281 catggccaaa aagctgatca tcgccgagaa accgtcggtc gcggccgaca ttgcgcgcgc
     2341 gctgggtgga tttaagcgca gcggcgacta tttcgagagc gacgactacg tgctctccag
     2401 cgcggtcggc catcttcttg agctgcgcgt ccccgaagag cacgacgtca agcgcggcaa
     2461 atggtctttc gcccggctgc cggtaatccc gccggaattt acgctcgcgc cgatcgagaa
     2521 gtcggaaacc cgcctcaatc tgctcctgcg cctcatgaag cgcaaggacg tcggcgcgct
     2581 ggtgaacgcc tgcgacgcag gccgcgaggg cgagctcatc ttccgctaca tcgtgcagca
     2641 cgcgcgcaac aagaagccga tcgagcgcct gtggctgcag tcgatgacgc cgcagtcgat
     2701 tcgcgacggg ttctccaagc tgcgtcccga caaggagctc ctcccgctcg ccgacgcggc
     2761 gaaaagccgc tccgaagccg actggctcgt cggcatcaat ggtacgcgcg cgatgaccgc
     2821 gttcaactcc aaggaaggcg gcttctatct caccacggtg ggccgcgtgc aaacgcctac
     2881 gcttgccatt ctggtcgagc gcgaggaacg catccgcgcg tttacggcgc gtgcttactg
     2941 ggaggtgcag gcgcgcttcg cggccaaagc gggcgagtat ccgggacgct ggttcgatcc
     3001 gaaattcaag aaggacgagg acactgagcg caaggcggaa cggctctgga gcgcggccga
     3061 ggccgaaacc atcgtcgccg cctgccgcgg caagagcggc attgcctcgg aggaaaccaa
     3121 gccgtcgacg cagctttcgc cgctgctctt cgatctcacg agcctgcagc gtgaggccaa
     3181 cgcgcgcttc ggtttcccgg cgcgcatgac gctgtcgctc gcgcaggcgt tgtacgagcg
     3241 ccacaaggta ctgacctatc cgcgcaccga cgcgcgcgca ctgcccgagg attatctcgg
     3301 cacggtaggg aacacgctgg aggagctttc gcaaacgcgc gacttcgcgc cgttcgccaa
     3361 gcaaatcctg aagcagggct gggtgaaacc gaacaagcgc gtgttcgaca acagcaagat
     3421 ctcggaccac ttcgccatca ttccgacgtt gcagacgccg cgcaacctga acgaagccga
     3481 gcagaagctt tatgacctcg tggtacgccg cttcctagcg gtgttttatc cggcggcgga
     3541 gtacctgcag accacgcgca tcacgcgcgt cggcgagcat catttccgca cggaaggcaa
     3601 ggtgctgcaa acacccggct ggctcgcggt ctacggtcgc gcggtcggcg aggaaagcga
     3661 gaacctcccg tccatcaccc aggatgaaaa ggtcgcggtg cgcgaaatca ctggcaacgc
     3721 caacgagacc aagccgccgc cgcgctacac cgaggcgacc ttgctttcgg cgatggaggg
     3781 cgcgggcaaa tttgtcgagg acgatgagct gcgcgaagca atggccgcca aggggcttgg
     3841 caccccggcg acgcgcgccg cgatcatcga aggcctcatc cgggaggagt acgtacaccg
     3901 cgaggcgcgc gagcttgtgc cgacgccgaa ggccttctcg ctcatgttcg cgctgcacat
     3961 gctgcacatt gtcgagctcg gttcgccgga gctcaccggc gagtgggagc acaagctcaa
     4021 gctgatcgag gccggcaaga tgacgcgcga ggaattcatg gcgcaaattc gcggcctggt
     4081 gaagaacgta gtgagcacca tcaagcacgg cgagattccc gacgtcgcct acgccacggt
     4141 gccggcgcca tgcccgaagt gcggcggcgc ggtgcaggag aactaccgta aggtgcagtg
     4201 tcagaaatgc gacttttcgc tctggcgcgt gatttccggg cgggaatggg cgcccgagga
     4261 acttggcgag ctattcagca agcgcttcgt cggccccttg aacggcttcc gcagcaagca
     4321 agggcggccc ttcagcgcgg gcatccggct caccgacgaa ttgcgcctcg agttcgactt
     4381 cggccagggc gcgggcggcg agggcgacga gccggtcgat ttttcagcac aggagccact
     4441 cggcgcgtgc ccgaagtgca gcgcgcgcgt cttcgagcat ggcgtcgcct acgtgtgcga
     4501 aaaagccgtc ggtgcccagc gcagctgtga tttccgctcc ggacgcatga tcctgcagca
     4561 gccgatcgag cgcgagcaga tgaagaagct gctcgaaagc gggcgcaccg atctcctgac
     4621 cggctttgtc tcaaagaagg gccgcaagtt caaagccttc ctcgtcaagc agcccgacgg
     4681 caaaatcggc ttcgaattcc aggcgaaagc accgaaggac aaggccgaga agccggccca
     4741 gaagcaggcg cccgcggcgg aaaagactac acgtccaggt tccgcacgcc gagcgcgtta
     4801 gattcgatga acgcgcggcg cggctcgacc tcgtcgccca tgagtttcgt gaagatctcg
     4861 tcggcggcga tgccgtcttc gatctgcacc ttgagaaggc gccgcgtcgc gggatccatg
     4921 gtggtctccc agagctgcgc ggggttcatt tctccgagac ctttgtaacg ctgcagcgtc
     4981 atggcctttt ccacctcgcc gagcagccac ttcatcgcct cggcgaaatc gcgcaccggc
     5041 tggcgcttgt cgccgcgctg cacgctcgcg cccggaccaa tcagcccgcg cagcatctcg
     5101 gcggtctgcc ggatctgcgc gtaatccccg gagtggatga actcggtgtc gatcgtgctc
     5161 atgcgcacgt tgccgtggcg cgtgcgctcg acgcgcacct ggtagcgctc ggttttctga
     5221 tcgaagtgcg gcgccaccgc gaagccttgg ccgccgatcg ccgcctcgag cgcctcggcg
     5281 gacgcctcgg cggtgtcgcg gcgcgaaagg tcgacctgca ccccgctcag catagcgcgc
     5341 agcacctcgc ggtcgaccag gcgcgagacg cgctccatta ccgcttccgc gaggaggtat
     5401 gactttgcga gctcggccag ggtgtcgccg ctaatcggct cgcccgcggc gccaggaagg
     5461 agcgcggcgt cgacaagcgc ctgcttcagc atgaacgcat ttagctcgtg ctcgtccttc
     5521 aggtaacgct cttccttccc gagctttgct ctgtagagcg gcggctgggc aatgtagatg
     5581 tgaccgcgct cgaccagctc gcgcatctgt cgataaaaga acgtgagcag cagggtgcgg
     5641 atatgcgagc cgtccacgtc ggcgtcggtc atcacgatga tgcggtggta gcgcagcttg
     5701 tcggggttgt actcttcagt gccaatgccg cagccgagcg ccgtgatgag cgtcgcgatc
     5761 tccgccgagg agagcagctt ctcgaagcgg gcgcgctcca cattcaagat tttccccttg
     5821 agcggcagaa tcgcctggaa cttgcggtcg cgtccttgct tggctgaacc gccggccgag
     5881 tcgccctcga cgagatagag ctccgacttc gccgggtcgc gttcctggca gtcggccagc
     5941 ttgcccggca gccccatcga gtcaagcacg cccttgcggc gcgtcatctc gcgcgctttg
     6001 cgcgccgcct cgcgcgctgc cgcggcttcg acgatcttca cggtgatggt tcgcgcgtcg
     6061 tgcggccgct cgtcgagaaa ctcgcgcagc cgctcggcga cgagctcctc gaccaccggc
     6121 cgcacttccg aggaaaccag cttttccttc gtctgcgacg agaacttggg ctccggcact
     6181 ttcaccgaca gcacgcaggc gaggccttcg cgcatgtcgt cgcccgtggt ctcgaccttc
     6241 gccttcttgg cgatctcgtg ctcctcgatg tacttgttca gaacgcgcgt catcgccgcg
     6301 cgcaatcccg tgagatgcgt gccgccgtcc ttctgcggaa tattgttggt gaagcacagc
     6361 acgttctcct ggtacgaatc gttccactgc atcgcgactt cgacggtgat gccctccttg
     6421 acgccgtggc cgaggaaaac gttcgggtgc aacacgttct tcgcgcgatt catgtactcg
     6481 acgaagcctt tcactccgcc cgagaacgcg aagttttcct ccttacccga gcgctgatcg
     6541 acgagcacga tcctcacgcc attgttcaag aacgagagct cgcgcaggcg ccgcgccagg
     6601 atgtcgtagt ggtagtcgat gttgccgaat gtctccctcg aggcgaggaa gtgcacctcg
     6661 gtgccgcgcc gctcggtgcc cccggttggc cgcagcggcg cgagcgccac gccgttgcgg
     6721 aactccatct ggtggactgt gccctcgcgc cagacggtga ggcgcagcca ttcggaaagt
     6781 gcgttcacca ccgatacgcc tacgccatgc agcccgccgg aaaccttgta gctgttcgag
     6841 tcgaatttgc cgccggcgtg cagctccgtc atcacgattt ccgcggccga gcgcttgagt
     6901 tcgtcgtcat ccttgatgcc ggtcggaatg ccgcgcccgt tgtcgaggac cgaaaccgag
     6961 ttgtcggtgt gcacggtgac gacgatctcg tcgcaaaagc ccgcgagcgc ctcgtcgacg
     7021 gcgttatcga ccacctcgaa caccatgtgg tgcagacccg taccgtcgga ggtgtcgccg
     7081 atgtacatcc cggggcgctt gcgcaccgcc tccaggccct tcaggacctt gatcgcgtct
     7141 gaaccgtatt ccttttcggc gtcgctcaat tcagatcctc atcggcatga caacgtattt
     7201 gaagtccctt tcggagggat agctaatgag cgcgctcgac gccgcgtcgc cgaaagcaca
     7261 ttcgatgctc tcgccggata cgttgttgag gacgtccagc aggtagttca cgttgaagcc
     7321 gatgtcgagc gcatcgcccg agtagctcac ctcgagctcc tcgtgcgctt cttcctgctc
     7381 ggcgttcgaa gagacgatct tcaggctgcc gtcggcgagc acccaacgca cgccgcggaa
     7441 tttttcgttg gaaaggatcg ccgcgcgcaa cagcgcctgg cgcagcggct cgcgcgccgc
     7501 ctgcaggcgg ttcttgtggc tcgtgggaat tacgcgtgtg taatccggaa acttgccgtc
     7561 gatcagcttg gagacgaggt cgacggtgcc gaagctgaac gcggcctggg tggcgccgac
     7621 ttcaaccttg accgacgcat cgctatcggc gagaagcttg ccgagctcca gcacggtctt
     7681 gcgcggaatg atgacttcct ggcgcggcag ctgcgcgccg agcttgagcg ccgcgtaagc
     7741 cagccgatga ccgtcggtgg cgacaagctt cagcatgccg tcctcgatca ccatcagcag
     7801 gccgttcaga tagtaccgga tgtcctgctg agccatcgcg tactgcacaa gcgcaagcac
     7861 ccggcgcagg gccttctgct cgagttcgaa gcgcgccgac tcgccagcgg gcttggccag
     7921 gcgcggaaaa tcctcggccg ccagggtctg caacgtgaag cggcttttgc ccgccctgac
     7981 cacaaggcgc ttgtcctgat gctggagcgc cacttccgcg ccgtcgggaa gcgcacgcag
     8041 gatatcaagg agcttgcggg cacccacggt gaccgggcgc ccttcggcgg ccgccaacac
     8101 gccgctgcgc gcggtgatct ggatttcgat gtcggtggcg aggaaggaaa gagcctcgcc
     8161 gctgcgctcc acgagcacgt tggagagaat gggcagcgtg tgacggcgct cgatgatccc
     8221 gctcactgca gagagcggcc cgagcaactc gtcccgcttt gcttttgcaa gattcatttt
     8281 tatttacctt agtagtagct aatagagcgt gcgttttgcc gggataactg cgcaaccgcc
     8341 tgaggcgtaa cgcattacgc tcgctcgcac cttgaggaaa tgctgtggac ggcgcgcgag
     8401 gctttgtgga tcgtttcggg ctttcgcccg ccccggcgcg ttattcacaa tcgatcccca
     8461 gcttctcttc agcccttcag cacctgctcg agcacgacgt aatcgcggtt gagcgcggta
     8521 tcgtgcttgc gcaggctcgc gattgtgcgg caggcgtgca gcacggtagt gtggtcgcga
     8581 ttgccgaacg cctccccgat ctccggaagg ctcatctgcg tgaggtcctt ggcgagcgcc
     8641 atcgccacct ggcgcggccg ggctacgttg cggctgcgct tcttggaatg catgtcagag
     8701 atcttgatct tgaagtactc ggccacggtc ttctggattc cctcgacgct cacctggcgg
     8761 ctcgcaacgt tcaggatgtc ctgcagcgcc tccttggcaa gctcaagcga caactcgcgg
     8821 ccggagaaac gcgcgaacgc cagcaccttc ttgagcgcgc cttcgagctc gcgcacgttc
     8881 gagcgcaggt gcttggcgat gaagaaggcg atgtcttccg acagctcgac cgagtcggcc
     8941 tcggccttct tcaggacgat ggcgacgcgc atctcgagct ccggcggctc gatcgccacg
     9001 gtcagtcccc acccgaagcg cgagatcagc cgctcctcca tgcctgcgat ctccttcgga
     9061 taggtatcgc aggtgatcac aatttgtttg tgcgcctcga ccagggcatt gaaggcatag
     9121 aagaattcct cctgggtgcg gcccttgttc gagaagaact ggatatcgtc gatcaggagc
     9181 agatcgagcg aatggtagta acgcttgaat tcgtcgaaag acttctgctg ataggcgcgc
     9241 accacatcgg tcacgtactg ctccgcgtgg gtgtagcgaa tgcgcgcctg cggcttatgg
     9301 tcggcaagct ggttgccgat cgcctgcacc aggtgcgttt tgccgaggcc gacgccgccg
     9361 tagacgaaaa gcgggttgta cgaaactcca ggattctcgg cgacctgcag cgcggcggcg
     9421 cgtgcgagct ggttcgcctt gccggtcacg aagctgtcga aagtgaaagc cggattgagg
     9481 cgcgtgcgct cctgtacggt cggcgcgtcg acgccccgcg gcgacgtagg cgcctgcgcg
     9541 cgcacaggcg cgacctcggc gacgcgcgcg agcaccagcc gcaggccgag ctggcgcccg
     9601 ctcgtttcgg cggcgaggcg ctcgatgcgc gccgcgaagc gctcgcgcac gagctcgaga
     9661 atgaagcggt tgggtgcggt gagaacgagg gtgtcggatg catcgcctgc ctcgggagca
     9721 agcggccgga tccacgtatt gaactgctgc gcggggatct cctgctcgag acgacgcagg
     9781 caggcatccc agaggttttg catcttttcg ttggcctaaa gcgagcccga atcttacctt
     9841 cttggtgacg tgttatccac agatgaattg acaacgcgct tacgcgcacg ctctaatacc
     9901 gccctttttc ggatcggcca atggcgacga agcggaccta ccaaccctcg aagacgcggc
     9961 gcgcgcggcg ccatggtttt ctcgtgcgca gtcgcagcgc cggcgggcgc aaagtgctgc
    10021 gcagccgccg cgcacgcggc cggcgccgcc tcgggttgta gtcgcggcgg gcaagcgctt
    10081 cgggctggac gcgtcgcgcc gcctgaggcg aaaagacgag ttcgaaaatc tgctgcggca
    10141 cggttcacgg cgcagcttga acggctatac cttctatgtc gcgagacgcg agagcggcga
    10201 ggcgcggctc ggcttgctcg tgtcgcgcag acacgccgcg cgcgcggtcg tgcgcaacgg
    10261 tatcaaacgc tgcatccgcg aggcgtttcg cctaacgcag ggcgatctgg gagcgctcga
    10321 tgtcctggtg cgaccacctt atggcgctca gccctcggcg cagatggtcg cgcagttgcg
    10381 cgcgctcctt ccgcgcctga agccatgacg gtccgcgtcc ttcgcttcgc tttgaaaacc
    10441 tatcgatggt gcgtgcgccc actactggcg ccatgctgcc ggttctttcc aagctgctcc
    10501 gaattcgccg aagaggcgct cgtgcgccat ggattctggc gcggatgctg gctcaccgcg
    10561 cggcgcctct gtcgatgcgg tccgtggcat cccggcggac acgaccccgt tccctagacc
    10621 aaatggacac ccagcgactt atcctgctcg ttatcttcag cttctctttg ctcatgctct
    10681 gggaggcttg ggagaaggag aaacgaccca aaccggcgcc ttcgccggcc accgcgcagg
    10741 gcgtgccggt ccccgagaag ccggtagctg ctgctgcccc agcggcgaag ccctcggccg
    10801 ccatgccgga gggcgcaagt ccggcgaaag gcgagatcgt gcgcatcagc accgatctca
    10861 tcgcggtcga agtcgatacg ctcggcggca cactgaagcg agtcgaactc ttgcgccaca
    10921 aggattccaa ggatctttcc cggagcctgg tgctgctcgg gcccgagcac cactacgagg
    10981 cgcaaagcgg actggcgggc gagggcggac caaatcaccg cacgttttgg cgtgccgagc
    11041 cgggcgagcg tgcgctcgct gccgggcgcg atgaagtcac gctggttctc tccgcgacgg
    11101 ggaaggacgg cgcgcaggtg cgaaagacct acacgttcaa gcgtgacagc tacgtgatcg
    11161 acgtcgcttt ggaggtgcag aacgccggtt cggcgccgct cggcgcgtac gcgtacttcc
    11221 agctgacgca cgacggcaag cccgaaacga acaccaacac ggtcgccgaa accttcggcg
    11281 cgcagagctt catcggtttc gccgtctata ccgacgagcg caagttcgag aaggtgtatc
    11341 catcggatct cgacaaaggc cgctccgatc acgtcaaaca ggcgaccaac ggctggcttg
    11401 cgttcgtgca gcattacttc gtttccgcat ggctgccgcc gcagggcgtg ccgcgcgact
    11461 acgtaaccga gaagcgaccc gacggcatct acgctgcccg cgccatggtg ccgctcaatg
    11521 tcgcagccgg ttcgagcgca gcagtgaagg tgcccatcta cgtcgggccg caggagcagc
    11581 gccggctgca ggctgcggcg ccagggctcg atctcgtggt cgattacggc tggctggcta
    11641 tcattgcctg gccgctcttc tggcttctgg agaagtttca cgcgctttcg ggcaactggg
    11701 gcgtggccat catcctgctc actgtgctca tcaaaattgt cttcttcccg ctctcggcgg
    11761 cgagctacaa gtcaatggca aagatgaagc tgattacccc gcggctgacg aagctccgcg
    11821 agatgtacgc gcacgaccgg cagaagatga accaggccat gatggagctg tacaagaccg
    11881 agaagatcaa cccgctcggc ggctgcttcc cgatcctcgt ccaaattccg gtgttcatcg
    11941 ctctctactg ggtgctgctc gcggcgatcg agttgcgcca tgcacccttc atgctctgga
    12001 tcaaggacct ctccgccctc gacccgtact acgtgctgcc gatcctcatg acggccacca
    12061 tggtgttgca gacgcgcatg aacccggtgc caccggatcc ggtgcaggcg cgcgtgatgc
    12121 agatcatgcc gtttgtcttc agcatctttt tcttcttctt tcccgcgggc cttgtgctct
    12181 actggctcgt caacaacatt ctctccatcg cccagcaatg gcagatccag cgcatgttcg
    12241 accgcgacaa gcctgcccat gccaagcgat gagctgactc gcgacacgat cgctgcggtg
    12301 gcgacgccgg ccggccgcgg cggcatcggc gtgttgcgcg tgtcgggctc cctggcgctt
    12361 gcgatcgcaa agaaagtcgt gggacgcgca cccaagccgc gaacggcctt acacgcggta
    12421 ttccgcgacg ccggcggcga ggcgatcgac caaggcatcg cgctttactt tcccgcgccg
    12481 cattcttata caggcgaggc ggtgctcgag ctgcaaggac acggcggccc ggtcgtgatg
    12541 caggtactgc tcgctgcgtg tctcgacgcc ggcgcgcgcc tcgctgagcc gggcgagttc
    12601 acgcgccgcg cttttctcga aggtaagctc gacctggcgc aggccgaggc cgtggccgac
    12661 atcatcgacg ctgcgagccg cgaagcggcg cgctcggcgc tgcgctccct gagcggtgag
    12721 ttctcggcgg cgatcggcgc gctgcaaagc ggtctggtgg agctgcgcgc cttgaccgag
    12781 gcgatgctcg actttcccga ggaggacgtc gacaccttgg atcgcgagga tgccgtacgg
    12841 cgtctcgacg cgctgcgacg cggcttggat gggattcttg cccagagccg gcagggaagc
    12901 ctgcttcgtt cgggcattca tatcgtgatc gcaggccgac ccaacgttgg caaatccagc
    12961 ttgctcaacc gcctcgcggg cgaggagcgc gcgattgtcg cggcggttcc cggcaccacg
    13021 cgcgatgcgc tgcgcgagcc cattgagatc gatggcgttc cgctcgtagt cgtcgatacc
    13081 gcgggcctgc gcatttcgtc cgacgcagtc gagtgcatgg gcatcgagcg caccgagcgg
    13141 gagcttgcgc gggcagatgc ggcgattgtg gtcctcgatg cgacgggtgg agccgaggag
    13201 acgccgccga ttccgcccga cctcgcgcgc atcgaggtct acaacaaaat cgaccttgtg
    13261 cccggattcg ccgcgccgcg tggggcgatc gctctatcgg cgaagaccgg cgagggtatg
    13321 gatgcgttgc gcgccgccat ccttcgcgtc gcggggtggt cgtcgagcgg cgagccggtt
    13381 taccttgcgc gcgagcgcca cctgcgggcc ttggacgagg cacgaggcca tctcgagctc
    13441 gcggccaaag agcagcgctg ggagcttttc gcggaggaac tgcgcctcgc gcaccgcgcg
    13501 ctcggttcga tcacgggcca gttcgacgtc gagcacctgc ttggagaaat cttcgcgcgc
    13561 ttctgcatcg gtaagtaact gaaacataac actaatgtga cgcctaggct gaccggactg
    13621 gcgctttccg gtgaaccgcc gatgttagaa tcgctggcac acagtactct catgtcctcg
    13681 gcccagcccg tgctgggccg tttgcttcta gagagaccgc gcgatgaatg cacctagcac
    13741 cccattggac cagtcgcaag ccccatcctg ggtgcgacat cgacggttgc gtcaatgggt
    13801 tgccgaggtc gcacgcatgg ccaagccgga gaccatcgtg tggtgcgatg gctcgcaggc
    13861 agaatacgac cggctttgcg gtgagctcgt cgccgccgga actttcatcc gtctgaacga
    13921 ccggctgcgg ccgaactctt atctcgcgcg ttcgcaccca agcgacgttg cgcgcatgga
    13981 ggaccgcacc ttcatctgca gccagaagag agaagacgcc ggtccgacca acaattggat
    14041 cgatccccgc gaaatgcggg ccacgctgac ccgcctattc gatggctgca tgcgtggacg
    14101 caccatgtac gtcatgccgt tttccatggg gcccatcggc tcgcccatcg cgcaggtcgg
    14161 cgtcgagatc tccgattcgg cttacgtggt cgctaacatg cgcatcatga cgcgcatggg
    14221 acgcgcggtc gccgaatggc tcggcgagga ccggcctttc gttccttgcg tacattcggt
    14281 tggtgcgcca ctcgcgcctg ggcgcaagga tgttgcgtgg ccgtgcagtg agaaggagaa
    14341 gtggatcgtt catttcccgg agacgcggga gatctggtct ttcggctcgg gctacggcgg
    14401 caacgctctt ctcggcaaga aatgcttggc gcttcgcatc gcctcggtca tggcgcgcga
    14461 cgaaggctgg ctcgccgagc acatgctgat cctcggcgtg caggcgccgt ccggcgagaa
    14521 gacttatatg gcggcggctt tcccttccgc ctgtggcaag accaacctcg cgatgctgat
    14581 ccccccggca ggcttcagtg gctggaaggt cacgaccatt ggggaagaca ttgcgtggat
    14641 caagccgggc ccggacggcc ggttccgggc aatcaacccc gagtacggcc tgttcggcgt
    14701 tgccccgggc acttcgcagc acaccaatcc caacgcgatg cggatgctgc aggcgaacgt
    14761 gattttcact aacgtggcca tgactccgga gggcgatgtc tggtgggaag gaatgaccga
    14821 cgagccgccg gcgcggctga tcgattggca gggaaaggac tggaccccgg atagcggtcg
    14881 cttggccgcg catgccaatg cgcgttttac cgtgcccgcg gcccaatgcc cgtcgatgga
    14941 ccccgcttgg gaagatccgg ccggcgtccc gatctctgcg ttcatcttcg gtggccggcg
    15001 ctcgaccacc gtacccctcg tagtcgaagc gccgagctgg gaagaaggca cctatatggc
    15061 agccaccctc ggctccgaaa ccaccgcagc gattaccggg aaagtgggcg tagtgcgccg
    15121 tgacccgatg gcgatgctcg cgttttgcgg ctacaacatg ggcgactact tcgcgcactg
    15181 gctgaaaatg ggtcgcgcgg taaagcagcc gccgaaaatc ttccgcgtga actggtttcg
    15241 gcgcgacgcc ggcggcaagt tcctgtggcc gggcttcagc gaaaacatgc gcgtgctggc
    15301 atggatcgtc gagcgctgtc agggaagcgc acgcggccag gaaacgccgc tcggcttgat
    15361 gcctcgctac gaggacctga actggagtgg gctggaaaag gtgagcgcca cgcgcttctc
    15421 cgagctcaca caggtggatg cggccgcatg gaaagaggaa ttgcgctcgc acgacgagct
    15481 cttcgccaag ctcggtgccc ggcttccggc tgcgctcgaa gcgcgccgcg gccgcatgca
    15541 cgaaaagcta gcggcgtaaa aagaccctcg caccggcggc cgagagcagc gccgccgccg
    15601 cgcccgcaac gaacgccgcc gaccaaggta ggcgctcgag cagcatcccg ccaaaggcaa
    15661 gtccgatccc gacaccagcg agcagggcgc tcgtcgtcca tgtgaaagcc tcggtggagt
    15721 gctcggtcgc cgcactcttc gtgacgagca tggattgaat aatcaaggcg ggcgcaatcg
    15781 cgacccctgc taatagcgac cagaccgcaa acacccaagg ttgccaaggc gccgcgagca
    15841 cggcgagccc gccggccgtc agggcgagtg ccgctgcgaa ctggcgcgca agcggcgcgt
    15901 gccacgcgcg gcttccataa gcaaggcccc caagggcgct gcccgcgctc atcaggccga
    15961 gcagcacgcc agcaagggcg gcgtcgcttg tctcgacggc gtacgccgta atgccgattt
    16021 cgaggaagcc gaaggccgtg gcgaagcaga gcacgattgc aaccagatgc gcaaagtcgc
    16081 gccgcgcgag cggaccgagc aggcccgtgg ctgagcgacg ctcgatgcgc caaccctgta
    16141 gcgccggcga gcgcaggaac acgagcgtgc cgacgagcgc acaaccggcg gagaaccaga
    16201 cggccaatgc gggggcggcc gtcgcggcaa agaccgcgac cacaaggggg ccgacgatga
    16261 aaatcacctc gatcagcacc gattctgcag agaacgccgc cgataaaagc gcttcgtcgg
    16321 tgagcgcacg ccgcatgtaa gtccgcatgc agaccgtaat tggtggaaag ctggcccctg
    16381 ccgccgccgc cgccgcgatc tcaagccaag gcacggcagc ctctgagccg tagatgagga
    16441 acgctagcgc tgatggaaat gcgagagcgc aaaagcgcag caccgcgcgc gggccgtgcc
    16501 ggtcgatcat gcgtccaagg cccggggcgg cgagcgcgag gccgctgaca tagcacccgg
    16561 ccgcgaagcc gccccgggcg aacgaacccg tggcggactg aacgagaaca agaatagcga
    16621 gccccgttac gccaatcggc aggcggccga tgatggagat gagaaacgtg cggcgtaagg
    16681 gagcgctgga aaaagcggtg cgatagcggg cgagtgagag catgatgcgg cgcagcaaag
    16741 atcataggtt ccacgtgaaa cgcgcacccg gtagtttcac gtgaaacgat cacgtaaaat
    16801 tgcgcacccc atggtttttc cgcgcgagtt cgacgtcatc gtggtcggtg gcggtcacgc
    16861 cggtaccgag gcagcattgg ctgcggcccg cctcggctgc gcgacgctgc ttcttacgca
    16921 cagcatagag accttgggac agatgtcgtg caacccgtcc atcggaggca tcggcaaagg
    16981 tcatctggtg agggagctcg atgcgctgga cggggtcatg gcgctcgcta ctgatgaggc
    17041 cgggatccag tttcgcacgc tgaacgcttc caagggaccg gctgtccgag cgacccgggc
    17101 gcaggctgac cgggtgctgt accggaacgc gatccggcgt cgacttgaga accagcccaa
    17161 tctgtggctg ttccaacagc aagtcgacga catcctgctc gaaggcgccc gcgcggtcgg
    17221 cgtcgcaacg caggtcggcg tagcgttccg tagccgcacg gttgtcttga cgaccggtac
    17281 cttcctttcc ggactcatcc acgtcgggct gcaacactat caggccggac gcgccggtga
    17341 tccgcccgcg ctgagactgg ccgcgcgact gcgcgagctt cggctgccgg ttggacgact
    17401 gaagaccggc acgccgcccc ggctcgacgc ccgctcgatc gatttcagcc tcatggagga
    17461 gcaacgcagc gattcaccgg agccggtgtt ctcgttcctc ggccggcggg agatacatcc
    17521 ggcgcagcga ccgtgttgga tcgcgcatac caacgagcgc acgcacgaga tcatccgcgc
    17581 ggccctcgac cgctcgccga tgttcacggg cgccatccag ggggtcggtc cgcgctactg
    17641 cccctcgatc gaggacaaga tccatcgctt cgtcggcaag gcgagccacc agatcttcct
    17701 cgagccggaa ggcctgacta cgcacgagat ctacccaaac ggaatctcca cgagcctgcc
    17761 gtttgacgtg cagctggagc tcgtgcgctc gatccgcggc ctggagaatg cgcacgtcgt
    17821 gcgccccggc tacgcgatcg aatacgacta ctacgacccg cgccatctgc gcacgtccct
    17881 ggagaccaag agcatcagcg ggctgttttt cgccggtcag atcaacggca ccaccggata
    17941 cgaggaggcg gcggcgcagg ggttactcgc cggcttgaac gccgggctgc agacgctggg
    18001 gcgcgatgcc tggtgtccga agcgcgacga ggcctacctc ggcgtgctcg tcgatgatct
    18061 cgtgacccgc ggtgtatccg agccctaccg gatgtttacg agccgggccg aatatcgcct
    18121 tatgctgcgc gaggacaacg ccgatttgcg actcaccgag acgggccgca ggctgggatt
    18181 ggtggcggac gagcgctgga tggcgttcgc gcggaagcgc gatgcgatcg cacaggaaac
    18241 cgcgcgcctc aaaggcgtgt ttgtaagcgc tggccctcag gaaaagcggc cagcctacga
    18301 gctacttcgt cggcctgagg tgcgccacgc cgatttgccg caggacggcg tggtcgaccc
    18361 gatggtcgcc gagcagatcg agatccaagc gaagtacgag ggttatatcg agcgtcagcg
    18421 tgatgaggtc gcgcggcgcg gggagtttga ggatcgcgcc ttaccctccg agttggatta
    18481 ccgcgacgtg cggggccttt ctgcggaagt ccagcagaag ctcaattggc accgccccga
    18541 aacgatcggc caggcggcgc gcattgccgg gatcacgccg gcggcgatct cgcttctgct
    18601 cgtccacctg aagaggggat tcggcaagcc cgaaaagaag cgcgcatgac gcccggcgca
    18661 gcgctcgatc aggggttagc gcagctcggg ctttccctgc caacgagcgc acccgaagta
    18721 ctccttaagt atcttgcttt gctttcgaag tggaaccgga cctacaacct gaccgcgatt
    18781 cgagacccac tcgcgatggt ggctcaccat ctgctcgatt ccctggcggt ccttccgtat
    18841 ctgcccctgg gtgatcacgc gcgtgtcgcg gacgccggtt ctggcgccgg gctaccgggg
    18901 attccgatcg ccattgcccg gcccagctgg cacgtcgcac tggcggaatc gagccagaag
    18961 aaagccgcgt ttcttcgtca agccacgatc gaactcgcgc tcgcgaacgt ggaagttcac
    19021 gaaggccgcg tcgagcgctg gcagccacgg ccgttgttcg atctggtcat ttcgcgcgcc
    19081 tttgccgccc tgggcgattt catcgcggca tgccgccatc tcgtgcaacc cgctggagtg
    19141 ctcgcagcca tgaaaggcgc ggcgcctgaa gatgcgccgc gcgagtgtca gctcgtgcgg
    19201 ctcgacgttc cgctgctcgg cgcgcaacgc caccttgtgc tatgccccgc gtcctcgcag
    19261 tcgtgaatca gaagggtggc gtcggcaaga cgacgaccac ggtgaatctg gccgcggcgc
    19321 tcgcccaggc aggtcgcagg gtgctgctcg tcgatctcga cccgcagggc aacgcaacca
    19381 tgggaagcgg cgtcgataag cgggcggcgg tgcgcaccgt gtaccacgcg ctgctcggct
    19441 tgggcgaact cgccggcatc cgcacccgcg ccgagcgcgg cggctacgat gtcgtgccag
    19501 cgaaccgcga cctggccggc gccgaggtcg agctcgtgga actgcccgcg cgcgaaacgc
    19561 gcctgaaggc ggcgctcgag cgcgtggcgg acgattacga ctacatcctg atcgattgtc
    19621 ccccgtccct gagcctgctc accgtgaacg cgctcgccgc ggcgcagcgc gttcttatac
    19681 ctatgcaatg cgagtactac gcgctcgaag gactgtccga cctcgtcggc accattaagc
    19741 gcgtgcgcgc caatctcaat cccaccctgg agatcgcggg gttgctgcgc acgatgtacg
    19801 accctcgcaa caccctgtcg cagcaggtgt cgcgcgagct cgaatcgcat ttcggcgaca
    19861 aggtgtttcg tacgctcgtg ccgcgcaacg tgcgcctcgc agaagcgccg agctatggcg
    19921 tgcctgccgt agtctgggat gcgagttcca aaggagcgca ggcgtatgtc gcgctcgcga
    19981 gcgaagttct ggagagccca tgaaaccaag aggacttggc cgcggcctgg acgcgctgct
    20041 cggcggcgat gaacagccgc gcgatgcgct gctcacgctg cccgtcggac gcattcggcc
    20101 cgggcgttac cagccgcgca cgaagatgga ccagcaggcg ctcgccgagc tcgccgcgtc
    20161 catccgctcg caggggctga tgcagccgct tctcgtgcgt ccggtggatc gcgatcgcta
    20221 cgagctcgtc gcgggcgaac ggcgctggcg cgcggcgcag atggccgggc tggaagaagt
    20281 gccggcgctc gtgcgcgaga tccccgacga ggcggcgctc gccatgtcgc tcatcgagaa
    20341 catccagcgc gagaatttga acccgatgga ggaagccgcc ggcatccagc gtctcgtcga
    20401 cgagttcagg atgacgcacg agcaggccgc cgacgcggtg ggccgctcgc gcagcgccac
    20461 gacgaacctg ctgcgcttac ttaaactcgc gcgcccggta caggagatgc tgatggaggg
    20521 ggcgctcgag atgggacacg cgcgcgcgct tctcgcgctc gatggtgcgc gtcagatcga
    20581 aagcgcgaag cgcgtcgccg cgaagggcct ctccgtccgc gccaccgagg cgctcgtgca
    20641 taccatgctg cgcggcccgc aggcgcgcac gaagcggcgc gaccgggatc tcgcgcgcct
    20701 ggaagaggaa acttccgagc gcctcggcac cacggtggag atccgcgccg gcaagaaggg
    20761 cagcggcaga atcgttttgc attactcgag cctcgagcat ctcgaagagc tccttgccaa
    20821 gctgaagtga ccggcgcttg cacgcatgga atcccgacgc aataccgact gtttcgtgac
    20881 gactccatgg tcgtttgagc tatacttcgc cccgctttgc gcctgcctct gagcaagccg
    20941 attcgcatcg tgctcaagtg gcagctggtc gcgaccgcgg tcgcgacctt ggcggcggca
    21001 ctggtcgcgg gacaacacgc agcagcttct gccgcggccg gcggcgcggt gagcatcgtg
    21061 gccgggatgg cggctgcgac aattgccgcg ctcgggagag cgaagtcggc aggcagtgtt
    21121 cttgcgagcg cgctgcgcgc cgaggccgtg aagctgggcc tggcgctggt tctgctttgg
    21181 ctcgtgctgg tgaactaccc gcaggcggcc gtcggcgtgc tactggcgac gttcgtcgtg
    21241 acgatgctca ttttttcgat ggcttttttt gttcgcgagt actgacgcaa ccgatgatgg
    21301 ccgccggcac cgagctcacc ccttcgcagt acatacagca ccacctcacc ttctttacca
    21361 agc
//
LOCUS       DASTPD010000022         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_825265, whole genome shotgun sequence.
ACCESSION   DASTPD010000022 DASTPD010000000
VERSION     DASTPD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825265"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFR57_01025"
     CDS             <1..501
                     /locus_tag="VFR57_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0257990.1"
                     /translation="PAFLADLNSLYFFCFAIFIVVILFLWRLQSSPFGRVLAAIRQNE
                     TRARFVGYHVWAYKWLAFTLSALVAGLAGGLFALAQQSAYPNVMSLHQSGFVVMMALI
                     GGGLVSFWGPVLGVVLFFVARDLLGAYTETWLLWYGLLFMLVVLFKPEGLAGMLRKDV
                     RSHASA"
     gene            476..1195
                     /locus_tag="VFR57_01030"
     CDS             476..1195
                     /locus_tag="VFR57_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257991.1"
                     /translation="MFEATHLHKRFGDHVVLEDVSMSFAEGRLTGIMGPNGAGKTTCF
                     NLLTGRYAPDRGRVRFAGRDITGLPPRSIARLGISRSFQVMNLFDEYSALDNVLVATP
                     EVRARGYSMLGNFSGKTDVLERVGLADKAHVRAKDLSYGERRALEIGVALAARPQLLF
                     LDEPTAGLGTEATARIAALIAALKRSLTIVLIEHDMRFLFGLADRIYVMHWGQVIAEG
                     TPAQLRANPWVQRSNLGNVGG"
     gene            1182..1877
                     /locus_tag="VFR57_01035"
     CDS             1182..1877
                     /locus_tag="VFR57_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257992.1"
                     /translation="MLAVREIHAYYGETQALFGVSLEVAAGEVVALLGPNGAGKTTTL
                     RSILNLSKVRKGDIRFDGADITGMATHEIARRGIGWVPDDRRIFPTLTVERNLSIAKK
                     RTRWRAWSEKECFEIFTALEHLRHRECENLSGGEMQMVSLSRALLGSPGLVLLDEPSQ
                     GLAPRMVQQVVAVIKTLQSAGIAVLLVEQNALSALAVAERVYVMDKGRVVHQGPAPGL
                     LKDTSLRTRLLGV"
     gene            1874..2470
                     /locus_tag="VFR57_01040"
     CDS             1874..2470
                     /locus_tag="VFR57_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257993.1"
                     /translation="MTLLSVEGLAKEYRRRWKTTFRLEASFTVEKPEIVGVMGPNGSG
                     KTTLFELITGSNTPSAGRVLVAGQDIHRVKARSRDRLAIHYHQSYQVRRFRKLKPDFL
                     LDTANSAYPMVHLFDEPQFNTQDGYIGFMLDFFRRLRSEDRVVFLCLHPTERYHLEIL
                     REVCERFVFVEKGRISLHPNFAALEADARVSAYLGQSA"
BASE COUNT          403 a          823 c          817 g          459 t
ORIGIN      
        1 ccggcgttcc tcgccgatct caattcgctc tacttcttct gcttcgcgat cttcatcgtc
       61 gtgatcctct tcctctggcg gctgcagagc tcgcccttcg gccgggtgct cgccgcgatc
      121 cggcagaacg agacccgcgc gcgtttcgtc ggctaccacg tctgggcgta caagtggctc
      181 gcgttcacgc tttcggcgct cgtcgccggc ctcgccggcg gcctcttcgc gctcgcgcag
      241 cagtcggcgt atccgaacgt gatgagcctg caccagtcgg gcttcgtggt gatgatggcg
      301 ctgatcggcg gggggctggt gtcgttctgg gggccggtgc tcggcgtggt gctcttcttc
      361 gtcgcgcgcg atctcctcgg cgcctacacc gagacctggc tcctgtggta cggcttgctc
      421 ttcatgctgg tcgttctttt caaaccggaa ggcctggcag gaatgctcag aaaagatgtt
      481 cgaagccacg catctgcata agaggttcgg ggatcacgtc gtgctcgagg atgtgagcat
      541 gagcttcgcc gaaggccgcc tcaccggcat catgggcccg aacggcgcag gcaagacgac
      601 ctgcttcaac ctcctcaccg gccgctacgc gcccgaccgc gggcgtgtgc gcttcgcagg
      661 ccgcgacatt accggccttc cgccgcgctc gatcgcgcgg ctcggcatct cgcgctcgtt
      721 ccaggtgatg aatctgttcg acgagtacag cgcgctcgac aatgtgctgg tcgccacgcc
      781 cgaggtgcgc gctcgcggct attcgatgct gggaaatttc tccggcaaga ccgatgttct
      841 cgagcgcgtc ggcctggcgg acaaggccca tgtgcgggcg aaggatctct cctacggcga
      901 gcgccgcgcg ctcgagatcg gggtggcgct cgcggcgcgg ccgcagcttc tgttcctcga
      961 cgagccgacc gcgggtctcg gcaccgaggc cacggcgcgc atcgccgcgc tcatcgccgc
     1021 gctcaagcgc tcgctcacca tcgtgctgat cgagcacgat atgcgcttcc tcttcgggct
     1081 cgccgaccgg atctacgtca tgcactgggg gcaggtgatc gctgaaggca cgccggcgca
     1141 gctgcgcgcc aatccctggg tccagcgctc gaacctcggc aatgttggcg gttagggaaa
     1201 tccacgccta ttacggcgag acgcaggcgc tgttcggcgt ctcgctcgag gtcgcggcgg
     1261 gcgaggtggt cgcgctcctc ggcccgaacg gcgccggcaa gaccacgacg ctgagatcga
     1321 tcctgaactt gtccaaggtg cgcaaaggag acatccggtt cgacggcgca gacattaccg
     1381 gaatggccac gcacgagatc gcgcgccgcg gcatcggctg ggtgcccgac gaccgcagga
     1441 tctttcccac gctgacagtg gagcgaaatc tttccatcgc gaagaagcgc acgcgctggc
     1501 gcgcgtggag cgagaaggaa tgcttcgaga tctttaccgc gctcgagcat ctccggcacc
     1561 gcgagtgcga aaacctctcg ggcggcgaga tgcagatggt gtcgctgtcg cgcgcgctcc
     1621 tcggctcgcc gggcctggtg ctgctcgacg agccgagcca gggcctcgcg ccgcgcatgg
     1681 tccagcaagt cgttgctgta ataaagacac tccagagcgc ggggatcgcg gtgctgctcg
     1741 tcgagcagaa cgcgctcagc gcgctcgcgg tggccgagcg cgtctacgtg atggacaagg
     1801 gccgggtggt gcaccaggga ccggcgcccg ggcttctcaa ggacacgtcg ctgcggacga
     1861 ggcttctcgg cgtatgaccc tcctcagcgt cgaagggctc gccaaggaat accgccgccg
     1921 ctggaagacc acgttccgac tcgaggcgag cttcacggtg gagaagcccg agatcgtcgg
     1981 cgtaatgggg ccgaacggct cgggcaagac cacgctcttc gagctcatca cgggctcgaa
     2041 caccccgagc gccggccgcg tgctggtggc ggggcaggac atccaccggg tgaaagcgcg
     2101 cagccgggac cgcctcgcca tccactacca tcagtcatat caggtacgtc gtttccgcaa
     2161 gctgaagccg gatttcctgc tggacaccgc gaacagcgcc tatccgatgg tccacctgtt
     2221 cgacgagccg cagttcaaca cccaggacgg ctacatcggc ttcatgctcg acttcttccg
     2281 gcgcctgcgg agcgaggata gggtggtgtt cctgtgtctg catcccaccg agcgctacca
     2341 cctcgagatc ctgcgggagg tatgcgagcg cttcgtgttc gtcgagaagg gtagaatttc
     2401 cctccatccg aacttcgctg cgctcgaggc cgacgcgagg gtctccgcct acctgggaca
     2461 gtctgcatga acaagcctgc tgaattgcga ttggaaacgg tt
//
LOCUS       DASTPD010000023         6698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_825403, whole genome shotgun sequence.
ACCESSION   DASTPD010000023 DASTPD010000000
VERSION     DASTPD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825403"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFR57_01045"
     CDS             <1..618
                     /locus_tag="VFR57_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257994.1"
                     /translation="RPANNVLLTGARGTGKSSIVKGLLNKYARQGLRLIEVEKNDLVD
                     LATIVDHVAARPERFILFCDDLSFHGAEEGYIALKVALDGSISTTSENLLIYATSNRR
                     HLMPEYMAENLETKYLGDEIHPGETVEEKISLSERFGLWVTFYPFDQDEYLEIVGQWL
                     KWFDSSLNEKAKEEALQWALQRGSRSGRVAYQFARDWAGRHSKKR"
     gene            615..1511
                     /locus_tag="VFR57_01050"
     CDS             615..1511
                     /locus_tag="VFR57_01050"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEU0257995.1"
                     /translation="MIEVAAAVVQRPDGAFLLAQRPPGKVYAGYWEFPGGKVEHGEPP
                     HRALARELHEELGIDIGPAYPWITRVYTYPHGTVRLNFFRVFDWRNDPHPREDQALAW
                     QASDAPMAAPMLPANAPVLASLALPVEYAVTDAAHYGIAAMLSRLGQRLEQGLKLVQV
                     REPGLGAEQRDLFTSQVIGLAHRHGCKVMVKSAFPGADGIHFKASELASLKERPRGML
                     AAASCHSRAELEQAMELELDFAVLGPVKDKPTALGWARFADLARGASIPVYAIGGLTC
                     SEMQDAWSAGAHGIAMIRGSWN"
     gene            complement(1492..1671)
                     /locus_tag="VFR57_01055"
     CDS             complement(1492..1671)
                     /locus_tag="VFR57_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEU0257996.1"
                     /translation="MRCPRCGTRTEYSRENKWRPFCRERCRTIDLGRWAAEDYRVPEE
                     KSRSDPDSSPNSNSP"
     gene            complement(1677..2447)
                     /gene="zapD"
                     /locus_tag="VFR57_01060"
     CDS             complement(1677..2447)
                     /gene="zapD"
                     /locus_tag="VFR57_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEU0257997.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARPEPHDHHVALLTL
                     FEIMEVASRADLKSDLLQELERQKQVLLSFRNNPDIAEQALASVLRDIEQASQALFSM
                     TGKIGQYLRENDWLMSIKQRTGIPGGACEFDLPSYHYWLHRPSEERTGQLAAWTGPLY
                     PLRDGSAIVLRILRESGKPQAMSAPQGMFQQMLGGKTAQMLRLRLDPKMPCVPEISAN
                     KYVLNIRFVIQDGANQSPESRVRTADWDVGFELTFCNL"
     gene            complement(2444..3037)
                     /gene="coaE"
                     /locus_tag="VFR57_01065"
     CDS             complement(2444..3037)
                     /gene="coaE"
                     /locus_tag="VFR57_01065"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059619.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0257998.1"
                     /translation="MVGLTGGIGSGKSAAAADFAALGATVVDTDVIAHELTAKGGAAM
                     AGIEKLFGPEAVSGEGSMNRKKVRERVFADPAAKRKLEALLHPMIREESARRIAAATG
                     PYVVHVVPLLIESPDYRKRVDRVLVVDCSEETQVARVRARPGLSENEVRAIMRTQATR
                     AERVAAADDVIDNGGSREALHEQVAALHQKYLQFAGR"
     gene            complement(3043..3906)
                     /locus_tag="VFR57_01070"
     CDS             complement(3043..3906)
                     /locus_tag="VFR57_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0257999.1"
                     /translation="MTAAGWVLQPAFLPWAALVLGLCVGSFLNVVIHRLPKMLERGWR
                     EECAELAGQPLPQEPPYSLMAPRSACPSCGHRVRAWENVPIASWLVLRGRCSACRGRI
                     SAKYPLVEALAGIGAAYAAWRFGATATALGAAIFIWFTIALAFIDQETGLLPDTLTLP
                     LLWLGLLLNLTQAFTSIQEAVIGAAAGYLSLWLVFHAYRLATGKEGMGYGDFKMNAAV
                     GAFLGWKLLPLVILLSSLVGLAFGALQMFAARGRWDASFRFHFGPYLAIAGIVAMFWG
                     EPILRWYLQRL"
     gene            complement(3911..5131)
                     /locus_tag="VFR57_01075"
     CDS             complement(3911..5131)
                     /locus_tag="VFR57_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0258000.1"
                     /translation="MATAAAARGPKQFTFSWEGRDKAGKTIRGELRAVSEAAVNATLR
                     RQGIVVQKVKPVKTRGGGKVTEKDIALFTRQLATMMKAGVPLLQSFDIVSKGASNAAV
                     AKLLTDIKTDVETGSALASAFRKFPLHFDALFCNLVQAGEQAGILETLLDRLATYKEK
                     ILAIKSKIKSAMFYPIAIIAVAFIITAVIMIFVIPAFKEVFTNFGADLPAPTLVVIGI
                     SDWFVANWYIVFPVLGGAIYGFLEAWKRSVAVQIFMDRFTLRLPLFGDLVRKSAIARW
                     TRTLSTMFAAGVPLVEALDSVGGAAGNYIYAQATRQIKQEVSAGTSLTMAMQNSNVFP
                     NMVVQMVSIGEETGSLDAMLGKVADFYEQEVDDAVEALSSLMEPAIMVVLGVLIGGMV
                     IAMYLPIFKLGQAL"
     gene            complement(5217..6539)
                     /locus_tag="VFR57_01080"
     CDS             complement(5217..6539)
                     /locus_tag="VFR57_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEU0258001.1"
                     /translation="MELLLLASLILLNGALAMSEVALLTARKPRLETRAKQGDKLAAS
                     ALALARDPTRFLSTIQIGITSIGILNGIVGEAFLSAPVSAWLQSTLPITAKTANVVST
                     VGIVVTITYVSIVVGELVPKRLGQHNAEGIARLVAWPMRLLAKMSAPFVALLSTSTNA
                     ILNPLLALARMRGGEDEGEMSPEEIRTILLEYSNVLPKKHVSILLNLFDVGAVTVQDI
                     MVPRAKIESLSLDDDMETIVRQIVTSHHGRLPVFRSALGDVVGVLHLKKVLPALPTGT
                     LDKPTLESVLSEPYFVPATTPVLAQMQYFQENRERIALVVDEYGELMGLLTLEDIIEE
                     IIGKFTTSLPTAGQALAWDRSGAATAEGATPVREVNRALALSLPTDGPKTLNGLILEY
                     LQDIPEADVSIKIGTVPMEIVHAQGRTIKTVRLFRPVKNVENTEPSEA"
     gene            complement(6552..>6698)
                     /gene="ccsA"
                     /locus_tag="VFR57_01085"
     CDS             complement(6552..>6698)
                     /gene="ccsA"
                     /locus_tag="VFR57_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0258002.1"
                     /translation="TFGLLLAGRWRYGWRGRTALRWTLTGFVMLLLAYVGSRFVLEVL
                     LGRS"
BASE COUNT         1190 a         2139 c         2209 g         1160 t
ORIGIN      
        1 agaccggcga acaacgtgct gctcaccggc gcgcgcggca cgggcaagtc gtcgatcgtc
       61 aaggggctgc tgaacaagta cgccaggcag gggttgaggt tgatcgaagt ggagaagaac
      121 gacctcgtcg atctggcgac catcgtcgac cacgtcgctg cgcgccccga gcggttcatc
      181 ctgttctgcg acgatctctc tttccacggc gccgaggaag ggtacatcgc gctcaaggtc
      241 gcgctcgacg gctcgatatc gaccacctcc gagaacctcc tcatctacgc gacttccaac
      301 cgccgccacc tgatgcccga gtacatggcc gagaacctcg agaccaaata cctcggcgac
      361 gagatccatc ccggcgagac cgtggaggag aagatctcgc tttccgagcg cttcggcctg
      421 tgggtgacct tctatccctt cgaccaggac gagtacctgg aaatcgtggg ccagtggctg
      481 aaatggtttg actcctctct taatgaaaag gcaaaagagg aggcgctgca atgggcgctg
      541 cagaggggct cgcgcagcgg ccgggtcgcc taccagttcg ctcgcgactg ggccggcagg
      601 cactccaaga aaaggtgatc gaggttgccg cggcggtggt tcagcgcccc gacggcgcgt
      661 ttctgcttgc gcagcgcccg ccgggcaagg tctacgcggg atactgggag ttccccggcg
      721 gcaaggtgga gcacggcgag ccgccacacc gggcgctcgc ccgcgagctg cacgaagagc
      781 tcggcatcga catcgggccg gcgtacccgt ggatcacacg cgtatatacc tatcctcacg
      841 gtacggtgcg cctgaacttc ttccgagttt ttgattggcg caacgacccg catccgcgcg
      901 aagaccaggc gctcgcgtgg caggcttccg acgcccccat ggctgctccg atgctgcccg
      961 ccaacgcgcc ggttctagcc tcgctcgcgc tgccggttga atacgccgta acggacgcgg
     1021 cgcactacgg catcgcggcg atgctttctc ggctgggaca gcggctggag caggggctca
     1081 agctggtgca agtgcgcgag ccgggcctgg gcgccgaaca acgggatctg ttcacctctc
     1141 aggtgatcgg gctcgcgcac cgtcacggct gcaaggtgat ggtgaaatcg gccttcccgg
     1201 gcgcggatgg cattcatttc aaggcttctg agctggcttc actgaaggaa agacctcgtg
     1261 ggatgcttgc ggcggcctcg tgccacagcc gagcggagct agagcaggcc atggaactcg
     1321 agctggactt cgccgtcctc gggccggtga aggacaagcc gaccgcgctg ggatgggcgc
     1381 gcttcgccga ccttgctcgc ggcgcgagta ttccggtcta cgcgatcggt ggattgacgt
     1441 gctccgagat gcaggacgcc tggagcgcgg gggcgcacgg gatcgcaatg atcaggggga
     1501 gttggaatta ggagaagaat cggggtctga ccgcgatttt tcttccggca cgcggtagtc
     1561 ctcggcggcc cacctgccca gatcgatagt tctgcagcgc tcgcggcaga acggacgcca
     1621 cttgttctcg cgcgaatact cggtgcgcgt gccgcagcgt gggcagcgca cgattcctac
     1681 agattgcaaa acgtgagttc gaagccgacg tcccagtccg cggtgcgcac gcgggattcg
     1741 gggctctgat tcgcgccgtc ctggatgacg aagcggatgt tgaggacgta cttgttggcg
     1801 ctgatctccg ggacgcaggg catcttggga tcgaggcgca gccgcagcat ctgggccgtc
     1861 ttgccgccga gcatttgctg gaacatgccc tgcggcgccg acatggcctg cggcttgccc
     1921 gactcgcgca gaatccgcag gacaatggcc gagccgtcgc gcagtggata gagcggcccg
     1981 gtccaggccg ccagctgccc ggtgcgctcc tcggatggcc ggtgcagcca atagtggtac
     2041 gacggcaggt cgaactcaca cgcgccgccg gggatgccgg tgcgctgctt gatcgacatc
     2101 agccaatcgt tctcgcgcaa atactggccg atcttgccgg tcatggagaa cagcgcctgc
     2161 gacgcctgct cgatgtcgcg cagaacgctg gcgagcgcct gctcggcgat gtcgggattg
     2221 ttgcggaagg agagcagcac ctgcttctgg cgctcgagct cctgcagaag atcagacttt
     2281 aggtcggcgc ggcttgcgac ctccatgatc tcgaacaggg tcagcagcgc gacgtggtgg
     2341 tcgtgcggct ctgggcgcgc gatgaagtga cgcgagcgct cgaacaggtc ctccagccgc
     2401 agcagcgtgc ggatgcgctc gttgagcggg tattcgtacg tgatcaccgg cctgcgaatt
     2461 gtaggtattt ttgatgcagc gcggcgactt gttcgtgcaa cgcctcacgc gagccaccgt
     2521 tgtcgatcac gtcgtcggcc gccgctacac gctcagcgcg cgtcgcctgg gtgcgcatga
     2581 tggcgcgcac ttcgttttcg gaaagcccgg ggcgcgcgcg tacgcgtgcg acctgcgttt
     2641 cctcggagca gtcgaccacc aacacacggt cgacgcgctt acggtagtcg ggcgactcga
     2701 tgagcagcgg cacgacatgc acgacgtaag gcccggtggc ggccgcgatg cgccgggcgg
     2761 attcctcgcg gatcatcggg tgcagcagcg cctcgagctt gcgcttcgcg gccgggtccg
     2821 cgaacacgcg ctcgcgcacc ttcttgcggt tcatcgagcc ctcgccgctc accgcctccg
     2881 ggccgaagag cttctctatg ccagccatgg cggcgccgcc tttcgccgtg agctcatgtg
     2941 caatgacgtc cgtatcgaca accgtggcgc cgagcgcggc aaagtcggcc gcggcggcgg
     3001 attttccgct gccgatgccg cccgtaagtc ccaccacgaa ggtcacagcc gctgcaggta
     3061 ccagcgaagg atgggctcgc cccagaacat ggccacgatg ccggcgatgg cgaggtacgg
     3121 gccgaaatgg aagcggaagc tcgcgtccca gcgaccccgg gcggcgaaca tctgcagcgc
     3181 accgaaagcg agaccgacga gcgaggaaag caggatcacg agcggcaaca gcttccatcc
     3241 gaggaatgcg cccaccgccg cgttcatctt gaaatcgccg taacccatgc cttccttgcc
     3301 agtggcgagc ctgtaagcat ggaatacgag ccatagcgat aggtagccgg cggcggcacc
     3361 gatgaccgcc tcctgtatgg acgtaaaggc ctgggtgagg ttcagcagca agcccagcca
     3421 caacaggggc agggtcaggg tgtcgggcag aaggcccgtt tcctggtcga tgaacgcgag
     3481 cgcgatagtg aaccagatga agattgcggc tccgagcgcg gtggcggtcg cgccgaagcg
     3541 ccacgcggca tatgcggcac cgatcccggc gagagcttcg accagcgggt acttcgcact
     3601 gatgcgcccg cggcacgcag agcacctgcc gcgcagcacg agccagcttg cgatcggcac
     3661 gttttcccag gcgcggacgc ggtgcccgca cgaggggcac gccgagcgcg gcgccatcaa
     3721 gctatacggc ggctcctgcg gcagcggctg cccggcgagc tcggcgcact cctcgcgcca
     3781 gccgcgctcg agcattttcg gcagccggtg gatcacgacg ttgagaaacg agccgacgca
     3841 cagcccgagc accagcgccg cccacggcag gaaggccggc tgtagcaccc agccggccgc
     3901 cgtcatgatt tcaaagagct tgccccagct tgaagatcgg caggtacatc gcgatcacca
     3961 tgcccccgat gaggacgccg agcacgacca tgatcgccgg ttccatcagc gacgagagcg
     4021 cctcgaccgc gtcgtctacc tcttgctcgt agaagtcggc gaccttgccg agcatggcgt
     4081 cgagcgatcc ggtctcctcg ccgatcgaca ccatctggac caccatgttt ggaaacacgt
     4141 tggagttctg catcgccatc gtcagcgacg tgccggcgga cacttcctgc ttgatctgcc
     4201 gggtggcctg ggcatagatg tagttgcccg cggcgccgcc gaccgagtcg agcgcctcga
     4261 ccagcggcac gccggcggca aacatcgtcg acagcgtgcg cgtccagcgc gcgatggccg
     4321 atttcctcac caggtcgccg aaaagcggaa gccgcagcgt gaaccgatcc atgaagatct
     4381 gcaccgccac cgagcgcttc caggcttcca ggaagccgta gatcgcgccg ccgaggaccg
     4441 gaaatacgat gtaccagttg gcgacgaacc agtccgagat gccgatgacc acgagcgtcg
     4501 gcgcgggaag gtcggcgccg aagttggtga acacctcctt gaacgccggg atcacgaaga
     4561 tcatgatcac tgcggtgatg atgaacgcca ccgcgatgat cgcgatcgga tagaacatcg
     4621 cgctcttgat cttcgacttg atggcaagga tcttttcctt gtaggtcgca aggcggtcga
     4681 gcagggtctc gagaattccg gcctgctcgc cggcctggac caggttgcag aagagcgcgt
     4741 cgaagtgcag cgggaacttc cggaacgccg atgcgagcgc ggagccggtc tcgacgtcgg
     4801 tcttgatgtc ggtcagcagc ttcgccaccg ccgcgttcga ggcgcccttg ctcacgatgt
     4861 cgaaggattg cagcagcggc acgccggcct tcatcatggt tgcgagttgg cgcgtgaaaa
     4921 gcgcgatgtc cttctctgtg accttgccgc ccccgcgggt cttgaccggc tttaccttct
     4981 gcacaacgat gccctgacgg cgcaacgtcg cgttcaccgc ggcttcgctc actgcgcgca
     5041 gctcgccgcg aatcgtcttc ccggccttgt ccctgccttc ccagctgaag gtgaattgct
     5101 tcggccctct ggcggccgct gctgttgcca tactgagcgc tccttccccc ttgcttattc
     5161 ctttcgcccg gatgatgccc gcgggtgaag cgtttgtaaa gcgtgaagtt cctcttctaa
     5221 gcctctgagg gctcggtatt ttccacattc ttcacgggtc ggaataggcg caccgttttg
     5281 atcgtccggc cctgagcatg gacgatctcc atcggcacgg tcccgatctt gatcgaaacg
     5341 tccgcctcgg ggatgtcctg caggtattcg aggatcagcc cgttgagggt cttgggtccg
     5401 tcggtcggca gggagagcgc gagcgcgcgg ttcacctcgc ggaccggggt tgcgccctcg
     5461 gccgtagcgg cgccgctgcg gtcccaggcg agcgcctgcc ccgccgtcgg cagcgaggtg
     5521 gtgaacttgc cgatgatctc ctcgatgatg tcctcgagcg tgagtagtcc catgagttcg
     5581 ccgtactcat cgaccacgag cgcgatgcgc tcgcggttct cctggaagta ctgcatttgc
     5641 gcaagcaccg gggtggtcgc cggaacgaaa taaggctcgc taagcaccga ctcgagcgtc
     5701 ggcttgtcga gcgtcccggt cggcagcgcc ggcagcacct tcttcaggtg gagcacgccg
     5761 accacgtcgc cgagggcgct tcgaaacacc ggcagccggc catggtgact ggtgacgatc
     5821 tggcgcacga tcgtctccat gtcgtcgtcg aggctgagcg actcgatctt ggcgcgcggc
     5881 accatgatgt cctgcaccgt gactgcgccg acgtcgaaca ggttgagcag gatcgagacg
     5941 tgtttcttcg gcagcacgtt ggagtactcg agcaggatgg tgcggatttc ctccggcgac
     6001 atttcgcctt cgtcctcgcc gccgcgcatg cgcgcaagcg cgagcaacgg attgaggatc
     6061 gcattggtcg atgtcgagag cagggccacg aacggcgccg acatcttcgc cagcaggcgc
     6121 atcggccagg ccaccaagcg cgctatgcct tccgcgttat gctgcccgag gcgcttgggc
     6181 acgagctcgc cgacaacgat cgacacgtag gtaatcgtca ccacgatgcc gacggtcgaa
     6241 actacgttgg ccgtcttcgc tgtgatcggc agcgtggact gcagccaggc cgagaccggc
     6301 gcggacagga acgcttcgcc cacgatgccg ttcaggatgc caatcgaggt aatgccgatc
     6361 tggatcgtcg acaggaagcg ggtcgggtcg cgcgccaacg cgagcgccga ggcggcgagc
     6421 ttgtccccct gcttggctct cgtttcgagg cgaggcttgc gcgcggtgag cagcgccact
     6481 tccgacatgg cgagcgcgcc gttcaagaga atgaggctgg cgaggaggag gagttccatg
     6541 gattactcca ttcagctgcg tccgagcagc acttcgagca cgaagcggct gccgacgtag
     6601 gcgaggagca gcatgacgaa gccggtgagc gtccaacgca gcgcggtgcg cccgcgccag
     6661 ccgtagcgcc agcgcccggc aagcagcagg ccgaaagt
//
LOCUS       DASTPD010000024         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1641476, whole genome shotgun sequence.
ACCESSION   DASTPD010000024 DASTPD010000000
VERSION     DASTPD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641476"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFR57_01090"
     CDS             <1..984
                     /locus_tag="VFR57_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0258003.1"
                     /translation="AFTFLSDKLRAEYGVRRVHFAARPRESFIELDLGWQGAIVASEA
                     LSLWETQPLGEGPLTLRDVLERHGAEVWLHAAGPGGQRSGAFRFLLPAGEPAAAPKPP
                     ARAAESRPEYYDFDLFRYGDSARELHERRLSDLAYTVFDTETTGLEPSAGDEIISIGA
                     VRIVNARLLRNEVFERLVNPRRPLNRESVRIHGIDAAALAAEPGIEEVLPAFHRFCED
                     TVLVAHNAAFDMRFLELKEASTGVRFTQPVLDTLLLSAVLHPSLDDHRLETMADRLGV
                     QVIGRHTALGDALLEAEIFLRLLPLLAARGIATLRQALEASRQTYYSRLRY"
     gene            987..2408
                     /locus_tag="VFR57_01095"
     CDS             987..2408
                     /locus_tag="VFR57_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEU0258004.1"
                     /translation="MSKSTARLEQRIAEEQQAFARPLRSLVKRPALTCAPGITVAEAA
                     ALMRGESVGSLVVVDAAGRAVGIVTNQDIVAALANKGSGNSVVEAMTPNPFSLPGHAL
                     AYEAAVAMIAQRIRHVLVTEDGRLTGVVSERDLFSLQRLGLGELAMEIRLAASIEVLS
                     GLAGEVRALTRALVEQGVGAEQLTLFVSVLNDRISQRAIEIVRKRHDLERIRWCWLAF
                     GSEGRFEQTFSSDQDNGLLFETHDGGAPATARARLLPFAREVNEALDACGYPLCKGNV
                     MASNPELCLSLDEWKAKMARWIDVTLPKALLDAAICFDFRALYGDTTLSLALREWVAA
                     RVRAHPAFLRHLAEAALQGGPALGRFGGFATEEGMINLKLRGVKVFVDAARVYALAHG
                     LQQTNTAERLRAAAAHIGLRDVDAMADAFYVIQSLRLRVQKAASENRVAPDSLNRLER
                     ATLKEALRLGRELQERLGLDYQL"
     gene            2421..2729
                     /locus_tag="VFR57_01100"
     CDS             2421..2729
                     /locus_tag="VFR57_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258005.1"
                     /translation="MTKLYHLVALILRELVLINFYALLGQGLLFVLAGRHRETNLFYR
                     IIKTIASPAVWVARRISPRFVMDQHVPWVALFILVVLGVALTVAEQILRGQLTTQGPQ
                     "
     gene            2860..3126
                     /locus_tag="VFR57_01105"
     CDS             2860..3126
                     /locus_tag="VFR57_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEU0258006.1"
                     /translation="MQLTEKHKEYWRKNLTITAILLFLWFVVTFVIGYYARELHEITI
                     LGFPFAFYMGAQGSLIIYVIIIGVYAWYMNKQDKIYGVAEGEEQ"
     gene            3123..5216
                     /locus_tag="VFR57_01110"
     CDS             3123..5216
                     /locus_tag="VFR57_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEU0258007.1"
                     /translation="MTNGTAAYSQKAFTQSLKKYYSIYTGGFVAFVIVLAIAEQLGMP
                     NRWIGYAFLISTILLYAIIGVVSRTADIAEYYVAGRRVPALFNGMATGADWMSAASFI
                     GMAGSLYLLGYDGLAFILGWTGGYVLVALFLAPYLRKFGQFTIPDFLGARYGGHIPRT
                     IGLLCAILCSFTYVVAQIYGVGIITTRFTGVQLEVGVFLGLAGILVCSFLGGMRAVTW
                     TQVAQYIILIVAYMIPVVMLAYRNTGVPIPHVVYGYTLQKVTEREKQLTNDSKELQVR
                     EIFKKRAADADARLKALPASFNEEKAKVEKNLADLRASSAPPEKLKAAEEAVKNFPAS
                     EAEARIRWTAERAANTARAAPPRPHATEFPGATEKARDIARKNFLALIFCLMIGTAAL
                     PHILMRYYTTPSVRDARVSVTWSLFFIFLLYFTAPALAVQVKYEMYHAVVGTAFADLP
                     AWVKSWSAVDRTLLNIVDLNGDGLVQLAEISIGGDIVVLATPEIARLPYVISGLVAAG
                     GLAAALSTADGLLLTIANALSHDLYYKMINPQASTKTRVTVAKFLLLFVAIAAASVAS
                     QKPADILFLVSAAFSFAAASFFPALTLGIFWKRANKWGATLGMLAGTGVTFYYMATTQ
                     PWLRSVFGVTSSIVDNTWWDINPISAGIFGVPLGFAVIIIVSLLTPTPDQETQELVDH
                     VRFPVLEGDIDTRGT"
     gene            5291..5381
                     /locus_tag="VFR57_01115"
     tRNA            5291..5381
                     /locus_tag="VFR57_01115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5325..5327,aa:Ser,seq:gga)
     gene            5644..6102
                     /locus_tag="VFR57_01120"
     CDS             5644..6102
                     /locus_tag="VFR57_01120"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0258008.1"
                     /translation="MRFCKALVALAIFSLFAAPFAAHAAPTVSFKAPADGQTVSGSLR
                     QSGACEVAGSSINTSASVGSKTAAPYQCDIDTANFADGSCTLMAVATDKVGATSSTQR
                     PPTIERTSTPVAASDIIARASRANSARAPTHSLQQTRLQTAAGIGPRRRR"
BASE COUNT         1034 a         2082 c         2078 g         1153 t
ORIGIN      
        1 gcgttcacct tcctgtcgga caagctgcga gccgagtatg gagtacggcg ggtgcacttc
       61 gcggcgcggc cgcgggaaag cttcatcgag ctcgatctcg gctggcaggg cgcgatcgtt
      121 gccagcgagg cgctgtctct atgggaaacg cagccgttgg gcgaggggcc gcttaccctg
      181 cgcgatgttc ttgagcgcca cggggccgag gtctggcttc atgcggcggg acccggcgga
      241 cagcgcagtg gggcgttccg cttcctcctg ccggcgggcg agccggcagc cgcgccgaaa
      301 ccccccgcgc gcgcggccga gagccggccc gagtactacg acttcgacct ctttcgctac
      361 ggcgactcgg cgcgggagct gcacgagcgg cgcctctcgg atctcgccta cactgtcttc
      421 gacaccgaga ccacgggcct cgagccctcg gccggcgacg agatcatcag catcggagcg
      481 gtgcgcatag tgaacgcgcg cctgctcagg aacgaggtct tcgagcggct ggtcaatccg
      541 cggcgcccgc tgaaccgcga atcggtccgc atccacggca tcgatgctgc ggcgctcgcc
      601 gccgagccgg gcatcgaaga ggtgctgccg gcgttccatc gcttttgcga ggacacggtt
      661 ctggtcgcgc acaatgcggc tttcgacatg cgctttctcg agctcaagga ggcctcgacc
      721 ggcgtgcgct ttacgcagcc ggtgctcgac acgctgctgc tctcggcagt gctgcacccc
      781 agcctcgacg accaccgcct ggagacgatg gccgaccggc tgggggtgca ggtgatcggc
      841 cggcacaccg cgctcggcga cgccctgctt gaggcggaga tcttcctgcg cctgctgccg
      901 ctgctcgcgg cgcgcggcat cgcgacgctg cgccaggcgc tcgaagcctc acggcagacc
      961 tactactcgc ggctgcgcta ctagcgatgt caaagtccac cgcgcgcctg gaacaacgca
     1021 tcgccgaaga acagcaggcc ttcgcgcggc cgctgcgctc gctggtgaag cgtccggcgc
     1081 tcacctgcgc gcccgggatc acggtggccg aggcagcggc gctgatgcgc ggcgagagcg
     1141 tcggatcgct cgtcgtggtc gacgcagccg ggcgcgcggt cggcatcgtg accaaccagg
     1201 acatcgtggc cgcccttgcg aacaagggct ccgggaacag cgtcgtcgaa gcgatgactc
     1261 ccaatccgtt ctcgttgccg gggcacgccc tcgcgtacga agcggcggtg gcgatgatcg
     1321 cgcagcgcat ccgccacgtg ctcgtgaccg aggatgggcg gctcaccggc gtcgtgtccg
     1381 agcgcgatct gttctcgctg cagcgactgg gtttgggcga gcttgcgatg gagattcggc
     1441 tggcggcgag catcgaggtc ctttcgggtc ttgcgggcga ggtgcgcgcg ctgacgcgcg
     1501 cgctggtgga gcagggcgtg ggcgcggagc agctcacgct ctttgtttcg gtactgaacg
     1561 accgcatcag ccagcgcgcg atcgagatcg tgcgcaaacg ccacgacctc gagcgcatcc
     1621 gctggtgctg gctcgcgttc ggcagcgaag gccgcttcga gcaaacgttc agctccgacc
     1681 aggacaacgg gctgctgttc gagacgcacg acggcggagc gccggccacg gcacgcgcgc
     1741 gcctgctccc ctttgcgcgc gaggtgaacg aggcgctgga cgcctgcggc tacccgctat
     1801 gcaaaggcaa tgtcatggct tcgaaccccg agctctgcct ctcgctcgat gagtggaagg
     1861 ccaagatggc gcgctggatc gacgtcacgc tgccgaaggc gcttctcgac gcggcgatct
     1921 gcttcgactt tcgcgcgctg tacggcgaca ccacgctttc gttggcgctg cgcgaatggg
     1981 tcgcggcccg ggtgcgggca catcccgcgt tcctgcgcca cctcgccgag gcggcgctcc
     2041 agggcggccc cgcgctcggc cgctttggtg gcttcgccac cgaggagggc atgatcaacc
     2101 tgaagctccg aggcgtgaag gtgttcgtcg atgcggcccg ggtgtacgcg ctcgcgcacg
     2161 ggttgcagca aaccaacacc gccgagcggc tgcgcgcggc cgcggcccac atcggcctgc
     2221 gcgatgtcga tgccatggcg gacgcgttct acgtcatcca gagcctgcgg ctgcgcgtcc
     2281 agaaagcagc atccgagaac cgcgtcgccc cagacagcct caaccgtctg gaacgcgcta
     2341 ccctcaagga ggcgctgcgg ctcgggcgcg agctgcagga gcggctcggg ctcgactacc
     2401 agctctgagc ccggatggac atgaccaagc tttatcacct ggttgcgctg atcctgcgcg
     2461 agcttgtgct gatcaacttc tacgcgctcc tcggccaggg cctgctcttc gtgctcgcgg
     2521 ggcggcaccg cgagaccaac ctcttctacc gcatcatcaa gaccatcgcc tctccggcgg
     2581 tctgggtcgc gcgccgtatc tcgccccggt tcgtgatgga tcagcatgta ccctgggtgg
     2641 cgctcttcat cctcgtcgta ctgggtgtcg ccttgacggt cgcggagcag atcctgcgcg
     2701 gacagctcac gactcagggg ccgcaatgaa cgcgaaggtc cttgtgcgcc gcagcacgtg
     2761 cgccgggctg tcagccagcg taagcctgtg gtaagtattt gccgccacga tgcgatgggg
     2821 ctaaatcagg ggagtagttc cactacgagg aggagccaca tgcagctcac cgaaaagcac
     2881 aaagagtact ggcgcaagaa cctgacgatc acggcgatcc tgttgttcct atggttcgtc
     2941 gtcacgttcg tcatcggtta ctacgcgcgt gaattgcacg agataacgat cctcggcttc
     3001 ccgttcgcct tctatatggg cgcacagggc tcgctcatca tctacgtgat catcatcggc
     3061 gtgtacgcct ggtacatgaa caagcaggac aagatctacg gcgtggcgga aggagaagaa
     3121 caatgaccaa cggaaccgca gcctacagcc agaaggcttt cacgcagtcg ctgaagaagt
     3181 actacagtat ttacacgggc ggcttcgtcg cgtttgtcat tgtcctggcg atcgcggagc
     3241 aactcggcat gccgaaccgc tggatcggct atgcattcct gatttccacg atcctgctct
     3301 acgccatcat cggcgtggtc agccgcaccg cagacatcgc cgagtactac gttgctggac
     3361 ggcgcgtgcc ggccctgttc aacggcatgg cgaccggcgc cgactggatg agtgcggcct
     3421 ccttcatcgg catggcaggc tcgctctacc tgctgggcta cgatgggctc gccttcatcc
     3481 tgggctggac cggcggctac gtgctggtgg cgctgttcct cgctccgtac ctgcgcaagt
     3541 tcggccagtt cacgatcccc gacttcctcg gcgcgcgcta cggcggccac atccctcgca
     3601 ctatcggtct gctatgcgcc atcctctgct cgttcacgta cgtggtggcg cagatctacg
     3661 gcgtcggcat cattaccacg cggttcaccg gcgtgcagct cgaggtcggc gtgttcctcg
     3721 gcctcgcggg catcctggtg tgctcgttcc tcggcggcat gcgcgcagtg acgtggaccc
     3781 aggtcgcaca gtacatcatt ctcatcgtcg cctacatgat cccggtggta atgctcgcct
     3841 accgcaacac gggcgtgccg attccccatg tcgtgtatgg ctacacgctg cagaaggtca
     3901 ccgagcgcga gaagcagctc accaacgatt cgaaggagct gcaagtccgc gaaatcttca
     3961 agaagcgcgc ggctgacgcc gatgcgcgcc tgaaggcgct gccggcgtcg ttcaacgaag
     4021 agaaggcgaa ggtcgaaaag aacctcgccg acctgcgcgc ttcaagcgcg ccgccggaaa
     4081 agctcaaggc agccgaggag gcggtgaaga acttcccggc gtcggaagcc gaggcgcgca
     4141 tacgttggac ggccgagcgt gccgccaaca cggcgcgcgc ggcgccgccg cgtccgcatg
     4201 cgacggagtt cccgggcgcc acggagaagg cgcgggacat cgcccgcaag aacttcctcg
     4261 cgctcatctt ctgcctgatg atcgggaccg cggcgctgcc gcacatcctg atgcgttact
     4321 acaccacgcc gagcgtgcgc gacgcgcgag tgtcggtgac ctggtcgctg ttcttcatct
     4381 tcctgcttta tttcaccgcg ccggcgctcg cggtgcaggt gaagtacgag atgtaccacg
     4441 cggtcgtagg cacggcgttc gccgacctgc cggcctgggt caagtcgtgg tcggcggtcg
     4501 accgtacgct tctcaacatc gtcgacctga atggcgacgg cctggtgcag ctcgccgaga
     4561 tctcgatcgg gggcgacatc gtggtgctcg cgacgccgga gatcgcgcgc ttgccgtacg
     4621 tgatttccgg cctggtggcc gcgggcggtc tggcggcggc gctgtccact gccgacggac
     4681 tgctcctcac gatcgccaac gcgctgtcgc acgaccttta ttacaaaatg atcaatccac
     4741 aggcatcgac gaagacgcgc gtcacggtgg cgaagttcct gctgctcttc gtcgcgatcg
     4801 cggctgcgtc ggtcgcctcg cagaagccgg cggacatcct gttcctggtg agcgcggcgt
     4861 tctcgttcgc cgcggcgagc ttcttccctg cacttacgct ggggatcttc tggaagcgcg
     4921 ccaacaagtg gggcgcaacg ctcggcatgc tggcgggaac gggcgtcacg ttctactaca
     4981 tggcgaccac ccagccatgg ctccgctcgg tgtttggcgt gacgagctcc atcgtcgaca
     5041 acacctggtg ggacatcaac ccgatctcgg cggggatctt cggcgtgccg ctcggctttg
     5101 cggtgatcat catcgtgagc ctgcttaccc ccactcccga ccaggagacc caggagctcg
     5161 tcgaccacgt gcgcttcccg gtgctcgagg gcgacatcga cacccggggt acctgatctc
     5221 cgggcgcagg ccgaaaagcc cgccggggag gcgggctttt catttccgac ggttacaatg
     5281 tgcagctcgc ggagagatgg ccgagtggct taaggcgcac gcttggaaag cgtgtgtacg
     5341 ttaatagcgt accgtgggtt cgaatcccac tctctccgcc attagtactg gggggtccgt
     5401 tccggacacc ttaggtgacg ggccgtacct tagacatagg tgacaacctc gggccgaacg
     5461 ggttgtccag gggttgcagg gttttctgct ttgcgtgcgc ggcttgtcgt gtcgccccta
     5521 cagatatcta gtattccacc ctacgtccga ttgattcgat gtggcgaaat cccatgggca
     5581 tgggtcctgc tggcaggtcg cgcgagctgt ttcctttctt aataagtggg tagtttttct
     5641 ccaatgcggt tctgcaaggc actcgtcgct ttggcgatct tttcgctttt tgcggcgccg
     5701 ttcgcagcgc acgcagcgcc gacggtctcg ttcaaggctc cagccgacgg ccagaccgtt
     5761 tcaggcagcc tcaggcagtc gggtgcctgc gaagtcgccg gcagctcgat caacacgagc
     5821 gcgtcggtgg ggagcaagac cgcggcgccg taccagtgcg acatcgacac cgcgaacttc
     5881 gccgacggca gctgcacgct gatggcagtg gcgaccgaca aagtcggcgc gacctcgtcg
     5941 actcagcgcc cgcccaccat tgagaggacg tcaaccccgg tcgcagcgtc cgacatcatc
     6001 gcccgcgcga gccgcgccaa ctcggcgcga gctccaacgc actccctaca gcagactcgg
     6061 ctccagactg cggccggcat cggcccgcgc cgacggaggt agatgctgcc agaacgtcgg
     6121 ggagacggac cgcggacgcg cgccgatgag gccgcgctga tcgtgccgcg ggcattctta
     6181 agcgcgactt aagcccaatt catttaagac tgctggcggg gagatggaag acgccgcttt
     6241 ttcggatgag ttttttcgct ttctgcgcgc ggccgtgccc acagtggacg cggcggagct
     6301 gctgctgctc ctgcacggtg agccgcagcg ctggtgggcg gcgcagg
//
LOCUS       DASTPD010000025         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_8981, whole genome shotgun sequence.
ACCESSION   DASTPD010000025 DASTPD010000000
VERSION     DASTPD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8981"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFR57_01125"
     CDS             complement(<1..1380)
                     /locus_tag="VFR57_01125"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEU0258009.1"
                     /translation="MSTPPILWQPSERAIEEAQVTQFARQVVRKHRLDLNGYHDFHRW
                     TVENPELFWSEVWDWCGVIASRKGAPVLVDGDKMPGARWFPEARLNLAENLLRRGDRG
                     DALVFWDESGAKRRLSYSDLTSDVSRGAQALQAIGLRAGDRASAFIPNLPETSMLALA
                     ALSQGVVWSSCSPDFGVDGVLERFGQIEPKVLFCADGYRYNGQTHDSLERVRAIVERL
                     PTLRKVVVVPYLAPDVDVSDVPKAVRLDEWLRRYQPGDIAYAQLPFNHPAFVLFTSGT
                     TGKPKCLVHGAGGSLLQSLKTFKLHFDVRPGERFFYFCTTNWVVWNILFTGLAAEAAL
                     MLYDGSPFARNARVLFDYAEKERFTHFGTSAKFIDALSKRGLRPIDTHDLSSLKMIIS
                     TGSPLAPESFDYVYSSLKKDVCLSSISGGTDIMGAFVDASAVLPVYRGELQCRSLGMA
                     VEVFDEDGNS"
     gene            1436..>2092
                     /locus_tag="VFR57_01130"
     CDS             1436..>2092
                     /locus_tag="VFR57_01130"
                     /inference="COORDINATES: protein motif:HMM:NF026200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU0258010.1"
                     /translation="MRQAICRQHRVPEGEAVARLRQFQPDDATSRRIGETALRLARGV
                     RAAPPGALSAESFLRAYGLTTREGVALMCVAEALLRIPDADTQDALIRDKLASGSWSS
                     ASAADWALMLTGTLARWHGAEKESFSTLNQLIARLGEPVVRTAVRQAMRILAGQFVLA
                     ESIEEAVARAAERKPYRFSFDMLGEAARTAQDAERYFLSYKNAIAAVRAPDAVSIKLS
                     A"
BASE COUNT          384 a          696 c          681 g          331 t
ORIGIN      
        1 tgagtttcca tcttcatcaa agacctcaac ggccatgccg agggaacggc actgcagctc
       61 gccgcggtac accggaagca ccgcgctcgc gtccacgaaa gcgcccatga tgtccgtgcc
      121 gccggaaatc gacgacaggc acacgtcttt cttcagggag gaataaacgt aatcgaagct
      181 ttccggcgca agcggcgagc cggtcgagat aatcatcttc aaggacgaca gatcgtgcgt
      241 atcgatcggc cgcaggccgc gcttggaaag cgcgtcgatg aacttcgccg aggtgccgaa
      301 gtgcgtgaaa cgctccttct ccgcgtaatc gaacaggacc ctcgcgttgc gcgcgaaggg
      361 cgagccgtcg tagagcatca gcgccgcctc ggcagcgagg ccggtgaaca agatattcca
      421 caccacccag ttggtggtgc agaagtagaa gaagcgctcg cccgggcgca cgtcgaagtg
      481 cagcttgaag gtcttcagcg actgaagcag cgagccgccc gcgccgtgca ctaggcattt
      541 cggcttgccg gtcgtcccgg acgtgaacag cacgaacgca gggtggttga acggcagctg
      601 cgcgtaggcg atgtcgcccg gctgatagcg ccgcagccat tcgtccagcc gcaccgcctt
      661 tggcacgtcc gacacatcca cgtcgggggc gaggtacgga acgacgacga ctttgcgcag
      721 cgtcggcagg cgctcgacga tcgcgcgcac ccgctccagc gagtcgtgcg tctgcccgtt
      781 gtagcggtag ccgtcggcac agaaaagcac tttgggctcg atctgaccga agcgctcgag
      841 cacgccgtcg acgccgaaat cgggcgagca cgaggaccag acgacgccct gcgagagcgc
      901 ggcgagcgcc agcatgctgg tttcgggaag gttcgggatg aaagccgagg cgcggtcgcc
      961 agcgcgcagg ccgatcgcct gcagcgcctg cgccccgcgc gatacatcgc tcgtcaggtc
     1021 cgagtacgag agccgccgct tggcgccgga ttcgtcccag aacacgagcg catcgccccg
     1081 gtccccgcgc cgcagcaggt tttcggccaa gttaagccgc gcttcgggaa accagcgtgc
     1141 accgggcatc ttgtcgccgt cgacaagcac cggggctccc ttgcgggagg cgatcacgcc
     1201 gcaccaatcc cagacctcgc tccagaaaag ttccgggttc tcgaccgtcc agcggtggaa
     1261 atcgtgatag ccgttgaggt ccagccggtg cttgcggacg acctgccgcg cgaactgcgt
     1321 gacctgggct tcctcgatcg cacgctcgga aggctgccag aggatcggcg gtgtcgacat
     1381 atcggccgta cggtttttcg cggattatag tgagatgcgt caagcgattc gtaagatgcg
     1441 ccaagcaatt tgccgccagc accgcgtgcc ggaaggggag gcggtggcgc gcctgcgcca
     1501 gtttcagccc gacgacgcga cttcacgccg aatcggggaa accgcccttc ggctggcgcg
     1561 cggcgtgcgc gctgccccgc ccggcgcgct ctccgccgag tccttcctgc gcgcctatgg
     1621 gctcaccacg cgcgaaggcg tggcccttat gtgcgttgcc gaagcactgt tgcgcatccc
     1681 cgacgccgac acgcaggacg ccttgatccg cgacaagctt gcctcgggaa gctggagctc
     1741 cgcgagcgcc gccgactggg cgctgatgct caccgggacg ctcgcgcgct ggcacggcgc
     1801 agagaaggaa agcttctcga ccctcaacca gctcatcgcg cgcctcggcg agccagtggt
     1861 gcgcaccgcg gtgcgccagg cgatgcgcat cctcgccggg cagttcgtcc ttgccgagtc
     1921 gatcgaggag gcggtcgcgc gcgccgcaga acgcaaaccc taccgtttct ccttcgacat
     1981 gctaggcgag gcggcgcgca ccgcgcagga tgccgagcga tattttctgt cgtacaagaa
     2041 cgcaatcgct gctgtcagag ctcccgacgc ggtctcgatc aagctctcgg cg
//
LOCUS       DASTPD010000026         9202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_825578, whole genome shotgun sequence.
ACCESSION   DASTPD010000026 DASTPD010000000
VERSION     DASTPD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825578"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFR57_01135"
     CDS             complement(<1..267)
                     /locus_tag="VFR57_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258011.1"
                     /translation="MRLLIATFVAFVAVVVFAAPVGAQAQRGTVFRVGVLYPGGSEAL
                     SPRLAAFRQGLRTSGFVEGTNMSIEARSADGKSDRLASMAGELVK"
     gene            complement(395..1009)
                     /locus_tag="VFR57_01140"
     CDS             complement(395..1009)
                     /locus_tag="VFR57_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEU0258012.1"
                     /translation="MTFEIYIAYIVACFVIALVPGPTVTVIVANSLAHGSRAGLLNVA
                     GTQLGLALMMAILVVGLSSVIAAMGWLFDWLRWAGAAYLVWLGWKLLRAPEAMVDIQK
                     SSVPRGGFLLQGFLVLMANPKALLWFGAFIPQFIDPTGNYVGQIVVLGVTAMAVALVS
                     DGAYAVVTGRASAMLSRKRVRLVSRLSGGFLIGGGIWLALTRGR"
     gene            complement(1013..1651)
                     /locus_tag="VFR57_01145"
     CDS             complement(1013..1651)
                     /locus_tag="VFR57_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEU0258013.1"
                     /translation="MRLLLLLFFVATPAFAQYKGPAVEVCRAYAKREIARDGTRAKDV
                     IFEQDQELTLERYTRNVGSQFVSSILTGNGSVVIDGAPSAELSFVCLLADDKRAVFFE
                     WMPRSNPSAFKQCSRDATRIRECLQILLQVAETDLTQSYASPFLDARERDQGTGKEVF
                     ADAFRKANDEWKQYRDAECARRRDLTPQGVPADDWQLACVVDLTRRRLLDMR"
     gene            complement(1648..2619)
                     /locus_tag="VFR57_01150"
     CDS             complement(1648..2619)
                     /locus_tag="VFR57_01150"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0258014.1"
                     /translation="MKICVYGAGAIGGLMAAWLSRAGHEVSVIARGAQLEAIQREGLR
                     VRSKGSTESFRVRAESDATKLGAQDYVLVTVKAQSLPGVAESIAPLLGKETSVMTAMN
                     GVPWWFFHGLKGPDARLESLDPGARLSSAISTERIVGCVIHLAASTPEPGVVSHNMGL
                     KLIVGEPGGRNSARTRRIAEALTTAGFEVVVTQAIEKEFWVKLLGNVSFNPVSALTVT
                     TADQLIANQEVKAYMVEIMREVLAIGRAVGVDADIDPEARIDMARALGRFKTSMLQDL
                     EAGKPLEIDGLLAGTLEIARKAGVRAPYTESLYGLIRARAQSTGQYR"
     gene            2637..3293
                     /gene="msrA"
                     /locus_tag="VFR57_01155"
     CDS             2637..3293
                     /gene="msrA"
                     /locus_tag="VFR57_01155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0258015.1"
                     /translation="MFTFMKKMKALLPKESTLPGRAESMRVPAKHFVNGHALKPPFPE
                     GMQLAQFGMGCFWGAERVLWETPGVYSTAVGYSGGETKNPSYEEVCSGFTNHAETVLV
                     VFDPKVVTYEELLKRFWESHDPTQGMRQGNDAGTQYRSTIYTYGDAQMKAALASKAEF
                     QKALSKAGFGPITTEIRPAPEFYYAEEYHQQYLAKNPGGYCGLGGTGVSCPAGIAVSG
                     "
     gene            3406..4287
                     /locus_tag="VFR57_01160"
     CDS             3406..4287
                     /locus_tag="VFR57_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258016.1"
                     /translation="MLAPRPLAESFYTGLIVQRLRELGYREGSGMVLEYRATRAVPQR
                     YPEMAHELVALKCDLIFAVGPDNGVIALRDARTPIPVVFLAIDFDPLQSGIVTSLGRP
                     GSNITGVYVPQGELAAKRLDILREVLPSAKRFLIFSDEFTRDQLATLRRRADSIGAQL
                     VIAEFSRQPYDYAGAFDTARREKAEALVGLASAVFAADGAKIGALLAEARLPSAGTSA
                     AMAERGFMLSYGPDNSKITRRVAEIAVRILKGAKPAEIPVEQADEFTLVVNGPVASKL
                     GLRIPESVMARATRIIA"
     gene            4284..4661
                     /locus_tag="VFR57_01165"
     CDS             4284..4661
                     /locus_tag="VFR57_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU0258017.1"
                     /translation="MRVDKWLWAARFFKTRSLAQQAIAAGRVKLNDARVKSSHELKAG
                     DSLAVRVSDYEWRIVVKDLSDKRGPAEQARKLYEETAQSRAERERRMDLRRWGAEPAS
                     TLKGRPTKRDRRVIEDFTSPEDS"
     gene            4700..6733
                     /locus_tag="VFR57_01170"
     CDS             4700..6733
                     /locus_tag="VFR57_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0258018.1"
                     /translation="MGGVLLLGAAAAAAQEDDTVVITATRAPRPSLETPASVDRLYAE
                     EIREGRPQVNLSESMGRVPGIVVQDRGNYAQDFRVQSRGFGARAAFGVRGIRLIADGI
                     PATMPDGQGQGATFALGSAERIEVLRGPFSSLYGNAAGGVIVVDTEDPPAEPTIGGDV
                     FAGRYGTWRAGVKLGGFNTLLDASRFETDGYRDHSAARRDHYNLKFKYPLARDTSLTI
                     VGNQLRQPQTQDPLGLTRAQFEENPRQVTPQAITFDTRKTVYQEQLGATLTHRLGGTS
                     VLQATAYGGQRWVEQYLAIPLAVQAAPTHSGGVIQLDRNYGGAALRLSTGDVVRLAAG
                     VEYETMAERRKGFINNNGTAGALKRDEDDTVSSTDLYAQVEWRFAERWSAHGGLRASR
                     VSFESEDFFIVPGNPNDSGSRDYSETTPVAGIVFRPTRTVTLYGTYGEGFETPTFVEL
                     AHSSAGSGLNFGLDAARSRHLEAGVKAVLPRRARLSAALFDVLTKDEIVVETSSGGRT
                     TFKNAGRTERRGVELGLDTISPGPWGAALAYTYLEATYRDSFATVAGSPAAPVTVPAG
                     NVLPAVPESQLYGQLRYRQPRYYGYLEMLRRSSIAVDDLNSEFAEGYTLFNLVAGLVQ
                     QGPGWRVSEYVRVDNLTDRNYVGSVIVNEANRRFYEPAPRRSIGAGIQATIQF"
     gene            6762..7994
                     /locus_tag="VFR57_01175"
     CDS             6762..7994
                     /locus_tag="VFR57_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0258019.1"
                     /translation="MRSLIALAVAAAFAAPALPQDKPKPPPAWHQGKPAAMAESKLAP
                     HAGKMTETPASDIPISKLKLPAGFKAEIWATGLPGGRAMAVSDDGKKVYVGTRVIGRV
                     YEVSDEGSKRSVRVVADKLTQPAGVALKDGNLYVFAIDKVLRFDGITKNADAKPEDMT
                     AKFDLPPEQHHNWKYIAFGPDGKLYVPFGAPCNICEPPSKEYAQIRRYNADGSGKEVI
                     ATGVRNSVGFDWHPKTKELWFTDHGRDWAGDKGFEDELNKISKTGQNFGFPYCHANGQ
                     PDPDVKKAKACEGVTRPVLLMGPHAAAMGIKFYTGKMFPSEYQNTAFVVRKGSWNREK
                     PFGFDVVNVRVGEDGKASIRNFASGWQEGGEGYKFWGRPAYVAQMPDGALLVSDEQVG
                     AIYRISYQRPAKAPAKKQ"
     gene            7994..8575
                     /locus_tag="VFR57_01180"
     CDS             7994..8575
                     /locus_tag="VFR57_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0258020.1"
                     /translation="MRSWKGRWALLTAFFISIACGQDRVQLCAACHGDDGNSTNPQIP
                     SIAGQPKLFLENQLILFREELRKSEQMAPVVKGMKDPEIIKLAEHFSKLPAKVMESAP
                     ADPALVKQGTAKAKQLRCGVCHLADFSGQNQIPRLAGQRETYLDAEMRAYRDGKRTGG
                     DTIMAAALYGVSDADVKALAHYLSRSTSRSPAR"
     gene            complement(8520..8966)
                     /locus_tag="VFR57_01185"
     CDS             complement(8520..8966)
                     /locus_tag="VFR57_01185"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEU0258021.1"
                     /translation="MATIWIIAADESRARVLQVLDREQRLAEIEDLVNPAGRAQDREL
                     QTDAEPRFNGSQGGPASDPERQGAVEHSVRTFAKEVGRYLDRARNEHRYDQLVLVAPP
                     KFLGALRKELHKEVEKLVTDELPKDLSWFNAREIEKYFAKGSGRAP"
     gene            9000..>9202
                     /locus_tag="VFR57_01190"
     CDS             9000..>9202
                     /locus_tag="VFR57_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU0258022.1"
                     /translation="MNPLFIGGALGALAMYFLDPNLGRRRRARTRDKVTHAAKVVNEG
                     ARVTARDTVQRAQGVWAEAKRMFA"
BASE COUNT         1717 a         2981 c         2988 g         1516 t
ORIGIN      
        1 cttaaccaat tcgccagcca tgcttgcgag ccggtcgctt ttcccatccg cagagcgcgc
       61 ctcgatcgac atgttggtgc cctcgacgaa gccggaggtc cgcaggcctt gccggaatgc
      121 cgccagtcga ggtgatagag cctcagaccc gccggggtag agcactccga cacgaaagac
      181 tgtcccgcgc tgtgcctggg cgccgaccgg cgctgcgaaa acaacaacag ccacaaacgc
      241 cacgaatgtg gcaatcagga gacgcatacc ccctttgaca gcgactctat tatcacaata
      301 agtcagcgct aggcgcttcg ccattctcgg cgatagggct caggactgcc gaaggacaat
      361 ctcagttgaa ctcatggcgc cgcgcatgga aggttcatcg ccctcgcgta agcgcgagcc
      421 aaatgcctcc gccgatgagg aagccgccgg agagccgcga gaccaggcgc acacgttttc
      481 tggagagcat agcgctggcg cggccggtca cgacggcata tgcaccatcc gacactagcg
      541 cgactgccat cgctgtgaca ccgagaacaa cgatctggcc gacgtagttg ccggtcgggt
      601 cgatgaactg cgggatgaaa gcgccgaacc aaagcagcgc tttggggttt gccatgagga
      661 cgaggaagcc ttgcagcaga aatccgcctc ttggcaccga cgacttctga atgtctacca
      721 tggcctccgg cgcacggagc agtttccatc ccagccagac gagatacgcg gcgccggccc
      781 agcgcagcca gtcgaagagc cagcccatgg cggcgatcac cgagctcaag cccacgacca
      841 ggatcgccat catcagcgca agcccgagct gcgttcccgc gacgttgagg aggcctgcac
      901 gcgagccgtg cgcgaggctg ttcgcgacga tgaccgttac cgtcggcccc ggaacaagcg
      961 cgatgacgaa gcaggcgacg atgtaggcga tatagatctc gaaagtcacg gactacctca
     1021 tgtccagcaa acgccgccgg gtgaggtcga cgacacaggc gagctgccaa tcgtcggccg
     1081 ggacgccttg gggcgtgaga tcgcgccgtc gcgcgcattc ggcgtcgcga tactgcttcc
     1141 attcgtcatt cgccttacgg aacgcatcgg cgaagacctc cttgccggtt ccctggtcgc
     1201 gctcgcgagc atcgaggaac ggggaggcgt agctttgcgt caggtcggtc tccgcaacct
     1261 gcagcaaaat ctgcaggcat tcacgaattc gcgtggcgtc gcggctgcac tgcttgaacg
     1321 ccgatggatt ggagcgcggc atccattcga agaagacggc gcgcttgtcg tccgcaagca
     1381 ggcagacaaa ggagagctcg gcgcttgggg cgccgtcgat gactacggaa ccatttccgg
     1441 tgaggatcga ggaaacgaac tggctgccga catttcgcgt atagcgctcc agcgtcagct
     1501 cctggtcctg ctcgaagatc acgtctttcg cgcgcgtgcc gtcgcgcgcg atctcgcgtt
     1561 tcgcatacgc gcggcacacc tccaccgccg gacccttgta ttgcgcgaac gccggtgtag
     1621 cgacgaagaa cagaagcaga agaagcctca tcgatactgt ccagtcgatt gcgcgcgcgc
     1681 gcgaatcagg ccatacaggc tttcggtgta gggcgcccgc acgccggctt tgcgcgcgat
     1741 ctcgagcgtg ccggcaagaa ggccatcgat ctcgagcggc ttgcccgctt ccagatcctg
     1801 gagcatcgaa gtcttgaagc gcccaagggc gcgcgccatg tcgatgcgag cttccggatc
     1861 aatgtcggca tcgaccccga cggcacgccc gatggcgagc acctcgcgca tgatctcgac
     1921 catgtaagcc ttgacctcct ggttcgcgat gagctgatcc gccgtcgtca ccgtcaacgc
     1981 ggacaccggg ttaaagctca cgttgccgag gagcttcacc cagaactcct tttcgattgc
     2041 ctgcgtcacc accacctcga agccggccgt ggtcagcgcc tccgcaatgc gccgcgtgcg
     2101 cgcgctgttg cgtccgccgg gctcacccac gatgagcttc aggcccatgt tgtggctgac
     2161 tacgcccggc tcgggcgtgg aggcggcgag gtggattacg caacctacga tgcgctcggt
     2221 cgatatggcg ctcgacagtc gtgcgccggg atcgagcgat tccaggcgtg catccgggcc
     2281 tttcaggcca tgaaagaacc accacggcac gccgttcatg gcggtcatga ccgacgtttc
     2341 cttgccaagg agcggagcga tactctcggc gacgccgggc aggctctgcg ccttgacggt
     2401 gacgaggacg taatcctgtg cgcccagctt cgtcgcgtcg ctttccgcgc gcacgcgaaa
     2461 ggactccgtg ctgcccttgc tgcgcactcg aaggccctcg cgctggatcg cttccagctg
     2521 ggcgccgcgc gctatcaccg agacctcgtg gccggcgcgg gatagccaag cggccatcag
     2581 gccgccgatc gctccggcac cgtagacgca gattttcacg gtgctattct agggtgatgt
     2641 tcacattcat gaagaagatg aaggcgctcc tgcccaagga gagcacgctt cctgggcgcg
     2701 ccgaatccat gcgcgtgccc gcaaaacatt tcgtgaacgg gcacgcgctc aagccgccgt
     2761 tccccgaagg catgcagctc gcgcagttcg gcatgggctg cttctggggc gccgagcgcg
     2821 tgctctggga aacacccggg gtctactcga ccgccgtcgg atattccggc ggcgagacga
     2881 agaatccgag ctacgaagag gtctgtagcg gcttcacgaa tcatgccgaa acggtgctcg
     2941 tcgttttcga tccgaaggtc gtgacgtacg aagaactcct caagcgcttc tgggagtcgc
     3001 acgatcccac gcaaggcatg cgtcagggca acgacgcggg cacgcaatac cgctcgacga
     3061 tctacaccta tggtgatgcg cagatgaagg cggcgctcgc ctcgaaagcc gagttccaga
     3121 aggcgctcag caaggccggc ttcggcccga tcacgaccga gatccgtccg gcgccggagt
     3181 tctactatgc cgaggaatac caccagcaat acctcgccaa gaatcccggc ggctattgcg
     3241 gcctcggggg gacgggggtc tcctgtcctg ccggaatagc agtcagcggc tgaaccgaag
     3301 tagatcgctg gcgcgatgac gtcccgtcgg caagcgatcc tcgcgcttct cgttgggact
     3361 gccgtcccag gcattcgcgc acagggccgg gccgtaagga tcggcatgct cgccccgcgc
     3421 ccgctcgccg agtcgtttta tacggggctt atcgttcagc ggcttcgcga gctgggatat
     3481 cgcgaagggt ccggcatggt gctcgagtat cgcgcgaccc gcgccgtccc tcagcggtat
     3541 ccggagatgg cgcacgagct cgtggcgctc aagtgtgatc tcatcttcgc ggtcgggccg
     3601 gacaatggcg tgatcgcatt gcgagacgcg cgcaccccca tcccagtggt atttctcgcc
     3661 atcgatttcg acccgcttca atccggtatc gtgaccagtc tcggccgccc gggctcaaac
     3721 atcaccggcg tctatgtccc gcagggggaa ctcgcggcga agcggctcga catcctgagg
     3781 gaagttctgc cgtcggcgaa gcgcttcctt atcttctcgg acgagttcac gcgagatcag
     3841 ctcgccacgc tgcgtcggcg cgccgattcg atcggtgcgc aactcgtcat cgcggagttc
     3901 tcccgccagc cctacgacta cgcaggcgca ttcgacaccg ctcgcaggga aaaggcagag
     3961 gccctggtcg ggctcgcttc ggcggtattc gctgcggacg gtgcgaagat cggcgcgctg
     4021 ctagccgaag cccgcttacc gagcgccggc acgtcggctg ctatggcgga gcgcggcttc
     4081 atgctgagct acgggccgga caattccaag atcacgaggc gcgtcgccga aatcgcggtg
     4141 cgcattctca agggcgcgaa gccggctgaa ataccggtgg agcaggccga cgagttcacg
     4201 cttgtcgtaa acgggccggt ggccagtaag ctcgggctaa gaatcccgga gtctgtgatg
     4261 gcgcgcgcga cgaggatcat tgcgtgaggg tggacaaatg gctttgggcg gcgcggtttt
     4321 tcaagacgcg cagcctagcg cagcaagcga tcgccgctgg tcgcgtaaag ctcaacgacg
     4381 cccgcgttaa gtcatcgcat gagctgaaag ctggtgattc cctcgccgtc cgggtcagcg
     4441 attacgagtg gcgcatcgtt gtgaaggacc tctcggacaa gcgcgggccc gcggagcagg
     4501 cgaggaagct ctacgaggaa accgcgcaga gccgcgccga gcgcgagcgg cgcatggatc
     4561 tacggcggtg gggcgcggag ccggcgagca cgctgaaggg gcggccgacg aagcgggatc
     4621 gccgcgtgat cgaggacttc acgagtcccg aggactccta gcattttcaa ggtagcctag
     4681 cggcccaatg cgaaggctca tggggggagt gttgctgctc ggcgctgccg cagcggctgc
     4741 gcaggaggac gacactgtcg tcatcacggc gacgcgcgcg ccgcggccga gcctggagac
     4801 tccggcatcg gtcgaccgcc tctatgccga ggagattcgc gaaggccggc cgcaggtcaa
     4861 tctgtcggag agcatgggcc gcgtacccgg catcgtggtg caggaccgcg gaaactacgc
     4921 gcaggatttt cgcgtgcagt cgcgcggctt cggtgcgcgc gctgcgttcg gcgtgcgcgg
     4981 aatccggctt atcgccgacg gcattcccgc aaccatgccg gatggccagg ggcagggggc
     5041 gacctttgcc ttgggctcgg ccgagcgcat cgaagtgctg cgcggaccgt tctccagcct
     5101 gtacggcaat gccgcgggcg gcgtcatcgt cgtcgacacg gaggatccgc ccgcggagcc
     5161 gacgatcggc ggcgatgtct tcgccgggcg ctacgggacc tggcgcgccg gggtcaagct
     5221 gggcggcttc aacacgctgc tcgatgcgtc gcggttcgaa accgacggct atcgcgacca
     5281 cagcgcggcg agacgggacc actacaacct caagttcaag tatccgcttg cgcgcgacac
     5341 ttcgctcacg atcgtcggta accagctgcg ccagccgcag acgcaggatc cccttggtct
     5401 cacgcgcgcc cagtttgagg agaacccacg gcaggttacg cctcaggcga tcaccttcga
     5461 tacgcgcaag acggtctatc aggagcagct cggcgctacg ctgacccatc gtctcggcgg
     5521 tacctcggtg cttcaggcga ccgcttacgg cggccagcgc tgggtggagc agtacctggc
     5581 gatcccgctt gcggtgcagg cggcgccgac gcactcgggc ggcgtcattc agctcgaccg
     5641 gaactacggc ggagccgcgt tgcgcctgtc gaccggcgat gtcgtgcgcc tagccgcggg
     5701 cgtcgaatac gagaccatgg cggagcgccg caagggtttc atcaacaaca acggcacggc
     5761 gggcgcgctc aagcgcgacg aagacgacac ggtatccagc accgacttgt acgcgcaggt
     5821 ggaatggcgc tttgccgagc gctggagcgc gcacggaggc ttgcgcgcga gccgcgtgtc
     5881 cttcgaatcc gaggatttct tcatcgtccc cggcaatccg aacgacagcg ggagccgcga
     5941 ctattcggaa acgacgcccg tggcggggat tgtgtttcgg cccactcgca ccgtcacgct
     6001 gtacggcaca tatggggagg gcttcgagac gccgacgttt gtcgagctgg cgcacagcag
     6061 cgcgggcagc gggctgaatt tcgggctcga cgccgcgcgc agccgccatc tggaagccgg
     6121 cgtcaaggcc gtcctgcccc ggcgtgcaag gctgagcgcc gcgcttttcg acgtgctcac
     6181 gaaggacgag attgtcgtcg aaacctcttc aggcggccgc accaccttca agaacgccgg
     6241 ccggacggag cgccgcggcg tcgaactcgg gctcgacacg atcagtcccg gtccttgggg
     6301 agcggcgctc gcttatacgt atctcgaggc aacttaccgc gactccttcg ccacagtagc
     6361 cggaagcccg gccgccccgg tcaccgtccc ggccggcaac gtgctcccgg cagtgccgga
     6421 gagccagctt tacggccagc tgcgctatcg gcagccgcgc tactacgggt acctcgagat
     6481 gctgcgccgc tcgagcatag cggtggacga tctcaactcc gaattcgccg agggctacac
     6541 gctattcaac ctggtggccg gcctggtgca gcaggggccg ggatggcgtg taagcgaata
     6601 cgtacgcgtc gataacctga ccgaccgcaa ttacgtggga tcggtgatcg tgaacgaagc
     6661 gaaccggcgg ttctacgagc cggcaccgcg gcgcagcata ggcgctggca tccaggcaac
     6721 cattcagttc taataacgat tcgccagagg agagaaaaac catgagatca cttattgcac
     6781 tcgcggtcgc tgccgcattc gcggcgcccg ccttgccaca ggacaaaccc aagcccccgc
     6841 ccgcgtggca tcaaggcaag ccggccgcga tggccgagtc gaagctcgct ccgcatgccg
     6901 gcaagatgac cgagacgccg gcaagcgaca tcccgatctc gaagctaaag ctcccggccg
     6961 gcttcaaggc cgagatctgg gcgaccgggc tgccgggcgg acgcgccatg gcagtcagcg
     7021 acgacggcaa gaaggtgtac gtcggcacgc gggtgattgg ccgggtctac gaggtgagcg
     7081 atgagggctc gaagcgcagc gttcgcgtgg tggccgacaa gctcactcag cctgcgggcg
     7141 tcgccctgaa ggacggcaac ctgtacgtgt tcgccatcga caaggtcctg cgcttcgacg
     7201 ggattaccaa gaacgccgac gcaaagccgg aggacatgac ggcgaagttc gacttgccac
     7261 ccgaacagca ccacaactgg aagtacatcg cgttcggacc cgacggcaag ctctacgtgc
     7321 cgttcggcgc gccctgcaac atctgcgagc cgcccagcaa ggagtacgcg cagattcgcc
     7381 gctataacgc cgacggctca ggcaaggaag tgatcgccac cggcgtgcgc aactccgtcg
     7441 gcttcgactg gcatccgaag accaaggagc tctggttcac cgatcacggt cgcgactggg
     7501 ccggcgacaa gggcttcgag gacgagctga acaagatcag caagaccggc cagaacttcg
     7561 gcttcccgta ctgccatgcc aacggccagc ccgatccgga cgtgaagaaa gcgaaagctt
     7621 gcgaaggcgt gacgcggccc gtgctcctga tggggccgca cgccgcggcg atgggcatca
     7681 agttctacac cggcaagatg ttcccgtcgg agtaccagaa caccgcgttc gtggtgcgca
     7741 agggctcgtg gaaccgcgaa aagccgttcg gcttcgacgt ggtcaacgtg cgcgtcggcg
     7801 aagacggcaa ggcaagcatc cgcaatttcg ccagcggctg gcaggaaggc ggcgagggct
     7861 acaagttctg gggtcgcccg gcttacgtgg cgcagatgcc cgacggcgcg ctgctcgtct
     7921 cggacgagca ggtcggcgca atctaccgca tcagctacca gcgtcccgcg aaggcgcccg
     7981 cgaagaaaca gtaatgcgct cgtggaaggg gcggtgggct ttgctcaccg ccttttttat
     8041 ttctatcgcc tgcggtcagg accgcgtaca gctgtgcgcc gcctgccacg gggacgacgg
     8101 caactccacc aacccgcaga tcccatccat cgccgggcag ccgaagctct tccttgagaa
     8161 ccagctcatc ctctttcgcg aggagctgcg caagtccgag cagatggcgc cggtcgtgaa
     8221 gggcatgaag gatcccgaga tcatcaagct ggccgagcac ttctcgaagc tgccggcgaa
     8281 ggtcatggaa agcgcgccgg cggatccggc gctcgtgaag caaggcactg caaaagcgaa
     8341 gcaactgcgc tgcggcgtct gccacctcgc agatttcagc ggccagaacc agatcccgcg
     8401 cctcgcaggt cagcgcgaga cgtacctcga cgcggagatg cgcgcctacc gcgacggcaa
     8461 gcgaacgggc ggcgacacga tcatggccgc cgccctgtat ggcgtaagcg acgcggacgt
     8521 caaggcgctc gcccactacc tttcgcgaag tacttctcga tctcccgcgc gttgaaccac
     8581 gagaggtcct tggggagctc gtcagtcacc agcttttcga cctccttgtg cagctccttg
     8641 cgcagcgcgc cgaggaactt gggtggcgcg accagcacga gctggtcgta ccggtgctcg
     8701 ttgcgcgccc ggtcgagata gcgcccgacc tccttggcga aggtccgcac gctgtgctcg
     8761 accgcgccct ggcgttcggg gtcgctcgcc ggaccacctt ggctgccgtt gaacctcggc
     8821 tcggcgtcgg tctggagctc gcggtcctgg gcccggcccg ccgggttgac caggtcttcg
     8881 atttcggcga ggcgctgttc ccggtccaga acttgcagca ctcgcgcgcg gctctcgtcg
     8941 gccgcaatga tccatatcgt agccatgaac gttcgaccgc gaatatcctc gaaaatctca
     9001 tgaaccccct attcatcggc ggcgcgctcg gggcgctggc catgtatttc ctcgatccga
     9061 acctcggccg gcgtcgccgg gcgcggacac gcgacaaggt cacgcatgcc gccaaggtgg
     9121 tgaacgaggg cgctcgcgtc accgcgcgcg acacggttca gcgcgcgcag ggtgtctggg
     9181 ccgaggcgaa gcgcatgttt gc
//
LOCUS       DASTPD010000027        23783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1234459, whole genome shotgun sequence.
ACCESSION   DASTPD010000027 DASTPD010000000
VERSION     DASTPD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234459"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFR57_01195"
     CDS             complement(<1..210)
                     /locus_tag="VFR57_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0258023.1"
                     /translation="MAKGKFERNKPHVNVGTIGHVDHGKTTLTAAMTHILAKKYGGEA
                     KNYDQIDAAPEEKARGITINTAHVEY"
     gene            complement(223..297)
                     /locus_tag="VFR57_01200"
     tRNA            complement(223..297)
                     /locus_tag="VFR57_01200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Thr,seq:ggt)
     gene            complement(332..405)
                     /locus_tag="VFR57_01205"
     tRNA            complement(332..405)
                     /locus_tag="VFR57_01205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(371..373),aa:Gly,seq:tcc)
     gene            complement(459..545)
                     /locus_tag="VFR57_01210"
     tRNA            complement(459..545)
                     /locus_tag="VFR57_01210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Tyr,seq:gta)
     gene            complement(594..1397)
                     /locus_tag="VFR57_01215"
     CDS             complement(594..1397)
                     /locus_tag="VFR57_01215"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEU0258024.1"
                     /translation="MRVALLGGGTIARLVLERRPPGIEFAALCGRGPASRGVALAREF
                     GVPYVSDRTQLLGARPEVVIEAASHDAVREHLVPLLDAGVGVVVLSAGALADDGLRAR
                     AEAAAQRTGALFYVPSGGIGGLDALKTACIGGVDEVSIQVAKPPQAWKGIAYVETLGL
                     DLERLKGATTLFEGPAREGVPHFPQNVNIAAVLSLAGLGMDRTRLKVVADPALTLNTH
                     TIRVSGACGRITVVLENVPAPQNPKTSWLACYSALAALEALRSRVRYGT"
     gene            1450..2142
                     /locus_tag="VFR57_01220"
     CDS             1450..2142
                     /locus_tag="VFR57_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258025.1"
                     /translation="MRIAILEDDPDQLALLRRWISEDGHDVHAYLAGREAMKQAGRES
                     FDLFMLDWQVPDVSGAEVLMWIRANVSKSVPVLFVTVRDSEQDIVFALEHGADDYMVK
                     PVRRQELLARVHALLRRAYPTEEKKQLSFPPFEIDTQRAEVRRDGVVIDLTPKEFELT
                     VTLFRNIGRLLSRGHLQESVWGRTGDLATRTVDTHVSQVRKKLDLRPESGYRVVPIYN
                     YGYRLEKISPAA"
     gene            2368..3357
                     /locus_tag="VFR57_01225"
     CDS             2368..3357
                     /locus_tag="VFR57_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0258026.1"
                     /translation="MPNALQELTIFAKVVGTGSLSAAARDLGMSPAVVSRRLASLEAR
                     LGVRLVNRTTRSLHLTDEGAAYYETCTRVLADIEEADAAVSAGRAEPRGVLRVALPAS
                     FGNQHVAPLVPKFAQLYPNVQIALSLSDRTVNLVEEGFDLAIRIADLADSALAARRLA
                     PNRRVVCASPAYLRRNGTPATPEDLAKHNCLTTTDFAMNWDYRAPDGKAGSVRVAGRY
                     ACDNGEVLREWALAGLGIALKSTWDVRRHLEDGSLVSLLPGYTFASDVAIYAVYPHRR
                     HLPAKTRAFIEFLADSFGPEPYWDREKSASDERKSGSDPDFPPKPRIKGRSPV"
     gene            3365..6826
                     /locus_tag="VFR57_01230"
     CDS             3365..6826
                     /locus_tag="VFR57_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEU0258027.1"
                     /translation="MFSLEDKYTLANGTVFLTGMQALARLMMLQRERDLGAGLNTAGF
                     ISGYRGSPLGGFDQTLARARAHLEKSHVRFQPGLNEDLAATAIWGTQQLNLFPGANYD
                     GVFSMWYGKGPGVDRCGDVFKHANAAGTSKHGGVLVLAGDDHGAKSSTLPHQSEHIFK
                     ACLIPVLNPANVQDYLDLGLHGFAMSRYSGCWVAFKCVTDIVESGASVVVDANRVAIR
                     TPGDFVLPPDGLNIRWPDGILEQEARILDWKVYAALAYVRANGLDRIVYDSPKARLGI
                     VTTGKSYGDVMQALNDLGITKDVARDIGLRVYKVAMSWPLEPQGARRFADGLEEILVV
                     EEKRQLIEYQIKEELYNWKEGVRAPRVVGKFDDSGEWSRSGGRPAGTWLLPAHYEHSP
                     AMVAKAIAQRLEKLGMANRLGSQFRERLAFLDFKEKILKRPRVTAVRQPYFCSGCPHN
                     TSTRVPEGSRATAGIGCHFMAVWMNRSTATFTHMGAEGAPWIGQAPFTKEKHIFANLG
                     DGTYFHSGILAIRAAVAAKVRMTYKILYNDAVAMTGGQRHDGPLDPPTISRQLAAEGV
                     APIVVVTDEPTKYPAATAWAKGVTIRHRDDLDAVQRELRETKGVSALIYDQTCASEKR
                     RRRKRDEYPDPAKRVLINEMVCEGCGDCSVKSNCLSVEPLETEFGRKRQINQSSCNKD
                     YSCVKGFCPSFVTIEGGRLRKGKAAPKETEGEVPSPQIPSLAAPYGILVTGIGGTGVI
                     TIGQIIGVAAHVEGKGVTVLDMSGLAQKYGAVMSHVQVAASPDQMHATRIDTGGANLV
                     LGCDLVVTASTESLAKMAPKRTRALVNASVTPTAEFVRNPNWQLPGTDLTKDVAESCE
                     KAEFVEATAIATGLMGDAIATNMFMLGYAYQKGWIPLAAESLERAIELNGVAVEFNRK
                     SFVWGRRAALDLEKVRRIAMPAEVIPIEQHFSRNLDELIERRVRLLTEYQNRAYADRY
                     RALVEKVRAKDEKLAEIVARNYAKLLAYKDEYEVARLHADANFRKKIEGMFEGDYRVV
                     YHLAPPLLTRKEEKVRFGPWMLPVFKVLRNFKFLRGSALDPFGYTQERRTERALISEY
                     EDTVARLLARLNSQNEALALEIAAIPDEIRGFGHIKARNLAPARKKWQELLARYEAGQ
                     PAQRAAA"
     gene            6960..7298
                     /locus_tag="VFR57_01235"
     CDS             6960..7298
                     /locus_tag="VFR57_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258028.1"
                     /translation="MKVLRLLADGDIETAAKTSNAPERRLEVLRDYRKRVGDEEFKRA
                     FARYFETPVVAEVALGPRRLLVWNLGEDLAAQYYIEREGRFVMDDVPSPARAELARIL
                     REFRSGHLKY"
     gene            complement(7288..8016)
                     /locus_tag="VFR57_01240"
     CDS             complement(7288..8016)
                     /locus_tag="VFR57_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0258029.1"
                     /translation="MPRQARFVVGGVAAHITQRGHNGDMCFIGQSDYLLYLLHLRELS
                     EKHGCAVHAYCLMTNHVHLLLTPDTEQACANLMRDLGQRYVQHFNKTYGRKGTLWQGR
                     YWANIAQSARYVIDCYRYIERNPVVAHMVSAAADYPWSSAAVNTGVREDRLIQPHAEY
                     LALSEDALRRHSVYRDLLSQALDAQLVRDIRESRDTGYPLASDAFKSQLAERLGRKTG
                     PGQPGRPRKRIEMKSGSDPDFFSI"
     gene            complement(8078..8737)
                     /locus_tag="VFR57_01245"
     CDS             complement(8078..8737)
                     /locus_tag="VFR57_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258030.1"
                     /translation="MARSRITPDYRLGVLLVRAGLLPRSRLDVALARQLGGNGKLGMV
                     LVELGDLDEAELDAVLGLQAFLHQARAEDIILFLRARLGGILIGAGAITEEQLLRALL
                     QQEQTGEPLGEILVRQKAISLAVREGALGFQRTLPSPFRERFRLGAMLIEARIVDALT
                     LEAAMRRQRGARVKLGDALLEMGVITKDTLQASLKRQRRLLAALTAGMALAAEAHGIP
                     A"
     gene            complement(8785..9441)
                     /locus_tag="VFR57_01250"
     CDS             complement(8785..9441)
                     /locus_tag="VFR57_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0258031.1"
                     /translation="MAAFVTVLICSNLIGPAKIVQFDWLPFVFGAGVLFFPISYIFGD
                     ILTEVYGYARARRVIWAGFAGLAFASVMAAVVVWLPPAPFWENQKAYETAFGTTWRIA
                     AASMFAYFCGEFANSFVLAKMKILTAGKWLWTRTIGSTIVGEAVDSTLFYPLAFYNSG
                     LIPNDKLPAIMLAQFGLKVGVEVVFTPITYRIVGFLKRVENEDHYDRGTNFSPFSLKT
                     "
     gene            complement(9468..10001)
                     /locus_tag="VFR57_01255"
     CDS             complement(9468..10001)
                     /locus_tag="VFR57_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0258032.1"
                     /translation="MPRTPLAGFAPPLKKRLVELWGTPPNLYKALGNHPELVAAWAEF
                     SKTLRHDTRTPRALRELVILRGAQVMRSEYEWAQHLAMARKAGVSEAKIKNLASWRTS
                     SEFSEQEKAALALAEAVTEGRVSDEVYARAKKHFDDHDYVELALVAAFYAMVGRMIDA
                     MGVELEPEMNNYSPKLA"
     gene            10020..11204
                     /locus_tag="VFR57_01260"
     CDS             10020..11204
                     /locus_tag="VFR57_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0258033.1"
                     /translation="MDIRGFRLTAVPEDFHDDPYRYYAALREQDPVHELEPGSFFLSR
                     YDDVVAVYRHPGASSDKKIEFKPKFGASPLYEHHTTSLVFNDPPLHTRVRGLLMRALN
                     QRAIARMEAGVVDLVDRLLDGFGRETDFIEAFAARIPVEVIGNLLDIPKDERGPLRAW
                     SLAILSGLEPRPTAAMLERGNSAVQEFKQYLARLVAERRRKPGDPDTDVLTRLSQDET
                     IGALELYHNCIFLLNAGHETTTNLIGNGLWLLFRNPGEMARLRIEPALLPSAIEEMLR
                     YEGPIQLNNRRLLEPFDIGGMRLPAGSLLTLGIGAANRDPIQFAAAERFDVGRRPNRH
                     VAFGHGDHACAGMNVARMEARIAFRQLLARFPRIEPAGDPERDRRIRFRGFRKLPVSA
                     RV"
     gene            11214..12302
                     /gene="hppD"
                     /locus_tag="VFR57_01265"
     CDS             11214..12302
                     /gene="hppD"
                     /locus_tag="VFR57_01265"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU0258034.1"
                     /translation="MASHNPIGTDGFEFVEYAAPDPAALGALFERMGFSAVARHRHKN
                     VLLYKQGDVNFIVNAEPRSFAQNFARRHGPSVCAIAFRVRDAAKAYEALLERGAWGVE
                     SRTGPGELNIPAIKGIGDSLIYLVDRYPENDYGNTIYDVDFKPLPGFEDFWRGQAAAP
                     AGAGLAYVDHLTHNVHRGRMSEWAEFYERLFNFREIRYFDIEGKLTGLKSKAMTSPCG
                     KIRIPINESSDDRSQIQEYLEQYHGEGIQHIAMGATDIYATVDALRERAVELLDTPDT
                     YYELIEKRLPGHGEPVDELKKRRILIDGAPGGGLLLQIFTKTLIGPIFFEIIQRKGDE
                     GFGEGNFKALFESMELEQIRRGALKPAA"
     gene            12299..13078
                     /gene="phhA"
                     /locus_tag="VFR57_01270"
     CDS             12299..13078
                     /gene="phhA"
                     /locus_tag="VFR57_01270"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEU0258035.1"
                     /translation="MINTAGLRGDYSKAGPDYGVEQEWGKYTPEEHALYRRLFKRQSN
                     LVPRYACPEWIEAIGRLDAAQKIPRFDEISSKLKARTGWEIVAVPGLIPDDAFFTHLA
                     NRRFPVTVWLRRPDEFDYIVEPDVFHDFFGHVPLLFDRTYADHLYEYGKGGLKAMRLD
                     AVKMLARLYWYTIEFGLMNTPAGVRAYGAGLLSSGGELAYCVEDPRPRRLPFELERIM
                     RTDYQIDRYQDTYFVIDSFDQLMRETAPDFTPIYARIRSCA"
     gene            13075..14073
                     /gene="galE"
                     /locus_tag="VFR57_01275"
     CDS             13075..14073
                     /gene="galE"
                     /locus_tag="VFR57_01275"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0258036.1"
                     /translation="MNVLVTGGLGYIGSHTCVALVAAGHGVRIVDNLSNAKASVLERL
                     RELTGQKIELHAVDIRDRGALDRAMAGKRIDAVLHFAGLKAVGESVAKPLLYYDHNVG
                     GTVALLEAMERFEIRRFVFSSSATVYGEPERLPITEDHPLRPTNPYGKTKLVVEHLLA
                     DYAAANADFRYAALRYFNPVGAHPSGRIGEDPQGEPQNLVPYVARVAVGKLPALRIFG
                     KDYDTRDGTGVRDFIHVMDLAEGHVAALEMKSCMTVNLGTGRGYSVLEVVDAFRRASG
                     KEIATKFVGRRPGDVGSCYADSSRAERLLGWKAKLGLDQMCADAWRWQSKNPAGYP"
     gene            14098..14298
                     /locus_tag="VFR57_01280"
     CDS             14098..14298
                     /locus_tag="VFR57_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258037.1"
                     /translation="MSELEELERRIRKLSPEDLAKFRAWFAEFDHALWDRQLASDAQT
                     GKLDRVVNEALADYKAGKAKKI"
     gene            14295..14549
                     /locus_tag="VFR57_01285"
     CDS             14295..14549
                     /locus_tag="VFR57_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258038.1"
                     /translation="MNHHASPRFWALYEALPSDIRDLAEKNYRLLNRDPHHPSLHFKK
                     IGQFWSVRIGLQYRALGLPVDDGVYWFWIGTHADYDKMVG"
     gene            14628..15662
                     /locus_tag="VFR57_01290"
     CDS             14628..15662
                     /locus_tag="VFR57_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0258039.1"
                     /translation="MIRKALVGAIALAFGAAAQAQEKYEVKVAEFVGAQHFMTKWIMQ
                     WSEKLEKASNGRLVFKHFPNAQMAATPAHYDLARTGQAEISWFLHGGTPGRFPLTEIV
                     NLPYMVGSAEIGTKVLNDAELRAKYLDAEHRGVKVLMLFTHQPGNVHTTKKPIRTQAD
                     MKGMRIRFASPTVRDFIAGLGATPVGVQPTEILEQLQKGGIDGAFIDYGGAGVAFKMG
                     GTVKFTTEMYSYVTSFGLAANPDWYGKLPGDLRKLIDDSLKGVEKEVGEGWDALDSIG
                     KKLLVDAGAQPIRLTKEEDARFRKIGAEVTEAKLKELQGKGMPAVEVHKLMRSLSDKH
                     EKDSKNFWKQ"
     gene            15687..16145
                     /locus_tag="VFR57_01295"
     CDS             15687..16145
                     /locus_tag="VFR57_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0258040.1"
                     /translation="MVPFFARLFGYLAAFFLTATMMVTVADVFLRTFFGYPVRGVLEL
                     VELGLACTIFLALPAVFLRDEHLVVDVIDHLAKKSVVRLLDLAGAIVSLVVLAVMLWQ
                     MVPLARTMHEFGDVTSDLSLAKIWYWVPVLLGLFASALAAVVFIVRWRRR"
     gene            16175..17446
                     /locus_tag="VFR57_01300"
     CDS             16175..17446
                     /locus_tag="VFR57_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0258041.1"
                     /translation="MALLVVLFLRVPIGVALALVGLCGYAAMDGWRKALSMFGSVPFE
                     LASAYSLSVVPLFILMGAVASRAGMSRELFEAANAVFSGFRGALANATVGACAAFGAI
                     CGSSIATAATFSRVAIPEMRRHGYHDAIAAGSVAAAGTLGILIPPSVILAIYSLVAEQ
                     SLAKLFAAALIPGLLLAALYVITVVIVARVRPAWTPRVAGMTMRERLRGARGMWKLAL
                     LFFFAVIGIYLGWFSPTEGAAISAFAAIVIGFATRTLGLRGLLDALLETVHSTAMLFF
                     IIAGAFIFSKFIVLTRLPQELVSWVNAMGFTPLLIIVCVVVLYIVLGTALEEVSTILI
                     TVPVILPLMASLGYDGIWFGVFVTVMATIGLISPPVGLTVFVIQAQNPDIPTRQIYAG
                     TLPFLVADLVLVVLLVAFPALALWLPAKIGL"
     gene            17488..17871
                     /locus_tag="VFR57_01305"
     CDS             17488..17871
                     /locus_tag="VFR57_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEU0258042.1"
                     /translation="MPGRSRDWLKQAAHDLEQARASQRDGRHDWACFAAQQAAENAAK
                     AMHLANGQDAPGRVVATLLAQLPQRAPAALIDKARVLDDVATRYANGHPTAAPFEHYG
                     ILQSQQAIDYAGEILEYARTEVGFS"
     gene            17933..18934
                     /gene="pseB"
                     /locus_tag="VFR57_01310"
     CDS             17933..18934
                     /gene="pseB"
                     /locus_tag="VFR57_01310"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEU0258043.1"
                     /translation="MLDGATILVTGGTGSFGHTFLPMTLARFKPRRLIVYSRDEMKQW
                     EMAKTYGADARVRFFIGDVRDKERLHRALDGVEYVVHAAATKIVPTAEYNPFECVKTN
                     VNGAMNLIDACIDQKVKRAVALSTDKASSPINLYGATKLASDKLFVAGNSYAGAHNTR
                     FSVVRYGNVMGSRGSVIPLFASLAKAGKKLPITDTRMTRFMITLERAVELVWHAFADM
                     EGGEIYVKKIPSMKLTDIARAIAPEAQTEVVGIRPGEKLHEQMIGEEDAHYTYEYDEY
                     YKILPAIHSWSEDANRIKGGRKVAEGFAYTSDGNKDWMDVASLRKWIDANRGKIGTI"
     gene            18936..20090
                     /gene="pseC"
                     /locus_tag="VFR57_01315"
     CDS             18936..20090
                     /gene="pseC"
                     /locus_tag="VFR57_01315"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HEU0258044.1"
                     /translation="MQYIPYGRQDISEADVEAVVRVLRGEYLTQGPAVPAFEEAIQRF
                     CAVKHAVAANSGTSALHLACLALGVGPGDVVWTSPITFVASANCARYCGADVDFVDID
                     PVSCNLDVAALEAKLEHVARMPKAVIPVHLGGLPCDMTEIGRLAQRYGFKVIEDASHA
                     LGSTYRGERVGSCRYSDITVLSFHPVKLITSAEGGMATTNDAALARRMALLRTHGITR
                     EPAEMTHEPDGAWYYQQTELGLNYRMTDVHAALGRSQLERLEAFLKRREELASRYDRL
                     LADTGLRLPARDAHSTSAWHLYIVGWNSKRSGLTRAQAFKRLRDAGIGVNVHYIPVHL
                     QPYYRSLGFRAGQFPNAEAYYADAITLPLYASMSDAQQDRVVDTLRGMLK"
     gene            20087..20794
                     /locus_tag="VFR57_01320"
     CDS             20087..20794
                     /locus_tag="VFR57_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEU0258045.1"
                     /translation="MSTAIVVQARMGSSRLPGKVLKDIAGRPMLSYQMERLRRVKRAE
                     RIVVATTDQPADDAVERFCQKEKIACVRGSEHDVLARYHLAIERFPADVVVRITADCP
                     LIDPAIVDEAIAAYEPDYVSNMLETTYPYGMAVEVFSAQALREAHREAKDPAEREHVT
                     PFIYRHPERYRLRSLTMAPNLSHHRWTVDTPEDFELVSRLLKTLKPHFTLQDVLAVLD
                     KHPDWCAINAHVEQKAL"
     gene            20794..21414
                     /locus_tag="VFR57_01325"
     CDS             20794..21414
                     /locus_tag="VFR57_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid biosynthesis-associated
                     methylase"
                     /protein_id="HEU0258046.1"
                     /translation="MGYKTEQEEFWAGEFGTDYIQRNTGPELLASNLGFFCKALARAA
                     RPASCIEFGANIGMNLRALKLLYPSLEQHAIEINATAAKELATFLPPANVHQASILDF
                     KPTRTWDLVLIKGVLIHMNPESLPAVYEALHKATGRYLLVCEYYNPAPVTVTYRGHTN
                     RLFKRDFCGEIMTRFPDLQLADYGFAYRRDPSFPQDDITWFLMEKH"
     gene            21416..22567
                     /locus_tag="VFR57_01330"
     CDS             21416..22567
                     /locus_tag="VFR57_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEU0258047.1"
                     /translation="MLRYCKRCVMPDTKPDLRLDDDGICNACRSYERRATIDWAARGR
                     ELESILKKYRNPSGKHWDCIIPVSGGKDSTTQTVRLLQLGMNPLCVTATTCDLSAIGR
                     KNIENIKRLGVDYMEFSPNPVVRAKLNRIGLTQVGDISWPEHVGIFTIPVRAAVQFNI
                     PLIIWGENSQNEYGGPAAAAENNTLNRRWLEEFGGLLGLRVSDLVGMEGLEHRHLIPY
                     QYPSDEELNRVAVTGLFLGYYLPWDGLSNALVAQANGFTTFDRAIEGSMVNYENLDNH
                     QTGIHDYFKFLKFGFARATDIACLHVRRGRITREDALDIVRRRDGAFPWTYLGKPLAE
                     ILAPLDMSVDEFVRICDKFTNKKIFKRDANGQLLKDRFGNLTKVNYDNA"
     gene            22564..23175
                     /gene="hisH"
                     /locus_tag="VFR57_01335"
     CDS             22564..23175
                     /gene="hisH"
                     /locus_tag="VFR57_01335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU0258048.1"
                     /translation="MKVAVIDYGLGNLGSVRRAIAELGAEPVLADKPEQLAAADRVIL
                     PGVGSFADGMVLLRERGWVGALRETRKPVLGICLGMQLLASRGTEGGVTEGLDLIPGE
                     VVALETLGCELRIPHVGWNALTVSHPSGIVRGIPDGTDFYFVHSFVFQPGEATDVVAT
                     TSYGVPVTAVVGRGNVMGTQFHPEKSSKAGFRVLRNFLDAGAC"
     gene            23169..>23783
                     /locus_tag="VFR57_01340"
     CDS             23169..>23783
                     /locus_tag="VFR57_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEU0258049.1"
                     /translation="MLKARIIPTLLWKNVGLVKGIGFDSWRRVGPVLPAIKVYNSRDV
                     DELVLVDITASHDKTEPDWESVHDFADECFVPLTVGGGITNVEHIRQLLRAGADKVSI
                     NTAAYANPKLVEDAARRFGSQCVVASIDARRADDGRYICYSHSGTVATGRAPDDWARE
                     LEARGAGEILLTSIERDGTMQGYDLELIASVVHAVNVPVIASGGA"
BASE COUNT         4299 a         7868 c         7499 g         4117 t
ORIGIN      
        1 gtactcgaca tgcgcggtgt tgatcgtgat gccgcgcgcc ttctcttcgg gcgccgcatc
       61 gatctggtcg tagttcttcg cctccccgcc gtacttcttc gccaaaatgt gcgtcatcgc
      121 cgccgtcagc gtggtcttgc cgtgatcgac gtgcccgatc gtgcccacgt tcacgtgggg
      181 tttgttgcgc tcgaatttgc cttttgccat ctaggtcttc cttggtgccc atgacgcgga
      241 ttgaacgcgt gacctctccc ttaccaaggg agtgctctac cactgagcta catgggctga
      301 atattcagta aaccgcgggt cctcaagtag ctggagcggg tgaagggaat cgaaccctcg
      361 tcataagctt ggaaggcttc tgctctacca ttgagctaca cccgcctgag ttgccttcac
      421 cccaaaaaag tcgtcgccgt gagcaggtcc cgagtcgctg gtggaggggg ttggattcga
      481 accaacgtag gcgcaaggcc aacagattta cagtctgccc cctttagccg ctcgggcacc
      541 cctccggcgg aacccgaaat tatgtggatt atccgacgct tggtcaacag gattcaggtt
      601 ccgtatcgaa cccgcgaacg cagcgcttcg agcgcagcga gcgcgctgta gcaggcgagc
      661 caggacgttt tcggattctg cggcgccggc acgttctcga gcaccaccgt gatgcggccg
      721 caagcgcccg acacacgaat cgtgtgggta tttagcgtga gtgcgggatc agcaaccacc
      781 ttgagacgcg tgcgatccat gccaagcccg gcaagcgaca gcaccgccgc gatattcacg
      841 ttctgtggaa agtgcgggac gccctcgcgc gcagggcctt cgaagagcgt cgtcgcgccc
      901 ttgaggcgct cgaggtcgag cccaagcgtc tcgacgtagg cgatgccctt ccaggcctgc
      961 ggcggcttgg cgacctggat cgaaacttca tcgacgcctc ctatgcacgc cgtcttgagg
     1021 gcatccagcc cgccaatacc cccggaaggc acgtagaaga gcgccccagt gcgctgcgcc
     1081 gccgcctcgg cacgcgcgcg caggccatcg tcggcgagcg cgccggcgga aagcacgaca
     1141 acaccgaccc cggcatcgag cagcggtaca agatgctcgc gcaccgcgtc gtgcgacgca
     1201 gcctcgatca cgacctcggg ccgcgcgccg agtaactgtg ttcggtccga gacatacggc
     1261 acgccgaact cgcgcgcgag cgcgacgcca cgggacgccg ggccgcgacc gcacagcgct
     1321 gcaaattcaa ttccgggcgg acggcgctcg agcaccagcc gcgcaatagt gccgccgccg
     1381 agcaacgcga cgcgcatggt ggtctccagt atcattctcc ccgcagcaaa actgcaggga
     1441 ggcatcggtt tgcgcatcgc gattctagaa gacgatcccg accagctcgc gctcttgcgg
     1501 cgctggatct ccgaagacgg ccacgacgtc cacgcctatc tcgccgggcg cgaggccatg
     1561 aagcaggccg ggcgcgagag cttcgacctg ttcatgcttg actggcaggt gcccgacgtc
     1621 tcgggcgccg aggtcctgat gtggatccgg gccaacgtgt cgaagtccgt ccccgttctc
     1681 ttcgtcaccg tgcgcgactc cgagcaggac atcgtgttcg cgctcgagca cggcgcggac
     1741 gattacatgg tgaagccggt gcgccggcag gagctcctcg cgcgcgtgca cgccctgctg
     1801 cgccgcgcct atccgaccga ggagaaaaag cagctctcct tcccgccgtt cgagatcgac
     1861 acccagcgcg ccgaagtgcg ccgcgacggc gtagtcatcg acctcacgcc aaaggaattc
     1921 gagctcaccg tcacgctttt ccgcaacatc gggcgcctgc tttcgcgcgg acatctgcag
     1981 gaaagcgtat gggggcgcac cggagacctc gccacgcgca ccgtcgatac gcacgtgagc
     2041 caggtccgca agaaactcga tctgcgcccg gaatcgggtt accgggtggt accgatctac
     2101 aactacggct accggctcga gaagatctcg ccggcggcgt gaccgtccgt cgatagggcg
     2161 cgaaatcggg ctctgacccc gatttcttaa gcaacccgcc gtagaaaaaa taagggggca
     2221 ggcttttggc ctgccccccg atcagcccga cggtattgga ggaggaggag gaaccaaccg
     2281 ggctgctgca acgcaacaac tgtacggctt tgctggcgcg cttagaatac gctggaatcg
     2341 taaaacactt ttgctgattc cgcaaagatg cctaacgcgc tgcaggagct aacgattttc
     2401 gccaaggtgg tggggaccgg cagcctttcg gcggccgcac gcgacttggg catgtccccg
     2461 gccgtagtga gccgacggct cgcttccctc gaagcgcgtc tcggcgtgcg gctcgtaaac
     2521 cgcaccaccc ggagcctgca cctcaccgac gaaggcgccg cctactacga gacctgtacc
     2581 cgggtgctcg ccgacatcga ggaggccgac gcggccgttt ccgccggtcg ggccgagccc
     2641 cggggcgtgc tgcgcgtcgc gctgcccgca tcgttcggca accagcacgt cgcgccgctc
     2701 gtcccgaagt tcgcccagct ctatccgaac gtgcagatcg cgctcagcct ctccgatcgc
     2761 accgtcaacc tcgtcgagga aggcttcgat ctggcgatcc gcatcgccga ccttgccgac
     2821 tcggcgctcg ccgcgcgccg gctcgcaccc aatcgccgcg tcgtatgcgc aagtccggca
     2881 taccttcgcc gcaacggcac gccggcgacg cccgaggatc ttgcgaagca caattgcctc
     2941 acgaccaccg acttcgcgat gaactgggac taccgcgcgc ccgacggcaa agccggctcc
     3001 gtacgcgtcg ccggccgcta tgcctgcgat aacggggagg tgctgcgcga atgggcgctc
     3061 gcgggccttg ggatcgcgct gaagtccacc tgggacgtac gccgtcacct ggaagacgga
     3121 tcgctcgttt cgctacttcc gggctatacc ttcgcgagcg acgtggccat ctacgcggtc
     3181 tatcctcatc gcaggcacct gcccgcaaag acgcgtgcgt tcattgaatt cctggccgac
     3241 tcattcggcc ctgagccata ctgggaccga gagaaatcgg cgtcggacga gcggaaatcg
     3301 ggctctgacc ccgatttccc gccgaaaccg agaatcaagg ggcgctcgcc tgtctaactg
     3361 aacgatgttc agcctcgaag acaagtacac cctggcaaac ggcacggtct ttctgaccgg
     3421 catgcaggcg ctggcgcgcc tcatgatgct gcaacgcgaa cgcgacctgg gcgcaggcct
     3481 gaacaccgcg gggttcatct cgggctaccg cggctcgccg ctcggcggct tcgatcagac
     3541 gctcgcgcgc gcaagagcgc acctcgagaa aagccacgtg cgtttccagc cgggcctcaa
     3601 cgaagatctc gccgccaccg cgatctgggg cacgcagcag ttgaacctct tccccggcgc
     3661 gaattacgac ggcgtgttct cgatgtggta cggcaagggt ccgggcgtcg atcgctgcgg
     3721 cgacgtcttc aagcacgcga acgccgcggg cacttcgaag cacggcggcg tgctcgtact
     3781 ggcgggcgat gaccacggcg cgaaatcgtc gacgctcccg caccagtcgg agcacatctt
     3841 caaggcgtgc ctcatcccgg ttctcaaccc ggcgaacgtc caggattacc tcgacctcgg
     3901 cctgcacggc ttcgcgatga gccgctattc gggctgctgg gttgccttca agtgcgtcac
     3961 cgacatcgtc gagtccggcg catcggtcgt cgtcgacgcc aaccgggtcg caatccgcac
     4021 gcctggcgac tttgtgctac cgcccgacgg cctcaatatc cgctggcccg acgggatcct
     4081 cgagcaggaa gcgcgcatcc tcgactggaa ggtctacgcc gcgcttgcct acgtgcgcgc
     4141 gaacggtctg gaccgcatcg tctacgactc gccgaaagcg cgcctcggca tcgtcacgac
     4201 cggcaagtcg tacggcgatg tgatgcaggc gctcaacgat cttggcatca cgaaagacgt
     4261 ggcgcgcgac atcggccttc gggtctataa ggtcgccatg tcgtggccgc tggaaccgca
     4321 aggcgcgcgc cgcttcgccg acggcctcga agagatcctc gtcgtcgagg agaaacggca
     4381 gctgatcgag taccagatca aggaagagct ctacaactgg aaggaaggcg tgcgcgcacc
     4441 gcgcgtcgtc ggcaagttcg acgactcggg cgaatggagc cgcagcggcg gccggcccgc
     4501 gggcacctgg ctcctgcctg cgcactacga gcattcgccc gcgatggtcg ccaaggcgat
     4561 cgcgcagcgc ctcgagaagc tcgggatggc gaatcgactc gggtcccagt tccgcgagcg
     4621 cctcgcgttc ctcgacttca aggaaaagat cctgaagcgg ccgcgcgtca cggcggtgcg
     4681 ccaaccctat ttctgctcgg gctgcccgca taacacgtcc acgcgcgttc ccgaaggcag
     4741 ccgcgccacc gccggcatcg gctgccattt catggccgtg tggatgaacc gctcgaccgc
     4801 gaccttcacg catatgggtg ccgaaggcgc gccgtggatt ggccaggcgc cgttcaccaa
     4861 agagaagcat atcttcgcga acctcggcga cggcacgtac ttccattccg ggatcctcgc
     4921 gatccgcgcg gccgtcgcgg cgaaggtcag aatgacctac aagatcctct acaacgacgc
     4981 cgtcgccatg accggcggcc agcgccacga cggtccgctc gatccgccca cgatctcgcg
     5041 ccagctcgcg gccgaaggcg tggcgccgat cgttgtcgtc accgatgagc cgacgaagta
     5101 cccggccgca accgcgtggg ccaagggcgt cacgatccgt caccgcgacg acctggatgc
     5161 agttcagcgc gaactccggg aaaccaaggg ggtgtcggcg ctcatctacg accagacctg
     5221 cgcttccgag aagcgccggc gcaggaagcg cgacgaatat cccgatcccg cgaagcgcgt
     5281 cttgatcaac gagatggtgt gcgagggctg cggcgactgc tcggtcaagt cgaactgcct
     5341 ctcggtcgag ccgctcgaaa ccgagttcgg ccggaagcgt cagatcaacc agtcgtcatg
     5401 caacaaggac tattcgtgcg tgaaggggtt ctgcccgagc ttcgtcacca tcgagggcgg
     5461 gcgcctgcgc aaagggaaag cggcgccgaa ggaaaccgaa ggcgaagtcc cgtctccgca
     5521 gattccgagt ctggctgcgc cgtacggcat ccttgtcacc ggcatcggcg gcaccggcgt
     5581 catcaccatc ggacagatca tcggcgttgc ggcgcacgtc gaaggcaagg gcgtgacggt
     5641 gctcgacatg tccggcctcg cgcagaagta cggcgccgtg atgtcccatg tccaggtcgc
     5701 ggcatcgccg gatcagatgc acgccacgcg catcgacacg ggcggcgcga atctggtgct
     5761 cggctgcgac ctggtcgtga ccgcgagcac cgagtcgctc gccaagatgg ccccgaagcg
     5821 cacgcgcgcg ctcgtgaacg cctcggtcac acccaccgcc gagttcgtac gcaatccaaa
     5881 ctggcagctc cccggcaccg atctcacgaa ggatgtggcg gagtcgtgcg aaaaggccga
     5941 gtttgtcgaa gccacggcca tcgccaccgg cctcatgggc gacgcgatcg cgaccaacat
     6001 gttcatgctg ggctacgcgt accagaaggg ctggatcccg ctcgccgccg agtcgctcga
     6061 gcgcgcgatc gagctgaacg gcgtggcggt cgaattcaac aggaagagct tcgtctgggg
     6121 tcgtcgcgcg gcgctcgatc tcgagaaagt gcgccgcatc gcgatgcccg cggaagtgat
     6181 cccgatcgag cagcacttct ctcgcaatct ggacgagctt atcgagcgcc gcgtcaggtt
     6241 gctcaccgag tatcagaacc gcgcctatgc ggaccgctac cgcgcgctgg ttgaaaaggt
     6301 gcgcgcaaaa gacgagaagc tggccgaaat cgtggcgcgc aactacgcga agctgctcgc
     6361 gtacaaggat gaatacgaag tcgcgcgcct gcacgctgac gcgaacttcc gcaagaagat
     6421 cgagggcatg ttcgagggcg actatcgcgt cgtctaccac ctcgctccgc cgcttctcac
     6481 gcgaaaagag gagaaggtgc gcttcgggcc ttggatgctg cccgtcttca aggtgctgag
     6541 gaacttcaag ttcctgcgcg gcagcgcgct cgatccgttc ggctatacgc aagagcggcg
     6601 caccgagcgc gctttgatca gcgagtacga ggacacggtc gcgcggctgc tcgcgcgtct
     6661 gaactcacag aacgaagccc tcgcgctcga gatcgcggcc atcccggacg agatccgcgg
     6721 cttcggccac atcaaggcgc gcaacctcgc tccggcgcgc aagaagtggc aggaactcct
     6781 cgcccgctac gaggccggtc agcccgcgca gcgtgcggcg gcttgaatta gatacgtcgc
     6841 ccccgcgaag ccgggggccc cgttggcttt ttcttctctt gatgttgatg gcgagcgctg
     6901 cagcagcgca gcagcgcctc ctcgacgctg attccccgga cagcgcacgc ccgaccgcta
     6961 tgaaagtcct gcgcctcctc gccgacggcg acatcgagac cgcggccaaa acttccaacg
     7021 cccccgagcg ccggctcgaa gtgttgcgcg actaccgcaa aagggttggc gatgaggaat
     7081 tcaagcgcgc tttcgcccgt tactttgaaa ccccggttgt cgccgaagtc gcgctcggcc
     7141 cccggcgcct cctcgtttgg aacctcggcg aggacctcgc cgcccagtac tacatcgaac
     7201 gtgagggccg cttcgtcatg gacgacgtgc ccagtcccgc ccgtgcggag ctcgcgcgca
     7261 tcctgcgcga gttcaggtcg ggtcacctca aatactgaaa aaatcggggt cagagcccga
     7321 tttcatttcg atgcgctttc gcggccgccc cggctggccc ggacccgtct tccgaccaag
     7381 gcgctcggca agttgcgact tgaaggcatc gctcgccaat ggatatcccg tgtcccggct
     7441 ttcgcgaatg tcgcgcacca gttgggcatc tagcgcttgg cttagtaagt ctcggtacac
     7501 ggaatgtcgt cgaagcgcgt cctcactaag cgcgaggtat tccgcatgcg gctggatcag
     7561 acgatcttcg cgcacgcctg tattcacggc cgcgctggac caagggtagt ccgcggcggc
     7621 gctcaccata tgagcgacta ccgggtttct ctcgatatag cgataacaat cgatcacata
     7681 gcgcgcggac tgcgcgatgt tcgcccagta gcggccttgc catagcgtgc ccttgcggcc
     7741 gtaggtctta ttgaagtgct ggacgtagcg ctgaccaagg tcccgcatca gattcgcgca
     7801 ggcctgttcg gtgtccggcg tgagaagcaa gtggacatgg ttcgtcatca ggcaatacgc
     7861 gtgcaccgca catccatgct tttcactcag ctcgcgtaag tgaagtaggt agagcaggta
     7921 gtcgctctgc cctatgaagc acatatcgcc gttatggccg cgttgcgtta tgtgcgcggc
     7981 gacgccgccg actacaaaac gcgcttgcct gggcatgcca gacaaacggc cggcaggcgg
     8041 tgggcgttga ctgtaatcgg gctctgaccc cgatttttta cgccgggatg ccgtgggctt
     8101 cggccgcgag cgccatgccg gcggtgagcg cagcgagcag gcgacgctgg cgcttgaggg
     8161 aggcctgcag cgtgtccttg gtgattacgc ccatctcgag gagcgcatct ccgagcttga
     8221 cgcgcgcgcc gcgctggcgg cgcatggcgg cttcgagggt cagggcatcg acgatgcgcg
     8281 cctcgatgag catcgcgccg aggcgaaagc gttctcgaaa cgggctcggc agcgtgcgct
     8341 gaaaaccgag cgcgccttca cgtacggcaa ggctgatcgc cttttgccgc acgagaatct
     8401 cgccgagcgg ctcacccgtc tgctcctgct ggagaagcgc gcgcaggagc tgctcctcgg
     8461 taatcgcgcc cgcgccgata agaatgccgc cgaggcgtgc gcgcagaaag aggatgatgt
     8521 cctccgccct ggcctgatgc aggaaggcct gtagtccgag aaccgcgtcg agctcggctt
     8581 catccaggtc gcccagctcg acgagcacca tgccgagctt gccattgccg ccgagctggc
     8641 gcgcgagcgc gacgtccagt cgcgagcgcg gcagcagccc ggcgcgtacc aggagcacgc
     8701 cgagccggta gtcgggagtg attcttgagc gtgccaagct tggcgatgat ggttccgccg
     8761 cttccgcttg gtcgaggaac tattctaggt cttcaggctg aacggactaa agttggtgcc
     8821 gcggtcgtaa tgatcctcgt tctcgacgcg cttaagaaag ccgacaatcc ggtaagtaat
     8881 gggggtgaac acgacttcga ccccgacttt gagcccgaat tgcgcgagca tgatcgccgg
     8941 cagcttgtcg ttcggaatga ggccagagtt gtagaaggcg agcgggtaga aaagcgtgga
     9001 atcgaccgcc tcgccgacga tggtcgagcc gatggtgcgc gtccagagcc acttcccggc
     9061 ggtgaggatc ttcatcttcg cgagcacgaa cgagttcgcg aattcgccgc agaaataggc
     9121 gaacatcgaa gccgccgcga tgcgccacgt ggtgccgaac gcggtctcgt acgccttctg
     9181 gttctcccag aacggcgcgg gcggcagcca caccaccacg gcggccatga ctgaggcgaa
     9241 agcgagcccg gcgaagcccg cccagatcac gcgccgcgcg cgcgcgtagc cgtacacctc
     9301 ggtgaggatg tcgccgaaaa tataggagat cgggaagaac agcacgcccg cgccgaagac
     9361 gaacggcagc caatcgaact gcacgatctt cgccgggccg atcaggttcg agcagatgag
     9421 caccgtgacg aacgccgcca tcacgaactc gtaatagcga tagttcttca agcgagcttc
     9481 ggcgaatagt tgttcatctc gggctcgagc tcgacaccca tcgcgtcgat catgcgcccc
     9541 accatggcgt agaacgcggc caccagcgcg agctcgacgt agtcgtggtc gtcgaagtgt
     9601 ttcttcgccc gcgcgtacac ctcgtcgctg acgcgtccct cggtcaccgc ctcggcaagc
     9661 gcgagcgccg ctttctcctg ctccgaaaat tcagacgaag tgcgccacga cgccaggttc
     9721 ttgatcttcg cctccgagac gccggccttg cgcgccatcg cgagatgctg cgcccactcg
     9781 tactccgagc gcatgacctg cgcgccgcgc aggatgacga gctcgcgcag cgcgcgcgga
     9841 gtgcgcgtgt cgtggcgcag cgttttcgaa aactcggccc acgccgcgac gagctccggg
     9901 tgattgccga gcgccttgta gagattgggc ggcgtgcccc acagctcgac gagccgtttc
     9961 ttgagcggtg gcgcgaaacc ggcgagaggc gtgcgcggca tggggcggga taataactga
    10021 tggatatacg cggcttccgg ctcaccgcgg tgccggagga cttccacgac gacccctatc
    10081 gctactacgc cgcgctgcgc gagcaggacc cggtgcacga actggagccg ggcagctttt
    10141 tcctctcgcg ctacgacgat gtggtcgcgg tgtatcgcca tcccggcgcg agctccgaca
    10201 agaagatcga gttcaagccc aaattcggcg cctctccgct ttacgagcac cacaccacga
    10261 gcctggtgtt caatgatccg ccgctgcaca cgcgcgtgcg cggacttctc atgcgcgcgc
    10321 tcaaccagcg cgcgatcgcg cgcatggaag cgggcgtcgt cgatctcgtc gatcgcctgc
    10381 tcgacggctt cggcagggaa accgatttca tcgaagcatt cgcggcgcgc attcccgtcg
    10441 aggtgatcgg caacctgctg gacatcccca aagacgagcg cgggccgtta cgcgcctggt
    10501 cgcttgcgat cctttccggg ctggagccgc ggccgacggc cgcgatgctc gagcgcggca
    10561 atagcgcagt gcaggagttc aagcaatacc tggcgcgctt ggtggccgag cgccgacgca
    10621 agccgggcga cccggatacc gacgtgctca cgcgcttgag ccaggacgag acgatcggcg
    10681 cgctcgagct ttaccacaac tgcatattcc ttctgaacgc gggccacgag acgaccacca
    10741 atctcatcgg caacggcctc tggctgctct ttcggaatcc gggcgagatg gctcgcctca
    10801 gaatcgagcc ggcgcttttg ccttcagcca tcgaagagat gctgcgctac gaaggaccga
    10861 tccagctcaa caaccggcgc ctccttgaac ctttcgacat cggcggcatg cgtctgcccg
    10921 ccggttcgct tctcaccttg ggcatcggcg ccgcgaatcg cgacccaatc cagtttgccg
    10981 ccgccgagcg tttcgatgtc ggccgccgtc ccaatcgcca cgtagctttc ggccatggcg
    11041 atcacgcctg cgcgggcatg aacgtggcac gcatggaagc gcgtatcgcc ttccggcagc
    11101 tgcttgcgcg ctttccgcgc atcgagccag ccggcgaccc cgagcgcgac cggcgcatcc
    11161 gcttccgggg ctttcgaaaa ctccccgtta gcgcgagggt ctaagattgc gcgatggctt
    11221 cgcataatcc gatcggcacg gacggtttcg aattcgtcga gtacgcggcg cccgacccgg
    11281 cggcgctcgg cgctctcttc gagcgcatgg gcttcagcgc ggtggcgcgg caccgtcaca
    11341 agaacgttct tctctacaag cagggcgacg tcaatttcat cgtgaacgcc gagccgcgct
    11401 cgttcgcgca gaacttcgcg cggcggcatg ggccgagcgt gtgcgccatc gcctttcgcg
    11461 tgcgcgatgc ggcgaaggcg tacgaggcgc tgctcgaacg tggcgcctgg ggcgtcgaga
    11521 gccgaaccgg gccgggtgag ctcaacattc cggcgatcaa gggtatcggc gattcgctga
    11581 tctatctcgt cgaccgctat ccggagaacg attacggcaa cacgatctac gacgtcgatt
    11641 tcaagccgct gcccgggttc gaagatttct ggcggggtca agcggccgcg ccggcgggcg
    11701 cgggtctcgc ctacgtcgat catctcacgc acaacgtcca tcgcggacgc atgagcgagt
    11761 gggcggagtt ctacgagaga cttttcaact tccgcgaaat tcgctacttc gacatcgaag
    11821 gcaagctcac gggactcaag tccaaagcga tgacgagccc gtgcggcaag atccgcattc
    11881 cgatcaacga gtcctccgat gatcgctcgc agatccagga gtatctcgag cagtaccacg
    11941 gcgaaggcat tcagcacatc gcgatggggg ccacggacat ctacgcaact gtggacgcgc
    12001 tgcgcgagcg cgcggtcgag ttgctggaca cgccggacac gtactacgag ctcatcgaga
    12061 agcgtctgcc cggccacggc gagccggtgg acgagctcaa gaagcgcagg atcctgatcg
    12121 acggcgcgcc cggcggtggc ctgctgctcc agatcttcac gaagacgctg atcgggccga
    12181 tcttcttcga gatcattcag cgaaagggcg acgaaggttt cggcgaaggc aatttcaagg
    12241 cgctgttcga gtcgatggag ctcgagcaga tccgccgcgg agcgctcaag cccgccgcat
    12301 gatcaacaca gccggcttgc ggggcgacta ctccaaagcc ggaccggatt acggggtcga
    12361 acaggaatgg gggaagtaca cgccggagga gcacgcgctc taccggcgtc tcttcaaacg
    12421 ccagtcgaac ctcgtgccgc gctatgcctg cccggaatgg attgaggcga tcgggcggct
    12481 ggatgcggcg cagaagattc cgcggttcga cgaaatctcg agcaagctaa aggccaggac
    12541 cggctgggag atcgtcgccg tgccgggcct tattcccgac gatgcgttct tcactcacct
    12601 cgccaaccgc cgctttccgg tcacggtgtg gctgcgtcgg cctgacgagt tcgactacat
    12661 cgtcgagcct gacgtattcc acgacttctt cgggcacgtg ccgcttcttt tcgaccgcac
    12721 ctacgccgac cacctctatg aatacggcaa gggcgggctg aaggcgatgc gcctggacgc
    12781 tgtgaagatg ctggcccggc tctactggta cacgatcgag ttcggcctca tgaatacccc
    12841 ggccggcgtg cgcgcttacg gcgcggggct cctctcctcc ggcggcgagc tcgcgtactg
    12901 cgtggaggac cccaggccgc ggcgcctgcc gttcgagctc gagcgcatca tgcgcaccga
    12961 ttatcagatc gaccgctacc aggacacata tttcgtcatc gactccttcg accaactgat
    13021 gcgcgaaacg gcgcccgact tcacaccgat ttacgcgcga atcaggtctt gcgcttgaac
    13081 gttctcgtca ccggcggact cggctatatc ggctcgcaca catgcgtcgc gctcgtggcg
    13141 gcggggcatg gcgtgcgcat cgtcgataac ctctcgaacg cgaaggcgtc ggtcctcgag
    13201 cgactgcgcg agctcaccgg gcagaaaatc gagctgcacg cggtggatat ccgcgatcgc
    13261 ggcgcgcttg atcgcgcgat ggccgggaag cgaatcgacg cggtgctgca tttcgctggc
    13321 ctcaaggcgg tcggcgagtc ggtcgcgaag ccgcttctct attacgacca caacgtcggc
    13381 gggacggttg cgctcctcga ggcgatggaa agattcgaaa tccgacgctt tgtattcagc
    13441 tcgagcgcga cggtgtatgg cgagcccgaa cgcctgccga tcaccgagga ccatcccctg
    13501 cggcctacga acccttacgg taagacaaag ctcgtcgtcg agcacttgct cgccgattac
    13561 gccgcagcga atgcggattt ccgctacgcc gcccttcgct atttcaaccc ggtcggcgcg
    13621 catccgtccg ggcgcatcgg cgaggacccg caaggcgagc cgcagaacct cgttccgtat
    13681 gtcgcgcgcg tcgccgtggg caaacttccg gcacttcgca tcttcggcaa ggactacgac
    13741 acgcgcgacg gcaccggcgt gcgcgatttc atccacgtca tggacctcgc cgaaggccac
    13801 gtcgcggcgc tcgaaatgaa gtcttgcatg acggtcaatc tggggacggg tcgcggctat
    13861 tctgtgctgg aggtcgtgga cgcgtttcgc cgcgcctccg gcaaggagat tgcgaccaaa
    13921 tttgtcggcc gccggcccgg tgacgtgggc tcctgctacg ccgactcgag tcgcgccgag
    13981 cggctgctcg gctggaaagc gaagctgggg cttgaccaga tgtgcgccga tgcgtggcgc
    14041 tggcagtcga agaatcccgc cggttatccc tgagcgcggg ggcgttagaa tcactctatg
    14101 agtgagctcg aggaactcga aaggcgaatc cgaaagctgt cgccagaaga cctggcgaag
    14161 ttccgtgcgt ggtttgctga attcgatcac gcgttgtggg atcggcaact tgcgtctgac
    14221 gcgcagaccg gcaagctcga ccgggtcgtg aatgaagccc tcgcggacta caaggcaggc
    14281 aaggccaaga agatttgaat caccacgcct cgccgcggtt ctgggcactc tacgaggcgt
    14341 taccgtccga cattcgtgat cttgcagaga agaactaccg gttactgaac cgggatccgc
    14401 atcatccgtc tctgcacttc aagaagatcg ggcagttctg gtccgttcgg attggtctgc
    14461 agtatcgcgc gctcggtctg ccagtcgacg atggggtcta ctggttctgg ataggtacac
    14521 atgccgacta cgacaagatg gttggctaac ggcgagtcga ccaaccggct aacctataat
    14581 tcccgagaaa tcgggctctg accccgattt tccgtcgagg aggaagaatg attcgcaagg
    14641 cactggttgg cgcgatcgcg ctcgcgttcg gcgccgccgc gcaggcgcag gaaaaatacg
    14701 aagtgaaggt cgcggagttc gtcggcgcgc agcacttcat gacgaagtgg atcatgcagt
    14761 ggagcgagaa gctcgagaag gcgtcgaacg gccggctggt attcaagcac tttcccaacg
    14821 cgcagatggc ggcgacgccg gcgcattacg acctcgcccg caccggacag gcggagatct
    14881 cctggttcct gcacggcggc acgccgggcc gttttccgct caccgagatc gtgaatctgc
    14941 cctacatggt cggcagcgcg gagatcggca cgaaagtgct caatgacgcg gagctgcgcg
    15001 cgaaatacct cgacgccgag cacaggggcg tgaaggtgct gatgctcttc acgcaccagc
    15061 cgggcaacgt gcatacgacc aagaaaccga tccgtacgca ggccgacatg aaaggcatgc
    15121 gcattcgctt cgcctcgccc acggtgcgcg acttcattgc cgggctcggc gcgacgccgg
    15181 tcggcgtgca acccaccgag attctcgagc agctgcaaaa gggcggcatc gacggcgcgt
    15241 tcatcgacta cggcggcgcc ggcgtcgcgt tcaagatggg cggcacggtg aagttcacga
    15301 ccgagatgta cagctacgtc acctccttcg gcctcgccgc caacccggac tggtacggca
    15361 agctgcccgg cgatcttcgc aagctgatcg acgattcgct gaagggcgtg gaaaaggaag
    15421 tgggcgaagg ctgggatgcg ctcgactcga tcggcaagaa gctcctcgtc gacgctggcg
    15481 cgcagccgat ccgtctcacc aaggaagagg atgcacgctt ccgcaagatc ggcgccgaag
    15541 ttaccgaagc gaagctgaag gagctccagg gcaagggcat gcccgcggtc gaagtgcaca
    15601 agctcatgcg ctcgctgtcg gataagcacg agaaggactc gaagaacttc tggaagcagt
    15661 gaaaatcggg ctctgacccc gatttcatgg tgccgttctt tgcgaggctg ttcggttacc
    15721 tagcggcgtt cttcctcacg gcgaccatga tggtcacggt cgccgacgtc ttcctgcgca
    15781 cgttcttcgg ctaccccgtg cgcggggtgc tcgagctggt cgagctcggg ctcgcctgca
    15841 ccatcttcct cgctctgccc gcggtgttcc tgcgcgacga gcacctcgtg gtcgacgtca
    15901 tcgaccacct ggcgaagaag tcggtggtgc gtcttctcga cctcgccggc gccatcgttt
    15961 cgctggtagt gctcgcggtg atgctctggc agatggtgcc gctcgcgcgc accatgcacg
    16021 agttcggcga cgtcacctcg gacctttcgt tggcgaagat ctggtactgg gtaccggtac
    16081 tcctcggtct cttcgcttcc gcccttgccg ccgtggtctt catcgtgcgc tggagacgcc
    16141 gataagcgca cttgccatcg gcgcctgggg cgtcgtggcg ctcctcgtgg tgctgtttct
    16201 gcgggtaccg atcggcgttg cgcttgcgct cgtgggcctg tgcgggtacg cggcgatgga
    16261 cggctggcgc aaagcgctct ccatgttcgg ctcggtgcct ttcgagctcg cgagcgccta
    16321 ctcgctctcg gtggtgccgc tcttcatcct gatgggtgcg gtcgcctcgc gcgccggcat
    16381 gtcgcgcgag ctcttcgaag cggcgaacgc tgtgttctcg gggttccgag gggcgcttgc
    16441 caacgccacg gttggcgcct gcgctgcgtt cggcgccatc tgcggctctt ctatcgccac
    16501 tgcggcgacg ttctcgcgtg tcgcgatccc cgagatgcgc cgccatggat accatgacgc
    16561 gatcgccgcc ggctcggtgg ccgccgccgg cacgctcggc atcctgattc cgccctcggt
    16621 gatcctcgcg atctactcgc tggtcgcgga acaatcgctc gccaagctgt tcgccgcggc
    16681 gctgatcccg ggactcctgc ttgcggcgct ctatgtgatc acggtggtga tcgtcgcgcg
    16741 cgtgcgcccg gcgtggacgc cgcgcgtcgc cggcatgacc atgagggagc gcttgcgcgg
    16801 cgcgcgcggc atgtggaagc tggcgctgct ctttttcttc gcggtgatcg gcatctatct
    16861 cggctggttc tcgcccaccg agggcgcggc gatcagcgcc ttcgccgcga tcgtgatcgg
    16921 cttcgcgacg cgcacgctcg gcctgcgcgg cctgctcgac gccctgctcg agaccgtgca
    16981 ctccaccgcg atgcttttct tcatcatcgc gggcgccttc attttctcca agttcatcgt
    17041 gctcacgcgt cttccgcagg agctcgtcag ctgggtgaat gcgatgggct tcaccccgct
    17101 cctcatcatc gtgtgcgtgg tcgtgctcta catcgtgctc ggcaccgcgc tcgaggaggt
    17161 gagcaccatc ctcatcaccg tgcccgtgat cctgccgctc atggcgagcc tgggctacga
    17221 cggcatctgg ttcggcgtct tcgtgaccgt gatggcgacc atcggcctca tcagcccgcc
    17281 cgtcggcctg accgtattcg tgatccaggc ccagaacccc gacatcccga cgcggcagat
    17341 ctacgccggc accctgccct tcctcgtcgc ggacctggtg ctggtcgtgc tcctggtcgc
    17401 cttcccggcg ctcgcgctct ggctccccgc gaaaatcggc ctctaagaaa tcggggtcag
    17461 agcccgattc tttactaaca tcgccccatg ccgggccgtt cacgcgactg gttgaagcag
    17521 gcggcgcacg acctcgagca ggcgcgcgcg tcgcaacgcg acggccggca cgattgggcg
    17581 tgctttgctg cccagcaggc cgccgaaaac gccgccaagg cgatgcatct tgccaacggg
    17641 caggacgcgc cggggcgcgt ggtggcgacg ctccttgccc agcttccgca gcgcgcgccg
    17701 gccgcgctga tcgacaaggc gcgagtgctg gacgatgtgg cgacgcgcta cgcgaacggg
    17761 caccctaccg cagcgccctt cgagcactac ggcattctcc aaagccagca ggcgatcgac
    17821 tatgccggcg agatcctcga atacgcccgc accgaagtag gcttttccta gcgttttccg
    17881 aggtcaagcg ccgcttcctc agttagaatc tgaagattcg gcgggggact aaatgctgga
    17941 cggcgcaacc atactggtga ccggagggac ggggtccttc ggccacacgt tcctgccgat
    18001 gaccctcgcc cgcttcaagc cgcgccggct gatcgtctat tcgcgcgacg agatgaagca
    18061 gtgggagatg gcgaagacct acggcgccga tgcgcgcgtg cgcttcttca tcggcgatgt
    18121 acgcgacaag gagcgcctgc accgcgcgct cgatggcgtc gagtacgtgg tgcatgccgc
    18181 ggctacgaag atcgtgccga cggccgaata caacccgttc gagtgcgtga aaacgaacgt
    18241 caacggcgcg atgaacctga tcgacgcgtg catcgatcag aaagtgaaac gcgcggtggc
    18301 gctttccacc gacaaggcga gcagcccgat caatctctac ggcgctacca agctcgcttc
    18361 ggacaagctc ttcgtcgccg gcaattccta cgccggcgcg cacaacaccc gcttctccgt
    18421 ggtgcgttac ggcaacgtca tgggctcgcg cggctcggtg atcccgcttt tcgcctcact
    18481 cgccaaggcg ggaaagaagc tgccgatcac cgacacgcgc atgacccgct tcatgatcac
    18541 gctcgagcgc gcggtcgagc tcgtctggca cgccttcgcc gacatggaag gcggcgagat
    18601 ttacgtgaag aagatccctt cgatgaagct caccgacatc gcgcgcgcca tcgcgccgga
    18661 ggcccagacc gaagtggtcg gcatccgccc gggcgagaag ctccacgagc agatgatcgg
    18721 cgaagaggac gcgcactaca cctacgaata cgacgagtac tacaagatcc tgccggcgat
    18781 ccatagctgg agcgaggacg cgaatcgcat caagggcggc cgcaaggtgg ccgaaggttt
    18841 cgcctatacg tcggacggca acaaggactg gatggacgtc gcgtcgctgc gcaagtggat
    18901 cgacgccaac cgcggcaaga taggaacgat ctagtttgca gtacatcccg tacggccggc
    18961 aggacatcag cgaggcggat gtcgaggcgg tggtgcgggt gctgcgcggc gagtacctca
    19021 cgcaggggcc ggcggtaccg gcgttcgaag aggcgatcca gcgcttctgc gcagtgaagc
    19081 acgccgtcgc cgcgaatagc ggcacttccg cgcttcacct ggcttgcctt gcgctcggcg
    19141 ttggcccggg cgatgtcgtc tggacttcac ccatcacttt cgtcgcgagc gcgaactgcg
    19201 cgcgctattg cggcgccgac gtcgatttcg tcgacatcga cccggtgtcc tgcaacctcg
    19261 acgtggccgc gctcgaggcg aagctcgaac atgtggcacg catgccgaag gcagtgatac
    19321 ccgtgcacct tggcggcctg ccgtgcgaca tgacggaaat cggtcggctc gcgcagcgct
    19381 atggcttcaa ggtgatcgag gatgcttcgc atgcgctcgg cagtacctac cgcggcgagc
    19441 gcgtcggcag ctgccgctac agcgacatca cggtgctcag cttccatccc gtcaagctca
    19501 tcacgagcgc ggaaggcggc atggcgacca ccaacgacgc ggcgctcgct cggcgcatgg
    19561 cgctgcttcg cacgcatggc atcacccgcg agccagcgga gatgacgcac gagccggacg
    19621 gcgcctggta ctaccagcag accgagctcg gcctcaacta ccgcatgacc gacgtccacg
    19681 ccgcgctggg ccgcagccag ctcgagcggc tcgaagcctt cctgaaacgc cgtgaggagc
    19741 tcgctagccg ttacgaccgc ctgctcgccg acacgggcct gcgcctgccc gctcgcgacg
    19801 cgcacagcac gtcggcctgg cacctttaca tcgtcggctg gaacagcaaa cgctcgggac
    19861 ttacgcgcgc gcaggccttc aagcgtctgc gcgacgcggg gatcggcgta aacgtgcact
    19921 acattccggt gcacctgcag ccgtattacc gcagcctcgg ctttcgcgcc ggccagtttc
    19981 caaacgccga ggcctactac gccgacgcca tcaccctgcc gctttatgca tcgatgagcg
    20041 acgcgcagca ggatcgcgtc gtcgacacgc tgcgcggcat gctgaagtga gcacagcgat
    20101 cgtggtgcag gcgcgcatgg gctcgagccg cctgcccggc aaggtgttga aggacatcgc
    20161 gggccgcccg atgctctcct accaaatgga gcggctgcgc cgcgtgaagc gcgccgagcg
    20221 catcgtcgtt gccaccaccg atcaaccggc cgacgatgcc gtcgagcgct tttgccagaa
    20281 ggagaaaatc gcctgcgtgc gcggctcgga gcacgacgtt cttgcgcgct accacctcgc
    20341 gatcgagcgg ttcccggctg acgtcgtggt gcggatcacc gccgactgcc cgctcatcga
    20401 cccggcgatc gtggacgagg cgatcgcggc ctacgaaccg gactacgttt cgaacatgct
    20461 cgagactacc tacccctacg gcatggcggt cgaggtgttc tccgcgcagg ctttgcgcga
    20521 agcgcatcgc gaggcgaaag atcccgccga gcgcgagcat gtcacgccgt tcatctaccg
    20581 gcacccggaa cgctatcgac tgagatcgct caccatggcg ccgaacctga gccaccaccg
    20641 ttggacggtc gatacgcccg aggacttcga gctggtgtcg cgcctcctga aaaccctcaa
    20701 accccatttc acgttgcagg atgtactcgc ggtcctggac aagcatccgg actggtgcgc
    20761 gatcaacgcg cacgtcgagc aaaaggcgct ctgatgggct acaagaccga gcaggaagag
    20821 ttctgggcgg gcgagttcgg caccgattac atccagcgca acacggggcc ggagctcctc
    20881 gcgtcgaacc tgggtttttt ctgcaaagcg ctggctcgcg ccgcgcggcc cgcgagctgc
    20941 atcgagttcg gcgcgaacat cggcatgaac ctgagggcgt tgaagctgct ttacccgtcg
    21001 ctcgagcagc acgccattga gatcaacgcc accgcggcga aggaactcgc gacctttctg
    21061 ccgccggcga acgtacacca ggcctcgatc ctcgacttca agcccacgcg cacatgggac
    21121 ctggtgctca tcaagggcgt gctgatccac atgaatcccg aatcgctgcc cgcggtgtac
    21181 gaggcgctcc acaaggcgac gggccgttac ctgctggtgt gcgagtacta caaccccgcg
    21241 cctgtgacgg tgacctaccg cggccacacc aaccggcttt tcaagcgcga tttctgcggc
    21301 gagatcatga cgcgcttccc cgacctgcag cttgccgact acggcttcgc gtaccggcgc
    21361 gacccgagct tcccgcagga cgacatcacc tggtttctga tggagaagca ctgagatgct
    21421 gaggtactgc aagcgctgtg tcatgccgga caccaagccg gacctgcggc tcgacgacga
    21481 cggcatctgc aacgcgtgcc gcagctacga gcggcgcgca acgatcgact gggcggcgcg
    21541 gggcagagag ctcgagtcaa tcctcaagaa ataccgcaac ccgagcggca agcactggga
    21601 ctgcatcatt ccggtctcgg gcggcaaaga cagcaccacg cagaccgtcc ggctcctgca
    21661 gctcggcatg aatccgctct gcgtcacggc aaccacgtgt gacctctcgg cgatcgggcg
    21721 caagaacatc gagaacatca agcgcctcgg cgtggattac atggagttct cgcccaatcc
    21781 ggtggtgcgc gccaagctga accgcatcgg gctgacccaa gtgggcgaca tttcctggcc
    21841 cgagcacgtc ggcatcttca ccatcccggt gcgcgcggcg gtgcagttca acatcccgct
    21901 catcatctgg ggcgagaact cgcagaacga gtacggcggc ccggcggccg cggccgagaa
    21961 caacacgctc aatcgccgct ggctggaaga gttcggcggt ttgctcgggc tgcgcgtgtc
    22021 ggacctcgtc ggcatggaag gcctcgagca ccggcacctc attccctacc agtacccgag
    22081 cgacgaggag ctgaaccgcg tcgcggtcac cggcctattc ctcggctact acctgccgtg
    22141 ggacggactt tccaacgcgc tcgtcgcgca ggccaacggc ttcacgacct tcgaccgcgc
    22201 catcgagggc tcgatggtca actacgagaa cctcgacaac catcagaccg gcatccacga
    22261 ctacttcaag tttttgaagt ttggttttgc gcgcgccacc gatatcgcct gcctgcacgt
    22321 gcgccgcggc cgcatcacgc gcgaggacgc gctcgacatc gtgcgccggc gcgacggcgc
    22381 tttcccgtgg acctacctcg gcaagccgct cgccgagatc ctcgcgccgc tcgacatgag
    22441 cgtcgatgag ttcgtgcgca tctgcgacaa gttcaccaac aagaagatct tcaagcgcga
    22501 cgccaacggc cagctcctca aggaccgctt tggcaacctc accaaggtga actacgacaa
    22561 cgcatgaagg tcgcggtgat cgactacggc ctgggcaacc tcggctcggt gcgccgcgcg
    22621 atcgccgagc tcggcgccga accggtcctc gccgacaagc ccgagcagct tgcggcggcc
    22681 gaccgcgtca tcttgccggg cgtcggcagc tttgccgatg gcatggtgct tctgcgcgag
    22741 cgcgggtggg tcggcgctct tcgcgagacg cgaaagcccg tgcttggcat ttgcctcggc
    22801 atgcaactcc tcgcttcgcg cggcacggag ggcggcgtca ccgaagggct cgatctcatt
    22861 cccggcgaag tggtcgccct cgagacgctc ggctgcgagc tgcgcattcc gcacgtcggc
    22921 tggaacgcgc tgaccgtgag ccacccctcg ggcatcgtgc gaggcattcc ggacgggacc
    22981 gacttctact tcgtccatag tttcgtcttc cagcccgggg aggcaacgga cgtcgtcgcc
    23041 accacgtcgt acggcgtgcc ggtgacggcc gtggtcgggc gcggcaacgt gatgggcacg
    23101 cagttccacc cggaaaagag ctcgaaggcg ggctttcggg tgctgcgcaa cttcctcgat
    23161 gccggcgcgt gctgaaggcc cgcatcattc ccaccctcct ctggaagaac gtcgggctcg
    23221 tcaagggcat cggcttcgac agctggcgcc gcgtcgggcc ggtcctgccc gcgatcaagg
    23281 tctacaacag ccgcgacgtc gatgagctgg tgcttgtcga catcaccgca agccacgaca
    23341 agaccgagcc cgactgggaa tcggtgcacg acttcgccga cgaatgcttc gtgccgctca
    23401 ccgtgggcgg cggcatcacc aacgtcgagc acatccgcca gctcctgcgc gccggggctg
    23461 acaaggtgag catcaacacc gccgcctatg ccaatccgaa gctcgtcgaa gacgcggcgc
    23521 gccgcttcgg ctcgcagtgc gtggtggcga gcatcgacgc acgacgcgcg gacgacgggc
    23581 gttatatctg ctacagccac tccggcacgg tcgccaccgg gcgagcgccg gacgactggg
    23641 cgcgcgagct cgaagcacgc ggcgcgggcg agattctgct cacctcgatc gagcgcgacg
    23701 gcaccatgca gggctacgac ctcgagctca tcgcttcggt cgtgcacgcg gtgaacgttc
    23761 cggtcatcgc ttccggcggc gcc
//
LOCUS       DASTPD010000028         6593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_826596, whole genome shotgun sequence.
ACCESSION   DASTPD010000028 DASTPD010000000
VERSION     DASTPD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826596"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFR57_01345"
     CDS             <1..659
                     /locus_tag="VFR57_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258050.1"
                     /translation="KLPYDIERDLQPVALVTTGYMLLAASPKAPFSTVRELIDYAKRN
                     PGKLFNASSSNGSPGHVSGELFKFMTGTEIVHIPYKGGAPAIADLIGGQVQLMFESTN
                     SIAPHVKAGRVRALAVTGEKRAISLPEVPTLMEAGVPGYEVNAWSGLITTAGVPRPVL
                     DKLNAAANRAIQQPETKERLAQLGSEGGGGTPEQFAALIRRDSAKWAEVVRRSGAKID
                     "
     gene            complement(676..1623)
                     /locus_tag="VFR57_01350"
     CDS             complement(676..1623)
                     /locus_tag="VFR57_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258051.1"
                     /translation="MRVLLMFLSLLSASAFAQYPNKPVRVLVGYAAGSSTDIVGRVMA
                     DRLSAYWKQSVYVETRAGAAGNLAADAAAKAAGDGYTLLFAQNGLAISAAALPNLPYR
                     AETDLVPLAPVAATPHILIVAPDFPAKSVRELVAKAKAEPGKLSFASSGVGNSDHLCG
                     ELFNLMAGVQAIHVPYKGGAPAAVDVMGGRIAYYFAGMPVGLPLAKSGKARALAVTSK
                     TRFAGAPDIPTVAEQGLPEYEATLWQGFFAPASVPAELAARIAADIEKVVALPETREK
                     LQGAGVSLFSSNHAEFKRFFPAEVAKWRGVVKKANLKLE"
     gene            complement(1626..2540)
                     /locus_tag="VFR57_01355"
     CDS             complement(1626..2540)
                     /locus_tag="VFR57_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0258052.1"
                     /translation="MVNPFQEIKKIPVERWSALPAKFRKKGRTLWSDPNRQLAAVDSF
                     LEGPSFDRDGRLWCVDIPFGRVFRIERNLEWELIVQYDGWPNGLKIHKNGEIYIADYR
                     KGLLKLDPKSGKIEPFLETAFSESFKGLNDLHFADNGDLYFTDQGQTGIGDPTGRVWR
                     LRTTGELQRIVQNAPSPNGITLNTKNTQIYVAVTRAQQIWRLPLMAGGTPSKTGVAIQ
                     LSGGHAGPDGIEMDSEDGLVVCHLGVGVWRFDSNCLPTHLVHAGADHRLMTNIAFRGK
                     TLYITDSLNGEILTAEMPVAGKDLFSMR"
     gene            2591..3166
                     /locus_tag="VFR57_01360"
     CDS             2591..3166
                     /locus_tag="VFR57_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258053.1"
                     /translation="MNRFALVSLLALFSVSAFGQTPTRVRGTITGLDGDILSVKSREG
                     KDLKLHLAPNVAVTTARKSSLEELKGKYVGVTAISKAGKMTALEVHAIPPQAKPGHFP
                     WDLQPESTMTNANLDGIAQTSGGNEITLNYQGGSQKVLVPPGTPIVAFDPGARADLKP
                     GEWIWTNARQEGDKIVVERLNVSKDGVKPPH"
     gene            3222..3698
                     /locus_tag="VFR57_01365"
     CDS             3222..3698
                     /locus_tag="VFR57_01365"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEU0258054.1"
                     /translation="MRGTITAVDGNAITVNGKERVLISEKTEIVFARPISLADIKPGD
                     FLGVTSQKRPDGALVAQEVRRFPKPLNPGHRPFDGRDDQTMTNASVDAVVSATSGREL
                     TMAYPGGTQKIVVPESASVSMLVPGERAQLVRGAPVNLTMDASGVALRIQAGAAPP"
     gene            3739..4305
                     /locus_tag="VFR57_01370"
     CDS             3739..4305
                     /locus_tag="VFR57_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU0258055.1"
                     /translation="MKKSSAVLVGIATFAALPVLAHDGGNRFGGNLDGYQEVAVAPQP
                     STISTTGNGRFDLLIDERRGEVHWQLSYGQLESAVTQAHIHLGLPAIAGGISVFLCTN
                     LGNGPAAPAQSPQGCPQAVGADTVTISGTFTRDDVIGPAGQGIAAGEFAELLRAIRAG
                     ATYANVHTQNRPAGEIRAQLQRGHSKGN"
     gene            complement(4399..5865)
                     /gene="tcuA"
                     /locus_tag="VFR57_01375"
     CDS             complement(4399..5865)
                     /gene="tcuA"
                     /locus_tag="VFR57_01375"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEU0258056.1"
                     /translation="MSYDVIVVGGGNAALCAALSASETVKKVLVLERAPEEESGGNSR
                     FTAGLFRLVYTGPEDLKTLIPDLSAAEIANTDFGTYTEEQFLDDMARLTEYRCDPDLT
                     EILVKQSLATAQWMRKRGVRFTAAWGRQAFKIGGKFKFWGGLTVEAVGGGPGLVESET
                     QLAKKAGIEIWYGARAVSLLADDDGVHGVVVKHQGRTKELKSRAVVLAAGGFQANTEW
                     RTRYMGPGWELAKVRGTRFNTGDAIRMALDVGAAAHGNWSGCHAVAWDRNAPETGDLA
                     VGDGFQKHSYPWGVYINAEGKRFVDEGADFRNYTYAKYGRVILQQPKQFAWQVFDAKM
                     KPQLRDEYRIKQVTKVSGNTLEELVSRMDDTDQKTALQTLKKYNAAVRTDIPFNPNVK
                     DGRRTEGLEVNKSNWANTLDTPPFEAYAVTCGITFSFGGLKINTDAQVINSDGEPIPG
                     LYAAGELVGGIFWFNYPGGSGLTNGAVFGRLAGRNAAR"
     gene            complement(5865..>6593)
                     /locus_tag="VFR57_01380"
     CDS             complement(5865..>6593)
                     /locus_tag="VFR57_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258057.1"
                     /translation="RADPDGYTLLITHIGSLALNPFLYASLPFDVNRDFVHISLLAKV
                     PIFVVHESVPAKDAREFVALAKKEPGKFYYGSAGNGSAGHLAMEYFKLVAGIELQHVP
                     YKGTGPNLVDLVAGRTHATSAGTPPLMPHVKAGKLRVIGVGSPKRLPSLPESPTIAEQ
                     GFAGFETSQWYGLSAPAKTPAAVIKRLADEAAKAARSPAVVERYKADDAEAVGSTPQE
                     YTDFIAKEQARWKDVIQRANIKID"
BASE COUNT         1145 a         2161 c         2153 g         1134 t
ORIGIN      
        1 cgaagctgcc ctacgacatc gagcgcgacc tccagccggt ggcgctggtg accaccggct
       61 acatgctgct cgccgcctcg ccgaaggcgc ccttcagcac ggtcagggag ttgatcgact
      121 acgcgaagcg gaaccccggc aagctgttca acgcgtcctc gagcaacggc tcgcccggcc
      181 acgtcagcgg cgagctcttc aagttcatga ccggcaccga gatcgtgcac atcccgtaca
      241 agggcggcgc gccggcgatc gccgacctga tcggcggcca ggtgcagctc atgttcgaga
      301 gcaccaactc gatcgcgccg cacgtgaagg ccgggcgcgt gcgcgcgctc gcggtcaccg
      361 gcgagaagcg cgccatcagc ctgcccgaag tgccgacgct gatggaagcc ggcgtgcccg
      421 gctacgaagt gaacgcgtgg agcggcctca tcaccaccgc cggcgtgccg cgaccggtgc
      481 tcgacaagct gaacgcggcg gccaaccgcg cgatccagca gcccgaaacg aaagagcgcc
      541 tcgcgcagct cggcagcgaa ggcggcggcg gcacgcccga acaatttgcg gcgctcatcc
      601 gccgtgattc ggcgaaatgg gccgaagtcg tgcgccgctc cggcgccaag atcgactgaa
      661 agggtcaaga cccttttatt cgagtttcag gtttgctttc ttgacgacgc cgcgccactt
      721 cgcgacttcg gcggggaaga agcgcttgaa ctcggcgtga ttcgacgaga aaagcgaaac
      781 gccggcgcct tgaagctttt cccgtgtttc cggaagcgcg acgacctttt cgatgtcggc
      841 ggcgatgcga gcagcgagct ccgctggcac ggaagccggc gcgaagaagc cctgccatag
      901 cgtcgcctcg tattccggca gcccctgctc cgccaccgtc ggaatatcgg gggcgccggc
      961 aaaccgcgtc ttgctcgtga cggcgagcgc gcgcgctttg ccggacttcg cgagcggcag
     1021 gccgaccggc atgccagcga agtaataggc gatgcggcca cccatcacgt cgaccgccgc
     1081 gggcgcgccg cccttgtacg gcacgtggat cgcctgcacg cccgccatga ggttgaagag
     1141 ctcgccgcag aggtgatcgg agttgccgac cccagacgac gcgaacgaaa gcttgcccgg
     1201 ctccgccttc gctttcgcga cgagctcgcg cacgctcttc gccgggaaat cgggcgcgac
     1261 gatgagaatg tgcggcgtcg ccgcgaccgg ggcgagcggc accaggtcgg tctcggcgcg
     1321 gtacggcaga ttgggaagcg cggccgcgct gatcgcaagg ccgttctgcg cgaagagcag
     1381 cgtgtagcca tcgcccgccg ctttcgccgc cgcatcggcc gcgaggttgc ccgcggcgcc
     1441 cgcgcgcgtt tccacataca cgctctgctt ccagtacgcg gagagccgat cggccattac
     1501 cctgccaacg atgtcggtcg agctgccggc cgcgtagccg accagcacgc gcaccggctt
     1561 gttgggatat tgcgcgaacg cgctcgcaga aagcagactt agaaacatca gaaggacgcg
     1621 catgcctacc tcatcgagaa gagatccttg cccgcgaccg gcatctccgc ggtgaggatc
     1681 tcgccgttca gggaatcggt gatgtaaagc gtcttgccgc ggaaggcgat gttggtcatg
     1741 agccgatggt ccgcgcctgc gtgcacgaga tgcgtgggca ggcagttcga atcgaagcgc
     1801 cagactccca cgccgagatg gcacacaacc aggccgtctt ccgagtccat ctcgattccg
     1861 tccgggccgg cgtgcccgcc ggagagctgg atcgccacac cggtcttgga cggcgtgccg
     1921 ccggccatga ggggaagccg ccagatctgc tgcgcgcgcg tcacggcgac gtagatctgc
     1981 gtgtttttcg tgttcagcgt gatgccgttc ggcgaaggcg cgttctgcac gatgcgctgc
     2041 agctcgcccg tggtgcgcaa gcgccacacg cggccggtcg gatcgccgat gccggtctga
     2101 ccctggtcgg tgaaatacag gtcaccgttg tcggcgaagt gcagatcgtt caggcccttg
     2161 aagctttcgc tgaacgcggt ctcgaggaag ggctcgatct tcccgctttt cgggtcgagc
     2221 ttgagcaggc ccttcctgta atcggcgatg tagatttcgc cgttcttgtg gatcttgagg
     2281 ccgttcggcc agccgtcgta ctgaacgata agttcccact cgagattgcg ctcgatgcgg
     2341 aagacacgcc cgaaagggat gtccacgcac cacaggcgtc cgtcgcgatc gaacgacggt
     2401 ccctccagaa agctgtcgac ggccgcaagc tgccggttcg gatccgacca gagcgtgcgc
     2461 cccttcttgc ggaacttcgc cggcagcgcg ctccagcgct cgaccggaat tttttttatt
     2521 tcctggaaag ggttgaccat ggaatgcggc gaaggatagg gatgttcaca gggtcggaca
     2581 aggaggcgtc atgaaccgct tcgccctcgt ttcgctgctc gccctgttca gcgtgagcgc
     2641 cttcggccag accccgaccc gtgtgcgcgg caccatcacc gggctcgacg gcgacatcct
     2701 ttccgtcaag tcccgcgagg gtaaggacct caagctgcat ctcgccccga atgtcgccgt
     2761 caccacggcg cgcaagagct cgctcgagga gctcaaaggc aagtacgtcg gcgtcaccgc
     2821 gatcagcaag gccggcaaga tgaccgcgct cgaggtccac gcgatcccgc cgcaggcgaa
     2881 gcccgggcat tttccgtggg acctgcagcc ggaatcgacc atgaccaacg cgaacctcga
     2941 cggcatcgcg cagacgagcg gcggcaacga gatcacgctc aactaccagg gcggatcgca
     3001 aaaggtgctg gtcccgccgg gcacgccgat cgtcgccttc gatccaggcg cgcgcgccga
     3061 cctgaagccc ggcgagtgga tctggaccaa cgcgcgccag gaaggcgaca agatcgtggt
     3121 cgaacgcctg aacgtcagca aagatggcgt caagccgcct cactaagctt gtctttcttg
     3181 cactcgtgtt cgcggcgagt tgcgcatcgg cgcaaacgcg cgtgcgcgga acgatcacgg
     3241 cggttgacgg taacgccatc accgtgaacg gcaaggaacg ggtgctgatc agcgaaaaaa
     3301 cggagatcgt gttcgccagg ccgatttcgc tcgcggacat caagccgggc gattttctgg
     3361 gcgtcacttc gcagaaacgt cccgacggcg ctctcgtcgc ccaggaagtg cgccgctttc
     3421 ccaagccgct aaaccccggc caccggccct tcgatggccg cgacgatcag acgatgacga
     3481 acgcctcggt cgatgcagtg gtgagcgcga cgagcggcag agaattgacc atggcgtatc
     3541 cgggcggcac gcaaaagatc gtcgttccgg aaagcgcgtc tgtttcgatg ctcgttcccg
     3601 gcgagcgcgc ccagctcgtg cgcggcgcgc cggtcaatct gacgatggac gcgagcggcg
     3661 ttgcgcttcg cattcaagcg ggcgcggcgc cgccgtgatc gcgtgtaaag gcagcagcat
     3721 tcaatagagg aggaagcaat gaagaaatcg agcgcagtac ttgtcggcat cgccaccttt
     3781 gccgcgttgc cagtcctggc gcacgacggc ggcaatcgct tcgggggcaa cctcgatggg
     3841 tatcaggaag tggccgtcgc gccacagcca agcacgatct caacgaccgg taacggccgc
     3901 tttgacctcc tcattgacga gcgtcgcggg gaggtgcatt ggcagctcag ctatggccaa
     3961 ctcgaaagcg cagtaacgca agcccatatt cacctcgggc tcccggccat cgccggcgga
     4021 atcagcgtct tcctgtgcac caaccttggg aatggcccgg cggcgcctgc gcaatcgccg
     4081 cagggatgcc cgcaggcggt cggcgcggac acggtgacca tcagcggcac gttcacaaga
     4141 gacgacgtca tcggtccggc gggccagggg atcgctgcag gcgaattcgc tgagctgcta
     4201 cgagccattc gcgccggcgc gacgtacgcg aatgttcaca ctcagaatcg gccggccggc
     4261 gagattcgtg cgcagctgca gcgcggccac agcaagggga actagagcgg ggcgacgcct
     4321 tttacacccc agcctcgggc ctcgggaaag cctgcgcgtc gcggacttcg aatgtcgccg
     4381 cggaccagac gtctttcttc accgggccgc gttacgcccc gcgaggcggc cgaatacagc
     4441 accgttcgtc agtcctgaac cgcctgggta gttgaaccag aaaatcccgc cgaccagctc
     4501 gcctgcggcg tagaggccgg gaatcggttc gccgtcggaa ttgatgacct gcgcgtcggt
     4561 gttgatcttc aggccgccga agctgaaggt gatgccgcag gtgaccgcgt acgcctcgaa
     4621 cggcggcgtg tcgagcgtgt tggcccaatt gctcttgttg acttcgagcc cctcggtgcg
     4681 ccggccgtcc ttcacgttcg ggttgaacgg gatgtcggtg cgcaccgcgg cgttgtactt
     4741 cttcagcgtc tgcaacgcgg tcttctgatc ggtgtcatcc atgcgcgaaa cgagctcttc
     4801 gagcgtgtta ccgctcacct tcgtcacctg cttgatgcgg tactcgtcgc gcagctgcgg
     4861 cttcatcttc gcgtcgaaga cctgccaggc gaactgcttg ggctgctgca ggatcacgcg
     4921 accatacttg gcgtacgtgt agttgcggaa atccgcaccc tcgtcgacga agcgcttgcc
     4981 ctcggcgttg atgtacacgc cccaggggta ggagtgcttc tggaagccgt cgccgacggc
     5041 gaggtcgccc gtctccgggg cgtttcgatc ccacgccacc gcgtggcagc ccgaccagtt
     5101 tccgtgcgcc gccgcgccga cgtcgagcgc catgcggatc gcgtcgccgg tgttgaagcg
     5161 cgtgccacgc actttggcga gctcccagcc cggtcccatg tagcgcgtgc gccactcggt
     5221 gttcgcctgg aagccgccgg ccgcgagcac gaccgctctt gatttcagct ccttcgttct
     5281 tccttggtgc ttcacgacga cgccatgcac gccgtcgtcg tccgcaagaa gagacactgc
     5341 gcgcgcgccg taccagatct cgatgcccgc tttcttcgcg agctgggtct cggactcgac
     5401 gagtccaggg ccgccgccga ccgcctccac ggtgagtcca ccccagaact tgaacttgcc
     5461 gccgatcttg aacgcctgcc ggccccacgc cgcggtgaag cgcacgccgc gctttcgcat
     5521 ccactgcgcg gtggcaagac tctgcttcac caggatctcg gtgaggtccg gatcgcagcg
     5581 gtactcggtg aggcgcgcca tgtcgtcgag gaactgctct tcggtgtacg tgccgaagtc
     5641 ggtgttggcg atttcggcgg ccgaaaggtc gggaatcagc gtcttgaggt cttcggggcc
     5701 ggtgtagacc aggcggaaaa gcccggccgt gaagcgcgag ttgccgccgg attcctcctc
     5761 cggcgcccgc tcgagaacca agactttctt tacggtttca gacgccgaca gtgccgcgca
     5821 cagcgcggcg ttgccgccgc cgacgacgat gacgtcgtaa ctcatcagtc gatcttgatg
     5881 ttggcgcgct ggatcacgtc cttccagcga gcctgttctt tggcgatgaa atcggtgtat
     5941 tcctgcggcg tggagccgac ggcctcggcg tcgtcggctt tgtagcgctc aacgaccgcc
     6001 gggctccgcg cggcctttgc tgcctcgtcg gcgaggcgct tgatcaccgc agccggcgtc
     6061 ttcgcaggcg ccgacaaccc gtaccactgc gaagtctcga atcccgcgaa gccctgctcg
     6121 gcgatggtgg gcgattccgg cagcgagggc aggcgcttgg gcgagccgac gccgatgacg
     6181 cgcagcttgc cggctttcac gtgcggcatg agcggcggtg tgccggccga cgtggcgtgc
     6241 gtgcgtcccg caacgaggtc gacgagattc gggcccgtgc ccttgtacgg gacgtgctgt
     6301 agctcgatgc cggccacgag cttgaagtac tccatggcga ggtggccggc gctgccgttg
     6361 ccggccgaac cgtaatagaa cttgcccggc tccttcttcg cgagggcgac gaactcgcgc
     6421 gcgtccttcg cgggcacgct ctcgtgcacg acgaagatcg gcaccttggc gagcagcgag
     6481 atatgcacga agtcgcggtt cacgtcgaac ggcaggctcg cgtagagaaa cggattgagc
     6541 gcgaggctgc cgatgtgcgt gatgagaagc gtgtagccgt ccggatcggc ccg
//
LOCUS       DASTPD010000029         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_10458, whole genome shotgun sequence.
ACCESSION   DASTPD010000029 DASTPD010000000
VERSION     DASTPD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10458"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFR57_01385"
     CDS             complement(<1..486)
                     /locus_tag="VFR57_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0258058.1"
                     /translation="MNAPHSPSPLASVPMAPADPILGVTEAFAADKNPSKVNLGVGVY
                     TDDNGKIPLLKCVRRAEELRMRTSPHRGYLPIDGLATYDIAVQRLVFSKDSKEIVTVQ
                     ALGGTGGLKIGADFLKQINPSAQVWISEPSWENHRQLFEAAGFTVKSYPYYEPKTRGL
                     DF"
     gene            531..2525
                     /gene="uvrB"
                     /locus_tag="VFR57_01390"
                     /pseudo
     CDS             531..2525
                     /gene="uvrB"
                     /locus_tag="VFR57_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
     gene            2563..3081
                     /locus_tag="VFR57_01395"
     CDS             2563..3081
                     /locus_tag="VFR57_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEU0258059.1"
                     /translation="MLWKLRLLWYRTDKGALAFALFLALIAGSLALALTLVHAERDAQ
                     GARVRDFHARSVDCLARNVYYEARGESLTGQYAVAEVTMNRKAHPRYPSTVCEVVYQK
                     DAFSWTSLGPLDPPAGEPWQRAQRIAEEVYYQRRPAKMPGVLHFHATYVKPDWSKERQ
                     RVSRIGRHVFYR"
     gene            complement(3124..3276)
                     /locus_tag="VFR57_01400"
     CDS             complement(3124..3276)
                     /locus_tag="VFR57_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU0258060.1"
                     /translation="MGWYRDLRRYGTVPHCGFGLGFERTVAYATGLTNVRDAIPFPRT
                     PGNARY"
BASE COUNT          601 a         1037 c         1099 g          548 t
ORIGIN      
        1 gaagtcgagc ccgcgagttt tcggctcgta atagggatag ctcttcaccg tgaacccggc
       61 cgcttcgaaa agctggcggt ggttctccca gctcggctcg ctaatccaga cctgcgcgct
      121 cgggttgatc tgcttgagaa agtcggcgcc gatcttcagc ccgccggttc caccgagcgc
      181 ctgcacggtg acgatctcct tcgaatcctt gctgaaaacc agccgttgca cggcgatgtc
      241 gtacgtcgcc aggccgtcga tgggcaggta gccgcggtgc ggcgaggtcc tcatccgcag
      301 ctcctcggcc cgccttacgc acttgaggag cgggatctta ccgttgtcgt cggtgtatac
      361 gccgacgccg aggttgacct tcgacgggtt cttgtcggcg gcgaaagctt cggtgacgcc
      421 gaggatcgga tcggccggcg ccatcggcac cgaggcgaga ggagagggag agtgcggcgc
      481 gttcattgga ctaaaatggt gggtttcgca cgcgcattct acctgtggca ttcaagctag
      541 aagagctttt tccgccgtcc ggcgatcagc cggaggcgat cgcgaagctc ctcgagggta
      601 tcgaggacgg gctttccttc cagacgctcc tcggggtcac gggctcggga aagacctaca
      661 ccatggcgaa cgtgatcgcg cgcctcggcc ggccggcgct ggtgctcgcc ccgaacaaga
      721 cgctcgccgc gcagctttat gccgagttcc gggagttctt cccggaaaac gcggtggagt
      781 acttcgtttc gtactacgac tactaccagc cggaagcgta tgtcccggcg cgcgacctct
      841 atatcgagaa ggactcgtcg atcaacgagc acatcgagca aatgcggctg tcggcgacga
      901 agtcgctcct cgagcggcgc gacgtagtga tcgtggcgac ggtgtcagcg atctacggca
      961 tcggcgaccc gtcggagtac cacggcatga tccttcacct ccggcacgga gagagggtgt
     1021 cgcaacgcga cgttctggcg cggctggtgg cgatgcagta ccagcggagc gagctcgatt
     1081 tccggcgcgg caccttccgt gtacgcggcg acgtgatcga cgtattccca gccgaacacg
     1141 ccgagcaggc gatcaggatc tcgctgttcg acgacgagat cgagaagctc gaggtgttcg
     1201 atccgctcac cggccgggtt catcacccga tggcgcgctt tacggtgtac ccgtcaagcc
     1261 attacgtgac gccgcgcgac accacgctgc gagcgatcga ggcgatcaag gaggagctcc
     1321 gcgaccgcat cgagttcttc cagaaggccg ggcgcctggt ggaagcgcag cgcatcgagc
     1381 agcgcacgcg atttgacctc gagatgctgg ccgagctcgg gttctgcaag ggaatcgaaa
     1441 actactcgcg tcacctgtcg ggccgccggc ccggcgagcc gccgcccacg ctcatcgact
     1501 acctgccgca ggacgcgctc atgatcgtcg atgagagcca cgtcacgatc ccgcagctcg
     1561 gcgggatgta ccgtggcgac cgctcgcgca aggaaaacct cgtcgagtac gggttccggc
     1621 tgccgtcggc gctcgacaac cggcccctgc gcttcgacga gttcgagaac gtggtgcgcc
     1681 agacgatctt cgtgtcggcc acgccggcgg aatacgagcg cgaaaagtca ggcaccgcga
     1741 tcgtcgagca ggtggtccgg ccgaccgggt tgatcgaccc cgaggtgatc gtgcggccgg
     1801 cgtcgacgca ggtcgacgat ttattgtccg aaataggaga aagaacccaa cgatccgagc
     1861 gcgtcctcgt gacgactctg acgaagcgca tggccgagga gctgaccgaa tacctcgccg
     1921 agcacggggt gaaggtgcgc tacctgcact cggaggtgga aacggtggag cgggtggaaa
     1981 tcatccgcga cctgcgcctg ggcgagttcg acgtgctggt cggcatcaac ctgctgcgcg
     2041 agggcctcga cctgccggaa gtgtcgctgg tcgcgatcct ggatgccgac aaggagggct
     2101 tcctgcgctc ggagcgctcg ctcatccaga ccatcggccg tgccgcgcgc cacatcaacg
     2161 gcacggcaat cctgtacgcg gaccgggtca ccgagtcgat ccagaaagca ttggcggaaa
     2221 ccgagcggcg ccggcgcaag cagcgcgcgt acaacgagcg ccaccgcatt acgccgaagg
     2281 gcgtggtgaa gcgcatcaag gacctgatcg acggcgtata cgacgcgacc gaggcccggg
     2341 ccgcccagga cgaggcgcgc tacgaggcca tgtcggagaa gcagatcgcg cgcgagatca
     2401 agctgctcga gaagaagatg ctgcagcacg ccaagaacct cgagttcgag caggctgccg
     2461 cagcgcggga ccgcctagcg gaactgaaaa aaagggtttt cggtatcgag gccgcggcaa
     2521 tttgaccttc gcccgcaggc gccctgcggg cagagtgtcg ccatgctgtg gaaactgcgc
     2581 ctgctgtggt accggaccga caagggcgcg ctcgcctttg cgctgttcct ggcgctcatc
     2641 gctgggtcgc ttgcactcgc gctgaccctg gtgcacgcgg agcgcgatgc gcagggcgcg
     2701 cgcgtgcgcg acttccacgc tcgcagcgtc gactgcctgg cgcgcaacgt gtactacgag
     2761 gcgcgcggcg aatccctcac cggacagtac gcagtggccg aagtcaccat gaaccggaag
     2821 gcgcaccctc gctatccgag cacggtctgc gaggtcgtgt accagaaaga cgcgttttcc
     2881 tggacctcgc tggggccgct tgatccgccc gcgggggagc cgtggcagcg tgcgcaacgg
     2941 atcgccgagg aggtgtacta ccagcgccgc ccggccaaaa tgcccggtgt gctgcacttc
     3001 cacgccactt acgtgaagcc cgactggtct aaggagaggc agcgcgtttc ccgcattggg
     3061 cggcacgtgt tctaccggta aaccggattc ccggcttgag gccctcgaag acgacgcgtg
     3121 gctttagtac ctcgcgttcc cgggcgtcct cgggaacgga atcgcgtcgc gcacgtttgt
     3181 gaggccggtg gcgtaggcga ccgtgcgctc gaagccgagg ccgaacccgc agtggggcac
     3241 ggtgccgtaa cggcgaaggt cgcgatacca gcccaggttg tcctt
//
LOCUS       DASTPD010000030         4220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_419793, whole genome shotgun sequence.
ACCESSION   DASTPD010000030 DASTPD010000000
VERSION     DASTPD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419793"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFR57_01405"
     CDS             <1..232
                     /locus_tag="VFR57_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258061.1"
                     /translation="VIVIGGIGSVRGALVGALIVGVVDTLGRAFLKPLLSAMMSPTAG
                     EAAGPALASMLIYLLMAVVLAVKPAGLFPARG"
     gene            232..1164
                     /locus_tag="VFR57_01410"
     CDS             232..1164
                     /locus_tag="VFR57_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258062.1"
                     /translation="MNTRLVLWGISVAVLVALPPLVVAIGQPFYIDLVRRIMILAIAA
                     VSLNLILGYGGLVSFGHAAYLGIGAYAVGILGFYGVTSGWLQWGMAIGASALVALAIG
                     AVSIRTSGIYFIMITLAFTQMLYYLGISLDVFGGDDGMRLKAKSQFSGLIDLGNPIAF
                     YYLALVLMLVSVFLVYRIVNSKFGMVLRAAKSNEARARAIGFSPYPYRLAAFVIAGAM
                     CGLAGALYANHTNYIVPGLMSWQQSGDIMFMVILGGMASTAGPVLGTFVLLILEEILK
                     GWTEHWQVVLGPLLVLSVIFFRRGLAGIFYKPDA"
     gene            1157..1888
                     /locus_tag="VFR57_01415"
     CDS             1157..1888
                     /locus_tag="VFR57_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258063.1"
                     /translation="MLEVRGLQKSFGALRACDGIDFDVAEGETHAVIGPNGAGKTTFI
                     SQLAGNLRPDAGVIRFAGEDVTRLPAHARARKGFARSFQITSVFPDFSALHNVALAVQ
                     AHLGGTGFWRPARSDPALTGPARELLREVGLGHREDALAANMSHGEHRQLEVAMALAT
                     KPRLLLLDEPMAGMGIEESQRMIELLARLKQEKTIILVEHDMDAVFRLADRISVLVYG
                     RVIATGEPESIRANEEVRKAYLGEE"
     gene            1888..2574
                     /locus_tag="VFR57_01420"
     CDS             1888..2574
                     /locus_tag="VFR57_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258064.1"
                     /translation="MLEIRSLETSYGRSQVLFGIDLQLGRSEVVSLLGRNGMGKTTTV
                     RSIMGITPPKSGTISFEGEAIQRLPSYRVAQKGIGLVPEGRQVFPNLTVRENLVATAR
                     GGAWTLERVFKLFPHLEARQANYGNQLSGGEQQMLAIARALMTNPRLLILDEATEGLA
                     PRIRGEIYRSIEELKAQGLSILVIDKDVKALTRVADRHYVLEKGRVVWSGSSRELAAD
                     TNVQHRYLGV"
     gene            2585..3568
                     /locus_tag="VFR57_01425"
     CDS             2585..3568
                     /locus_tag="VFR57_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0258065.1"
                     /translation="MKRENVIGRANVEDTPELEAYYRSLEAEALGALWTVANEIEPWY
                     PQPKSVPTLWRWKRIESHVRKAATLVSAEKAARRVVMLVNPGRKEWSAAVGLLYSGVQ
                     VMNPGEFTSAHRHQASALRFVMEGRGAYTVVDGERFTLGARDFVLTPNGTWHDHGVEA
                     GGTQCIWQDGLDIPLMNSLDANFYEVHPEVVQKAIPGGPEKDNWSKPYSPVFLYKWDD
                     SYKSLKRNGRVFEYQNPLTGGAVMPTMSARLELIKTNSEVKRHTGSVVYQVAAGSGWT
                     EMGDQRFEWEEKDIFCVPAWTRYRHGAHEESVLFSFNDFPAMRALALYREE"
     gene            3565..>4220
                     /locus_tag="VFR57_01430"
     CDS             3565..>4220
                     /locus_tag="VFR57_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0258066.1"
                     /translation="MISREQNELMTRVGPETPAGKLLRRYWQPVALVDELKGERPVRP
                     VKVLGESFVLFRDTEGKCGVIDRQCAHRGADLAYGRLEDGGLRCSFHGWLYDVAGKCL
                     ETPAEPEGSRLCEHIRLRSCPVVEKSGVLFAYLGAGEPPAFPQFDCFVAPDAYTFAFK
                     GYWDCNWLQALEVGIDPSHASWLHKFFEDEDPAASYGRQFRGTPADSRMPISKVLREY
                     "
BASE COUNT          734 a         1340 c         1401 g          745 t
ORIGIN      
        1 cgtgattgtc atcggcggta tcggctcggt gcgcggcgcg ctcgtcggcg cgcttatcgt
       61 cggcgtggtc gatacgctcg ggcgcgcatt cctcaagccg ctgctcagcg ccatgatgtc
      121 ccccacggcc ggcgaggccg cggggccggc gctcgcctcg atgctcatct accttctgat
      181 ggcggtggtg ctcgcggtca agcccgcggg cctcttcccg gcgcgcggat gatgaacacg
      241 cggctcgtcc tctggggaat ttcggtcgcg gtgctcgtag cgctgccgcc gctcgtcgtt
      301 gcgatcgggc agccgttcta catcgacctg gtgcgccgga tcatgatcct cgccattgcc
      361 gcggtaagcc tcaacctgat actcggctac ggcggcctgg tttcgttcgg ccatgccgct
      421 tatctcggca ttggcgccta cgcagtcggc atcctcggct tctatggggt gacgagcggc
      481 tggctgcaat ggggcatggc gatcggcgct tcggcgctcg tcgcgctcgc gatcggcgcg
      541 gtgtcgatca ggaccagcgg catttatttc atcatgatca cgcttgcatt cacccagatg
      601 ctctactacc tcggtatctc gctggacgtg ttcggcggcg acgacggcat gcggctcaag
      661 gcgaagagcc agttctccgg cttgatcgac ctcggcaacc cgatcgcgtt ttactacctg
      721 gcgctcgtgc tcatgctcgt atcggttttc ttggtttacc ggatcgtgaa ttcgaagttc
      781 ggcatggtgc tgcgcgccgc caagtcgaac gaggcgcgcg cgcgcgccat cgggttctcg
      841 ccctaccctt accggctcgc agccttcgtc atcgccggtg ccatgtgcgg gctcgccggg
      901 gcgctctacg ccaaccatac caactacatc gtcccgggcc tgatgagctg gcagcagtcc
      961 ggcgacatca tgttcatggt catcctgggc ggcatggcga gtaccgcggg cccggtgctc
     1021 ggcacgtttg tcctgctcat cttggaggag atcctcaagg gctggaccga gcactggcag
     1081 gtagtcctcg ggccgctgct cgtgctctcg gtcatttttt tcagacgcgg tctcgcgggg
     1141 atcttctaca agccggatgc ttgaagttcg gggattgcag aaaagcttcg gtgccttgcg
     1201 cgcttgcgac ggcatcgact tcgacgtggc cgagggcgag acgcacgcgg tgatcgggcc
     1261 caacggggcg ggcaagacga ccttcatcag ccagctcgcc ggcaacctgc gccccgatgc
     1321 cggcgtgatc cgcttcgccg gcgaggacgt aacccgcctc ccggcgcatg cgcgcgcgcg
     1381 caaaggcttc gcgcgctcgt tccagatcac cagcgtgttt cccgacttca gcgccctgca
     1441 caacgtcgcg ctcgccgttc aggcgcatct cggcggcacg ggattctggc gtcccgcgcg
     1501 cagcgaccca gcgctgaccg gaccggcgcg cgagcttctc cgcgaagtcg ggctcggcca
     1561 ccgcgaggat gcgttggccg ccaacatgtc gcacggcgag caccgtcagc tcgaggtggc
     1621 gatggcgctc gctacgaagc cgaggctgct cttgctcgac gaaccgatgg ccggcatggg
     1681 aatcgaggag tcgcagcgca tgatcgagct gctcgcgcgg ctcaagcagg agaagaccat
     1741 catcctggtc gagcacgaca tggacgccgt gttccgcctc gccgaccgaa tttccgtgct
     1801 ggtgtacgga cgcgtcatcg cgaccggcga gcccgagagc atcagggcga acgaagaggt
     1861 gcggaaagct tacctgggcg aagagtgatg ctggagatcc gcagcctcga gacttcctac
     1921 ggccgcagcc aggtgctctt cggcatcgac ctgcaactcg gaagaagcga agtcgtctcg
     1981 ctgctcgggc gcaacggcat gggcaagacg accaccgtgc gctccatcat gggcatcaca
     2041 ccgccgaagt ccggcacgat ttcgttcgaa ggcgaagcca tccagcgcct gccgtcctac
     2101 cgggtggcgc agaagggaat cggcctcgtc cccgagggac ggcaggtatt cccgaacctt
     2161 accgtacgcg aaaaccttgt ggccaccgcg cgcggcggcg cgtggacgct ggagcgcgtg
     2221 ttcaagctgt ttccgcacct cgaggcgcgc caggccaact acggcaacca gctctcgggc
     2281 ggcgagcagc agatgctcgc catcgcgcgc gcgctcatga cgaacccgcg cctcctgatc
     2341 ctcgacgagg cgaccgaagg actcgcgccg cgcatccgcg gcgagatcta tcgctcgatc
     2401 gaagagctga aggcgcaggg actttcgatc ctggtcatcg acaaggacgt gaaggcgcta
     2461 acccgggtgg cggaccgtca ttacgtgctg gaaaagggac gcgtggtgtg gagcggaagc
     2521 tcccgcgagc tcgcggccga cacgaacgta cagcaccgat atctcggggt gtagactttt
     2581 cggcatgaag cgcgaaaacg tcatcggccg ggcgaacgtc gaagacacgc ccgagcttga
     2641 agcctattac cgcagcctcg aggccgaagc gctcggcgcg ctatggaccg tggcgaacga
     2701 gatcgagccg tggtatccgc agcccaagtc ggtaccgacg ctctggcgtt ggaaaagaat
     2761 tgaatcccat gtaagaaaag cagcaaccct tgtgtcggcc gaaaaggcgg cgcggcgcgt
     2821 ggtcatgctg gtcaaccccg ggcgcaagga atggagcgcc gcggtgggcc tgctctacag
     2881 cggcgtgcag gtgatgaatc ccggcgagtt cacctccgcg caccgccacc aggcgagcgc
     2941 gctgcgcttc gtgatggagg gacgcggtgc gtacaccgtg gtggatggcg agcggttcac
     3001 gctcggcgcg cgcgatttcg tgctgacgcc gaacggcacc tggcacgacc acggcgtcga
     3061 ggccggtggc acgcagtgca tctggcagga cggcctcgac atcccgctca tgaactcgct
     3121 cgacgccaac ttctatgaag tccatccgga agtggttcaa aaagcgatcc ctggaggacc
     3181 ggagaaggac aattggtcaa agccctattc gccggtcttt ctatacaaat gggatgacag
     3241 ctacaagtcg ctcaagagaa atggaagggt ttttgagtac cagaacccgc tcaccggcgg
     3301 cgcggtaatg cccaccatga gtgcgcgcct cgaactaata aaaacgaact ctgaagtgaa
     3361 acgccatacc ggcagcgtcg tgtatcaggt cgctgcgggc agcggctgga cggagatggg
     3421 cgaccagcgc ttcgagtggg aggagaagga catcttctgc gtgccggcat ggacgcgcta
     3481 ccggcacggc gcgcatgagg agtcggtgct gttctccttc aacgacttcc cggcgatgcg
     3541 cgcgctcgcg ctgtaccgcg aggaatgatc agccgggagc agaacgagct catgacgcgg
     3601 gtcgggcctg agacgcccgc cggcaagttg ctgcgccgct attggcagcc ggtggcgctg
     3661 gtggatgaac tgaagggcga gcgcccggtg cggccggtaa aggtgttggg cgaaagcttc
     3721 gtccttttta gagacactga aggcaaatgc ggcgtgatcg atcggcaatg cgctcaccgc
     3781 ggcgcggacc tcgcgtacgg aaggctcgag gacggcgggc tgcgctgctc gttccacggc
     3841 tggctgtacg acgtcgcggg caaatgcctg gagacgccag ccgagcccga gggcagccgc
     3901 ctctgcgaac acatccgcct gcgctcctgc ccggtggtcg agaagagcgg cgtgctcttc
     3961 gcctacctcg gcgccggcga gccacctgcg ttcccgcagt tcgattgctt cgtcgcaccc
     4021 gacgcgtaca cgttcgcgtt caagggctat tgggactgta actggctcca ggcgctggaa
     4081 gtgggcatcg atccgtcgca cgcgtcgtgg ctacacaagt tcttcgagga cgaggacccg
     4141 gcggcgagct acgggcgcca gttccgcggc acgccggccg actcgcggat gccgatctcg
     4201 aaagtgctgc gcgagtacga
//
LOCUS       DASTPD010000031         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1441018, whole genome shotgun sequence.
ACCESSION   DASTPD010000031 DASTPD010000000
VERSION     DASTPD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441018"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFR57_01435"
     CDS             complement(<1..773)
                     /locus_tag="VFR57_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEU0258067.1"
                     /translation="MAPLVLDSPHSGHDFPADFGAIVTEAELRESEDCYVDELYAAAH
                     ELGVPLLAATFPRTYLDPNRNAGDIDLDLVEGRLPADWPWEYRPSGKARIGKSLIWRT
                     LEDGRPIYGRKLAPETVRRRIDRVHAPYHRSLQELLEKAVWKHGRVYHINCHSMRSVA
                     GKQSEDGAGSVRPDFVIGDRDGTTCEARFTELVRETLAGMGYRVTVNDPYKGVELIRA
                     YSNPKAGRHSLQIEINKRLYMDERTLQKNAGFCALQKNLG"
     gene            791..1456
                     /locus_tag="VFR57_01440"
     CDS             791..1456
                     /locus_tag="VFR57_01440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258068.1"
                     /translation="MESYYARRAAEYEKIYDKPERQADLARLRNDIPALFKGERVLEI
                     ACGTGYWTPLIAQQARSVLAVDYNEETLAIARSKKYPEGNVRFERADAYALPDWQEQF
                     SACYAGFWWSHVPLARLDAFLNGLQARLEPGARVVFMDNRYAEGSSTAISRKDPQGNT
                     YQRRRLADGSSHEVLKNFPTAAELRARLGRFGTELTFSEYEYYWLATYRIMAGTNPGG
                     GKR"
     gene            1453..>2364
                     /locus_tag="VFR57_01445"
     CDS             1453..>2364
                     /locus_tag="VFR57_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258069.1"
                     /translation="MIRFLLAVLFFLATAATAQELRIGLASEPTAMDPHYHNLSPNNS
                     LLSHIFESLVGQDERQRLTPDLAESWKPIDPTTWEFKLRKNVRFHDGTPFTADDVIFS
                     FERAPNVEGSPSSFGIYARGKTFVKVDDHTLHVKTAAPYPLMPNDVSQVFIVSRKHGQ
                     GAKTPDYNSGKAAIGTGPYRFVEYTPGNRIVMQRNEQYWGDKPVWQRVIFRGIKSDPS
                     RVAALLAGDVDMIDEVPAVDMERLKKDPKLTIAQTVSNRIIYLHLDHFRDDSPFVRTK
                     DGGAIKNPLRDVRVRKAISMAIDRDVIV"
BASE COUNT          429 a          769 c          757 g          409 t
ORIGIN      
        1 ccgaggttct tctggagggc gcagaacccg gcgtttttct gcagcgtgcg ctcgtccatg
       61 tagagccgct tgttgatctc gatctgcagg ctatggcgtc cggccttggg attcgagtac
      121 gcgcggatca gctcgacgcc cttgtaggga tcgttcaccg tcacgcgata gcccatgccg
      181 gcgagcgttt cgcgcacgag ctcggtgaag cgcgcctcgc aggtcgtgcc gtcgcgatcg
      241 ccgatgacga aatccgggcg cacgctgccc gcgccgtcct cgctctgctt gccggccacc
      301 gagcgcatgg agtggcaatt gatatggtag acccgcccgt gtttccagac ggctttttcg
      361 agcagctcct gaagcgagcg gtggtacgga gcgtgcacgc gatcgatgcg gcggcgcacg
      421 gtttccggcg cgagcttgcg cccatagatc ggccggccgt cttccaacgt gcgccagatg
      481 agcgatttgc cgatacgcgc cttgccgctc ggccggtact cccacggcca atcggcagga
      541 aggcgtcctt cgacgaggtc gaggtcgatg tcgccggcat tgcggttggg gtcgaggtac
      601 gtgcgcggaa acgtcgcggc gaggagcggc acgcccagct cgtgcgccgc ggcgtagagc
      661 tcgtcgacgt agcaatcctc ggactcgcgc agttcggcct cggtgacgat ggcgccgaag
      721 tcggccggaa aatcgtgacc ggaatgcggg gaatcgagta ccagcggcgc catcgctgta
      781 gcatacttga atggaaagct attacgcccg gcgcgccgcc gagtacgaaa agatctatga
      841 caagccggag cgccaggccg atctcgcgcg gctgcgcaac gacatcccgg cgcttttcaa
      901 gggcgagcgg gtgctcgaga tcgcgtgcgg caccggctac tggactccgc tcatcgcaca
      961 gcaggcgaga tcggtcctcg ccgtcgacta taacgaggag acgctggcga tcgcgcgaag
     1021 caagaagtat ccggagggca atgtgcgctt cgagcgtgct gacgcctatg ccctgccgga
     1081 ttggcaggaa cagttctccg cctgctacgc cggcttttgg tggtcgcatg tcccgctcgc
     1141 caggctggac gcgtttctaa atgggctgca agccaggctc gagccgggcg cgagggtagt
     1201 gttcatggac aaccgctatg ccgagggatc gagcaccgcc atttcccgca aggacccgca
     1261 ggggaacacc taccagcgcc gccgcctcgc cgacggctcg agccacgagg tgctgaaaaa
     1321 tttcccgacg gcagcggagc tgcgcgcgcg cctgggccgg ttcgggaccg agctgacgtt
     1381 ttcggagtac gagtactact ggctcgccac ctatcgcata atggccggga caaacccggg
     1441 aggagggaaa cgatgattcg gttcttgctc gcagttcttt tctttcttgc gaccgcggcc
     1501 accgcccagg agctgcgcat cggcctcgcg tccgagccga cggcgatgga tccgcactac
     1561 cacaacctgt cgccgaacaa ctcgctcctc agccacatct tcgagagcct ggtgggtcag
     1621 gacgagcgcc agcgcctcac cccggacctc gccgagtcct ggaaacccat cgacccgacg
     1681 acctgggaat tcaagctgcg caagaacgtg cgcttccatg acggcacccc gttcaccgcc
     1741 gacgacgtca tcttcagctt cgagcgcgcg ccgaacgtcg agggcagccc gtcgagtttc
     1801 ggcatctacg cgcgcggcaa gaccttcgtc aaggtcgatg accacacgct gcacgtgaag
     1861 accgctgctc cctacccgct catgccgaac gacgtgtctc aggtcttcat cgtctcacgc
     1921 aagcacggcc agggcgcgaa gacgcccgac tacaactcgg gcaaggccgc gatcggcacc
     1981 ggcccgtatc gcttcgtcga gtacacgccg ggcaatcgca tcgtgatgca gagaaacgag
     2041 cagtactggg gcgacaagcc cgtttggcag cgcgtgattt tccgcggcat caagtccgat
     2101 ccctcgcgcg tcgcggcgct gctcgcgggc gacgtcgaca tgattgacga ggtgccggca
     2161 gtggacatgg agcggctcaa gaaagacccg aagctcacga tcgcgcagac cgtctcgaac
     2221 cgcatcatct acctgcacct cgatcatttc cgcgacgatt cgcctttcgt gcggacgaag
     2281 gacggcggcg cgattaagaa ccccctgcgc gacgtgcgcg tgcgcaaggc gatttccatg
     2341 gcgatcgacc gcgacgtcat cgtg
//
LOCUS       DASTPD010000032         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1441853, whole genome shotgun sequence.
ACCESSION   DASTPD010000032 DASTPD010000000
VERSION     DASTPD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441853"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFR57_01450"
     CDS             <1..655
                     /locus_tag="VFR57_01450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258070.1"
                     /translation="VWDLGAGDGKIAIMAAKKFGARAVGIEYNADMVKHANENAQKAG
                     VAGTGAGKAVIRHGDIFATDFTQASVITLYLLPALNMKLRPQLLSMRPGTRVVSHSFS
                     MEDWEADEISTLDGRRAYFWIVPANVMGNWTLDGGGQHHDMTLEQTFQKVSGTLALGP
                     IHAGLRDVKLRGTAISFAYVDQNKVRRDFTGRVNGSKMEGSFRDEKGAEGKWTATKK"
     gene            complement(699..938)
                     /locus_tag="VFR57_01455"
     CDS             complement(699..938)
                     /locus_tag="VFR57_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU0258071.1"
                     /translation="MAEPLIAQKSPYVVELAPGDYWWCRCGHSKRQPFCDGSHKTTEF
                     TPVKFSITEAQKIWLCGCKRSGNKPFCDGSHKKLT"
     gene            complement(923..2347)
                     /gene="dnaB"
                     /locus_tag="VFR57_01460"
     CDS             complement(923..2347)
                     /gene="dnaB"
                     /locus_tag="VFR57_01460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0258072.1"
                     /translation="MPVAQPLARPGVGDPQLVALKLPPHSVEAEQSLLGALLLDNQAF
                     DRVADLVSAGDFYRDDHRRIWRHIARLVEASRPADVVTVSESIEASEDKDKTGGAAYL
                     AALAQNTPSALNIRRYAELVRERAVQRRLAQVATEIAEAALAPTGKDVGQLLDEAESR
                     IFQIAESGARKDQGLLGISPVLAKVFERIDHLHSQDNPSDVTGVPSGFIDLDRKTAGL
                     QPGDLIIVAGRPSMGKTALALNIAEHVALHPSVGLPVAIFSMEMSATQLAMRMLSSMA
                     AVDQTKLRTGRLDNREWADLTDAIGKLNEARIHVDETAALNALELRARARRMKREYGK
                     LGLIVVDYLQLMSASSQGENRATEISEISRSLKALAKELDVPVIALSQLSRAVEQRND
                     RRPLMSDLRESGAIEQDADVILFIYREEVYAPEKEEAKGRAEVIIGKQRNGPIGTIAL
                     TFRGQFTRFANALAFDGGGQWPNR"
     gene            complement(2650..3099)
                     /gene="rplI"
                     /locus_tag="VFR57_01465"
     CDS             complement(2650..3099)
                     /gene="rplI"
                     /locus_tag="VFR57_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0258073.1"
                     /translation="MEVILLEKIANLGELGQVVKVKDGYARNYLIPHGKAKRATAENR
                     KAFEARRAELEQAQAIALAKAQERSGRLDGLTLQITQKAGPDGRLFGSVTNYDIVEAL
                     KKQGHEVERAQIRMPQGPLKQVGDFPIEVALHTDVAVTITVSVLGEQ"
     gene            complement(3119..3412)
                     /gene="rpsR"
                     /locus_tag="VFR57_01470"
     CDS             complement(3119..3412)
                     /gene="rpsR"
                     /locus_tag="VFR57_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0258074.1"
                     /translation="MPPPRRGKGKGKGKFGKDKKDRGQRALFRRRKFCRFTAEKVEWI
                     DYKDVETLKDFVNETGKIIPARITGTAAGYQRQLSEAIKRARFLALLPYTDLH"
     gene            complement(3390..3668)
                     /gene="priB"
                     /locus_tag="VFR57_01475"
     CDS             complement(3390..3668)
                     /gene="priB"
                     /locus_tag="VFR57_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEU0258075.1"
                     /translation="MRYTPAGVAAVEFRLTHRSEQVEAGGRRTVEAEVGAVAFDTQAK
                     LLAGRPLGSAVKVEGFLGAKSKRSKRLVLHVTNIEFIEGEQHATAAQR"
     gene            complement(3679..4083)
                     /gene="rpsF"
                     /locus_tag="VFR57_01480"
     CDS             complement(3679..4083)
                     /gene="rpsF"
                     /locus_tag="VFR57_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0258076.1"
                     /translation="MRHYEICFIVHPDQSEQVPQMVERYKGLIAQRNGTVHRLEDWGR
                     RQLAYPIAKVHKAHYVLMNIECDNETLKELEHSFKFSDAVLRHLIVAMPKAHTNPSPM
                     MREEKSKSMMERGAGASGEVAPEPGRAERPAA"
BASE COUNT          665 a         1332 c         1317 g          770 t
ORIGIN      
        1 cgtctgggac ctgggggcgg gcgacggcaa gatcgcgatc atggcggcga agaagttcgg
       61 cgcccgcgcc gtcggcatcg agtacaacgc ggacatggtg aagcacgcga acgaaaacgc
      121 gcagaaggcg ggcgtcgcgg ggaccggcgc gggcaaggcg gtgatccggc acggcgacat
      181 cttcgccacc gacttcaccc aggccagcgt catcacgctg taccttctac ccgctctcaa
      241 catgaagctt aggccgcagc tcctgtcgat gcggcccggc acgcgcgtgg tgtcgcactc
      301 gttcagcatg gaggactggg aagccgacga gatctccacg ctcgacggcc ggcgagccta
      361 cttctggatc gtgcctgcca acgtgatggg gaactggacc ctcgacgggg gcggccagca
      421 ccacgacatg acgctcgagc agaccttcca gaaggtgagc ggcaccctcg cgctcggccc
      481 gatccacgcc gggctgcgcg acgtaaagct ccgcggcacc gcgatcagct tcgcctacgt
      541 cgaccagaac aaggtgcgcc gcgacttcac cggccgcgtc aacggcagca agatggaagg
      601 cagcttccgc gacgagaagg gcgcggaggg caagtggacc gctaccaaga aataggcata
      661 cctggaaagc ccggataccg caggtatccg gcagggttct aagtcagctt cttgtgtgag
      721 ccgtcgcaga agggcttgtt gccgctgcgc ttgcaaccgc acagccagat tttctgcgcc
      781 tcggtgatgc tgaacttcac cggcgtgaac tccgtcgttt tgtgcgagcc gtcgcagaac
      841 ggctggcgct tggagtggcc gcagcgacac caccagtagt cgccgggcgc gagttcgacg
      901 acatacgggg atttctgcgc gatcagcggt tcggccattg cccgcctccg tcaaaggcaa
      961 gggcgttcgc gaagcgggtg aactgccctc tgaaagtaag cgcaatggta ccgatcggcc
     1021 cgtttctttg ctttccgatg atcacttccg ccctgccctt cgcttcctct ttctccgggg
     1081 catagacctc ttcgcggtag ataaagagga tcacgtcggc gtcctgctcg atcgcgcccg
     1141 actcgcgcag gtccgacatc agcggccggc ggtcgttcct ctgctcgacc gcccgcgaca
     1201 gctgcgagag cgcaatcacc ggcacatcga gctccttggc gagcgccttg agcgagcggg
     1261 agatctccga gatctcggtc gcgcggttct cgccctggga gctggccgac atcaactgca
     1321 ggtagtcgac gacgatcagg ccgagcttgc cgtactcgcg cttcatgcgt cgcgccctgg
     1381 cgcgcaattc gagagcattg agcgccgcgg tctcgtcgac gtggatgcga gcctcgttca
     1441 gcttgccaat cgcgtcggtg aggtctgccc attccctgtt gtcgagcctt ccggtgcgca
     1501 gcttcgtctg gtcgaccgcg gccatcgatg agagcatgcg catggcgagc tgggtcgcgc
     1561 tcatttccat gctaaagatc gccaccggca agccgacgct gggatgcaag gcgacgtgct
     1621 cggcgatatt gagggcgagg gcggtctttc ccatcgacgg ccgcccggcc acgatgatca
     1681 ggtccccggg ctgcaatccc gcggtcttgc ggtcgaggtc gatgaagccc gaaggaactc
     1741 cggtgacgtc ggagggattg tcctgactgt gaaggtggtc gatgcgttcg aacaccttgg
     1801 cgagaacggg gctgatgccc aggaggcctt ggtccttgcg cgcgcccgac tccgcgatct
     1861 ggaagatcct cgattccgcc tcgtcgagga gctgccctac gtctttgccg gtcggcgcga
     1921 gcgcggcctc ggcgatctcc gtagcgacct gcgcgagacg ccgctgcacc gcgcgctcgc
     1981 gcacaagctc ggcgtagcgc cggatgttaa gcgcgctcgg cgtgttctgc gcgagcgccg
     2041 cgaggtaggc cgcgccgccg gtcttgtcct tgtcctcgct cgcctcgatc gactcggaca
     2101 ccgtgaccac gtcggccggc cggctcgcct ccaccaggcg cgcgatgtgg cgccagatgc
     2161 ggcggtggtc gtcgcggtag aaatcgcccg ccgagacgag gtcggcgacg cggtcgaacg
     2221 cctgattgtc gaggagcagc gcgccgagca gcgactgctc ggcctcgacg gagtgcggcg
     2281 gcagcttgag cgcgacgagc tgcggatcgc cgacgcccgg gcgcgctagt ggctgtgcaa
     2341 ctggcatttt gttctgacct ccccggcgcg catcatagga agtgaggccg ggtttccgat
     2401 ttgacaagtc gaaacgcgcg ctttaccaat gctctcgcgc cgcggcaccg ctgaaaattt
     2461 gaccatccga ggctaacgga tcctgaccgc tgtggatagg ctgtgagcag gctgtcggta
     2521 gtttgtggat ggcgctgtac aactccaaat taaatctctt gacacgccgg ccactctatt
     2581 actgtataaa cgtccacgca actgaaatga atcaggggcc ggtaaggccc gtctcgccgc
     2641 gctgagagct tattgctcgc cgagcaccga gacagtgatg gtgacggcga cgtcggtgtg
     2701 cagcgcgacc tcaatcggaa agtcgcccac ttgcttgagc ggtccctgcg gcatgcggat
     2761 ctgcgcgcgc tcgacctcgt ggccctgctt cttgagcgct tccacgatgt cgtagttggt
     2821 gaccgagccg aagagacggc cgtcgggccc ggccttttgc gtgatctgca gcgtcaggcc
     2881 gtcgagcctg cctgagcgct cctgtgcctt ggcgagagca atggcctgcg cctgctcgag
     2941 ctcggcgcgg cgcgcttcga acgccttgcg gttctccgcg gtcgcgcgct tcgccttgcc
     3001 gtgcgggatg agatagttgc gcgcgtaccc gtccttcacc ttcaccacct ggccgagctc
     3061 gcccaggttc gcgatcttct cgagaagtat cacttccata gcagtatcga cgtcgatctc
     3121 aatgcaggtc cgtgtagggc agcagcgcaa gaaagcgcgc acgcttgatc gcctccgaca
     3181 gctggcgctg gtagcccgcg gcggtcccgg tgatgcgcgc cgggatgatc ttgcccgtct
     3241 cgttgacaaa atccttgagc gtctcgacgt ccttgtagtc gatccactcc accttctcgg
     3301 ccgtgaagcg gcagaacttg cgccggcgaa agagggcccg ctggccgcgg tccttcttgt
     3361 ccttgccgaa cttcccttta cccttgcctt tacctctgcg cggcggtggc atgttgctct
     3421 ccttcgatga attcgatgtt cgtcacgtgg agcacgagcc gcttggagcg cttgctcttt
     3481 gctcccagaa atccctcaac tttcaccgcg ctgccgagcg gcctgccggc gagcagcttc
     3541 gcctgcgtgt cgaaggccac cgcgcccacc tcggcctcga ccgtcctgcg cccgcccgcc
     3601 tcgacctgct cggagcggtg cgtgagcctg aactcgacgg cggcgacgcc ggccggcgtg
     3661 taacgcaacg cccccagctc aagcagctgg ccgctcagct cgacccggtt caggcgccac
     3721 ctcgccggag gcgccagcgc cgcgctccat catcgacttc gacttctcct cgcgcatcat
     3781 gggggagggg ttggtgtgcg ccttgggcat ggctacgatc agatggcgca gcactgcgtc
     3841 gctgaacttg aacgagtgct cgagctcctt cagcgtctcg ttgtcgcact cgatgttcat
     3901 gagtacgtag tgcgccttgt gaaccttggc gatcgggtag gcgagctgcc ggcggcccca
     3961 gtcctcgagg cggtgcacag tgccgttgcg ctgggcgatc aggcccttgt agcgctcgac
     4021 catctgcggc acctgctcgc tctggtcggg atggacgatg aaacagatct cgtaatgccg
     4081 catg
//
LOCUS       DASTPD010000033         7913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_625997, whole genome shotgun sequence.
ACCESSION   DASTPD010000033 DASTPD010000000
VERSION     DASTPD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625997"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1013
                     /locus_tag="VFR57_01485"
     CDS             30..1013
                     /locus_tag="VFR57_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258077.1"
                     /translation="MLAKRGLAAVIIGVLLIAPSLVAAQPSQTLPQVAMLLTGSPSDG
                     DRMTSAFRGGLRDLGYVEGRNIVVLARWAETPQRLSELAAEVVRNNVDVIVTQGTPAA
                     HAAKRATNTIPIVMATSGDPVALGLVASLARPGGNVTGNTILAPDLNGKRLELIRDLV
                     PGVSRIAVLANPASPATAVDIKAAQAAARGLGLLLQVLEVREQNDFDRAFTAAGESGA
                     TALLVQADPFILANRARIAKLAVASRLPAVGGISGFAEAGGLADYGPDITAMFRRAAT
                     YIDKILKGAKPADLPVEQPTRLELVINTRTAKALNLQIPPSILLRADRIIN"
     gene            1069..1497
                     /locus_tag="VFR57_01490"
     CDS             1069..1497
                     /locus_tag="VFR57_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0258078.1"
                     /translation="MTDLKLPPLASRFIDVESLPWEKTRYPGVEQKTLLVEKESGLLT
                     ALMRMAPGAKLPDHEHVKIEQTYVLEGELHCPEGICRPGQFVWRPAGSRHHAWAGPKG
                     GLFLAMFQAPNKFFQSDGKVTDFLGRDWEATWGKDHNPVP"
     gene            complement(1642..2652)
                     /locus_tag="VFR57_01495"
     CDS             complement(1642..2652)
                     /locus_tag="VFR57_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU0258079.1"
                     /translation="MNRILTTVVGSYPLPDWLARAPSQQALVDATRVVIATQEQAGID
                     LVADGELYRFDVNHPETNGMIEYFVRPMAGVRSDIGFSEWLDYSRGAGMRFRAKPPGV
                     VEGEIGAGTLNLKLPCARAKALATKPLKFTLTGPHMLAKTLHDRHYGSAEKLAHAIAD
                     VLAEQVKHLDADVVQLDEANLPGHPDEWKWAAAAINKVLSKIPRQAKGAVHLCFGNYG
                     GQSIQKGTWAKLLDYLNALDVDHIVMETAHRPPDELAVFKDLRKDIGFGLGVVDIKRT
                     EIEKPEDVARAIERAEKTLGKDRVKYIHPDCGFWMLPRNVADGKIRALPAGRDLYEGR
                     KS"
     gene            2676..3689
                     /locus_tag="VFR57_01500"
     CDS             2676..3689
                     /locus_tag="VFR57_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258080.1"
                     /translation="MRVFAGVEDLEEFMTRPTRELEADLARADGDLIILGVGGKMGPT
                     LARMAKRAAPAKRVIGVARFSEKGLKEKLQSQGIECLACDLLDRATLERLPRAKNVVF
                     MAGHKFGAAGDPSFTWAMNVAVPFMVAEAFRESRIVVFSTACVYPYVSVHGAGAPEDM
                     PTTPPPGDYASSCVGREQMFLHASRRYGTPGRLVRLSYAIDMRYGVLHDVASAVFDAK
                     PVALGMGYANVIWQGDANEQSLRLLAHCTSPATPLNVSGPKTSVRLLAEEFGKRFSRR
                     PNFSGTEATTAWLVDTAESKKLLGAPRVPLETMIDWVADWVGRGQPSLGKPTHFETRD
                     GKY"
     gene            3673..4497
                     /locus_tag="VFR57_01505"
     CDS             3673..4497
                     /locus_tag="VFR57_01505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0258081.1"
                     /translation="MTGSIEHPLGEQHLAGCLALSRAANWNQNEADWRLMLATGHGWG
                     ITLLDGTLAASTLVLPYQTFAWISMVLVLPEHRRKGYATHLLRVALADLRNRALTPIL
                     DATPAGREVYRQEGFRDTWTFKRLQLEKGVRTLFPEKGPDSALAWEKVLRLDREAFGA
                     SREPLLRNLATRLPRAAFFTEDGFVLGREGREALQVGPLVARHEDAAIGLLSQALRAI
                     APPLYVDAVDHAPRLQQWLTKQGFVYQRPFTRMVHGAGRAPGNEKLVYLVAGPELG"
     gene            4507..6063
                     /locus_tag="VFR57_01510"
     CDS             4507..6063
                     /locus_tag="VFR57_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0258082.1"
                     /translation="MSYDFIVIGAGHNGLACAAYLARAGAKVLVLERSARVGGGCHTA
                     EATLPGFKHNLCSVVHTHIPLSPVYRDLELERHGVRYVYPDHLRGTIFPGHKSLVMHR
                     DADKMAAELGKFSARDARTFRQLVADYGEFIDSTYLPLMYSPPLPPSLQSAQLEQSEE
                     GRQLLQWQAATPVQLLSELFESEEVKVHFLARMTVLGFAPDQFGQGWICLFRILKAEA
                     PICVGGSQQLAHGLRSALEAGGGVVRTDSAVKRMLLRGNKAVGVELMDGERIAAAKAL
                     VTNVEPKKSFLSMVGEEFLPQSFATRVRNYHSRGRSLFVLHLALSEPPQYRAGAHNPP
                     VNVAFSQEMFGGSVHDQVRAYQEIALGSAPRKEMLQITLPTLFDPSQAPAGKHTAVVW
                     QYAPYALEGGWPKLRDEYARHVLGLWQSYAPNMTRDKVLAMKIQDPTDTERLNDNLVN
                     GVDVVGDMTPDQMGYFRPFAGWSSYRTPIEGLYMAGGYCHPGGGVHAGAGHNAAGVIV
                     EDFKLKKWWG"
     gene            6051..6572
                     /locus_tag="VFR57_01515"
     CDS             6051..6572
                     /locus_tag="VFR57_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0258083.1"
                     /translation="MVGLMPEREARFNVNAPPAELWKFLRDFRALCTCIPGVERIHVV
                     DQRTAELTVKEKVGVVPLTVNLRAEIDSEEPPRRLHATARAEHLTMAIEVTLRATGSG
                     TELVSHFEVKGEGPLKPVVDRLFDKRAAERTAQFTECIGKRFGAVTAPREPPLGWLAR
                     LWRLVRGLSRPSS"
     gene            complement(6544..7542)
                     /locus_tag="VFR57_01520"
     CDS             complement(6544..7542)
                     /locus_tag="VFR57_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258084.1"
                     /translation="MRKLIIAAAAASLPFAATPAAAQKIDFILNWVPGGDHAPYYYAK
                     KLGWYQKEGIDLNLEPGKGSALATQKVGAGANPVGLADMGGVLVAKGKGADTVAVFNV
                     YANSPQGLYWLKSSGIKGIKDFPGKKIGNPAADGARTMWPALAKANGIDPKSVTWVNI
                     DANAKLSALKAKSIDITTSFYNLHHVFQRELGADMGFLAWRDIGLNPYGNSVIVNAEY
                     LKTNQPLVAKFVRVTQRAFAECVKEPKPCVQALIDANGALSFDNELVNWQLVEVLMSD
                     KISQTVALGWHDDARMQADYALVRDYVGVDKPFDVKSTYTNEFLDKSIRMKAVKAP"
     gene            7602..>7913
                     /locus_tag="VFR57_01525"
     CDS             7602..>7913
                     /locus_tag="VFR57_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0258085.1"
                     /translation="MTLPSEIAALLRRGTVIPAHPLALDARRQRALTRYYLDAGSGGL
                     AVGVHATQFAIREAGLYEPVLRIALEETKTWAKRPVVLIAGLAGKTSAAKREAQLARS
                     LG"
BASE COUNT         1330 a         2610 c         2592 g         1381 t
ORIGIN      
        1 gagcacttga agccgtgaac gtgcgttgaa tgcttgcgaa gcgcggcctc gctgcggtca
       61 tcatcggggt gctgctcatt gcgccgtcgc ttgttgccgc gcagccgtcg caaacgctgc
      121 cgcaggtggc gatgctgctc accgggtcgc catcggacgg cgaccgaatg acctcggctt
      181 ttcgcggcgg gcttcgcgac ctcggttatg ttgagggacg taacattgtg gtgctggctc
      241 gctgggcgga gaccccacag cgcctgtccg agctggccgc ggaagtcgtc cggaacaatg
      301 tcgacgtgat tgttacgcag ggaacgccgg cggcccacgc cgcgaagcgc gcgacaaaca
      361 cgattccgat tgtcatggct acgagcgggg atccggtagc cttagggctg gtcgccagtc
      421 tggcccgccc tggaggcaac gtgaccggca acaccatcct cgcgccagat ctcaacggaa
      481 agcggctcga gttgatcagg gacctggttc ccggcgtctc gcgcatcgcc gtcctcgcga
      541 atccggcgag cccggccacg gctgtggaca tcaaggccgc gcaagcggcg gccaggggtc
      601 tcggcctgtt gcttcaagtg ttggaggtgc gcgaacagaa cgattttgac cgggcgttca
      661 cagccgcagg tgaatcgggc gccacagcac tgctggtgca ggccgatccg ttcatcctgg
      721 ccaatcgggc gcgcatcgcg aagctggcgg tcgcaagtcg tctgccggcg gtagggggaa
      781 tcagcgggtt cgcggaagcc ggcggcttgg ccgattacgg accggatatc acggcgatgt
      841 tccggcgggc agccacgtat atcgacaaga tcctgaaggg cgccaagcca gccgatctgc
      901 cggtggaaca gccgacccgg ctcgagttgg taatcaacac ccggaccgct aaagcgctca
      961 atctgcaaat tcccccgtcg attttgctgc gggctgatcg catcatcaat tgacgagtga
     1021 ccggaaagcg tggaataccc tatcgaccga gtgactgagg acttgccgat gaccgacctg
     1081 aaactgccgc cgcttgcctc gcgctttatc gacgtggaat ccctgccctg ggaaaaaacc
     1141 cgctatcccg gggtcgagca gaagaccctg ctggtcgaga aggagtccgg gctccttacg
     1201 gcgctcatgc gcatggcgcc gggcgcgaag ctgcccgacc atgagcacgt caagatcgag
     1261 cagacctacg tgctggaagg cgagctgcac tgccccgaag gaatttgcag gccaggccag
     1321 ttcgtctggc gtcccgccgg cagccgccac cacgcgtggg ccggccccaa gggtgggctc
     1381 ttcctcgcca tgttccaggc accgaacaag ttcttccagt ccgacggcaa ggtcaccgac
     1441 ttcctgggcc gcgactggga agccacctgg ggcaaggatc acaacccggt tccatgattc
     1501 tcaaatgaac aggcggcagc cgctgggcgc cctgttcgcc tttggggcga tgccgcgagc
     1561 actgcgtgca cagacagtgc caaaagagca aggctttgcc atcccgcacg cgcttctgca
     1621 gcgggcggac cgagttattc actaagactt ccggccctca tacaggtcgc gtcccgcggg
     1681 cagcgcgcga attttcccgt cggcgacgtt gcgcggcagc atccagaagc cgcaatcggg
     1741 gtggatgtac ttcacgcggt ccttaccgag cgtcttttcg gcgcgctcga tggcgcgcgc
     1801 gacgtcttcg ggcttctcga tctcggtgcg cttgatgtcg accacgccca ggccgaagcc
     1861 gatgtccttg cgcagatcct tgaacaccgc gagctcatcc ggcgggcggt gcgccgtttc
     1921 catcacgatg tggtcgacgt cgagcgcgtt caggtagtcg aggagcttcg cccacgtgcc
     1981 cttctggatc gattggccgc cgtagtttcc gaaacacagg tgcaccgcgc ctttcgcttg
     2041 ccgaggtatt ttcgacagaa ccttgttgat ggcggccgcg gcccatttcc actcgtcggg
     2101 atggccgggc agattggcct cgtcgagctg gacaacatcg gcatcgaggt gcttcacctg
     2161 ttcggcgagc acatcggcga tcgcgtgcgc gagcttctcg gcgctgccgt aatgccggtc
     2221 gtgcagcgtc tttgccagca tgtgcggtcc ggtgagcgta aacttgagcg gctttgttgc
     2281 gagcgccttc gcgcgcgcgc acggcagctt gaggttcagc gtgcctgcgc cgatctcgcc
     2341 ctcgaccacc ccgggcggct tcgcgcggaa gcgcatgccc gccccgcgcg agtagtcgag
     2401 ccactcggaa aagccgatgt ccgagcgtac gccggccatc ggccgcacga agtactcgat
     2461 catgccgttg gtttcgggat gattgacgtc gaagcggtag agctcgccgt cggcgaccag
     2521 gtcgattccc gcctgctcct gcgtcgcgat cacgacgcgc gtcgcgtcga cgagcgcctg
     2581 ctgcgacggc gcgcgcgcga gccagtccgg aagcggatac gagcccacga cggtggtcag
     2641 aatacggttc attcgtggat tatagaattg ggcgaatgcg cgtcttcgca ggcgtggagg
     2701 acctcgagga gttcatgacg cggcccacgc gcgagctcga ggcggatctc gcgcgcgccg
     2761 acggcgacct gatcatcctg ggcgtgggcg gcaagatggg cccgacgctc gcgcgcatgg
     2821 cgaagcgcgc cgcgccggcg aaacgcgtca tcggtgtcgc gcgcttctcc gaaaaaggtc
     2881 tcaaggagaa gctgcaaagc cagggcatcg agtgcctggc ctgcgatctt ctcgatcgcg
     2941 cgacgctcga gcgtctcccg cgcgcgaaga acgtggtgtt catggccggc cacaagttcg
     3001 gcgccgccgg cgacccttct ttcacctggg ccatgaatgt cgccgtgccg ttcatggtgg
     3061 cggaggcgtt ccgcgagtca cgcatcgtcg tcttctcgac cgcctgcgtg tacccgtatg
     3121 taagcgtgca cggcgccggc gccccggaag acatgccgac cacgccgccg ccgggcgatt
     3181 atgcaagctc gtgcgttggg cgcgagcaga tgttcctgca cgcttcgcgc cgctacggca
     3241 cgcccgggcg cctggtacgg ctctcctacg ccatcgatat gcgctacggg gtgttgcatg
     3301 acgtcgcttc cgccgttttc gacgcgaaac ccgtggcgct cggcatgggc tatgccaacg
     3361 tgatctggca gggcgacgcg aacgagcaat cgctgcgcct gctcgcgcac tgcacctctc
     3421 ctgcaactcc gctcaacgtg agcggcccga agacaagcgt gcgtttgctc gcggaagagt
     3481 tcggcaagcg cttctccagg cgaccgaatt tctcgggcac cgaagccacc accgcctggc
     3541 tggtggatac ggccgagtcg aagaaattgc taggggcgcc tcgcgtgccg ctcgaaacga
     3601 tgatcgactg ggttgccgac tgggtcgggc gcggtcagcc gagcttgggc aagccgacgc
     3661 acttcgagac gcgtgacggg aagtattgag cacccgctcg gcgagcaaca cctcgccggc
     3721 tgcctcgcgc tctccagggc ggcgaactgg aaccagaacg aggctgactg gcgcctcatg
     3781 ctcgcgaccg gccacggctg gggtatcacg ctcctcgacg gcacgctcgc cgcctccacg
     3841 cttgttcttc cgtaccagac cttcgcctgg atcagcatgg tgctcgtgct gcccgagcac
     3901 cgaaggaagg gctacgccac gcatctcctg cgcgtagcgc tcgcggacct gcgtaatcgg
     3961 gctctgaccc cgattctcga cgccacgccg gcaggccgcg aggtgtaccg ccaggaaggc
     4021 ttccgcgaca cctggacctt caagcgcctc cagctcgaaa aaggggtcag gacccttttt
     4081 ccggaaaaag ggccggactc cgccctggcc tgggagaagg tccttcggct cgaccgcgaa
     4141 gccttcggcg cgagccgcga gccgctgctg cgcaacctcg cgactcgatt gccgcgcgcg
     4201 gcctttttca ccgaagatgg cttcgtcctc ggccgggaag gaagagaagc cctgcaagtc
     4261 ggacctctcg tcgcccgtca cgaggacgct gccatcggcc tgctttcgca ggcgttgcgc
     4321 gcgatcgcgc cgccgctcta cgtagatgcg gtggaccacg cgccgcggct gcagcagtgg
     4381 ctgacgaagc agggcttcgt ctaccagcga ccgtttaccc gcatggtgca tggcgcaggg
     4441 cgcgcgccgg gaaacgagaa gctcgtctat cttgtggccg ggcccgagct cggctaggag
     4501 ggtcccttgt cctacgactt tatcgtcatc ggcgccgggc acaacggcct cgcctgtgcc
     4561 gcctatctcg caagagcggg cgctaaggtg ctggtgctgg agcgaagcgc ccgcgtaggc
     4621 ggcggctgcc acaccgcgga agcgacgctg ccgggcttca agcacaacct ctgctcggtg
     4681 gtgcacaccc acattccttt gagcccggtg taccgcgacc tcgagctcga gcgtcacggc
     4741 gtgcgctacg tctatcccga tcacctgcgc ggcacgatct tccccggcca caagagcctc
     4801 gtcatgcacc gggatgcgga caagatggcg gccgagctcg gcaaattttc cgcgcgtgat
     4861 gcacgcacct tcaggcagct cgtcgccgac tacggcgaat tcatcgactc gacgtacctt
     4921 ccgctcatgt actcgccgcc gctgccgccg tcgctgcaga gcgcccagct cgagcaatcc
     4981 gaggaagggc ggcagctctt gcagtggcag gcggccacgc cggtgcagct cctaagcgag
     5041 ctcttcgaga gcgaggaagt gaaggtgcat ttcctcgcgc gcatgacggt gctcgggttc
     5101 gcgcccgacc agttcgggca gggttggatc tgcctctttc gcatcctcaa ggcggaggca
     5161 cccatctgcg tcggcggctc gcagcagctt gcgcacggtc ttagaagcgc gctcgaggcc
     5221 ggcggcggcg tggtgcgcac cgacagcgcg gtgaaacgaa tgctcctgcg tggcaacaag
     5281 gctgtcggcg tcgagctcat ggacggcgag cgcatcgcgg ccgcgaaggc gctcgtcacc
     5341 aacgtcgagc caaagaagag cttcctctcc atggtcggcg aagaattcct gccgcagtcc
     5401 ttcgccaccc gggtcaggaa ctaccattcc cgcgggcgct cgcttttcgt gctgcacctc
     5461 gcgctctccg agccgccgca gtaccgggcc ggcgcgcaca acccgccggt gaacgtggcc
     5521 ttcagccagg agatgttcgg cggcagcgtg cacgaccagg tccgcgccta ccaggagatc
     5581 gcgcttggaa gcgcgccgcg caaggagatg ctgcagatca cgctgccgac gctgttcgac
     5641 ccttcgcagg ctcccgcggg aaagcacacc gcggtcgtgt ggcaatacgc tccgtacgcg
     5701 ctcgaagggg gctggccgaa gctgagagac gaatacgcgc ggcacgtgct cgggctctgg
     5761 cagtcctacg caccgaacat gacccgggac aaggtgctcg ccatgaagat ccaggacccc
     5821 accgataccg agcgcctgaa cgacaatctc gtgaacggag tcgacgtggt gggcgacatg
     5881 acgcccgacc agatggggta cttcagacca ttcgcaggct ggtcgagcta ccgcacgccc
     5941 atcgagggcc tgtacatggc cggcggttac tgccatccgg gcggcggcgt gcacgcgggc
     6001 gcggggcaca acgcggcggg cgtcatcgtc gaggacttca agctcaagaa gtggtggggt
     6061 tgatgcccga gcgcgaggcc aggttcaacg tcaacgcgcc gccggcagag ctttggaaat
     6121 tcctgcgcga cttccgcgcg ctctgcacct gcattccggg cgtcgagcgc atccacgtcg
     6181 tggaccagcg cacggccgag ctcacggtga aggagaaagt cggcgtcgta ccgctcaccg
     6241 tgaacctgcg cgccgaaatc gattcggaag aaccgccgcg gcggctgcat gccactgcgc
     6301 gggccgagca tctcacgatg gcgatcgaag tcacgctgcg ggctaccgga tcgggcaccg
     6361 agctcgtgag tcacttcgag gtgaagggcg aggggccgct caagccggtg gtcgaccggc
     6421 tcttcgacaa gcgcgcggcc gagcggaccg cccagttcac ggaatgcatc ggcaaacgct
     6481 tcggcgccgt caccgctccg cgcgagcctc ccctcgggtg gctcgcgcgt ctctggcgtc
     6541 tcgtcagggg gctttcacgg ccttcatcct gatcgacttg tcgagaaact cgttggtgta
     6601 cgtgctcttc acgtcgaagg gcttgtcgac gccgacgtag tcgcgcacca gcgcgtaatc
     6661 cgcctgcatc cttgcgtcgt cgtgccagcc gagcgccacg gtctgcgaga tcttgtcgct
     6721 catcagcact tccacgagct gccagttgac gagctcgttg tcgaagctga gcgcgccgtt
     6781 cgcgtcgatc agcgcctgca cgcagggctt gggctccttc acgcactcgg cgaacgcgcg
     6841 ctgcgtcacc cggacgaact tcgcgaccag cggctgattc gtcttcaggt actcggcgtt
     6901 cacgatcacc gaattgccgt aagggttgag cccgatatcg cgccacgcga ggaagcccat
     6961 gtcggcgccg agctcgcgct ggaagacgtg atggaggttg tagaacgagg tggtgatatc
     7021 gatcgacttc gccttgagcg cggagagctt ggcgttggcg tcgatgttga cccacgtgac
     7081 cgacttcggg tcgatgccgt tcgccttggc gagcgccggc cacatggtgc gcgcgccgtc
     7141 ggcggcgggg ttgccgatct tcttgccggg gaagtccttg atgcccttga tgcccgagct
     7201 tttcaaccag tacaggcctt gcggcgagtt cgcatagacg ttgaacacgg cgacggtgtc
     7261 ggcgcccttg cccttggcga ccagcacgcc gcccatgtcc gcgagaccga ccggattcgc
     7321 gcccgcgccc accttctgtg tcgcgagcgc cgagcccttg ccgggctcga ggttgaggtc
     7381 gatgccttcc ttctggtacc agccgagctt cttcgcgtag tagtacggcg cgtggtctcc
     7441 gcccggcacc cagttgagaa tgaaatcgat tttttgtgcc gcggccggcg tggcagcaaa
     7501 gggtagagac gcggcggcgg cagcgatgat cagcttgcgc atgaaagccc tcctcgggaa
     7561 aaaacgcgcc aatgctacca tcgagtaacc gcccggttaa cgtgacgctc ccttccgaga
     7621 ttgccgcgct gctgcgtcgc ggcaccgtca ttcccgctca tccgctcgcg ctcgacgcgc
     7681 ggcgccagcg cgcgctcacg cgctactacc tcgacgccgg cagcggcggc ctcgccgtgg
     7741 gcgtgcacgc tacgcagttc gcgatccgcg aagccgggct ctacgagcct gtcttgcgaa
     7801 ttgcattgga agaaacgaag acttgggcta agcgcccagt cgtgctgatc gccggcctgg
     7861 ccggaaagac cagcgcggcg aagcgcgagg cgcagctcgc gcgctcgctc ggc
//
LOCUS       DASTPD010000034         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_14429, whole genome shotgun sequence.
ACCESSION   DASTPD010000034 DASTPD010000000
VERSION     DASTPD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14429"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1099)
                     /locus_tag="VFR57_01530"
     CDS             complement(77..1099)
                     /locus_tag="VFR57_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258086.1"
                     /translation="MDYLLPTTVVGSYPQPGWLIDRAKLGSKVPRVRAPEIWRVDKAL
                     LEEAQDDATLLAIRDMERAGIDIITDGEMRRESYSNRFATELEGIDIENPGSTINRSG
                     VSIPVPRVAGPIRRKGPVEVRDVTFLRGNTSRKTKITLPGPFTMAQQAQDDYYKDEEA
                     LALAFAAAVNEELRDLKAAGADVVQIDEPWLQARPERAARYGVKAINRALEGIPGTTV
                     VHLCFGYAAAVKDKPTGYSFLPQLEATSARQISIEAAQPRLDLGVLRDLSKTMMVGVI
                     DLGTNDVETPQEVAGRIRAALKHVPAERLVLAPDCGMKYLKREAAFGKLKARAEGARI
                     VRKELA"
     gene            complement(1172..1456)
                     /locus_tag="VFR57_01535"
     CDS             complement(1172..1456)
                     /locus_tag="VFR57_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258087.1"
                     /translation="MALGVLEVEPLVALPDGDVLVEPLAEDGELGVVVPDAEPDAEPD
                     GELGVVAEPEDEVAPELGVARRSPVLSPQAVSRPAPSAMEIATAKAESLI"
     gene            complement(1543..>2189)
                     /locus_tag="VFR57_01540"
     CDS             complement(1543..>2189)
                     /locus_tag="VFR57_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0258088.1"
                     /translation="VVSTPYDLARHGFTMNAYVADPIEWAAVFKMPDSGPPGLWRLAF
                     PCDPGLPEDTLLDPRGVQARMQGFLPLDHDYEIRYQNIYRVHQRVAADWRRGRVLLAG
                     DAAHLNNPLGGFGLNSGIHDAVNLAEKLAKVWSGEADDSLLERYVRQRRAATIEHVQA
                     MSIRNKRLLEERDPQVHRSRMEELVAIAGDPQRARAYMLESSMIAGLRKSAQIE"
BASE COUNT          348 a          747 c          709 g          385 t
ORIGIN      
        1 aggtcgaccc gaagcaggaa gagcagcaac gcgacttccg catgcggggt gagagcgcgg
       61 gcccgcgcgg cggcggctaa gcgagctcct tccggactat ccttgcgccc tcggcacgcg
      121 ctttcagctt gccgaacgca gcctcgcgct tcaggtattt catcccgcag tccggtgcga
      181 gcaccaggcg ctctgccggc acatgcttca acgcggcgcg gattcggccc gccacctcct
      241 ggggcgtctc aacgtcgttg gtgccgaggt cgatcacgcc caccatcatc gtcttggaga
      301 gatcgcgcag cacgccgagg tcgagcctcg gctgcgccgc ttcgatggag atctggcgag
      361 cgctggtcgc ctcgagctgt ggcagaaacg agtagccggt cggcttgtcc ttcaccgccg
      421 cggcgtagcc gaagcacagg tgcacgaccg tcgttccggg gattccctcg agcgcccggt
      481 tgatcgcctt taccccatag cgcgcggcgc gctcgggtcg cgcctgaagc cacggttcgt
      541 cgatctgtac gacgtccgcg cccgccgcct tcaggtcgcg cagctcctcg ttcacggcgg
      601 cggcaaaagc aagcgcgagc gcttcctcgt ccttgtagta gtcgtcctgc gcctgctgcg
      661 ccatggtgaa cggcccgggg agggtgatct tggttttccg ggaggtattg ccccgcaaaa
      721 acgtcacgtc gcgaacttcg accggtcctt tgcggcgtat cgggccggcg acgcgcggta
      781 ccgggatcga gaccccgctg cggttgattg tcgagcccgg attctcgatg tcgatgccct
      841 cgagctcggt cgcgaagcgg ttggagtagc tctcgcgcct catctcgccg tcggtaatga
      901 tgtcgatccc ggcgcgctcc atgtcgcgga tcgcgagcag cgtggcgtcg tcctgcgctt
      961 cctcaagcaa cgccttgtcg acgcgccata tttccggcgc acggacgcgc ggcaccttgg
     1021 agccgagctt ggcgcggtcg atcagccacc cgggctgggg ataactaccg acgactgtcg
     1081 tgggaaggag atagtccaag ggaactacga tcttaacgcc gcgggcccaa accttgctgc
     1141 atatgcttcc accccaacca cggaggccct ctcagatgag actttcagct ttggcagtag
     1201 caatttccat ggcgctcggc gccggcctgc tcaccgcctg tggggataac acgggcgacc
     1261 gccgcgcgac cccaagctct ggcgcgacct cgtcctccgg ctcggccacg accccgagct
     1321 cgccgtcggg ctccgcatca ggctcggcgt cggggaccac taccccgagc tcgccgtcct
     1381 ccgcgagcgg ctcgacgagc acgtcgccgt cgggcagcgc cacgagcggc tcgacctcga
     1441 gcactccaag cgccacggat gatccgacca aggagaagaa gcagtaagta gcaggtcgca
     1501 cgacaaggcc gtgcttgccc ccgagggcag cgcggccttt tgctattcga tctgcgcgct
     1561 cttgcgcagt ccggcgatca tcgaagactc cagcatgtag gcgcgggcgc gttgcgggtc
     1621 gccggcgatc gccaccagct cctccatgcg gctcctgtgc acctgcgggt cgcgctcctc
     1681 gagcagtcgc ttgttgcgga tcgacatcgc ctggacatgc tcgattgtcg cggcgcggcg
     1741 ctggcgcacg taacgctcga gcagcgagtc gtcggcctct ccgctccata ccttcgccag
     1801 cttctcggcg aggttgacgg catcgtggat gccgctgttc aggccgaagc cgccgagcgg
     1861 gttgttgaga tgtgcggcgt ctccggcgag cagcacccgg ccgcggcgcc aatcggctgc
     1921 gacacgctga tgcacgcggt aaatgttctg gtagcgaatc tcgtaatcat ggtcgagggg
     1981 gagaaaaccc tgcatcctcg cctgcacgcc gcgcgggtcg agcagcgtgt cctccggtag
     2041 cccgggatcg caaggaaagg caagccgcca taaacccggc ggcccggagt cgggcatctt
     2101 gaataccgcc gcccattcga tcggatcagc cacatacgcg ttcatcgtga agccgtgccg
     2161 ggcgaggtcg tacggcgtgc tgaccacga
//
LOCUS       DASTPD010000035        10868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1647941, whole genome shotgun sequence.
ACCESSION   DASTPD010000035 DASTPD010000000
VERSION     DASTPD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647941"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFR57_01545"
     CDS             complement(<1..927)
                     /locus_tag="VFR57_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0258089.1"
                     /translation="MAAATDLLDSRRFGYLKPKDVARLAEAYRFSEAAHHGQTRQSGD
                     PYISHPLAVAEILADWHLDGQTLMAALLHDVTEDTSVTKDEISDTFGKPVAELVDGVS
                     KLDRIEFQSAEDAQAENFRKMLLAMARDVRVMLIKLADRLHNMRTLGAVPPAKRRRIA
                     RETMEIYAPIANRLGLNTLYHELQELAFSHLYPTRYKVLAKATKAARGNRREMIGRTL
                     EAIRKRLTDSGIPAQVFGREKHVYSTYRKMIEKHLSFSEVHDIFGCRVIVKDVPACYL
                     ALGALHALYKPIPGKFKDYIAIAKANGYQSIHT"
     gene            complement(956..1183)
                     /gene="rpoZ"
                     /locus_tag="VFR57_01550"
     CDS             complement(956..1183)
                     /gene="rpoZ"
                     /locus_tag="VFR57_01550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU0258090.1"
                     /translation="MARITVDDCLRRIPNRFQLTLAATYRARQLGAGASPMVEPNRDK
                     ATVIALREIASGKVGTEVLKRGATPSSPPGL"
     gene            complement(1164..1727)
                     /gene="gmk"
                     /locus_tag="VFR57_01555"
     CDS             complement(1164..1727)
                     /gene="gmk"
                     /locus_tag="VFR57_01555"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0258091.1"
                     /translation="MITAPSGAGKTSLIEALIRDDPSLELSISYTTRAPRPGEKNGVD
                     YHFVDDAEFLAMRGRGDFVESAEVHGYRYGTSKRVIADALARGRNLILEIDWQGARQV
                     RAIYPDCIGIFILPPSIEELERRMRSRALDSETVIRRRLANAREELEHSGEFEYRIIN
                     KDFEIAQRELAEIIRKERAKAHGAHHR"
     gene            complement(1753..2622)
                     /locus_tag="VFR57_01560"
     CDS             complement(1753..2622)
                     /locus_tag="VFR57_01560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU0258092.1"
                     /translation="MSLPVQSMTGYAAAAAGSARGTLGLELRSVNSRFLDLQFRIPDE
                     LRAHEPVLRELLTARISRGKVDCRLSLVEGTAQAASRLNSEALARLRALAESAQKAFP
                     EAAPLRLADVLRWPGVIAEPADDESLRAALAALCRLALDELVAARAREGKKLAKVVLE
                     RVRAMHARLDQIAPLMPQAIAAYQEKLAERLREALGSADDERLRTEIAVFAAKVDVAE
                     ELERLRAHLDEVQRVLGAGGPVGKRLDFIAQELNREANTLAAKAASQAISDAALELKL
                     LVEQIREQVQNIE"
     gene            2701..3435
                     /locus_tag="VFR57_01565"
     CDS             2701..3435
                     /locus_tag="VFR57_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEU0258093.1"
                     /translation="MIVQESRIGAREKNEDRVAHWTSPAAVLMAVADGLGGHLHGELA
                     AQIAIDAFGATFAAEARPVVPDPATFLELALAAAHADIVQEAYRQDLPEHPRTVIAAC
                     LVQDGHAYWTHVGDCRFYLLRAGEILARSRDHTVVQKLVDEGRIPEESAGTHPFRNRL
                     LQCLGGYENPRSEPVASAQLASGDILLLCSDGFWGPLGLRQLVQALLSRPLERAVPEL
                     VDAAEAAAGSDCDNISVVAMTWNPPQ"
     gene            3471..4157
                     /gene="rph"
                     /locus_tag="VFR57_01570"
     CDS             3471..4157
                     /gene="rph"
                     /locus_tag="VFR57_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU0258094.1"
                     /translation="MRPVRIQRRYTRHAEGSVLVEFGDTRVLCTASVEERVPPFLKDS
                     GRGWVTAEYGMLPRSTNTRTEREAARGKQSGRTQEIQRLIGRSLRSIVDLASLGPRTV
                     QIDCDVLQADGGTRTAAITGSFVALHDAFSFLREKSLIAAWPLRDHVAAVSVGMVEST
                     ALLDLDYTEDSGCDCDVNVVMTGSGGFVEVQGTAEGEAFSRAELDRLLDLAARGIAQL
                     VQHQKKSLGL"
     gene            4154..4747
                     /gene="rdgB"
                     /locus_tag="VFR57_01575"
     CDS             4154..4747
                     /gene="rdgB"
                     /locus_tag="VFR57_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU0258095.1"
                     /translation="MKLVLASSNPGKLREIGALLAPLSVELISQRSLHIPEADEPHAT
                     FLENALAKARHASRASGLPALADDSGLCVESLSGEPGVHSAYYAGREGSREARDARNN
                     DKLMSILKENRKAYYCCVLVMLRHPEDPRPLVAEGIWRGEIGRAPRGQNGFGYDPLFY
                     LNELGKTAAELDPDHKNRISHRSIALRKLLELLNDQA"
     gene            4734..5948
                     /gene="hemW"
                     /locus_tag="VFR57_01580"
     CDS             4734..5948
                     /gene="hemW"
                     /locus_tag="VFR57_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0258096.1"
                     /translation="MIKLEALPPLALYVHIPWCVRKCPYCDFNSHERTGALPENEYVA
                     KLVLDLERLLPSVWGRRWVSVFIGGGTPSLLSPEAIDRLLSALRARLTLEPEAEITLE
                     ANPGAAEAARFRGFRDAGVNRLSIGVQSFDARMLAALGRIHGPDEARNAIEAACASFD
                     NVNIDLMYGLPGQTLAMAGADIEEAARTGVPHISAYQLTLEPNTVFWSRPPQLPPHDD
                     CADMQMEVESVLGKAGFEHYEISAFAKPGRRCDHNLNYWEFGDYLGIGAGAHGKVSFP
                     DRITRHERVKQPREYMQLDDTLASQRTIPPAELAFEFMLNALRLIDGFPPRLFVERTG
                     LSLLSVEKSLGQAEERGLLERDWKRVRPTARGQRFLNDLLVLFLSDGTPDRAPDGRGG
                     GRVIRISSPAGR"
     gene            complement(5842..6504)
                     /gene="trmB"
                     /locus_tag="VFR57_01585"
     CDS             complement(5842..6504)
                     /gene="trmB"
                     /locus_tag="VFR57_01585"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU0258097.1"
                     /translation="MNRAIRSYVLRQGRTTPAQARALETLFPKYGIAFSREIIGSARA
                     APLILEIGSGMGETTVAIAKAHPEVDFIAVEVHAPGVGSLLNAIEKERLSNLRVIRHD
                     ALEVLEHMIADGTLAAIHLFFPDPWPKKRHHKRRLVQPAFAALAAKKLAPGGVLHAAT
                     DWPDYAHQMEAVFRNERLLEPARAGFTARPVTKFESRGRRLGHPVRDLVFRRKEKAPA
                     GR"
     gene            complement(6501..7301)
                     /locus_tag="VFR57_01590"
     CDS             complement(6501..7301)
                     /locus_tag="VFR57_01590"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU0258098.1"
                     /translation="MSVKDPLIVGGKSYSSRLLIGTGKYRDFAQTRAAVEASGAQIVT
                     VAIRRTNIGQNANEPSLLEALPPSKFTYLPNTAGCYSAEDAVRTLRLARELLEGHDLV
                     KLEVLGDPHTLYPNMPETLKAAEQLVKEGFKVMVYCSDDPIQAQMLEATGCVAIMPLA
                     SLIGSGMGILNPWNLQLVIEKVKVPVIVDAGVGTASDASIAMELGCDGVLMNTAVAAA
                     RDPVLMAKAMRKAVEAGREAFLAGRMPKKLYSASPSSPAEGKIAPKAA"
     gene            complement(7298..7501)
                     /gene="thiS"
                     /locus_tag="VFR57_01595"
     CDS             complement(7298..7501)
                     /gene="thiS"
                     /locus_tag="VFR57_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU0258099.1"
                     /translation="MIQVTVNGAAHRFEQPLEVSALLSTLNLAGRKVAVERNGEIVPK
                     SAHAATLLADGDRLEIVVAVGGG"
     gene            complement(7517..7753)
                     /locus_tag="VFR57_01600"
     CDS             complement(7517..7753)
                     /locus_tag="VFR57_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258100.1"
                     /translation="MGIIQRFIGVLLGLLFVAAVLVFASLALGVLVAVGLVVWGWLWW
                     RTRSLRRAPGRGVVIEGEYRDITPAQRIENRERP"
     gene            7774..7847
                     /locus_tag="VFR57_01605"
     tRNA            7774..7847
                     /locus_tag="VFR57_01605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7806..7808,aa:Gly,seq:ccc)
     gene            complement(7909..9675)
                     /locus_tag="VFR57_01610"
     CDS             complement(7909..9675)
                     /locus_tag="VFR57_01610"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0258101.1"
                     /translation="MAEEGLKHRLTAILAADVAGYSRLMSMDERATVAALDAARAIFR
                     KQIEANQGRIIDMAGDSVLAVFDTAAGAVTAALGVQAALLNSAAAMPEDRRMRFRIGV
                     HLGDVIEKTDGTVYGDGVNIAARLEGLAEPGGVTVSESIRTAVKGRVGAAFEDQGSQQ
                     VKNIADPVRAYRVGPESSAAPKAAAAAGAATRLAPDKPSIAVLPFTNMSGDPEQEYFA
                     DGITEDIITDISKISGLFVIARNSSFVFKNRSIDVKEVGGKLGVRHVLEGSVRKVGMK
                     VRINVQLINAETGGHVWAERYDRGLEDILAVQDEVTRRIVGALEVNLTSSELVRREDL
                     GKVNPQAYDYLIRGRSCILQFTAEALVEGRAMLERALAIDAGLAPAYASLAILHCTEY
                     VNGWNNPGADHLERALELAGKACEVNQFEPASHFARAIALMWLRQLDDAERAAQRAIE
                     LDPNRAEAYGCRGNVLDFAGQHENAIRLFERALRLDPQFDLWIHACGRAQFALERFDE
                     AEASFKRRLIRMPRSDVTRAYLAALYGHTGRHEEARQMWRELMAINPKYSLEHVRRVM
                     PYKSPATLDRLVDGLRKAGLGN"
     gene            10143..10216
                     /locus_tag="VFR57_01615"
     tRNA            10143..10216
                     /locus_tag="VFR57_01615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10175..10177,aa:Gly,seq:ccc)
     gene            10237..>10868
                     /locus_tag="VFR57_01620"
     CDS             10237..>10868
                     /locus_tag="VFR57_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0258102.1"
                     /translation="MDVRAAVAWQAGKPLSIETVQLAGPRAGEVLVEIKATGVCHTDE
                     FTRSGADPEGLFPAILGHEGAGVIVDVGSGVSSVKKGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGVMPDGTSRFSLGKTTIHHYMGCSTFANFTVLPEIAVAKVREDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVVQGARMVGA"
BASE COUNT         1871 a         3484 c         3528 g         1985 t
ORIGIN      
        1 ggtatggatg gactggtagc cgttcgcctt cgcaatggcg atgtagtcct tgaatttgcc
       61 cgggatcggc ttgtacagcg cgtgcagcgc gccgagggcg agatagcagg ccggcacgtc
      121 cttcacgatc acgcggcaac cgaagatgtc gtgcacctcc gagaacgaga ggtgcttctc
      181 gatcatcttg cggtaggtcg agtagacatg cttctcgcgc ccgaatacct gggcggggat
      241 gccgctgtcg gtgagcctct tcctgatcgc ctcgagtgtg cggccgatca tctcgcgccg
      301 gttgccgcgc gccgccttgg ttgccttggc gagcaccttg tagcgcgtgg gatagaggtg
      361 ggagaacgcg agctcctgca gctcgtggta gagcgtgttc aggcccaggc gattggcgat
      421 cggcgcgtaa atctccatgg tctcgcgcgc gatacggcgc cgcttcgccg ggggcacggc
      481 gccgagcgtg cgcatgttgt gcagccggtc ggcgagcttg atcagcatga cgcgcacatc
      541 gcgcgccatg gcgagcagca tcttgcggaa gttctccgct tgggcgtcct cggcggattg
      601 gaactcgatg cggtcgagct tcgagacgcc gtcgaccagc tcggccaccg gctttccaaa
      661 cgtatcggag atctcgtcct tggtcaccga ggtgtcctcg gtgacgtcat gcaggagggc
      721 ggccatcagg gtctggccgt cgagatgcca gtcggcgagg atctccgcga ccgcgagcgg
      781 gtgggagatg taggggtcgc cggactgacg ggtctggccg tggtgcgccg cctccgagaa
      841 gcggtaggcc tcggcgagcc gggcgacgtc cttcggcttc aggtagccga agcgcctgga
      901 atcgaggagg tcggtggcgg cagccatggg gtcagattac ccgaccccgc tattgttaca
      961 acccgggagg cgaagacggg gtagcgcccc gcttcagcac ctcggtgcct accttgcccg
     1021 aagcgatttc gcgcagcgcg atcacggtcg ccttgtcgcg gttcggttcg accatggggc
     1081 tcgcgccggc gccaagctgg cgcgcgcggt aagtcgccgc cagcgtgagc tggaagcggt
     1141 tgggaatcct cctcaggcag tcgtcaacgg tgatgcgcgc catgtgcttt tgctctttcc
     1201 ttccggataa tttccgccag ctcgcgctgc gcgatctcga agtccttgtt aattatacga
     1261 tattcgaact cgccggagtg ctcgagttcc tcgcgcgcat tcgccagccg tcggcggatg
     1321 acggtctcgg agtccagcgc gcggcttctc atgcggcgct cgagctcctc gatcgagggt
     1381 ggcagtatga agatgccgat gcagtcgggg tagatagcgc gaacttggcg tgcgccctgc
     1441 cagtcaatct cgaggattag attgcggccg cgcgcgagcg catcggcgat gacgcgcttg
     1501 gaggtgccgt agcggtagcc atgtacttcc gcgctttcga cgaagtcgcc gcggccgcgc
     1561 atcgcaagaa actcggcatc gtcgacgaag tggtaatcga cgccgttttt ttcgcccggg
     1621 cgcggcgctc gcgtagtgta ggagatcgag agctccaggc ttgggtcatc gcggatcagc
     1681 gcttcgatca gcgaggtttt tcccgcgccc gagggcgccg tgatcacgaa cagccttccc
     1741 gcctttttcg ggctactcaa tgttctggac ctgctcgcga atctgctcta cgagcagctt
     1801 gagctccagt gccgcgtccg aaatcgcctg gctggctgct ttggccgcga gcgtatttgc
     1861 ctcgcggttg agctcctgtg cgatgaagtc caggcgcttg cccacaggcc cgccggcccc
     1921 gaggaccctt tgcacttcgt cgagatgcgc gcgcaggcgc tcaagctcct cggcgacgtc
     1981 cacctttgcc gcaaacaccg cgatctcggt gcgcagccgt tcgtcgtccg ccgagccgag
     2041 cgcctccctc aggcgctcgg caagcttttc ctggtaggcg gcgatcgcct gcggcatgag
     2101 cggggcgatc tgatcgagcc gggcgtgcat cgcgcgcaca cgctcgagca cgaccttggc
     2161 aagctttttg ccctcccgcg cacgcgccgc cacaagctcg tcaagcgcga gccgacacag
     2221 cgcggcgagc gccgcgcgga ggctttcatc gtcggcgggt tccgcaatga cgccgggcca
     2281 gcgcaggacg tcggcgaggc gcagcggggc ggcttccgga aaggccttct gcgccgactc
     2341 ggcgagcgcg cgcagacggg cgagcgcttc tgaattcaat cgggacgcgg cttgtgccgt
     2401 cccctcgacg agcgacagcc ggcagtcaac cttgccgcgt gagatgcgcg cggtgagcag
     2461 ctcacgcagc accggctcgt gtgcgcgcag ctcgtcgggg atccggaact gcaggtcgag
     2521 gaagcgcgaa tttacggaac gcagctccag accgagcgtt ccgcgggcgg agccggcggc
     2581 tgccgcggcg tagccggtca tgctttgaac gggaagcgac aagggcgaga cagctatgat
     2641 aggggagatg gccttccaag ccaacgctcg cgttgcagac cccgccccgt gaaaggtccg
     2701 atgatcgtgc aggaaagccg catcggcgcg cgtgaaaaga acgaggaccg cgtggcccac
     2761 tggacatcgc ccgccgcggt gctcatggcc gtggccgacg gcttgggcgg tcacttgcac
     2821 ggcgagctgg ccgcgcagat tgcgatcgat gctttcggcg ccacgtttgc ggccgaggcg
     2881 cggccggtcg tgcccgatcc ggccacgttc ctcgagctcg cgctggcggc ggcgcatgcc
     2941 gacatcgtgc aggaggccta ccggcaggac cttccagagc atccacgcac ggtgatcgct
     3001 gcctgcctgg tgcaggacgg gcacgcttat tggactcatg tcggcgattg ccgcttctat
     3061 ctgctgcgcg cgggcgagat ccttgcccgc tcgcgtgatc acaccgtggt gcagaagctg
     3121 gtggacgaag gccggatacc ggaggagtcg gccggcacgc atccattccg caaccggctg
     3181 ctccagtgtc tgggcggata cgagaaccca cgcagcgagc cggtggcgag cgcacagctc
     3241 gcgagcggag acatcttgct cctttgctcc gacgggttct ggggtccgct tggcctgcgg
     3301 cagctcgtac aagcgctgct ttcgcgcccg cttgagcgtg cggtcccgga gctggtcgac
     3361 gccgccgagg ccgccgctgg ctctgattgc gacaacatct cggtcgtcgc catgacatgg
     3421 aacccgcccc aatagaggtg aagagagcag gcggacggcg cgcggatgag atgcggccgg
     3481 tacgcatcca gcgtcgctac acccggcatg ccgaaggctc ggtgctggtc gagttcggcg
     3541 atacgcgtgt gctgtgcacc gcgagcgtcg aggagcgcgt gccgccgttc ctcaaggaca
     3601 gcggccgcgg ctgggtgacc gccgaatacg gcatgctgcc gcgctcgacc aatacgcgca
     3661 ctgagcgcga ggcggcgcgc ggcaagcagt ccggccgcac tcaggaaatc cagcgcctca
     3721 taggacgctc gctccgctcg atcgtcgacc tggcgtcgct tgggccaaga acggtacaga
     3781 tcgactgtga cgtgttgcag gccgacggcg gcacccgcac cgcggcgatt accgggtcgt
     3841 tcgtcgctct gcacgatgca ttttcgtttc tgagagagaa atcactgata gcggcttggc
     3901 ctctacgcga tcacgtggcc gccgtctccg tcggcatggt ggagagcact gcgcttctcg
     3961 acctcgatta caccgaagac tccggctgcg actgcgacgt gaacgtggtg atgacgggat
     4021 ccggaggctt cgtggaggtg cagggcacgg ccgaaggcga ggcgttttcg cgcgccgagc
     4081 tcgacaggct gctcgatctc gcggcgcgag gcatcgccca actggtgcag caccagaaga
     4141 aatcgctcgg cctgtgaagc tggtgctcgc gtcatccaac cccggcaagc tgcgggagat
     4201 cggcgccctt ctcgcgccgc tatcggtcga actgatttcg cagcgaagcc tgcatatccc
     4261 ggaggccgac gagccgcacg ccactttcct cgagaacgcg cttgccaagg cgcgccacgc
     4321 gagccgcgcg tctgggctgc ccgcgctcgc cgacgactcg ggcctctgcg tggagtcgct
     4381 gagcggcgag ccgggcgtgc actcggcgta ctacgccggg cgcgagggca gccgcgaggc
     4441 gcgcgacgca cgcaataacg ataaattgat gtctatcttg aaagaaaacc gaaaggcgta
     4501 ttactgctgc gtcctcgtga tgctgcggca tcccgaggac ccgcgcccct tggtggcgga
     4561 gggaatatgg cgaggcgaga tcggtcgcgc gccccgcgga cagaatggct tcggctacga
     4621 cccgcttttc tacctgaacg aacttgggaa aacagcagcc gagctcgacc ccgaccacaa
     4681 gaatcgcatc agccatcgca gcatcgcgct gcgcaagctg ctcgagttgc tgaatgatca
     4741 agcttgaggc actgccgccg cttgcgctct acgtgcacat cccctggtgt gtgcgcaagt
     4801 gcccttattg cgacttcaat tcgcatgaac gaacaggtgc gctgccggag aacgagtacg
     4861 tcgccaagct cgtcctcgac ctcgaacgtc tcctgccgtc ggtgtggggc aggcgctggg
     4921 taagcgtgtt catcggcggc ggcacgccca gcttgctttc gcccgaggcg atcgatcgcc
     4981 tgctttcggc gctgcgcgcg cggctaacgc tcgagcccga agccgagatc acgctggagg
     5041 cgaaccccgg cgcggcagag gcggcgcgct ttcgcggctt ccgcgacgcg ggggtcaatc
     5101 gcctatcgat cggcgtacaa agcttcgacg cacgcatgct cgctgcgctg ggtcggatcc
     5161 acggccccga cgaggcccgc aacgcaatcg aggcggcttg cgcgagcttc gataacgtca
     5221 atatcgatct catgtacggc ctgcccggac agacgctcgc gatggcgggc gcggacatcg
     5281 aggaggcggc gcgcaccggc gtgccgcata tttcggccta tcagctcacc ctcgaaccga
     5341 atacggtgtt ctggagccgg cccccgcagc tcccgccgca cgatgactgc gcagacatgc
     5401 aaatggaagt tgaaagcgtg cttggtaaag cgggattcga gcactacgaa atctctgcat
     5461 tcgctaaacc cggccggcgt tgcgaccaca acctgaacta ctgggagttc ggcgactatc
     5521 ttggcatcgg cgcgggcgcg cacggcaagg tgagcttccc ggaccgcatc acgcggcacg
     5581 agcgcgtaaa gcagccgcgc gaatacatgc agctggacga cacgctcgca agccaaagga
     5641 cgatcccgcc tgcagagctt gcgttcgagt tcatgctcaa tgctctcagg ctgatcgacg
     5701 gattcccgcc gcgcctgttc gtagagcgca ccgggctttc actgctatcc gtagaaaaat
     5761 ccttgggcca ggccgaggaa agaggactct tggagcgcga ctggaagcgc gtgcggccga
     5821 ccgcgcgagg ccagcgcttt ctcaacgacc tgctggtgct ttttctttcc gacggaacac
     5881 cagatcgcgc accggatggc cgaggcggcg gccgcgtgat tcgaatttcg tcaccggccg
     5941 ggcggtgaag ccggcgcgag ccggctcgag gagccgctcg ttgcggaaca ccgcctccat
     6001 ctggtgcgcg tagtcgggcc agtcggtcgc agcgtgcagc accccgcccg gcgcaagctt
     6061 cttcgccgcg agcgccgcga acgccggctg caccaggcgc ctcttgtggt gccgcttctt
     6121 gggccacggg tcgggaaaga agagatggat cgccgcgagc gtgccgtcgg cgatcatgtg
     6181 ctcgagcacc tcgagcgcat cgtgacggat gacgcggagg ttcgaaagcc tctccttctc
     6241 gatggcgttg agcaggctgc cgacacccgg tgcatgcact tcgaccgcga tgaagtccac
     6301 ctcgggatgc gccttggcga tcgcgaccgt ggtttcgccc atgccggagc cgatctcgag
     6361 tattaaaggt gccgcgcgcg ccgagccgat aatctctcgg gaaaacgcga tgccgtactt
     6421 ggggaagagg gtttcgagcg cccgcgcctg tgccggcgtg gttctgcctt gccgcagaac
     6481 gtagctgcgg atcgcccggt tcaagcagcc ttcggagcga tctttccttc ggcgggcgag
     6541 ctcggcgagg ccgagtaaag ctttttcggc atgcggccgg cgaggaacgc ttcgcggccc
     6601 gcctcgactg ccttcctcat cgccttcgcc atcaagacgg gatcgcgtgc ggcggcgacg
     6661 gcggtgttca tgagtacgcc atcgcagccg agctccatgg caattgacgc gtcggaggcg
     6721 gtgcccaccc ccgcgtcgac gatgaccggc accttcacct tttcgatcac gagctgaagg
     6781 ttccaggggt tcagaatgcc catccccgac ccgatgagcg aagcgagcgg catgatcgcc
     6841 acgcagcccg tggcttccag catctgcgcc tggatcggat cgtcagagca gtacaccatt
     6901 accttgaagc cctctttaac cagctgctcg gctgccttca gcgtctcggg catgttgggg
     6961 tagagcgtgt gcggatcgcc aaggacctcg agcttcacga ggtcgtggcc ttcgagcagc
     7021 tcgcgcgcga ggcgcagcgt gcgcacggcg tcctcggcgc tgtaacaccc tgccgtattg
     7081 ggaagatatg tgaacttcga cggcggcagt gcttcgagca ggctcggctc gtttgcgttc
     7141 tggccgatgt tggtgcgccg gatcgcgacg gtcacgatct gggcacccga cgcttccacc
     7201 gcggcgcgcg tctgcgcgaa gtcgcgatac tttccggtgc cgatgaggag gcgcgacgaa
     7261 tagcttttgc caccgacgat caatggatcc ttcacgctca tccccctcct accgccacca
     7321 cgatctcgag ccggtcgcca tcggcaagca gcgtggccgc gtgcgcactc ttcggaacga
     7381 tctcgccatt gcgctcgacc gcgaccttcc tcccggccag gttcagcgtc gagagcagcg
     7441 ccgacacttc gagcggctgt tcgaagcgat gcgccgcgcc gttcaccgtg acctggatca
     7501 tgcggtaatg ataacgctaa ggtctttcgc ggttctcaat gcgctgcgca ggcgtgatgt
     7561 cgcggtattc gccctcgatg acgaccccgc gcccgggtgc ccggcgcagc gagcgcgtgc
     7621 gccaccacag ccatccccag actaccaggc ccaccgccac cagcacgccg agggcgagcg
     7681 acgcaaacac caagacggcg gcgacgaaca gcaggccgag caaaacccct atgaagcgct
     7741 ggatgattcc cattcgaata tgattggact tccgcgggtg tagttcaatg gcagaacatc
     7801 agcttcccaa gctgaatacg tgggttcgat tcccatcacc cgctccagcc gttccctaga
     7861 tgccgcctga gacgtcgggc accgcgcccg tggtgtagcg gctgcttgtt agttcccgag
     7921 ccccgctttg cgcaggccat cgacgagccg gtcgagagtc gccgggctct tatagggcat
     7981 gacccgccgt acgtgttcca acgagtactt ggggttgatt gccatcagtt cccgccacat
     8041 ctggcgcgcc tcttcatgcc ggccggtatg gccgtagagc gcggcaagat aagcgcgcgt
     8101 tacgtccgat cgcggcatgc ggatcaggcg gcgcttgaac gacgcctcgg cctcgtcgaa
     8161 gcgttccagt gcgaactggg cccggccaca agcgtggatc cataggtcga actgcgggtc
     8221 aagccgtaac gcccgctcaa agagcctgat cgcgttctcg tgctggccgg caaaatccaa
     8281 tacgttgccg cggcacccat aggcttcggc ccgattggga tcgagttcga tggcccgctg
     8341 cgcggctcgc tctgcgtcgt caagctgccg cagccacatc agggcgatgg cacgcgcaaa
     8401 atgcgaagcc ggttcgaatt ggtttacttc gcaggccttg ccagctagct cgagggctcg
     8461 ttcgagatgg tccgccccgg ggttgttcca accattcacg tactcggtgc agtgaaggat
     8521 cgccaaactc gcgtaggccg gcgccaatcc cgcgtcgata gcaagggctc gttccagcat
     8581 cgcgcggccc tcgacgagcg cttcggcggt gaattgcaag atacagctcc tgccgcgaat
     8641 cagataatcg taagcctgcg gattcacctt gccgagatct tctcggcgca ccagctcgct
     8701 gctggtgagg ttgacctcga gcgcgccgac gattctgcgc gtcacttcgt cttgcaccgc
     8761 aaggatgtcc tccagaccac ggtcgtagcg ttcggcccag acgtgaccgc cggtttcggc
     8821 gttgatgagc tgcacattga tccggacttt catgcccact ttgcggacgc tgccttccag
     8881 cacatgtctt acgcccagct tcccgccgac ctccttgacg tcgatgctcc ggttcttgaa
     8941 cacgaaagac gaattgcggg caatgacaaa aaggccggat atcttggaga tatcggtaat
     9001 gatgtcttcc gtgatgccgt ccgcgaagta ctcctgctcc gggtcgccgc tcatgttggt
     9061 gaagggcagc accgcgatcg acggcttgtc cggggctaaa cgcgtcgccg cgccggcggc
     9121 agccgccgcc ttcggagcgg cactgctttc agggccgacc cggtaggcgc gtaccggatc
     9181 agcgatgttt ttcacctgct gcgagccctg gtcctcgaag gcggcgccga ctctcccctt
     9241 gaccgcggtg cggatcgatt ccgaaaccgt gacgccgcct ggctcggcta gaccttccag
     9301 acgcgcagcg atgtttacgc catcgccata aaccgtcccg tcggtctttt caatgacgtc
     9361 gccgaggtgt accccgatcc gaaagcgcat gcggcggtcc tcgggcattg cagcggccga
     9421 attcagaagc gccgcctgaa cgccaagcgc tgccgtaacc gcacccgcgg ccgtgtcgaa
     9481 cacggcgagc acggaatctc cggccatatc gatgatgcgg ccttggttcg cttcgatctg
     9541 cttgcggaat atcgctctcg cggcatcgag agcggcgaca gtcgctcgct catccatcga
     9601 catcaggcgc gagtagcccg cgacatcggc ggccaatatc gccgtaagcc ggtgcttaag
     9661 accctcctcg gccacagatc tgatcgcctc ctcgaaccta gatgtacggg gatactacct
     9721 cgacgccgct agcgcctcag aatgtccgct ttgggtggcg gacattagag cgcaaagtgg
     9781 tctaaccgat ccgctccccg gcaggtcagc agcttccttt cgaggcatgt atacagccaa
     9841 gagaggtgtt aggtggctgg gtttcggaaa agggacactc gcgttcattc gcagacggga
     9901 gaattcgtta agggcccgta agagtactct gcccaccgca gaggtcagtc gcgttgtggt
     9961 gcgtggagga ctccttagaa tccgcggcgt taggcgggcc ggaatcgaac gcgtattcag
    10021 cttcccaagc ggcaacccac atcagcagcg ttccttgcta ggacggtcgg cacggtaccg
    10081 atatcagtct gtcgggcgtc gttttccacg acgctcggag caccttagaa tcctttctgt
    10141 cagcgggtgt agttcaatgg cagaacatca gcttcccaag ctgaatacgt gggttcgatt
    10201 cccatcaccc gctccaactt cttgggagag ctcgtcgtgg atgtacgcgc ggccgtagcc
    10261 tggcaagcag gcaagccgct ctcgatcgaa acggtgcagc tcgccgggcc acgggcgggc
    10321 gaagtgctgg tcgagatcaa ggcgaccggt gtctgccaca ccgacgagtt cacccgctcc
    10381 ggcgccgatc ccgagggcct tttccccgcg atccttggac acgagggcgc cggggtaatc
    10441 gtcgatgtcg gctcgggagt gagcagcgtg aagaagggcg accacgtcat tccgctctat
    10501 acgcctgaat gccgccaatg caagtcgtgt ctttcgcgca agaccaacct ctgcacggcc
    10561 atccgcgcta ctcagggcaa gggcgtcatg cccgatggca cgagcaggtt ctcgctcggg
    10621 aagacaacaa tccaccatta catgggctgc tcgacattcg cgaacttcac cgtgctgccg
    10681 gagatcgcag tggcgaaggt ccgtgaggac gcaccctttg aaaaggtctg ctacatcggc
    10741 tgcggcgtga ccaccggaat cggcgcggtg atcaacaccg ccaaggtcga gccgggcgcg
    10801 aatgttgtcg tctttgggct gggcggcatc gggctcaacg tggtgcaggg tgcgcgcatg
    10861 gtcggcgc
//
LOCUS       DASTPD010000036         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1649208, whole genome shotgun sequence.
ACCESSION   DASTPD010000036 DASTPD010000000
VERSION     DASTPD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649208"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFR57_01625"
     CDS             complement(<1..393)
                     /locus_tag="VFR57_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0258103.1"
                     /translation="MFRGTPRERVAALARQSLAAQAPAGSAIVCRGERVPGLMVVVEG
                     LAKLALKGDSERVLRFVGPGETFGEAALVLGQPLPIDAVAVTDTSLVVVPAAPLLALF
                     DRDRSFARELLATLCRRLQAMVADFESAT"
     gene            complement(518..1411)
                     /locus_tag="VFR57_01630"
     CDS             complement(518..1411)
                     /locus_tag="VFR57_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0258104.1"
                     /translation="MIDLVITQRRRDLGGFEVGRVLPFAKRRMVGPFIFFDHMGPVNF
                     PPRIPRSVDVRPHPHIGLATLTYLFAGEIMHRDSVGSEQPIRPGEVNWMTAGRGITHS
                     ERFERARREGGRMDGIQAWIALPKDKEETDPGFSHLEEADLPRFQEHGRSIRLIAGEA
                     FGTKASVPVYSPLFYLHCELAPDAVVDLGNDYPERAAYVASGSVAADGQRFDAGQMLV
                     FKPNAKATVRAFTQATLMLLGGEPVGERFIEWNFVSSSRERIEQAKADWRAGRMKLPD
                     LDQEEFIPLPPDPVAPPNPMS"
     gene            1464..2588
                     /locus_tag="VFR57_01635"
     CDS             1464..2588
                     /locus_tag="VFR57_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258105.1"
                     /translation="MSAAAGWIEHQIEDGADVLYLEGPWRLSNLTAIVGALRSLRLRA
                     SRFVLDGSRLQALDTAAGFTLYSHLARLGCTEAMVDERGFDPRHERLLKLVHERMKCP
                     PVAARSVHLGLVQRVGAAAISVWRLTRSHTGFVGALTVEAISLVPKPRRFRLRETVSQ
                     FESAGLDAIPIVGLVTFLIGVVFAYLLGQQALRYGANIFVVDGVGLAMCRELSPMLTA
                     IIVAGRSGAAFTAQIGTMKVQEEIDAIATLGLSPVQVLVIPRLVAITVALPLLVFIGD
                     VCGIAGGMLVAGWQLDVPPRAFIDRLHDVLPLSALVVGLVKAPVFAAFIAVIACRMGM
                     LVARDARSVGVNTTSTVVQSVVSVIVVDAVFAIVFQHLGI"
     gene            2588..>3300
                     /locus_tag="VFR57_01640"
     CDS             2588..>3300
                     /locus_tag="VFR57_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0258106.1"
                     /translation="MRPVLEVESVVTRFGAEMVHEGVSFSVQRGEVVALIGASGTGKS
                     VLLKEIIGLVRPAAGRIRLLGTDVWDANAAALNELRRRFGMLFQDGALFSSLTVAENI
                     AVPFRENTDLPMDIIAPLCGFKLALVGLPPDAGRKTPAQLSGGMRKRAALARALALEP
                     EILFLDEPTSGLDPVGARAFDSLVRLLSDSLGLTVFIVTHDLDTLLSIIDRTIVLSAG
                     KVIADGPVSVVMKSGERWIR"
BASE COUNT          512 a         1105 c         1107 g          576 t
ORIGIN      
        1 ggtggcagac tcgaaatcgg caaccatggc ctgcagccgg cgacatagcg tcgcgagcag
       61 ctcgcgcgca aaggaccggt cgcggtcgaa aagcgcaagg agcggcgccg cgggcaccac
      121 gacgagcgag gtatcggtta ccgcaacagc gtcgatcggc agcggctgac caaggacgag
      181 cgccgcctcg ccaaaagttt cccccggacc cacgaagcgc agcacccgct cggagtcgcc
      241 tttcagcgcg agcttcgcga ggccctcgac caccaccatc agccccggaa cacgctcgcc
      301 gcggcacacg atcgcgctgc ctgcgggcgc ctgcgcggcg agcgattgtc gcgccagcgc
      361 ggcgacccgc tcgcgcgggg tgccgcggaa catgggcaaa cggcaaagca gcgctatcgc
      421 gctgccagcc gctggttcgg gccgtaagct tctcgcacgc cgggccaccc gggaagtctc
      481 cgcccgcggc ggagacgcga cttgatgtaa gtcaagctca ggacataggg ttcggcggcg
      541 ccaccgggtc gggcggcagg ggaatgaact cctcttggtc gaggtcgggc agcttcatgc
      601 gcccggcgcg ccagtcggcc ttcgcctgct cgatccgctc gcgcgaggac gagacaaagt
      661 tccactcgat gaaacgttcg cccaccggct caccgccgag gagcatgagg gttgcctgcg
      721 taaacgcccg gacggttgcc tttgcgttcg gtttaaaaac cagcatctgg cccgcgtcga
      781 agcgctgccc gtcggccgcg actgaaccgg aagccacata ggccgcgcgt tcggggtagt
      841 cattgccgag atcgacgact gcgtcgggcg ctagctcgca gtgcaggtag aaaagcggcg
      901 agtacaccgg caccgacgcc ttcgtgccga aggcctcgcc tgcgatgagg cggatggatc
      961 ttccatgctc ctggaaccgc ggcagatccg cctcttccag atgtgagaac cccggatctg
     1021 tctcctcctt gtcctttggc agcgcaatcc aggcctggat gccgtccatg cgtccgcctt
     1081 cgcgccgcgc gcgctcgaag cgctcggaat gcgtgatgcc gcgcccggcg gtcatccagt
     1141 tcacttcgcc cgggcggatc ggctgttcgg agccgacgct gtcgcggtgc atgatctcgc
     1201 ccgcgaaaag gtaggtcagg gtggcgagcc cgatgtgcgg gtgcggccgc acgtccacgc
     1261 tgcgtgggat gcgaggcggg aagttcaccg gccccatgtg gtcgaagaag atgaatgggc
     1321 ccaccatgcg ccgctttgca aacggcagca cgcggcccac ctcgaagccg ccgaggtcgc
     1381 ggcggcgctg agttatgaca aggtcgatca tgcccgcata atgccgcatt cgagttagcc
     1441 cttgttcggg gtaagccagg aatatgagcg cggcggcggg ctggatcgag caccagatcg
     1501 aagatggcgc ggacgtcctt tacctcgagg gcccctggcg gctttccaac ctcactgcca
     1561 ttgtgggcgc gttacgctcg ctcaggctgc gcgcaagccg cttcgtgctg gacggcagcc
     1621 gcctgcaggc gctcgacacg gccgccggct tcacgctcta cagccacctc gcgagactgg
     1681 gctgcaccga ggccatggtg gacgaacgtg gctttgatcc tcgccacgaa cggcttttga
     1741 aactcgtgca cgagcgcatg aaatgcccgc cggtggcggc gcgttccgtg caccttgggc
     1801 tggtgcagcg ggtgggcgcg gcggcgatca gcgtgtggcg gctcaccagg tcgcacaccg
     1861 gcttcgtcgg cgccctgacg gtcgaagcga taagcctggt gcccaaaccc aggcgcttcc
     1921 gccttcgcga gaccgtgtcg cagttcgagt ccgcgggcct cgacgccatc ccgatcgtcg
     1981 gccttgtgac cttcctcatc ggtgtggtgt ttgcctatct cctcggccag caggcgctgc
     2041 gctacggggc caacatcttc gtcgtggacg gcgtcggtct cgccatgtgc cgcgagctct
     2101 cgccgatgct taccgccatc attgtggcgg ggcgctcggg cgccgccttc acggcacaga
     2161 tcggcaccat gaaggtgcag gaggagatcg acgccattgc cacgctcggc ttatcgccag
     2221 tgcaggtcct ggtgatccca cgcctcgtcg ccatcacggt ggccttgccg cttcttgtgt
     2281 tcatcggcga cgtttgcggc atcgccggcg ggatgctcgt ggccggctgg cagctcgacg
     2341 tgccgcctcg ggccttcatc gaccgcttgc acgatgtgtt gccgctctcc gcacttgtcg
     2401 taggcctggt gaaggcgcct gtgttcgcgg cgttcatcgc cgtgatcgca tgccgcatgg
     2461 ggatgctcgt ggcacgcgac gcgcgcagcg tcggcgtgaa tacgacttcc acggtggtgc
     2521 agagcgtcgt ttccgtgatc gtcgtcgacg cggtgttcgc catcgttttt cagcacctgg
     2581 ggatctgatg agacccgtgc tcgaggtcga gtccgtcgtc acgcgtttcg gagccgagat
     2641 ggtgcacgaa ggagtgagct tcagcgtgca gcgcggcgaa gtggtggctc tcatcggcgc
     2701 cagcggcacc ggcaagtcgg tgctgctcaa ggagatcatc ggcctcgtgc gccccgcggc
     2761 cggccgcatc cggttgcttg gcaccgacgt gtgggacgca aacgccgcgg cgctcaacga
     2821 gctgcgccgg cgcttcggca tgctgttcca ggacggagcg ctcttctcct cgcttacggt
     2881 cgcggaaaat atcgccgtgc cgttccgcga gaacaccgac ttgccgatgg acatcatcgc
     2941 gccgctctgt ggattcaagc tcgccctggt cggtctgccg cccgacgcgg ggcgcaagac
     3001 cccggcgcag ctttccggcg gaatgcgcaa gcgcgccgcg ctcgcgcgcg cgctggcact
     3061 ggagccggaa atcctcttcc tggacgagcc gacgtcgggg ctcgatccgg tcggcgctcg
     3121 cgccttcgac agtctggtgc ggctgctctc cgacagcctc gggctcaccg tgttcatcgt
     3181 cacgcacgac ctggacacgc tgctttcgat catcgaccgc acgatcgtcc tgtcggcggg
     3241 caaagtgatc gccgatggcc cggtcagcgt cgtcatgaag tcgggcgagc gctggatccg
//
LOCUS       DASTPD010000037         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_221001, whole genome shotgun sequence.
ACCESSION   DASTPD010000037 DASTPD010000000
VERSION     DASTPD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221001"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFR57_01645"
     CDS             complement(<1..235)
                     /locus_tag="VFR57_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0258107.1"
                     /translation="MIAIEVNGARHSVTAAPETPLLYVLRNHLGLMGTRFGCGSGQCG
                     ACFVLVDGRAMASCDMPLSLAVNRKITTIEGLNR"
     gene            270..515
                     /locus_tag="VFR57_01650"
     CDS             270..515
                     /locus_tag="VFR57_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258108.1"
                     /translation="MTDYWLSKMFFDLQSPAAAAEYRADREKVLARYNLEPDVKQAVL
                     ANDVSYLSKRTNPYLLRYFFFAAGMKDDEFIRKLRDG"
     gene            508..1335
                     /locus_tag="VFR57_01655"
     CDS             508..1335
                     /locus_tag="VFR57_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
                     /protein_id="HEU0258109.1"
                     /translation="MGSLVGAYAASHGPLLVREWESLPEREKQRLKSAFERLGQRMAA
                     AKPEVLIVVSPDHWSNFFLDNVPAVCIGVGGTNEGPPEPWMKAFPHREIPGHPALALH
                     IVSEVMKQGFEPSVSHRLKLDHGVCIPLWRMGLERLPRIVPMLVNSIEPPLPSLSRCL
                     AWGRQLAGAISSYAEPLRVAILATGGLSHSIGEPTMGAIYEDFDRETLRLFSSGERDL
                     VSYLEKKLPTHGNGSEEVRNWLVAHGAAGARGFELVDYLPVPKVIVGCGFASWTIPA"
     gene            1567..1809
                     /locus_tag="VFR57_01660"
     CDS             1567..1809
                     /locus_tag="VFR57_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258110.1"
                     /translation="MDKVRRLCRTLASSLGGEVIVEAPIATGMGVFAPDAAWASATGA
                     EEVWIVYPQSKRCESFARGGRMERSAFAVDLADIFK"
     gene            1816..2730
                     /locus_tag="VFR57_01665"
     CDS             1816..2730
                     /locus_tag="VFR57_01665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0258111.1"
                     /translation="MKACFFRRYGAPEVLEYGELPDPAPAAGEALVAVHAASVNAADW
                     KMRAGQYGASISFPHVPGRDFAGVVVEGAGDFKPGDAVFAVCEVPREGAYAERIAIRE
                     SIVARKPEKLTHVQCAAVALAGLTAMVSLEETLKLKRGETILIHGGAGGVGGFAVGLG
                     KHLGARVIATASAANHDYVRSLGANEVIDYRAQDFTRRVADVDAVFDTAGGDVTTRSF
                     AVLRSGGRLATVAAGASAPASPRPDVTSLRPNVKRERRSLQRVTELVTSGAVPLPEIV
                     EYPLAKAAEAHRLSEARHLRGKLVLRVL"
     gene            complement(2710..>4635)
                     /locus_tag="VFR57_01670"
     CDS             complement(2710..>4635)
                     /locus_tag="VFR57_01670"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0258112.1"
                     /translation="PAAVSVEDAAKEGAPKVWDDNTMGNLAFPLMMGNKEATEAAFAK
                     AKHTVSVRLYNNRITANAMEPRAAIGDFANDSFTLYTSSQNPHGARSILANAIFRIPE
                     TKLRVISPDVGGGFGMKGDVYPEDGLVLWASRRLGRPVKWVATRTESLLGDNHGRDQL
                     ISAEMALDENGKILGIRAQALHAVGAYVTNAGVVPVLCGLRNIPNVYVVPAMLVAAKA
                     TFTHTTPLGPYRGAGRPEASYVTERLMDEAARKLDMDPVEIRRRNYIPPSAMPYNTTA
                     NWTVGAAVGWTYDSGEFARLTDRCLELADWSNYSSRKRESEKKGKLRGRAFIYYLEDS
                     GVFNERMELRFDPSGMVTIVAGTHSHGQGHATTYAQLVSDWLGVAFENIRLVQGDTDA
                     VSFGRGTYASRSAMLGGSALRAAADGIIEKAKPLAAHLMEASPKDLEFQAGKFTVVGT
                     DRSIALADVAKASYRPVGPTTKFGIGLDASGSSVAPPTFPNGCHACEVEIDPETGVVE
                     VDRYAVVDDVGRVINPMICHGQIEGALAQGIGQALMENVVFDRQSGQMLSASFTDYAM
                     PRAADLPHHYELDFIDVPAKTNPLGVKGIGEAGCVGAPPAVMNAILDALRSRGVTHLD
                     MPATPRRVWEALQNAKH"
BASE COUNT          751 a         1498 c         1528 g          858 t
ORIGIN      
        1 cacggttcag accctcaatc gtggttattt tcctgttgac cgcgagagac aatggcatat
       61 cgcacgacgc catggcgcgg ccgtcgacca gcacgaagca tgcgccgcac tgcccgctgc
      121 cacagccgaa gcgcgtgccc atgagtccga ggtggttgcg caggacgtag agcagtggcg
      181 tttccggtgc cgcggtaacg ctatggcggg cgccgttcac ttcgatcgcg atcatcgcca
      241 aaacattttg gcataaactc gaccgcgcga tgaccgacta ctggctgtcg aaaatgtttt
      301 tcgacctgca gagcccagcg gccgctgccg agtaccgcgc ggatcgcgag aaggtcctgg
      361 cacgatacaa ccttgagccc gacgtaaagc aagcggtgct cgccaacgac gtctcctacc
      421 tttccaagcg caccaacccg tacttgttgc gctacttctt tttcgccgcc gggatgaagg
      481 acgacgaatt catccgcaag ctgcgcgatg ggtagcctgg tcggcgctta cgcggcgagt
      541 cacggtccgc ttctcgttcg ggagtgggaa tccctgccgg agcgcgagaa acagaggctc
      601 aagagcgctt tcgagcggct cgggcaacgc atggccgcgg cgaagcccga ggtgctgatc
      661 gtggtttcgc cggatcattg gtcgaatttc ttcctcgata acgtgcccgc ggtctgcatc
      721 ggcgtcggcg ggacgaatga aggtccgccc gagccgtgga tgaaggcgtt tccgcaccgg
      781 gaaatcccgg gtcacccagc gctcgcgctt cacatcgtga gcgaggtgat gaagcagggc
      841 ttcgagccct cggtgtcgca ccggctcaag ctcgaccacg gcgtgtgcat tccgctctgg
      901 cgcatggggc tcgagcgcct gccgcgcatc gtgccgatgc tcgtcaacag catcgaaccg
      961 cccttgccct cgttatcgcg ctgcctcgca tggggccggc agctcgccgg tgcaattagt
     1021 tcttatgcag agcccctgcg tgtcgccatt ctggcgaccg gcggcctgag ccactcgatc
     1081 ggcgagccga cgatgggcgc gatctacgag gacttcgacc gcgagaccct ccggcttttc
     1141 tcttccggcg agcgcgacct ggtctcttat ctcgagaaga agctgcccac tcacggcaac
     1201 ggctcggagg aggtgcgcaa ctggctcgtc gcgcacggcg ccgctggagc gcgcggcttc
     1261 gagctggtcg actatctgcc ggtgccgaaa gtgatagtgg gttgcggttt cgcctcctgg
     1321 actattccgg cttgacgccg gcgacgcggg ccatctcccg taggccttca gctcggcgag
     1381 gaaaaacttg cggaattcct cgggtgtgct cgccgctgac tcgacgccga ggctggcgaa
     1441 gcgctcgagg accgcgggtt tcttcatcgc ggtgcgcacc tcggccgctc ctccgttgca
     1501 gctcagtgta cgtcaacgta gtggcaacac gtacgttcct cgggcgggga gcgtaagatg
     1561 gcttgcatgg acaaagtgcg ccgcctgtgt cgaacgctcg catcaagtct cggcggggaa
     1621 gtcattgtag aagcacccat agcaaccggc atgggagtgt ttgcgcccga cgctgcatgg
     1681 gccagcgcca cgggcgccga agaggtctgg atcgtctacc ctcagtccaa gcgctgcgag
     1741 tccttcgcca gagggggccg gatggagcgc tcggctttcg ccgtcgatct ggccgatatc
     1801 ttcaagtagt caggtttgaa agcctgtttt tttcgtcggt acggcgctcc ggaggtcctc
     1861 gaatatggcg agctgccgga tccggcgccg gcggccggcg aggcgctggt cgcggtgcac
     1921 gcggcgagtg tcaacgccgc cgactggaaa atgcgcgccg gacaatacgg cgcgtcgatc
     1981 tcgtttccgc atgttccggg gcgcgatttt gcgggcgtcg tggtcgaggg cgcgggtgat
     2041 ttcaagccgg gcgacgcggt gttcgcggtg tgcgaagtgc cgcgcgaagg cgcgtacgcc
     2101 gagaggatcg ccattcgcga aagcatcgtc gcgcgcaagc cggagaagct cacgcatgtg
     2161 caatgcgcgg ccgtcgcgct cgccggcttg acggcgatgg tgtcgctcga agaaaccctg
     2221 aagctaaagc gcggtgaaac gatccttatc cacggcggcg cgggtggggt cggcggcttc
     2281 gcggtcggcc tcggaaaaca ccttggcgcg cgcgtcatcg caaccgcgag cgcggcaaat
     2341 catgactatg ttcgctcgct cggcgcaaac gaggttatcg attaccgcgc ccaggatttc
     2401 accaggcggg ttgccgatgt ggatgcggtg ttcgacacgg ccggcggcga cgtcaccacg
     2461 aggagtttcg cggtgctgcg ctccggcggc cggctcgcca cggtggctgc cggcgcgagc
     2521 gcgcccgctt cgccgaggcc cgacgtaact tcgctacgtc cgaacgtgaa acgcgagcgg
     2581 cgctctcttc agcgagtcac cgagctggtc acctcgggcg cggtgccgct gccggagatc
     2641 gtcgagtatc cgctcgccaa agcagccgaa gcccaccgcc taagcgaggc gcgccatctg
     2701 cgtggaaagc tagtgcttcg cgttctgtaa agcttcccaa acccggcgcg gggtcgccgg
     2761 catgtcgaga tgcgtgacgc cccgcgaacg caacgcatcc aggatggcgt tcatcaccgc
     2821 gggcggcgcg ccgacgcagc ccgcttcgcc gatgcctttc acgccgagcg ggttggtctt
     2881 tgccggaacg tcgatgaagt cgagctcgta gtggtgcggc aggtccgctg cacgcggcat
     2941 cgcgtagtcg gtgaagctcg ccgacagcat ctgtcccgat tgacggtcga acaccacgtt
     3001 ctccatgagc gcctggccga tcccttgcgc aagcgcgccc tcgatctggc cgtggcagat
     3061 catcgggttg atcactcttc cgacgtcgtc caccaccgcg tagcggtcca cctcgaccac
     3121 tccggtttcg gggtcgatct cgacctcgca ggcgtggcag ccgttcggga aggtcggcgg
     3181 cgcgaccgat gacccgctcg cgtcgaggcc gatgccgaac ttcgtcgtcg gcccgaccgg
     3241 gcgataggag gccttcgcca cgtcggcgag cgcgatcgag cggtcggtgc cgaccacggt
     3301 gaacttgccc gcctggaatt ctagatcctt cggagaagct tccatgagat gcgctgcaag
     3361 cggcttcgcc ttctcgatga tgccgtcggc cgcagccctg agcgccgaac cgccgagcat
     3421 cgcgctgcga gaggcgtacg tgccgcggcc gaacgacacc gcgtcggtgt cgccctggac
     3481 aaggcggatg ttctcgaacg caacgccgag ccagtcggac acgagctgcg cgtaggtggt
     3541 ggcgtgcccc tggccgtggc tgtgcgtgcc ggccacgatc gtcaccatgc cgctcggatc
     3601 gaagcgcagc tccatgcgct cattgaacac gcccgagtcc tccaggtaat agatgaaggc
     3661 gcggccgcga agctttccct ttttttctga ttctcttttt cgggatgaat agttggacca
     3721 atcggcaagc tcgagacaac ggtccgtcag ccgggcgaat tcgcccgagt cgtaggtcca
     3781 tcccacggcc gcgccgacag tccagttggc cgtcgtgttg tacggcatgg cgctgggcgg
     3841 aatgtagttg cgccggcgga tctccaccgg atccatgtcg agcttgcgcg ccgcctcgtc
     3901 catcagccgt tcggtcacgt acgacgcttc cgggcggccg gcaccgcggt agggtccaag
     3961 cggcgtggtg tgggtgaaag tcgccttggc cgcgaccagc atcgccggca ccacatagac
     4021 gttcgggata ttgcgcagtc cgcagagcac cggcactacg ccggcgttgg tgacatacgc
     4081 gcccaccgcg tgcagcgcct gggcgcgaat gccgaggatc ttgccgttct cgtcgagcgc
     4141 catctcggcg ctgatcagct ggtcgcggcc gtggttgtcg ccgagcagcg attcggtgcg
     4201 cgtcgcgacc catttcaccg gccgtcccag ccgccgcgat gcccagagca cgagcccgtc
     4261 ttccgggtac acgtcgccct tcatgccgaa gccgccgccg acgtcggggg agatcaccct
     4321 gagcttcgtt tccgggatcc tgaaaatcgc gttggcaagg atcgagcgcg cgccgtgcgg
     4381 gttctgcgag ctcgtataga gggtgaagga atcgttcgcg aaatcgccga tggcggcgcg
     4441 cggctccatg gcgtttgccg tgatccggtt gttgtagagc cgcacggaaa ccgtgtgctt
     4501 cgctttcgcg aacgccgcct ccgtcgcctc cttgttcccc atcatgagcg gaaaggcgag
     4561 gttgcccatc gtgttgtcgt cccacacctt gggcgcgccc tccttcgcag cgtcctcgac
     4621 gctcaccgct gccgg
//
LOCUS       DASTPD010000038         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1242171, whole genome shotgun sequence.
ACCESSION   DASTPD010000038 DASTPD010000000
VERSION     DASTPD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242171"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..809
                     /locus_tag="VFR57_01675"
     CDS             138..809
                     /locus_tag="VFR57_01675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0258113.1"
                     /translation="MPKVEIRREIVERVLARRGRLHLFDSLDPKRSALLVVDMQNAFV
                     APGAPIEVPAARGIVAPINQLAAELRHRGVTIVWVAHENRADGRDWAGFFDAFVAPGR
                     RAEAAAALAGGSKLQQLWPELDVNPGDLRVSKNRYSPLIGNSSLEKLLREKGIDTLLL
                     AGTKTNVCVECTARDAMMLDFKVVLLSDCTASLSDDEQRATLENVIQQFGDVRTAEEA
                     LALFT"
     gene            806..1174
                     /locus_tag="VFR57_01680"
     CDS             806..1174
                     /locus_tag="VFR57_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0258114.1"
                     /translation="MITHTGGCHCGRVRFEVQAPAKLDVLECNCSVCSKAGYLHLIVP
                     ADRFKLLSGKDMLTGYGFNTHTAKHLFCSVCGIKSFYVPRADPNSRSVNVRCLDPGTV
                     EGMTITQFNGRDWGRRGYSP"
     gene            complement(1180..>2263)
                     /locus_tag="VFR57_01685"
     CDS             complement(1180..>2263)
                     /locus_tag="VFR57_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0258115.1"
                     /translation="TDAEFYGEFCVSGHDGIAEYWRSGGVTGQPLFYPRSFRDIEYGL
                     EAFARTFDCAGAKPGDRAHVSFPMGIHPVGQIFARCAGSRGVTVNWAGAGTSTPSALQ
                     IELIRRLKPTIWLGMSSYALHLANLAEARGLELRIDKLMTSAEPVSEAKRVKIERSWG
                     AKLIDTFGMTEAGMMGAEDKPGAGFRIWTDMYYIEVLDPKTLEPAREGEIGTLVVTAL
                     WANNITPFLRWSSGDLVTYSEADDGEGPFSVFPRIKHAHRTAGFFKIRGVNLGHQDLE
                     DFMFRHLEIGDFRAEAVNEGGNDILRVLIETKKGVDSAAVSRALAEGIKHTFELAPQI
                     VVLETGTLAREFEANVKAARFIDRRG"
BASE COUNT          402 a          740 c          728 g          393 t
ORIGIN      
        1 atggatcgtc tcgcatccgg caatcacctg cgccatcccc ggcaccggca acccgaagca
       61 catgaaggac aacctcggcg ccggactcgg gccactgccg gacgagaatg cccgccggcg
      121 aatggccgag tacttcgatg cccaaggttg agatccggcg cgagatcgtc gagcgcgtgc
      181 tggcgcgccg cggccggttg catttgttcg actcgctcga tccgaagcga agcgcgcttc
      241 tcgtcgttga catgcagaac gccttcgtcg cgcccggcgc gccgatcgag gtgcccgcgg
      301 cgcgcgggat cgtggcgccg atcaaccagc tcgccgccga gctccggcac cggggcgtga
      361 ccatcgtctg ggtggcgcac gagaaccgcg ccgacggccg cgactgggcg gggttcttcg
      421 acgccttcgt cgctcccggc cgccgcgccg aggccgccgc ggcgctcgcc ggcggctcga
      481 agctgcagca gctctggccc gagctcgacg tgaaccccgg tgacctgcgc gtcagcaaga
      541 accgctacag cccgctcatc ggaaattctt ctctggagaa attgctcaga gaaaaaggga
      601 tcgacacact gcttctcgcc ggaaccaaga ccaacgtttg cgtcgagtgc accgcgcgcg
      661 acgcgatgat gctcgacttc aaggtggtgc tgctctccga ctgcaccgca tcgctctccg
      721 acgacgagca gcgtgcgacg ctagaaaacg taatccagca gttcggcgat gtgcgcacag
      781 ccgaagaggc tctcgccctg tttacatgat cacgcatacc ggcggctgcc actgcggccg
      841 cgtcaggttc gaggtgcagg cgccggcgaa gctcgacgtg ctcgagtgca actgctccgt
      901 atgcagcaag gcgggctacc tgcacctcat cgtcccggct gatcgcttca aactactttc
      961 tgggaaggat atgctgaccg gctacggctt caacacccat acagcgaagc atttgttctg
     1021 ttcggtatgc ggcatcaagt cgttctacgt accgcgcgcc gaccccaaca gccgcagcgt
     1081 gaacgtgcgc tgcctcgatc cgggcacggt cgaagggatg acaatcacac agttcaacgg
     1141 ccgcgactgg ggaaggcgag gatattcgcc gtgatggggc taaccgcgcc ggtcgatgaa
     1201 gcgcgcggcc ttgacgttgg cttcgaattc cctagcgagc gtcccggttt cgagcaccac
     1261 gatctggggc gcgagctcga aggtatgctt gatcccctct gccagagcac gggataccgc
     1321 tgccgaatcg actcccttct tcgtctctat caaaaccctg aggatgtcgt tgccgccctc
     1381 gttcaccgcc tcggcgcgga agtcgccgat ctcgaggtgg cggaacatga agtcctcgag
     1441 atcctggtga cccaggttga cgccgcggat cttgaagaag ccggcggtgc ggtgcgcgtg
     1501 cttgatgcgc ggaaacaccg agaagggacc ttcgccgtca tcggcctcgc tgtaggtgac
     1561 gagatcgccg gagctccagc gcaggaacgg cgtgatgttg ttcgcccaga gcgccgtcac
     1621 gactagcgtg ccgatctcgc cttctctggc cggctcgagc gtcttgggat cgagcacttc
     1681 gatgtagtac atgtcggtcc agatgcggaa gcccgcgccc ggtttgtctt ccgcgcccat
     1741 cattccggcc tcggtcatgc cgaaggtgtc gatgagcttt gcgccccagc tacgctcgat
     1801 cttgacgcgc ttcgcctcgg ataccggctc ggcggaagtc atcagcttgt cgatgcgaag
     1861 ctcaagcccg cgcgcttcgg cgagattggc gaggtgcagc gcatagctcg acatgccgag
     1921 ccagatcgtc ggcttgaggc ggcggatgag ctcgatctgc agcgccgaag gcgtcgaggt
     1981 gccagcgccg gcccagttca cggtgacgcc gcgcgagccc gcgcagcgcg cgaaaatctg
     2041 acctaccggg tggatgccca tggggaacga cacgtgcgct cggtcgccag gcttcgcgcc
     2101 cgcgcagtcg aaggtacgcg cgaatgcctc gaggccgtat tcgatatcgc ggaaactgcg
     2161 cggatagaag agcggctggc cggtaacacc gccggagcgc cagtactcgg cgatgccgtc
     2221 gtggccggag acgcagaact cgccgtagaa ctccgcgtcg gtg
//
LOCUS       DASTPD010000039         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1038158, whole genome shotgun sequence.
ACCESSION   DASTPD010000039 DASTPD010000000
VERSION     DASTPD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038158"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1040
                     /locus_tag="VFR57_01690"
     CDS             30..1040
                     /locus_tag="VFR57_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258116.1"
                     /translation="MKRLILALALATPGFAFAQGNLVLYCTVQEEWCRAMVTAFERKT
                     GIKVAMTRKSSGELYAQVKAEAANPRGDMWWGGTGDPHLQAAEEGLTLEYRSPMLKDL
                     APWAVKQAEQSKYRTVGVYAGVLGFGYNSELLAKKKLPEPKCWSDLLSDRYKDEVQVA
                     DPNSSGTSYTMLATIVQLHGEDKGFDFMKRLHRNINQYTKSGAAPAKATASGETLIGI
                     TFMHDLVTFAVQGNPIKVVAPCEGTGYEVGSMSIIKGARNLDQAKAWYDWALTPEAQS
                     IGAEAKAFQIPSNKNAKVPPQAPRLDQTKVIDYDFAKYGSSAERKRLLSKWDKEVKAL
                     PK"
     gene            1007..>2457
                     /locus_tag="VFR57_01695"
     CDS             1007..>2457
                     /locus_tag="VFR57_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0258117.1"
                     /translation="MGQGSQGTTQVVWIAATAAGLALLPWYAVEGARWGGPALWHGFQ
                     GKPWLLPPLLPLIGAAVMRPWALGRRSAGVVLIVTGGVGLVLLLLQGFLFRHHNVVGA
                     LSAAQQALVVLRGFFFVPSPADVEPSKFPTPGMGYGALVAAAGYLMLFCQGLAHRGFC
                     RGDAFVVGAIGLVVASIAAFVFFPIFVMLESAFRDNAGKLAPSAFATSFFDRSVWGLD
                     CLTSSLRCGVAWNTVFLAVVVGAGSTFLGLAFALIAARTAFRFKGVLRVMSVLPIITP
                     PFVIGLAVILLFGRAGAVSTLLFDWFDIPRARWIYGLPGVALAQLLAFTPIAFLVLVG
                     VVQGISPSLEEASQTLRARGWTTFRTVTLPLLRPGLAAAFLLGFVESMADFGNPLVLA
                     GNFEVLSTKIYFAVVGASHDQSRAAVLALVLLVFTLAAFWAQQRWLGRASYTTVTGKG
                     DAGLPVPLPRGLRWLCYGIALPWAALTLVIYAT"
BASE COUNT          369 a          795 c          828 g          465 t
ORIGIN      
        1 gctaacgaaa acctgaagag gaggatgtca tgaagcgatt gatcctggcc ctggcgctgg
       61 ctacgccggg atttgccttt gctcaaggca acctggtgct gtactgcacg gtgcaggagg
      121 agtggtgccg cgccatggtc actgccttcg agaggaaaac cggtataaag gtcgcaatga
      181 cgcgcaagag tagcggcgag ctctatgcgc aggtcaaggc ggaggctgcc aatccgcgcg
      241 gcgacatgtg gtggggcgga accggcgatc cgcacctgca ggcggccgag gaggggctga
      301 cgctcgaata ccgctccccg atgctgaagg acctcgcgcc ttgggcggtg aagcaggccg
      361 aacagtcgaa gtaccgcacg gtcggcgtat acgccggcgt gctcggcttc ggctacaaca
      421 gcgagctgct ggcgaagaag aagctgcccg agccgaagtg ctggagcgac ctcctgtccg
      481 acaggtacaa ggacgaggtt caggtcgccg atccgaactc ttccggcacg tcgtacacga
      541 tgctcgcgac gatcgtgcag ctgcacggcg aggataaggg gttcgacttc atgaagcgcc
      601 tgcaccgcaa catcaaccag tacacgaagt cgggcgccgc cccggccaag gccaccgcga
      661 gcggcgaaac cctcatcggc atcaccttca tgcacgacct cgtgaccttc gcggtgcagg
      721 gcaacccaat caaggtggtc gcaccctgcg agggcaccgg ctacgaggtc ggctccatga
      781 gcatcatcaa gggggcgcgg aacctcgacc aggcgaaagc gtggtacgac tgggcgctca
      841 cgccggaagc gcagagcatt ggcgccgaag caaaggcgtt tcagatcccg tcgaacaaga
      901 acgccaaggt cccgccgcag gcgccacggc tcgaccagac caaggtgatc gactacgact
      961 tcgcgaagta cggctcgtcg gcggaacgta agcgcctcct gtcgaagtgg gacaaggaag
     1021 tcaaggcact acccaagtag tctggatcgc cgccacggca gcaggcctcg cgctgctgcc
     1081 gtggtacgcg gtcgaaggcg cgcgctgggg cgggccggcg ttgtggcatg gcttccaggg
     1141 gaagccgtgg ctgctaccgc ctctcttgcc gctgatcggc gctgcagtga tgcggccttg
     1201 ggcgttgggg cgccgcagcg cgggtgtcgt cttgattgtc acgggcggtg tcgggttagt
     1261 tctgttgctc ctgcaagggt ttctgttccg ccatcacaac gtcgtcggtg cgttgtcagc
     1321 ggcacagcaa gcgctggtcg tcttgcgggg atttttcttt gtcccttcgc ctgccgacgt
     1381 tgagccgtcg aagttcccga cgccgggaat gggatacggt gccttggtcg ccgcggccgg
     1441 ctatctgatg cttttctgcc aggggttggc gcatcgcggg ttttgccgcg gcgatgcctt
     1501 cgtcgtcggc gcgattgggc tggtggtggc atcgatcgcc gcgttcgtat tcttcccgat
     1561 cttcgtgatg ctcgagagcg cgttccgcga caacgccggc aagctcgccc cctcggcgtt
     1621 cgccacgagc tttttcgacc gctcggtgtg gggcctcgac tgcctgacct ccagcctgcg
     1681 ctgcggcgtg gcgtggaaca cggtgtttct cgcggtggtg gttggcgcgg gctcgacgtt
     1741 cctcggcctc gcgttcgcgc tgatcgccgc gcgcaccgcg ttccgcttca agggcgtgct
     1801 gcgcgtgatg agcgtgctgc cgatcatcac gccgccgttc gtcatcggcc tcgccgtgat
     1861 cctgctcttc ggccgcgccg gcgcggtgtc gacgcttctc ttcgactggt tcgacatccc
     1921 gcgcgcgcgc tggatctacg gccttccagg cgtcgcgctc gcgcagctcc tcgccttcac
     1981 gccgatcgcg ttcttggtgc tcgtcggcgt ggtgcagggc atcagtccct cgctcgagga
     2041 ggcctcgcag accctgcgcg cgcgcggctg gaccacgttc cgcacggtca ccctgccact
     2101 tctgcgtccc gggctcgccg ccgcgttcct gctcggcttc gtggagagca tggccgactt
     2161 cggcaatccc ctcgtgctcg cgggcaactt cgaggtgctc tcgaccaaaa tctatttcgc
     2221 ggtggtcggc gcctcgcacg accagagccg tgcggcggtg cttgcgctcg tccttctcgt
     2281 ctttacgctg gcggcgttct gggcgcagca gcgctggctc gggcgcgcgt cctacaccac
     2341 cgtgaccggc aagggggacg ccggcctgcc ggtgccgctg ccgaggggct tgcgctggct
     2401 ctgctacggc atcgcgcttc cctgggcggc cctcacgctg gtcatttacg cgaccat
//
LOCUS       DASTPD010000040         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1038457, whole genome shotgun sequence.
ACCESSION   DASTPD010000040 DASTPD010000000
VERSION     DASTPD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038457"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFR57_01700"
     CDS             <1..312
                     /locus_tag="VFR57_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEU0258118.1"
                     /translation="AIGAFSEAMLGYFDWEKKDYERIPVREQTEVLALVGDITLDDAG
                     GRKVHAHVVLGKRDGAACGGHLLAAKVRPTLELILTESPGYLRRRHDPASGLALIRTR
                     D"
     gene            449..772
                     /locus_tag="VFR57_01705"
     CDS             449..772
                     /locus_tag="VFR57_01705"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0258119.1"
                     /translation="MAHYMLQASFTSEAWAKMLNEPQNREQAIRPVIEKLGGKLLGYW
                     FAFGESDAVVIVQMPDNVSAAAASLAAAAGGAVKSVKTTPLMSVEEGMQAMRKAGGAG
                     YRAPG"
     gene            805..2070
                     /locus_tag="VFR57_01710"
     CDS             805..2070
                     /locus_tag="VFR57_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258120.1"
                     /translation="MLRALAPEVRVREVWAWSMYDFANSAYTTVVITAVFSAYFVGAV
                     AENKPWATFAWTAALSVSYAAILFTGPLIGAWADAHAAKKRLLFATTLGCVLFTAALY
                     FAQPGAIALALVFLIFSNYFFGAGENLIAAFLPELADSRAMGRVSGWGWSFGYLGGLT
                     ALGICLYYITLSSSAGKPASHFVPVTMLITAAFFAAAAVPTFLFLRERAVPQPRMENP
                     WVRVKETLQHAQQYQDLRRFLLCLLFYQAGITAVVSLAAIYAEQAMKFTTQQTITLIL
                     VVNVTAAVGAFGFGYLQDAVGHVRAVAITLLGWIVMVVIAGFSQSHSSFWLAANLAGL
                     CMGSSQAAGRALVGYLAPPARLAEFFGLWGLAVKAASIFGPLTYGAVTLVFAGNHRLG
                     IFVTGLYFVIGLFLLRRIDVERGRVAALA"
     gene            2192..>3087
                     /locus_tag="VFR57_01715"
     CDS             2192..>3087
                     /locus_tag="VFR57_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0258121.1"
                     /translation="MKQQGPAVVNVITKREAARSGRGGAQGQPPEDPLLEFFRRFMPD
                     APERMPGPGLGLGSGFIVSQDGYVLTNAHVVAGDGEVTVRLADAKREFKARVIGADER
                     TDIALLKIEATGLPTVKLGKSEKLQPGEWVAAIGSPFGFENTITAGIVSATGRSLPAE
                     TYVPFIQTDVAVNPGNSGGPLMNLAGEVVGINSMIYSQTGGYMGVSFAIPIEVALDVM
                     KQLRAEGKVTRGRLGVRIQPMTKELAQSFRLKEPNGALIASVEPGSPADKAGLQPGDV
                     VLAYNGQPLEDANKLPRLVAAT"
BASE COUNT          486 a         1027 c         1022 g          552 t
ORIGIN      
        1 gccattggcg ccttcagcga ggcaatgctc ggctatttcg actgggaaaa aaaggactac
       61 gagcgcatcc cggtgcgcga gcagaccgag gtcctcgctc tggtcgggga catcacgctc
      121 gacgacgctg gcggcaggaa agtgcacgcg cacgtggtgc tgggcaagcg tgacggcgcc
      181 gcgtgcggcg gccacctgct ggccgcgaag gtgcgcccga ccctggagct catcctcacc
      241 gagtcgcccg gctacctcag gcgccggcac gatccggcca gcgggctcgc gcttatccga
      301 acgcgcgatt agtcccgaaa tagattccct caaccgggcg caatgacggg tctcgggttg
      361 agcgagcgcg acgcgcgcga tatcgccgcc tacctcgcca gtttgaaata gactgccatt
      421 ccccattccg gggacagagg aggcagtcat ggcgcattac atgttgcagg cgtcctttac
      481 gtccgaagcg tgggcgaaga tgctcaacga gccgcagaac cgggagcagg cgatccggcc
      541 ggtgatcgag aagctcggcg gcaagctcct cggctactgg tttgcgttcg gcgaatcgga
      601 tgcagtcgtc atcgtgcaga tgcccgacaa cgtgtcggcg gcggccgcgt cgctcgctgc
      661 agctgccggc ggcgcggtca agtcggtgaa gacaacgccg ctcatgtcgg tcgaagaggg
      721 catgcaggcg atgcgcaagg cgggcggggc cggctaccgc gcgcccggct gagcagccgc
      781 ggcttcctgg ccgttccgtt cgcgatgctc cgcgccctgg cccccgaagt gcgtgtgcgc
      841 gaggtctggg cgtggagcat gtacgacttc gcgaactcgg cctacaccac ggtcgtcatc
      901 accgcggtgt tcagcgccta tttcgtcggg gccgtagccg agaacaagcc ctgggcgaca
      961 ttcgcctgga ccgccgcgct ctcggtgtcc tacgcggcga ttctcttcac cgggccgctc
     1021 atcggcgcgt gggccgatgc gcatgcggcg aagaagcggc tgctcttcgc cacgaccctc
     1081 ggctgcgtgc tcttcaccgc ggctctctac ttcgcgcagc ccggtgcgat cgcgctcgcg
     1141 ttggttttcc tgattttttc caattatttc ttcggtgcgg gcgagaacct catcgccgcc
     1201 ttcctgcccg agctcgcaga ctcgcgcgcg atgggccgag tctcaggctg ggggtggagc
     1261 ttcggctacc tcggcggcct gaccgcgctt ggcatctgcc tgtactacat caccctcagc
     1321 agctcggcgg ggaaacccgc ctcgcacttc gtgccggtga cgatgctgat taccgccgca
     1381 tttttcgcgg ctgccgcggt gccgacgttt ctttttctgc gcgagcgcgc cgtcccgcag
     1441 ccgcgcatgg agaacccgtg ggtgcgggta aaggagacct tgcagcacgc gcagcagtac
     1501 caggacctcc ggcgctttct gctctgcctg cttttctacc aggcgggaat caccgcggtg
     1561 gtctcgctcg cggcgatcta cgccgagcag gcgatgaagt tcaccaccca gcagaccatc
     1621 accctcattc tcgtcgtcaa cgtcaccgcg gcggtcggtg cattcggctt cggctacctg
     1681 caggacgcgg tcggccacgt gcgcgcagtg gcgattacgc tcctgggatg gatcgtgatg
     1741 gtcgtcatag cgggtttttc gcaaagccat tcttcattct ggcttgccgc gaaccttgcc
     1801 gggctctgca tgggatcgtc ccaggcagcg ggccgggcac tcgtcggcta ccttgcgccg
     1861 ccggcgcgcc tggcggagtt cttcgggctt tgggggctgg cagtgaaggc ggcttcaatt
     1921 ttcggcccac tcacctatgg cgcggtgacg cttgtcttcg ccggcaacca ccgcctcggc
     1981 atcttcgtca ccgggctcta tttcgtgatc gggctgtttc tgctgcgcag gatcgacgtc
     2041 gagcgcggcc gcgtggcggc gctcgcgtag gcgcgaagct tgcagagcgg gtctcaccat
     2101 gagaccgctc gccctcatag tcttattgac tgcctttacg actgcggccc cgggtcagac
     2161 cgcccggaac ctgcccgact ttacctcgct catgaagcag cagggccccg cggtggtgaa
     2221 cgtgattaca aagcgcgagg cagcgcgcag cgggcgcggc ggagcgcagg gacagccgcc
     2281 ggaggacccg ctgctcgaat tcttccggcg cttcatgccc gacgctccgg agcgcatgcc
     2341 gggcccgggg ctcggccttg gctcgggctt catcgtcagc caggacggct acgtgcttac
     2401 caacgcccac gtagttgccg gcgacggcga agtcaccgtg cgcctcgccg atgcgaagcg
     2461 tgagttcaaa gcgagggtga tcggtgcgga cgagcgcacc gacatcgcgc ttctcaagat
     2521 cgaagccacc gggctgccca ccgtgaagct cggcaaatcg gagaagctgc agcccggcga
     2581 gtgggtggcg gcgatcggct ctccgttcgg cttcgagaac acgatcaccg ccgggatcgt
     2641 gagcgccacc gggcgctcgc tgcccgcgga gacctacgtc cccttcatcc agaccgacgt
     2701 cgccgtgaac cccggcaact ccggcgggcc gctcatgaac cttgcgggtg aggtggtcgg
     2761 catcaattcg atgatctaca gccagaccgg cggctacatg ggcgtgtcgt tcgccattcc
     2821 gatcgaggtt gcgctcgacg tgatgaaaca actgcgtgcc gagggcaagg tgacgcgcgg
     2881 ccggctcggc gtgcgcatcc agccgatgac caaggagctc gcgcagtcgt tccggctcaa
     2941 ggagccgaat ggcgcgctga tcgcgtcggt cgagccagga agcccggccg acaaagcggg
     3001 cctccagccc ggcgacgtgg tactggcgta caacggccaa ccgctcgagg atgccaacaa
     3061 gctgccgcgc ctggtcgccg cgaccaa
//
LOCUS       DASTPD010000041         5247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_835573, whole genome shotgun sequence.
ACCESSION   DASTPD010000041 DASTPD010000000
VERSION     DASTPD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835573"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFR57_01720"
     CDS             <1..323
                     /locus_tag="VFR57_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258122.1"
                     /translation="CLAGRRFGRARLGRWMMGACRSLWEERAARWTRGAPLGVVAGTL
                     PMGLGRAFGALPGPNDGVVCVEETSVDGMAGRALVRQGHSMLIVSGVVGNLVEGFLAC
                     GRFA"
     gene            320..1321
                     /locus_tag="VFR57_01725"
     CDS             320..1321
                     /locus_tag="VFR57_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU0258123.1"
                     /translation="MRVWVAFLLLGVVAGCQSLAYYTQAIGGHFKVLASARPVSDWLA
                     DPATPPELKARLETARRIREFASKSLGLPDNGSYLSYADIERPYVVWNVFAAPELSVE
                     PKVECFPFAGCVSYRGFYSEDEARRHADKLRADGYDVHVGGVPAYSTLGWSDDPLLST
                     FIRYSDAQLARLVFHELAHQVVYARNDTTFNESFAVTVEEEGVRRWLQSENRSGELAA
                     FSSVQARKRDLAERVSQARERLKGIYTSDLAREQKLAQKSVEWQRLRSAYPALPIEPN
                     NAFLASVAVYTELVPAFERLLAESGSLEAFYRRVKDLARDPSAREAYFTTAGYRRSS"
     gene            complement(1290..1907)
                     /locus_tag="VFR57_01730"
     CDS             complement(1290..1907)
                     /locus_tag="VFR57_01730"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0258124.1"
                     /translation="MLKIWGRVNSVNVKKVLWAAEELGLRYERIDAGREFGVVNTPQY
                     KKMNPNSLVPTIDDSGFVLWESHAIVRYLAAKHGAGTLWPSDLRQRADSDRWMDWAFT
                     FQASLRAVFWTLVRTPPEKRNMQAVEEGRKKCAELLAIPDEALANRLYFAGNVLTIGD
                     IPLGCHVQIWMRLPIERPAHPNLSRWFDRLCARAAFKKIVDIPLS"
     gene            complement(2026..3612)
                     /locus_tag="VFR57_01735"
     CDS             complement(2026..3612)
                     /locus_tag="VFR57_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU0258125.1"
                     /translation="MTAFAWNNPYPWPRKPLLAANVVATSQPLAAQAGLQMLAGGGSA
                     VDAILATAITLTLVEPVSNGIGSDAYAIVWDGERLHGLNASGRSPAGWTPEFFAGRSS
                     MPERGWNSVSVPGCVSAWVELHRRWGKLPFEKLFEKAIEYGRKGFLVSPTIAGQWEKQ
                     VPELKPQPGFADAFMPDGGPPRPGQLFRFAKHAETLERIRDTKGEAFYRGELAEKLEA
                     HAKKHGAAMRASDLAAQKSDWVEPLSVDYRGYTLHEIPPNGQGIVALMALGMLEHFDL
                     RSHPVDGADSVHLQIEAQKLAFADARRYVADIDYMTSVRPQALLDKEYLNARAKLIEL
                     KRAQDFGHGAPPQSGTVYLAAADASGLMVSFIQSNFMGFGSGVVVEGISLQNRGATFV
                     LQPGHPNCVGPRKRPYQTIIPGFVTRAGRPVMSFGVMGGTMQPQGHAQVMVRIADYGQ
                     SPQAACDGPRFRVVQGMDVSVESAFRPSVLEELKRRGHRIVSLPDDYNQFGSAQLIWK
                     LDGGYFTASDPRRDGQAVGF"
     gene            complement(3626..4681)
                     /locus_tag="VFR57_01740"
     CDS             complement(3626..4681)
                     /locus_tag="VFR57_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEU0258126.1"
                     /translation="MLSWQIIEHGKPLQRILSDTPKPQGSEVLVRITRAGVCHSDVHI
                     WEGYFDLGGGKRFYVKERGCVPPFILGHEPFGVIEALGPRARGVRVGQKKIVYPWIGC
                     GKCAVCRAGQDNYCVSGIRFLGVSRPGAYSTHLLVPDAKYLVDPAGIDEAFAATLACS
                     GLTAYSAAAKLPQLWPQERVAVLGCGGLGLIGISVLRAKGVKHVVACDIDDAKLAAAA
                     RLGAKTTLNTKNPDATRELLGIAAAIDFVGSPVTAALGIGALRKGGRYIICGLYGGEL
                     VHPLPPIAQRAIGIVGSYVGSLQELKEVVSLAKRRKIRPAPVELRPADEAARALDDLK
                     AGRVLGRIVLDFENVAA"
     gene            complement(4692..>5247)
                     /locus_tag="VFR57_01745"
     CDS             complement(4692..>5247)
                     /locus_tag="VFR57_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0258127.1"
                     /translation="HGVIFHETFGDFGYTMKARMEIMRTFGPALSPMNAWLLLQGLES
                     LHVRMDRHCANALAVAKFLQGHPRVAWVNYPGLADSKYHSLAKKYLPKGASGLLNFGV
                     KGGARAGERFIEAAQFMSHLANIGDAKTLVIHPASTTHRQMDEADQLKAGVTPDMIRI
                     SVGIETLDDILWDIDQALAEAAKV"
BASE COUNT          847 a         1753 c         1717 g          930 t
ORIGIN      
        1 gctgcctcgc cggccggagg ttcgggcgcg cgcggctcgg gcgctggatg atgggcgcat
       61 gccgctcgct gtgggaggag cgcgccgcgc gctggacgcg cggcgcaccc ctgggcgttg
      121 tcgccggcac gcttcccatg gggctagggc gcgcattcgg cgcgttgccg ggaccgaacg
      181 acggcgtggt gtgcgtcgaa gagacttccg tcgacggcat ggccggccgg gcactggttc
      241 gccagggcca cagcatgctt atcgtgtcgg gggtggtcgg caacctggtc gagggcttcc
      301 tcgcgtgcgg acggttcgca tgagagtctg ggttgcattc ctgttgctcg gcgttgtcgc
      361 gggatgccag tcgctcgcct attacacgca agcgatcggc ggtcacttca aggtgctggc
      421 gagcgcgcgg ccggtctccg actggcttgc cgatccggcg accccgccgg agctcaaggc
      481 gcgcctcgag actgcgcgcc gcatccgcga gttcgcctcg aagagcctcg ggctgccgga
      541 caacggcagc tatctttcct acgccgacat cgaacgcccc tacgtggtgt ggaacgtatt
      601 cgcggcgccc gagctctcgg tggagccgaa ggtcgaatgc ttccccttcg ccggctgcgt
      661 gagctaccgc ggcttctact ccgaggacga agcgcggcgc catgccgaca agctacgcgc
      721 ggacggctac gacgtgcacg tcggcggcgt gccggcgtac tccacactcg gctggtccga
      781 cgatccgctg ctctcgacct tcatccgtta ctccgatgct cagcttgcgc ggctcgtatt
      841 ccatgagctc gcgcaccagg tggtctacgc cagaaacgac accactttca acgagtcctt
      901 cgccgtgacg gtggaggagg aaggcgtgcg gcgttggctc cagtcggaaa accgttccgg
      961 cgagctggcg gcgttcagtt ccgtccaggc gcgtaagcgt gatctcgccg agcgcgtgag
     1021 ccaggcgcgc gagcgcctga aaggcatata cacctcggat ctcgcgcgcg agcagaagct
     1081 cgcgcagaag agcgtcgagt ggcagcggct gcggtcagcg tatcccgcgc tgcccatcga
     1141 gccgaacaac gccttccttg cctcggtggc ggtctacacg gagctcgtgc cggcgttcga
     1201 gcgcctgctc gcggagagcg ggagcctgga ggcgttctac cgtcgggtga aggacctggc
     1261 gcgcgatcca tccgcacgcg aggcctactt cacgacagcg ggatatcgac gatcttcttg
     1321 aaagccgcac gcgcgcacag ccggtcgaac caccggctca ggttcgggtg cgccggacgc
     1381 tcgattggca gtcgcatcca gatctgcacg tggcagccaa gcgggatgtc gccgatcgta
     1441 agcacgttgc cggcaaaata aagccggttc gcgagcgcct cgtccgggat ggcgagcagc
     1501 tcggcgcatt tcttacgccc ttcctcgacc gcctgcatgt tgcgtttctc cggcggcgtg
     1561 cgcacgaggg tccagaacac cgcgcgcagc gacgcctgga aggtgaacgc ccagtccatc
     1621 cagcggtcgg agtcggcgcg ctgcctgaga tccgacggcc acagcgtgcc cgcgccgtgc
     1681 ttcgccgcga ggtagcgtac gatcgcgtgc gactcccaga gcacgaagcc gctgtcgtcg
     1741 atcgtcggca caagcgagtt ggggttcatc ttcttgtact gcggcgtgtt cactacgccg
     1801 aactctctgc cggcatctat gcgctcgtac ctgaggccga gttcctccgc ggcccacaac
     1861 acctttttta cgttaactga attgactcgt ccccagattt tcagcattgg gtctccgaaa
     1921 tcggggtcag agcccgattg atttccattg gagttaagaa atacaccgaa acgcaagacg
     1981 tgccttcgcc catcgtcatc gtaatcgggc tctgaccccg attgtctaga aacccaccgc
     2041 ctgcccgtct ctccgggggt cgctcgcggt gaagtagccg ccgtcgagct tccagatgag
     2101 ctgagcgctg ccaaactggt tgtagtcgtc cggaagcgaa acgatgcggt ggccgcgccg
     2161 cttgagctct tccagcaccg aaggccggaa ggcactttcc acgctcacgt ccatgccctg
     2221 cacgacgcgg aagcgcggcc cgtcgcacgc cgcttgcgga ctctggccgt agtcggcgat
     2281 gcgcaccatc acttgggcat ggccctgcgg ctgcatggtg ccgcccatga cgccgaagct
     2341 catgacgggc cggcctgcgc gcgttacgaa gcccggaatg atcgtctggt acggccgctt
     2401 gcgcggcccg acgcagttcg ggtggcccgg ttgcagcacg aaggtcgcgc cccggttctg
     2461 cagcgagata ccctccacca ccacgcccga gccgaagccc atgaagttcg actggatgaa
     2521 ggacaccatc agcccggagg cgtcggctgc cgcgaggtat accgtgccgc tctgcggcgg
     2581 cgcgccgtgg ccgaaatcct gcgcgcgctt aagctcgatc agcttcgctc tcgcgttcag
     2641 atactccttg tcgagcagcg cctgcggccg cacgctggtc atgtagtcga tgtcggcgac
     2701 gtagcgccgc gcgtcggcga acgccagctt ctgcgcctcg atctgcaagt gtacgctgtc
     2761 ggcgccgtcc accgggtgcg agcgcaagtc gaagtgctcg agcatgccga gcgccatgag
     2821 cgcgacgatg ccctggccat tcggcgggat ctcgtgcagc gtgtagccgc ggtagtccac
     2881 cgaaagcggc tcgacccagt cggacttttg cgcggcgaga tcggaagcgc gcatcgcggc
     2941 gccatgtttt ttcgcgtgcg cctccagttt ctccgccagc tcgccgcggt aaaacgcttc
     3001 gcccttggta tcgcgaatgc gttccagcgt ctcggcgtgt ttggcgaacc ggaaaagctg
     3061 ccccggtcgg ggagggccac cgtccggcat gaaggcgtcg gcgaagccgg gctgcggttt
     3121 gagctcggga acttgcttct cccactgccc ggcgatagtc ggcgagacga ggaagccctt
     3181 gcgcccgtac tcgatcgcct tttcaaagag cttttcgaac ggtagcttgc cccaccggcg
     3241 gtgcagttct acccaggcgg agacgcaccc cggcacgctg accgagttcc agccgcgctc
     3301 gggcatgctc gaacgaccgg cgaagaactc cggcgtccac ccggccggcg agcggcccga
     3361 ggcgttcagc ccgtgaagac gctctccgtc ccagacgatc gcataggcgt ccgagccgat
     3421 tccgttcgat actggctcga cgagcgtgag cgtgatcgcg gtggccagga tcgcgtccac
     3481 agcgctgcct ccaccggcga gcatttgcag cccggcctgc gccgcgagcg gctgcgaggt
     3541 ggcgacgacg ttcgccgcca gcagcggctt gcgcggccag ggataggggt tgttccaggc
     3601 gaaagccgtc attggggatt ggtgttcacg ccgccacgtt ttcgaagtca agcacaatcc
     3661 gccccagcac ccggccggcc ttcaggtcgt ccagcgcgcg cgccgcttcg tccgcggggc
     3721 gcagctccac cggcgcggga cgtatcttgc gccgcttggc gagcgagacc acttccttca
     3781 gctcctgcaa gcttccgacg tacgagccca cgatgccgat ggcacgctgg gcgatgggcg
     3841 gcaaaggatg gacgagctcg ccgccgtaca ggccgcaaat gatgtagcgt ccgcctttgc
     3901 gcaacgcgcc gatgccgagc gccgcggtca ccgggctgcc gacgaaatcg atcgcggcgg
     3961 cgatgcccag caactcgcgc gtggcatccg ggtttttcgt gttcaaagtc gtcttggccc
     4021 caagcctggc tgcggccgcg agcttcgcgt cgtcgatatc gcaagcgacc acgtgtttca
     4081 cgcccttggc gcgcagcacc gatatgccga taaggccgag gccgccgcag ccgagcaccg
     4141 cgacccgctc ctgcggccag agttgcggca gcttggcggc cgcgctgtac gcggtgagcc
     4201 cggaacaagc gagggttgcg gcgaaggcct cgtcgatccc cgctgggtcg acgaggtact
     4261 tggcgtccgg cacgagaaga tgggtcgaat acgcgcccgg gcggctgacg ccaagaaagc
     4321 ggatccccga gacgcagtag ttgtcctggc cggctcggca taccgcgcat ttcccgcagc
     4381 cgatccacgg gtacacgatc tttttttgtc ccacccggac gccacgggcg cgggggccca
     4441 acgcctcgat cacgccgaaa ggctcgtggc cgagaatgaa aggcggcacg caaccgcgtt
     4501 ccttcacgta gaagcgcttg ccgccgccaa ggtcgaagta cccctcccag atgtgcacgt
     4561 cggagtggca aacccccgcc cgcgtgatgc gaaccagcac ctcgcttccc tgcggtttcg
     4621 gagtgtccga caagattctc tgcagaggct tcccgtgctc gatgatctgc caactcagca
     4681 tctttcctcc cttacacctt ggcggcctcg gcgagcgcct ggtcgatgtc ccagaggatg
     4741 tcatccaggg tctcgatgcc cactgatatg cggatcatgt ccggtgtcac gccggctttg
     4801 agctgatcgg cctcgtccat ctgccggtgg gtggtcgacg cggggtggat cacgagcgtc
     4861 ttggcatcgc cgatgttggc caggtggctc atgaactgag ccgcctcgat gaagcgctcg
     4921 cccgccctcg cgcctccctt caccccgaaa ttgagcaggc cggacgcgcc tttcggcaga
     4981 tacttctttg ccagtgagtg gtacttgcta tctgcaagcc cgggatagtt cacccaggcg
     5041 acccgcggat gaccctggag aaacttggct acggcgagtg cattcgcgca gtggcggtcc
     5101 atccggacgt gcagcgactc cagcccctgc agcagcagcc aggcgttcat cggcgagagc
     5161 gccgggccga aggtgcgcat gatctccatg cgcgccttca tggtgtagcc gaagtcgccg
     5221 aaggtctcgt ggaagatgac gccgtgg
//
LOCUS       DASTPD010000042         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1448128, whole genome shotgun sequence.
ACCESSION   DASTPD010000042 DASTPD010000000
VERSION     DASTPD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448128"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFR57_01750"
     CDS             complement(<1..685)
                     /locus_tag="VFR57_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0258128.1"
                     /translation="MERFKAYKLTETPEKKVRAEFVDYTLDQLDPHEVLVRVAYSSVN
                     YKDALAATGKGKILRRPACVGGIDFAGTVIESRDPRFKKGDAVLTASGEYVGVSSDGG
                     YAPYTRVKGDWIAKVPPGLSLWEAMAFGTAGYTAGIAIVRMEHNGLKPENGPVIVNGA
                     TGGVGSIALAALAKLGYHVVALTGKESEADWLKKLGAKEIMLRQTLNLQKIKPLDKAT
                     WAGAVDNLGG"
     gene            complement(700..1650)
                     /locus_tag="VFR57_01755"
     CDS             complement(700..1650)
                     /locus_tag="VFR57_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258129.1"
                     /translation="MRTLVALVLAVACGLGFAQSYPVKPVRLIIPVPPGGSNDVVGRS
                     IGMQLGERLGQSVVIDNRGGAGSIIGTNEAAKAAPDGYTLLLISTAFPTSIAFNRLPR
                     DAMKSFVPVAMLGSGPALLVVPASSAANSVTDLLNLLKQKPGELNAAAAGIGSFQHMA
                     TELFRLQSKTNFVIVQYKGGGPALTDVIAGQVQMNLGSLVQMIPHVRSGKLKALGVSS
                     AKRVAALPDVPTIAEAGVPGFEVSNWWGILAPAGTPEPALDRLHKEITAILDSPETRK
                     RFELEGAEVVRMKPQEFAAFVTQETEKWTRVVKEAGIKPE"
     gene            complement(1647..2738)
                     /gene="zapE"
                     /locus_tag="VFR57_01760"
     CDS             complement(1647..2738)
                     /gene="zapE"
                     /locus_tag="VFR57_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEU0258130.1"
                     /translation="MSEAQGVVAVYAQSLARRGFVSDSAQWRAVERLQRLHEEWGAYK
                     RRRSSALRRLLVKPPLPKGVYLWGPVGRGKSFLMDAFYLCVPLVRKRRVHFHHFIREI
                     HRELDALKGAQDPIAEVAARTARRYRLVCFDEFHVSDIADAMILGRFLDQVMDRGVQF
                     VMTSNYHPDQLYPGGLQRERFVPAIELIKSRLDVVAVDSGTDYRRLKMEQVQVYHLSD
                     APLARIFEELKDVQEEWHPLDVEGRTIPYRKRAGGVVWFDFHTLCGGPRSYADYVDLA
                     KRFHTVVLSGVPRMSARQSDAARRFTWLVDVLYDERVKLVVSAEAAPEELFVEGQNSA
                     DFQRTVSRLHEMQSAEYRLLPARRVALAA"
     gene            complement(2731..>3434)
                     /locus_tag="VFR57_01765"
     CDS             complement(2731..>3434)
                     /locus_tag="VFR57_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0258131.1"
                     /translation="ELGAKITKVETNKTVKVQYTVSGNSSQTLECDRLIVCIGRVPNT
                     TGLNAAGVGLKLDERGFIEVDDECRTNLPNVWAIGDVVRGPMLAHKAEEEGVAVAERI
                     AGQKPHFDFNTVPWVIYTAPEIAWVGKTEQQLKAERVEYRAGVFPFSANARARALGDT
                     SGLVKVLAASKTDEILGVHIIGPFASELIAEAVVAMEFRGSAEDIGMIIHAHPSLSEA
                     LKDAALGVDKRSLNI"
BASE COUNT          585 a         1157 c         1058 g          634 t
ORIGIN      
        1 cgccgccgag gttgtccacc gcccccgccc aagttgcctt gtcgagaggt ttgattttct
       61 gcaggttcag ggtttgccgg agcatgatct ccttcgcgcc cagcttcttc agccaatccg
      121 cttcgctctc cttcccggtc aacgcgacca cgtgatagcc cagcttggcg agcgccgcta
      181 gggcaatcga gccgacgcca ccggttgcac cgttgacgat caccggaccg ttctcgggct
      241 tgagcccgtt gtgctccatg cggacgatcg cgatgcccgc ggtgtaaccg gcagtgccga
      301 acgccatcgc ttcccacagc gacaagccgg gcggcacttt ggcgatccag tcgcccttta
      361 cgcgcgtgta gggtgcgtag cctccgtcgc tcgacacacc gacgtattcg ccgctcgccg
      421 taagaacggc gtcgcccttc ttgaagcgcg gatctctgga ctcgatcacc gtgccggcga
      481 agtcgatgcc gcccacgcac gctggccttc tcaatatctt gcctttgccc gtcgccgcaa
      541 gcgcatcctt gtagttcacg ctcgagtacg cgacccgcac cagcacttcg tgcgggtcga
      601 gctggtcgag cgtgtagtcg acgaactcgg cgcgaacctt cttctccggc gtttcggtga
      661 gcttgtaggc tttaaagcgt tccatgtcgc ctcttcctat tattccggtt tgatgccagc
      721 ttccttgacc acgcgggtcc acttttcggt ttcctgcgtg acaaaagccg cgaactcctg
      781 cggtttcatg cgcacgacct cggcgccctc gagctcgaag cgcttgcgcg tctcgggtga
      841 atcgaggatc gcggttatct ccttatggag ccggtcgagc gcaggctccg gcgtgcccgc
      901 aggcgcgaga atgccccacc agttgctgac ctcgaaccca ggcacgccgg cctcggcgat
      961 ggtcgggaca tcgggcagcg cggcgacgcg cttcgcgctg ctgacaccga gcgccttcag
     1021 cttccccgag cgcacgtgcg ggatcatctg cacgaggctg ccgagattca tctgcacctg
     1081 gccggcgatc acgtcggtga gcgccgggcc gccgcccttg tactgcacga tcacgaaatt
     1141 ggtcttggac tgaaggcgga aaagctcggt cgccatgtgc tggaagctgc cgatgccggc
     1201 ggcggccgca ttgagctccc cgggtttctg tttcaacagg ttcagcagat cagtcacgga
     1261 gttcgcggct gaggatgccg gcacgacaag cagcgcgggc ccgctgccga gcatcgccac
     1321 cggaacgaag gacttcatcg cgtcccgcgg caggcggttg aatgcgatgc tcgtcggaaa
     1381 cgcggtcgaa atgaggagca gcgtgtaccc gtcgggcgcc gccttcgccg cctcgttcgt
     1441 gccgatgatg ctgcccgcgc cgccgcgatt gtcgatgacc acactctggc cgagccgctc
     1501 gccgagctgc atgccgatgg agcggccgac gacgtcattc gagccgcccg gcggaaccgg
     1561 gatgatgaga cgcacgggct tcacgggata ggactgggcg aacccgagcc cgcaagcaac
     1621 ggcaagcacc agtgcgacca gggttctcat gccgccaatg ctacccgcct tgccggcagc
     1681 agtcggtatt cggccgactg catttcatgc aaccggctca cggtgcgctg gaaatccgcc
     1741 gagttctggc cttcgacgaa aagctcctcg ggcgcagcct cagccgacac tacaagcttg
     1801 acgcgctcgt cgtacagcac gtcgacgagc caggtgaagc gccgcgcggc gtccgactgt
     1861 ctcgccgaca tgcgcggcac gccggagagc acgacggtgt ggaagcgctt cgccaggtcc
     1921 acgtaatcgg cataggagcg cggcccgccg cacagcgtgt ggaaatcgaa ccagaccaca
     1981 ccgcctgcgc gcttgcggta gggaatggtg cgtccctcga cgtcgagcgg atgccattcc
     2041 tcctgcacgt ccttcaattc ctcgaaaatc cgcgccagcg gcgcatcgct cagatgatag
     2101 acctgcacct gctccatctt gaggcgccgg tagtcggtgc cgctgtccac ggccaccacg
     2161 tccaggcggc tcttgatcag ctcgatcgcc ggcacgaagc gctcgcgctg caggcccccg
     2221 ggatagagct ggtcggggtg gtagttcgaa gtcatgacga attgcacgcc ccggtccatg
     2281 acctgatcca ggaagcgtcc gaggatcatg gcgtcggcga tgtcggaaac gtgaaattca
     2341 tcgaagcaca ccaatctata ccgccttgcc gtgcgcgccg ctacctcggc gatcgggtcc
     2401 tgggcgcctt tcagcgcgtc cagctcgcgg tggatctcgc gaatgaagtg gtggaaatgc
     2461 acgcgccgct tgcgcacaag cggcacgcac aaatagaacg catccatgag gaagctcttg
     2521 ccgcgcccga ccgggcccca cagatacacg cccttcggca gcggcggctt aacgagcagg
     2581 cgcctcagtg cgctggagcg gcgccgcttg tacgcgcccc attcctcgtg caggcgctgc
     2641 agccgctcga ccgcgcgcca ctgcgccgag tcggagacga agccgcgccg agcgaggctc
     2701 tgcgcgtaga ctgcgacgac gccctgcgcc tcagatattg aggctgcgtt tgtcgacccc
     2761 aagcgcggcg tccttcagcg cctcggaaag cgacggatgg gcgtggatga tcatgccaat
     2821 gtcctcggcc gagccgcgga attccatcgc caccaccgcc tcggcgatca gctccgacgc
     2881 gaacgggccg atgatgtgaa cgccgagaat ctcgtcggtt ttcgacgccg caaggacctt
     2941 gacgaggccg ctcgtatcgc cgagggcccg cgcacgcgcg ttcgccgaga acgggaatac
     3001 gcccgcgcgg tactcgacgc gctccgcttt cagctgctgc tcggtcttgc cgacccaggc
     3061 gatctcgggc gcggtgtaga tgacccacgg cacggtgttg aagtcgaagt gcggcttctg
     3121 gccggcgatg cgctcggcta cggccacgcc ctcctcctcg gccttgtgcg ctagcatcgg
     3181 cccgcgcacg acatcgccga tcgcccagac gttgggaagg ttggtgcggc attcgtcatc
     3241 gacttcgatg aaaccgcgct cgtcgagctt caagcccaca ccggcggcat tcagtcctgt
     3301 ggtattgggc acccggccga tgcaaacgat cagccggtca cactccaagg tctggcttga
     3361 atttccggat acagtgtatt gaactttaac ggttttattc gtttcaactt tggtgatctt
     3421 cgcccccagc tcaa
//
LOCUS       DASTPD010000043         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1448391, whole genome shotgun sequence.
ACCESSION   DASTPD010000043 DASTPD010000000
VERSION     DASTPD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448391"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFR57_01770"
     CDS             <1..360
                     /locus_tag="VFR57_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258132.1"
                     /translation="AQTLILDPEIILMDEPFSALDIQTRQLMENELLELWAANRKSVV
                     FITHDLEEAISLSDRVVVLAAGPESHPIGEFAIDLPRPRDVAEIRLTPRFIELHTQIW
                     HAMKAEVLKSYAQQQKH"
     gene            338..1159
                     /locus_tag="VFR57_01775"
     CDS             338..1159
                     /locus_tag="VFR57_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258133.1"
                     /translation="MPSSRSTKAALRFWQLALLTGVLVFWYVATSPTLLPPIYFDDPH
                     KAAFFFGEPLLVLARIWNWFSTGFIYPHLGVTLIETALAFVIGTAFGLVMGLWLALSP
                     TASSLLDPYIKAVNSMPRVILAPIFGVWFGLGIASKVALGVTLVFFIVFFNVYQGVRE
                     VSYTVLANARILGASDRQLLRHVYVPSAMSWVFASLHNSVGLAFVGAVVGEYLGSSRG
                     VGYVILQAEGTFDINSVFAGILVLTAFALLLDWMVTLAERRLLVWQPKGGETEKI"
     gene            1156..2100
                     /locus_tag="VFR57_01780"
     CDS             1156..2100
                     /locus_tag="VFR57_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258134.1"
                     /translation="MKLLLALLVAVPALVHAYPTKPVRVVVPYPAGSTPDIIGRTLAE
                     RLQKSYGQPFVVENRSGAGGNIGAEVVAKAGDPHVLLIGGNGPVAINKHLYKGLAFDP
                     DRDLVPISLLATAPQMLVVRNDIGIGDFKTFVEHARRNPGRLSYASVGGGSASHLTME
                     LLKSDARLFLVHIPYRGFPPAVTDMLGGNIDAMFAIIPAVLPHVRAGKMKPLAVTALT
                     RNNLAPDVPSVAELGLSQLESLAWNGLLAPSGTPPAIVERLVVETVNAMRDREAREAL
                     ARLGFDVVASSPQEFSRWIRAESEKWARVIRASGATAD"
     gene            2203..2279
                     /locus_tag="VFR57_01785"
     tRNA            2203..2279
                     /locus_tag="VFR57_01785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2237..2239,aa:Met,seq:cat)
     gene            2452..>2536
                     /locus_tag="VFR57_01790"
     rRNA            2452..>2536
                     /locus_tag="VFR57_01790"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          430 a          818 c          787 g          501 t
ORIGIN      
        1 gcgcaaacgc tgattctcga tcccgagatc atcctgatgg acgagccgtt ctcggcgctc
       61 gacatccaga cgcgccagct catggagaac gagctcctcg agctctgggc ggccaaccgc
      121 aagtcggtgg tcttcatcac gcacgacctg gaggaggcga tttcgctttc cgaccgggtg
      181 gtggtgctcg ccgccggccc cgagtcgcac ccgatcggcg agttcgcgat cgacctgccg
      241 aggccgcgcg acgtcgccga gatccgtctc acgccgcgct tcatcgagct gcacacgcag
      301 atctggcacg cgatgaaggc cgaagtgctg aagagctatg cccagcagca gaagcactaa
      361 ggccgcgctg cgcttctggc agctcgcgct gctcactggc gtgctcgtct tctggtacgt
      421 ggcgaccagc ccgacgctcc tgccgcccat ctacttcgac gatccgcata aagccgcgtt
      481 cttctttggc gaaccgctgc tcgtgctcgc gcgcatctgg aactggttct ccaccggttt
      541 catctatccg catctcggcg tgacgctgat cgagaccgcg cttgctttcg tcatcggcac
      601 cgcgttcgga ctcgttatgg ggctttggct tgcgctcagc cctaccgcct cctcgcttct
      661 cgatccctac atcaaggcgg tgaactccat gccgcgcgtg atcctcgcgc cgatcttcgg
      721 cgtttggttc ggcctcggca tcgcgtcgaa ggtggcgctt ggcgtgacgc tcgtcttctt
      781 catcgtgttc tttaatgtct accagggcgt gcgcgaggtg agctacacgg tgctcgcgaa
      841 tgcacgcatc cttggcgcga gcgaccggca gctcctgcgc cacgtctacg tgccgagcgc
      901 gatgagctgg gttttcgcga gcctccacaa ctccgttggc ctcgccttcg tcggcgcggt
      961 ggtcggcgag tacctcggct cctcgcgcgg cgtgggctac gtgatcctgc aggccgaagg
     1021 caccttcgac atcaactccg ttttcgccgg gattctcgtc ttgacggcgt tcgcgctcct
     1081 tctcgactgg atggtcacgc tcgcggagcg gcggctgctc gtgtggcagc cgaagggcgg
     1141 cgagacagaa aagatatgaa gcttctgctt gcgctcctcg tggcggttcc ggccctggtg
     1201 catgcgtatc cgaccaaacc cgtgcgcgtg gtggtgccgt atccagcggg ctccacgccc
     1261 gacatcatcg gccgcacgct cgccgagcgc ctgcagaaga gctacggtca accgtttgtc
     1321 gtggagaacc gcagcggcgc cggcgggaat atcggcgccg aggtggtcgc caaggcgggc
     1381 gatccgcacg tgctcctcat cggcggcaac gggccggtgg cgatcaacaa gcacctctac
     1441 aaggggcttg ccttcgatcc tgacagggac ctcgtgccga tctcgctcct cgcgacggcg
     1501 ccgcaaatgc tggtggtgcg caacgacatc ggcatcggcg atttcaagac gttcgtggag
     1561 cacgcgcgcc gcaaccccgg ccgtctctcg tacgcctcgg tgggcggcgg cagtgcgtcg
     1621 catctcacca tggagcttct gaaaagcgac gcacgcctct tcctcgtcca catcccgtat
     1681 cgcggatttc cgccggcggt gaccgacatg ctgggcggaa acatcgacgc gatgttcgcg
     1741 atcatccccg cggtgctccc gcacgtgcgc gccggcaaga tgaagccgct cgccgtcacc
     1801 gcgctcacga ggaacaacct cgcgcccgat gtgccgagcg tcgcggagct cgggctatcg
     1861 cagctcgagt cgctcgcctg gaacggtctc ctcgcgccaa gcggaacgcc gcccgcgatc
     1921 gtcgagcgcc ttgtcgtcga aacggtgaac gccatgcgcg atcgcgaggc gcgcgaggcg
     1981 ctcgcaagac tgggcttcga cgtcgtggcg agctcgccgc aggaattctc gcgctggatt
     2041 cgcgcggagt cggagaaatg ggcgcgtgtc atccgcgcaa gcggcgcgac ggccgattga
     2101 acttcccgaa agcaccacag tcagatcgca acgcgacgat ttttgggtcg taaatcgttg
     2161 acatggttta aaagagatcg ctataatctt ggtctcttcg ggcgcggggt ggagcagtct
     2221 ggcagctcgt cgggctcata acccgaaggt cagaggttca aatcctctcc ccgcaaccag
     2281 ctctttaaaa attcgcagcc gataggtgtg ggcgcttgaa cgcgaggcgc tgcgcgacgt
     2341 tgggttttgc tttttccctt cgtcatagcg gccaaagcat caagcagtcc acgaaaagta
     2401 atgagagaag taaacctcgt ttactttgag tgagtgcagc aaatcaggat ttaattgaag
     2461 agtttgatcc tggctcagat tgaacgctgg cggcatgcct tacacatgca agtcgagcgg
     2521 cagcgcgggg gcaacc
//
LOCUS       DASTPD010000044         7886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_19790, whole genome shotgun sequence.
ACCESSION   DASTPD010000044 DASTPD010000000
VERSION     DASTPD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19790"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFR57_01795"
     CDS             <1..266
                     /locus_tag="VFR57_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEU0258135.1"
                     /translation="LFVKLRSGYTLEDALEERIRHEIRRHASPRHVPARIVQVADIPR
                     TKSGKIVELAVKAAVHGMPVAHTEALANPEALEFFRDLAELQS"
     gene            complement(263..697)
                     /locus_tag="VFR57_01800"
     CDS             complement(263..697)
                     /locus_tag="VFR57_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0258136.1"
                     /translation="MPDRSIRVIIEDQKPVTAKSNVTVAAAARLMKQHRIGAVLVVDH
                     GRLSGIFTERDALFRVIAEGRDPEKTRVAEVMTLNPRTIEPDRPFGHALHLMYEGEFR
                     HVPVVENGRPLGMVSARDALGPDLQQFIADLETRVHIGEILG"
     gene            complement(776..1147)
                     /locus_tag="VFR57_01805"
     CDS             complement(776..1147)
                     /locus_tag="VFR57_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEU0258137.1"
                     /translation="MKEGIVLFAHGSRDPEWARPFERISQEVARRRPQAVVCSAYLEL
                     MRPTFDEAVAALAAAGVSSIRVVPLFLGPGGHVKEDLPQLVERAGAARPGLSIRLEKP
                     IGEQAQVIEAIAAAIAGGMSR"
     gene            complement(1167..1832)
                     /locus_tag="VFR57_01810"
     CDS             complement(1167..1832)
                     /locus_tag="VFR57_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0258138.1"
                     /translation="MSSGRVFRFAPTPERPPNHLFVFLHGCGATARSMLPIAFKFQAR
                     FPSAVLVLPSGFDPEARGGAAQDWYPTRGLNCYNHRERVAAVLPDVEDLVRREQTNFG
                     VPSSRTVLIGFSQGGTVALEAVKSPGIAGAVVAFSSRFAQTPGPGSRISSRIHLVHGE
                     FDSVVSRVYAERAARVLAGLHVPVTLDIVEDLGHALSHRAISLGSLRLLQGIYEGRRQ
                     TLH"
     gene            1896..3590
                     /locus_tag="VFR57_01815"
     CDS             1896..3590
                     /locus_tag="VFR57_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0258139.1"
                     /translation="MQTTGFLRAFWALTKPYWVSRQRAKGLTLLATVVGLSLVTVWLE
                     VKFNYWNRDFYNTFENRDQAEFFRQLGMFALLALMWIVTRVYRLYFLQMLQIEWRAWL
                     TDRFLGEWMQDQAHYRMQLLDRGTDNPDQRIAEDLRLFVELTSDLILGLLEAVVLLIS
                     FIIILWSLSGSLELFGISIPGYLVWVALVYAAVGTLLTHFIGRKLIGLDFNQQRVEAD
                     FRFSLVRLRENSEGVALYRGETEELSGLRGRFAAVIANFWQIMYKRKQLGWFTSFYYQ
                     LAIIFPYVVVAPRFFSGTIPLGAIFQTASAFNQVQGSLSWFINAYVMFASWKATVDRL
                     LGFSASLERVRSEAEKLDGERVEGSDNSLVVEDLAIALPQGAPLLGRTALQLKPGENV
                     LLTGPSGAGKSTLFRALSGIWPYWKGRIRLPRGARLLFLPQKAYLPIGTLKRTVCYPD
                     DPAQHSDESVRETLAHVGLAHFAGDLERSENWSQVLSGGEQQRLAFARALLTRPDWLF
                     LDEATASLPEADQDRLYRLLRDELKRTTIVSIGHRESLAKYHAKRLDWRESALVPA"
     gene            complement(3587..4417)
                     /locus_tag="VFR57_01820"
     CDS             complement(3587..4417)
                     /locus_tag="VFR57_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0258140.1"
                     /translation="MLDSLVETRDGQRIATRFFRPQRKPKGSVLIVPAMGVTQDYYQP
                     FASWLATQGFIAATFDYRGTGLSRPRALRGFKADIFDWARLDCAAMIDALPPPLYWVG
                     HSLGGQILPLVPNKDAIKKAVTVATGSGYWRENSPQIRTLAWWLWFVVTPPVLALWGY
                     FPGKALRMVGDLPKGVMAQWRRWCLDPEYAVGAEGEAVRAQYAAVRSPIVSISFSDDE
                     MMSARNTESIHSFYSSAPRTLKRIAPRDIGVVRIGHFGFFRAAFEFSLWRPHLLPELS
                     "
     gene            complement(4402..4845)
                     /locus_tag="VFR57_01825"
     CDS             complement(4402..4845)
                     /locus_tag="VFR57_01825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0258141.1"
                     /translation="MALAEAGRTMPGIEFLRAIRDGRLPAPPIAKLLAFNLVEVEPGH
                     AVFELVPGEQHYNPIGVVHGGVAMTLLDSAMGCAVQTQMPAGGGYTTLEAKTNLVRAI
                     TSATGRLRAIGKLVNLGKRVATAEGRLEDGQGKLYAHATTTCLIL"
     gene            complement(4910..5791)
                     /locus_tag="VFR57_01830"
     CDS             complement(4910..5791)
                     /locus_tag="VFR57_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0258142.1"
                     /translation="MALTLPLPRISTNVRFPEFAVAIAERLFLTPPKPRDAAAAAVDL
                     IDARSSVLEHKGRHIAMWRWGPRDAPAVLLAHGWGGHAAQMRAFVFPLLEAGYRVIAY
                     DQAAHGVSEGRLTGLPDFADVLAEVAWHHGEVKAVIGHSLGGAGAAVALGWGKVQLEK
                     IVLVSPPADLVGYSRRFARWYWIPERVRKGMQRAIEERYGVRWKQLEVPCIAPRLTAQ
                     ALVIHDRDDRMMPWLHGATVARHWPGARLMSTTGLGHRRILADEAVTRAAADFIAGRS
                     AVASPARPAVPYPASLY"
     gene            complement(5808..6440)
                     /locus_tag="VFR57_01835"
     CDS             complement(5808..6440)
                     /locus_tag="VFR57_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0258143.1"
                     /translation="MMALSKGEQTRVAILDEALKIASKLGLEGLTIGSLADATGMSKS
                     GLFAHFGSREDLQLAVLEHGAQQYGERVFVPVLKIERGLPRLRALFERWLHWALESGL
                     PGGCIMISAAIEYDDRPGPIRDAAIANQHRGNAITQKAVRLAVEEGHLSADVDPEQIS
                     FEMLGIVLAAHNHRRLLGDKEARKRALTAFEQLISRHATLAQPRARTATR"
     gene            complement(6437..>7886)
                     /gene="uvrA"
                     /locus_tag="VFR57_01840"
     CDS             complement(6437..>7886)
                     /gene="uvrA"
                     /locus_tag="VFR57_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0258144.1"
                     /translation="EFLVNVGLEYLSLERSAETLSGGEAQRIRLASQIGSGLTGVMYV
                     LDEPSIGLHQRDNARLIATLKQLRDLGNSVIVVEHDEEAIRAADHVIDMGPGAGEAGG
                     RIVAQGSPAEVIANPQSLTGRYLAHELAITVPPQRRRTKRKLLLYGACANNLKEVDLE
                     VPLGLLVCVTGVSGSGKSTLVNDTLYAALARELHGAAAEPAPHRGLAGIEHIDKAIAV
                     DQSPIGRTPRSNPATYTGLFTPIRELFAGVPEARTRGYDAGRFSFNVKGGRCEACQGD
                     GVTRVEMHFLPDIYVACDVCHGKRYNRETLEIRYKGRNIQEVLEMTVDEARGFFSAVP
                     PIARKLQMLADVGLGYVRLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEPTTG
                     LHFHDIKLLLQVLHRLRDAGNTVLVIEHNMDVIKTADWVIDLGPEGGDAGGRIVVAGT
                     PEEVSRVEASHTGRFLHRVLTFTNVRSIFRTA"
BASE COUNT         1304 a         2601 c         2612 g         1369 t
ORIGIN      
        1 tgctcttcgt gaagctgcgc tccgggtaca cgctcgagga cgcgctggaa gagcgcatcc
       61 gccacgagat ccggcgccat gcgagcccgc gccacgtgcc tgcgcgcatc gtgcaggtcg
      121 ccgatatccc gcgcaccaag agcggcaaga tcgtggagct cgcggtcaag gccgcggtgc
      181 acggtatgcc ggtcgcgcac accgaagcgc ttgcgaaccc ggaggccctc gagttcttca
      241 gggatctcgc cgagctacaa agctagccca ggatctcgcc gatgtgcacg cgcgtttcga
      301 ggtcggcgat gaactgctga aggtcggggc cgagcgcatc gcgcgccgac accatgccga
      361 gcgggcggcc gttttcgact accggcacat ggcggaactc gccttcgtac atcaggtgaa
      421 gggcgtgccc gaagggccgg tcgggctcga tggtgcgggg gttcagggtc ataacctcgg
      481 caacgcgtgt tttctctgga tcgcgtcctt cggctatgac gcggaacagc gcgtcgcgct
      541 cagtgaaaat gcccgagagc cggccgtgat ctaccaccag caccgctccg atccgatgct
      601 gcttcatcag gcgggcggct gcagccacgg tgacgttcga cttcgcggtc acaggcttct
      661 ggtcctcgat gatcacgcga atggatcggt ctggcatgat ggccctccac ggcgcacaaa
      721 tttactccca acacgcgcta ttgcaccgca catcttgttt tgcgttgcag taatctcagc
      781 gagacattcc tccggcgata gccgcggcaa tcgcctcgat cacctgcgct tgctcgccga
      841 tcggcttctc cagccggatg ctgagcccgg ggcgggcggc ccccgcgcgc tcgaccagct
      901 gcggcagatc ctccttgacg tggccgcctg ggccaaggaa aagcggcacg acgcggatgg
      961 aacttacgcc ggccgccgcg agtgccgcca ccgcttcgtc gaaggtgggt cgcatcagct
     1021 cgaggtacgc tgagcaaacc acggcctgag gacgcctgcg cgccacctcc tgggagatgc
     1081 gctcgaacgg ccgcgcccac tccgggtcgc gcgagccgtg cgcgaagagg acaatccctt
     1141 ccttcaaatg caggcgctcc agtgctctag tggagagtct ggcgccggcc ctcgtagata
     1201 ccttgcagca accgcagcga ccccaggctg atggcgcggt gggagagcgc gtggccaagg
     1261 tcctcgacga tgtcgagggt taccggcacg tgcagcccgg ccagcacgcg ggcggcgcgc
     1321 tcggcgtaga cgcgcgagac taccgaatcg aactcgccgt gcacgaggtg gatacgcgac
     1381 gaaatgcggc tgccggggcc gggcgtctgc gcgaagcgcg acgagaatgc caccactgcg
     1441 ccggcgattc ccggcgactt gaccgcttcc agcgccaccg tgccgccctg cgagaagccg
     1501 atcagcacgg tgcggctgga gggtacgccg aagttggtct gctcgcgccg cacgagatcc
     1561 tcgacatcgg gcagcaccgc ggcgacgcgc tcgcggtggt tgtaacagtt caagccgcgc
     1621 gtcggatacc aatcctgcgc tgcgccgccg cgcgcctccg gatcgaagcc gctcggcagc
     1681 actagcaccg cgctcgggaa gcgcgcctgg aacttgaacg cgatcgggag catggaccga
     1741 gcggtcgcgc cgcagccgtg caggaagacg aagagatggt taggcgggcg ctctggcgta
     1801 ggggcgaagc gaaaaacccg gcccgaagac attgcgcgat tcttaacccg cggtcaccgt
     1861 tctgtaaact gatttgcatc ccctctgagg tgaccatgca aaccacagga tttctaaggg
     1921 ctttctgggc cctcacgaag ccgtattggg tgtccaggca acgcgccaag gggctgacgc
     1981 tattggccac cgtggtgggg ctgtcgctcg tcaccgtctg gctcgaagtg aagttcaatt
     2041 attggaaccg cgatttctac aacaccttcg agaaccgcga ccaggcggaa tttttccgcc
     2101 agctcggcat gttcgcgctg ctcgctctca tgtggatcgt cacccgggtg taccggctgt
     2161 acttcctgca gatgttgcag atcgagtggc gtgcttggct taccgaccgg ttcctcggcg
     2221 agtggatgca ggaccaggcg cactaccgca tgcagctgct cgaccggggc accgacaacc
     2281 cggatcagcg catcgccgag gacttgaggc tcttcgtcga gctcactagc gaccttatcc
     2341 tggggctgct ggaagccgtg gtgttgctga tttccttcat catcatcctg tggagcttgt
     2401 ccgggtcgct cgagctcttc gggatctcga tccccggcta tctcgtctgg gtggcgctgg
     2461 tctatgccgc cgttggcacg ctgctcacgc atttcatcgg ccgcaagctg atcggcctgg
     2521 atttcaacca gcagcgcgtg gaggcggatt tccgcttctc gctggttcgc ctgcgcgaga
     2581 acagcgaggg cgtcgcgctc taccgcggcg agacggagga gttgagcggc ctgcgcggtc
     2641 gcttcgctgc ggtcatcgcc aacttctggc agatcatgta caagcgcaag cagcttggct
     2701 ggttcacctc gttctactac cagctcgcga tcatctttcc gtacgtcgtg gtggcgccgc
     2761 gcttcttctc cggcacgatt ccgctcgggg cgatcttcca gaccgcttcg gcgttcaatc
     2821 aggtgcaagg gtcgctctcc tggttcatca atgcgtacgt catgttcgcc tcctggaagg
     2881 cgaccgtgga ccgcctgctc ggcttcagcg cgtcgctcga gcgggtgcgc agcgaggccg
     2941 aaaagctgga cggcgaacgc gtcgagggat ctgacaactc tctggtcgtg gaagacctgg
     3001 cgatcgcgct gccgcaaggc gcaccgcttc tcgggcgcac agcgctccag ttgaagcccg
     3061 gcgagaacgt gctgctgacc gggccttcgg gcgcgggcaa gtcgaccttg ttccgcgcgc
     3121 tgtcgggcat ctggccttac tggaaaggtc ggatccgcct gccgcgcggg gcacggctgc
     3181 tgttcctgcc gcaaaaagct tatctgccga tcggaacgct gaagcggacg gtatgctatc
     3241 ccgacgaccc cgcgcagcac tcggacgagt cggtgcgcga gacgctggcg cacgtcgggc
     3301 tcgcgcattt cgcgggcgac ctggaacgaa gcgaaaactg gtcgcaggta ctttcgggcg
     3361 gcgagcagca gcgcctggcg ttcgcgcgcg cgctgctcac gcgtccggac tggctcttcc
     3421 tcgacgaagc gaccgcttcg ctacccgagg ccgaccagga tcggctctac cgactgctgc
     3481 gggacgagct caaacgcacc actatcgtgt ccattggaca ccgcgagagc ctcgccaagt
     3541 accacgcgaa gcggctggac tggcgcgagt cggcgctcgt cccagcctag ctcagctcgg
     3601 gcagcaggtg cggccgccag agcgagaact cgaacgcggc gcggaaaaag ccgaagtgcc
     3661 ctattcgtac gacgccgatg tcgcgcggcg cgatacgctt cagggtccgc ggcgcggagc
     3721 tgtagaagct gtgtatcgat tcggtgtttc gcgcggacat catctcgtcg tccgagaatg
     3781 aaatcgagac gatcggtgaa cggaccgcgg catattgcgc tcgcaccgcc tcaccctcgg
     3841 cgccgacggc gtactccgga tcgaggcacc agcgccgcca ctgcgccatc acgcctttcg
     3901 gcagatcgcc caccatgcgc agcgccttgc ccgggaaata gccccagagc gcgagcacgg
     3961 gcggcgtcac gacgaaccac agccaccacg ccagcgtccg gatctgggga gagttctcgc
     4021 gccagtagcc gctgccggtg gcgacagtca ccgccttctt gattgcgtct ttgttcggca
     4081 cgagcggcag gatctggccg ccgaggctat ggccgaccca gtacagcggc ggcggcagcg
     4141 catcgatcat cgcggcgcag tccaaacgcg cccagtcgaa gatgtcggcc ttgaagccgc
     4201 gcagcgcgcg cgggcgcgaa agtccggtgc cgcgatagtc gaaagtcgcg gcgatgaagc
     4261 cttgggttgc aagccagctg gcgaacggct ggtagtagtc ctgagtcact cccatcgccg
     4321 gcacgatgag gaccgagcct ttcggcttgc gttgcggccg gaagaagcgc gtcgcgatgc
     4381 gctggccgtc ccgagtctcg actagagaat caagcatgtc gtcgtcgcgt gcgcgtagag
     4441 cttgccctgg ccgtcctcca gtcttccctc ggcggtggcc acgcgcttgc caaggttcac
     4501 gagcttcccg atcgcgcgca gccggccggt tgccgaggtg atcgccctga cgaggttggt
     4561 cttggcctcg agcgtggtgt agccgccgcc cgccggcatc tgcgtctgca ccgcgcagcc
     4621 catcgccgag tcgaggagcg tcatggcgac accgccgtga acgacaccga tcgggttgta
     4681 gtgctgctcg cccggcacga gctcgaacac cgcatggccc ggctcgacct cgaccagatt
     4741 gaatgcgagg agcttcgcga tcggcggggc aggcaagcgg ccgtcgcgga tcgcgcgcag
     4801 aaactcgatt cccggcatcg tccgcccggc ctcggcgagc gccatggggt cgctccagga
     4861 tatcgaaaca ctgcgcagat tctttcctgc gtccatcgtg ttctccgggc tagtaaaggg
     4921 aagcggggta gggcaccgcg ggccgcgccg gactcgcaac cgccgagcgc ccggcgataa
     4981 agtcggccgc cgcccgggta acggcttcgt ccgcaaggat gcgccggtgc ccaaggccgg
     5041 tggtgctcat gagccgcgcg cccggccagt ggcgcgcaac ggtcgcaccg tgcagccagg
     5101 gcatcatgcg gtcgtcgcgg tcgtggatca cgagtgcctg cgcggtaagc cgcggcgcta
     5161 tgcagggaac ttccagttgt ttccagcgca ctccgtagcg ctcttcgatc gcccgttgca
     5221 tccccttgcg cacgcgttcg gggatccaat accagcgcgc gaagcggcgc gaatagccga
     5281 cgagatcggc cggggggctc acaagcacga ttttttcgag ttggactttc ccccagccca
     5341 atgcgaccgc ggcgcctgcg ccgcccaggg aatgtccgat caccgccttc acctcgccgt
     5401 gatgccaggc cacctcggcc agcacgtcgg cgaagtcggg cagccccgta agccgtccct
     5461 cggacacgcc gtgcgctgct tggtcgtagg cgatcacccg ataacccgcc tcaagcagcg
     5521 gaaacacgaa agcccgcatc tgtgccgcat ggccgcccca gccgtgtgcg agtagcaccg
     5581 ccggtgcatc gcgcggcccc caccgccaca tggcgatgtg tcgtccctta tgctcgagca
     5641 cactggatcg cgcatcgata aggtcgaccg cggcggccgc ggcatctctc ggcttcggcg
     5701 gcgtgaggaa cagcctctcg gcgatcgcga cggcgaactc cgggaatcga acgttcgtgc
     5761 ttattctggg gagaggaaga gtcaaggcca tggggcttcc tttcggttta gcgggtggcg
     5821 gtgcgggcgc ggggctgcgc caacgtcgcg tggcgggaaa tgagctgctc gaaggctgtg
     5881 agcgctcgct tacgtgcttc cttgtccccc aagagccgcc ggtgattgtg tgccgcgagc
     5941 acgataccca gcatctcgaa gctgatctgt tcggggtcga catcggcgct caggtggccc
     6001 tcttcgaccg ccaggcgcac cgctttctgc gtgatcgcgt tgccacggtg ctggttggca
     6061 atggcggcat cgcggatcgg cccggggcgg tcgtcgtact cgatggcggc ggagatcatg
     6121 atgcagccgc cgggcaaccc tgattccagg gcccagtgga gccagcgctc gaacagcgcc
     6181 cgcagccgcg gcagcccgcg ctcgatcttg agcaccggca cgaacaccct ctcgccgtat
     6241 tgctgggcgc cgtgctccag cacggcgagc tgcagatcct cgcgcgatcc gaaatgggcg
     6301 aagagcccgg actttgacat gccggtggcg tcggccaggc tgccgatcgt gagtccttcg
     6361 agaccgagct tgctcgcgat cttcagcgcc tcgtccagga tcgcgactcg ggtctgttcc
     6421 cctttgctga gagccatcat gccgtgcgaa aaatagaacg aacgttcgta aatgtcaaga
     6481 cccggtgaag gaaccggccg gtatggctgg cctcgacccg cgaaacctcc tccggcgtgc
     6541 ccgccacgac gatccgtccg ccggcatccc cgccctctgg gccgaggtcg atgacccagt
     6601 cggcggtctt gatcacgtcc atgttgtgct cgatgacgag caccgtgttg ccagcgtcgc
     6661 gcagccggtg cagcacctga agcagcagct tgatgtcgtg gaagtgcagg ccggtggtgg
     6721 gctcgtccag tatgtagagg gtgcggccgg tgtcgcgctt cgatagctcg agcgagagct
     6781 tcacccgctg tgcttccccc ccggaaagtg tggtcgcgga ctgtccgagc cgcacgtatc
     6841 cgaggcctac gtcggcgagc atctggagct tccgggcaat cggcggcacc gccgagaaga
     6901 atccgcgcgc ctcgtcgacc gtcatctcca gcacctcttg tatgttccgt cccttgtaac
     6961 ggatctcgag cgtctcgcgg ttgtagcgtt tgccgtggca gacatcgcag gcgacgtaga
     7021 tgtccggcag gaagtgcatc tccacgcggg tcacgccgtc gccctggcag gcttcgcagc
     7081 gcccgccctt cacgttgaac gagaaccgcc cggcgtcgta gccacgggtg cgcgcctcgg
     7141 gcacgccggc gaaaagctcg cggatggggg tgaagagtcc ggtgtaggtg gccggattgg
     7201 agcgcggcgt gcggccgatt gggctctggt ccacggcgat cgccttgtcg atgtgctcga
     7261 tgccggcaag gccgcggtgc ggcgccggct ccgcggcggc accatgcagc tcgcgggcga
     7321 gcgccgcgta caaggtgtcg ttgacgagcg ttgacttgcc ggatccggac acgccggtga
     7381 cgcagacgag cagcccgaga ggcacctcga gatccacctc cttcagattg tttgcgcacg
     7441 cgccgtacag caggagcttt cgcttggtac ggcggcgctg gggcggcacc gtaatcgcga
     7501 gctcgtgcgc gaggtaccgg ccggtgagtg actgcggatt agcgatgact tcggcgggag
     7561 atccctgcgc cacgatccga ccacccgctt cgccggcacc cggacccatg tcgatcacat
     7621 gatcggcagc gcggatcgcc tcttcgtcgt gctcgacgac gatcacggag ttgccgagat
     7681 ctcgcagctg cttgagcgtc gcgatgaggc gcgcgttgtc gcgctgatgc aggccgatcg
     7741 acggctcgtc gaggacgtac atcacccccg tcaggcccga gccgatctgg ctcgcgagcc
     7801 ggatgcgctg cgcctcgccg ccggaaagcg tctcggccga gcgctcgagc gagaggtatt
     7861 cgaggccgac gttgaccagg aactcc
//
LOCUS       DASTPD010000045         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1244566, whole genome shotgun sequence.
ACCESSION   DASTPD010000045 DASTPD010000000
VERSION     DASTPD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244566"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFR57_01845"
     CDS             <1..288
                     /locus_tag="VFR57_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258145.1"
                     /translation="PLDPSWATPRDAGIPDLDASIWVGLFAPKGTPQPIVDRINAEVR
                     ETLKLPDVQKRFIESAGGEAVGMSSKEFLARIKADAERYKRVVQKAGVKPG"
     gene            389..1204
                     /locus_tag="VFR57_01850"
     CDS             389..1204
                     /locus_tag="VFR57_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU0258146.1"
                     /translation="MPTGLNFLIGGYTYSEGGLATDPATPLKNANLQIHAPFVAYARS
                     FDAWRKSGKLDLVLATACLSGEAEANGVPVSRDVCGLLDPALRVTVNVFGAPALTLEE
                     FRHYRQDLIVGVGLQVMAPFGQYDPDRLVNLGTNRWAFKPEIGLSKKLGALSVEAALG
                     ATFFTTNDDYFGGRTREQAPIVSTQLHLIYEFRGGRWLAINGTYYGGGRTTLDGEKQN
                     DELSSTRLGATLALPVDRHHSVKLHASTGVSVRYGGDFTTVGAAWQYRWGGGF"
     gene            complement(1215..2273)
                     /locus_tag="VFR57_01855"
     CDS             complement(1215..2273)
                     /locus_tag="VFR57_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0258147.1"
                     /translation="MALQGGGAHGAFTWGVLDRMLQEPNIDIIGVTGTSAGAMNAVVL
                     ADGLLRGGAEGARERLRMFWESIGAMPGFGSLLWPLSGSDAAAVHLEQTPPYLAWDLV
                     ARNLSPYQLNPMNQNPLRAPLDKLVDFERLRAQKDVEVIVCATNVRTARRRTFSNADI
                     STDAVLASACLPDLFPAVQIDGEAYWDGGYTGNPALMPLLVKLPGCDVVMVRIDPVVR
                     DEIPRSVRDIHDRVLEISFNAASWMEMAAVGMLLTFVEHGLLDRERFDRFRFHAILSS
                     DLEKIPASSKLNNYPAFLEHLFNAGRRIGEAWFAEHGGALGKQSSVNLQRLLQANVLA
                     DFLQRQKNAATKEARAAA"
     gene            2419..3504
                     /gene="cax"
                     /locus_tag="VFR57_01860"
     CDS             2419..3504
                     /gene="cax"
                     /locus_tag="VFR57_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEU0258148.1"
                     /translation="MRRILTELRQQPIYWLLVLAPAVVIAEHAAPKAHTMLFVLSVLA
                     IVPLAALLSSATESVAAKTGDTVGGLLNASLGNLTELVIALTALRMGMFELVKASIAG
                     AIVANLLFMLGISFLLGGLRHHVQEYNAASARLQIAMVLLATVTLFVPSALGGAAEFE
                     GTVFLRDLSVGLSILLIAIYALGLLFSLGTHREVFGAKAHEAHGEEPWPLPVALIVLA
                     VATLFVALVSEVFVASVEHAALQFGMSAAFVGFIVVAIVGGAAESFTAIAAARKNRLD
                     LSVGIALGSAAQIALFVAPVLTLASFVIGPSPMDLSFTRGQVAAVFIAMLTAALVANS
                     GKSAWFTGTQMIAVYMVFAITLYLIPS"
     gene            complement(3651..4220)
                     /locus_tag="VFR57_01865"
     CDS             complement(3651..4220)
                     /locus_tag="VFR57_01865"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEU0258149.1"
                     /translation="MTVRALVALVCASLLPVAVFAEPPPHAKAHGWRKMNDPSYVGYT
                     GKKWDHDYGVIKGRCNTAAVGAVLGGAIGGAVGSRADKDQRPTAVVLGTVIGAVIGAK
                     IGHEIDESDRACMGHSLELAGVKTTVVWKNETTGVTYRLTPTRNLASGREACREFTTE
                     LAVGKGKKKNEKVTGVACRHGNGDWVFNA"
BASE COUNT          815 a         1628 c         1592 g          934 t
ORIGIN      
        1 ccgctggatc cgtcctgggc cacgccgcgc gacgccggca tcccggatct cgacgcctcc
       61 atctgggtgg gcctcttcgc gccgaagggc acgccacagc cgatcgtcga tcgcatcaac
      121 gccgaagttc gcgaaaccct caagcttccc gatgtgcaga agcgcttcat cgagagcgcg
      181 ggcggcgagg cggtcggcat gagctcgaag gaattcctcg cgcgcatcaa ggccgacgcc
      241 gagcgctaca agcgcgtcgt gcagaaggcg ggcgttaagc ccggatagcg cgatcgcgcg
      301 ttccgccgca tttgccgccg cgtgcgcggc gtcgctcgca gcgggcccgg ccgccgcgca
      361 gcagctcgag ccgcgcgcgt acgccaacgt gccgaccggg ctcaacttcc tcatcggcgg
      421 ctacacgtat tcggaaggcg ggctcgccac cgacccggca acgccgctga aaaacgccaa
      481 cctccagatc catgcgccgt tcgtggcgta cgcgcgctcg ttcgacgcct ggaggaagtc
      541 cggcaagctc gacttggtgc tcgccaccgc ctgcctgtcg ggtgaggccg aggcgaacgg
      601 cgtgccggtg tcgcgcgacg tctgcggcct gctcgatccg gcgctacgcg tcaccgtcaa
      661 tgtttttggc gcgccggcgc ttacgctgga agagttccgt cactaccgcc aggacctgat
      721 cgtcggcgtg ggcctgcagg tcatggcgcc gttcgggcag tacgacccgg accggctcgt
      781 caacctcggc acgaaccgct gggctttcaa gccggagatc gggctttcga agaagctggg
      841 cgcgctgagc gtcgaagctg cgctcggcgc caccttcttc acgacgaacg acgattactt
      901 cggcggcagg acgcgcgagc aggctccgat cgtctcgacg cagctgcacc tcatctacga
      961 atttcgcggc gggcgctggc tcgcaatcaa cggcacctac tacggcggtg ggcgcaccac
     1021 cctcgacggc gagaagcaga acgacgagct atcgagcacc cggctcgggg cgacgctcgc
     1081 gctgccggtc gaccgccatc actcggtgaa gctgcacgcg agcacgggcg tgtccgtccg
     1141 gtatgggggc gatttcacca ccgtcggcgc ggcgtggcag taccgctggg gcggcggatt
     1201 ctagaccggc tcgctcaggc tgcggcgcgc gcttccttgg tcgccgcgtt tttctggcgc
     1261 tgcaggaagt cggcgagcac gttcgcctgc aggagccttt gcagattgac cgacgattgc
     1321 ttgccgagcg cgccgccgtg ctccgcgaac cacgcttcgc cgatgcgccg gccggcgttg
     1381 aagagatgct cgaggaacgc cgggtagttg ttgagcttgc tggacgccgg gatcttctcg
     1441 agatcgctcg agaggatcgc gtggaagcgg aatcggtcga agcgctcccg gtcgagcagc
     1501 ccgtgctcga cgaaggtcag caacatcccg accgctgcca tctccatcca cgaggcggca
     1561 ttaaacgaga tttccagcac tcggtcgtgg atgtcgcgca cgctgcgcgg gatctcatcg
     1621 cggaccaccg ggtcgatcct caccatgacg acgtcgcagc cgggcaattt cacgagcagc
     1681 ggcatcaatg ccggatttcc cgtatagccg ccgtcccagt acgcctcgcc gtcgatttgc
     1741 acggcgggga agaggtccgg caggcaggcc gacgcgagaa cggcgtcggt tgagatgtcg
     1801 gcgttggaga acgtgcgccg gcgcgccgtg cgtacgttgg tagcgcacac gatgacctca
     1861 acgtccttct gtgcgcgcag gcgctcaaag tcgaccaact tgtcgagcgg ggcgcgcaga
     1921 gggttctggt tcataggatt gagctggtag ggcgagaggt tgcgcgcaac caggtcccat
     1981 gccaggtagg gcggggtctg ctcaaggtgc accgctgccg cgtcgctgcc tgacagcggc
     2041 cagagcaggc tgccgaaacc cggcatggcg ccgatcgatt cccagaacat gcgcagcctc
     2101 tcgcgcgcgc cctcggcccc gccgcggagc aacccgtcgg ccagtaccac cgcattcatg
     2161 gcgcccgcgc tcgtgcccgt gacgccgatg atgtcgatgt tcggctcctg cagcatgcgg
     2221 tcgagcacgc cccaagtgaa agcgccatgg gcgccgcctc cttgaagagc caatgcgatc
     2281 ttgcggcgct gagtgcgcat cccggtcccc tgctttcttt agcaattggt gtgctgtaag
     2341 actaggcgac gcgctgcacc ctcgcttgac gcagatcaag cacgcgcatc gtggctgcga
     2401 tctactggcg caaacaagat gcgccgaatc ctgacagagc tgcgccagca gccgatctac
     2461 tggcttctgg tgctcgcgcc ggcagtcgtc atcgcggagc atgcggcgcc aaaggcgcac
     2521 accatgcttt tcgtgctttc ggtgctggcc atcgtgccgc tcgccgcgct gctcagcagc
     2581 gcgaccgaat cggtggcggc caaaacgggc gacaccgtcg gcggcctgct caacgcgagc
     2641 ctaggcaacc tgaccgagct tgtcattgcc ctcaccgcgc tgcgcatggg catgttcgag
     2701 ctcgtcaagg cctcgatcgc cggcgcgatc gtcgcgaacc tgctcttcat gctgggcatc
     2761 tctttcctgt tgggcggcct gcggcatcac gtccaggaat acaacgccgc gagcgcgcgc
     2821 ctgcagatcg cgatggtgct gctcgccacc gtcacgctct tcgtaccgtc cgcgctcggc
     2881 ggggccgccg agttcgaggg aaccgtgttc ctgcgcgatc tgagcgtcgg gctgtccatt
     2941 ctgctcatcg cgatctatgc gctaggcctg ctcttctcgc taggcacgca tcgcgaggtg
     3001 ttcggcgcca aggcgcacga ggcgcacggc gaagagccct ggcctctgcc cgtcgccttg
     3061 atcgtgcttg cggtagcgac gctgttcgtt gcgctggtca gcgaggtctt cgtcgcctcc
     3121 gtcgagcacg ccgctctgca gtttggcatg tcggccgcct tcgtcggctt tatcgtcgtc
     3181 gccatcgttg gtggagcggc agagtcgttc acggcgatcg cggcagcaag gaagaaccgg
     3241 cttgacctca gcgtaggcat cgctctcggc agcgcggcgc agatcgcgct gttcgtcgcg
     3301 cccgtgctga cgctcgccag cttcgtcatc gggccctctc ccatggatct gagcttcacg
     3361 cgcggacagg tcgcggcggt attcatcgcc atgctgaccg cggccctggt tgcgaactcc
     3421 ggcaagtccg catggttcac cggtacacag atgatcgccg tctacatggt gttcgcgatc
     3481 acgctgtacc tgataccttc ctagccttcg aagaactcct tcaccttgtc gaaccagccc
     3541 ttggcgcgcg ggttgtggcg cgcgatttca ccaccgtcgg cgcggcgtgg cagtaccgct
     3601 ggggcggcgg gttctagacc gattcgctca ggcggcgttg gcgagtcgaa tcaggcgttg
     3661 aagacccagt cgccattacc gtggcgacag gctacgccgg tcaccttctc gttcttcttt
     3721 ttgcctttgc caacggcgag ctcggtggtg aactcccggc aagcctcacg gccggaagca
     3781 aggttgcgcg tcggcgtaag acggtaggtc acaccggtcg tttcgttctt ccagacgaca
     3841 gtcgttttca cgccagccaa ttcgagcgag tgccccatgc aggctcgatc gctctcgtct
     3901 atctcgtggc cgatcttggc gccgatgact gcaccgatca ccgtgccgag cacgaccgcc
     3961 gttgggcgct ggtctttgtc ggcgcgcgac ccgacggcgc cgccaattgc gccgccgagc
     4021 acggcgccca ccgccgccgt gttacatctg cccttaatga cgccgtagtc gtggtcccac
     4081 ttcttccccg tgtacccgac atagctggga tcattcatct tgcgccagcc atgcgccttg
     4141 gcatgcggtg gcggctcggc gaacacggcg actggcagga gacttgcgca gacaagcgcg
     4201 acaagcgcac ggaccgtcat gattccccct tttgcttttt tcagctgggt gagcagtgta
     4261 aaggagcacg aaccaggcgc tgtacgccgc gtgcgaaaac cgtcacgttg cgccgacaat
     4321 ttcggctaac tcaatgcgcc tctagtggta attggccaca agctgccgtg acacgcgggc
     4381 gcctgcgttt gccactgatc gagtgtgcgt ttcttgcgtc gtgcgtacca cccgggggat
     4441 aaggcggact agccgggctg tccgtttttg tcgtactccc ggtcaagaag ttcagcgatg
     4501 tctacgtcca ttaaccgcgt ccctgcggtg cagcgcgttc cgctggcaac ctgaattcgt
     4561 ccaccgggca gctcaatctg gcacggttct atgcaagtcc aatgaccagc agagtcgcgg
     4621 tgaaagcagc gaatgaagtc tttcaccggc gaagattaga tcgcaggcat agacgtgttc
     4681 tatagggaca ggaccgtctg ttcgagcaag gactaacccg tcacctgaaa actacatcgg
     4741 acgtcgtaac gtgctgcgga gtggtggccg gtggataaac ctagtggccg gagtcgtggc
     4801 gctgatgctg cccacggaat cggcgatgag accgagcgga gagtcgagcc tagtgctgca
     4861 acgcttcttc gatagcggcg aaatcgagcg gcttgacgaa gtatccgtgg aatcctgcgt
     4921 gcatcgcttt gctgcgagtc tcgctctccg cgcgtccggt tatggcgta
//
LOCUS       DASTPD010000046         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_837330, whole genome shotgun sequence.
ACCESSION   DASTPD010000046 DASTPD010000000
VERSION     DASTPD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837330"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFR57_01870"
     CDS             complement(<1..1150)
                     /locus_tag="VFR57_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0258150.1"
                     /translation="MPSRKKPEELRSHRWYGVNDLRSFGHRSRTAQMGYERSDYAGKP
                     VIAIINTWSDINPCHTHFRQRVEEVKRGVWQAGGFPVEMPAMSLSEPMQKPTTMMYRN
                     FLAMECEELLRSYPADGAVLMAGCDKTTPALVMGATSMNLPAIYVPAGPMLKGHWREE
                     TLGSGSDSWKYWAELRAGNIGERDWQEIEDGIARSPGTCMTMGTAATMMALGEALGLT
                     LPGASSIPAPDSNHSKMATAAGKRIVQMVWEDLKPRDLLKKESFDNAIVTLMAMGGST
                     NALIHLIAMAGRAGIKLALERFNELSAKTPLLANVRPSGDKYLMEDFYYAGGLRALLN
                     ELRDLLNLDCRTVNGKTLGENLQGARIFNADVIRPRSKPLKASGGLVVL"
     gene            1185..2144
                     /locus_tag="VFR57_01875"
     CDS             1185..2144
                     /locus_tag="VFR57_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258151.1"
                     /translation="MNRLVTFLLLIAASATAAAQSFPTKPIRVIIPFVPGGSSDIVGR
                     AIASKFQEFLGQPAVVENRPGANGAIAAEFVANAEPDGYTVLVGSIGVFSINAALYKD
                     LRYHPVRDFAPITLAVTTPNVLITKPTLAAGSLKELVEQMKKNPGKLSYCSSGPGSSD
                     HLTAELLKQATGTDALHIPYKGGAACQTDIMGNQVDFSFQNLGAVTNYIKANRMKALA
                     VTAKARHPQLPQVPSVAEAGVADLVVTSWQAAAAPAKTPKDAVTRLNGAMVKALRSPD
                     IAERMNQIGFDVVASSPEEFGAFMKGEVERWTGVVAKGNIKPE"
     gene            2144..>2727
                     /locus_tag="VFR57_01880"
     CDS             2144..>2727
                     /locus_tag="VFR57_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0258152.1"
                     /translation="MGFVFKQQSLKKAIAAIAAAGGSDSAEAELVADNLVSANLTGHD
                     SHGIGMMPRYVAALLEGGLHPNRKIEVRLDGGALLALDGGAGYGQSIGLQATHLAIER
                     AKKHGVSITALGNSHHLGRIGHWAEMAVAGGLVSIHFVNVQSFARVAPFAGADRRFGT
                     NPVCIGIPLPGEAPFLHDMATSAVAQGKLRGAYN"
BASE COUNT          476 a          893 c          884 g          474 t
ORIGIN      
        1 gcagcacgac gagaccgccg gaagccttta ggggttttga acgcggccga atgacatcgg
       61 cattgaaaat ccttgcacct tggagattct cgccgagcgt cttgccgttg acggtcctgc
      121 agtcgagatt cagcagatcg cgcagttcgt tcagcagcgc acgcaggccg ccggcgtagt
      181 agaagtcttc catcagatac ttgtcgccgg acgggcgcac gttggcgagg agcggagtct
      241 tcgccgagag ctcgttgaag cgctcgagcg ccagcttgat cccggccctg cccgccatgg
      301 cgatgaggtg gatgagggcg ttcgttgagc cgcccatcgc catcagcgtg acgatggcgt
      361 tatcgaaaga ctcctttttc aagagatctc ggggcttcag atcttcccag accatctgca
      421 cgatgcgctt gcctgcggcg gtagccatct ttgagtggtt ggagtcgggc gcgggaatcg
      481 aggaggcgcc gggcagggtg aggccgagcg cctcgccgag cgccatcatg gtcgccgcgg
      541 tgcccatagt catgcaggtg cccggcgagc gcgcgatgcc gtcctcaatc tcctgccagt
      601 cgcgctcgcc gatattgccg gcgcgcagct ctgcccagta tttccagctg tcggaacccg
      661 agcccagcgt ttcctcgcgc cagtggcctt tcagcatcgg gcccgccggc acgtaaatcg
      721 ccggcaggtt catgctggtc gctcccatga cgagggcggg cgtcgtttta tcgcagcccg
      781 ccatcagcac cgcgccgtcg gcggggtagg agcgaaggag ctcctcgcat tccatggcga
      841 ggaagttgcg gtacatcatc gtggtcggct tctgcatcgg ctcggagagc gacatcgccg
      901 gcatctccac cgggaatccg cccgcctgcc acactccgcg cttcacctct tccacacgct
      961 ggcgaaaatg cgtgtggcag ggattgatgt ccgaccaggt gttgatgatc gcgatcaccg
     1021 gcttgccggc gtagtcgctg cgctcgtagc ccatctgcgc ggtgcgcgag cggtggccga
     1081 aggaacgcaa gtcgttgacg ccgtaccagc ggtgcgagcg cagctcttca ggcttcttgc
     1141 gggacggcat gggagagggc gattctatca agtagacttc gccaatgaac cgactagtta
     1201 ctttcctcct gttgatcgcg gcgagcgcaa cggccgccgc ccaatcgttc ccgacgaagc
     1261 ccatccgggt gatcattccc ttcgtgcccg gaggctcctc cgacatcgtc ggccgagcga
     1321 tcgcgtcgaa attccaggag tttctgggcc aacctgccgt cgtggagaac cgcccgggcg
     1381 caaacggcgc gatcgcggcg gagttcgtcg ccaatgcgga gcccgacggc tacacggtcc
     1441 tcgtcggctc gatcggcgtg ttctcgatca acgccgcgct ctataaggat ctgcggtatc
     1501 acccggtgcg cgactttgcg ccgatcactc ttgccgtgac cacgccgaat gtgctgatca
     1561 cgaagcccac gcttgcagcc ggctcgctga aggagctggt cgagcagatg aagaagaatc
     1621 caggcaagct ttcctactgc tcgagcggcc cgggttcttc agaccacctt accgcagagc
     1681 tgctcaagca ggccaccggg accgacgcgc tgcacattcc ctacaagggc ggcgccgcct
     1741 gccagaccga catcatgggc aaccaggtcg acttttcgtt ccagaacctg ggcgccgtga
     1801 ccaactacat caaggccaac cgcatgaaag cgctcgccgt caccgccaag gcgcgccatc
     1861 cgcagctccc ccaggtgccc tcggtggctg aagcgggagt cgccgacctc gtcgtcacct
     1921 cctggcaggc cgcggccgcg ccggcgaaga cgccgaagga cgcggtgacc cggctgaacg
     1981 gcgcgatggt gaaagccctg cgctcgccgg atatcgccga gcgcatgaac cagatcggct
     2041 tcgacgtcgt ggcgagctcg ccggaggagt tcggagcttt catgaaaggc gaggtcgagc
     2101 gctggacggg cgtcgtcgcc aagggaaata tcaagcccga gtaatgggct tcgttttcaa
     2161 gcagcagagc ctgaagaagg caattgccgc gatcgccgcc gcaggcggca gcgattccgc
     2221 ggaagcggag ctcgtcgctg acaatctggt cagcgcgaat ctgaccggtc acgactcgca
     2281 cggcatcggc atgatgccgc gctacgtagc agcgctgctc gagggcgggc tgcatccgaa
     2341 ccggaagatc gaagtgaggc tcgacggcgg cgcgctgctc gccctcgacg gcggcgcggg
     2401 ctacggccag tcgatcggcc tgcaggcgac gcaccttgcc atcgagcggg cgaagaagca
     2461 cggcgtctcg atcaccgcgc tcggcaactc gcaccacctc ggccgcatcg gtcactgggc
     2521 cgagatggcg gtcgccgggg gcctggtgtc gatccatttc gtcaacgtgc agtctttcgc
     2581 gcgcgtcgcg ccgtttgcgg gcgccgaccg gcgcttcggc accaacccgg tgtgcatcgg
     2641 catcccgctg cccggcgagg cgccgttcct ccacgacatg gcgaccagcg cggtcgcgca
     2701 aggcaagctg cgcggcgcgt acaacaa
//
LOCUS       DASTPD010000047         4209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_225771, whole genome shotgun sequence.
ACCESSION   DASTPD010000047 DASTPD010000000
VERSION     DASTPD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225771"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..780)
                     /locus_tag="VFR57_01885"
     CDS             complement(154..780)
                     /locus_tag="VFR57_01885"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEU0258153.1"
                     /translation="MTTLVMLSGGIDSTAMLVKLLTASADELRVHHIRMVNREGRDRA
                     ESRAVEAIVAYCRAHYRPFRYCESGLNFSALEAIPIDYLSIAFVACQVAIDTPGCNRI
                     AVGALAADTDIVNRTGRQKRVFQAMYECYRARKLGEPNVEWLFPVYDTPKAELIAALP
                     QALLDLTWSCRRPLDGFRPCGACKACRAREAANLQGLRVPAAAAENRA"
     gene            813..1589
                     /locus_tag="VFR57_01890"
     CDS             813..1589
                     /locus_tag="VFR57_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0258154.1"
                     /translation="MSVKKLLDLSGKVAVVTGGSRGLGLQIAEALGEMGARLALTARK
                     KDELNSAVVHLGNQGIEAVPFVCDIGRREQIAPFAEDAMKLFRKIDILVNNAGATWGA
                     PAETHPLDAWDKLVSVNMSGLFALTQIVARKSMIPAKWGRIVNVASVAGLMGQDPRFA
                     ATAAYTATKHGVVGLTRQLAAEWGVHGITVNAICPGFFPSKMTRATLETAGKLIQEWT
                     PTRRLGGDEDLKGLAVLLASEASRHITGQAIAVDGGATIV"
     gene            1586..2005
                     /locus_tag="VFR57_01895"
     CDS             1586..2005
                     /locus_tag="VFR57_01895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0258155.1"
                     /translation="MNLEFAREYQKKIPFLQHLKIQTDELGKGAARLSLPIERHLTNS
                     LGTAHGGVIMSLLDVALCTAARTLHPDSLGVITIDLSVSFIGGASGARLWADARVLRD
                     GRSMSFVEAEAKNEDGSLVAKAIATVRVRLKEKGGRS"
     gene            2002..2787
                     /locus_tag="VFR57_01900"
     CDS             2002..2787
                     /locus_tag="VFR57_01900"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEU0258156.1"
                     /translation="MTTVNPYAAPKAALADETLSSNADFVPGGQPRPAANGWTWIAEG
                     WELFKRQPGMWIGMILVLLLIFVAAAFIPIVGGLAMTLFGPVFAAGVMLGCKALDAGE
                     QLELSHLFGGFRSRTGALIGVGALYLAATIAVALVVSLVMGVGMMTMMGGGDQQAVAA
                     MGMTFVLGVLIMLALLLPAMMAVWLAAPLVVFHDHGAIDAMKGSFTGCLKNVLPFLLY
                     GVVLALLSILASLPLGLGWLILGPVFAASIYASYRDIYLKPRP"
     gene            complement(2771..4006)
                     /locus_tag="VFR57_01905"
     CDS             complement(2771..4006)
                     /locus_tag="VFR57_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258157.1"
                     /translation="MGSDPIFLRVALPFIAGYFVSYVYRMVNAVLAPTLVAEFGLSAG
                     SLGLLSSMYFLSFALCQLPVGVAMDRFGPRRVNAALLLVAAAGGAWFAHAESAAAAIA
                     ARALIGVGVSACLMASLTAFVLWYPAERISTMNAIAFSAGAVGAMTATVPLELVLRVW
                     PWRETFMLIVAATVAVSLVLWLWVPERSARPRGEKLQEITEGLLQLLRDPAFLRLAVC
                     LGASQFAAVALQTLWIATWLRDVAGYSPAEVARGLLAVNVAMIVGYLGFGRAADAWQR
                     RGRNTVSLLAAGVALSSLSLALLVLGVKLLLLWLIFVGAGTSVVLAYSILSRRLPKAM
                     AGRANTAINVFGFVGMFSGQWGIGLVLDLWPPSASGYAAAAYPWALGMTWAVQLAGLA
                     WLWSGRALLHGNSRATVAA"
     gene            complement(4027..>4209)
                     /locus_tag="VFR57_01910"
     CDS             complement(4027..>4209)
                     /locus_tag="VFR57_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU0258158.1"
                     /translation="LEKRPVPPPPEERARMLQAEDLGKTILFVATLPPRACVNELIIS
                     PTWNRFYLGGVETPQN"
BASE COUNT          770 a         1383 c         1386 g          670 t
ORIGIN      
        1 agaggatcag gaccgagagc tgcttgaagc tctcgacgag cgaatgcgcg atcgtccggc
       61 agcggcgcag gtgcccgagg ccaaacgtgt cgtggctgta gatgagtatg cggccatcgt
      121 tccgcctgga cctcatggcg cccctccccc ctgtcaagcc cgattttcgg ctgccgccgc
      181 gggaacgcgc aagccctgca ggttggctgc ttccctggcc ctgcaggcct tgcatgcccc
      241 gcacggacga aagccgtcca agggccgccg acaggaccat gtcaggtcca gcagcgcttg
      301 cggcagcgcc gcaatgagct cggccttggg cgtgtcataa accggaaaca gccactcgac
      361 gttcggctcg cccagcttgc gcgcgcggta gcactcgtac atggcttgga aaacacgctt
      421 ctgccggcca gtgcgattca cgatgtcggt gtccgcggca agcgcgccga ccgcgattcg
      481 attgcacccc ggcgtatcga tcgcaacctg gcaggcgacg aacgcgatcg aaagatagtc
      541 gatcgggatc gcttccagcg ccgagaaatt caggcccgat tcgcaatagc ggaacggccg
      601 atagtgcgcg cggcaatacg cgacgatcgc ctcgaccgca cggctctcgg cgcggtcgcg
      661 tccttcgcgg ttcaccatgc ggatgtggtg cacgcgcagc tcgtccgcgc tcgcggtaag
      721 cagcttgacc agcatcgcgg tgctgtcgat cccgcccgaa agcatcacca gggtcgtcac
      781 agccagattc tatctgtact aggattcgcc caatgagcgt gaaaaagctg ctcgatctga
      841 gtggaaaagt ggctgtggtg accggcggct cgcgcggttt gggcctgcaa attgcggagg
      901 cgctcggcga gatgggggcc aggctcgccc ttacggcgcg gaagaaggac gaattgaatt
      961 ctgcagtagt tcacctcggg aaccaaggca ttgaggccgt accgttcgtc tgcgacatcg
     1021 gcaggcgaga gcagatcgcg ccgttcgccg aagatgcgat gaagctcttc aggaagatag
     1081 acatcctcgt caacaacgcg ggtgccacgt ggggcgcgcc ggcggaaacg catccgctgg
     1141 acgcatggga caagctcgtc agcgtgaaca tgagcgggct tttcgcgctc acgcagatcg
     1201 tcgccagaaa atcgatgatt ccggcgaagt ggggacgcat cgtcaacgtc gcctcggtcg
     1261 ccgggctgat ggggcaggat ccgcgcttcg ccgcgaccgc cgcctatacc gcgaccaagc
     1321 acggcgtggt cggcctcacg cgccagctcg cggccgagtg gggcgtgcac ggcatcaccg
     1381 taaacgcgat ctgccccggt tttttcccgt cgaagatgac acgcgcaact ctcgagaccg
     1441 caggaaaatt gattcaagag tggactccga cgcgacgcct cggcggcgac gaagatctga
     1501 aggggctcgc cgtactgctc gcctccgaag cctcgcgcca catcacgggg caggcgatcg
     1561 cggtcgacgg cggcgccacc atcgtatgaa cctcgagttc gcacgcgaat accagaagaa
     1621 gatcccgttt ctgcagcacc tgaagatcca gaccgacgag ctgggcaagg gcgctgcgcg
     1681 gctctcgctg ccgatcgagc gccatctcac caacagccta ggcaccgcgc acggcggcgt
     1741 catcatgtcg ctgctcgacg tggcgctctg caccgcggcg cgcacgctgc acccggactc
     1801 gctcggcgtc atcaccatcg acctcagcgt ctcgttcatc ggaggcgcga gcggcgcacg
     1861 gctttgggcg gatgcgcgcg tgctgaggga tgggcgtagc atgagcttcg tcgaggcaga
     1921 ggcgaagaac gaggacggtt cgctcgtggc gaaagcgatc gccacggtac gagtaaggct
     1981 caaggaaaag gggggtcgct catgaccaca gtaaatccct acgccgcacc gaaggcggcg
     2041 ctcgccgacg agaccctaag ctccaatgcc gatttcgtcc caggcggcca gccgcgtccg
     2101 gccgcgaatg gctggacctg gatcgcggag ggatgggagc tcttcaagcg ccagccgggc
     2161 atgtggatag ggatgatcct cgttctgttg ctcatcttcg tcgccgctgc cttcataccg
     2221 atcgtcggcg ggctggcgat gactctcttc ggaccggtgt tcgccgccgg cgtgatgctg
     2281 ggctgcaagg ccctcgacgc gggcgaacag ctcgagcttt cccacctctt cggcgggttc
     2341 cgcagccgca ccggcgcgct gatcggcgtg ggcgcgcttt acctcgcggc caccattgcg
     2401 gtggcgctgg tggtgagtct tgtcatgggc gtaggcatga tgacgatgat gggcggcggg
     2461 gaccagcagg cggtggccgc gatggggatg acgttcgtgc tcggcgtgct gatcatgctg
     2521 gcgctgctgc ttcccgcgat gatggcggtc tggcttgccg cgccgctcgt cgtattccac
     2581 gaccacggcg cgatcgacgc gatgaagggc agcttcacgg ggtgcctgaa gaacgtcctg
     2641 cctttcctgc tctacggcgt cgtgctcgcc ctgctctcca tcctcgcgtc gttgccccta
     2701 ggtctcggat ggctcatcct cggcccggtg ttcgcggcgt cgatttacgc ttcgtaccgc
     2761 gacatctacc tcaagccgcg accgtagcgc ggctgttgcc atggaggagc gcgcggccgc
     2821 tccacagcca tgcgaggccg gcgagctgca cagcccaagt catgccaagc gcccagggat
     2881 aagccgcggc ggcgtagccg ctcgcgctcg gcggccacag atcgagcacc aggccgattc
     2941 cccattgccc gctgaacatg ccgacgaagc cgaaaacgtt gatcgcggtg ttcgcgcggc
     3001 cggccatagc cttcggcagc cgccgcgaca ggatggaata cgcaagcaca accgaggttc
     3061 cggctcctac gaaaatgagc cacagcaaga gcagtttcac gccgaggacg agcaatgcca
     3121 ggctgagcga agaaagcgca acgcctgccg cgagtaacga aacggtattg cgcccgcgcc
     3181 gctgccaggc atccgccgcg cggccaaagc cgaggtagcc gacgatcatc gccacgttga
     3241 cggcgaggag cccgcgcgcg acctctgcgg gcgaatagcc cgcgacgtcg cgcagccagg
     3301 tcgcgatcca cagcgtctgc agcgccaccg cggcgaactg gctcgcgccg aggcacaccg
     3361 cgagccggag aaatgccggg tctctcaaga gttgcagcaa gccctcggtg atctcctgca
     3421 gtttttcgcc tcttggccgt gcgctgcgct ccggcaccca taaccacagc acgaggctca
     3481 cggctacagt ggcggcgacg atgagcatga aggtctcgcg ccagggccag acgcgaagca
     3541 cgagctcgag cggaacggtg gcagtcatcg cgccgaccgc gccggcggag aacgcgatcg
     3601 cgttcatggt cgagatgcgc tcggcggggt accacagcac gaacgcggtg agggaggcca
     3661 tcaggcacgc cgagacaccc acgccgatga gcgcgcgcgc cgcgatcgca gcggcggcgc
     3721 tttcggcgtg cgcaaaccac gcgccgcctg ctgcagcgac gaggagcagc gccgcgttca
     3781 cgcgtcgcgg cccgaagcgg tccatcgcca cccccaccgg aagctggcac agcgcgaacg
     3841 aaaggaagta catgctagag agcaacccca agctgcccgc gctcagcccg aactcggcga
     3901 ccagcgtcgg cgcgagcacc gcattcacca tgcggtacac gtaggagacg aaatagcctg
     3961 cgatgaacgg cagcgctacg cgcaagaaaa tggggtctga ccccattttt ccgtccccat
     4021 ccgtccttag ttctgcgggg tctcgacccc gcccaggtag aagcggttcc aggtcgggct
     4081 gatgatgagc tcgttcacgc aggcgcgcgg cggcagcgtg gcgacgaaca ggatcgtctt
     4141 gccgaggtct tcagcctgca gcatgcgcgc gcgctcttcc ggcggcggcg gcaccggccg
     4201 cttctcgag
//
LOCUS       DASTPD010000048         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1042461, whole genome shotgun sequence.
ACCESSION   DASTPD010000048 DASTPD010000000
VERSION     DASTPD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042461"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..471)
                     /locus_tag="VFR57_01915"
                     /pseudo
     CDS             complement(16..471)
                     /locus_tag="VFR57_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904563.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
     gene            complement(566..913)
                     /locus_tag="VFR57_01920"
     CDS             complement(566..913)
                     /locus_tag="VFR57_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258159.1"
                     /translation="MERYEEEDGRYVLYVGGVRVATTERVALLVDAEHGRLITHGDPV
                     SVRGELDALRAIDPERTAGWLLLEGRPALAALNRALRGNVDIHELHLAFTQASAQRLA
                     GELIARLRQRRMT"
     gene            984..1565
                     /locus_tag="VFR57_01925"
     CDS             984..1565
                     /locus_tag="VFR57_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0258160.1"
                     /translation="MAEKEADAELVRLAQGGDERAFQALVVKYQRRIARHVARYVRRA
                     ADVEEVVQDAFIRAYRGLASFRGESSFYSWLYRIATNAAFSFLKKTAGEVQADERSDE
                     GFEPGLTDEQSPERILLARQIGDAVERAMARLQPELAEALVLFEVEGKSYKEIAQMLG
                     TPIGTVRTRIFRAREFIARRLEPVLGPARDRRW"
     gene            1568..1807
                     /locus_tag="VFR57_01930"
     CDS             1568..1807
                     /locus_tag="VFR57_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HEU0258161.1"
                     /translation="MSLKVSLLLDGQLEPAEVKPALEELAADAALRDRYTLYGLAGDA
                     LRGNPTPDDGFSIRIFERMKREAVKIEPGFDPLKD"
     gene            complement(1804..3621)
                     /gene="typA"
                     /locus_tag="VFR57_01935"
     CDS             complement(1804..3621)
                     /gene="typA"
                     /locus_tag="VFR57_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU0258162.1"
                     /translation="MNRARRNIAIIAHVDHGKTTLVDKLLQQSGTFAAHERVEERVMD
                     SNELERERGITILAKNCAIRYLGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALAQGLKPIVVVNKVDRPGARSDWVVNQTFELFDKLGAAEDQLDF
                     PVIYASALQGWSARNPGETGTNMQPLFDAILEHVPVRDENPDAPLQLQICSLDYSSYV
                     GKIGIGRIRRGRIRPAQQVFLMNGENGASLLTKVNQVLKFEGLERKPVEEAEAGDIVL
                     INGIDEVGIGATLCDPEHPEALPPLKVDEPTLTMNFLVNASPLAGREGRFVTSRQLRE
                     RLERETKSNVALRVEYPADTDTFIVSGRGELHLTILIENMRREGYEVAVSRPRVVCKE
                     IDGEKCEPFELLTVDLEEQNQGAVMEELGRRRGELRDMQPDGKGRVRLDYCIPARGLI
                     GFQGEFMNLTRGTGIMSHVFDGYAPVKGEIPERRNGVLVSQDDGAAVAYALWKLQERG
                     RMFVSPGDPVYEGMIIGIHSRDNDLVVNPIRAKQLTNFRAAGKDDAILLTPPIALTLE
                     YAVEFIGDDELAEITPKSIRLRKRFLKEHERKRAERAAA"
     gene            3721..4743
                     /locus_tag="VFR57_01940"
     CDS             3721..4743
                     /locus_tag="VFR57_01940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU0258163.1"
                     /translation="MDTALLSKTAAAMVAKGKGILAADESSGTCESRFKSVGVECTEE
                     NRRTYRGLLFTTPGVEQYISGVILFDETIRQKANDGVLFPQYLAKKGIIPGIKVDAGA
                     KSLPLHAGEKVTEGLDGLPKRLDEYSKLGARFAKWRAVITIGAGIPSHACIYANAHAL
                     ARYAAACQEASIVPIVEPEVLLDGDHTIERSEEVTEATLRACYAAHSAYNVVPEYVIL
                     KASMVVPGKSCPNQASVEEVAERTVRVLKRTVPASQPGVVFLSGGQSDEAATAHLNAM
                     ASMKGLPWPLTFSYSRALQNPALKTWKGQAGNIAAAQKAFFHRAHMNGLAAQGKWRAE
                     LEKQAA"
     gene            4743..5630
                     /locus_tag="VFR57_01945"
     CDS             4743..5630
                     /locus_tag="VFR57_01945"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0258164.1"
                     /translation="MGALFESNIKSLPRIGKGKVRDIYAVGQDKMLIVASDRLSAFDV
                     VLPDPIPDKGRVLNEMANFWFGRLCHVVANQLTGIDPESVVSEEEKAQVRGRSVVVKK
                     LRPLPIEAVVRGYIIGSGWKDYQKTGKICGIELPKGLRQAEKLPQPIFTPATKAAEGH
                     DENITFAQVEKLIGLELAAKVRDVSIRLYKEAADYAVTKGIIIADTKFEFGLDEHNNL
                     VLIDEVLTADSSRFWPADSYRVGISPPSYDKQYVRDYLESLTWDKTPPAPTLPEDVIA
                     RTSQKYRDALERLTGRKLP"
     gene            5642..6106
                     /gene="purE"
                     /locus_tag="VFR57_01950"
     CDS             5642..6106
                     /gene="purE"
                     /locus_tag="VFR57_01950"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0258165.1"
                     /translation="MGSTSDWETMKAAAKVLEEFAVPYEAKAMSAHRTPHAVAEWATN
                     AQKKGFKAIIAAAGGAAHLAGVIAAHTTLPVLGVPMPSKHLQGLDSLLSTVQMPRGIP
                     VATFAIGEAGAANAALFAVALLALADKQIAGKLEDFRRKQTEAVLKSPPLKL"
     gene            6103..7044
                     /locus_tag="VFR57_01955"
     CDS             6103..7044
                     /locus_tag="VFR57_01955"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0258166.1"
                     /translation="MKPEIRRAAQILRAGGLVAFPTETVYGLGADATNAEAVRKLYAV
                     KRRPADHPVIIHFADAETAFTWAREVPEGARELAGRFWPGPLTLILKRSPLAKDFVTG
                     SQDTVGLRVPSHPVAQELLKEFSGAIAAPSANRFGLVSPTTAAHVRDDLGSEVELVLE
                     GGASEVGIESTILDLSGPSPVLLRPGHIAKDQLREVLALEVFEKALDAPRHPGGMERH
                     YAPRTPAQLVFTYELDKTIAQAKGDVAVLAFSRPDERVDYWLRMPRDPKTYAQRLYAA
                     LRELDTAGCERIIIEAPPLSPEWSAVRDRLKRACGRA"
BASE COUNT         1223 a         2301 c         2356 g         1255 t
ORIGIN      
        1 gactcttgct cctcatcact ttggcataac gactgtatcg atcacatgga tcacgccgtt
       61 cgatgcgacg atatccgtct tgacgactca tgcatcgtcg accctgacga ccccgtcttt
      121 aaccgcaacc gtgagcgact gaccctgcac cgtcttcacg ttgccgggct tcacttgcga
      181 agcggtcacc ttcccgggca cgacgtggta ggtgaggacc ttcgcgagct tttccttgtc
      241 ctggagcagc gcctcgatgg tccccgcggg gagcttggcg aacgcctcgt cggtcggcgc
      301 gaacaccgtg aacgggccct tgcccttcag cgtatcgagg aggcccgccg cctggacggc
      361 cttgacgagg gtgttgaact ggccggccga gacggcggtg tcgacgatgt cggccgcgct
      421 ggcgagcgag gaggcggcgg caagcgccgc ggcagcaaac agctttttca tgggcttcct
      481 ttcggttggg ttgaacgacg ccgcgtccga cctccgccac cggccgccgg tttcagaaaa
      541 aaagctgaaa cctgcccccg gcttgctagg tcattcgccg ttgacgcagc cgcgcgatca
      601 gctcgcctgc caggcgctgc gcgctcgctt gcgtgaacgc gaggtgcaac tcatggatgt
      661 ccacgttccc ccgcagggcg cggttcagcg cagcgagcgc cggccggccc tcgagcagca
      721 gccagcctgc ggtgcgctca gggtcgatcg cgcgcagcgc gtcgagctcg ccgcgcacgc
      781 tgaccggatc gccgtgcgtg atgagccgcc catgctcggc gtcgacgagc agcgcaaccc
      841 gctcggttgt cgccacgcgc acgccgccga cgtagagcac gtaacggccg tcttcttctt
      901 cgtagcgctc catggcgcgt cagacgccag gttcgaagat ttgtcacatg caatcggttg
      961 agcggcctta tgctggcctc cgcgtggccg agaaggaagc cgatgcggag ctcgtgcgcc
     1021 tggcgcaggg cggagacgag cgcgctttcc aggcgttggt ggtcaagtac cagcgccgga
     1081 tcgcgcggca cgtggcgcgc tacgtacggc gcgcagccga cgtcgaagag gtggtgcagg
     1141 acgcattcat ccgcgcctac cgcgggcttg cgtcattccg cggcgagtcc agcttctaca
     1201 gctggctcta ccgcatcgcg accaatgcgg ccttcagctt cctgaagaag accgccggcg
     1261 aggtgcaggc cgacgaacgc tccgacgagg gcttcgagcc tggcctgacc gacgagcagt
     1321 cgcccgagcg catcttgctc gcccggcaga tcggcgacgc ggtggagcgt gccatggcgc
     1381 ggctgcagcc cgagctcgcc gaggcgctgg tgctcttcga ggtcgaaggc aagtcgtaca
     1441 aggaaatcgc gcagatgctc ggcacgccaa tcggcacggt gcgcacgcgt atcttccgcg
     1501 cgcgcgagtt catcgcgcgc aggctcgagc cggtgctcgg tcccgcgcgc gacaggaggt
     1561 ggtagccatg tcgctcaagg tatcgctgct gcttgacggc cagctcgaac ccgcggaggt
     1621 caagcccgcg ctggaggagc tcgctgcgga cgccgcgctg cgcgaccgct acactctcta
     1681 tgggcttgcg ggcgacgccc tgcggggcaa cccaacgccg gacgacggtt tctcgatccg
     1741 gattttcgag cgcatgaagc gcgaagcggt gaaaatcgag cccggcttcg atccgctgaa
     1801 agactaggcc gcagcgcgct cggcgcgctt gcgctcgtgc tccttgagga agcgcttgcg
     1861 caggcggatc gacttcggcg tgatctcggc gagctcgtcg tcgccgatga actccactgc
     1921 gtactcgagc gtcagcgcga tgggcggggt gagcagtatt gcgtcgtcct tgcccgcggc
     1981 gcggaagttg gtgagctgct tggcgcggat cgggttcacg acaaggtcgt tgtcgcggct
     2041 gtggatgccg atgatcatgc cctcgtagac cgggtcgccg gggctcacga acatgcggcc
     2101 tcgctcctgc agcttccaca gcgcatatgc gacggcggcg ccgtcgtcct gcgacacgag
     2161 cacgccgttc ctccgctcgg gaatctcgcc cttcaccggg gcgtagccgt cgaacacgtg
     2221 gctcatgatc ccggtaccgc gcgtgaggtt catgaactcg ccctggaagc cgattagccc
     2281 gcgcgccggg atgcagtagt ccaggcgcac gcggcctttg ccgtccggct gcatgtcgcg
     2341 cagctctccg cgccgccgtc ccagctcctc catgactgcg ccctgattct gctcttcgag
     2401 gtccactgtc aagagctcga agggttcgca cttctcgccg tcgatctcct tgcatacgac
     2461 ccgtggccgg gacaccgcga cctcgtagcc ctcacggcgc atgttctcga tcaggatggt
     2521 gaggtgcagc tcgccgcgcc cggacacgat gaaggtatcg gtgtcggcgg ggtattccac
     2581 gcgcagtgcc acattgcttt tcgtctcccg ctcgaggcgc tcgcgcagct ggcggctggt
     2641 gacaaagcgg ccctcgcgcc cggcgagcgg ggaagcattc accaggaaat tcatggtcag
     2701 cgtgggttcg tctaccttga gcggcggtag cgcctcggga tgctcggggt cgcatagcgt
     2761 cgcgccgatg ccgacctcgt cgatgccgtt gatgaggacg atatcgccgg cttccgcctc
     2821 ctcgacgggc ttgcgctcga ggccttcgaa tttcaggact tggttgactt tggtgagaag
     2881 cgaagcgccg ttctccccat tcatcaggaa aacctgctgc gccggccgta ttcgcccgcg
     2941 ccggatccgc ccgatgccga tcttgcctac gtaactcgag tagtcgagag agcagatctg
     3001 gagctgaagc ggggcgtccg ggttctcgtc gcgcaccggc acatgctcga ggatcgcgtc
     3061 gaaaagcggt tgcatattgg tgcccgtttc gccgggattg cgagccgacc agccttgcaa
     3121 ggcagatgca taaatgaccg ggaaatcgag ctggtcctcg gctgcgccga gcttgtcgaa
     3181 gagctcgaag gtctggttca cgacccagtc ggaacgcgcg ccgggacggt cgaccttgtt
     3241 gacgaccacg atgggcttga gcccctgggc gagcgccttg cgggtgacga agcgcgtctg
     3301 gggcatcggc ccttcgaccg cgtcgacgag gagcagcacc ccgtcgacca tggacagcac
     3361 gcgctcgacc tccccgccga agtccgcgtg gccgggagtg tcgacgatgt tgatgtgggt
     3421 gccgaggtag cggatggcgc agttcttggc gaggatcgtg atgccgcgct cgcgctcgag
     3481 ctcgttcgag tccatgaccc gctcctcgac ccgctcgtgt gcggcgaagg tgcccgactg
     3541 ctgcagcagc ttgtcgacga gcgtcgtctt gccgtggtcg acgtgggcga taatcgcgat
     3601 gttgcgccgg gcgcggttca tggggccgcg cattgtacgt taatggaagg ggatgacgga
     3661 ctcgtttctg ggtaaaattt ccgctcccca gtccccgtaa tccaacccga gatcgaacgc
     3721 atggacaccg cacttctatc caagaccgcg gccgcgatgg tcgcgaaggg caaaggaatt
     3781 ctcgccgccg acgagtcgag cggcacctgc gagagccgct tcaagtcggt cggcgtcgaa
     3841 tgcaccgagg agaaccgccg gacctaccgc ggacttctct tcactacgcc cggcgtcgag
     3901 cagtacatct cgggcgtgat cctgttcgac gagaccatcc gccagaaagc caacgacggt
     3961 gtgctgttcc cgcagtacct ggcgaagaaa ggcatcattc ccggcatcaa agtcgacgcc
     4021 ggcgcgaaga gcctgccgct gcacgccggc gagaaggtga ccgaaggcct cgacggcttg
     4081 ccgaagcgcc tggacgagta ctcgaagctc ggcgcccgct tcgccaagtg gcgcgccgtg
     4141 atcacgatcg gtgcgggcat tccgagccat gcctgcatct atgccaacgc ccacgcgctc
     4201 gcgcgttacg ctgccgcctg ccaggaagcc tcaatcgtgc cgatcgtcga gcccgaggtg
     4261 ctgctcgacg gcgaccacac gatcgagcgt tccgaggaag tgaccgaggc aaccctgcgc
     4321 gcgtgctatg cggcacactc tgcctacaac gtcgtgcccg aatacgtgat cctcaaggca
     4381 agcatggtgg ttccaggcaa gagctgcccg aaccaggcga gcgtggagga agtcgccgag
     4441 cgcacggtgc gcgtgctcaa gcgcacggtc ccggcctcgc aacccggcgt tgtgttcctc
     4501 tcaggcggcc agtccgacga ggccgccacc gcgcatctca atgccatggc gtcgatgaag
     4561 gggttgccct ggcctctgac cttctcgtac tcgcgcgcgc tgcagaaccc ggcgctcaag
     4621 acctggaaag gccaggcggg caacattgcc gccgcgcaga aggctttctt ccaccgcgcg
     4681 cacatgaacg gactcgccgc gcagggcaag tggcgcgccg agctcgagaa gcaggcggcg
     4741 tagtgggcgc tttgttcgag tcgaacatca agagcctgcc tcgcatcggg aaggggaagg
     4801 tgcgcgacat ctacgccgtc ggccaggaca agatgctcat cgtggctagc gatcgcctct
     4861 cggcgtttga cgtcgttctg cccgacccga tcccggacaa gggccgggtg ctgaacgaga
     4921 tggcgaattt ctggttcggt cggctctgcc acgtcgtggc aaaccagctg accggaatag
     4981 atcctgaatc tgttgtatct gaagaagaga aagcgcaggt gcgcggccgc tcggtcgtgg
     5041 taaagaagct gaggcccttg ccgatcgagg cggtagtgcg cggctacatc attggctcgg
     5101 gctggaagga ctaccagaag accgggaaga tctgcggcat cgagctgccg aaggggctgc
     5161 gccaggccga gaagctgccc cagccgattt tcacgccggc caccaaggcc gcggaaggcc
     5221 acgacgagaa catcaccttt gcgcaggtcg agaagctcat cggcctggag ctcgctgcca
     5281 aggtgcgaga cgtgagcatt cgcctctaca aggaagccgc cgattatgcg gttaccaagg
     5341 gcatcatcat cgccgacacg aagttcgagt ttgggttgga tgaacacaac aatctggtgt
     5401 tgatcgacga ggtattaact gccgactcgt cccgtttctg gccagccgat tcctaccgcg
     5461 tgggcatctc gccgccttcg tatgacaagc agtacgtgcg cgactatctg gaatcgctca
     5521 cctgggacaa gactccgccg gcgccgacgc tgccggaaga cgtcattgcg cgcacgtcgc
     5581 agaaatatcg cgatgccctc gaacggctca cggggaggaa gctcccttga tcggcatcgt
     5641 gatgggctcg acctccgatt gggaaaccat gaaggcggcg gccaaggttc tggaagagtt
     5701 cgccgttccg tacgaggcga aggcgatgtc ggcgcaccgc acgccgcacg cggtcgcgga
     5761 gtgggcgacg aatgcgcaaa agaagggttt caaggcgatc atcgccgccg ccgggggtgc
     5821 ggcgcatctt gcgggcgtca tcgcggcgca taccacgctg ccggtgctcg gtgtgccgat
     5881 gccttccaag catctgcagg gactcgactc gcttctctcc acagtccaga tgccgcgcgg
     5941 catcccggtc gcgaccttcg ccatcggcga agccggcgct gccaacgcgg ctctttttgc
     6001 ggtggcattg cttgcgcttg cggacaagca gattgcagga aagctggaag actttcgccg
     6061 caagcagacc gaagcggtgc tgaagagccc gccgctcaag ctgtgaagcc cgaaatccgc
     6121 cgggcggcgc agatcctgcg cgccggcgga ctggtcgcgt tcccgaccga aaccgtttac
     6181 gggctgggcg cggacgcgac gaacgctgaa gctgtccgga agctctacgc cgtcaagcgc
     6241 cgccccgccg accatcccgt gatcattcat tttgcggatg ccgaaacggc cttcacgtgg
     6301 gcccgcgagg tgcccgaggg cgcgcgcgag cttgccggga ggttctggcc gggtccgctg
     6361 acgctgatcc tcaaacgctc gccgctcgcc aaggacttcg tcaccggtag ccaggacacc
     6421 gtcggcctgc gcgtgccatc gcatcctgtt gcacaggagc ttctcaagga attctcagga
     6481 gcgattgctg ccccgtcagc caaccgcttc gggctggtgt cgcccaccac cgccgcgcat
     6541 gtgcgcgacg atcttggaag cgaggtcgag cttgtgctcg aaggcggcgc gagcgaggtc
     6601 gggatcgaat cgacgatcct cgatctttcc gggccgagcc cagtgctgct gcgtccgggg
     6661 catattgcca aggatcagtt gcgtgaggtg ctcgctttgg aggtattcga aaaagcgctt
     6721 gacgcgcccc gccacccggg cggcatggaa cgccattacg cgccacgcac gccggctcag
     6781 cttgtcttca cttatgaact ggacaagacg atcgcccagg caaagggaga cgttgcggtg
     6841 ctcgccttct cgcgcccgga cgagcgggtg gactactggc tgcgcatgcc gcgagacccc
     6901 aagacgtacg cgcagcgcct gtacgcagcg ttgcgcgaac tggacacggc gggctgcgag
     6961 cgcatcatta tcgaggcacc gcccttgtcg ccggaatgga gcgcggtgcg cgaccggctg
     7021 aagcgcgctt gtggacgggc gtaagatcgc agcgctgaga ggctttctcc tgggtgtcct
     7081 gctgctcgcc gggtgtagtt cgaacacggc atggcggctg agcgccggcg cgccg
//
LOCUS       DASTPD010000049         6883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_22166, whole genome shotgun sequence.
ACCESSION   DASTPD010000049 DASTPD010000000
VERSION     DASTPD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22166"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFR57_01960"
     CDS             complement(<1..352)
                     /locus_tag="VFR57_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0258167.1"
                     /translation="MSLHLEHLNPEQLAAVTLPDEHALILAGAGSGKTRVLTTRIAWL
                     IKGGRVVPAGILAVTFTNKAAREMLTRVAAMMTVNPRGMWIGTFHGLCNRMLRAHARE
                     AGLPQAFQILDSQDQ"
     gene            complement(362..1315)
                     /locus_tag="VFR57_01965"
     CDS             complement(362..1315)
                     /locus_tag="VFR57_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258168.1"
                     /translation="MKKVLLLAVLAFAAGAQAQTWPSKAVRFIVPFPPGGSTDVAART
                     VADKLTRALGAQVLVENRAGGGGAIGTVEAARAAPDGYTLLFAADPVITLHLVVKNMQ
                     FDMQRDFIAVTQVTTQPIAVAVHSSLPVKDVQKLIAYAKANPGKLSFAHSGTGSGQHM
                     SGELLKKMAGIDMVHVPYKGGGPAVQDLVGGQVPVGVLGSTPLIPHHKAGRIRIIAFT
                     SKERFPPMPEIPTLHESGLVGFDTTQWLGILAPKGTPAEIVDRVYAETRKALELQDVK
                     EKLAQAALQPVGNSPKEFEALIRADLERWTAVAKELKLEPQ"
     gene            1351..3909
                     /gene="leuS"
                     /locus_tag="VFR57_01970"
     CDS             1351..3909
                     /gene="leuS"
                     /locus_tag="VFR57_01970"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU0258169.1"
                     /translation="MQEAYQPASVEREAQAFWANRDSFRSTEERARPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYYRMRGYNVLQPMGWDAFGLPAENAAMANKVPPARWTY
                     DNIAYMKSQLQSLGFALDWKRELATCKPEYYRWNQWLFTRLFRKELAYKKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGALVEKREIPMYFLRITAYAEELLAALDGLPGWPEQVKL
                     MQKNWIGRSAGVRIGFPYRLESQDRVLWVFTTRADTLMGATFMAVAAEHPIALHAARR
                     NHELGRFVDECRKGAVIEADLAQIEKRGMPTGLFVSHPLTNEKIPVWVGNYVLMGYGE
                     SAVMGVPAHDERDYEFALNHKLPIKPVIRHPLGDTVPAPWKPEYAEYGVCINSGPYDG
                     LGYQEAVDHIAEDLRVKGLGEKQVQWRLRDWGISRQRYWGTPIPIVHCAACGDVPVPD
                     AELPVVLPEDVVPDGTGNPLAKKPEFYQTKCPQCSRPANRETDTMDTFVDSSWYFARF
                     ACPDQNKAMLDERANYWMPVDQYIGGIEHAILHLLYSRFFQRVMQEETGLMPGVPEPF
                     TNLLTQGMVLAESYYTDASGRREWVNPAEVEVQRDSKGNIASAKRASDGVPVVYDGIG
                     TMSKSKNNGVDPQALIEQHGADTARFYTIFASPPTNTLEWSDKNVAGSARFLKSVWTF
                     AYRFSRATKSKDVDKKIRFRIHADLKQANFDMQRHQFNTVASACMKILNALEELPEWN
                     ATSEEGLSILLRLLSPIAPHIAHHVWRELGFGEDVMTAPWPEPDPAALEQDEIELVVQ
                     VNGKLRGSIRVPKAADRAAIEELARANPNVQRFVAGQNVKKIVVVPGRLVNLVV"
     gene            3899..4399
                     /gene="lptE"
                     /locus_tag="VFR57_01975"
     CDS             3899..4399
                     /gene="lptE"
                     /locus_tag="VFR57_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEU0258170.1"
                     /translation="MSSRALLVALALAVASCGFHLRGTAELPFRSLYVPGANTGIGLD
                     LKRNIQAGTGAKVVDTPATADAVLEFTQEAREKEILSLTSTGRVREFQLRYRVGFRVH
                     DGKGANYVPPSVVQLTRDVTYNDAEILAKEAEEQLLFRDMQADMVQQIMRRLAAAKPP
                     KPAATQ"
     gene            4396..5352
                     /gene="holA"
                     /locus_tag="VFR57_01980"
     CDS             4396..5352
                     /gene="holA"
                     /locus_tag="VFR57_01980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU0258171.1"
                     /translation="MRLRGEQLAGHLAKTLAPVYGVLGDEPLLLLEAADAVRAAARKQ
                     GYTEREVYEPGRSFDWSELAHAGASLSLFGSRKMVELRLASGKPAPQASTAIVAWCER
                     PNPEALLLVTMPRPEGGGWWEAPWFLALNQAGIIVEIQSVSRAALPGWLEKRLARQKQ
                     SASREVLQYLADRIEGNLPAAHQEVQKLALLAPEGELSLETVRDAVADVARYDTYVAA
                     DALVSGDTARYLRVLEGLRGEGEAPTYLLFTLSSALFALATGERVTKAMQAVLDKARR
                     YPPAAIQRAIRRAAAVDRAIKGVDAVEPWEEFAKLGLELHAS"
     gene            5475..6758
                     /locus_tag="VFR57_01985"
     CDS             5475..6758
                     /locus_tag="VFR57_01985"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU0258172.1"
                     /translation="MQFTRNKHAHGDLYAMMEEMGRAARAAARALARASTEAKNSALA
                     AMAAGLRSESASIVQANAEDARKAKKAGCDTAFVDRLTLTPALVEQMAAGVEQVAALP
                     DPVGAISDRVKRPSGLEVAKMRVPIGVVAIIYESRPNVTADAAALCVKAGNACILRGG
                     SEALESNRAIAQCVRAGLKAAGLPETAVQLVGTTDRAAVGALITMREHIDIIVPRGGK
                     QLIERLARESRIPMIKHLDGVCHVYIDDSADPEMAVRIADNAKTQRYGTCNTMETLLV
                     AEGIASAVLPRIGEIFSSKNVEMRADAAALKYVKGRKATENDYYTEWLAPVVSIRVVK
                     GLDEAIEHISKYGSQHTDAIVAGDRTRAERFLRQVDSSSVMWNASTRFADGYEYGLGA
                     EIGISTDKLHARGPVGLEGLTTQKYIVLGHGEIRT"
BASE COUNT         1254 a         2258 c         2262 g         1109 t
ORIGIN      
        1 gctggtcctg cgagtcgagg atctggaacg cctgggggag gccggcctcg cgcgcatggg
       61 cacgcagcat gcggttgcac agaccatgga aggtgccaat ccacatgccg cgcgggttga
      121 ccgtcatcat ggcggcgacg cgcgtcagca tctcgcgcgc cgccttgttg gtgaaggtca
      181 ccgcgaggat tcccgccggc acgacacgcc cccctttgat gagccaggcg atgcgcgtcg
      241 tgagtacccg cgtcttgccg ctgcccgcgc cggcgaggat taacgcgtgc tcatccggca
      301 gggtgaccgc ggcgagctgc tcggggttga ggtgttcgag gtgaagcgac aagccaccat
      361 tttactgagg ctcgagcttc aattccttcg caactgcggt ccagcgctcc agatccgcgc
      421 gaatcagcgc ctcgaattcc ttcggcgagt tgccgaccgg ctgcagcgcg gcttgcgcga
      481 gcttttcctt cacatcctga agctcgagtg ctttgcgggt ctctgcgtaa acccggtcca
      541 cgatctcggc cggcgtgccc ttcggcgcca ggatgccgag ccattgcgtg gtgtcgaagc
      601 cgaccaggcc cgactcgtgc agcgtgggga tctcgggcat cggcgggaag cgctccttgg
      661 aggtgaaggc gatgatgcgg atgcggcccg ccttgtggtg cgggatgagc ggcgtcgagc
      721 ccagcacacc gaccggcacc tggccgccga cgagatcctg gaccgcgggc ccaccgccct
      781 tatacggcac gtgcaccatg tcgatcccgg ccatcttctt aaggagctcg cctgacatgt
      841 gctggccgct gccggtgccc gagtgcgcga acgacagctt gccggggttc gctttggcgt
      901 aggcgatgag cttctggaca tccttcaccg gcagcgagct atgtacggcc accgctatcg
      961 gctgcgtggt cacctgcgtc accgcgatga agtcgcgctg catgtcgaac tgcatgttct
     1021 tcacgacaag gtgcaacgta atgaccggat cggctgcgaa aagcagcgtg tagccgtcgg
     1081 gtgcggcgcg cgccgcttcc accgtgccga tggccccgcc gccgccggcg cggttctcga
     1141 ccaagacctg cgcgccgagc gcgcgggtca gcttgtcggc gaccgtgcgc gcggcaacgt
     1201 cggtcgagcc gccgggcggg aaaggcacga tgaagcgcac agccttcgat ggccaggttt
     1261 gcgcctgggc gcccgctgcg aaagcaagga ctgcaagaag aaggaccttc ttcatggatg
     1321 cgataatccg ccgcttcggc ggaaccttac atgcaagagg cttaccaacc ggcaagcgtg
     1381 gagcgcgagg cccaggcctt ctgggcaaac cgcgattcct ttcgctcgac cgaagagcgc
     1441 gccaggccga aattctactg tctctccatg ttcccctacc cctcggggaa gctgcacatg
     1501 gggcacgtgc gcaactacac gatcggcgat gtgctcacgc gctactaccg catgcgcggc
     1561 tacaacgtgc tgcagccgat gggctgggac gcgttcggcc tgccggccga gaacgccgcc
     1621 atggcaaaca aggtcccgcc cgcgcgatgg acgtacgaca acatcgccta catgaagtcg
     1681 cagctacaga gcttgggctt cgcgctcgac tggaagcgcg agctcgccac ctgcaagccc
     1741 gagtactacc gctggaacca gtggctcttc acacggcttt tccgcaagga gctcgcctac
     1801 aagaagaccg gcgtcgtcaa ttgggatccc gtcgaccaga cggtcctcgc caacgagcag
     1861 gtgatcgagg gccgcggctg gcgcaccggg gcgctcgtgg aaaagcgcga aatcccgatg
     1921 tattttctgc gcatcacggc ctatgccgag gagctgctcg ccgcgctcga cgggctgccc
     1981 ggctggccgg agcaggtgaa gctcatgcag aagaactgga tcgggcgctc ggcaggcgtg
     2041 cgtatcggct ttccctatcg gctggaaagc caggaccggg tgctatgggt gttcacgacc
     2101 cgcgcggaca ccttgatggg cgcgaccttc atggcggtcg ccgccgagca cccgatcgcc
     2161 ctgcacgcgg ctcgacgaaa tcatgagctg ggcaggttcg tggacgagtg cagaaaaggc
     2221 gcggtcatag aagccgacct cgcccagatc gaaaagagag gaatgccgac gggcctcttt
     2281 gtatcccacc cgctgaccaa cgagaagatc ccggtatggg tcggcaacta cgtcctgatg
     2341 ggatacggcg aaagcgcggt catgggcgta ccggcgcacg acgagcggga ctacgagttc
     2401 gcgctaaacc acaagctgcc gatcaagccg gtcatccgcc accccctcgg cgacaccgtg
     2461 ccggcgccct ggaagcccga gtacgccgag tatggcgtgt gcatcaactc cgggccgtac
     2521 gatggcctgg gctaccagga agcggttgat cacattgccg aggatctcag ggtaaagggc
     2581 cttggcgaga agcaggtgca atggcgcctg cgcgactggg ggatttcgcg ccagcggtac
     2641 tggggcacac ccatcccaat cgtccactgc gccgcctgtg gcgacgtacc ggtgccggac
     2701 gccgagcttc cggtcgtact ccccgaagac gtggtacccg acggcaccgg caatccactc
     2761 gccaagaagc ctgagttcta ccagacaaag tgtccgcagt gctcgcggcc tgcgaatcgc
     2821 gagaccgaca cgatggatac gttcgtcgac tcttcgtggt acttcgcgcg cttcgcttgt
     2881 ccggaccaga acaaggcgat gctcgacgag cgggcgaact actggatgcc ggtcgaccag
     2941 tacatcggcg gcatcgagca cgctatcctg catctcctgt actcgcgctt cttccagcgc
     3001 gtcatgcagg aagagaccgg cctcatgcca ggggtgcccg agcctttcac caacctgctg
     3061 acccagggca tggtgctcgc cgagtcctac tacaccgacg cgagtggccg gcgggaatgg
     3121 gtgaatcccg ccgaggtcga agtgcaaagg gattccaagg gcaacatcgc ttcggcgaag
     3181 cgcgcttcgg atggcgtccc cgtcgtgtac gacgggatcg gcacgatgtc caagtcgaag
     3241 aacaacggcg tcgaccccca ggcgctgatc gagcagcatg gcgcagacac cgcgcgtttc
     3301 tacaccatct tcgcctcgcc tccgaccaac acgctcgagt ggagcgacaa gaatgtcgcc
     3361 ggctcggcga ggttcctgaa gtcggtatgg acatttgcct acaggttctc cagggcgacc
     3421 aagagcaagg acgtcgacaa gaagatccgc ttccgtattc acgcggacct gaagcaagcg
     3481 aacttcgaca tgcagaggca ccagttcaac accgtcgcct cggcctgcat gaagatcctc
     3541 aacgcactcg aagagctgcc cgagtggaac gcgaccagcg aggagggcct ctccattctg
     3601 ctacggctgc tttcgccgat cgccccgcac attgctcacc atgtgtggcg cgagctcgga
     3661 ttcggcgagg acgtgatgac cgccccgtgg cccgagcccg accccgcggc gctggagcag
     3721 gacgagatcg agctggtggt gcaggtgaac ggcaagctgc gcgggtcgat ccgcgtgcct
     3781 aaggctgccg accgcgcggc gatcgaggag ctggcgcgcg cgaaccccaa cgtgcagcgc
     3841 ttcgtcgccg ggcagaatgt gaagaagatc gtcgtcgtgc ccggacggtt ggtgaacctt
     3901 gtcgtctagg gccctcctcg tcgcgttggc gctcgccgtc gctagctgcg ggttccacct
     3961 gcgcggcacg gccgagctgc cgtttcggtc gctctacgta cccggcgcga acaccgggat
     4021 cgggctcgat ctcaagcgca atatccaggc tggcaccggg gcgaaagtcg tggacactcc
     4081 ggccaccgcc gacgcggtgc tggaattcac gcaggaggcg cgggagaagg agatcctgtc
     4141 gctcaccagc accggccggg tgcgagagtt tcagctgcgc tatcgcgtag gcttccgcgt
     4201 gcacgacggc aagggtgcga attacgtgcc gccgagcgtg gtgcagctca cgcgcgatgt
     4261 cacctacaac gacgcagaga tcctggcgaa ggaagcggag gagcagctcc ttttccgcga
     4321 catgcaggcc gacatggtgc agcagatcat gcgccggctc gccgcggcga agccgcccaa
     4381 gcccgcggcg acgcaatgag gctacgcggg gagcagctcg ccggtcatct tgcgaagacc
     4441 cttgccccgg tttacggcgt cctcggtgac gagccgctcc tcctgctcga ggctgccgac
     4501 gcggtgcgcg ccgcggcccg caagcagggc tacaccgagc gcgaggtcta cgaaccggga
     4561 cgctccttcg actggagcga gcttgcgcac gccggggcga gcctttcgct cttcggcagc
     4621 cgcaagatgg ttgagctcag gctcgcgagc gggaagccgg cccctcaggc ctcaaccgcg
     4681 atcgtcgcct ggtgcgagcg cccgaacccg gaagcgctgc tcctagttac catgccacgc
     4741 cccgaaggcg gcgggtggtg ggaagcgccg tggtttctgg cgcttaacca ggcagggatc
     4801 atcgtcgaaa ttcagtcggt gtcgcgcgct gcgcttcccg gctggctgga aaagcggctc
     4861 gcgcgccaga agcagagcgc ctcgcgcgag gtgctgcagt atctcgccga ccgcatcgaa
     4921 ggcaatctgc ctgccgcgca ccaggaggtg cagaagctgg cgctgcttgc gccggaaggc
     4981 gagctctcgc tcgaaacggt gcgtgatgcg gtcgccgacg ttgcgcgcta cgacacatat
     5041 gtcgccgcgg acgcactcgt ttccggcgat accgcgcgct accttcgggt gctcgaaggc
     5101 ctgcgcggcg aaggcgaggc gccaacttac cttctcttca cgctgtcgag cgcgctcttc
     5161 gcgctcgcca cgggcgagcg cgtgaccaaa gccatgcagg cggtgctcga caaggctcgc
     5221 cgctacccgc ccgctgcgat ccagcgggcg atccgccgcg cggccgcagt ggaccgcgcg
     5281 atcaagggtg tggacgcggt cgagccgtgg gaggagttcg cgaagctggg gctcgagctc
     5341 cacgcctcct gattcgcttc tcgcgctcga gcagaatcac gcgcagcccc gcgtcgccga
     5401 gcgcaacagc ccacgcaagc ccgccaagcc ctctaccggc gatcacagca tgcttagcca
     5461 ttacaatggt gtgcatgcag ttcacgcgca acaaacatgc ccatggcgac ctgtacgcca
     5521 tgatggagga gatgggccgc gcggcccgcg ccgcggcccg cgcgctcgcg cgcgcgagca
     5581 ccgaggcaaa gaactccgcc ctggctgcca tggccgccgg ccttcgttcc gagtcggcca
     5641 gcatcgtgca ggctaacgcg gaagatgcaa gaaaagcgaa gaaagccgga tgcgacacgg
     5701 cgttcgtcga ccggctgacg cttacgccgg cgctcgtcga acagatggcc gcaggcgtcg
     5761 agcaggtggc agcgctgccg gatccggtcg gcgccatttc cgatcgagtc aagcgcccga
     5821 gcggcctcga ggtggcgaag atgcgggtgc cgatcggcgt ggtggccatc atctacgagt
     5881 cgcgtccgaa cgtcaccgcg gacgcagcgg cgctttgcgt gaaggcgggc aacgcgtgca
     5941 tcttgcgcgg cggctctgag gcgctggaat cgaaccgcgc aatcgcgcag tgcgtgcgcg
     6001 ctgggctcaa ggcggccggg cttcccgaga cggcggtgca gcttgtcggc accaccgacc
     6061 gcgccgcggt gggcgcgctg atcaccatgc gcgagcatat cgacatcatc gtgcctcgcg
     6121 gcggcaagca gctgatcgag cgcctggcgc gcgagtcgcg catccctatg atcaagcatc
     6181 tcgacggcgt gtgccacgtc tacatcgacg acagcgccga tcccgagatg gcggtgcgca
     6241 tcgccgacaa cgcaaagacc cagcgctacg gcacctgcaa cactatggaa acgctgctcg
     6301 tggcagaggg gattgcgtcg gccgtactgc ctcgcatagg agagattttt tcatcgaaga
     6361 acgtcgaaat gcgcgccgac gccgccgcct tgaagtacgt caaaggcagg aaagccacgg
     6421 agaacgacta ctacaccgaa tggcttgcac ccgtcgtctc catccgcgtg gtaaagggcc
     6481 tcgacgaagc gatcgagcac atctcgaagt acggctcgca gcacaccgac gccatcgtcg
     6541 ccggcgaccg gacgcgcgcc gagcggtttc tgcgccaagt cgactcgagc tcggtcatgt
     6601 ggaacgcctc cacgcgtttc gccgacggtt acgagtacgg attgggcgcc gagatcggca
     6661 tctctaccga caagctgcac gcgcgcggtc cggtcggcct cgagggcctg accacccaga
     6721 agtacatcgt cctcggccac ggcgagatcc gcacctgatg gagaggagtt gaaggcaggc
     6781 attctgggtg gcaccttcga cccggtacac aacgcgcacc tcgcgatggc ccgagccgcg
     6841 cttggtcact tgaagctcga gaagatcatt ttcatgccca cgg
//
LOCUS       DASTPD010000050         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_635317, whole genome shotgun sequence.
ACCESSION   DASTPD010000050 DASTPD010000000
VERSION     DASTPD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635317"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1041
                     /locus_tag="VFR57_01990"
     CDS             262..1041
                     /locus_tag="VFR57_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0258173.1"
                     /translation="MSDLSLPKIQSMRSRVSPAEWETRVDLAACYRLVHHYGMDDLVY
                     NHISARVPGDEGHFLINAYGMMYDEVTASSLVKIDFDGNVVLDSGTGYGINHAGFVIH
                     SAVHKGRPDVGCVIHTHSIAGMAVSALDCGLLPITQTAMYFGGVGMHEYESVAIDLAE
                     QERLVRDLGNHKAMILRNHGLLAVGPTVSEAFTNLFWLERACKAQVAAMACNTKLHLP
                     AQDVIDKTAHLYDPKTRRRWGPLEWPALLRLADRKYPGFRD"
     gene            1038..1943
                     /locus_tag="VFR57_01995"
     CDS             1038..1943
                     /locus_tag="VFR57_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258174.1"
                     /translation="MKSLQDKSVVVTGAGGGIGRDFAHAMAAHGAKVLVNDLGTTVKG
                     EGRDAGPAQKVVDEIRAKGGTAVANTDSVADWESANRIISSAVDAFGRIDVVVNNAGI
                     LRDRFFFNMSVEEWRAVIDVHLNGSFYVSRAAAPYFKNQNSGCYVHMTSTSGLVGNLG
                     QANYSAAKLGIVGLSKSIALDMAKYNVRSNCISPFAWSRMIGSIPTETDEQRARVEKL
                     KAMETAKIAPLAVYLASDTDVTGQIFAVRANEIFLMSQSRPLRSVHRAEGWTAETIAA
                     HAMPAFRPHFYPLERSQDVFSWDPL"
     gene            1928..>2633
                     /locus_tag="VFR57_02000"
     CDS             1928..>2633
                     /locus_tag="VFR57_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258175.1"
                     /translation="MGPALKRVLLTDAIAPAGEEALSRAVEVVHAPDSAPATIRRLAG
                     DFDGILIRSKLPDDIFDAAPRVRAVVIHGTGTDLVPVESAAAHGVTVANLPGINAHSA
                     AEYCAMAMLILARNIVPITNGLRSLSWDEARRLGAPARELAALTLGIVGVGAIGSRLA
                     KIARNGFGMRVLGHQRRLERLPGEAEAAPLEDLLRQSDFVVVACPLTPQTRYLINPET
                     IAKMKRTAWLINVGRGP"
BASE COUNT          479 a          907 c          811 g          436 t
ORIGIN      
        1 ccctggagct cgggatttgc gacgagaagc accgcccgat ccaattcctg cgatcgagcc
       61 gtgcaggccg ccaagagcag gcccgccaat aagatctttc taagcgcgtc catgttcacc
      121 ccctgtgatg gcgcgcagcc ctgtagcact ttgcgcccgc gctgcaacga ccgctatgaa
      181 aaactgcccc ctcgcgcggt aagcggtgca gcaaagttgg cgaagtcact cagcgggagt
      241 gacccaccgc ataatagagg gatgagcgat ctgtccctcc caaaaatcca gtcgatgcgc
      301 agccgcgtct cgcccgccga atgggaaacg cgcgtcgatc tcgccgcctg ctaccgcctc
      361 gtccaccact acgggatgga cgaccttgtc tacaaccaca tttcggcgcg cgtgccgggc
      421 gacgaaggcc atttcctgat caacgcctac ggcatgatgt acgacgaggt caccgcctcc
      481 agcctggtga agatcgactt cgacggcaac gtcgtgctcg actccggtac cggctacggg
      541 atcaaccacg cggggttcgt gatccatagc gccgtgcaca aaggacgccc cgacgtgggc
      601 tgcgtgatcc acacgcattc gatcgccggc atggcggtct cggcgctgga ctgcgggctt
      661 ttgccgatca cgcagaccgc gatgtacttc ggcggcgtcg gcatgcacga gtacgagagc
      721 gtggcgatcg acctcgccga gcaggaacgg ctggtgcgcg acctcggcaa tcacaaggcc
      781 atgatcctgc gcaaccacgg gctgctcgcg gtcggcccga cggtctcgga agccttcacg
      841 aaccttttct ggctcgagcg cgcgtgcaag gcgcaggtgg ccgcgatggc gtgcaacacc
      901 aagctgcatc ttcccgctca ggacgtgatc gacaagacgg cgcacctcta cgacccgaaa
      961 acgcgccggc gctggggccc gctcgagtgg ccagcgctcc ttcgcctcgc cgacaggaag
     1021 tatccaggct tccgcgattg aagtcccttc aggataagag cgtcgtcgtc accggcgcgg
     1081 gcggcggcat cggccgcgat ttcgcccatg cgatggcggc ccacggcgcg aaagtgctcg
     1141 tcaatgacct cggcaccaca gtcaaaggcg aaggccggga tgcgggccct gcacagaagg
     1201 tcgtcgacga aatccgcgcg aagggcggga ccgcggtcgc gaataccgac agcgtggctg
     1261 actgggagtc cgcaaaccgg atcatctcct ccgccgtcga tgcgttcggg cgcatcgacg
     1321 tggtcgtcaa caacgccggc atcctgcgcg accgcttctt cttcaatatg tcggtcgagg
     1381 agtggcgtgc cgtgatcgac gtgcatctca acggctcgtt ctacgtctcg cgcgccgccg
     1441 cgccgtattt taaaaatcag aattcagggt gctacgttca catgacctcg acctccggcc
     1501 tcgtcggcaa cctcggccag gcaaactact ccgcggccaa gctcggcatc gtcggcctct
     1561 ccaaatcgat cgcgctcgac atggcaaagt acaacgtgcg ctcgaactgc atctcgcctt
     1621 tcgcgtggag ccgcatgatc ggttccatcc cgaccgaaac ggacgagcag cgcgcgcgtg
     1681 tcgagaagct gaaagccatg gagaccgcca agattgcgcc gcttgcggtg tacctcgcct
     1741 ccgacaccga cgtgaccggc cagatcttcg ccgtacgcgc gaacgaaatc ttcctcatgt
     1801 cgcagagccg tccgctgcgc tcggtgcacc gcgcggaagg ttggaccgcc gagaccatcg
     1861 ccgcgcacgc gatgccggcg ttccgaccgc acttctatcc cctcgagcgc tcgcaggacg
     1921 tgttctcgtg ggacccgctt tgaagcgggt ccttctcacc gacgccatcg cgcccgctgg
     1981 agaagaagcg ctgtccagag cggtggaggt cgtgcacgca cccgacagcg cgcccgccac
     2041 gatccggcgc ctcgcgggcg acttcgacgg catcctgatc cgcagcaagc tgccggatga
     2101 catcttcgac gcggcgccgc gcgtgcgcgc ggtggtgatc catggcaccg gcaccgatct
     2161 ggtgccggtg gaaagcgcag ccgcccacgg cgtaaccgtg gccaatctgc cgggcatcaa
     2221 cgcgcattcc gccgccgagt actgcgcgat ggcgatgttg atactcgctc gaaacatcgt
     2281 tcccatcacg aatggcctgc gttcgctttc ctgggacgaa gctcgccgcc tcggcgcccc
     2341 ggcgcgcgag ctcgccgccc ttacgctcgg catcgtcggc gtgggcgcga tcggcagccg
     2401 gctcgcgaaa atagcgcgca acgggttcgg gatgcgcgtt ctcggccacc agcgccggtt
     2461 ggagcgcctc ccgggcgaag ccgaagcggc acctcttgag gacctgctgc ggcaaagtga
     2521 cttcgtcgtc gtcgcctgcc cgctgacccc gcagacgcgc tacctcatca acccggaaac
     2581 gatcgcgaag atgaagcgaa ccgcgtggct catcaacgtt ggtcgcggcc ccg
//
LOCUS       DASTPD010000051        17455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_227958, whole genome shotgun sequence.
ACCESSION   DASTPD010000051 DASTPD010000000
VERSION     DASTPD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17455
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227958"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFR57_02005"
     CDS             <1..430
                     /locus_tag="VFR57_02005"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEU0258176.1"
                     /translation="ELEKLADVAYHPKDIAATISSGSYLTEGMVIAPCSMKTLAAVAH
                     AHADELVSRAADVVLKERRRLVLLPRETPLNLAHLRNMVAVTEMGGIVFPPVPAFYAA
                     PKSLDDLVAHTCARVLDLFGVHSPRLARWQGMKGIPEERG"
     gene            complement(378..1169)
                     /gene="pcaD"
                     /locus_tag="VFR57_02010"
     CDS             complement(378..1169)
                     /gene="pcaD"
                     /locus_tag="VFR57_02010"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEU0258177.1"
                     /translation="MKVKVNGIEIHYELHGKDGAPWLVLSHSLACSLRMWDPQIEAFK
                     GSHRILAYDMRGHGLTSAPAGPYTFDVLADDVLGLAASLKVTSAVYCGLSIGGMIGQT
                     IAIRGRGPFERMVLADTTHTQPPEAVTQWEERIRIAETKGMAALVDSTLERWFTPPFR
                     SSAAARKIAEQIAATPVAGYVGCGRAIMGLNTTAKLKGIKLPVLAITGEQDAAAPGTR
                     YIAENVPGAKLVVIPQASHIANIEQPEAFNRALREFLSSPASARA"
     gene            complement(1166..2308)
                     /locus_tag="VFR57_02015"
     CDS             complement(1166..2308)
                     /locus_tag="VFR57_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0258178.1"
                     /translation="MRPDYSGGSLVNLIASIVAARGGAPLHPALANLPASELGDARNI
                     VLLIVDGLGDNYLLRRGAGSALAGRRRGSLTSVFPSTTASAITTSYTGRTPLEHGLTG
                     WFTYFGEAGCVSAALPFRSRGDYLPLARRGVTPEQIYVCDSIFESVTDASFVITYKDI
                     IDSEYNIRHCRGAQRIAYESLDELVAGVEAAVKSADTRKFIYAYWPVYDMVSHRYGAE
                     SAEAFEQFTKIDAAFGEMVARLAGTESLIIATADHGFIDVAPEESLELPASLAALLRF
                     PLCGERRVAYCHVHDAPLFIQKARDWLGERAEVMPSRALVDEGWFGPGAPHPRFAERI
                     GDVALVMRGRYAVKDWTPGESRHLHIGNHGGTSEDEMLIPLIMEAT"
     gene            2352..2906
                     /locus_tag="VFR57_02020"
     CDS             2352..2906
                     /locus_tag="VFR57_02020"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEU0258179.1"
                     /translation="MRHEVHLHADIPILEGVSRRQLEQAFAPLLEYLDADGLADVKSL
                     EPDEPGIKYDEKELILTVCWTGEIGRSFHGALEATLESLGPYCYEAVEVDLTLYHENG
                     EQESKLLFVGPTAHAIHEAQRRCMVEDVAAILARQFGKEQVAQVTALVNELFDRDWKE
                     KASQQKKPERAFENYTRPSRKNLH"
     gene            2960..4252
                     /locus_tag="VFR57_02025"
     CDS             2960..4252
                     /locus_tag="VFR57_02025"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEU0258180.1"
                     /translation="MAGPKYPSLDTLALHAGQSPDPATGSRAVPIYQTTSYVFRDTEH
                     AASLFNMERAGHVYSRISNPTVAVLEERVAALEGGVGAIATASGQAALHLAIATLMGA
                     GGHIVASTALYGGSHNLLAYTLKRFGIETTFVSPRSLSQWKEAIGPQTRLLFGETLGN
                     PGLDVLDIPSLAQIAHAAKVPLLVDATFTTPVLMKPFALGADLLYHSATKFLGGHGVA
                     IAGVLVDSGRFDWEASGKFETLTAPYAGFHGMDFEEESGTRAFLLRARREGLRDFGAC
                     MAPMTAFQILQGIETLHLRMPRHVENTRKVVAFLSKNPAVEKVIYPELPDHPDHELAR
                     RLLPHGCGAVFTFEIKGGRPAGKRFIESLRLFSHLANVGDAKSLVIHPASTTHYRMSD
                     EDLRKAGITAGTVRLSIGLEDPQDLIEDLERALHASAK"
     gene            4263..5060
                     /locus_tag="VFR57_02030"
     CDS             4263..5060
                     /locus_tag="VFR57_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0258181.1"
                     /translation="MILELDGKRVFAYTAAHELDPAKPSVAFVHGAGLDHSWFGLQSR
                     YFGYHGRNVLALDFPGHGRSEGPPLPTIAAMADWVSSVLKKLKIRKASIVGHSMGSLV
                     SLEFAARHPGQCERVALIATGFPMKVAGPFLEAARNNDYAAFDMQTIWGHAPQIALGA
                     NPNPGMWMYGDSLARLERLAPGVLYNDLKACNEYGSGAESALKVNCPVLFILGRRDVM
                     TPPRAVKLLQEKIPGARTVEVAPSGHSLMAEAPDATLDALIEFISSG"
     gene            complement(5044..6366)
                     /locus_tag="VFR57_02035"
     CDS             complement(5044..6366)
                     /locus_tag="VFR57_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0258182.1"
                     /translation="MNAAPSVNARTERLLAAPIVPTLARLSAPGVLLVAFQSLVSVGD
                     TYFVGRLGTGPLAGLALVFPLLMLLQMTSAGAMGGGVSSAIARALGSGDAAAARRLVA
                     HALVIAAAMGAAFSILVLLPAELIYELLGGRGETLEHALRYSSIVFGGAIVVWIANTL
                     SSVLRGSGNMLTPALTLIGAALVHLPLSGILVSRIGIAGAGIAYVTTFGIAALVMGLI
                     VRRSSLLSPRREDWKLEWRLFREILRVGAISTLSAVQTVLTAVILTGFVGRHGAAALA
                     GYGVGLRLELLQIPLVFAVGQALVVLVGTHIGAGRAAGAKRIAWVGAGFAACISLVIG
                     VAVSTFPLAWVGIFSSDAAVLEHGSSYLRTVAPFYPLLAAGIALYFASQGAGRVLRPI
                     LAGTVRLAIVIAGGLMVESLQGVFVVIAAAMIAFGALTVWFVASTRWK"
     gene            6416..7159
                     /locus_tag="VFR57_02040"
     CDS             6416..7159
                     /locus_tag="VFR57_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0258183.1"
                     /translation="MIELLQDPQVWIAFFTLTALELVLGIDNIIFISILVDKLPPVQR
                     DRARRIGLGAAMLMRIGLLLVLAWIIGLTEPLFTVFNEGISGRDLILIGGGLFLLWKS
                     VMEIHQLLEGEEGERSAAVRASFSAVIGQIMVIDLIFSLDSIITAVGMVPWIEVMIAA
                     VIASVALMMVFAGPIGRFVSAHPTIKMLALSFLVLIGVLLIAAGFDHDVPKGYVYFAL
                     AFSVAVEMLNIRLRKRSKPVHLHEPYRRG"
     gene            complement(7144..7506)
                     /locus_tag="VFR57_02045"
     CDS             complement(7144..7506)
                     /locus_tag="VFR57_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU0258184.1"
                     /translation="MRNVLVAAGWGWATAIVWLSLTPSPRELDIAYGDKLGHIAAYGL
                     LMLWFTQLYVARRSRLAYAGVFVAMGVALEFLQGALGYRSYDIFDMYANTLGVALGWA
                     LGGMLRPRFPRPADQPRR"
     gene            complement(7506..8585)
                     /locus_tag="VFR57_02050"
     CDS             complement(7506..8585)
                     /locus_tag="VFR57_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU0258185.1"
                     /translation="MKDDPRYLRGARSFVQGRFETVAREAFDDGWTPEEADSRELKTT
                     VAIESARSIISHNDSPDVGFSQSINPYRGCEHGCVYCYARPSHAYLELSPGLDFETKL
                     FAKANAAELLRAELAKPGYRPSPIALGANTDCYQPIERKYRITRQILEVLAECEHPVT
                     MVTKSALIERDLDLLKELGWKNLVKVFVSIGTLDRELARKLEPRAASPQRRMDVVRRL
                     NENGVPCGVMIAALIPALNDTTMEQVLEAAAAAGAREAAYVVLRLPYELKAVFKEWLA
                     EHYPQRAEHVMSIVRQMRGGRDNDPCFGTRMSGTGNYAQLMEKRFEIACRRFGVNRRE
                     APQLDCTRFRPPASTSPAGAQLELL"
     gene            8602..9786
                     /locus_tag="VFR57_02055"
     CDS             8602..9786
                     /locus_tag="VFR57_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258186.1"
                     /translation="MNSTRRNVALLAACQALLFTNNSTLIAINGLAGLALAPHVGLAT
                     LPVTGWVLGGAIGTMPASLHMKRVGRQRGLTTGTLWGIVGALICGAAIWAQSFWLLCF
                     GTLVFGVYNAYVQYYRFAAADVASRDFKNTAISLVVAGGLVGGILGPTTSRYTIGLIA
                     PKFMGAYLVLIGFAVATMILLRFIRIPTPGPAERSAAGRPLWEIAAQPKYLIAVAAGA
                     IGYGVMNFLMTSTPIAMQVCGHPYGDAAFVISSHIIGMFAPSFITGRLIRSFGALQVM
                     FAGALLNFAAVGIALAGIAVANFWWSLVILGVGWNFLYIGGTTLLTESYRPEERAKAQ
                     GLNEQAIFTMMAISSLTSGLTVTTAGWERVNLLALPLVAAVAIAIAWFALRERARKAG
                     AP"
     gene            9783..10880
                     /locus_tag="VFR57_02060"
     CDS             9783..10880
                     /locus_tag="VFR57_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU0258187.1"
                     /translation="MSRPAPAAVGVPLEQVDTPALILDLPAFERNQRTLFEAVRGRGV
                     RVRPHAKTHKCPEVGKRQLAMGAVGMCCQKVSEAEAMVDGGIGDVLISNEIVGARKLE
                     RLAALSRRATIGVCVDNAENLQAIAATGAKLDVYIEIDVGMRRCGVAPGEPAVALARK
                     ILEHKHLRFAGLQAYQGRAQHLRGMEERHRAIEVAAQHVQHTKRLLAQAGIACPRVTG
                     AGSGTFMLEVEAGAWDEIQPGSYAFMDADYARNEWAAPLPRFEHALFVLATVMSRPSA
                     GVAIVDAGLKASSVDSGMPGVWQRKELKYTHASDEHGWIEGTPSPALGEKLLLVPGHC
                     DPTINLYDWYVVVRNEIVVDLWAITARGAVT"
     gene            complement(11133..11366)
                     /locus_tag="VFR57_02065"
     CDS             complement(11133..11366)
                     /locus_tag="VFR57_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258188.1"
                     /translation="MASDSIYCEILKRAAEIAGGKDRFAQHLDVTLEDMHEWMQGKAT
                     ARGGIYIIALDILLHSRSADDLPAESPKEKPAQ"
     gene            complement(11383..11511)
                     /locus_tag="VFR57_02070"
     CDS             complement(11383..11511)
                     /locus_tag="VFR57_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258189.1"
                     /translation="MLTPLTRNTLAAAVLAALAGIAATQDARREDRRQDPPQREVV"
     gene            11618..12430
                     /locus_tag="VFR57_02075"
     CDS             11618..12430
                     /locus_tag="VFR57_02075"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEU0258190.1"
                     /translation="MTPRPSLLSALRRLLRPLVKLLTAQGLTYPMLADLLKQIYVDVA
                     VREFALGEGVPTDSRVSLLTGVHRKDVKRLRGAPVTDEAMPAAVALGAQLAAAWTTRR
                     ELRDRNGRPRPLPRLASQGGGRSFEALVASISKDIRARPLLDEWLRVGAVELDDLDRV
                     VLRAAAFVPSRGFDEKAFYFGHNLHDHIAAAAHNMQGAAPPFLERSVHYEGLEAGSVA
                     ALAALAEKSGMQAVQTVYRKAKESEARDGKADGEKQRVTFGVYFYSEKLERS"
     gene            12427..13521
                     /locus_tag="VFR57_02080"
     CDS             12427..13521
                     /locus_tag="VFR57_02080"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEU0258191.1"
                     /translation="MKRLLAALACVSLAHACVDLGGIGGTGISDGAACVDPGGIGGTG
                     ISDGGIGGTGVRAETELGVFGVITGFASICVNGIELHYDAATPVSLNGAPAQAGALAV
                     GQVVMVQAVASSTWARAGAIHIVDAAVGVAAVRAEGLTVDEQRVRVEPATVMGAGLAR
                     DQLAGRTLRVSGLRAADGTIVATRIDRAPAHGMAVAQAPDLGAGRFSVQGYVADVGQQ
                     DLRIGATSFDVSREMAGQLERDRLVRVSGRIEGGRRIVERADVLSRPLDSHPERTLRV
                     EARSGHALGDPRGGSGSGSGRDGAERVDRSGPGGGDRPERTERSGRSERPERPDRVDR
                     SGSGGGDRPERTERSGRSERPDRVDRSGKH"
     gene            complement(13763..14041)
                     /locus_tag="VFR57_02085"
     CDS             complement(13763..14041)
                     /locus_tag="VFR57_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HEU0258192.1"
                     /translation="MPDLTLARRLELVREIVDKGLTEPTARKWLGRYPAEGEGGLHDR
                     SSFPRRIPRKIKPEKPLAIVELRRRRRRLTQARIAASLGPPKALSDAF"
     gene            14189..14992
                     /locus_tag="VFR57_02090"
     CDS             14189..14992
                     /locus_tag="VFR57_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258193.1"
                     /translation="MRFSKLWMAVFISVGASCACAQVSVGAGAEFTTGKYGGTESTQE
                     LYLPFTGKLQTGLWSFKLTVPYLVVSGPANVIGGGDDRIPIDSSGNGSGGSSGVSDAG
                     SATRTTSGLGDIVARASYSLLQETSSAVGLDVGARVKFGTADEAEGLGTGETDLSLQA
                     DLYKPLGAAMLFATLGYRWYGDPPGIELRDVPYFAVGASYRLGGDTAVGLAYDYRPHI
                     VDGGLSISEVSLYWSRRFAREWKLQLYAIVGLSDGSPDVGVGSFLEYQL"
     gene            complement(14989..15201)
                     /locus_tag="VFR57_02095"
     CDS             complement(14989..15201)
                     /locus_tag="VFR57_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258194.1"
                     /translation="MKVEISICQDMALSICSDVHKQNQFTVICGRHAYYLAALGTVAQ
                     CKLLLLSSSAMYNTGAVALPPSRSNP"
     gene            15263..16186
                     /locus_tag="VFR57_02100"
     CDS             15263..16186
                     /locus_tag="VFR57_02100"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HEU0258195.1"
                     /translation="MHVPALILACALAGTALAEGPVQSGKRGDLGIRYWLSSGETKTS
                     HNAQPLDPTLGNPTSVLLYESLDANVLELFGRRDFHGRWFLKGILGVGRVNNGSFDDE
                     DFNAGQVKFSDTTSSVNQGRIGYGVLDLGWNVWVLGEGRTTIGAFVGYSQWTEEVDAY
                     GATDHLGFIGGDIPPDVKVVTNKVAWKSLRIGLAADVRFTERSRLALDVALIPYSKVR
                     NEDSHHLRNDLGPVPNILVEGDGRGVQLDAELSYEIRRRTELGIGVRYWYVETTDGTR
                     SLPNRPDVPNLPIVELYSMRTGVTLTLRRLW"
     gene            complement(16676..17173)
                     /locus_tag="VFR57_02105"
     CDS             complement(16676..17173)
                     /locus_tag="VFR57_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258196.1"
                     /translation="MNNLKTIVVIATLGLSSACLAADPVVKSAIGGGLGGAAGAAVGQ
                     EVGGKKGAVVGGAVGGALGGAASKCGSGAVVGGAVGGAAGTAIGQSAGGKTGAVVGGG
                     TGGAAGAAIGCGVTSDKKPQQAAAKPVPVAATVVTAPVRVEVSGDCGRKHNKGKGWAK
                     GHTKC"
BASE COUNT         2972 a         5758 c         5743 g         2982 t
ORIGIN      
        1 ggagctcgag aagcttgccg atgtcgccta ccacccgaag gacatcgccg cgaccatctc
       61 gagcggctcg tacctcaccg agggcatggt gattgcgcca tgctcgatga agacgctcgc
      121 cgccgtggcg cacgcgcatg ccgacgagct cgtgtcgcgc gccgccgacg tggtgctgaa
      181 ggagcggcgc cggctcgtgc tgctgccgcg cgaaacgccg ctcaacctcg cgcacctgcg
      241 caacatggtc gcggtcacgg aaatgggcgg gatcgtgttt ccgccggtgc cggctttcta
      301 tgcggcaccc aaatcgctcg atgacctcgt ggcgcacacc tgcgcgcgcg tgctcgacct
      361 cttcggcgtg cactccccta ggctcgcgcg ctggcagggg atgaaaggaa ttcccgaaga
      421 gcgcggttga aagcctcggg ctgctcgatg ttcgcgatgt gcgaagcctg cgggatcacc
      481 acgagcttcg cgcccggaac gttctcggcg atgtagcgcg tgcccggcgc ggcggcgtcc
      541 tgctcgccgg taatggcgag caccggcagc ttgatgccct tgagcttcgc agtcgtgttg
      601 aggcccatga tcgcgcggcc gcagcctacg tagccggcga ccggcgttgc ggcgatctgc
      661 tcggcgatct tcctcgccgc ggcgctggag cggaacggcg gcgtgaacca gcgctcgagc
      721 gtcgaatcca caagcgcggc catgcccttt gtttcggcga tgcggatgcg ttcctcccat
      781 tgcgtgacgg cctcgggcgg ctgagtgtgg gtagtgtcgg cgagcaccat gcgctcgaac
      841 ggcccgcggc cgcggatagc gatcgtctgg ccgatcatgc cgccgatcga cagcccgcag
      901 tagactgcgc tcgtcacctt gagggaagcg gcaagaccga gaacatcgtc ggccagcacg
      961 tcgaatgtgt aggggcctgc cggggcgctc gtcagcccgt gaccccgcat gtcataggcg
     1021 aggatgcgat ggctcccctt gaacgcctcg atctgcggat cccacatgcg caggctgcag
     1081 gcgagcgaat ggctgagaac gagccatggc gcgccgtcct tgccgtgcag ctcgtagtga
     1141 atctcaatgc cgttgacctt gaccttcatg tcgcctccat gatgagcgga atgagcatct
     1201 cgtcctcgct ggtgccgccg tggttgccga tgtgcaggtg gcgtgactcg cccggcgtcc
     1261 agtccttcac cgcgtagcgg ccgcgcatga cgagcgcgac gtcgccgatg cgctcggcga
     1321 agcgcgggtg cggcgcaccc ggcccgaacc agccttcgtc caccagcgct cggctcggca
     1381 tgacctccgc gcgctcgccg agccagtccc ttgctttctg gatgaacagg ggagcgtcgt
     1441 ggacgtggca gtacgccacg cgccgttcgc cgcacaaggg aaacctcagg agagcagcga
     1501 gcgatgccgg cagctcgagc gattcctcgg gtgcaacgtc gatgaagccg tggtcggcgg
     1561 tggcgatgat gagcgactcg gtgccggcga gccgcgcgac catctccccg aacgcggcat
     1621 cgatcttggt gaattgctcg aaggcttcgg cgctttccgc gccgtagcgg tgcgacacca
     1681 tgtcgtagac cggccagtat gcataaatga atttgcgggt gtcggcggac ttcaccgcgg
     1741 cctcgacgcc cgcgacgagc tcgtcgaggc tctcgtatgc gatccgctgc gcgccgcggc
     1801 agtggcggat gttgtactcg gagtcgatga tgtccttgta cgtaataacg aaggaggcgt
     1861 ccgtcaccga ttcgaaaatc gaatcgcaga catagatctg ctccggcgtc actccgcggc
     1921 gcgcgagcgg caggtagtcg ccgcggctcc tgaaaggaag cgccgcgctc acgcagcccg
     1981 cttcgccgaa ataggtgaac cagccggtga gcccgtgctc gagcggcgtg cgcccggtgt
     2041 aagaggtggt gatcgccgac gcggttgtcg agggaaacac cgaggtcagc gagccgcggc
     2101 gacggcccgc gagcgcgctg cccgcgccgc ggcgcaggag gtagttgtcg cccaggccgt
     2161 ccacgatgag gagcacgatg tttcgcgcgt cgcccagttc gctcgcagga aggtttgcaa
     2221 gcgcaggatg cagcggcgcg ccgccgcgcg ccgcgacgat cgaggcgatc agattgacga
     2281 gactgccgcc ggaataatcg ggtcgcatga cctcgggtcg tacggcccga ttttaggcgt
     2341 acaattaatg catgcggcac gaagttcacc tgcacgccga catcccgatc ctcgaaggcg
     2401 tttcacggcg ccagctcgag caggcgttcg cgccgctgct cgagtatctc gacgccgacg
     2461 gcctcgccga cgtgaaaagc ctcgagcccg acgagccggg catcaagtac gacgagaagg
     2521 agctgatcct caccgtgtgc tggaccggtg agatcgggcg cagcttccac ggcgcgctcg
     2581 aagcgacgct cgagagcctc ggaccgtact gctacgaggc cgtcgaggtc gatctcacgc
     2641 tctaccacga gaacggcgag caggaatcga agctgctctt cgtcggcccg acggcgcacg
     2701 cgatccacga ggcgcagcgc cggtgcatgg tcgaagatgt cgcggcgatc ctcgcgcgcc
     2761 agttcggcaa ggaacaagtg gcgcaggtga cggcgctcgt gaacgagctc ttcgaccgcg
     2821 actggaaaga aaaggcttcg cagcagaaga agcccgagcg cgccttcgaa aactacactc
     2881 gcccttcgcg caaaaacctg cattaagaac cacaaggaga ttcctctgac gaggaatctc
     2941 aagactttca gaaaaggcca tggcagggcc taaatatccg tccctcgata cgctcgcgtt
     3001 gcatgcgggc cagtcgcccg accctgcgac cggcagccgc gcagtcccga tctaccagac
     3061 cacgtcctac gtgttccgcg acacggagca cgcggcgtcg ctcttcaaca tggagcgcgc
     3121 cgggcacgtc tattcgcgca tctccaaccc gaccgtagca gtactcgaag agcgcgtggc
     3181 cgcgctcgag ggcggggtcg gcgcgatcgc caccgcttcg ggccaggcgg cgctgcacct
     3241 cgcgatcgcg accctcatgg gtgccggcgg gcacatcgtc gcctccaccg cgctctacgg
     3301 cggctcgcac aacctcctcg cctacaccct caagcgcttc ggcatcgaga ccacatttgt
     3361 cagcccgaga tcgctttcac agtggaaaga ggcgattggg ccgcagactc ggcttctgtt
     3421 cggtgagacg ctcgggaacc cgggcctgga cgtcctcgac atcccgtcgc tcgcgcagat
     3481 cgcgcacgcg gccaaggtgc cgctcctggt cgacgcgacc ttcaccaccc cggtgctgat
     3541 gaagcccttc gcgctcggcg cggatctgct ttaccactcg gcgaccaagt tcctcggcgg
     3601 gcacggcgtc gcgatcgccg gcgtgctggt cgattccggt cgcttcgact gggaggcctc
     3661 gggcaaattc gagacgctga ccgcgcctta cgccggattt cacggaatgg atttcgagga
     3721 ggagtcgggc acgcgggcgt tccttttgcg cgcacgccgc gagggcctgc gcgacttcgg
     3781 cgcttgcatg gcaccgatga ccgctttcca aatcctccag ggcatcgaga cactgcacct
     3841 caggatgccg cggcatgtcg aaaatacccg gaaggtcgtc gcatttcttt cgaagaatcc
     3901 tgctgtggaa aaggtgatct atcccgagct tcccgaccat ccggaccatg agctcgcgag
     3961 gaggctccta ccccatggct gcggcgcggt gttcaccttc gagatcaagg gcggcaggcc
     4021 cgcgggcaag cgctttatcg agtcgctgcg gcttttttcg cacctcgcca acgtgggcga
     4081 cgcgaagtct ctcgtcatcc atcccgccag cacaactcac taccgcatga gcgacgagga
     4141 tttgaggaag gcgggcatca ccgccggcac ggtacgactc tcgatcggac tcgaggatcc
     4201 gcaggacctg atcgaggacc tcgagcgcgc gctgcacgcc tcggcgaagt gaacttcgcg
     4261 cggtgatctt ggagctcgac ggcaagcggg tcttcgccta cacggcggcg cacgagctcg
     4321 acccggcgaa gcccagcgtc gcgttcgttc acggcgcggg gctggatcac tcctggttcg
     4381 ggctgcaaag ccgctacttc ggctaccacg ggcgtaacgt ccttgcgctc gatttcccgg
     4441 gacatggccg cagtgaaggc ccgccacttc cgaccatcgc ggcaatggcc gactgggtgt
     4501 cctcggtgct taagaaacta aagattcgaa aggcgagcat cgtcggccac tcgatgggtt
     4561 cgctcgtctc gctggagttc gccgcgcgtc atccggggca gtgcgagcgc gtcgcgctga
     4621 tcgccacggg ctttccgatg aaggtggccg ggccatttct ggaagccgcg cgcaacaacg
     4681 actacgccgc cttcgacatg cagacgatct ggggccatgc gccgcagatc gcgctcggcg
     4741 ccaatcccaa ccctggcatg tggatgtacg gcgactcgct tgcgcgccta gagcgcctcg
     4801 cgccgggcgt gctttacaac gacctcaagg cgtgcaacga atacggctcc ggcgccgaaa
     4861 gcgcactgaa agtgaactgc ccggtgctct tcatcctggg caggcgcgac gtgatgacgc
     4921 cgccgagggc ggtgaagctc ctccaggaaa agattcccgg cgcaaggacg gtcgaggtcg
     4981 ctccctccgg gcactcgctg atggccgaag cgcccgatgc gacgctcgat gcgctgatcg
     5041 agttcatttc cagcgggtag aagccacgaa ccagacggtg agcgcgccga acgcgatcat
     5101 cgcggcggcg atcacgacga aaacgccctg cagcgactcg accatcagcc cgcccgcgat
     5161 cacgatcgcg agccgcaccg tgccggcgag tattggccgc agcacgcgcc ccgcgccctg
     5221 cgaggcgaag taaagcgcga tccccgcggc gagcagcggg tagaacggcg ccacggtgcg
     5281 caggtagctc gagccgtgct cgagcaccgc ggcgtcgctg ctgaagatcc ccacccaggc
     5341 gagcgggaag gtcgatacgg cgacgccgat cacgaggctg atgcaggcgg cgaagcccgc
     5401 tccgacccag gcaatgcgct tcgcgcccgc ggcgcggccg gcgccgatgt gtgtgccgac
     5461 gagcaccacg agcgcctggc cgaccgcgaa taccagcggg atctgcagga gctccagtcg
     5521 aagcccgacc ccgtagccgg caagcgccgc agcgccatgg cgcccgacga agccggtaag
     5581 gataacggcc gtcagcacgg tttgaaccgc cgaaagcgtc gagatcgcgc ccacgcgcag
     5641 gatctcgcgg aacagccgcc attcgagctt ccagtcctcg cggcggggcg agagcaggct
     5701 cgagcgccgc acgatcagtc ccatgacgag agccgcgatc ccgaaagtcg tgacgtaggc
     5761 gattcccgcg cccgcgatgc cgatgcgtga gacgagaatt ccggacagcg gcaggtgcac
     5821 gagcgccgcg ccgatcagcg tgagcgcggg ggtgagcatg ttgccgctcc cgcgcagcac
     5881 gctcgagagc gtgttggcga tccagaccac gatcgcgccg ccgaagacga tgctcgagta
     5941 gcgcagcgcg tgctcgaggg tttcgccccg gccgccgagc aactcgtaga tcaattctgc
     6001 aggtaacaac accaggatcg agaaggcggc gcccatcgct gcggcgatga cgagcgcatg
     6061 cgcgaccagt cgccgggcgg cggccgcgtc cccggagccc agagcgcgcg cgatcgccga
     6121 agataccccg ccgcccatcg cgccagcgga agtcatctgc agcagcatga ggagcgggaa
     6181 gacgagcgcg aggccggcga gcggcccggt gccgaggcgg ccgacgaagt acgtatcgcc
     6241 gaccgaaacc agcgactgga atgcgaccag caacacgccc ggcgccgaca ggcgcgcgag
     6301 cgtcggcaca atcggcgcgg cgagcagccg ttcggtgcgc gcattcacgg agggcgccgc
     6361 gttcatgggg cgcgccattg tatgatcgcg cgcttgcccg caggaggatg acgccgtgat
     6421 cgagctcctt caggaccctc aagtctggat cgccttcttc acgctcaccg cgctcgagct
     6481 cgtgctcggc atcgacaaca tcatcttcat ctcgatcctg gtcgacaagc tgccgccggt
     6541 gcagcgtgac cgcgcgcggc gcatcggcct cggcgccgcg atgctgatgc gcattggcct
     6601 gctgctcgtc cttgcctgga tcatcggcct taccgagcct ctgttcaccg tgttcaacga
     6661 aggaatctcg gggcgcgacc tgatcctgat cggcggcggg cttttccttt tgtggaagag
     6721 cgtgatggag attcaccagc tgctcgaagg cgaggagggc gagcgctcgg ctgccgtgcg
     6781 tgccagcttc tctgccgtga tcggccagat catggtgatc gacctcatct tttcgctcga
     6841 ctcgatcatc accgcggtcg ggatggtgcc gtggatcgag gtgatgatcg ctgcggtaat
     6901 cgcgtcggtg gcgctaatga tggtattcgc cgggccgatt ggccgcttcg tctcggcgca
     6961 cccgacgatc aagatgctcg cgctctcttt cctggtcctg atcggcgtgc tgctgatcgc
     7021 ggccggcttc gaccacgacg tccccaaggg ctacgtatac tttgcgctcg ctttctccgt
     7081 cgcggtggag atgctcaaca tccgcctgcg caagcgctcc aagcccgtgc acctgcacga
     7141 gccctaccgc cgcggctgat cggccgggcg cgggaagcgc gggcgaagca tcccgccgag
     7201 cgcccagccg agtgcgacgc cgagcgtgtt tgcgtacatg tcgaagatgt cgtagctgcg
     7261 gtagccgagg gcgccttgca ggaattcgag cgccacgccc atggcgacga acacacccgc
     7321 gtacgccagg cggctgcgcc gcgcgacata cagctgcgtg aaccagagca tcagcagccc
     7381 gtaggcggcg atgtgcccta gcttgtcgcc gtaggcgatg tcgagctcgc gtggcgacgg
     7441 cgtgagcgac agccacacga tcgccgtggc ccatccccag ccggcagcga ccagcacgtt
     7501 cctcatcaca gcaactccag ctgagctccg gccggtgacg ttgaagctgg cgggcgaaac
     7561 cgcgtgcagt cgagctgcgg cgcctctctt cgattcacgc cgaagcgccg gcacgcgatc
     7621 tcgaagcgct tctccatcag ctgggcgtag ttccccgtgc ccgacatccg cgtgccgaag
     7681 catggatcgt tgtcgcggcc gccgcgcatc tggcgcacga tgctcatcac gtgctccgcg
     7741 cgctgcgggt agtgctccgc gagccattcc ttgaataccg ctttcagctc atacggcagg
     7801 cgcaggacca cgtatgccgc ttcgcgtgcg cccgccgcgg cggccgcctc gagcacttgc
     7861 tccatcgtcg tgtcgttgag cgccgggatc agcgccgcga tcattacgcc gcagggaacc
     7921 ccgttctcgt tcagtcgtct caccacgtcc atgcgccgct gcgggctcgc ggcacgcggc
     7981 tcgagcttgc gcgcgagctc tcggtccagc gtgccgatcg acacaaatac ctttaccagg
     8041 ttcttccaac caagttcctt caatagatcg aggtctcgtt cgatcagagc cgatttggtc
     8101 accatcgtga ccgggtgctc gcactcggcc agcacttcca ggatctgccg ggtgatgcgg
     8161 tacttgcgct cgatcggctg gtagcaatcg gtgtttgcac caagcgcgat gggactcggc
     8221 ctgtagccgg gcttcgcgag ttcggcgcgc agcagttcgg cggcgttggc cttggcaaag
     8281 agcttggtct cgaagtccag gccgggcgag agctcgagat aggcgtggct cggcctcgcg
     8341 tagcagtaga cacagccatg ctcgcatcct ctataaggat tgatggactg cgagaagccg
     8401 acgtcgggcg agtcgttgtg cgagatgatc gagcgcgcgc tctcgatggc gacggtggtc
     8461 ttcagctccc ggctgtctgc ctcctcaggc gtccagccgt cgtcgaacgc ttcgcgcgcc
     8521 accgtttcga aacggccctg cacgaaagag cgcgctccac gaagatagcg ggggtcgtcc
     8581 ttcatgcctg atactgtata tgtgaacagt actcgacgca atgtcgcgct gctggcggcg
     8641 tgccaggcct tgctgttcac caataactcg accctgatcg ccatcaacgg cctggcgggc
     8701 ctggcgcttg cgccgcacgt gggcctcgcg acgctgcctg tcaccggctg ggtgctcggc
     8761 ggcgcgatcg gcaccatgcc ggcatcgctg cacatgaagc gcgtcggtcg tcagcgaggg
     8821 cttaccactg gaacgctctg gggaatcgtt ggagccctta tctgcggcgc ggcgatctgg
     8881 gcgcagagct tctggttgct ttgcttcggc acgctcgtgt tcggtgtcta caacgcctac
     8941 gtccagtatt accgcttcgc cgcggccgac gtcgccagcc gcgacttcaa gaacaccgcg
     9001 atctcgctcg tggtcgccgg cggcctggtc ggcgggatcc tcggaccgac cacgagccgc
     9061 tatacgatcg ggctcatcgc gccgaagttc atgggtgcgt acctggtgct gatcggcttc
     9121 gcggtggcga cgatgatcct gctgcgcttc atccgcattc cgacgccagg gccggccgaa
     9181 cgctcggcag ccggacggcc gttgtgggag atcgcggcgc agccgaaata cctcatagcg
     9241 gtagcggcgg gtgcgatcgg ctacggggtg atgaacttcc tgatgacctc gactccgatc
     9301 gccatgcagg tctgcggcca cccttacggc gacgccgctt tcgtgatctc ctcacacatc
     9361 atcggcatgt tcgcgccgtc gttcatcacc gggcgattga tccggagctt cggcgcgctg
     9421 caggtgatgt tcgcgggcgc gctgctcaac ttcgccgccg ttggtatcgc cctcgccggc
     9481 attgcggtag cgaacttctg gtggtcgctc gtgatccttg gagtaggctg gaacttcctc
     9541 tacattggcg gcacgacgct tcttaccgaa tcctaccgcc cggaggagcg ggcgaaggcg
     9601 caaggcctga acgagcaggc tattttcacc atgatggcga tctcttcgct tacctccggg
     9661 ctcaccgtga ctaccgccgg ctgggagcgc gtcaacctgt tggcgctgcc gctcgtcgcc
     9721 gcggtcgcca tagcgatcgc gtggttcgcg ctgcgcgagc gcgcccgcaa ggcgggtgcg
     9781 ccatgagtcg gcccgcgccc gccgcggtcg gcgtgccgct cgagcaggtc gacacccctg
     9841 cgctgattct tgatctgcct gcctttgaaa gaaatcagcg cacgctgttc gaagcagtga
     9901 gagggcgcgg cgttcgggtg cggccgcacg ccaagacgca caaatgcccc gaggtcggga
     9961 agcggcagct cgcgatgggc gcggtcggca tgtgctgtca aaaggtgtcc gaggccgagg
    10021 cgatggtcga cggcgggatc ggcgacgtgc tgatctcgaa cgagatcgtg ggcgcgcgca
    10081 agctcgagcg cctcgccgcg ctttcgcgac gagcgacgat cggagtctgc gtcgacaacg
    10141 ccgagaacct tcaagccatc gcggcgaccg gcgccaagct ggacgtttac atcgaaatcg
    10201 atgtcggcat gcgccggtgc ggtgtggctc cgggcgaacc ggccgttgcg ctcgcgagaa
    10261 agatcctcga gcacaagcat ttgcgcttcg cagggcttca ggcctaccag ggccgtgcgc
    10321 agcacctccg cggcatggag gagcgccatc gcgcgatcga ggtcgcagcg cagcacgtcc
    10381 agcatacgaa acgactgctg gcgcaggcag gcatcgcctg tccgcgcgtc accggcgcag
    10441 gcagcggcac tttcatgctc gaggtcgagg ccggcgcgtg ggacgagatc cagcccggct
    10501 cgtacgcctt catggacgcc gactacgccc gcaacgaatg ggccgcgccg ctgccgcgct
    10561 tcgagcacgc gcttttcgtg ctcgccaccg tcatgagccg gccttcggcc ggcgttgcca
    10621 tcgtggacgc cggcctcaag gcctcgagcg tcgactccgg tatgcctggg gtgtggcaac
    10681 ggaaagagct caaatacacc cacgcgtccg acgagcacgg atggatcgaa ggcaccccta
    10741 gcccggcgct aggcgagaag ctgctcctcg tgcccgggca ctgcgacccg acgatcaacc
    10801 tctacgactg gtacgtagtc gtgcgcaatg agatcgtggt cgatctctgg gcaattaccg
    10861 ctagaggcgc agtgacttaa cgttcgttag cgttcgccac cggacgctgg aatccggcat
    10921 gtagcgggga ccctatactt tgttttgcgg caagtgaatg gcgtgccgcg agcccgccgc
    10981 attggccctt ctcgcaatcg ggttggcagg cttaaggctt caaccgccgc aagcacacgt
    11041 gagtttcctt ctccgagcgt ccacccgccc ggagatgaag cccacagaac tggaccccct
    11101 ggagggcttt ttcctatttt ggctcttagc ctctactgcg ctggcttttc cttcgggctt
    11161 tctgcgggta agtcgtcagc gctgcggctg tgcaagagga tgtctaacgc aattatgtag
    11221 ataccgcccc gcgctgttgc tttgccttgc atccactcgt gcatgtcttc taatgtcacg
    11281 tcgaggtgct gtgcaaacct atctttgccg cctgcgattt cagccgcacg cttcaaaatt
    11341 tcgcaataga tactgtcgct cgccatgcga aggacttcct ccctacacga cttcgcgctg
    11401 cggcgggtct tggcggcggt cctcgcggcg cgcgtcctgc gtcgctgcga tgccggcgag
    11461 cgccgcaagc acggcggcgg cgagcgtatt gcgggtgagc ggggtcagca tggtggacgt
    11521 cggttggcgg gttgcacggt ccaagcctac gtgggatgat ttcccacgtc aatctcggcc
    11581 tgtgtgaaat attcccacgg ctgctatagt ccgagcgatg accccccgtc cctcgctcct
    11641 cagcgcgctg cgccgcctgc tgcggccgct ggtgaagctg ctcaccgccc agggcttgac
    11701 ctacccgatg ctcgccgatc tgctcaagca gatctacgtg gacgtggcgg tgcgcgagtt
    11761 cgcgctgggc gagggcgtgc cgaccgacag ccgggtcagt ctgctgaccg gtgtacaccg
    11821 caaggacgtt aagcgcctgc gcggcgcgcc tgtcaccgac gaggcgatgc cggcggcggt
    11881 cgcgctcggc gcccagctcg ccgccgcttg gaccacccgg cgcgagctgc gcgaccgcaa
    11941 cggccggccg cggccgctgc cgcggctcgc gagccagggc ggcgggcgct cgttcgaggc
    12001 gctggtcgcg agcatcagca aggacatccg cgcacgcccg ctgctcgacg aatggctgcg
    12061 ggtcggcgcg gtggagctcg acgacctgga ccgcgtagtg ttgcgcgccg cggctttcgt
    12121 gccgagccgc ggcttcgacg agaaggcgtt ctacttcggc cacaacctgc acgaccacat
    12181 cgccgccgcc gcgcacaaca tgcagggggc ggcgccgccc ttcctcgagc gcagcgtgca
    12241 ctatgagggg ctcgaggcgg gctcggtagc tgcgctcgcc gccctcgccg agaagagcgg
    12301 aatgcaggcg gtgcagacag tgtaccgcaa ggcaaaggaa agcgaggcgc gcgacggcaa
    12361 ggccgatggc gagaagcagc gcgtcacgtt cggggtgtac ttctacagcg agaagctcga
    12421 gcgctcatga aacggttgct cgccgcgctg gcgtgcgttt cgcttgcgca cgcgtgtgtc
    12481 gacctgggcg gcatcggcgg caccggtatc agcgacggcg ccgcgtgtgt cgacccgggc
    12541 ggcatcggcg gcaccggcat cagcgacggc ggcatcggcg gcactggcgt gcgcgccgaa
    12601 accgagctcg gggtgttcgg cgtgatcacc ggcttcgcca gcatctgcgt caacggcatc
    12661 gagctgcact acgacgccgc gactccggtc tcgctgaacg gcgcgcccgc gcaggccggt
    12721 gctcttgccg tagggcaggt cgtgatggtt caggctgtgg cgagcagcac gtgggcgcgc
    12781 gcaggcgcga tccacatcgt cgacgccgcg gtgggcgtcg ctgccgtgcg cgcggagggc
    12841 ttgacggtcg acgagcagcg cgttcgggtc gagcccgcga ctgttatggg cgccgggctc
    12901 gcgcgcgacc agctcgccgg gcgaacgctg cgtgtgagcg gtttgcgcgc cgccgacggc
    12961 acgatcgtcg ccacgcgcat cgatcgcgcg ccggcgcacg gcatggccgt cgcgcaggcg
    13021 ccggacctcg gcgccgggcg cttctcggtg cagggctacg tggccgatgt ggggcagcag
    13081 gacctgcgca tcggcgccac gagctttgac gtgtcgcgcg aaatggcggg acagctcgag
    13141 cgcgatcggc tggtgcgcgt cagcggccgc atcgagggcg gccgccgcat cgtcgagcgc
    13201 gccgacgtgc tgtcaaggcc gctcgattca catcccgagc gcacgctgcg cgtcgaggcg
    13261 cggtccggcc acgcgcttgg cgatccgcgc ggcggcagcg gcagcggctc gggacgcgac
    13321 ggcgccgagc gcgtcgaccg cagcggcccg ggcggtggcg atcgccccga gcgcaccgaa
    13381 cggtccgggc gcagcgagcg ccccgagcgc ccggaccgcg tcgaccgcag cggctcgggc
    13441 ggcggcgatc gccccgagcg caccgaacgg tccgggcgca gcgagcgccc ggaccgcgtc
    13501 gatcgcagcg gcaagcatta gttgtgacgc gtcaagaggc tgttgcggga ctgagctaag
    13561 cgcgtagcag gcgcgaagcc cttgatgccc tgatataggc gatgccagtt gtagtacgaa
    13621 accaaggcga gcgcggcaca gttcctgcga tgcgcacgag cttcttggtg tccatgtgga
    13681 tcagatcgcc gggacgctcg tgctcgtaac gcacgatcgg ctcgcagggc tccaggtcgc
    13741 gcagccgaga tagcccggcg cttcagaacg cgtccgacag tgcttttgga ggtcccaggc
    13801 tcgccgcgat ccgcgcctga gttaggcgcc ggcgccggcg ccgcagctcg acgattgcca
    13861 agggcttctc cggcttgatt ttccttggga tgcgcctcgg aaacgacgag cggtcgtgca
    13921 gcccgccttc accttcggcg gggtaacgac ccaaccattt gcgagcggtt ggctccgtta
    13981 aacccttatc aacgatttct cgaaccaatt cgagtcgacg ggcgagcgtc aggtcaggca
    14041 ttcttgtgaa tgttcgttcg gctgagctcc ctgagagatt ggtgtggttc gcaccttcca
    14101 gtctctaaga ctcagtccga atgaacaaaa gcaacctatt gggccatcac aattagcgat
    14161 cgaattaccc ggaggaggtt cgcggcaaat gcgattcagc aagctgtgga tggccgtttt
    14221 catttccgtc ggcgcatcct gcgcgtgcgc gcaggtaagt gtcggcgccg gcgccgaatt
    14281 cacgaccggc aagtacggcg gcaccgagag cacgcaggag ttgtacctcc cgttcaccgg
    14341 aaagctgcaa actggcctgt ggagcttcaa gctgacggtt ccgtacctgg tggtttccgg
    14401 cccggcgaat gtcatcggcg gcggcgacga ccgtatccca atcgacagca gcgggaatgg
    14461 gagcggcggc agcagcgggg tcagcgacgc cggcagcgca acgcgcacca cttccggcct
    14521 gggcgacatc gtggcgaggg cctcctattc gctcttgcag gagacttcga gcgctgtcgg
    14581 gctcgacgtc ggtgcacggg tcaagttcgg caccgccgac gaagccgagg gcctcgggac
    14641 cggggaaacc gacttgtcgc tgcaggccga tctctacaag ccgctcggcg cggcgatgct
    14701 cttcgccacg ctcggctacc gctggtacgg cgatccgccg ggaatcgagc tgcgcgatgt
    14761 gccctacttt gcggtcggtg ccagctatcg cctaggcgga gacaccgcgg tcgggctggc
    14821 ctacgattac cgcccgcaca tcgtcgacgg gggactttcg atcagcgagg tgtcgctgta
    14881 ttggtcgcgg cgctttgcgc gcgaatggaa gctgcagctc tacgccatcg tcggtttgtc
    14941 ggacggcagc cccgacgtcg gggtggggtc gttcctggag taccagctct agggattact
    15001 tcttgaggga ggtaatgcaa cggcgccggt gttgtacatg gcgctggagc tgagcaacag
    15061 gagcttgcat tgagcgacgg tgccaagcgc cgccaggtag taggcgtgcc tgccgcagat
    15121 gacggtgaac tgattttgtt tatgcacatc gctgcatatt gacagcgcca tgtcttggca
    15181 gatggagatt tcgaccttca aggacgtcgg caagggcgac aacaatcgcg cgcaaatacg
    15241 agaacaccct gcgcgagctc gtatgcacgt tccagccctg attcttgcct gcgcgctcgc
    15301 cggcacggcg ctcgcggagg ggcccgtcca gtcggggaag cgcggcgatc tcggcatacg
    15361 ctactggctt tccagcggcg agaccaaaac cagccacaac gcacagccac tcgatccgac
    15421 gctcgggaat ccgacctcgg tgctcctcta cgagagcctc gacgccaacg tgcttgagct
    15481 cttcggccgg cgggatttcc acgggcgctg gtttctgaag ggcatcctcg gcgtcggccg
    15541 cgtgaacaac ggctcgttcg atgacgagga cttcaacgca gggcaggtca aattctcgga
    15601 taccacttcg tcggtcaacc agggtcgcat cggctacggc gtgctcgacc tgggctggaa
    15661 cgtatgggtg ctcggcgagg gccgcaccac gatcggcgcg ttcgtcgggt acagccagtg
    15721 gacggaagag gtcgacgcct acggcgcgac cgaccatctc ggcttcatcg gcggcgacat
    15781 cccccccgac gtgaaggtcg tgacgaacaa ggtcgcatgg aaatcgctgc gcatcggcct
    15841 cgccgcggac gtgaggttca ccgagcgcag ccgcctcgcg ctcgacgtcg cgctgattcc
    15901 ctacagcaag gtgcgaaacg aagacagcca ccacctgcgc aacgacctcg gcccggtgcc
    15961 caacatccta gtcgagggcg acggaagagg ggtgcagctt gatgccgagc tgagctacga
    16021 gatccggcgg cgtacggagc ttggtatcgg cgtgcgctac tggtatgtcg agacgaccga
    16081 cggaacacgc agcctgccca accgccccga tgtgcccaac ctcccgatcg tcgagcttta
    16141 ctccatgcgg accggcgtga cgctcacgct gcgacgactc tggtgattga atgctccggc
    16201 cgaaccgccg cgcctaacaa aatttcttgc taacgccttt gattaactat cggccgtatt
    16261 tcgttgttgg aactaggccg cgccggattt cgctgcaaaa cgctgcgaca agcgtcgtct
    16321 tgaaagcccg tcaaagatca gtaacactct cattattgtg agcaagggac ggactcgtca
    16381 tgaaatcgat cttggacgca tcgtttaagt acacgcccag ctcccacacg gacgtgcggg
    16441 aaaccttttg cagaattcgg cgagagcagg aggctcagat tcgggcacag gagcaggcgg
    16501 agattccgca aggccaacag gcaggcgcga attcgttggg cgggcactcc ctgggctagt
    16561 ctgcaccttt gatccggttt gagtcgcgct agcctctcgc gcgcaaccaa aaggcacgtg
    16621 cgtctatcgg ctgctccaac tggccaagcg caccagtggt gcctggtctg ctagttcagc
    16681 acttcgtgtg gccctttgcc cagcccttgc ccttgttgtg cttccgacca caatcgccac
    16741 ttacttcgac gcgtacgggc gcagtaacga ctgtcgccgc gacgggtacg ggcttggcgg
    16801 ccgcctgctg cggcttcttg tcggaggtca cgccacagcc gattgccgcc ccggctgcgc
    16861 cgccagtccc gccgcccacc accgcgcccg tcttgccgcc cgccgactga ccgatcgcgg
    16921 tgcctgcagc accgcccaca gcgccgccga ccacggcgcc agacccgcac ttcgacgccg
    16981 cgccgccgag cgcgccgccc acagcgccgc cgaccaccgc gcccttcttc ccgccgactt
    17041 cttggccgac ggctgcaccg gcggccccgc ctagcccgcc gccgatggcg ctcttcacga
    17101 cgggatccgc tgcaaggcac gcgctcgaga gcccgagcgt ggcgattact acgatcgttt
    17161 tgagattgtt catgaggccc tcccaaggct cagcagaata ccgcgcagcg gcgggtggcc
    17221 gccccgatag atgtcacaga acgctgtgga tggcacggac agcccctgcg gcccccgctc
    17281 gaaaccacgg atgcagcagg cagagaaagc gtccgcagcg tgaaaaaatg cgccgacaca
    17341 atccacacta cggttcaaga taaacaaaag ttcacatatt ttcaggatgc aaagataaga
    17401 aagcttcggc agtaagccca gaccgcaagt tttcgctgct ggccgaagta ctcag
//
LOCUS       DASTPD010000052         5749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_636907, whole genome shotgun sequence.
ACCESSION   DASTPD010000052 DASTPD010000000
VERSION     DASTPD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636907"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1051
                     /locus_tag="VFR57_02110"
     CDS             104..1051
                     /locus_tag="VFR57_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258197.1"
                     /translation="MKKVFALGIVLFAASALAQTYPARPVKLVVPFPAGSATDQIARV
                     MSQQFQEALGQPFVVENKPGAQGSIAATEVARAAPDGYTIMVTTNTPQAANVSLFKKL
                     NYDPVKDFAPIARLGTISFMIMVRPDFPAKNLKEFLAHAKANPGKLSAGYGSAGSQVS
                     QAMLRSMGGIDFVDVPYKGLPQAITDVLGGSIHFTFADLANALAQIKGGKLRGLAVTS
                     RKRSPLAPDVPAIAEELPGYELIAWFALVAPADTPAPVVTRLHDVTVKSLAKPEVKAR
                     FDSLGTDVAPMNPQELGGFIRSEIEKWAKMAKAAGIQPE"
     gene            1063..2265
                     /locus_tag="VFR57_02115"
     CDS             1063..2265
                     /locus_tag="VFR57_02115"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0258198.1"
                     /translation="MAGPLAGVRVLDLTTVVMGPYATQILGDFGADVIKVEPPEGDVM
                     RYAWPFRNPGMGSIYLNVNRNKRSVVLDLKKEAAREACLLLARRADVLVYNIRPQAMA
                     RLKLSYDEVRKVNPRIIYAGCFGYSQRGPYAAKAAYDDLIQGAAGLPWLLQKQGASEP
                     RYAPMIVADRSVGQQVASAINAALYHREKTGKGQRIDVPMFEHLLQIVLGDHLGGYTF
                     DPQYGEPGYARILTPDRRPYRTADGYVCALIYTNKQWKAFLDIIGKPEMLELPEYATQ
                     EARSQNYRQSYAMIAEEMKRRTTTFWLHALERGDIPVQRMNTLDDIVADPHLRAIDYL
                     HMVDHPSEGRIRALAVPSEWSESKPEYRRHAPRLGEHTREVLREAGLSDHEIHSLIDS
                     GAAKQAPL"
     gene            2325..3464
                     /locus_tag="VFR57_02120"
     CDS             2325..3464
                     /locus_tag="VFR57_02120"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU0258199.1"
                     /translation="MNAPHHDPSSVGTAQAQNARFSEPLRLRGGAELADFEIAYETYG
                     RLNEARSNAVLVCHALNASHHVAGFYENDPQNVGWWDNLVGPGKPLDTSRFFVVGSNY
                     IGSCFGSTGPASINPATGKPWGADFPVVTVEDWVEAQARLADRLGIERFAAVIGGSLG
                     AMQALQWTLSYPGRIRHSIVIAAAAKLTPQNIAFNEVARQAIMTDPDFHGGHYYEKGV
                     VPTRGLRIARMIGHITYLSGEAMSEKFGRLLRRGAPGFDFDIDFEIESYLRYQGDKFS
                     SYFDANTYLRITKALDYFDPAADYGGDLTKALARAKAAFLVVSFKSDWRFPPARSRDI
                     VRALLDNRRIVSYLELDAPGGHDAFLLEDSRYHTALRHYFSNIEL"
     gene            3461..4039
                     /gene="metW"
                     /locus_tag="VFR57_02125"
     CDS             3461..4039
                     /gene="metW"
                     /locus_tag="VFR57_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEU0258200.1"
                     /translation="MRSDLQAIVQWVPQGARVLDLGCGDGSLLRSLWQERQAPGYGVE
                     IEDQAVLACVENDVNVLQVDLESGLSLFSDGSFDCVILSETLQTIHRTELLLREMLRV
                     GREGIVSFPNFGHWSARLQIALGRMPVSETLPYQWYETPNVHHCTITDFEDLCRRIGI
                     RIRERLVLHDGKPVTMLPNLLGSVAVYRCTQS"
     gene            complement(4068..5369)
                     /locus_tag="VFR57_02130"
     CDS             complement(4068..5369)
                     /locus_tag="VFR57_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258201.1"
                     /translation="MSYSRLPAGLRLYLEPAPLAALLLGVSSGFTFAMIGATLSTRLA
                     QHGITRSAVTAFALTFLAYNFKFLWAPIVDNVRLPLIGRFGQRRSWLWLVGVLVMAAV
                     AVLGVADPQEALYMVAIAAILVGVAGATFDIIIDAYRIELLEPRQLGAGSGMSQYGWR
                     IGAAAAGALALVLAHRLGWAAAYVACAAFALPAMLVGVVMGEPARHREPARPHGAMEA
                     LVAYFSPLLEFLRRQGAIVVLVFVLIHKIGDTLANLTLRLLFQDLGFTNDEVAFYDVG
                     IGFGALLVGVFVGGLLYARLGMKGSVLVSLILMAVSNLSFAALAAAGHTNIGMAAAVG
                     FENFASGIGGVTVVAYLSALCNLRFTATQYALLSALASIAGRFLTGTSAGALIDAMGY
                     VNFYLVTTVIALPGVVLFWFMIRSGLADLSIGSAGKEGASP"
     gene            complement(5359..>5749)
                     /locus_tag="VFR57_02135"
     CDS             complement(5359..>5749)
                     /locus_tag="VFR57_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0258202.1"
                     /translation="FLVHLQRLRQRNQEIILCGDWNIAHKEVDLKNWRSNQKNSGFLP
                     EERAWLTRVFDELGCVDVFRRLNDQPDQYTWWSNRGQAWKKNVGWRIDYQIATPGIAA
                     LARRESIYRAKRFSDHAPLIIDYEHEL"
BASE COUNT         1062 a         1951 c         1810 g          926 t
ORIGIN      
        1 aacctggccg aggtgcaggc gctcgcgcag cgcgtgctgc gggcgagcga cccggtcaag
       61 acccgcgacc ttctcgccaa actcaacgcc tagaaaggtt tcaatgaaaa aagtcttcgc
      121 cctggggatc gtgcttttcg ccgcgtccgc cctcgcgcaa acctatcccg cgcgacccgt
      181 gaagctggtc gtccctttcc ccgcgggtag cgcgaccgac cagatcgccc gcgtgatgag
      241 ccagcagttc caagaagcgc tgggccagcc gttcgtggtc gagaacaaac ccggcgcgca
      301 gggctcaatc gcggccaccg aggtggcgcg cgccgcaccc gacggctaca cgatcatggt
      361 cactaccaac accccgcagg cggccaacgt cagcctgttc aagaagctca actacgaccc
      421 ggtgaaggac ttcgcgccga tcgcgcgcct tggaaccatc tcgttcatga tcatggtgcg
      481 gcccgatttc ccggcgaaaa atctcaagga attcctggcg cacgcgaaag ccaatcccgg
      541 gaagctctcc gccgggtacg gctcggcggg ctcgcaagtg tcgcaggcga tgctgcgctc
      601 catgggcggg atcgacttcg tcgacgtgcc gtacaaaggc ctgccgcagg ccatcaccga
      661 cgtcctcggc ggctcgatcc acttcacgtt cgccgacctc gccaacgcgc tcgcgcagat
      721 caagggaggg aagctccgcg ggctcgcggt tacctcgcgg aagcgctcgc cgcttgcgcc
      781 cgacgtgccg gcgattgccg aagagcttcc aggctacgag cttatcgcct ggttcgcgct
      841 cgtcgcgccc gccgacacac ccgctccggt ggtgacccgg ctgcacgacg tcaccgtgaa
      901 gtcgcttgcc aagccggagg tgaaggcacg gttcgactcg ctcggcaccg acgtggcgcc
      961 catgaatccg caggagctgg gcggtttcat ccgctcggag atcgagaagt gggccaagat
     1021 ggcgaaggcg gccggcatcc agccggagta gggaacaacc gcgtggcagg tccgctcgcc
     1081 ggcgttcggg tactcgacct cactacggtc gtgatgggtc cgtacgcaac ccagatcctc
     1141 ggcgacttcg gcgccgacgt gatcaaggtc gagccacccg agggggacgt gatgcgctat
     1201 gcctggccgt tccgcaaccc gggcatgggc tcgatctacc tcaacgtgaa ccgcaacaag
     1261 cgctcagtgg tgctcgatct caagaaagaa gcggcgcgcg aggcttgcct cctcctcgcg
     1321 cggcgcgcgg acgtgctcgt atacaacatc cgaccgcagg ccatggctcg cctcaagcta
     1381 tcctacgacg aggtcaggaa agtcaacccc cgcatcattt acgccggctg cttcggctac
     1441 agccagcgcg gaccctatgc cgcgaaggcc gcctacgacg acctgatcca gggcgcggcc
     1501 ggtctgccgt ggctgctgca aaagcagggc gcttccgagc cgcgctacgc gccgatgatc
     1561 gtcgccgatc gctccgtagg ccagcaggtc gccagcgcga tcaacgcggc cctctaccac
     1621 cgcgagaaga ccggcaaagg tcagcgcatc gacgtgccga tgttcgagca cttgctgcag
     1681 atcgtgctcg gcgatcatct cggcggctac accttcgacc cgcagtacgg cgagccgggc
     1741 tacgcgcgca tcctgacgcc ggatcgccgc ccctaccgga ccgccgacgg ctatgtctgc
     1801 gcgctcatct atacgaacaa gcagtggaaa gcatttctcg acatcatcgg taagccggag
     1861 atgctcgagc tgcccgagta cgcgacacag gaggcgcgca gccagaacta ccgccagtcc
     1921 tacgccatga tcgccgagga gatgaagcgc cgcaccacca cattctggct gcacgcgctc
     1981 gagcgcggcg atatcccagt gcagcgcatg aacaccctcg acgacatcgt tgccgacccc
     2041 cacttgagag cgatcgacta tctgcacatg gtggaccatc cgtccgaagg ccgcatccgg
     2101 gcgctcgccg tcccttctga gtggtcggaa tcgaagcccg agtaccggcg tcacgcgccc
     2161 cgcctcggcg agcacacgcg cgaggtgttg cgcgaagcgg gtctttcaga tcacgaaata
     2221 cattccctga tcgactcggg agcggccaaa caagctccgc tataattcgc ggcgcgccga
     2281 tttgatgatc agcttgcggg cgcctaacaa atgccgctaa agagatgaac gcacctcatc
     2341 acgatccctc ctcggtcggc accgcccagg cgcagaacgc ccgcttcagc gagccgctca
     2401 ggctgcgcgg cggcgcggag ctggccgatt tcgagatcgc gtacgagacc tacggccggc
     2461 tgaacgaggc gcgctcgaac gcagtgctgg tatgccacgc gctcaacgcc tcccaccatg
     2521 tggcggggtt ctacgagaac gatcctcaga acgtaggctg gtgggacaac ctcgtcggtc
     2581 ccggcaagcc gctcgacacc agccgcttct ttgtcgtcgg gtcgaactac atcggcagct
     2641 gcttcggctc gaccggaccg gcttcgatca atccggcgac cggtaagccg tggggcgcgg
     2701 atttcccggt ggttacggtg gaggactggg tcgaggcgca ggcccggctc gccgaccgcc
     2761 tgggcataga gcgcttcgcc gccgtgatcg gcggctcgct cggcgccatg caggcgcttc
     2821 agtggacgct ctcctacccc gggcgcatcc gacactcgat cgtgattgcc gcagcggcga
     2881 agctgacccc gcagaacatc gccttcaacg aagtcgcgcg ccaggcgatc atgaccgacc
     2941 ccgactttca tggcgggcat tactacgaga aaggcgtggt gccgacacgc ggcctgcgca
     3001 tcgcgcgcat gatcggccac atcacctacc tctcgggcga agcgatgagc gaaaagttcg
     3061 ggcggctcct gcgccgcggc gcgccgggct tcgacttcga catcgatttc gagatcgagt
     3121 cctacctgcg ctatcagggc gacaagttct cgagctattt cgacgccaac acgtatctgc
     3181 gcatcaccaa ggcgctcgac tacttcgatc cggcggccga ctacggcggc gacctgacca
     3241 aggcgctcgc ccgcgcgaag gcggcgttcc tcgtggtctc gttcaagtcc gactggcgct
     3301 tcccgccggc gcgctcgcgc gacatcgtgc gcgcgctgct cgacaaccgg cgcatcgtgt
     3361 cgtacctcga gctcgacgcg ccgggcgggc acgacgcctt cctgctcgag gattcgcgct
     3421 atcacacggc gctgcgccac tacttctcca atatcgagct gtgagatccg atcttcaggc
     3481 gatcgtgcaa tgggtgccgc agggcgcgcg ggtgctcgat ctcggctgcg gcgacggctc
     3541 gctcctgcgc tcgctgtggc aggagcgcca ggcgcccggc tacggcgtcg agatcgagga
     3601 ccaggcggtg cttgcttgcg tcgagaacga cgtcaacgtt ctgcaggtag acctcgaaag
     3661 cggcctttcg ctattttcgg atggttcgtt cgattgcgtg atcctttccg agacgctgca
     3721 gaccatccac cgcaccgagt tgctgctgcg cgagatgctg cgcgtgggtc gcgagggcat
     3781 cgtcagcttc ccgaacttcg gccattggag cgcgcgcctg cagatcgcgc tcggccgcat
     3841 gccggtctcc gagacccttc cctatcagtg gtacgaaaca cccaacgtcc accactgcac
     3901 cattacggat ttcgaagacc tgtgccgacg catcggcatc cggatccgcg agcgcctcgt
     3961 gcttcacgac ggcaagcccg ttacgatgct gcctaatctt ctcggcagcg tcgccgtgta
     4021 ccgctgcacg caaagctgaa gctctgcgtg aagcgcatct tctgccctta aggcgaagcg
     4081 ccttccttgc ccgccgagcc gatcgagaga tcggcgagcc cggagcggat catgaaccag
     4141 aagagcacca cgccgggcag cgcgatcacg gtcgtaacca gatagaaatt gacgtagccc
     4201 atggcatcga tcagcgcccc ggcgctcgtg ccggtgagga accggccggc gatgctagcg
     4261 agcgccgata gcagagcgta ctgcgttgca gtgaagcgca ggttgcacag cgccgacagg
     4321 taagcgacga cggtgacccc gccgatgccg ctcgcgaaat tctcgaaacc gactgcggcg
     4381 gccatgccga tgttggtgtg gcccgcggcc gccagagcgg cgaagctcag attcgatacc
     4441 gccatcagga tcaggctgac gaggaccgaa cccttcattc ccagccgcgc gtacaagagg
     4501 ccgccgacga acacgccgac gagcaaggcg ccgaagccga tgccgacgtc gtagaaggca
     4561 acctcgtcgt tggtaaagcc gaggtcctgg aacagcagcc gcagcgtgag attggcgagc
     4621 gtgtcgccga tcttgtggat gagcacgaag acgagcacca cgatcgcgcc ctgacgcctg
     4681 aggaactcca agagcgggct gaagtacgcg acgagcgctt ccatggcgcc gtgcggtctg
     4741 gcgggctcgc ggtggcgcgc cggctctccc atgacgacgc cgaccagcat cgccggaaga
     4801 gcgaacgccg cgcacgcgac gtaggccgcc gcccacccca gacggtgagc gagaacgagc
     4861 gccagcgcgc cggcggcggc cgcgccgatg cgccagccgt attgcgacat gcccgagccc
     4921 gcgccgagct gtcgcggctc gagcagttcg atgcggtagg catcgatgat gatgtcgaag
     4981 gtcgcaccag ccacgccgac caggatggcc gcgatcgcca ccatatagag cgcctcttgt
     5041 ggatcggcga cgccgagaac agcgaccgcc gccatgacga gtacgccgac gagccatagc
     5101 cagctgcgcc gctgtccgaa ccggccgatc aggggcaggc gcacgttgtc gacgatcggc
     5161 gcccacagga acttgaagtt gtaggcgaga aaggtgagcg cgaaagctgt caccgcgctc
     5221 ctcgtaatgc cgtgctgcgc gaggcgcgtg ctgagcgtcg cgccgatcat cgcgaacgtg
     5281 aagcccgatg aaactccgag taaaagcgcc gcgagcggcg ccggctccag atacagccgc
     5341 aggcccgccg gcagcctgct atagctcatg ctcgtagtcg atgataagcg gcgcgtggtc
     5401 ggagaagcgc ttcgccctgt agatcgattc cctgcgcgcg agcgccgcga tgccgggcgt
     5461 cgcgatctgg tagtcgatgc gccagcccac gttcttcttc caggcctggc cgcggttcga
     5521 ccaccaggtg tactggtcgg gctggtcgtt cagcctgcgg aacacgtcga cgcagccgag
     5581 ctcgtcgaac acgcgcgtga gccacgcacg ctcctcgggc agaaagcccg aattcttctg
     5641 gttcgagcgc cagttcttga ggtcgacctc cttgtgcgcg atgttccagt cgccgcacag
     5701 gatgatttcc tggttgcgct gtctcaaccg ctgcagatga accaggaag
//
LOCUS       DASTPD010000053         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1249970, whole genome shotgun sequence.
ACCESSION   DASTPD010000053 DASTPD010000000
VERSION     DASTPD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249970"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFR57_02140"
     CDS             <1..469
                     /locus_tag="VFR57_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0258203.1"
                     /translation="AVSAPVANYVNAVRTGNLLYLAGKGPRPGRDGRRPKGKVGRDYT
                     VEEAYQHARTVGLDLLAVMRQELGSLDKVKRVVKVLGMVNASPEFEDHPKVINGCSDL
                     FVQVLGDKGKHARSAVGMGSLPMGIPVEIEVIVEVENRGQSPISPSRKGKSRK"
     gene            complement(499..1041)
                     /gene="orn"
                     /locus_tag="VFR57_02145"
     CDS             complement(499..1041)
                     /gene="orn"
                     /locus_tag="VFR57_02145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEU0258204.1"
                     /translation="MAADPNNLIWIDMEMTGLQPDADRVIEIAMLVTDPQLNVLATGP
                     VLVLHQPDEVLDAMDTWNKSTHAKTGLIERVRASSLTEADAERQALEFVSAHVPANVS
                     PMCGNSICQDRRFLARWMPGLEAHFHYRNLDVSTLKELVRRWKPELKAFSKEGKHEAL
                     ADILESIEELKFYRKSVMTI"
     gene            1060..2304
                     /locus_tag="VFR57_02150"
     CDS             1060..2304
                     /locus_tag="VFR57_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0258205.1"
                     /translation="MNVAFTWVFIAAVALATATRLWLTLRQIRHVRANRNAVPGMFAD
                     SIPLSAHQKAADYTVAKAKLGVVDTLLDALILLALTLGGALQWLSDLWQRVFALESLW
                     HGAALILSMLVIKGALGLPLSLYRIFVIEERFGFNRMTWKLFVTDLVKGLAVGMVLGV
                     PLLLAVLWLMLAAGTFWWLYVWLVLVVYSLFLQMIYPALVMPLFNKFSRLDDPALAGR
                     IERLLSRTGFRSRGLFVMDGSKRSSHGNAFFAGFGAAKRIVLFDTLVSRLQPAEVEAV
                     LAHELGHYKLRHIVKGMALSWGFSFALLLALGLIAGEPWFYEGLGMDTATLPIALALF
                     LLVVPEFTFFVQPLLSLFSRKNEYEADRYAAANADAAQLVRALVKLYHDNSATLTPDP
                     LHSAYYDSHPPAAMRIARLRTT"
     gene            2301..2630
                     /locus_tag="VFR57_02155"
     CDS             2301..2630
                     /locus_tag="VFR57_02155"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU0258206.1"
                     /translation="MNELAKKRCKPCEGGVKPFTPQEASQLLKTLEGWAIENGRLAKT
                     FAFQNYYETMAFVNALAWISHREDHHPDLGVHSNKCRVEYWTHAIGGLSENDFICAAK
                     ADALAET"
     gene            2627..2923
                     /locus_tag="VFR57_02160"
     CDS             2627..2923
                     /locus_tag="VFR57_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU0258207.1"
                     /translation="MKRVFSSYNQTAVYHARNLLQNAGIETLVRNAILSSAMGELPPA
                     ECQAELWVLDDSDERRAQEILSQQVTGADWICTCGETLGAQFTQCWRCGAYRSA"
     gene            complement(2908..>3260)
                     /locus_tag="VFR57_02165"
     CDS             complement(2908..>3260)
                     /locus_tag="VFR57_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEU0258208.1"
                     /translation="QDWFGFERNPYDRIGHFMQGFVPAILAREILLRTHSVRTKKMAA
                     FLSVCVALAFSAFYELIEWWAALALGQGAHEFLGTQGDPWDTQADMFLALIGAGSALV
                     LLSRIHDRQVQALR"
BASE COUNT          556 a         1066 c         1036 g          602 t
ORIGIN      
        1 cgccgtgtcg gccccggtcg cgaactatgt caacgcggtg cgtactggaa atctcctcta
       61 cctcgccggc aagggcccgc gccccggccg ggacggccgg cgtccgaagg gcaaggtcgg
      121 ccgcgactac acggtcgagg aggcgtacca gcacgcgcgc acggtaggac tcgatctgct
      181 cgcagtcatg cggcaggagc tcggtagcct cgacaaggtg aagcgcgtgg tgaaagtgct
      241 cggcatggtc aacgcgtcgc ctgagttcga ggaccatccg aaagtgatca acggctgctc
      301 cgacctcttc gtccaggtgc tcggcgacaa gggaaagcac gcgcgctccg cggtcggcat
      361 gggttcgttg ccgatgggca tcccggtcga gatcgaggtg atcgtcgagg tggaaaatcg
      421 gggtcagagc ccgatttctc cgagccgcaa ggggaaaagt cggaagtaga agaaatcggg
      481 ctcggacccc gatcttcctc agattgtcat cacgctcttg cggtagaact tgagctcctc
      541 gatcgactcg aggatgtcgg cgagcgcctc gtgcttgcct tccttcgaga acgccttcag
      601 ctccggcttc cagcgccgca ccagctcctt cagcgtcgag acgtcgagat tgcggtagtg
      661 gaagtgcgct tccaggccgg gcatccagcg cgcgagaaag cggcggtcct ggcagatcga
      721 attgccgcac atcggcgaga cgttcgccgg cacgtgcgcg gagacgaact cgagcgcctg
      781 acgctcggcg tcggcctcgg tcaggctcga ggcgcgcacg cgctcgatta atcctgtctt
      841 cgcgtgcgtg ctcttgttcc acgtatccat cgcatcgagc acctcgtcgg gctggtggag
      901 gacgagcacc gggccggtcg cgagcacgtt gagctgggga tcggtgacca gcatcgcgat
      961 ttcaatgacg cggtccgcat cgggctgcaa cccggtcatt tccatgtcga tccatattag
     1021 gttgttcgga tccgcagcca tggcttattc tctcatggca tgaatgtcgc ctttacctgg
     1081 gtattcatcg ccgcggtcgc cctcgcgacg gcgacgcggc tatggctcac gctgcgccag
     1141 atacggcacg tgcgcgcgaa tcgcaacgcg gtgcccggca tgttcgccga ttcgatcccg
     1201 ctctccgcgc accagaaggc cgccgactac accgtggcga aagcaaagct cggcgtcgtg
     1261 gacacgctgc tcgatgcgct tatccttctc gcgctcacgc tgggcggcgc tctgcagtgg
     1321 ctgtcagacc tttggcagcg cgtgttcgcg ctcgaatccc tctggcacgg cgcggcgctg
     1381 atcctgtcga tgctggtgat caaaggcgcg ctcggcctgc cgctcagcct gtatcgcatc
     1441 ttcgtcatcg aggagcgttt cggcttcaac cgcatgacgt ggaagctctt cgtcaccgac
     1501 ctggtcaagg gactcgcggt cggcatggtg ctcggcgtgc cgctgcttct cgccgtgctc
     1561 tggctcatgc tggcggcggg gacgttctgg tggctctatg tctggctcgt gctggtggtc
     1621 tactcgcttt tcctgcagat gatctacccg gcgctggtca tgccgctttt caacaagttc
     1681 tcgcggctcg acgacccggc gctcgccggc aggatcgagc ggctgctcag ccgcaccggc
     1741 ttccgctcgc gcggcctgtt tgtcatggac ggctcaaaac gctcgtctca cggcaacgcg
     1801 ttcttcgccg gatttggcgc cgcgaagcgc atcgtgctgt tcgacaccct ggtgtcgcgc
     1861 ctgcagcccg ccgaggtcga ggcggtgctc gcgcacgagc tgggccacta caagctgcgt
     1921 cacatcgtca aaggcatggc gctcagctgg ggattcagct tcgccctgct cctcgccctt
     1981 gggctgatcg cgggcgagcc gtggttctac gaaggccttg gcatggacac cgcgaccctg
     2041 ccgatcgcgc tcgcgctctt cctgctggtg gtgccggaat tcacgttctt cgtacagccg
     2101 ctgctctcac ttttctcgcg caagaacgag tacgaggccg accgctacgc cgccgccaac
     2161 gccgacgcgg cgcagcttgt gcgcgcgctc gtcaagctct accacgacaa ctcggcgacc
     2221 cttacgccgg acccgctgca ctctgcctac tacgattcgc acccgccggc cgcgatgcgg
     2281 atcgcccgtc tgaggaccac atgaacgaac tcgcgaagaa acgctgcaag ccctgcgaag
     2341 gcggcgtgaa gccgttcacg ccgcaggaag cgagccagct cctgaagacg ctcgagggct
     2401 gggcgatcga gaacggcagg cttgccaaga cctttgcgtt ccagaactac tatgagacga
     2461 tggcgttcgt gaacgcgctc gcgtggatct cgcaccgcga ggaccaccat cccgacctcg
     2521 gcgtgcactc caacaagtgc cgcgtcgagt actggacgca cgcgatcggc gggctgtcgg
     2581 aaaacgattt catctgcgcg gccaaggccg acgcgctcgc cgagacttga agagagtctt
     2641 cagctcgtac aaccagaccg cggtctatca cgcccgcaac ctgctgcaga acgcgggcat
     2701 cgagactctc gtaaggaatg ccattctttc ttcggccatg ggtgagctgc cgccggccga
     2761 atgccaggcc gagctttggg tactcgatga ttcggacgag cggcgcgcac aggaaatcct
     2821 gtcccagcag gtgacgggcg cggactggat ctgcacctgc ggcgagacgc tcggcgctca
     2881 gttcacgcaa tgctggcgct gcggcgccta ccgcagcgcc tgaacttgcc ggtcgtggat
     2941 ccgcgatagc agcacgagcg cgctgccggc gccgatcaaa gcaaggaaca tgtcggcctg
     3001 cgtgtcccac ggatcgccct gagtgccgag gaactcgtgc gccccctgcc caagcgcgag
     3061 cgccgcccac cattcgatca gctcgtaaaa ggcgctgaag gcgagcgcaa cgcacaccga
     3121 gaggaacgcg gccatcttct tcgtccttac ggagtgcgtg cgaagcagga tctcgcgcgc
     3181 gaggatcgcc gggacgaagc cttgcatgaa atggccgatg cggtcgtagg gattgcgctc
     3241 gaagccgaac cagtcctgca
//
LOCUS       DASTPD010000054         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_842737, whole genome shotgun sequence.
ACCESSION   DASTPD010000054 DASTPD010000000
VERSION     DASTPD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842737"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFR57_02170"
     CDS             <1..1401
                     /locus_tag="VFR57_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258209.1"
                     /translation="DPTMARTFVGRIVFAGLCDKLFDLDEKLNIVPQLATSYKWSADN
                     KSLEIKLREGVTFHDGEKLDAAAVKYNLERHKNMKGSSRRGELAVVSSVDAVDASSVR
                     INLAAPFAPLLAVLTDRAGMMVSPKAAEAAGDKFGAKPVCSGPFRFVERIAQDRIVLE
                     RFPNYWNKGEIHFDRIVYQPIVDATVRLANLRSGQLDFIERVAPSDIPQLRNDNRFRI
                     AKIVEIGYQGITINVGKSDLAQKNPLGKDPRVREALELSLDRDAIVKVAMEGEAQPGN
                     QWVAPTNRFYGKSAPIPKRNVERAKQLLKEAGVPNPSFTLMTPTTSDAQRIAQIVQAM
                     AKEAGFDVKIHSTEFATSLNLADKGQFEAYVLAWSGRADPDGNLHTMLACKGPTNYAG
                     YCKEDVEKLIDESRTSLDATRRIAAYDKIAQAVQKDRPIIYLYHRNWLWAHTAKLTGL
                     RTVPDGMVRVQGLKMN"
     gene            1403..2344
                     /locus_tag="VFR57_02175"
     CDS             1403..2344
                     /locus_tag="VFR57_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258210.1"
                     /translation="MGKFLLQRLATIVPTLFFVSVLIFGLQQLLPGDPAIALAGEERD
                     PETIRYLREKFHLDDPLPVRYWHWASGVLQGDLGDSVRIQKPVMDLIVEKLPVTIQLA
                     TMAMLIALAIGITAGVISAVKKDSWLDYAANVFALWGLSTPNFWLGILLILLFAVQLG
                     WLPASGYVSPFEDLKANLSAMIMPAFVLGNAIAAVLMRHTRSAMLQVLSSDYVRTARA
                     KGLDERVVVLKHALRNALIPIITLGALEFGTLLSGAVLTEQVFTIPGFGKLIVDAVFN
                     RDYLVVQGVVLFTATVYITLNLLADVAYFVVNPRMRH"
     gene            2344..>2708
                     /locus_tag="VFR57_02180"
     CDS             2344..>2708
                     /locus_tag="VFR57_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258211.1"
                     /translation="MAAVVFQEIETTPGRKTWKRLLRRGGAMFGLAVVGFFVLIAILA
                     PWLAPHDPLGTSWSAVRAAPSAAHWFGADELGRDVLSRIIWGTRASILAGVVSVSISL
                     ALGVPIGMAAGYLGRWVDAL"
BASE COUNT          480 a          896 c          863 g          469 t
ORIGIN      
        1 gatcccacga tggcgcgcac gttcgtgggc cgcatcgtgt tcgccggctt gtgcgacaag
       61 ctcttcgacc tcgatgagaa gctgaacatc gtgccgcagc tcgccacctc ctataaatgg
      121 tcggccgaca acaagtcgct cgagatcaag ctgcgcgaag gggtcacgtt ccacgacggg
      181 gagaagctcg atgccgccgc ggtcaagtac aacctcgagc gccacaagaa catgaagggg
      241 tcgagccgcc gcggcgagct cgccgtggtg tcgagcgtgg acgcggtcga cgcctcgagc
      301 gtgcgcatca acctcgccgc gccgtttgcg ccgctgctcg cggttctcac cgaccgcgcc
      361 ggcatgatgg tgtcgcccaa agccgccgag gcggcgggcg acaagttcgg cgccaagccc
      421 gtctgctccg ggccgttccg cttcgtcgag cgcatcgcgc aggaccgcat cgtgctcgag
      481 cgcttcccga actactggaa caagggcgag atccacttcg accgcatcgt ctaccagccg
      541 atcgtcgacg caaccgtgcg gcttgccaac ctgcgctcgg gtcagctcga cttcatcgag
      601 cgcgtcgcgc cttcggacat cccgcagctt cgcaacgaca atcgcttccg gatcgcgaag
      661 atcgtggaga tcggctacca gggcatcacc atcaacgtcg gcaagagcga tcttgcgcag
      721 aaaaacccgc tcggcaagga cccgcgggtg cgcgaggcgc tcgagctttc gcttgaccgc
      781 gacgcaatcg tgaaggtcgc catggagggc gaggctcagc ccggcaacca gtgggttgcg
      841 cccaccaacc gcttctacgg caagagcgcg ccgatcccga agcgaaacgt ggagcgcgcg
      901 aagcagcttc tcaaggaagc cggggtgccg aacccgagct tcacgttgat gactccgacc
      961 acctcggacg cgcagcgcat cgcgcagatc gtgcaggcga tggccaagga agcgggcttc
     1021 gacgtcaaga tccactccac tgagttcgcc acatcgctca acctcgccga caaggggcag
     1081 ttcgaggctt acgtgctcgc gtggagcggg cgcgccgacc ctgacggcaa cctgcacacc
     1141 atgcttgcgt gcaagggtcc cacgaactat gccggctact gcaaggagga cgtcgagaag
     1201 ctgatcgacg agtcgcgcac ctcgctcgac gcgacgcgcc gcatcgcggc ctacgacaag
     1261 atcgctcaag ctgtgcagaa agaccgaccg atcatctacc tctaccatcg caactggctg
     1321 tgggcacaca ccgccaagct caccggactg cgcaccgtcc ccgacggcat ggtgcgggtg
     1381 cagggcttaa agatgaattg agatggggaa gttcctgctg cagcgcctgg cgacgatcgt
     1441 gccgacgctg ttcttcgtat cggtgctgat cttcgggctg cagcagctcc tccccggcga
     1501 cccggcgatc gcgctcgcgg gagaagagcg cgacccggaa acgatccggt atctaagaga
     1561 gaagttccat ctcgacgatc cactgccggt gcgctactgg cactgggcat cgggggtgct
     1621 gcagggcgac ctgggcgact cggtgcgcat ccagaagccc gtgatggacc tgatcgtcga
     1681 gaagctgccg gtcaccatac agctcgccac gatggcgatg ctgatcgcgc tcgcaatcgg
     1741 catcaccgcc ggcgtgatct ccgcggtgaa gaaggacagc tggctcgact acgccgccaa
     1801 cgtgttcgcg ctgtggggcc tctccacccc caacttctgg ctcggcatcc tgctcatcct
     1861 gctcttcgcg gtgcagctcg gctggctgcc cgcctcgggg tacgtgagcc cgttcgagga
     1921 cctgaaagcg aacttatcgg cgatgatcat gccggcgttc gtgctgggaa acgcaatcgc
     1981 cgcggtgctg atgcgccata cgcgcagcgc catgcttcag gttttgagca gtgattatgt
     2041 ccgtactgca cgggcaaaag gactggacga acgggtcgtg gtgctgaagc acgcactgcg
     2101 caacgcgctc atcccgatca tcacgcttgg cgcacttgag ttcggcacgc tgctttcggg
     2161 tgcggtgctc acggaacagg tgttcaccat cccgggcttc ggcaagctca tcgtggatgc
     2221 agtgttcaac cgcgactacc tcgtcgtgca gggcgtggtg cttttcaccg cgacggtgta
     2281 catcacgctc aacctgctcg ccgacgtcgc gtatttcgtc gtcaatccgc ggatgcgtca
     2341 ctgatggcgg ccgttgtctt ccaggaaata gaaaccacac cgggtcgaaa gacctggaaa
     2401 cgcctgctgc gccgaggagg cgcaatgttc ggcctcgcgg tggtggggtt cttcgtcctc
     2461 atcgcgatcc tcgcgccctg gctcgcgccg cacgacccgc tcggcacgag ctggagcgcg
     2521 gtgcgtgcgg cgccgagcgc ggcgcactgg ttcggcgccg acgagctcgg gcgcgacgtg
     2581 ctctcgcgca tcatctgggg cacgcgtgcc tcgatcctcg ccggcgtggt gtcggtctcg
     2641 atctcgctcg cgctcggcgt gccgatcggc atggccgccg gctacctcgg gcgctgggtc
     2701 gatgcgct
//
LOCUS       DASTPD010000055         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1250922, whole genome shotgun sequence.
ACCESSION   DASTPD010000055 DASTPD010000000
VERSION     DASTPD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250922"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFR57_02185"
     CDS             <1..201
                     /locus_tag="VFR57_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106484.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HEU0258212.1"
                     /translation="PGGLLATFSCSGGVSTELFQSIVAGAALDARADAKIIERFTAGA
                     DHPVALEFPEGEYLKGLLLLRS"
     gene            249..1553
                     /locus_tag="VFR57_02190"
     CDS             249..1553
                     /locus_tag="VFR57_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258213.1"
                     /translation="MSMRRKALAASVVLTLLGCAGEPLAPRSDTVSVATLGAQAGKPI
                     KREMLRAEVMRVADRYAVTMAQEADRIRDAEGGERFGYFATGWKLGTRTAALQIAMGE
                     NAVENLLDMLVLTSLTRHSVESYWSPKFLGGSRGDGLLAASRAFEDQIWKASGRVLTP
                     GQQESLRALIEEWIAKNPDHAYVWEVRFSGFSGQRAEALERVSATGGLLAEAQRAMET
                     VDEVRVLSERMAYYLLRAPTIARLQAELGVYNVLETAQINRLMDDLHRTSGAVEQFGV
                     LAKQLPAEREAAITQLLREFGREREAAIMQLAKEVTREREASIVQASQELSREREAAV
                     RQVMLEQRQTVVALLKSQELAALVDRASVQSEDIINAAFVRGALLILLWMVAYLVVRM
                     VSGYWVGRMGDRKAPTFGSIAVGRGGTQVPVGEAVARRGEKR"
     gene            1647..2627
                     /locus_tag="VFR57_02195"
     CDS             1647..2627
                     /locus_tag="VFR57_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0258214.1"
                     /translation="MQEQDMVPVTILTGFLGSGKTTLLNRILKEDHGHRLAVIENEFG
                     EVGVDNDIIELGDEQIVEMNNGCICCTVRGDLIRILGSLKEKRDQGNLKFDRVVIETT
                     GMADPGPVAQTFFTDEEIGNYYLLDSIITLVDAKHAPKQLDEFHEAQEQVGFADRILL
                     SKTDLTKEDDTEVLIKRLKRMNPRAPIRKVHFGQAKIEEILDIRGFNLNAILELDPEF
                     LTNIAHDHHDEVESFVFRADKPFDGQKLESFLSGMIQVYGPDLLRYKGILWMKGNNRR
                     VVFQGVHMMMGGDMGKPWGKGEKKSSLLVFIGKKLPKDIFIAGLEECLAK"
     gene            2628..3254
                     /locus_tag="VFR57_02200"
     CDS             2628..3254
                     /locus_tag="VFR57_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0258215.1"
                     /translation="MISVRGLAHRYGAVEALRLAEWNVAQGERWLALGPSGCGKTTLL
                     HIIAGLVRPSEGRVEVSGENLGRLDAARLDRWRGTTVGIVLQALHLVKHLSVRDNLRL
                     AQYLAQVPQDDNRIADTLGALGVAEKGACKPSELSQGEQQRVAIARAVVNRPKLLLAD
                     EPTANLDDAAAAKVVELLSEQAARHGATLVVATHDARVKGKFRERLEL"
     gene            3251..>4176
                     /locus_tag="VFR57_02205"
     CDS             3251..>4176
                     /locus_tag="VFR57_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258216.1"
                     /translation="MNLTTLSASYLRARPLNTVLSLVLLSLGVATIVLLLLVVSQLEE
                     RMHRDARGIDLVVGAKGSPMQLILSGIYHVDAPTGNIPLSSVEMLSSNKMVKRAIALA
                     LGDSWKGYRIVGAPRAYPEHYGATLESGRLHDKPLEAVLGAEVAARTGVAVGGTFTGA
                     HGIGAEGGAEHAEAPYTVVGMLNRTNSVLDRLVLTSIESVWQVHEEHQGPEDEADRKA
                     LEEAREVTVVLVQYASPLAAATLPRQINAQSELQAASPAYETARLFRIVGVGIEALRA
                     FALVLIAAAGLSVFIALYNALEERRYDLAVMRT"
BASE COUNT          751 a         1310 c         1428 g          687 t
ORIGIN      
        1 cccggcgggc tgctcgccac cttctcctgc tccggcggtg tgtcaaccga gctcttccag
       61 tcgatcgtcg ccggcgcggc cctcgacgct cgcgcagacg cgaagattat cgagcgcttc
      121 accgccgggg ccgaccaccc agtggcgctc gagttcccag agggcgagta cctgaagggg
      181 ctgctgctgc tgcgcagtta gttgatcttc atcaaatggc cgcggggcgt gccccatagc
      241 atcccggcat gagcatgcgc cgcaaagcgc tcgccgcctc ggtggtgctg acgctactcg
      301 gctgcgcggg cgagccgctt gcaccaagga gcgacacggt gtccgtggcg acgctcggcg
      361 cgcaggccgg gaagccgatc aagcgcgaga tgttgcgcgc cgaggtgatg cgcgtcgcgg
      421 accggtacgc cgtgaccatg gcgcaagagg ccgaccggat ccgggacgcg gaggggggcg
      481 agcgtttcgg ctatttcgcg actggatgga agctcggcac ccggaccgcg gcgctgcaga
      541 tcgcgatggg ggagaacgcc gtagagaacc ttctggacat gctggttctc acctcgctca
      601 cgcgccattc ggtcgaaagc tactggtcac cgaagttcct gggcggctcg cgcggagacg
      661 gcttgcttgc ggcttcgcgc gccttcgagg atcagatctg gaaagcttcc gggcgcgtgc
      721 tgacacccgg gcagcaggaa agcctgcgtg cgctgatcga agagtggatc gcgaagaacc
      781 cggatcacgc ttacgtgtgg gaggttcgct tctccggctt ctcgggccag cgcgcagaag
      841 cgctcgagcg cgtgagcgcc acgggcggcc tgctcgccga agcccagcgc gccatggaaa
      901 cggtggacga ggtgcgggtg ttgagcgagc gcatggcgta ctacctgctg cgcgcaccca
      961 ccatcgcgcg ccttcaggcc gagctaggtg tgtacaacgt tctggagacc gcgcagatca
     1021 accggctgat ggatgacctg catcgcactt cgggggcggt ggagcagttc ggcgtgctcg
     1081 ccaagcagct gccggcggag cgcgaggcgg cgatcaccca gctgctacgc gagttcggac
     1141 gcgagcgcga ggcggcgatc atgcagctgg ccaaggaagt cacgcgtgaa cgcgaggcga
     1201 gcatcgtcca ggcgagccag gagctaagcc gagagcgcga agctgcggtg cggcaagtga
     1261 tgctggagca gcggcagacg gtagtcgcgc tcctgaagtc ccaagagctt gccgccttgg
     1321 tcgatcgggc aagcgtgcag agcgaagaca tcatcaacgc cgcctttgtg cgcggtgctc
     1381 tgctcatcct gctctggatg gttgcctatc tcgtcgttcg catggtgagc gggtactggg
     1441 tcgggaggat gggtgaccgg aaagccccaa ccttcggttc tatcgcggtc ggtaggggcg
     1501 gaacccaagt ccccgttggc gaagccgtcg ctcggcgcgg tgagaaacgg tagaacgcag
     1561 tcgaagtagg tcgggctcga cctcatccgt gcgcggcacc tcgccccggc gctggattaa
     1621 aatgccgctc cttccgggag cttcgcatgc aagagcagga catggtgccg gtcacgatcc
     1681 tcaccggctt cctcggcagc ggcaagacca cgttgctcaa ccgtatcctc aaggaggatc
     1741 atgggcaccg cctcgcggtg attgagaacg agttcggcga ggtcggcgtc gacaacgaca
     1801 tcatcgagtt gggcgacgag caaatcgtcg agatgaataa cggctgtatc tgctgcacgg
     1861 tgcgcggcga cctcattcgc atcctcggct cgctcaagga gaagcgcgac cagggcaacc
     1921 tgaagttcga ccgcgtggtg atcgagacga cgggcatggc cgacccaggg ccggtggcgc
     1981 agaccttctt caccgacgag gagatcggca actactacct gctcgactcg atcattaccc
     2041 tggtggacgc gaagcacgcg ccgaagcagc tcgacgagtt ccacgaggcg caggagcagg
     2101 tcggcttcgc cgaccggatc ctgctctcga aaacggactt gacgaaggaa gacgacaccg
     2161 aggttttgat aaaacggctg aagcgcatga atccccgcgc gccgatccgc aaggttcatt
     2221 ttggacaggc gaaaatcgag gagatcctcg acatccgcgg cttcaacctg aacgcgatcc
     2281 tcgagctcga ccccgagttt ctcaccaaca tcgcgcacga ccatcacgac gaggtcgagt
     2341 cgtttgtgtt ccgcgccgac aagccgttcg acggccagaa gctcgagagc ttcttgtcgg
     2401 ggatgatcca ggtctacggc ccggatctcc tgcgctacaa aggcattctg tggatgaagg
     2461 gcaacaaccg ccgcgtcgtg ttccagggcg tgcacatgat gatggggggc gacatgggca
     2521 agccttgggg taagggtgag aagaagagct cgctcctcgt cttcatcggc aagaagctgc
     2581 ccaaggacat cttcatcgcc ggtctcgagg agtgcctggc taaataaatg atctcggttc
     2641 gcggcctggc gcaccgctac ggcgccgtcg aggcgctgcg gcttgccgaa tggaacgtgg
     2701 cgcagggcga gcgctggctc gcgctcgggc cctcgggctg cggcaagacg acgctgctgc
     2761 atatcattgc gggcctggtg cgcccgagcg agggccgggt ggaagtctcg ggagagaacc
     2821 tcggacgcct ggacgcggcg cggctcgatc gctggcgcgg aacgaccgtc gggatcgtgc
     2881 tgcaggcgct gcacctcgtg aagcatctca gcgtgcgcga caatctgcgg ctagcgcagt
     2941 acctcgcgca ggtgccgcaa gacgataacc gcatcgccga tacgctcggc gctctcggcg
     3001 tcgccgaaaa aggcgcgtgc aagccgagcg aactgtcgca gggcgagcag cagcgcgtcg
     3061 cgatcgcgcg cgccgtggtc aaccgtccga agctgctgct cgccgacgag ccgaccgcca
     3121 acctcgacga cgcggcggcg gcgaaggtgg tggagctgct ttccgaacag gcggcgcgcc
     3181 acggcgcgac gctcgttgtt gccacgcacg acgcgcgcgt gaaggggaag ttcagagaaa
     3241 ggctcgagct gtgaacctca cgacgctttc ggcgagctac ctgcgtgcgc gcccgctcaa
     3301 caccgtgctg agcctcgtgc tgctctcgct cggagttgcg acgatcgtgt tgctccttct
     3361 ggtagtaagc cagctggaag agcgcatgca ccgcgacgcg cgcggcatcg atctcgtcgt
     3421 cggcgccaag ggcagcccga tgcagctcat cctttcgggc atctaccatg tcgacgcgcc
     3481 gaccgggaat atcccgcttt catcggtcga gatgctttcc agcaacaaga tggtgaaacg
     3541 cgcgattgcg ctcgcgctcg gcgacagctg gaagggctac cgcatcgttg gtgctccgcg
     3601 cgcgtatccc gagcactacg gcgcgacgct cgagtcgggc cgactccacg acaagccgct
     3661 ggaggccgtg cttggcgcgg aagtcgcggc gcgcaccggc gtagcggtcg gcggcacctt
     3721 caccggcgcg cacggcatcg gagccgaggg cggcgcggag catgccgaag cgccctacac
     3781 ggtcgtcggc atgctcaatc gcacgaactc ggtgctcgac cggctggtgc tcacgagcat
     3841 tgaaagcgtt tggcaagtgc acgaggaaca tcagggccct gaggacgagg ccgaccgcaa
     3901 ggcgctcgag gaagcgcgcg aggttaccgt cgttctcgtc cagtacgctt cgccgctcgc
     3961 ggccgccacg ctgccgcgcc agatcaatgc gcagagcgag ctgcaagcgg cctcgccagc
     4021 gtacgagacc gcgcgccttt tccgcatcgt cggcgtcgga atcgaagcgt tgcgagcgtt
     4081 cgcgctggtg ctgatcgccg ccgcggggct gtcggttttt attgcgcttt acaacgcgct
     4141 cgaggagcgc cgctacgatc tcgccgtgat gcgcac
//
LOCUS       DASTPD010000056        12824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1659598, whole genome shotgun sequence.
ACCESSION   DASTPD010000056 DASTPD010000000
VERSION     DASTPD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659598"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFR57_02210"
     CDS             complement(<1..305)
                     /locus_tag="VFR57_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258217.1"
                     /translation="MSLGVLGIAALLAGAPWAQSQQAPVREITKIAGEVYRFRHANHY
                     SVFAVTPAGVIATDPINADAARWLEAEITQRFNQPVRYVVYSHDHADHIAGGEVFADT
                     "
     gene            complement(354..1313)
                     /locus_tag="VFR57_02215"
     CDS             complement(354..1313)
                     /locus_tag="VFR57_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0258218.1"
                     /translation="MKRVVAFAAVAAAFAGAPSAQAQQKFMTIGTGGVTGVYYAAGGA
                     ICRLVNKDRAKHGIRCSVESTGGSVFNINTIKAGELDLGVAQSDVQYNAAKGEAQYKD
                     GPFKELRAVMSLHPEPFTVLARKEANVKSFADFKGKRFNVGNPGSGTRASMEELLGAM
                     GWKMSDFSLAAELKADEHGPALCDGKIDGFFYGVGHPSANIQEPTTACGAKLVPVRGP
                     AVDKLVKGNPFYAYATIPGGIYRNNPEPTKTYGVLATLVTSTTMPASVVYQVVKAVFD
                     NFDEFRKLHPAFAYLETKDMVKNGLSAPLHEGAARYYKEKGWQ"
     gene            complement(1310..2644)
                     /locus_tag="VFR57_02220"
     CDS             complement(1310..2644)
                     /locus_tag="VFR57_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0258219.1"
                     /translation="MFDAVTHVFHRDPRLTYRVAVRGEGAYLIDRDGKRYLDASGGAA
                     VSCLGHSDAAVIQAIRRQLEKLPFAHTSFFTNEPMEELADALIARAPKSFDKVYFVSG
                     GSEAIEAALKLARQYFVEKGEPQRAHVIGRRQSYHGNTLGALAVGGNAWRRKQFEPLL
                     VRTTHVSPCYAYRGKNPGESDPAYVERLSEELEAQFQQKSVIAFVAETVAGATIGAVP
                     PVPGYFKRVRDLCDRYGVLLILDEVMCGMGRCGTLWTFEQEGIVPDLVAIAKGLGAGY
                     QPIGALLVSKEIADTIQRGTGFFQHGHTYIGHAAACAGALAVQKRLHEDGVMKKVFPL
                     GTALERRLREAFAGHPHVGDIRGRGLFWGIEIVEDRAAKKPFDPKLRMHARLKRKALE
                     AGLMCYPMGGTIDGVQGDHVLLAPPFIVEESQLDELVDKLGTAISTLEEQTI"
     gene            2709..3668
                     /locus_tag="VFR57_02225"
     CDS             2709..3668
                     /locus_tag="VFR57_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258220.1"
                     /translation="MFRILAVAAAAALLSGAATAQNKIKYALGDVIGIDDLPLLVAAE
                     RSKKRGVEVEITPFKNEDIATQAVINGQADIGQGTPYAAIEKVTVPIRFFVQLWSLQF
                     FPVVANEHYKSWKDLDGQEIAVQGRGSGTEAIMLLMAKQHGIKYKNVSYVPGSQVRAL
                     ALIKGNIRASILDATNKNFLMREAPGKFTILPLGEIKASDESLFARLEYLQKNQAAVQ
                     VFVEELVRVNREINKNPMSIVEERKKLGLLKDLPAKLEEELPTYFKEGVAGGIFPNDG
                     GAENAAKTDLEFFALSGSIKKENPKVDDYWHLAPLKAALSRLK"
     gene            3673..4395
                     /locus_tag="VFR57_02230"
     CDS             3673..4395
                     /locus_tag="VFR57_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258221.1"
                     /translation="MRGWQLASFALFFGLWELAGRWPVSAAFPPFSAAFLALVEMLRD
                     GSLLKAYLITLQPLALGIVLCAVAGIGFGIGMGLSRSVEWLSLPIFIILQAAPMAAII
                     PLITYIYGIGLTSKVLAVIMLAAPVIVMNSYKGIRNTPPSLLQMCRSFQGTRRQEVMK
                     IVLPHAAGLIFTGLRLGLAMGFIGIVIAELLITPTGIGDLITYNSSVADYPKMFAAIA
                     SIVALAASALYLLERLERRWFP"
     gene            4392..5141
                     /locus_tag="VFR57_02235"
     CDS             4392..5141
                     /locus_tag="VFR57_02235"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258222.1"
                     /translation="MIVEVKGVRKTYDNGVEALRDVDLSFPKGQLSTLLGPSGCGKTT
                     LLKIIAGLIPKNGGEVLVNGKAVHGPGPERAFVFQDFALLPWADVLRNVGFGLELRGV
                     ERNKRDEVARKYIAEVGLKGFEHRYPAQLSGGMRQRVGLARALTVDADIILMDEPFSS
                     VDEQTRRKFQEDLLDLLRHHQKTVIFVTHSIEEAAYLSDQIALLSPRPGRVSKIVRPA
                     VDRSKSPDEIRRDRGYLDTVDEIWGILRQYV"
     gene            5144..5869
                     /locus_tag="VFR57_02240"
     CDS             5144..5869
                     /locus_tag="VFR57_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0258223.1"
                     /translation="MLLSLVVWFVLWEIVGQMKFSSIVPPFSAVIEAGIEILPTEKFA
                     KAAIISLRSFGLGMALALAIGIPLGVAMARVKGVGRLLGMWVNIFVSAPISALVPILM
                     AVIGIGETTVVVTVFLFAVFVITLDTQVGITHADRSLVEMARSFGATRRQLYSKVLLL
                     SALPEILAGVRLGAIRGVKGVVIGQLLVAIIGVGELFETYSNNFLMEEFWALVLIVFL
                     FAFAVSEAIAYLERRVEYYAAAR"
     gene            complement(5936..7027)
                     /locus_tag="VFR57_02245"
     CDS             complement(5936..7027)
                     /locus_tag="VFR57_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEU0258224.1"
                     /translation="MKPFRLQVPDSALADLRERLARTRLPDEPPGPPWKTGASLAYVK
                     QLIEYWRSGFDWRAQEARINAFPQFKVPIDGVEVHFIHQRSASPDAMPLLLVHGWPGS
                     IVEFLGLIPRLAERFDVVAPSLPGYTLSFAPGQKRFGVEDIASTFTKLMTEVLGYKRF
                     GTQGGDWGAFITSRMAATRPELFTGLHLNYLTVPRDTTAADADWQAQLADWVKERSAY
                     SIIQGTRPQTLAYSLTDSPAGLAAWIAEKFHAWTDNDGTIESAIARDDMLANISLYWF
                     TGAIGSSFWTYYARTHGPWPVPFPITVPTAYAAFPKEILRPPRAVAEKYYTNIQRWTA
                     MEKGGHFAALEQPEALARDIGDFFASYQT"
     gene            complement(7024..7974)
                     /locus_tag="VFR57_02250"
     CDS             complement(7024..7974)
                     /locus_tag="VFR57_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258225.1"
                     /translation="MAKIFLALILLAATPLAAQEYPNRPIRLITPAAQGGTTDLLARV
                     FGQKLAESWGQQVLVDNRASAAGVIAGQLTTQAAPDGYTLLLAYHQHTVNAALNPKLP
                     YHPVNDFTPITQLTSAGLMLVVNPSTPVTNLAEFMQWTKNFQGALNFGSAGIGSGGHL
                     AGELFKLMAGVKAEHIPYKGAGPAMADLIAGQYHFNFSGLQGSQVQVRAGRLRAIAVT
                     TPKRLPSNPELPAMAEVLPGFEVVGWYGVIGPANMPQPLVQKLHGELVKILNQPDVKS
                     RIEADGSEPVGSSPEEFRRFMHADLNKWAKLVKESGAKLE"
     gene            complement(7999..8430)
                     /locus_tag="VFR57_02255"
     CDS             complement(7999..8430)
                     /locus_tag="VFR57_02255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0258226.1"
                     /translation="MAIEWKYSTDVVDWEEVSAMYRAAPLGDKKPSSLQTVFTNSMFK
                     CFVYERGHLVGAGRALADGADCSYICDIAVLPSHQGTGLAKQIMGYLVERSRGHRKIL
                     LYAVPGREPFYRKFGFMRMTTAMAIFENQEEQLERGYLSET"
     gene            complement(8435..8908)
                     /locus_tag="VFR57_02260"
     CDS             complement(8435..8908)
                     /locus_tag="VFR57_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEU0258227.1"
                     /translation="MVHNGLKILIAVALSACAAMQPRDPVQVTVAGMESLPAEGLEVR
                     MLVKLRVQNPNDVPIEYNGVFLKLDLLDQSFATGVSDERGSVPRFGESVISVPVTVSM
                     LRIATQGLGLLGGGSREKVTYSVEGKLGGSGLTSTRFQAKGELTMPGTSASPSKP"
     gene            complement(8962..10011)
                     /locus_tag="VFR57_02265"
     CDS             complement(8962..10011)
                     /locus_tag="VFR57_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0258228.1"
                     /translation="MFLMNCWYVAAWDHELIDGKLLARTILEKPVLIYRGNSGRVVAL
                     DNRCCHRGAWLSNGRREGDDIRCMYHGLKFDPTGKCVQIPGQENIPPKLGVRSYPAVE
                     RQHLIWIWMGEAARADPALIPSFTYLSDPGWRGKPDYMHYKANYLLIVDNLSDFAHLA
                     FVHTRTLGGSEEYAYKSKPTAIERLPDGFRVERWHMNADSPPFHKKVVRDAGKVDRRN
                     IARMYVPGIFFMETLFSPAGAGAEKGNLQDAKQYRNCQFFTPETKRSTHFFWDYLHDY
                     ELQDPTIALSLNNSMIEGFLEDKFIIEGQQEMLDADADFKMNAIVADAPLAHFRRTLA
                     KLIDAERGQSQPAAA"
     gene            complement(10040..11320)
                     /locus_tag="VFR57_02270"
     CDS             complement(10040..11320)
                     /locus_tag="VFR57_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEU0258229.1"
                     /translation="MRRAFWLSCAALLLGACAETARLPDSATVGPDPALPEPTRSIVP
                     TVKIAPAKGWPEGVTPKAAAGTQVSAFARDLQHPRWVYVLPNGDVLVAETNAPHRPEE
                     GKGIKGRAMTHFMKKAGAAVPSANRITLLRDADGDGLAELREVFLQGLNSPFGMALVG
                     SDLYVANTDAIVRFPYVQGQTRITAAGIKITELPAGPRNHHWTKNLIASLDGSKLYVT
                     TGSNSNVAEHGMQEEEGRAAIWEVDAKSGAYRIFASGLRNPNGLAWEPKTGALWTVVN
                     ERDELGSDLVPDYLTSVRDGAFYGWPYSYFGQHVDKRVQPPRPDLVAKAIKPDYALGN
                     HVAPLGLAMSGEGAFVGQHGSWNRRPPSGYNVVFIRFADGKPTGAPHEILGGFLSPEG
                     NAYGRPVGVAIDERGGLLVADDVGNTVWRVTAAR"
     gene            complement(11348..11683)
                     /locus_tag="VFR57_02275"
     CDS             complement(11348..11683)
                     /locus_tag="VFR57_02275"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU0258230.1"
                     /translation="MRLAIALLLCAGAAFAQEDQRGARFSLGAGVFGGESGGTGLPGA
                     GVNLVRNQHWRAGARPGAGAPKRRAERRDWPAIVRASVARLEGDAAASPITRDRTQYT
                     AGILAAYEF"
     gene            complement(11680..>12824)
                     /locus_tag="VFR57_02280"
     CDS             complement(11680..>12824)
                     /locus_tag="VFR57_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0258231.1"
                     /translation="QYGCEFLSFTQDASGVRTETSRGPITSKFLVGCDGGSSAVRQQL
                     GIALNGEGDLLRLRQGFFYCEELFDRIPIGNGPGHGRHYHVADERASFLIMQDSTRHW
                     TLHAVLDDDAAMAPQFEKTIGVPVKYEMLYVGEWKQNLLLADRFGKGRVFLAGDAVHL
                     VIPTGGLGMNGGVGDAMDLGWKLAATLQGWGGPRLLESYEVERRQVGERNVAASRYAS
                     LGRRKWRSLWRNLEELAAVAEVEQRKSNEMVGAELGYRYVGSPIIADEPDGPEHDFRR
                     YIPSTWPGARLPHLWLEGHVAAQDLIGTGYTLLRLDGSREATALEQSFRALRAPLQVL
                     DIGDRKARALYGHDLLLLRPDLHVAWRGNRPPEDPRALAKLVTGNR"
BASE COUNT         2140 a         4173 c         4175 g         2336 t
ORIGIN      
        1 gtgtcggcga agacctcgcc gcccgcgatg tgatcggcgt ggtcgtggct gtagacgaca
       61 taccggaccg gctggttgaa ccgctgggtg atctccgcct cgagccagcg cgccgcgtcc
      121 gcgttgatgg ggtccgtcgc gatcacgccg gccggcgtca cggcgaagac ggaatagtgg
      181 ttggcgtggc ggaagcgata gacctccccg gcgatcttcg tgatctcccg gaccggcgcc
      241 tgctgcgatt gcgcccacgg cgcgcccgcc agcagcgccg cgatcccgag cactcccagc
      301 gacacggccc gtcgtctccg catagggcgt tgtccttcgg gtggtgacat ggcctactgc
      361 cagcccttct ccttgtagta cctggccgcg ccctcgtgca gcggcgccga caggccgttc
      421 ttgaccatgt ccttcgtctc caggtaggcg aaggccggat gcagcttcct gaactcgtcg
      481 aagttgtcga agaccgcctt cacgacctgg tacacgacgc tggccggcat cgtcgtggac
      541 gtgaccagcg tcgccagcac gccgtacgtc ttcgtgggct ccgggttgtt cctgtagatg
      601 ccccccggta tcgtcgcgta ggcgtagaag ggattgccct tcacgagctt gtccacggcc
      661 gggcccctga ccggcacaag cttggcgccg caggcggtgg tcggctcctg gatgttggcc
      721 gacggatggc cgacgccata gaagaagccg tcgatcttgc cgtcgcacaa tgccgggccg
      781 tgctcgtcag ccttcaattc cgcggcgagc gagaagtcgg acatcttcca gcccatcgcg
      841 ccgagcagct cctccatcga ggcgcgcgtg cccgagcccg ggttgccgac gttgaagcgc
      901 ttgcccttga agtcggcgaa gctcttcacg ttcgcctcct tccgcgccag caccgtgaaa
      961 ggctcggggt gcagcgacat caccgcgcgc agctccttga acgggccgtc cttgtactgc
     1021 gcttcgccct tggcggcgtt gtactgcacg tcggactgcg ccacgcccag gtcgagctcg
     1081 cccgccttga tggtgttgat gttgaacacc gagccgccgg tggattccac cgagcagcgg
     1141 atgccgtgct tcgcacggtc cttgttcaca aggcggcaga tcgcgccgcc ggccgcgtag
     1201 tacacgcccg tcacgccgcc ggtgccgatg gtcatgaact tctgctgcgc ctgcgcgctc
     1261 ggcgcgccgg cgaacgccgc ggcgacggcc gcgaacgcaa caacccgttt catatggtct
     1321 gctcctctaa ggtagatatg gctgtgccta gtttatcgac tagctcgtcc agttgggatt
     1381 cctcgacaat gaacggcggg gcgagcagaa cgtggtcgcc ctgcacgcca tcgatggtgc
     1441 cgcccatcgg gtagcacatg aggccagcct ccagcgcctt gcgcttgagc cgggcgtgca
     1501 tgcgcagctt cggatcgaaa ggtttcttcg cggcgcggtc ttcgacgatt tcgatgcccc
     1561 agaacaggcc gcgcccgcgg atgtcgccta cgtgcgggtg cccggcgaac gcttccctca
     1621 accggcgctc gagcgccgtt ccgaggggaa ataccttttt catcacgcca tcttcgtgca
     1681 gacgcttctg cacggcgagc gctccggcgc aggccgcggc gtggccgatg tacgtgtgcc
     1741 cgtgctggaa gaaaccggtg ccgcgctgga tcgtgtcggc gatctccttc gagaccagga
     1801 gggcgccgat cggctggtag cccgcgccga gtcctttggc gatcgcgacg aggtccggaa
     1861 cgatcccttc ctgctcgaag gtccagagcg tgccgcagcg ccccatgccg cacatcacct
     1921 cgtcgaggat cagcagcacg ccgtagcggt cgcacaggtc gcgcacgcgc ttgaaataac
     1981 ccggcaccgg aggtacagcg ccgatcgtcg cgcccgccac cgtctcggcg acgaacgcga
     2041 tcacgctctt ctgctggaat tgcgcttcga gctcctcgct aaggcgctcg acataggcgg
     2101 ggtcgctctc cccgggattc ttgccgcgat aggcatagca gggcgatacg tgcgtcgtcc
     2161 tcaccagcaa aggctcgaac tgcttgcgcc gccaggcgtt gccgcccacc gcgagcgcgc
     2221 cgagcgtgtt gccgtgatac gactgacgcc ggccgatcac atgcgcgcgc tgcggctcgc
     2281 ctttctccac gaaatactgg cgcgcgagct tgagcgcggc ctcgatcgct tccgagccgc
     2341 ccgaaacgaa gtaaaccttg tcgaacgact tcggcgcgcg cgcgatgagc gcgtcggcaa
     2401 gctcttccat cggctcgttg gtgaagaacg aggtgtgcgc gaagggcaac ttctcgagct
     2461 ggcgccggat cgcctggatc accgcggcat cggagtgacc gaggcaagag accgcggcgc
     2521 cgcccgaagc atcgagatag cgcttaccgt cccggtcgat gagataagca ccctcgccgc
     2581 gcaccgccac tcgatacgtc aggcgcggat cgcggtggaa gacgtgcgtc acggcgtcaa
     2641 acatagcatg tatggcagta agattgcctc cgcacgagtt tctaacgccc atttccccag
     2701 gaggagtgat gttcagaatc cttgcagtcg cggcggccgc agcgctgttg agcggcgcag
     2761 caacggcgca gaacaagatc aagtacgcgc tcggcgacgt catcgggatc gacgacctgc
     2821 cgctgctcgt cgccgcggag cgctcgaaaa agcgcggcgt cgaggtcgag atcacgccgt
     2881 tcaagaacga ggacattgcc acgcaggcgg tgatcaacgg ccaggccgac atcggccagg
     2941 gcacgccgta cgcggcgatc gagaaggtga ccgtgccgat ccgcttcttc gtgcagcttt
     3001 ggtcgctgca gtttttcccg gtggtggcga acgagcacta caagagctgg aaggacctcg
     3061 acggccagga gatcgccgtg cagggccgcg gctcgggcac cgaggcgatc atgctgctca
     3121 tggcgaagca gcatggcatc aagtacaaga acgtgagcta cgttcccggc tcgcaggtgc
     3181 gtgcgctcgc gctgatcaaa ggcaacatcc gcgcctcgat cctcgacgcg acgaacaaga
     3241 acttcctgat gcgcgaggcg cccggcaagt tcaccatcct gccgctcggt gagatcaagg
     3301 cgagcgacga gtcgctgttc gcgcggctgg agtacctgca gaagaaccag gctgcggtgc
     3361 aggtgttcgt cgaggagctg gtgcgcgtca atcgcgagat caacaagaac ccgatgtcga
     3421 tcgtcgaaga gcgcaagaaa ctcggcttgt tgaaagacct ccccgcgaag ctcgaggagg
     3481 agctgccaac gtacttcaag gaaggcgtcg ccggcggcat cttcccgaac gacggcggcg
     3541 ccgagaacgc ggccaagacc gacctggagt tcttcgcgct ctcgggctcg atcaagaaag
     3601 agaacccgaa ggtcgacgac tactggcacc tcgccccgct caaggcggct ctgtccaggc
     3661 tgaagtagcg ctttgcgggg ctggcagcta gcgtcgttcg cgctcttctt cggattgtgg
     3721 gagctggccg gccgctggcc ggtcagcgcc gcctttccgc cgttcagcgc ggctttcctc
     3781 gcgctcgtgg agatgctgcg cgacggcagc ctgctcaagg cctacctgat cacgctgcaa
     3841 ccgctcgcgc tcggcatcgt gctctgcgcc gtggcgggca tcggcttcgg catcggcatg
     3901 gggctttcgc gctcggtgga gtggctgtcg ctgccgatct tcatcatcct gcaggccgcg
     3961 ccgatggcgg cgatcatccc gctcatcacc tacatctacg gcatcggcct aacttcgaag
     4021 gtgcttgcgg tgatcatgct cgcggcgccg gtgatcgtga tgaattcgta caagggcatc
     4081 cgcaacacgc cgccttcgct actgcagatg tgccgctctt tccaaggcac gcggcgccag
     4141 gaagtcatga agatcgtgct gccccatgcg gcgggcctca ttttcaccgg cctgcggctc
     4201 ggcctcgcca tgggcttcat cggcatcgtc atcgccgagc tcctgatcac gcccaccggc
     4261 atcggcgacc tcatcaccta caacagctcg gtcgccgact atccgaaaat gttcgcggcc
     4321 atcgcgtcga tcgtcgcgct cgcggcgagc gcgctctacc ttctcgagcg cctcgagcgc
     4381 agatggtttc catgatcgtc gaagtcaaag gcgtgcgcaa aacctacgac aacggcgtgg
     4441 aagcgctgcg cgacgtcgac ctgtcgttcc cgaagggcca gctctccacg ctgctcggcc
     4501 cgagcggctg cggcaagacc acgctcctca agatcatcgc gggcctgatt ccgaagaacg
     4561 gcggcgaagt cctggtgaac ggcaaggcgg tgcacggccc gggacccgag cgggcgttcg
     4621 tctttcagga tttcgcgctg ctgccgtggg cagacgtgct gaggaacgtg ggcttcggcc
     4681 tggagctacg cggagtagaa agaaacaaac gagacgaagt tgcgcgaaag tacatcgccg
     4741 aggtgggctt gaagggtttc gagcaccgct accctgcgca gctctccggc ggcatgcgcc
     4801 agcgcgtggg gctcgcgcgc gcgctcaccg tcgacgcgga catcatcctc atggacgagc
     4861 cgttctcctc ggtggacgag cagacgcggc gcaagttcca ggaggatctc ctcgacctcc
     4921 tgcgccacca tcagaaaacg gtgatcttcg tgacgcactc gatcgaggag gccgcgtatc
     4981 tctccgacca gatcgcgcta ctctcgccgc gtccagggcg cgtgtcgaag attgtgcgcc
     5041 cggcagtcga tcgctcgaag agtccggacg agatccgccg cgaccgcggc tacctcgaca
     5101 ccgtcgacga gatctggggg atcctccggc aatacgtatg aggatgcttc tttcccttgt
     5161 cgtctggttc gtgctctggg agatcgtcgg ccagatgaag ttctcgagca tcgtgccgcc
     5221 gttcagcgcg gtcatcgagg ccggtatcga gatcctgccc accgagaaat tcgccaaagc
     5281 cgcgatcatc tcgctgcgct ctttcggcct cggcatggcg ctcgcgctcg cgatcggcat
     5341 tccgctcggc gtcgccatgg cgcgcgtcaa gggcgtcggc cggctgctcg gcatgtgggt
     5401 caacattttc gtgagcgcgc cgatctcggc gctggtgccg atccttatgg cggtgatcgg
     5461 cattggtgag acgactgtcg tcgtcacggt gttcctcttc gccgtcttcg tcattacgct
     5521 cgacacccag gtcggcatca cgcacgccga ccgctcgctt gtcgagatgg cgcgctcctt
     5581 cggcgcaacg cggcgccagc tctactcgaa ggtcctgctt ctgagcgcgc tcccggagat
     5641 cctcgccggt gtgcgcctcg gcgcaatccg cggcgtgaaa ggcgtggtca tcggccagct
     5701 cctcgtcgcc atcatcggcg tgggcgagct cttcgagacc tactccaaca acttcctcat
     5761 ggaagagttc tgggcgctcg tcctcatcgt attcctcttc gccttcgccg tgtccgaggc
     5821 gatcgcctac ctcgagcgcc gcgtcgagta ctacgccgcc gcccgctaat accaacccga
     5881 attcggggtc agggtcttaa ttctgttcgc gcacaattcg caccctgacc ccgaattagg
     5941 tttggtatga ggcgaagaag tcgcctatat cccgggcaag ggcttcgggc tgttccaagg
     6001 cagcgaagtg gccgcccttc tccatcgcgg tccagcgctg gatgttggtg taatacttct
     6061 ccgccaccgc gcgcggcggg cgcaggattt ccttcgggaa agcggcatag gcggtcggta
     6121 cggtgatcgg gaacggcacg ggccatggac cgtgagtgcg cgcgtagtag gtccagaacg
     6181 aggagccgat ggcaccggtg aaccagtaga gcgagatgtt ggcgagcatg tcgtcgcgcg
     6241 cgatcgcgct ctcgatcgtg ccgtcgttgt cggtccaggc gtgaaacttc tcggcgatcc
     6301 acgccgcgag cccggctggc gagtcggtga ggctgtaggc gagcgtctgc gggcgcgtgc
     6361 cttggatgat cgagtacgcg gagcgctcct ttacccagtc ggcgagctgc gcctgccagt
     6421 ccgcgtcggc ggcggtggtg tcgcgcggca ccgtcaggta attgaggtgc aggccggtaa
     6481 agagctcggg acgcgtagcg gccatgcgcg aggtgatgaa cgcgccccag tcgccgcctt
     6541 gggtgccgaa gcgcttgtaa ccgagcacct cggtcatcag cttcgtgaag gtgctggcga
     6601 tgtcttcgac gccgaaacgc ttttgtcccg gcgcaaagga aagcgtgtag cccggcagcg
     6661 agggcgcgac gacgtcgaag cgctccgcga ggcgcgggat caggccgagg aactcgacga
     6721 tcgagcccgg ccagccgtgt accagaagca atggcatggc atccgggctc gccgagcgct
     6781 gatggatgaa gtgcacctcg acgccgtcga tgggcacctt gaactgcggg aaggcgttga
     6841 tgcgcgcttc ctgtgcgcgc cagtcgaagc cgctcctcca gtactcgatg agctgcttca
     6901 cataagcaag gctagcgccg gtcttccaag gcggacccgg cggctcgtca ggcagccggg
     6961 tgcgcgcgag gcgctcgcgc aggtccgcca gcgccgaatc cgggacctgc agccggaacg
     7021 gcttcattcc agcttcgcgc cgctctcctt gacgagcttc gcccacttgt tgagatcggc
     7081 gtgcatgaag cgccggaact cctcgggcga gctgcccacg ggctccgacc cgtcggcttc
     7141 gatgcggctc ttcacgtcgg gctgattgag gatcttcaca agctcgccgt gcagcttctg
     7201 cacgagcggc tgcggcatgt tcgccggtcc gattacaccg taccagccga ccacttcgaa
     7261 tccaggcagc acctctgcca ttgccggtaa ttcaggattc gacggaagcc gcttcggggt
     7321 ggtaaccgct atggcgcgta aacgcccggc gcgcacctgg acctgcgagc cctgcaggcc
     7381 ggagaagttg aagtggtact ggccggcaat gaggtcggcc atcgcgggtc cggcgccctt
     7441 gtacgggatg tgctcggcct tgacgccggc catgagcttg aagagctcgc cggcgagatg
     7501 gccgccgctg ccgatgccgg ccgagccgaa attgagcgcg ccctgaaagt tcttggtcca
     7561 ctgcatgaac tcggcgaggt tggtcaccgg cgtggatgga ttgaccacga gcatgagtcc
     7621 cgccgaggtg agctgcgtga tcggcgtgaa gtcgttcacc gggtgatacg gcagcttcgg
     7681 attcagcgcc gcgttcacgg tgtgctggtg gtacgcgaga agaagcgtgt agccgtccgg
     7741 cgcggcctgc gtggtgagct gcccggcgat cacgcccgcc gcgctcgcgc gattgtcgac
     7801 cagcacctgc tggccccacg actcggcgag cttctggccg aagacgcgcg caaggaggtc
     7861 ggtcgtgccg ccctgcgctg cgggtgtgat gaggcggatc gggcggttgg ggtactcctg
     7921 cgccgcgagc ggcgtggctg caaggaggat tagagcaagg aatatttttg ccatgacgtt
     7981 atccgatagg ctgaactgct acgtctcact cagatacccg cgctcgagct gctcctcttg
     8041 gttctcgaag atggccattg cggtagtcat gcgcatgaag ccgaactttc gatagaaagg
     8101 ctctctgcca ggtaccgcgt acaggaggat cttccggtgc ccgcgcgagc gctccaccag
     8161 gtaccccatg atttgcttgg ccagtccggt cccctggtga ctcggcagga ccgcgatgtc
     8221 gcagatatag gagcaatcgg ctccgtcggc cagcgcgcgg cctgcgccaa cgaggtggcc
     8281 ccgctcgtag acgaagcact tgaacatgct gttcgtgaag accgtctgta ggctcgaggg
     8341 cttcttgtct ccgagaggcg ccgcccggta catggccgag acctcttccc agtcgactac
     8401 atcagtagag tacttccatt cgatcgccac tttgctacgg cttcgatggg gaagcgctcg
     8461 tacccggcat cgtcaactcg cctttcgcct gaaatctggt cgaggtaagg ccggagccgc
     8521 cgagcttgcc ttccacggag taggtcacct tctcgcggga gccgccgccg agcaggccga
     8581 gaccctgggt tgcgatgcgc agcatcgaaa ccgtgaccgg cacgctgatg accgattcgc
     8641 caaagcgcgg aacgctgccg cgctcgtcgc tcaccccggt cgcgaacgac tggtccagca
     8701 agtccagctt caaaaacacg ccgttgtact cgatcggcac gtcgttggga ttctgcaccc
     8761 tcagcttgac cagcatccgc acttccaggc cttcggccgg taaggattcc atgcctgcca
     8821 cagtgacctg aaccgggtca cgtggttgca tggcggcgca cgcgcttagc gcgaccgcga
     8881 tgagaatctt caggccgtta tgcaccatgg tcggatctta accccgctcg aaaatcaatt
     8941 gtcaccctga ccccgaattc gtcaggcggc cgcgggctgc gactgccccc gctccgcgtc
     9001 gatcagcttc gccagcgtgc ggcggaagtg ggcgagcggc gcgtcggcga cgatcgcatt
     9061 catcttgaag tctgcatcgg cgtcgagcat ctcctgctgg ccttcgatga tgaacttgtc
     9121 ctcgaggaac ccctcgatca tgctgttgtt caacgagagc gcgatggtcg gatcctgcag
     9181 ctcgtagtcg tgcaggtagt cccagaagaa atgcgtcgag cgtttcgtct ctggcgtgaa
     9241 gaactggcag ttgcgatact gcttcgcgtc ctgcaggttg cctttctcgg cgcccgcgcc
     9301 ggccggcgaa aacagtgtct ccatgaagaa gatgccgggc acgtacatgc gcgcgatgtt
     9361 gcggcggtcg actttgcccg cgtcgcgcac caccttcttg tggaacggcg gcgaatcagc
     9421 gttcatgtgc cagcgctcga cgcggaagcc gtcgggcagg cgctcgatgg cggtgggctt
     9481 ggacttgtac gcgtactcct ccgaaccgcc gagcgtgcgc gtatgcacga aagcgagatg
     9541 cgcgaagtcg gagaggttgt cgacgatgag cagatagttc gctttgtagt gcatgtagtc
     9601 cggcttgccg cgccagccgg gatcgctcaa ataggtgaag cttggaatga gcgcgggatc
     9661 ggccctcgcc gcctcgccca tccagatcca gatgagatgc tggcgctcca ccgccgggta
     9721 gctgcgcacg ccgagcttcg gcggaatgtt ttcctggccc gggatctgga cgcactttcc
     9781 cgtcgggtcg aacttgaggc cgtggtacat gcagcggatg tcatcgccct cgcgccggcc
     9841 gttcgagagc cacgcgccgc gatgacagca gcggttgtcg agcgcgacca cgcgtccgct
     9901 gttgccgcga tagatgagca ccggcttctc caggatcgtg cgcgcgagca gcttgccgtc
     9961 gatgagctcg tggtcccacg ccgcgacgta ccagcaattc atcaggaaca ttgcagcctc
    10021 ctcaggctgc aagtttacgt cagcgtgccg cggttacgcg ccagaccgtg ttgccgacat
    10081 cgtcggcgac gaggagaccg ccgcgctcgt cgatcgctac gcctacaggg cgaccgtacg
    10141 cgtttccttc ggggctaaga aagccaccaa gaatttcgtg cggcgcgccg gtcggtttgc
    10201 cgtccgcgaa gcggatgaac acgacgttgt agccgctcgg cggacgccgg ttccaggagc
    10261 cgtgctgccc gacgaatgcg ccctcgccac tcattgccag tccgagcgga gcgacatgat
    10321 tgccgagcgc gtagtcgggt ttgatcgcct tggcgacgag atcggggcgt ggcggctgca
    10381 cgcgcttgtc gacgtgctga ccgaagtagc tgtacggcca gccgtagaag gcgccgtcgc
    10441 gcacggaagt gaggtaatcg ggaacgagat cgctgccgag ctcgtcgcgt tcgttcacca
    10501 ccgtccacag ggcgccggtc ttcggctccc aggcgagccc gttgggattg cgcaggccgg
    10561 aagcgaaaat gcggtacgcg ccgctcttcg catccacttc ccagatggcg gcccgcccct
    10621 cctcctcctg catgccgtgc tcggcgacgt tgctgttgga gccggtggtg acataaagct
    10681 tgctgccatc cagactcgcg atcaggttct tcgtccagtg atggttgcgc ggccccgccg
    10741 gcagctcggt gatcttgatc ccggccgccg tgatgcgcgt ctgcccctgc acgtagggga
    10801 agcgtacgat cgcgtcggtg tttgcgacgt acaggtcgct gccgaccaac gccatgccga
    10861 agggcgagtt caggccctga aggaacacct ctcgcagctc cgccaagccg tcgccgtcgg
    10921 cgtcgcgtag cagcgtaatg cggttcgcgc tcggcaccgc tgcgcccgcc ttcttcatga
    10981 aatgcgtcat ggcgcggccc ttgatgcctt ttccctcttc cggccgatgg ggcgcattgg
    11041 tttcggccac cagcacgtcg ccgttaggca gcacatagac ccagcgcgga tgctgcaggt
    11101 cccttgcgaa cgcgctcacc tgcgtgccgg ccgccgcctt cggcgtcacg ccttcgggcc
    11161 agcccttggc cggcgcgatt ttcaccgtag ggacgatgct ccgtgtcggc tcgggcagcg
    11221 cgggatcggg cccgaccgta gccgaatccg gaaggcgcgc ggtttccgcg caagcgccga
    11281 gaagcagggc cgcgcatgag agccagaagg cgcgtctcat gtgcgcgctg tatgcaagta
    11341 ttgcgcccta aaactcgtag gcggcgagga tgccagcggt gtactgcgtt cggtccctgg
    11401 tgatcgggct tgcggcggca tcgccttcca ggcgcgcaac gctcgcgcgc acgatcgcgg
    11461 gccagtcgcg gcgttctgct cgacgctttg gtgcgccggc gcctggacga gcgcccgcgc
    11521 gccagtgctg gttgcgcacg aggttgacgc cggcgccggg caagcctgtg ccgccggact
    11581 cgccgccgaa aacgccggcg ccgagactga aacgcgcgcc gcgctggtcc tcctgcgcaa
    11641 acgccgcgcc ggcgcatagc aggagcgcga tggcgagtct catctgttgc cggtcacgag
    11701 cttggcgagc gcgcgcgggt cttcgggcgg ccggttgccg cgccatgcga cgtgcaggtc
    11761 cgggcgcagc aggaggaggt catggccgta aagtgcgcgt gctttgcgat cgccgatatc
    11821 gagcacctgc agcggcgctc gcaaggcgcg gaacgattgt tccagcgccg tagcttcgcg
    11881 ggaaccgtcc aggcgaagaa gcgtatagcc cgttccgatc aggtcctgcg cggcgacgtg
    11941 gccctcgagc cacaagtgcg gaaggcgcgc accgggccag gtgcttggga tatagcgccg
    12001 gaagtcgtgc tcggggccgt cgggctcgtc agcgatgatg ggcgagccca catagcgata
    12061 gccgagttcg gcgccgacca tctcgttcga ttttcgctgc tcgacctcag cgaccgcagc
    12121 gagctcttcg aggttgcgcc aaagcgatcg ccacttgcgg cggccaagcg acgcataccg
    12181 cgaggcggcg acattacgct cgccgacctg gcggcgctca acctcgtaag actcaagcaa
    12241 ccttggaccg ccccaccctt gcagcgtcgc ggcgagcttc cagccaagat ccatcgcgtc
    12301 gcccacgccg ccgttcatgc caagcccgcc ggtcgggatc acgagatgca cggcgtcgcc
    12361 ggcgagaaac acgcggccct tgccaaaacg gtcggcgagg agcaggttct gcttccactc
    12421 gcccacgtag agcatctcgt acttcaccgg cacgccgatc gttttctcga actgcggcgc
    12481 catcgccgcg tcgtcgtcga gcaccgcgtg cagcgtccag tgccgggtcg agtcctgcat
    12541 gatgaggaaa ctggcgcgct catcggcgac gtgatagtgg cggccgtgcc cggggccgtt
    12601 gccgatcggg atgcggtcga acaattcctc gcagtagaag aatccctgcc gaagacgcag
    12661 aagatcgcct tcgccgttca acgcgatacc gagttgctgg cgtacggcgc tcgagccgcc
    12721 gtcgcaaccg acgaggaact tcgatgtaat cgggccgcgc gaggtctcgg ttctgactcc
    12781 gctcgcgtcc tgtgtgaacg aaaggaactc gcaaccgtat tgca
//
LOCUS       DASTPD010000057         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1456615, whole genome shotgun sequence.
ACCESSION   DASTPD010000057 DASTPD010000000
VERSION     DASTPD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456615"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="gap"
                     /locus_tag="VFR57_02285"
     CDS             complement(<1..866)
                     /gene="gap"
                     /locus_tag="VFR57_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0258232.1"
                     /translation="MTIKAAINGFGRIGRMVLRAHYESGKKHGIQFVAINDLGDAQTN
                     AHLLKYDTAHGPFPANVRVEGDSIVVNNDRIKVVAERDPAKLPWRTLGVDVVMECTGL
                     FTSKAKAGGHIQAGARKVIISAPGEKDVDRTVVFGVNHRSLKESDSVISNASCTTNCL
                     APLVKAIDERIGVACGLMNTIHSFTNDQVLTDVFHKDLRRARAAGHNMIPTKTGAAAA
                     VGLVLPHLNGKLDGYSIRVPTNNVSIVDLTFVAKKPTTVEEVNRAVKQACDGDLKGVL
                     EYSTEPLVSSDF"
     gene            complement(863..2878)
                     /gene="tkt"
                     /locus_tag="VFR57_02290"
     CDS             complement(863..2878)
                     /gene="tkt"
                     /locus_tag="VFR57_02290"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0258233.1"
                     /translation="MDSSVKAKASEAELANSIRALAMDAVEVAKSGHPGMPMGMAEIA
                     VALWKRHLRHNPSNPAWFNRDRFVLSNGHGSMLLYSLLHLTGYALPLEELKRFRQLGS
                     RTPGHPERGIAPGVETTTGPLGQGLANAVGMALAEKLLAARFNKPGHSIIDHFTYVFL
                     GDGCMMEGISHEACSLAGTLGLGKLIAVYDDNGISIDSDKGEIRQWYTDDVRRRFDAY
                     GWHIVGPIDGHDVEAVDKALKRAKREKDRPTLLIAKTTIAKGAPKKANTGAAHGAPLG
                     AEEIAATRVAIGWPHAPFVVPEHVYAGWDAREAGKKAERRWNRFFKSYKVSFPEEAAE
                     LERRVAPQLPADFEPRVQALISEFDGRAETLATRKASQNVLDALVPALPELVGGSADL
                     TGSNLTWSKASTPVLNAAPAGNHIFYGVREFGMCAVMNGLALHGGFVPYGGTFLVFSD
                     YARNALRLAALMGIQVIYVFSHDSIGLGEDGPTHQPIEHAATLRLMPNMEVWRPCDTV
                     ETAFAWVAALKRRNSPTALLLTRQNVPFVKRQSRGDIAKGGYVLADAADAKAVIIATG
                     SEVSLALAAQKQLAEEGVPVRVVSMPSTTEFDRQSAEYRNSVLPAALPKVAVEAGVSD
                     YWRKYVGPAGGVVGIDRYGESAPAAEVFKHFGFTADNVAKVVRSILV"
     gene            2906..3778
                     /gene="nudC"
                     /locus_tag="VFR57_02295"
     CDS             2906..3778
                     /gene="nudC"
                     /locus_tag="VFR57_02295"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEU0258234.1"
                     /translation="MELPFRAPSEFEPLCFTEVTVEGHWFAFCGDKLLVELGPLEKPS
                     DDPRFRARPAWARLPKYRSDAQPERTLYLGRLRGVDCWAAEFGPGAAAPAGAAWEGLR
                     PLFSVLDDDHFALAGRALQLLEWDRTHQFCGRCGTRTEPKREERVRVCPACKLSAYPR
                     VAPAVMALVKREKQILLARSPHFPAGMYSALAGFVEPGESLEQCLAREVAEEVGVRIG
                     ATRYFASQSWPFPHSLMIAFVCEYASGELKPQEGEIEDAKWFDLLHLPKLPSKISIAR
                     KLIDAVCQEIEEQT"
     gene            3912..>5163
                     /gene="speE"
                     /locus_tag="VFR57_02300"
     CDS             3912..>5163
                     /gene="speE"
                     /locus_tag="VFR57_02300"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU0258235.1"
                     /translation="MNGLHLIGDLTGCRCDPQLLLDGERFRAKCLEMVAASGLTTMDA
                     TFHQFEGSGFTGTVVLAESHLAIHTWPERQGLTLDVYVCNYSADNSAKARRLFDLLVD
                     YFQPTEVARHEVDRGGHLLMEPLNDSTGFYIKAGSQLGEWQTRFQKMAIYETPHYGKI
                     FRLDGFNMTSEKEEFVYHENLIHPALTAHMAPKRVLIIGGGDGGSSEEALKHPSVEQV
                     TMVEIDGDVIEVAKTHFHAVHQGVFDNPKLRVLVDDGMKFVRETQEKFDLIALDLNDP
                     MGPAEALYSSEFFQQLRHTLAPGGALTLHIGSPVARPERVAQLAQRLNTVFRIVRTYT
                     MYIPLYGSLWAMAVCSDKLDPKSFTADEIDRRIEARKLADLRYYNGETHEGVFALPNF
                     VRDLVVPARLKQPARGRRLGVVRAA"
BASE COUNT          862 a         1665 c         1717 g          919 t
ORIGIN      
        1 ttgaagtcgg acgacaccag cggctcggtg ctgtactcga gcacgccctt gaggtcgccg
       61 tcgcacgcct gcttcacggc gcggttcact tcctcgaccg tggtgggctt cttcgccacg
      121 aaggtgaggt cgacgatcga gacgttgttg gtcggcacgc ggatcgagta gccgtcgagc
      181 ttgccattga gatgcggcag cacgagtccc acggcggccg ctgcgccggt cttggtcggg
      241 atcatgttgt gcccggcggc gcgcgcccgg cgcaggtcct tatggaatac gtcggtcagc
      301 acctggtcat tcgtgaacga gtggatcgtg ttcatcagcc cgcaagcgac gccgatgcgc
      361 tcgtcgatcg ccttgacgag cggcgcgagg cagttcgtcg tacacgaggc gttcgaaatg
      421 acgctgtcgc tttccttcag ggagcgatgg ttaacgccga aaaccaccgt gcggtccacg
      481 tccttttctc ccggcgcgga gatgatcacc ttccgggcgc ctgcctggat gtgtccgccg
      541 gccttcgcct tgctcgtgaa aaggccggtg cactccatga cgacgtcgac gccaagcgtg
      601 cgccatggca gcttggccgg gtcccgctcg gccacgacct tgatgcggtc gttgttgacc
      661 acgatgctgt cgccctcgac gcgtacgttc gccgggaacg gcccgtgcgc cgtgtcgtac
      721 ttcagaagat gcgcgttggt ctgcgcgtcg ccgaggtcgt tgatggcgac gaactggatg
      781 ccgtgcttct tcccgctttc ataatgggcg cgcagcacca tgcggccgat acgcccgaaa
      841 ccgttgatcg ctgccttgat ggtcatacga gtatgctcct gacgactttc gccacgttgt
      901 ccgctgtaaa accgaagtgc ttgaacacct ccgccgccgg cgccgattcg ccgtagcggt
      961 cgatgccgac caccccgccc gcgggtccga cgtacttgcg ccaataatcg ctgacgcccg
     1021 cctcgaccgc taccttaggg agtgccgccg gcagcacgga gttccggtac tcggcgctct
     1081 gccggtcgaa ttccgtggtc gacggcatgg agaccacccg gaccggaacc ccttcctcgg
     1141 cgagctgctt ctgcgcagcc aaggcgagcg acacttccga gccggtggcg atgatcacgg
     1201 ctttggcatc ggcggcgtcg gcgagaacgt agccgccctt cgcgatgtcg ccgcgggatt
     1261 ggcgcttcac aaacggcacg ttctggcgcg tcaaaaggag cgcggtgggc gagttcctgc
     1321 gcttgagcgc ggctacccag gcgaaggccg tctccaccgt gtcgcacggg cgccagacct
     1381 ccatattggg catcaggcgc aaggtcgccg cgtgctcgat cggctggtgc gtggggccgt
     1441 cctcgcctag gccgatggag tcgtgcgaga agacgtagat cacctgaatg cccatgagcg
     1501 cggcaaggcg aagcgcgttg cgcgcgtaat ccgagaaaac gaggaacgtg ccgccgtacg
     1561 gcacgaagcc accgtgcagc gcgaggccgt tcatgacggc acacatgccg aactcgcgta
     1621 cgccatagaa gatgtggttg cccgccggag cggcgttcag caccggcgtg gaagccttgg
     1681 accaggtgag attggagccg gtaaggtcgg cggagccgcc gacgagttcc ggtagcgccg
     1741 gaacgagagc gtccaggaca ttctgagacg ccttgcgcgt cgccagggtc tcggccctgc
     1801 cgtcgaactc ggaaatgagc gcctgtacgc gcggctcgaa atctgccgga agctgcgggg
     1861 ctacgcggcg ctcgagctcg gctgcctcct cggggaagct gaccttgtag ctcttgaaaa
     1921 agcggttcca gcggcgctcg gctttcttgc cagcctcgcg cgcgtcccag ccagcgtaga
     1981 catgctcggg cacgacgaac ggcgcatgcg gccagccgat ggccactctg gtggccgcaa
     2041 tctcctcggc cccgagcggc gcgccgtgtg ccgcgccggt gttggccttc ttgggcgcgc
     2101 ccttggcgat cgtggtcttg gcgatgagga gcgtcgggcg gtccttctcg cgcttggctc
     2161 gcttgagcgc cttatcgacc gcctccacgt cgtggccatc gatcgggccg acgatgtgcc
     2221 agccgtaggc gtcgaagcgc cggcgcacgt cgtcggtgta ccactggcgg atttcaccct
     2281 tgtcggaatc gatcgagatg ccgttgtcgt cgtagacggc aatgagcttg cccaggccga
     2341 gcgtgcctgc gagcgagcaa gcctcgtggg agatgccttc catcatgcag ccgtcgccga
     2401 ggaagacgta ggtgaagtga tcaatgatgc tgtgccctgg cttattgaaa cgcgcggcga
     2461 gaagcttttc ggcgagcgcc atgcccaccg cgttggcgag cccctgcccc agcggtccgg
     2521 tggtcgtctc tacgccaggc gcgatgccgc gctccgggtg gcccggggtc ctcgagccga
     2581 gctggcggaa gcgctttagc tcctcaagcg gcagcgcgta gcccgtgagg tgcagcaggc
     2641 tatagaggag catggatccg tggccgttcg agagcacgaa acggtcgcgg ttgaaccacg
     2701 ccgggttgga ggggttgtgg cgcagatggc gcttccagag cgccaccgcg atctccgcca
     2761 tgcccatcgg catgcccggg tgtcccgatt tcgccacctc cactgcgtcc atggccagcg
     2821 cgcgaatgga attcgcgagt tcggcctcgg aagccttggc ctttacggag gaatccatcg
     2881 ggaggcgcaa attatactca gcgcgatgga gcttcccttc cgcgcaccga gcgagttcga
     2941 gccgctctgc ttcaccgaag tgacagtcga aggacactgg ttcgccttct gcggcgacaa
     3001 gctgctcgtc gagcttggcc cgctcgagaa gccaagcgac gatccgcgct ttcgcgcgcg
     3061 gcccgcgtgg gcgcggctgc cgaagtaccg ctcggatgcg cagcccgagc gcacgctcta
     3121 tctgggccgg ctgcgcggcg tcgattgctg ggcggcggag ttcggtccgg gcgcagccgc
     3181 gccggcgggc gctgcgtggg agggcctgcg gccgctcttc tccgtgcttg acgacgacca
     3241 cttcgcgctc gccggccggg cgctgcagct cctcgagtgg gatcgcacgc accagttctg
     3301 cggacgctgc ggcacgcgca ccgagcccaa acgcgaggag cgcgtgcgcg tgtgcccggc
     3361 ctgcaaattg tcggcctatc cgcgcgtggc gcccgccgtc atggcgctcg taaagaggga
     3421 aaagcagatc ctgctcgcgc gcagtccgca cttccctgcg ggcatgtaca gcgcgctcgc
     3481 cggcttcgtc gagccgggcg agtcgctcga gcagtgcctc gcgcgcgaag tcgccgagga
     3541 agtcggcgtg aggatcggcg cgacgcgcta cttcgcgagc cagagctggc cgtttccgca
     3601 ctcgctcatg atcgcgttcg tctgcgagta cgccagcggc gaattgaagc cgcaagaagg
     3661 agaaatcgag gacgcaaagt ggttcgatct attgcatttg ccgaaacttc cgagcaaaat
     3721 ctcgatcgct cggaaattga ttgacgccgt ttgtcaagag attgaagagc aaacttagag
     3781 actcaggagg tcttgcacga gttgagcgcg attgaagagc gctgttttgc gccggaaggc
     3841 gcgaatggaa ggcgtggcct ggaacccaga tacgcggcag gctgcgcctt cgcattttgg
     3901 agggtacggg aatgaacggt ctgcatctca tcggtgacct caccggctgc cgctgcgacc
     3961 cgcagctcct tctggacggc gagcgcttcc gcgccaaatg cctcgagatg gtggccgcgt
     4021 ccggtcttac gaccatggac gcgacctttc accagttcga gggatccggc ttcacgggca
     4081 cggtcgtgct cgccgaatcg cacctcgcga tccatacctg gcccgagcgc caggggctca
     4141 cgctcgacgt gtacgtctgc aactacagcg ccgacaactc ggcaaaggcg cggaggctct
     4201 tcgacctcct ggtcgactac ttccagccga ccgaggtcgc acgccacgag gtggaccgcg
     4261 gcgggcacct gctcatggag ccgctcaacg actccaccgg cttctacatc aaggccggaa
     4321 gccagctcgg cgagtggcag acgcgcttcc agaagatggc gatctacgag acgccgcact
     4381 acgggaagat tttccgtctc gacggtttca acatgacctc cgagaaggag gagttcgtct
     4441 accacgagaa cctgatccac cccgcgctca ccgcgcacat ggcgccgaag agggtgctca
     4501 tcatcggcgg cggcgacggc ggctcctctg aggaggcgct caagcaccct tcggtcgagc
     4561 aggtgaccat ggtcgagatt gacggcgacg tcatcgaggt ggcgaagacg cattttcatg
     4621 ccgtgcacca gggcgtgttc gacaatccga agctgcgcgt gctggtcgac gatggcatga
     4681 agttcgtgcg cgagacacaa gagaaattcg acctgatcgc cttggatctc aacgatccga
     4741 tgggccccgc cgaagcgctg tactcctccg aattcttcca gcagctgcgc cacaccctgg
     4801 cgccgggagg agcgctgacg ctgcatatcg gatcgccggt ggcgcgcccc gagcgcgtgg
     4861 cgcagctcgc ccagcggctg aacaccgtgt tccgcatcgt gcggacttac acgatgtaca
     4921 tcccgctcta cggctcgcta tgggccatgg cggtgtgctc ggacaagctc gatccgaagt
     4981 cctttaccgc ggacgagatc gaccggcgca tcgaggcgcg caagctcgcc gacctgcgct
     5041 actacaacgg cgagacccac gaaggcgtgt tcgcgctgcc gaacttcgtc cgggacctgg
     5101 tggtcccggc gcgcctgaag cagccggccc gcggccgccg gctgggtgtg gtgagagcgg
     5161 ccg
//
LOCUS       DASTPD010000058         4263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1048007, whole genome shotgun sequence.
ACCESSION   DASTPD010000058 DASTPD010000000
VERSION     DASTPD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048007"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFR57_02305"
     CDS             complement(<1..296)
                     /locus_tag="VFR57_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258236.1"
                     /translation="MARTIIVDAEEKVFCPKCSHRFPLSEGISRQAIERHAGELERSL
                     AEHKKKLEAQLAAEAKARFEIQLKALNEALSAKEGTLAKFRAEELALRKQVRDF"
     gene            594..941
                     /locus_tag="VFR57_02310"
     CDS             594..941
                     /locus_tag="VFR57_02310"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEU0258237.1"
                     /translation="MTQSNQERGRITARVPQDVQDTLEQAAELQGATLNQFVVQAALS
                     EARRVIERDRVIHLTSVDAAFLLNLLEKPPAPNARLRKAFRAYKKRTLDAEHSTFNWT
                     PRAARVRERKRAA"
     gene            871..1356
                     /locus_tag="VFR57_02315"
     CDS             871..1356
                     /locus_tag="VFR57_02315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0258238.1"
                     /translation="MLSIQPLTGRHERRAFESGSAQLDAWLRETAQQHQRRGISKTFV
                     ATAEEAPSRILGFYALTVCEVMADELPEDLARKLPRRVPGIRLGRLAVDRSVQGQGLG
                     ELLLMDAIKRAQLVLEHVGVNALFVDAKDSRAAAFYRKYGFRPLPGTPLTLVLALASV
                     S"
     gene            complement(1499..1630)
                     /locus_tag="VFR57_02320"
     CDS             complement(1499..1630)
                     /locus_tag="VFR57_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258239.1"
                     /translation="MAEAHSLSHRRRHPAGMLPLDLIVALAPLLVWLALCVLDDSGQ"
     gene            complement(1623..2591)
                     /locus_tag="VFR57_02325"
     CDS             complement(1623..2591)
                     /locus_tag="VFR57_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU0258240.1"
                     /translation="MDRLERFYKIDQLLKERKVVSFALFREKLGMSRASVKRDLEYMR
                     SRFNAPIEYDRHLNGYRFGRPRSGPRYELPGLWFNATEIYALLTTLQLLSNLQPGLLD
                     GQVKPVLERLRSILGTGDHSWEEVAKRMRIFLPERREGKAAHFSVIAAALLKRSRLWI
                     RHYNRKDDRETEREVSPQRLVHYRDNWYLDAWCHLRNDLRSFAVDAILDAVLKETPAK
                     EVREAELDEYLGSGYGIFAGRNVKWATLKFTPEAARWVSAQNWHPKQRVRVEKDGSYI
                     LELPYSEDRELVMEILKYGADVEVLAPPQLRKRVADSLRGAAQRYG"
     gene            complement(2638..2814)
                     /locus_tag="VFR57_02330"
     CDS             complement(2638..2814)
                     /locus_tag="VFR57_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258241.1"
                     /translation="MNKRPELYLIHAGEVPKERKLYRLRETRPHEAPAQVWPPDGEWR
                     ELVRRLTNNPVKSD"
     gene            complement(2811..3044)
                     /locus_tag="VFR57_02335"
     CDS             complement(2811..3044)
                     /locus_tag="VFR57_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein CsbD"
                     /protein_id="HEU0258242.1"
                     /translation="MEWSTIEGGWDEYKVAAKREWSKLSEQQLHGTRGNREYLLRRVQ
                     EAYSLSREDAERQISEFLAKQAEKRARKVGAQP"
     gene            3252..>4263
                     /locus_tag="VFR57_02340"
     CDS             3252..>4263
                     /locus_tag="VFR57_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0258243.1"
                     /translation="MRAGIGERVQRIEDPLLLRGEGRYTDDLNEPGQAYAYIVRSPHA
                     HGLLRGISLERAKAMPGVLAIYTASELAAYGPHKCNLDFKQRDGSAMRKPVRKSLAQD
                     KVRFVGDPVACVVAETYLQAKDAAEAVELDIEPLPAVTLPSEAAKPGAPQLYEDVPNN
                     VCLDFLWGEPEKVAPAFAGAAHITRLSLRNTRVVVAAMEPRAGICSFDKSNNNWTLTV
                     PGQGVWGQKTQLVDMLGVTPDKVRIRTYHVGGSFGMKGSIYPEYICLVHAARALGRPV
                     KWTDERSGSFLSDQHGRDHEMTGELALDKGGKFLALRITGYGNVGAYLGTVAPQPPSM
                     NVV"
BASE COUNT          727 a         1351 c         1433 g          752 t
ORIGIN      
        1 tggaaatccc tgacctgctt gcgtagcgcc agctcctcgg cgcggaactt ggcgagcgtg
       61 ccctccttcg cgctcaaagc ctcgttcagg gccttgagct ggatctcgaa ccgcgccttg
      121 gcttccgccg cgagttgggc ctcgagcttc ttcttgtgct cggcgagcga gcgctcgagc
      181 tcgcccgcgt ggcgctcgat cgcctggcgc gagatgcctt cggagagagg gaagcgatgg
      241 ctgcacttcg gacaaaacac cttttcttcg gcgtcgacga tgatggtgcg ggccatgcgc
      301 gcctccaaga aaatcggggt ctgaccccat gatgcgccgg gtagtggctc acagcgtgag
      361 cctgcgcggg actacctggg taaaggctaa ggcgaagtcg ccggggctcg acggctgggc
      421 cacgcattag cccgcattgc aggcggcgct aactccagca tatggagctt ggtgacaatt
      481 tggcaccaga atgctagtat ggagcttcaa acctcctaac ccagacgact taaagccaag
      541 acctccaaac tgtcaaccgg ccaaacaagc ccacgagcca ctgccaaacg cttatgaccc
      601 aatcgaacca ggaacgaggc cgaattacgg cccgggtgcc gcaggacgta caggacaccc
      661 tcgagcaggc ggctgagctg cagggggcga ccttgaatca gtttgtcgtg caggctgcgc
      721 tgagcgaagc acggcgcgta atcgagcgcg acagggtgat ccatctgacc agcgtcgatg
      781 cggcgttctt gctgaatttg ctggagaagc cgcccgcgcc taacgcaagg ctccgcaagg
      841 cgttccgggc atacaagaag aggacgctgg atgctgagca ttcaaccttt aactggacgc
      901 cacgagcggc gcgcgttcga gagcggaagc gcgcagcttg atgcctggct gcgcgagacg
      961 gcgcagcagc accagcggcg cggcatctcc aagactttcg tagccaccgc agaagaagcg
     1021 ccttcgcgga tactcgggtt ttatgcgctg acggtgtgcg aggtcatggc cgacgaactg
     1081 cccgaggatc tcgctaggaa gctgccgcgc agagtacctg gaatccggct tggccggctg
     1141 gccgtggatc gctccgtcca gggtcaaggc ctgggcgagc tattgctgat ggatgccatc
     1201 aagcgcgcgc agctcgtgct cgagcatgtc ggcgtgaacg ccctcttcgt cgatgcgaag
     1261 gactctcggg cggcggcgtt ctaccgcaaa tacggcttcc ggccgctgcc aggcactccc
     1321 ttgacgctcg tgctcgcgct tgcgagcgtg agctgagcgg tcagaccctc caaacctgct
     1381 ggcgcaagat tcggggtctg acctcctcgg tggctgggga tcgcgagcgg caatggactc
     1441 gagcttcctc acgatgcgac gcgcaaccag aggatcgagc ttcgagaggg tcttctctct
     1501 actgccccga atcgtcgagc acgcagagtg cgagccacac caggagcgga gcaagggcaa
     1561 cgatcaggtc gaggggaagc atgccggcag gatgccgccg gcggtggctc agggagtgag
     1621 cctcagccat agcgctgcgc cgctcccctc agcgaatcgg caacgcgctt gcgcagttgc
     1681 ggcggggcaa gcacttcgac gtccgccccg tacttgagga tctccatgac gagctcgcgg
     1741 tcctcggaat acggcagctc gaggatgtag ctgccgtcct tttccacgcg cacgcgctgc
     1801 ttcgggtgcc agttctgagc cgacacccag cgtgcggctt ccggggtgaa cttgagggtc
     1861 gcccacttca cgttgcggcc ggcgaagatg ccgtagccgc tgcccaggta ttcatccagt
     1921 tccgcctcgc gcacttcttt ggcaggagtt tccttgagga cggcatcgag gatggcgtcg
     1981 actgcgaagc tgcgcaggtc gttccgcagg tggcaccagg catccaggta ccagttgtcg
     2041 cggtagtgca cgaggcgttg cggggagacc tcgcgctcgg tttcgcggtc gtccttgcgg
     2101 ttgtagtgcc ggatccacag gcgcgatcgc ttgaggagcg ccgccgcgat gacgctgaag
     2161 tgcgcggcct tcccctcgcg ccgctcgggc aggaagatgc gcatgcgctt cgccacctct
     2221 tcccatgaat ggtcgcccgt gccgaggatc gagcgcagcc gctcgagcac cggcttcacc
     2281 tggccgtcga gcagccccgg ctgaagattc gagagtagct ggagcgtcgt gaggagcgcg
     2341 tagatttccg tggcgttgaa ccacaggccc ggcagctcgt agcgcgggcc cgagcgcggt
     2401 cttccgaagc ggtagccgtt gaggtgccgg tcgtactcga tcggcgcgtt aaagcggctc
     2461 ctcatgtact cgaggtcgcg cttcacgctc gcgcgcgaca tgccgagctt ttccctgaag
     2521 agcgcgaacg agaccacttt gcgctctttc aggagctggt cgatcttgta gaaccgctcg
     2581 agcctgtcca tgcgccccgt acgcgcgccg gcgaggccgg aatcttactg cctggcgcta
     2641 gtcgctcttg accgggttgt tcgtcagccg gcgcacgagc tcgcgccact caccgtccgg
     2701 cggccagact tgcgcaggag cttcgtgcgg acgggtttcg cgcaggcggt agagctttcg
     2761 ctccttcggc acttctcccg cgtggatcag gtaaagctcg gggcgcttgt tcatggctgc
     2821 gcgcccactt tgcgcgcgcg cttctccgcc tgcttcgcga ggaattcgga gatctggcgc
     2881 tcggcgtctt ccctgctcag cgaataggct tcctgcacgc ggcgcaacag gtactcgcgg
     2941 ttgccgcgcg tgccgtggag ctgctgctcg gagagcttgc tccactcgcg cttggcggcg
     3001 accttgtatt cgtcccagcc gccttcgatg gtgctccact ccaatgctgc ctccgttcgc
     3061 gttgctcgag ctgcggaccc gcggcgaggc gggcgatgcg gattgctgct gcgtagcggg
     3121 agggactgaa ggatcgccgg caaggcggcg gcgccacagg ctgcttccgg aacacggcca
     3181 ccatactggc cgctcccccg ggcggtcaag gcatttctgg gaagcggcgc gcgtttgcaa
     3241 taattcggcc catgcgagct ggaatcggcg agcgcgtaca gcgcatcgag gaccctttgt
     3301 tgttgcgcgg cgaaggccgc tacaccgacg acctgaacga gcccgggcag gcgtacgcgt
     3361 atatcgtgag gagcccacac gcgcatggcc tgctcagggg aatttctctg gaaagggcca
     3421 aagcaatgcc gggggtgctc gccatctaca cggcatccga gctggcggcg tacgggccgc
     3481 acaagtgcaa cctcgacttc aagcagcgcg acggttcggc gatgaggaag cccgtccgca
     3541 agtcgctcgc ccaggacaag gtgcgcttcg tgggcgaccc ggtggcgtgc gtggtggccg
     3601 agacgtacct gcaggcgaag gacgcggcag aggcggtgga gctcgacatc gagccgctgc
     3661 cggcggtgac cctgccctcc gaggcggcga agccgggcgc gccgcagctc tacgaagacg
     3721 tgccgaataa cgtctgcctc gactttctct ggggcgagcc ggagaaggtg gcccccgcct
     3781 tcgcgggggc ggcacacatc acccgcctgt ctcttagaaa tacgagggtg gtggtggcgg
     3841 cgatggagcc gcgcgcaggg atttgttctt tcgataaatc gaacaacaac tggaccctga
     3901 cggtgcccgg ccagggcgtc tggggccaga agacgcagct cgtcgacatg ctcggcgtga
     3961 cgccggacaa ggtgcgcatc cgcacctacc acgtcggcgg ctcgttcggg atgaagggct
     4021 cgatctaccc ggagtacatc tgcctcgtgc acgcagcccg cgcgctcggg cggccggtga
     4081 agtggacgga cgagcgctcg gggtcgttcc tctcggacca gcacggccgc gaccacgaga
     4141 tgacgggcga gctggcgctc gacaagggcg gcaagttcct cgcgctgcgc atcacgggct
     4201 acggcaacgt cggcgcatac ctcggcacgg tggcgccaca gccgccgtcg atgaacgtgg
     4261 tgc
//
LOCUS       DASTPD010000059         5477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_232207, whole genome shotgun sequence.
ACCESSION   DASTPD010000059 DASTPD010000000
VERSION     DASTPD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232207"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFR57_02345"
     CDS             complement(<1..486)
                     /locus_tag="VFR57_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEU0258244.1"
                     /translation="MPRIIRIVLAPRDEWARIAAERSHGALAHGLALSLLPALASLAM
                     AWSSDLPLPEHAVAATYLACVLTIFAIAAAFWLLARFDSRDATLRRCVQVAAYGATPV
                     LLASVLLFTPVLVLVCVLALPYVFYLYYLGVQQVLRVPSDEAAQFVAVALVAALVAST
                     LG"
     gene            476..1642
                     /locus_tag="VFR57_02350"
     CDS             476..1642
                     /locus_tag="VFR57_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0258245.1"
                     /translation="MRGIKSILTARRAAEIEPFEVMDVLSRAQALERAGRRVVHMEIG
                     EPDFTAPAPVVEAGIRALRDGRTGYTATLGLASLREAICGHYAARFGAQVPAERIAIT
                     CGASGALLTVAALYVDAGDEILVPDPGYPGYRHFVRAFEGTANTLPVAAQSDFQPTLD
                     MVRAAWGPRTKGLLLGSPSNPTGTLISKEELRKIAGFIAERGGVLLVDEIYQGLVYGI
                     EPWTAFGLPGDVVLINSFSKYFCMTGWRLGWVVLPVAALRGFEKLAQHLFISAPAAAQ
                     HAAASAFSSASLSILEQRRSEFARRRDFLVPQLREAGLSIPAEPRGAFYVYADCGGDA
                     RRFAKGLLESEAVAATPGVDFGANGTDRFVRFAYTRSMDDLEEAARRIARFCEP"
     gene            complement(1611..2297)
                     /locus_tag="VFR57_02355"
     CDS             complement(1611..2297)
                     /locus_tag="VFR57_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0258246.1"
                     /translation="MKARSMTQHLSAALNALKELLALVGVAAILTVLFFFPHRPHVEK
                     ALSSVVIDQLIEAPGRTDARVKVSMVEVVLNREQQVVAEFIAKRYRVAEDAAGEFVAL
                     AYRAGAQFSLDPLLILAVMAIESSYNPIAQSVMGARGLMQVIPKYHPEKLEPHGGEQA
                     LLEPTVNILVGAQILREYQRRLGDTEAALQMYAGAFDEPTSQYANKVFAEKARLEALR
                     LKARKSGQSA"
     gene            complement(2670..2963)
                     /locus_tag="VFR57_02360"
     CDS             complement(2670..2963)
                     /locus_tag="VFR57_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258247.1"
                     /translation="MAKKKAKKGGKKKKKKKASRKKKKSLRLAGKKKAAGKKKSAKKK
                     KKAGKKKKATEAAPPASFADTAAAETPSTAATPGARPALNPAAAWPFPTGSRP"
     gene            complement(3014..3313)
                     /locus_tag="VFR57_02365"
     CDS             complement(3014..3313)
                     /locus_tag="VFR57_02365"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEU0258248.1"
                     /translation="MLEELDTMRITACKADEDYKLWLRFEDGLEGRVFLGNLLEIGAF
                     QLWRDVREFEKVSVDPETATVTWEGGIRLDADILYHDVKANQIQRAGGDEPQAVE"
     gene            complement(3339..4448)
                     /locus_tag="VFR57_02370"
     CDS             complement(3339..4448)
                     /locus_tag="VFR57_02370"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEU0258249.1"
                     /translation="MLTFEESISLPASGVPAPGGAARTALPSEAFRRVTVEDKRVINS
                     KADVNQLVPFKYKWAWEKYLAACANHWMPQEINMSRDIGLWKDPAGLTEDERRIVKRN
                     LGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLDLDEA
                     EIFNAYHEIPSIREKDEFLIPFIDTLTDPAFTTGSPENDQKLLKSLIAFSCLMEGLFF
                     YVGFAQILSLGRQNKMTGAAEQYMYILRDESMHCNFGIDLVNQIKLENPHLWSPAFRE
                     ELQALFRRAVELEYRYAEDTMPRGVLGLNAPMFKEYLRFIANRRCQQIGLDALYPGAA
                     NPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD"
     gene            complement(4656..>5477)
                     /locus_tag="VFR57_02375"
     CDS             complement(4656..>5477)
                     /locus_tag="VFR57_02375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559784.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU0258250.1"
                     /translation="ASPEAVEFADRSMEMVAYAAYWASTELAEERGRYASFPGSLWDR
                     GILPQDTLDILSAERGGYVELDRSARLDWERLRARIKQHGMRNSNCLAIAPTATIANI
                     VGVSASIEPTYQNLFVKSNLSGEFTVVNDYLVADLKQAGLWDEVMIADLKYFDGNLAR
                     IDRAPAELRRLYATAFEIEPKWLVECAARRQKWIDQSQSLNIYMAGDSGKRLDETYKL
                     AWIRGLKTTYYLRALGATHAEKSTAKAGELNAVPAESLDAPKMCAIDQPDCEACQ"
BASE COUNT          902 a         1763 c         1760 g         1052 t
ORIGIN      
        1 gccgagcgtt gaggccacga gggcagccac cagcgcgacc gcgacgaact gcgccgcctc
       61 atcggaaggc acgcgcagca cttgctgcac ccccaagtag tagagataga agacgtacgg
      121 gagagcgagc acgcacacta gcaccagcac gggagtgaac agcagcaccg aagcaaggag
      181 gacgggcgtc gcgccatacg ccgcgacttg gacgcagcgc cgaagagtcg catcccgcga
      241 atcaaaccgc gcgagcagcc agaaagccgc tgcgatggcg aagatggtga gcacgcacgc
      301 aaggtaagtg gcggccaccg catgctcggg aagcggcaag tcggagctcc acgccatcgc
      361 cagggaagca agcgcgggca gcaacgacaa tgcgaggccg tgggccagcg cgccatgcga
      421 gcgttcggcc gcgatgcgtg cccactcgtc tctcggcgcg agcacgatcc gtataatgcg
      481 cggcatcaaa tccattctaa cggcgcgccg cgccgccgag atcgaaccct tcgaggtgat
      541 ggacgtgctc tcgcgcgcgc aagcgctcga gcgcgccggc cgccgcgtgg tacacatgga
      601 gatcggcgag ccggatttca ccgcgcccgc gccggtcgtc gaggcaggga tccgcgcgct
      661 gcgcgacggt cgcaccggct acaccgccac gctcgggctc gcgtcgctga gggaggcgat
      721 ctgcgggcat tacgcggcgc gcttcggcgc acaggtcccg gccgaaagaa tcgccattac
      781 ctgcggcgcc tcgggcgcgc tactcacggt ggccgcgctc tacgtggatg ccggcgatga
      841 gatcctggtg cccgatccgg gctacccagg ctaccgccac tttgtgcgcg cgttcgaagg
      901 cacggcgaac actctgccgg tcgcagcgca gagcgatttc cagcccaccc tcgacatggt
      961 gcgcgccgcc tggggtccgc ggaccaaggg actcctcctt ggctcgcctt cgaatcccac
     1021 cggaacactg atctcgaagg aagagcttcg caaaatcgcg gggttcatcg ccgaacgtgg
     1081 cggcgtgctg ctggtcgacg agatctacca gggcctggtg tacggcatcg agccatggac
     1141 cgcgttcggc ctgcccggcg acgtggtact gatcaacagc ttttcgaagt acttctgcat
     1201 gaccggctgg cgcctcggat gggtggtgct gccggtagcg gcgctgcgcg ggttcgagaa
     1261 gctcgcgcag catctcttca tcagcgcgcc ggccgccgca cagcatgcgg ctgcttcagc
     1321 tttttcttcc gcaagccttt cgattctgga acagcgcaga agcgaattcg cccgcagacg
     1381 cgatttcctg gtgccgcagt tgagggaggc gggactttcg atcccggccg agccgcgcgg
     1441 tgctttctac gtgtacgccg actgcggcgg cgacgcgcga cgcttcgcga aaggcctgct
     1501 cgagagcgaa gcggttgccg cgaccccggg ggtggatttc ggcgcgaacg gcaccgaccg
     1561 attcgtgcgc ttcgcctaca cgcgaagcat ggacgatctc gaggaggccg ctaggcggat
     1621 tgcccgcttt tgcgagcctt gagccgcagc gcctcgaggc gcgccttttc cgcgaagacc
     1681 ttgttggcgt actgagaagt cggctcgtcg aaggcgccgg catacatctg cagggcggcc
     1741 tcggtgtcgc ccaggcggcg ctggtattcc cgcaggatct gggcgccgac caggatgttc
     1801 accgtcggct cgagcagcgc ctgctccccg ccgtgcggct cgagcttttc cgggtggtac
     1861 ttcgggatga cttgcatcag gccgcgcgca cccatgacgc tttgcgcgat cgggttatag
     1921 ctcgactcga tcgccatgac ggccaagatc agcagcgggt cgagcgagaa ctgggcgccg
     1981 gcgcggtagg cgagcgccac aaactcaccg gccgcgtctt ccgccacccg gtagcgcttg
     2041 gcgatgaatt cggccaccac ctgctgctcg cggttgagga ctacctcgac catactgacc
     2101 ttgacccgtg cgtccgtgcg gccgggcgcc tcgatgagct gatctatcac taccgaagac
     2161 agggcttttt ccacatgtgg gcgatggggg aagaagaaca gaacagtgag gatcgccgcc
     2221 accccgacca gagcaagcaa ctccttgagc gcgttcagcg cggctgaaag gtgctgcgtc
     2281 atgcttcgtg ctttcatctg tgcctcccgt cttgcggtcg tgaagtgtgc ccttccaggg
     2341 cctgcaccgg aagccttgct gttgcattgc agcgagccgc gatgatatta gcttttctct
     2401 ttacgagtca agaacttcta attctttcgg caggcccaaa aaaccctgcc acaagcgtgg
     2461 tttcgacaac atttttcatg ttggtggtgt ggaaaacgcc ccgtaagatg aattcaccca
     2521 gaagccccgt tcaccagggg attcggggct ctcaggaagc ctcgcggacc ggcccaagtc
     2581 tccgttcttg ggccgaatga ccaagccggt ccgctgagct tcgggccggc ggaggcttgc
     2641 gcctccgcca cgttcccgcg cctctccggt tacggccggg agccggtggg aaacggccac
     2701 gcggccgctg gattcagcgc gggtctcgcg ccgggggtcg cagcggtgga cggcgtctct
     2761 gcagccgccg tgtccgcgaa gctggccggt ggggcggctt ctgtcgcctt tttcttcttc
     2821 cccgcctttt tcttcttctt ggcggatttc tttttccctg ccgccttttt cttacccgct
     2881 agtctcaacg acttcttttt ctttcttgat gctttcttct tcttcttttt cttgccgcct
     2941 tttttcgctt ttttcttcgc cattctcaat ctccttgaag gttgttgaac aaacgagccc
     3001 gggcaggccc ggactattca accgcctgcg gctcgtcgcc gccggcgcgc tgaatctgat
     3061 tcgccttcac gtcatgataa agaatatccg cgtcgaggcg aatgcctccc tcccacgtca
     3121 ccgtcgccgt ctctgggtcg acactcacct tctcgaactc gcgcacatcg cgccacagct
     3181 ggaaggcgcc gatctcgagc aggttgccga gaaacacccg gccttccagg ccgtcctcga
     3241 agcgcagcca cagcttatag tcttcgtccg ccttgcaggc agtgattcgc atcgtgtcca
     3301 gctcctcaag catgttgctc cccctctacc ttgaatgctc aatcccagct aagcgctccc
     3361 ccggtctggt actcgatcac ccgggtctcg aagaagttgc gttccttctt caggtcgatc
     3421 atctcgctca tccaagggaa cgggttcgcg gccccgggat agagcgcatc caggccgatc
     3481 tgctggcagc gccggttggc gatgaagcgc aggtactcct tgaacatggg cgcgttcagc
     3541 cccagtacgc cgcgcggcat ggtgtcctcg gcgtagcggt actcgagctc gacggcgcgg
     3601 cggaacaggg cctgcagctc ctcgcggaaa gccgggctcc acaggtgcgg gttctcgagc
     3661 ttgatctggt tgacaagatc gatgccgaaa ttgcagtgca tcgactcgtc gcgcaggatg
     3721 tacatgtact gctccgcggc cccggtcatc ttgttctgcc ggccgagcga aagaatctgc
     3781 gcgaagccga cgtagaagaa cagtccctcc atcaggcagg agaaggcgat caggctcttc
     3841 aggagcttct ggtcgttctc gggcgagccg gtggtaaagg ccgggtcggt gagggtgtcg
     3901 atgaagggga tcagaaactc gtccttctcc cggatcgacg ggatttcgtg gtaggcgttg
     3961 aagatctcgg cctcgtccaa gtccaggctt tcgacgatgt actggtaggc gtgggtgtgg
     4021 atcgcctcct cgaacgcctg gcgcagcagg tactgccggc attcgggcgc ggtgatgtgc
     4081 cggtaggtgc cgagcacgat gttgttcgcc gcgagggagt ctgcggtcac gaagaagccg
     4141 agattgcgtt tcacgatcct gcgctcgtcc tcggtcaggc cggccgggtc cttccacagc
     4201 ccgatgtccc ggctcatgtt gatctcctga ggcatccagt ggttcgcgca ggccgccaag
     4261 tacttctccc acgcccactt gtacttgaag gggaccagct ggttgacgtc ggccttggag
     4321 ttgatgacgc gcttgtcttc taccgtgacg cggcggaacg cctcgctcgg caatgcagtg
     4381 cgggcggcgc cgcctggcgc gggaaccccg ctcgccggga gggaaatgct ttcttcgaaa
     4441 gtgagcatgg caggcctggg ctgttctagt tgtacttgtg ggtgcgcagc gccttcgtca
     4501 tcgcgagcgg cttgaattcc cggcggtacc ccgcgtgcga gctcccgcaa gagcgtgccg
     4561 caagccgtgg gggtggacag agccatctcg tgcgttgcga cgtaggggct ccactcgctc
     4621 gggccgcgag cgcgatcacg tgcccgcccc gagaatcatt gacatgcctc gcagtccggc
     4681 tggtcgatcg cgcacatctt cggcgcgtcg aggctttcgg ccggaacggc gttgagttct
     4741 ccggcctttg ccgtggactt ctccgcgtgc gtggcgccga gcgcccgcag gtagtacgtc
     4801 gtcttcaggc cgcggatcca ggcgagcttg taggtctcat ccagcctctt gcccgagtcg
     4861 cccgccatgt agatgttgag cgactgcgac tggtcgatcc acttctggcg ccgcgccgcg
     4921 cactcgacca gccacttcgg ctcgatctcg aaggcggtgg cgtagagccg gcgcagctcg
     4981 gcgggcgcgc ggtcgatgcg tgccaggttg ccgtcgaagt acttgagatc ggcgatcatg
     5041 acctcgtccc acaatccggc ctgcttcagg tcggcgacca ggtagtcgtt caccaccgtg
     5101 aactcgcccg aaaggttgga tttcacgaac aggttctggt aggtcggctc gatcgaggcc
     5161 gacacgccga cgatgttggc gatggtggcg gtgggtgcga tggcgaggca gttggagttg
     5221 cgcatgccgt gctgcttgat gcgcgcgcgc agccgctccc aatcgagccg cgcggagcgg
     5281 tcgagctcca cgtagccgcc gcgctcggcc gacagaatgt cgagcgtgtc ctgcggcagg
     5341 atgccgcggt cccagagcga ccccgggaac gaggcgtagc ggccgcgctc ctcggcgagc
     5401 tcggtcgacg cccaataggc cgcgtacgcc accatttcca tcgagcggtc ggcgaactcg
     5461 accgcctccg gcgatgc
//
LOCUS       DASTPD010000060         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_436145, whole genome shotgun sequence.
ACCESSION   DASTPD010000060 DASTPD010000000
VERSION     DASTPD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436145"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="mreC"
                     /locus_tag="VFR57_02380"
     CDS             complement(<1..856)
                     /gene="mreC"
                     /locus_tag="VFR57_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU0258251.1"
                     /translation="MDHTPPPFFKRGPAPLVRLFFFASLSLALLVIDARFRYADGLRT
                     WLALGAYPLQRAATAPLELANALGAFFSTQAQLVAENERLRAKALAYSQDAQRYEASQ
                     AEAEQLRRLAGAQQRMPVRVVTAEVMYNGRDPYAHKLFIDRGAAHGVRPGSPVTDESG
                     VVGQVTRVHALVSEVTLATGRDQAIPVQVVRNGLRAVAFGGGASGLLELRFMAANADI
                     QTNDRLVTSGIDGTYPAGLPVATVVRIERDAENAFARVVCRPAAGVDRGRFVLVLSDE
                     TVKPPRPEE"
     gene            complement(869..1912)
                     /locus_tag="VFR57_02385"
     CDS             complement(869..1912)
                     /locus_tag="VFR57_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0258252.1"
                     /translation="MLGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     AGPSGKKTIQAVGKEAKQMLGKTPGNIMAIRPMKDGVIADFTVTEQMLKQFIKKVHDN
                     KLFSPSPRIIICVPCGSTQVERRAIRESAMGAGAGQVYLIEEPMAAAIGADLPVADAT
                     GSMVVDIGGGTTEVGVISLGGMVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAEL
                     IKKEIGSAFPGSEVREVEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADDPLTCVVRGSGKAL
                     EKMEHFGPIFTND"
     gene            2070..2357
                     /gene="gatC"
                     /locus_tag="VFR57_02390"
     CDS             2070..2357
                     /gene="gatC"
                     /locus_tag="VFR57_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0258253.1"
                     /translation="MSLSDDQVRRLARLARIAIRAEEFEPVLDRLNRVLGLVDEMRQV
                     DTAGMEPMAHPLDATQRLRPDIIIESDRRELYQSVAPAVEGGLYLVPKVIE"
     gene            2374..3840
                     /gene="gatA"
                     /locus_tag="VFR57_02395"
     CDS             2374..3840
                     /gene="gatA"
                     /locus_tag="VFR57_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU0258254.1"
                     /translation="MLNSSLTELSAFLAARNISSVELTGLFLDRIAKLNGDLNAFIWP
                     EKTRDVALARARAADDRRAAGDAGPLTGIPIAFKDIYCTKGVHTTCGSRMLENFVSPY
                     DAHVVEQFGRAGAVLLGKCNMDQFAMGSSNESSYFGPVRNPWARSRVPGGSSGGSAVA
                     VAARMAPAATGTDTGGSTRQPAAFSGICGLRPTYGLVSRYGLVAFASSLDQAGPLARS
                     AADLALLLNVMAGFDARDSTSVDRPKEDYTRALDADLKGLRIGIPREYFAEGLEPGVR
                     VAVEQAVSWFISQGGVRVELQLPLTRLAVPAYYVIAPAEASSNLSRFDGVRYGHRAHE
                     HADLADMYCRTRAEGFGDEVKRRILVGTYVLSHGYYDAYYLQAQKVRRLIVQDLSRAF
                     ERCDVILGPTAPTTAFEIGAQAEDPVQMYLGDIFTVPAPLAGLPAVSIPCGFDAAGLP
                     VGLQLMGRHFSEGLLLRAAHRYQQATDWHLRVPTETPL"
     gene            3837..>4118
                     /locus_tag="VFR57_02400"
     CDS             3837..>4118
                     /locus_tag="VFR57_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258255.1"
                     /translation="MRLIFGVALLAVSWAALPQNKAERHELIGKVGTRTALLTLHAGR
                     RTDGSWQVAGEYVVLATLARRFVEGESSPELGVTVLKEGTTPILFGRPPI"
BASE COUNT          667 a         1350 c         1345 g          756 t
ORIGIN      
        1 tttcttcggg acgcggcggc ttgactgtct catcggagag cacgagcacg aagcgcccac
       61 ggtcgacgcc ggcggcgggc cgacacacca cgcgggcgaa agcgttctcc gcgtcgcgct
      121 cgatgcgcac taccgtggcg accggtagtc cggcggggta ggtgccatcg atacccgagg
      181 tgaccaggcg gtcgttggtc tggatatcgg cgttcgccgc catgaagcgc agctcgagca
      241 atcctgaagc gccgccaccg aacgccaccg cccgcagtcc gttccgcacc acctgcaccg
      301 ggatcgcctg gtcgcggccg gtggcgagcg tgacctccga aacgagtgca tgaacccgtg
      361 tcacctggcc aacgacgccc gattcgtcgg tcaccgggct gccgggacgc acgccgtgcg
      421 ccgcgccgcg gtcgatgaac agcttatgcg cgtacgggtc acgtccgttg tacatcacct
      481 cggcggtaac gacccgcacc ggcatgcgct gctgcgcgcc ggcaagcctg cgcagctgct
      541 cggcttcggc ctgcgatgcc tcgtagcgct gcgcgtcctg cgagtaggcg agcgccttgg
      601 cgcgcaggcg ctcgttctcc gcgacaagct gcgcctgagt cgagaagaaa gcgccgagcg
      661 cgttcgcgag ctcgagcggc gcggtggcgg cgcgttgcag cggataggcc ccgagcgcga
      721 gccacgtacg cagcccgtcg gcgtagcgaa agcgtgcgtc gatcaccagc agcgcaagcg
      781 atagcgaagc aaagaagaac agcctgacga gcggcgccgg tcctcgcttg aagaagggcg
      841 ggggggtgtg gtccatcccc ctttacacct aatcgttcgt gaagatcggg ccgaagtgct
      901 ccatcttctc gagcgccttg ccggagccgc gtaccacgca cgtgagcggg tcgtcggcga
      961 cgatcaccgg cagcccggtc tcttccatca ggaggcgatc gaggtcgcgc aacagcgcgc
     1021 cgcccccggt gagcaccatc cccttctcgg cgatgtctgc gccgagctcg ggcggtgtct
     1081 gctcgagggc cgacttcact gccgaaacga tctggttgag cggctcggta agcgcttcaa
     1141 ggatctcgtt cgagctgatg gtgaagctcc tcggaatgcc ctcggcgagg ttgcggccct
     1201 tgacttcgac ttcgcgtact tccgagccgg ggaacgccga gccgatttct ttcttgatca
     1261 gctccgcggt cgtttcgccg atcagcatgc cgtaattgcg gcggatgtag ttgatgatcg
     1321 cttcgtcgaa cttgtcgccg ccgacgcgca ccgagcccga ataaaccatg ccgccaaggg
     1381 agatcacgcc cacttcggtc gtgccgccac cgatgtcgac gaccatcgag ccggtggcat
     1441 cggccaccgg cagatccgca ccaatggcgg ccgccatcgg ttcttcgatc aggtacacct
     1501 gtccggcgcc ggcgcccatc gcgctttcgc ggatcgcgcg gcgctcgacc tgggtggagc
     1561 cgcacggcac gcagatgatg atgcggggcg agggcgagaa cagcttgttg tcgtggacct
     1621 tcttgatgaa ctgcttgagc atttgctcgg tgacggtgaa gtcggcgatg acgccgtctt
     1681 tcatcggccg gatcgccatg atgttgcccg gcgtcttgcc gagcatctgc tttgcttcct
     1741 tgccgacggc ttggatggtt tttttaccgc tagggccggc ttcctgtctg atcgcgacga
     1801 cggagggctc gtcgagcacg atgcctttgc cgcgtacgta gatcagtgta ttcgcggtgc
     1861 ccaggtcgat cgcaaggtcg ttggaaaagt agctgcgcag gaatccaagc ataggcaaag
     1921 tcgaagcctt tctccgttcg tgccgtttta tcgttagcta tccgggcctt aggtgtccta
     1981 cctcaagccc gtttcaatgg ctcgctatga tacgctaacg acgctgtggg agataaggca
     2041 atgctccccg gcttcccagt ccgagaagaa tgtcgctatc cgacgaccag gtgcggcgcc
     2101 tcgcccggct cgctcgcatc gccatccgcg ccgaggagtt cgagccggtg ctcgaccggc
     2161 tgaaccgggt gctcggcctg gtggacgaga tgcgccaggt cgacactgcc ggcatggagc
     2221 ccatggcgca ccccctggac gcgacccagc gtcttcgccc ggacatcatc atcgaatcgg
     2281 atcggcgcga gttgtaccag tcggtcgccc cggcggtgga agggggcctt tacctcgtcc
     2341 ccaaggttat agagtgaaag ccgagtaggc gtagtgctta attcgagcct caccgagctc
     2401 tccgccttct tggcggcgag gaatatttcc tcagtcgagt tgactggcct cttcctcgac
     2461 cgcatcgcga agctcaatgg cgacttgaat gccttcatct ggccggagaa aacgcgcgat
     2521 gtcgccctcg ctcgggcccg tgccgccgat gatcgccgcg ccgccggaga tgccgggccg
     2581 ctcactggca tcccaatcgc gttcaaggac atttattgca ccaagggcgt ccatacgacc
     2641 tgcggctcga ggatgctgga aaatttcgtg agcccgtacg acgcgcacgt ggttgagcag
     2701 ttcggccgcg ccggggcggt gctgctgggc aagtgcaaca tggaccagtt cgccatgggc
     2761 tcgtcgaacg aaagctccta tttcggcccg gtacgcaatc catgggcgag aagccgcgta
     2821 ccgggcggct cttccggagg ctcggcggtg gccgtcgcag cgcgcatggc gcccgcggcg
     2881 accgggaccg ataccggcgg ctcgacccgt cagcctgcgg cgttcagcgg catctgcggc
     2941 ttgcggccga cctatgggct ggtctcgcgc tacggtctgg tggcattcgc ctcgtccctc
     3001 gaccaggccg gacccctcgc gcgcagcgcc gcggatctcg ccttgctcct caacgtgatg
     3061 gccggcttcg acgctcggga ctcgaccagc gtcgatcggc cgaaagaaga ttacacaagg
     3121 gcattggacg cggacctgaa aggcctgcgt atcggcatcc cgcgcgagta tttcgccgaa
     3181 ggcctcgagc cgggcgtgcg cgtggccgtc gagcaagccg tctcgtggtt catctcccag
     3241 ggaggggttc gtgtcgagct gcaattgccg ctaacgcggc tggccgtgcc ggcgtactac
     3301 gtcatcgcgc cggccgaagc gtcgtctaac ctgtctcgct tcgacggcgt gcgctatggc
     3361 caccgagcgc acgagcacgc ggatctggcc gatatgtatt gccgcacgcg cgccgaaggc
     3421 ttcggcgacg aagtgaagcg ccgcatcctg gtgggcacgt acgtgctgtc gcacggctat
     3481 tacgacgcct actacttgca ggcgcagaag gtgcgccgtc tcatcgtgca ggacctttct
     3541 agagcgtttg agcgctgcga cgtgatcctt ggtccaacag cgccgaccac ggcattcgaa
     3601 attggcgcgc aggcagagga cccggtgcag atgtacctcg gcgatatctt cactgttccg
     3661 gcgccgctcg cagggctccc agcggtgtcg attccctgcg gcttcgatgc cgctgggctg
     3721 ccggtcggcc tgcagctcat gggacggcat ttttccgaag ggctcttgct ccgcgccgcg
     3781 caccgttacc agcaggcgac cgactggcat ctgcgcgtgc cgacggagac gccgttgtga
     3841 ggctcatctt tggggtcgcc ttgctcgccg tttcgtgggc tgcccttccc cagaacaagg
     3901 ccgaaagaca cgagctcatc ggcaaagtcg gcaccaggac cgcgctcctc acccttcacg
     3961 cggggcggcg caccgacggc tcgtggcagg tggccggcga gtacgtggtg ctcgccacgc
     4021 tggcgcggcg cttcgtcgag ggcgaaagca gcccggagct tggcgtcacg gtcctgaagg
     4081 aaggcaccac gcccatcctc ttcggccgac cgccgatc
//
LOCUS       DASTPD010000061         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_844964, whole genome shotgun sequence.
ACCESSION   DASTPD010000061 DASTPD010000000
VERSION     DASTPD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844964"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..864)
                     /locus_tag="VFR57_02405"
     CDS             complement(172..864)
                     /locus_tag="VFR57_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258256.1"
                     /translation="MSPQPTERIRVLLVDRHQIILWGVKQLIEANKPGMEVVGTAADW
                     ASAIAAAKETWPHVVVLDSQMLRVNGAGTIRSLIEAGNAKVLLFSGEQDDLLHETAIL
                     QGASGVVRKDETPSVLLKAITKVHDGQLWLDRSTTGRMFVQFSRRKRDGAEDMARKKL
                     ETLTEREYDVVRTLVTQPGADNKRLARNLHIGEHTLRNHLSRIYDKLGVPNRVELYLF
                     AQRHGGPGQGSH"
     gene            1039..1590
                     /locus_tag="VFR57_02410"
     CDS             1039..1590
                     /locus_tag="VFR57_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEU0258257.1"
                     /translation="MRPSLLTLRVGFALPALACAALLGFGYYLQYVEGLNPCPLCLVQ
                     RGFFYAVAGTCVIASIHAPRGWGRTAYGLVAALFAAGGFATAARQVWLQHLPPDKVPQ
                     CGPDLYFMFENFPLSRVLKTLITGTGECAVVDWTFLGLSIAEWSLGCFAALFVYALWL
                     ALRRPKAGAAADVSFPEVEGKFR"
     gene            complement(1503..2354)
                     /gene="cobA"
                     /locus_tag="VFR57_02415"
     CDS             complement(1503..2354)
                     /gene="cobA"
                     /locus_tag="VFR57_02415"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU0258258.1"
                     /translation="MTVGVGHHGKKSGSDPDWVGTVYLVGAGPGAPDLLTVRAARLLA
                     EADIVFYDALVHPETLGLAARAEKIAVGKRCGRHSTAQRFINKRLAHAARRHRVVVRL
                     KGGDPMLFGRAHEEISFLKENSINVEVVPGITAALAASAELGVSLTQRLVSRSVVFAT
                     PRTVAGAEPSGWEKAVAAADTAVLYMAGGEAEAVKEALLRQGMSATTPVAIAENVSLD
                     SSSMRRGNLAALPDLAAALEGGAALVLIGEVFAAREAQRNLPSTSGKLTSAAAPAFGL
                     RSASQSA"
     gene            complement(2351..2728)
                     /locus_tag="VFR57_02420"
     CDS             complement(2351..2728)
                     /locus_tag="VFR57_02420"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEU0258259.1"
                     /translation="MATLIKGRRVVAESRPWPEEKVLRLEPADDPASISLEGVARVEV
                     NFPKFGDGRGYSIARLLRERYGYRGELRAVGHITRDLLLFLESCGFDAFELRDGEDPH
                     EALASFEDFSHSYQRGYRAGVRP"
     gene            complement(2673..>4004)
                     /locus_tag="VFR57_02425"
     CDS             complement(2673..>4004)
                     /locus_tag="VFR57_02425"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEU0258260.1"
                     /translation="IQTSGNSFRNVTTDHFAGVAVDEIVDSFVWCEILRQWSTFHPEF
                     SFLPRKFKIAVNGARADRAAVGVHDIGLHAIRKGEQIGFRVLVGGGLGRTPMIGVAIR
                     EFLPWPHLLTYVEAILRVYNRHGRRDNIHKARIKILVKERGADRFRDEVEAEWAHVKD
                     GPATLTAEDIARVEQRFSRPQYRKSGSEPDFSEPGFVAWKKRNVQAHKVPGYAAVTLS
                     LKKTGVPPGDVTSEQMDAIANLAERYSFGELRVSHEQNLVLADVRRRDLPALWRETRE
                     LGLATPNIGLLTNIISCPGGDYCALANAKSIPVAQAIQERFDDLDYLHEIGELDLNIS
                     GCINACGHHHVGHIGILGVDKNGEEWYQIEVGGNQAPASLGKIIGPAFARAEVPDVIE
                     RLIGCYLQHRDSEAERFVDVVHRIGIEPFKEHVYGHAHQGAARRSREPALA"
BASE COUNT          643 a         1291 c         1279 g          791 t
ORIGIN      
        1 catgacccga ttaacagttg gccgcatcga tgttaacgac ggggtaagcg cgctgcatat
       61 cgcagccgcg gcgatcttgt tgtagccgat cggcgtcggc cggcggccgg cgcgctacgt
      121 cttgcctatg ctgcgcgccg gcgttcccca agctccgggg tttcgttctt cttagtggct
      181 tccttggccg ggtccgccgt ggcgctgcgc gaatagatac agctcgacgc gattcggcac
      241 gccgagcttg tcgtaaatgc ggctgagatg gttgcgcagc gtgtgctcgc cgatgtgaag
      301 attgcgcgcg agcctcttat tgtccgcgcc tggctgcgta acgagcgtcc gaactacatc
      361 gtactcgcgc tcggtgagcg tttcgagctt tttcctggcc atatcttcgg cgccatcgcg
      421 tttgcggcgc gaaaattgga cgaacatgcg gccggtggtg gagcgatcga gccagagttg
      481 gccgtcgtgt accttcgtga ttgccttcag gaggaccgac ggcgtttcgt ccttgcgcac
      541 tacgccggag gcaccctgga gaatggcagt ctcgtgcagc aggtcgtcct gctcgccgct
      601 gaagaggagc accttagcgt tgcccgcctc gatcaggctc cgaattgtac cggcgccgtt
      661 gacgcgaagc atttgcgagt cgagcactac cacgtgcggc catgtttcct tcgcggccgc
      721 gatagcgctg gcccaatctg ccgcggtccc tacaacctcc atgcccggct tgttggcctc
      781 tattagctgc ttcacccccc acaagataat ttggtggcgg tcaaccagca gtacacggat
      841 ccgttcagtc ggctgcggac tcattgtttc gctcaagggc cgatgatagc gaacaaagat
      901 taagagaagc agtctttgcc cgaacccgat aagccgtaca agacactttt ttcaagccac
      961 ttgtcgcagg atttacaaga gattttttag gaaccccggc taaatgcacg ccgtatttgg
     1021 gataatttgg tcaaagccat gcgcccttct ctgctcacgc ttcgtgttgg ttttgctctg
     1081 ccggcgctcg cctgcgcggc actgctcggg ttcggttatt accttcagta cgtcgagggc
     1141 ctgaacccct gcccgctctg ccttgtgcag cgcggctttt tctatgccgt ggcggggacc
     1201 tgcgtgatcg cctcgattca cgcaccgcgg ggctggggca ggacggcgta cggactggtc
     1261 gccgcgcttt tcgcggccgg cggctttgcg accgccgccc ggcaggtgtg gctccagcac
     1321 ctgccgcctg acaaggtgcc gcagtgcggg cccgatctct atttcatgtt tgaaaacttc
     1381 ccgctgtcgc gcgttctgaa gacgctcatc acgggcaccg gtgaatgcgc ggtggtggac
     1441 tggactttcc tgggactctc gatcgccgag tggtcgctcg gctgtttcgc ggcgctgttc
     1501 gtctacgcgc tctggctcgc gctgcgcagg ccgaaggccg gtgcggccgc ggacgtgagt
     1561 tttcccgagg tcgaaggcaa gttccgctga gcttcgcgcg cggcgaacac ctcgccgatc
     1621 aggacgagcg cggccccgcc ttcgagcgcc gctgcaaggt ccggcaacgc ggcgagattc
     1681 ccgcgacgca tgctcgaaga gtcgaggctc acattctccg cgatcgcgac cggggtggtc
     1741 gcgctcatgc cttgccgcaa cagcgcctcc ttgacagcct cggcttcgcc cccggccatg
     1801 taaagcaccg cggtgtcggc ggctgccacc gccttttccc agcccgacgg ctcggcgcct
     1861 gccaccgtgc ggggcgtggc aaataccacg ctgcgcgaga ccagccgctg cgtgagcgac
     1921 acgccgagct cagcgctcgc cgcgagcgcg gccgttatgc cgggaacgac ctcgacgttg
     1981 atcgagttct ctttcaggaa gctgatctcc tcgtgcgcgc ggccgaagag catcgggtcg
     2041 ccacctttca gccgcactac cacgcggtgg cggcgcgcgg cgtgcgcaag gcgcttgttg
     2101 atgaagcgct gcgcggtcga atggcggccg cagcgctttc ccacggcgat cttctcggcg
     2161 cgcgctgcca gcccgagcgt ttcggggtga accagggcgt cgtagaacac gatgtctgcc
     2221 tccgccagca gccgcgccgc gcgcaccgta agcaggtcgg gcgcgcccgg ccccgcaccg
     2281 acgaggtaca ccgtgccaac ccaatcgggg tcagagcccg atttctttcc atggtgtcca
     2341 accccgacgg tcatggcctc accccggccc gatagccgcg ctggtaggaa tggctgaagt
     2401 cctcgaacga ggcgagcgcc tcgtgcgggt cttcgccgtc gcgcaactcg aacgcgtcga
     2461 agccgcagct ttcgaggaag aggagcaagt cgcgcgtaat gtgtccgacg gcgcgaagct
     2521 cgccgcggta gccgtagcgc tcgcgcaaaa gccgcgcgat cgagtagccg cggccgtcgc
     2581 cgaacttcgg gaagttcacc tccacgcggg cgacgccttc gagcgatatc gacgcgggat
     2641 cgtccgccgg ctccaggcgc agcacctttt cctcaggcca gggccggctc tcggctacga
     2701 cgcgccgccc cttgatgagc gtggccataa acgtgctcct tgaaaggttc gatgccgatg
     2761 cgatgcacca cgtcgacgaa gcgctcggct tcgctgtcgc ggtgctgcag gtagcagccg
     2821 atcagccgct cgataacgtc gggtacttcg gctcgcgcaa acgcggggcc gatgattttt
     2881 ccgagcgagg caggcgcctg atttcccccg acctcgatct ggtaccactc ttccccgttc
     2941 ttatcgacgc ccaggattcc gatatgaccg acgtggtggt ggccgcaggc gttgatgcaa
     3001 ccggagatgt tgaggtcgag ctcgccgatt tcgtgcaggt agtcgaggtc gtcaaagcgc
     3061 tcctggatcg cctgcgctac cgggatggac ttggcgttgg cgagcgcgca gtaatcgccg
     3121 cccgggcagc ttatgatgtt ggtgagaaga cctatgttgg gcgtcgcgag accgagctcg
     3181 cgcgtctcgc gccagagcgc cggcaggtcg cgccgccgga cgtcggcgag caccaagttc
     3241 tgctcgtggg acacgcgcag ctcgccgaac gagtagcgct cggcgagatt cgcgatcgcg
     3301 tccatctgct cgctcgtcac atcgcccggc ggaacgccgg tcttcttgag cgacaaggtc
     3361 accgccgcgt agccggggac tttgtgagcc tgcacatttc tcttcttcca cgcgacaaag
     3421 cctggctcgg aaaaatcggg ctctgacccc gattttcggt actggggtcg gctgaagcgc
     3481 tgttcgacgc gggcgatgtc ttcggcggtg agagtcgccg ggccgtcctt gacgtgcgcc
     3541 cattcggctt ccacttcgtc gcggaagcga tcggcgccac gctccttgac caggatcttg
     3601 atgcgcgcct tgtggatgtt gtcgcgccgc ccatggcggt tgtagacgcg caggatcgcc
     3661 tcgacgtagg tgaggagatg cggccagggc aggaattcgc ggatcgccac gccaatcatc
     3721 ggtgtgcgac ccagcccgcc tccgacgagc acgcggaagc cgatctgttc tccctttctt
     3781 attgcatgta aaccaatgtc atgaacgccg actgccgccc ggtcggcgcg tgcgccgttc
     3841 acggcgatct tgaacttgcg cggcaggaac gagaactcgg gatggaaggt cgaccattgc
     3901 cgcaggatct cgcaccacac gaacgaatcc acgatctcgt cgacggccac gcccgcgaag
     3961 tggtcggtgg tgacgttgcg aaacgagttg cccgaggtct ggat
//
LOCUS       DASTPD010000062         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_232723, whole genome shotgun sequence.
ACCESSION   DASTPD010000062 DASTPD010000000
VERSION     DASTPD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232723"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="hemB"
                     /locus_tag="VFR57_02430"
     CDS             complement(<1..792)
                     /gene="hemB"
                     /locus_tag="VFR57_02430"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0258261.1"
                     /translation="MNIYGSFPALRMRRMRRDEFSRRLMRETTLMAGDLIYPVFVLDG
                     KARTEKVASMPGVERMTVDRLLRLAEQCVKFGIPVLALFPAIDSGLKTADGREAFNPK
                     GLVPRAIAALKKRFPELGIMADVALDPYTTHGQDGVIDATGYILNDATLDILAKQALA
                     EAAAGVDIVAPSDMMDGRIGRIRAALEKAGHIHTKIMAYSAKYASGFYGPFRDAVGSA
                     KALGKADKRTYQLDPANSDEALREVGLDLAEGADMVMVKPGLPYLD"
     gene            complement(934..1548)
                     /gene="yihA"
                     /locus_tag="VFR57_02435"
     CDS             complement(934..1548)
                     /gene="yihA"
                     /locus_tag="VFR57_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU0258262.1"
                     /translation="MISARIEGRDFIRLFSQAQFVAAAGRPAEFPRPGPPEIVFAGRS
                     NVGKSSAINAIIGRKRLAFTSKTPGRTQTINFYSLGDSARLVDLPGYGYARVPRAQRV
                     RWRELVGSYLGSRSDLAGIVVVMDARHPLTPLDAQLLEWLGDRRMLLMLTKADKLSRG
                     EQAALKGRFADALLFSSVTKRGVEECRALLESWLEQGAEIKSPR"
     gene            1553..2173
                     /locus_tag="VFR57_02440"
     CDS             1553..2173
                     /locus_tag="VFR57_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0258263.1"
                     /translation="MIRGLACVAGATAALAAQAQAPDLKKAEELANQVCAACHAGDGN
                     STAPANPKIAGQIPEYLHKQLVDFKSQGNKKPARESAIMAGMVANLSEADMKGLAAFY
                     ARQKLNPAAAADKNLAALGQKIWRGGIAASGVPACAGCHGPAGAGMPAQYPRIAGQYA
                     EYIAAQLKAFKDGARANDPNGMMRGVTARMTEREIRAVAEYAAGLR"
     gene            2243..2701
                     /locus_tag="VFR57_02445"
     CDS             2243..2701
                     /locus_tag="VFR57_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0258264.1"
                     /translation="MKKTLLAAGVAIVVGGLAGAALAQVKPDVLVKQRQSAMTLIGKY
                     WGPIAGMASGKVSPYNADVVSRNATYLENLAQMPWDGFHESAKGEKSRALPAIWEQKA
                     KFDELAQRLQTETIKLGQVARAKDEAGVKQQYAAVGKVCGACHESFRENQ"
     gene            2709..>2965
                     /locus_tag="VFR57_02450"
     CDS             2709..>2965
                     /locus_tag="VFR57_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0258265.1"
                     /translation="MFQASPALAGLLLFFAVGLCFAQGDPKRGEYVAKAGGCLGCHTE
                     EKENATPYAGGRALRTPFGTFYGPNITPHPQAGIGRWTEAD"
BASE COUNT          538 a          925 c          987 g          515 t
ORIGIN      
        1 gtcgaggtac ggaaggcctg gcttcaccat caccatgtcg gcgccttcgg cgaggtcgag
       61 accgacctcg cgcaacgcct cgtcggagtt cgcggggtcg agctggtagg ttcgcttgtc
      121 ggctttgccg agcgccttcg ccgagccgac cgcgtcgcgg aacgggccat agaagccgga
      181 cgcgtacttc gccgagtacg ccatgatctt ggtgtggata tgaccggctt tttcgagcgc
      241 cgcgcggatc cggccgatgc ggccgtccat catgtcggaa ggcgcgacga tgtccacacc
      301 tgcagcagct tcggcgagcg cctgctttgc caggatatcg agcgtcgcgt cgttgaggat
      361 gtatccggtt gcgtcgataa cgccgtcctg accgtgcgtg gtgtacggat cgagcgccac
      421 gtcggccatg atgcccagtt cgggaaagcg cttctttagt gcggcgatag cgcgcggaac
      481 cagtcccttc ggattgaacg cttcgcggcc gtcggccgtc ttaagcccgg aatcgattgc
      541 ggggaacagc gcgagcaccg ggattccgaa tttcacgcat tgttcggcaa ggcgcagcag
      601 ccggtcgacg gtcatgcgct cgacgcccgg catggaagcc actttctccg tgcgagcttt
      661 tccatccagc acgaagaccg ggtagatgag atcccccgcc atcaatgtgg tctcgcgcat
      721 caggcggcgc gagaactcgt cgcggcgcat gcgccgcatc cgcagcgccg ggaagctacc
      781 gtaaatgttc atttatttcc aaatgttacc gggggaactt tgcggggcgg gctgaatctt
      841 accttcggac gagttgatcg tctcccgctt ctcctccctg agcgggtgcc ttaattcaac
      901 gttaaggcgt tttgcccccg gtttgtatcc cctttaccgg gggcttttta tttcggcacc
      961 ctgctcgagc caggactcga gcagggcgcg acattcctcg acgccgcgct tcgtcacgct
     1021 cgagaagagt agcgcatcgg cgaaccgtcc cttgagcgcc gcctgctcgc cgcggctaag
     1081 cttgtccgcc ttcgttagca tgagcagcat gcgtcggtcg ccgagccatt ccaagagctg
     1141 cgcgtcgagc ggcgtcagcg ggtggcgagc gtccatgacg accacaatgc cggccaggtc
     1201 agaccgcgag ccgagatagc ttccgacgag ctcgcgccac cgcacccgct gcgctcgcgg
     1261 gactcgcgca tacccatacc ccgggaggtc gacgagccgg gcggaatcgc ccagcgagta
     1321 gaagttgatc gtctgcgtgc gcccgggagt cttgctggta aacgcgaggc gcttgcgccc
     1381 gatgatggca ttgatcgcgc tcgacttccc gacgtttgag cggccggcga agacgatctc
     1441 cgggggcccg ggccgcggga actcggctgg ccggccggca gccgcgacga actgtgcttg
     1501 cgagaataat ctaataaaat cgcgtccttc gatgcgagct gagatcaagg ggatgatacg
     1561 tggattggcg tgcgtcgccg gcgcgacggc cgcgctcgcg gcgcaggctc aagcgcccga
     1621 tctgaaaaaa gccgaggagc tcgcgaacca ggtttgtgcc gcctgtcatg ccggcgacgg
     1681 caacagcacg gcgcccgcga atccgaagat cgccggtcag atcccagagt atctgcacaa
     1741 gcagctcgtg gacttcaagt cgcaggggaa caagaagccg gctcgcgaga gcgcgatcat
     1801 ggcgggtatg gtcgcgaatc tttccgaagc cgacatgaaa ggcctcgcgg cgttttacgc
     1861 ccgccaaaag ctgaacccgg ccgctgccgc ggacaagaat ctcgccgcgc tgggccagaa
     1921 aatctggcgc ggcggaattg ccgccagcgg cgtgcctgcg tgcgctggat gccatggccc
     1981 cgccggcgcc ggaatgcccg ctcagtaccc gcgcattgcc ggccagtacg ccgagtacat
     2041 tgccgcccag ctcaaggctt tcaaggacgg cgcccgggcg aacgacccca acggcatgat
     2101 gcgcggcgtg actgcgcgca tgaccgagcg ggagattcgc gccgtggccg agtacgccgc
     2161 agggctgcgc tgagggaata gttgcttcgg ccgtagtgtt ttcacccgtg cggctcatgc
     2221 cgcgattaga ggagaggaaa cgatgaagaa gacccttctg gcagccggcg tcgcgatcgt
     2281 cgtcggcggc ctcgccggcg ccgcgcttgc ccaggtgaag cccgacgtac tggtgaagca
     2341 gcggcaatcg gcaatgacgc tgatcggcaa gtactggggc ccgatcgccg gcatggcttc
     2401 gggcaaggtg tctccttaca acgcggacgt tgtatcgcgc aacgctacgt acctcgaaaa
     2461 cctcgcgcag atgccgtggg acgggttcca cgaaagcgcc aagggcgaga agagccgcgc
     2521 gctgcctgcg atatgggagc aaaaggccaa gttcgacgag ctcgcgcaaa ggttgcagac
     2581 ggaaaccatc aagctcggcc aggtggcgcg cgcgaaagac gaggcgggcg tgaagcagca
     2641 atacgccgcg gtcggcaagg tatgcggcgc ctgccacgag agtttccgcg agaatcagta
     2701 ggtagccgat gtttcaggca agccccgcgc tcgcggggct tttgcttttc ttcgccgtcg
     2761 ggctgtgctt cgcgcagggc gatccaaagc gcggcgaata cgttgcgaag gcgggcggct
     2821 gcctcggctg tcataccgag gagaaggaaa acgcgacgcc ttatgccggc ggccgcgcgc
     2881 tgaggacgcc tttcggcacg ttttacgggc cgaacatcac gccgcatccc caggcaggga
     2941 tcgggcgctg gaccgaggcc gattt
//
LOCUS       DASTPD010000063         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1661122, whole genome shotgun sequence.
ACCESSION   DASTPD010000063 DASTPD010000000
VERSION     DASTPD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661122"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFR57_02455"
     CDS             complement(<1..842)
                     /locus_tag="VFR57_02455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0258266.1"
                     /translation="MELALQVGKYDVQRLLGKGACGTVYLARDTFTGKEVALKTIEPE
                     VLLDPQFGRVYRSQFLNEASLAGKLRHPHIVSIYDAVVDEDSGHIAMELVTGGDLTKY
                     GSSGTLLPVADVLQIGFKCCGALDYAAKEGIVHRDIKPANIMLAGGTEVKIADFGAAS
                     LKMTQAVQTAALGSPYYMSPEQIEGGKPVTFHSDIYSLGVVLYELLTGKRPFVGANLQ
                     ELMNRILKFEPLPPSEARPELPKSLDAVVLRALKKDPAQRYASWTDFALELSRAGELV
                     LPPGA"
     gene            complement(842..2278)
                     /locus_tag="VFR57_02460"
     CDS             complement(842..2278)
                     /locus_tag="VFR57_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0258267.1"
                     /translation="MGVHQNFINGEWALTREQAPNINPSNIADVVGDFARADAAQARA
                     AIAAARAAFPAWSRGPVQARADMLDKIGNEILARKDELGTLLAREEGKTRPEALGEAT
                     RAGYLFKFFAGEVVRQAGEVLPSVRPGIKVEITREPVGVVGIITPWNFPLAIPAWKIA
                     PALAYGNCVVFKPADLVPGCAWALTEIIAKAGVPAGVFNLVMGRGSVLGELLSTDPGI
                     DAVSFTGSQAVGKRVAHNCAARLAKVQLEMGGKNPQVVLDDADLDTAVNLSIQSAFFS
                     TGQRCTASSRLIVTEGIHDKFINGVTNKLKALKVDDALKEGTDIGPVVDERQLEQDLR
                     YIELGRKEGARLAAGGGRLEREKQGYYLEPALFVDTTSSMTINREEIFGPVAAVIRVK
                     NYDEALAVANDTPFGLSSGIATTSLKYAEHFKRNSAAGMVMVNLPTAGVDYHVPFGGR
                     KGSSYGPREQGRYAVEFYTTVKTSYINP"
     gene            2288..>2599
                     /locus_tag="VFR57_02465"
     CDS             2288..>2599
                     /locus_tag="VFR57_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258268.1"
                     /translation="MIFDPALIQRGARLKRSPYFEATQRAGCRGYTVYNHMLLPIRYD
                     SFEAEYQRLLADVTLWDVSVERNVEIAGPDGVRFAELLTPRDLSKCAVGEGRYVILTD
                     EA"
BASE COUNT          429 a          879 c          824 g          467 t
ORIGIN      
        1 gcgcccggcg gcagcacgag ctcgcctgcc ctggagagct cgagcgcaaa atcggtccag
       61 ctcgcgtagc gctgcgccgg gtctttcttg agagcgcgta gcaccacagc gtcgagcgac
      121 ttcggcagct cggggcgcgc ctcgctcgga ggcagcggct cgaacttgag gatgcggttc
      181 attagctcct ggaggttcgc gccgacgaac gggcgcttgc cggtaagcag ctcgtacagc
      241 accacgccga gcgaatagat gtcgctatgg aaggtgaccg gcttgccgcc ttcgatctgc
      301 tccggcgaca tgtaatacgg cgagccgagc gccgcagtct gcaccgcctg ggtcatcttc
      361 agggacgcgg cgccgaagtc ggcaattttc acctcggtgc ccccggcgag catgatgttc
      421 gccggcttga tgtcgcggtg gacaatgccc tccttcgccg cgtagtcgag cgcgccgcag
      481 cacttgaagc cgatctgcag cacgtccgcg accggcaaca gcgtcccgga cgagccatac
      541 ttggtgaggt cgccgccggt cacgagctcc atcgcgatgt ggcccgagtc ttcgtccacc
      601 accgcgtcgt agatgctgac gatatgcgga tggcgcagct tccccgcgag cgaagcctcg
      661 ttcaggaact gcgagcggta caccctgccg aactgcggat cgagaagtac ctcgggctcg
      721 atcgtcttga gcgccacctc cttgccggtg aaggtgtccc tggcgagata gaccgttccg
      781 cacgctccct tgccgagaag ccgctgcaca tcgtacttcc cgacctggag ggccagctcc
      841 attacgggtt tatgtagctc gtcttgaccg tggtgtagaa ctcgaccgcg tatcgcccct
      901 gctcgcgcgg tccgtagctc gagcctttgc gtccgccgaa cggaacgtgg tagtcgaccc
      961 ctgcagtcgg caggttgacc atcaccatgc cggcggcgga gttgcgcttg aagtgctcgg
     1021 cgtacttgag cgaagtcgtg gcgatgccgg aagagaggcc gaacggtgtg tcgttcgcca
     1081 ccgcgagcgc ctcatcgtag ttcttgacgc ggatcaccgc cgcgaccggg ccaaagatct
     1141 cctcccggtt gatcgtcatc gaggaggtgg tgtcgacgaa gagcgccggc tcgaggtagt
     1201 agccctgctt ttcgcgctcg agacggccgc caccggcggc gagcctcgcg ccctccttgc
     1261 gaccaagctc gatgtagcgc agatcctgct cgagctgtcg ctcgtccacc accgggccga
     1321 tatcggttcc ttccttcaga gcgtcgtcga ctttgagggc tttcaacttg tttgttaccc
     1381 cgttgataaa tttgtcgtgg atcccttcgg tcacgatcaa gcggctcgag gcggtgcagc
     1441 gctgcccggt ggagaaaaat gcgctctgga tggaaagatt caccgcggtg tcgaggtcgg
     1501 catcgtccag caccacctgc ggattcttgc cgcccatctc gagctgcacc ttggcaagcc
     1561 gcgcggcgca attgtgcgcg acgcgcttgc cgacggcctg cgagccggtg aagctcaccg
     1621 cgtcgatgcc cggatcggtg gaaagcagct cgcccagcac cgagccgcgg cccatcacca
     1681 ggttgaacac gcccgccggc acacccgcct ttgcgatgat ctcggtaagc gcccaagcgc
     1741 agcccggcac gaggtccgcc ggcttgaaca ccacgcagtt gccgtaggcc agcgccgggg
     1801 cgatcttcca ggccgggatc gcgagcggga aattccacgg cgtgatgatc ccgaccacgc
     1861 cgaccggctc gcgcgtgatc tcgaccttta ttccgggtcg caccgagggc agcacttcgc
     1921 ccgcctggcg caccacctcg cccgcgaaga acttgaagag atagcccgcg cgcgtcgctt
     1981 cgccgagcgc ctcgggcctg gtcttgccct cctcgcgggc aagcagcgtg cccagctcgt
     2041 cctttcgcgc caggatctcg ttgccgattt tgtccagcat gtcggcgcgc gcctgcaccg
     2101 ggccgcgcga ccaggccgga aaagcggcgc gcgcggcagc gatcgcggcc cgggcctgcg
     2161 cggcgtcggc gcgcgcgaaa tcgccgacca cgtcggcgat gttcgacggg ttaatatttg
     2221 gcgcctgctc gcgcgtcagc gcccattccc cattgatgaa attctggtgt actcccatgg
     2281 aaggccaatg atattcgatc ccgccctgat ccagcgtgga gcgcgtctca agcgctcgcc
     2341 ctacttcgag gcaacgcagc gcgccggctg ccgcggctac accgtgtaca accacatgct
     2401 gctgccgatc cgctacgaca gcttcgaggc ggagtaccag cggctgctcg ccgacgtgac
     2461 gctctgggac gtatcggtcg agcgcaacgt ggagatcgcc ggacccgacg gcgtcaggtt
     2521 cgccgagctg ctcacgccgc gcgacttatc caaatgtgcg gtcggcgaag gacgctacgt
     2581 cattctcacc gacgaagcc
//
LOCUS       DASTPD010000064         6319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_641444, whole genome shotgun sequence.
ACCESSION   DASTPD010000064 DASTPD010000000
VERSION     DASTPD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641444"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFR57_02470"
     CDS             complement(<1..569)
                     /locus_tag="VFR57_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258269.1"
                     /translation="MLVVSALLFALGYWTYSQVERSLREIRASTLQSLLDSGVNALRV
                     WINEEIANAQRLARDARVREGIGALARNPGCSGSARAHLEEVMRPLLREGGDATFNVV
                     ARGGQLLATRFPAYCGRDVSAAFLAELEPVFEGKARFIRPFRDAERIGTPPERVRPGP
                     PFAWVYAPVRDGGKVIAALGIAQPVDAVFG"
     gene            complement(644..1591)
                     /locus_tag="VFR57_02475"
     CDS             complement(644..1591)
                     /locus_tag="VFR57_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0258270.1"
                     /translation="MLLKAEFQQPAVAASPIDRVVGAASGIILGKETQVRLALGCLLA
                     RGHLLIEDLPGVGKTTLAHVLAKALGLHFQRIQFTSDMLPADILGVSIYERESGAFKF
                     HPGPIFAQVILADEVNRATPKTQSALLEAMEEHQVTAEGETRKLPEPFFVIATQNPSD
                     QVGTFPLPESQLDRFMMRIELGYPDRDAERALLSGTDRRDLLATLDPCMTPAELMELQ
                     SSVQRMYVSPALLDYVQAIVEHTRRSPEYLTGLSPRAALALVHSAKAWALLEGRDKVI
                     PEDVQAIVPGVAAHRLRPAHDAGRRVDVGAQLLAAVPIP"
     gene            1630..2556
                     /locus_tag="VFR57_02480"
     CDS             1630..2556
                     /locus_tag="VFR57_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEU0258271.1"
                     /translation="MPAAFVTHPECFKHEMGAHHPERPARLSAIEDQLIASGLGQYLA
                     RYDAPLASDEQLARVHPPEYVQAIREFAPSSGTVHLDPDTAMNPFSLQAALRAAGAAV
                     LATDLVLTRKHNSAFCAVRPPGHHACRARPMGFCIFNNVAVAARHALAAHGLERVAII
                     DFDVHHGNGTEDIFEGDEQVLMASIFQHPFYPYSGTESPASNMHNIPLAAGSGSKELK
                     EVVLEEWLPALNDFRPELVLFSAGFDAHIEDDMAMLRFTDGDYAWVTEQVKLVAERYA
                     KGRMVSMLEGGYALSALGRSAVQHIRVLAGLN"
     gene            complement(2714..3700)
                     /locus_tag="VFR57_02485"
     CDS             complement(2714..3700)
                     /locus_tag="VFR57_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258272.1"
                     /translation="MIRNAPLQLLALAWALAAAGAALAQPVQKVPRIGMLFAPPEATH
                     APLRDAFLQGLRDLGYEEGRTIVLEKRYAEGHPERLPKLASELVELKVDVIVTAAFPA
                     INAARQATTTIPIVFAATGDPVAAGHISSLARPGGNITGLSVLATETTPKRLQLLKDV
                     HPGAARVALLFNPTDFGMTVRVTEAQSAARALGIATTTHEVRNSADFDRAFAAMEKAR
                     PDALLAVVDGLTLQHRRRIVDFAAANRIAAIYETSEFVESGGLMSYGPSAADNYRRAA
                     NYVDRILKGAKPAELPVEQPSKIELVINAKSARALGLVIPQSVLLRADRILD"
     gene            complement(3720..4127)
                     /locus_tag="VFR57_02490"
     CDS             complement(3720..4127)
                     /locus_tag="VFR57_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEU0258273.1"
                     /translation="MSPPRPVLLFDGVCKLCTGSVAFVLRHEEAQDLRFAPLQSPAGM
                     RLMQEFGIDPTQMKTFVVIADGRAYVRSDAAIRVARFLRGAWKLLGVVRIVPRSIRDY
                     AYDVVARNRYRWFGRHDACIAPTPELRKRFIER"
     gene            complement(4114..5139)
                     /gene="mnmH"
                     /locus_tag="VFR57_02495"
     CDS             complement(4114..5139)
                     /gene="mnmH"
                     /locus_tag="VFR57_02495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEU0258274.1"
                     /translation="MNFKRFDAIIDVRSPAEYAEDHIPGAISAPVLDDAERAKVGTLY
                     KQVSAFSAKKAGASLISRNIARHIEELFRENDKTWHPLVYCWRGGKRSAAMAHVLREI
                     GWNAETLEGGYKAYRRFVVSQLQSLPPGLEFIVIHGPTGSGKSRLLGALKRAGAQVLD
                     LEGLAAHRGSVLGNLPGRPQPTQKMFESLLLQQLSGPRPEKPVYVEGESKKIGELHVP
                     EALMERMRASPCIVLDTSIDMRVDLLLDEYRHFLSDRDALNKQLDCLVALHGRERIAE
                     WKSITDWRELVRRLLVEHYDPAYRRSSNRNFIQLADAAPVSIARADDDAFAAAARSIL
                     DLTPVTA"
     gene            5158..6201
                     /gene="selD"
                     /locus_tag="VFR57_02500"
     CDS             5158..6201
                     /gene="selD"
                     /locus_tag="VFR57_02500"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU0258275.1"
                     /translation="MNAPERIRLTEFSHGGGCGCKIAPAVLSEILAAAPKGSVFSELL
                     VGTETADDAAVYKLNETQALVATTDFFTPVVDDPYDFGRIAATNAISDVYAMGARPIM
                     ALALVGMPLEKLPLSVIRRVLEGGESVCAAAGIPIAGGHSIDTLEPIYGLVALGLVHP
                     ERVKRNSSGKPGDVLILGKPLGIGILSAALKKGKLSAAGYAQMVEWTTRLNTPGEALA
                     AMPQVHALTDVTGFGLAGHLLEVCRGSRLSAQVVFSSIPILGEALDWVKQGVATGASD
                     RNWKGYGHEVEFSGEDWKRKLLTDPQTSGGLLVACAPDVQERVLDIFRKQGFGEARAI
                     GRLVPGPPKLTVR"
BASE COUNT         1081 a         2029 c         2160 g         1049 t
ORIGIN      
        1 ccgaacacgg cgtcgaccgg ctgggcgatc ccgagggccg cgatcacttt cccgccgtcg
       61 cgcaccggcg catacaccca ggcgaacggc ggtccgggcc gcacgcgctc cgggggcgtc
      121 ccgatgcgct cggcgtcccg gaagggccgg atgaagcgcg ctttgccttc gaataccggc
      181 tccagctcgg caagaaacgc cgcgctcacg tcgcggccgc agtacgccgg gaaccgcgtc
      241 gcgaggagct gcccgccgcg cgccaccacg ttgaaggtcg cgtcgccgcc ttcgcgcagc
      301 agcggccgca tgacttcctc caagtgcgcg cgcgcgctgc ccgagcagcc cggattgcgg
      361 gcgagggcgc cgatcccttc gcgcacgcgc gcatcgcgcg cgaggcgctg ggcgttggcg
      421 atctcctcgt ttatccagac gcgcagcgca ttgacgccgg agtcgagcag gctttgcagc
      481 gtgctcgcgc ggatctcgcg cagcgagcgc tccacctggc tgtaggtcca gtagccgagc
      541 gcgaaaagaa gcgcggagac cacgagcacg ccggtagccc aagccggaac ggaaaccagc
      601 cgttgttgcg gctgccccga ggccggatga agagtgtcct cggctatggt atgggaacag
      661 cggcgagcag ctgcgcgccg acgtcgacgc ggcgtccggc gtcgtgcgcc gggcgcaggc
      721 gatgcgccgc gacgccgggc acgatggcct gaacgtcttc ggggatcacc ttgtcgcggc
      781 cctcgagcag cgcccatgcc ttcgccgaat gcaccagcgc gagcgcggcg cgcggggaga
      841 ggcccgtgag gtactccggg gagcggcgcg tgtgctcaac gatggcctgc acgtagtcga
      901 gcagcgcggg cgagacgtac atcctctgca cgctggactg cagctccatc agctccgcgg
      961 gcgtcatgca tggatcgagc gtcgcgagca gatcacgccg gtcggtgccc gaaaggagcg
     1021 cgcgctcggc gtcgcggtcg ggatagccga gctcgatgcg catcatgaag cggtcgagct
     1081 gcgactcggg caacggaaac gtgcccacct ggtcggaggg gttctgagtg gcgatgacga
     1141 agaagggctc gggcagcttg cgcgtctctc cctcggccgt gacctggtgc tcctccatcg
     1201 cttcgagcag cgccgactgc gtcttcggcg tggcgcggtt gacttcgtcg gcgaggatga
     1261 cctgcgcgaa gatcggcccg gggtggaact tgaaggcgcc cgactcgcgc tcgtagatcg
     1321 acacgccgag gatgtcggcg ggcagcatgt cgcttgtgaa ctggatgcgc tggaaatgca
     1381 agccgagcgc cttggccagc acgtgggcga gggtggtctt gccgacaccg ggaaggtcct
     1441 cgatcagcag gtggccgcgc gcgagcaggc acccgagcgc gagacgcacc tgggtttcct
     1501 tgccgaggat aatgccgctc gcggcgccca ctacacgatc gataggcgac gccgcgacag
     1561 cgggctgctg gaactcggcc ttgagaagca tgcggcgatt gtactcgatg cgataagatg
     1621 gaaattcgca tgcctgccgc atttgtcaca catccagagt gcttcaagca cgaaatgggc
     1681 gcgcatcatc ccgagcgccc ggcgcgcctc tccgcgatcg aggaccagct catcgcttcc
     1741 ggcctgggcc agtacctcgc gcgctacgac gcgccgctcg cgagcgacga gcagctggcg
     1801 cgcgtgcacc cgcccgagta tgtgcaggcg atccgcgagt tcgcgccgag cagcggcacc
     1861 gtgcatctgg acccggacac cgcgatgaac ccgttcagcc tgcaggcggc gctgcgcgcc
     1921 gcgggcgcgg cggttctcgc gaccgacctc gttctcacaa gaaagcacaa ttcggcgttc
     1981 tgcgccgtcc gcccgccagg ccatcacgcc tgccgcgcgc ggccgatggg gttctgcatc
     2041 ttcaacaacg tcgcggtcgc cgcgcgccac gcgctcgctg cgcatggact ggagcgcgtg
     2101 gcgatcatcg acttcgacgt gcaccacggc aacggcaccg aggacatctt cgagggcgac
     2161 gagcaggtgc tgatggcgtc gatcttccag cacccgttct atccgtacag cggcaccgaa
     2221 agtccggcgt ccaacatgca caacatcccg ctcgccgccg ggtcgggctc caaggaactg
     2281 aaagaggtgg tgctcgagga atggcttccg gcgctgaacg acttcaggcc cgagctcgtt
     2341 cttttctccg ccggattcga tgcgcacatc gaggacgaca tggcgatgct gcgcttcacc
     2401 gacggcgact acgcctgggt caccgagcag gtgaagctcg tggccgagcg ctacgcgaag
     2461 ggccgcatgg tgtcgatgct cgagggcggc tatgcgctct ccgcgctggg ccgcagcgcc
     2521 gtgcagcaca tcagggtcct cgcgggactg aactaggtct caagaatcct gcagtcaacg
     2581 gtcagttctt ctccggctgc gatctcgcgt accgccacgt cgacgccatg gcgctcgcgc
     2641 gcgaagaccg gacgaaggat ttggcgtatc tgcgctcgag cctgaccggc acgaggagcg
     2701 ccacgcgcaa ggatcagtcg agaatccgat ccgcgcgcag caggaccgac tgggggatga
     2761 cgaggcccaa cgcccgcgcg ctctttgcgt tgatcaccag ctcgatcttc gagggctgct
     2821 cgaccggaag ttcggcgggc ttcgcgcctt tcaatattcg gtccacatag ttggctgccc
     2881 gccggtagtt gtccgcggcc gaaggtccgt aggacatcag gccgccggac tcgacgaact
     2941 cgctggtctc gtagatcgct gcgatgcggt tcgcggcggc gaagtccacg atgcgcctgc
     3001 ggtgctgcag tgtgaggccg tccaccaccg cgagcagggc atcgggccgc gccttttcca
     3061 tggcggcgaa cgcccggtca aagtcggcgc tgttgcgcac ttcgtgcgtc gtcgtcgcga
     3121 tgccgagcgc gcgcgcggcg gactgcgctt cggtgacgcg caccgtcatg ccaaaatcag
     3181 tgggattgaa aagcagcgcc acgcgggcgg cgcccggatg gacgtccttg agcagctgga
     3241 gccgcttcgg ggtcgtctcg gtcgcgagca ccgacaatcc ggtgatgttg ccgcctgggc
     3301 gcgccaggct cgagatgtgc ccggcggcga ccggatcgcc cgtggctgcg aacacaatag
     3361 ggatcgtggt ggtcgcctgc cgcgcggcgt tgatggccgg gaacgccgcc gtcacgatga
     3421 catcgacttt cagctcgacg agctccgacg cgagcttcgg caggcgctcg ggatgtccct
     3481 cggcgtagcg cttttcgagc acgatcgtgc ggccttcttc gtacccgagg tcgcgcagcc
     3541 cttggaggaa tgcgtcgcga aggggcgcat gcgtcgcctc cggcggtgcg aacagcatcc
     3601 cgatgcgcgg aaccttctgc accggctggg caagggctgc tccagccgcc gcgagagccc
     3661 acgcaagcgc gagaagctgc agaggtgcgt tcctgatcat gcgtagccga gcaccgccgt
     3721 catctttcga tgaaacgctt ccgcagttcc ggcgttggcg cgatgcaggc atcgtggcgc
     3781 ccgaaccagc gataccggtt gcgtgcgacg acgtcgtacg catagtcgcg aatcgaccgg
     3841 gggacgatcc tgaccacgcc cagcagcttc caggcaccgc gcaggaagcg tgcgacgcgg
     3901 atcgccgcgt cggatctcac gtaggctctt ccgtcggcga tgacgacgaa cgttttcatc
     3961 tgagtgggat cgatgccgaa ctcctgcatc aggcgcatgc ctgccggcga ttgcagcggc
     4021 gcaaatcgca ggtcctgagc ttcttcgtgg cgcagaacga aagcaaccga tccggtgcag
     4081 agcttgcata caccatcaaa gagtagaacc ggcctaggcg gtgacagggg tcaggtcgag
     4141 gatggaccgc gccgcggcgg cgaaggcgtc gtcgtcggcg cgggcgatgg ataccggcgc
     4201 cgcgtcggca agttggatga agttgcggtt ggaggagcga cggtaggcag gatcgtagtg
     4261 ctcgacgagc agccggcgca ccagctcgcg ccagtcagtg atcgacttcc actcggcgat
     4321 gcgctcgcgc ccgtgcagtg cgacgagaca atcaagctgc ttgttcagtg cgtctctgtc
     4381 ggaaaggaaa tgccggtatt cgtccagcag cagatccacc cgcatatcga tgctcgtatc
     4441 gagaacgatg caaggcgatg cgcgcatgcg ctccatgagc gcttccggaa cgtgaagctc
     4501 gccgatcttt ttcgattcgc cttcaacgta caccggcttt tcgggccgag gacccgaaag
     4561 ctgctgaagc agcaggctct cgaacatttt ctgcgtgggc tgcgggcggc cgggcaggtt
     4621 gccaagcacc gagccgcggt gcgcggcaag gccctcgagg tcgaggacct gcgcgccggc
     4681 gcgcttgagc gcaccgagca gtcggctctt gccgctgccc gtagggccgt gaatcacgat
     4741 gaattccagc ccgggaggaa gagattgcaa ttgtgaaacc acgaacctgc gataagcctt
     4801 gtagccgccc tcaagggtct cggcgttcca gccgatctcg cgcaggacat gcgccatcgc
     4861 ggccgagcgc ttgccgccgc gccagcagta gacgagcgga tgccaggtct tgtcgttctc
     4921 ccggaagagt tcctctatat ggcgcgcgat gttgcgggag atgagtgaag ccccggcctt
     4981 cttggcggag aaagctgaga cctgcttgta cagggttccg accttggcgc gctcggcgtc
     5041 gtcgagcacc ggcgccgaga tcgcgccggg aatgtggtct tcggcgtact cggcgggcga
     5101 acgcacgtcg atgatggcgt cgaagcgctt gaagttcatg gttaaaattc tacgcttatg
     5161 aatgcacccg aacggatcag gctgaccgag ttcagccacg gcggcggctg cggctgcaag
     5221 atcgcgcccg cggtgttgtc cgaaattctt gcagcagcac caaagggaag tgtcttctcc
     5281 gagttgctgg tgggaactga aaccgcggac gacgcggcgg tctacaagtt gaacgaaacg
     5341 caggcactcg tggcgacgac cgacttcttt acgccggtgg tcgacgatcc gtacgacttc
     5401 ggccgcatcg ccgcgaccaa tgcgatctcg gacgtgtacg cgatgggcgc gcggccgatc
     5461 atggcgctcg cgctggtcgg catgccgctc gagaagctcc cgctgagcgt gatccggagg
     5521 gtgctcgagg gcggcgaatc ggtgtgcgcg gccgccggaa ttccgatcgc gggcgggcat
     5581 tccatcgaca cgctcgagcc gatctacggt ctggtggcgc tcggacttgt gcatccggag
     5641 cgggtcaaga gaaacagttc cggaaagccc ggcgatgttc tgattctcgg caagccgctc
     5701 ggcatcggca tcctctccgc cgcgctcaag aaaggaaagc tctcggccgc gggctatgcg
     5761 cagatggtcg agtggaccac caggctcaac acgccgggcg aagcgctcgc cgccatgccg
     5821 caggtacacg cgctcaccga cgtcaccggc ttcgggctcg cgggtcacct gctcgaggtc
     5881 tgccgcgggt cgcgcctctc cgcacaagtg gttttttctt ccattccaat cctcggagag
     5941 gcgctcgact gggtgaagca gggcgtcgct accggcgcct ccgaccgcaa ctggaaggga
     6001 tacgggcacg aggtcgagtt ctccggcgaa gactggaagc gcaagctcct gaccgatccg
     6061 cagacgagcg gtgggcttct cgtcgcctgc gcaccggacg ttcaggaaag ggtattggac
     6121 attttcagga agcagggctt tggggaggcg cgggcgatcg gccgccttgt tcccggtcca
     6181 ccgaagctga ccgtgcgctg aaatcggggt ctgggtgatc tcaacccgtc gaggcaacgg
     6241 ttgctcgtaa tgcctccaac ggcgtaagcc agtttagcgt ttcccgtggg cgctcgttaa
     6301 gcagacgcgc gacgcggtt
//
LOCUS       DASTPD010000065         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_233236, whole genome shotgun sequence.
ACCESSION   DASTPD010000065 DASTPD010000000
VERSION     DASTPD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233236"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFR57_02505"
     CDS             <1..333
                     /locus_tag="VFR57_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258276.1"
                     /translation="AFRVSQHRVKDGFLFDTQYPERTIYSESLEMRLTLAGQGGAKAP
                     AVEYRWLSDATDLADPRSWTKAELKDKVFRLKLRETETLSAELCLHAGKWPDGTTSEN
                     AGVDAALA"
     gene            complement(330..1772)
                     /locus_tag="VFR57_02510"
     CDS             complement(330..1772)
                     /locus_tag="VFR57_02510"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEU0258277.1"
                     /translation="MSGNPLDLRPWLAEVEKLGELKHVDGADWNLELGAISELNVKKD
                     APPALLFDQIKGYPKGFRVLTCSTSSPVRLSSILRLPIQRNHSGLVAVLRGKPAQWQA
                     AAKDFPFEVVTSGPVLENKQEGADVDVNAFPSPLWHELDGGRYIGTGCSVVTRDLDSD
                     WVNVGTYRVQVLDGKHVALDMVPGKHGRIQYEKYKRAGQRFPVAIVVGGDPLGYLISG
                     IEIPFGMCEWNYVGAILNQPVSAVLGKLTGLPFPAASEIVLEGFVEPNDERAEGPFGE
                     FHGYYPGKAGTAPAVTVERIYFRGEPIIVGSPPAKPPNDYSYSKAVMRSALLHDALVA
                     AGLSEVEAVWAHEIGGARMFNVVAIKQRYAGHARQAGHLLSQCGVGAYMSRYSVVVDD
                     DIDPSNLQEVMWAVATRTDPAVDIDIIHRGVGSKNDPMFVAYPYDAPLSSKAVIDACR
                     PWDHLDEFPIVAEASKELQDKTRAKWKDLL"
     gene            1800..2750
                     /locus_tag="VFR57_02515"
     CDS             1800..2750
                     /locus_tag="VFR57_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258278.1"
                     /translation="MKLLLVLCLSFVSFSAAPQSYPSRPVRVVVGFPPGGGTDILARV
                     LSPRLSEYLGQPFVVENRPGATTNIASDLVAKSAPDGHTLLFTTSALAINMSLYKNLP
                     FDAMRDFAPISVFAQSPNLLVAHSSAGASVKELLAQAKSKPGSMNYSSAGSGTSQHLA
                     GELFKARTGTYIVHIPYKGTAASLTAVIAGEVHFSFANVPAILGHVRSGRLKALAVLA
                     PKRSDLMPDVPTMEEAGVAGVEVPVWYALLGPAALPRDIVKTLNDATVRAARSPDLSQ
                     KLVEQGAEPVGNSPEEFSKLLREEVAKWAAVVKVSGAKAE"
     gene            2752..>2928
                     /locus_tag="VFR57_02520"
     CDS             2752..>2928
                     /locus_tag="VFR57_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258279.1"
                     /translation="MQKTPAALVVAENRAGAAGSIGAVAVAKSVPDGYTLLLGSSGTV
                     TAGPAVWKNLAFDPL"
BASE COUNT          503 a         1003 c          918 g          504 t
ORIGIN      
        1 gcgttccgag tctcgcagca ccgcgtgaag gacggctttc tcttcgacac gcagtacccc
       61 gagcgcacga tctacagcga aagcctcgag atgcgcctta cgctcgccgg ccagggcggc
      121 gccaaggcgc cggcggtgga gtaccgctgg ctatccgatg ccaccgatct cgcggacccc
      181 aggagctgga ccaaggcgga gctgaaggac aaggtgttcc gcctcaagct gcgtgagacg
      241 gagacgctct ctgcggagct ttgcctccac gccggcaagt ggcccgacgg gacgaccagc
      301 gagaacgcgg gtgtcgacgc cgcgctcgcc tagaggaggt ccttccattt tgcgcgcgtc
      361 ttgtcctgca gctccttgga cgcctcggcg acgatcggga attcgtcgag atgatcccac
      421 gggcggcacg cgtcgattac ggccttggag ctaagcggcg cgtcgtacgg ataggcgacg
      481 aacatcggat cgttctttga tcccacgccg cggtggatga tgtcgatgtc caccgccgga
      541 tcggtgcgcg tggcgacggc ccacatcacc tcctgcagat tcgagggatc gatgtcatcg
      601 tcgaccacca ccgagtagcg cgacatgtac gcgccgaccc cgcactggct gaggaggtga
      661 ccggcctggc gcgcatgccc tgcgtagcgc tgcttgatcg ccaccacgtt gaacatgcgt
      721 gcgccgccga tctcgtgcgc ccacaccgcc tcgacctcgg aaagtcccgc cgcaacaagt
      781 gcgtcgtgca aaagcgccga gcgcatcacc gccttggaat atgaatagtc attcggcggc
      841 ttcgctggcg gactgccgac gatgatcggt tctccccgaa agtagattct ttccactgtt
      901 accgctggcg ccgtcccggc cttgcccggg tagtagccgt ggaactcgcc gaacggccct
      961 tccgcgcgct cgtcgttcgg ctcgacgaag ccttcgagca cgatctccga cgcggcgggg
     1021 aaaggcagac cggtcagttt gccgaggacc gccgagaccg gctgattcag gatcgccccg
     1081 acgtagttcc actcgcacat gccgaacggg atctcgatcc cggagatgag atacccgagc
     1141 ggatcgccgc cgacgacaat cgccaccggg aagcgctggc ccgccctctt gtacttttca
     1201 tactggattc ggccatgctt gcccggcacc atatccaggg cgacatgctt tccatccaat
     1261 acctgaaccc tgtacgtgcc gacgttcacc cagtcggagt cgagatcgcg cgtcaccact
     1321 gagcagccgg tgccgatgta gcgcccgccg tcgagctcgt gccagagcgg ggaggggaaa
     1381 gcgttcacgt cgacgtcagc gccctcctgc ttgttttcca gcaccgggcc ggatgtcacc
     1441 acttcgaaag gaaaatcctt cgccgcggcc tgccactgcg ccggcttacc gcgtagtacg
     1501 gcgaccaatc cggaatgatt tctttgaatc ggtaacctga ggatcgacga caggcgcacc
     1561 gggctgctcg tgctgcaggt cagcacgcga aagcccttcg ggtagccctt gatctggtcg
     1621 aagagcagcg ccggcggcgc gtccttcttg acgttcagct cgctgatcgc gccgagctcg
     1681 aggttccagt ccgcgccgtc gacgtgcttg agctcaccga gcttttcgac ttcagcgagc
     1741 cacggccgca gatcgagagg attcccgctc atcgcggcgc attataagat tcgggggcca
     1801 tgaagcttct gcttgtgctc tgcctttcat tcgtttcgtt ctcggcggca ccgcagagct
     1861 atcccagccg cccggtgcgc gttgtcgtag ggttcccgcc cggcggcggc accgacatcc
     1921 tcgcgcgcgt gctctcgccg cgcctgtcgg agtatctggg ccagccgttc gtggtggaaa
     1981 accggcccgg cgcgaccacc aacatcgcga gcgacctcgt ggcaaagagc gcgcccgacg
     2041 gacatacgct gctcttcacc acctcggcgc ttgcgatcaa catgtcgctg tacaaaaacc
     2101 tccccttcga cgccatgcgc gatttcgcgc cgatctcggt gttcgctcag agcccgaacc
     2161 tcctcgtagc gcactcgagc gccggcgcga gcgtgaagga actgctcgcg caggcgaagt
     2221 ccaagccggg ctcgatgaac tactcttccg ccggctcggg cacgagccag catcttgccg
     2281 gcgagctgtt caaggcccgc accggcacct acatcgtgca catcccttac aaagggacgg
     2341 cggcctcact caccgccgtc atcgccgggg aagtgcactt cagctttgcc aacgtcccgg
     2401 ccatcctcgg ccacgtgcga agcggccggc tgaaggcgct cgcggtcctc gcgcccaagc
     2461 gctccgacct gatgcccgac gtgccgacga tggaggaagc gggggttgcc ggcgtcgagg
     2521 tcccggtctg gtacgccctg ctcggccccg ccgcgctgcc gcgcgacatc gtgaagaccc
     2581 tgaacgacgc caccgtgcgc gcggcgcgct cgcccgacct gagccaaaag ctcgtcgagc
     2641 agggcgccga gccggtcggc aacagtcccg aggagttctc gaagctgctg cgcgaggaag
     2701 tcgccaagtg ggccgcggtg gtgaaggttt ccggtgcgaa ggccgaatag gatgcaaaag
     2761 actcccgccg ccctggtggt ggcggagaac cgcgcaggcg cggcaggctc aatcggagca
     2821 gtcgcggtgg cgaagtcggt gcccgacggc tacaccctgc tgctcggctc gagcggcacc
     2881 gtcaccgccg gcccggcggt atggaagaac ctcgccttcg atccgctc
//
LOCUS       DASTPD010000066         4646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1458314, whole genome shotgun sequence.
ACCESSION   DASTPD010000066 DASTPD010000000
VERSION     DASTPD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458314"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR57_02525"
     CDS             complement(<1..206)
                     /locus_tag="VFR57_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU0258280.1"
                     /translation="MTSCADVLAGRAPGDAAVVLKGWVRTRRDSKAGFSFIHLSDGSS
                     FHPVQVVAPKDLPNYEAEVLRLTSG"
     gene            219..677
                     /locus_tag="VFR57_02530"
     CDS             219..677
                     /locus_tag="VFR57_02530"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEU0258281.1"
                     /translation="MKETRSILFICTGNICRSPTAEAVLKSLSLDAGIELRIESAGLG
                     DWHVGSPPDERTQHHARSRGYDLSAQRARQVRIQDFEEFDLILAMDRGHLQALRSMAP
                     AEHHSKIRLFVADADVPDPYYGGAAGFERVLDLVEEHCRSLLDELNRTTS"
     gene            complement(1015..3123)
                     /locus_tag="VFR57_02535"
                     /pseudo
     CDS             complement(1015..3123)
                     /locus_tag="VFR57_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
     gene            3380..4264
                     /locus_tag="VFR57_02540"
     CDS             3380..4264
                     /locus_tag="VFR57_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0258282.1"
                     /translation="MNDLSKARATWVEVGEESEAQRIDNFLLRALKGVPKSHVYRVVR
                     SGEVRVNSARVKPDYRLRAGDRIRVPPIRVSNRKPAARPHEFPIVYEDASILVIDKPS
                     GVAVHGGSGVSFGVIESLRAARPQAKVLELAHRLDRDTSGLLILAKKRSALVELHRML
                     REGEVEKDYLALVKGEYKGPPEIRAPLHKYVTGSGERRVAVTERGMFAATRVKVLKQA
                     AELSLLEARLLTGRTHQIRVHLAHVGHPILGDEKYGGGSAKRLLLHAAKLAFRHPLTG
                     EPLRLESPMPSAMQALVP"
     gene            4261..>4646
                     /locus_tag="VFR57_02545"
     CDS             4261..>4646
                     /locus_tag="VFR57_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0258283.1"
                     /translation="MKYRLLVFDWDGTIIDSASTIAECIRDASRDLSLEVPELERARH
                     VIGLGLHDAMRIVVPQLPASRYPEFVASYRKHFLARKDSMQLFAGMRELLEQLSGRHL
                     LAIATGKSRQGLDRDIEFHGLKALFAA"
BASE COUNT          718 a         1523 c         1537 g          868 t
ORIGIN      
        1 cccgaagtga ggcgcagtac ctcggcttcg tagttcggca ggtccttggg cgccacgacc
       61 tgcaccggat ggaacgagga gccgtccgag aggtgaatga acgaaaaacc ggccttcgag
      121 tcgcgccgtg tgcgcaccca gcccttgagc accaccgcgg cgtcgcccgg cgcgcggccg
      181 gcgaggacgt cggcgcagga agtcataatg ccgatgatat gaaagaaacg cgctccatcc
      241 tattcatctg caccggcaac atctgccgat cgcccaccgc ggaagcggtt ctgaaaagcc
      301 tcagtcttga cgcggggatc gagctgcgca tcgagtcggc gggcctgggc gactggcacg
      361 tcggcagccc gcccgacgag cgcacccagc accacgcgcg gagccgcggc tacgatctaa
      421 gcgcgcagcg cgcgcggcaa gtccgtatcc aggacttcga ggagttcgac ctcatcctcg
      481 cgatggaccg cggccatctg caagcgctaa gaagcatggc gcccgccgag catcactcga
      541 agatccgcct atttgtcgcc gacgccgacg tacccgatcc ctattacggc ggcgccgctg
      601 gcttcgagcg cgtgctagat ctcgtcgagg agcattgccg ctcgctcctc gatgaattga
      661 acagaactac ttcctagtct cgacctgctg cagctcgtcc tcctgcggcg caggcttcgg
      721 cttttcgcgc cgcggccggc cgagctgcgg cggcgcttcc tcagctgggg ctcccggcgc
      781 tttcgaccgg tcggtctcga tcatcaccag cccgtcgctt tccaaaagcg ctttggaatc
      841 gaccttcggc gtatcgatcc gcggcgccgc ctcctcgacg cgcggtgcgg cctgcgctgg
      901 cggcagagca tcctcggcgt gcagctcgtc cttgcgcgca agggcttcac ggcgctggtc
      961 ttcgcgcagc tggtcttcct ttatcgcgag tgcaggcgcg gtggcggctg gccgctcctc
     1021 gcgccgctcg cgatggcggt cccgccggtt gcgcttgccg ccacggcgct cgccgtcgct
     1081 ctcccgcggc tgctgtggct gttgcggctg ctgctgcggc cgctgttgct gctgctgagg
     1141 cgggcgctgc tgccgttgct gctgcggctg gccattgcgc cgctgctcga gccggccatg
     1201 ctgctgccgg tcgcgctgct cgtcgcgggg gcggccttcg cgtccgcggt cccgctctcc
     1261 gcgatgctcg ccgcggtcgc ggcgcggctg gtggtcccgg cgcggctgct gatcgcggcg
     1321 cggctcgcgc tgcggcgccg gttgtgccgc cggcggggtg gggatttgcg gctgcatggc
     1381 cggcgtggct ggcttgaaca ggccgaaaag cttcgatagg aaacttcctt cggcgactgg
     1441 ctgcggcacg ggctggggtt ccgcgaccgg ctggggctgg ggccgcggca acgactgatc
     1501 gacgaccggc gcgggctgcg agggggtaat cgccttgacc accgcctcct ggcgctcgcg
     1561 cttggtttct tcctcggcgc ccggcgcctt cggctcctcg ggccgctcga ccagctcgta
     1621 gctcgccggc acatgttcca tctggttcag ctcgtcgtgc ttgaggcgct gcaccttgta
     1681 gtgcggcgtc tcgaggtgcg ggttcggcac gagcacgatg ttcaccttca gccgagcctc
     1741 cagcttctgg atctcggcgc gcttctcgtt cagcaggaat gacgacacgt cgaccggcac
     1801 ctgcgcgtgc accgcgccgg tattctcctt catcgcctct tcctggacca tgcgcaaaac
     1861 gtgtagcgcg gtcgactcgg tgccgcggat gaagccggtg ccgctgcagc gcggacagct
     1921 gatatgcgcg gtttcttcca gcgacgcttg cagccgctgg cgcgaaagct ccatcaagcc
     1981 gaagcgcgag atcttgccga tctgaacgcg cgcgcggtcg tacttgagcg cgtcgcgcag
     2041 ccggttttcc acctcacgct ggtttcgctg cgactccatg tcgatgaagt cgatgacgat
     2101 cagccccccc aggtcgcgca gcctcagctg gcgcgcgact tcctcggcgg cttccaggtt
     2161 ggtgcggaac gcagtctcct cgatgtcgcc gccgcgcgtc gcgcgcgccg agttgacgtc
     2221 gatggcgacc agcgcctcgg tatggtcgat cacgatcgcg ccgcccgagg gtaaggcgac
     2281 ctggcgcgaa tatgcactct cgatctggtg ttcgatctga aagcgcgaga agagcgggat
     2341 gtcgtcgcgg taaagcttca ccttgccgac gttgcccggc atcaccgttt gcatgaatgc
     2401 cttcgcctgc tcgtagatct cctcggtgtc gatcaggatc tcgccgatgt cgggctggaa
     2461 gtagtcgcgg atcgcgcgga tcaccaggct cgactcgaga tagatgagga aaggcttctt
     2521 cgactggtcc tgcgacgcgc cttcgaccgc gcgccagagc tggagcaggt agttcagatc
     2581 ccagttcagc tcttcgagcg aacggccgat cgccgcggtg cgggcgatga cgctcatgcc
     2641 ttcggggagc tggagctggt ctagcgtgtc gcgaagctcg tttctgtcct ccccctcgac
     2701 tcggcgcgac accccgccgc cgcgagggtt attcggcatg aggacgaggt agcggccggc
     2761 gaggctgata aaggtggtga gcgccgcgcc cttggtgccg cgctcgtcct tctcgacctg
     2821 gacgatcagc tcctggcctt cgcgcaacgc atcctgaatg cgggcgcggc cggcgtcgat
     2881 gtcgggcttg aagtagctgc gggaaacttc cttgaacggc aggaatccgt gccgctcttc
     2941 gccgtagtcg acgaaagcgg cctcgaggct cggctcgatg cgggtgatga cccctttgta
     3001 gatgttgctt ttgcgttgct cccttgcagc ggattcgatg tccaggtcga cgagcttctg
     3061 cccatctacg atcgcgacgc gcagctcttc ctgctgcgtc gcgttaaaca acatgcgctt
     3121 cactttttgt tctcctgcgc gcgcaggaga gagtgtcagc cggctgaaaa aatctcctct
     3181 tcgggatgac ctctgggtcg cctgagactt ctgtgggaaa gcccccgcgc gcgctactag
     3241 tagtatcgct acttccggtg agcgaaatta accagcgctt caggctcggc tgcggccggg
     3301 caggaggcgg gttgcaggtt ccggcactgc cgggatcctg tgcctcgcaa ctcgaagcgg
     3361 cgaaaagatc atacggaaaa tgaatgactt aagcaaggcg cgggcgacct gggtggaagt
     3421 tggcgaagaa agcgaggcgc agcgcatcga caactttctg ttgcgcgcgc tgaagggagt
     3481 cccgaaaagc cacgtctacc gcgtggtccg cagcggggaa gtgcgcgtca acagcgcacg
     3541 ggtgaagccg gactacaggc tgcgcgccgg agaccggatc cgcgtgccac ccataagggt
     3601 ttccaatcgg aagcccgcgg cgcggccgca tgaattcccg atcgtgtatg aggatgcctc
     3661 gatcctcgtg atcgacaagc cctccggcgt cgccgtacac ggcggtagcg gcgtcagctt
     3721 cggggtcatc gaatccttgc gcgcggcccg gccgcaggca aaggtgctgg agcttgcgca
     3781 caggctggat cgcgatacct cggggcttct catcctcgcg aagaagcgaa gcgcactcgt
     3841 ggagctgcac cgcatgctgc gcgagggcga ggtggaaaag gactacctcg ccttggtcaa
     3901 gggggagtac aaggggccgc cggagatccg cgcgccgctg cacaagtacg ttaccggctc
     3961 gggcgagcgg cgcgtcgcgg tcaccgagcg cggcatgttc gcggcgacac gcgtaaaagt
     4021 ccttaagcaa gcggccgagc tcagtctgct ggaagcgcgt ctccttacgg gccggaccca
     4081 ccagatccgg gtgcacctcg cccacgtagg gcacccgatc cttggagacg aaaagtacgg
     4141 cggcggcagc gccaaacggc ttctcttgca cgctgcgaag cttgcttttc gccatcccct
     4201 gaccggcgag ccgttgcggc tcgagtcgcc gatgccttct gcaatgcagg cgctcgtgcc
     4261 gtgaaatacc ggcttctcgt gtttgactgg gacgggacca tcatcgactc cgcctccacg
     4321 atcgccgaat gcattcgcga cgcaagccgc gatctttctc tcgaagtacc cgagcttgag
     4381 cgtgcgcgcc acgtgatcgg gctgggcctg cacgacgcga tgcgcatcgt cgtaccgcag
     4441 ctgccggcca gccgataccc ggagttcgtc gcgagctatc ggaagcactt cctcgcgcgc
     4501 aaggactcca tgcagctctt cgcgggcatg cgggagctgc tagagcaact ttccggcagg
     4561 catcttctgg ccatcgcgac cgggaagagc cggcaggggc tcgaccgcga catcgagttc
     4621 cacggtctga aggctctgtt cgcggc
//
LOCUS       DASTPD010000067         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_235616, whole genome shotgun sequence.
ACCESSION   DASTPD010000067 DASTPD010000000
VERSION     DASTPD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235616"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..765)
                     /locus_tag="VFR57_02550"
     CDS             complement(121..765)
                     /locus_tag="VFR57_02550"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0258284.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEDAGASYINKDPANDPKVFAPP
                     YLGAGKLVIPQTANILLYLGPRLGLAPKNEATQVRLHGLQLTIADWLAEVHDTHHPIA
                     ASLYYEEQKPEALRRARIFLAERLPKFMRYFERAVDERFSYVHLSLFQMIEGLRYAFP
                     NAMRRLEKRHKKLIALHDRVAARPRIAAYLSSKRRIPFNQYGIFRRYPELDEGR"
     gene            825..1430
                     /locus_tag="VFR57_02555"
     CDS             825..1430
                     /locus_tag="VFR57_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEU0258285.1"
                     /translation="MARASVVSCDTCGLVQAMDRLPPGAAAECSRCGSFIAAGSSRRR
                     LEVVAALSLAALVLYVPANTYPILKMYLHGSYSESTVWDGVVTLMTHNEWAVAVIVFL
                     ASMVIPLVKLAGLFALVVTARMGWSRHLRERTWLYKFIDTIGPWAMLDVFLLAVLVAL
                     VKLSSWAKVIPGPGLVAFTAMVVLTMLASAAFDPKLIWERR"
     gene            1427..>2161
                     /locus_tag="VFR57_02560"
     CDS             1427..>2161
                     /locus_tag="VFR57_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0258286.1"
                     /translation="MSGEELPRARIRRRRLFRLVWVVPAVALAVAVYLVWQHMRAVGP
                     EIALRFSDASGVRAGQTPIIYRGVQVGEVIHVALAPDRSHALVRARLHKASAPLASEG
                     ALFWIVRPQLSLNQVTGLSTVLSGPEIHVLPGKGAQLQAEFTALEAAPAGIGMPGLRL
                     VLRAERPGGVRVNTPVNYRGVEVGLVQRLDLAPNSASADIHILIYSRYAPLVRAGSVF
                     WNTSAVQATGSILKGIELELESLRTLF"
BASE COUNT          330 a          670 c          751 g          410 t
ORIGIN      
        1 cggtcgttgt caggagctcc ggccggatct cgagctccgc cacgatgcgg tccttctccg
       61 tctgcacaaa gcgaatgccg aggtgcttca ccaggccgcc gttccagtcg atctgcattc
      121 tcagcgtcct tcgtcgagct caggataacg gcgaaagatc ccgtactggt tgaacgggat
      181 gcgccgtttc gaggacaggt acgcggcgat gcgcggcctc gccgcgaccc ggtcgtggag
      241 ggctatcaac ttcttgtgtc gcttctcgag tcgtctcatg gcgttcggaa aggcgtagcg
      301 cagtccctcg atcatctgaa ataacgaaag gtgcacatac gagaagcgct cgtctaccgc
      361 gcgctcgaag tagcgcatga actttggcag tcgctcggcc aggaagatgc gtgcgcgccg
      421 cagcgcctcg ggcttctgct cctcgtaata gaggctggcg gcgatcgggt ggtgagtgtc
      481 atgcacctcg gcgagccagt cggcgatcgt gagctgcagg ccatggagcc gcacctgggt
      541 cgcttcgttc ttgggcgcaa ggccgaggcg gggtcctagg tagagcagga tattcgcggt
      601 ctgggggatg acgagcttcc cggccccgag ataaggcggc gcgaacactt tcggatcgtt
      661 ggcagggtct ttgttgatgt aagaagcgcc ggcatcctcg agcgcgagcc gcacgaactc
      721 gccgcgcccc tggatggagg gccagtaata gagctcgtaa cgcatgctcc gaaaggtaca
      781 tgagggcacg ggtgcgcggg catgctgttt gcaaagcgat catgatggcc cgcgcatcgg
      841 tagtctcctg cgatacatgc ggcctggtgc aggcgatgga ccggctgccg cccggtgccg
      901 ccgccgaatg cagccggtgt ggctcgttca tcgcggccgg ctcgagccgc cgccggctcg
      961 aggtggtcgc cgcgctttca ctggcggcgc tggtgctcta cgtgcccgcc aacacttatc
     1021 ccatccttaa gatgtatctg cacggctcct actccgagag caccgtgtgg gatggcgtgg
     1081 taacgctcat gacgcacaac gagtgggccg tggccgtgat tgtgttcttg gcgagcatgg
     1141 tgattccgct ggtgaagctc gctggccttt tcgcactcgt agtgacggcg cgcatgggct
     1201 ggagccggca tttgcgcgag cgcacatggc tctacaagtt catcgacaca atcggcccct
     1261 gggcgatgct ggacgtgttc ctgctcgcgg tgctggtcgc gctggtgaag ctctccagct
     1321 gggcgaaggt gatcccgggt ccagggctgg tcgctttcac cgcgatggtt gtgctcacaa
     1381 tgctcgcttc ggccgcgttt gatccgaagc tcatctggga gcgccggtga gcggcgaaga
     1441 gctgccgcgg gcgaggatcc ggcgccggcg cctgttccgg ctggtgtggg tggtgccggc
     1501 ggtggcgctt gccgtcgccg tgtatctcgt gtggcagcac atgcgcgcgg tcgggcccga
     1561 gatcgcgctc cgcttcagtg acgcgagcgg cgtgcgtgca gggcagacgc cgatcatcta
     1621 ccgcggcgtg caggtgggcg aagtgatcca cgtcgccctc gcgcctgacc gaagccacgc
     1681 gctcgtgcgc gcacgcctgc acaaggcatc ggcgccgctc gcatccgaag gcgcgttgtt
     1741 ctggatcgtg cggccgcagc tgagcttgaa ccaggtgacc gggctttcga ccgtgctgag
     1801 cgggcccgaa atccatgtgt tgccgggcaa aggcgcgcag ctccaggccg agttcacggc
     1861 tctggaggca gcgccggcgg gcatcggcat gccgggattg cgactcgtgc tgcgcgccga
     1921 gcgccccggc ggggtgcggg tcaacacccc ggtgaactac cgcggcgtcg aggtcggcct
     1981 cgtgcagcgg ctcgacctcg cgcccaactc ggcttcggcg gacatccata tcctgatcta
     2041 cagccgctac gcgccgctgg tgcgcgcggg ctccgtgttc tggaacacga gcgccgtgca
     2101 ggcgaccggc agcatcctga agggcatcga gctcgagctc gagtcgctgc gcacgctttt
     2161 c
//
LOCUS       DASTPD010000068         9745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_235849, whole genome shotgun sequence.
ACCESSION   DASTPD010000068 DASTPD010000000
VERSION     DASTPD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235849"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1017)
                     /gene="ubiA"
                     /locus_tag="VFR57_02565"
     CDS             complement(136..1017)
                     /gene="ubiA"
                     /locus_tag="VFR57_02565"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEU0258287.1"
                     /translation="MSYASTVNLAIIARRLDAYERLIRLDKPIGALLLLWPTLWAVWL
                     ASGGTPQPDIVLIFLVGTVLMRSAGCALNDYADRRFDPHVERTRARPLAAGEIQPREA
                     LAVAAALCAAAFALVLFLSGLAILLSFLAVAIAAAYPYTKRFFALPQAVLGIAFGFGI
                     PMAYAAIQERLPLECWVLFASNLFYSFAYDTEYAMVDRDDDARIGIRTSALTLGSWDV
                     RAVMASYALMLALLVAVGPMHGLRWPYYAGLAAAAAMMIHHWRLLRDRSREGAFKAFM
                     HSNWVGAAIFVGILCGQ"
     gene            complement(1014..2996)
                     /gene="recG"
                     /locus_tag="VFR57_02570"
     CDS             complement(1014..2996)
                     /gene="recG"
                     /locus_tag="VFR57_02570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0258288.1"
                     /translation="MRASLEKGLHRLGLRSRADLALHLPLRHEDETFLTPVESAPEGT
                     PVCVEANVLKAQVMYRPRRQLVVHAEGLVLRFFNFYSSQLRQFERAAQARLRVRAFGE
                     VRRGFFGAEMAHPRYRIVDEGEPLPQTLTPIYPTTAGLSQAELRARVLEALEAEPLED
                     TLPAALRRRLILPDFRASIELLHRPQPGSDLSGAWRRMKFDELLAQQLSMRFAYRRRR
                     SRRAPVLKVNGALLNTFLSALPFRLTGAQDRAMKEILSDLGVPHPMQRLLQGDVGSGK
                     TIVAGIACLAAVDSGTQAAVMAPTEILSEQHWRKFNEWLGPLGVRLGYLRGGMSKNEK
                     KRILQARVLIGTHALIQKDVEFYKLGLAVVDEQHRFGVEQRLALRKKAASLVPHQLMM
                     SATPIPRTLAMTYYADLDVSTIDELPPGRRPITTKLLSEKRRGEVLARIREACGEGQQ
                     AYWVCPVIEESKAGLRTALETFENLRNQLSDLRVGLLHGRLSAEQKSAVMQAFMRNAI
                     QVLACTTVIEVGVDVPNASLMVIESAERFGLSQLHQLRGRVGRPGGKEERESVCILLF
                     GELQGNAPERLKAMFETSDGFEIARRDLEQRGPGEFLGERQSGVPLLRYADLERDNDL
                     VEVAVQVAPELIHADPRAARAHVARWLGSRHELTEA"
     gene            complement(2992..3378)
                     /locus_tag="VFR57_02575"
     CDS             complement(2992..3378)
                     /locus_tag="VFR57_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU0258289.1"
                     /translation="MKSPIQTRDAPEAIGTYSQAVKVASGGSTVYLSGQIGLDPATMQ
                     MAEGIDAQAHRVFRNLAAVAAAAGLSLDHAVRMTVYLTDLAHFARVNEIMAQYVREPY
                     PARAAVGVAGLPRGALVEVDAILHAD"
     gene            3397..3711
                     /locus_tag="VFR57_02580"
     CDS             3397..3711
                     /locus_tag="VFR57_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0258290.1"
                     /translation="MISQLEKLLGTPRDGALLRYSIGLEHAKAGNRERAIEYLREAVA
                     RDPLYSAAWKVLGKTLTELHQIDAALDAYRRGIEAAQKKGDRQAEKEMTVFARRLEKS
                     KT"
     gene            complement(3715..4092)
                     /locus_tag="VFR57_02585"
     CDS             complement(3715..4092)
                     /locus_tag="VFR57_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258291.1"
                     /translation="MPEERLRRIAIADSSPAFLTAAASYISNLPGYVLAGTSDKASQA
                     VTLVNSSGADVLLLDLGSHPIRGLEILRRVKAQQRAPVVVAMTLFHTREAAAAARGAG
                     ADALVGKECFVAGLREALARLFP"
     gene            complement(4184..4828)
                     /locus_tag="VFR57_02590"
     CDS             complement(4184..4828)
                     /locus_tag="VFR57_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258292.1"
                     /translation="MIRVLLADDHALVRAGIHSLLQGMREVQVVAEASSGEEALELAD
                     RQRPDVVLMDIAMKGITGLEAAARMRDRYPDVRVVILSMHSGEEYVLQALRAGAVGYL
                     LKDAATGELELALRSVMRGESWLSPSVSRQVVEGYMHRTGGEAAPEVLTARQREVLKL
                     VAGGKSTKEIAFLLNLSVKTVETHRAQIMERLGIRDVAGLVRYALRTGLVPPEG"
     gene            complement(4825..5958)
                     /locus_tag="VFR57_02595"
     CDS             complement(4825..5958)
                     /locus_tag="VFR57_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU0258293.1"
                     /translation="MTPLRLIAALLQRLVDKIEPHEGRYRMLFERSPLPMWVVDTQTL
                     RFLAVNDAALRLYEYSREEFLTMTALDIRGAAEDVLFREFVSREGGSVRHGVFRHQKK
                     TGDPVDIDGVGHLVAWRGRPARLVQINDITERLRTQQTLERLNRELEVSHERLRALSR
                     RLFEVQEEERRRLARDLHDDVGQALTALKMQLESLARSGGELTARSRVDDCVDTVQHT
                     LERVRQLSLSLRPPQLDDLGLAAAVRSHLDRQARVAGLQAHFETQDAPRDLAPDTETA
                     CFRVAQEAINNVLRHARARNLWVRLFTVGGRLAISVRDDGRGFDMESVRERSAKGTSL
                     GLIGMEERMALAGGSFELRSAPGQGTVLVATFPLPSAKERAVG"
     gene            6347..6472
                     /locus_tag="VFR57_02600"
     CDS             6347..6472
                     /locus_tag="VFR57_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258294.1"
                     /translation="MSQCQNLIGADRDKCLQDERAATTGSGAGAGTSAPPAGSMR"
     gene            complement(6596..7483)
                     /locus_tag="VFR57_02605"
     CDS             complement(6596..7483)
                     /locus_tag="VFR57_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258295.1"
                     /translation="MSKTRFGFWPRPWHIAAAGLSAEPVADCADVVEFINKHERALDG
                     WMHPPLGRRFSSALGLSVPWMPATAYGLPSTHELNLDTDDEALADFSIALIGFLDGMR
                     LVREGWNHFYKTPIEPAKLVDFVCTEDEMSRAVDMATRRWRGLDRDCQKWMFGAIHWY
                     CFTAAYEHQFERFGGLYIVLDTLYRVHVRLGGSAADTRAEQAAVLADAHRIPLPDWAQ
                     VHTIDNKDVSRLSTLRNEFFHEGRYGGEPIGFDAPVETITLDLEAFVTRVILGMLSID
                     CAYVRTPATTQQRHGLDVS"
     gene            complement(7809..8069)
                     /locus_tag="VFR57_02610"
     CDS             complement(7809..8069)
                     /locus_tag="VFR57_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258296.1"
                     /translation="MKSAVAFKYTPTFDRDVHAVFESSPNEQQRLASRLPSVKSQPET
                     ELEVLRLVEEYDDSDGLAPARGVMVAVMLSAPFWAAVIWWLI"
     gene            complement(8426..8941)
                     /locus_tag="VFR57_02615"
     CDS             complement(8426..8941)
                     /locus_tag="VFR57_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0258297.1"
                     /translation="MSAPGYHLWLKPPEPAHALFAATIDTLAQRLSAARFEPHITLLA
                     RLEGGEADHIRRSDALAEILRPFEVQLSAPAYGNQHFQCIYMLVRQSGDVIRANALAR
                     RLFERGEESFMPHLSLVYGTYPNAMKKQIVDALPESLRASFIVGALHLVKSHSDDPRD
                     WHEIHAAPLRG"
     gene            complement(8938..>9745)
                     /locus_tag="VFR57_02620"
     CDS             complement(8938..>9745)
                     /locus_tag="VFR57_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258298.1"
                     /translation="LIPAAGRGNRIAPLPCSKELYPIGFRLDPTNGELRPKVSVHYLL
                     EKLFAARIARAYVILRDGKWDIPAYLGDGAVVGMHLAYLVTGATIGPPDTLDRAYPFV
                     GGASIALGFPDMLFGPDDAYQRLLERLHGAGADIVVGLYRAHDCRKMDMVDFDDAGRV
                     RSVTLKPRSSSLQYAWLCAVWRPAFTEFMHRFVAAERKRLTRAPSAYRELDAQGDLPM
                     GAVIKAAVESGLEVHGVPFPDDSYIDIGTPDDLERAVTRFHSAGRSGGPT"
BASE COUNT         1841 a         3154 c         3100 g         1650 t
ORIGIN      
        1 tcatcgcccg cagcggcaga gtctccagat gagtatcgcc gcggtgcacg aggcacgtcg
       61 gatagagcgt caggtgcgtg ccgcgcagca tgccgctcgc aaacccgaag cgcgtaagcg
      121 cagggccggc gtcgctcatt gcccgcagag aatgccgacg aaaatcgcgg ccccgaccca
      181 attgctgtgc atgaaggcct tgaacgctcc ttcgcggctg cggtcgcgaa gaagccgcca
      241 atggtggatc atcatcgccg ccgcagctgc caacccggcg taataaggcc agcgcagccc
      301 gtgcatcggg ccaacggcga cgagcagcgc aagcatgagc gcgtagctcg ccatgacggc
      361 gcgcacatcc cagcttccga gcgtgagcgc ggaggtgcga atgccgatcc gcgcgtcgtc
      421 atcgcggtcg accatcgcat actcggtatc gtaggcgaac gaatagaaca gattcgacgc
      481 gaacagcacc cagcattcga gcggcaggcg ctcctgaatc gccgcatagg ccatcggaat
      541 tccaaagccg aaagcgatgc cgaggacggc ctgtggcaat gcgaaaaacc gcttcgtgta
      601 cggataggcg gcggcaatcg caaccgcgag aaaagagagc aggatcgcca gcccgctgag
      661 gaacaacacg agcgcgaacg cggctgcgca cagggcggcg gccacggcaa gcgcctcgcg
      721 cggctggatc tcgcccgcgg cgagcgggcg ggcccgcgtg cgctcgacgt ggggatcgaa
      781 gcgccggtcg gcatagtcgt tgagcgcgca gccggccgag cgcatgagga ctgtgccgac
      841 aaggaagatc agcacaatgt caggttgcgg cgtgcctccg gaggccagcc ataccgccca
      901 caaggttggc cacagcagca gcagcgcgcc gatcggcttg tccaagcgga tcaagcgctc
      961 gtacgcgtcg aggcgcctcg caatgatcgc caggttcacg gtggaagcat aactcacgcc
     1021 tccgtgagct cgtgccttga gccgagccag cgagccacgt gcgcgcgcgc cgcgcgcggg
     1081 tcggcgtgga tgagttccgg ggcaacctgc accgccacct cgaccagatc gttgtcgcgc
     1141 tcgaggtcgg cgtagcgcag cagcggcacg ccgctctggc gctctccgag gaattcgccg
     1201 gggccgcgct gctcgaggtc ccgacgtgcg atctcgaagc cgtcgctggt ctcgaacatc
     1261 gccttcagcc gctcaggtgc gttgccttgc agctcgccga agagcagaat gcaaacgctt
     1321 tcgcgctctt ctttgcctcc cgggcgtccg acccggccgc gcagctggtg cagctgcgaa
     1381 agaccgaagc gctccgcgct ctcgatcacc atgagggaag cattgggcac atcgacgccc
     1441 acctcgatca ccgtggtgca cgccagcacc tgaatggcgt tacgcatgaa agcctgcatc
     1501 acggcgctct tctgctccgc cgaaagcctt ccgtgcagca gcccgacgcg caggtcggac
     1561 aattggttcc tcaggttctc aaacgtctcg agcgctgtgc gcaagccggc tttcgactcc
     1621 tcgatgacgg ggcacaccca gtacgcctgc tggccttcgc cgcacgcctc gcggatgcgc
     1681 gccaggactt cgcccctcct tttttcggaa agcagctttg tcgtgatcgg gcggcggccc
     1741 ggcggaagct cgtcgatggt cgacacgtcc agatcggcgt agtacgtcat ggcgagcgtg
     1801 cgcggtatcg gcgtcgcgct catcatgagc tggtgcggca ccaggctcgc cgctttcttg
     1861 cgcaacgcga gccgctgctc gacgccgaac cgatgctgct cgtccacgac ggcgaggccc
     1921 agtttgtaaa actcgacgtc cttctgaatt agcgcatgcg ttccgatcag cacgcgcgcc
     1981 tgcaggattc gctttttctc gttcttgctc atcccaccgc gcaggtagcc gaggcgcacg
     2041 cccagcggcc cgagccactc gttgaacttc cgccagtgct gttcagagag gatctcggtc
     2101 ggcgccatca ccgcggcctg ggtacccgaa tccactgcgg cgagacaggc gatccccgcg
     2161 acgatggttt tgccgctgcc aacgtcgcct tgcagcagcc gctgcatggg atggggcact
     2221 cccaggtcgc tgaggatttc cttcatcgcg cggtcttgtg ccccagttag ccggaaaggc
     2281 agggccgaca gaaaggtgtt cagcaatgca ccgttcacct tcagtacggg cgccctgcgc
     2341 gagcggcgcc gccggtaggc gaagcgcatc gagagctgct gtgcgaggag ctcgtcgaac
     2401 ttcatgcgcc gccacgcgcc ggaaaggtcg ctacccggtt gcgggcggtg gaggagctcg
     2461 atacttgcgc ggaaatcggg aaggatcagt cgtcgccgca gggctgcggg cagcgtatct
     2521 tccagcggtt cggcctccag cgcctccagc acgcgagcac gcagttcggc ttggctcaag
     2581 ccagcggttg ttggatagat gggtgtgagc gtctgcggca gcggctcgcc ctcgtcgacg
     2641 atgcggtagc gcgggtgcgc catttcggcg ccgaaaaaac cgcggcgcac ttcgccgaat
     2701 gcacgaaccc gaaggcgcgc ctgcgcggcg cgctcaaact gccgcaattg actggaatag
     2761 aaattgaaaa agcgcagcac caatccttcg gcgtggacca cgagctggcg cctgggccgg
     2821 tacatgactt gcgccttcag tacattcgct tccacgcaca cgggcgtgcc ctccggcgcc
     2881 gattcgaccg gggtaaggaa ggtttcgtcc tcgtggcgca gcggaaggtg cagcgcgagg
     2941 tcggcccgtg accgcagccc gagccggtgg agtccttttt cgagtgatgc cctaatcggc
     3001 gtgcaaaatt gcgtcgacct cgaccagcgc gccgcgtggc agcccggcca cgccaacggc
     3061 cgcacgcgcc ggatagggct cacgcacgta ctgcgccatg atttcgttca cgcgtgcgaa
     3121 atgcgcgagg tcggtaaggt agaccgtcat gcgcaccgcg tgatcgaggc taagccccgc
     3181 cgccgccgcg accgcggcaa ggttccggaa cacgcggtgc gcctgagcat cgataccctc
     3241 ggccatctgc attgtggccg ggtcgagccc gatctggccc gacaggtaca ccgtcgagcc
     3301 gccacttgcg accttcactg cctgtgaata ggtgccgatg gcctcgggcg cgtcgcgcgt
     3361 ctggatcggg cttttcatga ggggtatctt agcctaatga tttcgcagct agaaaagctg
     3421 ctcgggacgc cgcgcgacgg cgcgctgcta cgctactcga tcggactcga gcatgccaag
     3481 gccggcaacc gggagcgggc gatcgaatac ctgcgcgagg cggtcgcgcg cgatccgctt
     3541 tattccgcgg cgtggaaggt gctgggcaag actctcaccg agttacacca gatcgatgcc
     3601 gcgctcgacg cttatcggcg gggtattgag gcggcgcaga agaaaggcga ccgccaggcc
     3661 gaaaaagaaa tgaccgtctt tgcccgaaga ctggaaaaaa gcaagaccta gaaactaggg
     3721 gaacagccgg gcgagcgctt cgcgcagacc ggcaacgaag cactccttgc cgacaagcgc
     3781 gtcggcgccg gcgcctcttg ccgcggccgc ggcttctcgc gtgtggaaaa gcgtcatcgc
     3841 gaccacgacc ggcgctcgtt gctgggcttt cacgcgacgc aagatctcca ggccgcgtat
     3901 ggggtgcgag cccagatcga gcagcagcac gtcagctccc gacgaattca ccagcgtaac
     3961 cgcctgcgac gccttgtctg acgtgcccgc gagcacgtag ccgggcagat tggatatgta
     4021 gctcgcagcg gcggtaagga acgccgggct gctgtcggcg atggcgatac ggcggagacg
     4081 ttcttcgggc attgcaggcc acattagaac gcgaaccgca caccagccat agggaagtcc
     4141 cctagctgcg ttaccaattt cctgaggaaa ccgcaaaacc ttgtcagcct tccggcggca
     4201 ccaggcccgt gcgcagggca taccgcacaa gccctgccac gtcacggatg ccaagccgct
     4261 ccatgatctg agcgcggtga gtctccacgg ttttcaccga gaggttgagc agaaaggcga
     4321 tctccttggt gctcttgccc cccgccacca gcttgagcac ctcgcgctgg cgcgcggtga
     4381 gaacttcggg cgccgcctcg cccccggtgc ggtgcatgta gccctcgacg acctggcgcg
     4441 agaccgaggg gcttagccag ctctcgccgc gcatcacgct gcgcagcgcg agctcaagct
     4501 cgccagtggc ggcgtccttc aggaggtagc cgactgcgcc ggcgcgaagc gcttggagca
     4561 cgtactcctc gccggagtgc atcgacagga tcacgacccg gacgtccggg tagcgatcgc
     4621 gcatccgcgc ggcggcctcg agcccggtaa tgcctttcat ggcgatgtcc atcagaacca
     4681 cgtccgggcg ttggcggtcg gcgagctcga gcgcctcctc gccgctcgag gcttcggcca
     4741 cgacctgcac ctcgcgcatc ccctgcagca gcgagtgaat acccgcgcgc accagcgcgt
     4801 gatcatcggc aagaagcacc cggatcatcc gaccgcgcgc tccttcgccg aaggaagcgg
     4861 gaaagtcgcc accagaaccg tgccctgccc cggcgccgag cgcagctcga acgagccgcc
     4921 cgcgagcgcc atgcgctcct ccattccgat cagcccgaga cttgttcctt tcgccgagcg
     4981 ttcgcgcacc gactccatat cgaagccgcg gccgtcatcg cgcaccgaga ttgccagcct
     5041 gccgccgacg gtgaacaggc gaacccacag gttgcgcgcg cgcgcatgac gcagcacatt
     5101 gttgatcgct tcctgtgcca cgcggaagca agcggtttcg gtgtcggggg cgagatcccg
     5161 gggagcgtcc tgcgtctcga agtgagcctg caggccggct acgcgcgcct gccggtcgag
     5221 atgagagcgc actgcggcgg cgaggccgag gtcatcgagc tgcggaggcc gcaggctgag
     5281 cgaaagctgg cgcacacgct cgagggtgtg ctgaacggta tcgacgcagt catccacgcg
     5341 agaacgcgcg gtcagctcgc cgccgctgcg agcaagcgac tcgagctgca tcttcagcgc
     5401 cgtaagcgcc tgcccgacgt cgtcgtgcag atcgcgcgcg aggcgcctgc gctcttcctc
     5461 ctgcacctcg aacagccgcc gcgacaacgc gcgtagccgc tcgtgcgata cctcaagctc
     5521 gcgattcagg cgttcgagcg tctgctgagt gcgcaggcgc tcggtgatgt cgttgatctg
     5581 caccagccgc gccggtcggc cgcgccatgc gaccaggtgg ccgacgccgt cgatgtcgac
     5641 cggatcgccc gtcttctttt ggtgccggaa gacgccgtgg cgcaccgagc cgccttcccg
     5701 gctcacaaac tcgcgaaaca agacatcttc cgccgcgccg cggatgtcga gcgcagtcat
     5761 cgtcaggaat tcctcgcgcg agtactcgta cagacgtaga gcggcgtcat tgacagcaag
     5821 aaaacgcagc gtctgcgtat ccacgaccca catgggcaag gggctgcgtt cgaacagcat
     5881 ccggtagcgt ccctcgtgcg gttcaatctt gtcgaccagg cgctgcagca gggcggcgat
     5941 gaggcgaagc ggggtcatcg agcgggaatg gtaacgctgg cacgcggttt gcgccggcac
     6001 ggtgcgcgcc gccgggcctt ttgtaacggc tccaggagaa cggcctatcg caagcgcggt
     6061 aggacaacgc ggcggcacgc agcttgcggc tggcggcgca ccgattcctt tgacggagaa
     6121 tgcaatgacc ctcaagtcag ccgcctctgc gatgttgctc gcgctcgcga gcgcagccta
     6181 tgcccagccc caatccggtt tgacgccgcc gagcatgacg ccctctgaca cgccagccgt
     6241 cacgccatcg caatcgccat cggtgacgcc gtcgcaggcg ccttctgcca ctccatctcc
     6301 atcttccggg gcgaccgcgc cgacggcacc gcgagctgtc ggcggcatga gccaatgcca
     6361 gaatctgatc ggcgccgaca gagacaagtg cttgcaggac gagcgggccg cgaccacggg
     6421 cagcggagcc ggcgcgggca ccagcgcgcc gcccgcgggc tcgatgcggt aatgatcgga
     6481 gcattgctcg ttgcggctat gctggctgcc gctgcctgtt aatgcgttct atcggcaacc
     6541 atagcagacg gtagcctgcg gcctgcacac cggtaacaag ccagcctaca agaacctatg
     6601 aaacatcgag gccatgcctc tgttgcgtag tggccggggt ccgcacgtag gcacaatcaa
     6661 tagacagcat ccctagaata acccgcgtta cgaaggcctc aaggtcaagg gtgatggttt
     6721 ctacgggggc atcgaagcct atgggctcac ccccgtaccg gccttcatga aagaactcgt
     6781 tccgtagagt agaaagacgc gacacgtcct tgttatcgat tgtgtggact tgtgcccaat
     6841 cgggaagagg gattctgtgg gcgtccgcga ggaccgctgc ttgctctgca cgagtgtccg
     6901 ccgcactacc gcctagtcgc acatgaacgc gatagagcgt atccaacacg atgtacaggc
     6961 caccgaatcg ttcgaattgg tgctcatagg ctgcggtgaa gcaataccaa tggatcgcgc
     7021 caaacatcca tttttgacaa tccctatcca gaccacgcca acggcgcgtg gccatgtcca
     7081 ctgcccgcga catctcgtcc tcagtgcaga cgaagtcaac cagcttcgcg ggctcgattg
     7141 gcgtcttgta gaagtgattc cacccttctc ggacgaggcg catgccgtcc aaaaagccaa
     7201 tcaaggcgat ggagaagtca gcgagagcct cgtcgtcagt gtccaggttc agctcgtgag
     7261 tggacggcag gccgtatgcc gttgcgggca tccatggaac agatagaccg agagccgagg
     7321 aaaagcgtcg cccaagcggc ggatgcatcc atccgtcaag cgctctttcg tgcttgttta
     7381 taaactcaac gacatcagcg cagtctgcaa ccggttcggc gctgagccct gctgctgcaa
     7441 tgtgccaagg gcgcggccaa aagccgaaac gggttttgct catgcccgcc gacacggtct
     7501 caggctggca gtgctatcgt ccactgactc gccctccctg tgtgcgctta taccggaaaa
     7561 ttctgtgagc gactcttgag gggtgaatgc atcaatggag tggaggggat cgaagagcgc
     7621 gcttaagtgg ccgggaaata aggattaatt atctcggtca cggcttgagg ccgaggccga
     7681 acctctcaca gaggagcggc taaccatgga aaccacatct gcacgggcgg cgtgctgccg
     7741 ctcgctaacg ggctggcaag cgaagagcca gcgttttggt ttgaggacgc agccggctgt
     7801 ggatggcgct agataagcca ccagatgacc gccgcccaga acggtgccga cagcatcacg
     7861 gctaccatca cgccccgggc cggggccagg ccgtcggagt cgtcgtactc ctcgacgagc
     7921 cttagtactt caagttcagt ttcgggctgg ctcttcaccg acggcaagcg gctggcgagc
     7981 cgttgttgct cgttgggcga gctctcgaag accgcgtgca cgtccctgtc gaaggtaggt
     8041 gtgtacttaa acgcgacggc cgatttcatg acgaactcct ctttggtgct ctagcgcgtc
     8101 gatccacacg agcgcggtag gcacttaggc actcttccgc gacgtgaagt actgcccgcg
     8161 cgattgccag gtgatcgtcc aggggatcaa gtttcagttt ccgggacggg agtgatccgg
     8221 tccggtatga actatggtcc ccagggcacg atcgctctag ctgcgtacat accatagcta
     8281 cgttggggcg tcgttcgcat gagacagccg aatcgaaccg aacggtatat tgcgcctact
     8341 actttacaaa gcttcatccg cacggcgggt gtagctgctt tggctcctcg cccggcgccg
     8401 gacgcgagca cgcaacctag gtgcttcaac cacgcagcgg cgccgcatgt atctcatgcc
     8461 aatcgcgcgg atcgtcgctg tgcgatttga ccaaatggag tgcgccgacg atgaacgagg
     8521 cgcgcaggct ttcgggaagc gcatccacga tctgcttctt cattgcgttc gggtatgtcc
     8581 cgtaaaccaa gctcagatgc ggcatgaagg actcttcgcc gcgctcgaaa agccgtcggg
     8641 ccagcgcgtt cgcgcgaatg acgtctccgc tctggcgcac gagcatgtag atgcactgga
     8701 aatgctgatt gccataagcg ggcgctgaca gctgcacttc gaaaggccgc aagatctcgg
     8761 cgagcgcatc gctccgtcga atgtggtccg cctctccacc ctcgagccgc gccaacagtg
     8821 tgatgtgcgg ctcgaagcgc gctgcgctga gacgctgcgc gagcgtatcg atcgtggctg
     8881 cgaaaagcgc gtgcgcgggc tcggggggtt ttagccaaag gtgatagccc ggcgcgctca
     8941 tgtcggtccg ccgctgcggc ccgccgaatg aaaacgcgtc acagcacgct cgaggtcgtc
     9001 cggcgtgccg atgtcgatgt acgaatcgtc gggaaaaggc acgccgtgca cctctaatcc
     9061 cgattccacc gcggccttga tgaccgcgcc catcggcaga tcgccctgtg catccagctc
     9121 ccgatatgcg cttggagcgc gcgtcaatcg cttgcgctcg gcggcgacga agcgatgcat
     9181 gaactcggtg aacgccggcc gccatacggc gcaaagccat gcgtattgaa ggcttgagga
     9241 acgcggtttc agcgtgacgg accgcactcg ccccgcgtcg tcgaaatcga ccatgtccat
     9301 cttgcggcaa tcatgcgcgc gataaagccc aaccacgatg tcggcaccgg cgccgtgcag
     9361 cctctcgagc aagcgttggt aggcatcgtc aggcccgaag agcatgtccg gaaacccaag
     9421 ggcaatcgat gcgccgccaa caaacgggta ggcacggtcc agcgtatctg gcggcccgat
     9481 agtcgctccg gtgaccagat aggcgaggtg catccctacg acggcgccat ccccaaggta
     9541 ggccggtatg tcccacttcc cgtcgcgcag gatcacgtac gcgcgcgcga tgcgagcggc
     9601 gaaaagcttt tccaagaggt aatgcacgga aaccttcggc cgcaattcgc cgttggtcgg
     9661 gtcgagccgg aagccgatgg ggtagagctc tttgctgcac ggcaacggcg cgatgcgatt
     9721 gccgcgtccc gcggcaggaa ttaac
//
LOCUS       DASTPD010000069         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1258896, whole genome shotgun sequence.
ACCESSION   DASTPD010000069 DASTPD010000000
VERSION     DASTPD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258896"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..993)
                     /locus_tag="VFR57_02625"
     CDS             complement(1..993)
                     /locus_tag="VFR57_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU0258299.1"
                     /translation="MQGTKSSRFTAVGSDSSDVQPPCWRRRTRSAPFLSSAALAALAA
                     GPLQAQQLEPRAYSNVPVGLNFFIAGYTYSSGGLATDPALPLDNAELDIHAPFVAYAR
                     AFDAWGKSAKFDAVLATACLSGSAQFEGVPVSRDICGLLDPALRVTVNLYGAPAMGLA
                     EFRSYRQDLIVGVSFQVGVPLGQYDPSRLVNLGTNRWMFHPEIGASKRFGSLTAEAAV
                     GASFYTTNDDFFGGRRREQDPIYSAQVHLIFEFRGGSWLALNGNYYAGGRATVDGVKK
                     NGELGNSRLGATFALPLDRNHSIKLHASEGISVRAGDDFTTFGVAWQYRWAAGF"
     gene            complement(887..1756)
                     /locus_tag="VFR57_02630"
     CDS             complement(887..1756)
                     /locus_tag="VFR57_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0258300.1"
                     /translation="MFKQANSSFRLLLAVLLLASGAVHAQALEGRLKRIAEKKTIAIA
                     YRADAIPFSFERDGQPAGYSIDLCKRVVNSIERAIKVQPLKIEWVPVTVQTRFEVVAK
                     GKADMECGSSTMTLTRMKDVDFSNAIFVESTGLLIKTASGLRSLSDMSGKTIAVVAGT
                     TNERAINAQLKRRQLDATVQPFKSRDEAMAALEAGKADAFASDRLLLVGAATKAKDPN
                     TLTLLADQLSLEPYGIVVPRGDTGMRIAVNTGLARIYAGDEIVEIYRRWFGQFGRPTA
                     LLEAAYALGAIPE"
     gene            1858..>2397
                     /locus_tag="VFR57_02635"
     CDS             1858..>2397
                     /locus_tag="VFR57_02635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU0258301.1"
                     /translation="MQILGICGSLRKASLNMAALRACQDLLPQGMTLRITSIADLPIF
                     NQDVFDAGIPEPAKRLRTEIAEADGVLIASPEYNFSLSAALKNAIDWASRPPNQSWQD
                     KPVAIFSVTPGPLGGARVQYDLRRILGQLWAHVLPRPEVFIGAGPSKFTDGKLTDETT
                     RRFLADLLAGFKPWVERMRV"
BASE COUNT          407 a          787 c          796 g          407 t
ORIGIN      
        1 ctagaagccg gccgcccagc ggtattgcca agcgaccccg aaggtcgtga agtcgtcgcc
       61 ggcgcgcacc gagatgcctt cgctcgcgtg cagcttgatg gaatgattcc ggtcgagcgg
      121 gagggcgaac gttgcaccga ggcgggaatt gcccagctcg ccgttctttt tcacgccgtc
      181 tacggtggcg cgtccgccgg catagtagtt gccgttaagc gcgagccagc tgccgcccct
      241 gaattcgaag atgaggtgca cctgggccga atagatcggg tcctgctcgc gccgtcggcc
      301 accgaaaaag tcgtcgttgg tggtatagaa gctcgctccc accgcggctt ctgccgtcag
      361 ggagccgaac cttttcgaag ccccgatttc cgggtggaac atccagcggt tcgtgccgag
      421 gttgacgagt cggctggggt cgtattgccc gagcggcacg ccgacctgga aactcacgcc
      481 cacgatcagg tcctgccggt agctgcggaa ctcggcaagt cccatcgccg gtgcgccgta
      541 aaggttgacc gttacgcgca acgccggatc gagcaagccg cagatgtccc gcgaaaccgg
      601 cacgccttcg aactgagcgg aacccgacaa gcaggcggtg gcgagcacgg cgtcgaattt
      661 cgccgacttg ccccaggcgt cgaacgcgcg cgcatacgcg acgaacggcg catggatgtc
      721 cagctcggcg ttgtccagcg gaagcgccgg atcggtcgcg aggccgcccg acgagtaggt
      781 gtagccggcg atgaagaaat tcagcccgac tgggacgttg gaatacgcgc gcggctcgag
      841 ctgctgcgcc tggaggggcc ctgcggcaag cgcggcgagc gccgcgctac tcaggaatgg
      901 cgccgagcgc gtacgccgcc tccagcaggg cggttggacg tccgaactgt ccgaaccaac
      961 ggcggtaaat ctcgacgatt tcgtcccctg catagatgcg cgcaaggccg gtgttgacgg
     1021 cgatcctcat cccggtgtcg ccgcgcggca cgacgatgcc gtacggctcc agcgagagct
     1081 ggtcggcgag gagcgtaagc gtgttcgggt ctttcgcctt ggtcgccgcg ccgacgagga
     1141 gcagccggtc gctcgcgaac gcgtcggcct tgcccgcctc cagcgcggcc atcgcttcgt
     1201 cgcgcgactt gaagggctgc acggtggcgt cgagctggcg gcgcttgagc tgcgcgttga
     1261 tcgcgcgctc gttggtggtg ccggccacga ccgcgatcgt cttccccgac atgtcggaaa
     1321 gcgagcgcag ccccgaagcc gtcttgatga gaagccccgt gctctcgacg aagatggcgt
     1381 tcgagaagtc gacgtccttc atgcgggtca gggtcatggt gctcgagccg cactccatat
     1441 cggctttgcc cttggccacc acctcgaacc gcgtctgcac ggtgaccggc acccattcaa
     1501 tcttgagcgg ctggaccttg atcgcgcgct cgatcgagtt gaccacgcgc ttgcacaggt
     1561 cgatcgagta gccggcgggc tgcccgtccc gctcgaacga gaacggaatg gcgtccgctc
     1621 ggtaggcgat ggcgatggtc ttcttctcgg cgatgcgctt caggcgccct tccagcgcct
     1681 gggcgtgcac cgcgccggac gcgagcagca gcacggcgag caggaggcgg aaagaggaat
     1741 ttgcttgttt gaacatggcg gctccctcga gtcggcgtat cgcggcgcca tgctagggct
     1801 cgaaacgccc gtcgaacatc ggaccttggt cttatcggac gcgctagact gcgcgccatg
     1861 caaatcctcg gtatctgcgg cagcctcaga aaagcctcgc tcaacatggc ggcgctgcgc
     1921 gcctgccagg atcttctgcc ccaaggcatg acgctacgca tcaccagtat cgccgacctg
     1981 ccgatcttca accaggacgt gttcgacgcc gggatacccg agccggcgaa acgcctgcgc
     2041 accgagatcg ccgaggcgga tggcgtgctc atcgcgagcc cggagtacaa cttctccctg
     2101 tccgcggcgc taaagaacgc gatcgactgg gcgtcgcgcc cgccgaacca gtcatggcag
     2161 gacaaaccag tggcgatctt ctcggtgacg cccgggccgc tcggcggcgc gcgcgttcag
     2221 tacgatttgc gccgcatcct cggccagctc tgggcgcacg tgctgccgcg ccccgaggtg
     2281 ttcattggcg ccgggcccag caaattcacc gacggcaagc tcaccgatga gacgacgcgc
     2341 aggttcctcg ccgacctcct cgccggcttt aagccttggg tcgagcggat gcgggtg
//
LOCUS       DASTPD010000070         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_35374, whole genome shotgun sequence.
ACCESSION   DASTPD010000070 DASTPD010000000
VERSION     DASTPD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35374"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFR57_02640"
     CDS             complement(<1..730)
                     /locus_tag="VFR57_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0258302.1"
                     /translation="MSDVAAYSVTDGIAVITMNNPPVNGLGNALRAALMTHLKNAEAD
                     PTVKGTVIIGSAKAFSGGADIREFGKPREKPDLFEVNDQQDAMTKPLVAAIGGFALGG
                     GLELALGCHYRVALPRAQLGLPEVKLGILPGSGGTQRLPRIIPMAEAVRMMTSGATIH
                     AEKAKELGLVDEIVTGDLLQGSLEFTKRIVSSNKGIRRIRDMKSRLDGEAKGFFAKVR
                     EQVAKESRGYPAPLEIVACAEAAAT"
     gene            complement(914..1891)
                     /gene="bioB"
                     /locus_tag="VFR57_02645"
     CDS             complement(914..1891)
                     /gene="bioB"
                     /locus_tag="VFR57_02645"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEU0258303.1"
                     /translation="MNPPREVVYPSAMNVKDVLGLFGLPFTELVYRAAGVHRAHFDPS
                     QIQLSTLLSVKTGGCPEDCAYCPQSRRYDTGVPDEEMLPLEDVIACAKTAKANGATRF
                     CMGAAWRGPKDRDLEKVAVFIREVKALGLETCVTLGMLKPGQAERLREAGLDYYNHNL
                     DTSPQFYGEIISTRTYRDRLETLERVRAAGIRVCCGGIVGMGESRADRAALIAQLASL
                     GPQPESVPINSLIQVKGTPLHGVDPIDPLEFVRTIAATRICMPKAWVRLSAGRESMPE
                     ALQALCFLAGANSVFYGDKLLTTPNPETLKDRALFERLGLRPTATGAAD"
     gene            1890..>2666
                     /locus_tag="VFR57_02650"
     CDS             1890..>2666
                     /locus_tag="VFR57_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0258304.1"
                     /translation="MHGLMMDQPLLISDLIRHADRNHGDTEIVSKTVEGGIHRYTYRE
                     AHARARRLARALAALGVKTADRVGTLAWNGYRHYELYYGISGIGAIINTINPRLFAEQ
                     IVYISNHAENQALFFDATFMPLVEKLAPQLDTVRHYVLLTDRAHMPKGSKFLCYEELL
                     ERQSDDYQWPSFDERTAAALCYTSGTTGNPKGALYQHRSTVIHAYAAALPDALDLSAL
                     DCVMPVVPMFHANAWSLPYACTLVGTKMVFPGPHLDGKSVY"
BASE COUNT          455 a          860 c          867 g          484 t
ORIGIN      
        1 acgtggccgc ggcctcggcg cacgccacga tctcgagcgg cgccgggtag ccgcgcgact
       61 ccttcgccac ctgctcgcgc accttggcga agaatccctt cgcttcgccg tccagccgag
      121 atttcatgtc tcggattcga cggatgcctt tgttgctcga aacaattctt ttggtaaatt
      181 ccaaagaacc ctggagcagg tcacccgtga cgatttcgtc gacgaggccg agctccttcg
      241 ccttctccgc atggatggtg gcgccggagg tcatcatgcg cactgcttcc gccattggga
      301 tgatgcgcgg caggcgctgc gtgccgcccg agccgggcag gatgccgagc ttcacctccg
      361 gcaggcccaa ttgcgcgcgg gggagcgcga cgcggtagtg gcagcccagg gcgagctcga
      421 ggccgccgcc gagcgcgaag ccgccgatcg ccgcgacgag cggcttggtc atcgcgtcct
      481 gctggtcgtt cacctcgaac aggtcgggct tctcgcgcgg cttgccgaac tcgcggatgt
      541 cggcgccgcc ggagaacgct ttcgccgagc cgatgatcac tgtgcccttg accgtcgggt
      601 cggcctcggc gttcttgagg tgcgtcatca gagcggcgcg cagcgcgttg cccagcccgt
      661 tcacgggcgg gttgttcatc gtgatgacgg caatgccgtc ggtaacggag taggcggcaa
      721 cgtcgctcat ttgggaaaga ccgggaaagg ggggctagcc gattttactg gcaaagtcgg
      781 cgcgcgttca tcgtcggttc gtcggacgaa gcgcaggagc gggcctcccg ggtggttgtc
      841 tgatggattg acttatggtg gtctaaccgg aacccgaaag tcgggccggt acatcctcgc
      901 gacgcgttga gcttcagtcc gccgcgcctg tagcggtcgg gcgcaatccc aggcgctcga
      961 aaagcgcgcg gtccttgagc gtttccgggt tcggggtagt gagaagcttg tcgccgtaga
     1021 acaccgagtt cgcgcctgcg aggaagcata gcgcctgcag tgcctcgggc atgctttcgc
     1081 gcccggcgga gagtcgcacc caagccttgg gcatgcagat gcgcgtcgcc gcaatggtgc
     1141 ggacaaattc gagcggatca ataggatcga cgccgtgaag cggcgtgcct ttgacttgaa
     1201 tcaatgaatt gatgggaacg ctttcgggtt gagggccgag gctcgcgagc tgcgcgatga
     1261 gcgcggcgcg atccgcgcgc gattcgccca ttcccacgat gccgccgcag cacacgcgga
     1321 tgccggcggc gcgtacgcgc tcgagcgtct ccaggcggtc gcggtaggtg cgcgtgctga
     1381 tgatctcgcc gtagaactgc ggcgaggtgt ccaggttatg gttgtagtag tcgagccccg
     1441 cttcgcgcag ccgctcggcc tggccgggtt tcagcatgcc gagagtcacg caggtttcca
     1501 gccccagcgc ctttacctct ctaataaaga cagcaacttt ctcgagatcc ctgtcctttg
     1561 gaccacgcca tgccgcgccc atgcagaagc gcgttgcacc attcgccttt gcggtctttg
     1621 cgcaagcgat gacatcctcc aggggcagca tttcctcatc cgggactccg gtgtcgtagc
     1681 ggcgcgactg cggacagtag gcgcaatcct cggggcagcc gccggtcttt accgaaagca
     1741 gggttgagag ctggatttgc gacggatcga aatgggcgcg atgcacgccg gcagcccggt
     1801 agacgagctc cgtgaacggc agcccgaaca gaccgagcac gtccttgacg ttcatggcgc
     1861 taggataaac tacttcgcga ggaggattca tgcacgggct gatgatggac cagccgctgc
     1921 tcatcagcga cttgatccgc cacgccgacc gcaaccacgg cgacactgag atcgtctcca
     1981 agacggtgga gggcggcatc caccgctaca cctaccgcga ggcgcatgcg cgcgcgcggc
     2041 gcctggccag ggcgctcgcg gcgctcggcg tgaagaccgc cgaccgggtc gggaccctcg
     2101 cctggaacgg ctaccggcac tacgagctct actacgggat ctcgggcatt ggcgcgatca
     2161 tcaatacgat caacccgcgg ctctttgccg agcagatcgt ctacatctcg aaccacgccg
     2221 agaaccaggc gctctttttc gacgcgacgt tcatgccgct cgtcgagaag ctcgcgccgc
     2281 agctcgacac cgtgcggcac tacgttctgc tcaccgatcg cgcgcacatg ccgaaggggt
     2341 cgaagttcct ttgctacgaa gagctgctgg aacggcagag cgacgactac cagtggccct
     2401 cgttcgacga gcgcaccgcc gccgcgcttt gctatacctc ggggacgacg gggaacccga
     2461 aaggcgcgct ctaccagcac cgcagcaccg tcatccatgc ctacgccgcc gcgctgcccg
     2521 atgcgctcga cctgtctgcg ctcgactgtg tcatgccggt ggtgccgatg ttccacgcca
     2581 atgcctggag cctgccctac gcgtgcacgc tggtcggcac gaagatggtg tttcccgggc
     2641 cacacctcga cggcaagagc gtctac
//
LOCUS       DASTPD010000071         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1260535, whole genome shotgun sequence.
ACCESSION   DASTPD010000071 DASTPD010000000
VERSION     DASTPD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260535"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="VFR57_02655"
     CDS             complement(<1..1552)
                     /locus_tag="VFR57_02655"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0258305.1"
                     /translation="MLELKDLSIDFGSRRVVHEASFTLDAGEKLALVGESASGKTVTA
                     LSTLRLIENARLSGQIIFNGESVLSMSAHELHQLRGRDIAVIFQEPMTALNPIYTVGD
                     QIVESLVLHTHLRGGAAWAEAVEALRRVGIDEPERRAHSYPHQLSGGQRQRAMIAMAL
                     ACHPKLLIADEPTTALDVTIRLQILELLERLRAEAGMALLLITHDLNLVRRFADRVAV
                     MEKGVLVEQGPTAQVIGRPQHPYTQKLVNSRPTREVTPPGKGLLVEAKNVRVDYPTRL
                     PGIRGWFKSGRFTAVESVDFDLAPGETLGVIGESGSGKTTLALAVLGLMRAQGDIRIA
                     NRGWGAGRVQRISMRKDIQVVFQDPLSSLSPRRTVEQIIGEGLEIHEPGLDAAARRER
                     VLQALYDVGLTEGGRAEPLLARYPHEFSGGQRQRIAIARALIVRPKVVVLDEPTSALD
                     VTIQKQVLELLSSLQKKYGLSYILVTHDIDVVRAMAHRVMVMKDSRIVESGPLEQVLE
                     RSRSDYTRR"
     gene            complement(1555..2637)
                     /locus_tag="VFR57_02660"
     CDS             complement(1555..2637)
                     /locus_tag="VFR57_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258306.1"
                     /translation="MSTPAVAVGFPVSASVHASPGRRAWRRFRANRLGYTSLVVFAVL
                     FGLSLLAEVLSNDKPLVARHEGKWHFPIVQTLPETSFGGDFATPTDYLDPLIQQNLSR
                     GDNFAVFPLNRYHHSTINYFASEPSPARPSRENLLGTDDRGRDLLARLVYGFRLSVVF
                     ALIVTAIISVIGTLYGAIQGYFAGWTDIAMERFNEIWSAMPTLYMLIIFSSLFSASFG
                     LLVVLLSLFGWLGIAAYVRAEFLRNRTLDYVRAARALGQSNWKIIRRHILPNSLTPVV
                     TLIPFEMAAAIGALTSLDFLGLGVPPGTPSLGELLNQGKNNLDAWWISLSTFGVLVVT
                     LLLLILIGDALRDALDPRKVLKEDEA"
     gene            complement(2641..>3001)
                     /locus_tag="VFR57_02665"
     CDS             complement(2641..>3001)
                     /locus_tag="VFR57_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0258307.1"
                     /translation="ILTKNSVLEEIRKHYVLTARAKGVDERRVLWRHVFRNAMIPIMT
                     GFPAAFVGAFFSGSLLIETLFSLDGLGLLSYESVIRRDYPVVFGSLFLFTIIGLFTTL
                     LRDLCYLWVDPRVKFFD"
BASE COUNT          576 a          957 c          979 g          489 t
ORIGIN      
        1 gccggcgcgt gtagtcggac ctcgagcgct caagcacctg ctcaagcggc ccactttcca
       61 cgatccggct gtccttcatc accatcacgc ggtgtgccat ggcgcgcacc acgtcgatat
      121 cgtgcgtgac caagatgtaa ctcaacccgt acttcttttg aagggaggac aagagctcga
      181 ggacttgctt ctggatcgtc acgtcgagcg cgctcgtggg ctcgtcgagc accaccacct
      241 tcggccttac gatgagagcg cgcgcgatcg cgatgcgctg acgctggccg cccgaaaact
      301 cgtgcggata gcgcgcgagc agcggctcgg cgcggccgcc ctcggtgagc ccgacatcgt
      361 agagcgcctg cagcacacgc tcccgccttg ctgcggcatc gagcccaggc tcgtggatct
      421 cgaggccctc gccgatgatt tgctctaccg tgcggcgcgg cgaaagcgag gacagaggat
      481 cctggaacac cacttgaata tccttgcgca tactgatgcg ctgcacacgc ccggcgcccc
      541 accctctgtt ggcgatgcga atatcccctt gcgcccgcat cagccccagt accgcaagcg
      601 ccagggttgt cttgcccgag cctgattcgc cgattacccc cagggtctcg ccgggcgcga
      661 ggtcgaagtc cacggactcc acggcggtga agcggccgct cttgaaccag cctcggatgc
      721 cggggagccg cgtcgggtaa tccacgcgca cgttcttcgc ctccacaaga agaccctttc
      781 caggaggagt aacttcgcgg gtcggccggc tgttgacgag cttctgggta tagggatgct
      841 gcggccgccc gatcacctgc gcggtcggcc cctgctccac cagcacgccc ttttccatca
      901 ccgccacgcg atcggcgaag cggcgcacga gattcaggtc gtgggtgatc agaagaagcg
      961 ccatgcccgc ctcggcgcgc aggcgctcca gaagctcgag gatctgcagt cggatcgtga
     1021 cgtcgagcgc ggtggtcggc tcatcggcga tcaggagctt cggatggcac gcgagcgcca
     1081 tcgcgatcat cgcgcgctgg cgctggccgc cggaaagctg gtgcggatag ctgtgcgcgc
     1141 gccgttccgg ctcgtcgatc cccacccggc gcagcgcctc cactgcctcc gcccacgctg
     1201 ccccgccgcg cagatgggta tgcagcacca gcgactcgac aatctggtcg ccgaccgtat
     1261 agatcggatt cagcgcggtc atcggctcct ggaagatcac cgcgatgtcc cggccgcgta
     1321 gctgatgcag ctcgtgcgca gacatgctca ggacgctttc cccattgaag atgatctggc
     1381 cgctgaggcg ggcgttctca atcaggcgca gagtggaaag cgcggtgacg gtcttgccgg
     1441 aagccgattc acctacgagc gcgagcttct cgcctgcatc cagagtgaag gaagcctcgt
     1501 gcacgacccg gcgggagccg aagtcgatac ttagatcctt cagctccagc aggctcacgc
     1561 ttcgtcctcc ttcaatacct tcctgggatc gagagcgtcc cgcagcgcgt ctccgatcag
     1621 gatgagaagc agcaacgtca cgaccaggac gccgaaggtc gacagggaga tccaccacgc
     1681 gtcgaggttg ttcttgccct ggttgagaag ctcgccgagg ctcggcgttc ccggcggcac
     1741 gccgaggccg aggaagtcca ggctggtaag ggcaccgatg gccgcggcca tctcgaaggg
     1801 aatgagcgtg accaccggcg tcagactgtt cggcagtatg tggcgccgga tgatcttcca
     1861 gttgctctgg ccgagcgcgc gggcggcgcg cacgtagtcg agggtgcggt tgcgcaggaa
     1921 ttctgcacgc acgtaggccg cgatgccgag ccagccgaag agcgagagca gcaccaccag
     1981 caggccgaag ctcgccgaga acagcgacga gaaaatgatc agcatgtaga gcgtcggcat
     2041 ggcgctccag atctcgttga agcgttccat cgcgatgtcc gtccagccgg cgaaatagcc
     2101 ttggatcgcg ccgtacaacg tcccgatcac ggaaatgatc gccgtaacga tgagcgcgaa
     2161 caccaccgag aggcgaaagc cgtagacaag ccgcgcgagc aggtcgcggc cccggtcgtc
     2221 ggtgccgagc aggttctcgc ggctcggccg ggccgggctc ggctcggaag cgaagtagtt
     2281 gatggtgctg tggtggtagc ggttgagcgg aaagacggcg aagttgtcgc cacgcgacag
     2341 gttctgctgg atcagcggat cgaggtagtc ggtgggagtg gcgaagtcgc cgccaaacga
     2401 tgtctcgggg agcgtctgca cgatcggaaa gtgccacttg ccctcatggc gagcgacgag
     2461 cggcttgtcg ttcgaaagca cttccgcgag caagctcagc ccgaagagca cggcgaacac
     2521 gacaaggctc gtatatccga ggcggtttgc gcggaagcgc cgccacgctc gccgcccggg
     2581 gctggcatga acgctcgctg aaaccggaaa accgactgca acggcgggag tcgacatgcg
     2641 tcagtcaaag aacttcacgc gcggatcgac ccagaggtag cagaggtccc tgagtagcgt
     2701 ggtgaacagt ccgatgatgg tgaagaggaa caggctgccg aacaccacgg ggtaatcccg
     2761 gcgaatgaca gactcgtacg aaagcagccc cagcccgtcg agcgagaaca gcgtctcaat
     2821 gagaagcgag ccgctgaaga acgcgccgac gaacgccgct gggaagccgg tcatgatcgg
     2881 gatcatggcg ttgcggaata cgtgccgcca cagcacccgc cgctcgtcca cgcccttcgc
     2941 gcgcgcggtg agcacgtaat gcttgcggat ctcctcgagc accgagttct tggtgaggat
     3001 g
//
LOCUS       DASTPD010000072         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1057562, whole genome shotgun sequence.
ACCESSION   DASTPD010000072 DASTPD010000000
VERSION     DASTPD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057562"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFR57_02670"
     CDS             complement(<1..553)
                     /locus_tag="VFR57_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0258308.1"
                     /translation="MAQEYFEIVFRIIAALVAGGMIGLERSHRGRPAGFRTHALVCLA
                     SSLLMLVTVYESHWFPRESAGRVILDPTRMAQGIMTGIGFVGAGAIIKEGFTVRGLTT
                     AASIWITAAIGILTGIGFYFAALIGTVLVLGTLSTFRWIEARLPTETYAIFMVRFRRE
                     ASMTEPALRELIESTGFSLHNLSY"
     gene            601..1962
                     /gene="glmU"
                     /locus_tag="VFR57_02675"
     CDS             601..1962
                     /gene="glmU"
                     /locus_tag="VFR57_02675"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0258309.1"
                     /translation="MALEILILAAGLGKRMRSSLPKVLHPLAGRPLLAHVLDTARELS
                     PRRIVVVHGHGAAQVKETFPQEDLEWVLQAEQLGTAHAVMQAMPRVSPDAQVLLLYGD
                     VPLVRVATLRRLLEAAGDGLALLTAELEDPAAYGRVVRDVAGRVKRVVELRDASPVER
                     AIREINAGFYALSAKRLSAWLKRIDNRNAQKEYYLTDLVALAVGDGVPVAAVKVEDAW
                     EAAGVNSKAELAALERRYQEIVSRKLMEMGVTLADPARIDVRGTLECGRDVLIDVNCV
                     FHGRVTLGDGVRVGANSILRNVSVAAGSEILPFCHLEDSIIGERCRIGPYTRLRPGNA
                     LAEGVHLGNFVEVKASRLGAGSKANHLTYLGDSEIGARVNVGAGTITCNYDGAAKHRT
                     VIEDDCFIGSDATLVAPVRIARGSYIGAGSTINKDTPADQLTVARARQVSIPAWKRRR
                     TKT"
     gene            1967..>3709
                     /gene="glmS"
                     /locus_tag="VFR57_02680"
     CDS             1967..>3709
                     /gene="glmS"
                     /locus_tag="VFR57_02680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU0258310.1"
                     /translation="MCGIVGAVARRDIVPVLIEGLRRLEYRGYDSCGLALVNAGSLDL
                     IRTVSRVDDLAARTQKTTGKTGIAHTRWATHGAPATENAHPIVSRGEIAVVHNGIIEN
                     HDELRAELQAKSYQFASQTDTEVIAHLVHSLHHGDLRDAVRRAAQRLKGAYAIAVVSK
                     KDPGTVVGARAGSPLVVGVGQNEHFVASDALALAGTTDRIAYLEEGDVVAINERGWSV
                     VGADGRAVTRELRTVKVSGAAAELGPYRHFMQKEIFEQPRAVADTLQSVTAVTAKLFG
                     DDAERQLRNVRSVLILACGTSYYSGLVARFWIEELAGIPCNVEIASEYRYRTSVPDAN
                     ALVVVISQSGETADTLSALKHAQSLGQECTLAICNVASSALMRMTRLQFLTHAGVEIG
                     VASTKAFTTQLCALFLLSLVLGKLRGRLDPQEESKHLKALRHLPTALGAVLALEPSII
                     AWAQRFAVREDALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVTGE
                     MPVVTVAPNDALLEKLKSNMQEVRARGGELYVFADADTKISSSEGMHVVRLPEHYGLL
                     SPILHVVPLQLLAYH"
BASE COUNT          659 a         1194 c         1249 g          607 t
ORIGIN      
        1 ggtagctcag gttgtggagc gaaaagccgg tagactcgat cagctcacgc agcgccggct
       61 ccgtcatcga cgcctcgcgt ctaaagcgca ccatgaagat ggcgtaggtc tcggtcggca
      121 ggcgcgcttc gatccagcgg aaggtcgaga gcgtgccgag cacgagaacc gtgccgatga
      181 gcgcagcgaa gtaaaaaccg atgccggtca ggatcccgat cgccgcggtg atccagatcg
      241 acgctgcggt ggtcaggccg cgcacggtga agccctcctt gatgatcgcg cccgcgccga
      301 cgaagccgat gcccgtcatg atgccctggg ccatgcgggt cgggtcgaga atcacgcgcc
      361 cggcggattc tcgcggaaac caatggctct cgtagacggt aacgagcatg agcaggctcg
      421 aagcgagaca aacgagcgcg tgcgtgcgaa aaccggcggg acgcccgcgg tggctgcgct
      481 cgaggccgat cattccgccg gcgaccagcg ccgcgatgat gcggaacacg atctcgaaat
      541 actcctgggc catgggccga gtctaggtga aaacgctaga atcggcacgg aatcagacgg
      601 atggcgctag agatcctcat tctcgcggcg gggctgggca agcgcatgcg ctccagcctt
      661 cccaaggtac tgcacccgct tgccggccgg ccgttgcttg cgcacgttct ggacaccgcg
      721 cgcgagcttt caccgcgccg gatcgtcgtc gttcatgggc acggcgctgc gcaagtgaaa
      781 gaaacgtttc cgcaagagga cctcgagtgg gtgctgcagg ccgagcagct cggcacggcc
      841 catgcggtca tgcaggcgat gccgcgggtg agcccggacg cccaagtgct tctgctctat
      901 ggtgacgtgc cgctcgtgcg tgtggcgacg ctcaggcgcc tgctcgaggc cgccggcgac
      961 gggctcgctc tcctcaccgc cgagctcgag gacccggccg cttatggccg ggtagtgcgt
     1021 gatgtcgcgg ggcgcgtgaa gcgcgtggtc gagctgcgcg acgcctctcc ggtggagcgc
     1081 gcaatccgcg agatcaatgc cgggttctat gcgcttagcg cgaaacggct ctcggcgtgg
     1141 ctcaaaagga tcgacaaccg caacgcgcag aaggagtact acctcactga tctggttgct
     1201 ctggcagtcg gagacggcgt gccggtggcc gcggtgaagg tggaggacgc ctgggaggcg
     1261 gccggagtga actccaaggc cgagctcgcc gccctggaac ggcgctacca agagatcgtc
     1321 tcccggaaac tcatggaaat gggcgtcacg ctcgccgacc cggcgcgcat cgacgtgcgc
     1381 ggcacgcttg aatgcggccg cgacgtcttg atcgacgtca actgcgtgtt ccacggccgc
     1441 gtgacgctcg gcgatggcgt gcgcgtgggc gcaaattcca tcctgaggaa cgtctcggtg
     1501 gcagcgggca gcgaaatcct gcccttctgc cacctcgagg actcgattat cggcgagcgt
     1561 tgccgcatcg gcccgtatac gcgcttgcgc cccggcaacg cgctcgccga gggggtgcac
     1621 ctcggcaact tcgtcgaagt gaaggcgagc cgcctcggag ctggatcaaa ggcgaatcac
     1681 ctcacgtatc tgggcgacag cgagatcggg gcgcgggtga acgtcggcgc cgggacgatc
     1741 acctgtaact acgacggtgc ggccaagcac cgcacggtga tcgaggacga ctgcttcatc
     1801 ggctcggacg ccacgctggt cgcgccggtg cgcatcgcgc gcggctccta catcggcgcc
     1861 ggctcgacca tcaacaagga cacgcccgcg gaccagctca ccgtggcgcg cgcacggcag
     1921 gtttccattc cggcctggaa gcggcgccgg acaaagacct gatcgtatgt gcggcatcgt
     1981 tggtgccgtg gcgcggcgcg acatcgttcc ggtgctaatc gaggggctgc ggcggctcga
     2041 ataccgaggc tacgactcgt gcgggcttgc cctggtgaac gcgggcagcc tcgacctgat
     2101 tcggaccgtg tcgcgggtcg acgacctcgc ggcaaggacc cagaaaacga ctggcaagac
     2161 cggcattgcc catacccggt gggcgacgca cggggcgccc gcgaccgaga acgcccatcc
     2221 gatcgtgtct cgcggcgaaa tcgcggtagt gcacaacggg atcatcgaaa accatgacga
     2281 attgcgcgcc gaactgcagg cgaagagcta tcagttcgcg agccagaccg acaccgaagt
     2341 gatcgcgcac ctcgttcaca gcctccacca cggagacctt cgcgacgccg tgcgccgggc
     2401 cgcgcaacgc ctgaaaggcg cctatgccat cgccgtagtg tcgaagaagg atccgggcac
     2461 cgtggtgggc gcgcgcgccg gcagcccgct cgtcgtcggg gtcgggcaga acgagcactt
     2521 cgttgcttcc gatgcgctcg cgctcgccgg gacaacggat cgcatcgcct atctcgagga
     2581 aggcgacgtt gtggcgatca acgagcgcgg atggtcggtc gtgggtgcgg atggacgcgc
     2641 ggtaacccgg gagttgcgca cggtcaaggt gagcggcgct gccgccgagc tcgggcccta
     2701 ccggcatttc atgcagaagg agattttcga gcagccccgc gcggtcgccg atacgctcca
     2761 gagcgtgaca gcagtcaccg cgaaactgtt tggcgatgac gccgagcgcc agctgcgcaa
     2821 cgtccgttcg gtccttattc tggcatgcgg cacgagctac tactcgggcc tcgtggcccg
     2881 gttctggatc gaggagctcg cgggcattcc gtgcaacgtc gagatcgcga gcgaatatcg
     2941 ctaccgcacg agcgtcccgg acgccaacgc cctcgtcgtg gtgatctcgc agtccggcga
     3001 gaccgccgat actctctcgg cgctgaagca cgcccaatcg ctcggccagg aatgcacgct
     3061 cgccatctgc aacgtggctt ctagcgcgct catgcgcatg acgcggcttc agttcctgac
     3121 acatgccggc gtcgagattg gcgtggcctc cacgaaggcg ttcaccacgc agctgtgcgc
     3181 gctctttctg ctctcattgg tactgggcaa gttgcgcggc cggctcgatc cgcaggagga
     3241 atcgaagcat ctcaaggcgc tgcgccatct gccgaccgcg ctcggcgcgg tgctcgcgct
     3301 cgaaccctcc atcatcgctt gggcacagcg gtttgccgtc cgggaggacg cgctttttct
     3361 cggccgcggg ctgcactacc cgatcgcgct cgagggggca ttgaagctga aggagatctc
     3421 ctacattcac gccgaggcct atccggccgg ggagctcaag cacgggccgc tcgcgctcgt
     3481 caccggcgag atgccggtcg tgaccgtggc gccgaacgac gcgctcctgg agaagctcaa
     3541 gtccaacatg caggaagtgc gggcgcgtgg cggtgagcta tacgtattcg cggacgccga
     3601 tacgaaaatc tcttcttctg aaggaatgca tgtcgtccgg ctgccggagc attacgggct
     3661 gctgtcgcca atccttcatg tcgtcccgct acagttgctc gcctaccat
//
LOCUS       DASTPD010000073         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1058386, whole genome shotgun sequence.
ACCESSION   DASTPD010000073 DASTPD010000000
VERSION     DASTPD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058386"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFR57_02685"
     CDS             <1..219
                     /locus_tag="VFR57_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258311.1"
                     /translation="TVAQFRFDRGRAEGWTFGAAGTPEMQARLGATTYEVTVAMDAGE
                     SRTLRTREAHRFHIGQRVAVRGGELEPM"
     gene            247..504
                     /locus_tag="VFR57_02690"
     CDS             247..504
                     /locus_tag="VFR57_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258312.1"
                     /translation="MAEPRRLIIHFTDGTKKLLEFPQQIADADATLAARLKEALEARH
                     LVIEADGALVVIPFENIRYLQAYPAPKKLPSYAIKGATFRD"
     gene            507..2246
                     /locus_tag="VFR57_02695"
     CDS             507..2246
                     /locus_tag="VFR57_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0258313.1"
                     /translation="MDKRNFLRVAGLGALALGLGTREKALGQGQSAKPTFKGPYNFLF
                     ILTDQERFFRPGELPEGYRLPAHERLAKRGTRFVNHRINSCVCTPSRSVLYTGQHIQH
                     TRMFDNTNFPWIGSLSTDIRTVGDLLREAGYYTAYKGKWHLTKEFETVNKLAAPARIF
                     TKEMEDYGFSDYFGIGDIIAHTQGGYLHDGVIAAMGGSWLRGKGRELAAQGKPWFLAV
                     NLVNPHDVMFYDTDAPGAPFRPERGITHVARDPVDPLYAKKWSFKLPASYAQALDAPG
                     RPPAHRDFLRSHDALVGEIPNDEPRWRRRHNYYLNCLRDVDRNIGALLAELDASGLAN
                     RTIIILTADHGDMDGAHQLHAKGAVAYREQNHVPLIVAHPAYAGGRRCHAVTSHLDIA
                     PTLVGLTGAPAEKRAAIVKGLSGKDFSRLLADPEKAPLDAVRDGALFNYNMFAYLDGD
                     YLHKAVAHMQKGGNPKELKTAGIVPAVMKRGAVRSVYDGRHVFARYFSPKQHNRPASL
                     EELFRFNDVELYDVQSDPHEMKNLSANRELVAAMNEKLNRLIDAEVGEDRGQMLPGGI
                     DAGWEINRETMAP"
BASE COUNT          453 a          802 c          733 g          356 t
ORIGIN      
        1 accgtggcgc agttccgctt cgaccgcggc cgcgccgagg gctggacctt cggcgccgcc
       61 ggaacgccgg aaatgcaggc gcgcctcggc gcgaccacgt acgaggtcac ggtcgcgatg
      121 gacgcgggcg aaagccgaac gctgcgcacg cgcgaagcgc accgcttcca catcggccag
      181 cgcgtggccg tgcggggcgg cgagctcgaa ccgatgtaga aacgaaagac aaggggagaa
      241 gacaacatgg ccgaaccgcg ccgcctgatc attcacttca ccgacggcac gaagaagctg
      301 cttgaatttc cgcagcagat cgccgatgcc gacgccaccc tcgccgcgcg gctcaaggaa
      361 gcgctcgaag cgcgccacct cgtgatcgag gccgacggcg cgctcgtggt gatcccgttc
      421 gagaacatcc gctatctgca ggcctacccg gcgccgaaga agcttccgag ctacgccatc
      481 aaaggcgcca cgttcaggga ctgagcatgg acaagcgcaa tttccttcgc gttgccggcc
      541 tgggcgcgct ggcgcttggg ttaggaacaa gagaaaaagc tcttggacaa ggccaaagcg
      601 cgaaaccaac cttcaaaggc ccctacaact tcctcttcat cctgaccgac caggagcgct
      661 ttttccggcc gggcgagctg ccggaaggtt atcgcttgcc ggcgcacgag cggctcgcga
      721 agcgcggcac gaggttcgtg aaccaccgca tcaactcctg cgtgtgcacg ccctcgcgct
      781 cagtgctcta caccggccag cacattcagc acacgcggat gttcgacaac acgaacttcc
      841 cctggatcgg aagcctctcg accgacatcc gcaccgtcgg cgatctgctg cgcgaggcgg
      901 gctactacac cgcttacaaa gggaaatggc acctgacgaa ggagttcgag acggtcaaca
      961 agctcgccgc gccggcgcgg atcttcacga aagaaatgga ggactacggg ttctccgact
     1021 acttcggcat cggcgacatc atcgcccaca cccagggtgg atacctccac gacggcgtca
     1081 tcgccgccat gggcgggagt tggctgcgcg gcaaggggcg cgagctcgcg gcgcagggca
     1141 agccctggtt cctcgccgtc aacctggtga acccgcacga cgtgatgttt tacgacaccg
     1201 acgccccggg tgctccgttc cggcccgagc gcggcattac gcacgtcgcg cgcgaccccg
     1261 tggatccgct gtacgcgaag aagtggagct tcaagcttcc ggcgagctac gcgcaagcgc
     1321 tggatgcgcc cggccgcccg cccgcgcacc gcgatttcct gcgctcgcac gacgcgctgg
     1381 ttggcgagat accgaacgac gagcctcgct ggcggcgtcg ccacaactat tacctgaact
     1441 gcctgcgcga cgtcgaccgc aacatcggcg ccctgctcgc cgaactggac gcatccggcc
     1501 ttgccaatcg cacgatcatc attctcaccg cggaccacgg cgacatggac ggcgcgcacc
     1561 agctgcacgc gaaaggcgcg gtcgcctacc gcgagcagaa ccatgtgccg ctcatcgtcg
     1621 cgcacccggc ttacgcgggc ggcaggcgct gccacgcggt cacttcgcac ctcgacatcg
     1681 cgccgacact ggtcgggctg accggcgcgc cggcggagaa gcgcgccgcg atcgtcaagg
     1741 ggctctcggg caaggacttc tcacgcctgc tcgccgatcc cgagaaggcc ccgctcgatg
     1801 cggtgcgcga cggggcgctt ttcaactaca acatgttcgc ctacctcgac ggcgactacc
     1861 tccacaaggc ggtcgcgcac atgcagaagg gaggcaatcc gaaagagctc aagactgccg
     1921 gcatcgttcc cgccgttatg aagcgcggcg cggtgcggtc ggtgtatgac gggcgccacg
     1981 tgttcgcgcg ttatttttct cccaagcaac acaaccggcc ggcaagcctg gaagagctct
     2041 tccgcttcaa cgacgtcgag ctctacgacg tgcagtcgga cccgcacgag atgaaaaacc
     2101 tctcggcaaa tcgcgagctt gtggccgcga tgaacgaaaa gctcaatcgc ctgatcgacg
     2161 ccgaagtggg cgaggaccgc ggccagatgc ttccgggcgg gatcgacgcc ggctgggaga
     2221 tcaaccggga gacgatggcg ccctgacgca ggtggtttag cgttgacgct ctcacccctg
     2281 cccgggggct tgggtgcgcc acatgccatt cacgtgctcg tggcggttcg cttcatcaat
     2341 gccc
//
LOCUS       DASTPD010000074         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_854494, whole genome shotgun sequence.
ACCESSION   DASTPD010000074 DASTPD010000000
VERSION     DASTPD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854494"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFR57_02700"
     CDS             <1..247
                     /locus_tag="VFR57_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0258314.1"
                     /translation="AAEWGPKNVRVNAIAPGLVRTEFARALWEDEKRRADRIAATPLR
                     RLGEPRDIGGIAVFLASPAAAFITGQCIVADGGVTIL"
     gene            247..1359
                     /locus_tag="VFR57_02705"
     CDS             247..1359
                     /locus_tag="VFR57_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0258315.1"
                     /translation="MDLNYSAEELAFRDDVRAWLAANLPQELKDRGHLSKEELMRWHR
                     ILAKKGWVAPAWPKEWGGTGWNVVQRYIFEEELGYAGAPPIIPFGINMCAPVLLAFGT
                     EAQKKRFLPRIYNGDDFWCQGYSEPGSGSDLASLKTKAEKKENHYVVNGQKTWTSYAH
                     YADWIFCLVRTDPTVEKRQEGISFLLMDMRSPGITVRPLILMDGAHAVNEVFFDDVKV
                     PVENLVHKENQGWTVAKYLLGYERMNTGRIGESKRELRKLKENFSSKLSDVRFRDRLT
                     RVEVELMALEITNLRFLDRMRRTGQPPGADVSMLKIKGTEIQQALTELAIQAADPAAR
                     ETQALERRYLDMRKTTIYAGSNEIQRNIIAKMTLGL"
     gene            1356..2342
                     /locus_tag="VFR57_02710"
     CDS             1356..2342
                     /locus_tag="VFR57_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0258316.1"
                     /translation="MNFDYNEEQQLLADSVRRYLQKDYDFEARKKILATPEGWSERVW
                     ATFAEMGLTGLPLSPDYGGFGGGAVDLMSVMEAFGEALVVEPYLPTILAALAIAKGGD
                     AKQKQEFLPLVVQGKMRLALATGPRGVIGAPMADRIVFGSDGRLRIVARDAIEVRAYR
                     TLDDLAAGDIRFDGKGEPLAGGVLDEVLDFGAALSCAEAVGAMKFACDTTLEYLKTRR
                     QFGVPIGAFQALQHRMVDMYISYEQAKSMACLACSKADSALDARERARAVSAARIKIA
                     DAARHISQESVQLHGGMGMSDELKVSHTFRRLTALGRRFGDVDQHLARFASL"
     gene            2455..2904
                     /locus_tag="VFR57_02715"
     CDS             2455..2904
                     /locus_tag="VFR57_02715"
                     /inference="COORDINATES: protein motif:HMM:NF028494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258317.1"
                     /translation="MDEPNCPGCLAVTLTLRPDGAYLLRDQLGASEFYDFGRWRYADG
                     VLELAGDRDTRRYPLEKLKKSSQVEQLRGPFRMVGVYDGATFKECRTGLAWRLADTRA
                     AQALKGEVVKAQGNPVLVALDAQFEGSPEMLQVFRPAAVLDARGCPS"
     gene            2915..3889
                     /locus_tag="VFR57_02720"
     CDS             2915..3889
                     /locus_tag="VFR57_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0258318.1"
                     /translation="MENQRIVLASHPKGWVTPENFRIEKAQVPAPADGEVLVKNLWLS
                     LDPYMRGRISEAKSYVKGVDIGEVMVGQTVGEVIDSRNRNLKIGDHVLTQTGWQLYGV
                     TKEATKVDASRAPLSYYQGVLGMPGMTAYFGLKDIGQPKPAETVVVSAASGAVGSVVG
                     QLAKLWGCRAIGIAGGREKCDYVTRELGFDACIDYKSANLRDELKDVDVYFDNVGGEM
                     LDILLARMKLFGRIVVCGMIADYNATEPYRLKNLRAVLVNRLKLQGMIVFDWKERYGE
                     ALKALGGYYAAGKLKTRESIVEGLANAPQGLVALLKGGNFGKQLVKLA"
     gene            3861..4295
                     /locus_tag="VFR57_02725"
     CDS             3861..4295
                     /locus_tag="VFR57_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0258319.1"
                     /translation="MASSWSSSPDYAGFWQRAAALVIDWLIVTVIAVPIMVLGFGIPQ
                     LSLNPAEHSWDLLSFVAVAVAVIGFWRYCGATPGKIAVGLKIVDAATGAPPTLGRLVL
                     RFFAYFVSALPLYLGFLWAAFDRRKQGWHDKIARTVVINSEE"
     gene            4316..5014
                     /locus_tag="VFR57_02730"
     CDS             4316..5014
                     /locus_tag="VFR57_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEU0258320.1"
                     /translation="MIDLYTWPTPNGHKVHIMLEETGTPYRVIPVDIGAGDQFKPEFL
                     KISPNNKMPAMVDPDGPEGKGKPFALAESGAMLFYLASKTGKFLPTGLRERWQVMQWV
                     MFQMGHIGPMLGQAHHFLGYAPEKIEYAMNRYKNEANRLYGVVERRLRESRYIACDQY
                     TIADMAIVPWLRAPERQGVNIEEYPTLKRWRDAILERPAVQRALQVLAERRRTQMTPE
                     QRENLFGAAQYAKR"
     gene            4986..>5138
                     /locus_tag="VFR57_02735"
     CDS             4986..>5138
                     /locus_tag="VFR57_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258321.1"
                     /translation="MAPPNTLKDKVVWITGGGSGIGLAGGIELARAGAHVVISGRSAE
                     TLKQAEQ"
BASE COUNT          958 a         1621 c         1718 g          841 t
ORIGIN      
        1 cgctgccgaa tggggcccga aaaacgtgcg ggtgaacgcc atcgcgccgg ggctggtccg
       61 gaccgaattc gcgcgcgccc tgtgggagga cgagaagcgg cgcgccgacc gcatcgcggc
      121 cacgccgttg cgccgcctgg gagagccgcg cgacatcggc ggcatcgcgg tgtttctcgc
      181 gtcgccggcg gcggcattta tcaccggtca atgcatcgtc gccgacggcg gcgttactat
      241 tctctgatgg acctgaacta ttccgccgag gagctcgcat tccgcgacga cgttcgcgcc
      301 tggctcgccg ccaacctgcc acaggagctg aaggaccgcg gccacctctc caaggaagag
      361 ctgatgcgct ggcatcgcat cctggccaag aaaggctggg ttgctccggc gtggcccaag
      421 gaatggggcg gcaccggctg gaacgtggtg caacgctaca tcttcgagga agagctcggc
      481 tatgccggag ccccgccgat cattccgttc ggcatcaaca tgtgcgcgcc ggtgctgctc
      541 gcgttcggca ccgaggcgca gaagaagcgc ttcctgccgc gcatctacaa cggcgacgat
      601 ttctggtgcc aggggtactc cgagccgggc tccggcagcg atctcgcttc gctcaagaca
      661 aaggcagaga aaaaagagaa ccattacgtc gtcaacggcc agaagacctg gacctcctac
      721 gcccactacg ccgactggat cttctgcctg gtgcggaccg atccgacagt cgagaagcgc
      781 caggaaggga tctcgttcct tctcatggat atgcgctcgc ccggaatcac cgtgcgccct
      841 ctcatcctga tggacggtgc ccacgcggtg aacgaggtct tcttcgacga cgtgaaagtc
      901 ccggtcgaaa acctcgtgca caaggaaaac cagggctgga cggtggcgaa gtacctgctc
      961 ggctacgagc gcatgaatac cggccggatc ggcgaatcga aacgggagct gcggaagctg
     1021 aaagagaatt tttcctcgaa attaagtgac gtccgctttc gcgatcgcct gacgcgcgtg
     1081 gaggtcgagc tgatggcgct cgagatcacg aacctgcgct tcctggaccg catgcgccgt
     1141 accggccagc cgccgggcgc ggacgtatcg atgctgaaga tcaaaggcac ggagatccag
     1201 caggcgctca ccgagctcgc gatacaggct gccgatccgg cggcgcgcga gacccaggcg
     1261 ctcgagcggc gctacctcga catgcgcaaa acgacgatct acgcgggctc taatgaaatc
     1321 cagaggaaca tcatcgccaa gatgacgttg ggtttatgaa cttcgactac aacgaagagc
     1381 agcagcttct cgccgactcg gtacgccgct acctgcagaa ggactacgac ttcgaggcac
     1441 gcaagaagat cctcgccacg cccgagggct ggagcgagcg cgtgtgggcg acgttcgccg
     1501 agatggggct caccgggctg ccgctgtcgc cggactacgg cggtttcggc ggcggcgcgg
     1561 tcgacctgat gagcgtgatg gaagcgttcg gcgaggcact ggtggtcgag ccttacctgc
     1621 cgacaatcct cgccgccctg gcaatcgcca aaggcggcga cgcgaaacag aagcaggaat
     1681 tccttccgct cgtggtgcag ggcaagatgc ggcttgcgct cgcgaccggg ccgcgcggcg
     1741 tgatcggcgc gccgatggcc gaccggatcg tgttcggatc ggacggcagg ctgcggatcg
     1801 tggcgcgcga cgcgatcgaa gtgcgggcgt atcgcacgct cgacgacctc gcggcgggcg
     1861 acatccgctt cgacggcaag ggcgagccgc tcgccggcgg cgtgctcgac gaggtgctcg
     1921 atttcggcgc cgcgctttcg tgcgccgagg cggtcggggc gatgaagttc gcgtgcgaca
     1981 ccacgctcga gtacctgaag acgcgccgcc agttcggcgt gccgatcggc gccttccagg
     2041 cgctccagca ccgcatggtg gacatgtaca tctcctacga gcaggcgaag tccatggcct
     2101 gtcttgcatg tagcaaagca gacagcgccc tggatgcgag agagcgtgca agagccgttt
     2161 ccgcggccag gatcaagatc gcggatgccg cgcgccacat cagccaggag tcggtgcagc
     2221 tgcacggcgg catgggcatg agcgacgagc tcaaggtcag ccacaccttc cggcgcctca
     2281 ccgcgctcgg ccgccgcttc ggcgacgtgg accagcacct cgcgcgcttt gcctcgcttt
     2341 agccggtggc tcgcagcggc ggtcttcgtt gccgcctgcg cgcagcagca gcccgctcca
     2401 agagacgaac ccaagccgcc gccgcaggcg ctgccgcctt tgccggcgac gctcgtggat
     2461 gagcccaatt gcccgggctg cctggcggtc acgctcacgc tccgccccga cggagcctat
     2521 ctgctacgcg atcagctcgg cgcctcggag ttctatgatt tcgggcgctg gcgctacgcc
     2581 gacggggttc tcgagctcgc gggcgatcgc gatacgcgcc gctacccgct cgagaagctc
     2641 aagaaaagct cgcaggtcga gcagctgcgc ggcccgtttc gcatggtggg cgtctatgac
     2701 ggggcgacgt tcaaggagtg ccgcaccggg ctcgcctgga ggctcgccga cacgcgcgcg
     2761 gcccaggcat tgaaaggcga agtagtgaaa gcgcaaggaa acccggtgct ggtcgcgctg
     2821 gacgcgcagt tcgaaggctc gcccgaaatg ctgcaggtct tccgcccggc cgcggtgctc
     2881 gatgcgcgcg gctgcccaag ctagactcta gcgcatggaa aaccagcgca tcgtgctcgc
     2941 gagccacccc aagggttggg tcacgcccga gaattttcga atcgagaaag cgcaggtgcc
     3001 ggcgcccgca gacggagagg tcctcgtcaa gaatctatgg ctctcgctcg atccctacat
     3061 gcgcgggcgc atcagcgagg cgaagagcta tgtgaagggc gtggacatcg gcgaggtgat
     3121 ggtcggccag accgtagggg aagtcatcga ctccagaaac aggaatctca agatcggaga
     3181 tcacgtactc acccagacgg ggtggcagtt gtatggagtg acgaaagaag cgaccaaagt
     3241 cgatgcgtcg cgcgcgccgc tcagctatta ccagggcgtg ctcggcatgc caggcatgac
     3301 ggcgtacttc ggcctgaagg acatcgggca gccgaagccc gcagagacag tggtcgtttc
     3361 ggccgcctcg ggcgcggtcg gcagcgtggt cggacagctc gcgaagctct ggggctgccg
     3421 cgcgatcggc atcgccggcg ggcgcgagaa gtgcgactac gtgacgcgcg agctcggctt
     3481 cgacgcgtgc atcgactata agtcggccaa cctgcgcgac gagctgaaag acgtggacgt
     3541 ctacttcgac aacgtgggcg gcgagatgct cgacattctg ctcgcgcgca tgaagctttt
     3601 cggccgcatc gtggtgtgcg ggatgatcgc cgactacaac gcgaccgagc cctaccgcct
     3661 gaagaatctg cgcgcggtgc tggtgaaccg gctcaagctc cagggcatga tcgtgttcga
     3721 ctggaaggag cgctacggcg aagcgctgaa ggcgctcggc ggctactacg ccgccggcaa
     3781 gctcaagacg cgcgaatcga tcgtcgaagg cctggcgaac gcgccgcagg ggctggtcgc
     3841 gctgctcaag ggcggaaatt ttggcaagca gctggtcaag ctcgcctgac tacgccggct
     3901 tctggcagcg cgccgcggcc ctggtcatcg actggctgat cgtgaccgtg atcgcggtgc
     3961 cgatcatggt gctcggcttc ggcattccgc agctctcgct caatccggcc gagcattcgt
     4021 gggacctgct ctcgtttgtc gccgtggccg ttgccgtgat cggcttctgg cgctactgcg
     4081 gcgcgacgcc cgggaaaatc gcggtcgggt tgaagatcgt cgatgcggcg accggcgctc
     4141 cgccaacgct cgggcggctg gtgctgcgct tcttcgccta tttcgtctcc gcgctcccgc
     4201 tttacctcgg cttcctgtgg gcggcgttcg atcggcgcaa gcaaggctgg cacgataaga
     4261 tcgcccgaac ggtcgtcatt aattcagagg aatagaggga aaaggagcat tagacgtgat
     4321 cgatctgtat acgtggccca ctcccaacgg ccacaaggtc cacatcatgc tggaagaaac
     4381 cggcacgccc tatcgcgtga tcccggtgga catcggcgcg ggcgaccagt tcaagcccga
     4441 gtttctcaag atctcgccca acaacaagat gccggcaatg gtcgatcccg atggccccga
     4501 gggcaagggc aagcctttcg cgctcgccga gtcgggcgcg atgctttttt acctcgcctc
     4561 gaagaccggc aagttcctgc cgacggggct gcgcgagcgc tggcaagtga tgcagtgggt
     4621 gatgttccag atggggcaca tcggcccgat gctcggccag gcgcaccact tcctcggcta
     4681 cgcgccggag aagatcgagt acgcgatgaa ccgctacaag aacgaggcga accgccttta
     4741 cggcgtggtc gagcgcaggt tgcgcgagag caggtacatc gcgtgcgacc aatacaccat
     4801 cgccgacatg gcgatcgtgc cttggctgcg cgcccccgag cgccagggcg tgaacatcga
     4861 ggagtacccg acgctcaagc gctggcgcga cgcgatcctc gagcgccctg ccgtgcagcg
     4921 cgctctgcag gtgctcgccg agcggcgacg tacccaaatg accccggagc aacgcgagaa
     4981 tctctttggc gccgcccaat acgctaaaag ataaagttgt ttggattaca ggcgggggct
     5041 cggggatcgg gctcgccggc ggcatcgagc tcgcgcgcgc gggtgctcat gtggttattt
     5101 ccgggcgaag cgcagagacg ctcaagcaag ccgaacag
//
LOCUS       DASTPD010000075         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_446399, whole genome shotgun sequence.
ACCESSION   DASTPD010000075 DASTPD010000000
VERSION     DASTPD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446399"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFR57_02740"
     CDS             <1..261
                     /locus_tag="VFR57_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEU0258322.1"
                     /translation="VRHWNIEPAHAVLAYLWSWLENQVMAAVKAVPLGQTAGQKILVE
                     LGSCLENLVETELQEDCWTNFTPGLAALSARHETQYSRLFRS"
     gene            258..881
                     /gene="ureG"
                     /locus_tag="VFR57_02745"
     CDS             258..881
                     /gene="ureG"
                     /locus_tag="VFR57_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEU0258323.1"
                     /translation="MTLRVGIGGPVGSGKTALTLALCRALRSRYEIAVVTNDIYTEED
                     AQFLVRNEALAPERIIGVETGGCPHTAIREDASINLEAVERLVRRFPGLDAVFVESGG
                     DNLAATFSPELSDLTLYVIDVAAGDKIPRKGGPGITKSDLLVINKIDLAPMVGASLEV
                     MGRDARKMRGERPFVFTNLKSGQGLDAVIRFIERQGMLQAEKKPGGG"
     gene            complement(942..1184)
                     /locus_tag="VFR57_02750"
     CDS             complement(942..1184)
                     /locus_tag="VFR57_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0258324.1"
                     /translation="MEQGKLAFTAVYLKSSHGYVGFIEELPGVHSHGRTLPEARETLR
                     KLAEVMFDEERRESEELIAGKDVVRESFFLPIPRQT"
     gene            complement(1203..1703)
                     /locus_tag="VFR57_02755"
     CDS             complement(1203..1703)
                     /locus_tag="VFR57_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0258325.1"
                     /translation="MLQKDGFFRNRRDEPFRQNQPAAPTTTTTPASTAPAPSTAPEQK
                     ADQRKESKLVVGPDIKMKGVEITDCDTLVVEGRVEATLDSRVLEIAEHGVFQGTIAVD
                     QAEIRGRFEGEITVRKMLVIQGTGKVSGKIRYARIKVEEGAELAGDLAVMDKSAAALG
                     KRVANA"
     gene            complement(1899..3569)
                     /locus_tag="VFR57_02760"
     CDS             complement(1899..3569)
                     /locus_tag="VFR57_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0258326.1"
                     /translation="MFDLVIRDALLVDGLGSPPRRAGVAVKDGRIVEVGKILDGARET
                     IVADGLALMPGIIDNHTHYDAQLTWDPWASPSPALGVTTAIIGNCGFTIAPCRARDRD
                     LVMKNLTQVEGMSLEVLRTGIRWDFETVPQYLDMLERRGAALNVAAFVGHSSIRTYVM
                     GDAATERPATDEEISQMRALVLQGMHAGAIGFSTSTSPAHNGEGGRPMPSRLADAAEL
                     RALVGCLREAGRGVFMLTKGGQTAIGFLEELAAQSQRPVVIAALFHSSTNPEAVFQDL
                     QKIQEANERGRRLLGAVSCCPLAMDFTLHSPYVFEGLDAWRPALALKGERFKAKLREA
                     QFRDALRAELSKPAHFRLFNGEWQRVHVIQSTRAGLEQRSVAELAVAAGADPLDFMLD
                     LALEEKLDTVFSALLLNADEAAVGRMLKHPCSLVSLSDAGAHLTFFNDAGFGLHLLGH
                     WVRERGVLTLAEAAWRLCGQPAQLFGVKERGALKPGCHADLLLFDPAVVDRGPKRRLF
                     DLPGGHPRLTTSALGVHGVWVNGVRVAGKDGPCDAKELPGRVLREFSA"
     gene            3594..4277
                     /locus_tag="VFR57_02765"
     CDS             3594..4277
                     /locus_tag="VFR57_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0258327.1"
                     /translation="MFDLKDKTAVVTGGAKGIGAATAKLLREAGARVEVLDLSTGCDV
                     TDEAQVNNAFAGIGGLDILVNNAGRAIRKRAVEVAKEDWDAVIELNLTALFLCSRLAH
                     PHMKKRGGGAIVNLASIMGLSGGIYPNASYQASKGGVVNLTRALALEWASDNIRVNAV
                     APTYVRTDFTMPIFSNPDVLKTVMAHTPLGRFPEPEDVAAAILFLASNAARCITGVTL
                     PVDSGYLAR"
     gene            complement(4312..>4498)
                     /locus_tag="VFR57_02770"
     CDS             complement(4312..>4498)
                     /locus_tag="VFR57_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0258328.1"
                     /translation="QAWRTFSLFNPLVYLISGFRWSFYGTEDVPVGVSLAVTAAFLLM
                     FLGIVAWIFKTGYRLKT"
BASE COUNT          769 a         1482 c         1482 g          765 t
ORIGIN      
        1 gtgcgccact ggaacatcga gccggcccac gcggtgctcg cttatctctg gtcctggctc
       61 gaaaaccagg tgatggcggc ggtgaaggcg gtgccgctcg ggcagaccgc cggccagaag
      121 attctcgtcg aactcgggtc gtgcctcgag aatttggtcg aaaccgaatt gcaggaagat
      181 tgctggacca acttcacacc cgggctcgcg gctctgtcag ccaggcacga aacccagtac
      241 tcgaggctct tccgttcatg acccttcgcg tgggcatcgg cggcccggtc ggctcgggca
      301 agacggcgct gacgctcgcg ctgtgccgcg cgctgcgcag ccgctacgaa atcgcggtgg
      361 tgacgaacga catctacacc gaggaggacg cgcaattcct ggtgcgcaac gaagcgcttg
      421 cgcccgagcg catcattggc gtcgagaccg gcggctgccc gcataccgcg attcgcgagg
      481 acgcctcgat caacctggag gcggtagagc gcctggtgcg ccgctttccc gggctcgacg
      541 cggtgttcgt cgagtctggc ggcgacaatc tcgccgcaac cttcagcccg gaattgtccg
      601 acttgacgct ctacgtgatc gacgtggccg cgggcgacaa gatcccgcgc aagggcgggc
      661 cgggcattac gaagtcggac ctgctggtga tcaacaagat cgacctcgcg cccatggtcg
      721 gagcgtcgct cgaggtcatg ggccgcgacg cgaggaaaat gcgcggcgag cggccgttcg
      781 tcttcaccaa cctgaagagc ggccagggcc tcgacgccgt gatccggttc atcgagcgcc
      841 agggcatgct gcaggccgaa aaaaagcccg ggggcggata gctccgcccc gggccggact
      901 agcacctcct tcagggtagg aggcgcgaga gagaaaccgg tctacgtctg gcgcgggatg
      961 gggaggaaga aggactcgcg cacgacgtcc ttgccggcga tcagttcttc ggattcacgc
     1021 cgctcttcgt cgaacatcac ctcggcgagc ttgcgcagcg tctcgcgcgc ctcgggcagc
     1081 gtgcgcccat gggaatgcac cccaggcagc tcttcgatga agccgacgta gccgtggctc
     1141 gacttgagat acaccgccgt aaacgcgagc tttccttgtt ccattcgcgc gctcgagcgg
     1201 ctctacgcgt tagcgacgcg cttgcccagg gcggccgcag acttgtccat cacggcgagg
     1261 tcgccagcga gctcggcgcc ttcctcgacc ttgatccggg cgtagcggat cttgcccgag
     1321 accttgccgg tgccctgtat cacgagcatc ttgcgaaccg taatctcgcc ttcgaagcgg
     1381 ccgcggatct cggcctggtc gacggcgatg gtgccttgaa acacgccgtg ctcggcgatc
     1441 tctaggacgc gcgagtcaag ggtcgcttcg acgcgcccct cgacgaccag cgtgtcgcag
     1501 tcggtgattt cgacgccctt catcttgatg tcgggaccga caaccagctt ggattccttg
     1561 cgctgatcgg ctttctgctc cggcgcggtg gagggcgcgg gtgccgtgct cgcgggcgtt
     1621 gtcgttgtcg tcggcgcggc cggttgattc tggcggaatg gctcgtcgcg cctgtttcgg
     1681 aagaacccgt ctttctgtag cacttgaact cccctctctg tagtagtggg taggacaacc
     1741 aatgctcgta tgcacgatcc atacctgcat gcaagccttt gaaagcatag cacttaaaat
     1801 gcaagcgctg tgtcgaggct gtcgcctgac ggcgacgtcg gggcccggtg acgtctaaag
     1861 gcttttgttt aaacagggtt tttcttgtcg ccgaagggct acgcagaaaa ctctcgcagc
     1921 actcggcccg gaagctcctt cgcgtcgcag ggcccgtcct tgccggcgac ccggacgccg
     1981 ttgacccaga cgccgtgtac gccgagcgcg gatgtagtga gacgcgggtg gccgcccggc
     2041 aggtcgaaca ggcgccgctt cggcccgcgg tccaccaccg ccggatcgaa gagcagcagg
     2101 tcggcatggc agccgggctt gagcgcgccg cgctccttaa cgccgaaaag ctgcgctggc
     2161 tgcccgcaca ggcgccacgc cgcctcggct agcgtcagca cgccgcgctc gcgcacccaa
     2221 tggccgagaa ggtgcaggcc gaagccggcg tcgttgaaga atgtgagatg ggcgccggcg
     2281 tccgacagcg agaccaggct gcacggatgc ttgagcatgc ggccgaccgc cgcttcgtcg
     2341 gcgttgagca gcagcgcgct gaagaccgtg tcgagcttct cctcgagcgc gagatccagc
     2401 atgaagtcga gcgggtctgc gcccgccgcg acggcgagct cggccaccga tcgctgctcg
     2461 agcccggcgc gcgtcgactg gatgacgtgc acccgctgcc attcgccgtt gaacagccgg
     2521 aagtgcgccg gcttcgacag ttccgcgcgc agcgcgtcgc ggaattgcgc ctcgcgcagc
     2581 ttggccttga agcgctctcc cttcaacgcc agcgccggcc gccaagcatc cagaccctcg
     2641 aatacgtagg gcgaatgcag cgtgaagtcc atggcgagcg ggcagcacga aaccgcaccg
     2701 agcagtcgcc ggccgcgctc gttcgcttct tggatcttct gcagatcctg gaatacggcc
     2761 tcggggttcg tgctgctgtg aaagagcgcg gcaatcacca ccgggcgctg cgactgggcg
     2821 gcgagctcct cgaggaagcc gatcgccgtc tgcccgcctt tcgtcagcat gaatacgccg
     2881 cgcccggcct cgcgcaagca gccgaccagc gcgcgcagct cggcggcgtc ggcgaggcgc
     2941 gagggcatcg gcctgccacc ttcgccgttg tgcgccggag aggtgctggt ggagaagccg
     3001 atggcgccgg cgtgcatgcc ctgcagcacg agagcccgca tttgcgaaat ctcttcgtcc
     3061 gtcgccgggc gttcggtggc tgcatcgccc attacatacg tgcggatcga cgaatggccg
     3121 acgaatgccg ccacgttgag ggcagctccc cggcgctcga gcatgtcgag gtactgcggc
     3181 acggtctcga agtcccaccg tattccggtg cgcagcactt ccagcgacat accctcgacc
     3241 tgggtgagat tcttcatcac caggtcacgg tcgcgcgcgc ggcagggcgc gatggtgaac
     3301 ccgcagttgc cgatgatcgc ggtggtgacg ccgagcgcgg gcgaaggcga cgcccacgga
     3361 tcccacgtga gctgtgcgtc gtagtgggta tggttgtcga tgatcccggg catgagggca
     3421 aggccgtcgg ctacgatggt ttctcttgca ccatccaaga tcttgcccac ttcaacgatg
     3481 cgtccatcct tcaccgcgac cccggcgcgg cgcggcggcg agcccagccc gtcgacgagc
     3541 agcgcgtccc ggatcaccag gtcgaacatc gcgcctcagt atacttcgcg aaagtgtttg
     3601 atctgaagga caagaccgcc gtggtgacag gcggcgcgaa gggcatcggc gccgcgaccg
     3661 cgaagctcct gcgcgaggcg ggcgcgcgcg tcgaggtgct cgatctctcg accggctgcg
     3721 acgtcaccga cgaggcccag gtcaataacg ccttcgcggg tatcggcggc ctggacatcc
     3781 tggtgaacaa tgccggccgc gcgatccgca agagggcagt ggaagtggcg aaggaggact
     3841 gggacgcggt catcgagctg aacctcaccg cgcttttcct gtgctcgcgg ctcgcgcatc
     3901 ctcacatgaa gaagcgcggc ggcggtgcga tcgtcaacct cgcctcgatc atgggccttt
     3961 ccggcggcat ctaccccaac gcctcgtacc aggcgtcgaa gggcggggtg gttaacctca
     4021 cgcgcgcgct cgcgctcgaa tgggcgagcg acaacatccg cgtcaacgcg gtggctccaa
     4081 cctacgtgcg caccgacttc accatgccca tcttctcgaa tccggacgtc ctgaagaccg
     4141 tgatggcgca cacgccgctc ggccggttcc ccgagcccga ggacgtcgcg gcggcgatcc
     4201 tgttcctcgc gagcaacgcg gcgcgctgca tcaccggcgt cacgctgccg gtggactccg
     4261 gctacctcgc gcgctaaagc gagcgtccgt cgaagtgctg tattccctcc ttcaggtctt
     4321 cagcctgtag ccggtcttga agatccacgc gacaattccc aggaacatga gaaggaacgc
     4381 ggccgtgacg gcgaggctca cgccgaccgg cacgtcctcg gtgccgtaga agctccagcg
     4441 aaacccgctg atgagataca cgagcgggtt gaacaggctg aaggtgcgcc aagcctgc
//
LOCUS       DASTPD010000076         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_242765, whole genome shotgun sequence.
ACCESSION   DASTPD010000076 DASTPD010000000
VERSION     DASTPD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242765"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..438)
                     /locus_tag="VFR57_02775"
     CDS             complement(19..438)
                     /locus_tag="VFR57_02775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU0258329.1"
                     /translation="MSDCVFCRIVARQIPATVVHEDQHTLAFMDLGQVNPGHVLVALK
                     AHVENIFGLQPGQASAVANAMLRVSCAIRQAFAPHGLSVYQANGKAAGQTVFHYHVHI
                     VPRYEADGMTLTWPVKNPPREKLEEYAAKIRASLSPG"
     gene            complement(435..1253)
                     /locus_tag="VFR57_02780"
     CDS             complement(435..1253)
                     /locus_tag="VFR57_02780"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEU0258330.1"
                     /translation="MATYAIGDVQGCYDELQALLGKVSFDGSADRLWFVGDLVNRGPK
                     SLEVLRLVKRLADRAVVVLGNHDLHLVAQHEGIERAHAGDTFGDVLESREAAELVGWL
                     RRRPMMHAEGSYAMVHAGLLPQWSIAKGLSLAREVERALEGPGYRDFLEHMYGAKPHR
                     WDDALTGWDRLRIIVNAMTRMRFCDPAGRMDLKGKGTQPRPGFLRWYEARMQHDTHTL
                     LFGHWSQLGLLLHGKIVGLDSGCVWGGALSALRLEDRTLYQVACPGYQTPGGDA"
     gene            complement(1286..2785)
                     /locus_tag="VFR57_02785"
     CDS             complement(1286..2785)
                     /locus_tag="VFR57_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU0258331.1"
                     /translation="MTVATVASRALAGIDAPEVSVEVHLGPGLPAFHIVGLPDAEVRE
                     ARDRVRAALNHAHFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQLAPHALA
                     GQEFAGELSLTGELRAIRGALAMALSARAARRAFVLPEGNAPQAALAEGARILPARTL
                     LDVVAHLAGEAPLEPYSGEARCSVGTYADFSEVKGQQQAKRALEVAAAGGHSVLMVGP
                     PGTGKSMLAARFPGILPELSEAEALEIAAIHSVSANGFETVRWGERPYRAPHHSASAA
                     ALVGGGMLPRPGEISLAHHGVLFLDELPEFDRDVLEALREPLETGRVSIARAARQAQF
                     PARFQLVAAMNPCPCGHCGDRSGRCRCTPERIARYRGRVSGPLADRIDIKLEVPPPRE
                     SELVAPVAGEATTSIKQRVEAARRAQLDRQRKPNALLGTREIDNHCATDREGDQLLRH
                     ALARLLLSARAYHRVLRVARTIADLAQKSSIGADHIAEAIQYRRLDASF"
     gene            complement(2838..3077)
                     /locus_tag="VFR57_02790"
     CDS             complement(2838..3077)
                     /locus_tag="VFR57_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEU0258332.1"
                     /translation="MIDSKLFDELRSRIDDALRNSPTQDIEKNLRALLAAWFDRLDLV
                     LREDFEVQKKLLERAQAKLAELEARIAELESSKPR"
     gene            3266..4081
                     /locus_tag="VFR57_02795"
     CDS             3266..4081
                     /locus_tag="VFR57_02795"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEU0258333.1"
                     /translation="MRKSALTLSIAAALSVPVLAAAESPSPVTGNMTIASDYRFRGIS
                     QTYLGPTIQGGIDYSHPSGIYLGNWNSNVASQQFPLGSGIEMDFYGGWKTTFGDIGLD
                     IGTIYYYYPRAEWNSTIGSTTGDANFNNWEVYVGASWKWLSAKFFYALSDYFGLADEQ
                     AGSWITHKDTGVPLGARGGSDGTWYLDLSASFPVNKQLSVVAHFGMLEVEKYSELDYK
                     DWKIGITYDLSGWILGAAYVDSDADSAYYYTGGSDGVKETGKSTVVLSVMKTF"
     gene            4097..4435
                     /gene="glnK"
                     /locus_tag="VFR57_02800"
     CDS             4097..4435
                     /gene="glnK"
                     /locus_tag="VFR57_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0258334.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAVKSELLEQAIEAIEKSANTGKIGDGKIFVFDLEKVIRI
                     RTGETDAAAL"
     gene            4446..>5671
                     /locus_tag="VFR57_02805"
     CDS             4446..>5671
                     /locus_tag="VFR57_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0258335.1"
                     /translation="MKKFCAFLALVLSLVFAGATLAQDKPAAAPDKPAAAEVKKEEAK
                     PAPPTPNKGDVSWMLVSTALVLMMSVPALALFYGGMVRSKNALSMLMQVFVVFSLVTV
                     LWCIYGYSLAFTEGNAYFGGFDRLFLKGAMDPGKGEFANAATFSKGVVIPEIVFVVFQ
                     ATFAAITCCLILGAFAERAKFSAVLVFITLWFTFSYAPIAHMVWFWMGPDAYTSAKVV
                     DEMNSKAGLIWQWGALDFAGGTVVHINAGIAGLVGAYLVGKRIGFGKEAMPAHNLPLT
                     MIGASLLWMGWFGFNAGSALEAGASASLAFLNTYLATACAVLSWSVGEALFKGKPTML
                     GAASGAVAGLVAITPAAGNVGLPGAFVIGLAAGLVCLWGVSGLKKLLGADDSLDVFGV
                     HAVGGILGALLTGVFNS"
BASE COUNT          991 a         1799 c         1864 g         1017 t
ORIGIN      
        1 cgcggcgcgt tcggctattc accctggcga caatgacgcc ctgatcttgg ccgcgtactc
       61 ttcgagcttc tcgcgcggcg ggttctttac cggccaggtg agcgtcatgc cgtcggcttc
      121 gtagcgtgga acgatgtgga cgtggtaatg gaacaccgtt tgcccagccg ctttaccgtt
      181 cgcctggtac accgagagcc cgtgcggcgc gaaggcttgt cgaatagcgc acgagacccg
      241 cagcattgcg ttggcgacgg ctgacgcctg accgggctgc aatccgaaga tgttttcgac
      301 gtgcgccttc agggccacaa gtacgtgccc cgggttcacc tggccgaggt ccatgaaggc
      361 gagggtgtgc tgatcctcgt gcactacggt ggccgggatc tgccgtgcca cgatcctgca
      421 gaacacgcaa tcgctcacgc gtcgcctcct ggagtctggt agcccgggca tgctacctga
      481 tagagggtgc ggtcttcgag ccgcagcgcg ctgagcgcgc cgccccacac gcatccgcta
      541 tccaggccga cgattttgcc gtggaggaga agtccaagct gcgaccagtg gccgaagagc
      601 agcgtgtggg tgtcgtgctg catgcgggcc tcgtaccagc gcaagaagcc gggccgtggc
      661 tgcgtaccct tgcccttcag atccatgcgg cccgcggggt cgcagaagcg catccgcgtc
      721 atggcattta cgatgattcg cagccggtcc cagcccgtaa gcgcgtcgtc ccatcggtgg
      781 ggcttggctc cgtacatatg ctcgagaaaa tcccggtagc cgggaccttc gagcgcgcgc
      841 tcgacttcgc gtgcaagcga gagccccttc gcgatgctcc actgcggcag caagccggca
      901 tggaccatgg cgtaggaacc ctcggcgtgc atcatgggcc gccgccgcag ccagccgacc
      961 agctcagccg cctcgcggga ctccagcacg tcaccgaagg tatcgcctgc gtgcgcgcgc
     1021 tcgatgcctt cgtgctgcgc gaccagatgc aggtcgtggt tgccgagtac gaccaccgcg
     1081 cgatccgcga gcctcttcac caagcgcaag acctcgagcg atttgggccc gcggttgacc
     1141 agatcgccga cgaaccacag ccgatcggca gagccgtcga atgagacctt gccgagcagc
     1201 gcctgcaact cgtcgtagca gccctgcacg tcgccgatcg cgtacgtcgc catgcggagt
     1261 cactatagcg cgggtgccgc tcgcctcaga agcttgcgtc gagccggcgg tattggatcg
     1321 cttcggcgat gtggtcggca ccgatcgagc ttttttgcgc aagatcggcg atggtgcgtg
     1381 caacgcgcaa cacgcggtga tatgcgcgtg ccgagagaag caggcgcgcg agcgcgtgac
     1441 gaagtagctg atcgccttcg cggtcggtcg cgcaatggtt gtcgatctcg cgcgtgccga
     1501 gcagcgcatt cggcttgcgc tggcgatcga gctgggcacg gcgtgcggct tcgacccttt
     1561 gctttattga ggttgtcgcc tcgcccgcca ccggcgcgac cagctcgctc tcgcgcggcg
     1621 gcgggacttc cagcttgatg tcgatgcggt ccgcaagcgg cccggacacg cgaccgcggt
     1681 agcgcgcgat acgctcgggc gtacagcggc agcggccact gcggtcgccg caatgcccgc
     1741 atgggcaagg gttcatcgcc gcaacgagtt ggaagcgggc cgggaactgc gcctggcgcg
     1801 ccgcgcgcgc gatcgacacc cggcctgttt cgagcggctc gcgcagcgcc tccagtacgt
     1861 cacggtcgaa ctccgggagc tcgtcgagga acagcacgcc gtggtgggcg agggagatct
     1921 cccccgggcg gggcagcatg ccgccgccga cgagcgctgc cgcagaagcg ctgtgatgcg
     1981 gcgcgcggta gggccgctcg ccccagcgca ccgtctcgaa accgttcgcc gacaccgagt
     2041 gaatcgcggc gatttcgagc gcctctgcct ccgaaagctc gggcaggatg cccgggaagc
     2101 gcgcggcaag catcgacttg ccggtacccg gcggcccgac catcagcacg ctgtggccgc
     2161 cggccgcggc gacctcgagc gcccgcttcg cctgttgctg ccccttcact tcgctgaaat
     2221 cggcgtacgt gccgacggaa caacgcgctt cgccgctgta cggttcaagc ggcgcttcgc
     2281 cagcaagatg cgccacgacg tcgagcagcg ttcgcgctgg cagaatccgg gcgccctcgg
     2341 cgagcgcagc ctgcggagcg ttgccctccg gcaggacgaa ggcgcgccgc gctgcgcgcg
     2401 ctgaaagcgc catggcgagc gcacccctta tcgcccgcag ctccccggtc agcgaaagct
     2461 cgccggcgaa ctcctggcca gcgagcgcgt gcggcgcgag ctgaccgctc gcggcgagaa
     2521 ttccaagtgc aatcggcagg tcgaagcggc tggagtcctt aggcaactct gcgggtgcga
     2581 ggttgaccgt gatacggcgc gcggggaatt cgaagtgtgc gtggttgagc gcggcgcgca
     2641 cgcgatcgcg cgcctcgcgc acctccgcat cgggcagccc gacgatgtga aaggcgggca
     2701 ggccggggcc gaggtgaacc tcgacgctta cttcgggcgc atcaataccc gcgagcgcgc
     2761 ggctcgcaac cgtagcgacc gtcatggctt ccctctcact gactaggaag aaggaacgct
     2821 gatggcgccg cgccggttta ccgcggcttg ctcgattcga gctcggcgat gcgcgcttcg
     2881 agctcggcga gcttcgcctg cgcgcgctcg agcagcttct tctgtacctc gaaatcctcg
     2941 cgcagcacga ggtcaaggcg gtcgaaccac gcggcgagga gcgcgcgaag gtttttctct
     3001 atgtcttgcg tcggcgagtt gcgcaacgca tcgtcgatgc ggctgcgcag ctcgtcgaag
     3061 agtttggagt cgatcaccta tcgaaaggta ccccaagcgc tgcgcaccaa tggcgagcaa
     3121 atcaattctg cgcatgcacc aatatggtgc tgcagcagat tcagggtcat gcaagtagct
     3181 gaatatacga gtaattgtgc aatggcatgc gagatgctaa gaggcgacgt catctcacat
     3241 ctttcatttc aagggagaag cctcgatgcg caagtccgct ctcacgttgt ccatagctgc
     3301 agcgctttcg gtcccggtgc tggctgccgc cgaatcgccc tcccccgtaa ccggaaacat
     3361 gacgatcgcg agcgattacc gcttccgcgg gatctcgcag acctacctcg ggcccacaat
     3421 ccaaggcggc atcgattact cgcacccgag cggcatctac ctcggaaact ggaactccaa
     3481 cgtcgccagc cagcagtttc cgctcggctc cggtatcgag atggacttct acggcgggtg
     3541 gaaaaccacg ttcggcgata tcggcctcga catcggcacg atctactact actacccgcg
     3601 ggcggagtgg aacagcacga tcggtagcac gaccggcgat gccaacttca acaattggga
     3661 ggtctacgtc ggcgctagct ggaaatggct ttcggcgaag ttcttctacg ccctgagcga
     3721 ctatttcggc ctcgccgacg agcaggcggg cagctggatc acgcacaagg ataccggcgt
     3781 gccgctcggc gcacggggcg gctccgacgg cacctggtat ctggatctca gcgcgagttt
     3841 cccggtgaac aagcagcttt cggtcgtggc gcacttcggc atgctggagg tcgagaagta
     3901 cagcgagctc gattacaagg actggaagat cggcatcacc tatgacctga gcggctggat
     3961 cctcggtgca gcctacgtcg attcggacgc cgatagcgcg tactactaca ccggcggcag
     4021 cgacggcgta aaagagaccg gcaagagcac cgttgtgctc tcagtaatga agacattctg
     4081 aacccgcgga ggagccatga aactggttac ggcaatcatc aagccgttca agctcgacga
     4141 ggtgcgcgag gcgctgtccg ccatcggcgt gcagggcatt acggtcaccg aggtcaaagg
     4201 cttcgggcgc cagaaggggc ataccgagct ctaccgcggc gccgagtacg tcgtcgattt
     4261 ccttcccaag gtgaaggtgg aagccgcggt caagtcggag ctgctcgagc aggcgatcga
     4321 ggcgatcgag aagtcggcga acaccggcaa gatcggcgac ggcaagattt tcgtgttcga
     4381 tcttgagaaa gtcattcgca tccgcaccgg cgagaccgat gcggcggcac tctgaggaga
     4441 acccgatgaa gaaattctgc gcattcctgg ccctagtgct ctcgctcgtc ttcgcgggcg
     4501 cgacgctggc gcaagacaag ccggcagcag cgcccgacaa gccggccgcg gccgaagtga
     4561 agaaggagga agcgaagccg gctcccccga cgcccaataa gggtgatgtc agctggatgc
     4621 tggtctcgac cgcgctggtc ctgatgatga gcgtgcccgc gctcgcgctt ttttacggcg
     4681 gcatggttcg ctcgaagaac gcgctatcga tgctgatgca ggtattcgtg gtgttttcgc
     4741 tggtcaccgt gctgtggtgc atatacggct acagcctggc gttcacggaa ggcaacgcgt
     4801 acttcggcgg cttcgatcgc ctgttcctca aaggtgccat ggatcctggc aaaggcgaat
     4861 ttgccaatgc cgccaccttt tcgaagggtg tcgtcatccc cgagatcgtg ttcgtggtgt
     4921 tccaggcgac cttcgccgcg atcacctgct gcctgatcct cggcgccttc gcggagcgcg
     4981 ccaagttctc cgccgtactg gtattcatca cgctgtggtt caccttcagc tatgcgccga
     5041 tagcgcacat ggtgtggttc tggatgggcc cggacgcgta caccagcgcg aaagttgtcg
     5101 acgagatgaa cagcaaggcc ggcctgattt ggcagtgggg ggcgctcgat ttcgcgggcg
     5161 gcacggtggt gcacatcaac gccggtatcg ccggcctggt gggcgcctac ctggtcggca
     5221 agcgtatcgg gtttggcaag gaagcgatgc cggcgcacaa cctgccgctt acgatgatcg
     5281 gcgcgtctct gctctggatg ggatggttcg gcttcaatgc cggctcggcg ctagaagcgg
     5341 gcgcatcggc atcgcttgca ttcctgaaca cgtatctcgc caccgcatgc gcggttctct
     5401 cgtggagcgt tggcgaggcg ttgttcaagg gcaaacccac gatgctcggc gcagcctccg
     5461 gcgccgtcgc cggcctggtc gccatcacac cggcggcggg taacgtcggg ctgccaggcg
     5521 cgttcgtgat cggcctcgcg gctggactcg tctgcctctg gggcgtaagc ggcctgaaga
     5581 agctgctggg tgccgacgat tcgctcgatg tgttcggcgt gcacgcggtc ggcgggattc
     5641 tcggggcatt gctcaccggc gtattcaact c
//
LOCUS       DASTPD010000077         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1059729, whole genome shotgun sequence.
ACCESSION   DASTPD010000077 DASTPD010000000
VERSION     DASTPD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059729"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFR57_02810"
     CDS             complement(<1..304)
                     /locus_tag="VFR57_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0258336.1"
                     /translation="MDKLDAAAIALPGAETRVVERCTSTNDLLLKEQIGERPVLLAAE
                     EQTAGRGRRGRRWHSTRAAGITFSLGRRVRRPARELAAISLVAGVAVARALHALGVG"
     gene            complement(314..1918)
                     /locus_tag="VFR57_02815"
     CDS             complement(314..1918)
                     /locus_tag="VFR57_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258337.1"
                     /translation="MNVLEVRNLTIKLPQGGDRPEAVSGVSFEVQKREILCLVGESGS
                     GKSVIAQAIMGLLPKTLPVAQGTISLEGENLLAAPASRLRALRGARMSMVFQEPMTAL
                     NPVMRCGTQIDEVLSHHTNDSAAIRLQKTVSLLREVALPDPERILASYPHQLSGGQRQ
                     RIMIAMALALEPALLIADEPTTALDVTTQAQILKLLSELQQSHGMGVLFITHDFGVVA
                     EIAHRVAVLREGRLVELGAKLEVLQQPRHEYTKMLIRSVPTLEPHERPADNSAPVVLE
                     TRDLSKTYVDRGWLGARREVHAASGVSIELRKGQTLGIVGESGSGKSTVARCVLRLVE
                     PSAGEIRLGGRDIASLGTRALRPLRSKVQIVFQDPYRSLNPRRNVGEAIIEGPLNYGV
                     PRARALERARRLLELVRMDPGTLERYPHQFSGGQRQRICIARALALEPDLLVADEPVS
                     ALDVSVQAQVLALLAEIRSRLELAMLFITHDLRVAAQVCDRVAVMQQGRVVEYGPARQ
                     VFLEPRHEYTRALFAAAPGRGFAFGG"
     gene            complement(1915..2772)
                     /locus_tag="VFR57_02820"
     CDS             complement(1915..2772)
                     /locus_tag="VFR57_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258338.1"
                     /translation="MLLKNWSVRIGGSVLVLLIGIAVLAPWLGTVDPTLFDAASRDLK
                     PGAAGEIMTLEGEVKRHTFLMGSDSFGRDVYSRVLYGTRVSLVVGIASALLAVAFGVL
                     CGLVAGYLRWLDGLLMRVMDGIMAIPAILIAIAVVALWRASLPTVIIAIAIPEIPRVT
                     RLVRALVLSIREEPYVEAAIALGTPTRKIMFGHILPNTLAPLLVQGTYVCAFSILLEA
                     ILSFLGVGLPPDIPTWGNIMAEGRVQFVAYPHNVFFPGIFLALTVLAVNILGDGLRDT
                     LDPKMAKRV"
     gene            complement(2772..3713)
                     /locus_tag="VFR57_02825"
     CDS             complement(2772..3713)
                     /locus_tag="VFR57_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258339.1"
                     /translation="MVKFIARRLAATVPVLLVVAVFVFLLLRLTPGDPAAIMAGDAAS
                     AEQIEQIRAGLGLDQPIVVQFGIWLGKVLSGDLGESFYFRIKVATLIGQRLEPTFALA
                     TLTILLAVIVAVPLGAIAAWRFGGWFDRALMGFSVLGFSIPVFVLAYLLIWLVSLKLG
                     WLPVQGYVRMSDGFFLFLRHLILPAITLSVIYIALIARVTRASVLEALGEDYIRTARA
                     KGLPELRVLVRHALANSAVPIATVIGIGIAILIGGVVVTESVYAIPGLGRLTVDAVLA
                     RDFPTIQGVILFFSLIYVLVNLLIDLSYVFLDPRIRY"
     gene            complement(3719..5296)
                     /locus_tag="VFR57_02830"
     CDS             complement(3719..5296)
                     /locus_tag="VFR57_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258340.1"
                     /translation="MRYRIVAVLGSLVLSSLPALSLHAQDKVLRVVPHSNLNILDPIW
                     TTQYMARNHGYMVYDTLFGTDEKSRIQPQMVDKWTVSKDQKEWSFTLRAGLEFHDGKP
                     VTGEDVIASLSRWGKRDAMGQKLMTFVERMDSPKPDTFRMFLREPCGFVLEALGKPSS
                     NVPFIMPKRVADTPADKQITEAIGSGPYIFKTDEFKPGDRAVYLKNNKYVPRKEAPSG
                     TAGGKHVYVERVEWNLALRDAQSQVNALANGEVDVLERPAFESYEQLRKAPGVELVTK
                     DPLGFQYMCRFNHLHPPFNNAKVRQAALAALPQEPFLRAQVGDKDLYRPCASMFTCGT
                     PYGSTKGSEIQSKSNMKKAQELVKASGYDGTPVVIIKPTDLASIQKLPDVAAQLLRQA
                     GFKVDLQAMDWNTVVSRRAKKEPPAQGGWNIFCTAWVAPDIWNPLTNAAVGAAGEKSW
                     FGWPSDEQLEKLRDDFARATDEGKKKALAEAIQARAFEIGTHAPLGEYVNPLAARKNV
                     SGWVIGPGDIYWNVKKN"
     gene            5342..>5752
                     /locus_tag="VFR57_02835"
     CDS             5342..>5752
                     /locus_tag="VFR57_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0258341.1"
                     /translation="MARIAIGGFQHETNTFAPSKADYSAFEAGGGWPGVQYGEPIFGA
                     VEGANIPAAGAIQALRALGHSMVGTAWGAASPSAHVTSEAFERITTELVSRLKQALPV
                     DGVCLDLHGAMVVETYDDGEGEILRRVREAVGPRV"
BASE COUNT         1021 a         1875 c         1914 g          942 t
ORIGIN      
        1 cgccgacgcc gagggcatgc agcgcgcgcg ccaccgcgac cccggcaacg agcgagattg
       61 cggcgagctc gcgcgctggc cggcgcacgc gccggccaag cgagaacgta atgccggccg
      121 cgcgcgtgct gtgccagcgc cgcccgcgcc gcccgcgtcc agcggtctgc tcctccgcgg
      181 ccaacagcac cggcctctcg ccgatctgtt ctttcagcaa cagatcgttg gtcgaggtgc
      241 agcgctcgac gacccgcgtc tctgcgcccg gcagagctat ggcggcggcg tcgagcttat
      301 ccatgctcct agtttagccg ccaaaggcga acccccgccc cggtgcggcg gcgaaaagcg
      361 cccgcgtgta ttcatggcgt ggctcaagga acacttggcg cgccggcccg tactccacaa
      421 cccgtccttg ctgcatgacc gccacccggt cgcacacctg cgcggccacg cgaaggtcgt
      481 gcgtgatgaa aagcatcgcc agctcgaggc gcgagcgaat ctccgccagc agggcaagca
      541 cctgcgcctg cacggaaaca tcgagcgccg acaccggctc gtcggccacg aggaggtccg
      601 gctcgagcgc cagggcgcgc gcaatgcaga tgcgctggcg ttgcccgccg ctgaactggt
      661 gcggatagcg ctcgagcgtc cccgggtcca tgcgcacgag ctcgagcagg cgccgcgcgc
      721 gctcgagcgc ccgcgcgcgc ggcactccgt agttgagcgg tccctcgatg atcgcctcgc
      781 cgacgttgcg tcgcgggttg agcgagcggt agggatcctg gaacacgatt tgcaccttgg
      841 agcgaagcgg cctcagcgcg cgcgtgccga gggatgcaat gtcccggccg ccgagccgga
      901 tctcgcccgc cgacggctcc accagccgaa gcacgcagcg cgccaccgtg gacttgcccg
      961 agcccgactc gccgacgatg ccgagcgtct ggcccttgcg cagctcgatg cttactccgc
     1021 ttgccgcgtg gacttcgcgc cgagcgccga gccagccgcg gtcgacgtag gtcttggaaa
     1081 gatccctcgt ttccagcacg actggggcgg agttgtccgc cggtcgctcg tgcggctcca
     1141 gtgttggaac ggagcggatc agcatcttcg tgtactcgtg gcgcggctgt tgcagcacct
     1201 cgagcttggc tccgagctcc accagccggc cttcgcgcag cacggcaacg cggtgggcaa
     1261 tctccgccac cacgccgaaa tcatgcgtga tgaagagcac ccccatgccg tggctttgct
     1321 gcaattcgga gagcagcttc agaatctgcg cctgcgtcgt gacgtcgagc gccgtggtcg
     1381 gctcgtcggc aatgagcagc gccggctcga gcgcgagcgc catcgcgatc atgatgcgct
     1441 ggcgctgccc cccggaaagc tggtgcgggt acgaggcgag gatgcgctcg gggtcgggca
     1501 gcgcgacctc gcggagcagg cttacagttt tttggaggcg gatggccgct gagtcgttcg
     1561 tatgatggct cagcacttca tcgatttgag tcccgcagcg catgaccggg ttgagcgcgg
     1621 tcatcggttc ttggaagacc atcgacatgc gtgcgccgcg aagcgcgcgc agccggctgg
     1681 ccggcgcagc gagcaggttc tcgccctcga gcgagatggt gccctgggcg acgggaagcg
     1741 tcttcggcag caatcccatg atcgcctgtg cgatcacgct cttgcccgag ccggactctc
     1801 cgaccaggca caggatttcc cgcttctgca cctcgaacga gaccccgctg actgcctcag
     1861 gacggtcccc tccctggggc agcttgatgg tcaggttgcg gacttcgagc acgttcatac
     1921 gcgcttcgcc atcttcgggt cgagcgtgtc gcgcaagccg tcgccgagga tgttcaccgc
     1981 gagcaccgtg agcgcgagga agatgccggg gaagaagaca ttgtgcgggt aagcgacgaa
     2041 ctgcacgcgg ccttccgcca tgatgtttcc ccaagtcggg atgtcgggcg ggaggcccac
     2101 gccgagaaag gacagaatcg cctcgagcag gatggagaac gcgcaaacgt atgtcccctg
     2161 cacgagaagc ggcgcgagcg tgttcggaag gatatggccg aacatgatct tgcgcgtcgg
     2221 ggtgcccagg gcgatggccg cctcgacgta gggctcctcc cggattgaca gcacgagcgc
     2281 gcgcacgagg cgcgtgacgc gcgggatctc cgggatcgcg atggcgatga tcaccgtggg
     2341 caggcttgcg cgccacagcg ccactacggc aatcgcgatc aggatggccg ggatcgccat
     2401 gatgccatcc atcacgcgca tcagcaggcc gtcgagccag cgcaggtatc ccgcgacgag
     2461 gccgcacagc acgccgaaag ccaccgcgag cagcgccgag gcgatgccga cgaccagcga
     2521 cacacgcgtg ccgtagagca cgcggctgta gacatcccgg ccgaaggagt cggatcccat
     2581 aagaaaagtg tgccgcttga cttccccctc gagcgtcatg atctcgcccg cggcgcccgg
     2641 cttgagatcg cggctcgccg cgtcgaacaa ggtcgggtcg accgtaccga gccagggagc
     2701 gaggactgca atgccgatca acaagaccaa tacggacccg ccgatgcgca ccgaccagtt
     2761 cttgagcagc atcagtaacg gatcctgggg tcgagaaaca cgtaggagag gtcgataagc
     2821 agattcacca atacatatat gagggagaaa aagaggatca ccccctggat ggtggggaag
     2881 tcacgcgcca gcactgcgtc cacggtgagg cgtccaaggc cggggatggc gtagaccgac
     2941 tcggtcacca ctacgccgcc gatcaggatc gcgatgccga tgccgatcac cgtcgcgatc
     3001 ggcaccgcgg aattggcgag cgcgtggcgc accagcacgc gcagctcggg cagccccttg
     3061 gcgcgcgcgg tgcggatgta gtcctcgccg agcgcctcga gcaccgatgc gcgcgtgacg
     3121 cgcgcgatca gcgcgatgta gatcaccgaa agcgtgatcg ccggcaggat caggtgccgc
     3181 aagaaaagga agaagccgtc ggacatccgc acatagccct gcaccggcag ccacccgagc
     3241 ttgagcgata cgagccagat cagcaggtag gcaagcacga acacagggat cgagaaaccg
     3301 agcaccgaga aacccatcag cgcgcggtcg aaccagccgc cgaagcgcca tgcggcgatc
     3361 gctcccaggg gtaccgcaac gatgactgca agaagaatgg tcaacgtggc gagcgcgaat
     3421 gtgggctcga ggcgctggcc gataagcgtc gcgaccttga tgcggaaata gaacgactct
     3481 ccgaggtcgc cggaaaggac tttgccgagc cagataccga actgcaccac gatcggctgg
     3541 tccagcccga gcccggcgcg aatttgctcg atttgctccg cgctcgcggc gtcgcccgcc
     3601 atgatcgccg cggggtcgcc cggcgtcaga cgcaggagca ggaagacgaa gaccgcgacg
     3661 accagcagga ccggaacggt cgctgccagc cgccgcgcga tgaacttaac cagtcagatc
     3721 agttcttctt gacgttccag tagatgtcgc ccgggccgat aacccagccg ctcacgttct
     3781 tgcgtgcggc gagcgggttg acgtattcgc cgagcggggc gtgcgtgccg atctcgaacg
     3841 cgcgcgcctg aatcgcctcg gcgagcgcct tcttcttgcc ttcgtcggta gcgcgcgcaa
     3901 agtcgtcacg cagcttctcc aactgctcgt cgctcggcca gccgaaccac gacttctcgc
     3961 ccgctgcgcc gaccgcggcg ttggtgagcg ggttccagat gtcgggcgcg acccacgcgg
     4021 tgcagaagat gttccagccc ccttgcgccg gcggctcctt cttcgcgcgc cggctcacca
     4081 cggtgttcca gtccatcgcc tgcaggtcga ccttgaaacc ggcttggcgc agaagctggg
     4141 ccgcgacgtc gggcagcttc tggatcgagg caaggtcggt gggcttgatg atcaccaccg
     4201 gggtgccgtc gtagcccgag gccttcacga gctcctgcgc cttcttcatg ttggacttcg
     4261 actggatctc gctgcccttc gtgcttccat agggcgtgcc gcaggtgaac atcgaggcgc
     4321 aaggccgata gagatccttg tctccgacct gggcgcgaag gaacggctcc tggggcagcg
     4381 cggcgagcgc cgcctgtctc accttggcgt tgttgaacgg cggatgaagg tggttgaagc
     4441 ggcacatgta ctggaagccg agcggatcct tggtgacgag ctcgaccccg ggcgctttcc
     4501 tcagctgctc gtagctttcg aaggcggggc gctccagcac gtcgacctcg ccgttcgcaa
     4561 gcgcgttaac ctgcgactgc gcgtcgcgca gcgccaggtt ccattccaca cgctcgacgt
     4621 agacgtgctt gccgcccgcg gtgccggacg gagcttcctt ccgcggaaca tatttgttgt
     4681 ttttcagata gacagctctg tcgccgggct tgaattcgtc ggtcttgaag atgtagggcc
     4741 cgctgccgat cgcctcggtg atctgtttgt ccgcgggcgt gtcggcgacg cgcttgggca
     4801 tgatgaacgg tacgttggat gagggcttgc cgagcgcttc cagcacgaag ccgcagggct
     4861 cgcgcaggaa catgcggaac gtgtcgggtt tgggggaatc catgcgttcg acgaacgtca
     4921 tgagcttctg gcccatcgcg tcccgcttgc cccagcgcga gagcgatgcg atcacgtcct
     4981 cgcctgtgac cggcttgccg tcgtgaaact caagcccggc gcgcagcgtg aagctccact
     5041 ccttctggtc tttgctcacg gtccatttgt cgaccatctg cggctggatc cggctcttct
     5101 cgtcggtgcc gaagagcgtg tcgtagacca tgtagccgtg gttacgcgcc atgtactgcg
     5161 tggtccagat cggatcgagg atgttcaggt tcgagtgcgg cacgacacgc aacaccttgt
     5221 cctgcgcgtg cagcgataaa gccggaagcg aagagagaac gagcgatccg aggacggcga
     5281 caatgcgata acgcatgcga gaatcctcct cctctaaggg agaaacgagg ttagcaggtt
     5341 catggcgcgc atcgcaatcg gcggcttcca gcacgagacc aacacgttcg cgccttccaa
     5401 ggccgattac tccgcgtttg aggcaggcgg cggctggccc ggcgtgcagt acggcgagcc
     5461 catcttcggt gcggtcgagg gcgcgaacat tcccgcggcc ggtgccatcc aggcgctacg
     5521 cgcgctcggc cacagcatgg tcggcactgc ctggggcgcg gcgagcccgt ctgcgcacgt
     5581 gacctcggag gcgtttgaaa ggattactac cgagcttgtg tcgagattga agcaagccct
     5641 tcccgtcgac ggcgtctgtc tcgacctgca cggcgccatg gtggtcgaga cttacgacga
     5701 cggcgagggg gagatcctgc gccgtgtgcg cgaggcggtc ggcccgcgag tc
//
LOCUS       DASTPD010000078         6838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_243217, whole genome shotgun sequence.
ACCESSION   DASTPD010000078 DASTPD010000000
VERSION     DASTPD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243217"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /gene="glnA"
                     /locus_tag="VFR57_02840"
     CDS             <1..432
                     /gene="glnA"
                     /locus_tag="VFR57_02840"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU0258342.1"
                     /translation="FEAPINLAYSARNRSAACRIPYVSSPNGRRVEVRFPDPTTNAYL
                     AFAAMLMAGLDGVQNKIHPGDPIDKDMYHLPPEEAAKVPHVCASLEEALGCLRDDHAF
                     LTRGGVFSEDFLESYVELKEQELTRFRMTTHPVEFDMYYSS"
     gene            500..976
                     /locus_tag="VFR57_02845"
     CDS             500..976
                     /locus_tag="VFR57_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEU0258343.1"
                     /translation="MHRAWAFGVLLVGLPAMNAHAQQGVSEVFKCRNEQGQITYTNDR
                     RQAEKQKCELVTRQINVAPSRPPAARTPEFPRETPQASASARERQRKVLESELAAEQE
                     ALRQAQQALAEQEAVRFGDERNYARVLERLQPYKDTIQNHEKNIQALQRELANLKN"
     gene            977..2002
                     /gene="glnL"
                     /locus_tag="VFR57_02850"
     CDS             977..2002
                     /gene="glnL"
                     /locus_tag="VFR57_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEU0258344.1"
                     /translation="MAASFAGLDLLATAVVVLDDEFTVRFANPAAENLLTTGARSLIG
                     QPFLGFFAERADLERSLEEARVIHWDYSARNVTYVRTGRQPVPLSCTVTRIDAFGMAL
                     LAELRPISEQLRIEREARIESEQQANRELIRNLAHEIKNPLGGLRGSAQLLERELERP
                     ELREYTQVIIKEADRLQTLMDRMLTPHRAPRLEPLGMHEVLERVRSLLRAEFGVEIVR
                     DYDPSLPDIMGDREQLIQAVLNVARNAAQAGSKNVTFRTRVLRQVTILKQRYKLALEL
                     QVIDDGPGVPEEIQDRIFNPLVSGREGGSGLGLSLAQTFVHYHHGVIEFESRPGRTIF
                     RILLPIR"
     gene            1999..3432
                     /gene="ntrC"
                     /locus_tag="VFR57_02855"
     CDS             1999..3432
                     /gene="ntrC"
                     /locus_tag="VFR57_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEU0258345.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIAYNSFSSAQEALDALAGA
                     MPEVLVSDIRMPGRSGLELLQAVKQRYPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDVDQAVDLIRRALEERRRDTQVNEPLPETEILGQAPAMQEVFRAIGRLSQSSATVL
                     ITGESGTGKELVARALHRHSARAQQPFIAINTAAMPKDLLESELFGHERGSFTGAQQQ
                     RRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQQIKVNVRVIAATH
                     QNLEQRVREGTFREDLYHRLNVIRLRLPSLRERAEDIPLLAKHFLARSAQQLGVEPKR
                     IADEALAHISRLEFPGNVRQLENLCHWLTVMAPGQVIDLANLPPEFRTQVPGDAAAGA
                     GDWIASLEQEAERRLARGDTGILDVLGRQFERALIIKALARTGGRRIDAANLLGMGRN
                     TITRKIQELGIEDEKSGSEPDFPGQER"
     gene            complement(3461..4516)
                     /locus_tag="VFR57_02860"
     CDS             complement(3461..4516)
                     /locus_tag="VFR57_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258346.1"
                     /translation="MKPYNILILGASYGSLLATKLLLAGHNVKLVCLAAEADAINSGG
                     TRVRMPVKGHQGLVEVHSWKLPGKLSAEGPGTIKPSEYDLVVLAMQEPQYRSAGVREL
                     LDAVAKAKVPCMSIMNMPPPPYLARIPGISTKELRPCYTDASVWDSFDPALMTLCSPD
                     PQAFRPPEEPVNVLQVRLPTNFKSARFPSDAHTAILKKLEADIEASRFDKMELPVKLK
                     VHESVFVPLAKWAMLIAGNYRCVTKEGPRAIRDAVHSDINASRATYAWVVNLCLLLGA
                     KESDLVPFDKYAAAAQTLGSPSSAARALYGGAPYIERVDRLVQAIAAQKDLRLPALDE
                     LVGLVDAKLEANRKKAA"
     gene            4621..6201
                     /gene="aceB"
                     /locus_tag="VFR57_02865"
     CDS             4621..6201
                     /gene="aceB"
                     /locus_tag="VFR57_02865"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU0258347.1"
                     /translation="MSGPLPQGIGISGPIGKEFAQILSPEAMGFVAKLHRKFELRRQE
                     LLVRRAQRQKEFDAGAKPDLLKETQSIRDSEWSVASQPKDMLDRRVEITGPTDRKMVI
                     NALNSGASTFMADFEDANCPTWHNMIDGQINLRDAVRRTITFEQNGKQYRLNDKTAVL
                     IPRPRGWHLDEKHVTVDGKPVSGGIFDFALFFFHNAKELIKRGSGPYFYLPKMESHTE
                     ARLWNDIFVFSQSELGIPEGTVRATALIETVVAAFEMDEILYELRNHSAGLNIGRWDY
                     IFSCIKKFRANKDFCLADRAQVTMTAPFMRAYALLLVKTCHRRGAPAMGGMAAQIPIK
                     NDPAANEAAIEKVRQDKLREVTDGCDGTWVAHPGLVPVAKQVFDQHMPQPNQYTRQRA
                     DVNVTAKQLLDFQPERPITEAGLRNNISVGIQYLGAWLAGNGCVPVYNLMEDAATAEI
                     SRSQIWQWIRSPKGVLEDGRKVTAELFKKLLADELAKVKNAGAEGQLDEAARLFERIT
                     TDDQYVEFLTLPAYELID"
     gene            6326..6568
                     /locus_tag="VFR57_02870"
     CDS             6326..6568
                     /locus_tag="VFR57_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258348.1"
                     /translation="MNTDMAARALAALDEIRGLLQAAQFAAYKFEQMGLQQSLETLSD
                     HIHRVKVAADDTRARIQRSVRRSAAAQLPRLDSNEA"
BASE COUNT         1338 a         2210 c         2138 g         1152 t
ORIGIN      
        1 ttcgaggcgc cgatcaatct cgcctactcg gcgcgcaatc gctctgccgc ctgccgaatc
       61 ccgtatgtat cgagccccaa cggccgccgc gtggaggtgc gcttccccga cccgaccacc
      121 aacgcctacc tcgcgttcgc cgcgatgctg atggcgggcc tggacggagt gcagaacaag
      181 atccatcccg gcgacccgat cgacaaggac atgtaccacc tgccgcccga agaagccgcc
      241 aaggtgccgc acgtgtgcgc ttcgctcgag gaggcgctcg gctgcctgcg cgacgaccac
      301 gcgttcctca cgcgcggtgg cgtgttctcg gaggacttcc tcgagtcgta tgtcgagctg
      361 aaggagcagg agctcacccg cttccgcatg acgacgcatc ccgtcgagtt cgacatgtat
      421 tactcgtctt gaggaattcc caccctgacc ccaaacggcc ggcggagcaa gtttcttttt
      481 gctttgatag acttagctca tgcaccgcgc ctgggcgttt ggggtcctgc tcgttggcct
      541 gccggccatg aatgcgcacg cccagcaagg cgtcagcgag gtcttcaaat gccgcaacga
      601 gcaggggcag atcacgtaca ccaacgaccg gcgccaggcg gaaaagcaga agtgcgagtt
      661 ggtcacgagg cagatcaacg tcgcgccgtc gcggccgcca gccgcccgca ccccggaatt
      721 tccgcgcgag acgccgcaag cgagcgcgag cgcgcgcgag cgccagcgca aggtgcttga
      781 aagcgaactc gccgccgagc aggaggcgct gaggcaggca cagcaggcgc ttgccgagca
      841 ggaagccgtg cgcttcggcg acgagcgcaa ctacgcccgt gtccttgagc gactgcaacc
      901 gtacaaggac acgatccaga atcacgagaa gaacatccaa gcgcttcagc gcgagctcgc
      961 gaacctgaag aactaagtgg ccgcctcgtt cgccggcctc gacttgctgg caacggcagt
     1021 ggtggtgctc gacgacgaat tcacggtgcg cttcgccaac ccggcggccg agaacctgct
     1081 gactacgggc gcgcgcagcc tgatcgggca gcccttcctc ggtttcttcg cagagcgcgc
     1141 ggacctcgag cgctcgcttg aggaggcgcg cgtcatccat tgggattact cggcgcgcaa
     1201 cgtcacctac gtgcgcaccg gccgccagcc ggtgccgctc tcttgcaccg taacgcgaat
     1261 cgacgctttc ggcatggcgc tgctcgcgga gctgcgcccg atcagcgagc agcttcgcat
     1321 cgagcgcgag gcgcgcatcg aatcggagca gcaagcgaac cgcgagctca tccgcaacct
     1381 cgcgcacgag atcaagaatc ctcttggcgg gctgcgcggc tcggcacagc ttctcgagcg
     1441 tgagctcgaa cgtccggagc tgcgcgaata cacgcaggtc attatcaagg aggccgaccg
     1501 gctgcaaacg ctgatggacc gcatgctcac accgcaccgc gcccctcggc tcgaaccgct
     1561 cgggatgcac gaggtgctcg agcgcgtgcg cagcctcctg cgagccgaat tcggcgttga
     1621 gatcgtgcgc gactacgacc cgagcctgcc cgacatcatg ggcgaccgcg agcagctcat
     1681 ccaggcggtc ctcaacgtcg cgcgcaacgc cgcgcaggcc ggcagcaaga acgtaacgtt
     1741 caggacgcga gtcctccggc aagtcaccat cctcaagcag cgctacaagc tggcattaga
     1801 attgcaagtg atcgacgacg gcccgggcgt gccggaggag atccaggatc ggatcttcaa
     1861 tccgcttgtt tcgggcaggg aaggtggctc cggcctagga ctctcgctcg ctcagacgtt
     1921 cgtgcactac caccacggcg tcatcgagtt cgagtcgcgg ccgggccgaa cgatattcag
     1981 gatcctgctg ccgataagat gaagccggtc tggatcgtcg acgacgaccg ttccatccgc
     2041 tgggtgatcg agaaggcgct cagccgggaa ggcatcgcct acaactcatt ctcgtccgcc
     2101 caggaggcgc tcgacgcgct cgcgggcgcg atgcccgagg tgctcgtctc cgacatccgt
     2161 atgccggggc gctcgggtct cgagcttctg caagcggtaa agcagcgcta tcccgcggtg
     2221 cctgtcatcg tgatgacggc gtactccgac ctcgacagtg ccgtcgcagc gttccaaggc
     2281 ggcgcatacg agtatctgcc caagcctttc gacgtcgacc aggcggtcga cctcatccgg
     2341 cgcgcgctcg aggagagaag acgcgacacc caggtaaatg aacctcttcc ggaaacggaa
     2401 attcttggcc aggcgcccgc catgcaggaa gtcttccggg ccatcggccg gctttcgcaa
     2461 tcgagtgcga ccgtcctcat taccggcgag tcgggaaccg ggaaggagct cgtcgcgcgc
     2521 gccctgcacc gccatagtgc gcgtgcacag cagccgttca tcgccatcaa caccgccgcg
     2581 atgccgaagg atctcctcga gtccgagctc ttcggccacg aacgcggctc gttcaccgga
     2641 gctcagcagc agcgtcgcgg ccgcttcgag caggccgagg gcgggacgct tttcctcgac
     2701 gaaatcggcg acatgcctgc cgagctgcag acgcggcttt tgcgtgtgct ttccgatggt
     2761 acgttctatc gggtcggcgg ccaccagcag atcaaggtca acgttcgcgt gatcgcggcc
     2821 acgcaccaga acctcgagca gcgcgtgcgc gaaggaacgt tccgcgagga cctctaccac
     2881 cgcctcaacg tcatccgcct gcggctgccg agcctgcgtg agcgcgccga ggacatcccg
     2941 ctattggcga agcatttcct cgcgcgcagc gcgcagcagc tcggcgtcga gcccaagcgc
     3001 atcgccgacg aagcgctggc gcatatctcg cgcctcgagt ttccgggcaa cgtgcgccag
     3061 ctcgagaacc tttgccactg gcttaccgtg atggcgccgg ggcaggtgat cgatctcgcc
     3121 aacctgccgc ccgagttccg cacccaggtg cctggcgacg ccgcggccgg tgccggcgac
     3181 tggattgcgt cgctcgagca ggaagccgag cgtcgcctcg cgcgcggcga caccggcatc
     3241 ctcgatgtcc ttggccgtca gttcgagcgc gcgctcatca tcaaggccct tgcgcgcacc
     3301 ggcggccgcc gcatcgacgc cgcgaatcta ttgggcatgg gcagaaatac gattacgcgc
     3361 aaaatccagg aactcggcat cgaggacgaa aaatcggggt cagagcccga tttcccaggg
     3421 caggagcggt gataccggaa atcgggctct gacccgattc ttaagcggct ttcttccggt
     3481 tcgcctcgag tttcgcgtcg accaggccga cgagttcatc aagcgcaggg aggcggaggt
     3541 ccttttgcgc ggcgatggcc tgtaccaggc ggtcgacgcg ctcgatgtaa ggcgcgccgc
     3601 cgtagagcgc gcgcgcggct gacgacgggc tgcccagggt ctgcgccgcg gcggcgtact
     3661 tgtcgaacgg gacgaggtcg ctttccttcg cgccgagcag caagcagagg ttcaccaccc
     3721 aggcatacgt ggcgcgcgaa gcgttgatgt cggagtggac ggcgtccctg atcgcgcgcg
     3781 ggccttcctt cgtgacgcag cggtagttgc cggcgattag catggcccac ttcgcgagcg
     3841 gcacgaaaac cgattcgtgc accttgagct tgaccggcag ctccatcttg tcgaaacgcg
     3901 acgcttcgat gtcggcttcg agctttttca ggatcgcggt atgcgcgtcg gagggaaagc
     3961 gcgccgactt gaagttggtc ggcagcctga cctgcagcac gttgaccggc tcttccggcg
     4021 ggcggaacgc ctgcggatcg gggctgcaaa gcgtcatcag cgccggatcg aagctgtccc
     4081 agacgctcgc atcggtatag cagggcctga gctctttggt cgaaatgcca ggtatccgcg
     4141 cgaggtaggg aggcggcggc atgttcatga tcgacatgca gggcactttg gccttggcga
     4201 cggcgtcgag cagctcgcgc acgccggccg agcgatactg cggctcctgc atcgcgagca
     4261 cgacaaggtc gtattccgag ggcttgatcg ttcccggtcc ttcggccgag agcttcccgg
     4321 ggagtttcca cgagtgcacc tccaccagac cttgatgccc tttgaccggc atgcgcaccc
     4381 gcgtgccacc gctgttgatc gcgtcggcct cggcggccag gcagacgagc ttgacattgt
     4441 gtccggcaag aaggagcttc gtcgcgagca gggagccgta cgaggctccc aggatcagaa
     4501 tgttgtacgg tttcatgatg tgaaatccgc cgtcggtact tgaaagatca ggctactccg
     4561 ttcggcgcat gaattcatgt tgcactgcca catttcactt aatagaatgc tgcaatgcac
     4621 atgtcaggcc cgcttccaca aggaatcggt atctccggcc caatcggcaa ggagttcgct
     4681 cagatcctga gtccggaagc catgggcttt gttgccaagc tccatcgaaa gttcgaattg
     4741 cgccgccagg aactgctggt gcgtcgcgcg cagcggcaaa aagaattcga cgccggggcc
     4801 aagcccgact tgctgaaaga aacacagtcc attcgggact ccgaatggag cgtggcgtca
     4861 cagccgaaag acatgctcga ccggcgcgtc gagatcactg gcccgaccga ccgcaagatg
     4921 gtgatcaacg cgctgaactc gggcgcctcc acgttcatgg cggacttcga ggacgccaac
     4981 tgcccgacgt ggcacaacat gatcgacggg cagatcaatc tgcgcgacgc ggtgcggcgc
     5041 acgatcacgt tcgagcagaa tggcaagcag taccggctga acgataagac cgcggtcctc
     5101 atcccgcggc cgcgcggctg gcacctcgac gagaagcacg tcaccgtgga cggcaagccg
     5161 gtttcgggtg gcatcttcga cttcgcgctc ttcttcttcc ataacgcgaa agagctaatc
     5221 aagcgcggct cgggaccgta cttctacctc ccgaagatgg agtcgcacac cgaggcgagg
     5281 ctgtggaacg acatcttcgt cttctcgcaa agcgaactgg gcattcccga gggcacggtg
     5341 cgcgccacgg cgctcatcga aaccgtggtt gccgcgttcg agatggacga gatcctctac
     5401 gagctgcgca accactccgc cggcctcaac atcgggcgtt gggactacat cttctcgtgc
     5461 atcaagaaat tccgtgccaa caaggatttc tgcctcgccg accgcgcgca ggtcacgatg
     5521 actgcgccgt tcatgcgcgc atacgcgctg ctccttgtga aaacctgtca ccggcgcggc
     5581 gcgccggcca tgggcggcat ggcggcgcag attccgatca agaacgaccc cgcggcgaac
     5641 gaagccgcga tcgagaaggt gcggcaggac aagctgcgcg aagtgaccga cggctgcgac
     5701 ggtacctggg tcgcgcaccc gggcctcgtg ccagtggcga agcaggtgtt cgatcagcac
     5761 atgccgcagc ccaatcagta cacgcgtcag cgcgcggacg tgaacgtcac cgcgaaacag
     5821 ctgctcgact tccagccgga gcgcccgatc accgaggccg ggctgcgcaa caacatttcg
     5881 gtcggaatcc agtacctcgg cgcctggctc gccggcaacg gctgcgtccc ggtctacaac
     5941 ctcatggaag acgccgccac cgcggagatc tcgcgctcgc agatctggca gtggatccgc
     6001 tcgccgaaag gcgtgctgga agacggtcgc aaggtcaccg cggagctttt caagaagctc
     6061 ctcgctgatg agctcgccaa agtgaagaac gccggcgccg aaggccagct cgacgaggcc
     6121 gcccggctct tcgagcgcat caccacagac gatcagtacg tcgagttcct gactctcccc
     6181 gcctacgagc tgatcgacta gaagttcagt ctcagcttca ggccgcccgc ctcgggcatt
     6241 tccggacgcg aagataagca atgcttcgac aggcttggaa caatgcccca cttaagaatc
     6301 acaacgcaaa ggggggaatg tcatcatgaa caccgatatg gccgctcgcg ccctagcggc
     6361 ccttgacgaa atcagaggct tgctgcaagc ggcacagttc gctgcgtaca agttcgaaca
     6421 gatgggactg caacagtcct tggaaaccct gtccgatcac atccatcgcg tgaaagtcgc
     6481 agccgacgac acgcgcgccc gcatccagcg ttcggtgcgg cgaagcgcag ctgcacaact
     6541 tccgcgtctc gacagtaacg aggcgtagac cagcggcgga acagtcggca tgctgcactg
     6601 aaggacgcgc ggctgcgctt tttcggcatc ccaagtgtca aatcctgcaa gagagggcaa
     6661 aacaaagaaa tgcggcaagc ttcggcgccc ctccatgatt taagaaggga cattgcagga
     6721 gaaggtaggg gcatggggag gaatagagtt cttttttgcg ccgtagcgca cagtgaaaat
     6781 actggtagtc gaagatcagg cggtaacccg ggagctgatg atttcagtgg ctcgcgct
//
LOCUS       DASTPD010000079         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1263984, whole genome shotgun sequence.
ACCESSION   DASTPD010000079 DASTPD010000000
VERSION     DASTPD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263984"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFR57_02875"
     CDS             complement(<1..410)
                     /locus_tag="VFR57_02875"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU0258349.1"
                     /translation="MKRLFLALLCCLAPQPGIAAQIEPGEWEFTSISTSRLLPGPQKA
                     SFKRCIRKEEAENPDKWMAEPSQQGDCKVIPGKKSADTYTWTMHCPRGNLRGAGSARL
                     SSASMEGETTMTGELQGQKFEIRTRVTGKRLGPC"
     gene            complement(410..808)
                     /locus_tag="VFR57_02880"
     CDS             complement(410..808)
                     /locus_tag="VFR57_02880"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU0258350.1"
                     /translation="MRRRLCFISGLFLFASAAWADIEPGNWELTATTEMQGIKEPASF
                     TQTRCLTPEDARDPSRLFGSSPGAGCQFLNRNDTGSAFTFEIECSSPQPIRGSGNVRY
                     KRDSIEGELELKTDMFTARSRISGRRIGGC"
     gene            complement(808..2208)
                     /gene="gltX"
                     /locus_tag="VFR57_02885"
     CDS             complement(808..2208)
                     /gene="gltX"
                     /locus_tag="VFR57_02885"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0258351.1"
                     /translation="MAAAPKVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGTFVLR
                     IEDTDLERSTKESVDAILRSLEWLGIDWDEGPFFQMQRLARYKEVAEQLIGAGHAYYD
                     YMTREELELLRKQQIERGEKPRYDGRWRPERAKGKTPPANVPPVVRFKTPEEGEAGWK
                     DLVKGPISFPNAELDDLVLLRADGVPTYNFGVVVDDIDMDITHVIRGDDHVNNTPRQI
                     HIFEALKRPLPHFAHVPMILGADGERLSKRHGAVSVLQYHDEGYLPEALLNYLVRLGW
                     SHGDEEIFSRERFVEWFDLGHVSRSPAQFNPEKLAWLNQQYVKAAADARLAALVEPEL
                     RRRGAQPGGGPSLERVVALVKERASSIVQLADEAMLFYAVDVRPAPGELSADTLKALR
                     TLKARLGSVAWERSAISDTIKDVAKTSGLKMPQVAMPLRHIVTGRSQTPSIDAVLELL
                     GRDMVLTRLAQHLEAD"
     gene            complement(2211..3275)
                     /locus_tag="VFR57_02890"
     CDS             complement(2211..3275)
                     /locus_tag="VFR57_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0258352.1"
                     /translation="MLFRQLFDPQSSTYTYLLADPAFREALLIDPVFEQARRDAALVD
                     ELGLKLAWTLETHVHADHVTGAWLLREKLGSRIAVSASSSAEGADRYLKPHDKISFGK
                     RSLEARSTPGHTAGCTTYVLEDRSMAFTGDALLIRGCGRTDFQGGDARTLFHSVREQI
                     FSLPDRCLLYPGHDYRGLTVTSVGEEKLFNPRLAESIGEGDFVGYMTHLGLPHPKQID
                     VAVPANLRCGKPPTVTAADPDWAPLTYTFAGIWEVQPHWLEEHLRDVQIVDVREPDEF
                     NGPLGHVPGAVLIPLGTLQGKSEGLCKERPVVAVCRSGARSAQATVLLAKAGFDRVAN
                     LSGGMLRWRAQRYAVEGGSE"
     gene            3327..3401
                     /locus_tag="VFR57_02895"
     tRNA            3327..3401
                     /locus_tag="VFR57_02895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3359..3361,aa:Val,seq:cac)
BASE COUNT          588 a         1175 c         1204 g          666 t
ORIGIN      
        1 ttgcagggcc cgagccgttt gccggtgacg cgggtgcgga tctcaaattt ctgaccctgc
       61 agttcgcccg tcatggtcgt ctcgccttcc atggaggcgg agctcaggcg cgcgctgccc
      121 gcgccgcgga ggttcccgcg cgggcaatgc atcgtccatg tgtaggtgtc ggccgatttc
      181 ttgccgggaa tgaccttgca gtcgccctgc tggctcggct cggccatcca cttgtccggg
      241 ttctccgcct cttccttgcg gatgcagcgc ttgaagcttg ctttctgcgg cccgggaaga
      301 agccgcgagg tcgaaatgga agtgaactcc cattcgccgg gctcgatctg ggctgcgatt
      361 ccgggctgcg gagcgaggca gcaaagcagc gcgaggaaaa gccgcttcat cagcagcctc
      421 cgatccggcg cccgctgatg cgcgagcgcg cggtgaacat gtccgtcttc agttcgagct
      481 cgccctcgat cgagtcgcgc ttgtagcgca cgttgccgga gccgcggatc ggctgcggcg
      541 agctgcactc aatctcgaac gtgaacgccg atccggtatc gttgcggttg aggaactggc
      601 agcccgcgcc cggcgaggag ccgaacaaac ggcttgggtc gcgcgcgtct tcgggcgtca
      661 ggcagcgcgt ctgcgtgaag cttgccggct ccttaatccc ctgcatctcg gtggtcgcgg
      721 tgagttccca gtttccgggc tcgatatcgg cccacgccgc cgaagcgaaa aggaaaagac
      781 ccgagatgaa gcacagccgc cgtcgcatca atccgcctcc aaatgctgcg cgagccgcgt
      841 gagcaccatg tcgcggccga ggagctcgag gaccgcgtca atggacggcg tttgagagcg
      901 cccagtcacg atgtggcgca gcggcatcgc cacctgcggc atcttgaggc cgctcgtttt
      961 ggccacgtcc ttgatcgtat cgctgatcgc gctgcgctcc caggccaccg acccgagccg
     1021 cgctttcagt gtgcgcagcg ccttcaaggt atccgcactc aactcgccgg gtgcgggtct
     1081 tacgtcaacg gcgtagaaca acatcgcctc gtcggcaagc tgcacgatcg aactcgccct
     1141 ttcctttacc agcgcaacca cgcgctcgag cgacgggccg ccgccgggct gcgcgccgcg
     1201 ccggcgaagc tccggctcga ccagcgcggc gaggcgcgcg tcggctgcgg ccttgacgta
     1261 ttgctggttg agccacgcga gcttctcggg gttgaactgc gcgggcgagc gcgacacgtg
     1321 cccgaggtcg aaccactcga cgaagcgttc cctggagaag atttcctcgt cgccgtgcga
     1381 ccagccgagc cgcacgaggt aattgaggag cgcctccggc aggtagcctt cgtcgtggta
     1441 ttgcagcacg ctcaccgcgc cgtggcgctt cgacaggcgc tcgccatctg cgcccaggat
     1501 catcggcaca tgcgcgaagt gcgggagcgg tcttttcagt gcctcgaaga tatggatctg
     1561 ccgcggcgtg ttgttgacgt gatcatcgcc gcggatcacg tgggtgatgt ccatgtcgat
     1621 gtcgtcgacc accacgccga agttgtaggt cggtacacca tcggcgcgca gcagcacgag
     1681 gtcgtcgagc tcggcgttcg gaaacgagat cgggcctttg acaaggtcct tccagccggc
     1741 ttcgccctcc tccggcgttt tgaagcgaac cacgggcgga acgttcgccg gaggcgtttt
     1801 ccccttggcc ctttccgggc gccaacggcc gtcgtagcgc ggcttctcgc ccctttcaat
     1861 ctgctgcttt ctcaggagct cgagctcctc gcgcgtcatg tagtcgtagt aggcgtggcc
     1921 cgcgccaatg agctgctcgg cgacttcctt gtaacgcgcg aggcgctgca tctggaagaa
     1981 cgggccctcg tcccagtcga tgccgagcca ctcgaggctc ctcaggattg cgtcgaccga
     2041 ttccttcgtc gagcgctcca ggtccgtgtc ctcaatgcgc aggacgaacg tgccgccatg
     2101 gcggcgcgcg tacgcccacg agaaaagcgc ggtgcgcgcg ccgccgatgt gcagatagcc
     2161 ggtcgggctc ggcgcgaagc gcgtcctcac cttcggcgca gccgccatcg ctattcgctg
     2221 ccgccttcga ccgcgtagcg ctgtgcgcgc cagcgcagca tcccgcccga gaggtttgcg
     2281 acccggtcga accctgcctt ggcgaggagc accgtagcct gcgccgagcg cgcaccggag
     2341 cggcagacgg ccacgacggg cctttctttg caaagccctt cgcttttccc ctgaagggtt
     2401 cccagaggga tcaagaccgc tcccggcacg tggccgagcg ggccgttgaa ttcgtcgggc
     2461 tcgcgcacgt cgacgatctg cacgtcgcgc aggtgctctt ccagccaatg cggctgcact
     2521 tcccagatgc cggcgaaggt gtaggtaaga ggtgcccagt cgggatcggc agcggtgacg
     2581 gtgggaggtt ttccgcacct caggttggcc gggaccgcga cgtctatctg cttcggatgc
     2641 ggcaggccga ggtgcgtcat gtacccgacg aagtcgccct cgccaatcga ctcggcgagc
     2701 cgcggattga agagcttctc ctcgccgacg ctggtgacgg taagcccgcg gtagtcgtga
     2761 ccggggtaga gcaggcaacg gtccggcagc gagaaaatct gctcgcgcac gctgtggaat
     2821 agcgtgcgcg cgtcgccgcc ctggaagtcg gtgcggccgc agccgcggat gagaagcgcg
     2881 tcgccggtga acgccatcga gcggtcttcg agcacgtacg tcgtgcagcc ggcggtgtga
     2941 ccaggcgtgg agcgagcttc aagggatcgt ttcccgaagc tgatcttgtc gtgtggcttc
     3001 aagtatctgt cagcgccttc ggcgctgctg gaagcggaga cggcgatacg gctgccgagc
     3061 ttctcgcgca gcagccacgc cccggtaacg tggtcggcat ggacgtgggt ctcgagcgtc
     3121 caggcgagct tgagtccgag ctcgtcgacg agcgcggcgt cgcgccgcgc ttgctcgaac
     3181 accggatcga tcagcagcgc ctcgcgaaac gcggggtcgg cgagcagata agtataagtt
     3241 gaggactgcg gatcgaagag ctgccggaag agcatggtgg ctaactttac cgccgttttt
     3301 gctagaatcg tttttcctga cgcatcgggt gcttagctca gcggtagagc acttccttca
     3361 cacggaaggg gccagtggtt cgatcccact agcacccacc atttccggaa acctcgattc
     3421 gctttctccg gcaggctaag cccgtgccgc tgcatctggc aacctcggtc gtgcaccagc
     3481 agctctcgag ctagacggcg acgaagcaaa ggccggcgtc agaaaggcgt gagctaggtc
     3541 ggcaggcgct gcggatcgtt ccagagcggc gcgcagtggt gagccgcctc gaactcgcgc
     3601 aagagctcgc tgtgccggcg gtccggatcg tcg
//
LOCUS       DASTPD010000080         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_40037, whole genome shotgun sequence.
ACCESSION   DASTPD010000080 DASTPD010000000
VERSION     DASTPD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40037"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFR57_02900"
     CDS             <1..434
                     /locus_tag="VFR57_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0258353.1"
                     /translation="MHTHTTTGMAVACLEEGLSPHNFYGAMLVGKLAYHEFEGITVEP
                     SEKERLVRDIGDKPAVILRNHGLLAWGPSVPEAFLMLWTMQRACDVQIAASGAGAVHP
                     IRPEVFPQTVREGGPAEKRTCEDTFAALQRQIDTIDPTYRD"
     gene            436..1386
                     /locus_tag="VFR57_02905"
     CDS             436..1386
                     /locus_tag="VFR57_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258354.1"
                     /translation="MRTVLASVALAVFCSGAHAQYPNKPVRLIVPFPPGGAAEVGARI
                     FAQPLGQALGQPVLIETKPGADGAIAADATMKAAPDGYTLFYATNTAFSWVPAVRKDP
                     PYDPVNDFTPVSLVGQFGFFLFSHPSVPATNMKELFAYLRANPGKLNYGSGNSTSIVS
                     TAQLLQREKLDVVHVPYKGDGPLMVDLLGGRVHFAIATPGTAAPQVREGKLRALATLL
                     PSRSPLLPDAPTMAEAGLKELSVTPWGGVFGPKGLPRDIADRVSRELQAVLTRPEVRE
                     AFGKLAFEPRGSTPEQLAAYTREQLAVWRRAIADAGIKPE"
     gene            1441..>4042
                     /gene="icmF"
                     /locus_tag="VFR57_02910"
     CDS             1441..>4042
                     /gene="icmF"
                     /locus_tag="VFR57_02910"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEU0258355.1"
                     /translation="MTDLSDSSKLVYKARHKIRFVTAASLFDGHDASINIMRRILQAS
                     GAEVIHLGHNRSVDEVVGAALQEDVQGVAVSSYQGGHLEYFKYMLDLLRSQGGEHIKV
                     FGGGGGVIVPAEIKELQDYGIARIYSPEDGQRMGLQGMINDMLARCDFDASHKDPKTL
                     PQIITALEVGVLNNKELLEKAKSNPAPVLGITGTGGAGKSSLTDELVRRFRLDQGDAL
                     KIAVLSVDPTRRKTGGALLGDRIRMNAIHGPNVFMRSLATRDTGTELSAALGDAIAAC
                     RAEGYGLVMVETAGIGQGDAAIVPHVNASLYVMTPEFGAASQLEKIDMLDYADFVAIN
                     KFDRRGADDALRDVRKQVQRNREAFRTAPEDMPVFGTIASRFNDDGVTSLYHAVAGKL
                     GFQTTRLPRPAGKVSSAVHVIVPPKRQRYLAEIAETVRGYHHRVEQQSRLARERQQLF
                     GTRAMLGRSFAELDALIESRRLDAEAQRLLDGWPKVIRSYSGEEQIVKVRNKELRTQL
                     THTSLSGTKVPKVALPRYEDHGELLRWRMRENLPGEFPFTAGVFHFKRENEDPTRMFA
                     GEGDPFRTNKRFHLLSRDMPAKRLSTAFDSVTLYGFDPDERPDIYGKVGNSGVSIATL
                     DDMKALYAGFDLCDPSTSVSMTINGPAPTILAMFFNTAIDQQVEKFRQEKGKDPSAEE
                     LEAIKAQTLASVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIQEYFIANQVRNFYS
                     VSVSGYHIAEAGANPISQLAFTLANGFTFVEAYLARGMKIDAFAPNLSFFFSYGMDPE
                     YAVLGRVARRIWAVAMKERYRGNERSQKLKFHSQTSGRSLHAQEIAFNDIRTTLQALI
                     STYDNTNSLHT"
BASE COUNT          752 a         1351 c         1289 g          650 t
ORIGIN      
        1 taatgcacac gcacaccacg accggcatgg cggtcgcgtg cctcgaggaa ggcctctccc
       61 cgcacaactt ctacggtgca atgctcgtcg gcaagctggc gtaccacgaa ttcgagggca
      121 ttaccgtcga gcccagcgag aaggagcggc tggtgcgcga catcggggac aagccggcgg
      181 tcatcctgcg caaccacggg ctgctcgcct gggggccgag cgtgccggaa gctttcctga
      241 tgctctggac catgcagcgc gcttgcgacg ttcagatcgc agcaagcggc gcgggcgcgg
      301 tgcacccgat ccggcccgaa gtttttccgc agacggtgcg cgagggcggg ccggccgaga
      361 agcgcacctg cgaggacacg ttcgcggcgc tccagcgcca gatcgacacc atcgatccga
      421 cctaccggga ttgacatgag aacagtgctg gcgagcgtcg ctctcgctgt tttttgcagc
      481 ggcgcgcacg cgcaatatcc gaacaaaccc gtccgcctta tcgtgccatt cccgccaggc
      541 ggcgccgccg aggtcggtgc ccgcatcttc gcgcagccgc tcggtcaggc gctcggccag
      601 ccggtcctca tcgagaccaa gcccggggcc gacggtgcga tcgcggccga cgccacgatg
      661 aaggccgcgc cggacggtta cacgctcttc tacgccacta acactgcgtt ctcctgggtt
      721 ccggcggtgc gcaaggatcc gccctacgat ccggtgaacg acttcacgcc cgtctccctg
      781 gtgggtcagt tcggcttctt cctcttcagc cacccgagcg tgccggcgac gaacatgaag
      841 gagctcttcg cgtacctccg tgcgaacccc ggcaagctga actacggcag cggcaacagc
      901 acttcgatcg tctccaccgc ccaactcctg cagcgcgaga agctcgacgt ggtgcatgtg
      961 ccgtacaaag gagacggtcc cctcatggtc gacctgctcg gcggccgggt tcacttcgcc
     1021 atcgccacgc cggggaccgc ggcaccgcag gtgcgtgaag gaaaactgcg cgccctcgct
     1081 acgctgctcc cgagccgcag cccgctgctg ccggacgccc ccacgatggc agaagcgggc
     1141 ttgaaggagc tgagtgtcac gccgtggggc ggagtgttcg gccccaaagg cctgccgcgt
     1201 gacatagccg atcgcgtctc gcgcgagctc caggccgtgc tgacgcgccc cgaggtgcgc
     1261 gaggcgttcg gcaagctcgc cttcgagccg cgcggctcga ctcccgagca gctcgccgcg
     1321 tacacccggg agcagctcgc cgtctggcgc cgcgctatcg cggacgccgg gatcaagccc
     1381 gaatgagccg ggcggaaccg atgttgacgt taacgtaaac gtcaactagg ctggcgcttc
     1441 atgaccgacc tgtcggactc gagcaagctc gtctacaagg caagacacaa gatccgcttc
     1501 gtcaccgccg cgagcctgtt cgacggccac gacgcctcga tcaacatcat gcgccgcatc
     1561 ctgcaggcct cgggggcgga ggtgatccac ctcgggcaca accgctcggt cgacgaggtc
     1621 gtgggcgcgg cgctgcagga agacgtacag ggcgtcgcgg tgtcgtctta tcagggcggt
     1681 cacctcgagt acttcaagta catgctggac ctgctgcgct cgcaaggcgg cgagcacatc
     1741 aaggtattcg gcggaggcgg cggggtgatc gtgcctgcgg agatcaagga gctgcaggac
     1801 tacgggatcg cgcggatcta ttcgcccgaa gacgggcagc gcatgggcct gcagggaatg
     1861 atcaacgaca tgctcgcgcg ctgcgacttc gatgcctcgc acaaggaccc gaagacactc
     1921 ccgcagatca ttacggcgct ggaggtgggg gttctcaata acaaagagct ccttgaaaaa
     1981 gcgaaaagca atccagctcc cgtgcttggt atcaccggca ccggcggcgc gggcaagagc
     2041 tcgcttaccg acgagctcgt gcggcgcttt cgcctggacc agggcgatgc gctgaagatc
     2101 gcggtgctat cggtggatcc gacgcgccgc aagaccggcg gcgcgctcct cggcgaccgc
     2161 atccgcatga atgcgatcca cgggccgaac gttttcatgc gctcgctcgc gacgcgcgac
     2221 accgggacgg agctttcggc ggcgctcggc gatgcgattg ccgcctgccg cgccgagggc
     2281 tacgggctgg tcatggtcga gaccgcgggc atcgggcagg gcgacgccgc gatcgtgccg
     2341 cacgtcaatg cttcgcttta cgtcatgacg cccgagttcg gtgcggcgag ccagctcgag
     2401 aagatcgaca tgctcgacta cgccgacttc gtcgcgatca acaagttcga ccgccgcggc
     2461 gccgacgacg cgctgcgcga cgtgcgtaag caggtgcagc gcaaccgtga ggccttcagg
     2521 accgcgccag aggacatgcc ggtgttcggc accatagctt cgcgcttcaa cgacgacggc
     2581 gtgacttcgc tctaccacgc ggtggccgga aaactgggat ttcagacaac gaggctgccg
     2641 agacccgccg gcaaggtttc ctctgcggtc catgtcatcg tcccgcccaa gcgccagcgc
     2701 tatctggccg agatcgccga gacggtgcgc ggctatcacc accgcgtcga gcagcaatcg
     2761 cgcctggcgc gcgagcgcca gcaactcttt ggcacccggg cgatgctggg caggagcttt
     2821 gcggagctcg acgcgctgat cgaatcgagg aggctcgacg ccgaagcgca aaggctgctc
     2881 gacggctggc ccaaggtcat caggtcctac tccggcgagg agcagatcgt aaaggtgcgc
     2941 aacaaagaac tgcgcacgca actgacccat acatctcttt caggcacgaa ggtgccgaag
     3001 gtcgcgctcc cgcgctacga ggaccacggc gagcttctgc gctggcgtat gcgcgagaac
     3061 ctgcccggcg agttcccgtt caccgcgggc gtgttccact tcaagcgcga gaacgaggat
     3121 ccgactcgca tgttcgcggg cgagggcgac ccgttccgca ccaacaagcg cttccacctg
     3181 ctctcgcgcg acatgccggc gaagcgtctg tccacagcgt tcgactcggt cacgctgtac
     3241 ggcttcgacc ccgacgagcg gcccgacatc tacggcaagg tgggcaatag cggcgtctcg
     3301 atcgcgacgc tcgacgacat gaaggcgctt tacgcgggct tcgacttgtg cgatccgtcc
     3361 acctcggtat cgatgacgat caacggcccg gcgccgacga tcctcgccat gttcttcaac
     3421 accgcgatcg accagcaggt cgagaagttc aggcaagaga aaggaaaaga tccgtctgcg
     3481 gaagagctgg aggcgataaa ggcccaaacc ctcgcttccg tgcgcggaac cgtccaggcc
     3541 gacatcctga aggaggatca ggggcagaac acctgcattt tctccaccga gttctcgctc
     3601 aaggtgatgg gcgacatcca ggagtacttc atcgccaacc aggtgaggaa tttctactcg
     3661 gtgtcggtgt cgggttacca catcgcggag gcgggggcga acccgatctc gcagctcgcg
     3721 ttcacgcttg cgaacggctt caccttcgtc gaggcctacc tcgcgcgcgg gatgaagatc
     3781 gacgccttcg ctccgaacct gtcgttcttc ttctcctacg gcatggatcc ggagtatgcg
     3841 gtgctcgggc gcgtggcgcg gcgcatctgg gcggtggcga tgaaggagcg ctaccgcggc
     3901 aacgagcgca gccagaagct caagttccac tcgcagacct ccggccgctc gctgcatgcg
     3961 caggaaatcg ccttcaacga catccgcacc acgctacagg cgctgatctc gacctacgac
     4021 aacaccaact ccctgcacac ca
//
LOCUS       DASTPD010000081         6922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_856851, whole genome shotgun sequence.
ACCESSION   DASTPD010000081 DASTPD010000000
VERSION     DASTPD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856851"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFR57_02915"
     CDS             complement(<1..257)
                     /locus_tag="VFR57_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEU0258356.1"
                     /translation="MAARILDGKSLAARLRTEMKPRVDALVQRGVRPGLAVILAGDDA
                     AARVYVRNKTLAAAEIGVHSELHEFKQDVAEAVVLEKVSSL"
     gene            339..3026
                     /gene="aceE"
                     /locus_tag="VFR57_02920"
     CDS             339..3026
                     /gene="aceE"
                     /locus_tag="VFR57_02920"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0258357.1"
                     /translation="MSAVPQFPAANDPDALETQEWLDALEAVLEREGPERAHYLLERL
                     IDKARRSGAYIPFSPNTAYINTIPPHMEERSPGNVALEERIRSICRWNAMVMVARANK
                     NDDELGGHIASFASVGTLFGTGQQHFWNAPHDGHGGDLIYFQGHCSPGVYARSLLEGR
                     LSQDRLLNFRREVDGKGVSSYPHPWLMPDYWQFPTVSMGLGPIQAIYMARFLKYLQAR
                     GLADTASRKVWVFCGDGEMDEPESMGAIGLAAREKLDNLVFVVNCNLQRLDGPVRGNG
                     KIIQELEGDFRGAGWNVIKVIWGSYWDPLLARDKDGILLRVMEETVDGEYQNYKANDG
                     AFVRKNFFGKHPKLLEMVSRMTDEDIWRLNRGGHDPHKVYAAYAAAAKHKGEPTVILA
                     KTIKGFGLGKQGQAKNPTHQLKKVDIATIKEMRDQFAIPIPDEKLEELPFYIPSADSP
                     EMKYLHERRKALGGYLPQRRTKADQVPAVPALSAFEQLLKGSGEGREISTTMAFVRAL
                     TALIRDKEIGRHVVPIVPDEARTFGMEGMFRQLGIFAPEGQKYEPVDKDQVMFYREDK
                     QGQILEEGINEAGAFSSWIAAATSYSHSNRVTVPFYIFYSMFGLQRVGDLAWAAADQR
                     ARGFLLGATAGRTTLNGEGLQHEDGHSHVLSSVIPNCISYDPTYGYEVAVIIQDGLRR
                     MVTNQEDVYYYITLMNENYEHPAMPQGAEQGILKGMYVLRESKSKHKQRVQLLGSGTI
                     LREVEAAAELLEKDWGVAADVWSATSFTELRRDGLAADRWNLLHPEAKARVPYVTKML
                     EERSGPVVAASDYMKTFADQIRSFIPKDRVYRVLGTDGFGRSDSRAKLRHFFEVNRYF
                     VAIAALKSLADQGEGKPKAVADAIRKYGIDPEKADPTIV"
     gene            3023..4633
                     /gene="aceF"
                     /locus_tag="VFR57_02925"
     CDS             3023..4633
                     /gene="aceF"
                     /locus_tag="VFR57_02925"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEU0258358.1"
                     /translation="MSQVVVPDIGDFRDVEVIEVLVKPGDAVSKEQSLITLESDKATM
                     EIPSPSAGVVKQLTIKVGDKVSKGSPILVLEDRGNGEAPPEPEEKPKAQPAQKAAGGE
                     AMTVPVPDIGDFKDIEVIEVLVKPGDTVGKEQSLITLESDKATMEIPSPAAGVVKELK
                     VKVGDKVSKGSAILVLDSKAAPAREPEKAKPAAPAHAPAPAPAAPPMQIHGTRPVPRE
                     PREETRAKPHASPSVRKFARELGVDLPLVQGSGPKGRILHSDVQAFVKGVIQQKAPVA
                     AKAEGGALPFNLPAWPEVDFARFGPIETKALSRIQKLSGPYLHRNWISIPHVTQFDEA
                     DITDLEAFRKAQTVETEKKGFKLTMLAFMIKACVTALKQFPQFNASLDKSGESLTVKK
                     YYHIGVAVDTPGGLVVPVVRDADRKGVFDIAKELGDISKLARDGKLKPGDMQGGTFSI
                     SSLGGIGGTAFTPIINAPEVAILGVSRSVLKPVHSGKNEKGEDIFKARLMLPLSLSYD
                     HRVIDGVSAARFSAFLVSVLSDIRKLIL"
     gene            4634..6343
                     /gene="lpdA"
                     /locus_tag="VFR57_02930"
     CDS             4634..6343
                     /gene="lpdA"
                     /locus_tag="VFR57_02930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0258359.1"
                     /translation="MAGTEVKVPDIGDFKDVEVIEVLVKPGDAIAKEQSLITLESDKA
                     TMEIPSPDAGVVKELRIKVGDKVSKGSLILLLEAAAKAEAPARAPAKTPEAPRTGAPT
                     PAAGADVECDVLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGGVCLNVGCIPSKAL
                     LHTAAIMDGARALASHGIAFGEPKVDLGKLRAFKDKVVGKLTGGLAGMAKMRKVTVIQ
                     GVGTFLDRNRLQVDGKTTIRFRSAIIAAGSQAAKLPFLPEDPRIVDSTGALELRSLPK
                     KMLVIGGGIIGLEMGTVYSTLGARLDVVEMMDGLMLGADRDLVAVWQKFNAPRFDKVM
                     LKTKTTKGEAVKEGIRVSFDGTEAKIYDLVLVSVGRVPNGKKIAADKAGVTVDEKGFI
                     AVDSQMRTNVANIYAIGDIAGNPMLAHKAVHEGHVAAEAASGMKSHFDARVVPSVAYT
                     DPEIAWVGLTEDEAKKNGVPVGVARFPWAASGRAIANARDEGFTKLVFDAETHRIVGG
                     GIVGTGAGDLISELALAVEMGADATDIGKTIHPHPTTAESVGMAAEVFEGVCTDLPPQ
                     RKR"
     gene            6382..6807
                     /gene="rpiA"
                     /locus_tag="VFR57_02935"
     CDS             6382..6807
                     /gene="rpiA"
                     /locus_tag="VFR57_02935"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU0258360.1"
                     /translation="MNQEALKELVAREALKHVVEDAIVGVGSGSTVFKFIDALASMKN
                     RIDGAVAASEASAERLKKHGIRVVDLNSVNELPVYIDGADEVTEHLHMIKGGGGALTR
                     EKITAAVARKFVCIGDSSKLVPVLGKFPVPIEVIPMARS"
BASE COUNT         1336 a         2202 c         2317 g         1067 t
ORIGIN      
        1 ttcagggaac tcactttttc gagaacaacc gcttctgcaa cgtcctgctt gaactcgtgc
       61 agctcggaat gcacgccgat ctcggcggcg gctagggtct tgttgcgcac gtagacgcgc
      121 gcggcggcgt cgtcgccggc gaggatcacg gcgaggcccg gccttactcc gcgctgcaca
      181 agcgcgtcga cccgcggctt catctcggtg cgaagccgcg ccgcgagcga ttttccgtcg
      241 aggatccggg ctgccatgcg ggcggcgcag tctagcattg cccccatttt ctggttaagc
      301 ttgcctcgcc ctcctgcagc aagactgaat tcacacctat gtccgccgtt ccacaattcc
      361 cggccgcaaa cgatcccgac gcgcttgaaa cccaggaatg gctcgatgcc ctggaagcgg
      421 tgctcgagcg cgaggggccg gagcgcgcgc actacctgct cgagcggctg atcgacaagg
      481 cgcggcgttc gggcgcatac atcccgttct cgccgaatac ggcgtatatc aacaccattc
      541 ctccgcacat ggaggagcgc tcgccgggca acgtggcgct cgaagagcgc atccgctcca
      601 tctgccgctg gaatgcgatg gtgatggtcg cgcgcgccaa caagaatgac gatgagctcg
      661 gtgggcacat cgcgagcttc gcgtccgtcg gcacgctgtt cggcaccggg cagcagcact
      721 tctggaacgc gccgcacgac gggcacggcg gcgatctcat ctacttccaa ggccactgct
      781 cgccgggcgt gtacgcgcgc tcgctcctcg agggtaggct ttcacaagac agattgctga
      841 atttccgccg cgaggtggac ggcaagggcg tctcctcgta tccgcatccg tggctgatgc
      901 cggactactg gcagttcccg accgtgtcca tgggcctcgg gccgatccag gcgatctaca
      961 tggcgcgttt cctgaaatac ctgcaggcgc gcggcctcgc cgatacggcg agccgcaagg
     1021 tgtgggtgtt ctgcggcgat ggagagatgg acgagcccga gtcgatgggc gcgatcgggc
     1081 tcgccgcgcg cgaaaagctc gacaacctgg tcttcgtagt caattgcaat ctgcagcgcc
     1141 tcgacggccc ggtgcgcggc aacggcaaga tcatccagga gctcgagggc gactttcgcg
     1201 gcgccggctg gaacgtgatc aaggtgatct ggggctcgta ctgggatccg ctgctcgcgc
     1261 gcgacaagga cggaatcctc cttcgcgtaa tggaggagac cgtcgacggc gaataccaga
     1321 actacaaggc gaacgacggc gccttcgtgc gcaagaattt cttcggcaag caccccaagc
     1381 tgctcgagat ggtctcgcgc atgaccgacg aggacatctg gcgcctgaac cgcggcggcc
     1441 atgacccgca caaggtctac gcggcgtacg cggcggcggc gaagcacaag ggtgagccca
     1501 ctgtgatcct cgccaagacg atcaagggct tcggcctggg caagcagggg caggcgaaga
     1561 accccacgca ccagctgaag aaggtcgaca tcgcgacgat caaggagatg cgcgaccagt
     1621 tcgccatccc gatcccggac gagaagctgg aggagctgcc gttctacatt ccgtcggcgg
     1681 attcgccgga gatgaaatac ctgcacgagc gccgtaaggc gctcggcggc tacctgcccc
     1741 agcgtcgcac caaggcggac caggtgccgg cggtgcccgc gctgtcggcg ttcgagcagc
     1801 ttctgaaggg ctcgggcgag gggcgcgaaa tctcgaccac catggcgttc gtgcgcgcgc
     1861 tgaccgcgct catccgcgac aaggagatcg gaagacacgt ggtgccgatc gtgcccgacg
     1921 aggcgcgcac cttcggcatg gagggcatgt tccggcagct cggcatcttc gcgcccgagg
     1981 gccagaagta cgagccggtc gacaaggacc aggtgatgtt ctaccgcgag gacaagcagg
     2041 gccagatcct cgaggaaggc atcaacgagg cgggcgcgtt ctcgtcgtgg atcgccgccg
     2101 ccacctcgta cagccattcc aaccgggtga cggtgccgtt ttacatcttc tactcgatgt
     2161 tcggcctgca gcgggtcggc gatctcgcgt gggccgccgc cgaccagcgc gcgcgggggt
     2221 tcctgctcgg cgcgaccgcc gggcgcacca cgctgaatgg cgaggggctg cagcacgagg
     2281 acggccactc gcacgtgctc tcgtctgtga ttccgaactg catctcctac gacccgacat
     2341 acggctacga ggtcgcggtg atcatccagg acggattgcg ccgcatggtg acgaaccagg
     2401 aggatgtgta ttactacatc accctgatga acgagaacta cgagcacccc gcgatgccgc
     2461 agggagcgga gcagggaatc ctgaaaggca tgtacgtgct gcgggaatcg aagagcaagc
     2521 acaagcagcg cgtgcagctt ctcggcagcg gaaccatcct gcgcgaagtc gaagcggcgg
     2581 cggagctgct cgagaaggac tggggtgtcg cggccgatgt ctggagcgcg acgagcttca
     2641 ccgagctgcg gcgcgacggc ctagccgccg accgctggaa cctgctgcac ccggaagcca
     2701 aggcgcgggt tccgtacgtg acgaagatgc tggaggagag aagcggtccg gtggtcgccg
     2761 cgagcgacta catgaagacc ttcgccgacc agatccggtc gttcattccg aaagatcggg
     2821 tctaccgtgt gctcggcacc gacggcttcg gtcgctccga ttcgcgcgcg aagctgcgcc
     2881 acttcttcga ggtgaaccgg tacttcgtcg ccatcgcggc gctgaaatcg ctggcggacc
     2941 agggcgaggg caagccgaag gcggtggccg acgccatcag gaagtacggc atcgaccctg
     3001 agaaagccga tccaaccatt gtatgagcca ggtcgtcgtc cccgacatcg gcgacttcag
     3061 ggacgttgaa gttatcgagg tgttggtcaa gccgggcgac gcggtatcca aggaacaatc
     3121 gctcatcacg ctggaatcgg acaaggcgac gatggaaatc ccgtcgccga gcgcgggcgt
     3181 ggtgaagcag ctcacgatca aggtcggaga caaagtctcc aagggctcgc cgatacttgt
     3241 cctggaagac aggggaaacg gcgaagcgcc gcccgagccc gaggaaaaac cgaaggcgca
     3301 acccgctcag aaagccgcgg gcggcgaggc catgacggtg ccggtcccgg acatcggcga
     3361 cttcaaggat atcgaagtga tcgaggttct ggtaaagccc ggcgacacag tcggcaagga
     3421 gcagtcgctc atcacgctcg aatcggacaa ggcgacgatg gagattccct cgcccgcggc
     3481 cggcgtggtg aaggaattga aagtcaaggt cggcgacaag gtgtcgaaag gctccgcgat
     3541 attggtactc gactcgaagg cagcacctgc aagagagccg gaaaaggcca agcccgcggc
     3601 gccagcccac gcgccggcgc cagcgcccgc agcgccgccg atgcagatcc acggcacgcg
     3661 gccggtgccg cgggagccgc gcgaggaaac gcgcgcgaag ccgcacgcca gcccctcggt
     3721 gcgcaagttc gcgcgcgagc tcggcgtcga cctcccgctg gtgcagggaa gcggtccgaa
     3781 ggggcgcatc ctgcatagcg atgtgcaggc attcgtcaaa ggcgtaatcc agcagaaggc
     3841 tcccgtagcc gcgaaggccg aaggcggtgc gctgcccttc aacctgcccg cgtggccgga
     3901 agtcgacttc gccaggttcg gcccgatcga gacaaaggcc ctctcgcgca tccagaagct
     3961 ttcgggaccc tacctgcacc gcaactggat ctcgatcccg cacgtcaccc agttcgacga
     4021 ggccgacatc accgacctcg aagccttccg caaggcgcag accgtcgaga ccgagaaaaa
     4081 gggcttcaag ctcaccatgc tcgccttcat gatcaaggcc tgcgtcaccg cgctgaagca
     4141 gttcccccag ttcaacgcgt cgctcgacaa gtcaggcgag agcctgaccg tcaagaagta
     4201 ctaccacatc ggcgtcgcgg tcgacacgcc gggcggcctc gtcgtcccgg tggtgcgcga
     4261 cgccgaccgc aagggcgtgt tcgacatcgc caaggagctc ggcgacatct ccaagctcgc
     4321 gcgcgacggc aagttgaagc ctggcgacat gcagggcggc accttctcca tctcgagcct
     4381 cggcggcatc ggcggcaccg cgttcacgcc gataatcaac gcgcccgaag tcgcgatcct
     4441 cggtgtctcg cgttcggtct tgaagccagt gcattccggg aagaacgaga agggcgaaga
     4501 catcttcaaa gcccgcctga tgctcccgct gtcgctctcc tacgatcacc gggtcatcga
     4561 cggcgtcagc gccgcccgct tctccgcttt cctggtctcg gtgctttccg acatccggaa
     4621 gctgatcctc tagatggccg gcaccgaggt gaaagtcccc gacatcgggg acttcaagga
     4681 cgtcgaggtc atcgaggtgc tcgtcaagcc gggcgacgcg atcgccaagg agcaatcgct
     4741 catcaccctg gaatcggaca aggccacgat ggaaatcccg tcgccggacg ccggcgtggt
     4801 gaaggagctg cgcatcaagg tcggcgacaa ggtttcgaag ggctcgctga tcctcctgct
     4861 ggaagccgcg gccaaggcgg aagctcctgc aagggcgccc gccaagacgc ccgaggcacc
     4921 gagaacaggt gcgccgacgc ctgcggccgg tgccgatgtg gaatgcgacg tgctcgtgct
     4981 cggtgcggga cccggtgggt attccgccgc attccgcgcg gcggacctcg ggatgaagac
     5041 ggtgctggtc gagcgctatc cgacgctcgg cggggtgtgc ctgaacgtcg gctgcatccc
     5101 ctccaaggcg ctcttgcaca ccgcggcgat catggacggc gcgcgcgctc tcgcctcgca
     5161 cggcatcgcg ttcggcgagc cgaaggtgga tctcggcaaa ctccgcgctt tcaaggacaa
     5221 ggtggtcggc aagctgaccg gcggcctcgc cgggatggcc aagatgcgca aggtgacggt
     5281 catccagggc gtcggtactt ttctggaccg caaccgcctg caagtggacg gcaagaccac
     5341 tatccgtttc agatccgcga tcattgccgc gggctcgcag gccgcgaagc tgccgttctt
     5401 gcccgaggat ccgcgcatcg tggactcgac gggcgcgctc gagctgcggt cgttgccgaa
     5461 gaagatgctc gtcatcggcg gcgggatcat cggcctcgag atgggcacgg tctactccac
     5521 gctcggcgcg cggctcgacg tagtcgagat gatggacggc ctcatgctcg gtgccgaccg
     5581 cgacctcgtg gcggtgtggc agaagttcaa cgctccgcgc ttcgacaagg tcatgctcaa
     5641 gaccaagacg accaagggcg aggccgtaaa agagggaatc cgggtgagct tcgatggcac
     5701 cgaggcgaag atctacgacc tcgtcctcgt ctcggtcggg cgcgtgccga atggcaagaa
     5761 gattgctgcg gacaaggccg gggtgacggt ggacgaaaag ggattcatcg cggtcgacag
     5821 ccagatgcgc accaacgtcg cgaacatcta cgccatcggc gacatcgcgg gcaatccgat
     5881 gctggcgcac aaggcggtgc atgagggaca cgtcgcggcg gaggcggcca gcggcatgaa
     5941 gtcgcacttc gacgcgcgcg tcgtaccgtc ggtggcgtac accgatccgg agatcgcctg
     6001 ggtcgggctg accgaagatg aggcgaagaa gaatggcgtg ccggtcgggg tcgcgaggtt
     6061 cccgtgggcg gcgtccggtc gcgcgatcgc caacgcacgc gacgaaggct tcaccaagct
     6121 cgtcttcgac gccgagacgc accgcatcgt cggcggcggc attgtcggca ccggcgccgg
     6181 cgacctgatc agcgagctcg cgctcgcggt cgagatgggc gccgacgcca ccgacatcgg
     6241 caagaccatc cacccgcatc cgaccaccgc cgaatcggtc ggcatggcgg ccgaggtatt
     6301 cgagggcgtc tgcacggact tgccgccgca gcgcaaaagg taaagacgag gacgtaaaga
     6361 ctagggcgta aagaccaggg catgaatcag gaagcgctca aagagctcgt tgcgcgcgag
     6421 gcgctgaagc acgtcgtcga ggacgcgatc gtcggcgtgg gctccggctc cacggtgttc
     6481 aagttcatcg acgcgctcgc ctcgatgaag aaccgcatcg acggcgccgt ggcggcgtcc
     6541 gaggcgagcg cggagcggct gaagaagcac ggcatccgcg tggtcgatct caacagcgtg
     6601 aacgagctgc ccgtgtacat cgacggcgcc gatgaggtga ccgagcacct gcatatgatc
     6661 aagggcggcg gcggcgcgct cacgcgcgag aagatcaccg ccgcggtggc ccggaagttc
     6721 gtgtgcatcg gcgacagctc gaagctcgta ccggtgctcg gcaagtttcc ggtgccgatc
     6781 gaggtgatcc cgatggcgcg cagctaggtc gcgcgcgaga tcgtgaagat gggcgggcat
     6841 ccccggctgc gccagggctt caccaccgac aacggcaacg tcgtgctcga ctgccacggc
     6901 atgacgctgc tcgacccgcc ga
//
LOCUS       DASTPD010000082         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_653256, whole genome shotgun sequence.
ACCESSION   DASTPD010000082 DASTPD010000000
VERSION     DASTPD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653256"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFR57_02940"
     CDS             <1..720
                     /locus_tag="VFR57_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258361.1"
                     /translation="DGLTLLMTPVATMSIYPHSYAKLRYDPFTDFEPLAHLANFQLAL
                     GVSAKLQAATLADYVALVKKDPNAGYYASAAAGSIPHFFGVMFARAADITMTHVPYKG
                     TAQVLQALATGEIAAATTLAADIGAHARAGRARILATTGAKRSPAFPDVPTFREAGFD
                     IEGTGWYGLFAPAATPGAIVARLSTAAIDAMRAATVRSRLESLGVEPTGLGPRELAAI
                     MKRDYDRWGPVIKASGFKAEN"
     gene            888..1625
                     /locus_tag="VFR57_02945"
     CDS             888..1625
                     /locus_tag="VFR57_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEU0258362.1"
                     /translation="MQSLWMGVERLLADPAFPKGVPLARLVDPGMVAAMSETVLAHVL
                     DWHHHHYVYRLQQQARRWHRLNQYLASDRTIGLLGLGELGSDVARKLAALGFNIAGWS
                     RRPKELPGVTCFTDLDAVLERSDVVVCLLPLTAHTQGILNFRTLRKIRLGGAVINVAR
                     GGHLVAEDLIKVLESGHLAHAYLDVFEHEPLPPEHPLWRHPAITITPHTAALTEPRTA
                     IPKVVENVERLRRGQPPLNLVDIDAGY"
     gene            1767..2204
                     /locus_tag="VFR57_02950"
     CDS             1767..2204
                     /locus_tag="VFR57_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0258363.1"
                     /translation="MVKYADPQLDAVFAALADSTRRGILESLSGGEAAVSELAAPHDM
                     SLPGFMKHLRVLEDAGLVERTKEGRVVSCELSAAPMQAAAAWISRYEKFWTDKLDSLA
                     RYLYQQEELQTWKSSPRSPRSPSPAATRPRRRKSGVRGPTRKR"
     gene            2105..2557
                     /locus_tag="VFR57_02955"
     CDS             2105..2557
                     /locus_tag="VFR57_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0258364.1"
                     /translation="MEKLAQKPSLTLTRRYAAAPEKVWRAWTDPEAIKRWFGPGPGDP
                     VSVAEVGLRVGGRFRIVFGGADGNANECAGVYKEIVPNRRLAFTWHWPRTTPERISLV
                     TLIFKQAGHETELVLRHEEFFDETARNGHKQGWTGSLEKLAAFLRSST"
     gene            2599..3003
                     /locus_tag="VFR57_02960"
     CDS             2599..3003
                     /locus_tag="VFR57_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0258365.1"
                     /translation="MTPLLKEDTMLQDSPLYAYIPARDVDRARKFYEDKLGFKAGQQV
                     ADGVVYEFGKGTACFLYPTPNAGTSRASQAFWQVDDIEREVAELKTRGVNFEEYDMPD
                     MKGENSIYTGGGARAAWFKDSEGNILAVIQSI"
     gene            3019..3378
                     /locus_tag="VFR57_02965"
     CDS             3019..3378
                     /locus_tag="VFR57_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEU0258366.1"
                     /translation="MAYVDGFVCPVPKKNLQAYRRMATKAGKVWREYGALDYKECVAD
                     DVKKGKLTSFPQAVKLKPGEVVFFSWILYRNRKHRDSVNKKVMKDKRIAPMMDPKKLP
                     FDGKRFFWGGFKVVVDL"
     gene            3392..3841
                     /locus_tag="VFR57_02970"
     CDS             3392..3841
                     /locus_tag="VFR57_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0258367.1"
                     /translation="MNEHTVRLTRVFDAPRSRVFECWTRAEHLVHWFGPKGFTLHSCE
                     ADPRPGGIFKLCMRAPDGKDYWVRGEYREVVPPERLVIACSADDDKGIERLHELIEVT
                     LSESRSRTTVALTAVARGPSDEAAKMLKGMDKGWAQTVERLDTLLRK"
     gene            3844..4377
                     /locus_tag="VFR57_02975"
     CDS             3844..4377
                     /locus_tag="VFR57_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0258368.1"
                     /translation="MLKWILMGVAAVGAVFAIVIALQPSDFRIERGATMRAPAAAAFA
                     QVNDFHNWRAWSPWEKIDPALKRSYEGPQAGAGATYAWQGNKDVGEGRMTILESRPGE
                     LVRIKLEFFKPFAATNTAEFTFKPAGDGTAVTWSMSGQNNFLARAICVFVNMDEMVGG
                     QFEQGLAQMKAVVERRS"
     gene            4377..4742
                     /locus_tag="VFR57_02980"
     CDS             4377..4742
                     /locus_tag="VFR57_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0258369.1"
                     /translation="MGNPVVHWELMSKDPAKVSDFYAGIFGWKVQHSPELNYRVVETG
                     GQGGINGGIVKPERDGPWPGNMLFYILVDDLAKYRKKIVAAGGKIHIEEQKVPGMGKF
                     SLFTDPEGRMMGLWKAKMK"
     gene            4739..5155
                     /locus_tag="VFR57_02985"
     CDS             4739..5155
                     /locus_tag="VFR57_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258370.1"
                     /translation="MKPAAPTREARALSVRINRPAAEAYEFLALPENFPKWASGLGAS
                     LCRNGDDWVTETPEGPAIVRFSERNAYGVLDHQVRLPRSGAVYVPLRVVGNDAGCDLV
                     LTLFRRPEMTDAQFAADAEWVLRDLQAAKRLLEDTR"
BASE COUNT          965 a         1734 c         1731 g          847 t
ORIGIN      
        1 gacggcctca cgctgctgat gaccccggtc gcgacgatgt cgatctatcc gcattcgtat
       61 gccaagctgc gctacgatcc gttcacagac ttcgagccgc tcgcgcacct cgcaaatttc
      121 caactcgcgc ttggcgtcag cgcgaagctg caggcggcta ccctcgccga ttacgtagcg
      181 ctggtgaaga aggaccccaa tgcgggctac tacgcctcgg ccgcagcggg cagcatcccg
      241 cacttctttg gcgtgatgtt cgcgcgcgcc gcagacatca cgatgaccca cgtgccgtac
      301 aaaggcactg cgcaggtgct gcaggcgctc gcgaccggcg aaatcgccgc cgcaaccacg
      361 ctcgcggcgg acatcggcgc tcacgcccgc gccggcagag cgcgcatcct cgccaccacg
      421 ggcgcgaagc gttcgccggc gtttcccgat gtgccgacgt tccgcgaagc gggcttcgac
      481 atcgagggca ccggctggta cggcctcttc gcgcctgcgg ccacgccggg cgccatcgtg
      541 gcgcgcctct ccacggcggc gatcgacgcg atgcgcgcgg cgaccgtgcg cagccgcctc
      601 gagagcctcg gcgtcgagcc gaccggcctc ggtccgcgcg agcttgccgc tatcatgaag
      661 cgcgactacg accgctgggg cccggtgatc aaggcgtcgg gcttcaaggc agaaaactga
      721 acatcctctt cgcctcgcag accgaagacc cggcggtctg gctgccgcgc ctgcgccatg
      781 cgctacccga ggatcgcttt ttcaccggtc ccgaaccggg caccgatgtc gcgctggtcg
      841 cgacgccgcc accgggcacc ttcgagccgc tcaagcgcct caagctcgtg cagtcgctgt
      901 ggatgggtgt tgagcggctg ctcgccgatc ccgcctttcc taagggggtg ccgctcgcgc
      961 gcctggtgga cccggggatg gtggcggcga tgtccgagac ggtgctcgcg cacgtgctcg
     1021 actggcacca tcatcactac gtctaccgcc tgcagcagca ggcgcggcgc tggcaccgtc
     1081 tcaaccagta cctcgcgtcg gaccgcacca tcgggctgct cggcctcggc gaactcggca
     1141 gcgacgtcgc gcgcaagctc gccgcgctcg gcttcaacat cgccgggtgg agccgccggc
     1201 cgaaagagct ccccggcgtc acctgtttca ccgacctcga cgcggtgctc gagcggagtg
     1261 acgtcgtcgt ctgtttgctc ccgctcaccg cgcatacgca aggcatcctg aacttccgga
     1321 cgctgcgcaa gatcaggctg ggcggcgcgg tgatcaacgt cgcgcgcggc ggccacctcg
     1381 tcgctgaaga tctgatcaag gtgctcgaat cgggccatct cgcccatgcc tacctcgacg
     1441 tgttcgagca cgagccgctg ccgcccgagc acccgctctg gcgccatccc gccatcacga
     1501 tcacgccgca caccgcggcg ctcaccgagc cgcgcaccgc gatcccgaag gtcgtcgaga
     1561 acgtcgagcg cctgcgccgc ggccagccgc cgctcaacct cgtcgacatc gacgccgggt
     1621 actgaaagat cggggtctga ccccggtctg accccgattt tcagtttcag taaatggtgg
     1681 cgcgcacgcg tcttgcaggc aggtcagttt cagctgtaac tgcgcgcgtc ttgacaggca
     1741 gggcgcgtga aatgatacta aaccatatgg ttaagtatgc agacccgcag ctcgacgcgg
     1801 tgttcgccgc gctcgccgac tccacgcgcc gcggcatcct cgagtcgctg agcggcggcg
     1861 aggcggcggt gagcgagctg gcggcgccgc acgacatgtc gctgcccggt ttcatgaaac
     1921 atttgcgcgt cctcgaagac gcggggctcg tcgagcgcac caaagaaggg cgcgtggtga
     1981 gctgcgagct ctccgccgcg ccgatgcaag ctgcggcggc atggatttcg cgctacgaaa
     2041 agttctggac tgacaagctc gattcgctcg cccgctatct ttatcagcag gaggaattgc
     2101 agacatggaa aagctcgccc agaagccctc gctcaccctc acccgccgct acgcggccgc
     2161 gccggagaaa gtctggcgtg cgtggaccga cccggaagcg ataaagcgct ggtttgggcc
     2221 ggggccgggc gatccagtat ccgtcgccga ggtcggcttg cgcgtcggcg ggcgcttccg
     2281 catcgtgttc ggcggcgccg acggcaacgc caacgaatgc gccggcgtct acaaggagat
     2341 cgtgccgaac cgaaggctcg ctttcacctg gcactggccg cgcaccacgc cggagcgcat
     2401 ctcgcttgtg actctcatct tcaagcaagc cgggcacgag accgagctcg tcctgcggca
     2461 cgaggagttc ttcgacgaaa cggcgcgcaa cggacacaag cagggctgga ccgggtcgct
     2521 cgagaagctt gcggccttcc tccgatcaag cacttagctt cgctgtcgaa tttggccggg
     2581 ccggctcgac tattgggtat gacacccttg cttaaggagg acaccatgct gcaggactca
     2641 ccgctttacg cctatatccc tgctcgggac gtcgaccgcg cgcgcaagtt ctatgaagac
     2701 aagctcggct tcaaggccgg gcagcaggtc gccgacggcg tcgtttacga gtttggcaag
     2761 ggcacggctt gcttcctcta cccgacgccg aacgcaggca cctcgcgcgc gagccaggcg
     2821 ttctggcagg tcgatgacat cgagcgcgag gtggccgagc tgaagacacg cggcgtgaat
     2881 ttcgaggaat acgacatgcc ggacatgaaa ggcgagaaca gcatctatac cggcggcggc
     2941 gcgagggccg catggttcaa ggacagcgag ggcaacatcc tcgccgtgat ccagagcatc
     3001 taactatgga ggtacgacat ggcttacgtt gacgggttcg tttgcccggt gccgaagaaa
     3061 aatctgcagg cctaccgccg catggcgacc aaggccggga aagtgtggcg cgagtacggc
     3121 gcgctcgact acaaggagtg cgtcgccgat gacgtgaaga agggaaagct cacgtccttc
     3181 ccgcaggcgg tgaagctcaa gcccggggag gtggtgttct tctcctggat cctctaccgg
     3241 aaccgcaagc accgcgacag cgtgaacaag aaggtgatga aggacaagcg catcgcgccc
     3301 atgatggatc cgaagaagct gccgttcgac ggcaagcgct tcttctgggg cggcttcaag
     3361 gtggtggtcg acctttgagg tcgcgagcgg gatgaacgaa cacacggtca gactcactcg
     3421 cgtgttcgac gcgccgcgca gccgggtctt cgagtgctgg acccgcgccg agcaccttgt
     3481 gcactggttc gggccgaaag gcttcaccct ccactcgtgc gaggccgatc cgcgcccggg
     3541 cggcatcttc aagctgtgca tgcgcgcgcc agacggcaaa gattactggg tacgcggcga
     3601 gtaccgggaa gttgtgccgc cggagcgcct ggtcatcgcg tgctcggccg acgacgacaa
     3661 ggggatcgag cggctgcacg agctgatcga ggtcacgctc agcgagtcgc gcagcagaac
     3721 cacggtcgcg ctcaccgcag tggcgcgcgg gccgagcgac gaagccgcga agatgctgaa
     3781 gggaatggac aaggggtggg cgcagaccgt cgagcgcctc gacacacttc tgaggaaatg
     3841 aagatgctca aatggatact catgggcgtt gcagcagtcg gggcagtctt cgcaatcgtc
     3901 atcgcgctgc agccctcgga cttccgcatc gagcgcggcg cgaccatgcg cgctcccgcg
     3961 gctgcggcgt tcgcccaggt caacgatttc cataactggc gcgcatggtc gccgtgggag
     4021 aagatagacc cggcgctcaa gcgcagctac gagggtcccc aggccggcgc cggcgcgacg
     4081 tatgcctggc agggcaacaa ggacgtcggc gaaggccgca tgacgatcct cgaaagccgt
     4141 ccgggcgagc tcgtgcgcat caagctcgag ttcttcaagc ccttcgccgc cacgaacacc
     4201 gccgaattca ccttcaagcc ggcgggcgac ggcacggcgg tcacctggag catgagcggg
     4261 cagaacaatt tcctcgcgcg cgcgatctgc gttttcgtga acatggacga gatggtcggc
     4321 ggccaattcg agcaggggct cgcgcagatg aaagccgtag tggaaaggag atcgtgatgg
     4381 gcaaccccgt ggtgcattgg gaactgatgt cgaaggaccc cgcgaaggtg tcggacttct
     4441 acgccggcat cttcggctgg aaggtgcagc acagtcccga gctgaactac cgcgtcgtgg
     4501 aaacgggagg acaaggcgga atcaacggcg gcatcgtaaa gcccgaacgc gacgggccgt
     4561 ggccgggcaa catgctgttc tacatcctcg tcgatgacct cgcgaagtac cgcaagaaga
     4621 tcgtcgccgc cggcggcaag attcacatcg aggagcagaa agtgcccggc atgggcaagt
     4681 tctcgctctt caccgacccc gagggccgca tgatgggact gtggaaggcc aagatgaaat
     4741 gaagcctgcc gcgccgaccc gtgaagcccg cgccctcagc gtgcggatca accgccccgc
     4801 cgccgaggcg tatgaattcc tcgcgctgcc cgagaacttc ccgaagtggg cgtccgggct
     4861 gggcgcgtcg ctgtgccgca atggtgacga ctgggtcacc gaaaccccgg aaggcccggc
     4921 gatcgtgcgc ttctcggagc gcaacgccta cggcgtgctc gatcaccagg tcaggctgcc
     4981 gcggagcggc gcggtttacg tgccgctgcg cgtggtcggg aatgacgcgg gctgcgatct
     5041 cgtcctaacc ctctttcgcc ggcccgagat gaccgacgcg cagttcgccg ccgacgccga
     5101 atgggtgctg cgcgacctgc aggccgccaa gcggttgctc gaagacaccc gctgaatttc
     5161 atctgaaacg ccggcgcgca gccggtaagg gcggcggcgt agtatcgagg atgcgttccc
     5221 ggctgcggca agtgatcgag atctcgcatc tcaccaagac ctacgccacg gggctgc
//
LOCUS       DASTPD010000083         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1266321, whole genome shotgun sequence.
ACCESSION   DASTPD010000083 DASTPD010000000
VERSION     DASTPD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266321"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFR57_02990"
     CDS             <1..438
                     /locus_tag="VFR57_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0258371.1"
                     /translation="LRDEFPVLVGDELAKGSYLFEEFLAANPGKLKFKELKQEVLLHG
                     HCHQKAFDVMPAVEKVLGMVPGLKVSTVQTSCCGMAGSFGYEAEHYDISMKMAELSLL
                     PSIRNAAKETLLVADGTSCRHQIADGAGRQAVHVARLLEQALD"
     gene            complement(435..1367)
                     /locus_tag="VFR57_02995"
     CDS             complement(435..1367)
                     /locus_tag="VFR57_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0258372.1"
                     /translation="MESALDPVVGAVLPVFGLVGCGYLAGRFRIVTQASSAALNQFVY
                     AFALPAMLFVAVYRGSLDEILSGAFLLAVVAATLGTAAVGFFISSLSGAPPAESTMRG
                     LNASFANTGYLGIPLVTVAYGEAAALPAALATVATNFVSFALAIVCLELFVNPRPGAL
                     RRALRGVIMSPLIWPIALAIALVALEVKIPLPLERLATLLAAAAGPCALFAIGLFVSQ
                     LSIRAGAVASWQTCVLKLVLHPLLMALLVLVVLNIDPLWAKIAVVGAALPLGATAFVL
                     AQRYQLLEAETSTGAVVSTAASLLTVSVVMALLG"
     gene            complement(1352..2122)
                     /gene="lgt"
                     /locus_tag="VFR57_03000"
     CDS             complement(1352..2122)
                     /gene="lgt"
                     /locus_tag="VFR57_03000"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU0258373.1"
                     /translation="MLTHPNIDPIAFSIGPLAVRWYGLMYLAGFAIGWWLGARRIAKG
                     QAPITRAQLDDLLFLIVLGVILGGRLGYVLFYKPGYYASHPLEIFAIWQGGMSFHGGL
                     LGVMFAMVFAARRYGVDYVRLMDFLAPLTPLGFAAGRLGNFINAELPGRVTDLPWAMV
                     FPGTDGAPRHPSQLYQFALEGVALFVFLWWFSSKPRPRGQVSAMFLLGYGVLRFVAEF
                     AREPDAFIGYLALGLTMGQWLCLAMILAGAALLAWSRR"
     gene            2140..>3195
                     /locus_tag="VFR57_03005"
     CDS             2140..>3195
                     /locus_tag="VFR57_03005"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0258374.1"
                     /translation="MAYNRRSRLITQGVARSPNRAMLRAVGFGDGDFEKPIVGVANGH
                     STMNPCNAGISPLVERAVQALEQAGAKPQTFGFPTVTDGIGMGTEGMKYSLVSREVIA
                     DAIETAVNGQLMDGVLCVGGCDKNMPGAMMAMARVNVPGIFVYAGTIKPGKWKGQTLT
                     IVSPFEAVGSFTAGKMSEEDFVGIEKNACPTVGACGGQYTANTMSSSFEALGMSLLGS
                     SQMASPDPEKADSAAESARVLMEAVRNDLKPRDIITRKSIENAVALVMATGGSTNAIL
                     HYLAIAAAAGVKWTIDDFERVRRKVPVLCDLKPSGRFVAVDFHRAGGVPQVLKILLEN
                     GVLHGECMTIHGKTMREM"
BASE COUNT          661 a         1001 c         1086 g          447 t
ORIGIN      
        1 ctgcgtgatg agttccccgt cctcgtcggc gatgagctgg cgaaaggctc atacctcttc
       61 gaggaatttc tcgctgcgaa ccccggcaag ctgaagttca aggagctgaa gcaggaagtg
      121 ctgctccacg gccactgcca ccagaaggcg ttcgacgtca tgccggcggt agagaaagtg
      181 ctcgggatgg tgccgggact caaggtctcc acggtgcaga cgagctgttg cggcatggcg
      241 gggagcttcg gctacgaagc cgagcactac gatatttcaa tgaagatggc ggaactctcg
      301 cttctgcctt ccatcaggaa cgctgcaaaa gaaaccttgc tggttgcaga cggcacgagc
      361 tgtcgccatc agatcgccga cggcgccggc cggcaggcag tgcacgtcgc gcgcttgctc
      421 gagcaggcgc tggactagcc gagaagcgcc atcacaaccg acaccgtaag aagcgaagcc
      481 gcggtggaaa ccaccgcgcc ggtcgaagtc tcggcttcaa gcaactggta gcgctgggcg
      541 agcacgaacg cggtggcgcc gagcggaagc gcggcgccca cgacggcgat cttggcccac
      601 aaggggtcaa tgttcagcac gacaagaacg agcagcgcca tcagcagcgg gtgcagcacg
      661 agctttagca cgcaggtctg ccagctcgcc accgcgccgg cacggatcga caactgggaa
      721 acgaacagtc cgatggcgaa aagcgcgcac ggcccggccg ccgcggcgag cagcgtagcg
      781 agccgctcga gcggcagcgg aattttcact tccagggcga ctagagcgat cgccaaagcg
      841 atcggccata tcagcgggct catgattacc cctcgcagcg cgcgccgtag cgcgccgggg
      901 cgcgggttga cgaagagctc gaggcacacg atcgccaggg cgaaagagac gaagttggtc
      961 gccacggtgg cgagcgctgc cggcagcgcg gcggcctcgc cgtaggccac ggtgacgagt
     1021 ggaataccca ggtagccggt attggcgaag gacgcgttca ggccgcgcat cgtgctttct
     1081 gcgggcggag cgccggacag acttgaaatg aagaatccga cggccgcggt gcccaacgta
     1141 gcggccacga ccgcgagcag aaacgccccg ctgaggatct cgtcgagcga gccgcggtac
     1201 acggccacga aaagcatggc gggaagcgcg aacgcgtaaa cgaactggtt cagagctgcg
     1261 cttgaagcct gcgtgacgat ccgaaagcgg ccggcgaggt agccgcagcc gaccagacca
     1321 aagaccggca ataccgcccc gacgacgggg tctagcgccg actccatgcg aggagcgctg
     1381 ccccggcgag gatcatcgcc aggcacagcc actgccccat cgtcaggcca agcgccaggt
     1441 agccgatgaa ggcatcgggc tcgcgggcga actcggcgac gaagcgcagc accccgtagc
     1501 cgagcaggaa catcgcggac acctggccac gcggtcgcgg cttggaggag aaccaccaca
     1561 ggaacacgaa aagcgccacg ccctccagcg cgaactggta gagctgcgat ggatgccgcg
     1621 gcgcgccgtc ggtgcccggg aacaccattg cccacggcag atcggtcacc cggccgggca
     1681 gctcggcgtt gatgaagttg ccgagccttc cggcggcgaa ccccaacgga gtcagcggcg
     1741 cgaggaagtc catcagccgc acgtagtcga cgccgtagcg gcgcgcggcg aacaccatgg
     1801 caaacattac gccgagcaac ccgccgtgga aggacatgcc accctgccag atggcaaaga
     1861 tttcgagcgg atgcgaagcg tagtagccgg gcttgtagaa gagcacatag cccaggcgcc
     1921 cgccgaggat gacgccgagc acgattagaa agaggagatc gtcgagctgc gcgcgggtga
     1981 tcggtgcctg ccccttcgcg attcgccttg cgcccagcca ccagccgatg gcgaagccgg
     2041 cgagatacat gagcccgtac cagcgaaccg cgagcggccc gatcgagaag gcgatcgggt
     2101 cgatgttcgg gtgggtaagc acgctaaaat ttactctaaa tggcctataa ccggcgcagt
     2161 cggctcatta cccagggggt ggcgcgctcc cccaatcgcg cgatgctgcg cgcggtcggc
     2221 ttcggcgacg gtgacttcga aaagccgatc gtcggcgtgg cgaacggcca ctcgaccatg
     2281 aacccgtgca acgccggcat ctctcccctc gtcgagcgcg cggtgcaggc gctggagcag
     2341 gcgggcgcga agccgcagac cttcggcttc ccgaccgtta ctgacggcat cggcatgggg
     2401 acggagggca tgaagtactc gctcgtgtcg cgcgaggtga tcgccgacgc gatcgagacc
     2461 gcggtgaacg ggcaactgat ggacggggtg ctttgcgtcg gcggctgcga caagaacatg
     2521 cctggagcca tgatggccat ggcgcgcgtc aacgtgccgg ggatctttgt ctatgccggc
     2581 acgatcaagc cgggcaagtg gaaggggcag accctcacca tcgtgagccc gttcgaggcc
     2641 gtgggcagct tcaccgcggg caagatgagt gaggaggact tcgtcggcat cgagaaaaac
     2701 gcctgcccta cggtcggcgc ttgcggaggg cagtacacgg ccaacaccat gtcatcgtcg
     2761 ttcgaagcgc tcggcatgag cctgctcggc tcctcgcaaa tggcttcgcc cgaccccgag
     2821 aaggcggact ccgccgccga gtcggcgcgc gtgctgatgg aggcggtgag aaacgacctc
     2881 aagccgcgcg acatcattac gcgcaagtcg atcgagaacg cggtcgccct cgtcatggcg
     2941 accggcggct cgaccaacgc gatactgcac tacctagcga tcgcggcggc ggctggggtc
     3001 aaatggacca ttgatgactt tgagcgcgtg cgccgcaaag tgccggtgct ctgcgacttg
     3061 aagccctccg ggcgcttcgt ggcggtggat ttccaccgcg ccggcggcgt accgcaggtg
     3121 ctgaagatcc tgctcgagaa cggcgtcctg cacggcgagt gcatgacgat ccacggcaag
     3181 actatgcgcg aaatg
//
LOCUS       DASTPD010000084         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_858357, whole genome shotgun sequence.
ACCESSION   DASTPD010000084 DASTPD010000000
VERSION     DASTPD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858357"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /gene="fdrA"
                     /locus_tag="VFR57_03010"
     CDS             <1..1372
                     /gene="fdrA"
                     /locus_tag="VFR57_03010"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEU0258375.1"
                     /translation="FNRVERARYLDSVALMRLSRSLSALPGVEAAALMIGTPANKALL
                     REAGLLAAEGEEAGPNDLVIAVRASEERAGQGAVASALQFLEKKDDSSPRTGPKAKTL
                     AGALELLPEANLALISVPGDFAAQEARKALDAGLSALVFSSGVPLEEEHALKRLANEK
                     GLLLMGPDCGTALLAGVPLAFANAVPRGDIGIVSASGTGLQEVSCLVSRLGAGVSHGI
                     GVGGRDLDERVGALGTLAAIDALERDAGTRVIVLLSKPPAPRAAEAVLERLARCAKRS
                     VVCFLGLEKPGLARTLHEAAEMATGKKIECAEKEIRRVLGRVEALYCGGTLCSEAELI
                     FRRRGLHGYRVVDLGDDRYTRARPHPMIDPELRNEHVARALADPEVGVLLVDIVLGYG
                     AHPDPAGVLLKENLSSKAVVASVVGTDRDPQVRSHQVERLNAAGVLVAPSNALAAEWA
                     ASLSGY"
     gene            complement(1366..2145)
                     /locus_tag="VFR57_03015"
     CDS             complement(1366..2145)
                     /locus_tag="VFR57_03015"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEU0258376.1"
                     /translation="MPHYWFTAEIVGRAAREALAAGEGEVCAVFRRSFYLRFDHKYAC
                     IGDASLGRGPLNALVADFKEPAIGATAVVAAENAVLWEPPPFRGEAHPHLDALRACLD
                     GHVPEEGLGCTILGVHNALSVHAQPALEAVDRWLAGHALGHEAAQLIGLGPGLTPSGD
                     DYLGGVLVALRWLGRGLQADSLWRWLEPRLASRTSEISAAHLAAAASGQVHEALHQVL
                     ENLSAWEVPDLNPSLARLDAVGHTSGWDALAGIVAVARTAQ"
     gene            complement(2120..3334)
                     /locus_tag="VFR57_03020"
     CDS             complement(2120..3334)
                     /locus_tag="VFR57_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU0258377.1"
                     /translation="MAEDLNPAPRLLMGPGPINADPRVLRAMSAQLLGQFDPQFRKYM
                     KEVSELYRAVFQTKNPWTLLIDGTARSGIEAALVSLIEPGDKVLVPVFGRFGHLKVEI
                     ARRCGAEVIPIETEWGKVFPDDVLEREIRKHKPKLVAVSHGDTSTTMAQPLAQLGKLC
                     RQHDALLYVDATATLGGMDLPIDDWQLDVVSAGLQKCLGGPSGSAPLTLNDRAEARIL
                     KRRHTEQGLRKAGERQGEGPIIRSNYFDLAMIMDYWSDRELNHHTEATSMLYAARECA
                     RILLQEGLQSAFARHALASQALLSGLSAMGLKLFGDLRHKMPNVTGVHIPEGIDGDKV
                     RSALLTHFNIEIGTSFGPLHDRIWRIGTMGYNARPDAVLVTLGALEAVLAREGLKLPR
                     GAAVDAALLVYR"
     gene            complement(3327..4226)
                     /gene="puuE"
                     /locus_tag="VFR57_03025"
     CDS             complement(3327..4226)
                     /gene="puuE"
                     /locus_tag="VFR57_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEU0258378.1"
                     /translation="MSTSGYPRDLVGYGAKPPRPKWPRGARLALQFVLNYEEGGENSI
                     LHGDNAAEAFLSEIVGAQPLEGVRHMSMESIYEYGSRGGVWRLLELFARYELPLTVFG
                     VAMAMERNPAAVEAFLKAGHEIASHGWRWINYQYVPLELEREHMQRAIEIHRRLTGVR
                     PLGWYTGRTSPNTRRLVVEDGGFIYDADDYSDELPWYDTRYGRPQLVVPYTLDANDMR
                     FATAQGFSSGTQFFDYLRDCFDTLYADGGRMMSVGLHCRLVGRPGRVAALERFLKYVR
                     TKKAWVARRIDIARHWLKHHPHG"
     gene            complement(4201..>4767)
                     /locus_tag="VFR57_03030"
     CDS             complement(4201..>4767)
                     /locus_tag="VFR57_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0258379.1"
                     /translation="AAGHAGTVPMRLRRDALAGAAECVLATERIVKGHAELVGTVGRI
                     EAKPGAINVIPGETLFTIDVRAPQDSLRKHAVAAIRSQVERVAANRQLDCEIETLQEF
                     GSTACAPRLMEQMERAVERQGLPVRRLPSGAGHDGMALATITGICMLFVRCKGGISHS
                     PLEAISEADAAAGARVLLDFIEHFRLPA"
BASE COUNT          768 a         1600 c         1593 g          806 t
ORIGIN      
        1 ctttaaccgc gtcgagcgcg ctcgctatct cgactcggtc gcgctgatgc gcctgtcgcg
       61 aagcctgtct gcgcttcccg gcgtcgaggc agcggcgctc atgatcggca ctccggcgaa
      121 caaggcgctg ttgcgcgaag cagggctgct cgccgccgag ggcgaggagg cggggccgaa
      181 cgaccttgtg atcgcggttc gcgcttccga ggagcgcgcg gggcagggcg ctgtggcatc
      241 cgctctgcaa tttctcgaaa agaaagacga ctcatctccg cgaacaggac caaaggctaa
      301 gacgcttgcc ggcgcgcttg agcttctgcc cgaggcgaac ctcgcgctta tctcggtgcc
      361 gggcgatttc gccgcgcagg aggcgagaaa ggcgctcgac gccgggctga gcgcgctcgt
      421 gttctcttcg ggcgtgccgc tggaggagga gcacgcgctc aagcgcttgg cgaacgaaaa
      481 aggactgctc ctgatggggc cggattgcgg caccgcgctc ctcgcaggag tgccgctcgc
      541 tttcgccaac gcggtacccc gcggcgacat tggcatcgtg tccgcctcgg gcacgggcct
      601 gcaggaggta tcctgcctgg tctcgcggct cggcgcaggc gtctcgcacg gtatcggcgt
      661 cggcggtcgc gacctggacg agcgcgtcgg cgcgctcggc actttggcag cgatcgatgc
      721 cctcgagcgc gatgcaggca cgagggtgat cgttctgctc tccaagccgc ctgcgccacg
      781 cgcggccgaa gcggtgctcg agcgcctcgc gcgctgcgcg aagcgatcgg tcgtgtgctt
      841 cctgggcctg gagaagcccg ggctggcccg cacgctgcac gaagcggccg aaatggcgac
      901 cggaaaaaaa atcgaatgtg cagaaaaaga aatccggcgt gtgctcggcc gggtagaagc
      961 cttgtactgc gggggtacgc tgtgcagcga ggccgagctc atcttccgcc gccgcggcct
     1021 ccatggctat cgcgttgtcg acctgggcga cgatcggtac acgcgcgccc ggccccaccc
     1081 gatgatcgac ccggagctgc gcaacgagca cgtcgcgcgc gcgctcgcag accccgaagt
     1141 cggcgttctg cttgtggaca tcgtgctcgg ctacggcgcc catcccgatc cggccggcgt
     1201 gttgctgaaa gagaatcttt cttccaaggc cgtggtggct tcggtggtcg ggaccgatcg
     1261 cgacccgcag gtgcgttcgc atcaggtgga gcggctgaac gcggcgggtg tgctcgtcgc
     1321 gccctcgaat gcgctcgccg ccgagtgggc tgcctcgctc agcggctatt gagcggtcct
     1381 tgccaccgcc acgatgccgg cgagcgcatc ccagccggac gtatggccca cggcatcgag
     1441 ccgggcaagg ctcggattca ggtcgggaac ctcccaagcg gaaagatttt cgagcacctg
     1501 gtgcagcgct tcatgcacct gccccgacgc tgcggcggcc aagtgcgctg cgctgatctc
     1561 gctggtccgt gaggcgaggc gcggctcgag ccaccgccac agcgagtcgg cctgcaagcc
     1621 gcggcccagc cagcgcagcg cgaccagcac gccgccgagg tagtcgtcac cggaaggcgt
     1681 gagccccggc ccgaggccga tgagctgcgc agcttcgtgg cccagcgcat ggcctgcaag
     1741 ccatcggtcg accgcttcca gcgcgggctg cgcgtgcacg ctaagcgcgt tgtgcacgcc
     1801 gaggatggtg cagccgagac cctcctcggg gacgtggccg tcgaggcacg cgcgcagcgc
     1861 gtcgaggtgc gggtgcgcct cgccgcgaaa cggcggcggc tcccacaaca cggcgttctc
     1921 ggcggccacc accgcggtag cacctatcgc gggttccttg aagtcggcga caagcgcgtt
     1981 cagcggtccg cgtccgagcg aggcatctcc gatgcatgcg tacttgtggt cgaaacgcag
     2041 atagaagctg cgacggaaga cggcgcacac ctcgccttcg ccggcggcga gggcctcgcg
     2101 tgcggcgcgc ccgacgatct cagcggtaaa ccaatagtgc ggcatcgacg gcagcgccgc
     2161 gcggcagctt caggccttcg cgagcgagaa ccgcctccag cgcgccgagc gtgacaagga
     2221 cggcatccgg gcgcgcgttg tagcccatgg taccaatgcg ccagatgcgg tcgtgcagcg
     2281 ggccgaacga cgtcccgatc tcgatattaa agtgcgtgag cagcgcgctg cgcaccttgt
     2341 cgccatcgat gccctcggga atgtggacgc cggtgacgtt tggcatcttg tgccgcagat
     2401 caccgaagag cttgaggccc atcgccgaaa gccctgacag cagcgcctgc gacgccagcg
     2461 catgtcgcgc aaaggcgctc tgcagacctt cttgcagcag gatgcgggcg cattcgcgtg
     2521 ccgcgtacag catgctcgtc gcctcggtgt ggtggttgag ctcccggtca gaccagtaat
     2581 ccatgatcat cgccaggtcg aaatagttcg agcggatgat cgggccctcg ccttgccgct
     2641 cgccggcctt gcgcaggccc tgctccgtat gtcttctctt caggattcgt gcctccgccc
     2701 tgtcgttcaa cgtcaaggga gcgctaccag aggggccgcc aaggcatttc tgcagcccgg
     2761 cgctcaccac gtcgagctgc cagtcgtcga tcggcaagtc catcccgccg agcgtcgcgg
     2821 tcgcgtccac gtaaaggaga gcgtcatgct gtctgcaaag ctttcccagc tgcgcgagcg
     2881 gctgcgccat cgtggtcgaa gtatcgccat gcgacacggc gacgagcttg ggtttgtgct
     2941 ttcttatctc gcgctccaga acgtcatcgg ggaaaacctt gccccattcg gtctcgatcg
     3001 gaatgacttc cgcgccgcag cgccgtgcga tctccacctt gaggtggccg aagcggccga
     3061 acaccggcac gagcaccttg tcgccgggct cgatgagcga gaccagcgcc gcctcgatac
     3121 cggaccgggc ggtgccgtcg atgagcaacg tccacgggtt cttcgtctgg aataccgccc
     3181 ggtagagctc ggacacctct ttcatgtact tccggaattg cgggtcgaac tgaccaagaa
     3241 gctgcgcgct catggcgcgc agcactcgcg ggtcggcgtt gatcggcccc ggtcccatca
     3301 gcaggcgcgg cgcggggttc aggtcttcag ccatggggat ggtgtttcaa ccagtggcgg
     3361 gcgatgtcga tcctccgcgc cacccacgct ttctttgttc ttacgtattt caggaaccgc
     3421 tccagcgcag ccacccttcc gggcctgccg accagccggc agtgcagccc caccgacatc
     3481 atccggccgc cgtcggcata aagggtgtca aaacaatcgc gcaggtagtc gaagaactgc
     3541 gtgccggaac taaaaccttg tgccgtggcg aagcgcatgt cgttcgcatc cagcgtgtac
     3601 ggcacgacga gctgcggcct gccgtagcgg gtgtcgtacc agggcagctc atcgctgtaa
     3661 tcgtcggcgt cgtagatgaa gccgccgtcc tcgaccacca agcgccgcgt attcgggctg
     3721 gtccggccgg tgtaccagcc gagcggccgt acgcccgtca gccgacggtg gatctcgatc
     3781 gcgcgctgca tgtgctcgcg ctcgagctcg agcgggacgt actggtagtt gatccagcgc
     3841 cagccatggc tcgcgatctc gtgaccggcc ttgaggaacg cttcgaccgc ggccgggttg
     3901 cgctccatgg ccatcgccac gccgaacacg gtgagcggca gctcgtagcg cgcgaagagc
     3961 tcgagcagcc gccatacccc accgcgggag ccgtactcgt agatcgactc catggacata
     4021 tggcgtacgc cctcgagcgg ctgggcgccc acgatctcgg agaggaacgc ctccgcagcg
     4081 ttgtcgccgt ggaggatgga attctcgccg ccttcctcgt agttgagcac gaactgcagc
     4141 gcgagccgcg cacctctggg ccatttcggc cggggcggct tcgccccgta gccgacaagg
     4201 tcacgcgggt agccggaagt gctcaatgaa atccaacagc actctggcgc cggcggcggc
     4261 gtcagcttcg ctgatcgctt cgagcgggct gtggctaatc ccgcccttgc agcgcacgaa
     4321 gagcatgcag attccggtga tcgttgcgag cgccatgccg tcgtgcccgg cgccggaggg
     4381 cagtcggcgg acgggcaagc cctggcgctc gaccgcccgc tccatctgct ccatgaggcg
     4441 cggcgcgcac gccgtggagc caaattcctg caacgtttcg atttcgcagt ccaattgtcg
     4501 atttgcggcc actcgctcga cttgggagcg aatcgctgcg accgcatgct ttctgagcga
     4561 atcctgcggc gcgcgcacgt cgatcgtgaa gagcgtttcg ccgggaatga cgttgatcgc
     4621 gccgggcttc gcctcgatgc gcccgaccgt gccgacgagc tcggcatgcc ctttgacgat
     4681 tctctctgtg gcaaggacgc attccgcagc gccggcgagc gcgtcgcgcc gcaggcgcat
     4741 cggcaccgtg cccgcgtgcc ccgccgc
//
LOCUS       DASTPD010000085         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1674385, whole genome shotgun sequence.
ACCESSION   DASTPD010000085 DASTPD010000000
VERSION     DASTPD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674385"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFR57_03035"
     CDS             complement(<1..582)
                     /locus_tag="VFR57_03035"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258380.1"
                     /translation="MIPALALEDIRVTFVAQDGSRYTAIRDTTLRVAPGEFVSVVGPT
                     GCGKSTLLNIAVGLLQPSAGRVRVFGEPLAGLNRKAGYLFQTEALMPWRTALENVTAG
                     LEFRGVDTAEAKRQAQAWLQRVGLSGFGERYPHQLSGGMRKRVALAQTLILDPEIILM
                     DEPFSALDIQTRQLMENELLELWAANRKSVVFIT"
     gene            complement(585..1598)
                     /locus_tag="VFR57_03040"
     CDS             complement(585..1598)
                     /locus_tag="VFR57_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258381.1"
                     /translation="MRWFILFSAFAVASAHGQKIEKPDVHIEVGGKSALYYLPLVLTE
                     RLGHFKDEGLTNFRISDFAGGTRSLEAVVGGSADVVAGAYEHTINMQSRRQNFQAFVL
                     YGASPQISVAISAKLADKYKSPKDLKGLKVGVSAPGSSTNMVVNYLLAQGGLKPTDVA
                     IIGVGQGATVIAAIEQGRVDLISQTDPAVTMLEKDGKVKVIAETRTPEGTQKIFGGPM
                     PAGSFYAPIEFIKRNPNTVQAMTNAMVRTLIWMQEATPQQILATVPEEYLLGNKAMYL
                     FAFNNVRSAYSKDGYFSDAGAKTTLKALASFNENIKPAQINLAQTYTNEFVKKAHAKL
                     GKK"
     gene            complement(1634..2245)
                     /gene="recR"
                     /locus_tag="VFR57_03045"
     CDS             complement(1634..2245)
                     /gene="recR"
                     /locus_tag="VFR57_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0258382.1"
                     /translation="MTKPPVPTAKGLERLIQALRVLPGVGPRSASRMAFHLLQHERAG
                     AEELAHALADALASVQRCGKCNNFSEEEVCALCRSPRRDRGLLCVVETPADLAMVEQT
                     LSYSGMYFVLMGRLSPLDGVGPRDIGLDRLLERAADGEVHEVILATNFTNEGEATAHY
                     IAEMLRARNVKASRIARGVPLGGELEYVDAGTISQALLDRRGI"
     gene            complement(2242..2565)
                     /locus_tag="VFR57_03050"
     CDS             complement(2242..2565)
                     /locus_tag="VFR57_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0258383.1"
                     /translation="MKGNIGQLMKQAQQMQENMRRMQESLATTEVEGQSGAGMVKVQM
                     TCKYEVKRVAIDPSLVGDDREMLEDLVTAAFNDASRKVESTIAEKMSGMTAGLGLPPG
                     FKLPF"
     gene            complement(2573..4078)
                     /gene="dnaX"
                     /locus_tag="VFR57_03055"
     CDS             complement(2573..4078)
                     /gene="dnaX"
                     /locus_tag="VFR57_03055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0258384.1"
                     /translation="MSYQVLARKWRPRDFASLVGQEHVVRALRHALEQRRLHHAYLFT
                     GTRGVGKTTLARILAKCLNCETGITPQPCNKCSACVEIDAGRFVDLLEVDAATNTKVD
                     EMRQLLETAQYAPTRGRFKVYVIDEVHQLSGHAFNAMLKTLEEPPEHMKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPRDAIASHLANVLTKENIAFEPDGLNLLARAAAGSMRDAL
                     SLLDQAIAHGGGKVGAASVADMLGAIDQTHLVRLLEAVAASDAAGAVAIADEMQARSL
                     SFDTALADLASLLLQVAMAQTVPDAVDDAAVRALAAKFDPESVQLYYQIALQGREDLP
                     LAPDEHGGFVMTVLRLLAFRPEAGAKALAAPQAAKPQTAKPATGWPELVQQLPVSGAA
                     RELARNAELKSREGATFELVVPKAKAYLAERSYVDKLKAALEQQLGSPVTLKVLAGEV
                     TGGSVASLEAGEREARRAAAAQSVNKDGFVQDLVNIFDAKVVDSTIRPEGK"
     gene            complement(4095..4193)
                     /gene="ffs"
                     /locus_tag="VFR57_03060"
     ncRNA           complement(4095..4193)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR57_03060"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4287..4592
                     /locus_tag="VFR57_03065"
     CDS             4287..4592
                     /locus_tag="VFR57_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258385.1"
                     /translation="MLRTLLMVAMLALAACSVDYGQTRSSFQLKVAGKSLEEAVDAAG
                     KPTREENRGPDEKVLVYEKKTFDSENQNAKDAAVRVFFKKDASGKFQYSSIEFVPET"
     gene            complement(4757..4921)
                     /locus_tag="VFR57_03070"
     CDS             complement(4757..4921)
                     /locus_tag="VFR57_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258386.1"
                     /translation="MKPDAVAIVIGILALAAAALFVQWFSGQPPDWSVVKAVGEQFRA
                     AAPGPFIEEQ"
     gene            complement(5035..5793)
                     /locus_tag="VFR57_03075"
     CDS             complement(5035..5793)
                     /locus_tag="VFR57_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258387.1"
                     /translation="MPGFHYRLTDAGRAAWEEQDTAVPAEYRRLLWMMDVQGEGGVAD
                     KLAALYPPDMLAEWLGELEEIGLIERVAESETGAAPIAAEGTVGVDSATMAAAKIAGA
                     SLARTGAYLALERLRYRPRVEKSPADTVILIVEDDPDQLALADLRVSMAGYKVRVAGS
                     VNAFLQALVDEGAPDLLLLDVMLPDGDGFDVLVKMRRHQSLGSLPIVLLTAKTDPGDI
                     GRGLVLGADGYITKPYTKKALVDVIERVLKRPAE"
BASE COUNT          999 a         1947 c         1892 g         1102 t
ORIGIN      
        1 cgtgatgaag accaccgact tgcggttggc cgcccagagc tcgaggagct cgttctccat
       61 gagctggcgc gtctggatgt cgagcgccga gaacggctcg tccatcagga tgatctcggg
      121 atcgagaatc agcgtttgcg ccagcgccac gcgctttcgc atgccgccgc tcaattggtg
      181 cggatagcgc tcgccaaacc cgcttagccc cacgcgctgc agccaggcct gcgcctgccg
      241 cttcgcctcg gcggtatcga cgccacgaaa ctccagtccc gcggtcacat tctcgagcgc
      301 cgtgcgccac ggcatgagcg cctcggtctg gaagaggtag cccgccttgc ggttcaatcc
      361 ggcgagcggc tcgccgaaca cccgcacgcg gcccgccgag ggctgcagca gcccgacggc
      421 gatgttgagc agcgtggatt tgccgcagcc ggtcggcccc acgaccgaga cgaattcgcc
      481 cggcgctacg cggagcgtcg tgtcgcggat ggcggtgtag cggctaccgt cctgcgcgac
      541 gaacgtgacg cggatgtcct cgagcgcgag cgccggaatc aaatctattt cttacccagc
      601 ttggcgtgcg ccttcttcac gaattcgttg gtgtaggtct gcgcgagatt gatctgagca
      661 ggcttgatgt tctcgttgaa cgaggcgagc gccttcagcg tggtcttcgc gccggcgtcg
      721 ctgaagtagc cgtccttgga gtacgcgctc cttacattgt tgaaggcgaa gaggtacatc
      781 gccttgttgc caagcaggta ctcttccggc accgtcgcga ggatctgctg cggcgtggct
      841 tcctgcatcc atattagggt gcgcaccatg gcgttggtca tcgcctgcac ggtattcgga
      901 ttgcgcttga tgaactcgat cggcgcgtag aagcttccgg ccggcatcgg gccgccgaag
      961 atcttttgcg taccttcagg cgtgcgcgtc tccgcgatca ctttcacttt gccgtccttc
     1021 tcaagcatcg tgactgcggg gtcggtctgc gagatgaggt cgacgcgccc ctgctcgatg
     1081 gccgcaatca cggtcgcacc ctgccccacg ccgatgatcg ccacgtcggt gggcttgagg
     1141 ccgccctgcg cgagcaggta gttcacaacc atgttggtgc tcgaaccggg cgcgctgacg
     1201 ccgactttga ggcctttcag atccttcggc gacttgtact tgtcggcgag cttcgcggag
     1261 atcgcgacgc tgatctgcgg cgaagcgccg tagagcacga acgcctggaa gttctggcgt
     1321 cgcgattgca tgttgatcgt gtgctcgtag gcgccggcca ctacgtccgc gctgccgccg
     1381 accacggctt cgagcgagcg cgtgccgccc gcgaagtcgc tgatgcggaa gttggtgagg
     1441 ccctcgtcct tgaagtggcc gaggcgctcg gtgagcacga ggggcaggta gtaaagcgcc
     1501 gatttgccgc ccacctcgat atggacgtcc ggcttctcga tcttttgccc atgggccgac
     1561 gcgacggcga acgccgaaaa aaggatgaac cagcgcatga cgctcctccc cgttagattg
     1621 aaaatcttac taactaaatg ccgcgccggt cgagcagcgc ctgcgagatc gtgccggcgt
     1681 ccacgtactc cagctcgccg ccgagcggca cgccgcgcgc gatgcggctc gccttcacat
     1741 tcctcgcgcg cagcatctcg gcgatgtaat gcgccgtggc ttccccttcg ttggtgaagt
     1801 tcgtggcgag gatcacttcg tggacctcgc cgtcggcggc gcgctcgagc agccggtcga
     1861 ggccgatatc gcgcgggccg acgccgtcga gcggggaaag gcggcccatc aggacgaagt
     1921 acatccccga ataggaaagc gtctgctcga ccatggcgag gtcggccggc gtctccacga
     1981 cgcaaaggag gcctcggtct cggcgcggcg agcggcacag cgcgcagacc tcttcctcgc
     2041 tgaagttgtt gcacttgccg cagcgctgca cgctggcgag cgcatcggcg agcgcgtgcg
     2101 cgagctcctc agcgccggcg cgctcgtgct ggaggaggtg aaacgccatg cggctcgccg
     2161 agcgcggtcc gaccccgggc agcacgcgca aagcctggat gagccgctcg agcccctttg
     2221 cggtcgggac cgggggcttc gtcaaaacgg cagcttgaag cccggcggca ggcccaggcc
     2281 ggcggtcatg ccgctcatct tctcggcaat cgtcgactcg accttgcggc tggcgtcgtt
     2341 gaacgcggcc gtaaccaggt cctcgagcat ctcgcgatcg tcgccgacga gcgaaggatc
     2401 gatggcgacg cgcttcacct cgtacttgca ggtcatctgc accttcacca tgccggcgcc
     2461 cgactggcct tcgacttcgg tggtggcgag gctttcctgc atgcggcgca tgttctcctg
     2521 catctgctgc gcctgcttca tgagctggcc gatgttccct ttcattgaat gctcactttc
     2581 cctcaggccg aatggtcgaa tcgacgacct tggcgtcgaa gatgttcacg aggtcctgca
     2641 cgaaaccgtc cttgttgact gactgcgccg ccgccgcgcg gcgcgcctcg cgctcgccgg
     2701 cctcgagcga ggcgacgctg ccgccggtca cctcgccggc aagcaccttt agcgtcacgg
     2761 gcgaaccgag ctgctgctcg agcgcagctt tgagcttgtc cacgtagctg cgttcggcga
     2821 ggtacgcctt cgccttcggc accacgagct cgaacgtcgc gccttcccgg ctcttcaatt
     2881 cggcattgcg tgcgagctcg cgcgccgcgc cgctcaccgg gagctgctgc acgagctccg
     2941 gccatcccgt ggccggcttc gccgtttgtg gcttcgcggc ctgcggcgct gcaagcgctt
     3001 tagcgccggc ctcgggacgg aaagcgagaa ggcgcagcac cgtcatgacg aatccgccgt
     3061 gctcgtccgg cgcgagcgga aggtcctcgc gaccttgcaa agcaatctgg tagtagagct
     3121 gcaccgattc gggatcaaac ttggcggcaa gcgcacgcac cgccgcatcg tccaccgcat
     3181 cgggcacggt ttgcgccatc gccacctgca aaagcaggct cgcgagatcg gcgagcgccg
     3241 tgtcgaaaga aaggctgcgc gcctgcatct catcggcgat cgcaactgcg ccggccgcat
     3301 ccgacgccgc caccgcctcg agcaggcgca cgagatgagt ttggtcgatg gcgcccaaca
     3361 tgtcggcgac gctcgcagcg ccaaccttgc cgccgccgtg ggcgatggcc tgatcgagaa
     3421 gcgatagcgc atcgcgcatg ctgccggcgg ccgcgcgcgc gagaaggttc aacccgtccg
     3481 gctcgaaggc aatgttttcc ttcgtcagca cgttggcgag atgcgaggcg atcgcgtcgc
     3541 gcggcatctg cttgaggttg aattgcaggc agcgcgagag caccgtcacc ggaatcttct
     3601 gcggatcggt cgtcgccagg atgaacttca tgtgctcggg cggctcctcc agcgtcttga
     3661 gcatcgcgtt gaacgcgtgc ccggaaagct ggtgcacctc gtcgatcacg tagaccttga
     3721 agcgcccgcg cgtgggcgcg tactgcgccg tctccagaag ctggcgcatc tcgtccacct
     3781 tggtattagt cgccgcgtcc acttccagga gatccacgaa acgcccggcg tcgatctcca
     3841 cacaggccga gcacttgttg cacggctggg gcgtgatgcc ggtctcgcag ttcaggcact
     3901 tggcaaggat gcgcgcgagc gtggtcttgc cgacgccgcg cgtgccggtg aaaaggtatg
     3961 cgtgatgcag gcgcctctgc tcgagcgcgt gtcgcagggc gcgcaccaca tgctcctgcc
     4021 ccacaaggct ggcgaaatcc cgcggccgcc acttgcgggc aaggacttgg tagctcatcg
     4081 ctcgatttag attgggtggc gagcctgacc cccggcactc gcaggggccg gctgtggctg
     4141 cttccttccg gacctgacca ggttcaccag actgcaatgc ggggaggccc gccggctccg
     4201 attgtaagtc attcgtaagc ctgcgtcagg cttcggtaag tgcatgccgg cagaatggtt
     4261 agttcgtccc tttgtagaat acgaagatgc taagaacact cctcatggtg gcaatgctcg
     4321 cacttgccgc ctgctcggtc gactacgggc agacgcgaag ttccttccaa ctcaaagtcg
     4381 ccggcaaatc actcgaagag gcggtggacg ccgccggcaa gccgacccgc gaagaaaatc
     4441 gcgggcccga cgagaaagtg ctcgtctacg agaagaagac tttcgacagc gagaatcaga
     4501 acgccaagga cgctgcagtg cgcgttttct tcaagaaaga cgcgagcggg aagttccagt
     4561 actccagcat cgagttcgtt ccagaaacct agttcgaaac gtaagccgcg agtcagcttt
     4621 gagtcagcgc gtcgttggtt gcgggcgcta ggctgtcctc atcctcctcc atcgcgttcg
     4681 cgcgatggct tccaacgccc gcgggcgcca gcccggcggg cgtttttctt actgctcccg
     4741 gtcgacgcgt taacgcttat tgttcttcga tgaatgggcc gggtgcagcc gctctgaact
     4801 gctcgccgac cgccttgacc acggaccagt ccggcggctg cccgctgaac cactgcacga
     4861 acagggccgc cgcggcaagg gcgaggattc ctatgacgat cgcgacggcg tctggtttca
     4921 tgtcggtgcc tcctcggcga cgcaactttg ccccgtcgtt ctaagctgct taaacgagcc
     4981 taaaccggcc cattctcgac gcacgctacc ggaacagagc aggtggcgct accgtcactc
     5041 cgctgggcgc ttgagcacgc gttcgatgac gtcgaccagt gccttcttcg tatagggctt
     5101 ggtgatgtag ccgtccgcgc cgaggacgag gccgcgtccg atgtcgcccg ggtcagtctt
     5161 cgcggtgagc agcacgatcg gcagcgagcc gagcgactgg tgccgacgca ttttcacgag
     5221 cacgtcgaag ccatcgccgt cgggcaacat cacatcgaga agcagaaggt ccggcgcgcc
     5281 ttcgtcgacg agcgcttgca gaaaagcgtt caccgatccg gcgacgcgca ccttatagcc
     5341 cgccatggaa acccgcagat cggcgagcgc aagttggtcc ggatcgtcct caacgatgag
     5401 gatcaccgtg tcggcggggc ttttctcgac gcgcggtcgg tagcgcaacc gctctagcgc
     5461 caggtacgcg ccagtacgcg caagcgaagc gcccgcgatc ttggccgccg ccattgtggc
     5521 cgagtccacg ccgaccgttc cctcagctgc gatgggtgca gcgccggtct cgctctccgc
     5581 gacgcgctcg atgagtccta tctcctcgag ctcgccgagc cattccgcga gcatgtcggg
     5641 cggatagagc gcggcgagtt tatcggcaac tccgccctcg ccctgcacgt ccatcatcca
     5701 aagcaggcgt cgatactcgg ccgggaccgc ggtgtcctgc tcttcccagg cagcgcgccc
     5761 ggcgtcggtc aatcggtagt ggaaaccggg cattccccga gaggctgcga tattgcggag
     5821 agcgtggcag ccaccggtcg atccgtcaac cgaagtcaac cgcgtgaatg cgctctagat
     5881 gcccgagccc ctagaggccg ggttaatggt ccttatgacc taagccgcaa gaagcggttt
//
LOCUS       DASTPD010000086         4350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_655489, whole genome shotgun sequence.
ACCESSION   DASTPD010000086 DASTPD010000000
VERSION     DASTPD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655489"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VFR57_03080"
     CDS             <1..1304
                     /locus_tag="VFR57_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0258388.1"
                     /translation="MEKLSDADVILAYLPPAWIGQNIFSCAQALVTGYCICCPEAPET
                     VVNDMREIGPTYYFAPPRVLEALLTQVSIRMDDAAAAKRWFYRKFMGIASRVGGALLD
                     KKPVSIGDRLLYALGDFFVYGPLRNVLGMSRVRVGYTAGEAIGPDLFRFYRSIGINLK
                     QLYGSTETAVFVCIQPDGEVRSDTVGPAVPGVELKFTPERELLIRSEGLFREYYKNPE
                     ATRAAKDAEGWFHTGDAGFFGAGAHVKIIDRVKDVGKLADGTLFAPKYLENKLKFFPY
                     VKEAVCFGHAREKVCAFINIDPEAIGNWAEKRNLPYSGYADLAAKDEVYDLVRECVEK
                     VNADLAGEPEIANSQIHRFLILHKELDADDGELTRTRKVRRGSIGEKYAELVAALYDG
                     RSSVHVSAQVRYEDGRTGSYAADLKIGDARTFAPAAATKAA"
     gene            1310..2122
                     /locus_tag="VFR57_03085"
     CDS             1310..2122
                     /locus_tag="VFR57_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258389.1"
                     /translation="MTAPETHLHAPTRKIGEVILAVEGVSLAFGGVKALQEVSFDVRA
                     HEVRAIIGPNGAGKSSMLNVLNGVYHPQRGKIRFKGKEFADMEPHRAAVMGIARTFQN
                     IALFKGMSVLDNIMTGRNLRMHSGILAQAIRVGAAEREEIEQRVRVEHIIDFLELQPY
                     RKTQAGRLPYGLQKRVDLARALASEPEVLLLDEPMAGMNLEEKQDMCRYILDANDQLG
                     TTIVLIEHDMSVVMDISDRVVVLDYGRKIADGTPDEVRANQAVIDAYLGASH"
     gene            2167..3132
                     /locus_tag="VFR57_03090"
     CDS             2167..3132
                     /locus_tag="VFR57_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258390.1"
                     /translation="MLVVGLFTPLALKLSAFGFWLEVLLGGLMAGVLYSLVALGFVLI
                     FKASGVFNFAQGAMVLFAALAVARLAERMPLWAAILVAVVIMVILAMVIERVVLRPLV
                     NQEAVALLMATLGVTYFLDGFGQTVWGRDIYAIDLGISREPVMILDKWIQGGILINRD
                     DVAAAVVAILLVSALALFFQKTATGRALRAVADDHQAAQSVGIPLNRIWVIVWAVAGV
                     VAIVAGVIWGGKLGVQFSISLVALKALPVVILGGFTSVPGAIIGGLIIGVGEKMAEVF
                     LGPPLVKMFDLAGGGIENWFAYMLALLFLLVRPEGLFGERHIDRV"
     gene            3134..4198
                     /locus_tag="VFR57_03095"
     CDS             3134..4198
                     /locus_tag="VFR57_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258391.1"
                     /translation="MIYRENGQFKSTYRGDLQMLPILQDRVVIGALVAFAFLVVPFMT
                     SDYLFLSLVIPFLILALAAIGLNLLVGYCGQISIGHAAFMAVGAYAAYNLVLRVPWLN
                     FLVVLVLAGLIAAAAGMLFGIPSLRIKGLYLAVATLAAQFFSDWVFARVKWFTNYAHS
                     GSVSTAPVELFGWRVDTPVEKYLFLLGVLIVFTIIAKNLTRGHLGRAWMATRDMDVAA
                     EVIGIRPVYAKLTAFGVSAFFCGIAGALWGFIHLGSWEPLAFNLTLSLNLLFMIIIGG
                     MGSILGAYYGAAFIVVLPILLNQVPQWLGVPISTATASHLEFMIFGALIVLFLILEPH
                     GLARMWSIGKEKLRLWPFPH"
BASE COUNT          700 a         1401 c         1378 g          871 t
ORIGIN      
        1 agatggagaa gctctcggac gccgacgtaa tcctcgccta cctgccgccg gcttggatcg
       61 gccagaacat tttttcctgc gcgcaggctc ttgtgaccgg ctattgcatc tgctgtccgg
      121 aggcgcccga gaccgtagtc aacgacatgc gcgaaatcgg accgacctat tacttcgcgc
      181 cgcctcgcgt gctggaagcc cttctcacgc aggtctcgat ccgcatggac gacgccgccg
      241 ccgcgaaacg ctggttttat cgcaagttca tgggcatcgc cagccgcgtc ggcggcgccc
      301 tgctcgacaa aaaacccgtg agcatcggcg atcggctgct ctacgccctg ggtgatttct
      361 ttgtgtacgg cccgttgcgc aacgtgctcg gcatgagccg cgtgcgcgtc ggctacaccg
      421 ccggcgaggc aatcggaccg gatcttttca ggttctaccg ctcgatcggc atcaatctca
      481 agcagctcta cggctcgacc gagaccgcgg tgttcgtctg catccagccc gacggcgaag
      541 tgcgctccga caccgtgggc ccggcggtgc cgggcgtgga gctcaagttc acccccgagc
      601 gtgagctcct catccgctcg gaggggcttt tccgcgagta ctacaagaat ccggaggcga
      661 cgcgcgcggc gaaggacgcc gagggctggt tccacaccgg cgatgccggt ttcttcggcg
      721 ccggcgccca cgtcaagatc atcgacaggg tgaaggacgt cggcaagctc gccgacggca
      781 cgctgttcgc gccgaagtat ctcgagaaca agttgaaatt cttcccttac gtcaaggaag
      841 cggtctgctt cggccacgct cgcgagaagg tctgcgcgtt catcaatatc gaccccgagg
      901 cgatcggcaa ctgggccgag aagcgcaacc tgccgtactc gggctacgcc gacctcgccg
      961 cgaaggacga ggtctacgac ctcgtgcgcg agtgcgtgga aaaggtgaac gccgacctcg
     1021 cgggcgagcc cgagatcgcc aattcgcaga tccaccgctt cctcatcctg cacaaggagc
     1081 tcgatgccga tgacggcgag ctcacgcgta cgcgcaaggt gcgtcgcggc tccatcggcg
     1141 agaagtacgc tgagctcgtg gcggcgctct atgatggaag aagctcggtg cacgtaagcg
     1201 cgcaagtgcg ctacgaggac ggccgcaccg gcagctacgc cgccgatctc aagatcggcg
     1261 acgccaggac cttcgcgccg gctgcggcga cgaaagcggc gtaggccgga tgaccgcgcc
     1321 ggagacgcac ttgcatgcgc cgacccgaaa gatcggcgag gtcatcctcg ccgtcgaggg
     1381 cgtgtcgctc gcttttggtg gagtgaaggc gctgcaggag gtgtccttcg acgtgcgcgc
     1441 gcacgaggtg cgcgccatca tcgggcccaa cggggcgggc aagtcctcga tgctcaacgt
     1501 gctgaacggc gtctaccacc cccaaagggg caagatccgc ttcaagggaa aagagttcgc
     1561 cgacatggag ccgcaccgcg ccgcggtgat gggaattgca cgcacctttc agaacatcgc
     1621 gctcttcaag ggcatgagcg tgctcgacaa catcatgacc ggacggaacc tccgcatgca
     1681 ttccggcatc cttgcgcagg cgatccgtgt cggcgcggcc gagcgcgagg aaatcgagca
     1741 gcgcgtgcgc gtcgagcaca tcatcgactt cctcgagctg caaccctacc gcaagacgca
     1801 ggccgggcgc cttccctatg gactgcaaaa gcgcgtcgat ctggcccgtg ctctcgcctc
     1861 ggaacccgag gtgctgctgc ttgacgagcc gatggcggga atgaacctgg aggaaaagca
     1921 ggacatgtgc cgatacatcc tcgacgccaa cgaccagctc ggcaccacga tcgtcctgat
     1981 cgagcacgac atgagcgtcg tcatggacat ctccgaccgg gtcgtggtgc tcgactacgg
     2041 gcgcaagatc gccgacggca cgcccgacga ggtgcgcgcc aatcaagccg tgatcgacgc
     2101 ttacctcgga gcgagccatt gacgttcgac cttaagcgcc aattgcccgt gctcgcgctc
     2161 gggctcgtgc tcgtcgtcgg actcttcacg ccgctcgcgc tcaagctttc ggccttcggc
     2221 ttctggctcg aggtgctgct cggcgggctc atggccggcg tgctctattc attggtggcg
     2281 ctcggcttcg tcctcatctt caaggcctcg ggcgtcttca atttcgcgca gggcgccatg
     2341 gtgctgttcg ccgccctcgc ggtcgcccgg cttgcggagc gcatgccgct ctgggccgcg
     2401 attctggtcg cagtcgtaat catggtgatc ctcgccatgg tgatcgagcg cgtcgtactg
     2461 cgcccgctcg tcaaccagga agcggtggcg ctcctcatgg cgacgctcgg ggtcacgtac
     2521 tttctcgacg gcttcggcca gacggtgtgg ggccgcgaca tttatgccat cgatctcggc
     2581 atctcgcgag agccggtaat gattctcgac aagtggatcc agggcggcat cctgatcaat
     2641 cgcgacgacg tcgcggcggc ggtcgtggcc atcctgctcg tgagcgcgct cgcgcttttt
     2701 ttccagaaaa cggcgaccgg ccgcgcgctg cgtgcggtcg ccgacgatca tcaggccgcg
     2761 cagtcggtcg gcattccgct caaccgcatc tgggtcatcg tctgggcggt ggcgggcgtg
     2821 gtggcgatcg tggccggcgt gatttggggc ggcaagctcg gcgtacaatt ctcgatctcg
     2881 ctcgttgcgc tgaaagcgct gcccgtcgtc attttggggg gtttcacgtc ggtgccgggc
     2941 gccatcatcg gcggcctgat catcggcgtg ggcgagaaga tggccgaggt ctttctcggt
     3001 ccgccgctcg tcaagatgtt cgacctggcc gggggtggga tcgagaattg gttcgcttac
     3061 atgctcgcgc tgctctttct actcgtgcgg cccgagggcc tgttcggcga gcgccacatc
     3121 gatcgggtct agggtgatct accgggaaaa cggccagttc aagtcgacct accgcggcga
     3181 cctgcagatg ctgccgatcc tgcaggaccg ggtcgtcatc ggcgcactcg tcgcgtttgc
     3241 gtttctcgtg gtgccgttca tgacgagcga ttaccttttt ctttcgctgg tcatcccctt
     3301 tcttatcctc gcgctcgcgg ccatcggtct caatctgctc gtgggctatt gcggtcagat
     3361 ctccatcggc catgccgcgt tcatggccgt cggcgcctac gcggcttaca acctggtcct
     3421 gcgcgtcccc tggctcaatt tcctcgtggt ccttgtgctt gccggtctca ttgctgcggc
     3481 ggccggaatg ctcttcggaa ttccgagcct tcgcatcaaa ggcctgtacc tggcggtggc
     3541 cacactcgcc gcgcagttct tttccgactg ggttttcgcc cgggtgaagt ggttcaccaa
     3601 ctatgcgcat tccggttcgg tgtccaccgc gcccgtggaa cttttcggct ggcgtgtcga
     3661 cacgccggtc gaaaagtatc tgttcctcct cggcgtgctg atcgtcttca ccatcatcgc
     3721 gaagaatctc acgcgcggac atctcggccg cgcctggatg gcgacgcgcg acatggacgt
     3781 ggccgccgaa gtgatcggca ttcggcccgt gtatgccaag ctcaccgcgt tcggggtcag
     3841 cgcgttcttc tgcggcatag cgggcgccct gtggggattc atccacctcg gctcctggga
     3901 gccgctcgcg ttcaacctca cgctctcgct gaacctgctc ttcatgatca tcatcggggg
     3961 tatggggtcg attctcgggg cgtactacgg ggcggccttc atcgtcgtgc tccccatcct
     4021 gctcaaccag gtgccgcagt ggctcggcgt gccgatctcg accgccacgg cttcgcacct
     4081 ggaattcatg attttcgggg cgctgatcgt gcttttcctg atcctggagc cccacgggct
     4141 cgcgcgcatg tggtcaatcg gcaaggagaa gctccggtta tggccgttcc cgcactgacg
     4201 aagctattat ctgaaggtct ttttttgagg aggatggaaa tgacatcgaa actgacggtg
     4261 ggcctcgcct gcggggccct ggccgccgcg gccgcgctga cgagcgcgcc ggcttatgcc
     4321 caggcgaagg agcagttctt tccggtgctc
//
LOCUS       DASTPD010000087         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1472938, whole genome shotgun sequence.
ACCESSION   DASTPD010000087 DASTPD010000000
VERSION     DASTPD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472938"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFR57_03100"
     CDS             complement(<1..533)
                     /locus_tag="VFR57_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0258392.1"
                     /translation="MFDAKAFVAGLPNLPGVYRMLGKGGEPLYVGKARELKKRVASYF
                     NKTGHGPRTQVMIGQVASIEVTLTRSEGEALLLENNLIKSLAPRYNILFRDDKSYPYL
                     TITGHRFPRLGFHRGAKDRRHRYFGPFPHAYAVRESIQLLQRVFQLRTCEDTVFEHRS
                     RPCLLHQIKRCTAPCTG"
     gene            593..1159
                     /gene="efp"
                     /locus_tag="VFR57_03105"
     CDS             593..1159
                     /gene="efp"
                     /locus_tag="VFR57_03105"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0258393.1"
                     /translation="MAKVEAVEIRPGNLIEWDKRVWRVLKAYHVHVGGRGGAFMQVEM
                     KDIEVGTKTNQRFRTEDKIERAFVDPREMQYLYQDGDSYVFMDKEDYEQLSLPAEFLE
                     GQAGYLLPNTEVQINFYNGRAIGIEMPPSVVLTVVDTEPGIKNATATNTFKPAKMETG
                     ITVQVPAFINVGEKIKIDTAEGTYMERA"
     gene            complement(1187..2209)
                     /gene="nagZ"
                     /locus_tag="VFR57_03110"
     CDS             complement(1187..2209)
                     /gene="nagZ"
                     /locus_tag="VFR57_03110"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU0258394.1"
                     /translation="MRRLKAALGSAVIDVVGTALSDDDRARLRHPATGAVILFSRNYE
                     HPEQLRLLVQDIRRLREPELLVCVDHEGGRVQRFREGFTAIPPMRALGRLWDRDREVA
                     KEAARRAAYVIGAELGAHGVDFSFAPVLDLDYGLSMVIGDRALHFDPNAVGALAAALV
                     RGFADAGMAAVGKHFPGHGFAEADSHVDVPKDERSLAEILRKDVAPYRAALQAGLAGV
                     MPAHVIYTRCDPEPAGYSKYWLQQILRGRLGFEGLIFSDDLSMAGASTAGGPPARARA
                     ALAAGCDMVLLCNDPEGQNLLLESLKDIPPPNSERLERMRKKGGRDLRKSVAYREAQG
                     ALAALA"
     gene            complement(2197..>2614)
                     /gene="acpS"
                     /locus_tag="VFR57_03115"
     CDS             complement(2197..>2614)
                     /gene="acpS"
                     /locus_tag="VFR57_03115"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU0258395.1"
                     /translation="NEGADGPGSGAVIYGIGTDVVEIARVEKALARWGERFAERILCE
                     PELARFRNHKQPVAYLAKRFAAKEAFTKALGTGIRAPANWHGVWVVNRPSGKPELEFS
                     RELGLLLSARRIARSHLTLTDERGVAVATVILECAG"
BASE COUNT          477 a          854 c          852 g          431 t
ORIGIN      
        1 ttgccggtgc acggagccgt gcagcgcttg atctggtgca gcaggcacgg tcgcgagcgg
       61 tgctcgaaca cagtgtcctc gcaggtgcgc agctggaaca cgcgctgcag cagctggatg
      121 ctctcgcgca cggcgtaggc gtgcggaaag ggcccgaaat agcgatggcg cctgtccttc
      181 gcgccgcgat gaaagcccag gcgcggaaac cggtgtccgg taatcgtcag atagggatag
      241 gacttgtcgt cgcgaaagag gatgttgtag cgcggcgcga ggctcttgat gaggttattc
      301 tcgagcaaca gcgcttcgcc ctcggagcgc gtcagcgtca cctcgatcga cgccacctgc
      361 ccgatcatca cctgcgtgcg cggtccgtgg ccagtcttgt tgaagtacga ggcgacgcgc
      421 ttcttgagct cgcgtgcctt gccgacatag agcggctccc cacccttgcc gagcatgcgg
      481 tagacgcccg gaagattggg caggcccgcg acgaatgcct ttgcatcgaa catgctgaat
      541 tcattataaa acgtgtaaaa tgcgcgcctt ttcgcggcgt caactccacg tcatggcaaa
      601 ggtcgaagca gtcgagatcc gtcctggcaa cctgatcgaa tgggacaagc gcgtgtggcg
      661 cgtgctcaag gcctaccacg tgcacgtcgg cggccgcggc ggcgctttca tgcaggtgga
      721 aatgaaggac atcgaggtcg gcaccaagac caaccagcgc ttccgcaccg aagacaagat
      781 cgagcgcgcg ttcgtcgacc cgcgcgaaat gcagtatctg taccaggacg gcgacagcta
      841 cgtgttcatg gacaaggaag actacgagca gctttctctg cccgccgagt tcctcgaggg
      901 ccaggctggc tatcttctcc cgaacaccga agtgcagatc aatttctaca acggccgcgc
      961 catcggcatc gagatgccgc cgtcggtggt cctcaccgta gtcgacaccg agccgggcat
     1021 caagaacgcc accgcaacca acacgttcaa gccggcgaag atggagaccg gcatcacggt
     1081 ccaggtgcct gccttcatca acgtgggcga gaagatcaag atcgacaccg ccgaaggcac
     1141 ctacatggag cgggcgtagg aataatcggg gactgacccc gatgcgttag gcaagagcgg
     1201 ccagcgcgcc ctgcgcttcg cggtacgcga cgctcttgcg caagtcgcgg ccgccttttt
     1261 tgcgcatgcg ctccaggcgc tcggagttcg gcggtggaat atccttcagc gactcgagca
     1321 gcagattctg cccttcgggg tcgttgcaga ggagcaccat gtcgcaacct gccgcgagcg
     1381 cggcgcgcgc gcgcgccggc ggccctccgg cggtgctggc gcccgccatg gagaggtcat
     1441 cgctgaagat gagaccttcg aagccgagcc tgccgcgcag aatttgctgc agccagtact
     1501 tcgagtaccc cgcgggctcc ggatcgcacc gggtgtagat cacgtgcgcc ggcatcacgc
     1561 cggccaggcc ggcctggagc gccgcccggt acggggcgac gtccttcctg aggatctcgg
     1621 ccaggcttct ctcatccttt ggcacgtcga cgtgggagtc ggcctcggcg aagccgtggc
     1681 cgggaaaatg cttgccaacc gccgccatgc cggcgtcggc gaatccccgg accagggcgg
     1741 cggcgagcgc gcccacggcg ttgggatcga aatgcagcgc gcggtcgccg atcaccatgc
     1801 tcaacccgta gtcgaggtcg agcaccggcg caaagctgaa gtcgacgccg tgggcgccga
     1861 gctcggcgcc gattacgtaa gccgcgcggc gcgccgcctc cttcgcaacc tcccggtcgc
     1921 ggtcccaaag ccgcccgagt gcgcgcatcg gcgggatggc ggtgaagccc tcgcggaagc
     1981 gctgcaccct ccccccctcg tggtccacgc agacgagcag ctcgggttcg cgcagtcttc
     2041 gaatatcctg aaccagaagg cgcagttgct cgggatgctc gtagttgcgc gagaagagaa
     2101 ttacggcacc ggttgccgga tggcgcaggc gggcgcggtc gtcgtcgctc agcgccgtgc
     2161 cgacgacgtc gatgaccgcg gagcccagcg cagcctttag cctgcgcact cgagaatgac
     2221 ggtggccacc gcgaccccgc gctcgtcggt gagcgtcaga tgcgagcgcg cgatgcggcg
     2281 cgcggacaag agcagcccga gctcccggga aaactcaagc tccggcttcc ccgacggccg
     2341 gttcacgacc cagaccccgt gccagttggc gggcgccctg attccggttc cgagcgcctt
     2401 cgtgaacgct tccttggccg cgaagcgttt cgcgaggtag gccacgggtt gtttgtgatt
     2461 gcggaaacga gcaagctcgg gctcgcagag tatgcgctcg gcaaagcgct caccccaccg
     2521 cgcaagcgct ttctcgaccc gggcgatctc tacgacgtcg gtgccgatac cgtagatcac
     2581 cgcgccgctg cctggaccat cagcgccttc attt
//
LOCUS       DASTPD010000088         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1269352, whole genome shotgun sequence.
ACCESSION   DASTPD010000088 DASTPD010000000
VERSION     DASTPD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269352"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFR57_03120"
     CDS             complement(<1..300)
                     /locus_tag="VFR57_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258396.1"
                     /translation="MHADLQAQLAFHLTGRSLPPMEPFELIPALLARYRDLTALRYDF
                     PLVLSKEGKVGSLSTLVDHALEGKDEKTRAQVLRREREIRSGTNQTLASFPVRLAA"
     gene            complement(290..1204)
                     /locus_tag="VFR57_03125"
     CDS             complement(290..1204)
                     /locus_tag="VFR57_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0258397.1"
                     /translation="MNDIACVLRLYDEKYELEDYQGGVPRWCSGCGDNAILASVQRLC
                     RDEGLRPEKTVFVSGIGCSSRFPHYMRTYGFHGIHGRALPVAEGVRLARPDLHVFVNT
                     GDGDCCSIGAAHWIHAVRYNMRMSVFLHDNQIYGLTKKQASPTSPRGTKSNTTPRGST
                     LEALNPLTVTLGVQNVSFVAQAVDWMPDVLYDIVRAAFHHKGFSFVRIIQRCPEWLPD
                     QWEPYIKDPKRVLRLDKSGHDASNLHRAREMASQMDPIPVGILYRNPDLPCYEDLRRS
                     PKLRTAEMVRAGLEAELDKFTVWPEHAR"
     gene            complement(1201..3063)
                     /locus_tag="VFR57_03130"
     CDS             complement(1201..3063)
                     /locus_tag="VFR57_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU0258398.1"
                     /translation="MSEPTLAAKTVTELKEHIVEVISDSGEGAQRCGQSLGSIAARMG
                     IGIWTVEIIPAEIRPPARSVAGASGNRIRLGAGRVTNGGDLTNLVVAFNEQALLGRVR
                     AGELAPRCTILLESMWREHRDPAIRESYEKAVASLRSAKYNVIEIPMGRECNAVVPNA
                     EKGKNMFALGMLCSIYSLELALAREQIALTFGKKAQAVIDSNVKLLEAGYRWGEKNLD
                     FKYRIPAVRPKEPQVVVNGNTALALGVLASGMEICAMYPITPATSASHYLSDVFEKVG
                     GVVHQAEDEIAACAFAIGASYAGKCTVTITSGPGYSLKQEAIGLAVMSEIPLVVINVQ
                     RGGPSTGQPTKVEQGDLMSAIFGSHGDAPKVVMAASTIEDCFYSVITARRIAETFNMV
                     VVVLTDASLATSQQPFPRPQFDAAWMAPPADQSAVPEGLKPYDWDAATGLARRFIPGQ
                     PNGMHTVTGLAHDRESHVAYDPEINEESIHFRSLKLAALQRTLEAPPLFGPPQGDLLV
                     IGWGSTKGAIEEAVESLQREGRKVSSMHLRFLQPLAPGIKEAMSGFKKVMTIEGNWSD
                     RPEDQLIDETNRRYSALATLLRARYLVDVDCWSEVRGQPIKPATIREALRRKLP"
BASE COUNT          487 a          994 c         1078 g          594 t
ORIGIN      
        1 tgcagcaagc cgcactggga agctggcaag ggtctgatta gtaccactgc gaatctctcg
       61 ctcgcgcctg agcacctgtg cgcgcgtctt ctcgtctttg ccctcgaggg cgtgatcgac
      121 cagcgtggac agggaaccga cttttccttc ttttgaaagc accagaggaa agtcgtatcg
      181 gagcgccgtc agatctctgt agcgcgcgag gagcgccggg atgagctcaa agggctccat
      241 cggcgggaga ctgcgccccg tgagatggaa ggcgagctgg gcctggaggt cagcgtgcat
      301 gctcgggcca cacggtgaac ttgtcgagct cggcttcgag gcccgcgcgc accatttccg
      361 cggtgcgcag cttcgggctg cggcgcaggt cctcgtagca gggcaagtcc gggttgcggt
      421 aaaggatgcc gaccgggatc gggtccatct gcgaggccat ctctcgcgcg cggtggaggt
      481 tggacgcgtc gtggccgctc ttgtcgaggc gcagcacccg cttcggatcc ttgatgtacg
      541 gttcccactg gtcgggcagc cactcggggc agcgttggat gatccgcaca aacgagaagc
      601 ccttgtggtg gaacgcagcg cgcacgatgt cgtagagaac gtcgggcatc cagtcgaccg
      661 cttgcgcgac gaaagatacg ttctgcacgc cgagcgtcac ggtgagcggg ttcagcgcct
      721 cgagagtgct gccgcgcgga gtggtgttgc tcttcgtgcc gcgcggcgag gtgggcgagg
      781 cctgcttctt ggtgagcccg tagatctgat tgtcgtgcag gaacacgctc atgcgcatgt
      841 tgtagcgcac ggcgtggatc cagtgcgcgg cgccgatcga gcagcagtcg ccgtcgccgg
      901 tgttcacaaa cacatgcagg tcgggccgcg ccaggcgcac gccctcggca accggcagcg
      961 cgcggccgtg gataccgtgg aagccgtaag tgcgcatgta gtgcgggaac cggctcgagc
     1021 agccgatgcc cgacacgaac acggtcttct ccggccgcag gccttcgtcg cggcacaggc
     1081 gctgcaccga ggcgaggatg gcgttgtctc cgcagcccga acaccagcgc ggcacgccgc
     1141 cctggtagtc ctcgagctcg tatttctcgt cgtagagccg tagaacgcac gcgatatcgt
     1201 tcatgggagt ttcctgcgaa gcgcttcgcg gatcgtggcg ggcttgatcg gctggccgcg
     1261 cacttcgctc cagcagtcca cgtccacgag gtagcgcgcg cgcagcagag tcgcgagcgc
     1321 ggaataccgg cggttggtct cgtcgatcaa ctggtcttcc ggccggtcgg accagttgcc
     1381 ctcgatcgtc atcaccttct tgaagccgct catcgcttcc ttgattcccg gcgcgagcgg
     1441 ctgcaggaag cgcagatgca tcgaggacac cttgcggccc tcgcgttgca gcgattccac
     1501 cgcttcctcg atcgcgccct tggtgctgcc ccagccgatc accaggaggt cgccttgcgg
     1561 cgggccgaaa agcggcggcg cctctagcgt gcgctgcagc gcggcgagct tcaggctccg
     1621 gaagtggatc gattcttcgt tgatctccgg gtcgtaggcg acgtggctct cgcggtcgtg
     1681 cgcgaggccg gtgaccgtgt gcatgccgtt cggctgcccc gggatgaagc gccgcgcaag
     1741 cccggtagcg gcgtcccagt cgtagggctt gagcccctcg ggcaccgcgc tttggtcggc
     1801 cggcggcgcc atccacgcgg cgtcgaactg cgggcgcggg aagggctgct gtgaggtggc
     1861 gaggctcgcg tcggtcagga cgaccacgac catgttgaag gtctcggcga tgcgcctagc
     1921 cgtgatcacc gaatagaagc agtcctcgat cgtgctcgcc gccatcacca ccttcggcgc
     1981 gtcgccgtgg ctgccgaaga tcgcgctcat caggtcgcct tgctcgacct tggtcggctg
     2041 gccggtgctc gggccaccgc gctgcacgtt gatcaccacg agcgggattt cgctcatcac
     2101 cgcgagcccg atcgcttcct gcttgagcga atagcccggg ccggaggtga tggtcacggt
     2161 gcacttgccg gcgtaggagg cgccgatcgc gaacgcgcac gccgcgattt cgtcttcggc
     2221 ctggtggacc acgccgccca ctttctcgaa cacgtccgag agatagtgcg acgctgaagt
     2281 cgccggcgta atcgggtaca tcgcgcagat ttccatcccc gaggcgagca cgccgagcgc
     2341 aagagcggtg ttgccgttca ccacgacctg cggttccttc ggccgcacgg ctgggatgcg
     2401 gtacttgaag tcgaggttct tctcgcccca gcggtagccc gcctcgagca gcttgacgtt
     2461 ggagtcgatg acggcttgag ccttctttcc gaaggtgagc gcgatctgct cgcgcgcgag
     2521 ggctagctcg agactgtaga tgctgcacag catccccagc gcgaacatgt tcttgccctt
     2581 ttccgcgttg gggacgacgg cattgcattc gcgtcccatg gggatctcga tgacgttgta
     2641 cttggcggaa cgtaacgagg cgaccgcctt ttcgtagctt tcccttattg cggggtcccg
     2701 gtgctcgcgc cacatgcttt cgagcaggat ggtgcagcgc ggggcgagct cgcccgcgcg
     2761 cacgcggccg agcaacgcct gctcgttgaa ggcgactacg aggttggtga ggtcgccgcc
     2821 attggtgacg cgcccggcac cgagccggat gcggttgccg ctcgcgccgg cgaccgagcg
     2881 cgcgggcggg cggatctcgg ccgggatgat ctcgacggtc cagatgccaa tgcccatgcg
     2941 cgcggcgatc gagccgagcg actggccgca gcgctgcgcg ccctcgcctg aatcgctgat
     3001 cacttcgacg atatgctctt tcagctcggt gacggtcttg gcggcgagtg tgggttcact
     3061 catcaggcgg ctcgcaatcg cgcgaagttg tgctggtcgg cggggacacc gaacagtctt
     3121 tcagttccgg cgttcgattg tttttcggaa ata
//
LOCUS       DASTPD010000089         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1065595, whole genome shotgun sequence.
ACCESSION   DASTPD010000089 DASTPD010000000
VERSION     DASTPD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065595"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..905)
                     /locus_tag="VFR57_03135"
     CDS             complement(123..905)
                     /locus_tag="VFR57_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0258399.1"
                     /translation="MKAPSFAYAKPRTLAEAFDLLERPGAKILAGGQSLIPSLNMRLS
                     APELLVDITGLAALRGITLEQGVVRIGALTTHSDIERSAEIPKHIPLLAEAVRHIAHP
                     AIRNRGTLGGSIALADPAAEYPACLVALDATIVLQSRAGERMVRAEEFFKGLFEVDLH
                     AGELVTAVEIPALLKDERSVFLELTRRHGDYAIVGLAGVRKSSERRLAFFGLATKPVL
                     IKPKALDGAKASLPEPAGDLYHSPATKLHLAKVLLERAWNRL"
     gene            983..1936
                     /locus_tag="VFR57_03140"
     CDS             983..1936
                     /locus_tag="VFR57_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258400.1"
                     /translation="MPNLVLLLVLVFSFPAAAQTYPTQPIRLIAPFPPGGSVDIMARL
                     IAEPLAAQLGGKIVIDNRSGASGNIGMEAAARSKPDGYTVVLNTIPLATNQALFDKLT
                     WDPIKDFAPIGMVATAAHVLVIPPKVKVNNIEELVRLARSNPGKLAYASAGVGTTFHL
                     CGEMFKDSTQTFILHVPYRGGGPALADTLGGQVDMSFPTISAAVPHIKAGTLRALAVT
                     DVRRSPLLPDVPTLREAGVKDFQFTQWLALLAPANTPREVVTRLNEALRATLNSKEVR
                     DKFAQQGFEPFVTSPDEAGKFIVAEVQRFGTLIKTRKITAE"
     gene            1945..2721
                     /locus_tag="VFR57_03145"
     CDS             1945..2721
                     /locus_tag="VFR57_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0258401.1"
                     /translation="MDLGLKDKKVLITGGSKGIGLACAKAFIAEGAKVALVSRSEQNL
                     RKAKESLTTVYTIPADLTDAAAAAAMVEKVDKEFGPIDVLVNSAGAAKRTDADDLTPA
                     AWRAGMDAKYFSYINVIDPLIKRMGKRGKGAVINIIGGGGKVASPTHLAGGAANAALM
                     LATAGLAFAYASKGVRVVGVSPGVTKTERVAEGLKAEAKRANVSEEQAYKQMIARLPM
                     GRPAEPEEIADIVVFAASERGRYLTGANITTDGAASHVVV"
     gene            2814..4019
                     /locus_tag="VFR57_03150"
     CDS             2814..4019
                     /locus_tag="VFR57_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258402.1"
                     /translation="MKRREILKIGAAAAGLGFPAIIRAQPKEVVLLGIWPQTGAFSDV
                     GPILDRGMRMALEERDMKIQGRPVKYIVRDSATQAGAATRRVEEAVDGEGAKFVIGPW
                     SSGVALAVSEVAKRKKVFHYFSGGTEDISGARCHRYSFQWAASPYTAAKTVVDNFMKA
                     NPKARRWHLLVADYAFGWSVEKYIKEVGKTHGIEFVGADRHPLGEREFSNYVQKAAAN
                     RPDAVAMINFGLDAVSGAREIFNFGLTPKVPLIMTWSSGVEELVQLTPEIRENIWVGS
                     NFYYTADTPVAKKFVAAYNAKFGNPPGYAPSAAYGMTRMMLHAFDKAKSVEVRDVIAA
                     LEGMEVQDLVGRMRVDPKTHQTLRPYFFMRCKPKAKMANPLDFADIIATGDTPLPAEY
                     AACKDLGGF"
BASE COUNT          754 a         1371 c         1357 g          657 t
ORIGIN      
        1 cccgtgctca cagccgatgt gcgagccggt aagccccaga tcctccctca gaaaatccac
       61 caggtgctgg cgcgcgggaa tgctgcgcgt cacctgcgtg ccgttcaccg tcatcgtggc
      121 ggtcagagtc tgttccatgc gcgctcgagg aggactttcg ccaggtgaag cttggttgca
      181 ggcgagtggt agagatcgcc cgcgggctcg ggcagcgagg ctttcgcgcc gtcgagggcc
      241 ttgggcttga tgagcaccgg cttcgtcgcg agtccgaaaa acgccagcct gcgctcggag
      301 cttttccgga ctcccgcgag gcctacgatc gcgtaatcgc cgtgccgccg cgtgagctcc
      361 agaaacaccg aacgctcgtc cttgagcagc gccgggatct cgaccgccgt caccagctcg
      421 ccggcgtgaa ggtcgacttc gaacaggccc ttgaagaact cctccgccct caccatgcgc
      481 tcgccggcgc gcgactgcag gacgatcgtg gcgtcgagcg cgacgaggca ggccggatac
      541 tcggcggccg ggtccgccaa cgcgatgctg ccgccgagcg tgccgcggtt gcggatcgcg
      601 gggtgcgcga tgtgccgcac agcctcggcg agaagcggaa tgtgcttcgg gatttcggcg
      661 gagcgctcga tgtcgctatg ggtggtgagc gcgccgatgc gcaccacgcc ctgctcgagc
      721 gtgatgcccc tcaacgcagc gaggcccgtg atgtcgacca gcagctcggg cgccgacagg
      781 cgcatgttga gcgagggaat gaggctctgg ccgccggcga ggatcttcgc gcccgggcgc
      841 tcgagaaggt cgaacgcctc cgccagcgtg cgcggtttcg cataggcaaa cgacggcgcc
      901 ttcaagcccg gccctcctcc tgccgctatc ataaaagaag aaaatcgggg tcagagcccg
      961 atttcgctac aggaggagaa ccatgccgaa cctagtgctg ctgcttgtgc tcgtgttctc
     1021 cttccccgct gcagcgcaaa cctatccgac ccagccaatc cggctgatcg cgccgttccc
     1081 gcccggcggc agcgtggaca tcatggcgcg cctcatcgcc gagccgctgg cggcgcaact
     1141 cggcggcaag atcgtgatcg acaaccgcag cggcgcctcc ggcaacatcg gcatggaagc
     1201 cgcggcgcgg tccaagcccg acggctacac cgtcgtcctc aatacgattc cgctcgccac
     1261 caaccaggcg ctcttcgaca agctcacctg ggacccgatc aaggatttcg cgccgattgg
     1321 catggtggcg accgcggcgc acgtgctggt gattcccccg aaagtcaaag tcaacaacat
     1381 cgaagagctg gtgcggctcg cgcgctcgaa tccggggaag ctggcgtacg cctcggcggg
     1441 cgtcggcacc actttccacc tctgcggcga gatgttcaaa gactcgaccc agaccttcat
     1501 cctgcacgtt ccctaccggg gcggcgggcc ggcgctggcc gacacgctcg gcggccaggt
     1561 cgacatgagc ttcccgacca tctccgccgc ggtgccacac atcaaggcgg ggaccctgcg
     1621 cgcgctcgca gtcaccgacg tgcggcgctc gccgcttttg ccggacgttc cgacgctgcg
     1681 cgaggccggc gtcaaggatt tccagttcac gcagtggctc gcgctcctcg cgccggcgaa
     1741 cacgccgcgc gaagtggtga cgcggctcaa cgaagcgctg cgagccacgc tcaactccaa
     1801 ggaagtgcgc gacaagttcg cgcagcaggg cttcgagccc ttcgtcacca gcccggacga
     1861 agccggcaag ttcatcgttg ccgaagtcca gcgcttcggc acgctcatca aaacccgaaa
     1921 aatcaccgcg gagtagaaat cggcatggat ctcggattga aagacaagaa agtcctcatc
     1981 accggcggca gcaagggcat cggtctcgcc tgcgcgaaag cgttcatcgc cgaaggcgcc
     2041 aaggttgcgc tcgtttcccg ttccgaacaa aacctccgga aagcgaagga gagcctgaca
     2101 accgtgtata ccatcccggc ggatctcacc gacgctgctg ccgcggcagc catggtcgaa
     2161 aaggtcgaca aggagttcgg cccgatcgac gtgctggtca attcggctgg cgcggcgaag
     2221 cgcaccgacg ccgacgacct caccccggct gcctggcgag ccggcatgga cgcgaagtac
     2281 ttctcctaca tcaacgtcat cgacccgctc atcaagcgca tgggcaagcg cggcaaggga
     2341 gcggtgatca acatcatcgg cggcgggggc aaggtggcct cgccgactca cctcgcgggc
     2401 ggcgccgcca acgccgcgtt gatgctcgcc accgcgggac tcgctttcgc ctatgcctca
     2461 aaaggcgtcc gcgtggtcgg cgtgagcccc ggcgtcacca agactgagcg cgtcgccgag
     2521 ggtctgaagg ccgaagcgaa gcgcgccaac gtctcggaag agcaagcgta caaacagatg
     2581 atcgcgcggc tgccgatggg ccggcccgcc gagcccgagg agatcgccga catcgtggtg
     2641 ttcgcggctt ccgagcgcgg ccgctacctc accggcgcga acatcactac cgatggagcg
     2701 gcgtcgcacg tagtggtgta gtcggacccc gggacggctc gcgtccattc ggaacacttc
     2761 gaccgccgac gaacagtttg gagtaggctc ccccttttcg cgagggagga gccatgaagc
     2821 gcagagagat cctgaagatc ggcgccgcgg ccgcgggcct gggctttccg gcgatcatcc
     2881 gggcgcagcc gaaggaggtg gtgctcctgg gcatctggcc gcagacaggg gcattctcgg
     2941 acgtcgggcc gattctcgat cgcgggatgc gcatggcgct cgaggagcgc gacatgaaga
     3001 tccaggggcg cccggtgaag tacatcgtgc gcgactccgc gacgcaggcg ggcgcggcga
     3061 cgcggcgcgt cgaggaggcg gtcgacggcg agggcgcgaa gtttgtcatc ggcccatggt
     3121 cgtcgggcgt ggcgctcgcg gtgtccgagg tggcgaagcg caagaaggtg ttccactact
     3181 tcagcggcgg caccgaggat atctccggcg cgcgctgcca tcgctactcg ttccagtggg
     3241 cggcaagccc gtacaccgcg gccaagactg tggtggacaa cttcatgaag gcgaacccga
     3301 aggcgcggcg ctggcacctg ctggtcgccg actatgcatt cggctggtcg gtcgagaagt
     3361 acatcaagga ggtcggcaag acccacggca tcgagttcgt cggcgccgac cgccacccac
     3421 tcggcgagcg cgagttctcc aactacgtgc agaaggcggc ggcgaacagg cccgatgccg
     3481 tggcgatgat caacttcggc ctcgacgcgg tctccggcgc gcgcgagatc ttcaacttcg
     3541 gcctcactcc caaggtgccg ctgatcatga cctggtcctc gggggtcgag gagctggtgc
     3601 agctcacgcc cgagatccgg gagaacatct gggtcggctc gaacttctac tacaccgcgg
     3661 atacacctgt ggcgaagaag ttcgtcgcag cctacaacgc gaagttcggc aacccgcccg
     3721 gctacgcacc ctcggccgcc tacggcatga cgcgcatgat gctgcacgct ttcgacaagg
     3781 cgaaatcggt cgaggtgcgc gacgtgatcg cggcgctgga aggcatggag gtccaggatc
     3841 tcgtcggccg catgcgggtc gatccgaaga cgcaccagac gctgcggccg tacttcttca
     3901 tgcgctgcaa gccgaaggcg aagatggcga atccgctcga cttcgccgac ataatcgcga
     3961 ccggcgacac cccgctgccg gcggaatacg cagcctgtaa ggatcttggc ggcttctagg
     4021 ttctaggttt tgaacttact cctcggccag atccttaacg ggatcgcgac ggggatgctg
     4081 tacgccctga tggggctcgg cctgtcgctg atcaccggga tcctcaacat cccgaactt
//
LOCUS       DASTPD010000090        12272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_249638, whole genome shotgun sequence.
ACCESSION   DASTPD010000090 DASTPD010000000
VERSION     DASTPD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249638"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFR57_03155"
     CDS             complement(<1..1110)
                     /locus_tag="VFR57_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0258403.1"
                     /translation="MKRSLVLIALAAAFAATPAAAQKINLNVVTAGDQNMVDYVKDFL
                     APQFEKNNANVSVKAVGTGPGDAGSQKIAEKIQAQKNNPAWDVDVAVIHQKMAGDLVR
                     DGLLAPYVRDVATGRLVTSEASKNALGTNVAGYVIPMFQSQTAIAYNPALVKNPPKTY
                     EELAAWAKANPRKFGYNGIKGGMSGVAFVVGWVYANTPDSDKLMKGPYDAQTKANWDP
                     ALGKLKEFNRSVVITPGNAGTLDMLNRGEIAMGPVWVDMFYTWMADGKLPPNMRLQLI
                     GPGMPGQPMYYVIPAKAANMAAAKKFVEMATSPQIQAEGIVKKFNWYPGIDAQHVKAQ
                     LDDKTWNKLFVDVTPQDLASKGKVFPIKQYFDDILE"
     gene            complement(1107..2174)
                     /locus_tag="VFR57_03160"
     CDS             complement(1107..2174)
                     /locus_tag="VFR57_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258404.1"
                     /translation="MASAALDITQLEVSYGATPVLHQVSLSVAAGEFVALLGPSGCGK
                     TTLLRAVSGFVPVNAGKISIAGRDITHAPPDKRGMAMVFQSYALWPHMTTAQNLGYGL
                     KLRRVARPDIARRVAEILTMLKLDGFGERNVTQLSGGQRQRVALGRALAISPEILLLD
                     EPLSNLDARIREEVRHEIKALQAKLGITTIHVTHDREEAMVMADRIAILDAGRIAQLG
                     TPEEVYNRPNSPFVAAFMGAGNVLELEARPASDGVEIQAGAHNDAVRVPGFRSAGPLI
                     AHFRSEAARLGPPQEGAPDRLLLRGRIVQASYPGGFYRYAVAVGQNHFMVDDTRRLTV
                     GELVGIGLPVSSLHFFPRRNT"
     gene            2313..2642
                     /locus_tag="VFR57_03165"
     CDS             2313..2642
                     /locus_tag="VFR57_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HEU0258405.1"
                     /translation="MIKPDQDGVQRARILAGGYFFKPSHIVVKVNTPVEILASRESGM
                     VPHDLVIDAPEAGIAVKQDLATEPRKIAFTPKRVGSYAIYCSKKPPLGGASHRERGME
                     GVLEVVP"
     gene            complement(2639..3352)
                     /locus_tag="VFR57_03170"
     CDS             complement(2639..3352)
                     /locus_tag="VFR57_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0258406.1"
                     /translation="MPNSKKVLAGASGYSFKEWKGRFYPERMKPDGMLAFYAERLPTV
                     EINNTFYQMPKATVLENWSRQTPESFRFAIKASRRITHFSRLEAEKARDSVEYLYRNL
                     ATLGDKRGPVLYQLPPFLKKDLARLTDFLAVLPSGHQAAFEFRNDTWFDDEVYAALKA
                     RGASLCHSEREDNEPPPLVETAPWGYVRLRLETYSDQDLERWARRLDETGWQQIYVYF
                     MHEPTAPAYAQTLMRFSGR"
     gene            3351..6029
                     /gene="ligD"
                     /locus_tag="VFR57_03175"
     CDS             3351..6029
                     /gene="ligD"
                     /locus_tag="VFR57_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU0258407.1"
                     /translation="MAKSKLSEYSAKRSFTATPEPAPAVPEARRGPLLFVIQQHSARR
                     MHYDFRLECDGVLKSWAVPKAPSLDPSEKRLAVQTEDHPFDYASFEGVIPPKQYGAGE
                     VIVWDCGVYSPDEASSWVEDRDEAERQVREGLEKGKLSVFLRGEKVKGSFALVRTADK
                     KNWLLIKHKDRFVGAADLTTLERSVLSGTSVEEMKSAPAHQMPAAHLIPVGVEERMPK
                     SLAPMHAELKEVPFNHPDWMWEPKLDGYRVLAFVNDGVSLRSRRGLELAAQFPSLAKE
                     LAEQGPAMILDGELVAFDNGKPSFNALQNRFQLKNERDIAVAERTFPVVFYCFDLLHF
                     AGMNLRPASYADRRRYLAQCLLPSSGVQLVHAADDGVELYKAALAAGFEGVIGKKRQS
                     KYEPGKRSAAWVKIKSTRSAEFVVGGYTKGKGARAPLGALLVGYWDGPKLHYASHVGS
                     GFDDHNLDAVQARLEPLRRKTCPFAGKPELHSPTVWVEPETVAEVKFQEWTKDGFLRA
                     PVFLRLRDDIDPKTVKRAGGGEEAAVPRSEIEDVVAQLASIKANGTIAAGNHTIKLSH
                     LDRVYWPADPALKQPALTKRDLLRYLAQVSPYMLTHLADRPLTMIRMPDGIHGPRFYQ
                     KHWAQEKPAFVDAITVFSEHKDESHEYLLCNNLPTLLWLAQSGTLEFHVMHSRAKLGD
                     DSPVKSTDYATSLDTLEGSILNYPDYVVFDIDPYIYSGKEKAGDEPELNTVAFEKGKE
                     VAFWLRELLNGMSLEPIVKTSGKTGLHVFVPIRRTIDFDAARAVSELVGRHLVGKHPK
                     DITMEWSVPKRTGKIFMDYNMNVRGKTLNVAYSPRGAPGAAVSMPLAWDELAHAHPLD
                     FRITNAAERLAKTGDRWRDALTAKKSLQGALEGAKT"
     gene            6073..6906
                     /locus_tag="VFR57_03180"
     CDS             6073..6906
                     /locus_tag="VFR57_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0258408.1"
                     /translation="MAARSIASLTVSFGLVSIPVKLFSATEASRAISFNLLHKACGSR
                     LRQQYFCIKEEVPVQREDMVKGYEFAKDQYVMFTPEELKALEEAGTGTADISEFVPLE
                     SVDPVYFDKAYYLAPDKGGAKPYALLTKALRESKRCALGRWAARGKQYIVMIRAVNEG
                     LVMQQLMYAGEVRSIRDIDIPKTEVRDAELKLAQQLIEQQASDKFDPSSYTDEVRTRI
                     EAAVQKKVEGQEITLAEAPREGAQVIDLMEALRASLEKKPAKKAAPAAKPAATRKAAK
                     K"
     gene            6908..7612
                     /locus_tag="VFR57_03185"
     CDS             6908..7612
                     /locus_tag="VFR57_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0258409.1"
                     /translation="MQQFGVREVEKLLRLPRRTIHGLVKAGFVSPARGPGQTYRFSFQ
                     DLIVMRTAQALAAANVPAKRITSSLKELRRHLPASMPLSGLSIGAVGDRVVVKEGASR
                     WQAESGQYLLAFEGDPAAGELKVMQREPDNVEDEIDRGYALHEAGKLAEAEAVYRDAL
                     ETWGPDALLFYNLGVLLEDLGRKSEALQAYQAALGADPYMADCHYNLALLFDELGRGQ
                     DAIRHMAQYRRLVAKK"
     gene            7725..9173
                     /locus_tag="VFR57_03190"
     CDS             7725..9173
                     /locus_tag="VFR57_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258410.1"
                     /translation="MSENQPWMLFPDNPALSIFVLAIVALVFLYAARRPMHELLRALG
                     ALLGGPLRLGARWLVTTSEQMKERNRAVLLAHGKHEVGQRIEREFERLGAVVRRDLQG
                     YPTLQRKLLDEITRIEEDYKKCGEVPPPPPDWVEAVTAMAEVKTGNEIVQRILEEIKT
                     SIGKIHDKAISEYRKSYESRHKILESFMPFWRSVDRNIAEVNKKLGDLQESAKGVDAL
                     MAKYEAINAKTDKAQHALTVSAFTQFAIALLVMAVAAGGAFINYKLIALPMSEMVGAG
                     DYLTASLRTSEVAALVIIFVEASMGLFLMEALRITHLFPRIATLSDHMRRRMMWIALV
                     LLVTLAGVEAALALMRDMLIADKQALIQSLATVQQAAPADGWVGKIPTAGQMLLGFIL
                     PFALAFIAIPVESLIHSSRTVGGVLLAALVSALALTLRVLANVVRRMSKVLITVYDVV
                     IVLPLMVERMTKAGMAMRHSAVDADDTAYKTR"
     gene            9176..9838
                     /locus_tag="VFR57_03195"
     CDS             9176..9838
                     /locus_tag="VFR57_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0258411.1"
                     /translation="MLKRLFVLLLALSSAAFSAGCGDSRSHAQAVYVLIDTSGTYALE
                     ANKAQMIINYLLGTLQPGDSLAVARVESRSFTEKDIIAKATFDSRPSQANAQKRAFRD
                     KTDAFLKTVKASRYTDITGGLLQGAEFLNETGAGTKTIVVFSDMQEEIDKLTVRNIPM
                     NLKRIRVVAVNVVKLKTDNIDPRRYFGRLEAWQKRVVAAGASEWRVINDLERLDTIFS
                     RT"
     gene            complement(9934..>12272)
                     /locus_tag="VFR57_03200"
     CDS             complement(9934..>12272)
                     /locus_tag="VFR57_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258412.1"
                     /translation="LVHELFHVVHLDMARGAPLGLRSLFGRLPFLFPNVFQPGWIIEG
                     LAVHKESDPARAYGRLGQAHFEGMMRAEASRGLRSLAEINAGGRRFPLNRDYLYGGYF
                     FLFLEERYGAATLASFIESFSDNVIPFRVHTNPEVPTGKTMDHLWEEYDSWLKARFTR
                     PASAAAQGEVVATGWSLTAPTLAADGTRWYVTGDGYTRARLMRQPPGGKPEALRKVEY
                     DTRLTASAAGALVLMQPEICRNYNYYYDIEALGNRVTECGRFRQAAALDDGRVVGVRL
                     EAGDAEVAVVGGEVLYRAAPGESITGVAAGAARIAVTTLRAGLWSLLLIQDGKAEVVL
                     SDAAIKHSPRIGDRDEVFFVAGYGKVYNVWSVGAGRLARWTDAAHGVREISAPYRGEL
                     LLTTIEPDGDALRSYRLPGAPLETRPLESVAQAAETQVPAMELADRPYSPWSSLAPRW
                     WFPTIELAEGAVKLGATTSGQDALALHQYVVTAQYEFTQGELLGNLAYIYNGRHLLFL
                     DRSMRVRETVNDEIEAYTIFEDAQWVSTWRHVALNRRLFWGFGGALEREELHRRNVTA
                     VEFQDERVLGLVAGIDTRREHWLSEGPSEGTQLRLFAENSYGFYSGQVYRADWRLHMP
                     AWRTVLALRWNEGWGEPQAEPFQLGGSDSDPGLIVPILNQRDFPLRGYSSGEPTLIGH
                     RSRLVSLEWRVPLRDIDRHAMVPPVGINRVSANLFFDVGDAWPRGGTPDYHRGFGVEL
                     RSEIRVGYFLPLDLRLGFAKGRDEGGDSVAYLRLGRAF"
BASE COUNT         2224 a         3915 c         4009 g         2124 t
ORIGIN      
        1 ctcgaggatg tcgtcgaagt actgcttgat cgggaaaact ttgcccttgg aggcgaggtc
       61 ctgcggcgtc acgtcgacga agagcttgtt ccaggtcttg tcgtcgagct gcgccttcac
      121 gtgctgggcg tcgatccccg gataccagtt gaacttcttc acgatgcctt cggcctggat
      181 ctgcgggctc gtcgccatct cgacgaactt cttcgccgcg gccatgttcg ccgctttggc
      241 cgggatgacg tagtacatcg gctgacccgg catgccgggg ccgatgagct gcaggcgcat
      301 gttgggcggg agctttccgt cggccatcca ggtgtagaac atgtcgaccc agaccgggcc
      361 catcgcgatc tcgccgcggt tgagcatatc cagcgtgcct gcgttacccg gggtaatgac
      421 tacgctgcgg ttgaactcct tcagctttcc gagcgccgga tcccaattcg ccttcgtctg
      481 cgcgtcgtag ggtcccttca tcagcttgtc ggaatcgggc gtattggcgt acacccagcc
      541 caccacgaaa gcgacgccgg acattccgcc cttgatgccg ttgtagccga acttgcgtgg
      601 attggctttg gcccaggcgg cgagctcttc gtaggtcttc ggcggattct tgacgagcgc
      661 ggggttgtag gcgatcgcgg tctggctctg gaacatcggg atgacgtagc cggccacgtt
      721 tgtgccgagc gcgttcttgg atgcttcgga ggtgaccagc ctgcccgtgg cgacgtcgcg
      781 cacataaggc gcaagcaatc cgtcgcgcac gagatcgccg gccatcttct gatggatcac
      841 agcgacgtcg acatcccacg ccgggttgtt cttctgcgcc tggattttct cggcgatctt
      901 ctgcgagccg gcgtcgcccg gcccggtacc gacggctttc acgcttacgt tcgcgttgtt
      961 cttctcaaac tgcggcgcga gaaaatcctt cacgtaatcg accatgttct ggtcaccggc
     1021 ggtgaccacg ttgaggttga tcttctgcgc cgccgccggc gtcgcggcga aagcggccgc
     1081 cagcgcgatc agcaccagac tgcgtttcat gtgttcctcc tcggaaagaa gtgcagcgaa
     1141 gataccggca gcccgatccc caccaactcg ccgaccgtca ggcgccgggt gtcgtccacc
     1201 atgaagtgat tttgccccac cgcgaccgcg tagcggtaaa aaccgccggg atacgaggct
     1261 tgcacgatgc ggccccgcag caacagccgg tctggcgctc cttcctgggg cgggcctaga
     1321 cgcgccgctt cgcttcgaaa gtgcgcgatc agcgggccgg ccgagcggaa gccgggcacc
     1381 cggaccgcgt cgttatgagc gccggcctgg atttccacgc cgtccgacgc cggccgggcc
     1441 tcgagctcga gcacattccc ggcccccatg aaggccgcga cgaaaggtga gttcggccgg
     1501 ttgtagacct cctccggcgt gccgagctgc gcgatccgac cggcatcaag gatggcgatg
     1561 cgatcggcca tgaccatcgc ctcttcgcgg tcgtgcgtga catggatcgt ggtgatgccg
     1621 agcttggcct gcagcgcttt gatttcgtgg cgcacttcct cgcggatgcg cgcgtcgagg
     1681 ttagagagcg gctcatccag aagcaggatc tccgggctta tggcgagcgc ccggccgagc
     1741 gccacgcgct ggcgctgccc gccggagagc tgggtcacgt tgcgctcgcc gaagccgtcc
     1801 agtttcagca tggtgagtat ctcggcgacg cgcctggcga tgtccggcct tgcgacgcgg
     1861 cgcagcttca agccatagcc caggttctgc gccgtggtca tgtgcggcca gagcgcgtag
     1921 gactggaaca ccatcgccat gccgcgcttg tccggcggcg catgcgtgat gtctcgtccg
     1981 gcgatcgaga tcttcccggc gttcaccggc acgaagcctg aaaccgcgcg cagaagcgtc
     2041 gtcttgccgc agcccgaagg tcccagcaac gcgacgaatt cgccagcggc gaccgaaagg
     2101 gagacttgat ggagaaccgg cgtcgcgccg taggagacct cgagctgcgt gatatcgagc
     2161 gccgcgcttg ccatgcgaaa cgattatggc gtaatggtgg tcctcaaagg aggtcatgta
     2221 tgcgcctctc catcgcaacc gtcagcttgt tgctagcaac gggctgccac accactgccg
     2281 agcagccagc gcgagccaaa gagccaacgc aagtgatcaa gcccgatcag gacggcgtgc
     2341 agcgtgcccg catcctcgcc ggcggctact tcttcaagcc gagccacatt gtcgtgaagg
     2401 tcaacacgcc ggtggaaatc ctggcgagcc gggaaagcgg catggtgccg cacgatctcg
     2461 tgatcgacgc gcccgaggcg ggcatcgccg tgaagcagga ccttgccacc gagccgcgca
     2521 agatcgcgtt cacgccaaag agagtcggaa gctatgccat ctattgcagc aagaagccgc
     2581 cgctgggcgg tgcgagccat cgcgagcgcg gcatggaagg tgtgctggaa gtcgttcctt
     2641 agcggcctga gaaacgcatc agcgtttgcg cataggccgg cgcagtcggc tcgtgcatga
     2701 agtacacgta gatctgctgc catcccgtct cgtcgagccg tcgcgcccat cgctcgagat
     2761 cctggtcgga ataggtttcc agacgaagac gcacgtagcc ccacggcgcg gtctcgacga
     2821 gcgggggcgg ttcgttgtcc tcccgctccg agtggcacag cgacgcgcct ctcgctttca
     2881 gggcggcgta gacctcgtcg tcgaaccagg tgtcgttgcg gaattcgaat gcagcctggt
     2941 gtcccgaagg caggaccgca aggaagtcgg tgagccgcgc gaggtccttc ttcagaaacg
     3001 gcggcagctg atagagcacc gggccgcgct tatcgccgag cgtggcgaga ttgcggtaga
     3061 ggtactcgac cgagtcgcgc gccttttcgg cctcgaggcg cgagaagtgc gtgatgcggc
     3121 gcgacgcctt gatggcgaag cggaagctct ccggcgtttg ccgtgaccag ttctccagaa
     3181 ccgtggcctt cggcatctgg tagaaggtgt tgttgatctc caccgtcggc agccgctcgg
     3241 cgtagaacgc gagcatgccg tccggcttca tgcgctccgg atagaaacgg cccttccact
     3301 ccttgaacga gtagccgctc gccccggcga gcactttctt agaattgggc atggcgaaaa
     3361 gcaagcttag cgagtattcg gcaaagcgca gcttcaccgc gacgccggag cccgcgccgg
     3421 cggtgccgga ggcgcgccga ggaccgctcc tcttcgtcat ccagcagcat tccgcgcgcc
     3481 gcatgcatta cgactttcgc ctcgagtgcg acggcgttct caagtcgtgg gccgtgccga
     3541 aagcgccgtc gctcgatcca tcggaaaagc ggctcgcggt gcagaccgag gatcatccgt
     3601 tcgattacgc gtcgttcgaa ggcgtgatac cgccgaagca atacggcgcg ggcgaggtaa
     3661 tcgtctggga ttgcggcgtg tattcgcccg acgaggccag ctcttgggtg gaggatcggg
     3721 acgaggccga gcgccaggtt cgtgaggggt tggagaaagg caagttgagc gtcttcctgc
     3781 gcggggagaa ggtgaagggc tctttcgcgc tcgtgcgcac cgcagacaag aagaactggc
     3841 tcctcatcaa gcacaaggac cgcttcgtcg gcgcagccga tctcacgacg ctcgagcgct
     3901 cggtcctgtc gggcacttcg gtggaggaaa tgaagtcggc gccggcgcat caaatgccgg
     3961 cggcacatct cattcccgtc ggtgtcgaag agcgcatgcc gaagtcgctc gcgccgatgc
     4021 atgcggagct caaggaggtg ccgttcaacc atcccgactg gatgtgggaa ccgaagctcg
     4081 acggctatcg cgtgctcgcc ttcgtcaatg acggcgtgag cttgcgttcg cggcgcggcc
     4141 tggagctcgc cgcgcagttc ccgagcctcg ccaaggagct ggcggagcag gggcccgcca
     4201 tgatcctcga cggtgagctc gtcgctttcg ataacggcaa gccctccttc aatgcgctcc
     4261 agaaccggtt tcagctgaag aacgagcgcg acatcgcggt tgccgagcgc acgtttccgg
     4321 tggttttcta ctgcttcgat ctccttcact tcgctggcat gaacctgcgc ccggctagct
     4381 atgcggaccg gcggcgctac cttgcgcagt gcctgctgcc gtcatccggt gttcagctcg
     4441 ttcatgccgc tgacgatggc gtggagctct acaaggctgc gctcgccgcc ggcttcgaag
     4501 gcgtcatcgg aaagaagcgg cagagcaagt acgaacccgg caagcgctcg gccgcctggg
     4561 tcaagatcaa atcaacgcga agcgccgagt ttgtcgtcgg cggttacacg aaagggaaag
     4621 gcgcgcgtgc gccgctcggc gcgctgctgg tcggctactg ggatgggccg aagctccact
     4681 atgcctcgca cgtgggctcg ggtttcgatg accacaacct ggacgccgtg caggcgaggc
     4741 tggagcccct taggcgcaag acgtgtccct tcgcggggaa accggagctg cacagtccaa
     4801 cagtgtgggt cgagccagag accgtcgcgg aggtgaagtt ccaggagtgg acgaaggacg
     4861 gcttcctgcg cgcaccggtg ttcctgcggc tgcgcgacga catcgatccc aaaaccgtga
     4921 agcgcgccgg cgggggcgag gaggcggcgg tgccgcgctc cgagatcgag gacgtcgtag
     4981 cccagcttgc ctcaatcaag gcgaatggga cgatcgccgc cggcaatcac acgatcaagt
     5041 tgagccacct cgaccgcgtc tactggccgg ccgacccggc gctcaagcag ccggccctca
     5101 cgaagcgcga tctcctgcgc tatctcgcgc aggtttcgcc gtacatgctc acgcacctcg
     5161 ccgatcggcc gctcaccatg atccgcatgc ccgacggcat ccacggcccg cgcttctatc
     5221 agaagcactg ggcgcaggag aagccggcgt tcgtcgatgc gatcacggtg ttttccgagc
     5281 acaaagacga aagccacgag tacctgctgt gcaacaacct gccgacgctt ctatggctcg
     5341 cgcagtcggg cacgctcgaa ttccatgtca tgcactcgcg cgcgaagctg ggcgacgatt
     5401 cgccagtgaa aagcacggac tacgcgactt cactcgacac gctcgaagga tcgatcctga
     5461 actaccccga ctatgtcgtg ttcgacatcg acccgtacat ctactcaggg aaggaaaagg
     5521 cgggcgacga gccggagctg aacacggtgg ccttcgagaa gggcaaggaa gtcgcgttct
     5581 ggctgcgcga gctgctgaat ggcatgtcgc tcgagccgat cgtgaagacc tcgggaaaga
     5641 ccggcctgca cgtcttcgtg ccgatccggc gcaccatcga tttcgacgcg gcgcgcgcag
     5701 tgtccgagct cgtcggccgg cacctcgtgg gcaagcatcc gaaggacatc accatggagt
     5761 ggagcgtacc caagcgcacc ggcaagatct tcatggacta caacatgaac gtgcgcggca
     5821 agacgctgaa tgtggcctat tcgccgcgcg gcgcaccggg cgccgcggtc tcgatgccgc
     5881 ttgcttggga cgagctggcg cacgcgcatc cgctcgactt tcgcatcacg aacgccgccg
     5941 agcggctcgc gaagaccggg gaccgctggc gcgacgcgct cacggcgaag aaaagcctgc
     6001 aaggcgccct agaaggcgcc aaaacgtaaa aatctctctg tattacaatc gcttcccttc
     6061 ttcggaggcg acatggcggc gcgctcgatt gcttcgttga cggtgtcttt cggcctggtc
     6121 tcgatcccgg tcaagctttt ttccgcaacc gaggcgagcc gggcgatctc cttcaacctg
     6181 ctgcacaagg cgtgcggctc gcgcctgcgc cagcagtact tctgcatcaa ggaagaagtt
     6241 cccgtgcagc gcgaggacat ggtcaagggc tatgaattcg cgaaggacca gtacgtcatg
     6301 ttcacgcccg aggagctcaa ggctctcgag gaggccggca ccgggaccgc ggacatcagc
     6361 gagttcgtgc cgctggaatc ggtcgatccc gtgtacttcg acaaggcgta ttacctcgcg
     6421 cccgacaagg gtggcgcgaa gccctatgcg ctcctcacca aggcgctgcg cgaatcgaag
     6481 cgttgcgcgc tcgggcgctg ggcggcgcgc ggcaagcagt acattgtcat gatccgcgct
     6541 gtgaacgagg ggctggtcat gcagcagctc atgtacgccg gcgaggtgcg ctcgatccgg
     6601 gacatcgaca ttccgaagac cgaggtgcgc gacgccgagc tcaagctcgc gcagcagttg
     6661 atcgagcagc aggcctccga caaattcgat cccagctcct ataccgatga ggtacgcacg
     6721 cgcatcgagg ccgccgtgca gaagaaggtg gaggggcagg agatcacctt ggccgaggcg
     6781 ccgcgcgaag gcgcgcaagt gatcgacctc atggaagcgc tgcgtgcgag cctcgagaag
     6841 aagcccgcga agaaggcggc gcccgcggcg aaaccggcgg cgaccaggaa agcagccaag
     6901 aagtagcgtg caacagttcg gggtgcggga ggtcgagaag ctcctgcgcc ttccgcgcag
     6961 gaccattcac ggcctcgtca aagcggggtt cgtctcgccc gcgcgcggtc cggggcagac
     7021 gtaccgcttt tcgttccagg atctgatcgt gatgcgcacc gcgcaggcgc tggctgcggc
     7081 gaacgtccca gcgaagcgca ttacgagctc gctcaaggaa ctgcgccggc acctacctgc
     7141 gtcgatgccg ctttcgggcc tgagcatcgg cgcggtcggc gatcgtgtcg tggtgaagga
     7201 aggcgcaagc cgctggcagg ccgagtccgg ccagtatctg ctggcgttcg agggcgaccc
     7261 ggccgccggc gagctcaagg tcatgcagcg tgagcccgat aacgtggaag acgagatcga
     7321 ccgcggctac gccttgcatg aagccgggaa gctcgccgag gcggaagcgg tgtaccgcga
     7381 tgcgctcgaa acgtggggac ccgacgcgct cctcttctac aacctcggcg tcctgctcga
     7441 ggatctcggg cgcaaatcgg aggcgttgca ggcttaccag gccgcgctcg gcgccgatcc
     7501 gtacatggcc gattgccact acaacctcgc gctccttttc gatgagctcg gccgggggca
     7561 ggacgccatt cggcacatgg cgcagtaccg tcgcctcgtc gcgaaaaagt aacggcaaaa
     7621 ttcacagcgc gaccccagca accgagacat agtcctcgaa gcgcccgagt tagtttccag
     7681 cgcccgccgc gactgatcta ctctcccgag gaagggagaa cgacatgtct gaaaatcaac
     7741 cctggatgct gtttcctgac aatcccgcgc tttcgatctt cgtgctcgcc atcgtcgcgc
     7801 tcgtctttct gtacgccgcg cgccgtccga tgcacgaact gctgcgcgcg ctcggtgcgc
     7861 tcttgggtgg gccgctgcgg cttggcgcgc gctggctcgt gaccacgtcc gagcagatga
     7921 aggaacgtaa ccgcgcggtg ctgctcgcgc acgggaagca cgaggtcggg cagcgcatcg
     7981 aacgcgagtt cgagcgcctc ggcgcggtgg tgcgccgcga cctgcagggt tatccgactc
     8041 tgcagagaaa gcttctcgac gagatcaccc gcatcgaaga ggactacaag aagtgcggcg
     8101 aagtgccgcc gccgccgccc gactgggtcg aagcggtcac cgccatggcc gaagtcaaga
     8161 ccggaaacga gatcgtccag aggatcctcg aggagatcaa gacctcgatc ggcaagatcc
     8221 acgacaaggc aatcagcgaa taccgcaagt cgtacgagtc gcgccacaag attctcgaga
     8281 gcttcatgcc gttctggcgt tcggtcgacc gcaacatcgc cgaagtgaac aagaagctgg
     8341 gcgacctgca ggaaagcgcg aagggcgtgg atgccctgat ggcgaaatac gaagcgatca
     8401 acgcgaagac cgacaaggcg cagcatgcgc tgaccgtttc cgcattcacg cagttcgcca
     8461 tcgcgttgct ggtcatggcg gtcgccgcgg gcggcgcgtt catcaactac aagctgatcg
     8521 cgctgccgat gtccgagatg gtcggcgccg gcgactacct caccgccagc ctgcgcacct
     8581 ccgaagtcgc cgcgctcgtc attatcttcg tcgaggcctc catggggctc tttctcatgg
     8641 aagcgctgcg catcacgcat cttttcccgc gcatcgcgac gctcagcgac catatgcgcc
     8701 ggcgcatgat gtggatcgcg ctcgtgctgc tcgtgacgct ggccggcgtg gaggccgcgc
     8761 tcgcgctgat gcgcgacatg ctgatcgcgg acaagcaggc ccttatccag tcgctcgcga
     8821 ccgtgcagca ggcggcgccc gccgacggct gggtcggcaa gattcccacg gccggtcaga
     8881 tgctgcttgg cttcatcctg ccgttcgcgc tcgcgttcat cgcgatcccc gtggaatcgc
     8941 tgatccactc gtcccgcacg gtgggcggcg tgctgctcgc cgcactggtg tccgcgcttg
     9001 cgctcacgct gcgagtgttg gccaacgtgg tgcgccgcat gtcgaaagtg ctcattaccg
     9061 tgtacgacgt cgtgatcgtg ctgccgctca tggtcgagcg catgaccaag gccggcatgg
     9121 cgatgcgcca ttcggcggtc gacgccgacg ataccgccta caagacgagg tagccatgct
     9181 caagcgactc ttcgtgttgt tgctcgcgtt gtcgtcggca gcgttctccg ccggctgcgg
     9241 ggactcgcgc agtcacgcgc aggcggtgta cgtgctgatc gacacctcgg gcacctacgc
     9301 gttggaagca aacaaagcgc agatgatcat caactacctg ctcggcacgc tccagcccgg
     9361 cgattcgctg gcggtggcgc gcgtcgaaag ccgtagcttc acggagaagg acatcatcgc
     9421 caaggcgacc ttcgactcgc ggccgagcca ggcgaacgcg cagaagcgcg ccttccgcga
     9481 caagacggat gcgtttctga agaccgtgaa ggccagtcgc tacaccgaca tcaccggagg
     9541 ccttttgcag ggcgcggagt tcctgaacga aaccggcgcc ggcacgaaga cgatcgtcgt
     9601 gttctccgac atgcaggaag agatcgacaa gctcacggtg cgcaatattc cgatgaacct
     9661 gaagcgcatc cgggtcgtcg cggtcaacgt ggtgaagctc aagaccgaca acatcgaccc
     9721 gcgccgctat ttcgggcgcc tggaggcgtg gcagaagcgg gtggtcgcgg cgggcgcgtc
     9781 ggagtggcgc gtgatcaacg atctcgagcg cctcgacacc atcttctcgc gcacctaaga
     9841 accttcttcg tcgcccccgc gaaggcgggg gcccagtttt cttttctttg aatatgggtt
     9901 cccgctttcg cgggaacgac actctgcgtc ttactaaaag gctcgcccga ggcgaaggta
     9961 ggcgacgctg tcgccgcctt cgtcgcggcc cttggcgaag ccaaggcgca ggtccagggg
    10021 caagaagtac cccacgcgga tctccgagcg cagctccacc ccgaaaccgc ggtgatagtc
    10081 cggcgtaccg ccccggggcc acgcgtcgcc cacgtcaaag aagagattgg ccgacacgcg
    10141 gttgatgccg acaggcggca ccatggcatg ccggtcgatg tccctgagcg gcacgcgcca
    10201 ttccaggctc accaggcgcg aacggtggcc aatcaaggtg ggctcgccgc tgctatagcc
    10261 gcgcaacggg aaatcgcgct ggttgaggat cggcacgatc aagccgggat cggagtcgct
    10321 gccaccgagc tggaacggct cggcctgcgg ttctccccag ccttcgttcc agcgcagcgc
    10381 gagcaccgtg cgccacgccg gcatgtgcag ccgccagtcc gcccggtaga cctgtccgga
    10441 gtagaagccg taggagttct cggcgaagag tcgaagctgc gttccctcgc tcggtccctc
    10501 cgaaagccaa tgctcgcgcc gcgtgtcgat gccggccacg agaccgagca cgcgttcgtc
    10561 ctgaaactcc accgcagtca cattgcgacg gtgcagctct tcgcgttcca acgcaccgcc
    10621 gaagccccag aagaggcgcc ggttgagcgc gacatggcgc cacgttgaaa cccactgcgc
    10681 gtcctcgaaa atggtgtagg cctcgatctc gtcgttgacg gtctcgcgca cgcgcatcga
    10741 gcggtcaagg aagagaaggt gccggccgtt gtagatatag gcaaggttgc cgagcagctc
    10801 gccttgggtg aattcgtatt gcgccgtgac gacgtactga tgcagggcga gcgcgtcctg
    10861 gccggatgtg gtggcgccga gcttgaccgc tccctccgcg agctcgatcg tcgggaacca
    10921 ccagcgcggc gcgagcgagc tccaggggct gtaggggcgg tccgcaagct ccatggcagg
    10981 gacctgcgtt tcggcggctt gtgcaaccga ttccaatgga cgcgtttcca gcggcgcgcc
    11041 gggcaggcga taggagcgca acgcgtcgcc gtcgggctcg atcgtcgtga ggaggagctc
    11101 gccgcgatac ggcgcgctga tctcccgcac gccgtgcgcg gcatccgtcc agcgcgccag
    11161 ccggccggcg ccgacggacc acacgttgta gaccttgcca tagccagcga cgaagaaaac
    11221 ctcgtcgcgg tcaccgatgc gcggcgaatg tttgatcgca gcgtccgaca gcaccacttc
    11281 cgccttgccg tcttggatga gaaggaggga ccacaggcct gcgcgcagcg tggtgacggc
    11341 aatgcgcgct gcgcctgcgg ccacgccggt gatggattcg ccaggcgcgg cgcgatacaa
    11401 gacttcgccg cctacgacag cgacctccgc atcgccggct tcgaggcgca cgccgacgac
    11461 ccggccgtcg tccagcgctg cggcctgtcg aaaacgtccg cattcggtga ctcggtttcc
    11521 gagcgcttcg atgtcgtagt agtagttgta gttgcggcag atctcgggct gcataaggac
    11581 gagcgctccg gcggccgatg cggtcaggcg cgtgtcgtac tccaccttgc gcaatgcttc
    11641 aggctttccg cctgggggct ggcgcatgag ccgggcgcgc gtatagccat cccccgtcac
    11701 gtaccagcgc gtgccgtcgg cagcgagcgt cggcgcggtc agcgaccagc cggtcgcgac
    11761 aacctcgcct tgcgccgccg cggaagctgg gcgcgtgaag cgggccttga gccaggagtc
    11821 gtattcctcc cataggtggt ccatggtctt cccggtcggg acctcgggat tggtgtggac
    11881 gcgaaaggga atgacgttgt cgctgaagct ttcgatgaac gaagcgagcg tcgcggcgcc
    11941 atagcgctcc tcgaggaaga gaaagaagta accgccgtag aggtaatcgc ggttgagcgg
    12001 gaagcgccgg ccgccggcgt tgatttcggc gagcgagcga agcccgcggc tcgcctccgc
    12061 gcgcatcatg ccttcgaaat gcgcctgtcc gagccgcccg taggccctgg ccggatcgga
    12121 ttccttgtgc accgcgagtc cctcgatgat ccagccgggc tggaacacgt tcgggaaaag
    12181 aaacggcagg cggccgaaca ggctgcgcag gccaagcggc gcgccgcgcg ccatgtcgag
    12241 atgcacgaca tggaagagct cgtggacgag ca
//
LOCUS       DASTPD010000091         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_249959, whole genome shotgun sequence.
ACCESSION   DASTPD010000091 DASTPD010000000
VERSION     DASTPD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249959"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFR57_03205"
     CDS             complement(<1..569)
                     /locus_tag="VFR57_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0258413.1"
                     /translation="MERIQDGQLAMMPIEFKLNGETVVGRPDEPIITTAKKHGVDIPH
                     LCHGGNQRSDGNCRACVVEIKGERVLAPSCCRFPTQGMEVTTNSPRALTSQKMVLELL
                     LSDMPEQEYTLDNRLKQWAQRLGVGKPRFEKREQPKADLSHPAIAVNLDACIQCTRCV
                     RACREVQVNDVIGYAGRGEHSKIVFDFDDP"
     gene            complement(585..2237)
                     /locus_tag="VFR57_03210"
     CDS             complement(585..2237)
                     /locus_tag="VFR57_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU0258414.1"
                     /translation="MKTRVRPILKGRAVDLKALQDVRELLVGAPRRRDLLIEHLHRIN
                     DRYGYLGAAHLAALAQEMSLALTEVYEVATFYHHFEVVKEGERAPAALTVRVCDSIAC
                     ELAGARELLKGLKFGDEVRVIPAPCVGRCEVAPCVVVHQNPVANATLDLVKRAVERKQ
                     LSCAETSYIDYAQYRKAGGYKLIADCLAGKRTREEITKMMEDSGLRGLGGAGFPAGRK
                     WRLVAAEPEPRVMAVNIDEGEPGTFKDRWYLERDPHRFLEGMLVAAWCSGVGEIWIYL
                     RDEYAGCRAILDRELKKLQAEKPCALPPMHLRRGAGAYICGEESAMIESIEGKRGMPR
                     LRPPYVAQVGLFGRPTLEHNMETVYWVREIVEKGPQWFAAQGRNGRKGLRSFSVSGRV
                     KKPGVHLAPAGITVLQLINEYCGGMLEGHELYAYLPGGASGGILPASKADIPLDFDTL
                     QPHGCFIGSAAIVVLSDKDKASTAAVNLMKFFHDESCGKCTPCRVGTGKAVALMEQAK
                     WNQPLLNELSQAMMDASICGLGQAAPNPALSVMKYFPHEIAG"
     gene            2306..>2946
                     /locus_tag="VFR57_03215"
     CDS             2306..>2946
                     /locus_tag="VFR57_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0258415.1"
                     /translation="MKYRLLGRTGVQVSQLCFGTMSFGGDADPQESARMYRACRDAGI
                     NFFDTADQYNKGRSEEILGELMRGERENLVIATKCFNPTDADINARGSSRRHVSRALE
                     ASLKRLGTDRIEILYLHHYDALTPLEEQARALEDLVRAGKVLYPALSNFAAWQTQAAL
                     GVQERGGWARLQAMQPMYSLVKRQAEVELLPMAQANGLAVFPYSPAGGGLLSGR"
BASE COUNT          480 a          974 c          943 g          549 t
ORIGIN      
        1 gggtcgtcga agtcgaacac gatcttcgag tgctcgccgc ggccggcgta gccgatgacg
       61 tcgttcacct gcacttcacg gcaggcgcgc acgcagcgcg tgcactggat gcacgcgtcg
      121 aggttcacgg cgatggcggg atgggacaga tctgctttcg gctgttctct tttctcgaat
      181 cttggcttgc caaccccgag gcgttgcgcc cactgcttga gcctgttgtc cagggtgtac
      241 tcctgctccg gcatgtcgga gagcagcagc tcgagcacca ttttctgcga agtgagcgcg
      301 cgcggagaat tggtggtcac ttccatgccc tgcgtcggga agcggcagca cgagggcgcg
      361 agcacacgct cgcccttgat ctccactacg caggcgcggc agtttccgtc cgagcgctgg
      421 ttgccgccat ggcacaggtg cgggatgtcg acgccgtgct tctttgccgt ggtgatgatc
      481 ggctcgtcgg gacggccgac cacggtctcg ccgttcagct tgaactcgat cggcatcatt
      541 gcaagctggc cgtcctggat acgttccatg gtcagagagc ctttctaccc tgctatctcg
      601 tgcgggaaat acttcatgac gctaagggcg ggattcgggg cggcttgacc caggccgcag
      661 atcgacgcgt ccatcattgc ctgtgaaagc tcgttgagca gcggctggtt ccatttcgcc
      721 tgttccatga gcgccaccgc cttgcccgtt cctacccgac aaggcgtgca cttgccgcac
      781 gactcgtcgt ggaagaactt catgaggttg accgccgccg tgctcgcttt gtctttgtcg
      841 gacagcacga caatcgccgc cgagccgatg aagcagccgt gcggctgcag cgtatcgaag
      901 tcgagcggga tgtccgcctt cgacgccggc aggatacctc cggaagcgcc gccgggcagg
      961 taggcgtaga gctcgtgacc ctcgagcatg cccccgcagt attcatttat tagctgaaga
     1021 accgtaatgc ctgcgggagc gaggtgaact cccggctttt tcacgcggcc ggagaccgag
     1081 aacgaacgca ggcccttgcg gccgttgcgc ccctgggcgg cgaaccattg cggccccttc
     1141 tcgacgatct cgcgcaccca gtacaccgtc tccatgttgt gctccagggt cggccggccg
     1201 aagagaccta cctgcgcgac gtaagggggc cgcaggcgcg gcatgccgcg cttgccttcg
     1261 atcgactcga tcatggcgga ttcttcgccg cagatatacg cgcccgctcc tctacgcagg
     1321 tgcatcggag gcagggcgca tggcttttcc gcctgcagct tcttcaattc cctgtcaaga
     1381 atcgcgcggc agcccgcgta ttcgtcccgc aggtagatcc agatttcgcc cacgccggag
     1441 caccaagcgg cgaccagcat cccttccagg aaacggtgcg ggtcgcgctc caagtaccag
     1501 cggtccttga acgtgccagg ctcgccttcg tcgatgttga ccgccatgac gcgcggttcg
     1561 ggctcggccg cgaccagccg ccacttgcgc cccgccggga agccggcgcc gcccagtccg
     1621 cgcagcccgg agtcctccat catcttggtg atctcttcgc gcgtgcgctt gccggcaagg
     1681 cagtcggcga tcagcttgta gcctccggcc ttgcggtact gcgcgtagtc gatgtagcta
     1741 gtttccgcac aggacagttg ctttcgctcc acagctcgct tcaccaggtc caacgtcgcg
     1801 ttggcgaccg ggttctgatg cacgacgacg cagggcgcga cctcgcagcg gccgacgcac
     1861 ggcgccggaa ttacgcgcac ttcatcgccg aatttcagtc ccttgaggag ctcgcgggcg
     1921 ccggcgagct cgcaggcgat cgagtcgcac acccgcacgg tcagcgcggc gggcgcgcgc
     1981 tcgccttcct tcacgacttc aaagtgatgg tagaacgtcg ccacctcgta gacctcggtg
     2041 agcgcaagcg acatctcctg tgcgagcgcc gcgagatgcg cggcgcccaa gtagccgtag
     2101 cggtcgttga tgcggtgcag atgctcgatc aggaggtcgc ggcggcgcgg cgcaccgacg
     2161 agcagctctc gcacgtcttg caacgccttc agatcgacgg cgcgaccctt gagaatcgga
     2221 cggactctgg ttttcatgcc cagcgcctat tatcgggcag gggtgcgcac ggttttcttg
     2281 actgcggtca aggtagactt tccggatgaa atatcggctg ctcgggcgca ccggagtcca
     2341 ggtttcccag ctttgcttcg gcacgatgtc cttcggcggc gatgccgatc cccaggagtc
     2401 ggcgcgcatg tatcgcgcct gccgcgatgc gggaatcaac ttcttcgaca ccgccgacca
     2461 gtacaacaag gggcgttcgg aggagatcct cggcgagctc atgcgcggcg agcgcgaaaa
     2521 cctggtcatc gctaccaagt gcttcaatcc gaccgacgcc gacatcaacg cgcgcggatc
     2581 gtcgcgccgc catgtcagcc gtgcgctcga ggccagcctg aagcgcctgg gtaccgaccg
     2641 catcgagatc ctctacctgc accactatga cgcgcttacg ccgctcgagg agcaggcgcg
     2701 cgcgctggaa gacctggtgc gcgcgggcaa ggtgctctac ccggcgctca gcaacttcgc
     2761 cgcctggcag acgcaggccg cgctcggtgt ccaggagcgc ggtggctggg cacgcctgca
     2821 ggcgatgcag ccgatgtaca gcctggtgaa gcgccaggcc gaggtggagc tgctgccgat
     2881 ggcgcaggcg aacggcctgg cggtgttccc ctacagcccg gcgggcggcg gcctgctctc
     2941 cggccg
//
LOCUS       DASTPD010000092         5803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_250167, whole genome shotgun sequence.
ACCESSION   DASTPD010000092 DASTPD010000000
VERSION     DASTPD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250167"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFR57_03220"
     CDS             <1..427
                     /locus_tag="VFR57_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0258416.1"
                     /translation="EDGARARRLAGKLDRVLVDAPCSGFGTLRRNPDLKWRHDPGAIT
                     ELAAKQRNILGAAARLVKPGGRLVYATCSILQEENEAVSTQFQSEHPDFIALSCAELL
                     AAQRIELSTGEQLRLFPHRHGTDGFYAAAFAKRPFAKGA"
     gene            505..1683
                     /locus_tag="VFR57_03225"
     CDS             505..1683
                     /locus_tag="VFR57_03225"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU0258417.1"
                     /translation="MLFSGLLDLPWWGYAIAALALTHVTIASVTIYLHRCQAHRALEL
                     HPIAAHFFRFWLWLTTGMVTKEWAAIHRKHHAKVETAEDPHSPQIYGINRVLWLGVFL
                     YVKEAYNRETLERYGHGTPDDWLERNVYTPHSLPGVLLLLLANLLIFGVVPGALIWLT
                     QIAWIPFWAAGVINGVGHYFGYRSYDVPDASTNIVPWGILIGGEELHNNHHAFASSAK
                     LSTKWYEFDIGWMYICILESLGLATVKRLAPTPRFARPKPAVDLDTLHAVIANRYDVL
                     SRYAKSFRRTYAEELERLRHWSPRDAELLRSLKRALLRGQAAAGSERARLAEALKNSR
                     ALATALAMRHELAALWDRSSASKDELLRQLQDWCRRAESSGVAPLVDFSQRLRSYALA
                     "
     gene            complement(1715..1870)
                     /gene="rpmG"
                     /locus_tag="VFR57_03230"
     CDS             complement(1715..1870)
                     /gene="rpmG"
                     /locus_tag="VFR57_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU0258418.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRSTPDKLEMKKYDPKARKHVL
                     YKETKLK"
     gene            complement(1873..2106)
                     /gene="rpmB"
                     /locus_tag="VFR57_03235"
     CDS             complement(1873..2106)
                     /gene="rpmB"
                     /locus_tag="VFR57_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU0258419.1"
                     /translation="MSRVCQVTGKKPMKGHYVSHANNKTTRTFEPNLHYRRFWVETQN
                     RWVRLRVSTAGLRRIDKLGVEAVLAEIKARKKA"
     gene            complement(2156..2827)
                     /gene="radC"
                     /locus_tag="VFR57_03240"
     CDS             complement(2156..2827)
                     /gene="radC"
                     /locus_tag="VFR57_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU0258420.1"
                     /translation="MAISDWSVDRRPRERLLAKGADSLRDDELLAVLLRTGVHGKSAV
                     DLGNDLLARFGSITRLLSAGPELLEIKGLGQAKVAQFAAAIELARRAMAEELRERSAL
                     TSPGAVRDYLRVALGHRPHEVFVCIWLDAQHKVIGIEDAFQGTLTQTSVYPREIVKRA
                     LARNAAAVIFAHNHPSGVAQPSHSDELLTRDLKEALALVEVKVLDHFIVAGNQAISFA
                     ERGLL"
     gene            2878..4089
                     /gene="coaBC"
                     /locus_tag="VFR57_03245"
     CDS             2878..4089
                     /gene="coaBC"
                     /locus_tag="VFR57_03245"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0258421.1"
                     /translation="MGLTGKRILLGITGGIAAYKAAELTRLLVKDGADVRIAMTEAAT
                     HFIGAATLQALSGQPVWTDLWDARVNDAMGHIELSRDRELIVVAPASADFMAKLAHGL
                     ADDLLSTLCVARRCSLMVAPAMNVEMWQNPATVRNAETLRGDGIQIVGPASGGQACGE
                     MGMGRMSEPADILADIRFFFQPQRLEGKLRGKRVVVTAGPTEEPIDPVRILTNTSSGK
                     MGYAVARAAREAGADVTLISGPVSLPTPVGVSRVDVRTAREMFDAVKKDAKDADIFIA
                     VAAVADYRVRNPASQKIKKGNGHLSLELEENPDILAWVAALPTPPFCVGFAAESENLA
                     HNARAKLAKKKLPLIVGNLAQDALGADASAITLYDASGEHPLGRGPKLELARRLVEHV
                     AGMLRQKNAVK"
     gene            4086..4535
                     /gene="dut"
                     /locus_tag="VFR57_03250"
     CDS             4086..4535
                     /gene="dut"
                     /locus_tag="VFR57_03250"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU0258422.1"
                     /translation="MRKLEVKILDERIRGMLPHYGSAGAAGLDLRACVDRELILGPGE
                     SQLVSSGIAIHVADPGYAAVILPRSGLGAKNGIVLGNLVGLIDSDYQGPLMISVWNRG
                     KSAFTIQPLDRIAQMIVVPVVQVEFEVVEEFAASARGAGGFGSTGKS"
     gene            complement(4532..5767)
                     /locus_tag="VFR57_03255"
     CDS             complement(4532..5767)
                     /locus_tag="VFR57_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0258423.1"
                     /translation="MRRRDFLKLAGAVSLAGCATPTPSKSRVVVVGGGFGGATAAKYI
                     RMWDPSIDVVLIEREAQFVSCPISNLVLGGFTGMQDISRGYDGLRRHGVQVVRDEVAA
                     IDPAKKTVRLASGSDIVYERVIVSPGIDFTFGEIQGYEAAMQAGAVLHAWKAGPQTVA
                     LRQKLEQMPDGGVYILSIPTAPYRCPPGPYERASMVAAYFKQAKPRSKVLVLDANPDV
                     TSKGALFKRAWTDLYKDILEYRGNAKVVAVETGAVRTEFDTVKGDVLNVVPPHRAADI
                     AAKSGLITTNNRWCDIDWRTMESKAVKGVHVLGDATLSAPGMPKSGSMANNHAKIAGA
                     AIVELLNGRNPQPVKIINTCYSFVGEKEAIRVSSVHEWDGSTLQPVKGAGGVSAARSA
                     DEASFAWNWARTIWADSFA"
BASE COUNT          979 a         1887 c         1889 g         1048 t
ORIGIN      
        1 tgaggacggg gcgcgcgcaa gacggctcgc gggaaagctc gatcgcgtgc tcgtcgatgc
       61 gccgtgcagc ggcttcggca cgcttcggcg aaaccccgac ctgaaatggc ggcacgaccc
      121 gggcgccatc accgagctgg cggcgaagca gcgcaacatc ctcggtgccg cagcgcggct
      181 ggtgaagccc ggcggacgcc tcgtctatgc cacctgcagc atcctgcagg aagagaacga
      241 ggcggtcagc acccagttcc aaagcgagca cccggacttc attgcgcttt cctgtgcgga
      301 actgctcgcg gcgcagcgca tcgaattgag taccggcgag cagctgcgac tttttcccca
      361 ccggcacggc accgacggct tctacgcggc cgcctttgca aaaagacctt ttgccaaagg
      421 ggcttaggaa gtggggcgag gcatggcgca ttcgcgtaaa atggcggccg ctcccccaca
      481 cataaccagg acaaacccga cctgatgctt ttttccggcc tgctggacct cccttggtgg
      541 gggtacgcga ttgccgctct cgcgctgact cacgtgacga tcgcgtcggt cacgatctac
      601 ctgcatcgct gccaggcgca tcgcgcgctc gagcttcatc ccatcgccgc gcacttcttc
      661 cgcttctggc tgtggcttac caccggcatg gtcaccaagg aatgggctgc aattcaccgc
      721 aagcaccacg cgaaggtcga gaccgccgag gatccgcaca gcccgcagat ctacggaatc
      781 aatcgggtgc tctggctggg cgtattcctc tatgtcaagg aggcgtacaa ccgcgagacg
      841 ctcgagcgtt acgggcacgg aacgcccgac gactggttgg agcgcaacgt ctatacgccg
      901 cactcgctac ccggagttct actgctcctc ttggcgaacc tgctgatctt cggcgtggtt
      961 ccgggggcgc tgatctggtt gacgcagatc gcctggattc cgttctgggc ggccggcgtc
     1021 atcaacggtg tcggccacta cttcggctac cgcagctacg acgtacccga cgcaagcacc
     1081 aacatcgtcc cctggggcat cctgatcggc ggagaggagc tgcacaacaa ccaccatgcg
     1141 ttcgcttctt cggccaagct ttccacaaag tggtacgaat tcgacatcgg ctggatgtac
     1201 atctgcatcc tggagtcgct cggcctggcg acggtgaagc gccttgcacc gaccccgcgc
     1261 ttcgcgcgtc cgaaacccgc agtcgacctc gatacgttgc acgcggtgat cgccaaccgc
     1321 tacgacgtgc tctcgcgcta tgcgaagtcc ttccgccgca cctatgctga agagctcgag
     1381 cggctgcgtc actggtcgcc gcgcgacgct gagctcctgc gctctctcaa gcgggcgctg
     1441 ctccgcgggc aggccgccgc cggcagcgag cgcgcgcggc tcgccgaggc actgaagaac
     1501 tcccgcgcgc tcgccactgc gctcgccatg cgacacgagc ttgcggcgct ttgggatcgc
     1561 tccagcgcct ccaaggacga gctgctgcgc caactgcagg actggtgccg gcgcgcggaa
     1621 tcaagcgggg tcgcgccgct cgtggatttc tcgcagcgcc tgcgctcgta cgcgctagcc
     1681 tagcaggacg aaaagcccgc cgcagcgggc tttttcattt cagcttggtc tctttgtaga
     1741 ggacgtgctt gcgcgccttg ggatcgtact tcttcatctc cagcttgtcg ggcgtgctgc
     1801 gcttgttctt agtggtggtg tagaagtggc cggtgccggc cgaggactcc agcttgatct
     1861 tttcgcgcat ggtcaggcct tcttcctggc tttgatttca gcgagcacgg cttccacgcc
     1921 aagcttatca atgcggcgca gacccgcggt ggagacgcgc aagcgcaccc agcggttctg
     1981 agtttcgacc cagaaacgcc ggtaatggag gttgggttcg aaagtgcgcg tggtcttgtt
     2041 gttggcgtgc gaaacgtagt ggcccttcat cggcttcttg ccggtgacct ggcagacacg
     2101 ggacatcggg tgctccggag gcggtttgcc tgagttgcga aaggcggcga ttctactata
     2161 acaatccgcg ctccgcgaag gagattgcct ggttgcccgc gacgatgaag tggtcgagca
     2221 ccttgacctc caccagcgcg agcgcctctt tgaggtcgcg cgtgaggagc tcgtcggagt
     2281 gactgggctg ggcgacgccg gagggatggt tgtgcgcaaa gatcaccgct gccgcattcc
     2341 gcgcgagcgc gcgcttcacg atctcgcgcg gataaacgct ggtctgcgtc agcgttccct
     2401 ggaatgcgtc ctcgatgccg atgactttgt gttgcgcatc gagccagatg cagacgaaga
     2461 cctcatgggg ccgatggccg agcgcgacgc gcaagtagtc gcgcacagca cccggggaag
     2521 tgagcgcgct tcgttccctc aattcctccg ccatcgcgcg acgcgccagc tcgattgcag
     2581 ccgcgaactg cgccaccttg gcctggccga gccccttgat ctcgagcagt tccggtcccg
     2641 cggataacag ccgcgtgatg ctgccaaagc gcgcaagaag atcgttgccg agatccacgg
     2701 cgctcttgcc atgcacaccg gtgcgcagca atacggcgag cagctcgtca tcccgcagcg
     2761 agtccgcgcc cttggcgagc agccgctccc gcggccgccg gtcaacggac cagtctgaaa
     2821 tcgccatgtc cgggggtttc ccttaaaatc gtttccccct taacgagggt cggttcaatg
     2881 gggttgaccg gaaagcggat tcttctcggc atcaccggcg gcatcgcggc gtacaaagcc
     2941 gccgagctga ctcgcctgct ggtgaaggac ggtgcggacg tgcgcatcgc catgaccgag
     3001 gcggcgacgc acttcatcgg tgccgcgact ctccaggcgc tctccggcca gccggtgtgg
     3061 acggaccttt gggatgcccg cgtcaacgac gcaatggggc atatcgagct ctcgcgcgat
     3121 cgggaactga tagtcgtcgc gcccgcatcg gccgacttca tggcgaagct cgcgcacggc
     3181 ctggccgatg atctgctgtc cacgctttgc gtggcgcggc gctgctcgct gatggtggcg
     3241 ccggcgatga acgtggagat gtggcagaac ccggcgaccg tgcgtaatgc cgaaacgctg
     3301 cgcggcgacg gcatacagat cgtcgggccc gcttccggag gccaggcctg cggggagatg
     3361 ggcatgggcc gcatgagcga gcccgccgac atcctggccg acatacggtt tttttttcag
     3421 ccgcagcgtc tagaggggaa actgcgcggc aagcgcgtcg tcgtcacggc gggcccgacc
     3481 gaggagccga ttgacccggt tcgcatcctc acgaacacga gctcggggaa gatgggttac
     3541 gcggtcgcgc gcgccgcgcg tgaagccggt gctgacgtaa cgctgatcag cggcccggtt
     3601 tcgctaccca ccccggtcgg cgtgtcacgc gtcgacgtgc gtactgcgcg tgagatgttc
     3661 gacgcggtga agaaggatgc gaaggacgct gacattttca tcgccgtcgc tgcggtcgcc
     3721 gattaccggg tcaggaatcc tgcctcgcag aagatcaaga aaggcaacgg ccacctctcg
     3781 cttgagctcg aggagaaccc ggacatcctc gcctgggttg ccgcgctgcc gacgccgccg
     3841 ttctgcgtgg gcttcgccgc agagagcgag aacctggcgc acaacgcaag agcgaagctg
     3901 gccaagaaaa agctgccgct gatcgtcggc aacctggcgc aggacgccct cggcgccgac
     3961 gccagcgcca tcacgctcta tgacgcgagc ggcgagcacc cgctcggccg cgggccgaag
     4021 ctcgagctgg cgcgcaggct cgtggagcac gttgcgggca tgctgcggca gaaaaacgcg
     4081 gttaaatgag gaagctcgaa gtcaagatcc tcgacgagag gatccgcggc atgctgccgc
     4141 attacggcag cgcgggtgct gccggcctcg atttgcgggc ttgcgttgac agggaactta
     4201 tcctcgggcc cggcgaaagc cagctcgtat cgtccggcat tgccattcat gtggctgatc
     4261 cgggctacgc tgcggtcatc ctgccgcgct cgggcctcgg cgcgaagaac ggcatcgtgc
     4321 tcggtaatct ggtcgggctc atagactccg actaccaagg gccgctgatg atctcggtat
     4381 ggaaccgcgg caagagcgct ttcacgatcc aacctctcga ccgcatcgcg cagatgatcg
     4441 tcgtaccggt ggtgcaggtg gagttcgagg tggtcgagga attcgccgcc agcgcgcgcg
     4501 gcgccggcgg tttcggaagc acagggaaga gttaggcgaa cgagtcggcc cagatcgtgc
     4561 gggcccagtt ccacgcgaag ctcgcttcgt ctgcgctgcg cgcggccgag acgccgccgg
     4621 cgcctttcac cggctgcagc gtgctgccgt cccactcgtg caccgaggag acgcggatcg
     4681 cttccttttc cccgacgaag ctgtagcagg tgttgatgat tttcaccggc tgagggttgc
     4741 gaccgttcag cagctcgacg atggccgcgc cggcgatctt ggcgtggttg ttcgccatgc
     4801 tgcccgactt cggcattccg ggcgccgaca gcgtcgcgtc gccgaggacg tgcacgccct
     4861 tcaccgcctt cgactccatg gtgcgccagt cgatgtcgca ccagcggttg ttcgtcgtga
     4921 tgagcccgga cttggcggcg atgtcggccg cgcgatgcgg cggcacgacg ttcagcacgt
     4981 cgcccttcac ggtgtcgaat tcggtacgta ccgcgcccgt ttcgactgcc accactttgg
     5041 cgttgccacg gtattcaagg atgtccttgt agagatccgt ccaggcgcgc ttgaagagcg
     5101 cacccttgga ggtgacgtcc gggttcgcgt cgagcacgag caccttcgag cgcggcttcg
     5161 cctgtttgaa gtacgccgcc accatgctcg cgcgctcgta gggcccgggc gggcagcggt
     5221 acggcgcggt ggggatcgaa agaatgtaga ctccaccgtc gggcatctgc tcgagcttct
     5281 ggcgcagcgc gacggtctgc ggccccgcct tccatgcgtg cagcacggct cccgcctgca
     5341 tggcagcctc gtagccctgg atctcgccga acgtgaagtc aattcccggc gagacgatca
     5401 cgcgctcgta cacgatgtcg ctgccgctcg ccaggcgcac ggtcttcttc gccgggtcga
     5461 tggccgcgac ctcgtcgcgc acgacctgaa cgccgtggcg gcgcaggcca tcgtagccgc
     5521 ggctgatgtc ctgcatgccg gtgaaacctc cgagcaccag attggatatc gggcaggaaa
     5581 cgaactgcgc ctcgcgctcg atcagcacga cgtcgatcga ggggtcccac atgcggatgt
     5641 acttggcggc ggtggcgccg ccaaagccgc cgccgacgac tacgacgcgc gacttggaag
     5701 gcgtgggcgt cgcgcacccg gcgagcgata ccgcgccagc gagcttgaga aagtctctgc
     5761 gtctcatcgc ggcctcctag cgcttctgcg cggcgaacca ggc
//
LOCUS       DASTPD010000093         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1678907, whole genome shotgun sequence.
ACCESSION   DASTPD010000093 DASTPD010000000
VERSION     DASTPD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678907"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFR57_03260"
     CDS             complement(<1..1448)
                     /locus_tag="VFR57_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0258424.1"
                     /translation="MLLASKTSSLAGLLVAAAVLYLARDVLIPLALAILAAFLLAPVV
                     RRLEYLRLGRIASTLVAVAVAGGLIGGIGYVAAREALSLAAKLPEYRENIREKARALR
                     APDDGALGKAAEAIKELESEAAPAAAAPLPVTETPPSAFAALAEWVGPFVKPIGTALA
                     VVVFTILMLLNRENLRERLIGLIGARRINVTTQALGEASDRLSRYLSTLLLVNACFGI
                     PFGIALHFIGVPNAMLWGLLGTLLRFIPYVGVWLAVAAPALLAFAIFDGWSEVTWVLG
                     TFLVLELILVNAIEPWLYGRSAGLSAIAVIAAALFWTWLWGPVGLLLAVPLTVCIAVM
                     GRYIPEMGWLNMLLGVEPVLTPEARLYQRLIALDDEEAMALAHEHAREHGVAALYDSV
                     IVPALSLAEGDRHAGALDPARERFVLDTAREIVEEIPHKEPAEAQCRHVCILPAHDEA
                     DDLAGAMLARLLPGAQLFTSKSLAAEMLECVGA"
     gene            complement(1432..3561)
                     /gene="glgX"
                     /locus_tag="VFR57_03265"
     CDS             complement(1432..3561)
                     /gene="glgX"
                     /locus_tag="VFR57_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU0258425.1"
                     /translation="MNAPELRLSRVREGLPYPRGATWDGKGVNFALFSANATRVEACL
                     FDSEGKERERIELPEFRDEIWHGYVPDIGPGTAYGFRVHGPYAPEEGHRFNPNKLVLD
                     PYARAHMGELKWDPAIFGYTMGAENTDLSFDSRDSAPFVPKCMVVDPNFDWARERQWH
                     PVPWDRTIIYETHVRGYTKLHPRVPEHQRGTYCGLAVKEVIAYIKSLGVTSVEMLPVH
                     TFIDDEFLLKKNLRNYWGYNSIGFFAPDPRYASDPSKTLQEFKEMVARFHDAGLEVII
                     DVVYNHTAEGNELGPTLSFKGIDNASYYRLIADNRRYYINDTGTGNTLNLSHPVVIQM
                     VCDSLRYWAEEMHVDGFRFDLGTILAREPSGFDTYNGFMKALMQDPVLAKVKLIAEPW
                     DCGPGGYQLGRFPPGWAEWNDQFRDTVRDYWRGEATPGALAPRLCGSPDIFNRRGRQP
                     WASVNFITAHDGFTLYDLVTYNDKHNEANREGNKDGHSHNRSWNHGVEGPTDDEAIRG
                     LRKRQMKNMLATLLLSQGTPMLLAGDEFGRSQRGNNNAYCQDNEISWLRWVHDDRAQR
                     LIGFVQKLLGLRSRYPILRRPRFPSGTFDAELGVKDVTWINATGEEMREEHWKDANVR
                     CFGMLLDGRAQTSGIHKRGEEATLLIIFNPWQDLVKFRLPQANGGERWKLLADTNFPD
                     SKEEPQFPFGHEYEVTARSLLLFALSQ"
     gene            3654..3944
                     /locus_tag="VFR57_03270"
     CDS             3654..3944
                     /locus_tag="VFR57_03270"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU0258426.1"
                     /translation="MAGWDRFVNSPPGRRFQDRYRRKRRDKGSGWKRCALVCGGIALA
                     LVGVFFMAVPGPGIPILAIGLALVAQESAAMARLLDRTELRLRRLWKRVRKR"
     gene            complement(3941..4066)
                     /locus_tag="VFR57_03275"
     CDS             complement(3941..4066)
                     /locus_tag="VFR57_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258427.1"
                     /translation="MHELWLKAVWVAVFAFVSATAFAAYLKPEMLVEFANLVLCS"
     gene            complement(4047..>4637)
                     /locus_tag="VFR57_03280"
     CDS             complement(4047..>4637)
                     /locus_tag="VFR57_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258428.1"
                     /translation="AMRERGFHLLFWGYFVCGFHIAMLTVHLPAFVTDAGLSATHGMT
                     ALALIGLFNVVGTLGAGWAGGRFSKKYLLSTIYAVRAVLIAILVFLPLSPTTLYVFAC
                     GIGLLWLGTVPLTNGLVGQIFGLRYMATLTAIVFFGHQIGSFIGVWLAGYLYDVTGSY
                     NGAFMTSIALGVFAALVNLPVNEKPLVERRHARAVA"
BASE COUNT          824 a         1509 c         1551 g          753 t
ORIGIN      
        1 gcgcccacgc actcgagcat ctccgcggcc aggctcttgg acgtaaagag ctgcgcgcct
       61 ggaagcagcc gcgccagcat cgcgccggcc agatcgtcgg cttcgtcatg cgcgggaagg
      121 atgcacacgt gccggcactg cgcttcggct ggctccttgt gcgggatctc ctcgacgatt
      181 tcgcgcgctg tatcgaggac aaagcgctcg cgcgcggggt cgagcgcccc cgcgtggcgg
      241 tcgccttcgg cgagcgacag cgcggggacg atgacggagt cgtacagcgc ggcaacgccg
      301 tgctcgcgcg catgttcgtg ggcgagcgcc atggcctcct cgtcgtcgag ggcgatcagc
      361 cgctggtaga ggcgcgcctc cggcgtgaga accggctcca cgccgagcag catgttgagc
      421 cagcccatct cggggatgta gcgacccatg accgcgatgc agacggtgag cggcacggcg
      481 aggagcagcc cgacaggccc ccacagccag gtccagaata gcgccgcggc gatcaccgcg
      541 atcgccgaga gcccggcgct tcggccatag agccacggct cgatcgcatt cacgaggatc
      601 agctcgagaa cgaggaaagt gccgagaacc caggtgacct cgctccagcc gtcgaaaatc
      661 gcgaacgcaa gcagcgccgg ggccgcgacc gccaaccaca cgccgacata aggaatgaac
      721 cgcagcagcg tgccaagcag cccccagagc atcgcgttgg gtacgccgat gaagtgcagc
      781 gcgatgccga acgggatgcc gaagcacgcg ttcacgagaa gcagcgtcga gagatagcgg
      841 ctgaggcggt ccgaagcctc ccccagcgcc tgggtcgtga cgttgatccg ccgcgcgccg
      901 atgaggccga tcagccgctc gcgcaggttc tcccggttga ggagcatgag gatggtgaac
      961 accaccaccg cgagcgccgt gccgatcggc ttcacgaacg gacccaccca ctcggcgagc
     1021 gcggcaaacg cgctcggcgg cgtttcggtc accggcagcg gcgccgccgc agcgggcgcg
     1081 gcctcgcttt cgagttcctt gatcgcctcg gccgctttgc cgagggcgcc atcgtccggc
     1141 gcgcgcaacg cgcgcgcctt ctcgcggatg ttctcgcggt actcgggcag cttcgccgcg
     1201 agcgaaagcg cctcgcgcgc ggcgacgtag ccgatgccgc cgatcaaccc gccggcgacc
     1261 gcgacggcga cgagtgtcga cgcgatgcga cccagccgca agtactcgag gcggcgtacc
     1321 acgggcgcga gcaggaacgc agcgaggatg gcgagcgcga gcgggatgag cacgtcgcgc
     1381 gcgaggtaca ggaccgccgc ggcgaccagc agcccggcga gcgacgaggt cttactggct
     1441 aagagcaaac agcagcagcg accgtgcggt cacctcgtac tcgtgaccga atggaaattg
     1501 cggctcttcc ttcgaatcgg ggaagttcgt gtcggcgagc agcttccagc gctcgccgcc
     1561 gttggcctgg ggaagacgga acttcaccag gtcctgccac ggattgaaga tgatgaggag
     1621 cgtcgcctcc tcgccgcgct tgtgaatgcc gctggtctgc gcacggccgt cgagcagcat
     1681 gccgaagcag cgcacgttgg catccttcca gtgctcctcg cgcatctcct cgccggtggc
     1741 attgatccag gtcacgtcct tcacgccgag ctccgcgtcg aacgtgcccg agggaaagcg
     1801 cgggcgccgc aggatcgggt agcggctgcg caggccgagc agcttctgca cgaagccgat
     1861 caggcgctgc gcccgatcgt cgtgcaccca gcgcagccag gagatctcgt tgtcctggca
     1921 gtaggcgttg ttgttgccgc gctggctgcg gccgaactcg tcgccggcga gcagcatcgg
     1981 cgtgccctgc gagagcagca gcgtggcgag catgttcttc atctggcgct tgcgcaggcc
     2041 gcggatcgct tcgtcgtccg tgggtccctc cactccgtgg ttccacgagc ggttgtgcga
     2101 atggccgtcc ttgttgccct cgcggttcgc ctcgttgtgc ttgtcgttgt acgtgacgag
     2161 gtcgtagaga gtgaaaccgt cgtgcgcggt gatgaagttg acgctcgccc acggctggcg
     2221 cccgcggcgg ttgaagatgt cgggcgagcc gcacaaccgc ggcgcgagcg cgcccggcgt
     2281 ggcttcgcct ctccagtagt cgcgcaccgt gtcgcggaac tggtcgttcc actctgccca
     2341 cccaggcggg aagcggccga gctggtagcc acccgggccg cagtcccagg gctcggcgat
     2401 cagctttacc ttggcgagca ccgggtcctg catgagcgcc ttcatgaagc cgttgtaggt
     2461 gtcgaacccg ctcggctcgc gcgcgaggat ggtcccgagg tcgaagcgga agccgtcgac
     2521 gtgcatttcc tcggcccagt agcgcaggct gtcgcacacc atctggatca ccaccgggtg
     2581 ggagagattc agcgtgttcc cggtaccggt gtcgttgatg tagtagcgcc ggttgtcggc
     2641 gatcagtcgg tagtacgacg cgttgtcgat tcccttgaag gaaagcgtcg gtccgagctc
     2701 gttgccctcg gccgtgtggt tgtagaccac gtcgatgatc acttcgagcc ccgcatcgtg
     2761 gaagcgcgcc accatctcct tgaactcctg cagcgtcttc gacgggtcgg aggcgtagcg
     2821 cgggtcgggc gcaaaaaagc cgatcgagtt gtacccccag taatttctaa gattcttctt
     2881 cagcaagaat tcatcatcga tgaaggtgtg cacgggcagc atctcgaccg aggtcacgcc
     2941 gagggacttg atgtaggcga tgacctcctt caccgccagg ccgcaatacg tgccgcgctg
     3001 gtgttccggc accctggggt gcagcttggt atagccgcgc acgtgggtct cgtagatgat
     3061 cgtccggtcc caggggaccg ggtgccattg ccgctcgcgc gcccagtcga agttcggatc
     3121 gaccaccatg cacttcggca caaaaggggc gctgtcgcgc gagtcgaacg agagatcggt
     3181 gttctcggcg cccatggtgt agccgaagat cgccgggtcc catttcagct cgcccatgtg
     3241 cgcgcgcgcg tacgggtcga gcacgagctt gttggggttg aagcgatggc cttcctcggg
     3301 agcgtacggg ccgtgcacgc ggaagccgta agcggtgccg ggaccgatgt ccggcacgta
     3361 gccatgccag atctcgtcgc ggaactcggg cagctcgatg cgctcccgtt ctttcccttc
     3421 ggaatcaaag agacaagctt cgactcgggt cgcgttggcg gagaacagcg cgaagttcac
     3481 tcccttgccg tcccaggtgg cgcctcgcgg gtagggcagg ccctccctga cccgggagag
     3541 gcgcagctcg ggtgcgttca tgtgagcgag acaatggggc gcaaggctcg tgcccggaat
     3601 gctccgttcg gcgcattcgg cgcggtcttt gctgacaccg gggcatgttg gacatggccg
     3661 gctgggaccg cttcgtaaac agccccccgg ggcgtcgttt ccaggaccgc taccgccgca
     3721 agcgccggga caagggcagc ggatggaagc gttgcgctct cgtctgcggg ggtatcgcgc
     3781 tcgccctggt gggagtcttc ttcatggccg tgccgggccc gggaattccc atcctcgcca
     3841 ttggcttggc gctcgtcgcg caagagtcgg ccgcgatggc ccggctcctt gaccgcaccg
     3901 agctccggct gcggcggctg tggaaacgcg tacggaaacg ctaactgcaa agaaccaggt
     3961 tcgcgaactc gaccagcatt tccggcttca gatacgcggc aaaagcggtc gcactcacga
     4021 acgcgaagac ggccacccat accgccttaa gccacagctc gtgcatgcct tcgctccacc
     4081 aacggcttct cattgaccgg caggttaacc agcgccgcga acactccaag cgcgatcgac
     4141 gtcatgaagg ctccattgta cgacccagtg acgtcgtaca ggtaccccgc aagccataca
     4201 ccgatgaagc tgccaatctg gtggccgaag aacacgatcg ccgtaagcgt cgccatgtag
     4261 cgcaggccga aaatctggcc cacaagcccg ttggtgagcg gcacggtccc cagccagagc
     4321 aggccgatgc cgcaggcgaa gacatagagc gttgtcggcg aaagcggaag gaacaccaga
     4381 atggcgatga gaacggcgcg taccgcgtag atggtcgaga gcaaatactt cttgctgaag
     4441 cggccaccgg cccaaccagc cccgagggtt cccacgacgt tgaacagtcc gatcagcgca
     4501 agcgcggtca tgccgtgcgt ggcgctcaac ccggcgtcgg tgacgaacgc cggcaggtgc
     4561 acggtaagca tggcgatgtg gaagccgcac acgaagtaac cccaaaacag cagatggaac
     4621 ccccgctcac gcatcgc
//
LOCUS       DASTPD010000094         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1066953, whole genome shotgun sequence.
ACCESSION   DASTPD010000094 DASTPD010000000
VERSION     DASTPD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066953"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /gene="narH"
                     /locus_tag="VFR57_03285"
     CDS             <1..1107
                     /gene="narH"
                     /locus_tag="VFR57_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEU0258429.1"
                     /translation="GPNWEEILGGEFEKRSKDYNFEAVQKEIYGEFENTFLMYLPRLC
                     EHCLNPTCVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKVYYNWESGKAE
                     KCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADAIEAAASVERPEDLYPAQLAVF
                     QDPHHPKVIEQARKDGVPEAWIAAAQASPVYKMAIDWKVAFPLHPEYRTLPMVWYVPP
                     LSPISAAANSGQLGRNGAIPDVSQLRIPLKYLANLLTAGDEAPVKLALERMLAMRAFF
                     RGLNVDKQEYPEILEQVGLSRSQIDEMYRTMAIANYEDRFVIPTTHREHAEDAYDLRG
                     SCGFSFGNGCSDGTSSPSLFGGKKRVIALKAISS"
     gene            1104..1721
                     /gene="narJ"
                     /locus_tag="VFR57_03290"
     CDS             1104..1721
                     /gene="narJ"
                     /locus_tag="VFR57_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEU0258430.1"
                     /translation="MSKALRAMAALLSYPDEALIAALPEIKAFVPKEVNALTEELACK
                     DLYSLQEQYVSLFDRSRALSLHLFEHVHGETRERGQAMVELAQMYQLHGMSVAGHELP
                     DYLPAFLEFASLVDEAKELLGQIAHILRTLGDRLAERGSPYCTVFAALLAAAGERGLT
                     KARVAPVRAAEDLLALERNWMDPEVTFGGPQPVRLYRKGELAGMT"
     gene            1718..2392
                     /gene="narI"
                     /locus_tag="VFR57_03295"
     CDS             1718..2392
                     /gene="narI"
                     /locus_tag="VFR57_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEU0258431.1"
                     /translation="MNQFLFGVYPYIALAVFLVGSLIRYDRDQYTWKSDSSQLLRRGQ
                     LRWGSNLFHLGILGIFFGHLFGLLTPLAVWHALGVTAAQKQTFAMVAGGIAGLACASG
                     LVLLIHRRLSVARVRATSRPMDFVVLYLLLAQVLLGLYSITFSLQHRDGAEMLKFVHW
                     AQGIVTFQPNAASHIAEVAWVFRAHIVLGLTLFIVFPFSRLVHIWSGFATVGYLARAY
                     QIVRSR"
     gene            complement(2403..3692)
                     /locus_tag="VFR57_03300"
     CDS             complement(2403..3692)
                     /locus_tag="VFR57_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEU0258432.1"
                     /translation="MRIPKLAPLPALLLAGAAQAADPATIDWSGVPATKLTLFYPGQS
                     TFQWLRSSGHQGAAVVGSGTPCVACHKGQEAKLGEKIVKGGPLEPTPPAGKNGSLPLS
                     VQVAYDDQNAYFRFQWKTRNNYPGDGYPYYRFDGKEWKAYGAPRLSAPAYKGEQPPIY
                     EDRLSMLIDDGSVPNFDKHGCWVTCHNGERDNPGQPSAALVAENPFYQAIKRADVRKY
                     LPVTRTDANASWDKGVPMAELEKLKAEGKFLDLIQWRAHRSNPIGMADDGYVLDWRNF
                     DSGANPFASNMDGKTKTPRFMFKGADKAFSDANPPKNGGVLIAGQNAVPFDPNAGWKA
                     GDLLPQYYLSAEGAKGSAADNKYAKGVWKDGTWTLVWARPLNLRNPDDKALQEGKSYT
                     FGFAVHDDNITTRGHHVSLPVSVGFGAKGDIQATKLK"
     gene            3794..>3992
                     /locus_tag="VFR57_03305"
     CDS             3794..>3992
                     /locus_tag="VFR57_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258433.1"
                     /translation="MKMRTLFVTATLAAVLALPASAQQMKTVGGIVVNIGVVNAITAE
                     HADAQHGVHKGGHGAGMEHIVV"
BASE COUNT          667 a         1277 c         1290 g          758 t
ORIGIN      
        1 gggccgaact gggaggagat cctgggcggg gaattcgaga agcgctccaa ggattacaac
       61 ttcgaggccg tccagaagga aatctacggc gagttcgaga acacgttcct gatgtacctg
      121 cctcgcctgt gcgagcactg cctcaacccc acctgcgtcg cgagctgccc ctccgggtcg
      181 atctacaagc gcgaggagga cggcatcgtc ctcatcgacc aggacaagtg ccggggctgg
      241 cgcatgtgcg tgtccggctg cccgtacaag aaggtttatt acaactggga gagcggcaag
      301 gccgagaagt gcatcttctg ctacccgcgc atcgaggccg ggcagccgac ggtgtgctcg
      361 gaaacctgcg tcgggcgcat ccgctacctc ggcgtgctgc tctacgacgc cgatgcgatc
      421 gaggccgcgg cgagcgtcga gcggcccgag gatctctacc cggcgcagct cgcggtgttc
      481 caggatccgc accacccgaa ggtcatcgag caggcgcgca aggacggcgt gcccgaggcg
      541 tggatcgccg cggcgcaggc ttcgcccgtc tacaagatgg cgatcgactg gaaagttgcg
      601 ttcccgctgc acccggaata ccgcacgctg cccatggtgt ggtacgtgcc gccgctttcg
      661 ccgattagcg ccgcggccaa cagcgggcag cttgggcgca acggcgcgat tccggacgtt
      721 tcgcagctgc gcataccgct caaatacctc gccaacctgc ttacggcggg cgacgaagcg
      781 cccgtgaagc tcgccctcga gcgcatgctc gcgatgcgcg ccttcttcag gggcttgaat
      841 gtcgataaac aggaataccc ggagatcctg gagcaggtgg gcctctcccg ctcgcaaatc
      901 gacgagatgt accgcaccat ggcgatcgcg aactacgagg accgcttcgt catcccgacg
      961 acgcaccgcg agcacgccga ggatgcgtac gacctgcgcg gctcgtgcgg cttcagcttc
     1021 ggcaacggct gctccgacgg caccagcagc ccgagcctgt tcggcggcaa aaagcgcgtc
     1081 atcgcgctca aggcgatctc gtcatgagca aagcgcttcg tgcgatggcg gcattgttgt
     1141 cgtatccgga cgaggcgctg atcgcggcgc ttccggaaat caaggccttc gttcccaagg
     1201 aggtcaacgc tctcacggaa gaattggcgt gcaaggatct ctacagcctg caagagcagt
     1261 acgtgtcgct tttcgaccgc tcgcgcgcgt tgtcgctgca tctcttcgag cacgtgcacg
     1321 gcgagacccg ggagcgcggg caagcgatgg tcgagctggc gcagatgtat caactgcacg
     1381 gcatgagcgt cgccgggcat gagctgccgg attacctgcc ggctttcctg gagttcgcct
     1441 ctctggtgga tgaagcgaag gagctccttg gacaaatcgc gcatatcctg cgcacgctcg
     1501 gcgatcggct ggccgagcgg ggcagcccct attgcaccgt gttcgccgcg ctgctcgcgg
     1561 ccgcggggga aagaggcctc acgaaggcgc gggtcgcgcc agtgcgcgcg gccgaggacc
     1621 tgctcgcgct cgagaggaac tggatggatc ccgaggtcac gttcggcggg ccgcaaccgg
     1681 tgcgcctgta tcgcaaaggt gaactggcag gaatgacatg aaccaatttc tcttcggcgt
     1741 gtatccctac attgccctcg ccgtgttcct ggtcggcagc ctcatccgct acgaccgcga
     1801 ccagtacacc tggaagagcg attcaagcca gctcctgcgc cgcggccagc tgcgctgggg
     1861 gtcaaatctt ttccacctcg gcatcctcgg cattttcttc ggccatctct tcggcctgct
     1921 gacgccgctc gcggtgtggc acgcgcttgg cgtcaccgcg gcgcagaagc agaccttcgc
     1981 catggtcgcc ggcggcatcg ccggcctcgc ctgcgcgagc gggctcgtgc tcctcatcca
     2041 tcgccgcctg agcgtagcgc gcgtgcgcgc cacctcgcgg ccgatggatt tcgtcgtgct
     2101 ctatctgctg ctcgcgcagg tgctgctcgg cctgtacagc atcacgttct cgctccagca
     2161 ccgcgacggt gcggaaatgc tcaagttcgt gcactgggcg caggggatcg tcacgttcca
     2221 gccgaacgcc gccagccaca tcgcggaggt cgcctgggtg tttcgcgccc atatcgtcct
     2281 cgggctgacg ctgttcatcg tgttcccgtt ctcccggctg gtgcacatct ggagcgggtt
     2341 cgcgacggtc gggtatctcg cgcgcgccta ccagatcgtg cgctcccgct gatcccgaag
     2401 gcttacttca gcttcgtagc ctggatgtcg cccttggcgc cgaaccccac gctgacggga
     2461 agggaaacgt gatggccgcg cgtcgtgatg ttgtcatcgt gcacggcgaa gccgaaggtg
     2521 taactctttc cttcctgtag cgccttatca tcgggattgc gcaggttgag cggtcgcgcc
     2581 cacacgagcg tccaggtacc gtctttccac acgcccttcg cgtacttgtt gtcggccgcc
     2641 gaccctttcg ctccctcggc gctcaagtag tactgcggca gcaggtcgcc cgctttccaa
     2701 cccgcattcg ggtcgaaggg cacggcgttc tggccggcaa tcagcactcc gccatttttc
     2761 ggcgggttcg cgtcgctgaa agccttgtcg gcacctttga acatgaagcg cggcgtctta
     2821 gttttgccgt ccatgttgga ggcaaacggg ttcgcgccgg agtcgaaatt gcgccagtcg
     2881 agcacgtagc cgtcgtcggc catgccgatg ggattggagc ggtgcgcgcg ccactggatg
     2941 agatcaagga acttgccttc ggctttgagc ttctcgagct cggccatcgg cacgcccttg
     3001 tcccacgagg cgttcgcatc ggtgcgcgtg accggcaggt acttgcgcac gtcggccctc
     3061 ttgatcgcct ggtagaacgg gttttcggcg accagcgcgg cgctcggctg cccggggttg
     3121 tcacgctcgc cgttgtggca ggtcacccag cagccgtgct tgtcgaagtt cggcaccgag
     3181 ccgtcgtcga tgagcatgga gaggcgatcc tcgtagatcg gcggctgctc acccttgtag
     3241 gccggcgccg aaagccgcgg cgcgccgtaa gctttccact ccttgccgtc gaagcgatag
     3301 tacggataac cgtcgcccgg atagttgttc ctggtcttcc actggaagcg gaaataagcg
     3361 ttctggtcgt catacgcgac ctgcacgctt aacggcagcg agccattctt tccggcgggc
     3421 ggcgtgggtt cgagcggccc acctttcaca attttttcgc cgagcttggc ttcctgccct
     3481 ttgtgacagg caacgcaagg cgtgccagag ccgacgactg cggcaccctg atgtccggag
     3541 ctgcgcagcc actgaaacgt cgactgcccg ggatagaaca gtgtgagctt cgtggcgggc
     3601 acgccgctcc agtcgatggt ggcgggatcc gcggcttgtg cggcgccggc aagcagcagg
     3661 gcggggagtg gtgcgagttt cgggatcctc atcgggcctc cgagcgtaat tccggatcgt
     3721 aggcgccgcg tttgatctgc gtcaatcgcg agccaaatgc ccatgctcca ctggcagcag
     3781 aaggaggcgg atcatgaaga tgcggacatt gtttgtaaca gcgacgctcg ccgcagtatt
     3841 ggctttgccg gcctcggcgc agcagatgaa gacggttggc ggcatcgtgg tcaacatcgg
     3901 cgttgtcaat gccatcacag ccgagcacgc ggatgcgcag cacggcgtgc acaaaggcgg
     3961 ccatggcgcc ggcatggagc acatcgtggt cg
//
LOCUS       DASTPD010000095         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_659737, whole genome shotgun sequence.
ACCESSION   DASTPD010000095 DASTPD010000000
VERSION     DASTPD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659737"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFR57_03310"
     CDS             complement(<1..714)
                     /locus_tag="VFR57_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEU0258434.1"
                     /translation="MGGKGAVFVPGGVSFEGGWDTCYRTNLRSRIASRVLWKVQDFAY
                     RNEDDVYKAVKALEWPRYFDVERTLRVNVTAQKSPLRSLEFATLRVKDAVCDRFRETL
                     GRRPHVERAAPDVRIHLFLARDTGTLYLDTSGEPLFKRGWRAGQGEAPLRENLAAGIV
                     MLTRWRSDEPLLDPMCGGGTLLVEAAALARGRAPGAKRGFGFQNLKSFKAPLWETVKR
                     ENMADSSNPVLYGSDNDPEA"
     gene            complement(776..1192)
                     /locus_tag="VFR57_03315"
     CDS             complement(776..1192)
                     /locus_tag="VFR57_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU0258435.1"
                     /translation="MLWAKSFHVVFMVTWFAGLFYLPRLFVYHVLAQDRPSIERFKLM
                     ERKLFWGIMTPGAALTIAFGVWLWLGWFRGSSSWLHAKIALVAILVGYHLWCWRLMRD
                     FAAERNTKSHVWFRWFNEFPVLILVATVLLVVFKPF"
     gene            complement(1194..1943)
                     /locus_tag="VFR57_03320"
     CDS             complement(1194..1943)
                     /locus_tag="VFR57_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258436.1"
                     /translation="MRWISIWRRNMLVWRKLAAAAMLSNLADPLIMLFGLGYGLGALL
                     PSVQGMSYIAFFAAGQLCTATMFTASFESMFSSFARMHGQKTWDAILHAPLTIDDIVA
                     GEIVWAASKAWLTGSTILGVAVVFGLASSPWVLLALPAAFLVGLAFAAVGLIMCVLAR
                     GWDFFSYYMTLVMTPMMMISGVFFPAEQLPAPLLAVAKALPLYHGVQIVRPLIAGGPP
                     PALWLHVAVLLAYAAAGYAAAIYFARRRFSS"
     gene            complement(1946..2848)
                     /locus_tag="VFR57_03325"
     CDS             complement(1946..2848)
                     /locus_tag="VFR57_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0258437.1"
                     /translation="MSALSARGLRKSYGALEVVSGVDLAVAPGECFGLLGPNGAGKTT
                     TLKLCLGLIEPDSGHIELLGEPVPRRAREARARVGVVPQFDNLDPDFTVEENLVVYAR
                     YFGIDSRDISARIPALLDFAGLAGRRDAKIMALSGGMKRRLTLARALVNDPQLVFMDE
                     PTTGLDPQARHLIWERLRRLTQEGKTLVLTTHFMEEAERLCHRLAIMDRGRIIAEGSP
                     RTLISEHIEPQVLEVHGPGHEAWMERAKSLAPRVERVGDTVYCYGEDVEPLLKTLFGD
                     LSYLHRPASLEDVFLKLTGRDLRD"
     gene            complement(2845..3438)
                     /locus_tag="VFR57_03330"
     CDS             complement(2845..3438)
                     /locus_tag="VFR57_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0258438.1"
                     /translation="MRPSTRREFIAGPAGRIECAVDSPEGAARGVALIAHPHPLFGGT
                     LDNKVVQTLARAFVDLGYEAWRPNFRGVGATEGLHDDGRGELEDLRTIVRRLAPAKLV
                     LAGFSFGASLQARLAQEVELEWLVLVGIGITRLEAPAVPEGTLVIHGERDDTVPLAAV
                     LDWARPQDLPVVVVPGADHFFHRKLQVIRRIVQANAR"
     gene            complement(3438..3746)
                     /locus_tag="VFR57_03335"
     CDS             complement(3438..3746)
                     /locus_tag="VFR57_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU0258439.1"
                     /translation="MSHYAKHVFFCCNQRDGERTCCNDKGASRMRDYAKGRIKELSLA
                     GPGKIRVNQAGCLDRCEEGPCIVVYPEAVWYTYVDRADIDEIIEEHLQKGRIVKRLRM
                     "
     gene            complement(3759..>4072)
                     /locus_tag="VFR57_03340"
     CDS             complement(3759..>4072)
                     /locus_tag="VFR57_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258440.1"
                     /translation="ENVFTWLTHGAQLGLVIGVAVALAAVALPRTVRLVVAAVLLMAA
                     TVLVNLAPPNPYFALSLKVWHQGHFLNFNGLTRLVSAAWPFIALGYLIYLASRRRDAL
                     G"
BASE COUNT          779 a         1331 c         1337 g          625 t
ORIGIN      
        1 tgcttcgggg tcgttatcac tgccatacag gacgggattg gagctgtcgg ccatgttttc
       61 ccgtttcacg gtctcccaaa gtggagcctt aaagctcttc aagttctgaa atccaaaacc
      121 gcgctttgcg ccaggcgcgc ggccgcgcgc caacgcggct gcctcgacga ggagagtgcc
      181 gccgccgcac attgggtcga ggagcggctc gtccgaccgc cagcgggtca gcatgacgat
      241 gccggcggcc aggttctcgc gcagcggcgc ctcgccttgc cccgcgcgcc agccgcgctt
      301 gaagagcggc tcgccggaag tatcgaggta aagcgtgccc gtgtctctcg ccaggaacag
      361 atggatccgc acgtcgggcg ctgcgcgctc gacgtgcggg cgacggccca gcgtctcgcg
      421 gaaacggtcg cacaccgcgt ccttcacacg cagcgtggcg aactcaaggc tgcgaagggg
      481 actcttctgc gcggtcacgt tgacgcgcag cgtgcgctcg acgtcgaagt agcgcggcca
      541 ttccagcgct ttcaccgctt tgtagacatc atcctcgttg cggtaggcaa agtcctggac
      601 cttccacagc acccgcgagg cgatgcgcga ccgaaggttc gtccggtagc acgtgtccca
      661 gccgccttcg aaggagacgc cgccgggtac gaagacggca cctttccctc ccaatgagga
      721 aatttcctgc tgcagaagca gttcgagccc gcgcggcgcg gtggcgaaga aagcgctaga
      781 agggcttgaa gacaacgagc agcaccgtgg cgacgaggat gagcaccggg aattcgttga
      841 accagcggaa ccagacgtga ctcttggtat tccgctcggc agcgaagtcg cgcatcaggc
      901 gccaacacca gaggtggtag cccacgagaa tcgccacgag cgcgatcttc gcgtgcagcc
      961 agctcgagct gccgcgaaac cagccgagcc agagccacac gccgaaggcg atggtgagcg
     1021 ccgcgcccgg ggtcatgatg ccccagaaga gcttccgctc cattagcttg aagcgctcga
     1081 tggagggccg gtcctgcgcg agtacgtggt aaacgaacag ccgcggcaga tagaaaagcc
     1141 ccgcgaacca cgtcaccatg aaaacgacgt gaaaggactt ggcccagagc atgtcaagac
     1201 gagaaacggc ggcgggcgaa gtagattgcc gccgcatagc cggccgcagc gtaggcgagc
     1261 aacaccgcca cgtgcagcca cagggcaggc ggcggaccac cggcgataag cgggcgcaca
     1321 atctgcacgc cgtggtagag cggcagcgcc ttggccaccg cgaggagcgg cgccggcagc
     1381 tgctcggcgg ggaagaacac tcccgagatc atcatcatcg gcgtcatgac gagggtcatg
     1441 taataactga aaaagtccca gccgcgcgcg agcacgcaca tgatgaggcc caccgcggcg
     1501 aacgccagtc cgacgaggaa cgccgccggc agcgcgagca gtacccacgg cgagctcgcc
     1561 agcccgaaca ccaccgcgac ccccaggatg gtggaacccg tgagccacgc cttgctcgcg
     1621 gcccacacga tctcgcccgc gacgatgtcg tcgatcgtca gcggcgcatg cagaatggcg
     1681 tcccaggtct tctggccgtg catgcgcgcg aaactggaga acatggattc gaacgacgcc
     1741 gtgaacatgg tcgccgtaca gagctgtccg gcggcgaaga atgcgatata cgacatgccc
     1801 tgcaccgaag gcagcagcgc ccccaggccg tagcccagcc cgaacagcat gatcagggga
     1861 tcggcgaggt tgctcagcat ggccgccgca gcgagcttgc gccagaccag catgtttctg
     1921 cgccaaatcg agatccagcg cattctcaat ccctgagatc gcggcccgtt agcttgagaa
     1981 aaacgtcttc caggctcgcg gggcggtgga ggtagctcag atcgccgaaa agcgtcttga
     2041 gcaacggctc gacgtcctcg ccgtagcagt aaacggtgtc gcccacgcgc tcgacgcgcg
     2101 gcgccaggct cttcgcgcgt tccatccacg cctcgtggcc aggcccgtgc acctcgagca
     2161 cctgcggctc gatgtgctcg gagatcagcg tgcgcggcga gccttcggcg atgatgcggc
     2221 cgcgatccat gatggcgagc cggtggcaga gcctctccgc ttcctccatg aaatgcgtgg
     2281 tcagcactag cgttttgcct tcctgcgtca gccgccgcaa gcgctcccag ataaggtggc
     2341 gcgcctgggg atcgaggccg gtggtcggct cgtccatgaa cacaagctgc gggtcgttga
     2401 cgagcgctcg ggctagcgtc aggcggcgct tcatgccgcc cgacagagcc atgatcttcg
     2461 cgtcgcggcg acccgccagc ccggcgaagt cgaggagagc gggaatccgg gccgaaatat
     2521 ccctggagtc gatcccgaag tagcgtgcat agacgaccag gttctcctcg acggtgaaat
     2581 ccgggtcgag gttgtcgaac tgcggcacga ctcccactcg cgcgcgtgct tctcgggccc
     2641 gcctcggcac gggctcgcca aggagctcga tgtggccaga gtcgggctcg atcaggccga
     2701 ggcagagctt gagcgtggtg gtttttccgg cgccgttagg gccgaggagg ccgaagcatt
     2761 ctcccggcgc cactgcgaga tcgacgcccg agacgacttc aagcgcgcca taagacttgc
     2821 gcagcccccg cgccgagagg gcgctcatct cgcgttggct tgcacgatcc gtcggatgac
     2881 ctgcagcttg cgatggaaga aatggtccgc gccgggcacc acgactacgg gcaagtcctg
     2941 cggccgcgcc caatcgagca ccgcggcgag gggcacggta tcgtcgcgct cgccgtggat
     3001 cacgagcgtg ccttccggca cggccggcgc ctcgaggcgc gtgatgccga tgccgacgag
     3061 taccagccac tcgagctcaa cttcctgcgc gaggcgagcc tgtagcgacg ccccgaagga
     3121 gaacccggcc agaacgagct tcgcgggcgc caggcgcctc acgatggttc tcaagtcctc
     3181 gagctcgcct cgcccgtcat cgtgcaagcc ctcggtggcg ccgacgccgc ggaaattcgg
     3241 ccgccaggcc tcgtagccca ggtcgacgaa ggcgcgcgca agcgtctgca cgaccttgtt
     3301 gtcaagcgtg ccgccgaaga gcggatgcgg gtgcgcgatc agcgcgacgc ctcgcgccgc
     3361 gccttcggga ctgtcgacag cgcattcgat acgccctgcc gggccggcga tgaactcgcg
     3421 acgggtactc ggccgcatta catcctgagg cgttttacga tgcgcccttt ctgcagatgc
     3481 tcctcgatga tctcgtcgat gtcggcccgg tcgacgtagg tgtaccagac cgcttcgggg
     3541 tacacgacga tgcacgggcc ttcctcacag cgatccaggc accctgcctg attgacgcga
     3601 atcttgcccg ggccggcgag gctgagctcc ttgatgcggc ccttggcata gtcccgcatc
     3661 cgcgaggcgc ccttgtcgtt gcagcaggtg cgttcaccgt cgcgctgatt gcagcagaaa
     3721 aagacatgct tggcgtaatg ggacatgagc cggccattct agccgagcgc gtcgcgccgc
     3781 ctcgacgcga ggtagatgag atagccgagc gcgatgaagg gccaggcggc gctcaccagc
     3841 cgcgttagcc cattgaagtt gagaaagtga ccctggtgcc atactttcaa cgaaagggcg
     3901 aagtagggat tcggtggcgc gagattcacc aacacggtgg cggccatcag aagaaccgcg
     3961 gccacgacca accggacggt ccggggcagc gccacggcgg cgagcgcgac cgcgacgccg
     4021 atgacgaggc cgagctgcgc tccgtgcgta agccatgtga aaacgttttc gg
//
LOCUS       DASTPD010000096         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1271940, whole genome shotgun sequence.
ACCESSION   DASTPD010000096 DASTPD010000000
VERSION     DASTPD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271940"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..306)
                     /locus_tag="VFR57_03345"
     CDS             complement(85..306)
                     /locus_tag="VFR57_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258441.1"
                     /translation="MSTSKPASAAVTAAYLMICAALGYFFGDLADAGWAILIGALAPY
                     ALFYAVTLFGLALGAEPSRMQRRRRRFRR"
     gene            487..636
                     /locus_tag="VFR57_03350"
     CDS             487..636
                     /locus_tag="VFR57_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258442.1"
                     /translation="MKQRITGLKRRQIAAAFRRMARGVEEKAAYGRTIGFYRASGQAW
                     VERLG"
     gene            complement(693..920)
                     /locus_tag="VFR57_03355"
     CDS             complement(693..920)
                     /locus_tag="VFR57_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258443.1"
                     /translation="MNWSTIESKWDDYRSDAQKRWGKLTDEQMRGTMGSRNQLSARLQ
                     QAYAVTEADADRWISEWQHKQKEKPAGAAKS"
     gene            1428..1583
                     /locus_tag="VFR57_03360"
     CDS             1428..1583
                     /locus_tag="VFR57_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258444.1"
                     /translation="MDATTLTNEVLPELEAELETWDWARTAGVSADELRQALRAKLSD
                     PALRKAA"
     gene            complement(1585..1746)
                     /locus_tag="VFR57_03365"
     CDS             complement(1585..1746)
                     /locus_tag="VFR57_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258445.1"
                     /translation="MDQPQKATQRTQYQGPERRRSTTPYTGKERRRLDWPFPARPRDP
                     QPGEGNKRP"
     gene            complement(1772..2134)
                     /locus_tag="VFR57_03370"
     CDS             complement(1772..2134)
                     /locus_tag="VFR57_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEU0258446.1"
                     /translation="MEINRLLASAERALKDAAVAALSSDTRLRTAYGALVQAALAALF
                     AKGYRPTTSEPGHHQLVIQVLSLTMKVPGERIQVLDAYRKARNQSEYRGIPVSDAIAN
                     ECLEDARRLVGEVRKSGL"
     gene            complement(2136..2756)
                     /locus_tag="VFR57_03375"
     CDS             complement(2136..2756)
                     /locus_tag="VFR57_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0258447.1"
                     /translation="MDLGSLLFGSYRKSVLALLLLHPEQALHVRELARQLDKPPGTLL
                     RELNALADAGVLVRTPVGNQVQFQANAACPVYEELRGILKKTVGVADVLRESLVSIAP
                     KISAAFVYGSVASDEERPGSDLDLMIVGDARFADVVGALASAQALLRREVNPSVYSAL
                     EFKKKLTAGEPFLRRVLAGQKIFIIGGEDELGKLGAHRKAQGARRS"
     gene            complement(2838..3146)
                     /locus_tag="VFR57_03380"
     CDS             complement(2838..3146)
                     /locus_tag="VFR57_03380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0258448.1"
                     /translation="MIHVIAVITAKSGKRDEVLKHFRANVPAVRAENGCIEYGAAIDA
                     EAALPIQTKYGPHTFVVVEKWESMDALKAHAAAPHMKEYGANTKDLLASRVIHILSPT
                     "
     gene            complement(3185..3511)
                     /locus_tag="VFR57_03385"
     CDS             complement(3185..3511)
                     /locus_tag="VFR57_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258449.1"
                     /translation="MPRKHRHNVYVIELDPAIYNSRRFRRSNPEHDITKPCVYVGCTG
                     LTPEDRFAKHKAGIRANRYVQRFGLRLLPKLYAYANPMPYDAARDMEVELAIALRAQG
                     YAVWQG"
     gene            complement(3522..>3938)
                     /locus_tag="VFR57_03390"
     CDS             complement(3522..>3938)
                     /locus_tag="VFR57_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEU0258450.1"
                     /translation="CMALDPDLLAAYQDSDYVVYEKPELVLKIGVPSERLDALLEKER
                     APCAAFVTAANPRSKPRTPEENAAALAGLDQLVAASGYPSRRGEGRESNGSWVEPSRL
                     VIGITRENAEALGRLFGQNAIVFIEKGRAPELVVLR"
BASE COUNT          679 a         1241 c         1303 g          715 t
ORIGIN      
        1 gtggacgcgc tacgggacgt cgggatcaat cacttcgaca tgccggcaag cccgccgcgg
       61 gtgtgggcgg cgatcagaaa agcgtcagcg tcggaatcgc cgcctgcggc gctgcatccg
      121 cgacggctcg gcgccgaggg ccaggccgaa tagcgtcact gcgtagaaca gcgcatacgg
      181 cgcgagggcg ccgatgagga tcgcccaccc ggcatcggcc aggtcgccga agaagtagcc
      241 gagcgcggcg caaatcatca gatacgccgc cgttaccgcc gcgctggccg gcttcgaagt
      301 gctcatgcgg gacatcatgc agagcgtgga cggaagggcg ttattccggc tgcgccaagc
      361 gtttgcccca atacgccagt cgcacgggcc gttccgcaga aacacacggg caacaaaacc
      421 gcccaccaag gaaatctgcg ggttaccggc gccgcttaag gtgaggcttc ctcataggag
      481 gcaaccatga agcagcgcat caccggtctc aagcgcaggc agatcgcggc ggcgttccgc
      541 cgcatggcgc gcggcgtcga agaaaaggcc gcgtatggaa gaacgatcgg cttttaccgc
      601 gcgagcggcc aagcctgggt cgagcggctc ggataagtcg actgcgaaac cacaggagcg
      661 cgggcctttg cacccgcgct cccgcgcctc tcctagcttt tcgcggcgcc cgcgggcttc
      721 tccttctgct tgtgctgcca ttcgctgatc cagcgatcgg cgtcggcctc ggtcacggca
      781 taggcctgct gcaggcgggc ggacagctgg ttgcggctgc ccatcgtgcc cctcatctgt
      841 tcgtcggtga gcttgcccca ccgcttctgg gcgtcgctcc tatagtcgtc ccacttgctc
      901 tcgatcgtgc tccagttcat gtgcttcctt cgcgtgcaaa acacttcttc ccgcacgccg
      961 ggcggcgtcc agggcgctgt gctctcggga gaggcattga cggatcgccg ctcgcgcggc
     1021 gttgacgagg ctactgccgg aggagaccca ccatacgagc cgccctccag ggggtcaagg
     1081 catttcgggc gacaagcgga acgagattgt cccgttcctc tacagggatt cacgcagccg
     1141 gcggccgatt tctggtccgg acctcaaacg gcccatagaa acaaggcggc aaacaaaatc
     1201 gctcgggaaa gaaatcggcg cgctacccgc cccgcctagc cttgagcctc cctaaaggag
     1261 gcagccatga aacagcgcat caccggcagc gtatggaaga acgatcggct tctactgcgc
     1321 gagcggcagg cctgggccga gcggcttggg caaatccgac tcgtaataat ccgtatgaac
     1381 tagggcccac ggcacgcgtt gtgcggcgtt cagggataga tagcactatg gacgctacga
     1441 ctcttacgaa cgaagtgttg cccgagctcg aagccgagct cgagacttgg gactgggcga
     1501 ggaccgccgg cgtgtcggcc gacgagctcc gccaggccct gcgtgcaaag ctttccgatc
     1561 ccgcgctgcg caaagccgcg tagcttaggg gcgcttattc ccctcgcctg gctgcgggtc
     1621 gcgcggccgg gcggggaacg gccaatcgag ccgccgccgc tctttgccgg tgtacggcgt
     1681 ggtcgagcgc cggcgttcag gtccctggta ctgcgtgcgt tgggttgctt tctggggctg
     1741 gtccatctct agcgtgtagc aagaatcggg gtcagagccc ggatttcctg acctcgccaa
     1801 caagtcggcg cgcatcttct aagcattcat tggcgatagc gtccgatacg gggataccac
     1861 ggtattcgct ctggttgcgc gccttgcgat aggcatcgag aacctgaatc cgctcgccgg
     1921 gcaccttcat cgttagagat aggacctgga tgacgagctg gtgatgaccg ggctcgctgg
     1981 tggtcggccg atagcctttt gcgaacaacg ctgcgagcgc cgcctggaca agggcgccgt
     2041 aggcggtcct aagccgggta tcgctgctca gcgcggccac cgctgcatcc ttaagcgcgc
     2101 gctcggccga ggcgagcagc cgattgatct ccaccctatg agcggcgtgc gccttgagct
     2161 ttccgatgcg cgccaagttt tccaagctca tcttcgccgc cgatgatgaa gatcttctgc
     2221 cccgcaagga ctcgccgcag gaatggctct cccgcggtga gcttcttctt gaactccaac
     2281 gcggaataga cgcttgggtt gacctcacgc cggagcaacg cttgcgccga cgcgagcgcg
     2341 ccgaccacgt cggcgaagcg cgcgtcaccc acgatcatga ggtcgaggtc actgccgggg
     2401 cgctcctcgt cactcgcgac tgagccgtag acgaaggcgg ccgagatctt gggcgcgatg
     2461 ctgaccagcg attcgcgcag cacatcggca acgcccacgg tcttcttcag gatgccgcgc
     2521 aattcctcgt agacaggaca tgcggcgttc gcctggaatt gcacctggtt accaaccggt
     2581 gtgcgcacca atacgccggc gtccgcgagc gcgttcagtt cacgtagcag cgttccggga
     2641 ggcttatcga gctgtcgcgc gagttcgcgc acgtgcagcg cctgctccgg atgcaggagg
     2701 agcagagcaa gcacgctctt acggtagctg ccgaaaagaa gcgagcccag gtccatagcc
     2761 gttgccgaaa aggcgtcaac tgtagcctta tttgcaacgg tccgtcaaac gatctgcaaa
     2821 tcggctccga ccccgaatta cgtcggcgaa aggatgtgga tcacgcggct cgcgaggagg
     2881 tccttggtgt tcgcgccgta ctccttcata tgcggtgccg cggcgtgcgc tttgagcgcg
     2941 tccatgcttt cccacttctc gaccaccacg aaggtgtgcg ggccgtattt ggtttgaatc
     3001 ggcagcgccg cttcggcatc gatcgcggct ccgtattcga tgcagccatt ctcggcgcgc
     3061 acggcgggga cgttggcgcg gaagtgcttg agcacttcgt cgcgcttgcc ggatttggcg
     3121 gtgatgacgg cgatgacatg gatcattcat tcctccagta gagaattcgc accctgaccc
     3181 cgaattagcc ttgccagacg gcgtagccct gcgcgcgcag ggcgatggcg agctcgactt
     3241 ccatgtcgcg cgcggcgtcg tagggcatcg ggttcgcgta agcgtagagc ttgggcagaa
     3301 ggcgcaggcc gaagcgctgc acgtagcggt tcgcgcggat cccggccttg tgcttggcga
     3361 agcggtcctc cggcgtgagg ccggtgcagc ccacgtagac gcagggcttg gtgatgtcgt
     3421 gctcggggtt ggagcggcga aagcgccggc tgttgtagat cgccggatcg agctcgatca
     3481 cgtacacgtt gtggcgatgc ttgcgcggca cgcgcgctac tttagcggag cacgacgagc
     3541 tcgggcgcgc ggcctttctc gatgaagacg atcgcgttct ggccgaagag gcggccgagc
     3601 gcttcggcgt tctcgcgcgt gatgccgatg acgaggcggc tcggctcgac ccagctgccg
     3661 ttcgattcgc gtccttcgcc tctgcgcgac gggtagccgg aggcggcgac gagctggtcc
     3721 aggccggcga gcgcggccgc gttctcttcc ggcgtgcgcg gcttgctgcg cgggttggcc
     3781 gcggtgacga acgcggcgca cggcgcgcgc tctttttcga gcagcgcatc gagccgctcg
     3841 ctcggcacgc cgatcttgag gacgagctcg ggcttctcgt agacgacgta gtcagagtcc
     3901 tggtaggcgg cgagcaggtc gggatcgaga gccataca
//
LOCUS       DASTPD010000097         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1068475, whole genome shotgun sequence.
ACCESSION   DASTPD010000097 DASTPD010000000
VERSION     DASTPD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068475"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR57_03395"
     CDS             complement(<1..206)
                     /locus_tag="VFR57_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0258451.1"
                     /translation="MRSFSRLFWSIITCSLALVAGCESSPQFKSTDITGAPYGRTLEL
                     VDHTGKARRLEDWRGKAVVLFFGFT"
     gene            complement(163..1050)
                     /gene="cyoE"
                     /locus_tag="VFR57_03400"
     CDS             complement(163..1050)
                     /gene="cyoE"
                     /locus_tag="VFR57_03400"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU0258452.1"
                     /translation="MNTHVLPRSAAQRLRAFYSLTKPRVVTLIVFTAVIGMFLATPGM
                     VPLRTLLAATAGIWLVAGAAAAVNCLVEQRIDAVMSRTRWRPLPQGELTAAQTLAFAG
                     ALGGLGLWLLHHHVNALTMWLTLATFVGYAIIYTVLLKPATPQNIVIGGASGAMPPVL
                     GWAAVTGEVTIEPLLLFLIIFAWTPPHFWSLALYRTEDYLRAGVPMLPVTHGKEYTRL
                     QVLLYTLILFAVSLLPYVIRMSGVVYLAAAVALGALFVAYAFRIYVAHSDAVARKTFR
                     YSIAYLALLFAALLVDHYL"
     gene            complement(1082..1591)
                     /locus_tag="VFR57_03405"
     CDS             complement(1082..1591)
                     /locus_tag="VFR57_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEU0258453.1"
                     /translation="MNLFRNKLLWVALVCAAPLVLGTGAYLLRWSPGAPSNYGELLTP
                     TLVAGLEALRGKWVLVMFDASACDARCEQKLYYMRQVRRAQGKDMSRVERLWVITDGA
                     RPREEILPGIEGTRLAVLSEKEFPGKTVDHIYLVDPLGNLMMRFPRDPDPSRMIKDLQ
                     RLLRYSRFG"
     gene            complement(1588..2217)
                     /locus_tag="VFR57_03410"
     CDS             complement(1588..2217)
                     /locus_tag="VFR57_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU0258454.1"
                     /translation="MGAGYSFRPRLWALALAAAACAAAIALGNWQRGRADEKRAAAAR
                     LERVAVEGEFVPKHTIYLDNKTRRGRPGYEVVTPLKLRGSPSYVLVNRGWLAAGTTRD
                     ALPEVLTPAGEVRIEGIALERLPRILTIEKNSGKLRQTLDVAGYAAETGLALAPRVIE
                     QHSFLPDGLVRDWPPHDTGIEKHQAYALQWYSLAALAVVLVVVLSFRKR"
     gene            2216..2413
                     /locus_tag="VFR57_03415"
     CDS             2216..2413
                     /locus_tag="VFR57_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEU0258455.1"
                     /translation="MRYVVIAIFLAIVASLGSAAVFMLRGRGDSRRMARALAWRVGLS
                     VALFVLLIAGYALGLIRPGNL"
     gene            complement(2408..>3137)
                     /locus_tag="VFR57_03420"
     CDS             complement(2408..>3137)
                     /locus_tag="VFR57_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU0258456.1"
                     /translation="WVSIAVGFMLLLYMMVRWFGDVIRESEGGKYGSWEDVSFRWGMS
                     FFIFSEVMFFAAFFGALYWARIHSVPDLASLEHKALMWPGFSGQWPSAGPNFIEKFQI
                     MGAWGLPALNTALLLSSGVTVTFAHWALIRNDRPKLLLWLALTILLGVTFLGVQAYEY
                     GHAYSDLNLKLSTGVYGSTFFMLTGFHGFHVTIGAIMLTVIWFRCYFGHFKPDHHFAF
                     EGVAWYWHFVDVVWLGLFIFVYWL"
BASE COUNT          610 a         1012 c         1048 g          467 t
ORIGIN      
        1 gtgaagccga aaaagagcac cacggccttg ccgcgccagt cctcgaggcg ccgggccttc
       61 ccggtgtgat cgacgagctc gagcgtgcgg ccatacggcg ccccggtgat gtcggtgctc
      121 ttgaattgcg ggctggattc gcagcccgcg acaagcgcca ggctacaggt aatgatcgac
      181 cagaagagcc gcgaaaagga gcgcaaggta ggcgatcgaa tagcggaagg tcttccgcgc
      241 caccgcgtcg ctgtgggcga cgtagattct gaaagcataa gcaacgaaca gcgcgccgag
      301 cgccacggcc gccgcgaggt atacgacgcc gctcatgcgg atgacgtacg gaagaaggct
      361 gacggcgaaa agaatcagcg tatagagcag gacctgcagc cgggtgtact ccttgccgtg
      421 cgtgaccggc agcatgggca cccctgcgcg caggtaatcc tccgtgcggt agagcgcgag
      481 cgaccagaag tgaggcggcg tccaggcaaa gatgattaga aacagcagca gcggctcgat
      541 cgtcacctcg ccggtcaccg ccgcccaccc gagcaccggc ggcatggcac ccgaggcgcc
      601 gccgatcacg atgttctgcg gcgtcgccgg cttgagcagc acagtgtaga tgatcgcgta
      661 gccgacgaaa gtggcgaggg tgagccacat cgtgagcgcg ttcacgtggt ggtggagaag
      721 ccaaaggccg aggccgccga gcgcgccggc gaacgcaagt gtctgcgccg ccgtgagctc
      781 gccctgcggc agcggccgcc aacgcgtgcg gctcatgact gcgtcgatcc tttgctccac
      841 caggcagttc accgccgccg cggcacccgc gaccagccag atgcctgccg tggccgcgag
      901 gagcgtgcgc aggggaacca tgcccggcgt ggcgaggaac atgccgatca ccgcagtgaa
      961 cacgatgagc gtcacgacgc gcggcttggt gagcgagtag aacgcacgca ggcgctgcgc
     1021 cgcgcttcgg ggaagaacgt gggtattcac tggcagagcg aaaagtctag cacgccatgt
     1081 ttcacccgaa ccgcgagtac cggagcagcc gctgcagatc cttgatcatc ctggagggat
     1141 ccgggtcgcg cgggaagcgc atcatcaggt tgcccagggg atcgacgagg tagatgtgat
     1201 cgacggtctt gcctggaaac tctttctccg aaagaaccgc gaggcgcgtg ccctcgatac
     1261 ccgggagtat ctcttcccgc ggcctggccc cgtcagtgat cacccagagc cgctccactc
     1321 ggctcatgtc cttgccctgg gcgcggcgca cctggcgcat gtagtacagc ttctgctcgc
     1381 agcgcgcatc gcacgcggag gcgtcgaaca tcacgagcac ccatttaccg cgcagcgcct
     1441 ccagccccgc aacgagcgtc ggcgtcagca gctcgccgta gttcgacggc gcgcccggcg
     1501 accagcgcag caggtatgcc ccggtgccga gcacaagcgg cgctgcgcac accagcgcga
     1561 cccacaaaag cttgttgcga aaaaggttca cctctttcga aacgacagca ccacgacgag
     1621 taccaccgca agcgcggcaa gcgaatacca ctgcagcgcg taggcttggt gcttctcgat
     1681 accggtatcg tgcggcggcc agtcgcgcac gaggccgtcg ggcaggaagg agtgctgctc
     1741 gatcacgcgc ggcgcgagcg caagcccagt ctccgcagcg taacctgcga catccaatgt
     1801 ctggcgcagc ttgccggaat tcttttcgat cgtgagaatc cgcggtagac gctcgagagc
     1861 gatgccctcg atgcgcacct cgccggcggg cgtcagcacc tctggtagcg cgtcgcgcgt
     1921 cgtaccagcg gcgagccagc cgcggttgac gagcacgtac gatggcgagc cgcgcagctt
     1981 gagcggcgtc acgacctcgt agcccggccg tccgcggcgt gtcttgttat cgaggtaaat
     2041 ggtgtgtttc gggacgaact cgccttcaac cgcgacgcgc tcgaggcgcg cagccgcagc
     2101 gcgcttttcg tcggcgcgcc cgcgttgcca gttgccgagc gcgatcgccg cggcgcacgc
     2161 cgcggcggcg agggcgagcg cccagagccg cggacggaaa gagtatcctg cgcccatgcg
     2221 ctacgtggtg atcgcgatat tcctagccat cgtcgcgagc ctcggctcgg cggcggtgtt
     2281 catgctgcgc ggccgcgggg actcgcgccg catggcccgc gccctcgcct ggcgcgtggg
     2341 cttgtcggtg gctctcttcg tgctcctgat tgcgggctat gcgctcgggc tgatccggcc
     2401 gggaaaccta tagccagtaa acgaagatga acaggccaag ccacactacg tcgacgaagt
     2461 gccagtacca cgccacgcct tcaaaggcga agtggtggtc gggcttgaag tggccgaaat
     2521 agcagcgaaa ccagatcacc gtcagcatga tcgcgccgat cgtgacgtgg aagccgtgaa
     2581 agcccgtcag catgaagaag gtcgagccgt agaccccggt gctcagcttg aggttgagat
     2641 cgctgtacgc gtggccgtac tcgtaagcct gcacgccgag gaaggtcacg cccagcagga
     2701 tggtgagcgc gagccatagg agaagcttcg gccggtcgtt gcggatgagc gcccagtgcg
     2761 cgaaggtgac ggtgacgccg gagctcagga gaagcgcggt gttgagcgca ggcaggcccc
     2821 aggcgcccat gatctggaac ttctcgatga agttcgggcc ggccgagggc cactggccgc
     2881 tgaagcccgg ccacatcagc gccttgtgct ccaggctcgc gaggtcgggc accgagtgga
     2941 tgcgcgccca gtagagcgcg ccgaagaacg cggcgaagaa catcacctcc gagaagatga
     3001 agaagctcat cccccagcgg aacgacacgt cctcccagct gccgtacttg ccgccctcgg
     3061 actcgcggat cacatcgccg aaccagcgca ccatcatgta gagcagcagc atgaatccca
     3121 cggcgatcga gacccag
//
LOCUS       DASTPD010000098        11328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_456470, whole genome shotgun sequence.
ACCESSION   DASTPD010000098 DASTPD010000000
VERSION     DASTPD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456470"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1321
                     /locus_tag="VFR57_03425"
     CDS             26..1321
                     /locus_tag="VFR57_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEU0258457.1"
                     /translation="MSAPVEEPKPEARVVEFPVAQTAGRAAYIAGTFDTKGRELFYLK
                     SCLEKLGLRTVTVDLATSGKPSSANIHPREVARHHPKGARAVFTNDRGSAVSEMALAF
                     EHFIGQRRDLGGLIAAGGSGGTALATQAMRRLPIGIPKVMVSTVASGDVKPYVGPSDI
                     CMMYSVTDVSGINRISEKVLSNAAHALAGMIAYRKDKTEETKPALGLTMFGLTTPCVQ
                     TVCKALEERYDCIVFHATGTGGQSMEKLFDSGLLAGVLDITTTEITDEIVGGVLTAGP
                     ARMDVFARRAMPYVGSCGALDMVNFWAMETVPPQFRERVLYRHNPNVTLMRTTPDEMT
                     RVGRFIAEKLNRMQGPVRFLIPEGGLSGIDAPGKPFWDPAADKALFDSLSQSFRATPS
                     RRLVRLPHHINDQVFAEALVANFNEIAQTAWPASRARTS"
     gene            1291..2127
                     /locus_tag="VFR57_03430"
     CDS             1291..2127
                     /locus_tag="VFR57_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEU0258458.1"
                     /translation="MARIPRQDILKKLHAQARRGEPIVGGGAGTGLSAKCEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLMAYGNANEIVLDMAREVLPVVKRTPVLAGVNGTDPFLI
                     VDSFLRKLIDLGFSGIQNFPTVGLFDGTIRAQLEETGMGYGLEVEVVRKAREMDLLTT
                     PYVFSEENARDMAKAGADIIVCHLGLTTGGAIGARTALGLEETIPKIESWSSAARSVK
                     PDVLVLCHGGPIATPEDASYVLRKAKGCNGFYGASSMERLPAEVALTETTRKFKAIGG
                     SL"
     gene            2124..3686
                     /locus_tag="VFR57_03435"
     CDS             2124..3686
                     /locus_tag="VFR57_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEU0258459.1"
                     /translation="MTGAQFGQLILALIGVAIIVALVVWLLNWLYLRSSKERGFVRTG
                     LGGQKVVMNGGAFVLPIVHEVLPVNMNTLRLEVSRGRERALITKDRMRVDVTSEFYVR
                     VKADADAIAAAAQTLGQRTLQPEQLKELVEGKFVDALRTVAAEMTMEEMHEKRGDYVK
                     RVRAAAAADLLQNGLELETVSLTQLDQTAMEFFNPSNAFDAEGLTRLTETIETRKKKR
                     NDIEQDTMIQIRNKNLDTERQALDIDRELEYARLTQERELEVRRAEQRAEVARQKAAS
                     EQDAERAQITAREAVERARIASERAVEQERIERERSVQESEISRRQAIELAEQQRAIA
                     VAEQSKAQSEAQAAADQARAKAVAEEEKVFTVREVELAQRKKLIDVSQAEAEADSSRI
                     RAMAAKIRHEVEAEGLTLMNKAANLLTPEARQSAMRRQLIDKLEGIIRESVKPMERIE
                     GIRVLHVEGLGGGQGGSNGSGNSNIADSVVNSALRFRAQAPLVDQLLREIGVQGGDLG
                     QAANALLEGKKP"
     gene            3683..4117
                     /locus_tag="VFR57_03440"
     CDS             3683..4117
                     /locus_tag="VFR57_03440"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0258460.1"
                     /translation="MIKVYTSSVIEAPADRVWAQVRDFNGLPQWTPFVAESRIENNLP
                     ADRIGCVRNFRLRDGGVIREQLLTLSDYDYQCSYSILESPMGVDNYVATLKLTPVTDG
                     NKTFAEWWAEFDCAPDKERELARNIGQGVFQAAFDSLKQKLR"
     gene            4197..4613
                     /locus_tag="VFR57_03445"
                     /pseudo
     CDS             4197..4613
                     /locus_tag="VFR57_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            4674..5594
                     /locus_tag="VFR57_03450"
     CDS             4674..5594
                     /locus_tag="VFR57_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU0258461.1"
                     /translation="MVATGFGGPEVLQLRKLEKPRGEVLIRQTAIGVNYIDVYIRKGL
                     YRMIEPPAPIGMEAAGIVVASESSRCRPGDRVAYACTPPGAYVTMRALPARDLVLLPD
                     NIDDGTAAAVMLKGMTAEYLLHRTHRVRPGDVVLVHAAAGGVGLLLCQWAKALGAHVI
                     GTVSSEDKAVLARANGCDQAIVTRDYRFAAQMKHRADVIYDGLGQAAARENFEALALR
                     GHWVSYGQASGPHDSFPDLTSKSGTLSRPVLFHYTADHLQEMAGNVFRALKEGVIRVD
                     ARHRYPLGAAADAHRDLEARRTTGQLLLLA"
     gene            5635..6513
                     /gene="panB"
                     /locus_tag="VFR57_03455"
     CDS             5635..6513
                     /gene="panB"
                     /locus_tag="VFR57_03455"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0258462.1"
                     /translation="MAAATSSERKKRTITEIRESKKTGEKMVYTSVLDYTSAKWAETA
                     GVDVCVVGDSLAMTCHGHPNTIPATMDMMVLHSQAVRRGAPNTFVLGCMPYQSYNTVD
                     RALTNATRFMQQAGCDAVKPQGGKSQAHILKALVDAGIPTASHIGLTPHTVAMFGGFR
                     IQGRTAEAAMKILEDAFAIQDAGCFMLEFEAVPAKIAKTISEQLEIPTIGIGAGVGTD
                     GQILLSYDLLGVFTDFKPKFTKRYTNLTEVAVNGLKQYVAEVKAGKFPDEDHSYGVDE
                     KEYDKFLSLVEKRKQR"
     gene            6449..7144
                     /locus_tag="VFR57_03460"
     CDS             6449..7144
                     /locus_tag="VFR57_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0258463.1"
                     /translation="MASTRRSTTNSSPSSRNESNAETLTDRAYTGIEELIVTLQLAPG
                     SAVSEQMLSERLGIGRTPIREALQRLARERLVTILPRRGVVVSAIDVAAQLRLLEVRR
                     ELERLVARGAARRATAAERGRFAELARRFEKAARTGDDKTFMRVDREFNELCLAAVRN
                     EFAAGAMGLMNSLSRRFWFHHYKQAADMPETAKLHADIARAIAEGDERGAAAALDRLV
                     DNIQAFTRATVDV"
     gene            7181..7987
                     /locus_tag="VFR57_03465"
     CDS             7181..7987
                     /locus_tag="VFR57_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU0258464.1"
                     /translation="MTKMVDRHAISERQKQFRAQYQAQIHPLYSGPVHIGVIYAVGLG
                     VIAWCVSRMHDATWEWLLVIPVFFFSNLFEWWIHKYVMHRLVDVWALRAIYDRHTRQH
                     HQYFTDNEMTVDSTREWRIIFFPWRALFTFMALGTPFALALGWAVNPNAGYILMVTII
                     GQYLLYETFHYCCHVRDNGFVRNLPFVNTIRRHHTAHHNYGIMMHVNMNLTFPIADWV
                     MGTSDLNRGLAGHIFNGYNEDHVKPELKPMIEKFRRHDAPVTIDGPIMQS"
     gene            8010..9065
                     /gene="dctP"
                     /locus_tag="VFR57_03470"
     CDS             8010..9065
                     /gene="dctP"
                     /locus_tag="VFR57_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0258465.1"
                     /translation="MKKRDFLKAGAGAAAALAALPVEAQQSGPTYTWKMATGWASGPL
                     MDIGAKGFAERMAQLSGGRFKIQTFPGGALGNALRVPETVKNGVAECGHTWMGYDWGK
                     DPTTVLFGGYAASMDSERMLHWMYDAGGDKMQRAYREEKEGVVSFPLFIRTAEAFLHS
                     RKPVRTLADLRGLKLRTAGAWLEMAKDLGAAPVTTAGGDVYPMLERGAIDATEWGTLW
                     ENITPGFHKVAKYVIIPGVHQPVAPFELVINKEAWAKVNAADQRLVELVAKLVTFETW
                     VKVGNEDAKALEFFRKAGNEIIELDVEVQRQTRKIGLEWARKTAQGNPWFQKVLQSQL
                     DFEKSWEGAERWRTIKA"
     gene            9068..9556
                     /locus_tag="VFR57_03475"
     CDS             9068..9556
                     /locus_tag="VFR57_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0258466.1"
                     /translation="MLRAIERASEWAGVLGACLLVPLVLATCYEVFARYLFGAPTIWA
                     YEVGYTLTGAHFLLGMAFTLKYGEHIRIDVFSGKFSPRTRAVIDLLGYAIVLPLTAWL
                     TLYLFFYLRTGYITNEKSGQSALNLPVWPLRVIFCLAFLLLALQVIAEVAKLLRALRK
                     EA"
     gene            9557..10876
                     /locus_tag="VFR57_03480"
     CDS             9557..10876
                     /locus_tag="VFR57_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0258467.1"
                     /translation="MEYLPFWMLPVAFTLMFLGFPVAFCMMITAVLFGLVVFGDRVVF
                     QFIEKIDDLSTNFVFAAVPLFVFMGSMLERSGIADRLFEAIHIWTRRMPGGLAIATII
                     MCILFAASSGVIGATESVVGLLTIPIMLRYGYDKGLISGTICAGGSLGTIIPPSVVAV
                     VIGPLADVSVGDILYGMTFPGLAMAGLYLVYIAGRCLINPAFAPAIPPSPDDPALAQK
                     LWITVRNLVPPVILIFAVLGSILLGWASPTEAAAIGAIASVGLTVLYGNFHLKSFYEA
                     LIKTLKVTAMIMTVLLAGTLFTGVFIGAGGIATANNLIGEMRLTPWMLLGLMMLIIFI
                     SGFFLDWISIVLIFVPIFTPLARTAGFDAVWFCVLFLVLIQTSYLTPPMAPAIFYLRA
                     VAPPAITISHMYRGVVPFIVLQLATLAITMAFPQLVTWLPTKMLQFR"
     gene            10890..>11328
                     /locus_tag="VFR57_03485"
     CDS             10890..>11328
                     /locus_tag="VFR57_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0258468.1"
                     /translation="MTPEQILSHKPRVLTQKQREFYFEEGYLLLEKIIPEEWLERLRA
                     ATDELIERSRKVTKADAVWDLEPGHRADAPRLRRVSAPVDQHPAYWDYVSQSLLGDIV
                     ADLVGPDVKFHHSKINFKWAHGGEEVKWHYDISFWPHTNYSPLT"
BASE COUNT         2021 a         3725 c         3680 g         1902 t
ORIGIN      
        1 cggcggcgtc aaccgctggg gtgtgatgtc ggcaccggtg gaagaaccga agcccgaagc
       61 gcgcgtcgtg gagtttccgg tggcgcagac ggccggacgt gccgcctata tcgcgggcac
      121 tttcgacacc aaggggcggg agcttttcta cctgaaaagc tgcctcgaga agctcggtct
      181 tcgcaccgtg accgtggacc tggcgacctc cggcaaaccc tcgtccgcaa acatccatcc
      241 gcgcgaggtc gcgcgccacc atccgaaggg cgcgcgcgcc gtcttcacca acgaccgcgg
      301 ctcggcggtg agcgaaatgg cgctcgcctt cgagcacttc atcggtcagc gccgcgacct
      361 cggcggattg atcgcagccg gcggatcggg cggcacggcg ctcgcgacgc aggcgatgcg
      421 ccggctgccg atcggcatcc cgaaggtgat ggtttccacc gtcgcctcgg gcgacgtaaa
      481 gccgtacgtc ggtccttccg acatctgcat gatgtactcg gtcaccgacg tctcgggcat
      541 caatcggatc tcggaaaagg tcctgtcgaa cgccgcccac gcgctcgccg gaatgatcgc
      601 ctatcggaaa gacaagaccg aagagacgaa gcctgcgctc ggcctgacga tgttcggcct
      661 caccacgcca tgcgtgcaga cggtatgcaa ggcgctcgaa gagcgctacg actgcatcgt
      721 gttccatgcc accggcaccg gcggtcaatc gatggagaag ctcttcgact ctgggttgct
      781 tgccggcgtg cttgacatca ccaccaccga gatcaccgat gaaatcgtcg gcggcgtgct
      841 gaccgccggg cccgcgcgca tggatgtttt cgcacgcagg gcgatgccct acgtgggctc
      901 ttgcggcgcg ctcgacatgg tgaatttctg ggcgatggaa acagtgccgc cgcaattccg
      961 ggagcgggtg ctttaccggc acaaccccaa cgtgacgctt atgcggacaa cgccagacga
     1021 gatgacgcgc gtcgggcgct tcatcgccga gaagctgaac cgcatgcagg ggccggtgcg
     1081 cttcctgatc ccggagggcg gattgtccgg aatcgatgca ccgggcaaac ctttctggga
     1141 tccggcggcg gacaaggcgc tgttcgattc gctttcacag agttttcgcg ccactccgtc
     1201 gcgccggttg gtccgcttgc ctcatcacat caacgaccag gtgttcgccg aggcgctggt
     1261 cgcgaacttc aacgagatcg ctcaaaccgc atggcccgca tcccgcgcca ggacatcctg
     1321 aagaagctgc acgcccaggc gcggcgcggc gagccgatcg tcggcggtgg cgccggcacc
     1381 ggcctttccg cgaagtgcga ggaggcgggc ggcatcgatc tgatcgtgat ctacaactcg
     1441 ggccgctacc gcatggcggg ccgcggctcg ctcgccgggc tcatggccta cggcaacgcg
     1501 aacgagatcg tgctcgacat ggcgcgcgaa gtgctgccgg tggtgaagcg cacgccggtt
     1561 ctcgccggcg tcaacggcac cgacccgttc ctcatcgtcg actcttttct gcgcaagctc
     1621 atcgacctgg gcttttccgg gattcagaac ttcccgaccg tcggtctttt cgacggcacg
     1681 atccgcgcgc agctcgagga aaccggcatg ggctacggcc tcgaggtgga agtcgtgcgc
     1741 aaggcgcgcg agatggacct gctcaccacc ccttacgttt tctcggaaga aaacgcgcgc
     1801 gacatggcga aggcgggcgc ggacatcatc gtctgccatc tcggcctcac caccggcggc
     1861 gcgatcggtg cgcgcaccgc cctggggttg gaagaaacca ttcccaaaat cgaaagctgg
     1921 tctagcgcgg cgcgcagcgt gaagccggac gtgctcgtgc tctgccacgg cgggcccatc
     1981 gcgacgccgg aagacgcttc ttacgtattg cggaaagcca aaggctgcaa cggcttctac
     2041 ggcgcctcga gcatggagcg cctgcccgcg gaagtcgcgc tcacggagac aaccagaaag
     2101 ttcaaggcca tcggaggttc cctatgacgg gtgctcaatt cggacagctc atcctcgccc
     2161 tcatcggcgt cgcgatcatc gtggcgctgg tggtgtggct tctcaactgg ctctacctgc
     2221 gctcctccaa ggagcgcggc ttcgtgcgca ccggactcgg cgggcagaag gtcgtaatga
     2281 acggcggcgc cttcgtgctg ccgatcgtgc acgaggtgtt gccggtcaac atgaatacgc
     2341 tgcggctgga ggtctcgcgc ggacgcgagc gcgcgctcat caccaaggac cgcatgcgcg
     2401 tcgacgtgac gagcgagttc tacgtgcgcg tgaaggcgga cgcggatgcg attgccgcgg
     2461 cggcgcagac gctcggccag cgcaccctgc agcccgaaca gctgaaggaa ctggtcgaag
     2521 gaaaattcgt cgatgcgctg cgcaccgtgg ccgccgagat gacgatggag gagatgcacg
     2581 agaagcgcgg cgactacgtg aagcgcgtgc gcgccgcggc cgcggccgat ctgctgcaga
     2641 acggcctcga gctcgagact gtgtcgctga cgcagctcga ccagacggcg atggagttct
     2701 tcaatccctc gaacgcgttc gatgccgaag gcctgacgcg gctcaccgag accatcgaga
     2761 cgcgcaagaa gaagcgcaac gacatcgagc aggacacgat gatccagatc cgcaacaaga
     2821 acctggacac cgagcgccag gcgctcgaca tcgaccgcga gctcgagtac gcgcgtctga
     2881 cgcaggagcg cgagctcgag gtgcggcgcg ccgagcagcg cgcggaggtg gcgcgccaga
     2941 aggcggccag cgagcaggac gccgagcgcg cgcagatcac cgcgcgcgag gcggtggagc
     3001 gcgcgcggat cgcgtccgag cgcgccgtcg agcaggaacg gatcgaacgt gagcgcagcg
     3061 tccaggaatc cgaaatcagc cgccggcagg ctatcgagct cgccgagcag cagcgtgcta
     3121 tcgcggtggc cgagcagtcg aaggcccagt ccgaggcgca ggccgcggcc gaccaggcac
     3181 gcgcaaaggc ggtggcggag gaggagaagg tgttcacggt gcgcgaagtc gagctcgcgc
     3241 agcgcaagaa gctgatcgac gtttcccagg ccgaggcgga agccgactcg tcgcgtatcc
     3301 gtgccatggc ggcgaagatc cgccacgaag tggaggccga gggcctgacc ctcatgaaca
     3361 aggccgccaa cctgctcacg ccggaagcgc gccagtcggc catgcgccgg cagctcatcg
     3421 acaagctcga gggcatcatc cgcgagtcgg tcaagccgat ggagcgcatc gagggcatcc
     3481 gggtgctgca cgtcgagggc ctcggcgggg ggcagggcgg gagcaacggc agcgggaaca
     3541 gcaatatcgc cgacagcgtc gtcaactcgg cgctgcgctt ccgcgcccag gcgccgctcg
     3601 tcgaccagct tctgcgcgag atcggcgtgc agggtggcga tctcggccag gcggcgaacg
     3661 cgcttttgga aggcaagaag ccttgattaa ggtctacacc tcgagcgtga tcgaggcgcc
     3721 cgcggatcgc gtctgggcgc aggtgcgcga cttcaacggc ctgccgcagt ggacgccgtt
     3781 cgtggcggag tcgcgcatcg aaaataacct gcccgcagac cgcatcggtt gcgtgcgcaa
     3841 ctttcggttg cgcgatggcg gcgtcatccg cgagcagctc ctcacgctct ccgattacga
     3901 ctaccagtgc agctactcga tcctcgagag cccgatgggc gtcgacaatt acgtcgccac
     3961 cctcaagctc actccggtca ccgacggcaa caagaccttc gccgaatggt gggccgagtt
     4021 cgactgcgcg ccggacaaag agcgcgagct cgcacgaaac atcggccagg gcgtttttca
     4081 agctgctttt gattcgctca agcaaaagct tcgttgaacc agatcagaaa tcggggtcag
     4141 agcccgattt cgggcaatcc gcctaaggaa aatcgggctc tgaccccgat ttgttcttga
     4201 ttcgcgtcag gcgctcggcc gtcatcgatg cgcccgtcga gcgcgtctgg gcggtgctgc
     4261 gcgacttcaa cagccactcc gcctggcacc cggcggtcgg cgactcgcgc atcgagcgcg
     4321 acgagccgtc cgaccaggtc ggctgcgtac gcaacttctt tctcaaggat ggcaaccata
     4381 tccgcgagca gctcctcgcg ctctcggatc gtgactacgt ctcgacctac tgcatcctcg
     4441 acgcgacgct cccgatgaaa aactacgtcg cgaccgtgca gttgaaaaga atcaccgacg
     4501 gcgagcgcac cttctggcac tgggaatcca ctttcgacgt acccaagggc cgcgagcgcg
     4561 agtttgagca gctcgtaggc ggcggggtct acgagaccgg ttttgcggcg ctcgcgcgct
     4621 acctcaatgg ggacggaccc cgggtgagac cccaggcggt ggagtcccaa ggcgtggtcg
     4681 ccacgggctt cggcggtccc gaagtcctgc agctcaggaa actggaaaag ccgagaggcg
     4741 aagtgctgat ccgccagaca gcgatcggcg tgaactacat cgacgtctac atccgcaagg
     4801 gtctctaccg catgatcgag ccgccggcgc cgatcgggat ggaggctgcg ggaatcgtcg
     4861 tggcgagcga gtcgagccgc tgccggcccg gcgatcgcgt cgcctatgcc tgcacgccgc
     4921 ccggagctta cgtcacgatg cgcgcgctgc ccgcgcgcga cttggtgctc ttaccagaca
     4981 acatcgacga cggaacggcc gcggccgtca tgttgaaggg catgacggcg gagtacctcc
     5041 tgcatcgcac gcatcgggtg cggccgggcg acgtggtgct ggtgcatgcg gccgcgggcg
     5101 gtgtcggcct gctgctctgt cagtgggcga aggcgctcgg cgcgcatgtc atcggcaccg
     5161 tgtcgagcga ggacaaggcc gtgctcgcgc gcgcgaacgg ctgcgaccag gccatcgtga
     5221 cgcgcgacta tcgattcgcg gcgcagatga agcacagggc cgacgtgatc tacgacggcc
     5281 tcgggcaggc agcggcccgg gagaacttcg aggcgctcgc gctgcgcggc cactgggtga
     5341 gctacgggca ggcaagcggg ccgcacgatt cttttccgga tctgacatcc aagtccggaa
     5401 cgctgtcgcg gccggtgctt ttccattaca ccgcggacca tctccaggag atggcgggca
     5461 acgtatttcg cgctctgaag gagggcgtga tccgcgtcga cgcgcgccat cgctatccgc
     5521 tcggcgcggc ggcagacgcg catcgcgatc tcgaggcgcg ccgcaccacc ggccagctgc
     5581 tgcttctggc gtagcctgca gtaacatatc agtggaatat caaacggagg gccaatggcg
     5641 gccgcgacga gcagcgagcg aaagaagcgc accatcaccg agatccgcga gtcgaagaag
     5701 accggcgaga agatggtcta cacctcggtg ctcgactaca cctcggccaa gtgggccgag
     5761 accgccggcg tcgatgtgtg cgtcgtcggc gactcgctcg ccatgacctg ccacggtcac
     5821 ccgaacacca tccccgcgac catggacatg atggtgctgc actcgcaggc ggtgcgccgc
     5881 ggcgcgccca acacttttgt tctcggctgc atgccctatc agagctacaa cacggtggac
     5941 cgcgcgctca ccaacgccac gcgcttcatg cagcaggcgg ggtgcgacgc ggtgaagccg
     6001 cagggcggca aatcgcaggc gcatatcttg aaggcgctgg tcgacgccgg catcccgacc
     6061 gcttcgcaca tcggcctcac gccgcacacc gtcgcgatgt tcggcgggtt ccgcatccag
     6121 ggccgcaccg ccgaggcagc gatgaagatc ctcgaggacg cttttgcgat ccaggacgcc
     6181 ggctgcttca tgctcgagtt cgaagccgtg cccgcgaaga tcgccaagac catctccgag
     6241 cagctcgaga tcccgaccat cggcatcggc gcgggcgtgg gcaccgacgg acagatcctt
     6301 ctttcgtacg acctcctcgg cgtgttcacc gacttcaagc cgaagttcac caagcgctac
     6361 accaacctca ccgaagtggc cgtgaacggc ctcaagcagt atgtcgccga agtgaaggcg
     6421 ggcaagttcc ccgacgagga tcacagctat ggcgtcgacg agaaggagta cgacaaattc
     6481 ctctccctcg tcgagaaacg aaagcaacgc tgaaacgctc accgaccgcg cctataccgg
     6541 gatcgaggag ctgatcgtca cgctgcagct cgcgccgggg agcgcggtgt cggagcagat
     6601 gctttcggag cgcctcggca tcggccgcac gcccatccgc gaagcgctgc agcgtctggc
     6661 gcgcgagcgc ctggtgacga tcctgccgcg gcgcggcgtg gtggtgtcgg ccatcgacgt
     6721 cgccgcgcag ctcaggctcc tggaggtgcg gcgcgagctg gagcgcctcg tcgcgcgcgg
     6781 cgcggcgcgg cgcgccacgg ctgccgagcg ggggcgcttc gcggagctcg cgcggcgctt
     6841 cgaaaaggcc gcgcgcaccg gcgacgacaa gaccttcatg cgcgtcgacc gagagttcaa
     6901 cgagctgtgc cttgccgctg tgcgcaacga gttcgccgcc ggcgccatgg ggctcatgaa
     6961 ctcgctttca cgccgcttct ggttccacca ctacaagcag gccgcggaca tgccggaaac
     7021 cgccaagctg cacgccgaca tcgcgcgcgc catcgccgag ggcgacgagc gcggggccgc
     7081 ggcggcgctc gaccggctgg tcgacaatat ccaggccttt acccgggcca ccgtcgatgt
     7141 ttaagattct gatatatcag taatgtgtca ggaggttgcg atgaccaaga tggtcgaccg
     7201 gcatgccatc agcgagcgtc agaagcagtt ccgtgcgcaa taccaggcgc aaatccatcc
     7261 tctttacagc gggccggtgc acatcggcgt gatctacgcc gtcggactcg gcgtgatcgc
     7321 gtggtgcgtg agccgcatgc acgacgccac ctgggagtgg ctgctcgtga tcccggtgtt
     7381 cttcttttcg aacctcttcg agtggtggat ccacaagtat gtcatgcatc gcctcgtcga
     7441 cgtctgggcg ctgcgcgcga tctacgaccg gcatacgcgc cagcaccacc agtacttcac
     7501 cgataacgag atgaccgtcg actcgacgcg cgagtggcgc atcatcttct tcccctggcg
     7561 cgcgctcttc accttcatgg cgctcggcac gccgttcgcg ctcgcgctcg gatgggcggt
     7621 gaacccgaac gcgggctaca tcctcatggt gacgatcatc gggcagtacc tgctctacga
     7681 gacgttccac tactgctgcc acgtgcgcga caacgggttc gtgcgcaacc tgcccttcgt
     7741 caacaccatc cgccgccacc acaccgcgca ccacaattac gggatcatga tgcacgtgaa
     7801 catgaacctc acgtttccga tcgccgactg ggtcatgggc acgagcgacc tcaaccgcgg
     7861 cctggccggg cacattttca acggctacaa cgaggatcac gtgaagccgg agctgaagcc
     7921 gatgatcgag aagttccgcc ggcacgacgc gccggtgacg atcgacggtc cgatcatgca
     7981 gtcctgaaga ctctccagag gaggagacga tgaagaagcg cgatttcctg aaagcgggcg
     8041 ccggcgcggc tgccgccctg gctgcactgc cggtcgaagc acagcagtcc ggcccgacct
     8101 acacctggaa aatggccacg ggctgggcga gcggcccgct catggacatc ggcgccaagg
     8161 ggttcgccga gcgcatggca cagctttcgg gcgggcgctt caagatccag acgtttccgg
     8221 gcggcgcact cggcaacgcg ctgcgtgtgc ccgagacggt aaagaacggc gtggccgagt
     8281 gcggccacac ctggatgggc tacgactggg gcaaggatcc gacaacggtc ctcttcggcg
     8341 gctacgcggc gtcgatggac agcgagcgca tgctgcactg gatgtacgac gcgggcggcg
     8401 acaagatgca gcgcgcctac cgggaagaga aggagggcgt ggtctccttc ccgctcttta
     8461 tccgcaccgc cgaggcgttc ctgcattcgc gcaagcccgt gcgcactctc gccgacctgc
     8521 gcggcctcaa gctgcgcacc gccggcgcct ggctcgagat ggcgaaggac ctcggcgcgg
     8581 cgccggtgac gacggcgggc ggcgatgtgt acccgatgct cgagcgcggc gcgatcgacg
     8641 ccacggagtg gggcacgctg tgggagaaca tcaccccggg attccacaag gtggcgaagt
     8701 acgtgatcat tcccggcgtg catcagccgg tggcgccctt cgaactcgtc atcaacaagg
     8761 aggcctgggc gaaggtcaat gccgccgatc agcgcctcgt cgagctggtc gcgaagcttg
     8821 tcacgttcga gacctgggtc aaggtcggca acgaggacgc gaaggcgctc gagttcttcc
     8881 gcaaggccgg caacgagatc atcgagctcg acgtcgaggt gcagcgccag acgcgcaaga
     8941 tcggcctcga atgggcgcgc aaaacggcgc agggcaaccc ctggttccag aaggtgctcc
     9001 agagccagct cgatttcgag aagtcctggg aaggcgcgga gcgctggcgc acgattaaag
     9061 cctgagcgtg ctgcgcgcga tcgagcgcgc gagcgagtgg gcgggcgtcc tcggcgcctg
     9121 cctgctcgtg ccgctcgtgc tcgccacctg ctacgaggtg ttcgcgcgct acctcttcgg
     9181 cgcgccgacc atctgggcct acgaggtcgg ctacacgctc accggggcac atttccttct
     9241 cggcatggcg ttcacgctca agtacggcga gcacatccgc atcgacgtct tcagcggcaa
     9301 gttctcgccg cgtactcgcg cggtgatcga tcttctcggc tacgcgatcg tgctgccgct
     9361 cacggcgtgg ctcacgctct atctcttctt ttacctgcgc accggctata tcaccaacga
     9421 gaaaagcggc cagtcggcgc tcaacctgcc ggtctggccg ctgcgcgtca tcttctgcct
     9481 ggcattcctg ctcctcgcgc tgcaggtgat cgcggaagtg gccaagctcc tccgcgccct
     9541 gcgcaaggag gcgtagttgg agtacctgcc gttctggatg ctgccggtgg cgttcacgct
     9601 gatgttcctc ggctttccgg tggcattctg catgatgatc accgcggtgc tcttcggcct
     9661 cgtggtgttc ggcgaccgcg tggtgttcca gttcatcgag aagatcgacg atctctcgac
     9721 caacttcgtg ttcgccgcgg tgccgctctt cgtcttcatg ggatcgatgc tagaacgctc
     9781 cggcatagcc gatcgcctgt tcgaggcgat ccacatctgg acgcgccgca tgcctggcgg
     9841 cctcgccatc gcgactatca tcatgtgcat cctgttcgcg gcgtcgtcgg gcgtgatcgg
     9901 cgccaccgaa tcggtggtgg gcctcctcac catcccgatc atgctgcgct acggctacga
     9961 caaggggctg atcagcggaa cgatctgcgc cggcggctcg ctcggcacga tcattccgcc
    10021 gtcggtggtc gcggtcgtga tcggaccgct ggccgatgtg tcggtcggcg acatcctgta
    10081 cggcatgacg ttcccgggcc tggctatggc gggcctctac ctcgtctaca ttgctggacg
    10141 ctgcctgatc aacccggcgt tcgcgccggc gatcccgccg tcacccgacg acccggcgct
    10201 cgcgcagaag ctctggatca ccgtgcgcaa ccttgttccg cccgtgatac tgatcttcgc
    10261 ggtgctcggc tccatcctgc tcggctgggc ttcaccgacg gaggcggcgg caatcggcgc
    10321 catcgcctcg gtcgggctca cagtgctcta cggcaacttc catctcaagt cgttctacga
    10381 ggcgctcatc aagacgctca aagtgacggc gatgatcatg acggtgttgc tcgccggcac
    10441 gctttttacc ggcgtgttca tcggcgcggg cggcatcgct accgccaaca acctcattgg
    10501 cgagatgcgc ctcacgccgt ggatgctgct cggcctgatg atgctcatca tcttcatctc
    10561 ggggttcttc ctcgactgga tctcgatcgt gctcatcttc gtgcccatct tcacgccgct
    10621 cgcgcgcacc gccggattcg acgcggtctg gttctgcgtg ctttttctgg tactgatcca
    10681 aacgagctac cttacgccgc cgatggcgcc cgctattttc tacctgcgcg cggtggcgcc
    10741 gccggcgatc accatcagcc acatgtaccg cggcgtcgta ccgttcatcg tgctgcagct
    10801 cgccacgctc gcgatcacca tggcattccc gcagctcgtc acgtggctgc cgaccaagat
    10861 gctccagttc cgctgaagga gactcatcca tgacgcccga acagatcctt tcccacaagc
    10921 cgcgcgtgct gacccagaag cagcgcgagt tctacttcga ggagggttat ctgctcctcg
    10981 agaagatcat cccggaggag tggcttgagc ggctgcgcgc cgcgaccgac gaattgatcg
    11041 agcggagccg caaggtgacg aaggccgacg cggtatggga cctggagccc ggccaccgcg
    11101 ccgacgcgcc gcggctgcgc cgcgtctcgg cgccggtcga tcagcatccg gcgtactggg
    11161 attacgtgtc gcaatcgctg ctcggggaca tcgtcgccga tctcgtcggt cccgatgtga
    11221 agttccacca ctccaagatc aacttcaagt gggcgcacgg cggcgaggag gtgaagtggc
    11281 actacgacat ctcgttctgg ccacacacga actactcgcc gcttaccg
//
LOCUS       DASTPD010000099         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_663332, whole genome shotgun sequence.
ACCESSION   DASTPD010000099 DASTPD010000000
VERSION     DASTPD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663332"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFR57_03490"
     CDS             <1..651
                     /locus_tag="VFR57_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258469.1"
                     /translation="IPIVMAVVGDPVKLGLVASFRQPGGNVTGLTLVGAERTEKQLQL
                     LKEILPGTSTVAVLYNAANPSTPDGLADARRAASLLKFSLQLVPVRGPEDLETAFAQI
                     INARSNAFVTLGDGMLLGSHRRIAEFGVRSRLPGVFPEREFADAGGVASYGPNVAANF
                     RRAAGFVASILKGAKPSDLPVEQPTKYDVVINMKTAKAIGLKVPPAVLVRADEIIE"
     gene            complement(690..1307)
                     /locus_tag="VFR57_03495"
     CDS             complement(690..1307)
                     /locus_tag="VFR57_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0258470.1"
                     /translation="MKLLASLASPYTRKVRIVLAEKKIDCDMDLVDVAPVDNPVNRHN
                     PLGKIPTLLLDDGTALYDSRVIVEFLDGKSPISRLIPDDLRDRVAVRRLEALADGVLD
                     AGLLVRYESLRAEHERSQAWTDKQLARMKRGMAQMASEVAERPWCHGERYSLADIALG
                     CCLGWLDFRKPGGVDWLAEYPGLARHYEKLKARPAFADTVPQVPR"
     gene            complement(1319..1918)
                     /locus_tag="VFR57_03500"
     CDS             complement(1319..1918)
                     /locus_tag="VFR57_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU0258471.1"
                     /translation="MGPYYKGMRISLSALSREARACTECAQELPFKPRPVFRVGRRAR
                     LLIVGQAPGRLVHETGIPWNDPSGEVLRAWLALDREAFYDTSRIAIVPMGLCYPGTVD
                     GADLPPCRECAPLWQPRFRAALPDIRLTLLVGAYAQAFYLRARRKKTLADTVRAWRDY
                     LPEFFPLPHPSWRNIGWRARNPWFEKDVLPALRQCVRTL"
     gene            1917..>2214
                     /locus_tag="VFR57_03505"
     CDS             1917..>2214
                     /locus_tag="VFR57_03505"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0258472.1"
                     /translation="MPSSLAALSPLDGRYSSGADPLRGYFSEQALIRYRTRIELEWLQ
                     ALAAERAIKELKPFATRTLSEFSRLLRNFSEQDAGEIKAIEAKTNHDVKAIELWL"
BASE COUNT          379 a          701 c          756 g          378 t
ORIGIN      
        1 ataccgatcg ttatggcggt cgtcggagac ccggtcaagc tcggcctggt ggcgagcttc
       61 cggcaaccgg gaggaaatgt cactggcctc accctggtgg gtgccgagcg cactgagaag
      121 caactgcagc tgctaaagga aatcttgcct ggcacgtcca ccgtcgcggt tctctacaac
      181 gccgcaaatc cttcgacgcc ggacgggttg gcagacgcgc gtcgcgcggc gtccctcctg
      241 aagttttcgc tgcagctcgt gccggtgcgc ggcccggagg atcttgagac tgcatttgcg
      301 cagattatca atgcgcgatc caacgctttc gtcactctcg gcgacggtat gctcctgggt
      361 agccatagac ggatcgctga attcggcgta cgcagccgcc tgccaggcgt atttcccgag
      421 cgcgagttcg ctgacgccgg aggggtagcg tcctacggtc cgaacgtcgc agccaatttt
      481 cggcgcgccg ccggctttgt tgcgagcatt ctcaaaggcg ccaaaccgag cgatctgccc
      541 gtcgagcagc cgacgaagta tgacgtcgtc atcaacatga agaccgcgaa ggctatcggg
      601 ctcaaggtcc cgccagccgt gctagtgcgc gcagacgaga tcatcgagta gcctgcggcc
      661 gcaattgggg tctgaccccg attttcggtt tacctgggta cctgcgggac ggtgtcggcg
      721 aaggccggcc gggccttcag cttttcgtag tgcctggcga gccccgggta ctcggccagc
      781 cagtcgactc cgccgggctt gcgaaagtcg agccagccga ggcagcagcc gagcgcgatg
      841 tcggcgagcg agtagcgctc gccgtggcac cacggtcgtt cggcgacctc ggaggccatc
      901 tgcgccatgc cgcgcttcat ccgcgcgagc tgcttgtcgg tccaggcctg actgcgctcg
      961 tgctccgcgc gcagcgattc atagcgcacg agaagccccg catcgagcac gccgtcggca
     1021 agtgcctcca ggcggcgcac ggcgacgcga tcgcgcaggt cgtcggggat caggcggctg
     1081 atgggcgact tgccgtcgag gaattcgacg atcacgcgcg agtcgtagag tgccgttccg
     1141 tcgtcaagca ggagggtggg aatcttgccg agcgggttgt gccggttgac cgggttatcg
     1201 accggggcga cgtcgaccag atccatgtcg caatcgatct tcttctcggc gagcacgatg
     1261 cgcaccttgc gggtataggg gctggcgagc gaggctagga gcttcatgaa gggcgcgatt
     1321 ataaggtccg cacgcactgg cgcagggcgg gaagcacgtc tttctcgaac cagggattgc
     1381 gcgcgcgcca cccgatgttg cgccagctcg ggtgcggcag ggggaagaac tcgggcaggt
     1441 agtcgcgcca cgcccgtacg gtgtcggcga gcgttttctt gcgccgcgcc ctcaggtaga
     1501 acgcctgcgc gtaggcgccg acgaggaggg taaggcggat atcgggcagc gccgcgcgga
     1561 agcgcggctg ccacaggggc gcgcattcgc ggcacggcgg cagatccgcg ccgtccaccg
     1621 tgccgggata gcaaagcccc atcggcacga tcgcgatgcg cgaggtgtcg tagaacgctt
     1681 cgcggtcgag cgcgagccat gcgcgcagca cttcgcccga gggatcattc cacgggatgc
     1741 cggtttcgtg cacgagccgc ccgggcgcct ggccgacgat aaggagacgc gcgcgcctgc
     1801 cgacgcggaa cacggggcgc ggcttgaacg gcagctcctg cgcgcactcg gtgcaggcgc
     1861 gcgcttcgcg gctcagcgcg gagagtgaaa ttcgcatccc cttataatag ggccccatgc
     1921 cttcgtcgct cgccgcgctg tcgcctctcg acgggcggta ctcaagcggc gccgatcccc
     1981 tgcgcggtta cttcagcgag caggcgctca tccgctaccg cacgcgcatc gagctcgaat
     2041 ggctccaggc gctcgccgcc gagcgcgcga tcaaggagct caagcctttc gcgacgcgca
     2101 ccctatccga gttttcgcgg ctgctaagaa atttttcgga acaggatgcg ggcgagatca
     2161 aggcgatcga ggcgaagacc aatcatgacg tcaaggcgat cgagctttgg ctga
//
LOCUS       DASTPD010000100         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1683999, whole genome shotgun sequence.
ACCESSION   DASTPD010000100 DASTPD010000000
VERSION     DASTPD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683999"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFR57_03510"
     CDS             complement(<1..342)
                     /locus_tag="VFR57_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU0258473.1"
                     /translation="MYSPAYNRLEDRAELLEFMRANSFVVLVTGTGGTLHASHLPAMV
                     HDDNGAIRVDMHMARANPQWREFFDDEEVLVVFPGPHAYISPRWYEEQERVPTWNYAA
                     VHAYGTPKVIDD"
     gene            complement(342..809)
                     /gene="ispF"
                     /locus_tag="VFR57_03515"
     CDS             complement(342..809)
                     /gene="ispF"
                     /locus_tag="VFR57_03515"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU0258474.1"
                     /translation="MRIGQGFDVHPLVAGRKLIIGGVEIPYHKGLEGHSDADVLLHAI
                     CDAILGAAGLGDIGGHYPDSDTAFAGADSRGLLRDVAQKLGAKKLKVLNIDATILAQA
                     PRMAPHMPRMVANIAADLGIEPAAVNIKATTTEGLGFVGRLEGIAAQAVALLD"
     gene            complement(806..1501)
                     /gene="ispD"
                     /locus_tag="VFR57_03520"
     CDS             complement(806..1501)
                     /gene="ispD"
                     /locus_tag="VFR57_03520"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU0258475.1"
                     /translation="MSIYALIPAAGSGTRFSGLAPKQYLPLAGRPMLWHAVRAVCVPP
                     VESVFVVLAPSDSAFATHDWSAFAGKLEPLYCGGETRRDSVYNGLVAAMAAVEANDWM
                     LVHDAARPCLPRKDFDTLLRETQHDEVGGILALPIADTVKKAAKDESGAQRVAATEDR
                     SVLWLAQTPQIFRAGLLAQALQRARGAPTDEAAAVEQLGLRPRLIAGSRENLKVTWPE
                     DITLAESILKGRT"
     gene            1551..>2550
                     /locus_tag="VFR57_03525"
     CDS             1551..>2550
                     /locus_tag="VFR57_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0258476.1"
                     /translation="MHRDSLPARRRYTRLAGSADALALARLAQTEKPLAVITAGALDA
                     QRLLEELRWFAPQLRLCLLPDWETLPYDQFSPHQDLVSERLATLYRIQRGDFDVAVMP
                     AATALVRLCPPSYIAGHTFFLKAREKLSLERVRQQLAVAGYQHVTQVLSPGEVCFRGG
                     LIDLFPMGSALPYRIDLDDDLIDAIKTFDVDTQRTLYSVNEIRMLPAREFPLDDAGRA
                     RFRSRWRELFEGDPSKKRLYRDVSNGVAAAGIEYYLPLFFDSVATLFDYLPAASVLAL
                     HHEVADAIQEFWRDANTRHKMAGGDPDRPLLAPAQLFVPAEEFYIRAQAFPRVDILD"
BASE COUNT          407 a          876 c          806 g          461 t
ORIGIN      
        1 gtcgtcgatg actttcgggg tgccgtacgc gtgcaccgcg gcgtagttcc acgtcggcac
       61 ccgctcctgc tcttcgtacc agcgcggcga gatgtaggca tgcgggccgg gaaagacgac
      121 gagcacctct tcgtcgtcga agaactccct ccactgcggg ttggcgcgcg ccatgtgcat
      181 gtcgacacgg atcgcgccgt tgtcgtcgtg caccatcgcg ggcagatggg aggcgtgcaa
      241 cgtgccgccg gttccggtga cgagcacgac aaaactgttg gcacgcatga attccagcag
      301 ctccgcgcgg tcttcgagcc ggttataggc gggagagtac attagtcgag aagcgccacc
      361 gcctgcgccg cgatgccttc caggcgcccg acgaagccca ggccttcggt cgtcgtcgcc
      421 ttgatattga ctgccgccgg ctcaatcccg agatcggcgg cgatgttcgc gaccatgcgc
      481 ggcatgtgcg gcgccatgcg cggcgcctgc gcgagaatcg tggcgtcgat gttcaacacc
      541 ttcagcttct tcgcaccgag cttttgcgcc acgtcccgta ggaggccccg gctgtcggcg
      601 cccgcgaacg ccgtgtcgga gtctggatag tgcccgccga tatcgccgag gccggcggcg
      661 ccaaggattg cgtcgcagat cgcgtgcagc aggacatcgg cgtcggaatg accctccagc
      721 cccttgtggt acgggatctc gacgccgccg atgatcagct tgcgccccgc gacgagcgga
      781 tgcacgtcga agccctgccc gatcctcatg tacgaccctt caggatgctc tccgcgagcg
      841 tgatgtcctc cggccacgtg accttgaggt tttcgcggct gccggcgatt aaccgcggcc
      901 gcaggcccag ttgctcgacc gcggcggctt cgtcggtcgg cgcgcccctc gcacgctgca
      961 gggcctgagc aagcaagccg gcgcggaata tctggggagt ctgggccagc cacaacacgg
     1021 aacggtcctc tgtagcggca acgcgttgcg cgccgctttc atccttggcg gcttttttca
     1081 cggtatcggc gatcggcagc gccaggattc cacccacctc gtcgtgttgc gtctcccgca
     1141 gcagagtatc gaaatccttg cgtggcaggc agggccgcgc ggcatcgtgc accagcatcc
     1201 agtcattcgc ttccaccgcc gccattgccg ccaccagccc gttgtacacc gaatcgcggc
     1261 gcgtttcgcc gccgcagtag agcggctcga gcttgccggc gaaggcgctc cagtcgtgcg
     1321 tcgcgaacgc gctgtcgctg ggtgcgagca ccacgaacac cgactccacc ggcggcacgc
     1381 agacggctcg caccgcatgc cacagcatcg gccggccggc gagcggcagg tattgcttcg
     1441 gcgccaagcc cgaaaaccga gtaccgctcc ctgcggcggg aatcaacgcg tagatggaca
     1501 tagaagttct tttaattcag gccgatataa ttgcataggt cacgcttctg atgcaccgcg
     1561 attcgcttcc cgcccgccga cgttataccc ggctcgccgg ctcagccgac gcgcttgcgc
     1621 tcgcacgtct ggcgcagacg gagaaaccgc ttgcggtcat tacagcaggc gcgctcgacg
     1681 cgcagcggct gctcgaggaa ctgcggtggt tcgcaccgca gttgcgcctg tgtctgctcc
     1741 cggactggga aacgcttccc tacgaccagt tctcgccgca ccaggacttg gtgtcagagc
     1801 ggctggccac gctttacagg atccagcgcg gcgacttcga cgtcgcggtg atgccggcgg
     1861 cgacggcgtt ggtgcgactg tgcccgccgt cctatatcgc cggccacact ttcttcctca
     1921 aggcgcggga aaagctctcc ctcgagcggg tcaggcagca actcgccgtg gccggctacc
     1981 agcatgtcac gcaggtctta tcgccgggag aagtgtgctt tcgcggcggc ctgatcgatc
     2041 tgttcccgat gggatcggcg ctcccctacc gcatcgacct cgacgacgac ctgatcgacg
     2101 ccatcaagac gttcgacgtc gacacgcagc gcacgctgta ctcggtgaac gagatccgca
     2161 tgctgcccgc gcgcgagttc ccgctcgatg acgccggccg cgcgcgtttc cgcagtcgct
     2221 ggcgcgagct tttcgagggc gacccgtcga agaagcgact ctaccgtgac gtttccaacg
     2281 gcgtggcggc cgcggggatc gagtattacc tgccgctgtt cttcgactcc gtcgcgacgc
     2341 tgtttgatta cctgcccgcc gccagcgtcc tcgcgctgca ccacgaggtc gccgatgcca
     2401 tccaggagtt ctggcgcgat gcgaacacgc gccacaagat ggccggcggc gatcccgacc
     2461 ggccgctcct ggcgcccgcg cagctgttcg tcccggcgga ggagttctac atacgcgctc
     2521 aggcctttcc ccgggtcgac atactggata
//
LOCUS       DASTPD010000101        20488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_255396, whole genome shotgun sequence.
ACCESSION   DASTPD010000101 DASTPD010000000
VERSION     DASTPD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255396"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..679)
                     /locus_tag="VFR57_03530"
     CDS             complement(191..679)
                     /locus_tag="VFR57_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0258477.1"
                     /translation="MTKLGSMNHLRLTVSDIPRAERFYSVVLGFLGYRLVERSPFRIA
                     YAAPTPSGNLQWMIFSLLRKGSSSKHDRYSTGFHHLALNADTREQVDALHDVLQKIGA
                     TVLDAPADYDFEPGYYAVFFADPDGMKLELVHVPPEGSQHYWRERERNGVVELDHLPP
                     GY"
     gene            complement(676..1677)
                     /locus_tag="VFR57_03535"
     CDS             complement(676..1677)
                     /locus_tag="VFR57_03535"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEU0258478.1"
                     /translation="MANPIESLPRVALAALPTPLQPLKRLSAALGGPEIWVKRDDLTG
                     AGGGGNKVRKLEFLLAEALAQKADVVVTGGAIQSNHVRQTAALCGALGLACEGVLNEP
                     LPGRGPEYTVSGNVFLTRLADATVHVIPNTADTAEYMKRVAQGSRAAGKRPYVMPVGG
                     STPLGAVGYANAAMELCEQMDDFDTIVLATGSGGTQAGLLGGLALEGSAARVLGFSVG
                     APLERQRGKVFDLLPGLAELLGVELPEDAVIVDDQSVGPGYGVPTPEVKEALAMAASL
                     EGLYLDPVYTGKSMAGLIRHARAGRFRAGEKVVFLHTGGLPGLFGYATELSAGAKKR"
     gene            complement(1714..3846)
                     /locus_tag="VFR57_03540"
     CDS             complement(1714..3846)
                     /locus_tag="VFR57_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0258479.1"
                     /translation="MLTDLPEPLRERCDYIYSRLKIALVATLVLSFFFSAFLWRARPV
                     ELVAFWQGVMVLLAAALWMIGWAYRRSQGRENPEAWLRRAAFAAGMLGAGWGFAAAVF
                     FPGSQAEHALISFVVALVAAAGLPLFSTVWWVYAAYGAAVMLPFAVVVFAHGTEFFRL
                     FGAAVPLLYAAYVATAWHLGKAFSTAYGLRSAYDRLSNDNAAVQAQLAEQLDSLLDAH
                     REVQAYGRKLSLFSERAPIAVLELDPRATILDINPAAENLFGYAAPEMVGRNLFTMLL
                     PADDRARTEHWWTTMVQEGKPATMTAERCLRRDGLELACEWTVTPLVNDANDVTSIVL
                     HGRDITQQRASERVRSEFTSTLSHELRTPLTSILGSLQLVQSGALGELAAEQAELISV
                     AERNGQRLLDLINEVLDIEKIESGRLALVPEPIALKGLLEESLRLNQGYADRFKVHLA
                     LVGKPPDVTVRADRKRLMQAMTNFLSNAAKFSPPDGTVEVSARLEGDGVRVEVGDRGP
                     GIPEKFRSRIFGRFAQADSADSRIKGGTGLGLSISKRLVEMMQGRVGFEDRAGGGTTF
                     FLALPVMKAGAETADNAVRVLLTEYDSVTAEYLAMVLDKGGYHVDTAADAAASRELLS
                     RWEYAAWLVSRRLPGGEDPVTLVAELRARLHNTKVIVLVGGQNEQAEAPGAAGNGIVD
                     CVAKNDSRANILEAVEKWIGRVAPQPGT"
     gene            3887..5092
                     /locus_tag="VFR57_03545"
     CDS             3887..5092
                     /locus_tag="VFR57_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU0258480.1"
                     /translation="MVPVPELEFGMYVAELDRPWTETPFQFQGFFLQTREQLETLKKF
                     CKTVLVDLDRRETVERLPKPPAGGASSALDLSRTGKKKYAEVAPVEHEYARASATHAA
                     GMAVVNDAFAALRAGKTLEANRVNEAVNGITESVMRNADALLLFTQLQSKGDYTQSHA
                     LDVSVYMTAFGRFLEMSADDIRLLGHVGLLQDVGKVRLPDGLIEKRERLTREEFELAK
                     SHVVHSAEILNATPGLPPALAELAFLHHERHDGSGYPKGLRGRDIGLVGSIAGIVDTF
                     DALTARRPYGEPVAPSTALGMIYKWRGTLFDAYVVEQFIRFVGIFPLGSLIELNSGEV
                     GIVITQNLDLRLQPRVMVIRDANGNPLKPQKLVDLSRGHKLGSGELYRIKRTLEYGRI
                     PVSAESLFS"
     gene            complement(5136..6710)
                     /locus_tag="VFR57_03550"
     CDS             complement(5136..6710)
                     /locus_tag="VFR57_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258481.1"
                     /translation="MVHVRIASFAVSLVFAGGVHAQGVTGLGKVNFPNSCSPAVQEKL
                     QNGVAMLHSFYYTAAKRTFEEVAQADSSCAIAAWGFASILMSNPLAGQGATPPDAEAA
                     QTAIDKGRRMGAKTERERDYLEAVAAYYQDFGKRSERERQVARANAYEALAAKYPKDD
                     EAQIFYALYLAGTQLQTDQTYAAYLKAAKILEAQFKKYPQHPGVAHYLIHSYDAPPIA
                     KQGIPAARRYAKIAPEAPHALHMPSHIFTRVGEWADSAATNMRSASAAEKNNEHDEAA
                     HALDYAVYAYLQLARDSEARKVTEHALGFKNFTPRFVGPYAVAAMSARVALERADWQA
                     AARLEPRQDKFPFTVALTHFARALGAARSGNPESAQKDAERLAALGEQLKEAKNAYWA
                     GEVEVMRLSSVAWIARAQGKNEEALETMRRAADLEDKSEKHIVTPARLLPARELLGDM
                     LLELKRPADALKEYEASHIREPNRLRGYYGAGLAASQAGQQAKAKQHYTRLVALAGKG
                     EPRPEVAAARTWLGAH"
     gene            complement(6803..7063)
                     /gene="minE"
                     /locus_tag="VFR57_03555"
     CDS             complement(6803..7063)
                     /gene="minE"
                     /locus_tag="VFR57_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEU0258482.1"
                     /translation="MGSFLSFLLGQRKTSATVAKERLQIILAHERTGRRRHAGYLSSL
                     QRDLIDVICKYTKIDPKDIKVNLERQQNLDVLEVKIELPEAR"
     gene            complement(7070..7885)
                     /gene="minD"
                     /locus_tag="VFR57_03560"
     CDS             complement(7070..7885)
                     /gene="minD"
                     /locus_tag="VFR57_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEU0258483.1"
                     /translation="MARIVVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLVNVIQGEANLHQALIKDKQCDNLSVLAASQTRDKEALTKEGV
                     DKVLTGLADMGYEYIVCDSPAGIETGAMMAMHFADEALVVTNPEVSSVRDSDRILGML
                     ASKTKRAIQGGEPVKEHLLITRYTTQRVHSGEMLSLDDIQDILRIKLLGVIPESETVL
                     QSSNQGVPAVHMKGSDVSEAYKDMVARFLGEEKPLRFVNGYKPSFFKRLMGGK"
     gene            complement(7920..8735)
                     /gene="minC"
                     /locus_tag="VFR57_03565"
     CDS             complement(7920..8735)
                     /gene="minC"
                     /locus_tag="VFR57_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEU0258484.1"
                     /translation="MTLAVQGTTPETFEIKSATLPLVALVLKSPDLAALAHELEARFG
                     DIQDFFDQDAMVIDLSRLESEAPEGERPAVDFSALIELLKKYRVVPVGVHGGSAEQTA
                     AALAAGLYPAPDARIGKAAPGVSSAELPAQAAPEPASQPALQPVSQPVPQPAAAPTGA
                     LVIDKPMRSGQQAYARGRDLVMLAMVNPGAEVIADGHIHVYAPLRGRAIAGARGNTDA
                     RIFALSMEPELVSIAGLYRTGEAPLLAAVWGKPTQVRLASNSEGNRLLFEPLK"
     gene            8917..9909
                     /locus_tag="VFR57_03570"
     CDS             8917..9909
                     /locus_tag="VFR57_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258485.1"
                     /translation="MSEIRLEEVTKRFGAVRAVDAVSFGAQKGKFVVLLGPSGCGKST
                     LLRLIAGLEEVSEGRIFIEDRDVTNLDPTKRRISMVFQSYALFPHLTVAENIVFGLRV
                     RRTDAAERERRLNTVAELVGLGERLQHKPQQLSGGQRQRVALARAIVSQNPICLMDEP
                     LSNLDAQLRHDMRVEIRALQRRLGMTVVYVTHDQVEAMSMADHVVLLREGRIEQEGSP
                     AELYARPATTFAARFIGTPAMNLVPLHAIQAGATANLTAGIRPEHIRLGDGIPATVLS
                     AEYHGADTIVTAQVNGSALLVRAPGQVDLAAGSQVKLGWEPGAVHIFDSEGRKT"
     gene            9906..11174
                     /locus_tag="VFR57_03575"
     CDS             9906..11174
                     /locus_tag="VFR57_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258486.1"
                     /translation="MKRFLGLILAAAFAATAYAQKTELTFYYPIAVGGPLTKVIDGYA
                     AGFEKENPNIKVNAVYAGNYDDTRIRALAAHKAGQPAQLSVLFSIDLYELLEQDLIVP
                     FDDMAKTPEDKAWLKSFYPALMTNGTYKGKTYGIPFQRSTIVLYWNKAAFKEAGLDPE
                     KPPTNWNEMRDMAAKLVKKDGGNVTRWGVMVPSTGYPYWMFQAFSRQNGQDLMNPEGN
                     RTNFTHPDVVAALQYWVDLGRKEGVMPAGTVEWGTLRQNFLEGKTAMMWHTTGNLTAV
                     KDGAKFPFGVAMLPASKQRGSPTGGGNWYLFKKSTPQEREAAFKFIKWITAPERAADW
                     SIATGYVAVSPAAYETPKLKSYVSEFPPAAVARDQFKFATPELSTFQTGRVRKSLDDA
                     IQAALTGSKSPADALKTAQTESERLLKPYK"
     gene            11202..12065
                     /locus_tag="VFR57_03580"
     CDS             11202..12065
                     /locus_tag="VFR57_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0258487.1"
                     /translation="MKHVYGWLLLTPAAILLVAFTHYPAVATLADSFFSHATALRPAR
                     FVGVDNYAALAGDPVFWQVLKNNFWFALGTIPTSIALALVMALWVNARLPGRALVRLA
                     YFTPTVLPMIAVANLWLYFYTPQIGLLDQVTGLFGAPSHNWLGNPDTVMGCVIAMTIW
                     KEAGFFMIFYLAALQTLPPELEEAARIEGASRGYFFWRVTFPLLMPTTLFVLVTATIN
                     SFKLVDHLFILTKGGPDNASNLLLYYIYQVAFSYLDTAYAATLTVILLFLLAAFAVLQ
                     WQMIERRVHYR"
     gene            12069..13250
                     /locus_tag="VFR57_03585"
     CDS             12069..13250
                     /locus_tag="VFR57_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258488.1"
                     /translation="MAIAQRWRILGALTFARTSIGFQFQSVAPLAPLVSGELGLDNTE
                     LGWLIGLYLLPGVVAALPGGLLGARYGDKRMTLLGLALMAGGGIWLASAASFAEADAA
                     RLVSGIGAVVLNVLLTKMVADWFEGKERLLAMSVLINAWPIGIGLALLALPLIGEATG
                     WRGAIAAAALLAAAGFTAVLFFYRAPAAAQAGPDGGVGLNALDAKEWRLLAVASIPWL
                     AFNAAYQLVVSFLPSFLLETGSSLTFSGALTALNTALVIVSVQAGGVLLKRSRRPDLL
                     CHVAIVVWCASLLLLASESAPLLWIVAGGLIAGIPASAYVTLPAEFLRPQSRGAGMGV
                     FFTVYYAGCALLPGLAGFLSDLAGSTRATLWLAALLAFICVPTLIASRYAIGAPRPAP
                     A"
     gene            13247..14044
                     /locus_tag="VFR57_03590"
     CDS             13247..14044
                     /locus_tag="VFR57_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0258489.1"
                     /translation="MTRLEKAAAWILAILWLSPLLYAFWAAFHPPEFATTFEPLAPLT
                     LGNFEKAWGYAPFPRHFLNTVMLVTMILGAQLVLCTLAGFAFARFDFAGRDIAFALVL
                     LQLMIMPEVLIVENYSTMSRLGLVDTTLGIGLPYMASAFGIFLLRQTFKTIPRELEEA
                     ARVEGCSWMGVLWRVYVPLARPTYLAYALVSVSYHWNNFLWPLVVTNSVNTRPLTVGL
                     AIFGAPESGVDWSVISAGTLIAVAPLLVAFLLFQRQFMQSFMQAGIK"
     gene            14107..14568
                     /locus_tag="VFR57_03595"
     CDS             14107..14568
                     /locus_tag="VFR57_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0258490.1"
                     /translation="MITLSINDKRVDTKAAEDTPLLWILRDHLNLTGTKFGCGMALCG
                     ACTVHVAGEPVRSCTTPISAVAGKKITTIEAIGATRVGKAVQDAWVAENVPQCGYCQS
                     GQVMAAAALLRENKKPTDAQIDEAMNGNLCRCATYPRIRAAIKRAARTLTS"
     gene            14565..16742
                     /locus_tag="VFR57_03600"
     CDS             14565..16742
                     /locus_tag="VFR57_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0258491.1"
                     /translation="MITNESRRDFLKASTALLLGVHLPQALAQSGPGKTVGAFATKPF
                     EPNAFVRVGADNTVTVLVKHLEMGQGTYTGLPTLVAEELDADWSQMRAVGAPANAKLY
                     NNLFWGEAQGTGGSTALANSYEQLRKAGASARAMLVAAAAEKWKADPKEIKVSKGVVS
                     WRDRKATFGQLAADAANQKVPENVPLKDPKDFVLIGKPARRTDARAKSNGSAQFTQDV
                     KLPGMLTAVVLHPPRFGGRLKSFDDSRAKAVPGVRRVVAFSTPVTNGVAVLAGDYWTA
                     KNGRDALTAEWDESNAVRLSSSEILDQYKKLAATPGTSARREGDADKALAGAAKVLEA
                     AYEFPYLAHAAMEPMNCVVRLGDGGCEVWNGEQFQTGDQHALAKLLGIPPEKVTLHQL
                     YAGGGFGRRANPLSDYVLEAAAIAKALDGKIPVKLVWGREDDMHAGFYRPMYYHTLKA
                     GLDEKGNIVAWKHVIVGQSILAGTAFESMMVKDGVDATSVEGANTLPYAIPNLSVELH
                     SPKTGVPVQWWRSVGSTHTAFATECFLDEIARETKKDPFELRRSLLAKHPRHKGVLEA
                     AAQKAGWGKPPAKGRVRGIAVHESFNTFVAQVVELSKQKDGLKIEKVVCAVDCGVAVN
                     PNIIAMQMESGIGYGLSAALTGAITLKEGVVEQSNFHDYPVLRMNQMPPIETVIVQST
                     EKPTGVGEPSTPVIAPALANAILAMHGKPVRALPLSAEGISFA"
     gene            complement(17017..17346)
                     /locus_tag="VFR57_03605"
     CDS             complement(17017..17346)
                     /locus_tag="VFR57_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258492.1"
                     /translation="MSAERLKPHIDALLLKLPGVSARKINGLDAYFVSDRMFACICGT
                     GVGLRLPAVDARELQFGRADVGPFQPTGLPMSKEWVQINRANAAQYEEDLPLFQASLE
                     FVKRGGR"
     gene            complement(17353..17601)
                     /locus_tag="VFR57_03610"
     CDS             complement(17353..17601)
                     /locus_tag="VFR57_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258493.1"
                     /translation="MKNSDVPMMALRRAVQICGGLDALGDALNTSTESLQRMIAGAEE
                     VPLPLFTQTVGLLLEATATRTPKRTPDSNEGGRKGPRH"
     gene            complement(17633..17836)
                     /locus_tag="VFR57_03615"
     CDS             complement(17633..17836)
                     /locus_tag="VFR57_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0258494.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGGKDLFAHHSSIQGSGFKSLKE
                     GQKVSFDVTQGQKGPAATNIKPL"
     gene            complement(17991..18455)
                     /locus_tag="VFR57_03620"
     CDS             complement(17991..18455)
                     /locus_tag="VFR57_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0258495.1"
                     /translation="MKVTKDTVVRLSVEMDGDSGGRELEYLHGGYGSLFPKLEAALAG
                     LEAGGKANVTLSPADGFGEHDPSLIRREPRERLPQGVKVGSALHGGHAEEDGHRRVFR
                     VTALSDTEATLDGNHPLAGRTVELRCEVLEVRAATQEEIEHGHVHGPHGHSH"
     gene            complement(18452..20023)
                     /locus_tag="VFR57_03625"
     CDS             complement(18452..20023)
                     /locus_tag="VFR57_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEU0258496.1"
                     /translation="MIVASGIAMQFGAKPLFENVTVKFGGGNRYGLIGANGCGKSTFM
                     QILGGELEPTAGTVAIDANERVGRLRQDQFAYENERVLDVVLMGHAEMWAAMRERDAI
                     YANPEAGEAEFLRAADLEAKYAEYGGYTSEARAGELLLGLGVPVAQHDGPMSAVAPGW
                     KLRVLLAQALFGDPDILLLDEPTNNLDINSIRWLEDVLNDRDCTMVIISHDRHFLSSV
                     CTHMADVDYGNIRVYPGSYDDYMEASTLAKTQQQNANARAKERIAELEDFVRRFRAHK
                     AKARQATSRMKMIEKLRPEEVKPSSRQYPFIRFLVDPKEKLHRIAVEAKGLFYGKLDL
                     SIEAGEKVAIIGPNGVGKTTLLQNLIRGKWAEKARVGYWPQDSAEEFAADEPLTEWMG
                     RWRRKDRGDDDQVVRATLGQLLFTGDDAKKSVKVISGGEGQRMIIGKLVLDMPNVMLL
                     DEPTNHLDMEAIESLNGALEKYSGTLVFVSHDREFVSSLATRIIELKPDGSHVDFRGS
                     YDEYLERDGAALLAA"
BASE COUNT         3388 a         6966 c         6598 g         3536 t
ORIGIN      
        1 cgaggaacac gccgaagagc gcgagcacca cgccgagcgc ggccgggaaa ttgcccggcc
       61 ccatgtgcgc gagcgtgccc atcggaaagt cgcggtcggc gatcaccatg gccgccaggc
      121 cgaacgcgag gaagacgagc ccggccagga agtccttgtt cgcgaccggg ttcaccgctg
      181 aagacggcgc tcagtatccc ggcggcaagt gatcgagctc gacgacgccg ttgcgctcgc
      241 gctcgcgcca atagtgctgg ctgccttccg gcggcacgtg cacgagctcc agcttcatgc
      301 cgtccggatc agcgaagaac accgcgtagt agcccggctc gaagtcgtag tcggccggcg
      361 catcgaggac ggtcgcgccg atcttctgga gaacgtcgtg cagcgcatcc acctgctcgc
      421 gcgtgtccgc attgagcgcg agatggtgaa agcccgttga atagcgatcg tgcttgctgg
      481 acgagccttt gcgcaggagg ctgaaaatca tccactgcag gttgccgctc ggcgtcggcg
      541 cggcgtaggc gatgcgaaac gggctgcgct caaccaggcg atagccgagg aagccgagta
      601 cgacgctgta gaagcgctcg gcgcgcggga tgtcgctgac cgtgaggcgc aggtggttca
      661 tcgaaccgag cttcgtcatc gcttcttcgc tcctgcgctg agttcggtcg cgtagccgaa
      721 aaggcccggc agtccgccgg tatgcagaaa gacaaccttc tcgccggcgc gaaagcggcc
      781 ggcgcgcgca tggcggatga gtccggccat cgatttgccg gtgtagaccg gatcgagata
      841 gagaccttcg agcgatgcgg ccatggcgag cgcctccttc acttcgggcg tcggcacgcc
      901 gtagcccggc ccgacgctct ggtcgtcgac gatcacggcg tcctccggca actcgacgcc
      961 gagcagctcc gcgagtcccg gcaagaggtc gaacaccttg ccgcgctggc gctcgagcgg
     1021 cgcgccgacg ctgaaaccga gcacgcgcgc cgcgctaccc tcgagcgcga gcccgccgag
     1081 caggccggcc tgcgtgccgc cgctcccggt cgcgagcacg atcgtgtcga agtcgtccat
     1141 ctgctcgcac agttccatcg cggcgttggc gtagccaacc gcgccgagcg gcgtcgagcc
     1201 gccgaccggc atcacatacg gacgctttcc cgccgcgcgt gatccttgtg cgacgcgctt
     1261 catgtattcg gccgtgtccg cagtattggg gatgacgtgc accgtggcgt cggcgaggcg
     1321 cgtgaggaac acattgccgc ttacggtgta ctccgggccg cggccgggca gcggctcgtt
     1381 cagcacgcct tcgcatgcga ggccgagcgc gccgcagagc gccgcggtct ggcgcacgtg
     1441 attcgactgg atcgcgccgc ctgtgaccac cacatccgcc ttctgcgcta gcgcctcggc
     1501 gagcaggaac tcgagcttgc gcaccttgtt gccgcccccg ccggcgccgg tgaggtcatc
     1561 gcgcttgacc cagatctccg gcccgccgag cgccgcgctc aggcgcttga gcggttgcag
     1621 cggcgtcggc agcgcggcaa gcgcgacgcg cggcagcgat tcaattgggt tcgccatccc
     1681 ttcactataa agctgacgaa acccaggtgc cgcctatgtc cccggctgcg gcgcgacacg
     1741 gccgatccac ttttcgaccg cctccaggat gttcgcgcgc gagtcattct tcgccacgca
     1801 gtccacgatg ccattgcctg cggctccggg cgcctcagcc tgctcatttt gcccgccgac
     1861 gagcacgatg accttcgtgt tgtgcaggcg cgcacgcaac tccgcgacga gggtgaccgg
     1921 gtcctcgccg cccggaagcc ggcggctgac cagccacgcg gcgtattccc agcggctaag
     1981 gagctcgcgg ctcgccgcgg catctgccgc ggtgtcgacg tggtagccgc ccttgtccag
     2041 caccatggcc agatactccg cggttacgga atcgtattcg gtcagaagca cgcgcaccgc
     2101 gttgtccgct gtctcggcgc cggccttcat caccggcagc gcgaggaaga aggtggtgcc
     2161 gccgccggcg cggtcctcga agccgacgcg gccctgcatc atctccacca gccgcttgga
     2221 gatggagagc ccgagcccgg tcccgccctt gatgcgtgaa tcggccgaat cggcctgtgc
     2281 gaagcggccg aaaatgcggc tcctgaattt ctccgggatc cccggcccgc gatcaccgac
     2341 ctcgacgcgc acgccgtcgc cctcgagtcg cgcgctcacc tcgacggtgc cgtccggcgg
     2401 ggagaacttg gcagcgttcg aaaggaagtt cgtcatcgct tgcatcaggc gcttacggtc
     2461 cgcgcgcacc gtcacgtcgg gcggcttgcc cacgagcgca aggtgcacct tgaaacgatc
     2521 ggcgtagccc tggttcaagc gcagcgattc ttcgagcaga cccttgagcg cgatcggctc
     2581 gggcacgagc gcaaggcgcc cggactcgat tttctcgatg tcgagcacct cgttgatcaa
     2641 atcgaggagc cgctggccgt tccgttccgc gaccgagatg agctcggcct gctcggcggc
     2701 cagctcgccg agcgctccgc tttgcaccag ctgcagcgag cccagaatcg aggtgagtgg
     2761 cgtgcgcagc tcgtggctga gcgtcgaagt gaactcgctg cgcacgcgct cggaggcgcg
     2821 ctgctgcgtg atgtcgcgcc cgtggagcac gatcgaagtc acatcgttcg cgtcgttcac
     2881 tagcggggtc acggtccatt cgcaggcgag ctcgaggccg tcgcgtcgca ggcagcgctc
     2941 cgcagtcatg gtggcgggct tgccctcctg caccatcgtg gtccaccagt gctcggtgcg
     3001 cgcgcgatca tcggcgggga ggagcatggt gaaaaggttg cgcccgacca tctcgggtgc
     3061 ggcgtaaccg aacaggttct ccgccgccgg gttgatgtcg aggatggtcg cgcgcgggtc
     3121 gagctctaag accgcgatcg gcgcgcgctc ggagaaaagt gacagcttgc gcccataggc
     3181 ctgcacctcg cgatgcgcgt cgagcaggct gtccagttgc tcggcgagtt gcgcctgcac
     3241 cgcggcgttg tcgttggaga gccgatcgta ggcgctgcgc agcccgtacg ccgtactgaa
     3301 cgctttgccg aggtgccagg cagtggcgac gtaagccgcg tacaaaagcg ggaccgccgc
     3361 gccgaaaagg cggaagaact ccgtgccgtg cgcgaacacg acgaccgcga acggcagcat
     3421 gaccgccgcg ccgtatgcgg cgtagaccca ccagaccgtc gaaaagagcg gcaagcccgc
     3481 cgccgcgacg agggcgacga cgaaggagat caacgcgtgt tcggcttggc tgcccgggaa
     3541 aaaaaccgcc gcggcgaagc cccagccggc acccagcatc ccggcggcga acgccgcgcg
     3601 gcgcaaccat gcttcgggat tctcccgacc ctgagagcgg cgataggccc agccgatcat
     3661 ccagagcgcc gcggcgagca gcaccatcac gccctgccag aacgcgacca gctcgaccgg
     3721 acgcgcgcgc cacaggaacg cgctgaagaa aaagctcaga acaagcgtcg cgacgagcgc
     3781 gatcttgagc cgactgtaaa tatagtcaca gcgctccctc agcggctccg gcaggtcggt
     3841 caacatgcgg taatcttaaa agctaaggga gtaacgcgtg aggaaaatgg tcccagtgcc
     3901 cgagcttgag ttcggcatgt acgtggccga gctcgatcgc ccgtggaccg aaacgccgtt
     3961 ccagttccag ggtttcttcc tgcagacgcg cgagcagctc gagacgctga aaaaattctg
     4021 caagacggtg cttgtcgatc tcgaccggcg cgagaccgtc gagcgcctgc ccaagccccc
     4081 tgcaggtggg gcgagcagcg ctcttgacct ctcgcgtacc gggaagaaga agtacgccga
     4141 agtcgcgccg gtcgagcacg aatacgcaag agcgagcgcg acgcacgcgg ccggcatggc
     4201 tgtggtgaac gacgcattcg ccgcgctgcg cgcgggcaag acgctcgaag caaaccgcgt
     4261 gaacgaggcc gtgaacggca tcacggaaag cgtgatgcga aatgccgacg cgctgctcct
     4321 cttcacccag ctccagagca agggcgacta cacgcaatcg cacgcgctcg acgtctcggt
     4381 gtacatgacg gcgttcggcc gtttcctcga gatgtcggcc gacgacatcc gcctgctcgg
     4441 gcacgtcggc ctgctgcagg acgtgggcaa ggttcggctg cccgacgggc tgatcgagaa
     4501 gcgcgagcgc ctcacgcggg aggagttcga gctcgcgaag agccacgtcg tgcattccgc
     4561 cgagatcctc aacgccacac ccgggctgcc accggcgctc gccgagctcg ccttcctcca
     4621 ccacgagcgc cacgatggca gcggttatcc gaaaggtcta cgcgggcgcg acatcggcct
     4681 cgtcggctcg atcgccggaa tcgttgatac cttcgatgcg ctcaccgcgc gccgcccgta
     4741 tggcgagccg gtcgcgccgt cgaccgcgct gggcatgatc tataagtggc gcggcacgct
     4801 gttcgacgcc tacgtcgtcg agcaattcat ccgattcgtc ggcatcttcc cgctcggcag
     4861 cctgatcgag ctcaactcgg gcgaggtcgg tatcgtcatc acacagaatc tcgacctgcg
     4921 cctgcagccg cgcgtcatgg tgatccgtga tgcaaacggc aacccgctga agccgcagaa
     4981 gcttgtggat ctctcgcgcg ggcacaagct cggctccggc gagctctacc gcatcaagcg
     5041 cacgctcgag tacggcagaa ttccggtgag cgccgaaagc ctcttctctt gaaatagccg
     5101 cccccgcctt cgcgggggcg acttcctagg gctttctaat gggcgccgag ccaggtgcgt
     5161 gcggcggcga cttccggccg cggctcgccc ttgccggcga gcgccacgag gcgtgtgtag
     5221 tgctgcttcg ccttcgcctg ctggcccgct tgcgaagcgg caagcccggc gccgtagtag
     5281 ccgcgcagcc ggttcggctc gcgaatgtgc gaggcttcgt actccttcag cgcgtcggcc
     5341 gggcgcttca gctctagcag catgtcgccc aggagctcgc gcgcgggaag caaccgcgcc
     5401 ggcgtcacga tatgtttctc gctcttgtct tccagatcgg cggcgcggcg catggtctcg
     5461 agcgcctcct cgttctttcc ttgcgcccgc gcgatccagg cgacggagga aagacgcatg
     5521 acctcgacct cgcccgccca gtaagcgttc ttcgcctcct tcaattgctc gccaagcgcc
     5581 gcaaggcgct cggcatcctt ttgtgcggac tccggattgc cgcttcgtgc cgcccccaaa
     5641 gctcgcgcga aatgcgtcag cgcgacggtg aacgggaact tgtcctgccg cggctccagg
     5701 cgcgcggcgg cctgccagtc ggcgcgctcc agcgccaccc gggccgacat cgccgcaacc
     5761 gcgtaggggc cgacgaagcg gggcgtgaag ttcttgaacc cgagcgcatg ctcggtgacc
     5821 ttccgcgcct cgctgtcgcg cgcgagctgc agatacgcgt agacggcata gtcgagcgca
     5881 tgagccgcct cgtcgtgctc attgttcttt tcggcggcgg acgccgagcg catgttggtc
     5941 gccgccgagt ccgcccactc gcccacgcgc gtgaagatat gcgagggcat gtgcagcgcg
     6001 tgcggcgcct cgggcgcaat cttggcgtag cggcgcgcag ccgggatgcc ctgcttcgcg
     6061 atcggcggcg cgtcgtagct gtggatgagg tagtgcgcga cccccggatg ctgcgggtac
     6121 ttcttgaact gcgcctccag aatcttcgcc gccttgaggt acgccgcgta ggtttggtcg
     6181 gtctgcagct gcgtgccggc gaggtagagc gcatagaaga tctgcgcctc gtcgtccttc
     6241 ggatacttgg cggcgagcgc ctcgtaggca ttggcgcgcg cgacctggcg ttcgcgctcg
     6301 gagcgcttgc cgaaatcctg gtagtacgcg gcgaccgcct cgagataatc gcgctcgcgc
     6361 tcggtcttcg cgcccatgcg gcgccccttg tcgatcgcgg tctgcgccgc ctcggcgtcc
     6421 ggcggcgtgg cgccctggcc ggcgagcgga ttgctcatga ggatcgaggc aaatccccag
     6481 gccgcgatgg cgcagctgct atctgcctgg gcgacctcct cgaaggtgcg ctttgccgcc
     6541 gtgtagtaga acgagtgcag catcgcgacg ccgttctgca gcttctcctg caccgccggg
     6601 ctgcaggagt tcgggaaatt gaccttgccg agaccagtca cgccttgcgc gtgtaccccg
     6661 ccggcaaaca ccagggaaac tgcgaacgaa gcaatacgaa cgtgcaccat cgaaccctcc
     6721 tccaagtgag gccgccagct taacgccgat ggcgctagtg gtgaataatc ccggcacctc
     6781 cgggtgaagc gcctacttga acctagcgcg cttccggcag ctcgatcttc acctccagca
     6841 cgtcgaggtt ctgctggcgc tcgagattca ccttgatgtc cttcgggtca atcttggtgt
     6901 atttgcagat gacgtcgatc aggtcgcgct gcagcgacga gaggtagccg gcgtggcgcc
     6961 tgcggccggt gcgctcgtgc gcgaggatga tctggagccg ctccttggct accgtggcgc
     7021 tggtttttct ttggccgagc aggaaagaga ggaatgagcc catcggtcgt tactttcctc
     7081 ccatgaggcg cttgaaaaaa ctgggtttgt agccgttcac gaagcgcagc ggtttttctt
     7141 cgccgagaaa gcgcgccacc atgtccttgt aggcctcgga gacgtccgag cctttcatgt
     7201 gcactgcggg cacgccctgg ttggacgatt gcagcaccgt ctcggactcg gggatgacgc
     7261 cgagcagctt gatccgcagg atgtcctgga tgtcatccag ggagagcatc tcgcccgagt
     7321 gcacgcgctg ggtggtgtag cgggtgatca gcaggtgctc cttgacgggc tctccgccct
     7381 ggatggcgcg cttggtcttg ctcgcgagca tgccgaggat gcgatcggag tcgcgcaccg
     7441 aggagacctc agggttggtt acgacgagcg cctcgtcggc gaagtgcatc gccatcatgg
     7501 cgccggtttc gatgccggcg ggcgagtcgc aaacgatgta ctcgtagccc atgtcggcca
     7561 gcccggtgag cactttgtcg acgccctcct tcgtgagcgc ctccttgtcg cgggtctgcg
     7621 aggcagcgag caccgacagg ttgtcgcatt gcttgtcctt gatgagcgcc tggtgaaggt
     7681 ttgcctcgcc ctggatgacg ttgaccaggt cgtacaccac gcggcgctcg cagcccatga
     7741 tgagatcgag gttccgcagg cccacgtcga agtcgatcac cgcggtcttg tgcccgcgca
     7801 gggcgaggcc gctggcaaag ctggcgctgg tggtcgtctt gcccacgccg cccttgccag
     7861 aagtcactac aacgattctt gccatttcct ctccttttcg aagaagttgt tttttccctt
     7921 cacttgagcg gctcgaacag cagccggttc ccctccgaat tcgatgccag gcgcacctgc
     7981 gtgggcttgc cccatacggc cgcgagcagc ggcgcttccc cggtccgata caggccggcg
     8041 atggagacga gctccggctc catgctcagc gcgaaaatgc gcgcatccgt gttgccgcgc
     8101 gcgcccgcga tggcgcgtcc gcgcagcggc gcgtacacgt ggatgtgccc gtcggcgatc
     8161 acttccgcgc ccgggttcac catggctagc attacgaggt cgcggccgcg cgcgtatgcc
     8221 tgctggcccg aacgcatcgg cttgtcgatc accaacgcgc cggtcggtgc ggccgcgggc
     8281 tgcgggacgg gctgcgaaac gggctgtaaa gccggctgtg aagcgggctc cggagcagct
     8341 tgtgccggca actcggcact ggaaaccccc ggcgccgcct tgccgatgcg cgcatccggc
     8401 gccggatata gaccagccgc cagcgcggcc gccgtctgct cggcgctgcc tccgtgcacc
     8461 ccgacgggca caacgcggta cttcttgagc agctcgatga gcgccgagaa gtcgaccgcc
     8521 gggcgctcgc cctccggcgc ctcggactcc aggcgcgaga gatcgatcac catggcgtcc
     8581 tggtcgaaga agtcctggat atcgccaaag cgcgcctcga gttcgtgcgc caatgcagcg
     8641 agatcgggcg acttcagcac cagggcgacc agcgggaggg ttgcgctttt aatctcgaac
     8701 gtttcgggcg tggtgccttg gacggcgagg gtcattgaac caatgccggc gccaaggctg
     8761 gcggcagcgc taccagccgg gggggctggt tactctccaa gacgcttcgt agatggacca
     8821 aacttcagcg ttctttttcg ttgttccgta cagacccacc aattgtatac ggtcaaagta
     8881 gcggccgcca atgcaccccg gcgtaatatc gccagcgtgt cggagattcg ccttgaggag
     8941 gtcaccaagc gcttcggcgc ggttagggcg gtcgacgccg tatcgttcgg cgcgcagaaa
     9001 ggaaagttcg tcgtcctgct cggtccctcg ggctgcggca agtcgacgct ccttcgccta
     9061 atcgccggcc tcgaggaagt gagcgaggga aggatcttca tcgaggatcg cgacgtcacc
     9121 aacctcgacc cgaccaagcg gcgcatctcc atggtgttcc agtcctacgc gctcttcccg
     9181 cacctcacgg tggccgagaa catcgtcttc ggtttgcgcg ttcgccgaac cgacgccgcc
     9241 gagcgcgagc gccgcctcaa caccgtggcc gagctggtcg gcctcggcga gcggctacag
     9301 cacaaacccc agcagctctc cggcggccag cgccagcgcg ttgcgctcgc gcgcgccatc
     9361 gtctcgcaga acccgatctg cctcatggac gagccgctgt ccaacctcga tgcgcagctg
     9421 cgccacgaca tgcgcgtcga aatccgcgcg ctgcagcggc ggctcggcat gacggtcgtg
     9481 tatgtgaccc acgaccaggt cgaggcgatg agcatggccg accacgtagt gctgctgcgc
     9541 gaaggtcgca tcgagcagga gggctcgccc gccgagctct acgcgcgccc cgcgaccacg
     9601 ttcgccgcgc gcttcatcgg cacgccggcg atgaatctcg tgcctttgca cgccatacag
     9661 gccggcgcga cggcgaacct taccgccggc atccggcccg aacatattcg gctgggcgac
     9721 ggcatcccgg ctacggtttt gagcgccgaa taccacggcg ccgacaccat cgtcaccgcg
     9781 caggtgaacg gctcggcgct cctcgtgcgc gcgcccggcc aggtcgacct cgccgccggc
     9841 tcgcaggtaa agcttggctg ggagcccggc gcggtgcata ttttcgattc cgaggggaga
     9901 aagacatgaa acggtttctt ggactcatcc tggccgcggc gttcgccgcc acggcctatg
     9961 cgcaaaaaac agagctgacc ttctactacc cgatcgccgt ggggggcccg ctcaccaagg
    10021 tgatcgacgg ctacgccgcc gggttcgaga aggagaaccc caacatcaag gtaaacgcgg
    10081 tctacgccgg caactacgac gatacgcgca tccgcgcgct cgccgcgcac aaggccgggc
    10141 agccggcaca gctctcggtg ctcttctcca tcgatctcta tgaactcctc gagcaggacc
    10201 tgatcgtgcc gtttgacgac atggcgaaga ccccggaaga caaggcgtgg ctgaagagct
    10261 tctaccccgc gctgatgacg aacggcacct acaagggcaa gacctacggc atcccgtttc
    10321 agcgctcgac catcgtgctc tactggaaca aggccgcgtt caaggaggca ggcctcgatc
    10381 cggagaagcc gccgacgaac tggaacgaga tgcgcgacat ggcggcgaag ctcgtgaaga
    10441 aggacggcgg caacgtcacg cgctggggcg tgatggtgcc ctccaccggc tacccatatt
    10501 ggatgttcca ggccttctcg cgccagaacg gccaggacct catgaacccg gagggcaacc
    10561 gcaccaactt cactcacccg gacgtcgtcg cggccctcca gtactgggtg gatctgggaa
    10621 gaaaagaggg agtcatgccc gccggcacgg tcgagtgggg gacgctgcgc cagaacttcc
    10681 tcgagggcaa gaccgcgatg atgtggcaca ccaccggcaa cctgactgcg gtgaaagacg
    10741 gcgccaagtt ccccttcggc gtcgccatgc tgcccgcctc caagcagcgc ggctctccca
    10801 ccggcggcgg caactggtac ctcttcaaga agtccacgcc gcaggagcgc gaggccgcgt
    10861 tcaagttcat caaatggatc accgcgcccg agcgcgcggc cgactggtcg atcgccaccg
    10921 gctacgtcgc ggtgagtccg gccgcctacg agacaccgaa gctcaagagc tacgtctcgg
    10981 agttcccgcc cgccgcggtg gcgcgggatc aattcaagtt cgccacgccc gagctctcca
    11041 ccttccagac cggccgcgtg cgcaagagcc tggacgacgc catccaggcc gcgctcaccg
    11101 gctcgaagag ccccgccgac gcgctcaaga ccgcgcaaac cgaatcggag aggctgctca
    11161 aaccgtacaa gtaagaaaat ggggacagac cccatttttg gatgaagcat gtctacggat
    11221 ggctgctgct caccccggca gccatcctgc tcgtcgcctt cacgcactac cccgcggtcg
    11281 cgacgctcgc cgacagcttc ttctcgcacg ccaccgcgct ccgccccgcg cgcttcgtcg
    11341 gcgtcgacaa ctacgccgcg ctcgcgggcg acccggtctt ctggcaggtt cttaagaaca
    11401 atttctggtt cgcgctcggc acgatcccca cctcgatcgc gctcgcgctc gtcatggcgc
    11461 tctgggtgaa cgcccgcctc cccggccgcg cgctcgtgcg cctcgcgtac ttcacgccga
    11521 ccgtgctgcc gatgatcgcg gtggcgaacc tgtggctcta cttttacacg ccgcagatcg
    11581 ggctcctcga ccaggtgacc gggctcttcg gcgcgccgtc gcacaactgg ctcggcaacc
    11641 ccgacaccgt gatgggctgc gtgatcgcca tgacgatctg gaaggaagcg ggctttttca
    11701 tgatcttcta cctcgccgcg ctgcaaacgc tcccgcccga gctcgaggag gcggcgcgca
    11761 tcgagggcgc ctcgcgcggc tacttcttct ggcgcgtcac cttcccgctg ttgatgccca
    11821 ccacgctctt cgtgctggtg accgcgacca tcaactcctt caagctcgtc gaccacctct
    11881 tcatcttgac gaagggcggc ccggacaacg cctcgaacct gctcctctat tacatctacc
    11941 aggtggcgtt cagctatctc gacaccgcgt atgccgcgac gcttaccgtg atcctcctct
    12001 ttctcctcgc cgcgttcgcc gtcctgcagt ggcagatgat cgagcgccgc gtgcactacc
    12061 gctagcgcat ggccattgcg caacgctggc gcatcctggg cgccctgacc ttcgctcgca
    12121 cgagcatcgg cttccagttc caatccgtcg cgccgcttgc gccccttgtc agcggcgagc
    12181 tcggactcga caacaccgag ctcggctggc tgatcggcct gtacctgctt cccggcgtgg
    12241 tggccgcact gcccggcggc ctgctgggcg cacgttacgg cgacaagcgc atgaccctgc
    12301 tcggcttggc gctcatggcc ggcggcggaa tttggctcgc aagcgcggcg agcttcgccg
    12361 aggctgatgc tgcgcgcctg gtgagcggca tcggtgccgt ggtcctcaac gtgcttctca
    12421 cgaagatggt cgcggactgg ttcgagggca aggagcggct gctcgccatg tcggtcctaa
    12481 tcaacgcgtg gccgatcggc atcggccttg cgcttcttgc gctgcccctg atcggcgagg
    12541 cgaccggctg gcgtggcgcc atcgccgccg cggccctgct tgcggccgcc ggcttcacag
    12601 cggtgctttt cttctatcgt gcgccagcag cagcgcaggc ggggcccgac ggcggcgtcg
    12661 gactgaacgc cctcgacgcc aaggagtggc gcctgctcgc cgtcgcctca atcccatggc
    12721 tcgccttcaa cgcggcctac cagctcgtcg tgtcgtttct gccatcgttt ctgctcgaga
    12781 ccggttcgag cctgaccttc tccggggcgt tgaccgcgct gaacaccgcg ctcgtcatcg
    12841 tctccgtgca ggcgggcggc gtgctgctca agcgctcgcg ccgccctgac ctgctctgcc
    12901 atgtggcgat cgtcgtctgg tgcgcgagcc tgctcctgct tgcgagcgaa tccgcacccc
    12961 tgctctggat cgtcgccgga gggctcatcg ccggaatccc ggcaagcgcc tacgtcaccc
    13021 tgccggccga attcctgcgg ccgcaaagcc ggggggccgg catgggcgtg tttttcaccg
    13081 tctactacgc gggctgcgcg cttctgccgg gactcgcggg attcctgtcc gacctcgccg
    13141 gcagcacccg agcgacgctc tggcttgccg ccctgctcgc gttcatctgc gtgcctacgc
    13201 tcatcgcttc gcggtatgcg atcggcgctc ctcggccggc gccggcatga cgagactcga
    13261 aaaagccgcc gcctggatcc tcgcgatcct gtggctctcg ccgctcctct acgccttctg
    13321 ggcggcgttc cacccgccgg agttcgcgac gacattcgag ccgctggcgc cgctcacgct
    13381 cggcaacttc gagaaagcct ggggctacgc gcccttcccg cggcatttcc tcaacaccgt
    13441 gatgctggtg accatgatcc tgggcgccca gctcgtgctc tgcacgctcg ccggcttcgc
    13501 cttcgcacgc ttcgacttcg ccggccgcga catcgccttc gcgctcgtgc tgctgcagct
    13561 catgatcatg cccgaggtgc tgatcgtcga gaactacagc accatgagca ggctcggcct
    13621 ggtcgacacc accctcggta tcggccttcc ctacatggcg agcgcgttcg ggatcttcct
    13681 cctgcgacag acattcaaga ccatcccgcg cgagctcgag gaagccgctc gcgtcgaggg
    13741 ctgctcgtgg atgggcgtgc tgtggcgtgt ctacgtcccg ctagctcggc cgacgtatct
    13801 cgcgtatgcg ctggtctcgg tgagctacca ctggaacaac ttcctatggc cgttggtggt
    13861 gactaactcg gtgaacacac gaccgctcac cgtcggcctg gcgatctttg gtgcaccgga
    13921 atcgggcgtc gactggtcgg tgatcagcgc cggcaccctg attgcggtcg cgccgctgct
    13981 cgtcgccttc ctgctcttcc agcgccagtt catgcagtcg ttcatgcagg cgggaatcaa
    14041 gtaaggtatt acggctgcca cgctgcggcg ttcgaatgtg caccatggag ttgaacggag
    14101 gcctgaatga tcaccctcag catcaacgat aagcgcgtgg acaccaaggc ggccgaggac
    14161 acgccgcttc tctggatcct gcgagaccac ctcaacctga ccggcaccaa gttcggctgc
    14221 ggcatggctt tgtgcggcgc gtgcacggtg cacgtcgccg gggagccggt gcgaagctgc
    14281 accacgccga tctccgccgt ggccggcaag aagatcacca ctatcgaggc gatcggcgcc
    14341 acgcgcgtcg gcaaagcagt gcaggacgcc tgggtggcgg agaacgtgcc gcaatgcggc
    14401 tactgccagt cgggccaggt gatggccgcc gcggcgctgc tgcgcgagaa caagaagccc
    14461 accgacgccc agatcgacga ggcgatgaac ggcaacctct gccgctgcgc cacctacccg
    14521 cgcatcaggg cggcgatcaa gcgcgccgca aggaccctta cgtcatgatc accaatgaaa
    14581 gccgcaggga tttcctcaaa gcctccaccg cgctgctgct cggcgtgcac ctcccgcaag
    14641 cgcttgccca gtcggggccg ggtaagaccg taggcgcttt cgcgaccaag ccgttcgagc
    14701 cgaatgcgtt cgtgcgcgtc ggcgccgaca acaccgtgac cgtgctggtg aagcacctcg
    14761 agatgggcca gggcacctac actggcctgc cgacgctagt cgccgaggag cttgacgcgg
    14821 actggtcgca gatgcgcgcg gtcggcgcgc ctgccaacgc aaagctctac aacaatctct
    14881 tctggggcga ggcgcagggc accggcggca gcaccgcgct cgcgaactcc tacgagcagc
    14941 tgcgcaaggc cggcgcttcg gcgcgcgcca tgctggtcgc cgcggccgcc gaaaaatgga
    15001 aggccgatcc caaggaaatc aaggtttcca agggcgtggt gtcgtggcgg gaccgcaagg
    15061 ccacgttcgg tcagctcgcc gcggatgccg cgaaccagaa agtcccggag aacgtcccgc
    15121 tgaaggaccc gaaagacttc gtcctgatcg gcaagcccgc gcgccgcacc gacgcgcgcg
    15181 cgaaatcgaa cggcagcgct cagttcacgc aggacgtaaa gctccccggc atgctaacgg
    15241 cggtcgtgct acatccgccg cggttcgggg ggaggctcaa gagcttcgac gactccaggg
    15301 ccaaggccgt gcccggcgtg cggcgcgtgg tcgcgttttc cacgccggtc accaacggcg
    15361 tcgccgtgct ggccggcgac tactggacgg cgaagaacgg ccgcgacgcg ctcacggccg
    15421 agtgggacga gagcaacgcg gttcgtctat cttcttcgga aatactcgac caatacaaga
    15481 agctcgccgc gacccccggc acctccgcgc gcagagaagg cgacgccgac aaggcgctcg
    15541 ccggcgcggc gaaggtgctg gaggccgcgt acgaattccc gtacctcgcg cacgccgcga
    15601 tggagccgat gaactgcgtg gtcaggctcg gcgacggcgg ctgcgaggtg tggaacggtg
    15661 agcagttcca gaccggcgac cagcatgcgc tcgccaagct gctcggcatc ccgcccgaga
    15721 aagtgaccct ccaccagctc tacgccggcg gcggtttcgg ccgccgcgct aacccgctct
    15781 ccgattacgt gctcgaggcc gcggcgatcg cgaaagccct cgacggcaag atccccgtca
    15841 agctggtctg gggccgcgag gacgatatgc acgccggctt ctaccggccg atgtactacc
    15901 acacgctgaa agccgggctc gacgagaagg gcaacatcgt cgcctggaag cacgtcatcg
    15961 ttggccagtc gatcctcgcc ggcaccgcct tcgagtccat gatggtgaag gacggcgtcg
    16021 acgccacctc cgtcgagggc gcgaacacct tgccctacgc catccccaac ctgtcggtcg
    16081 agctgcactc cccgaagacc ggcgtccccg tgcagtggtg gcgctcggtc ggctcgaccc
    16141 acaccgcctt cgccaccgag tgcttcctcg acgagatcgc gcgcgagacg aagaaagacc
    16201 cctttgagct gcgccgctcg ctcctcgcga agcacccgcg gcacaagggc gtgctcgagg
    16261 cggccgctca gaaagccggc tggggcaagc cgcccgccaa gggccgcgtg cgcggcatcg
    16321 ccgtgcacga gtccttcaac actttcgtcg cccaggtggt cgagctctct aagcagaagg
    16381 acgggctgaa aatcgagaag gtggtctgcg ccgtcgactg cggcgtcgcg gtcaacccga
    16441 acatcatcgc catgcagatg gagtccggta tcggctacgg cctctccgcc gccctcaccg
    16501 gcgccatcac gctgaaggaa ggcgtggtcg agcagtcgaa cttccatgac tacccggtgc
    16561 tgcggatgaa ccagatgccg ccgatcgaga ccgtgatcgt gcagtcgacc gagaagccga
    16621 ccggggttgg tgaaccgtct acgccggtga ttgctccggc cttggcgaac gcgatcctgg
    16681 cgatgcatgg gaagccggtg cgggcgctgc cactttcggc cgagggaatc tcgttcgcat
    16741 agcttgccag gttgcctgca acgttccccc gttagtggcg tcaatggcca gctgatcagc
    16801 agtaggcgac gacgttgccg tcaacgacga ttcgttcttc tcgtcgcttt cgcgcaggcg
    16861 aaaatgcaga tccttgggga tgttctttag cgtaaggctg cgcatcggtg cctccttgga
    16921 accaaaatgg aaccgatctg atgcgcagtc agcgccaacg ctaaatcgca ggtcaagtta
    16981 tgggtcaagc cactgcgagc gtgacgcggc gccgggctag cgaccgccgc gcttcacgaa
    17041 ctccagcgaa gcctggaaga gcggcagatc ctcttcgtat tgcgccgcgt tagcgcggtt
    17101 gatctgcacc cactccttcg acatcggcaa gccagtgggc tgaaaagggc cgacgtcggc
    17161 gcggccgaac tggagctccc gtgcgtcgac cgccggcagg cgcagtccca cgccggtgcc
    17221 gcagatgcag gcgaacatgc ggtcgctgac aaagtaggcg tcaaggccgt tgatcttgcg
    17281 cgcgcttacg cccggaagct tgaggagcag cgcgtcgatg tgcggcttca ggcgctctgc
    17341 actcatgcag ctctaatgac gcggcccttt gcggccgccc tcgttagagt ccggcgttcg
    17401 ctttggcgtg cgcgttgccg tcgcttccag gagcaggccc acggtctgcg tgaaaagcgg
    17461 cagcggcact tcttctgccc ccgcgatcat gcgctggagc gactcggtcg acgtattaag
    17521 cgcgtcgccg agcgcgtcca ggccgccgca gatctgtacg gcgcggcgca gcgccatcat
    17581 cggaacatcg gaattcttca tacccgatga tagcggcccc gcggccgcac gttcaaagcg
    17641 gcttgatgtt cgtcgccgcc gggcccttct gaccttgcgt gacgtcgaag gaaaccttct
    17701 ggccctcctt cagcgacttg aagccgctgc cctggatcga gctgtgatgc gcgaacagat
    17761 ccttgccgcc gtcgtccggc gtgatgaatc cgaagccctt ggaatcgttg aaccacttca
    17821 cggtgccggt tgccatgtca ctccatcctt gcggttgcac gaggctgtcc gagccccgct
    17881 tgcgggcatg atgatcaaga gaagggaatt gactgccggt aggagtattc gcttgcttga
    17941 gatcctgcgg accgtatcgt agcagcctac gaccggcgtc aagcaggaaa ctaatgcgaa
    18001 tggccgtgcg gcccgtgcac gtgaccgtgc tcgatctcct cctgcgtggc agcgcgcact
    18061 tccagcacct cacagcgcag ctcgacggtg cgccccgcga gggggtgatt gccgtccagc
    18121 gtcgcctcgg tgtcgctgag cgcggtcacg cggaacaccc gccggtgccc gtcttcctcg
    18181 gcgtggccgc cgtgcagcgc cgagccgacc ttcacaccct gcggcagccg ctcgcgcggc
    18241 tcgcggcgga tcagcgacgg atcgtgctcg ccgaagccgt cggccggcga gagcgtgaca
    18301 ttcgccttgc cgcccgcctc gagccccgcc agcgccgcct cgagcttcgg gaacaggctg
    18361 ccgtagccgc cgtgcaggta ctccaactcc cgcccgccgc tgtcgccatc catctcgacg
    18421 ctcaggcgga ccacggtatc tttcgtgacc ttcatgccgc gagcagcgcc gcgccgtcgc
    18481 gttccaggta ctcgtcgtag ctcccgcgga agtcgacatg gcttccatcg ggcttgagct
    18541 cgatgatccg cgtggcgagg gacgacacga actcgcggtc gtgcgagacg aaaacgaggg
    18601 tgcctgagta tttctcgagc gcgccgttca gcgactcgat cgcttccatg tcgaggtggt
    18661 tggtcggctc atccagcagc atcacgttcg gcatgtcgag cacgagcttg ccgatgatca
    18721 tgcgctggcc ttcgccgccg gagatgacct tcaccgactt cttcgcatcg tctccggtga
    18781 agaggagctg cccgagcgtc gcgcgcacga cctggtcgtc gtcgcctcta tccttgcgcc
    18841 gccagcgccc catccattcg gtgagcggct cgtcggcggc gaactcctcc gccgagtcct
    18901 gcggccagta gccgacccgc gccttctcgg cccatttgcc cctgatcagg ttctgcagca
    18961 gcgtggtctt gccgacgccg ttggggccga tgatggcgac cttctcgccc gcctcgatcg
    19021 agagatcgag ctttccgtag aaaaggccct ttgcctccac cgcgatgcgg tgcagcttct
    19081 ccttgggatc gaccaggaag cggatgaacg ggtactggcg gctcgagggc ttgacctcct
    19141 cgggcctcaa cttctcgatc atcttcatgc gcgaggtggc ctggcgcgcc ttcgccttgt
    19201 gcgcgcggaa gcggcgcacg aagtcctcga gctcggcgat gcgctccttg gcgcgcgcgt
    19261 tggcgttctg ctgctgggtc ttcgcgagcg tcgacgcctc catgtaatcg tcgtagctcc
    19321 cggggtagac gcggatgttg ccgtagtcga cgtccgccat gtgcgtgcag acgctcgaga
    19381 ggaagtggcg gtcgtgcgag atgatcacca tcgtgcagtc gcggtcgttg agcacgtcct
    19441 ccagccagcg gatgctgttg atgtcgaggt tgttggtcgg ctcgtcgagc agcaggatgt
    19501 ccgggtcgcc gaacagcgcc tgtgcgagca gcacgcgcag cttccagccc ggcgccaccg
    19561 cgctcatcgg gccgtcgtgc tgcgcgaccg gcacgcccag gccgagcagc agctcgcccg
    19621 cgcgcgcctc cgaggtgtag ccgccgtact ctgcgtactt ggcctccaga tccgccgcgc
    19681 gcaggaattc ggcttcgccc gcctccgggt tggcgtagat cgcgtcgcgc tcgcgcatcg
    19741 cagcccacat ctcggcgtgg cccatcagca ccacgtcgag cacccgctcg ttctcgtagg
    19801 cgaactggtc ctggcggagc ctgccgacgc gctcgttggc gtcgatcgcc accgtgcccg
    19861 cggtgggctc gagctcgccg ccgaggatct gcatgaaggt cgacttgccg cagccgttgg
    19921 cgccgatcag gccgtagcgg ttgccgccgc cgaacttgac ggtgacgttc tcgaacaggg
    19981 gcttggcccc gaactgcatg gcgatgccgg aggcgacgat catgggatac ccgggaaaaa
    20041 gtcggcagaa aataaaaaag ccggccgcag ccagctttgg tgaaagcaca ggaacgaggg
    20101 cggattatac cgtgggcccc cgccttcgcg ggggcgacga gcccctacga gggagttagg
    20161 ttttggctgg aatcagcagc tcggcgcatt cctgcgcgac gccggggcgg ggctccttgg
    20221 cgtcagtcgg gcttcaagtc ggagagctca tggcgcgtgc tggcggggcg tcaggttcca
    20281 ggaccatccc caattcgtgt tattgaccgg acggtaccgt cctgagaacc tgcggctcta
    20341 ggggaatact gccatggcac acgcgagagc cggtcaggct gcgatccatg aagcgcgcgt
    20401 tgcggtgaga gcagcgcctc cggacgagac ctccaagccg catcctttcg cacttcagta
    20461 cctggggctg cgaacggccg tgggcatc
//
LOCUS       DASTPD010000102        11790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_460077, whole genome shotgun sequence.
ACCESSION   DASTPD010000102 DASTPD010000000
VERSION     DASTPD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460077"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFR57_03630"
     CDS             <1..270
                     /locus_tag="VFR57_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258497.1"
                     /translation="TASYTVSSTDLNGSVSVTTPNAILIMPGVSTYPHDGLIRVTGAN
                     NSRADISIHSSTPTDPSAVQVEIDANGDSVFETTRLFSWNDLEGI"
     gene            complement(322..621)
                     /locus_tag="VFR57_03635"
     CDS             complement(322..621)
                     /locus_tag="VFR57_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU0258498.1"
                     /translation="MIDVRAWDRFVHAKPGRRFQDRYRRMREDKGSTWKRCVVFCGGI
                     LLVLVGLFFMAVPGPGIPIFAVGLALLAQESAALARALDRTEIKLRRLWKRFRQR"
     gene            708..2834
                     /gene="glgX"
                     /locus_tag="VFR57_03640"
     CDS             708..2834
                     /gene="glgX"
                     /locus_tag="VFR57_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU0258499.1"
                     /translation="MNAPELRLSRVKEGLPYPRGATWDGKGTNFALFSGNATRVEVCV
                     FDKSGKETERIELPEYQNEVWHGYVPDIRPGTPYGFRVHGPYAPDEGHRFNPNKLVLD
                     PYARAHIDELKWDPAIFGYTVGAENADLSFDPRDSAPYMPKCVVVDPDFDWNREKHWR
                     KVPWDRTILYETHVKGYTKLHPKVPEHQRGTYCGLATKDVIDYIKALGVTSIELLPVH
                     TFINDKFLLDKGLTNYWGYNSIGFFAPDPRYAADPSKTLQEFKEMVARFHDAGLEVII
                     DVVYNHTAEGNELGPTLSFKGIDNVSYYRLMPDRKRYYINDTGTGNTLNLSHPVVIQM
                     VTDSLRYWVEEMHVDGFRFDLGTILAREPGGFDTHNGFMKAVAQDPMLAKVKLIAEPW
                     DCGPGGYQVGGFPPGWAEWNDKFRDTVRDYWRGEASPAELAPRLCGSPDVFARRGRQP
                     WSSVNFITAHDGFTLYDLVSFNDKHNEANGEGNRDGHSHNRSWNHGFEGSTDDEAVRG
                     LRKRQMKNMLATLLLSQGTPMILAGDEFGRSQRGNNNAYCQDNEISWVRWVHDDRAQR
                     LIRFVKKLIGLRSRYPILRRTRWLTGQYDEQLGVKDVTWINATGDEMREEHWKDANVR
                     CFGMLLDGRAQSTGIRKRGEEATLLIIFNPWQDVVKFKLPGANGGEGWKLLADTNFPD
                     SPDEPDFEPGHEYEVTARSLLLFQLT"
     gene            2845..4635
                     /locus_tag="VFR57_03645"
     CDS             2845..4635
                     /locus_tag="VFR57_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0258500.1"
                     /translation="MTAAAFQTRGARPGFSTVLALVVVAAILHLARDVLVPLALAILL
                     SFLLAPGVSFLERCRLGRVPATAVIVILSFSIIGAVGWFAASQAVSLAAKLPEYRENI
                     SAKIRALRAPSDGKLGQAAEAIRELEDEAAPEQPPLAVKETAATPLAALIEWTTPFAK
                     PLGLALAVIIFTILLLLNREDMRDRLIGLIGAGRINLTTQAFGEASGRVSRYLFMQLV
                     VNAGFGIPFGIALYIIGIPNAMLWGLLAILLRFIPYAGIWIAIAMPVTLAFAISDGWS
                     LVAWTVGVFAALELIFVYGVEPWLYGRSTGLSPIAIIAAVIFWTWLWGPVGLLLATPL
                     TVCVAVMGRYIPELGFLNVLLGVEPVLTPEARLYQRLIALDSDEAIDVAEEYANEHGL
                     LALHEAVVIPALALAEHDRHRGVIDGPRERFVFDTVRKIIEDVEDRKAMKEDTEHTSE
                     DGEAKPAVPAVAAAPPSRAPTALCIAPAHDEADHLAGLAFARTLPPADFDVQVIGYPV
                     LAGEIVEQIAKSGAKIACISAVPPQAVVQASYIVKRLKSRVPGVKTVVVIWTKEDIAR
                     ARSRLGDAGADKIVSNIPDAIAQLHELSVQ"
     gene            4632..5912
                     /locus_tag="VFR57_03650"
     CDS             4632..5912
                     /locus_tag="VFR57_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0258501.1"
                     /translation="MKASKLPLAVLATAGVALAATVVAANFSHREKRIGQRIKRLYSA
                     ADPDFARAMGVLLGPAIVDGNRFAVLLNGDQIFPAMLAAIRQARKSITFESYIYWSGT
                     VGSEFAEALAERSRAGVKVHVLLDWIGSNKMDPAQLARMEQAGIEVRRFHAPHWYNLA
                     RVNNRTHRKVLVVDGEIGFTGGVGIADQWTGAAQDAEHWRDTHFRAEGPVVAQMQAVF
                     LDNWIKATGVVLHGADYFPELKPRGTGSAQMFSSSRSGGSESMQLMYLLAITAAQRSI
                     HLSTAYFVPDSLTLRTLADAVKRGVKVQIITPGEHTDAEIVQHASRARWGALLEAGAE
                     IYEYVPTMYHCKVMIVDALLVSVGSTNFDNRSFQLNDEANLNIYDAEFAARQVEIFED
                     DLRQSRRISLAEWRQRPRVEKLLDRTSSLFGSQM"
     gene            complement(5993..6205)
                     /locus_tag="VFR57_03655"
     CDS             complement(5993..6205)
                     /locus_tag="VFR57_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258502.1"
                     /translation="MKTLLLLGAGALAMYLMDRELGPQRRAQLREQLGRAKRRLSERA
                     HGGAVRTERDAEHASQVYPGAGPIGR"
     gene            6284..6730
                     /gene="aac(6')"
                     /locus_tag="VFR57_03660"
     CDS             6284..6730
                     /gene="aac(6')"
                     /locus_tag="VFR57_03660"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HEU0258503.1"
                     /translation="MFIEACESAAHPGWLRLREALWPDCARAKHLAEMNSFVAQPERY
                     VQFLAYSPTHEAIGFAELSLRSDYVNGTESSPVAFLEGLYVVPQARRKGVARALVAVA
                     SEWGRRAGCGELASDAVLENSISHLVHRALGFEETERVVYFRRKLA"
     gene            6727..7074
                     /locus_tag="VFR57_03665"
     CDS             6727..7074
                     /locus_tag="VFR57_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0258504.1"
                     /translation="MKYQGGCHCGGIKFEVEGELKGVMACNCSICTRKGALLWFIPRE
                     NFHWLTPEKAANAYTFNKHRIKHRFCPNCGIHTHGEASDPSGKPMAAINTRCLEGIDL
                     AKLPVKHFDGRSL"
     gene            7071..7478
                     /locus_tag="VFR57_03670"
     CDS             7071..7478
                     /locus_tag="VFR57_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0258505.1"
                     /translation="MRTGGCLCGAVRYQTPLKAARTVACHCKFCQRCTGSAFAVWPTF
                     ARGEVELSGALTSYEHRSDESGRWIRLDFCPRCGTTVTSTFEKGPAEIAILAGTFDAT
                     DWITVDRHVWTRSKHDWISIPAGVPAYERSSTA"
     gene            7475..7798
                     /locus_tag="VFR57_03675"
     CDS             7475..7798
                     /locus_tag="VFR57_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258506.1"
                     /translation="MIRYALCLVATNALAQPCENPVIPKAKDPAALVQAEHQWLRDAY
                     GGGTLVRQVLGASSDGRRRYDVITWRKPDGETVEVCFDVTTVFVETIREVEEEEAGAA
                     RRPAR"
     gene            7773..8156
                     /locus_tag="VFR57_03680"
     CDS             7773..8156
                     /locus_tag="VFR57_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU0258507.1"
                     /translation="MLRVVRLGSPRLAGEGLRIGVARRPPRGVKKSDYARRDFYDVWL
                     PELAPSQKQVSWALAQPWTDGRWRRYEKAYRAEMKAPAAQRLIELLARLSQHADFSVG
                     CYCEREDRCHRSILKKLLAQRGARL"
     gene            8140..8655
                     /locus_tag="VFR57_03685"
     CDS             8140..8655
                     /locus_tag="VFR57_03685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258508.1"
                     /translation="MAHGSEAPSSWVVRWAALVKHGPVLDVASGAGRHARFFAERNLA
                     VVAVDRQRQSIPGVKFVQADLEDGSPWPFPGERFGAIVVTNYLHRPLLPMIGASLGAG
                     GVLIYETFMIGNERYGKPSNPNFLLRPGELLQSFAELTVLGFEQGTTGGAVVQRLCAI
                     RGEASSARIPA"
     gene            8652..9560
                     /gene="dapA"
                     /locus_tag="VFR57_03690"
     CDS             8652..9560
                     /gene="dapA"
                     /locus_tag="VFR57_03690"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU0258509.1"
                     /translation="MITGSLVAIITPMHEDGRLDLARFRDLIDWHVAEGTDGIVVVGT
                     TGESPTVNFDEHKELIRIAVQHSRGRIPIVAGTGGNSTAEAVELTESAKKAGATACLS
                     VVPYYNKPTQEGLYRHFRKIAEAVDLPMILYNVPGRTVADLQTDTVLRLAQVPGIIGI
                     KEATANIERNTDLIRRAPRDFAIYSGDDSTCLALILMGGHGVISVTANVAPKRMHQMC
                     AAALVGDVKKARELNFKLFPLHQKLFVEANPIPVKWALAEMGMIEPGLRLPLSPLAEK
                     FHETVREALQEAGIALPGLRVVQGRG"
     gene            9560..10711
                     /gene="bamC"
                     /locus_tag="VFR57_03695"
     CDS             9560..10711
                     /gene="bamC"
                     /locus_tag="VFR57_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEU0258510.1"
                     /translation="MRKALLATAVAAAVLPGCSWFSDSSSVDYKSAGKVPPLEVPPDL
                     TSPQRDNRYAIPEQAPRSSATLSGYEAERREPAKNPGAATPGAAAQPVLLPQVERIKV
                     ERAGTQRWLVVQEPPEKLWPLVKEFWTDNGFAIRMELPEAGVMETDWAESRARIDEGA
                     MRGLLSRALDYFHSTSERDKYRTRLDRTPDGASEIYISHRGMQEVYTTARDPTSSAPG
                     ATAWQPRPSDPELEAEFLRRLMVKLGTPGERAKQLTASAEPQMRAEIVKSNDGTERLQ
                     VYEAFDRAWRRVGLALDRVGFTVEDRDRQKGLYFVRYADPESEAAASDPGFLGRIFGS
                     GSKVKAEQYRVQVAQQANGSQVLVLNKDGGAERSKTAQRILTLLHEQLK"
     gene            10715..11485
                     /locus_tag="VFR57_03700"
     CDS             10715..11485
                     /locus_tag="VFR57_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0258511.1"
                     /translation="MRFASLASGSSGNCLVAEADGTVLLVDCGLALTETERRLQRLGL
                     EPSQVSGILITHEHGDHACGAFDFAAAHRVTVYLTHGTAAALKAEGKVLDGVPLCIVN
                     GRQAFFIDGLQLVPFTVPHDAREPIQCVVSDGAAKLGVLTDVGMSTPHVERMLGGLDA
                     LVLECNYDADMLWAGGYPRWLKERIAGPFGHLDNRESERILAAIDRSKLKHIIGAHLS
                     QANNRPELARAALARALGCAESWISLATQADGFGWRQL"
     gene            complement(11488..11622)
                     /locus_tag="VFR57_03705"
     CDS             complement(11488..11622)
                     /locus_tag="VFR57_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258512.1"
                     /translation="MKLLLPAIALAIFLAACGDDASKKGTTGASTPPPAEKKTDPMKK
                     "
BASE COUNT         2077 a         3878 c         3863 g         1972 t
ORIGIN      
        1 acggcgagct acaccgtttc gagcaccgac ctgaacggtt cggtctcggt gacgacgcca
       61 aacgcgattc tcatcatgcc aggggtttcg acgtacccgc atgacggtct tatccgagtg
      121 accggtgcga acaatagtcg ggcggatatc tccatccaca gcagcacgcc gaccgacccg
      181 agtgccgtgc aggtcgagat cgatgccaat ggcgatagcg tcttcgaaac gacgcggctc
      241 ttttcgtgga acgacttgga agggatctaa gctagctcta tcgccaagaa agggcgcggc
      301 gggagccgcg cccttttgct tctaacgctg ccggaaccgt ttccagagcc ggcggagctt
      361 gatctcggtc cggtcgaggg cccgggcgag ggcggcggat tcctgcgcga ggagggcgag
      421 gccgacggcg aagatcggga tgccggggcc ggggacggcc atgaagaaaa gccccaccag
      481 gacgagaagg atgccgccgc agaagacgac gcagcgcttc cacgtgctgc ccttgtcttc
      541 gcgcatgcgc cggtagcgat cctggaaccg ccggccgggc ttggcgtgaa cgaaacggtc
      601 ccaggctcgc acgtcgatca tggctttcgt gatgcaaggg gtgggccaaa tgatccggtt
      661 ggcatatgcg cggcacgacg cttgcgttct attgtctgct cccacacatg aacgctcccg
      721 aactgcggct ttcccgcgtc aaggaaggcc tgccttatcc ccgcggcgcc acgtgggacg
      781 gaaaagggac caacttcgcg cttttctcgg gcaacgcgac ccgggtcgag gtttgcgtat
      841 tcgacaagag cgggaaggag acggagcgca tcgagttgcc ggagtaccag aacgaggtct
      901 ggcacggcta cgtgcccgac atccgtccgg gaactcctta cggcttccgg gtacacggtc
      961 cctacgctcc ggacgagggg caccgcttca acccgaacaa gctcgtgctc gatccgtatg
     1021 ctcgggcaca catcgacgag ctgaaatggg acccggcgat tttcggttac acggtcggtg
     1081 ccgaaaatgc cgacctctcg ttcgacccgc gcgacagcgc gccgtacatg ccgaagtgcg
     1141 tcgtggtcga cccggacttc gactggaacc gagagaagca ttggcgcaag gtgccgtggg
     1201 accgcacgat cctttacgag acgcatgtca agggctacac caagctccat cccaaggtgc
     1261 cggagcatca gcgcgggacc tactgcggcc tcgcgacgaa ggacgtaatc gactacatca
     1321 aggcgctcgg cgttacctcc attgagctcc tgcccgtcca caccttcatc aacgacaagt
     1381 tcctgctcga caagggcctc accaactact ggggctacaa ctcgatcggg ttcttcgcgc
     1441 ccgacccgcg ctacgccgcc gacccgtcga agactctgca ggaattcaag gaaatggtgg
     1501 cgcgcttcca cgacgcagga ctggaagtca tcatcgacgt ggtctacaac cacacggccg
     1561 aaggtaatga gctcgggccc acgctctcct tcaagggcat cgacaacgtc tcgtactacc
     1621 ggctgatgcc ggaccgcaag cgctactaca tcaacgacac cgggaccggt aacacgctca
     1681 acctttcgca tccggtggtc atccagatgg tcaccgacag cctgcgttac tgggtcgagg
     1741 aaatgcacgt ggacggcttc cgcttcgacc tgggcacgat cctcgcgcgc gagccgggcg
     1801 gcttcgacac gcacaacggc ttcatgaagg cggtcgcgca ggatcccatg ctcgcaaaag
     1861 tgaaactgat cgccgagccc tgggactgcg gcccgggcgg ctaccaggtg ggaggtttcc
     1921 cgccggggtg ggccgagtgg aacgacaagt tccgcgacac ggtgcgcgat tactggcgcg
     1981 gcgaggcgag ccccgcggag ctcgcgccgc gcctctgcgg ctcgccggat gtcttcgcgc
     2041 gccgcggccg acaaccgtgg tcgagcgtca acttcattac cgcgcatgac gggttcacgc
     2101 tttacgacct tgtctccttc aacgacaagc acaacgaggc gaacggggag ggcaaccgcg
     2161 acggccattc gcacaatagg tcctggaacc acggcttcga gggctcgacc gacgacgaag
     2221 cggttcgcgg tctgcgcaag cggcagatga agaacatgct tgcgacgctg ctcctttcgc
     2281 aaggcacccc gatgatcctt gccggcgacg agttcggccg cagccagcgc ggcaataaca
     2341 acgcctactg ccaggacaac gagatttcct gggtgcgctg ggtgcacgac gaccgcgcgc
     2401 agcgcctgat ccggttcgtg aagaagctga tcggcctgcg cagccgctat ccgatcctgc
     2461 gccgcacgcg ctggctcacc ggccagtacg acgagcaact cggggtcaag gacgttacct
     2521 ggatcaatgc caccggcgac gagatgcgcg aggagcattg gaaggacgcg aacgtgcgct
     2581 gcttcggcat gctgctcgac gggcgcgcgc agagcaccgg catccgcaag cgcggcgagg
     2641 aagccactct cctcatcatc ttcaacccct ggcaggacgt agtgaagttc aagctgcccg
     2701 gggcaaacgg cggcgagggc tggaagctgc tggccgacac caactttccc gacagcccgg
     2761 acgagccgga cttcgagccc ggccacgaat acgaagtcac cgcgcgctcg ctgctgcttt
     2821 ttcagctgac ctgaggcgct ggcgatgacg gcggccgcgt tccagacccg cggcgcgcga
     2881 ccgggattct ccaccgtgct cgcgctggtg gtggtcgccg cgatcctcca cctggcgcgt
     2941 gatgtcctgg tcccgctcgc gctggcgatc ctgctgagct ttctgctggc gccgggggtg
     3001 agcttcctcg agcgctgccg cctgggtcgc gtgcccgcga cggccgtcat cgtgatcctg
     3061 agcttctcga tcatcggcgc ggtcggctgg ttcgcggcga gccaggcggt gtcgctcgcc
     3121 gccaagctgc ccgaataccg ggaaaacatc agcgccaaga tccgcgcgct gcgtgcgccg
     3181 agcgacggca agctgggcca ggcagcggag gcgatcaggg agctcgagga cgaggcggct
     3241 ccggagcagc ccccgctcgc ggtgaaggag accgcggcta cgccgctggc ggcattgatc
     3301 gaatggacga cacccttcgc aaaaccgctc ggcctcgcgc ttgccgtgat catcttcacc
     3361 attctcttgc tgctgaaccg cgaagacatg cgcgaccggc tgatcggcct gatcggagcc
     3421 gggcgcatca acctcaccac gcaggcgttc ggcgaagcct cgggacgcgt gagccggtat
     3481 ctgttcatgc agctcgtggt caacgccggc ttcggcattc cgttcggcat cgcgctttac
     3541 atcatcggca tcccgaacgc gatgctctgg gggctgctcg ccatcttgct gcgtttcatt
     3601 ccctatgcgg ggatatggat cgccatcgcg atgccggtga cgctggcgtt cgcgatttcc
     3661 gacggctggt cgctggtggc ctggaccgtc ggcgtgttcg cagcgctcga gctgatcttc
     3721 gtctacggag tcgagccgtg gctctacggg cgcagcaccg ggctctcgcc catcgcgatc
     3781 atcgcggcgg tgatcttctg gacctggctt tggggcccgg tgggactgct cctcgccacc
     3841 ccgctcaccg tctgcgtcgc ggtgatgggg cgctatatcc cggagctcgg cttcctcaac
     3901 gtgctcctcg gcgttgagcc ggttctcacg cccgaagccc gcctgtacca gcgcctgatc
     3961 gcgctcgata gtgacgaagc gatcgacgtc gccgaggaat acgcaaacga gcacggactg
     4021 ctcgccctgc acgaggccgt cgtcatcccc gcgcttgcgc tggccgagca tgaccgccac
     4081 cgcggcgtca tcgacggccc gcgcgagcgc ttcgtgttcg ataccgtccg gaaaatcatc
     4141 gaggatgtcg aggaccgcaa ggcgatgaag gaggacaccg agcacacgtc ggaggacggc
     4201 gaagcgaagc ccgctgttcc ggccgtggcg gccgcgcctc cgtcgcgtgc ccctacggcg
     4261 ctatgcatcg cgccggcgca cgacgaagcc gatcacctcg caggcctggc cttcgctcga
     4321 actctgccgc cggccgattt cgacgtgcag gtcatcggct atcctgtcct tgccggcgag
     4381 atcgtcgagc agattgccaa atccggcgcc aaaattgcct gtatctcggc ggttccgccc
     4441 caagccgtgg ttcaagccag ctacatcgtc aagcgcctca agagccgcgt tcccggtgtc
     4501 aagacggtcg tcgtcatctg gaccaaggaa gacattgcga gggcacgctc gcgcctcggc
     4561 gacgccggcg ccgacaagat cgtctccaac attcccgatg ccattgcaca gctccacgag
     4621 ctctcggtcc aatgaaagca agcaagctgc cgctcgccgt gctcgccacc gccggcgttg
     4681 ctcttgcagc cacggtcgtg gcggcgaatt tcagccaccg cgagaagcga atcggtcagc
     4741 gaattaagcg cctctattcc gcggcggatc cggatttcgc gcgcgcgatg ggggtattgc
     4801 tcggacccgc gatcgtggac ggcaaccgct tcgcggtgct gctgaacggc gatcagatct
     4861 tccccgccat gctcgccgcg atccggcaag cgcggaagag catcaccttc gaaagctaca
     4921 tctactggtc ggggacggtc ggcagcgagt tcgccgaggc tctcgccgaa cgttcgcgcg
     4981 ccggggtgaa ggtgcacgta ttgctggact ggattggcag caacaaaatg gatcccgcgc
     5041 agctcgcccg gatggagcag gctggcatag aggtgcgcag gttccacgcg ccccattggt
     5101 acaacctcgc gcgggtgaac aaccgcaccc accgcaaggt gctggtggtg gacggtgaga
     5161 tcggtttcac gggcggtgta ggcatcgccg accagtggac cggcgctgcg caggatgcgg
     5221 agcactggcg cgacacgcat ttccgcgccg aaggaccggt cgttgcgcag atgcaggccg
     5281 tgttcctcga caactggatc aaggccaccg gggtcgttct gcacggcgcg gactacttcc
     5341 cagaactcaa acctcgaggc acgggttcag cgcagatgtt tagcagctcg cgctcgggcg
     5401 gcagcgagag catgcagctc atgtacttgc ttgcgattac ggcagcgcag cgctcgatcc
     5461 acctgtccac cgcctatttc gttcccgact cgctgacact gcgaacgctt gcggatgccg
     5521 tcaagcgcgg cgtgaaagtg cagatcatca ccccgggcga gcatacggac gccgaaatcg
     5581 tgcagcacgc atcgcgcgca cgctggggcg ccctgctcga ggctggcgcc gagatctacg
     5641 aatatgtgcc cacgatgtat cactgcaaag tcatgatcgt cgatgcgctg ctggtgtcgg
     5701 tgggttccac caacttcgac aaccgctcct tccagctcaa cgacgaggcg aatctgaata
     5761 tctacgatgc cgagttcgct gcccggcaag tcgagatctt tgaggacgac ctgcgccagt
     5821 cgcgtcggat cagcctggcg gaatggcggc agcggccgcg tgtggagaag ctcttggatc
     5881 gcacgtcgtc cttgttcggt tcgcagatgt agcggtctcg agctgcctca gccaccggcg
     5941 cgcgccgatc aagccgccaa ctcgcctccg ggctggcttc aaaagagcgc gcttagcgtc
     6001 ctatagggcc ggcgcccggg tagacctgcg aagcgtgctc ggcgtcgcgc tcggtgcgca
     6061 cagccccgcc gtgcgcgcgc tcgctcaacc ggcgcttcgc acgccctagc tgctcgcgca
     6121 gctgggcccg ccgttgcggc cccagttccc gatccatgag atacatcgcc aaagcgcctg
     6181 cgccgagtag aagtagagtt ttcatgaggc cctacaaagc aatattcgga ccgtaacgag
     6241 cctcatagga gcggatgggc ccgaaaatgg gtatcttttt gcggtgttca tcgaagcttg
     6301 cgagtcggca gcgcatcccg gctggctgcg cctgcgcgaa gcgctgtggc cggactgcgc
     6361 cagggcgaaa catctagccg agatgaattc cttcgtcgcg cagcctgagc gctacgtgca
     6421 gttcctggcc tactccccta cgcacgaagc gatcgggttc gccgagctgt cgctgcgaag
     6481 cgattatgtg aacggcaccg agagctcacc ggtcgccttc ctcgagggcc tctatgtggt
     6541 gccgcaagca cggcgcaaag gcgtggcgcg cgcgctcgtc gcggtggcga gcgagtgggg
     6601 ccgccgcgcc ggctgcggcg agcttgcgtc ggacgcggtg ctcgaaaatt ccatcagcca
     6661 tttggtgcat cgcgcgctcg gcttcgaaga aaccgagcgc gtcgtctact tccggaggaa
     6721 gctcgcatga aataccaggg cggttgtcat tgcggcggta tcaagttcga agtggagggc
     6781 gaactgaaag gcgttatggc gtgcaactgc tccatctgca cgcgcaaggg cgcgctgctc
     6841 tggttcatcc cgcgcgagaa cttccattgg ctgacgccgg agaaggccgc taacgcctac
     6901 accttcaaca agcacaggat caagcaccgc ttctgcccga actgcggcat ccacacgcac
     6961 ggcgaggcgt ccgacccttc gggcaagccc atggcggcga tcaatacgcg ctgcctcgaa
     7021 ggcatcgacc tcgccaagct gccggtcaag cacttcgacg gccgctcgct ttgagaaccg
     7081 gcggatgcct gtgcggcgcg gtacgctatc aaacgcctct gaaagcggcg cgcacggttg
     7141 cgtgtcactg caagttctgc cagcgttgca cgggaagcgc gttcgccgtg tggccgacgt
     7201 tcgcgagggg tgaagtggag ctgagcggtg cgctcacttc gtacgagcac cgctccgacg
     7261 agagcgggcg ctggatccgg ctggacttct gcccgcgctg cggcacgacg gtgacatcga
     7321 cgttcgaaaa gggtcccgca gagatcgcga tccttgcggg cacgttcgac gcgaccgact
     7381 ggattacggt ggaccgccat gtctggacgc gatcaaagca cgactggatc tcgattccgg
     7441 cgggcgtgcc ggcctacgaa agaagctcga ccgcgtgatc cgctacgcgc tgtgcttggt
     7501 cgcaacgaac gcgctggcgc agccctgcga gaaccccgta atcccgaagg cgaaggatcc
     7561 cgcggcgctg gtacaggcgg agcatcaatg gctgcgtgac gcctacggcg gcggcacgct
     7621 ggtgcgccag gtgctcggcg cctcgagcga cggccggcgc cgctacgatg tgattacctg
     7681 gcgcaagccc gacggcgaga ccgtggaagt atgcttcgac gtcacaaccg tattcgtaga
     7741 aacgatccgg gaggtggagg aagaggaagc gggtgctgcg cgtcgtccgg ctcggtagtc
     7801 cgcgtctcgc cggcgagggc ttgcgcattg gcgtggcgcg ccggccgccg cgcggggtaa
     7861 agaaaagcga ctacgcgcgc cgtgatttct acgacgtctg gctgcccgag ctcgcgccga
     7921 gccagaagca ggtgagctgg gcgcttgcgc agccgtggac cgacgggcgc tggcgaagat
     7981 atgagaaggc atatcgcgcc gagatgaagg ctccggccgc gcagcgcctg atcgagctcc
     8041 tcgcgcgact ttcgcagcat gctgatttct cggtcggctg ctattgcgag cgcgaggacc
     8101 gctgccaccg ctcgatcctc aagaagctcc tcgcgcaacg tggcgcacgg ctctgaggcg
     8161 ccctcgtcat gggtggtacg ctgggcggcc ctcgtcaagc acgggccggt gctcgacgtg
     8221 gcgagcggtg caggaaggca tgcgcgcttc ttcgccgaac gcaacctcgc ggtggtcgcg
     8281 gtggatcggc agcggcagtc gatacccggg gtgaagttcg tgcaggcgga cctcgaggac
     8341 ggcagccctt ggccgtttcc cggcgagcgc ttcggcgcca tcgtcgtgac gaactacctg
     8401 catcggccgc tcctgccaat gatcggggca tcgctgggcg caggcggcgt actcatctat
     8461 gagacgttca tgatcggcaa cgagcgctac ggcaagccct ccaacccgaa cttcctgctc
     8521 cggcccggtg agctcctgca aagctttgcg gagctcacgg tgctcggctt cgagcaggga
     8581 acgacggggg gcgcggtcgt gcagcgcctg tgcgccatcc ggggcgaggc gagtagcgct
     8641 agaatccccg catgattacc ggaagcttgg tcgccatcat caccccgatg cacgaggacg
     8701 gccgcctcga cctcgcgcgc ttcagggatc tcatcgattg gcacgtggcg gagggcaccg
     8761 acggcatcgt ggtggtcggc accaccggcg agtcgccaac ggtgaacttc gatgagcaca
     8821 aggagctcat ccggatcgcg gtccagcatt cgcgcgggcg cattccgatc gtcgccggca
     8881 ccggcggcaa ttcgacggcg gaagcggtcg agctcaccga gtcggcgaag aaggccggcg
     8941 cgacggcttg cctgtcggtc gtgccgtact acaacaagcc gacgcaggaa ggcctgtacc
     9001 ggcatttccg caagatcgcc gaggcggtcg atctgccgat gatcctttac aacgtgcccg
     9061 gacgcaccgt ggccgatctg cagaccgaca cggtgctgcg gctggcgcag gtgcccggga
     9121 tcatcggcat caaggaagcc accgcgaaca tcgagcgcaa caccgatctc atccgccgcg
     9181 cgccgcgcga cttcgccatc tactccggcg acgactccac ctgcctcgcg ctcatcctga
     9241 tgggtggcca tggcgtgatc tcggtaaccg cgaatgtcgc gcccaagcgc atgcatcaga
     9301 tgtgcgccgc tgcgctcgtc ggcgacgtga agaaagcgcg cgagctcaac ttcaagctgt
     9361 ttccgctgca ccagaagctc ttcgtcgagg cgaacccgat cccggtcaag tgggcgctcg
     9421 ccgagatggg catgatcgag ccggggctgc gcctgccact ctcgccgctc gccgagaagt
     9481 tccatgagac ggtgcgcgaa gcgctgcagg aagcgggcat cgcgctgccg ggtttgcggg
     9541 tcgtccaagg ccggggttga tgcgcaaggc gctgctagcc acggccgtcg ctgcggccgt
     9601 gctgcccggc tgctcgtggt tcagcgactc ctcctccgtg gactacaagt ccgccggcaa
     9661 ggtgccgccg ctcgaggtgc cgccggacct cacctcgccc caacgcgaca accgctacgc
     9721 gattccggag caggcgccgc gctcgagcgc gacgctctcc ggctacgaag ccgagcgccg
     9781 ggagccggcg aagaatcctg gcgctgccac tcccggcgcg gcggcgcagc cggtgctcct
     9841 gccgcaggtc gagcggatca aggtggagcg cgcgggcacg cagcgctggc tcgtggtgca
     9901 ggagccgccc gagaagctgt ggcccctagt caaggaattc tggaccgaca acggcttcgc
     9961 catccgcatg gagctgccgg aagccggcgt gatggaaacc gactgggcgg agagtcgcgc
    10021 ccgcatcgac gaaggcgcca tgcgcggatt gctcagccgc gcgctcgatt atttccattc
    10081 cacctccgag cgcgacaagt accgcacgcg gcttgaccga acgcccgacg gcgccagcga
    10141 gatctacatc agccaccgcg gcatgcagga ggtctacacc accgcgcgcg acccgacctc
    10201 gagcgcgccg ggtgcgacgg cgtggcagcc gcgtccttcg gatccggagc tcgaggcgga
    10261 attcctgcgt cgcctgatgg tcaagctcgg cacaccgggc gagcgcgcga agcagctgac
    10321 cgcgagcgca gagccgcaga tgcgcgccga gatcgtgaag tccaacgacg gcacggagcg
    10381 gctgcaggtg tacgaggcct tcgatcgcgc ctggcggcgc gtgggccttg cgctcgaccg
    10441 cgtcggcttc accgtcgagg atcgcgaccg ccagaagggc ctgtacttcg tgcgctacgc
    10501 cgatccggaa tccgaggcgg ccgcgagcga ccccggtttt cttggccgca tcttcggctc
    10561 cggctccaag gtgaaagccg agcagtaccg cgtgcaggtc gcccaacagg cgaacggcag
    10621 ccaggtgctg gtcctgaaca aggacggcgg cgccgagcgt tcgaagacgg cgcagcgcat
    10681 cctcacgctc ttgcacgagc agcttaagta acgtttgcgc ttcgcctcgc tcgcgagcgg
    10741 cagctcggga aactgcctgg tcgccgaggc ggacggcacc gtgctactcg tcgactgcgg
    10801 cctcgcactc accgagaccg agcgccggtt gcagcgcctc ggcctcgagc cttcgcaggt
    10861 gagtggtatc ctcattacgc acgagcacgg cgatcatgcc tgcggcgcct tcgacttcgc
    10921 cgcggcgcat cgcgtcacgg tgtacctcac gcacggcacg gctgcggcgc tgaaagcgga
    10981 agggaaggtg ctcgacggcg taccgctttg catcgtaaac gggcgccagg cgttcttcat
    11041 tgacggcctg cagctcgtgc cgttcacggt gccgcacgac gcgcgcgagc cgatccaatg
    11101 tgtggtgtcc gacggcgcgg cgaagctcgg cgtcctgacc gacgtcggca tgtcgacgcc
    11161 gcacgtcgag cgcatgcttg gtggcctgga cgcgctggtg ctcgagtgca actacgacgc
    11221 cgacatgctg tgggccggcg gctatccgcg ctggctgaag gaacgcatcg ccggaccgtt
    11281 cggccatctc gacaaccgcg agtccgagcg catcctcgcg gcgattgacc gctcgaagtt
    11341 gaagcacatc atcggcgcgc acctctcgca ggcgaacaat cgccccgagc tcgcgcgcgc
    11401 ggctctcgcg cgcgcgctcg gctgcgccga aagctggatc agcctcgcca cccaggccga
    11461 cggctttggg tggcggcagc tgtaggacta cttcttcata ggatccgtct tcttctccgc
    11521 cggcggcggc gtagaggcgc cggtcgtgcc tttcttcgag gcgtcgtcgc cgcaggcggc
    11581 gaggaaaatt gcgagtgcaa tcgctggcag cagcagtttc ataagaagct cctctctccg
    11641 atgacgcggc cattctagcg agcgccacta gctcgctccc tcgtcaggtt cgcgtcagtc
    11701 tcgcaactgt cttaaaggcc gatggtggtg ctcgacaccg ccgggtatga ggcggcccag
    11761 atctcaccga agcggccacg cagcgcctga
//
LOCUS       DASTPD010000103         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_52030, whole genome shotgun sequence.
ACCESSION   DASTPD010000103 DASTPD010000000
VERSION     DASTPD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52030"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2655
                     /locus_tag="VFR57_03710"
     CDS             <1..2655
                     /locus_tag="VFR57_03710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEU0258513.1"
                     /translation="FAALEAGFIPAGRINSAAGVQLQATLINCFVQPVGDSISEVVDG
                     RPGIYTALQEAAETMRRGGGVGYDFSTIRPKGAEVKGTRSRASGPVSYMRVFDRSCET
                     VESAGSRRGAQMGVLRCDHPDIEEFIHAKDHGDLANFNISVGVTDEFMAAVEKDLSFD
                     LVHKAKPWQTNEEARQREDGVWVYRTVRARDLWDQIMRSTYDHAEPGVVFLDRMNQDN
                     NLYYCESIEATNPCSEQPLPPYGCCCLGSIDLTKFVRNEFSERSFFDFERFGKVVAVA
                     VRMLDNVLDVTAWPLEQQRKEAMAKRRVGLGFTGLGDALIMLRLRYDGAEARAMAARI
                     SETMRDHAYRASAELAKERGAFPLFNADLYLSGSSFAMRLPEPVKDLIRNNGLRNSHL
                     LSIAPTGTISLAFADNASNGIEPPFSWTYTRKKRVDGGWQEYSVEDHAFRRYRAMGGD
                     VDALPPYFVTALELSAGAHEEMVAAVAPFIDSSISKTVNVPEDYPYSEFQDLYFLAWK
                     SGLKGLATYRPNKVLGSVLSVQKTEDFVQDDVNRRIQVKSVPAPVLASLRWPGRPDLS
                     TGNPAWTYMIEHPPYRFAVFVGHVENGRAHAFEVWVNGSEQPRGLGALAKTLSMDMRA
                     NDSAWLKLKLDALAKAGGDEPFEMPFPPRGEKKLMLSIVSAFAQIVRYRVEQLQAGLE
                     GEFSLSPEEASSPVLDAMFALKEPKTGTDGTMSWTVDVLNPRTGDDFVLGLKEITLPD
                     GITRPYSVWLSGEYPRALDGLCKILSLDMRVIDPAWIGMKLRKLLDFPEPLGDFMAFV
                     PGSRKQQNFPSTVSYVARLILHRYAMLGMLDENGAPRQQMGILESPVSREAPLLAGAK
                     CPECGNKALIRRDGCDYCTACGYVGICG"
     gene            complement(2811..3506)
                     /locus_tag="VFR57_03715"
     CDS             complement(2811..3506)
                     /locus_tag="VFR57_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0258514.1"
                     /translation="MIELRRSAERGHANHGWLDSYHSFSFADYYDPRHMGFGPLRVIN
                     EDRVQPGSGFGTHGHRDMEIISYVLEGALGHKDSMGNGSTIVPGDVQRMSAGTGVRHS
                     EFNHEKAGVTHFLQIWIEPDVVGIPPSYEQKHFEPEDKRGRLRLIASPDGSEGSVTVH
                     QDALLYAGLFDGKESASHSLRAGRKGYVHVARGEITVNGQMLEAGDALKTDGGAIELK
                     DGSNAEVLVFDLP"
     gene            complement(3545..4099)
                     /locus_tag="VFR57_03720"
     CDS             complement(3545..4099)
                     /locus_tag="VFR57_03720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0258515.1"
                     /translation="MKPSKPSDLTEHFVSGGLVFDGQLLKVYRDTVRLPDGTHGTREY
                     IRHPGAVAVVALFDDGAVLLERQYRYPARREFIEIPAGKLEPGEPHLDTAKRELLEET
                     GYVAAEWTPLGVIHTAIGYADETIELFLAKKLTKKEASLDAGEFLEVLKIPFDEAVAM
                     IRDGRILDSKSVVGLLWVDKWAAR"
     gene            complement(4077..>4496)
                     /locus_tag="VFR57_03725"
     CDS             complement(4077..>4496)
                     /locus_tag="VFR57_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0258516.1"
                     /translation="FGVLLYCSRAGFECENLDDVKGLNRTHPWVAFLMLLLMFSLAGI
                     PPMAGFYAKLAVLWAAVNAGYIWLAVAAVIFSLIGAFYYLRVVKLMYFDDPKDATRPV
                     GSTGFGVALSVNGLALLLLGLAPWPLMNACFEAIKAL"
BASE COUNT          794 a         1501 c         1444 g          757 t
ORIGIN      
        1 ttcgccgcgc tggaggccgg cttcatcccg gcgggccgga tcaactcggc cgcgggcgtg
       61 cagttgcagg cgacgctcat caactgcttc gtgcagccgg tcggcgactc gatctccgag
      121 gtggtcgacg gccggcccgg catctacacc gccctgcagg aggcggccga gaccatgcgc
      181 cgcggcggcg gcgtcggcta cgacttctcg accatccggc cgaagggggc cgaggtgaag
      241 ggcacgcgct cgcgcgcgag cggcccggtg tcgtacatgc gcgtcttcga tcgctcctgc
      301 gagacggtcg agtccgccgg ctcgcggcgc ggcgcccaga tgggcgtgct gcgctgcgac
      361 catccggaca tcgaggaatt catccacgcc aaggaccacg gcgacctcgc gaacttcaac
      421 atctcggtgg gcgtgaccga cgagttcatg gcggcggtgg aaaaggatct ctctttcgac
      481 ctggtgcaca aggcgaagcc ctggcagaca aatgaggaag cgcgccagcg cgaagacggc
      541 gtttgggtct accgcacggt gcgcgcgcgc gatctttggg accagatcat gcgctccacg
      601 tacgaccacg ccgagccggg cgtggttttc ctcgatcgca tgaatcagga caacaacctc
      661 tactactgcg agtcgatcga ggcgacgaac ccctgctccg agcaaccgct tccgccctac
      721 ggctgttgct gcctcggatc gatcgatctc accaagttcg taagaaacga attctcggag
      781 cgttccttct ttgatttcga gcgcttcggg aaagtggtcg ccgtcgcagt ccgcatgctg
      841 gacaacgtac tcgacgtcac cgcctggccg ctcgagcagc agcgcaagga ggcaatggcc
      901 aagcgccgcg tcggcctcgg cttcaccggt ctgggcgacg cgctcatcat gctgcgcctc
      961 aggtacgacg gcgccgaagc acgcgccatg gccgcgcgca tctccgagac catgcgcgac
     1021 catgcctatc gcgcctcggc ggagctggcg aaggagcgcg gcgcgttccc gctcttcaac
     1081 gccgatctct acctttccgg aagcagcttc gccatgcggc tccccgagcc ggtgaaggac
     1141 ctgatccgca acaacgggct gcgcaattcg cacctgctct cgatcgcgcc taccggcacg
     1201 atcagcctcg ccttcgccga taacgcctcc aacggcatcg agccgccctt ctcctggacc
     1261 tacacgcgca aaaaacgcgt cgacggcggc tggcaggaat actcggtcga ggaccatgct
     1321 ttccgtcgat accgcgcgat gggcggagac gtggacgcgc ttccgccgta cttcgtgacc
     1381 gcgctcgagc tcagcgccgg ggcgcacgag gaaatggtgg ctgccgtagc gccgttcatc
     1441 gacagcagca tctcgaaaac ggtgaacgtc cccgaagatt acccgtactc ggaattccag
     1501 gacctctact tcctcgcctg gaaatcgggc ctcaagggcc tcgcgaccta ccgtcccaac
     1561 aaagtcctcg gctccgttct ttctgtccag aagacggaag acttcgtcca ggacgatgtt
     1621 aaccgccgca tccaggtgaa gtcggtcccc gcgccagtgc tcgcctcgct gcgctggccc
     1681 ggccgcccgg atctctccac cggcaacccg gcgtggacct acatgatcga gcacccgccg
     1741 taccgcttcg cggtgttcgt cggccatgtc gagaacgggc gtgcgcatgc cttcgaagtc
     1801 tgggtgaacg gcagcgagca gccgcgcggc ctcggcgcgc tcgcgaaaac gctctcgatg
     1861 gacatgcgcg caaacgacag cgcgtggctc aagctcaagc tcgacgcact ggccaaggcg
     1921 ggcggcgacg agcccttcga aatgcccttc ccgccgcgcg gcgagaagaa gctcatgctc
     1981 tcgatcgtct ccgccttcgc ccagatcgtc cgctatcggg tcgagcagtt gcaggcagga
     2041 ctcgagggcg agttctcgct gtcgccggaa gaagcttcgt ccccggtcct cgacgccatg
     2101 ttcgcgctca aggagccgaa gaccggcacc gacggcacca tgtcctggac ggtggatgtc
     2161 ctcaacccgc gcaccggcga cgacttcgtc ctgggcctga aggagatcac cctgcccgac
     2221 ggcatcacgc gcccctattc cgtctggctc tcgggcgaat acccgcgcgc gctcgatggg
     2281 ctgtgcaaga tcctctcgct cgacatgcgc gtgatcgatc cggcgtggat cgggatgaag
     2341 ctgaggaagc tcctcgactt ccccgagccg ctcggcgact tcatggcttt cgtcccgggc
     2401 agccgtaagc agcagaactt cccctcgacc gtctcctacg tggcgcgcct aatcctgcac
     2461 cgctacgcca tgctcggcat gctggacgag aacggcgcgc ccaggcagca gatgggcatt
     2521 ctcgaaagcc cggtttccag ggaagccccg ctgttggccg gcgcgaaatg ccccgagtgc
     2581 ggcaacaaag cgctgatccg cagggacggc tgcgactact gcaccgcctg cggctatgta
     2641 ggtatctgcg gatagtgaaa atccaggcga gcacgtcgcg gtagtcgggc gtcttctcgc
     2701 acaccgagcg ggcgaaagga cagagcggca tcagccggcg gttgccggcg cgcgcccact
     2761 cgaccgccgc tttcaccagt agcgctgctc ctcgtgcgtc acgtccatgg ctacggcagg
     2821 tcgaaaacca gcacttcggc gttgcttccg tccttcagct cgatcgctcc gccgtcggtc
     2881 ttcagcgcat cgccggcctc gagcatctgg ccatttacgg tgatctcccc gcgggctacg
     2941 tgaacatagc ctttgcggcc agcacgcaag gaatggctcg ccgactcttt accgtcgaac
     3001 aggccggcgt aaagcagcgc atcctggtgg acggtcacgg agccttcgct cccatcgggc
     3061 gaggcgatga ggcgcaggcg gccacgcttg tcctcaggct cgaagtgctt ctgctcgtag
     3121 gaaggcggta tgccgacgac gtcgggctcg atccagatct gcaggaagtg cgtcaccccc
     3181 gccttctcat ggttgaactc gctgtggcgc acccccgtgc cggcgctcat gcgctgcacg
     3241 tcgcccggca cgatggtgga gccgttgccc atcgagtcct tgtggcccag cgcgccttcg
     3301 agcacgtagc tgatgatctc catgtcccgg tggccatggg tgccgaaacc agagccgggc
     3361 tgcacgcggt cctcattgat cacgcgcagc gggccgaagc ccatgtggcg cgggtcgtag
     3421 taatcggcaa aggaaaagct gtggtaggag tcgagccagc cgtggttggc gtgcccgcgc
     3481 tcggcgctgc ggcgaagttc gatcatcgcc ctaagtttgc ggcggctgcc gggttttgca
     3541 agccctagcg cgcggcccac ttgtcgaccc agagcagccc gaccacgctc ttcgagtcga
     3601 ggatgcggcc gtcgcggatc atggcgaccg cctcgtcgaa cgggatcttg agcacctcga
     3661 gaaattcgcc ggcgtcaagg cttgcttcct tcttcgtgag ttttttcgcc agaaagagct
     3721 cgatggtctc gtcggcgtag ccgatcgcgg tgtggatgac gccgaggggc gtccactcgg
     3781 cggcgacgta gccggtctct tcgaggagct cgcgcttcgc ggtatcgagg tgcggctcgc
     3841 ccggctcgag cttgccggcc gggatctcga tgaactcccg ccgggcggga tagcggtact
     3901 ggcgctcgag aaggaccgcg ccatcgtcga acaacgccac cacggcgacc gcgccggggt
     3961 ggcggatgta ttcgcgagtg ccgtgcgtgc cgtcgggaag ccggacggta tcgcggtaga
     4021 ccttcagcag ctgaccgtcg aataccaggc cgccgctgac gaaatgctcg gtcaggtcag
     4081 agggctttga tggcttcaaa acaggcgttc atcagcggcc agggcgcgag gccaaggagg
     4141 agaagcgcaa ggccgttgac cgaaagcgcg acgccgaagc cggtgctgcc gacggggcgc
     4201 gtcgcgtcct tgggatcatc gaagtacatg agcttcacga cgcgcaggta gtagaaggcg
     4261 ccgatgagcg aaaagatgac cgccgcgacc gcgagccaga tatagccggc gttcaccgcc
     4321 gcccacagca ccgcgagctt ggcgtagaag ccggccatgg gcgggatgcc ggcgagcgaa
     4381 aacatgagaa gcagcatcag aaacgcgacc cagggatggg tgcggttcaa gcccttgacg
     4441 tcgtcgagat tctcgcactc gaacccggcg cgcgagcagt agagcagcac gccgaa
//
LOCUS       DASTPD010000104         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1074374, whole genome shotgun sequence.
ACCESSION   DASTPD010000104 DASTPD010000000
VERSION     DASTPD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074374"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFR57_03730"
     CDS             complement(<1..384)
                     /locus_tag="VFR57_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0258517.1"
                     /translation="MNLSNSTFFGFRRENGRVGVRNHVLILPVDDLSNAAAQAVEHNV
                     KGTLAIPHPYGRLQFGADLELHFRTLIGAGSNPNVAAVVVICIEDTWAKKVADGIRAS
                     GKPVAHFGIEQHGDHDTIMRASKAAR"
     gene            complement(387..668)
                     /locus_tag="VFR57_03735"
     CDS             complement(387..668)
                     /locus_tag="VFR57_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0258518.1"
                     /translation="MIHFVVHEEGDGVGVVVVEGVKSGQSLNGWIMEDDKDLEVTAKS
                     DIPIGHKVALRDYRPGDTVIKYGVDIGKVVQPIRKGEHLHVHNVKTKRW"
     gene            689..2197
                     /locus_tag="VFR57_03740"
     CDS             689..2197
                     /locus_tag="VFR57_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU0258519.1"
                     /translation="MFDRRRFLKSLGALGVSTGIPKLTAYPFDLGVASGYPTAESVVL
                     WTRLTGDLAQLSIPVRWEVFADESLKSLVSGGEMSADPAWAHSLHVEVQGLEPERWYW
                     YRFSSGDARSPVGRTRTAPRADAAVDRLRFIFASCQQYEQGYYGAYRHVVTDAPDLVA
                     FLGDYIYESTWGKDHVRSHGSPEPYTLEDYRARHALYRSDPDLQAAHAACPWILTWDD
                     HEVDNDYADDRPEDGMGREAFLERRAAAYRAYFEHMPLPPRMRPEGPRMRIHTYLEWG
                     KLARFHIVDTRQHRSWQACPRPNRRGGSNTVDIEECPRLGTPGRSMLGRAQERWLARS
                     LAESRAAWNVVAQTTPMAQFDTKPGPGRLAWTDGWDGYPAARQRFLNFLSSKKIPNPV
                     VLSGDVHSFNACQLKLDFDDFAAPVVASEFVTTSITSQAWAQERLNQYLPDNPHILLA
                     DSRFRGYTRADVSARRMQVDLRAVENVQRRETPCSTLASFVVEDGKPGPLRA"
BASE COUNT          378 a          794 c          738 g          421 t
ORIGIN      
        1 gcgcgccgcc ttcgacgccc gcatgatggt gtcgtggtcg ccgtgctgct cgatgccgaa
       61 atgcgccacc ggcttgccgc tcgcgcggat gccgtcggcg accttcttcg cccaggtgtc
      121 ctcgatgcag atcaccacca cggcggcgac gttcgggttg gagccggcgc cgatgagcgt
      181 cctgaaatgc agctccaggt cggcgccgaa ctgcagccgg ccgtacgggt gcgggatcgc
      241 gagcgtcccc ttgacgttgt gctccacggc ttgcgcggct gcgttcgaga ggtcgtcgac
      301 cggcaggatg aggacgtggt tgcgcacgcc gactcttcca ttttcccttc tgaagccgaa
      361 aaaagtggag ttcgagagat tcatgtctac caacgcttgg ttttcacgtt atgcacatgc
      421 agatgctcac ccttgcggat cggctgcacc acctttccga tgtcgacgcc gtacttgatc
      481 acggtgtcgc cgggccggta gtcgcgcagc gcgaccttgt ggccgatcgg gatgtcgctc
      541 ttcgccgtga cctcgaggtc cttgtcgtcc tccatgatcc agccattgag cgactggccg
      601 gatttgacgc cttccaccac gacgacgccg accccgtcgc cttcctcgtg aaccacgaaa
      661 tggatcatag ggccgttatt ctattcggat gttcgaccgc cgccgctttc tgaaaagcct
      721 cggggcgctc ggcgtttcga ccggcattcc gaagttgacc gcctacccgt tcgacctcgg
      781 cgtcgcgtcc ggatatccga ccgccgagag cgtcgtgctc tggacgaggc tgaccggcga
      841 tctggcccag ttgtcgatcc cggtacgctg ggaagttttc gccgacgagt ccctcaagag
      901 tctagtctcg ggaggcgaaa tgtcggccga cccggcgtgg gctcattcgc tccacgttga
      961 agtgcagggt ctcgagccgg agcgctggta ctggtatcgc ttcagctcgg gcgatgcgcg
     1021 aagcccggtc gggcgtacgc gcaccgcgcc gcgcgccgac gcggcggtgg accgcttgcg
     1081 cttcatcttc gcctcgtgcc agcaatacga gcaaggctat tacggcgcct accggcatgt
     1141 ggtgaccgac gcgcccgacc tcgtggcctt ccttggcgac tacatttacg agtccacttg
     1201 gggcaaggat cacgtgcgca gccatgggag cccagagccg tacacgctcg aggactaccg
     1261 cgcgcgccat gcgttatatc gctcggaccc cgacctgcag gcggcgcacg cggcctgccc
     1321 gtggatcctg acctgggacg atcacgaggt cgacaacgac tacgccgacg accgccccga
     1381 ggacggcatg gggcgcgaag ctttcctcga gcgccgcgcg gcggcgtatc gcgcttactt
     1441 cgagcacatg ccgctgccgc cgcgcatgcg ccccgaaggg ccgcgcatgc ggatccacac
     1501 gtacctcgaa tggggcaagc tcgcgcgctt ccatatcgtc gatacgcgcc agcaccgttc
     1561 ctggcaggcg tgcccgcggc cgaaccggcg cggcggctcg aacacggtgg acatcgaaga
     1621 atgcccgcgg ctcggtacgc cgggacgctc gatgctcggg cgcgcacagg agcgctggct
     1681 cgcgcgctcg cttgcggaat cacgcgcggc ctggaacgtc gtcgcccaga ccacgccgat
     1741 ggcgcagttc gacaccaagc ccggtcccgg gcgccttgcg tggaccgacg ggtgggacgg
     1801 ctacccggcc gctcgtcaac gatttctaaa ttttctttct tccaagaaaa tacccaaccc
     1861 cgtggtgttg agcggggacg ttcactcctt caacgcctgt cagctcaagc tcgacttcga
     1921 cgatttcgcc gcgccggtgg tggcgagcga gttcgtgacc acgtccatca cctcgcaggc
     1981 ctgggcccag gaacgactga accagtacct gcccgacaat ccgcacatcc tgctcgccga
     2041 cagccgcttc cgcggctaca cccgtgcgga cgtcagcgcc agacgaatgc aagtcgacct
     2101 gcgcgcggtg gaaaacgtgc aacggcgcga aacgccctgc agcacgctcg cttcattcgt
     2161 cgtagaggac ggaaagcccg gtccgcttcg agcctgagcc tacttgcagc gaatgttgcc
     2221 gagcgagaga tcgagcggct tggtgcagtc ctgctcgctc acccggcgat cgggcgccat
     2281 ctccggcggg cggcggctgc cgtcaccgta agggagtccg ccgtgcacgg c
//
LOCUS       DASTPD010000105         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_462065, whole genome shotgun sequence.
ACCESSION   DASTPD010000105 DASTPD010000000
VERSION     DASTPD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462065"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFR57_03745"
     CDS             complement(<1..1325)
                     /locus_tag="VFR57_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0258520.1"
                     /translation="MNAPLIGARVQRKEDYRFLTGTGQYTDDVALPNQSHAVFVRSPH
                     AHARIGKISYEEARKCPGVLAVYTGADLAAAKVGGLPCGWLITDVNGKPMKEPPYPPL
                     AQGKVRHVGERVAVVVAETLEQARDAAEKVKVDYEELPAVIDLTKAKTSTPIHDIAGD
                     NTCYVWALGDKAAVDAAFANAAHVTKLDFWNNRLVPNAIEPRSANAVYSRADDGYTLY
                     VASQNPHVERLLMTAFVLGLPESKVRVVSPDVGGGFGSKIYLYGEDVVVTWAAKQLNR
                     PVKWTAERSESYVSDAHGRDHLTSAELALDKDGKFLAMRVRTTANLGAYLSTFASCIP
                     TILYATLLAGQYTTQHIYCEVTAVFTNTTPVDAYRGAGRPEATYVVERLVETAAREMQ
                     MSPVEIRKQNFIREFPYQTPVALCYDTGNYQATLDAALKLADVQNFSKR"
     gene            complement(1322..1801)
                     /locus_tag="VFR57_03750"
     CDS             complement(1322..1801)
                     /locus_tag="VFR57_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0258521.1"
                     /translation="MTIKVSMKVNGKPVSADVEARTLLVAYIRENLRLTGTHVGCDTA
                     QCGACTVLMNGRAIKSCSIVAMQAEGADIMTIEGVAKPDGTLHPMQAAFKENHGLQCG
                     FCTPGMVMNALDFAQSHPNPTEQEVREALEGNLCRCTGYHNIVKSIVAGARAMGGKI"
     gene            1878..>2519
                     /locus_tag="VFR57_03755"
     CDS             1878..>2519
                     /locus_tag="VFR57_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258522.1"
                     /translation="MRLLIVEDDAPLASGLQRILEAEGHAVDVTARGEEAVAAASNEK
                     FDLVILDIGLPQMDGFEVLRRLRSSVSDKSGPTPVLVLTARDAVEDRVRGLDLGADDY
                     MVKPFAMPELTARVRALLRRSQAHGGPRIAHGPLTLDTVARRAFLRNEPLELAAREWA
                     VLEVLLAKVEKIVSKEAIIQAVAGWGDDLSPNAIEVYVSRLRSKLEPAGIKIRT"
BASE COUNT          388 a          794 c          851 g          486 t
ORIGIN      
        1 tttctcttgc tgaagttctg aacgtccgcg agcttgagcg ctgcatccag ggtggcctgg
       61 tagttcccgg tgtcgtagca gagcgcgacc ggcgtctggt acgggaactc gcgaatgaag
      121 ttttgctttc ggatctcgac cgggctcatc tgcatctcgc gcgccgcagt ctcgaccagg
      181 cgctcgacca cgtaggtcgc ttccggccgg ccggcgccgc gataagcgtc gacgggcgtg
      241 gtgttggtga acaccgcggt cacctcgcag tagatgtgct gcgtcgtgta ctgcccggca
      301 agcagggtcg cgtacaggat ggtcgggatg cacgaggcga aggtcgacag atacgcgccg
      361 aggttcgcgg tcgtgcgcac gcgcatcgcg aggaacttgc cgtccttgtc gagcgcgagc
      421 tcggcgctcg tcaggtggtc gcggccgtgc gcgtcggaga cgtaggactc cgagcgctcg
      481 gcggtccact tcaccgggcg gttcagttgt ttcgccgccc aggtgaccac cacgtcttcg
      541 ccgtagaggt agatcttcga gccgaagccg ccgccgacgt cgggcgaaac cacgcgcacc
      601 ttgctctcgg gcagcccgag cacgaacgcg gtcatcagca gccgctcgac gtgcgggttc
      661 tggctcgcga cgtagagggt gtagccgtcg tcggcgcgcg aatacacggc gttcgccgag
      721 cgcggctcga tcgcgttcgg cacgaggcgg ttgttccaga agtcgagctt cgtcacatgg
      781 gctgcatttg caaaagcagc atcaacagcg gccttgtcgc cgagcgccca gacgtagcag
      841 gtgttgtccc cggcgatatc gtgaatcgga gtgctcgtct ttgccttggt aaggtcgatt
      901 acggcgggga gttcctcgta atcgaccttt accttttctg cggcgtcgcg tgcctgctcg
      961 agcgtctcgg cgaccactac cgcgacgcgc tcgccgacgt ggcgtacctt gccctgcgcg
     1021 agcggcgggt agggtggttc tttcatcggc ttgccgttca catcagtgat cagccagccg
     1081 cacggcagcc caccgacctt ggccgcggcc aggtctgcgc cggtgtacac cgcaaggaca
     1141 ccgggacact ttcttgcttc ttcgtacgag atttttccta ttcttgcgtg tgcgtgggga
     1201 gagcgcacga agaccgcgtg gctttgattg ggcaaagcca catcgtcggt gtactggccg
     1261 gtgccggtga ggaagcggta atcctccttg cgctggacgc gcgcgccaat aagcggggcg
     1321 ttcatatctt gcctcccatc gcccgcgcgc cggccacgat gctcttcacg atgttgtggt
     1381 agccggtgca gcggcaaagg ttgccctcga gcgcttcgcg cacttcctgc tcggtggggt
     1441 tgggatggct ttgggcgaag tcgagcgcgt tcatcaccat cccgggggtg cagaaaccgc
     1501 attgcagacc gtggttttcc ttgaacgcgg cctgcatggg gtgcagggtg ccatcgggct
     1561 ttgccacgcc ctcgatcgtc atgatgtcgg caccctcggc ctgcatcgcc acgatcgagc
     1621 agctcttgat cgcgcggccg ttcatgagca ccgtgcatgc gccacactgc gccgtgtcgc
     1681 agccgacgtg agtgccggtc agccgcaggt tctcgcgaat ataggcgaca agcagcgtgc
     1741 gcgcctcgac atcggcggaa acgggcttgc cattgacctt catggatacc ttgattgtca
     1801 tgcggggctc ctccggggag aaaaatatcg tagcgcggcg gtatgattta tgccactgcc
     1861 atttctcgcc gccggccatg cgattgctga tcgtggagga cgacgccccg cttgcatcgg
     1921 ggctgcagcg catcctcgag gccgaggggc acgcggtcga cgttaccgcg cgcggcgagg
     1981 aggcggtggc cgccgcgtcg aacgaaaagt tcgacctcgt tatcctggac atcgggctgc
     2041 cgcagatgga cggattcgag gtgctgcgcc ggctgcgctc cagtgtctcg gacaagagcg
     2101 ggccgacgcc ggtgctggtg cttacggcgc gtgacgcggt ggaagaccgc gtgcgcggcc
     2161 tcgacctcgg ggccgacgac tacatggtga agcccttcgc gatgcccgag ctcaccgcgc
     2221 gcgtgcgcgc cctcttgcgc cgctcgcagg cccacggcgg cccgcgcatc gctcacgggc
     2281 cgctgacgct cgatacggtc gcgagacggg ctttcctccg caatgaaccc ctggagctcg
     2341 cggcgcgcga gtgggcggtg ctcgaagtgc tcctcgccaa ggtggagaag atcgtttcga
     2401 aggaggcgat catccaggcg gtcgccggct ggggcgacga cctttcgccg aacgcgatcg
     2461 aggtctatgt ctcgcgcctg cgctccaagc tcgagccggc cggaatcaag atccgcacc
//
LOCUS       DASTPD010000106         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1280307, whole genome shotgun sequence.
ACCESSION   DASTPD010000106 DASTPD010000000
VERSION     DASTPD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280307"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2457
                     /locus_tag="VFR57_03760"
     CDS             <1..>2457
                     /locus_tag="VFR57_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0258523.1"
                     /translation="KEALERQTASARILRVIGSSPSDLQPVFDAILENAMHLCDASLA
                     TVGLYDGKYYRHVAQRGGSPEYEKFLFSGPFEPKPRYTIGHMIAERQPFHIVDYRLLP
                     DYIEGDPRSIATVELGGTRTYLAVPMLKDGHVVGGITVRRTEVRPFTQKQIDLVRTFA
                     DQAVIAVENARLFNETREALERQTATAEILRVISGSPTATQPVFESIVQKCHALYQDS
                     RVALLLIDEGRLNTRASTGYAFEPIPIDRESGIGACVLEGRTIHLPDLEAAAEQYPRL
                     RQLGIKHGYRSGMYAPLMREGRAIGGISVLRRVAGAFSEKDVALLTTFADQAVIAIEN
                     VRLFNETKEALEQQTAISEVLRVISSSPADIKPVLEVVASRAAHICDATDARIFVVEG
                     NFMRHASGFGDVPVRTDLVPITRASTSGRAVIDRAPVHVPDIEAESPDEFDHQIARRS
                     GWRSALAVPLMRETRALGAIVLRRREVRPFTEKQAALLATFADQAAIAIENVRLFNET
                     KEALERQTATGEILKVMSASLTDVQPVFDAVALNAARVCGVDDVAILQTEGVNLRRVS
                     HYGNVPVSPHSLVPVPPGSVNERVLSEHRTLHIPDTLAPSFTREFPGSQFPAMGVRAL
                     LATPLVRDGIAVGVIHLRRSEARPFTDKQIAMLETFADQAVIAIENVRLFNEIKDSLE
                     QQKASAEVLAAISSSIADTQPVFEKILRSCERLFAGKLVGINLLDPDGLIRIGAYQGP
                     GREELEKLQPFAPREGSGSGAAILHRRLIQYADAQNGEDTPLATRRGCAAIGVKSVVF
                     APMIWEERALGVIFVGRDFAG"
BASE COUNT          441 a          801 c          816 g          399 t
ORIGIN      
        1 aaggaggcgc tcgagcgcca gactgcgagc gcccgcatat tgcgcgtcat cggcagctct
       61 ccaagcgacc tgcagccggt attcgacgcg atcctggaaa acgcgatgca cctctgcgac
      121 gcgagcctcg cgacggtcgg cttgtacgac ggaaaatatt atcgccacgt cgcgcagcgc
      181 ggcggcagtc ccgagtacga gaagttcctc ttcagcggac cgttcgagcc caagccgcgg
      241 tacacgatcg ggcacatgat cgccgaacgg cagcccttcc acatcgtcga ttatcgcctg
      301 ctgccggatt acatcgaggg cgatccccga tcaatcgcga cggtggagct cgggggaacg
      361 cgcacgtact tggccgtgcc gatgctcaag gacgggcacg tggtcggcgg aattaccgtt
      421 cgccgcacgg aagtgcggcc attcacgcag aagcagattg atctggtgcg cacgttcgcc
      481 gaccaggcag tcatcgcggt cgagaacgcg cgactgttca acgagaccag ggaggcgctc
      541 gagcggcaga cggcgacggc cgagatcctg agggtcatca gcggctcgcc gacggcgacg
      601 cagcccgtgt tcgagtcgat cgtgcagaaa tgccatgcgc tgtatcagga cagccgggtc
      661 gcgctattgc tcatcgacga gggcaggcta aacacgcgcg ccagcaccgg gtatgcgttc
      721 gagcccatac cgatcgaccg cgagagcggc atcggcgcct gcgtgctgga gggacgcacg
      781 atccatctcc ccgacctgga ggcggcggcc gagcagtatc cgcgcttgag gcagcttggg
      841 atcaagcacg gctaccgatc ggggatgtat gcgccgctca tgcgggaagg acgcgcgatc
      901 ggcggcatct cggtgctgcg gcgcgtggcc ggcgctttca gcgagaagga cgtggcgctg
      961 ctcaccacgt tcgccgacca ggcggtgatc gccatcgaga atgtgcgact cttcaacgag
     1021 acaaaggagg ccctcgagca gcagacggcg atcagcgaag tgctgcgcgt gatctccagc
     1081 tcacccgccg acatcaagcc ggtgctcgag gtggtcgcat cgcgagccgc ccacatctgc
     1141 gatgcaacgg acgcccgcat cttcgtcgtc gaaggcaact tcatgcgaca cgcgagcggc
     1201 tttggcgatg ttccggtcag gaccgacctt gtcccgatta cgcgggcttc gacgagcgga
     1261 cgcgccgtga tcgatcgcgc accggtgcac gttccagata tcgaggcgga gtccccggat
     1321 gagttcgacc accagatcgc gcgcaggtcg ggctggcgct cggcgctcgc cgtcccgctg
     1381 atgcgcgaga cgcgcgcgct cggcgccatt gtcctgcgac gacgggaggt gcggcccttc
     1441 accgaaaaac aggcggccct tcttgcgacc ttcgcggatc aggcggcgat cgcgatcgag
     1501 aacgtgcgcc tgttcaacga gacgaaggag gcgctcgagc gacagacggc caccggcgag
     1561 atcctgaagg tgatgagcgc ctcgcttacg gacgtgcagc cggtgttcga cgcggtggcc
     1621 cttaacgccg cccgcgtgtg tggcgtggac gacgtcgcca tcctgcagac cgagggcgta
     1681 aatcttcggc gcgtctcgca ctatggcaac gtgccggtct ccccgcacag cctcgtgccg
     1741 gtaccacccg ggagcgtcaa cgagcgcgtc ctctccgagc accgtaccct gcatatcccc
     1801 gatacgctgg cgccgtcctt cacacgggaa tttccgggct cgcagttccc ggccatgggc
     1861 gtgcgcgccc ttctcgctac accgctggtt cgcgacggca tcgcggtcgg cgtgattcac
     1921 ctgcggcgct cggaggcacg gccgttcacc gacaagcaga tcgcgatgct cgagaccttc
     1981 gccgaccagg cggtgattgc gatcgagaac gtgcgcctgt tcaacgagat caaggactcg
     2041 ctcgaacagc agaaggcctc cgcggaggtg ctcgcggcga tcagcagctc catcgctgat
     2101 acgcagcccg tgttcgaaaa gattcttcgc agctgcgagc gactcttcgc cgggaagctt
     2161 gtcggcatca acctccttga cccggacggg ctgatacgca tcggcgctta ccagggtccg
     2221 ggacgcgagg agctggagaa gctgcagccg ttcgcgccac gcgagggctc gggaagcgga
     2281 gcggccatcc ttcatcgccg gctgattcag tatgcggacg cgcagaacgg cgaggacacc
     2341 ccgttggcca cccggcgcgg ttgcgctgcg atcggcgtga agtcggtggt attcgccccc
     2401 atgatttggg aagagcgcgc cctcggagtg atcttcgtcg gccgcgactt cgccggc
//
LOCUS       DASTPD010000107         7069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_669040, whole genome shotgun sequence.
ACCESSION   DASTPD010000107 DASTPD010000000
VERSION     DASTPD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669040"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFR57_03765"
     CDS             <1..391
                     /locus_tag="VFR57_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258524.1"
                     /translation="LLRGIPLVTEPARSIGRLGFRRWYERRLIEGHAWLISCFLCALG
                     IAASLEGISFKRAESIPLLVFVFLGGVVCWYALQRYRAIMDEAERLGERSICGGCRAY
                     AAFKLIGDDPKISVRCRKCGNEWRLDD"
     gene            complement(392..1972)
                     /gene="aceB"
                     /locus_tag="VFR57_03770"
     CDS             complement(392..1972)
                     /gene="aceB"
                     /locus_tag="VFR57_03770"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU0258525.1"
                     /translation="MSGPIPQGVAISGSISKEFAQILSPETLDFVAKLHRKFESRRQE
                     LLARRAERQKQFDAGAKPDFLAETKSIRESDWQVAEQPKDMLDRRVEITGPTDRKMVI
                     NALNCGASTFMADFEDANCPTWHNMVDGQINLRDAVRRTITFEQGGKQYRLNDSTAVL
                     IPRPRGWHLDEKHVTVDGRPVAGGVFDFALFFFHNAKELLKRGSGPYFYLPKMESHLE
                     ARLWNDIFVFAQKELGIPVGTIRATALIETVVAAFEMDEILYELREHSAGLNIGRWDY
                     IFSCIKKFRANKDFCLADRAQVTMLAPFMRAYALLLVKTCHRRNAPAMGGMAAQIPIK
                     HDPVANEAALEKVRQDKLREVTDGCDGTWVAHPGLVPVAKAVFDKHMPTPNQYTRQRP
                     DVTVTAKQLLDFQPERPITEAGLRNNISVGIQYVAAWLAGNGCVPVYNLMEDAATAEI
                     SRSQIWQWIRSPKGVLEDGRKVTPELFAKLLGEELAKVRSAGAEGKLEEAAKLFEQIT
                     TDDQYVEFLTLPAYKLIG"
     gene            2077..3132
                     /locus_tag="VFR57_03775"
     CDS             2077..3132
                     /locus_tag="VFR57_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258526.1"
                     /translation="MKPYNVLILGASYGSLLATKILAAGHNATLVCLPAEVDAFNTAG
                     ARVRMPVKGKEGLVEVHSWKLPGKLSAEGPSTVKPAEYDLVALAMQEPQYRSAGVREL
                     MDAIAKAKVPCMSIMNMPPLPYLARIPGISVKELRSCYTDPTVWDGFDPQFMTLCSPD
                     PQAFRPPEEKLNVLQVRLPTNFKSARFPSDEHTAILKKLEADIEAARFDRMELPVKLK
                     VHDSVFVPLAKWAMLIAGNYRCVTKEGPRAIKDAVHSDINASRAVYTWVVNLCLLLGA
                     KESDLVPFDKYAAAAQSLGSPSSAARALYAGAPYIERVDRLVQAIAAQKGLRLPILDE
                     VVGLVDAKLEANRKKAA"
     gene            complement(3129..4535)
                     /gene="ntrC"
                     /locus_tag="VFR57_03780"
     CDS             complement(3129..4535)
                     /gene="ntrC"
                     /locus_tag="VFR57_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEU0258527.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIAFNSFSSAQEALEALSGA
                     TPEVLVSDIRMPGRSGLELLQTIKSRYPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDIDQAVDLIRRALDERKKEMQAAEPASATPEILGQAPAMQEVFRAIGRLSQSSATV
                     LITGESGTGKELVARALHRHSARGKQPFIAINTAAMPKDLLESELFGHERGSFTGAQQ
                     QRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQQLKVNVRVIAAT
                     HQNLEQRVREGTFREDLYHRLNVIRLRLPSLRERAEDIPLLARHFLARSAQQLGVEPK
                     RIADDALAHIARLEYPGNVRQLENLCHWLTVMAPGQVIDLGDLPQEFRGQIGGAASDW
                     VAALEQEAERRLARGDTGILDSLNRQFERALIQKALARTGGRRIDAATLLGMGRNTIT
                     RKIQELGIEDHNNSGTEP"
     gene            complement(4532..5557)
                     /gene="glnL"
                     /locus_tag="VFR57_03785"
     CDS             complement(4532..5557)
                     /gene="glnL"
                     /locus_tag="VFR57_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEU0258528.1"
                     /translation="MAASFAGLDLLATAVVVLDDEFVVRYANPAAENLLTTGARSMIG
                     QPFLGLFAERDDLERALEDARIIHWDYSARNVTYVRTGREPVPLSCTVTRIDAFGMAL
                     LAELRPISEQLRIEREARMESEQQANRELIRNLAHEIKNPLGGLRGSAQLLERELDRP
                     ELREYTQVIIKEADRLQALMDRMLTPHRAPRIEPLGFHEVLERVRSLVKAEFGIEIVR
                     DYDPSVPDIEGDREQLIQAVLNIARNAAQVGATNVVFRTRAVRQITILKQRYRLALEL
                     QVIDDGPGVPDEIHERIFNPLVSGREGGSGLGLSLAQTFVHYHHGVVEFESRPGRTIF
                     RILLPIR"
     gene            complement(5563..6063)
                     /locus_tag="VFR57_03790"
     CDS             complement(5563..6063)
                     /locus_tag="VFR57_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEU0258529.1"
                     /translation="MQRAWALGVLMLGLSAGAAHAQPKPQGVTEIFKCRAENGQVTYT
                     NDRRNAEKQKCELVTRQINVAPPLAKPQPQATSRAPSSFPRETPGQSQNARERQREVL
                     ERELAAEQNALNQARQALAEQETVRSGDERNYARVLDRLQPYKETIENHEKNIQALQR
                     ELANLK"
     gene            complement(6146..>7069)
                     /gene="glnA"
                     /locus_tag="VFR57_03795"
     CDS             complement(6146..>7069)
                     /gene="glnA"
                     /locus_tag="VFR57_03795"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU0258530.1"
                     /translation="IDYESGNMGHRAPIKGGYFPVPPIDTLGDIRNAMVLALEQQGVE
                     VEVHHHEVAAPGQCEIGTRFAPLVQRADWMQILKYTVWNTAASYGKTATFMPKPVVGD
                     NGSGMHCHQSVWKGGKNLFAGNGYAGLSDFALHYIGGIIKHAKSLNAITNPGTNSYKR
                     LVPGFEAPINLAYSARNRSAACRIPYASGTSGRRVEVRFPDPTANAYLAFAAMLMAGL
                     DGVQNKIHPGDPVDKDLYHLPPEEAKKVPNVCASLEEALKCLEDDHAFLTRGGVFSDD
                     FLESYIALKNDELTRFRMTTHPVEFDMYYAS"
BASE COUNT         1195 a         2215 c         2305 g         1354 t
ORIGIN      
        1 tttgctccgc ggcatccccc ttgtgacaga acctgcacgc tccatcggcc gactcggctt
       61 ccggcgctgg tacgagcgtc ggctgatcga aggccatgcc tggctgatct cgtgctttct
      121 ctgcgcgctc ggcatcgcgg cctcgctgga aggcatcagc ttcaagcgcg cagaatccat
      181 tccgctcctg gtgtttgtgt tcctgggagg agtcgtctgc tggtacgcgc tgcagcgcta
      241 tcgcgcgatc atggatgagg ccgagcgcct cggtgagcgg tcgatctgcg gcggttgtcg
      301 cgcctacgct gcgttcaagc tgattggtga cgacccaaag atcagcgtgc gctgccgcaa
      361 gtgcggcaac gagtggcggc tcgacgactg actagccgat gagcttgtac gcgggaagcg
      421 tgaggaactc gacgtactga tcgtcggttg tgatttgctc gaagagcttg gcggcttcct
      481 ccagcttgcc ttcggcgccg gctgagcgga cttttgcgag ctcttcgccg agcagctttg
      541 cgaaaagctc gggcgtgact ttacggccgt cttccagcac gcctttcggc gagcgaatcc
      601 actgccagat ctgcgagcgc gagatctcgg cggtggcggc atcttccata aggttgtaga
      661 ccggaacgca gccgttgccg gcgagccacg cggcgacgta ctggatgccg accgagatgt
      721 tgttgcgcag gccggcttcg gtgatgggtc gctcgggctg gaagtcgagc agctgcttcg
      781 ccgtcacggt cacatccggg cgctggcgcg tgtactggtt cggcgtcggc atgtgcttgt
      841 cgaacacggc cttcgcgacc ggcacgaggc cggggtgcgc gacccacgta ccgtcacagc
      901 cgtcggtcac ctcgcgcaac ttgtcctgac gcactttttc gagcgcggct tcgttcgcca
      961 cggggtcgtg cttgatcgga atctgagcag ccatgccgcc catggccggg gcgttgcgac
     1021 gatggcaggt ctttaccaga agcagcgcgt acgcgcgcat gaagggcgcg agcatcgtga
     1081 cctgcgcgcg gtcggcgagg cagaaatcct tattcgcgcg gaattttttg atgcacgaaa
     1141 agatgtagtc ccagcgcccg atgttgagtc cggcggagtg ctcgcgcagc tcgtacagga
     1201 tctcgtccat ctcgaacgcc gcgaccacgg tctcaatgag cgccgtcgcc cggatcgtgc
     1261 cgacggggat gccgagctcc ttctgcgcga agacgaaaat gtcgttccag agccgcgcct
     1321 cgagatgcga ctccatcttc ggcagataga agtacgggcc cgagccgcgc ttgaggagct
     1381 cttttgcgtt gtggaagaag aagagcgcga agtcgaagac gccgccggcg acaggtctgc
     1441 cgtcgacggt gacgtgcttt tcgtcgaggt gccagccgcg gggccgcggg atgagcaccg
     1501 cggtgctgtc gttcagcctg tattgcttgc cgccctgctc gaacgtaata gtgcgccgaa
     1561 ccgcgtcgcg cagattgatc tgcccgtcca ccatgttgtg ccacgtcgga cagttggcgt
     1621 cctcgaaatc cgccatgaac gtggacgcgc cgcagttcag cgcgttgatc accatcttgc
     1681 gatcggtcgg gccggtgatt tccacccggc gatcgagcat gtccttcggc tgctcggcga
     1741 cctgccagtc ggattcgcgg atcgacttcg tctcggcaag gaaatcgggc ttcgcgccgg
     1801 cgtcgaattg cttctgccgt tccgcgcgtc gcgcgagcag ctcctggcgt cgggattcga
     1861 acttgcggtg aagtttggcg acgaagtcca gcgtttcggg gctgaggatc tgcgcgaatt
     1921 ctttgctgat cgagccgctg atcgcgacgc cctgagggat cggcccggac atgtgcattg
     1981 caccaattct attgcacgaa tgtggcggcg caacatgatt tgctggcccg aagggagtac
     2041 cttccccttt caagcgacgg agcaccattt cacattatga aaccctacaa cgttctcatc
     2101 ttgggcgcct cgtacggctc ccttctcgcg accaagattc tcgccgcagg ccataacgcg
     2161 acgctggttt gcctgcctgc cgaagtcgac gcgttcaaca ccgcaggcgc gcgggtgcgc
     2221 atgccggtca aagggaaaga ggggctggtt gaagtgcatt cctggaagct gcccggcaag
     2281 ctctccgcag aaggtccgag cacagtaaag ccggccgaat acgacctcgt cgcgctcgcg
     2341 atgcaggagc cgcaataccg ttcagccggc gtgcgcgagc tcatggatgc tatcgccaag
     2401 gcgaaagtgc cgtgcatgtc catcatgaac atgccgccgc ttccgtacct ggcgcggatt
     2461 ccgggcatct cggtgaagga actgcgctcg tgctataccg accctacggt gtgggacggc
     2521 ttcgatccgc aattcatgac gctgtgcagt cccgacccgc aggccttccg tccgccggaa
     2581 gagaaactga acgtccttca ggtgcggttg ccgactaact tcaagtcggc gcgcttcccg
     2641 tccgacgagc acaccgcgat cctgaagaag ctcgaggccg atatcgaggc cgcgcgcttc
     2701 gacaggatgg agctaccggt gaagcttaag gtgcacgact cggtgtttgt gccgctcgcc
     2761 aagtgggcga tgctcatcgc cggtaactat cgctgcgtaa ccaaggaagg accgcgcgcc
     2821 atcaaggacg cggtgcactc cgacatcaac gcttcgcgcg ccgtatatac ctgggtggtg
     2881 aacctgtgcc tgctgctcgg cgccaaagag tcggatctgg tgccgttcga caaatacgcc
     2941 gccgcggcgc aaagcctcgg cagcccgtcg tcggccgcgc gcgcgcttta tgccggcgcg
     3001 ccctacatcg agcgagtgga tcgcctggtt caggccatcg cggcgcagaa gggattacgg
     3061 ctgccgatcc tcgacgaggt cgtcggcctg gtggacgcga agctcgaggc gaaccgcaag
     3121 aaagccgcct agggctccgt ccccgaattg ttatggtcct cgattccgag ctcctggatt
     3181 tttcttgtaa tcgtatttct tcccattcct agaagagtcg ccgcgtcgat gcgtcggccc
     3241 ccggtgcgcg cgagcgcttt ctggatgagc gcgcgctcga actgacggtt gagcgaatcc
     3301 aggatcccgg tgtcgccccg cgccaggcga cgctcggctt cctgttcgag ggccgccacc
     3361 cagtcggaag cagcgccccc gatctggccg cggaactcct gcggcagatc gccgaggtcg
     3421 atgacctggc cgggcgccat caccgtgagc caatggcaga ggttctcgag ctgccgcacg
     3481 ttgcccggat attccagcct cgcgatgtgg gcgagcgcgt catcggcgat gcgtttcggc
     3541 tccacaccga gttgttgcgc gctgcgcgcg aggaagtgcc tggcgaggag cggaatatct
     3601 tcggcccgct cgcgcaggct gggcaagcgc agccggatga cgttcaagcg gtggtagagg
     3661 tcctcgcgga acgttccctc gcgcacgcgc tgctccagat tctgatgcgt ggcggcgatc
     3721 acgcgcacat tgaccttgag ctgttgatgt ccgccgacgc gatagaaggt gccatcggaa
     3781 agcacgcgca aaaggcgcgt ctgcaactcg gcgggcatgt caccgatttc gtcgagaaag
     3841 agagtcccgc cttcggcctg ttcgaagcgg ccgcgacgct gctgctgagc gcctgtaaag
     3901 gagccgcgct cgtggccgaa gagctcggac tcgaggagat ctttcggcat ggcggccgtg
     3961 ttgatcgcaa tgaacggttg cttgccgcgt gcactgtggc ggtgcagcgc acgggcgaca
     4021 agctcctttc cggttccgga ctcgccggtg atgagcaccg tcgcgctcga ttgcgacagg
     4081 cgtccgatcg cgcggaatac ctcctgcatc gccggcgcct ggccgaggat ctccggcgtg
     4141 gcggacgcag gctcggccgc ttgcatttcc ttcttgcgtt cgtctaacgc gcggcgaatg
     4201 agatcaacgg cctggtcgat gtcgaaaggc ttcggcaggt attcgtacgc gccgccctgg
     4261 aacgcggcga ccgcgctgtc cagatccgag tacgcagtca tgacgatcac cggcacggcg
     4321 ggatagcggc ttttgatcgt ctggagaagc tcgagccccg agcggcccgg catgcggatg
     4381 tcggagacaa ggacctcagg cgtcgcaccg gagagcgctt ccagcgcttc ctgcgccgag
     4441 gaaaaggaat tgaaggcgat gccttcccgg ctgagggcct tttcgatcac ccagcggatg
     4501 gagcggtcgt cgtcgacgat ccagaccggt ttcatctgat cggtagcagg atcctaaata
     4561 tggttcggcc cggccgcgac tcgaactcga cgacgccgtg gtgatagtgc acgaacgtct
     4621 gcgcgagaga gagcccgagc cctgagccgc cttccctgcc cgaaacaagc ggattgaaga
     4681 tccgttcgtg gatctcgtcc ggcacgcccg ggccgtcgtc gatcacttgc aattctaatg
     4741 ccagcctgta gcgctgcttg aggatcgtga tctgccgcac cgctctcgtc cggaagacga
     4801 cattggtggc accgacctgc gcggcgttgc gcgcgatatt gagcaccgcc tgaatgagct
     4861 gctcgcgatc gccttcgatg tcgggaacgc tcggatcgta gtcgcgcacg atctcgatgc
     4921 cgaattccgc cttcaccagg cttcgcacgc gctcgagcac ctcgtggaag ccgagcggct
     4981 cgatgcgcgg cgcgcgatgc ggcgtcagca tgcgatccat gagcgcctgc aggcgatcgg
     5041 cctccttgat gatgacctgc gtgtactcgc gcagctctgg ccgatcaagc tcgcgctcca
     5101 aaagctgcgc cgacccgcgc aacccaccga gcggattctt gatctcgtgc gcgagattgc
     5161 ggatcagctc gcgattcgcc tgctgctccg actccatgcg cgcttcgcgc tcgatgcgca
     5221 gctgctcgct gatcgggcgc agctccgcaa gcagcgccat gccgaaagcg tctatgcgcg
     5281 taacggtgca cgagagaggg acaggctcgc gtccagtgcg cacgtaggta acgttgcgcg
     5341 ccgaataatc ccaatggatg atgcgggcat cctcgagcgc gcgctccaga tcgtcgcgct
     5401 cggcgaacaa accgagaaaa ggctgcccga tcatgctgcg cgcgccggtg gtgaggaggt
     5461 tttcggcggc ggggttggcg tagcgcacga cgaactcgtc atcgagcacg acaacggccg
     5521 tggccagcag gtcaaggccg gcgaacgagg cggccacaga cgctacttca ggtttgcgag
     5581 ctcgcgctgc agcgcctgga tattcttctc gtggttctcg atcgtttctt tgtacggttg
     5641 cagccgatcg aggacgcgcg cgtagttgcg ctcgtcgccg gagcgcacgg tctcctgctc
     5701 ggcgagcgcc tggcgcgcct gattcagcgc gttctgctcg gcggcaagct cgcgctcgag
     5761 aacctcgcgt tgacgttcgc gcgcgttctg actctggccc ggggtctcgc gcggaaaaga
     5821 agaaggagcg cgcgacgtcg cctgaggctg aggcttcgcc aacggcggcg cgacgttgat
     5881 ctgtctcgtc acgagctcgc acttctgctt ttctgcgttg cggcggtcgt tggtgtaagt
     5941 cacctgcccg ttttccgcgc ggcacttgaa aatttccgtc acgccttgcg gttttggctg
     6001 agcgtgcgcc gcgccggccg acaggccaag catcagaacc ccgagcgccc atgcacgctg
     6061 catgggccaa gtctaccaag ccgaggcgca agccggaaaa tgaacgggga aacggcgtca
     6121 caccccgtct ccccgcggta caaaatcacg acgcgtagta catgtcgaac tcgaccgggt
     6181 gcgtggtcat gcggaaccgg gtgagctcgt cgttcttgag cgcgatgtag gactcgagga
     6241 agtcgtccga aaacacaccg ccgcgcgtga ggaaggcatg gtcgtcttcc aggcacttca
     6301 gcgcctcctc gagcgacgcg cacacgttcg gcaccttctt cgcctcttcc ggcggcaggt
     6361 ggtagaggtc cttgtccacc gggtcgccgg ggtggatctt gttctgcacg ccgtccaggc
     6421 ccgccatcag catcgcggca aaggcgagat atgcgttggc ggtcggatcc gggaagcgga
     6481 cctcgacgcg gcggccgctc gtgcccgagg cgtacggaat gcggcacgcg gccgaacggt
     6541 tgcgcgccga gtaggcgagg ttgatcggcg cctcgaagcc gggcaccagg cgcttgtacg
     6601 agttggtgcc ggggttggtg attgcgttca gcgacttcgc gtgcttgatg atgccgccga
     6661 tgtagtgcaa cgcgaagtca gagagcccgg cgtagccgtt gccagcgaag aggttcttgc
     6721 cgcctttcca caccgactgg tggcagtgca tcccggagcc gttatcgccg accacgggct
     6781 tcggcatgaa cgtcgccgtc ttgccgtagg aggccgccgt gttccacacc gtgtacttca
     6841 ggatctgcat ccagtcggcg cgctgaacga gcggcgcgaa gcgcgtgccg atctcgcact
     6901 gtccgggcgc cgccacctcg tggtgatgca cttcgacctc aacaccctgc tgctcgagcg
     6961 cgagcaccat ggcattgcgg atgtcgccca gcgtgtcgat cgggggcacc gggaaatagc
     7021 cgcccttgat cggggcgcga tgccccatgt tgcccgactc gtagtcgat
//
LOCUS       DASTPD010000108         8440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_261619, whole genome shotgun sequence.
ACCESSION   DASTPD010000108 DASTPD010000000
VERSION     DASTPD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261619"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1604
                     /locus_tag="VFR57_03800"
                     /pseudo
     CDS             1..1604
                     /locus_tag="VFR57_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434576.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            1783..2625
                     /locus_tag="VFR57_03805"
     CDS             1783..2625
                     /locus_tag="VFR57_03805"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU0258531.1"
                     /translation="MRCRLLASLAALFWAAGAPAHAELRPEWEFGLGATGLTLPDYRG
                     SDESRGYLLPFPYLIYRGEHFRVDRQGIRGIFFESDRVEINLSLNATPPVNSDDNRAR
                     QGMPHLDPTLEIGPRVDLRLLSDRAEEWALELRVPVRAAIATDFSHTKGIGFVLSPHL
                     SLTTRPLIAGVKWNFGAQAGPVFATREYHEYFYGVGPDVATPERPAFQASGGYSGTYA
                     LVSLSRRFPRFWVGAFTRYDMLSNAVFEDSPLVRRDHAFMAGIALAWVFAESSKKVEV
                     DVDE"
     gene            2720..2842
                     /locus_tag="VFR57_03810"
     CDS             2720..2842
                     /locus_tag="VFR57_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258532.1"
                     /translation="MERLQQWRRKQEGKLGYILLWALGVPIPVLFVIFLLRGCT"
     gene            2919..3134
                     /locus_tag="VFR57_03815"
     CDS             2919..3134
                     /locus_tag="VFR57_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEU0258533.1"
                     /translation="MAGLAACESLDKRGYSRQEQGTVGGAAAGGVIGNAATGGSTLGT
                     VGGAAVGGVIGNQAGQAYDERKEREGK"
     gene            3201..3953
                     /locus_tag="VFR57_03820"
     CDS             3201..3953
                     /locus_tag="VFR57_03820"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEU0258534.1"
                     /translation="MGVQEQQVPAAVERAMRVDLAAAFRLAVRLDLHEGVCNHFSVML
                     PDGKRFLLNRYGLHWSEVTASNLLALDAEGRILEGEGEFEKTAFYIHSRIHRANPRAT
                     CVLHTHMPYATSLTLLEGGRLEMVEQNALRFCDDIAYDDVYNGLVVDNAEGDRLARAL
                     GTKRVMFLANHGVIVVGPTLAEAFDSLYYLERACRLQVLARSMGGRLRPVRKEVVQET
                     FELIQADAPKYAGAHFEALKRILDREEPAYSR"
     gene            3943..4470
                     /locus_tag="VFR57_03825"
     CDS             3943..4470
                     /locus_tag="VFR57_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0258535.1"
                     /translation="MPAEVEVRPATPGDLPAIHSIYAHHVLHGLATFEEEPPSVQELE
                     RRFRDVTGRGLPYLVAEADGTVAGYGYCAPYRPRSAYRYALEDSIYVRPDMTGRGIGR
                     RLLEGLIRRCEGLGYRQMIAVIGDSANAGSIGVHAACGFLRVGTLRSAGFKFGRWVDS
                     VFMQRPLGPGDSTRP"
     gene            complement(4457..5434)
                     /locus_tag="VFR57_03830"
     CDS             complement(4457..5434)
                     /locus_tag="VFR57_03830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEU0258536.1"
                     /translation="MKAVLCKQFGPPDSLVLETLESPRAGAGEVVLSVKAASVNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKEVGAGVQGWRTGDRVMAFTTYGAFAEEVKTEA
                     SRLLPLPQNMDFVTGASFVLTYATTDHALRDRGALAAGETLLVLGAAGGVGLAAIEVG
                     MALRARVIACASSEDKLTVCRSHGADATINYATEDLRERIKALTAGHGPDVIYDPVGG
                     PYSEPAFRSIAWRGRHLVVGFAAGEIPKLPLNLALLKGASVVGVFWGDFARREPARFA
                     ESVKQLTKWYAEGRLKPHVSQTFPLEKAAEALKAMAARQVKGKVVLTVG"
     gene            5449..6339
                     /locus_tag="VFR57_03835"
     CDS             5449..6339
                     /locus_tag="VFR57_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0258537.1"
                     /translation="MPTLATDDGVKLYYEEAGSGAPVVFVHEFGGDYRSWEPQARYFS
                     RRYRCIAYNARGYPPSDVPEDFERYSQARARDDIRCVLDVLGIQRAHVVGLSMGAFAT
                     LHFGMTYGYRAMSITVAGGGYGSHPAQYREFQATSKKNAELIKRAGMAHFVETYGRGP
                     QRVQLEIKDPRGFEEYLRQFKEHSALGAINTLLGVQCRRPSFYDLTAEMARMDVPTLI
                     MSGDEEEPCLEVNLLMKRTIPTAALAILPRSGHAINLEEPALFNQLLEDFFHQVEAGR
                     WSARDARSTVPSVYGPGGKP"
     gene            6372..7121
                     /locus_tag="VFR57_03840"
     CDS             6372..7121
                     /locus_tag="VFR57_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0258538.1"
                     /translation="MGWITFNQPARRNAINGEMWRGIAPAMAQFDRDPEVRCIAFRGA
                     GTEAFSAGADISEFEEKRAARESVGAYDNLLDQVLHAIQDSPKPSVAMIYGFCLGGGL
                     EIALACDLRYCSASAQFGIPAARLGLAYNVEGHKRLLETVGHARAREIMFLGRRYNAD
                     EALAMGLVHQVLPEGDLEPFIAKILSDLSANAPLSILNSKTIIEQYVKAAGAPDHARM
                     QAAIERCAKSADYAEGRRAFMEKRKPQFRGR"
     gene            7118..8197
                     /locus_tag="VFR57_03845"
     CDS             7118..8197
                     /locus_tag="VFR57_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0258539.1"
                     /translation="MKIRAIRTRAVAAPMKRPLATSIATLTVAPLLLIDLETDAGIVG
                     RSYLFALAKHHLAPIAELVKAMAAMVEGDELAPLDLERKLRARYALLGVHNIVLFAMS
                     GIDMAAWDASAQELGQPLVRLLGAAPRPVPAYNSKGLGLLSERELTREASELVEEGFR
                     AIKLRLGRPSALEDLKALKAVKDQIGPQVTLMVDFNQGLTVAEAIRRGRMIDDEGGVY
                     WIEEPIRADDFAGCNRVRQEVRTPIQIGENFMGPEQMAQALAAGACDYVMPDAGRIGG
                     VTGWMRAAALAQAAGVEMSSHLFPEVSCHLLAATPSCHWLEYVDWADAVLQEPLTIKD
                     GYAVVPSAPGTGIHWNKAAVERYRA"
     gene            8197..>8440
                     /locus_tag="VFR57_03850"
     CDS             8197..>8440
                     /locus_tag="VFR57_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0258540.1"
                     /translation="MFTRHFAHWPPGQPRTLELPKESVYANLAVSAVKYPDRAAIDYY
                     GSRLSYAELKRQVDALAGCLQRRYGVARGDRVLLYAQ"
BASE COUNT         1476 a         2794 c         2794 g         1376 t
ORIGIN      
        1 gcatcctcgg ccgcacgctc accgtcgacc tgctgcaccc cgagacccag gacgtgctgt
       61 acccggcggg caccctgatc gacgaggaag gcgtggaagc gatcgacaac gtcggcatcg
      121 acgaggtcaa ggtgcgcacc gcgctgtcct gcgacacgcg ctggggcgtg tgctccaagt
      181 gctacgggcg cgacctcggc cgcggctcgc cggtgaacgt cggcgaggcg atcggcgtga
      241 tcgccgcgca gtcgatcggc gagccgggga cgcagctcac catgaggacg ttccacattg
      301 gcggcgcggc gtcgcggacc gcggtgcaga gccatgtcga ggcgaagtcg gccggaacgg
      361 tgcgtttcac gtccactatg cgctacgtct cgaacgcgaa gggcgacaag gtcgtgatca
      421 gccgctctgg cgaagtcacc atcgtggacg acaacggccg cgagcgcgag cgccacaagg
      481 tgccgtacgg cgcgcagctt ctcgcggctg acggcgacgc ggtgcgccct ggcaagcagc
      541 tcgccgcctg ggacccgcat caccggccga tcatcaccga gtacgccggg acggtgaagt
      601 tcgagcacgt ggaggagggc gccaccgtcg cgaagcagat cgacgacgtc accggcctct
      661 cgaccctcgt ggtcatcgac gccaagcggc gcaccaccgc cgccggcaag ggtcagcggc
      721 cgtcggtgaa gctcattaat gaggcgggag aagaggtgcg catcgccggc accgaccacg
      781 cggtcaacat ctccttcccg gtcggcgcgg tcatcgcggt gcgcgacggc cagcaggtgc
      841 aggtgggcga agtgcttgcc cgcatcccgc aggaggtgtc gaagacgcgc gacatcacgg
      901 gcggtctgcc gcgcgtggcc gagctcttcg aggcgcgctc gccgaaagac gcgggcatcc
      961 tggcggaagt caccggcacg gtgagcttcg gcaaggacac caagggcaag cagcgcctgg
     1021 tgatcaccga cctcgacaag caggagcacg agtacccgat cgcgaaggac aagcacctgc
     1081 tcgtgcacga cggccaggtg gtgaacaagg gcgagatgat cgtcgacggt tctgtggatc
     1141 cgcatgacct gctgcgcctg cttggcgtgg aggagctcgc gcgctacatc atcgacgagg
     1201 tgcaggacgt ctaccgcctg cagggcgtga aaatcaacga caagcacatc gaggtcatcg
     1261 tgcgccagat gctgcggcgc gtggagatca ccgacccggg cgacacgtcg ttcatcaccg
     1321 gcgagcaggc cgagcgcgcc gaagtgctcg aggagaacga gaaagccgaa aaggaagaca
     1381 agaagcctgc gcagtacgcc tacatgctgc tcggcatcac caaggcctcg ctctccaccg
     1441 actcgttcat ctcggcagcc tcgttccagg agacaacgcg cgtgctcacc gaagcggcga
     1501 tcatgggcaa gcgcgacgga ctgcgcggcc tgaaggagaa cgtcatcgtc gggcgcctga
     1561 tcccggccgg aaccggcctt gcgtaccaca acatccgcaa ggcgcaggcc gccgagccgg
     1621 tgctcgccga gattcctgca gccgctcccg aaacgccggc cgctgaaacc gcggccaccg
     1681 ccggcggcgg cagctgagga aaatcggggt cagagcccga ttatcctcgg atcgtcgcat
     1741 tcggcgatag cccgtgggat ttcggtgtcg gacaactagc ggttgcggtg ccggcttcta
     1801 gcgtcgctcg cggcgctgtt ctgggctgcg ggcgcgccag cgcatgccga gcttcgccct
     1861 gagtgggagt tcggcctcgg tgcgaccggg cttacgctgc ccgactaccg cggctctgac
     1921 gagagccgcg gctacctcct gccgtttccg tatctcatct accgcggcga gcactttcgc
     1981 gtcgaccggc agggtatacg cggcatcttc ttcgagtcgg accgcgttga gatcaacctc
     2041 agtctcaacg ccacccctcc ggtcaacagc gacgacaacc gtgcgcgcca gggcatgcct
     2101 cacctcgatc ccacgctgga gatcggccca cgcgtcgacc tcagactgct gagcgaccgc
     2161 gcggaggaat gggccctgga gctccgggtc ccggtgcgcg cggcaatcgc caccgacttt
     2221 tcgcacacga agggaatcgg cttcgtgctc tccccgcatt tgagcctcac cacgcggccg
     2281 ctgatcgcgg gagtgaaatg gaactttggc gcccaggcag gaccagtctt cgccacgcgc
     2341 gagtaccacg aatacttcta cggggtcggt ccggacgttg cgactccgga gcgccccgcg
     2401 ttccaggcga gcggcggtta ctcgggcacc tacgcgctcg tctcgcttag ccggcgcttt
     2461 ccgcgcttct gggtcggcgc cttcacgcgc tacgacatgc tgagcaatgc ggtgttcgag
     2521 gatagtcccc tcgtgcggcg cgaccacgcc ttcatggccg gcatcgcgct cgcctgggtc
     2581 ttcgccgagt cctcgaagaa ggtcgaggtc gatgtcgacg aatgatgtag tgaaatcccc
     2641 cacaatcgtc tgaacgaccg cggcacggcg cttgctgcca ctccgggcat ccgctcacaa
     2701 gagctcggag gttatggaca tggaaaggct gcaacagtgg cgccgcaagc aggaagggaa
     2761 gctcggctac atcctgctgt gggccctcgg ggttccgatc ccggtgctgt tcgtaatttt
     2821 cctgctgcgc ggctgcacct gagcgcgcat tcaattcatt tctcgaaaag gaggctattg
     2881 agatgaggaa gcttcttatt gcattcggca tcatctgcat ggcgggcttg gcggcttgcg
     2941 agtcgctcga caagcgaggg tactcgcgcc aggaacaggg caccgtgggc ggtgcggccg
     3001 cgggtggcgt gatcggaaat gcggccaccg gcggcagcac gctcggcacg gtcggcggcg
     3061 cggcggtcgg cggcgtgatc ggcaaccagg cgggccaagc atacgacgaa cgcaaggagc
     3121 gcgagggcaa gtaagtcggt acggcaatca agccgggaaa gggcgccctg acgggcgccc
     3181 tttcttttta taatggctct atgggagttc aagaacagca agtaccggcc gccgtcgagc
     3241 gcgcgatgcg agtcgatctc gccgccgctt tccgtctcgc cgtgcgcctc gacctgcacg
     3301 aaggggtctg caaccacttc agcgtcatgc tgcccgacgg caagcggttt cttctcaacc
     3361 gctacgggct gcactggagc gaggtgaccg catccaatct actggcgctg gacgcggaag
     3421 gcaggatcct tgagggcgag ggcgagtttg aaaagaccgc gttctacatt cactcgcgca
     3481 tccatcgcgc caatccgcgc gccacatgcg tgcttcacac ccacatgcct tatgcgacct
     3541 cgcttacgct gctcgagggc ggcaggctgg aaatggtcga gcagaacgca ctgcgctttt
     3601 gcgacgacat cgcctacgac gatgtctaca acggcctggt cgtggacaac gccgagggcg
     3661 accgtctggc gcgcgcgctg ggcacgaagc gcgtcatgtt cctcgcgaat cacggcgtga
     3721 tcgtggtcgg cccaacgctc gccgaggcgt tcgactcgct ttactacctg gagcgcgcct
     3781 gcaggctgca ggtgctcgcg cgctctatgg gcggcaggct gcggccggta aggaaagagg
     3841 ttgttcaaga aacttttgag ctcattcagg ccgatgcgcc gaaatacgcc ggcgcgcatt
     3901 tcgaggcgct gaagcgcatc ctcgaccgcg aggagccggc ttattcccgc tgaagtcgag
     3961 gtccggccgg caaccccggg cgatttgccg gccatccatt ccatctacgc ccatcatgtc
     4021 ctccacgggc tcgccacctt cgaggaagag ccgccttcgg tccaggagct cgagcgacgc
     4081 ttccgcgacg tgaccggccg cggccttccc tacctcgtcg cagaggcgga cggcacagtc
     4141 gccggttacg gctactgcgc gccgtatcgg ccgcggagcg cgtaccgtta cgcgctcgaa
     4201 gactccatct atgtgcgtcc agacatgacc ggccgcggca tcggtcgacg cctgctggaa
     4261 gggctcatcc ggcggtgtga agggctcggc taccgccaga tgatcgccgt catcggcgac
     4321 agcgccaacg cgggttccat cggcgtgcac gcggcttgcg gttttctccg tgtcggaacg
     4381 ctgcgctcgg cgggcttcaa gtttgggcgc tgggtggata gcgtgttcat gcagcgcccg
     4441 ctcgggccgg gcgactctac ccgaccgtaa gaaccacctt accctttacc tgcctcgcgg
     4501 ccatggcctt cagggcctcg gcagctttct ccagcgggaa tgtctgcgag acgtggggct
     4561 tgagccggcc ctcggcgtac cacttcgtca attgctttac gctctcggcg aagcgcgcgg
     4621 gctcacgccg cgcgaagtca ccccagaaaa cgccaaccac agacgcgccc ttgagaagcg
     4681 cgaggttgag cggcagcttg gggatctcgc ccgcggcgaa gccgaccacg aggtggcggc
     4741 cgcgccaggc gatcgagcgg aacgcgggct ccgagtaagg cccacccacc gggtcataga
     4801 tcacgtcggg gccgtggccc gcggttagcg ccttgatgcg ttcgcgcaga tcctcggtcg
     4861 cgtagttgat cgtggcgtct gcgccgtggc tgcggcacac cgtgagcttg tcctcgctcg
     4921 acgcgcaggc gatgacgcgc gcacggagcg ccatgcccac ctcgatcgcc gccagcccga
     4981 cgccgcccgc ggcgccgagc acaaggagcg tctctcccgc ggcgagcgcg ccgcggtcgc
     5041 gtagcgcgtg gtcggtggtc gcataggtga gaacgaacga cgcgccggtg acgaagtcca
     5101 tgttctgcgg caggggcagc agccgcgatg cctccgtctt tacttcctcg gcaaacgcgc
     5161 cgtaggtcgt gaacgccatg accctgtcgc ctgtgcgcca gccctggacg cccgcgccca
     5221 cctccttcac cacaccggcg agctcgctgc ccggcgagaa cggcagcggc ggcttgaact
     5281 ggtatttgtt ctggatgatg aggacatcgg gaaagttcac gctcgccgct ttcaccgaca
     5341 gcaccacctc gcccgcgcct gcgcgcggcg actccagcgt ctcgagcacc agcgaatcag
     5401 gcggtccgaa ctgtttgcag agcaccgctt tcatcgtagg cttgcattat gccaaccctc
     5461 gccacggatg acggcgtcaa gctctattac gaggaagcgg gcagcggcgc gccggtcgtg
     5521 ttcgtgcacg agttcggcgg cgactaccga agctgggagc cgcaggcgcg ctacttctcg
     5581 cgccggtacc gctgcatcgc ctacaacgcg cgcggctatc cgccctccga tgtccccgag
     5641 gacttcgagc gctactcgca ggcgcgcgca cgcgacgata tccgttgcgt gctggacgtc
     5701 ctcggcatcc agcgtgcgca cgtcgtcggg ctctccatgg gagcgttcgc cacgctgcac
     5761 ttcggcatga cgtacggata ccgcgcgatg tccatcacgg tggcgggcgg cggctacggc
     5821 tcgcaccccg cgcagtaccg cgagttccag gccacgtcga agaagaacgc cgagctcatc
     5881 aaacgcgcag gcatggcgca cttcgtcgaa acgtatgggc gcggcccgca gcgcgttcag
     5941 ctcgagatca aggacccgcg cggctttgaa gagtacctgc gccagttcaa agagcactcg
     6001 gcgcttggcg ccattaacac tcttctcggc gtgcagtgcc gccgcccgtc cttctacgac
     6061 ctgaccgccg aaatggcgcg catggacgtg ccgacgctca tcatgtcggg cgacgaagaa
     6121 gagccatgtc tcgaggtcaa cctcctcatg aagcgcacta tcccgaccgc ggcgctcgcg
     6181 atcctgccgc gcagcgggca cgccatcaat ctcgaggagc ccgcgctctt caaccagctg
     6241 ctcgaggatt tcttccacca ggtagaggcc ggccgctgga gtgcgcgcga cgcccgttcc
     6301 acggtgcctt ccgtctacgg cccgggaggc aaaccctgaa aggggaactc gtcaccgaaa
     6361 ggcgtgatgc cgtgggctgg atcaccttca accagccggc gcgccgcaac gccatcaacg
     6421 gcgagatgtg gcgcggcatc gcgcccgcga tggcgcagtt cgaccgcgac cccgaagtgc
     6481 gctgcatcgc gttccgcggc gcaggtacgg aggctttctc ggccggtgcc gacatctccg
     6541 aatttgaaga aaagagagct gcaagggaat cagtgggcgc gtacgacaac ctgctcgacc
     6601 aggtgctgca cgcgatccag gattcgccca agccttcggt cgcgatgatc tacggcttct
     6661 gcctgggcgg tggcctggag atcgcgctcg cctgcgacct gcgctattgc tcggcctcgg
     6721 cgcagttcgg catcccggcg gccaggctcg ggctcgccta caacgtcgaa ggccacaagc
     6781 gcctgctcga gaccgtcggc cacgcgcgcg cgcgggagat catgtttctc ggccggaggt
     6841 acaacgccga cgaggcgctc gcgatgggtc tggttcacca ggttcttccg gaaggggacc
     6901 tcgaaccttt cattgccaaa atcctttccg acctttcggc aaatgcgccg ctttcgattc
     6961 tcaactccaa gacgatcatc gagcagtacg tgaaggccgc aggcgcgccg gaccatgcgc
     7021 gcatgcaggc ggcgatcgag cgctgcgcaa agagcgcaga ttatgcagag ggacggcgcg
     7081 cgttcatgga gaagcgcaag ccgcagttcc ggggccgatg aagatccgcg ccatcaggac
     7141 acgcgcggtc gccgcgccga tgaagcggcc gctcgccacg agcatcgcga cgctaaccgt
     7201 cgcgccgctg ctgctcattg atctggagac cgacgcgggg atcgtcggcc gctcgtatct
     7261 cttcgcgctg gcaaagcacc atctcgctcc gatcgccgag ctggtcaagg cgatggcggc
     7321 aatggtcgaa ggcgacgagc tcgcgccttt ggacctcgag cgcaagctgc gcgcgcgcta
     7381 cgcgcttctc ggcgtgcaca atatcgtgct cttcgccatg tcaggcatcg acatggcggc
     7441 gtgggacgcg tctgcgcaag agcttggcca gccgctcgtg cgccttctcg gcgcagcgcc
     7501 gcgcccggtg cccgcctaca actccaaggg gctcggcctg ctttccgaga gggagctgac
     7561 aagagaagcg agcgagctcg tcgaagaagg gtttcgcgcg atcaagctgc ggctcggaag
     7621 gccctcggcg ctggaggatc tgaaggcgtt gaaagcggtg aaagaccaga tcgggccgca
     7681 ggtcaccctc atggtggact tcaaccaggg gctgaccgtg gccgaagcga tccgccgcgg
     7741 gcgcatgatc gacgacgaag gcggcgtcta ctggatcgag gaaccgatac gggccgatga
     7801 cttcgcgggc tgcaataggg tgaggcagga agtaagaacc cctattcaga tcggcgaaaa
     7861 cttcatgggg ccggagcaga tggcgcaggc gcttgccgcg ggcgcgtgcg actacgtgat
     7921 gcccgacgcc gggcgcatcg gaggtgtgac cggctggatg cgcgccgcgg cgcttgcaca
     7981 ggcggcgggc gtcgagatgt cgagccacct ttttcccgag gtgagctgcc acctgcttgc
     8041 cgcgacgccg agctgccact ggctcgagta cgtcgattgg gccgacgcgg tactgcagga
     8101 gccgctcacg atcaaggacg gttacgccgt cgttccgtcg gctccgggca ccggcatcca
     8161 ctggaacaaa gccgcggtcg agcgctaccg cgcctgatgt tcacgcgcca tttcgcccat
     8221 tggccgcccg gccagccgcg cactttggag ctgccgaaag agagcgtcta cgcgaacctc
     8281 gcggtgagcg cggtgaagta tcccgatcgc gcggcgatcg actactacgg cagccgcttg
     8341 agctatgcgg agctcaagcg ccaggtcgac gcgctcgccg gttgcctgca gcgccgctat
     8401 ggcgtggcgc gcggcgaccg tgtgctgctg tatgcgcaga
//
LOCUS       DASTPD010000109         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1078391, whole genome shotgun sequence.
ACCESSION   DASTPD010000109 DASTPD010000000
VERSION     DASTPD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078391"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFR57_03855"
     CDS             complement(<1..316)
                     /locus_tag="VFR57_03855"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEU0258541.1"
                     /translation="MRTAEVIRNTKETQIRVKVNLDGKGTARLSTGLPFLEHMLDQVA
                     RHGMLDLDIDAKGDLHIDAHHTVEDIGITLGQAVAKAIGDKAGVRRFGHAYVPLDEAL
                     SRV"
     gene            complement(330..1385)
                     /gene="hisC"
                     /locus_tag="VFR57_03860"
     CDS             complement(330..1385)
                     /gene="hisC"
                     /locus_tag="VFR57_03860"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0258542.1"
                     /translation="MTPEQLVRPEILALKAYHVAPAEGMVKLDAMENPYPLPAALRKE
                     LSEILARVDLNRYPDPAAPKLRALIARKMGVPSGMEILLGNGSDDLIQIVTLSLARPG
                     AVMMYPSPTFVMYAMNATYFGMRAVQTGLREDFSLNVDAFLKAMREQNPALIYIAYPN
                     NPTGVLYREEDVIAILKEAQGLVVLDEAYHVFARKTFMPRLAQFPNLVVMRTVSKLGL
                     AGIRLGYLAARPEWVEQFNKVRQVYNVNVLTQAAASFVLERLEVLEEQAARIRNERDH
                     LGKALAALKGVTVFPSAANFFLVRVPDADRTFEALKRQGVLVRNLNSSALRNCLRVTV
                     GTPDENRILLNALKEGL"
     gene            complement(1382..2644)
                     /gene="hisD"
                     /locus_tag="VFR57_03865"
     CDS             complement(1382..2644)
                     /gene="hisD"
                     /locus_tag="VFR57_03865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0258543.1"
                     /translation="MKLRRLSTRARGFDAKLAALTRYDAAQDPSVQKAVRKILADVRK
                     RGDAAVRAYTKKFDGVVPREYDVSGKFVDQIPAVQAEALRAAHDRIRAFHEKQLQQSW
                     DFTDVDGTRLGQQVRPLERVGLYVPGGKAAYPSSVLMNAVPAKVAGVRELVMASPNPS
                     PLVLAAAALAGVDRVLGIGGAQAIGALAYGTRSIPRVDKIVGPGNAYVAEAKRQVFGE
                     VGIDMVAGPSEILVIADGSANPDWVAMDLFSQAEHDESAQAILLSPDEAYLDAVAASI
                     GKLIGGMPRRAVIEASLKARGALILTRDLEEACAVANRIAPEHLELAVQDPEKILPKV
                     RTAGAIFLGQWSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIGVSERSA
                     AKLGRVAVTLAEGEGLSAHARAAQMRFE"
     gene            complement(2641..>2880)
                     /gene="hisG"
                     /locus_tag="VFR57_03870"
     CDS             complement(2641..>2880)
                     /gene="hisG"
                     /locus_tag="VFR57_03870"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029350.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU0258544.1"
                     /translation="VHVDLIRLYGSMELAPLVGLADAIVDLVSTGRTLKANGLVAVEE
                     IMPVSARLIVNQAALKTKRAALQPLIEAFAKAARR"
BASE COUNT          498 a          931 c          923 g          528 t
ORIGIN      
        1 ccacacgcga cagcgcctcg tcgagcggta cataggcgtg gccgaagcga cgcacgccgg
       61 ccttgtcgcc gatcgccttg gcaaccgcct ggcccagcgt aatgccgatg tcctccacgg
      121 tgtgatgggc gtcaatatgc aggtcaccct tcgcgtcgat gtcgaggtcc aacatgccgt
      181 ggcgcgcgac ctggtcgagc atgtgctcga gaaacggcag cccggtcgag agccgcgccg
      241 tgcccttccc gtccaggttt accttgaccc ggatctgagt ctccttggtg ttgcggatga
      301 cttcggcggt cctcattcca gcgccctcat tacaaccctt ccttcagggc gttgagcagt
      361 atacggtttt cgtccggcgt gccgaccgtg acgcgtaggc agttccgtag cgcggaactg
      421 ttcaggttgc gcacgaggac gccctggcgc ttgagcgcct cgaacgtccg gtcggcgtcc
      481 ggcacgcgaa cgaggaagaa gttcgccgcc gaaggaaaga cggtgacgcc cttcagcgcc
      541 gccagcgctt tgcccaaatg atctctttcg ttgcgaatgc gcgccgcctg ctcctcgagc
      601 acttcgagcc gttcaagcac gaagctcgcc gcggcctggg tcagcacgtt gacgttgtag
      661 acctggcgca ccttattgaa ctgctccacc cattccgggc gcgcggcgag gtacccgagg
      721 cgtatgccgg caagcccaag cttcgacacc gtgcgcatga cgacaaggtt gggaaactgc
      781 gcgaggcgcg gcatgaaagt cttccgcgcg aagacatggt acgcctcgtc caggacgaca
      841 aggccttgtg cttccttgag gattgcgatg acgtcctctt cccggtacag cacgccggtg
      901 ggattgttgg gataggctat gtagataagc gcggggttct gctcgcgcat cgctttcaga
      961 aaagcgtcca cattcagaga aaaatcctcg cgcagccccg tctgcaccgc ccgcatgccg
     1021 aagtacgtgg cgttcatcgc atacatcacg aacgtcgggc tgggatacat catcactgcg
     1081 cccggccgtg cgagggatag cgtgacgatc tgaatgagat cgtccgagcc gttgccgagc
     1141 aggatctcca tcccagaggg cacgcccatc ttgcgcgcga tcagcgcgcg cagcttcggc
     1201 gccgcggggt cgggatagcg gttgagatcg acgcgcgcga ggatttcaga cagttctttt
     1261 cgcaaggctg ccggaagcgg gtaggggttc tccatcgcgt cgagcttcac catgccttcc
     1321 gccggcgcga catggtaggc cttgagcgcc aggatctcgg gacggacgag ctgctccggg
     1381 gtcattcgaa cctcatttgc gccgcccttg cgtgcgcgct caggccttcg ccctcggcca
     1441 gggtcaccgc gactcgaccc agcttggcgg ccgatctctc ggagacgccg atcacactcg
     1501 tgcgtttctg gaagtcgtag accccgagcg gcgatgaaaa gcgcgccgtg cccgcggtcg
     1561 ggagtacatg gttggggccg gcgcaatagt cgccgatcgc ttccgagctc cattgaccga
     1621 ggaagatcgc gcctgcggtt cttactttgg gaagaatctt ttcagggtct tgcaccgcga
     1681 gctcgaggtg ctcgggtgcg atgcggttcg ccacagcgca cgcctcttcc aggtcgcgcg
     1741 tgagaatcag cgcgccgcgc gctttcagcg acgcttcaat caccgcgcgt ctgggcattc
     1801 caccgatcag ctttccgata gaggcggcaa ccgcgtcgag ataggcctca tccggagaaa
     1861 gcagaattgc ctgcgctgac tcgtcgtgct cggcctgcga aaaaaggtcc attgcgaccc
     1921 agtcggggtt cgccgagccg tcggcgatga cgaggatttc ggaggggccc gcgaccatgt
     1981 cgattccgac ctcgccgaac acctgccgct tcgcctcggc tacgtaggcg ttgcccggac
     2041 ccacgatctt gtcgacgcga gggatcgacc gagtgccgta ggcgagcgcg ccgatcgcct
     2101 gtgcgccgcc gatgccgagg acgcggtcaa cgccggcgag ggcggcggcc gcgagtacca
     2161 gcgggctggg attcgggctc gccatgacaa gctcgcgcac cccggccact ttcgccggca
     2221 ccgcgttcat caggaccgac gaaggatagg ccgcctttcc gcccggcaca tataacccaa
     2281 cgcgctcgag gggacgcacc tgctgaccga ggcgcgtacc gtcgacatcg gtgaagtccc
     2341 acgattgctg caattgcttc tcatggaaag cgcggatccg atcgtgcgcc gcccgcagtg
     2401 cttcggcctg aacggccggg atctggtcca cgaattttcc agagacatcg tattcacgag
     2461 gtacgacgcc atcgaacttc ttcgtatagg cacgtaccgc cgcgtcgccc cgcttgcgca
     2521 cgtcggcgag gatcttgcga accgccttct gcaccgacgg gtcctgcgcg gcgtcgtagc
     2581 gcgtgagcgc ggcaagcttt gcgtcgaagc cgcgggcgcg ggtggatagg cggcgcagct
     2641 tcaccggcga gccgcttttg caaaggcctc gataagcggc tgcaacgccg cgcgcttggt
     2701 cttgagcgcc gcctggttga cgatcagccg cgcggagacc ggcatgatct cttccacggc
     2761 gacgaggccg ttcgccttga gcgttctgcc cgttgagaca agatcgacga tggcgtcggc
     2821 gagcccgacg agcggcgcga gttccatcga cccgtagaga cgaatgaggt cgacgtgcac
//
LOCUS       DASTPD010000110         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1691298, whole genome shotgun sequence.
ACCESSION   DASTPD010000110 DASTPD010000000
VERSION     DASTPD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691298"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFR57_03875"
     CDS             complement(<1..678)
                     /locus_tag="VFR57_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU0258545.1"
                     /translation="MTSERTTYQSGFGNTFSSEAIPGALPVGRNSPQRPSHGLYAEVL
                     SGTAFTAPRAENQSTWLYKLRPSAMHGPYRRIDQGLVRSGPFVEVETPPNRLRWNPLP
                     MPKAATDFLEGLITVAGSGDAASQTGVAVHVYRANRSMGERYFWNADGELMFVPQQGA
                     LHLETELGRLDVEPGEIAVVPRGMKFKVEIEAAVRGYLCENYGASFRLPELGPIGSQG
                     LAQARDFQ"
     gene            722..1111
                     /locus_tag="VFR57_03880"
     CDS             722..1111
                     /locus_tag="VFR57_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258546.1"
                     /translation="MRITWGNRALTPIFFFSIAACSAIDHQRVEGWPELQIVEHRVPA
                     EEMVSRCQMYTGAASLPLACAEFKLAARRCDIWLSESFAPRAVVEHERLHCAGYDHVG
                     STNMKRFLEAHQGEGVTSAAAGASARR"
     gene            complement(1513..1914)
                     /locus_tag="VFR57_03885"
     CDS             complement(1513..1914)
                     /locus_tag="VFR57_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258547.1"
                     /translation="MLIAVTAWLAHLHVVAQIYHPVVRLSSPDGLVYTAVQDAKVERQ
                     ACGAANTRFLGPVKQGCKDCKVIAARCERQLEGIALAISEGKKLPHHQIRGPGLRMVI
                     AGPVEFARASCEHIVVEMRRNGLRGTCVHPS"
     gene            complement(1979..2647)
                     /locus_tag="VFR57_03890"
     CDS             complement(1979..2647)
                     /locus_tag="VFR57_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258548.1"
                     /translation="MIFNRALTPIFLFFSVCVQAQPCKVLDPELQGIYSGPCLNGLAE
                     GEGFAKGSSEYRGSFKAGRKNGRGVKTWPNGDRYEGEFLADRKEGYGSYVWGKGPWEG
                     ERYDGSFVGDRREGYGIYWWPNGDVYTGPWEADRATGPGSEMMRARAKHASEARAAVA
                     KRGQRVCREVAFGIGGRDWVRGEVADVEGEHVGVLIQESGRAALYPRGDVVWDVAQSW
                     TPCW"
BASE COUNT          496 a          943 c          858 g          473 t
ORIGIN      
        1 ctgaaaatcg cgcgcctgcg cgagtccctg cgagccgatc gggccgagct cgggaagacg
       61 aaacgacgcg ccgtagttct cgcaaaggta gccgcgcacc gccgcttcga tctcgacctt
      121 gaacttcatg ccgcgcggca cgaccgcaat ttcgcccggt tctacgtcga ggcgaccgag
      181 ctccgtttca agatggagag cgccctgctg cggcacgaac atgagctcgc cgtccgcgtt
      241 ccagaaatag cgctcgccca tgctgcgatt cgcgcgatag acatgaaccg cgacgccggt
      301 ctgcgacgcg gcgtcgcccg aacccgccac agtgattaat ccctcgagaa aatcggtcgc
      361 cgccttcggc atgggcaggg gattccaacg caggcggttc ggcggcgtct cgacttcgac
      421 gaacggcccg gagcgcacga ggccctggtc gatgcgccga tacggcccgt gcatcgccga
      481 gggccgcagc ttgtagagcc acgtgctctg gttctccgcg cgcggcgcgg tgaacgccgt
      541 accggaaagc acttcggcgt ataggccgtg cgagggacgc tggggcgagt tgcgaccgac
      601 gggcagcgcg ccgggaatcg cttcggacga gaaggtgttg ccgaaaccgc tctgataggt
      661 ggtccgctcg gaggtcatct gccccgaatt atccgcaatg cgccggccca agaatttgcc
      721 cgtgcgaatc acatggggaa atcgggctct gaccccgatt tttttcttct ccatcgcggc
      781 ctgttcggcg atcgatcacc agcgcgtcga gggctggccg gagctgcaga tcgtcgagca
      841 tcgcgtgccc gccgaagaga tggtctcgcg ctgccagatg tacaccggcg cggcatccct
      901 gccgctcgcg tgcgcggagt tcaagctggc cgcacgccgg tgcgacatct ggctctcgga
      961 gagtttcgca ccgcgcgccg tcgtcgagca cgagcggctg cactgcgcgg gctacgacca
     1021 cgtcggctcg accaacatga aacgctttct cgaagcgcat cagggcgagg gcgtcacttc
     1081 ggctgcagcc ggcgcgtccg cccgccggta gacactgggt gtgcggaaac gcaacacgta
     1141 cccaagaacg cgcctctccc ttgtccatgg gactaccagg gtcgaataac tgcgagtagg
     1201 ataatttgcg acacacgtgt agctaggtag ctactacgca ggagtcggaa atggtcaatc
     1261 gcaccaaatc ggccatcctc atgtccgcgc tcgccgcggt cttggtcttg gggactccaa
     1321 cctcatcctc gtcgagcgct gctaatgggg ggagcgcgca gccgctaccg gcgtgggtgg
     1381 tgccagcagc cgtcgcagcc gccgtcgtta cggtggttat cgtggcggcg aacgaagacc
     1441 cgctgttgca gacacccagc cactagacag gaccgacgag gggaaaggcc ccgctccggg
     1501 gcctttcttt tttcaggagg ggtggacgca ggtgccgcgc aggccgttgc ggcgcatctc
     1561 gaccacgatg tgctcgcagc tcgcgcgcgc gaattcgacg gggccggcga tcaccatgcg
     1621 cagtccgggg ccgcgaatct gatgatgcgg aagcttcttc ccctcggaga tcgcgagcgc
     1681 gatgccctcg agctggcgct cgcagcgagc ggcgatcacc ttgcagtcct tgcaaccctg
     1741 cttgaccggc ccgaggaagc gcgtgttggc cgcgccgcac gcttggcgct cgactttcgc
     1801 gtcctgcacg gcggtgtaca cgagaccatc gggcgaagac aggcgtacga caggatggta
     1861 gatctgcgcg acgacatgca gatgcgcgag ccatgccgtg acggctatga gcaccgcggc
     1921 aatcgccgtg tttcttacgg ctttcagtga atctcccttg cggagaattg taggagcctt
     1981 accaacacgg cgtccatgac tgggcgacgt cccacaccac gtcgccgcgc ggatagagtg
     2041 ccgcacggcc ggactcctgg ataagcacgc ccacgtgctc gccttcaaca tcagccacct
     2101 cgccgcgcac ccagtcgcgt ccaccgatgc cgaacgccac ctcgcggcac acgcgctgcc
     2161 cgcgcttcgc caccgcggca cgggcctcgg acgcgtgctt cgcgcgcgcg cgcatcattt
     2221 cgctgcccgg cccggtcgct ctatcggcct cccatggacc ggtatagaca tcgccgttgg
     2281 gccaccaata gatcccgtac ccctcgcgcc gatcgccgac gaacgagccg tcgtagcgct
     2341 cgccttccca tggccccttg ccccagacgt acgacccgta gccctccttc cggtcggcca
     2401 agaactcgcc ctcgtagcga tcgccgttcg gccaggtctt caccccacgg ccgttcttgc
     2461 gtcccgcctt gaagctccca cggtattcgg agctgccctt cgcgaacccc tcgccctcgg
     2521 ccagtccatt tagacacggc ccgctgtaga taccctgaag ctccggatcg agcaccttgc
     2581 acggctgcgc ctgaacgcaa accgagaaga acaagaaaat cggggtcaga gcccgattaa
     2641 agatcattga gctcccgcta ttaggggatt cgcggcccga gggccatatg ccagtatcca
     2701 actcgccctg ccactggcag gcgcaaacca tcaagaaggg gaggagtcac atgtccacac
     2761 gtctatgggt
//
LOCUS       DASTPD010000111         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_262921, whole genome shotgun sequence.
ACCESSION   DASTPD010000111 DASTPD010000000
VERSION     DASTPD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262921"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFR57_03895"
     CDS             complement(<1..415)
                     /locus_tag="VFR57_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0258549.1"
                     /translation="MNAPLTPVSFVSGNHSPLAARLRKEIDAEVMFDAASRARYSTDA
                     SIYQVEPIGVVVPRSEEAARAAIAIAVENGVPILPRGAGSSQCGQAVGAALVIDHTKY
                     LNKILEIGESHAVVQPGVVLDHLNAALRKHGLWFPV"
     gene            440..1639
                     /locus_tag="VFR57_03900"
     CDS             440..1639
                     /locus_tag="VFR57_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0258550.1"
                     /translation="MTRRAGRHFLQIPGPTNVPDRVLRAIDFPTIDHRGPEFATLGKE
                     VLAGMKRVFRTTQGEVVIYPASGTGAWEAALVNTLSPGDRVLMVETGHFATLWQKMAA
                     KLGLEVDFLPGDWRHGADPEAIEKRLRADSGKTLKALCVVHNETSTGVASRIADVRQA
                     LDAAKHPALFMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNCVSPAALA
                     ASKSAKLARSYWAWDEMITNGKSGYFPYTPGTNLLYGLREALKMLLDEEGLEAVFARH
                     QRHAEATRRAVRAWGLEIFALDPREYSASLTGVLMPAGHDADRVRKVILEAFDLSLGT
                     GLTKLGGKMFRIGHLGDFNDLMLMGTLAGVEMGLGLAGVPVKKEGVQAAMEYLAGCAQ
                     APARKVA"
     gene            1645..>2320
                     /locus_tag="VFR57_03905"
     CDS             1645..>2320
                     /locus_tag="VFR57_03905"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0258551.1"
                     /translation="MPGPLAGVKVLELAQIMAGPTCGIMLADLGADVIKVERIPGGDD
                     TRHMDRPSVRGESASFMAMNRNKRGIALNLKLPAAQEALKRMAARADVLTENYRKGTM
                     DKLGLGYETLKKVNPGLIYCSISGYGRTGPYADKGGYDLIAQGMSGLMSVTGEPGRAP
                     AKSGGPVCDINAGLLGALGVVSAYVHRLKTGEGQLVDTSLFEAGIQQMYWQAAIYFAT
                     GEPPGPT"
BASE COUNT          421 a          761 c          763 g          375 t
ORIGIN      
        1 ccaccggaaa ccacaggccg tgcttgcgca gcgcggcatt gagatggtcg agcaccacgc
       61 cgggttgcac gaccgcatgc gattctccga tttcgagtat cttgttcaag tacttcgtgt
      121 ggtcgatgac gagagccgca ccgaccgcct ggccgcattg cgagctgccc gcgccgcgcg
      181 ggaggatggg cacgccgttc tcaaccgcga tcgcgatcgc tgcgcgcgcg gcttcttcgc
      241 tgcgcggcac aacgaccccg atcggctcga cctggtaaat cgaggcatcg gtcgagtacc
      301 gagcgcggct cgcagcgtcg aacatcacct cggcgtcgat ctccttgcgc aagcgggccg
      361 ccaagggaga atgatttcca ctgacaaaac tgaccggagt caagggtgcg ttcatgcagg
      421 ccgtggttag aataacccaa tgactcgacg cgccggccgc cacttcctgc agatccccgg
      481 accgacgaac gtgcccgatc gcgtgctgcg cgcgatcgac ttcccgacca tcgatcatcg
      541 ggggcctgag tttgcgacgc tcggcaagga agtgcttgcc gggatgaagc gagttttcag
      601 gaccacgcaa ggcgaggtgg tgatctatcc cgcctcggga accggcgcct gggaggcggc
      661 gctcgtaaac acgctctcgc cgggcgaccg cgtgctcatg gtcgaaaccg gccatttcgc
      721 tacgctctgg cagaaaatgg cagcgaagct cgggctggaa gtcgacttcc tgcccggcga
      781 ctggcgccac ggcgcggatc ccgaggcgat cgagaagcgg ttgcgcgccg acagcgggaa
      841 gacgctgaag gcgttgtgcg tcgtgcacaa cgaaacttcg acgggcgttg cttcgcgcat
      901 tgcggacgtg aggcaagcgc tggacgctgc aaagcatccg gcgcttttca tggtcgacac
      961 gatctcctcg ctcgcctcga tagactatcg acacgacgaa tggggggtag acgtcaccgt
     1021 cgccggctca cagaaggggt tgatgctgcc gccgggtctt tcattcaact gcgtctcgcc
     1081 tgcagcgctc gctgcctcca agtcggcgaa actcgcgcga agctactggg cctgggacga
     1141 gatgatcacc aacggcaaga gcggctactt cccgtacacg cctggcacca atctgctcta
     1201 cggtctgcgc gaggcgctga agatgctgct cgatgaagag gggctcgaag cagtattcgc
     1261 gcgccaccag cgccacgccg aggcgacgcg ccgcgcggtg cgcgcctggg gccttgaaat
     1321 ctttgcgctc gacccgcgcg agtactccgc ctcgcttacc ggcgtgctga tgcccgccgg
     1381 gcacgacgcc gaccgcgtgc gcaaggtaat cctggaagcc ttcgatctct cgcttggcac
     1441 cgggctcacc aagctcggcg gcaagatgtt ccgcatcggc cacctaggcg acttcaacga
     1501 cctcatgctg atgggcacgc tcgccggggt ggaaatgggc ctcgggctcg cgggcgtgcc
     1561 agtgaagaag gaaggcgtgc aagcggcaat ggagtatctc gccggctgcg cgcaggcccc
     1621 ggcccgcaaa gtcgcgtagg gaaaatgccg ggcccgctcg ccggcgtgaa ggttctcgag
     1681 cttgcccaga tcatggcggg cccgacctgc ggcatcatgc tcgccgacct cggcgccgac
     1741 gtcatcaaag tcgagcgcat tcctggcggc gacgatacgc ggcacatgga ccggccctcg
     1801 gtgcgcggcg agtcggcgtc cttcatggcg atgaaccgca acaagcgcgg catcgcgctc
     1861 aacctcaagc taccggcggc gcaggaggcg ctcaagcgca tggccgcgcg cgcggacgtc
     1921 ctcaccgaga actaccgcaa gggcacgatg gacaagctcg gcctggggta cgagacactc
     1981 aagaaagtaa atccgggact gatctactgc tcgatctccg gctacgggcg taccggaccg
     2041 tacgccgaca agggcggcta tgatctcatc gcccagggaa tgtccggcct aatgtcggtc
     2101 accggcgagc ccggccgcgc gccggcgaag tcgggcggcc cggtgtgcga catcaacgcc
     2161 gggcttctcg gcgcgctcgg cgtggtttcg gcgtacgtgc accgcctgaa gaccggggaa
     2221 ggccagctcg tcgacacttc cctcttcgag gcaggtatcc agcagatgta ctggcaggcg
     2281 gcgatctact tcgcgaccgg cgagccgccc ggcccgaccg
//
LOCUS       DASTPD010000112         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_671795, whole genome shotgun sequence.
ACCESSION   DASTPD010000112 DASTPD010000000
VERSION     DASTPD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671795"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFR57_03910"
     CDS             <1..226
                     /locus_tag="VFR57_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258552.1"
                     /translation="VLLSGEGVQRRPLALDRLGDTFHYRAVGRGGELRDARTAQLTLA
                     GNDLKPYAPVHLAATGTWGSNVTPIFDTAI"
     gene            complement(276..1976)
                     /locus_tag="VFR57_03915"
     CDS             complement(276..1976)
                     /locus_tag="VFR57_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEU0258553.1"
                     /translation="MLLGGMYVREKQIGPYRYLYLVESVREGGRTKQRIIKNLGRKEH
                     VDANGDLDRLARSAARMAQRSMILSLLDQGGAPALATYRIGPPLAFERLWKETGCQVV
                     IEDLAAERGFGFSLERACFLTVLHRLMVSGSDRSADAWREDYRIEGTDDLALHHLYRA
                     MGWLGGELPDAEQTDRTPFAPRCLKDIVEERLFEARRDLFSELSVVFMDTTSLYFEGA
                     GGESLGQRGHSKDHRPDLMQMIVAVVMDQRGRPLCCEMWPGNTTDVAALIPVIDRLRR
                     RFGIGRVCVVADRGMISAQTLAALEARGLEYVLGVRERSAKEVRTLVLSDAAPFVPLV
                     IPRQGRADTELEAKAVTFDGRRYIVCRNRAEAKKDAADREAIVASLRRALRHGDKALV
                     GNRGYRRFLQAPEVRFAIDEARIVEDARFDGIFVLRTNTTLDPLQAMLRYRELQGVEQ
                     LFRSAKSLLATRPIFHKRDATIRGHVFCSFLALVLQKELRDRLAAAGLEFEWAEVLRD
                     LDRLQEVEVAQDGKRFILRTPTTGTAGKLFQTLRVALPPNLRDAAPEPDDTPAEPALL
                     "
BASE COUNT          375 a          773 c          734 g          360 t
ORIGIN      
        1 cgtcctgctg agcggcgaag gcgtgcagcg ccggccgctg gcgctggatc gtctgggcga
       61 cacctttcat taccgcgcgg tcggtcgcgg cggtgagctc cgggacgcgc gcaccgccca
      121 gctgacgctc gcgggcaacg acctcaagcc ctacgccccg gtgcatctgg ccgcaactgg
      181 cacctggggc tcgaacgtca cgccgatctt cgacaccgca atctgagaat gttcggcttt
      241 tcagggactt acggcgcgcg cagacggcct tagcactaca ggagggcggg ttcggcgggc
      301 gtgtcgtcgg gctcgggagc ggcgtcgcgc aggttgggcg gcagcgcgac gcgaagcgtc
      361 tggaacagtt tgccagcggt gcccgtggtc ggggtgcgga ggatgaagcg cttgccgtcc
      421 tgcgcgacct cgacctcctg aaggcgatcc agatcgcgca ggacctcagc ccattcgaat
      481 tcgagcccgg ccgcggcgag acgatcgcgg agctccttct gcagcaccaa tgcgaggaac
      541 gagcagaaca cgtggccgcg gatggtcgcg tcgcgcttgt ggaagattgg ccgggtcgcc
      601 agcagggatt tggcgctgcg aaacagctgc tcaacgccct gcagctcgcg atagcgcagc
      661 atcgcctgga gcgggtcgag ggtggtattg gtcctgagca cgaagatccc atcgaaccgc
      721 gcgtcctcga cgatccgagc ctcatcgatc gcaaagcgaa cctcgggcgc ctgcaagaag
      781 cgccggtagc cgcggttgcc gaccagcgcc ttgtcgccat ggcggagcgc tcggcgcagg
      841 cttgccacga tcgcctcccg gtcggcagcg tccttcttgg cttcggcccg gttgcggcag
      901 acgatgtagc ggcggccatc gaaggtcacc gccttggctt ccagctcggt atccgcccgt
      961 ccctgccgcg ggatgacgag cgggacgaag ggcgcggcgt cgctcagcac caaggtgcgg
     1021 acttccttcg cgctccgttc acgaaccccg agcacatact cgaggccacg cgcctcgaga
     1081 gccgcgagcg tctgagcgct gatcatcccg cgatcggcca ccacgcacac ccgtccgatc
     1141 ccgaagcgcc ggcgcaggcg gtcgatcacc gggatcagcg ccgcgacgtc ggtggtgttg
     1201 cccggccaca tctcgcagca cagcgggcgg ccgcgctgat ccatcaccac cgcgacgatc
     1261 atctgcatca ggtcgggtcg gtggtccttg gagtgacccc gctggccgag gctctcgccg
     1321 cctgccccct cgaagtacaa gctcgtggtg tccatgaaca ccaccgacag ctcactgaac
     1381 aggtcgcggc gggcttcgaa caaccgctcc tccacgatgt ccttgaggca gcgcggggcg
     1441 aacggcgtgc ggtctgtctg ctcggcgtcc ggcagctccc cgccgagcca gcccatcgcg
     1501 cggtagagat ggtggagagc gaggtcgtcg gtgccctcga tccgataatc ctcgcgccag
     1561 gcgtcggcgg aacggtcgga gcccgagacc atcagccggt gcagcacggt caggaagcag
     1621 gcgcgctcca gcgagaagcc gaagccgcgc tccgcggcca gatcttcgat caccacttgg
     1681 cagccggtct ccttccagag ccgttcgaag gcgagcggcg gaccgatccg gtaggtggcc
     1741 aacgccggcg cgccgccctg gtccaggagc gacaggatca tcgagcgctg cgccatgcgc
     1801 gcggccgagc gggccaggcg atcgaggtcg ccgttggcgt cgacgtgctc cttgcggccg
     1861 aggttcttga tgatccgctg cttcgttcgg ccaccctcgc gcacgctctc gaccaagtag
     1921 aggtagcggt aagggccgat ctgcttctca cggacgtaca tgccgccaag cagcacgcga
     1981 ttctaaaaac cgtcaattat aaagcgcctc ctagcataac tatttagcag cacgaccttt
     2041 cgccgccccc aaccccacct caacaaaacc aattagttag cgcgcaaaac tgcccgattc
     2101 cgtctgttca ctgtcgaaga tcggcctcac gtcgacctcg gccgggacgc gcacgcgcca
     2161 gcttgccggc ttggcctcgc cacgggcggt cgcgatggcg ttctcgaggc cagcctcatc
     2221 cttctcgaaa gcgcgcttgc tc
//
LOCUS       DASTPD010000113         6976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_263564, whole genome shotgun sequence.
ACCESSION   DASTPD010000113 DASTPD010000000
VERSION     DASTPD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263564"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..954
                     /locus_tag="VFR57_03920"
     CDS             391..954
                     /locus_tag="VFR57_03920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0258554.1"
                     /translation="MITRNEVNALPVRRYDGEVRIVGDDAQSVLADVLKEEVVGFDTE
                     TRPSFRPGESYPPSLAQVATANAVYLFQLRHAAAVQVVARMLEAPQVVKAGVALADDL
                     KALKKVREFSEKSIVDLGLAAKRQGLQQSGVRNLSALFLGFRIPKGTKTSNWARSQLT
                     AQQVAYAATDAWACRELYVRFRNLGFL"
     gene            988..1866
                     /locus_tag="VFR57_03925"
     CDS             988..1866
                     /locus_tag="VFR57_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0258555.1"
                     /translation="MEPGRETAVIFAELLGASELLGRADEAAAHAAVGEALASLEQVT
                     VASGARVLKRIGARLLVVTTDPDEAARTAVALQVAAHNLTGEHNFGLGVGFHFGPVIQ
                     DESDVFGDTVNTAARLVEKAARGQILLDAETADGITSLYRRSIRRLYQVQLKGLTEEI
                     SLCEIVWRADEPATFYPFDAAAPQPGRVKLKLKYRGEKLTLRRNSEALTIGRDPACGI
                     VVDGEHASRQHCVIARRNDKFVLSDKSTNGTFVTVEGEEELMVQRDEMTLRKQGWISF
                     GRPRGAGGDVVEFFCD"
     gene            1973..2203
                     /locus_tag="VFR57_03930"
     CDS             1973..2203
                     /locus_tag="VFR57_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258556.1"
                     /translation="MDLSTTIASTAIVLAVILTAGYAFGEAASEPTQEQTIEAREAER
                     KIAYEGDCETPNKTDEEHLACRDAYRPLETGL"
     gene            complement(2380..2667)
                     /locus_tag="VFR57_03935"
     CDS             complement(2380..2667)
                     /locus_tag="VFR57_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0258557.1"
                     /translation="MKRPVVYILASKPYGTLYIGVTSNLAARVEAHRNGSVDGFSKDY
                     GVHTLVYYELHADMYEAIQREKRLKKWNRAWKIRLIEEKNPEWKDLSGEAF"
     gene            complement(2818..4422)
                     /locus_tag="VFR57_03940"
     CDS             complement(2818..4422)
                     /locus_tag="VFR57_03940"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0258558.1"
                     /translation="MFEPEGFWRDLVSFTWNIRTCEGRGEIKAMLAARLGDVRPRNFQ
                     AKSAEWFSFETALGRGVGHLRLREDRCWTLLTTLQELKGHEEKAGPNRLKLPVPRAQG
                     LGIAEQPYVLIIGGGQGGIALAARLKRLGVPALVVEKNERAGDSWRKRYKSLCLHDPV
                     WYDHMPYIPFPNHWPVFSPKDKIADWLEMYTRVMELDYWTSTVCKRARFSEATNEWEV
                     LVMRDGQPHTLRPTHLVLATGMSGFPHVPQIPGADTFAGGIVHSSQYGAGAEWEGKQC
                     IVVGSGTSAHDICADLVESRAASVTMIQRAPTIVAKSETLMDLAWGPLYSEEALARGI
                     TTELADLSVASVPFKVLPELQRPIYAEMGKRDAELYSGLEKAGFQYNFGEDGSGIHSL
                     YLRRGAGYYIETGASQMIIERRIKVRNASVHSVDQRKVRLSDGSQLAADLIVFATGYG
                     SMNQWAEQLISKEVADRVGKVWGLGSDTKADPGPWEGELRNMWKPTQQTNLWFHGGNL
                     MQSRHYSLYLALQLKARFERMPTPVY"
     gene            complement(4554..5951)
                     /locus_tag="VFR57_03945"
     CDS             complement(4554..5951)
                     /locus_tag="VFR57_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0258559.1"
                     /translation="MKIEQFWDDSIVPALRDYIRIPAKSPHFDKAWQKNGYIDAAVEL
                     ASEWCRKNPVPGMKLEVVRLEGRTPVLLLDIPPFNSSSKETVLMYGHLDKQPEMVGWK
                     EGYGPWEPVIADGKLYGRGGADDGYAVFCAISALRELHEQKRPHARIVVLIECCEESG
                     SYDLPAYLDLLETRIGAPALVVGLDSGCGNYEQLWSTTSLRGLINGVLTVEVLTEGVH
                     SGDASGVVASSFRIARMLLDRIDDPATGIVKHAAFHAQIPPERAAQAKKAAQVLGEEI
                     WRKFPFVPNMQPMQADLADLVLNRTWRPFLAVTGADGLPPPASAGNVLRPKTELVLSL
                     RLPPTVKADSVAQQLKAILEAEPPYGARVSFKAGHAGTGWHAPQNAAWLEDAVNRASE
                     AHYKKPAMWMGEGGTIPFMAMLGLKYPAAQFLITGVLGPHSNAHGPNEFLHLDYAKRL
                     TACVADVLAAHASKT"
     gene            6038..6166
                     /locus_tag="VFR57_03950"
     CDS             6038..6166
                     /locus_tag="VFR57_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258560.1"
                     /translation="MQLSRATALIAVLLIVVLSAGYVRTEREASERPAPRALKNSR"
     gene            complement(6163..6603)
                     /locus_tag="VFR57_03955"
     CDS             complement(6163..6603)
                     /locus_tag="VFR57_03955"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEU0258561.1"
                     /translation="MRSAAWLRIATVLLAIILVASVTKWALTFSARRTPAEPLRTLPA
                     GQVEAKAPSVDSAAIARLLGASGSAGNIRALGVIAEGATGKGVALIGIEGQPARPVRA
                     GDAIAPGVVLAEVQKDGVVIDRSGARQQILIATKPAPQGILKAP"
     gene            6632..>6976
                     /gene="gspG"
                     /locus_tag="VFR57_03960"
     CDS             6632..>6976
                     /gene="gspG"
                     /locus_tag="VFR57_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEU0258562.1"
                     /translation="MRKRSPRGFTLVEIMVVVVIIGILAVLIVPRVLGRSDEARVAAA
                     KHDIAAISQSLKLYRLDNGRFPSTEQGLQALVTRPQSAPAPSNWRQYLDKLPNDPWGN
                     PYQYLNPGVNGEI"
BASE COUNT         1185 a         2252 c         2316 g         1223 t
ORIGIN      
        1 cgcggcgcac ggcgcgcgct ctttttcgag cagcgcatcg agccgcgggc tcggcacgcc
       61 gatcttgagg acgagctcgg gcttctcgtg gacgacgtag tcggagtcct ggtaggcggc
      121 gagcaggtcg ggatcgagag ccatacaaac gaaaggcccc gcgccgttcc tctgggcggg
      181 gccttgggtg cccgaactct cgggcggcgc ctagttgggc gcctgttacg tatcgcggcc
      241 gaagccgcgt gcgaggccgt aagggccagg tatcccgaca ggtggccggg aatcccgtgt
      301 gcttcgccgc gctaccatgg cgaccctctg cgggctcgcg ttcaatctat gactggcgcg
      361 tgccggcgtc aatcgcgatt cgaactactt ttgattacgc gcaatgaagt aaacgctctg
      421 ccggtgcgcc gctacgacgg cgaggtccgc atcgtgggcg acgatgcgca gagcgtgctc
      481 gccgatgttt tgaaagagga agtggtcggg ttcgacaccg agacgcggcc gtctttccgg
      541 ccgggcgaga gctatccacc gagcctcgcg caggtcgcga ccgcgaacgc ggtgtatctc
      601 ttccagcttc ggcatgccgc ggcggtgcaa gtcgtggcgc gcatgctcga agcgccgcag
      661 gtggtgaagg ccggagttgc gctcgccgac gacctgaaag cgttaaagaa ggtgcgcgag
      721 ttcagcgaga agtcgatcgt ggacctcggc ctcgcggcga agcgccaggg actccagcag
      781 tccggcgtgc gcaatttgtc cgcgcttttc ctcggtttcc gcatcccgaa gggcacgaag
      841 acctcgaact gggcgcgctc gcagctcacc gcccagcagg tggcatacgc ggcgaccgat
      901 gcgtgggcct gccgcgagct ctatgtgcgg tttcgcaatt tgggctttct ctagttatcg
      961 tatcgttcgg tccgggggaa taagaacatg gagccggggc gggaaacagc ggtgatcttt
     1021 gccgagctgc tcggcgcgag cgagctgctt ggccgcgccg acgaagcggc ggcgcacgcc
     1081 gcggtcggcg aggcgctcgc gagcctcgag caggtcacgg ttgcgagcgg cgcgcgcgtc
     1141 ttgaaacgca tcggcgcgcg ccttctcgtc gtcaccaccg accccgacga agcggcgcgc
     1201 accgcggtcg cgctgcaggt ggcggcgcac aacctcaccg gcgagcacaa cttcggcctc
     1261 ggcgtcggct tccacttcgg tccggtgatc caggacgaat ccgatgtgtt cggcgacacg
     1321 gtgaacacgg cggcgcgcct cgtcgagaag gccgctcgcg gccagatcct gctcgacgca
     1381 gagaccgccg acggcatcac ctcgctctac cggcgctcga tccggcgcct gtaccaggtg
     1441 cagctgaaag ggctgaccga agagatctcg ctttgcgaga tcgtctggcg cgccgacgag
     1501 ccggcgacct tttatccctt cgacgccgcc gcgccgcagc ccgggcgcgt gaagctgaag
     1561 ctcaagtacc ggggcgagaa gctcacgctg cgccgcaact ccgaggcgct cacgatcggg
     1621 cgcgatccgg cgtgcggcat cgtcgtcgac ggcgagcacg cctcgcgcca gcactgcgta
     1681 atcgcgcgca ggaacgacaa gttcgtgctc tccgacaaga gcacgaacgg caccttcgtc
     1741 accgtggagg gcgaggaaga gctgatggtc cagcgcgacg agatgacgct tcgcaagcag
     1801 ggctggatct cgttcggccg tccgcgcggc gcgggcggcg acgttgtgga atttttctgc
     1861 gactagtcga aattcttcgt caggcccgtg acgggcgtga cggactccca agacgttttt
     1921 tgtaaaccct gccacagtgc tcctcgtaga acgttagcga ggggagccgt aaatggattt
     1981 atcaacgacg atcgcatcga cggcgatcgt tcttgcagtg atcctcaccg caggttatgc
     2041 cttcggcgaa gcagccagcg agccgaccca ggagcagaca atcgaagcgc gcgaagcgga
     2101 acgcaagatc gcctacgagg gcgattgcga gacaccgaac aagaccgacg aagaacacct
     2161 agcttgccgg gatgcgtacc ggccgctcga aaccgggttg taggcagggc tgcgtaaggg
     2221 aggttcgggg tcggggccca aagcctcgac cctttttttg tgccgtcatc cccgcgcagg
     2281 cggggaccca gttggtttta agccgtcatc cccgcgcagg cggggacccc agttggtttt
     2341 aagccgtcat ccccgcgcaa gcggggaccc agtcagactc tagaacgcct ccccagacag
     2401 atccttccac tccggattct tctcctcgat cagtctgatc ttccaagcgc ggttccactt
     2461 tttcagtctc ttctctcttt gaatcgcctc gtacatgtca gcatgcagct cgtagtacac
     2521 gagcgtatgc acaccgtaat ccttgctgaa cccgtccacg gaaccgttgc gatgcgcttc
     2581 gacgcgagcg gcaagattgc ttgtcacgcc gatgtagagc gttccgtagg gtttgctcgc
     2641 gaggatgtag acaactggac gcttcacgcc gcgagcaacg cgggtgggca ggctcacgtt
     2701 gacggaaaac aaattggatc cccgccttcg cggggaggac ggcctaattg ggtccccgcc
     2761 ttcgcgggga ggacggccta attggatccc cgccttcgcg gggacgacga gtgaaaccta
     2821 atagaccggc gtcggcatcc tttcgaagcg cgctttcagc tgcagcgcga ggtagagcga
     2881 gtagtggcgc gactgcatca gattcccgcc gtggaaccag aggttcgtct gctgcgtggg
     2941 cttccacatg ttgcgcagct cgccctccca cgggccggga tcggccttgg tgtcggagcc
     3001 tagaccccag accttgccga ctcggtcggc gacttccttg ctgatcagct gctcggccca
     3061 ctggttcatc gagccgtagc cggtggcgaa gacgatgagg tcggcggcga gctgcgagcc
     3121 gtcggagagg cggaccttgc gctggtcgac cgaatgcacg gatgcgtttc gcactttgat
     3181 gcggcgctcg atgatcatct gcgaggcgcc ggtctcgatg tagtagcccg cgccgcgccg
     3241 gagatacagc gaatggatgc ccgagccgtc ctcgccgaag ttgtactgga agccggcttt
     3301 ctcgaggcct gagtagagct cggcgtcgcg cttgcccatc tcggcataga tcgggcgctg
     3361 aagctcgggg agaaccttga aaggcaccga ggcgacgctc aggtcggcga gctcggtggt
     3421 gatgccgcgc gcgagcgcct cttccgagta gagcggtccc caggcgaggt ccatgagcgt
     3481 ctcggatttc gcgacgatgg tcggcgcgcg ctggatcatg gtgacagagg ccgcgcggct
     3541 ttcgacgaga tccgcgcaga tgtcgtgggc cgaggtgccg gagccgacca cgatgcactg
     3601 ctttccctcc cattcggctc ccgcgccgta ctggctggag tggacgatac cgccggcgaa
     3661 cgtgtcggcg cccggtattt gcggcacatg cggaaaaccg gacatgcccg tggcgagcac
     3721 gaggtgggtg gggcggagcg tgtgcggctg gccgtcgcgc attacgagca cttcccattc
     3781 gttcgtggct tcgctgaagc gcgcgcgttt gcagaccgtg gaggtccagt aatcgagctc
     3841 catcacgcgc gtgtacatct cgagccagtc ggcgatcttg tccttgggcg agaacaccgg
     3901 ccaatggttg ggaaaaggga tgtacggcat gtggtcgtac cagaccgggt cgtgcaggca
     3961 gagcgacttg tagcgcttcc tccacgaatc gccggcgcgc tcgttcttct cgaccacgag
     4021 cgccggcacg ccgaggcgct tcagccgcgc cgcgagcgcg atgccgccct gtccgcctcc
     4081 gatgatcagc acatacggct gctcggctat tcccaggccc tgcgcgcgcg gcacggggag
     4141 cttcagtcgg ttagggccgg ccttttcttc gtggcctttc agctcctgga gcgtcgtgag
     4201 cagcgtccag caccggtctt ccctaaggcg cagatgcccg acgccgcgcc cgagcgcggt
     4261 ttcgaaactg aaccactcgg cgctcttcgc ctggaagttg cgcgggcgca catcgccgag
     4321 ccgcgcggcc agcatcgcct tgatctcgcc gcggccttcg caggtcctga tattccaggt
     4381 gaacgaaacg agatcgcgcc agaacccttc gggctcgaac atgccaaccg cgccattgat
     4441 gtcgccgcgc tcgagcgccg cgccgaactt ctgcagccag gccgaattcg gggtcagggt
     4501 gcgaattccg taaggtatta gcaggcgtta cgaattcgca ccctgacccc gaattacgtt
     4561 ttggaggcgt gagcggccaa tacgtcggct acgcaggcgg tgaggcgctt ggcgtagtcg
     4621 aggtgcagga attcgttggg gccgtgggcg ttggagtgcg ggccgaggac gccggtgatg
     4681 aggaactgcg ccgccgggta tttcaggccg agcatcgcca tgaacgggat ggtgccgccc
     4741 tcgcccatcc acatggcggg tttcttgtag tgcgcctcgg acgcccggtt tacggcgtcc
     4801 tctaaccagg cggcgttctg cggcgcgtgc cagccggtgc cggcgtggcc ggccttgaag
     4861 ctgacgcgcg cgccgtaagg cggctcggcc tcgaggatcg ccttcaattg ctgcgcgacg
     4921 ctgtcggctt tcactgtggg cgggagtcga agcgagagca cgagctcggt cttcggcctc
     4981 agcacgttgc ccgcgctcgc cggcggcggc aggccgtcgg cgccggtaac ggcgaggaac
     5041 ggccgccagg tacggttaag gacgaggtcg gccaagtcgg cctgcatcgg ctgcatgttt
     5101 ggcacgaacg ggaacttgcg ccagatctcc tcgccgagca cttgcgccgc cttcttcgcc
     5161 tgcgcggcgc gctccggcgg gatctgcgcg tggaaggccg cgtgcttcac gatccccgtg
     5221 gctggatcgt cgatgcgatc gagcagcatg cgagcaatgc ggaagctgga cgccaccacg
     5281 ccgctcgcgt cgcccgagtg cacgccttcg gtcagtacct cgaccgtgag cacgccgttg
     5341 atcagcccgc gcagcgaggt cgtgctccag agctgctcgt agttgccgca gcccgaatcg
     5401 agacccacaa ccagggcggg cgcgccgatg cgggtttcca ggagatcgag ataagccggg
     5461 aggtcgtagc tgccgctctc ttcgcagcac tcgatcaaaa cgacgatgcg cgcatgcggc
     5521 cgcttctgct catgcaactc tctaagagcc gaaattgcac aaaaaacggc gtagccatca
     5581 tcggccccgc cgcggccata gagcttgccg tccgcgatca ccggctccca cggcccgtag
     5641 ccttccttcc agccgaccat ctcgggctgc ttatccaggt gcccgtacat caggacggtt
     5701 tctttcgacg aggagttgaa cggagggata tccaagagca aaaccggcgt gcggccttcg
     5761 aggcgcacga cctcgagctt catgccgggc accggattct tgcggcacca ctcactggcg
     5821 agctcgaccg cggcgtcgat gtacccgttt ttctgccacg ccttgtcgaa gtgcggcgac
     5881 ttggccggga tgcggatgta gtcgcgaagc gcgggaacga tcgaatcgtc ccagaactgc
     5941 tcgattttca tgaaggcctc aatctatcga caccccggac ggactgcatg gactaagcga
     6001 aacacgcaat agcggccgag cacaattcga acatcccatg cagctctctc gcgccacagc
     6061 gctgatcgcg gtgttgctca tcgtcgtgct ctcggccggc tacgtccgca ccgagcgcga
     6121 ggcgagcgag cgcccggcgc cgcgcgcact caagaactcc cgctaaggcg ccttcaggat
     6181 tccctgcggc gcaggcttgg tcgcgatgag gatctgctgg cgcgcacccg agcggtcgat
     6241 caccacgccg tccttctgaa cctcggcgag cacgactccg ggtgcgatcg cgtcgccggc
     6301 gcgcaccggg cgcgcgggtt gtccttcgat gccgatcagc gccacgccct tgccggtcgc
     6361 gccctcggcg atcacgccaa gcgcgcggat attgccggcc gaacctgagg ctccgagtag
     6421 acgggcgatg gcggcgctat cgaccgaagg cgccttggcc tccacctggc ccgccggcag
     6481 cgtgcgcagc ggctcggccg gcgtgcgccg cgcgctgaag gtgagtgccc atttcgtcac
     6541 gctcgccacc agaatgatgg caaggagcac cgtcgcgatg cgaagccagg ccgcagagcg
     6601 catgcgccat tgtcctgcat aatcggcgga catgagaaaa cgaagtcccc ggggcttcac
     6661 cctcgtcgag atcatggtgg tggtggtgat catcggcatc ctcgcggtgc tgatcgtgcc
     6721 gcgggtgctc ggccgcagcg acgaggcgcg cgtcgcggcc gccaagcacg acatcgcggc
     6781 gatctcgcag tcgctcaagc tctaccgcct cgacaacggc cgcttcccgt cgaccgagca
     6841 gggcctgcag gcgctcgtga ccaggccgca gtcggcgccg gcgccgtcga actggcgcca
     6901 gtacctcgac aagctgccga acgatccctg gggcaacccc taccagtacc tgaacccggg
     6961 cgtgaacggc gagatc
//
LOCUS       DASTPD010000114         8390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_672709, whole genome shotgun sequence.
ACCESSION   DASTPD010000114 DASTPD010000000
VERSION     DASTPD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672709"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /gene="metH"
                     /locus_tag="VFR57_03965"
     CDS             complement(<1..2007)
                     /gene="metH"
                     /locus_tag="VFR57_03965"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU0258563.1"
                     /translation="MDKIEALLREKILLLDGAMGTMIQRARLAEEDYRGARFRDWPRE
                     LRGNNDLLSLTQPRLVRDIHSQYLQAGADIIETNTFNSTAIALADYGMESLAREFNRA
                     AARLATEAAREWKARTPDQPRFVAGVLGPTNKTASISPDVNDPGFRAVTFDELVTAYC
                     DAIAGLLEGGVDLLLVETVFDTLNAKAALFAIEHTFAARSQRLPVIVSGTITDASGRT
                     LSGQTAEAFYNSVRHARPLAVGLNCALGAKELRPYVEELAAIAESYVSCHPNAGLPNA
                     FGEYEDTPESMAAHAAEWARAGWLNLAGGCCGTTPEHIRAIGEALRPLTPRRIPERAR
                     ALRLSGLEPLNVDDTSLFVNIGERTNVTGSRAFARLVLGGDYAGALAVARQQVENGAQ
                     VIDVNMDEAMLDSEKAMATFLRLVAAEPDIARVPVMIDSSKWSVIEAGLKCVQGKPIV
                     NSISLKEGESEFLRQAALCRRYGAAVVVMAFNEQGQADTLERRIAVCRRAYELLVHKA
                     GFQPADIVFDPNIFAVATGLEEHARYAIDYIEAVRWIRANLPHAKVSGGVSNLSFSFR
                     GNDPVREAMHTAFLYHAIRAGLTMGIVNAGQLGVYEQIEPDLRQRVEDVLFDRRADAT
                     ERLVEFAATVKAGARESAKDEAWRSASVESRLAHALVNGISSHIV"
     regulatory      complement(2044..2121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2168..2599
                     /locus_tag="VFR57_03970"
     CDS             2168..2599
                     /locus_tag="VFR57_03970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU0258564.1"
                     /translation="MSEFASIVGHFPCQVHWGDCDPAGIIFYPTYFRWMDAATWALMG
                     SVGYGPKRMREQHLAMPLVAADCQFVAPAQHGDRCEVRSRITRFGGASFVVAHDIVRD
                     DGTTLAQGSEKRVWGRYASGPGTPLKGEHIPEEVRALFRAR"
     gene            complement(2574..3950)
                     /gene="pcaB"
                     /locus_tag="VFR57_03975"
     CDS             complement(2574..3950)
                     /gene="pcaB"
                     /locus_tag="VFR57_03975"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEU0258565.1"
                     /translation="MGCGPAGQERDGVLRFLSLELVFSDPHTAEALSDEKLLAAMARF
                     EAALARASTGLVPLEHAETIARACENASFDVARLANEARHAGTLAIPFVKELTAQVPG
                     EAARYVHFGATSQDVIDTAVVVCLKEAAHRVLVSARRLGDAAARLADRHRDTPTVART
                     LLQPATPVPFGWKAAVWLSLVANSYRQFRDAAEEVCVLQFGGPAGTLAAFGSDGELVS
                     TRLAERLGLRKPPIPWHSARDRFARFGAEAAILVGAAGKIARDVSLLMQPEVGEAFEP
                     ASRGRGGSSALPHKRNPAACVLALEASQRAPALAASLMANLVPEHERGLGQWQAQWLT
                     LRSLVCATASGVAAMAEAIERLEVRTDTMAANLERTQGLVFSEAVSVRLAQTLGKSAA
                     HTLTEKLCARAVAEKKHLREVLLAEDDVTRIIPAGELEALFEAKRAFGSAKPMIDRVL
                     AIWRETAP"
     gene            complement(3899..4462)
                     /gene="pcaG"
                     /locus_tag="VFR57_03980"
     CDS             complement(3899..4462)
                     /gene="pcaG"
                     /locus_tag="VFR57_03980"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEU0258566.1"
                     /translation="MSLRATTSQTIGPYLRIGLEWMVIEDLAPQGVAGEHVRIEGRVL
                     DGDGRPVNDAAVEIWQANSRGKYASPEDAQAKPIEQSFRGYGRSLTDDGGAFRFRTIK
                     PGRVPGPDRKLQAPHITVTIFMRGLLKQLQTRIYFPDDPANADDPVLNLIPAERRGTL
                     IAKRRADGEFEWDVVLQGKNETVFFDF"
     gene            complement(4459..5169)
                     /gene="pcaH"
                     /locus_tag="VFR57_03985"
     CDS             complement(4459..5169)
                     /gene="pcaH"
                     /locus_tag="VFR57_03985"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEU0258567.1"
                     /translation="MTDPAPYRRPYRDTQPPYLYPPYASTIRRAPTQPLVALAHTLSE
                     LTAPIFGYGDIKPTDNDLTQQHEGEPLGERLVVSGRVLDENSRPLPGILIEIWQANAA
                     GRYNHRVDQHDAPLDPNFTGAGRVLTDTEGRYRFMTIRPGEYPWRNHYNAWRPAHIHF
                     SLFGPAIVQRLITQMYFPGDPLLEYDPMFTCVPDQKARNRLISIFDWETTQPEFALGY
                     RFDIVLRGREATPMENRP"
     gene            complement(5174..5809)
                     /gene="maiA"
                     /locus_tag="VFR57_03990"
     CDS             complement(5174..5809)
                     /gene="maiA"
                     /locus_tag="VFR57_03990"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEU0258568.1"
                     /translation="MKLYTYFRSSAAFRVRIALNLKGLAYEPVFIHLAKGEHRKSEYA
                     GKYPQGLLPTLLTDGLELSQSLAIIEYLDETHPNPPLLPRDAPGRARVRSLALLVACE
                     IHPLNNLRTLQYLRRELGHNEEQVNAWYRHWIADGLAKLEADLLRSKKSKCCHGDTPT
                     MADCCLVPQVFNAKRYNCDLASYPATMRVFEECMKLEAFERAQPAKQPDAE"
     gene            complement(5806..6498)
                     /locus_tag="VFR57_03995"
     CDS             complement(5806..6498)
                     /locus_tag="VFR57_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0258569.1"
                     /translation="MGYAIPLWTPPVLPVAGTADSFPIRRIFCVGRNYAEHQKEMGGD
                     GREQPFFFIKSAHALVPGGGEVRYPAKTSNYHYETELVVALAKGGRRIDAKRGNDHVF
                     GYAVGLDMTRRDLQQWGKDHGRPWDFGKDFDQAAPCGALVPAAKIGHPGRGAIWLTVN
                     GKQRQRADLGDMIWSVPEQIAYLSEYYTLEPGDLIYAGTPAGVGPVKPGDELHAGIDG
                     VGELKVKILPPL"
     gene            complement(6523..7389)
                     /locus_tag="VFR57_04000"
     CDS             complement(6523..7389)
                     /locus_tag="VFR57_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0258570.1"
                     /translation="MKICRFDDDRLGLVEGSEVLDVTEALATIPPPKWPYRHGDALIT
                     HLPAVLEKAKALAGRAARRPLFAVRLRPPVANPSKIVNAPINYQAHIDEAKRDQGIAH
                     GREIKTIADWGLFLKSPSSLAGAGDGIKLRFRERRNDHEVELAVVIGREANRVDRATA
                     LDYVCGYSIGLDMTLRGPELPSFRKSIDTYSVLAPWLVTRDEVPDPGNLELWLTVNGE
                     PRQRSNTSRMVYDVPRLIEYATSFYTLYPGDLIFSGTPEGVGPVKPGDEIRAGIERIG
                     EFAIRVAPDYAG"
     gene            7535..7966
                     /locus_tag="VFR57_04005"
     CDS             7535..7966
                     /locus_tag="VFR57_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0258571.1"
                     /translation="MEPKQTLRPGLLPGLLGYRLRLAQQAVFRDFGESVSDLPPGRVG
                     ILLIIDANPGVTQTRLAQAVGLDRSTLVGVLDALEARGLVERRRGKDRRTNGLLLTRA
                     GRARVTELKRRIRVHERRVAARLTAEEKTQLLALLSKLAGR"
     gene            complement(8006..>8390)
                     /locus_tag="VFR57_04010"
     CDS             complement(8006..>8390)
                     /locus_tag="VFR57_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEU0258572.1"
                     /translation="GLAAGLVCLWGVSGLKKLLGADDSLDVFGVHAVGGILGALLTGV
                     FNSPDLGGPGFVTDWVTAKTGFSSISEQLWVQAKAVGLTILWSGIVAAIAYKLVDLTI
                     GLRVTEEDEREGLDTAAHGEAAYRM"
BASE COUNT         1409 a         2696 c         2781 g         1504 t
ORIGIN      
        1 aacgatgtgg ctggagatgc cattcaccag ggcatgcgcc aggcggctct cgacgctcgc
       61 cgagcgccac gcctcgtcct tggcgctttc ccgggcgccg gccttgacgg tcgccgcaaa
      121 ctcgaccagc cgttcggtcg catccgctcg gcggtcgaac agcacgtcct ccactcgctg
      181 ccgcagatcg ggctcgatct gctcgtagac gccgagctgt ccggcattca cgatgcccat
      241 cgtcaggccc gcgcggatcg cgtggtagag gaacgcggtg tgcatggcct cgcgcaccgg
      301 gtcgttgccg cggaacgaga acgagaggtt cgacacgccg cccgagacct tggcgtgcgg
      361 caggttggcg cggatccagc gcaccgcttc gatgtagtcg attgcgtacc gcgcatgctc
      421 ttcgaggccg gtggcgaccg cgaagatgtt cggatcgaac acgatatccg cgggttgaaa
      481 tcccgcctta tggacaagca gctcataagc gcgccggcag accgcgatgc gccgctccag
      541 cgtatcggct tggccttgct cgttgaacgc catgaccacc acagcggccc cgtaacgtcg
      601 gcaaagcgcc gcctggcgca ggaactcgct ttcgccctcc ttgagcgaga tcgagttgac
      661 gatcggcttg ccctgcacgc atttaaggcc ggcctcgatc acgctccact tggagctatc
      721 gatcatcacc ggcacgcgcg cgatgtcggg ctcggccgcg accagccgca ggaaggtcgc
      781 cattgccttc tcggagtcga gcatcgcttc gtccatgttg acgtcgatca cctgcgcgcc
      841 gttctccacc tgctgccgcg cgactgcgag cgcgccagcg taatcgccgc cgagcacgag
      901 tcgcgcgaag gcacgcgagc cggtgacgtt ggtgcgctcg ccgatgttga cgaagagcga
      961 tgtgtcatcc acgttgagcg gctccagacc cgagaggcgt agtgcgcgcg ctcgctcagg
     1021 aatgcgacgc ggggtgagcg gacgaagagc ctcgccgatc gccctgatgt gctcgggcgt
     1081 ggtgccgcag cagccgccgg ccaggttgag ccagccggcg cgcgcccatt ccgcggcgtg
     1141 ggcagccatc gattccggcg tgtcctcgta ctcgccgaag gcattgggca ggcccgcgtt
     1201 cggatggcac gagacatagc tttccgcgat cgcggcgagc tcctcgacat agggcctgag
     1261 ctccttagcg cccagcgcgc agttgagacc cacggcaagc ggccgtgcat ggcgcacaga
     1321 gttatagaac gcctcggcgg tctggccaga cagcgtgcgt cccgacgcgt cggtgatcgt
     1381 acccgatacg atcaccggca agcgctgcga ccgcgccgcg aatgtgtgct cgatggcgaa
     1441 tagcgccgcc ttggcattca gggtgtcgaa caccgtctcg accagcagca ggtcgactcc
     1501 gccttcgagc aatccggcga tcgcgtcgca gtacgcggtc accagctcat cgaatgtcac
     1561 cgcgcggaag ccggggtcgt tcacgtcggg cgaaatcgag gcggttttgt tggtggggcc
     1621 gagcacgccg gcgacaaagc gaggctgatc cggcgtgcgc gctttccatt cgcgcgcggc
     1681 ctcggtcgcg agccgagccg cggcgcggtt gaactcgcgc gcaagcgact ccatgccgta
     1741 gtcggcgagc gcgatcgcgg tggagttgaa ggtgttggtc tcgatgatgt cggcgccggc
     1801 ctgcaggtac tggctgtgga tgtcgcgcac gagccgcggt tgtgtaagcg acagcaggtc
     1861 gttattgccg cgcagctcgc gcggccaatc gcggaaacgc gcgccgcggt agtcttcctc
     1921 cgcgagccgc gcgcgctgga tcatggtgcc catggccccg tcgagcagga gaatcttctc
     1981 gcgcaggagc gcttcgatct tatccatgtc tgaaaacgaa aaagcccgct cggcttgcag
     2041 agaacgggct tcgggctcgc ctctttagcg gtatttgtaa cgcgcccgca agctgagcat
     2101 caaatcggcg cggcgcgtgc gattctgccc atcaagtcct gatgtgtcaa cggcttataa
     2161 tcgatcggtg agcgagttcg cgtcgatcgt gggccatttt ccgtgccagg tgcactgggg
     2221 tgactgcgat cccgccggaa ttattttcta cccaacctat ttccgatgga tggacgccgc
     2281 tacctgggcc ttgatgggct cggtcggcta tgggccgaag cgcatgcgtg aacagcattt
     2341 ggcgatgccc ctggtcgcgg cggactgcca gttcgtcgcg cccgcccagc acggcgatcg
     2401 ctgcgaagtg cgctcgcgaa tcacgcgctt cggcggcgct tcgttcgtgg tcgcgcacga
     2461 catcgtgcgc gacgatggca cgacgctcgc gcagggcagc gaaaagcggg tctggggccg
     2521 ctatgccagc ggacccggca cgcccctgaa aggcgagcac atcccggaag aggtcagggc
     2581 gctgtttcgc gccagatagc gagtacgcgg tctatcatcg gcttcgcgga gccgaaggcg
     2641 cgtttcgcct cgaacagcgc ttccaactcc cccgccggaa tgatccttgt aacgtcgtcc
     2701 tcggcgagca gaacttcacg caagtgcttt ttctccgcga cggcgcgggc gcagagtttt
     2761 tcggtcaacg tgtgcgcggc cgacttgccg agcgtctgcg caagtcggac agacaccgcc
     2821 tcggagaaca ccaggccttg ggtccgctcc aggtttgccg ccatagtatc ggtgcgtacc
     2881 tccaggcgtt cgatcgcttc cgccatggcg gcaacgccgc tcgccgtagc gcacacgagg
     2941 cttcttagcg tcagccattg cgcctgccac tgaccgaggc cgcgctcgtg ctcgggcaca
     3001 aggttcgcca tcaggctggc cgcaagcgca ggagcgcgct gagaagcttc cagcgcgagc
     3061 acgcaggccg ccgggttgcg cttgtgcggc agggcggatg agccgccgcg accgcggctt
     3121 gcgggctcga acgcctcacc aacttcaggc tgcatcagca gcgaaacgtc gcgcgctatt
     3181 ttcccggcgg cacctaccag gatggcggct tcggcgccga agcgggcgaa gcgatcgcgc
     3241 gcgctgtgcc aggggatggg cggtttcctg agcccgagcc gctcagctag tcgcgtgctg
     3301 accagctcgc catcgcttcc aaaagctgcg agcgtcccgg cggggccacc gaactgcaga
     3361 acgcacacct cttcggccgc gtcgcgaaac tgcctatacg agttcgccac cagcgagagc
     3421 cacactgcag ccttccagcc gaacggtact ggcgtcgcgg gctgcagcag cgtgcgcgcc
     3481 acggtcggcg tatcccggtg gcgatcggca agccgcgctg cggcgtcgcc cagccgacgc
     3541 gcggacacaa gcacgcggtg cgccgcttcc ttcagacaga cgaccaccgc ggtatcgatc
     3601 acgtcctggc tggtcgcgcc gaagtggaca tagcgcgcgg cttcaccagg cacctgcgca
     3661 gtcagttcct tgacgaacgg gattgcgagc gtacccgcat gcctcgcttc attcgcgagc
     3721 cttgcgacat caaaagaggc gttctcacaa gcgcgtgcaa tggtttcagc atgctcgagc
     3781 ggaacaagac ccgtgctcgc gcgggcgagc gccgcttcaa agcgcgccat agctgcgagc
     3841 agcttctcgt ccgagagtgc ttcggcggta tgcgggtcgg aaaagacgag ctcgaggctc
     3901 agaaatcgaa gaacaccgtc tcgttcttgc cctgcaggac cacatcccac tcgaactcgc
     3961 cgtcggcgcg cctcttcgcg atcagcgtgc cacggcgctc agcggggatg aggttcagca
     4021 ccgggtcgtc ggcgttggcc ggatcgtcgg gaaagtagat acgggtttga agctgcttga
     4081 gcagcccacg catgaagatg gtgacggtaa tgtgcggcgc ctgcagcttg cggtcggggc
     4141 ctggcacgcg gccgggcttg atggtacgga agcggaaggc gcccccatcg tcggtaagcg
     4201 aacggccgta gccgcgaaag ctctgctcta tcggcttcgc ttgtgcgtcc tcggggcttg
     4261 cgtacttgcc gcggctgttg gcctgccaga tctcgaccgc ggcatcgttg accggcctgc
     4321 cgtcgccgtc gagcacccgt ccctcgatgc gcacgtgctc gcccgcaacg ccttgcggcg
     4381 ccaggtcctc gatcaccatc cattccagcc cgatccgcag atatgggccg atcgtctgtg
     4441 aggtcgtggc gcgcaggctc atggacggtt ctccatcggg gtcgcctcgc gcccgcgcag
     4501 cacgatgtcg aagcgatagc cgagcgcgaa ctcgggctgc gtggtctccc agtcgaaaat
     4561 ggaaatcaga cgattgcgcg ccttctgatc cggcacgcag gtgaacatcg ggtcgtactc
     4621 gagcagcggg tcgcccggaa agtacatctg cgtgatgagg cgctggacga tcgccgggcc
     4681 gaagagcgag aaatgaatgt gcgccggccg ccaggcgttg tagtgattgc gccacggata
     4741 ctcccccgga cgaatggtca tgaagcggta gcgcccttcg gtatcggtca gcacgcggcc
     4801 tgcgccggtg aaattggggt cgagcggcgc gtcatgctgg tcgacgcggt ggttgtagcg
     4861 cccggcggca ttcgcctgcc aaatctcaat gagaatgccg ggaagcggcc ttgagttctc
     4921 atctagcacg cggccgctca cgacgaggcg ctcgcccagc ggctcgcctt cgtgctgctg
     4981 ggtgaggtca ttatccgtcg gcttgatgtc gccgtagccg aatatgggtg cggtgagctc
     5041 cgatagcgta tgcgccagcg ccactagcgg ctgcgtcggc gcgcgtctta tcgtggatgc
     5101 gtacggcgga tagaggtatg gcggctgggt atcccggtac ggtcggcgat agggggctgg
     5161 atcggtcatc gtgtcattcc gcgtcaggtt gcttagcggg ctgcgcgcgc tcgaacgcct
     5221 caagtttcat gcactcttcg aacacgcgca tcgtcgccgg gtaggaagcg aggtcgcagt
     5281 tatagcgctt cgcgttgaag acctgcggca ccaggcagca gtcggccatc gtgggggtgt
     5341 cgccgtggca gcacttgctc tttttgctgc gaagcaggtc ggcttccagc ttggcgaggc
     5401 cgtcggcgat ccagtgacgg taccaggcat tgacctgctc ttcgttatgg ccgagctcgc
     5461 gcctgaggta ttgcagcgtg cgcagattgt taagcggatg gatttcgcag gcaaccagca
     5521 gcgcgaggct gcggactcgc gcacggcctg gcgcatcgcg aggaagaagc ggtgggtttg
     5581 ggtgcgtctc gtcgaggtac tcgatgatgg cgagcgattg cgacagttcg agcccgtccg
     5641 taagcagagt cggcagaagg ccctgcgggt atttgccggc gtattccgac tttcggtgct
     5701 cgcctttggc gagatgaatg aacacgggct cgtaggcgag gcctttcaga ttgagcgcaa
     5761 tgcggacacg aaacgcggcg gagctgcgaa agtacgtgta cagcttcaca acggcggcag
     5821 gattttcacc ttgagctcgc cgacgccgtc gatcccggcg tgcaactcgt cgccgggctt
     5881 gaccgggcca acgccggcag gcgtcccggc atagataaga tcccccggct cgagcgtgta
     5941 gtactcggaa aggtaagcga tctgttccgg caccgaccag atcatgtcgc cgaggtccgc
     6001 gcgctggcgc tgtttgccgt tgactgtgag ccaaatagcg ccgcgccccg ggtggcctat
     6061 cttcgccgcc ggtaccagcg cgccgcatgg cgccgcctgg tcgaagtcct tgccgaaatc
     6121 ccacggcctg ccgtggtcct tgccccattg ctgcaggtcc cggcgcgtca tgtcgaggcc
     6181 gacggcgtag ccaaacacgt gatcgttgcc acgcttggcg tcgatgcgtc gcccgccttt
     6241 cgcaagggcg accaccagct cggtttcgta atggtagttg gaggtcttcg cgggatagcg
     6301 cacctcgccg ccacccggca cgagggcgtg cgcgctcttg atgaagaaaa acggctgctc
     6361 gcgcccgtcg ccgcccatct ccttctggtg ctcggcgtag ttgcgaccga cgcaaaagat
     6421 gcgccggatc ggaaagctat cagccgtgcc agcgaccgga agtaccggcg gggtccacag
     6481 cgggatggcg taacccatga cgctcctcga aaaggcgaaa ttttatcccg cgtagtccgg
     6541 ggcaacgcgg atggcaaatt cgccgatacg ttcgattccg gcgcggatct cgtcgccggg
     6601 cttcactggg cccacgcctt ccggcgtacc gctgaagata aggtcgcccg ggtagagcgt
     6661 atagaagctc gtcgcgtact cgatcagtcg cggaacatcg tagaccatgc ggctcgtgtt
     6721 cgagcgttgc ctcggctcgc cgttgaccgt gagccacagc tcgagatttc ccgggtccgg
     6781 aacctcgtct cgcgtcacga gccacggcgc gagcacgctg tatgtgtcga tcgacttgcg
     6841 aaagcttgga agctcggggc cgcgcaacgt catgtcgagg ccgatcgagt agccgcagac
     6901 gtagtcgagc gcggtcgcgc ggtcgacgcg gtttgcctcg cggccgatga ccacagcgag
     6961 ctccacctcg tggtcgttgc ggcgctcgcg aaagcgcagc ttgatgccgt cgcccgcgcc
     7021 ggcgagcgaa cttggcgact tcagaaagag cccccaatcc gcgatggtct tgatttcccg
     7081 cccgtgcgcg atgccctggt cgcgcttcgc ctcgtcgatg tgtgcctggt agttgatcgg
     7141 agcattgacg atcttcgaag ggttggcgac cggcgggcgc agccggactg cgaaaagggg
     7201 gcggcgtgcc gcgcgccctg ccagtgcctt tgccttctcg agtaccgcgg gcaggtgagt
     7261 gatcagcgcg tcgccatggc ggtagggcca cttcggcggc ggaatagtgg cgagcgcctc
     7321 ggtcacatcg agtacctcgc tgccttcgac caatccgagg cggtcgtcgt cgaagcggca
     7381 aatcttcatc ggctgaacgg tcgacgaatg accgtgtcgt ccctgacctg cgtgccgcag
     7441 gcgagcaggg tgtcgcccga ctgcacctgc acgggatggc cttccaaggt gatttgcatg
     7501 cgccgcgcgg agaacttacg atggtaccaa tcgtttggag cccaaacaaa cgttgcggcc
     7561 cggcctcttg cccgggcttc tcggctaccg tttgcgcctc gcgcagcagg cggtgttccg
     7621 cgacttcgga gagagcgtaa gcgatctgcc gcccggccgc gtcgggatcc tgctaatcat
     7681 cgacgcgaat ccgggggtaa cccagacccg gctagcgcag gcggtcggcc tggaccgctc
     7741 cacgctcgtc ggcgtgcttg acgcactcga agcacgcggc ctggtcgagc ggcgccgcgg
     7801 aaaggaccgg cgtacgaacg gattgcttct cacgcgcgcc ggtcgcgcgc gcgtcactga
     7861 gctcaaacgg cgcatccggg tgcacgagcg gcgggtcgcc gcgcggctca ccgccgagga
     7921 gaagactcag cttctggcat tgctttccaa gctggccgga agatgaaaat ggcgccgaag
     7981 cgccattgtt atcgagagta gccgcttaca ttcgatatgc cgcttccccg tgcgcagcgg
     8041 tatccaggcc ctcgcgctca tcttcctccg tcacacgcag cccgatcgtg aggtcgacca
     8101 gcttgtatgc aatcgcggcg acgatgccgg accataggat agtcaagccg accgccttcg
     8161 cctgcaccca gagttgctcg ctaatggagc tgaagccagt cttggccgtg acccagtcgg
     8221 tgacgaaccc gggtccgccg aggtccgggg agttgaatac gccggtgagc aatgccccga
     8281 gaatcccgcc gaccgcgtgc acgccgaaca catcgagcga atcgtcggca cccagcagct
     8341 tcttcaggcc gcttacgccc cagaggcaga cgagtccagc cgcgaggccg
//
LOCUS       DASTPD010000115         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1284636, whole genome shotgun sequence.
ACCESSION   DASTPD010000115 DASTPD010000000
VERSION     DASTPD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284636"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..761
                     /gene="msrA"
                     /locus_tag="VFR57_04015"
     CDS             102..761
                     /gene="msrA"
                     /locus_tag="VFR57_04015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0258573.1"
                     /translation="MFAFMKKMKSLLPRESTLPGRTEAMRVPAKHFVNGHSLKPLFPE
                     GMQLAQFGMGCFWGAERVFWETPGVYSTAVGYAGGETKNPTYEEVCTGFTNHTEAVLV
                     VFDPKVVTYEELLKVFWESHDPTQGMRQGNDAGTQYRSAIYTYGDAQAQAAARSKEMY
                     EKALTAKGYGPVTTEIRPAPEFYYAEEYHQQYLAKNPGGYCGLGGTGVSCPVGVATAA
                     G"
     gene            772..1128
                     /locus_tag="VFR57_04020"
     CDS             772..1128
                     /locus_tag="VFR57_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU0258574.1"
                     /translation="MARFFKTRSLAQQAIAAGRVKLNGSRVKSSHDLRIGDSVEVRVG
                     DFEWQVTVKALSEKRGPAVEARKLYEETAQSRVERERRMDLRRWGAEPAAALKGRPTK
                     RDRRLIDDFTSPTDES"
     gene            complement(1103..1660)
                     /locus_tag="VFR57_04025"
     CDS             complement(1103..1660)
                     /locus_tag="VFR57_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0258575.1"
                     /translation="MRVTLLLFLFLACPALAEPSPELLSRLREGGYVLYLRHTSTDFS
                     QNDSRMTSFEDCASQRNLTDRGREEARAIGEHVKRLKIPIGEVLASPFCRTMETARLA
                     FGKAMATTEARGGPASDEAGRYAALKKLLSTKIPEGKNLVISSHGNPFYAVAGPPYLA
                     EGEIAVVKPEGERFSVVARIRLSDW"
     gene            1709..>2639
                     /locus_tag="VFR57_04030"
     CDS             1709..>2639
                     /locus_tag="VFR57_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU0258576.1"
                     /translation="MIVAAAAFAQEDAVVITATRVPQPSLEVPASVDRIYADEIREGR
                     PQVNLSESLGRVPGIVVQNRQNYAQDLQISSRGFGARATFGVRGIRLIADGIPATMPD
                     GQGQASTFALGSAERIEVLRGPFSALYGNAAGGVIVVDTEDPPEKPTIGADVMVGDFG
                     TWRAGVLFGGYNALLDASRFETDGYRDHSAARRDHYNAKLKFGLRPDTSLTIIGNQLR
                     QPDTQDPLGLTRAQMEADPRQAAPVAAQFNTKKSIDQGQLGGTYSQRVGEGRVEAMFY
                     GGQRQVEQFLAIPVLTQLAPTHSGGIVDLDRSYG"
BASE COUNT          471 a          852 c          900 g          416 t
ORIGIN      
        1 gccccgaacg cgcgagccac gcggccatca aaccaccgat ggcgccggcg ccgtaaacgc
       61 agattttcat agtgctattc tacgttttcg aggagtgacc catgtttgcc ttcatgaaga
      121 agatgaagtc gctgctgccg cgggaaagca cgcttccggg gcgcaccgag gcgatgcgcg
      181 tgccggcgaa gcacttcgtg aacggccatt cgctcaagcc gctgtttccc gagggcatgc
      241 agctcgcgca gttcggcatg ggctgcttct ggggcgccga gcgggtgttc tgggaaacgc
      301 ccggcgtcta ctccaccgcg gtcggctacg ccggcggcga aacgaagaac ccgacctacg
      361 aagaggtgtg cacgggattc accaaccaca ccgaggcagt gctcgtcgtg ttcgacccga
      421 aggtcgtcac gtacgaagag ctcctcaagg tgttctggga atcacacgat ccgacccagg
      481 gcatgcgcca aggcaacgac gccggcaccc aataccgctc cgccatatat acatacggtg
      541 atgcccaggc ccaggccgct gcgcgctcca aggagatgta cgagaaagcg ctgacggcaa
      601 agggctacgg cccggtcacc accgagatcc gccccgcgcc cgagttctac tacgccgagg
      661 agtatcacca gcagtacctc gccaagaacc ccggcggcta ctgcggcctc ggcggtaccg
      721 gcgtgtcatg ccccgtgggg gtcgccactg cggctggata agtggctctg ggtggcgcgc
      781 ttcttcaaga cgcgcagtct cgcccagcag gcgatcgccg cggggcgcgt gaagctcaat
      841 ggctcccgcg tcaagtcctc tcacgatctg aggatcggag attccgtgga agtgcgcgtg
      901 ggggacttcg agtggcaggt gacggtgaaa gccctctcgg aaaaacgcgg cccggcggtg
      961 gaggcgagga agctctacga ggagacggcg caaagccgcg tggagcgcga gcgacgcatg
     1021 gacctgcgcc gctggggcgc ggagccggcc gccgcgctca agggccggcc gaccaagcgt
     1081 gaccgccgcc tgatcgacga ctttaccagt ccgacagacg aatcctagcg accacgctga
     1141 accgttcgcc ctcgggcttc accaccgcga tctcgccctc ggcaagatag ggcgggcctg
     1201 cgacggcgta gaacgggttg ccgtggctgg aaatgacgag gttcttgccc tcagggattt
     1261 tcgtcgagag cagctttttc aaggcggcat agcgtccggc ctcgtcgctt gccgggccgc
     1321 cgcgcgcttc cgtggtcgcc atagctttac cgaaggcaag tcgcgccgtc tccatggtcc
     1381 ggcaaaacgg gctcgcgagg acttcgccga tcggaatctt caggcgtttc acatgttcgc
     1441 cgatggcgcg cgcctcctca cgcccgcggt cggtgaggtt gcgctggctc gcgcagtcct
     1501 caaagctcgt catgcgcgag tcgttctggc tgaagtcggt cgaggtgtgc cttaggtaaa
     1561 ggacgtagcc gccctcgcgc aggcggctca ggagctcggg gctcggttcg gcgagcgccg
     1621 gacaagcgag aaagagaaac aataggagcg tgacgcgcat tcaaggtagc ctagcaggca
     1681 aatgaagaag atggctggag gggggctgtt gatcgtcgca gcggccgcgt ttgcgcagga
     1741 ggacgcggtc gtcattacag caacgcgcgt gccgcagccg agcctcgagg ttcccgcatc
     1801 ggttgaccgg atctatgcgg acgagatccg ggagggtcgg ccgcaggtga atctgtccga
     1861 gagcctcggc cgcgtgccgg gcatcgtggt acagaaccgg cagaactacg cgcaggacct
     1921 gcagatttcc tcccgtgggt ttggcgcgcg cgcgactttc ggcgtgcgcg gcatccgcct
     1981 catcgccgac ggcattccgg ccaccatgcc cgacggccag gggcaggcct cgaccttcgc
     2041 gctcggctcg gccgagcgca tcgaggtgct gcggggcccg ttctccgcgc tctacggcaa
     2101 tgcggccggc ggggtgatcg tggtcgatac cgaggatccg ccggaaaagc ccaccatcgg
     2161 cgcagacgtg atggttgggg acttcggcac ctggcgcgcg ggcgtgctgt tcggtggcta
     2221 taacgcgctc ctcgatgcat cgcgcttcga gaccgacggc taccgggatc acagcgcggc
     2281 gcggcgcgat cactacaacg cgaagctgaa gttcgggctg cggccggaca cctcgctgac
     2341 gattatcggc aaccagctgc gccagcccga cacgcaggat ccgctcggcc tgacgcgcgc
     2401 gcagatggag gccgatccgc ggcaggccgc gccggtggcg gcgcagttca acaccaagaa
     2461 aagcatcgac cagggccagc tcggcggcac ctacagccag cgcgtgggcg agggccgcgt
     2521 cgaggccatg ttctacggcg ggcagcgcca ggtggagcag ttcctcgcca tcccggtgct
     2581 gacgcagctc gcgccgacgc attccggcgg catcgtcgac ctggaccgca gctacggcg
//
LOCUS       DASTPD010000116         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1080867, whole genome shotgun sequence.
ACCESSION   DASTPD010000116 DASTPD010000000
VERSION     DASTPD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080867"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VFR57_04035"
     CDS             <1..148
                     /locus_tag="VFR57_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0258577.1"
                     /translation="AFFIIAIIAAVFGFTGIAAGAAEIAKILFVVFVVLFLVSLVAGL
                     LRRP"
     gene            177..692
                     /locus_tag="VFR57_04040"
     CDS             177..692
                     /locus_tag="VFR57_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258578.1"
                     /translation="MKVKLTAVTLGLAFGLASAAQAQTTPAPGKRDSAPPRAEQSERK
                     AKDAAEERIEGEYKAAREKCNSMEGNAKDICMAEAKGKQKVAKAELDAKDAKDQAKAQ
                     RKVQEARADAAYEVAKEKCDDQKGEAKDACVKQARVERDRAKGKAERTAEARQEKQRA
                     ATGSTAPEKQK"
     gene            759..1580
                     /locus_tag="VFR57_04045"
     CDS             759..1580
                     /locus_tag="VFR57_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0258579.1"
                     /translation="MARGLWKGAISFGLVHVPVELHSAKKRSTELDMTMLDKRDLAPV
                     GYKRVNKSTGKEVPWADVVKGYEYEDDKYVVLSDEDFRRANPEAAKTVDIQAFVELID
                     IAPQYFDTPYYLVPGKRGEKAYALLRDTLKKAGKAGIASVVIRTRQYLAALMAQDDIL
                     VLNTLRHHDELKDPSELKVPEARVSTKELDMAMRLVEDMADEWKPERYRDTFRDDLLK
                     RIDEKVKAGRTEEITEPEKERKAEKGAEVIDLMELLKNSIEKKPAKRAARKRRAA"
     gene            1577..1804
                     /locus_tag="VFR57_04050"
     CDS             1577..1804
                     /locus_tag="VFR57_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258580.1"
                     /translation="MKGRGSDMATCEVCGNSYAKAFSVKMPDGAAHTFDSFECAIAKL
                     APTCAHCNCRVIGHGVELDGKIFCCEHCAHA"
     gene            1797..2888
                     /locus_tag="VFR57_04055"
     CDS             1797..2888
                     /locus_tag="VFR57_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0258581.1"
                     /translation="MPKRQIRYAVVGLGHIAQVAVLPAFANARRNSRLAALVSTDAVK
                     LQQLSQKYGLQRTFTYEEYETCLKSVDAVYIALPNHLHCDYAVRAARAGVHVLVEKPM
                     AVTEEECERMIHAAREAGVKLMVAYRLHFERANLEAGEIARSGRIGEPRLFNSTFCTP
                     VVPGNIRARRETGGGVLYDIGIYCINAARSLFRDEPVEVRALTSGRLDEVEESAAAVL
                     RFPNDRLAAFTASFGTEKVSEYRLAGTKGDLAVEPAYEYARPLKHRLTLEGETRERRF
                     AKRDQFAPELLYFSDCILQNGTPEPGGDEGLADVRVIRALYRSAENGQPVALAPFAKR
                     ARPSLEQEIRRPPVEKPDVIHAAAPSGAR"
     gene            2915..3274
                     /locus_tag="VFR57_04060"
     CDS             2915..3274
                     /locus_tag="VFR57_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0258582.1"
                     /translation="MKGIIAIVVAAAFTLPACAQKSGSSLPDTQDRAQNRETVSSSTS
                     NATLTGKVKTALASDVGLGTVTSINVDSDNGVVTLKGHVDSADKKAQAEEIAKKVDGV
                     KSVKNELRVEPAKDKSS"
     gene            3437..3949
                     /locus_tag="VFR57_04065"
     CDS             3437..3949
                     /locus_tag="VFR57_04065"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEU0258583.1"
                     /translation="MRTTTLALIFGIAYLAAGVLGLIPAALLPPPADAPPTAFAVLYG
                     YLIGLFPVNILHTAVHLAIGVWGIAAWRSAGGARVYARSLAILYGVLAVMGLIPGLNT
                     VFGLIPLHSHDVWLHTGTAVIAAYFGWRNEEVHMERRMSGERRQRMIPVARERRLSVA
                     DRRELGYGGA"
     gene            4122..4334
                     /locus_tag="VFR57_04070"
     CDS             4122..4334
                     /locus_tag="VFR57_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258584.1"
                     /translation="MLPVHDVDCPKCGQRVYTAISLEDVIPADAPTVPKVSSDARGYH
                     LRCPHCRARIDMKRVDTPGGTGFRLA"
BASE COUNT          870 a         1436 c         1515 g          653 t
ORIGIN      
        1 ggcgtttttc atcatcgcaa tcatcgcggc ggttttcggc ttcaccggca tcgctgccgg
       61 agcggccgag atcgccaaga tcctgttcgt cgtgttcgtg gtccttttcc tcgtctcgct
      121 ggtggcggga ctgctgagaa ggccttagag acaaagctaa aggagagata cgtgctatga
      181 aagtgaaact caccgctgtc acgctgggcc ttgccttcgg cctggcgagc gccgcgcagg
      241 cgcaaaccac cccggcgccg ggcaagcgag attccgcgcc gccgcgcgcc gagcagtccg
      301 agcgcaaggc gaaagacgcc gcggaagagc gcattgaggg ggagtacaag gccgcgcgcg
      361 agaagtgcaa cagcatggaa ggcaacgcca aggacatctg catggccgaa gcgaagggca
      421 agcagaaggt cgccaaggcg gagctcgacg cgaaagacgc gaaggaccaa gccaaagcgc
      481 agcgcaaggt gcaggaagcc agggcggacg ccgcctacga ggtcgcgaag gagaagtgcg
      541 acgaccagaa gggcgaagcc aaggacgcgt gcgtcaagca ggcgcgcgtg gagcgcgacc
      601 gcgccaaggg caaggcggag aggactgccg aagcacggca ggagaagcag cgcgccgcaa
      661 ccggtagtac tgcgcccgag aagcagaagt agcgatccgg agcgttcctc ctcgcaagag
      721 gaggacacgc cacggaggga tcacgccggc agcagccgat ggcgcgcggg ttgtggaaag
      781 gagcaatcag cttcgggctg gtccacgtgc cggtcgagct gcattcggca aagaagcgct
      841 cgaccgagct cgacatgacc atgctcgaca agcgcgatct cgccccggtc ggctacaagc
      901 gcgtcaataa gtcgacgggc aaggaagtgc cgtgggcgga tgtggtaaaa ggctacgagt
      961 acgaggacga caagtacgtc gtgctctccg acgaggattt tcgccgcgcc aatccggagg
     1021 cggcgaagac cgtcgacatc caggcgttcg tcgagctgat cgacatcgcg ccgcagtatt
     1081 tcgatacacc ttactacctc gtcccgggca agcgcggcga gaaggcttat gcgctgctcc
     1141 gcgacacgct caagaaggcc ggcaaggccg ggatcgcgag cgtggtgatc cgcaccaggc
     1201 agtacctggc ggcgctgatg gcgcaggacg acatcctggt gctgaacacg cttcgccacc
     1261 acgacgagct gaaggacccc tccgagctga aggtgccgga ggccagggtt tcgacgaagg
     1321 agctcgacat ggcgatgcga ctggtcgagg acatggccga cgagtggaag cccgagcgct
     1381 accgtgacac cttccgcgac gacctcttga agcgcattga cgagaaagtg aaggccggcc
     1441 ggaccgagga gatcaccgaa cccgagaagg agcgcaaggc cgagaaggga gccgaggtca
     1501 tcgacctgat ggagctcttg aagaacagca ttgagaagaa gcccgcgaag cgcgccgcaa
     1561 gaaagcggcg cgccgcatga aaggaagagg aagcgacatg gcgacttgcg aagtctgcgg
     1621 caacagctac gcgaaggcgt tctcggtgaa gatgcccgac ggcgccgcac acaccttcga
     1681 cagcttcgag tgcgcgatcg cgaagctcgc gccgacttgc gcgcactgca actgccgcgt
     1741 catcggccat ggcgtcgagc tcgacgggaa gatcttctgc tgcgagcact gcgcgcatgc
     1801 ctaagcggca gatacgctac gccgtggtcg ggctcgggca catcgcccag gtggcggtgc
     1861 tgcccgcgtt cgccaacgcg cggcgcaact cgcggctcgc agcgctggtg tcgaccgacg
     1921 ctgtcaagct gcagcaactg tctcaaaagt acggcctcca gcgcacgttc acctatgaag
     1981 agtacgagac ctgcctgaag agcgtggatg cggtgtacat cgccctgccc aaccatttgc
     2041 actgtgacta cgcggtgcgg gcagcgcgcg ccggcgtgca cgtgctcgtt gaaaagccca
     2101 tggcggtaac cgaggaggag tgcgagcgca tgatccacgc ggcgcgcgag gccggggtca
     2161 agctgatggt ggcctaccgg ctgcacttcg agcgcgccaa cctggaggcc ggcgagatcg
     2221 cgcgctcggg ccgaatcggc gagccgcgcc tgttcaactc cacgttctgc accccggtcg
     2281 taccgggcaa catccgcgcg cgccgcgaaa ccgggggcgg cgtgctgtac gacatcggaa
     2341 tctactgcat caacgcggcg cgctcgctct tccgggacga gccggtcgag gtgcgcgcgc
     2401 tgacctcggg caggctcgac gaggtggagg agtcggcggc cgcggttctg cgcttcccga
     2461 atgatcgcct cgccgccttc actgcatcgt tcggcaccga gaaggtctcc gagtaccgcc
     2521 tcgccggcac caagggcgac ctcgcggtcg agccggcgta cgagtacgcc aggccgctca
     2581 agcaccggct gacgctcgag ggcgagacgc gcgagcggcg tttcgccaag cgcgatcagt
     2641 ttgcgcccga gctgctctat ttctcggatt gcattctgca gaacggcacc cccgagcccg
     2701 gaggcgacga gggcctcgcc gatgtacgcg tcatccgcgc gctttaccgt tccgccgaaa
     2761 acggtcagcc ggttgcgctc gcgcccttcg ccaagcgcgc gcggccgagc ctcgagcagg
     2821 aaatccgccg tccgccggtc gagaagcccg acgtgatcca cgccgcggcc ccgtcgggag
     2881 cccgctgatt tttttcaacc gaaggagaca caccatgaaa ggcattatcg ccatcgtagt
     2941 cgcagccgct tttaccctcc cggcatgcgc gcagaagagt ggcagctcgc tgcccgacac
     3001 gcaggatcgc gcccagaacc gcgagaccgt gagcagcagc acgtccaacg ctacgctgac
     3061 cggcaaggtg aagaccgcgc tcgcctcgga cgtcggactt ggcaccgtca ctagcatcaa
     3121 cgtcgacagc gacaacggcg tagtcacgct caagggccat gtcgattcgg ccgacaagaa
     3181 agcgcaggcc gaagagatcg cgaagaaggt ggacggcgtg aagtcggtga aaaacgagct
     3241 tcgcgtcgag ccggccaaag acaagagctc ctgaccgtgg acgactccgg aacgccgttg
     3301 ctttcgatca cgcagacgcg cgacggcgtc tggagcgtca acgagcgggg gcccgcgcgc
     3361 gtcggtgttg ccgcgtggcg ctggtcgacc gcgcacacag aacgctgctc tcgcatgatt
     3421 actcgcagga gaaaacatga ggaccacaac tcttgctttg atcttcggca tcgcctatct
     3481 cgccgccggc gtgctgggcc tgatcccggc cgcgcttctg ccgccgccgg cggacgcgcc
     3541 gccgacggcc tttgccgtgc tttacggcta cctcattggc ctcttcccgg tgaacatcct
     3601 gcatacggcg gtgcacctgg ctatcggcgt atggggcatt gccgcctggc gcagtgccgg
     3661 cggcgcgcgg gtctacgcgc gttcactcgc gatcctctac ggcgtgctgg ccgtgatggg
     3721 ccttatcccc gggctgaaca ccgtattcgg cctcatcccg ctgcacagcc acgatgtgtg
     3781 gctgcatacg ggcaccgcgg tgatcgcggc gtacttcggc tggcgaaacg aggaggtgca
     3841 catggagcgc aggatgagcg gcgaacgtcg gcagcgcatg atcccggtcg cgcgtgaacg
     3901 ccggctgagc gtcgccgatc ggcgcgagct cgggtacggc ggcgcttgag gacgaagtcg
     3961 agggcgctga agagctgggc ctcggggccc gggcgccggg agatcatcaa gcgggccgct
     4021 tctgatgtgg agcgcggtct gaaggacacc gactgtcgcg gcgttcataa gcccgcgcaa
     4081 gcggattgcc cgctccccgc ccggaagcgc cgccgaaaat agtgctgccg gtccacgacg
     4141 tcgactgccc gaagtgtggg cagcgcgtct acaccgcgat ctcgctcgag gacgtgatcc
     4201 cggcggatgc gccgacggtg ccgaaggttt cgagcgacgc gcgcggctac cacctgcgtt
     4261 gcccccactg ccgcgcacgt atcgatatga agcgggtcga caccccgggc ggcaccggct
     4321 tccgcctcgc gtaaatcgca acctttgggc cgcttcccac tgtacgctgc aaggcatggc
     4381 aaccgcgccg ttccgcgccg accacgtcgg cagcctgctg cggccccccg agctcaagga
     4441 agcgcgcgag gccttcaagg ccgggcgcat tgag
//
LOCUS       DASTPD010000117         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1081403, whole genome shotgun sequence.
ACCESSION   DASTPD010000117 DASTPD010000000
VERSION     DASTPD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081403"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFR57_04075"
     CDS             <1..220
                     /locus_tag="VFR57_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEU0258585.1"
                     /translation="VGRLMPAADSLAGGALPLGLAHGWKVLRPVPAGQPVKWSDVAFD
                     ANQTAVRVRREMEQAMSARAASVQEKVA"
     gene            complement(217..1194)
                     /locus_tag="VFR57_04080"
     CDS             complement(217..1194)
                     /locus_tag="VFR57_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258586.1"
                     /translation="MLLAAGLALASRALPSAAQPSPKHLVGFLSGGVTSDVSGFLSSF
                     REAMRELGYREGQNLVLEARYSEYSAERAQKLAAELAALQPRVIVANGGGIGPACRLT
                     PPLPVVFLISGDPVDAGFADSYARPGRNATGVTLLALDLIVKRLELLKQINPKMRKVV
                     FLASPEHAGQKLELAASRAAAEQLGLDVSYHEARTPAELAAALPQVLAVKPDGVLLFS
                     DALMVGQRHSLANFFLKHRIPSAAGWVAFAESGHLVTYGPERHAVWRRLAYFVDRIIK
                     GTRPGALPVELPSVMELAVNRRTATAMGLTLPQGVLFRADRVIDTAPPV"
     gene            complement(1270..2748)
                     /locus_tag="VFR57_04085"
     CDS             complement(1270..2748)
                     /locus_tag="VFR57_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0258587.1"
                     /translation="MEAFLGGMALLLDPKILLVVFAGTLIGIVVGALPGISGSTTTAL
                     LLPFTITMGPVPAIAFLGAIYCAANFGGSITAILVNSPGDPSASATAFDGYPMARRGE
                     AGRALGMSAVASAIGGIFSVIVLIIAAPLLARVAYSFGPPEYFALAVFGLSMVAMTGG
                     DSMLKNLIAGAFGVLLATVGQDLTTGVERYTFGIHSFTDGIEFIPVLIGLFALTELLE
                     QASQLRNRAWIAKLGAVRLPSWDDIRKCSKAIWLSSALGTFIGILPALGATTAAMIAY
                     VETRRWSKNKAEFGKGAIEGVAGPEAANNAAVGGAMVPTLALGIPGSTTTAIILAGLI
                     VQGVRPGPHLFTEQPLLLYAVFSSMLLANLLYLVLGLGFAKVFARISLIPPQFLWPAV
                     FVLSVIGAYAPNQAMAEVWVAIAFGFIGFFMRRYGFSPAPLVIGLVLGRLVEETLKQS
                     LLIFDLSWGGFLGRPIAMTLFGLTALFLLAPLLLPKLRGRPS"
     gene            complement(2749..3183)
                     /locus_tag="VFR57_04090"
     CDS             complement(2749..3183)
                     /locus_tag="VFR57_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0258588.1"
                     /translation="MNSNRVAAAAVAAIAVALFGATFWFDKMPEGITRGFGAEAFPRL
                     VLGTIVALALLLALLSRTGESLAPIPAIVYYSGAALLLFMAIVPLVGMLAAMFLFLLG
                     LGYLWGERRHWVLLVSAALLMVAIWTVFVRGFGVQLPQGIFA"
     gene            complement(3180..4148)
                     /locus_tag="VFR57_04095"
     CDS             complement(3180..4148)
                     /locus_tag="VFR57_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258589.1"
                     /translation="MTDTNRRRLLGFAGGAAALSAFPLRAQDKFPSKPIEVVTHSGVG
                     GGTDITARMMMVHAPGVFKTEFVVVNRTGGSGAAAMDYLLERKDGHTIALITQTHLLT
                     ILRSKGKYKYEDLVPLARATEDPQVLMVGKNSPFKTPQDFVAAGKGKALKWGTTHVGS
                     VDHIGSVAVARAAKHQNPTIVPFRGGGDIVINLVGGNIDCALVNYAEAESQIKAGDIR
                     PLAIFAGSRLKALPNVPTGKEIGVDAGSQSTVRGFVTTRGVPADRVKVLEEGLVKAMQ
                     GQMFTTYLESSGQALDSVTGAGPWKAQLDAFMVEGKSTLENLGLLK"
     gene            4190..>4639
                     /locus_tag="VFR57_04100"
     CDS             4190..>4639
                     /locus_tag="VFR57_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0258590.1"
                     /translation="MQASTALSHVKVLDLTRVRAGPTAVRQLADWGAQVIKIEMPAGL
                     DEGEGPGGPREGSDFQNVHRNKRGMTLNLKSPDGVAILKKLAQNADVLVENYRPDVKD
                     RLGIGYQAMAEVNPRLIYASISGYGEDGPYRMRPGFDQIAQGMGGLMS"
BASE COUNT          853 a         1484 c         1569 g          733 t
ORIGIN      
        1 cgtgggcagg ctcatgcccg cggcggattc acttgcgggc ggggcattgc cactgggttt
       61 ggcgcacggc tggaaggtgc tgcgccccgt tcctgcgggc cagccggtga agtggagcga
      121 cgtcgccttc gacgcgaacc agaccgcggt gcgcgtgcgg cgcgagatgg agcaggcgat
      181 gagcgctcgc gcagcttcgg tacaagaaaa agtcgcctag accggcggcg cagtatcgat
      241 cacgcgatcg gcgcgaaaca gcacgccttg cggcagtgta agacccatgg ccgtggccgt
      301 gcgccgattc acggcgagct ccatgacgct gggcagctcg accggcaggg cgccggggcg
      361 cgtgcccttg ataatccggt cgacaaaata ggccaaccgc cgccacacgg catgccgttc
      421 cggcccgtac gttacaaggt gaccgctctc cgcgaaggcc acccaaccgg cggcgcttgg
      481 aatgcggtgt ttcagaaaga agttggcgag cgagtggcgc tgtccgacca tgagggcgtc
      541 ggaaaagagc aggactccgt ccggcttcac ggcgagaacc tgcggcaagg ctgccgcgag
      601 ctcggcgggg gtgcgcgcct cgtggtagct cacgtccaga ccgagctgct ccgcggccgc
      661 ccgtgacgcg gcgagctcca gcttctgccc agcgtgctcg gggctcgcca ggaacacgac
      721 cttgcgcatc ttcggattga tctgcttcaa gagctcgagg cgcttcacga tcagatcgag
      781 cgcgaggagc gtcacgcccg tagcgtttcg gccgggccgc gcgtagctgt cagcaaatcc
      841 cgcgtccacc gggtcgccgc tgatcaggaa caccactggc agcggtggcg tcaggcggca
      901 tgcgggtccg atgccgccgc cgttggcgac gatgaccctg ggctgcagcg cggcgagctc
      961 ggcggcaagc ttttgcgccc gttcagccga gtattcggaa tagcgcgctt cgagcaccag
     1021 gttctggccc tcgcggtagc cgagctcgcg catcgcttca cggaacgagc tgagaaatcc
     1081 gctgacgtct gaggtcacac cgcccgacag gaaaccgacg aggtgcttcg gcgacggctg
     1141 cgccgcgctc ggcaaagcgc gcgaagcaag tgcaagaccg gctgcaagca gcatccggcg
     1201 tttctggctc gaactcatgc gttctccctg gatcggcgta gctgcgctgc aaagatatag
     1261 cgaaacggct cagctggggc ggccgcgaag ctttggcagc agcagggggg cgaggaggaa
     1321 cagcgcggta agcccgaaga gagtcatggc gatcggccgg ccaaggaagc caccccagga
     1381 aaggtcgaag attagtagcg actgtttgag cgtctcctcg accagtcggc cgagcaccag
     1441 gccgatcacg agcggcgccg gcgagaagcc gtagcgccgc atgaagaagc cgatgaagcc
     1501 gaaggcaatc gccacccaaa cctcggccat cgcctggttc ggcgcgtagg cgccgatcac
     1561 cgagagcacg aagactgccg gccataggaa ctgcggcggg atcaggctga tgcgcgcgaa
     1621 caccttggcg aaaccgagtc cgagcaccag atagagcagg ttggcgagca gcatcgacga
     1681 gaagaccgca tagaggagga gcggctgctc ggtgaaaagg tgcgggccgg gacgcacgcc
     1741 ctgcacgatg aggccggcga gaatgatggc ggtggttgtg ctgccgggga tgccgagggc
     1801 gagcgttggc accatggcgc caccgactgc ggcgttgttc gcggcttcgg gtcccgccac
     1861 gccttcgatc gcgcccttgc caaactccgc cttgtttttc gaccagcgcc gcgtttccac
     1921 gtaagcgatc atcgccgcgg tggtcgcgcc gagcgcgggc aggatgccaa tgaaggtgcc
     1981 gagcgcgctc gagagccaga tcgctttgga gcatttgcgg atgtcgtccc agctcggaag
     2041 ccgcacggcg ccgagctttg cgatccaggc cctgttcctg agctgcgatg cctgctcgag
     2101 gagctcggtc aaggcgaaga ggccgatgag cacggggatg aactcgatgc cgtccgtgaa
     2161 cgagtggatg ccgaaggtgt agcgctccac tcccgtggtg agatcctggc caacggtggc
     2221 cagcagcacg ccgaatgcgc cggcgatgag attcttcagc atgctgtcgc cgccggtcat
     2281 ggccaccatg gaaagtccga ataccgcgag agcaaagtat tccggcgggc cgaacgaata
     2341 ggcaacgcgc gcgaggagcg gcgccgcgat gatgagcacg atcacgctga agatgccgcc
     2401 gatcgcgctc gccaccgccg acatgccgag cgcgcgtccc gcttcgccgc gccgcgccat
     2461 tggatagccg tcgaaagcgg tcgccgacgc cgaagggtcg cccggcgagt tcacgaggat
     2521 cgccgtgatc gagccgccga agttcgcagc gcagtagatg gcgccgagga acgcgatcgc
     2581 cggcaccggg cccatcgtga tggtgaaggg gagcagcagc gcggtggtgg tcgaaccgct
     2641 gatccccggc agcgcgccga cgacgatgcc gatcagcgtg ccggcgaaga ccaccagcag
     2701 gatcttcgga tctagtaaca acgccatccc gcccaggaag gcttccatct aggcgaagat
     2761 tccttgtggc agctggacgc cgaagcccct cacgaatact gtccagatcg cgaccatgag
     2821 cagcgccgcg ctgacgagca gtacccagtg acgccgctcg ccccataagt agccgaggcc
     2881 gagcaggaac aagaacatcg cggccagcat gccgacgagc ggcacgatcg ccataaaaag
     2941 aagcagcgcc gcgcccgagt agtagacgat cgccgggatg ggcgccagcg actcgcccgt
     3001 gcgtgaaagc agggcgagga gtagcgcgag ggcgacgatc gttcccaata cgaggcgcgg
     3061 aaaggcttcc gcgccgaagc cgcgcgtgat gccctcgggc atcttgtcga accagaaggt
     3121 cgcgccgaac agcgccaccg cgatggcggc caccgccgcg gccgccacgc ggttggagtt
     3181 cacttcagca gaccgaggtt ctccagcgtg ctcttgcctt caaccataaa tgcgtcgagc
     3241 tgcgccttcc acggacccgc gccggtcacg ctgtcgagcg cctggccgga actctcgagg
     3301 tacgtggtga acatctgtcc ctgcatcgcc ttgaccagac cctcttcgag caccttcacc
     3361 cggtcggcgg gcacgccgcg cgtggtcacg aagccgcgca ccgtggactg gctgccggcg
     3421 tcgacgccga tttccttgcc ggtcggtacg ttgggcagcg ccttcagccg gctgccggca
     3481 aagatcgcga gcggacggat gtcgcccgcc ttgatctgcg actcggcctc ggcgtaattc
     3541 actagcgcgc aatcgatatt gccgccgacc aggttgatca cgatgtcgcc accgccgcgg
     3601 aatggcacga tcgtcgggtt ctggtgcttg gcggcgcgcg ccaccgcgac cgagccgatg
     3661 tgatcgacgc tgccgacatg cgtcgtgccc cacttgagcg ccttcccttt gcccgcggcg
     3721 acgaagtcct gcggcgtctt gaacgggctg ttcttgccga ccatcagcac ctgcggatcc
     3781 tcggtcgcgc gcgcgagtgg cacgaggtct tcgtacttgt acttgccttt gctgcgcagg
     3841 atggtgagca gatgcgtctg tgtaatgagc gcgatggtgt ggccgtcctt gcgctcgagc
     3901 agatagtcca tcgccgcggc gccgctgccg ccggtgcgat tcaccacgac gaattccgtc
     3961 ttgaatacgc cgggcgcgtg gaccatcatc atgcgcgccg tgatgtcggt gccgccgcct
     4021 acgccggaat gcgtgacgac ttcgatcggc ttcgatggaa acttgtcctg cgcgcgcagc
     4081 gggaatgcag agagcgctgc cgctccgcct gcgaaaccga gcaggcgcct gcgattcgta
     4141 tcggtcacgt tcggttttcc tcctggggca gcagtgtaga ctcgaaacga tgcaagcctc
     4201 caccgctctt tcgcatgtca aggttctcga tctcactcgc gtgcgcgcgg gtcccacggc
     4261 cgtgcgccag ctcgccgact ggggcgcgca agtgatcaag atcgaaatgc cggccgggct
     4321 cgatgaaggc gaagggcccg gtggcccgcg cgaaggttcg gactttcaga atgtgcatcg
     4381 caacaagcgc ggcatgacgc tgaatctcaa gtcgcccgac ggcgtcgcca tcctgaagaa
     4441 gctcgcgcag aacgcggacg ttctcgtcga gaactatcgt ccggacgtga aggatcggct
     4501 gggtatcggc taccaggcta tggccgaggt gaatccgcgt ctcatttacg cgagcatctc
     4561 gggctacggc gaagacggcc cgtatcgcat gcggcccggc ttcgatcaga tcgcacaggg
     4621 catgggcggc ctcatgtcg
//
LOCUS       DASTPD010000118        10628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_60725, whole genome shotgun sequence.
ACCESSION   DASTPD010000118 DASTPD010000000
VERSION     DASTPD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60725"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFR57_04105"
     CDS             <1..402
                     /locus_tag="VFR57_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0258591.1"
                     /translation="EIYREPASPFVADFVGKVNVLAGTAENGGLRIGAAVLECNGAHV
                     PKSHSVKIYLRPEDIVARPIAPGDSNVFDARIEKVEFLGSYCLVRVATDVLGQPLTVY
                     LSLNFLSEQALAPGSRLKLRILPDRLRLFDA"
     gene            395..2095
                     /locus_tag="VFR57_04110"
     CDS             395..2095
                     /locus_tag="VFR57_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEU0258592.1"
                     /translation="MPEIAVAIRQRTDPADRIGHAGLLVVFVLLMAFLVAPLAAVLMQ
                     SVEDRNGHFVALDNFVAYVQTPALVHSLWNSIWVSAVVTLLTIPAAFLFAYALTRSCM
                     PLKGVFRTIALTPLLAPSLLAAISFIYWFGNQGVLKDAMQALGIASIYGAVGIIVSEM
                     FAVFPHALMILITALTLADARLYEAADALGTSAARKFLTITLPGAKYGLISAALVTFT
                     LVITDFGIPKVIGGNFDVLATDIFKLVIGQQDFQRGAVVAVLLLASALITFFVDRLIQ
                     RKQTSMLTARAVPYSPRPSPGFDLAMTLYCALIAALMLAVFGMALYASFVKFWPYDLS
                     FSLRHYAFGLVDAEVDTAFFNSLKLALGTAIGGTVLVFVGAYLLEKTRGVEWLRPGMQ
                     LLALVPMAVPGLVLGLGYIFFFNSPGNPLNGLYHTMTLLVLCTIVHFYTTGHLTAVTA
                     LKALDAEFEAVSASLKVPFYRTFLRVTLPICTPTLLDISRYFFINAMTTISAVVFLYS
                     PDTKVASIAILNLDEAGEPGPAAAMAVLIAATSLAACVVYLGLGWLVERKTQKWRNLK
                     "
     gene            2077..3246
                     /locus_tag="VFR57_04115"
     CDS             2077..3246
                     /locus_tag="VFR57_04115"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEU0258593.1"
                     /translation="MEKPQVKEPILLTPGPLTTSTETKLAMVRDWGSRDTGFIELNRR
                     VRQRLLDVVGVRETHVCVPLQGSGTFAVEAMLGTMVPKSGHVLVPLNGAYCRRIQRIC
                     SILGRKVTPIEYDEREPVEPADIDAALKADPTISHVALVHCETSTGVLNPLHEISEIV
                     ARHRKSLLVDAMSSFAAVPIEGPFDALVAASGKCLEGPPGMGFALMRKSALEKCTGNS
                     PSLVLDLHDQWVNMEKTAQWRFTPPTVIVAALHAALEQFVAEGGRAARGARYGRNCEV
                     LIEGMTKLGFKLFLDRKHQAPVIVTFHAPSDPNYDFQRFYDRVREKGFVLYPGKLTQV
                     DTLRVGCIGAIDEHAIRAAVHAIADTVTEQSIRNCGVSSSTPPAERAARGGPWPT"
     gene            complement(3197..3979)
                     /locus_tag="VFR57_04120"
     CDS             complement(3197..3979)
                     /locus_tag="VFR57_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEU0258594.1"
                     /translation="MAEAPDYEPEVAQPGPIGLRRRGIYLLPNLFTTGVLFCGFFAIV
                     QAMNGRFEIGAIAIFVAMVLDGMDGRVARWTNTQSEFGAQYDSIADMVAFGAAPALMT
                     YVWVLKELGTAGWIGAFIYCAGAGIRLARFNANIAVVDKRYFQGLPSPSGAAIMAGLV
                     WVLVDHDFVPREHVWIAAVTWAVAVFVGVTMVSNVPFWSFKEVNWKKRVPLWAILALV
                     VGFSIVSLSPSLVLFGFFLAYGLSGYVMWAMGHRVRPVLPEE"
     gene            complement(3946..4617)
                     /locus_tag="VFR57_04125"
     CDS             complement(3946..4617)
                     /locus_tag="VFR57_04125"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU0258595.1"
                     /translation="MKQRRYPHPLIAREGWLFVGIAVAVSLLVGFWLGWWWSVPLWLG
                     AIFILQFFRDPPREVPDAPGAVLSAADGRVVEVSRAQDPYVKRDALKVSVFMNVFNVH
                     SNRSPVDGVVKERWYFPGAFVNAALDKASLENERNALWLHTPEGHDVTCVQVAGLIAR
                     RILCYVGTGAELKRGQRFGFIRFGSRVDHYLPLDVEVKAAIGDKVYAAESVLAWLKPP
                     TTSPK"
     gene            complement(4614..5630)
                     /gene="ilvC"
                     /locus_tag="VFR57_04130"
     CDS             complement(4614..5630)
                     /gene="ilvC"
                     /locus_tag="VFR57_04130"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0258596.1"
                     /translation="MKVYYDKDADLSLVKGKKVTIIGYGSQGHAHALNLQESGVKVTV
                     GLRKGGASWDKAKKAGVKVAELADAIKGADIVMILLPDEHHAAVYQQFVEPNIKKGAS
                     LAFAHGFNIHYGLIQPRADLDVWMVAPKAPGHTVRSTYAAGGGVPMLIAVHRNPSKKA
                     RDVALSYAAAIGGGKAGIIETTFREETETDLFGEQTVLCGGCVELVKAGFDTLVDAGY
                     APEMAYFECLHELKLIVDLMYEGGIGTMNYSISNNAEYGEYVTGPKIIDEGVRKRMKE
                     ALHRIQNGEYAKEFVLENRAGAPGLLSQRRMTAELPIEKVGARLREMMPWIKRNRLVD
                     TSKN"
     gene            complement(5647..6138)
                     /gene="ilvN"
                     /locus_tag="VFR57_04135"
     CDS             complement(5647..6138)
                     /gene="ilvN"
                     /locus_tag="VFR57_04135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU0258597.1"
                     /translation="MRHIISILVENEAGALSRIAGLFSARGYNIETLTVAPTEDASMS
                     RMTIVTTGSEDVVEQITKQLNKLIEVVKVIDLSEAEHIERELMLIKVRAGQKEREDMK
                     RMADIFRGRIIDVSDNTYTIELTGDGAKLDAFIRSLDQGAIIETVRTGASGIGRGNRV
                     LRV"
     gene            complement(6138..7850)
                     /locus_tag="VFR57_04140"
     CDS             complement(6138..7850)
                     /locus_tag="VFR57_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEU0258598.1"
                     /translation="MDRAKATTLTGAEIVVRCLQAEGVEYVFGYPGGAVLFIYDELFK
                     QDKVKHVLVRHEQGAVHAADGYSRSSKKVGVCLVTSGPGVTNAVTGIATAYMDSIPLV
                     VLTGQVPTHAIGLDAFQECDTVGITRPCVKHNFLVKDVKALASTLKKAFQIATTGRPG
                     PVLVDIPKDVTSQACEFDYPESVHMRSYNPVRKGHAGQIKKAVQLILEAERPMVYTGG
                     GVVLSNASAQLRELVKLLGFPCTNTLMGLGAYPATDPQFLGMLGMHGTYEANMAMQHC
                     DVLLAIGARFDDRVIGNPAHFAQNPRKIIHIDIDPSSISKRVKVDVPIVGNIPDVLDD
                     VLKLVKAARTKDLKPWWKQIGEWRAKDCLRYDRKSPIIKPQFVLEKLYEVTKGQAFVT
                     SDVGQHQMWAAQFYKFDQPRRWINSGGLGTMGFGLPAAMGVQLANPGATVACVTGESS
                     IQMCLQELSTCKQYRLPIKIINLNNKYMGMVRQWQQFFHGNRYSESYMDALPDFVKLA
                     EGYGHRGVLVEKPADVEPALREAFAAKQELVFLDFITDQKENVYPMIPGGKGITEMIL
                     YEDL"
     gene            7976..8554
                     /locus_tag="VFR57_04145"
     CDS             7976..8554
                     /locus_tag="VFR57_04145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0258599.1"
                     /translation="MASRSELAAFLQGVEKRAFKQAVFAVRDEEAALDIVQDAMLRLA
                     EKYGHLPVAELPALFHRIVQNAIRDWFRRQKVRSLWTTLLSSVGAGRDEDDEQDPLET
                     LQPAGRSYSQDAPAERLEQAQVLEIIEQEISRLPARQREAFMLRYWEELDVAETARAM
                     NCSEGSVKTHCSRAAHALQAALTARGITFETR"
     gene            8554..8934
                     /locus_tag="VFR57_04150"
     CDS             8554..8934
                     /locus_tag="VFR57_04150"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEU0258600.1"
                     /translation="MNEMQFGRRIRQILNQGTQVSPNIAERLTRSRELALARQRPEPA
                     PALAWADNALVRFGGWGGLALRVLLPLAMLVASGVAIYSWEQNQRAAELEEIDAQLLT
                     DDLPIDAYLDRGFQSWLKKRAAEQ"
     gene            8931..9410
                     /locus_tag="VFR57_04155"
     CDS             8931..9410
                     /locus_tag="VFR57_04155"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEU0258601.1"
                     /translation="MTRSVWLLLTALLSCGALAEPPVEVAPKAKPAVKAKPAAKAKAQ
                     VRPAWAELTAEQQQILAPLKADWESLEPERRRKWIGVAKRYPKMAPQGQERVQRRMQA
                     WAKLSPEQRRQARETYKQIVKVPPEKRGNLREQWAEYQQLPQHERDGLVSQGEKRRR"
     gene            9407..9805
                     /locus_tag="VFR57_04160"
     CDS             9407..9805
                     /locus_tag="VFR57_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0258602.1"
                     /translation="MSGATPGLPRRLASMVYEGVLLFAVAFFAAWLLFFASGGREATS
                     GGLRHALQLFILTMFAAYFLWCWLRGGQTLAMKAWHIRLVDVTPGKAVFRLILAGVMV
                     PTAISIVWALFDRERQFLHDRIAGTRLVDA"
     gene            complement(9816..>10628)
                     /gene="lptG"
                     /locus_tag="VFR57_04165"
     CDS             complement(9816..>10628)
                     /gene="lptG"
                     /locus_tag="VFR57_04165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU0258603.1"
                     /translation="TVMRASGLSPRRAAVTLAKIGLAFVVLTFLIGEWIAPWSEEQAQ
                     KVKLRAMSSAIGQDLQSGLWFKDEGSFINVREARQTNQLVGVRIYDFDADYRLQKITL
                     AQRADYAGGVWQLHDVATTRFTSEGPRIERQPLMEWRSRVNPDMLDALIVRPERMSAW
                     ALHKYTEHLAGNRQKTERYEIALWKKIFYPLAALVMMALALPFAYMQARAGMVGVKVF
                     LGIMLGISFHMLNSLFAHIGLLQNWPPLSAAVIPSAAFFAAAVLMMWWVERR"
BASE COUNT         1821 a         3476 c         3386 g         1945 t
ORIGIN      
        1 gagatctacc gcgagcccgc ctcgcccttc gtcgccgact tcgtcggcaa ggtgaacgtg
       61 ctcgccggga cggccgagaa cggcggcttg cgcatcggcg cggctgtttt ggaatgcaac
      121 ggagcccatg ttccgaaaag ccacagcgtc aagatctatc tccgccccga ggacatcgtg
      181 gcgcggccga tcgcgccggg cgactccaat gtgttcgacg cgcgtatcga gaaggtcgaa
      241 tttctgggct cgtactgcct ggtgcgcgtt gccaccgacg tcctcggcca gccgctcacg
      301 gtatatctct cgctcaattt cctgtccgag caggcgctcg cgccgggcag ccgcctgaaa
      361 ctgcgcatcc tgcccgaccg gctgcggctt ttcgatgcct gagatcgcag ttgcgatccg
      421 gcaaaggacc gatcccgccg accggatcgg ccatgccggg ctcctcgtcg tctttgtgct
      481 gttgatggcg tttctcgtgg cgccgctcgc cgccgtgctg atgcagagcg tggaagaccg
      541 gaatggccat ttcgtcgcgc tcgacaactt cgtcgcctat gtgcagacgc cggccctggt
      601 tcattcgctg tggaacagca tctgggtttc ggcggtggtg acgctgctca cgatcccggc
      661 ggcgttcctc ttcgcttacg ccctcacgcg cagctgcatg ccgctcaaag gcgtgttccg
      721 gaccatcgcg ctgaccccgc tgcttgcgcc ttcgctgctt gctgcgattt cgttcatcta
      781 ctggttcggc aaccagggcg tgctgaaaga cgcgatgcag gcgctcggca tcgcctccat
      841 ctacggcgcg gtggggatca tcgtgtcgga gatgttcgcg gtgtttccgc acgcgctcat
      901 gatcctgatc accgcgctga ctctagccga tgcgcgcctc tacgaggcag ctgacgcgct
      961 cggcacgagc gccgcgcgca agttcctaac catcacgttg ccgggcgcga aatacggcct
     1021 gatcagcgcg gcgcttgtca cgttcacgct ggtgatcacg gacttcggca tcccgaaggt
     1081 catcggcggc aacttcgacg tgctcgccac ggacatcttc aagcttgtga tcggccagca
     1141 ggacttccag cgcggggcgg tcgtcgcggt gctcctgctg gcgtccgctt tgatcacgtt
     1201 tttcgtcgat cgtctgattc agaggaagca gacatcgatg ctcaccgcgc gcgcggtccc
     1261 ctactccccc cggccctcgc cgggcttcga cctcgccatg acgctgtatt gcgcgctgat
     1321 cgcggcgctc atgctcgccg tgttcggcat ggcgctctat gcctcgttcg tgaagttctg
     1381 gccctacgat ctttcgttca gcctgcgcca ctacgccttc gggctggtgg acgcggaagt
     1441 cgacaccgcg ttcttcaaca gcctcaagct cgcgcttggc actgcgatcg gcggcacggt
     1501 gctggttttc gtgggcgcct acctgctgga gaaaacgcgc ggcgtagagt ggctgcggcc
     1561 ggggatgcag ctcctcgcgc ttgtgccgat ggcggtgccc ggtctggtgc tgggcctcgg
     1621 ctacatcttc tttttcaatt caccgggcaa cccgctcaac gggctgtacc acacgatgac
     1681 gctgctcgtg ctgtgcacca tcgtgcactt ctataccacc ggccacctca cggcggtgac
     1741 cgcgctcaag gcgctcgacg cggagttcga ggctgtgtcg gcgtcgctca aggtgccctt
     1801 ctaccgcacg ttcttgcgcg taacgctgcc gatttgcacg ccgacgcttc tcgacatcag
     1861 ccgctatttc ttcatcaacg ccatgaccac catctcggcg gtcgtgttcc tctactcgcc
     1921 cgataccaag gtcgcctcga tcgcgatcct caacctcgac gaggccggcg agcccggccc
     1981 ggcggctgcg atggcggtgc tgatcgcggc gacctcgctc gcagcctgcg tcgtgtacct
     2041 cggcctgggc tggctggtcg agcgcaagac ccagaaatgg agaaacctca agtgaaagag
     2101 ccgatcctgc tcacgcccgg accgctgact acctcgaccg agaccaagct cgccatggtg
     2161 cgcgattggg gctcgcgcga tacggggttc atcgagttga accgccgcgt gcgccaaagg
     2221 cttctggacg tggtcggtgt gagggagacc catgtctgcg tgccgctgca gggcagcggc
     2281 accttcgcgg tcgaggccat gctcggcacg atggtgccta agagcggcca cgtgctggtg
     2341 ccgctcaacg gcgcctattg caggcgcatc cagcgcatct gctcgatcct cggccggaaa
     2401 gtcactccca tcgaatacga cgagcgcgag ccggtagagc cggccgacat cgacgccgcg
     2461 ctcaaggcgg atccgacaat tagccatgtc gccctggtgc attgcgagac cagcaccggt
     2521 gtgctcaatc ctcttcatga aatctcggaa atcgtcgctc ggcatcgcaa gagcctgctc
     2581 gtcgacgcga tgagctcttt cgccgccgtc ccgatcgaag ggcccttcga tgcgctggtg
     2641 gcggcatcgg gcaagtgcct ggaagggccg ccgggaatgg gcttcgcgct gatgcgaaaa
     2701 agcgcgcttg agaagtgcac gggcaactcc ccctcgcttg tgctcgacct gcacgaccag
     2761 tgggtcaaca tggagaaaac cgcgcaatgg cgcttcaccc cgccgaccgt gatcgtcgcg
     2821 gcgcttcacg cggcgctcga gcagttcgtc gctgaaggcg gccgcgccgc gcgcggcgcg
     2881 cgctatggcc gcaattgcga ggtcctgatc gagggcatga cgaagctcgg cttcaagctg
     2941 ttcctcgacc gcaagcacca ggcgccggtg atcgtcactt tccacgcgcc gagcgacccg
     3001 aactacgatt tccagcgctt ttacgatcgc gtgcgcgaaa agggattcgt cctctatccc
     3061 ggcaagctca cccaggtcga cacgctgcgc gtgggctgca tcggcgcgat cgacgagcac
     3121 gcgatccgcg ccgctgtgca tgccatagcc gatacggtga cggagcaaag catccgcaac
     3181 tgcggcgttt cttcttctac tcctccggca gaacgggccg cacgcggtgg cccatggccc
     3241 acatgacgta gcccgacaag ccgtacgcca ggaagaagcc gaacagcacg agggacgggc
     3301 tgagcgaaac gatggagaag cccaccacca gggcgaggat cgcccagagc ggcacgcgtt
     3361 ttttccagtt cacttccttg aagctccaga acggaacgtt ggagaccatg gtgacgccca
     3421 cgaacaccgc aacggcccag gtgacggcgg cgatccagac gtgctcacgc ggcacgaagt
     3481 cgtggtcgac gagaacccag acgagtccgg ccatgatcgc ggcgccggag gggcttggca
     3541 gcccctggaa gtagcgcttg tcgaccacgg cgatgttggc gttgaagcgg gcgaggcgga
     3601 ttccagcgcc agcgcagtag ataaaggcgc cgatccagcc cgcggtgccg agctccttca
     3661 gcacccagac gtaggtcatg agcgccggcg ccgcaccgaa cgcgaccatg tcggcgatcg
     3721 agtcgtattg cgcgccgaac tcgctctggg tgttcgtcca gcgcgccacc cggccgtcca
     3781 ttccgtccag caccatggcg acgaagatcg cgatcgcgcc gatctcgaag cggccgttca
     3841 tcgcttgcac aatggcgaag aagccgcaga acagcacccc ggtggtgaag agattcggca
     3901 gcaagtagat cccccggcgt cgcagaccga tggggccggg ttgcgctact tcgggctcgt
     3961 agtcgggggc ttcagccatg cgagcacgct ctccgcggcg tacaccttgt cgccgatcgc
     4021 cgccttgacc tcgacgtcga gcggcaggta atggtcgacg cgcgagccga agcggatgaa
     4081 gccgaagcgc tggccgcgct tgagctcggc gccggtccca acatagcaca ggatgcggcg
     4141 cgcaatcagc cccgccacct gaacgcaggt gacgtcgtgg ccctccggcg tgtgcagcca
     4201 gagggcgttg cgttcgttct cgagcgaagc cttgtcgagg gcggcgttga cgaacgcgcc
     4261 ggggaagtac cagcgctcct tcaccacgcc gtcgaccggc gagcggttgg agtgcacgtt
     4321 gaacacgttc atgaacacgc tgaccttcag cgcgtcgcgc ttcacgtagg gatcctgcgc
     4381 gcggctcacc tcgaccacgc ggccgtcggc cgcggacagc accgcgcccg gggcgtcggg
     4441 cacctcgcgc ggcgggtcgc ggaagaactg caggatgaag atggcgccga gccagagcgg
     4501 gaccgaccac caccagccca gccagaagcc caccaggagg gagacggcga cagcgatgcc
     4561 aacgaacagc cacccttccc gggcgatcag cgggtgcggg tagcgccgtt gcttcaattc
     4621 ttcgaggtgt cgacgaggcg gttgcgtttg atccacggca tcatctcgcg cagtcgcgcg
     4681 ccgaccttct cgatcggcag ttccgccgtc atgcgccgct ggctgaggag gccaggcgcg
     4741 cccgcgcggt tctcgagcac gaattccttc gcgtactcgc cgttctggat acggtgcagc
     4801 gcctccttca tgcgcttcct cacaccctcg tcgatgatct tgggcccggt cacgtactcg
     4861 ccatattcgg cgttgttcga gatcgagtag ttcatggtgc cgatgccgcc ctcgtacatg
     4921 aggtcgacga tcagcttgag ctcgtgcagg cactcgaagt aggccatctc gggcgcgtag
     4981 cctgcgtcga ccagcgtgtc gaacccggcc ttgacgagct cgacgcagcc gccgcacagc
     5041 accgtctgct caccgaagag atcggtctcg gtctcctcgc ggaaagtggt ttcgatgatc
     5101 ccagccttgc cgccgccgat cgccgccgcg taggaaagcg cgacgtcgcg cgcttttttc
     5161 gacggattgc ggtgaaccgc aatcagcatt ggcactccgc cccccgcggc ataagtcgag
     5221 cgcacggtat ggccgggcgc cttcggcgcc accatccaca cgtcgaggtc ggcgcgcggc
     5281 tggatcagcc cgtagtggat gttgaaccca tgcgcgaacg cgagcgaggc gcccttcttg
     5341 atgttgggct cgacgaactg ctggtagacg gcggcgtggt gctcatcggg cagcaggatc
     5401 atcacgatat ccgcaccctt gatcgcgtcg gcgagctcgg ccaccttgac gccggccttc
     5461 ttcgccttgt cccacgaagc gccgcctttg cgcagcccga cggtgacctt gacccccgat
     5521 tcctgcaggt tcagagcatg cgcgtggccc tgcgagccgt agccgatgat ggtgaccttc
     5581 ttccccttga cgagcgaaag gtccgcgtcc ttgtcgtagt aaaccttcat gcatccccct
     5641 gaaaaactag acgcgcagaa cccgattgcc ccgcccgatt ccgctggccc cggttctcac
     5701 ggtctcgatg atggcgccct ggtcgagcga ccggatgaat gcgtcgagct tggcgccgtc
     5761 gccggtaagc tcgatggtat aggtgttgtc ggacacgtcg atgatgcggc cgcggaagat
     5821 gtcggccatg cgcttcatgt cctcgcgctc cttctggccg gctcggacct tgatcagcat
     5881 gagctcgcgc tcaatgtgct cggcctcgga gagatcgatg accttgacca cctcgatcag
     5941 cttgttcagc tgcttggtga tctgctcgac cacatcctcc gatccggtgg taacgatggt
     6001 catgcgcgac atcgacgcgt cttcggtcgg cgcgaccgtc agcgtctcga tgttgtagcc
     6061 gcgcgccgaa aagagcccgg cgatcctgga gagggcccct gcttcgttct ccaccaggat
     6121 cgagatgatg tggcgcatca gagatcctcg tagaggatca tttcggtgat ccccttgccg
     6181 ccgggaatca tcggatagac gttctctttc tgatcggtga tgaaatcaag gaacaccagc
     6241 tcctgcttcg cggcgaaagc ctcgcgcagc gccggctcca cgtccgcggg cttctccacc
     6301 agcacgccgc ggtggccata gccttcggcg agcttgacga agtcgggaag cgcgtccatg
     6361 tacgactcgg agtagcggtt gccgtggaag aactgctgcc actgacgcac cattcccatg
     6421 tacttgttgt tcaggttgat gatcttgatg ggcaggcggt actgcttgca ggtggaaagc
     6481 tcctgcaggc acatctggat cgaggactcg cccgtgacgc acgcaacggt cgcgcccggg
     6541 ttggcaagct gcacccccat cgccgccggc aggccgaagc ccatcgtgcc gaggccgccg
     6601 gagttgatcc agcgccgcgg ctggtcgaac ttgtagaact gcgccgccca catctggtgc
     6661 tggccgacgt ccgaggttac gaacgcctgg cctttggtga cctcgtagag cttctcgagc
     6721 acgaattgcg gcttgatgat cggggatttc cggtcgtagc gcaggcaatc cttcgcgcgc
     6781 cattcgccga tctgcttcca ccacggcttc agatccttgg tcctcgccgc tttgacgagc
     6841 ttcagcacat catccaggac gtcagggatg ttgccgacga tcggcacgtc gaccttcacg
     6901 cgcttggaaa tcgacgaggg gtcgatgtcg atatggatga tcttgcgcgg gttctgcgcg
     6961 aagtgcgccg ggttccctat cacccggtcg tcgaagcgcg ctccgatcgc gagcagcacg
     7021 tcgcagtgct gcattgccat attggcttcg taggtgccgt gcatgcccag catgccgagg
     7081 aactgcgggt cggtggccgg gtaggcaccc aggcccatca gcgtgttggt gcaggggaaa
     7141 ccgagaagct ttaccagctc gcgcagctgg gcggaagcgt tcgacaggac gaccccaccc
     7201 ccggtgtaga ccatcggccg ctcggcctcg aggatgagct gcaccgcctt cttgatctgc
     7261 ccggcgtggc ccttgcgcac cgggttgtag gagcgcatgt gcacgctctc cgggtagtcg
     7321 aactcgcagg cctgactcgt cacgtccttg gggatgtcga cgagcaccgg cccgggacgt
     7381 ccggtggtcg cgatttgaaa ggccttcttg agagttgaag cgagggcctt cacgtccttc
     7441 accaggaagt tatgttttac gcacggccgg gtaatgccga ccgtgtcgca ctcctgaaag
     7501 gcgtccagcc cgatcgcgtg ggtcggcacc tggccggtga ggaccacgag cggaatcgaa
     7561 tccatatacg cggtggcaat accggtgacc gcgttcgtga cgccaggccc cgacgtcacc
     7621 agacagacgc cgactttctt ggacgagcgc gagtagccgt cggcggcatg caccgcaccc
     7681 tgctcgtgcc gcaccaggac gtgcttcacc ttgtcctgct tgaacagctc gtcgtagatg
     7741 aataggaccg cgccgccggg gtagccgaag acatactcga cgccctcggc ctgcaggcag
     7801 cgcacgacga tttctgcgcc cgtcagcgtg gtggctttcg cccgatccat ctaagctcgt
     7861 gtaagtatcg gaaaagtctt ggacaataaa ggcttggtgc ggtgcggtca agccgcgccc
     7921 ggcagcgcgc gccggccttt tgataccatc ggccgccaac aacagagggg ccaccctggc
     7981 atcccgaagc gagcttgccg ctttcctgca aggcgttgag aagcgcgcgt tcaagcaagc
     8041 cgtgttcgcc gttcgcgacg aggaggcggc acttgacatc gtgcaggacg cgatgctgcg
     8101 gcttgctgag aaatatggcc accttcctgt ggccgagcta ccggcgcttt tccatcgaat
     8161 tgtgcagaac gcgattcgcg actggttcag gcgccaaaag gtgcgctctc tgtggaccac
     8221 gctcctttcc tccgtaggcg ccggtcgtga cgaagacgac gagcaggacc cgctggaaac
     8281 cctgcagccc gccgggcggt cgtattccca ggatgccccc gcggagcgcc tggagcaggc
     8341 ccaagtactt gaaattattg agcaagagat ttcgcggttg cccgcgcgtc aacgggaagc
     8401 tttcatgctg cgttattggg aggaactcga cgtggccgaa acggctcggg caatgaactg
     8461 ctcggagggc agcgtgaaaa cgcattgctc ccgggccgcc catgctttgc aggcggcgct
     8521 cacggcgcgc ggtatcacgt tcgagacaag atgatgaacg aaatgcaatt cggccgcagg
     8581 atccgtcaga tcctgaatca ggggacccag gtcagtccga acatcgcgga gcgcctgacg
     8641 cgctcccgcg agctggcgct ggcacggcag cgcccggagc cggcgcccgc attagcctgg
     8701 gcagacaacg ccctcgttcg cttcggcggc tggggcgggc tggcgttgcg cgtactgctg
     8761 ccactcgcaa tgctcgttgc gagcggcgtt gccatttaca gctgggagca aaaccagcgc
     8821 gccgcggagc tggaagagat cgacgcccag ctcctgaccg acgacctgcc gatcgacgcc
     8881 tacctcgatc ggggcttcca gagctggctc aagaaacgcg cggcggagca atgacgcgct
     8941 ctgtctggct tctgctcacc gcgctgctga gctgcggcgc gcttgccgag cccccggtcg
     9001 aggtcgcgcc caaggcgaag cccgccgtga aggcgaagcc ggcggccaag gccaaggcgc
     9061 aggtgcgccc ggcctgggcg gaactcactg ccgagcagca gcagatcctt gcccccctga
     9121 aagccgattg ggaaagcctg gagcccgagc ggcggcggaa atggatcggc gtcgccaagc
     9181 gctatccgaa gatggcgccg cagggccaag agcgagtgca gcgacgtatg caggcatggg
     9241 ccaagctctc gcctgagcag cggcgccagg cgcgcgagac gtacaagcag atcgtaaagg
     9301 tgccgcccga gaagcgcggc aacctgcgcg agcagtgggc cgagtaccag cagctgccgc
     9361 agcatgagcg cgacggcctg gtttcccagg gagaaaagcg acgccgttga gcggcgcgac
     9421 gccgggcttg ccgcgccgcc tggcgagcat ggtctacgag ggggtcctgc tcttcgccgt
     9481 cgctttcttc gccgcgtggc tactcttctt cgcgagcggc ggccgcgagg cgacttccgg
     9541 cgggttgcgc catgcgctcc agctcttcat cctgacgatg ttcgcggcct acttcctttg
     9601 gtgctggcta cgcggcgggc agacgcttgc gatgaaggcc tggcacatcc ggcttgtcga
     9661 cgtaacgccc ggcaaggcgg tcttccgctt gattctggcc ggcgtgatgg ttccaaccgc
     9721 gatctccatc gtatgggcgc tgttcgatcg agagcgccaa ttcctgcacg accggatcgc
     9781 cggcacccgc ctggtcgacg cctgagcggc gccgtctacc ttctttcgac ccaccacatc
     9841 atgaggaccg cggcggcaaa aaacgcggcg ctcgggatta ccgctgcgga gagcggcggc
     9901 cagttctgca gcaggccgat atgggcaaag aggctgttca gcatgtggaa ggaaataccg
     9961 agcatgatgc cgaggaatac ctttacccct accatgcctg cgcgcgcctg catgtaggcg
    10021 aacggaagcg cgagcgccat cattaccagc gccgccagcg ggtagaaaat cttcttccat
    10081 agcgcgatct cgtagcgttc cgtcttctgc cggttgcccg cgaggtgctc ggtgtacttg
    10141 tgcagcgccc aggccgacat gcgctcgggg cgcacgatca gcgcatcgag catgtcggga
    10201 ttcacgcgcg agcgccactc catgagcggc tggcgctcga tgcgcggccc ctcgctggta
    10261 aagcgggtgg tcgccacgtc gtgcagctgc cagacgccgc cggcatagtc cgcgcgctgc
    10321 gcaagcgtga ttttctgcag gcggtagtcg gcgtcgaaat cgtagatgcg tacgcctacg
    10381 agctggtttg tctgccgcgc ttcgcgcacg ttgatgaacg agccctcgtc cttgaaccac
    10441 aggcccgact gcaggtcctg accgatggcc gagctcatcg cgcgcagctt caccttctgc
    10501 gcctgctcct ccgaccaggg agcgatccat tcgccgatga gaaaggtgag cacgacgaaa
    10561 gccagcccga tcttggcgag cgtgaccgcg gcgcgccgag gcgaaagtcc ggaagcgcgc
    10621 attacggt
//
LOCUS       DASTPD010000119         8299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1286420, whole genome shotgun sequence.
ACCESSION   DASTPD010000119 DASTPD010000000
VERSION     DASTPD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286420"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFR57_04170"
     CDS             <1..1091
                     /locus_tag="VFR57_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0258604.1"
                     /translation="LLGCSERDAARKPLAAPSGDAGAGKLFAERECKGCHGLDGKGAA
                     PAIPHLAAQRESYLLASLRAYKEGKRTHAALKEIATQMSDADARNIAAYYAGLPPVQA
                     ATGETKTVSPYERGQSLAAACAKCHGEDGNSTTPGIPTLAGQQPHYLVVAIQEYLASE
                     RKAAPMHALLPSLSRLDKESLALYFASQTPQARAAPSFGDPAAGEPLTAVCGGCHGSH
                     GVSIDTATPILAGQDAQYLVQAIKAYRTTRQRETMRAYVQKLSDKEIENIAAFYSVQK
                     SRPAEKGQTLVQELTDKCNRCHGAQVDNPSAPIPKIGGQDRDYLIMALRAYRDDRRES
                     TTMHKMSLPYGDAVIDSIASYYAAQPAK"
     gene            complement(1093..1701)
                     /locus_tag="VFR57_04175"
     CDS             complement(1093..1701)
                     /locus_tag="VFR57_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0258605.1"
                     /translation="MILRIASLVLAGCAVLALSAQAAAQKSGKEVVESTCVSCHGTGK
                     GGAPKIGDKKAWSKRSASGLSSLTQSALKGIRNMPAHGGNAGLSDLELARGIAYMVNE
                     SGGKWTEPASAGELKAERTGAQVVKMQCVKCHQDGKGGAPKIGDRDAWSKRLSQGVDH
                     AVHSAIKGHGGMPPRGDRADLTDGEVRAAILYMFNAKAPAKK"
     gene            complement(1706..1984)
                     /locus_tag="VFR57_04180"
     CDS             complement(1706..1984)
                     /locus_tag="VFR57_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEU0258606.1"
                     /translation="MKATVLVFFAAATLAAGAASAQSGAEVLKTKGCNNCHESDKKKV
                     GPAYKDIAKKKGQEAALTAKLKEGKSHPKVAVSDAELKAALDHIMAMK"
     gene            complement(2071..4203)
                     /locus_tag="VFR57_04185"
     CDS             complement(2071..4203)
                     /locus_tag="VFR57_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEU0258607.1"
                     /translation="MRKTTLSALVAGLFASPALAQQAEFTGSLSITGYVNDVDSRNPY
                     LFEKYRDLSDGITGGTDLWRHDEGGRMRLYSENLGRDDQFIEFSGQRYGVLKYSLYND
                     KVVHNLTFGAITPYIGVGTNNLTYPGAAGATPSTNIATWGGFDYEKKHDNYGGTLEVK
                     PWATPWYLRATANQKKTEGLLPTGNAMTSPGGPFVELPMPVHWTTTDVSAEAGYSTKA
                     SHYSLLFSWSRFEDDNQYYFWRNPAITGSNTVLERSTIAPSNELWRVVGNAVWKQLPL
                     ASTLALRGTYGVGTSEVPIQTSFPLTPTGAPRAANPNATTFDGEVTYKTLSASLNSHL
                     ARSLDSRIYFNWNKRENESTVVVFTPTGGNACDIDPVTGVGLTTCTNDPFHYERRNFG
                     VDLQYRLSRQNKFSAGWDWSDIERERHDFHESEENKLYVEWKNNSLEDLTARIKYQFL
                     QRDSEFGFGNINPFANNTNLFRYHLRPFDANDLDQNLVKLALDWNPQRLLDVGAELIY
                     KDNNYKNAELGRTGDTRRELYLSAAYGDPKAFRVSTFFDFETIRYDSTHYQGAPVPLS
                     SFPASASPTAFQWKSQVEDTNYVIGLAADWLPLARLRFRGSYIYQETEGTVDFSANNP
                     AAVIRNIPNYDSFTKHTFDLRAIYDVTKTIEVTIGAAYEKYRYDDAQFNNYNYIQVAG
                     GQNFLSGAYAFPDYEALLGYLTLKYHFR"
     gene            complement(4213..5166)
                     /locus_tag="VFR57_04190"
     CDS             complement(4213..5166)
                     /locus_tag="VFR57_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEU0258608.1"
                     /translation="MLTRLVAALPAALFALASAPAIAQPPGPEVCAGCHAEYVETFAA
                     SIHGQKGHPKSPASAGGCVACHGDGTEHVKAGGGRGVGGIRALSPRSKALSGEQKSEI
                     CLTCHADNRHLAFWDAGRHRKNDVTCSNCHSTHGGNDQLLRVENPPIQPFVTTSRVPQ
                     EEVCFTCHRDIRAQVLRPSHHPIQEGKIKCASCHNPHGALSHAMLRHETVNEQCYSCH
                     ADKRGPFLFEHPPVQENCLTCHNPHGSRSMRLLREKPPNLCQDCHDAAQHPGTAYDLT
                     EAFPPIGTAPSTRFIARSCLNCHQHIHGSNAPASKGRRFVR"
     gene            5417..6058
                     /locus_tag="VFR57_04195"
     CDS             5417..6058
                     /locus_tag="VFR57_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0258609.1"
                     /translation="MPRLRIFWGVPAASLSPLTKQCLVLSVARGTNVVQRGARLPGVF
                     ALAYGSVKLSLRGDDAEERVVSLVSPGQTFGEAKALLGCASRSDAIALVDTKLIVIPS
                     AAILSLMDREPRFARAMVFVLAERTLDLLGELESATQRGAHRLAAYIVALADGNGDTV
                     QLPVSKTLVAARLGVKKETLSRLLRSLETRGLITVERREVRILDRTGLAAVTR"
     gene            complement(6055..6645)
                     /locus_tag="VFR57_04200"
     CDS             complement(6055..6645)
                     /locus_tag="VFR57_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0258610.1"
                     /translation="MKNAVTILKSEHRSISAVVQALKALARMAQDPKVQPDWRPFRAI
                     LRYIDEYPERLHHPKEDKFLFARLGERCTDARKLIEDLKGEHVQGARLIRELERVFVF
                     FEERWPQGGREFLAAVDDYAEFHWAHMRKEEQQLLPLAERHFTPEDWEAVDNAFAANT
                     DPLQSIKERDFDSLLSRIANLAPAPVGLGEPWKKAN"
     gene            complement(6642..7139)
                     /locus_tag="VFR57_04205"
     CDS             complement(6642..7139)
                     /locus_tag="VFR57_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEU0258611.1"
                     /translation="MKTVAHLYAHAIAIEREAAERYSEFAERMDDLGNDAAAEVFGTL
                     ARLEAEHLDELLKRTAGMALPGLAAHEYAWLDSGAPETAARELVFRLMTPRHALEIAL
                     AGEKRAAAFFESVVMRADDAMLRALATEMAADETDHIAMIEKLLEKTPSAIVDWASAY
                     EGETP"
     gene            complement(7196..>8299)
                     /locus_tag="VFR57_04210"
     CDS             complement(7196..>8299)
                     /locus_tag="VFR57_04210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU0258612.1"
                     /translation="AEHKTFIQSVPYMDRLDYVSMMCNEHAYVLAIEKLLGVQVPLRA
                     QYIRVMFDEITRILNHLLFLGTQGLDLGAMTIFLYCFREREDLLDCYEAVSGARMHAA
                     YYRPGGVYRDLPDSMPQYEPSRFRSEKDLKALNRNRQGSLLDFIEDFTERFPKCVDDY
                     EGLLTENRIWKQRTVGIGVVSPERAKALGFSGPMLRGSGIAWDLRKKQPYEVYDRMEF
                     DIAIGKNGDTYDRYLVRVEEMRQSNRIIKQCVKWLRENPGPVIVDDHKVAPPSRAEMK
                     LNMEELIHHFKLFTEGMHVPEGEAYAAIEHPKGEFGVYFISDGANKPYRMKLRSPAFC
                     HLSAMNELAKGHMLADAVAIIGTLDIVFGDVDR"
BASE COUNT         1327 a         2643 c         2779 g         1550 t
ORIGIN      
        1 tattgctggg ttgttcggaa agggacgcgg cgcgcaagcc gcttgccgcg ccttcgggcg
       61 acgccggcgc cggcaagctc ttcgccgagc gtgaatgcaa gggctgccac ggcctcgacg
      121 gcaagggcgc cgcgccggca attccgcacc tcgccgcgca gcgggagagc tatctgcttg
      181 cgtcgctgcg cgcgtataag gaaggcaagc gcacccatgc cgcgctcaag gaaatcgcga
      241 cccagatgag cgatgccgat gcgcgcaaca ttgccgctta ctatgccggc ttgccgccgg
      301 tacaggcagc gacgggcgag accaagactg tgtcgccgta cgaacgcggg caatcgcttg
      361 ccgccgcctg cgccaagtgc catggcgagg acggcaacag caccacgccg gggattccca
      421 ccttggccgg ccagcagccg cactacctcg tggtcgcgat ccaggaatat ctcgcgagcg
      481 agcgcaaggc cgcgccgatg cacgccctcc tccccagctt gagcaggttg gacaaggaaa
      541 gcctggcgct ctatttcgcc tcgcagacgc cgcaggcgcg cgctgcgccg tccttcggcg
      601 atcccgcggc aggcgagccg ctcaccgcag tgtgcggcgg ctgccacggc tcgcacggcg
      661 tgagcatcga cacggcgacg ccgatcctcg ccggccagga cgcgcaatat ctcgtgcaag
      721 cgatcaaggc ctaccgcacc acgcgccagc gcgaaacgat gcgcgcctac gtgcagaaac
      781 tgagcgacaa ggagatcgaa aacatcgcgg ccttctattc agtgcagaaa tcgaggcccg
      841 cggaaaaggg ccagacgctg gtgcaggagc tcaccgacaa gtgcaatcgc tgccacggcg
      901 cgcaagtcga caacccctcg gcgcccatcc cgaagatcgg cggacaggat cgcgactacc
      961 tgatcatggc gctcagggcc taccgcgacg accggcgtga gagcacgacg atgcacaaaa
     1021 tgagcctgcc gtacggcgac gcggtgatcg acagcatcgc ctcctactac gccgcccagc
     1081 cggccaagtg agctatttct tggccggcgc tttcgcgttg aacatgtaaa ggatcgccgc
     1141 gcgcacctcg ccgtcggtga ggtccgctcg gtcgccgcgc ggcggcatgc cgccgtggcc
     1201 cttgatggcc gagtgcaccg cgtggtcgac gccctgcgag aggcgcttgc tccaggcgtc
     1261 gcggtccccg atcttcggcg caccgccctt accgtcctgg tggcacttga cgcactgcat
     1321 cttcaccacc tgcgctccgg tgcgctcggc cttgagctcg cccgcgctcg caggctcggt
     1381 ccacttgccg cccgactcgt tgaccatgta ggcgatgccg cgcgcgagct cgaggtccga
     1441 aaggcccgcg ttgccgccgt gcgccggcat gttgcggatg cccttgagcg cgctctgcgt
     1501 gaggctcgaa agccctgacg ccgagcgctt gctccaggcc ttcttgtcgc cgatcttggg
     1561 ggcgccgccc ttgccggtgc catggcaact gacgcaggtc gattcgacga cttccttgcc
     1621 gctcttctgc gccgcggcct gcgcggacag tgccaggacg gcgcagccgg cgagcacgag
     1681 cgatgcgatg cgcaggatca aggccttact tcatcgccat gatgtggtcg agcgcggctt
     1741 tcagctcggc gtcggagacg gcgactttcg gatggctctt gccttccttg agcttcgcgg
     1801 tgagggccgc ctcctggccc ttcttcttcg cgatgtcctt gtacgcaggc ccgaccttct
     1861 tcttgtcgga ctcatggcag ttgttgcagc ccttcgtttt cagcacctcg gcgcccgatt
     1921 gcgcgctggc ggcgcccgcg gccagcgtgg cggcggcaaa aaatacaagc acggttgctt
     1981 tcattgcggt ttctcccgtg gaggacagtg gagcgattgt ataacgcacc gccgcccggc
     2041 tcggtttacc tgccgggcga gcgaatgcgg ttagcggaag tgatacttca gggtcagata
     2101 gccgagcaac gcctcgtagt ccgggaacgc gtaggcgccg ctcaggaaat tctggccgcc
     2161 ggcaacctga atgtagttgt agttgttgaa ctgcgcgtcg tcgtaccggt atttctcgta
     2221 cgccgcgccg atcgtcacct cgatggtctt ggtcacgtca tagatggcgc gcaggtcgaa
     2281 ggtgtgcttc gtaaagctgt cgtagttcgg aatattgcgg atcaccgctg ccgggttgtt
     2341 ggcggagaag tcgaccgtgc cctcggtctc ctggtagatg taggagccgc ggaagcgcag
     2401 ccgcgcgagc ggcagccagt cggcggccaa gccgatgacg tagttcgtgt cttcgacctg
     2461 cgatttccac tggaacgcag tcgggctggc cgaggccggg aatgaggaaa gcgggaccgg
     2521 agcgccctgg taatgcgtcg agtcgtagcg gatggtctcg aagtcgaaga aggtgctgac
     2581 ccggaatgcc ttcggatcgc cgtacgccgc gctgagatac agctcgcggc gcgtgtcgcc
     2641 ggtgcgtccg agctccgcgt tcttatagtt gttgtccttg tagatcagct cggcgccgac
     2701 atcgagcagg cgctgcggat tccagtcgag cgccagcttg acgaggttct ggtcgaggtc
     2761 gttggcgtcg aacggccgca ggtggtagcg aaacaggttg gtgttgttgg cgaaaggatt
     2821 gatgttgccg aaaccgaact ccgaatccct ctgcaggaac tggtacttga tgcgcgcggt
     2881 gaggtcttcg agcgaattat tcttccactc gacgtagagc ttgttctcct cgctctcgtg
     2941 gaagtcatgc cgctcgcgct cgatgtcgga ccagtcccag cccgccgaga acttgttttg
     3001 ccggctgagg cgatactgga ggtcgacacc gaaattgcgc cgctcgtagt ggaacgggtc
     3061 gttggtgcac gtggtgagcc ctacgccggt taccgggtcg atgtcgcacg cattgccgcc
     3121 cgtcggagtg aacaccacca ccgtggactc gttctcgcgc ttgttccagt tgaagtagat
     3181 gcgcgagtcg agcgagcgcg cgagatgcga gttgagcgaa gcggaaagcg tcttgtaggt
     3241 cacctcgccg tcgaaggtcg tcgcgttcgg attggccgcg cgcggggcgc cggtcggtgt
     3301 cagcggaaac gaggtctgga tcggtacctc gctcgtgccg acgccatagg tgccgcgcag
     3361 cgcaagggtc gaagcgagcg gcagctgctt ccaaaccgcg ttgccgacca cccgccacaa
     3421 ttcgttgctg ggcgcaatcg tgctcctctc gagcaccgtg ttgctgccgg tgatcgccgg
     3481 gttgcgccag aagtagtact ggttgtcgtc ttcgaacctg ctccacgaga acagcagcga
     3541 atagtggctg gcctttgtgg aatagccggc ctccgccgag acgtcggtgg tggtccaatg
     3601 gaccggcatc ggcagctcca cgaatggccc gcccgggctc gtcatggcgt tgcccgtcgg
     3661 cagcaggccc tcggtcttct tctggttagc ggtggcgcgc aagtaccacg gcgtcgccca
     3721 cggcttgacc tccaacgtac cgccgtagtt gtcgtgcttc ttctcgtagt cgaagccgcc
     3781 ccaggtcgcg atgttggtcg acggcgtcgc gcccgccgcg cccgggtagg tgagattgtt
     3841 cgtccccaca ccgatatagg gcgtgatcgc gccgaaggtc aggttgtgga cgaccttgtc
     3901 gttgtagagc gagtatttca gtacgccata gcgctgcccc gagaactcga tgaactggtc
     3961 gtcgcgcccg aggttttcgg agtacagccg catgcgcccg ccttcgtcgt ggcgccacag
     4021 atcagtgccc cccgtgatgc cgtcgctcag atcgcggtat ttctcgaaca ggtacggatt
     4081 gcggctgtcg acgtcgttga cgtagcccgt gatgctcaac gagccggtga actcagcctg
     4141 ctgcgcgagc gcgggagacg cgaacagccc agcgacgaga gcggaaaggg tggtttttct
     4201 catgttcccc tcctatcgca cgaagcgccg gcccttcgaa gcgggcgcat tgctgccgtg
     4261 gatgtgctgg tggcagttga ggcagctcct cgcgatgaag cgcgtgctcg gcgcggtccc
     4321 gatgggagga aacgcctccg tgaggtcgta agcggtgccc gggtgctgcg cggcgtcgtg
     4381 gcagtcctgg cagaggttgg gcggcttctc gcgcaacagg cgcatcgagc ggctgccgtg
     4441 cgggttgtgg caggtgaggc agttctcctg gactggcgga tgctcgaaaa ggaaggggcc
     4501 gcgcttatcg gcgtggcagc tgtagcactg ttcgttcacc gtctcgtgcc gcagcatcgc
     4561 gtggctgagc gcgccgtgcg ggttgtggca gctcgcacac ttgatcttgc cctcctggat
     4621 cgggtggtgc gagggcctga gcacctgcgc gcggatgtcg cggtggcagg taaagcagac
     4681 ttcctcttgc ggcacgcgcg aggtggtgac gaagggctgg atcggcgggt tttccaccct
     4741 caggagctgg tcgttaccgc cgtgggtgct gtggcaattg ctgcacgtga cgtcgttctt
     4801 gcgatggcgc ccggcatccc agaaggcgag atggcggttg tcggcgtgac atgtgaggca
     4861 gatctcgctc ttctgctccc cggagagggc tttgctgcgc gggctgagcg cccggatgcc
     4921 gccgacgccg cggccgccgc cggccttcac gtgctcggta ccatcgccat ggcaggcgac
     4981 gcagccaccg gcgctcgccg gactcttggg atgtcccttc tggccgtgga tcgaggccgc
     5041 gaaggtctcg acgtactccg cgtgacagcc cgcgcagacc tcggggccgg gcggttgcgc
     5101 aatcgccggc gcacttgcaa gcgcgaagag agcggcgggc aaagccgcaa cgagcctcgt
     5161 cagcatttcg gtcctcccag gaaaccgaac acggtgcttg agcgatgatg cccacgccgc
     5221 tgcgattggg cttgcgaaat cgcgcaggcc cgttgaccta aatcaaacgg gccggatact
     5281 ttgcggacat ccttccatcg ttgatcttca tcaacgatgc aacgctgctg caacgcagca
     5341 tgagggtgaa aggaacgcga tgtcgccaaa atcggctgcg aatgcggccc tcgaacagcg
     5401 cctgattcaa ggcctcgtgc ctcgactgcg catcttctgg ggcgtccccg ccgcgagcct
     5461 gagcccgctc acgaagcagt gcttggtgct aagcgtggcg cgcggcacga atgtcgttca
     5521 acgcggcgcg cgcctgcccg gtgtcttcgc ccttgcctac ggctcggtca agctcagcct
     5581 gcgcggcgac gacgccgagg agcgcgtcgt ctccctcgtg tcgccgggcc agaccttcgg
     5641 cgaggccaaa gcgcttctcg gttgtgcctc gcgcagcgac gcgatcgcgc ttgtcgatac
     5701 gaagctcatc gtcatcccct cggctgcgat cctgagcctg atggaccgcg agccgcgctt
     5761 cgcgcgcgcc atggtcttcg ttctagcgga acgtaccttg gaccttctcg gagagctgga
     5821 atcggcgacc cagcgcggcg cgcaccgcct ggcggcctac atcgtggcgc tcgccgacgg
     5881 gaacggcgat accgtgcagc ttccggtcag caagacgctg gtggccgcgc ggctgggcgt
     5941 gaagaaggaa acgctgtcgc ggctattgcg cagcctcgaa acacgcggcc tgatcacggt
     6001 cgagcggcgc gaggtgagga tcctcgaccg caccgggctc gccgcggtga cgcgctaatt
     6061 tgcttttttc cagggctcgc ccagcccgac cggcgcgggc gcgaggttgg cgatgcggct
     6121 gaggaggctg tcgaagtcgc gctctttgat gctttggagc ggatccgtgt tcgccgcgaa
     6181 cgcgttatcg accgcctccc agtcctcggg cgtgaagtgg cgctcggcaa gcggcagcag
     6241 ctgctgctcc tccttgcgca tgtgcgccca gtggaactcg gcgtagtcgt cgaccgcggc
     6301 gaggaattcg cgcccgccct gcggccagcg ctcctcgaag aacacgaaca cgcgctcgag
     6361 ctcgcggatc agccgcgcgc cctgcacatg ctcgcctttc agatcctcga tcagcttgcg
     6421 cgcgtcggtg cagcgctcgc cgagccgcgc gaacaggaac ttgtcctcct tcggatgatg
     6481 caggcgctcg gggtactcgt cgatgtagcg caagatggcg cggaaagggc gccagtcggg
     6541 ctgcaccttg ggatcctgcg ccatgcgcgc gagcgccttc agcgcctgca ccacggcgga
     6601 gatcgagcga tgctcgctct tgaggatggt gaccgcgttc ttcacggcgt ttcgccttcg
     6661 taggccgagg cccagtcgac gatcgcgctc ggcgtctttt ccagcagctt ctcgatcatc
     6721 gcgatgtggt cggtttcgtc ggccgccatc tcggtggcca gcgcgcgcag catcgcatcg
     6781 tcggcgcgca tcacgacaga ctcgaagaac gcggccgcgc gcttctcgcc cgcgagcgcg
     6841 atctcgagcg cgtggcgcgg cgtcatcagc cggaacacga gctcgcgcgc cgcggtctcg
     6901 ggcgcgccgc tgtcgagcca ggcatattcg tgcgccgcca gccccggcag cgccatgccg
     6961 gccgtgcgct tgaggagctc gtcaagatgc tcggcttcga ggcgcgcgag ggtgccgaat
     7021 acctcggccg cagcgtcgtt gcccagatcg tccatgcgct cggcgaactc ggaatagcgc
     7081 tccgccgctt cgcgctcgat cgcaatcgcg tgggcgtaga ggtgggcgac ggttttcatg
     7141 ccgccacgat gcgccgtccg gcgcggcgcg gcttgatctt tatcaaagag ctgtcttacc
     7201 ggtcgacgtc gccgaaaacg atgtcgagcg tgccgatgat cgccacggcg tcggcgagca
     7261 tgtggccctt ggcgagctcg ttcatcgccg aaagatggca gaacgccggc gagcgcagct
     7321 tcatgcggta gggcttgttg gcgccgtcgg agatgaaata cacgccgaac tcgcccttcg
     7381 gatgctcgat cgccgcgtag gcctcgcctt cgggcacgtg catgccctcg gtgaagagct
     7441 tgaagtggtg gatgagctct tccatgttga gcttcatttc cgcgcgcgaa ggcggcgcaa
     7501 ccttgtggtc gtcgacgatg actggcccgg gattttcccg cagccacttc acgcactgct
     7561 tgatgatgcg gttcgactgg cgcatctcct cgacacgcac gaggtaacga tcgtacgtgt
     7621 cgccgttctt gccgatcgcg atgtcgaatt ccatccggtc gtagacctcg taaggctgct
     7681 tcttgcgcag gtcccaggcg atccccgagc cgcgcagcat ggggccgctg aagccgagcg
     7741 ccttggcgcg ctcgggcgac actacgccga tgccgacggt gcgctgtttc cagatgcggt
     7801 tctcggtgag cagtccctcg tagtcgtcga cgcatttcgg gaagcgctcg gtgaagtcct
     7861 cgatgaagtc cagcagcgac ccctggcggt tgcggttcag cgccttcagg tctttctcgc
     7921 tcctgaacct ggagggttcg tattgcggca tcgagtcggg caagtcccgg tacacgccgc
     7981 cgggtcggta gtacgccgca tgcatgcgcg ccccggacac cgcctcgtag cagtcgagca
     8041 ggtcctcgcg ctcgcggaag cagtagagga agatggtcat cgcgccgagg tcgaggccct
     8101 gcgtgccgag gaagagcaga tggttgagga tgcgggtgat ctcgtcgaac atgacgcgga
     8161 tgtactgcgc gcgcagcggc acctgcacgc cgagcagctt ctcgatggcg agcacgtagg
     8221 cgtgctcgtt gcacatcata gagacgtagt cgagccggtc catgtagggc accgactgga
     8281 tgaaggtctt gtgctcggc
//
LOCUS       DASTPD010000120        13484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1286821, whole genome shotgun sequence.
ACCESSION   DASTPD010000120 DASTPD010000000
VERSION     DASTPD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286821"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFR57_04215"
     CDS             complement(<1..790)
                     /locus_tag="VFR57_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258613.1"
                     /translation="MFQVLLTRVFSLTMWYHFAFMAISIAMFGMTIGALAVFLRPAWW
                     PAERLASAMGRCAVLCSISMVAVTFLHISLYVPKPGEYIWPVIVTFVCAAVPFVFAGI
                     FVCLALTRFPQSVGKLYAADLAGAAVGCLGVIAALRWLDGVGAVLAAATLAALAGIFL
                     LRGKGRALAAAVTAALAGTTLWSGLYLDRHGISPFKIQSAFSGFDVEYERWNSFSRIT
                     VLKAEAQHWSLSSAFQGSPKVPTRLLHIDAIAGTQLIGFDGDLKK"
     gene            1239..1373
                     /locus_tag="VFR57_04220"
     CDS             1239..1373
                     /locus_tag="VFR57_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258614.1"
                     /translation="MLLRELHRMGPARGLCAVYLARLEDSRLHPPPPEWNGVTAFDEK
                     "
     gene            1387..2154
                     /locus_tag="VFR57_04225"
     CDS             1387..2154
                     /locus_tag="VFR57_04225"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEU0258615.1"
                     /translation="MRLRVLGCSGGIGGRHLRTTSFLLDSDILIDAGTGVGDLTLAEL
                     SQIDHIFITHSHLDHVTSIPFLVDTVGGMRSRPLNVYATRATIEILKNHLFNWAIWPD
                     FTEIPSLEAPFMRYLEIELGTAVTLGGRTITAIPARHTVPAVGYHLDSGAASLVFTGD
                     TGPNDTLWRIVNRIANLKFLIIETAFSNKERQLAEISRHLCPATLAEELAKLERDPEI
                     YITHLKPGEIEPTMLEIEECAGDFKPRMLQNNLVLEF"
     gene            2163..3203
                     /locus_tag="VFR57_04230"
     CDS             2163..3203
                     /locus_tag="VFR57_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0258616.1"
                     /translation="MNGSLRPTRDQLAMLVPLAGLSRERLAELAEATLIERAARGADL
                     LAGRARPGPSLFLLAGEVLLGFEAGGTLVVVGGTEETRQALNRRKERVARARAITDVD
                     LLVVEDELLDVLATWDQVAAARAPDAVASGAFSLSSLRAGVFAQLPPAHIDELLRRFE
                     RVNAARGEVVIREGDEGDYYYVIQSGRFQVERLVGGADVVLAELKVGDAFGEEALVAE
                     AKRNATVTALTDGQLLRLNRPDFIELLREPLLRRIGFEEASAKVRRGALWLDVRYPSE
                     YQYDKLPGAINVPLAEVRNMFAVLDRTKEYIAYCQSGRRSAAAAFLFAQRGFKVWLLE
                     HGLKGVEKPESR"
     gene            3248..5620
                     /locus_tag="VFR57_04235"
     CDS             3248..5620
                     /locus_tag="VFR57_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU0258617.1"
                     /translation="MSAVLSPHASASSDVGARLAFQKQLQAVTNKIHATSNIDEIMLE
                     VSADVCVLFNADRLTIYSVGEDRQSIVSKVKTGLNSFKDLKLPIAEHSIAGYVALAKR
                     TVNIRDVYDENELRALNPSLRFLQEVDKRTGYRTKQMLVAPILDPGPNELIGVIQIIN
                     NKAGMAFSGLAEEGVGELAQTLAIAFKQRQKPQAAKSKYDYLIADAVLSAGEFELAAR
                     QARKKAIDVEQVLSEEFQVKIPAIGAALSKFFGVPYEPFKPDRVKPAELLRNLKREYV
                     EANQWVPIDDGREGLVVMCLDPERIRSSRIASNVFPKARIVYKVATQRDFKETVNQFY
                     GADTGADLGNVEDLLGAMSGDGSELLESTSFDEASAAADNELVKLVNKVILDAYQQGA
                     SDIHIEPYPGKAKTEIRFRKDGALGPYIEVPSSYRAAIAARIKIMCDLDISEKRRPQD
                     GKIKFKKFGPLDIELRVATIPTAGGVEDIVMRILNSGEPIPLEKMGFTEKNLSQLKSA
                     VSKPYGLFFVCGPTGSGKTTTLHSVLKFLNTPDTKIWTAEDPVEITQKGLRQVQVNKK
                     AGLDFSVVMKAFLRADPDIIMVGEMRDKETTGTGIEASLTGHLVLATLHTNSAPESVT
                     RLLDMGMDPFNFSDALLGILAQRLAKRLCSCKQPYTPEPAELTSFLREYCEELMNTAR
                     FKADPKGAMEAVYRDWVKAYGNDKGQLSLYKPVGCDKCGNSGFKGRCGLHELLIASDR
                     LKKAIQEHARVAEILALALEEGMRTLKQDGMEKCLTGITHIKEVRAVCIK"
     gene            5673..7292
                     /locus_tag="VFR57_04240"
     CDS             5673..7292
                     /locus_tag="VFR57_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU0258618.1"
                     /translation="MDTTPGLGLGAGGGRSEDLGRRLEQLNLIGASLSAERDINRLLE
                     LILAAAKSITGADGGTLYRVTEDNTLRFEIVRTSSLESYLGGTSGNPVPFYPIQLYRD
                     GKPNHSMVAAHAALTGKTVNIADAYAAEGFDFSGTRAFDSKTGYRSKSFLTVPMRNHE
                     HETIGVLQLINAREPETGAIVSFSDSDQRLAESLASQAAIALTNRMLINQLEQLFESF
                     INLINSAIDEKSPYTGGHCQRVPVLTMMLAEAVNETREGPLAAFHMTDKDRYELKIAG
                     LLHDCGKVTTPVHVVDKATKLETIYDRIHLIDTRFEVLKRDLELAFLKNHFTEKELHI
                     RLRELEDDRRFLRACNIGTERMSDEDVARVRRIAAYRWRDMTGQEANFLSDEEVKNLT
                     VRAGTLTEEERKIINHHIVATIKMLEALPWPKHLTRVPEYAGGHHERMDGKGYPRGLT
                     REQMSVQARCMGIADIFEALTARDRPYKKGKTLSESLQILGRMKLNNHIDPDLFDVFV
                     RRKVYWRYAEMFLDKEQIDEVDESRIPGYRV"
     gene            7328..9034
                     /locus_tag="VFR57_04245"
     CDS             7328..9034
                     /locus_tag="VFR57_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0258619.1"
                     /translation="MAYPPYLLAAYAITLPSVLIADFAAPSSDLIAALKLACIVAFAW
                     HAAPLLRNQPLGARSAAALAAAIGLLWSALGAMYLYKVVAMAPLDFSMVVLSIDDARR
                     TLVHVLGEYGVPLAVGALAATAAAASGAAWVLLSQLRARLARLPHSPRGAFACVGLLC
                     FFGASDLKYAAEELVLFPRVYSVDSAYTPALLSVPDYTAVPIRARESVFIVQLESVNS
                     LAVFEPAGEGTPFRPRLPQPGLETILKEGGGVLFPLFWANGTQTHRAWESILCAVSGN
                     LGPAMAADPGRLLRATCLPEHLGRGGYDTLFFYSYFNLEFFNLGEFARQAGFREVVYG
                     SKLMRADDPRHRWGFDDCAFYARAFDYLAGKGLDKRDRLFAYFEVGMHHAPFFNTTRH
                     PQAHPYPAPASFLQHYLNSNAEQDHCLLTFWTRFRELDRDDVHLFIVPDHSLWVHGVP
                     AYADVGFATWLAYVPPARRAREFKPRAVASPKPSQAQLYPTILELLEGASLSGSFAFA
                     LRGEPPPPGYADCHLLTEPGNRVVVRKNGERAEYRLRATSALGGASQSNFWDFQKNFA
                     CN"
     gene            9273..9743
                     /locus_tag="VFR57_04250"
     CDS             9273..9743
                     /locus_tag="VFR57_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU0258620.1"
                     /translation="MKSLQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKMSEVI
                     LGMSACRTSITEVYQSGAASAMVANGWGCEVGTTQQTKYVAGVTTSADGAVTATIQNI
                     SGSVDGSVVTLIPLASAGTAATYTGGSQTLYGWLCGGTGTNVNTKYLPGSCRGM"
     gene            9811..10980
                     /locus_tag="VFR57_04255"
     CDS             9811..10980
                     /locus_tag="VFR57_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0258621.1"
                     /translation="MRIAQKPISTDLTILMPCLSEAATVATCVAKARAFLQRSGIQGE
                     VLVADNGSTDGSQLAAERAGARVVHVERRGYGAALAGGIAAARGRYVIMGDADDSYDF
                     ARLEAFVEKLREGYPLVMGNRFRGGIEPGAMPRLHRYLGNPLLSFIGRLFFRARVGDF
                     HCGLRGFDREALLGLGLRTRGMEFASEMVVKAALAGWRIAEVPTRLHCDGRGRASHLR
                     SWRDGWRHLRFLLIFSPRWLFLYPGCTLLVAGIALTTALYFKPMQLLGAGLDIHSMLY
                     ASAGALLGMQLCLFSLFARVSAQAADLLPRRPALDRLLRLLTLERGLLAGAATALCGL
                     LWSASAFWQWQEEGFGALDPRVVMRDTIPAATLMVAGMEVMLASFLLSVLKWKDA"
     gene            10973..12067
                     /locus_tag="VFR57_04260"
     CDS             10973..12067
                     /locus_tag="VFR57_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0258622.1"
                     /translation="MPELSIVIPAYRERDNVVPMLSALERALPQCDWEAIFVVDDAFD
                     GTEDLVRERAQRDPRVRCLHRIGRRGLASACIEGMLASSAPYLAVIDADLQHDETLVP
                     QLLETAKREDADVVVASRFMEGGSTGDLAAERVRISRLASALSRLVCKGLTDPMSGFF
                     LVRRSFLERVVRRVYGRGFKVLLDLLAAARGGARIAELPYRMRRREHGESKLGARVIA
                     EFFMLILYHLTGRLLPARFFLFGAVGATGVAVHLGVLTAAFHVTGGNFLASQALATWA
                     AMTSNFFLNNAFTYADQRLRGAAVWRGLLSFYIACGIGALINLAVAEWLYLQSVAYWI
                     AGVAGALIAAMWNFFTTASVTWGRPEVRRR"
     gene            12067..>13484
                     /locus_tag="VFR57_04265"
     CDS             12067..>13484
                     /locus_tag="VFR57_04265"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU0258623.1"
                     /translation="MPAGRELRPTRADWAQAAGVAAALLGLYALTAPRTVALEDDGLF
                     VLSSYFLGIEHPPGFPLFTLIGHLFTYLPFGSVAYRVHLASAFFGALSCGLLWLCARS
                     LVPGRLPAYLAALGLGFSPAFWSQALIAEVYTLNSFFFLLLAHLGLRACPPGDEAPNP
                     RLLPWLALLFGLSLSNHWPLMGLAAPAFAVLLWPLRREVLARLGLISWLVLAGLLPYA
                     WLVYRSWMAVPISYYGPLETLTEIWFFLSRAGYAEVDVQPGAGWLDRLKFLQFVILEV
                     GRQFAVAGALLGLVGLAVQRRMLGTRLAAFLVVAFCMPTVVLLLLLGFSYNSVSRHVF
                     HVYPLPAYAVVALWAAIGFAWLRKRLALRPAAATAACGGVLAAILAVGGRYNYGTDHE
                     WGAHYAQVLARTLPRDAVLFVKGEFDIGPLGYLHMVEGLRPDITLYQYKGLALGNRLF
                     HPLRTTEATASRVVREFIEAQQS"
BASE COUNT         2336 a         4443 c         4382 g         2323 t
ORIGIN      
        1 cctttttcag gtcgccgtcg aacccgatga gctgggtgcc cgcgatagca tcgatatgca
       61 acaatcgggt cggtactttc ggcgagcctt ggaatgcgct gctcaggctc cagtgctggg
      121 cctcggcctt gaggaccgtg atgcgcgaga atgaattcca gcgctcgtac tcgacatcga
      181 acccgctaaa tgcgctctgg attttgaacg gagagattcc gtggcggtcg aggtagaggc
      241 ccgaccatag cgtcgtgccg gcgagcgcag cggtcaccgc cgcggcaagc gccctccctt
      301 tgccgcgcag caggaagatt ccggcaagcg cggccaacgt tgcagcggcg agaacggcac
      361 ctacgccgtc aagccagcgc agggcggcga tgacgccgag gcaccctacc gccgcgcccg
      421 ccaggtctgc ggcataaagc tttccgacac tctgggggaa tcgcgtgagt gccaggcaaa
      481 cgaatatgcc cgcgaagaca aacggcacgg cagcgcagac gaacgtgacg atgactggcc
      541 aaatgtactc gcccggcttc ggcacataga gcgagatatg caaaaaggtc acagcaacca
      601 tggaaatgga gcaaagaacg gcgcaccgcc ccatggcgct tgccaacctt tctgccggcc
      661 accatgccgg acgcaggaac actgcgagcg caccgatcgt catgccgaac atggcgatcg
      721 aaatggccat gaaggcgaag tggtaccaca tcgtgagaga aaacacgcgc gtcagcaaga
      781 cctgaaacat gatcgtgact agcgcgacca ggaacagcgc cgtgaaaagc ccgcgcggtg
      841 aaaatcgatc catttatcct tcttgttatt gagcagtagc aactggccat cctagcagcc
      901 gatctcccga cgtcgaggaa aagttgccgg taattcgaag gctgcaggcg agctagctta
      961 gagaaggtag tcaaccccgt ggcgcgcgat ccccatcgct acgacatgag ccgaagagcg
     1021 ctgggatcat gcgtgcgccg cgcccacgcc gccatggggg acgcggtcaa ggtggcctca
     1081 cgcccggaag ggcgcacaaa accttacggt gacgggatcc ggaggcaggc gacgcgcaag
     1141 cgggtcaaag atgtggtatt cagggaaatc gagcgcatcc gggtcaagag gaaagagggg
     1201 gctgtcacta tcgcgcccaa cgctggaacg aagccgagat gctcctgcgc gagctgcatc
     1261 gcatgggccc cgcgcgagga ctgtgcgccg tctacctcgc ccggctcgag gactcgcgcc
     1321 tgcacccgcc gccgccggaa tggaacggcg tgacggcatt cgacgaaaag tagaggggtt
     1381 cggcgcatgc gtttgcgcgt gctcggctgc tcggggggga tcggagggcg ccacctgcgc
     1441 acaacgtcgt ttctcctcga tagcgacatc ctgatcgacg ccggcaccgg ggtcggcgac
     1501 ctgacgctcg cggagctctc gcagattgac cacatcttca ttacgcattc gcacctcgac
     1561 cacgtcacct caatcccgtt cctggtcgat accgtcggcg gcatgcggtc gcggccgctc
     1621 aacgtgtacg cgacgcgggc gaccatcgag atcctgaaga accatctctt caactgggcg
     1681 atctggccgg acttcaccga gataccgagc ctggaggcgc ccttcatgcg ctacctcgag
     1741 atcgagttgg ggaccgcggt aacgctcggc ggacgcacca tcacggccat tccggcgcgc
     1801 cataccgtcc cggcggtggg ttaccacctg gatagcggcg cggcgagcct ggtattcacc
     1861 ggcgacacgg ggccgaacga cacgctttgg cgcatcgtga accgcatcgc caaccttaag
     1921 ttcctcatca tcgagactgc gttctccaac aaggaacgcc agctcgccga gatctcgcgc
     1981 catctttgtc cggcaacgct cgccgaggag ctcgccaagc tcgagcgcga tcccgagatc
     2041 tacatcaccc atctcaagcc cggcgagatc gagccgacca tgcttgaaat cgaggagtgc
     2101 gcgggagact tcaagccgcg catgctgcag aacaacctgg tgctggagtt ctagcgcgca
     2161 cgatgaacgg ctcgctgcgg cccacgcgcg accagcttgc catgctggtt ccgctcgccg
     2221 ggctgtcgcg cgagcgcctc gcggagctcg ccgaagccac gttgatcgag cgcgcggcgc
     2281 gcggcgccga ccttctcgcc gggcgtgcgc ggccggggcc gtcgctgttc ctgctcgcgg
     2341 gcgaggttct actcggcttc gaggcggggg gcacgctggt cgtggtcggc ggcaccgagg
     2401 agacgcgcca ggcgctcaac cggcgcaagg aacgcgtggc gcgcgcgcgc gccatcaccg
     2461 atgtcgacct gctcgtggtc gaggacgagt tgctcgacgt ccttgccacc tgggaccagg
     2521 tggcggctgc gcgcgcgccc gacgccgtgg caagcggcgc gttttcgctc tcctccctgc
     2581 gcgccggcgt gttcgcgcag ctgccgccgg cccacatcga tgagctgctg cggcgcttcg
     2641 agcgcgtgaa cgcagcgcgc ggcgaagtgg tgatccgcga aggcgacgaa ggcgactact
     2701 actacgtcat ccaatcgggc cgcttccagg tcgaacgcct ggtcggcggc gcggacgtcg
     2761 tgctcgccga gctcaaggtg ggcgatgcct tcggcgaaga agcgctcgtc gccgaggcga
     2821 agcgaaacgc cacggtcacc gcgctgaccg acggtcagct gctccgcctg aaccgtcccg
     2881 atttcatcga gctgttgcgc gagccgttgc ttcggcgtat cggcttcgag gaagcgtccg
     2941 caaaagtgcg ccgcggcgcg ctgtggctcg acgtgcgcta cccctccgag taccagtacg
     3001 acaagctgcc gggcgcgatc aacgtgccgc tcgcggaagt gcggaatatg ttcgcggtgc
     3061 tcgatcgcac taaggagtac attgcgtact gccagagcgg ccggcgcagc gctgcggccg
     3121 cgttcctttt cgcacaaaga ggcttcaagg tctggctgct ggagcacggc ttgaaaggcg
     3181 tggagaagcc ggaaagccgt tgaaaatcag gtatcctagc gagaagaagt cgcagggagg
     3241 gtagcgaatg agcgcagtcc tgagtccgca cgcatcggcg agcagcgacg tcggcgcgcg
     3301 cctcgcgttc cagaagcagc tgcaggcggt cacgaacaag atccacgcga ccagcaacat
     3361 cgacgagatc atgctcgagg tgagcgccga cgtctgcgtc ctgttcaacg ccgatcgcct
     3421 cacgatctac tcggtgggcg aggacaggca gtctatcgtc tccaaggtca agaccggcct
     3481 caattcgttc aaggacctca agctgccgat cgccgagcac tcgatcgcgg gctatgtcgc
     3541 gctggcgaag aggacggtga acatcaggga cgtgtatgac gagaatgagc tgcgcgcgct
     3601 gaaccctagc ctgcgattcc tgcaggaagt cgacaagcgc accggctacc gcaccaagca
     3661 gatgctggtc gcgccgatcc tggatcccgg accgaacgag ctgatcggcg tcatccagat
     3721 catcaacaac aaggccggca tggccttcag cgggctggcc gaagaagggg taggggagct
     3781 tgcgcagact cttgccatcg cgttcaagca gcgccagaag ccgcaggccg cgaagagcaa
     3841 atacgactat ttgattgccg acgcggtgct gtcggcgggc gagttcgagc tcgctgcgcg
     3901 ccaggcgcgc aagaaggcga tcgacgtcga gcaggtgctg agcgaggagt tccaggtcaa
     3961 gatcccggcg atcggcgccg cgctctccaa gttcttcggc gtgccctacg agccgttcaa
     4021 gcccgaccgg gtgaagcccg ccgagctgct gaggaacctg aagcgcgagt atgtcgaggc
     4081 gaaccagtgg gtgccgatcg acgacggcag ggaaggactg gtggtgatgt gtctcgatcc
     4141 cgagcgcatc cgctcttcgc gcattgcatc caacgtgttc ccaaaggcgc gtatcgtcta
     4201 taaggtcgcc acgcaaaggg acttcaagga gacggtgaac cagttctatg gcgccgacac
     4261 cggcgccgac ctcggcaacg tcgaggacct gctcggcgcg atgagcgggg acggcagcga
     4321 gctgctcgaa agcacgtcct tcgacgaggc ctcggccgcg gccgacaacg agctggtcaa
     4381 gctagtgaac aaggtgatcc tggacgcgta ccagcagggg gcctcggaca tccacatcga
     4441 gccctacccg gggaaggcca agaccgagat ccgcttccgc aaggacggcg ccctcgggcc
     4501 gtacatcgag gtcccgtcga gctatcgcgc tgcgatcgca gctcgcatca agatcatgtg
     4561 cgaccttgat atctcggaga agagaaggcc gcaggacggc aagatcaagt tcaagaagtt
     4621 cggcccgctc gacatcgagc tgcgcgtggc gacgattccg accgcgggcg gcgtcgagga
     4681 catcgtgatg cgcattctca actctggtga gccgattccg ctcgagaaga tgggtttcac
     4741 cgagaaaaac ctctcgcagc taaagagcgc agtgtcgaag ccctacggcc tgttcttcgt
     4801 gtgcgggccc accggctcgg gaaaaaccac cacgctgcac tcggtgctca agtttctgaa
     4861 cacgcccgac accaagatct ggaccgcgga agacccggtg gagatcacgc agaaggggct
     4921 gcgtcaggtg caggtgaaca agaaggcggg cctggacttc tccgtggtga tgaaggcgtt
     4981 cctgcgcgcc gacccggaca tcatcatggt gggcgaaatg cgcgacaagg agaccaccgg
     5041 caccggcatc gaagcgtcgc ttactggcca cctggtgctc gcgacgctcc acaccaactc
     5101 cgcccccgag tccgtcacgc gcctgctcga tatgggcatg gacccattca acttttccga
     5161 cgcgctgctc ggcatcctgg cccagcgcct ggcaaagcgc ctgtgcagct gtaagcagcc
     5221 ctacacgccc gagcccgccg agctcacctc gttcctgcgc gagtactgcg aggaattgat
     5281 gaacactgcc cggttcaagg ccgacccgaa gggggcgatg gaggcggtgt accgcgactg
     5341 ggtcaaggcc tatggcaacg acaagggtca gctctcgctc tacaagccgg tcggctgcga
     5401 caagtgcggc aactcgggct tcaagggccg ctgcggcctg cacgagctgc tgatcgcgag
     5461 cgatcggctc aagaaggcga tccaggagca cgcccgcgtt gccgagatcc tcgcgctcgc
     5521 cctagaggaa ggcatgcgta cgctgaagca ggacggcatg gagaagtgcc tgacgggcat
     5581 cacccacatc aaggaagtgc gcgccgtttg tatcaagtaa aacgacatgc agaacggccc
     5641 gtaggacggc ccgcagccgg cccgtgaatg tcatcgatac cacgccgggc ctggggcttg
     5701 gggccggggg cggacgttcc gaggacctcg gccggcgcct cgagcagctg aacctcatcg
     5761 gtgcgtcgct ttcggccgag cgcgacatca atcgcctgct cgagctgatt ctcgcggcgg
     5821 cgaagtcgat caccggggcc gacggcggca ccctctaccg cgtcaccgaa gacaacactt
     5881 tgcgcttcga gatcgtgcgc acgagctccc tcgagtccta cctcggtggc acgagcggca
     5941 acccggtgcc cttctacccg atccagctct accgcgacgg caagccgaac cacagcatgg
     6001 tcgcggcgca cgccgcactt accggcaaga cggtgaatat cgctgacgcc tacgccgccg
     6061 aaggcttcga cttttcgggc acgcgtgcgt tcgactcgaa gaccggctac cgctccaagt
     6121 cgtttctcac cgtgccgatg cgcaaccacg agcacgagac catcggagtg ctgcagctca
     6181 tcaacgcgcg ggagccggaa acaggcgcga ttgtgtcgtt ctcggactcc gaccagcgcc
     6241 tcgccgagtc gctcgcgtcg caggcggcga tcgcgcttac caaccgcatg ctgatcaatc
     6301 agctcgagca gctgttcgag tcgttcatca atctcatcaa ctcggcgatc gacgagaagt
     6361 caccttatac cgggggacac tgccagcgcg tgccggtgct gacgatgatg ctcgccgagg
     6421 cggtgaacga aacacgcgag ggcccgctcg ccgccttcca tatgaccgac aaggaccgct
     6481 acgagttgaa gattgcgggc ctgctgcacg actgcggcaa ggtcaccacg ccggtgcacg
     6541 tggtcgacaa ggcgaccaag ctcgagacca tctacgaccg catccacctc atcgacacgc
     6601 gcttcgaggt tctgaagaga gatctcgagc ttgctttcct taagaatcac ttcacggaaa
     6661 aggagctgca cattcgcctg cgcgagctgg aggatgaccg ccgcttcctg cgcgcgtgca
     6721 acatcggcac ggagcgcatg tccgacgaag atgtcgcgcg agtccgccgc atcgccgcgt
     6781 accgctggcg cgacatgacg gggcaggagg ccaacttcct gtccgacgaa gaggtgaaga
     6841 acctcaccgt tcgcgccggc acgctgaccg aggaggagcg caagatcatc aaccaccaca
     6901 tcgtggccac gatcaagatg ctcgaagccc tgccgtggcc caagcatctc acgcgggtgc
     6961 ccgaatacgc cggcggccac cacgagcgca tggacggcaa gggctacccg cgcgggctga
     7021 cgcgcgagca gatgtcggtg caggcgcgct gcatggggat cgccgatatc ttcgaggcgc
     7081 tcaccgcccg cgaccggccg tacaagaaag gcaaaaccct gtcggagtcg ctccagatcc
     7141 tgggacgcat gaagctcaac aaccacatcg accccgatct gttcgacgtc ttcgtgcgca
     7201 ggaaggtgta ctggcgctac gccgagatgt tcctcgacaa ggagcagatc gacgaggtgg
     7261 acgagtccag gatcccgggt taccgcgtct gagcggccaa gtcacctggc gcccgcaagc
     7321 cgcgcgcgtg gcctatcctc cgtacctgct cgcggcctac gcgatcacgc tccccagcgt
     7381 cctgatcgcc gacttcgccg caccctcgag cgacctgatc gcggcgctca agctcgcctg
     7441 catcgtcgcc ttcgcgtggc acgcggcccc gctgctgcgc aaccagccgc tgggcgcaag
     7501 aagcgcggcc gcgcttgccg cggcgatcgg gctgctgtgg agcgcgctgg gcgcaatgta
     7561 cctctacaag gtcgtggcca tggcgccgct cgatttctcc atggtggtgc tgtcgatcga
     7621 cgacgcgcgc cgcacgctgg tccacgtgct cggcgaatac ggcgtgccgc tcgctgtggg
     7681 ggcgctcgcc gcaactgcgg ccgcggcgag cggcgcggca tgggtgttgc tcagccagct
     7741 ccgcgcgcgc ctcgcgcgcc tgccgcacag cccgcgcggc gccttcgcct gcgtggggct
     7801 actgtgcttt ttcggcgcga gcgacctcaa gtacgcggcc gaggagcttg tcttgtttcc
     7861 gcgggtctac tccgtcgata gcgcctacac tccggcgctg ctcagcgttc cagactacac
     7921 cgcggtcccg atccgcgccc gggaaagcgt tttcatcgtg cagctcgagt cggtgaattc
     7981 gctggcggtg ttcgaaccgg ccggggaggg gacgccattc aggccgcggc tgccgcagcc
     8041 cgggctggag accatcttga aggaaggtgg cggcgttctg ttcccgctgt tctgggcgaa
     8101 cggcacgcag acccaccgcg cttgggaaag cattctgtgc gcggtgagcg gcaatctcgg
     8161 gccggccatg gcggccgatc cgggccggct gctgcgcgcc acctgccttc ccgagcacct
     8221 aggccgcggc ggctacgaca cgctgttctt ctattcgtac ttcaacctgg aattcttcaa
     8281 cctcggtgag ttcgcgcgtc aagccggttt ccgcgaggtg gtctacgggt cgaagctgat
     8341 gcgtgcggac gacccccgcc accggtgggg gttcgacgac tgcgcgttct acgcccgcgc
     8401 gttcgactat ctcgccggta aaggcctgga caagcgagac cggctcttcg cctacttcga
     8461 ggtcgggatg caccacgcgc cgttctttaa cacgacgcgg caccctcaag cgcatccgta
     8521 tccggcgccg gcgagctttc tccagcatta cctgaactcg aatgccgagc aggatcactg
     8581 cctgctcacg ttctggacgc gcttccgcga gctcgaccgc gacgacgtgc atctattcat
     8641 cgtcccggat cacagcctct gggtgcacgg cgtgccggcg tatgcggatg tgggcttcgc
     8701 cacctggctt gcctacgttc ccccggcgcg acgcgcccgc gaattcaagc cgcgcgcggt
     8761 tgcctcgccg aagccgagcc aggcgcagct ctatcccacg atcctcgagc tcctggaagg
     8821 cgcgtcgctt tcgggctcgt tcgcctttgc gctgcgcggc gagccgccgc cgcccggcta
     8881 cgccgactgc catctgctga ccgagccggg caaccgagtg gtggtgcgga agaacggcga
     8941 gcgcgccgag taccgcctgc gcgcgacgtc agcgctcggg ggtgcgtccc agtccaattt
     9001 ctgggacttt cagaagaact tcgcatgcaa ttaggcacgt cggctaccag ccgcaatcta
     9061 gtggaaataa gtcgcagccg ttcggactag gttgctgatg cgtgaccaaa attgcccgtc
     9121 actgacaata aggggcagag cggagaggca aacccgtgat ttagtttgct tgcttgtgcc
     9181 tcgcaggaca tggcacgagt attgcgagtt atcggcgggt cggtacaggg ccgaccggaa
     9241 agaattctca ctaaattgga agggagtaaa gcatgaagag tctgcaaaag ggcttcaccc
     9301 tgatcgaact gatgatcgtg gtggcgatca tcggcattct ggcggcggtc gcgctgccgg
     9361 cgtatcagga ctacaccatc cgcgcgaaga tgtcggaggt gatactgggc atgagcgctt
     9421 gccgcacctc gatcaccgag gtgtaccagt cgggcgccgc ttcggccatg gtggcaaacg
     9481 gctggggctg cgaagtcggc acgacccagc agacgaagta cgttgcgggc gtcaccacga
     9541 gcgcggacgg cgcagtgact gcgacgatcc agaacatcag cggctcggtc gacggctcgg
     9601 tagtcacgct catcccgctg gcttctgcgg ggacggcggc cacctacact ggcggctcgc
     9661 agaccctcta cggctggctc tgcggcggca ccggcacgaa cgtcaatacg aaatacctgc
     9721 caggctcctg ccgcggcatg taacaagcgt ccagcatcag gaaaggtacc ccgaaccggg
     9781 gtaccttttt ttttgggggg atggcttttg gtgaggatag cgcagaagcc gatatcaacg
     9841 gacctgacga ttctcatgcc gtgcctgagc gaggcggcaa cggtcgcgac ctgcgtcgcc
     9901 aaggcgcgag cttttctcca gcgttccgga atccagggcg aggtgctggt ggcggacaac
     9961 ggctcaaccg acggctcgca gctcgccgcc gagcgcgccg gggcgcgcgt agttcatgtt
    10021 gaacgacgcg gctacggcgc cgcgctcgcc gggggtatcg ccgcggcacg cggccgctac
    10081 gtcatcatgg gcgacgccga cgacagctac gacttcgcgc gcctcgaggc cttcgtcgag
    10141 aagctgcgcg agggctaccc cctcgtcatg ggcaaccggt tccgcggcgg catagagccg
    10201 ggcgcgatgc cgcgcctgca ccgctatctg gggaatccgc tgctcagctt catcggtcgg
    10261 cttttcttcc gggcgcgcgt cggggacttc cattgcgggc tgcgcggctt cgatcgcgag
    10321 gcgctgctcg gcctcgggct gcgcacccgg ggcatggagt tcgcgagcga gatggtggtg
    10381 aaagcggcgc tcgcgggttg gcggatcgcc gaggtgccga cccggctgca ttgcgacggc
    10441 cgcggcaggg cttcgcactt gcgcagctgg cgcgacggct ggcggcacct gcgctttctg
    10501 ctcatcttca gcccgcgctg gcttttcctt tatccgggct gcacgctcct cgtagccggg
    10561 atcgcgctga ccaccgcgct ctacttcaaa ccgatgcagc tgctcggcgc cgggctcgat
    10621 atccacagca tgctctatgc gtccgcgggc gcgcttctcg gcatgcagct ctgcctgttc
    10681 tccctgttcg cgcgcgtctc ggcgcaagct gcagatcttc ttccacgccg gccagccctg
    10741 gatcgcctgc ttcgcctgct aacactcgag cggggactgc tggccggggc ggcgacagcc
    10801 ctttgcgggc tcttgtggag cgcgtctgcc ttctggcagt ggcaggagga ggggtttggc
    10861 gcgctggatc ctcgcgtcgt catgcgcgac acgattcccg cggcaaccct catggtcgcc
    10921 ggcatggagg tcatgctcgc atcgttcctg ctcagcgtac tcaagtggaa agatgcctga
    10981 gctctcgatc gtcattcccg cataccgcga gcgcgacaat gtcgtgccga tgctgtcggc
    11041 gctggagcgg gcgctgccgc aatgcgattg ggaggccatc ttcgtcgtcg acgacgcgtt
    11101 cgacggcacg gaggacctgg tgcgcgagcg ggcgcagcgc gacccgcgcg tacgctgcct
    11161 gcaccgcatc ggccggcgcg ggctcgcctc ggcgtgcatc gaaggcatgc tcgcgagctc
    11221 ggcgccttac ctcgcggtga tcgacgccga cctgcagcac gacgagacac tggtgccgca
    11281 gctgcttgag accgcgaagc gcgaggatgc cgacgtggtg gtcgcgagcc gcttcatgga
    11341 gggcggcagc accggcgacc tggcggccga gcgcgtgcgc atcagccgcc tcgcgagcgc
    11401 gctcagccgg ctggtttgca agggactcac cgacccgatg agcggctttt tcctcgtgcg
    11461 gcgcagcttc ctcgagcgtg tcgtgcggcg cgtgtacgga cgcggcttca aggtcctgct
    11521 cgatctgctc gccgctgcgc gaggcggtgc gcggatcgcc gagctgcctt accgcatgcg
    11581 cagacgcgag cacggcgaga gcaagctcgg ggcgcgcgtg atcgccgagt tcttcatgct
    11641 cattctctat caccttaccg gccggctgct accggcgcgg ttcttcctgt tcggcgccgt
    11701 cggcgccacg ggcgtggccg tgcatctcgg cgtgctgaca gccgcgtttc acgtcacggg
    11761 cggcaatttc ctcgcgagcc aagccctcgc cacgtgggca gcgatgacca gcaacttctt
    11821 cctcaacaac gcgttcacct acgccgacca gcggctgcga ggcgccgccg tctggcgcgg
    11881 ccttctcagc ttctatattg cctgcggcat cggcgcgctt atcaatctcg ccgtggccga
    11941 gtggctctac cttcagtccg tggcgtactg gatagccggc gtggcggggg cgctaatcgc
    12001 cgcgatgtgg aacttcttca caaccgcgtc ggtgacctgg ggccggcccg aagtcaggag
    12061 gcgctgatgc cggcgggacg ggagcttcgc ccgactcgtg ccgactgggc acaggccgcc
    12121 ggcgtggcag cggcgctcct cgggctctac gcgctgaccg cgccacgcac cgtcgccctg
    12181 gaggacgacg ggctgttcgt cctttcgagc tatttcctcg gcatagagca tccgcccggc
    12241 ttcccgctct tcacgctgat cggccacctg ttcacgtacc tgcctttcgg ctcggtcgcc
    12301 tatcgcgtcc acctcgcaag cgccttcttc ggcgcgctaa gttgcggcct tctgtggctg
    12361 tgtgctcgct cgctcgtgcc cggccgcctg cccgcatacc tggcggcgct cgggctcgga
    12421 ttttcgccgg cattctggtc gcaggcgttg atcgccgagg tctacacact caacagcttc
    12481 ttcttcctcc ttctcgctca cctcggcctg cgcgcctgtc cgccgggaga tgaggcgccg
    12541 aacccgcggc tgctgccctg gctcgcgctt ctcttcgggt tgagcctcag caaccactgg
    12601 ccgctcatgg gcctggctgc gccggctttc gccgtactcc tctggccgct gcgccgggag
    12661 gtgcttgcgc ggctgggcct gatctcctgg cttgtgctcg ccggcctgtt gccctatgcc
    12721 tggctcgtgt accgctcctg gatggccgtg cccatcagct actacgggcc gctcgagacg
    12781 cttaccgaga tctggttttt cctcagccgc gccggctacg ccgaagtgga cgttcagccc
    12841 ggtgccggct ggctcgaccg gctgaagttc ctgcagttcg tcatcctcga agtcggccgg
    12901 cagttcgcgg tcgcgggcgc gctgctcggc cttgtcggcc tagcggtgca gaggcgaatg
    12961 ctcggcacgc gacttgccgc cttcctcgtt gtcgcctttt gcatgccgac ggtggtgcta
    13021 ttgctgctac tcggcttcag ctacaactcg gtgagcaggc acgttttcca cgtctatccg
    13081 ctgccggcct acgccgtggt cgcgctgtgg gccgcgatcg gcttcgcctg gctgcggaaa
    13141 cggctcgctt tgcgccccgc ggccgcgacg gctgcctgcg gcggcgtgct tgcggcgatt
    13201 ctggccgtcg gtggccgcta taactacggc acggatcacg agtggggcgc gcactatgcg
    13261 caggtgctcg cccgcacgct gccgcgcgat gccgtgctct tcgtgaaggg agagttcgat
    13321 atcggcccgc tcggctacct ccacatggtc gaaggcctgc ggcccgacat cacgctctac
    13381 cagtacaagg ggctggcgct cggcaaccgg ctgtttcatc ccctgcgcac gaccgaggcg
    13441 acggcgagcc gcgtggtgcg cgagttcatc gaggcgcagc aatc
//
LOCUS       DASTPD010000121         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_879505, whole genome shotgun sequence.
ACCESSION   DASTPD010000121 DASTPD010000000
VERSION     DASTPD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879505"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..537
                     /locus_tag="VFR57_04270"
     CDS             118..537
                     /locus_tag="VFR57_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0258624.1"
                     /translation="MLALSGAIALDTRSLSFWDDTTPGPAFLPVWLAIAGAVLFVLRL
                     AEARRAGGGPSVQRPDRAAIVRVATIFAGLVAVPLLAPVVGLVPAVALLVAFVLLFVL
                     RQAFWPSVATVGITAGLIYAIFVGWLGVPLPRGPLGL"
     gene            541..2040
                     /locus_tag="VFR57_04275"
     CDS             541..2040
                     /locus_tag="VFR57_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0258625.1"
                     /translation="MEPLQLLGQGFATCFTPLNLSFIFIGVLIGQIIGALPGIGPAAG
                     MALLLPLTYGVPPVTAIMMLAGIMYGGQYGGTLTSVLVNVPGEAASVMTTIDGHQMAR
                     QGRAGAALSIAAIGSFFAGIGAVVVVVFVTPPLAEFALKFNAPEYFLLALLGITAAAS
                     LGADSPTKALLAALLGLMTAMVGVDPIQGTPRLVFGQQSLIEGIDFLPVAIGIFGIAE
                     VLASLERSEHVAPMRTRLRDMWLTAQDWAESRLAILRGALIGFFIGVMPGAGPTIASM
                     LAYIAEKRLSRRPERFGRGAIDGVAAPESANNAASHAACVPMLALGIPGSASTAVLLA
                     ALILHGIRPGPGLMTEQSTLVWALIASMFIGNVVLLVLNLPLAPLFAALLRVRYAYLA
                     PGILAISLVGAYANTLGFSNVWIALVFGVIGWFMIKLDFPRAPLVLALVLAPLLESSL
                     RQSLLLSFGSLKIFVERPLAAALLACVVLSLALPLVGMLRRSARLKNVG"
     gene            complement(2033..2107)
                     /locus_tag="VFR57_04280"
     tRNA            complement(2033..2107)
                     /locus_tag="VFR57_04280"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2073..2075),aa:His,seq:gtg)
     gene            complement(2142..2218)
                     /locus_tag="VFR57_04285"
     tRNA            complement(2142..2218)
                     /locus_tag="VFR57_04285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Arg,seq:tct)
     gene            complement(2225..2301)
                     /locus_tag="VFR57_04290"
     tRNA            complement(2225..2301)
                     /locus_tag="VFR57_04290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2265..2267),aa:Pro,seq:tgg)
     gene            2407..2832
                     /gene="dksA"
                     /locus_tag="VFR57_04295"
     CDS             2407..2832
                     /gene="dksA"
                     /locus_tag="VFR57_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEU0258626.1"
                     /translation="MPVELTRKSFTPYAAKKGEEYMNARQRSHFRKILESLKAELSEE
                     IDRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIARIDANEYG
                     YCDSCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            complement(2842..4950)
                     /gene="pnp"
                     /locus_tag="VFR57_04300"
     CDS             complement(2842..4950)
                     /gene="pnp"
                     /locus_tag="VFR57_04300"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU0258627.1"
                     /translation="MNPIKKSFSYGAHQVTFETGEIARQAHGSVICSMDDTVVLATVV
                     AAKEARAGQDWFPLTVDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFLNEVQVVATVLSLNPEIDSDIPAMLAVSAALEISGIPFNGPIGACRVGYVGGDY
                     VLNPTASQLKESKLNLVVAGTEQGVMMVESEALELPEDTMLGAVMYGHQQMQSAIQAI
                     HELAEQAGKPAWDWQLPAKNEALIGRVAELVEQDFRAAFALKQKQARNTRLAEIRKTA
                     LDKLLAENPQGDVNVVKETFFNLESKIVRSQILDGEPRIDGRDTRTVRPINVRVGVLP
                     RTHGSALFTRGETQALVTVTLGTGRDEQIIDALSGEYRERFMLHYNMPPYATGETGRV
                     GPPKRREIGHGRLAKRALVGLLPTPEEFGYSIRVVSEITESNGSSSMASVCGGSLALM
                     DAGVPVKAHVAGIAMGLIKDGNRFAVLSDILGDEDHLGDMDFKVAGTDKGITALQMDL
                     KIESINKGIMKVALDQAREGRLHILEIMKQAIEGSRSELSSYAPRILKIKINPDKIRD
                     VIGKGGVVIRGIQEETGTTIEIEDDGTVSIACVSAEGGEAAKKRIQELTADVEVGKVY
                     EGTVLRLLDFGAIVQLLPGRDGLLHISQISHERVNAVSDHLKEGQQVKVKVLEADDKG
                     RVRLSMKAVKEQEQPANQSG"
     gene            complement(5020..>5160)
                     /locus_tag="VFR57_04305"
     CDS             complement(5020..>5160)
                     /locus_tag="VFR57_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0258628.1"
                     /translation="KTHVKDFHSRRGLLKLVSQRRKLLDYLKRKDADKYRGLIERLSL
                     RK"
BASE COUNT          796 a         1715 c         1667 g          982 t
ORIGIN      
        1 aggccggcga gtacttcaag cgcgaagagg cgaagttcag gtcgctgatg gaagacgtcg
       61 gtctggcgaa gaagtagccg gtcgatgcgg ctgagcgacg tcgtcccggc gttgctcatg
      121 ctcgcgctgt cgggtgcgat cgcgctcgac acgcgcagcc tgagcttctg ggacgacacc
      181 acgcccgggc cggcattcct gccggtgtgg ctcgccatcg cgggcgcggt gctcttcgtt
      241 ctgcgcctgg cggaggcgcg ccgcgctggc ggcggcccgt cggtgcaaag gcccgatcgt
      301 gcggccatcg tgcgggtggc gacgattttt gcaggcctgg tcgcagtgcc cctgctcgcg
      361 cccgtcgtcg gcctcgtgcc tgccgttgcg ctgctcgtcg cgtttgttct tctgttcgtc
      421 ctgcgccagg cgttctggcc gagcgtggcg acggtaggga ttactgcggg cctgatctac
      481 gcgattttcg tcggctggct cggcgtgcca cttccccggg ggcctctcgg actctaggga
      541 atggaaccgc tacagctcct cgggcagggg ttcgcgacgt gcttcacgcc gctcaacctg
      601 tctttcatct tcatcggcgt cctgatcgga cagatcattg gcgccctgcc cggtatcggc
      661 cccgccgcag gcatggcgct gcttttgccg ctcacctacg gcgttccgcc ggtgaccgcc
      721 atcatgatgc tggccggcat catgtacggt ggtcagtacg gcggcacgct cacctcggtg
      781 ctcgtcaacg tgccgggcga agcggcgagc gtaatgacga ccatcgacgg gcaccagatg
      841 gcgcgccagg ggcgagccgg cgcggccctg agcatcgcgg cgatcggctc gtttttcgcc
      901 ggcatcggcg cggtcgtcgt tgtggtattc gtcactccgc cgctcgctga gtttgccctc
      961 aagttcaacg cgcccgagta ttttctgctg gcgctcctcg gcatcaccgc ggccgcgagc
     1021 cttggcgccg actcgccgac caaggcgctg ctcgcggcgt tgctcgggct gatgaccgcc
     1081 atggtcggcg tcgacccgat ccagggcacg ccgcgcctcg tgttcgggca gcaaagcctg
     1141 atcgagggaa tcgacttcct gccggtggcg atcggcatct tcggcatcgc tgaggtgctc
     1201 gcgtcgctcg agcgctcgga gcacgtcgcg ccgatgcgca cgcggctgcg cgacatgtgg
     1261 ctcaccgcac aggattgggc cgaaagccgc ctcgcaattc tgcgcggcgc gctgatcggc
     1321 ttcttcatcg gcgtaatgcc cggcgccgga ccgacgatcg cctcaatgct cgcctacatc
     1381 gccgagaagc gcctcagcag gcggccggag cgcttcgggc gtggcgcgat cgacggcgtg
     1441 gccgcgcccg aatcggcgaa caacgccgcc tcgcacgcgg cctgcgtgcc gatgctcgcg
     1501 ctcggcattc ccggctcggc gagcaccgcg gtgctgctcg ccgcgctgat ccttcacggc
     1561 atccggcccg gcccggggct gatgacggag caatcgacgc tggtctgggc gctcattgcc
     1621 agcatgttta tcggcaatgt ggtgctgctt gttctcaacc tgccgctcgc gccgcttttc
     1681 gcggcgctgc tgcgggtgcg ctatgcctac ctcgcgccgg gcatcctggc gatctcgctg
     1741 gtaggcgcgt atgccaacac gctcggcttc agcaatgtct ggatcgccct cgttttcggc
     1801 gtcatcggct ggttcatgat caagctcgat ttcccgcgcg cgccgctggt gctcgcgctc
     1861 gtgctcgcgc cgctgctcga gagctcgctg cgacagtcgc tcttgctttc cttcggcagc
     1921 ctgaagatct tcgtcgagcg gccgctcgcc gccgcgctgc tcgcctgcgt cgtcctgtct
     1981 ctcgcgcttc cgctggtcgg catgctgcgg cgctccgcgc gcctgaagaa tgtggggtga
     2041 ccgatgggac tcgaacccac aacaaccgga atcacaatcc gggactctac ctttgagcta
     2101 cggtcaccgc agttgcaaga acttgcaaaa agctccggcc ctggcctgcc cggcgggact
     2161 cgaacccaca acccccagct tagaaggctg gtgctctatc cggttgagct acgggcaggt
     2221 cccgtggtcg gggtgagagg atttgaacct ccgacatctt gctcccaaag caagcgcgct
     2281 accaggctgc gccacacccc gaaaaaggcg tcaaatgata ctcttagcgc atacccagag
     2341 caatcgaagt cgcttgcgac aggggggctt tagtgctaaa aagcgctttc gctttttgag
     2401 ccgaaaatgc cagtcgaact gacccgcaag agcttcaccc cgtacgcagc gaagaagggc
     2461 gaggagtaca tgaacgcccg ccagcgctcg cactttcgca agatcctcga gtcgctgaaa
     2521 gccgagttgt cggaggagat cgaccgcacc gtgcacacca tgcaggacga ggccacggtg
     2581 ttcgccgatc ccaacgaccg cgcaagccag gaatcggaca tggcgctcga gctgcgcaac
     2641 cgcgatcgcg agagaaagct gatcaagaag attgacgaga ccatcgcgcg catcgacgcg
     2701 aacgaatacg gctactgcga cagctgcggg gtggagatcg gcctcaagcg cctggaagcg
     2761 cgtccgacgg cgacactctg catcgactgc aagacgctcg acgagctgcg cgaacgccag
     2821 gtcgccaagt agcgaaaact gctatccgct ctggttcgcg ggctgttcct gttccttcac
     2881 ggctttcatg gacagccgca cgcgtccctt gtcgtcggct tccagtacct tgaccttcac
     2941 ctgctggcct tccttgaggt gatccgaaac ggcgtttacg cgctcatgcg agatctgcga
     3001 gatgtgcagc aagccgtcgc gcccgggcag tagctgcacg atggcaccga agtcgagcag
     3061 gcgtagcacc gtgccctcgt agaccttgcc gacttcgacg tcggcggtga gctcctggat
     3121 gcgctttttc gcggcctcgc caccctcggc cgacacgcac gcgatcgata ccgtgccgtc
     3181 gtcctcgatc tcgatggtgg tgccggtctc ttcctgaatg ccgcggatca cgacgccgcc
     3241 cttgccgatg acgtcgcgga tcttgtcggg gttgatcttg atcttgagga tgcgcggcgc
     3301 gtagctggaa agctcgctgc gcgagccctc gatcgcctgc ttcatgatct cgaggatgtg
     3361 cagccgccct tcacgcgcct ggtcgagcgc caccttcatg atgcccttgt tgatcgactc
     3421 gatcttcagg tccatctgca gcgcggtaat gcccttgtcg gtgcccgcca ccttgaagtc
     3481 catgtcgccg aggtggtcct cgtcgccgag gatgtcggac agcaccgcga agcggttgcc
     3541 gtccttgatc agccccatgg cgatgcccgc cacgtgcgcc ttgaccggca cgccggcgtc
     3601 catgagtgcg aggctgccac cgcacaccga ggccattgac gaagagccgt tcgactcggt
     3661 gatctcggac acgacgcgga tcgagtagcc gaactcctcc ggcgtcggca gcaggccgac
     3721 cagcgcgcgc ttggcgagac gcccgtggcc gatctcgcgc cgcttgggcg gccccacccg
     3781 gccggtctcg ccggtcgcgt acggcggcat gttgtagtgg agcatgaagc gctcgcggta
     3841 ctcgcccgaa agcgcatcaa tgatctgctc gtcgcggcca gtgcccagcg tcacggtgac
     3901 gagcgcctgg gtttccccgc gggtaaacaa ggcagagcca tgagtgcgcg gcaggacgcc
     3961 cacccgtacg ttgatcggcc gcaccgtgcg cgtatcgcgc ccgtcgatgc gcggctcgcc
     4021 gtcgaggatc tggctgcgca cgatcttcga ctcgaggttg aagaacgttt ccttgaccac
     4081 gttgacgtcc ccctgagggt tctcggcgag cagcttgtcg agcgcggtct tgcggatctc
     4141 cgcgagccgc gtattgcgcg cctgcttctg cttaagggcg aacgcggcgc ggaagtcctg
     4201 ctcgaccagc tcggcgaccc ggccgatcag cgcctcgttc ttcgccggca gctgccagtc
     4261 ccacgcaggc ttgccggcct gctcggcgag ctcgtggatc gcctggatcg cgctctgcat
     4321 ctgctggtgg ccgtacatca ccgcgccgag catcgtgtcc tcgggcagct cgagtgcctc
     4381 ggactcaacc atcatcacgc cctgctcggt gccggcgacg acgaggttca gcttcgactc
     4441 cttgagctgc gacgcggtcg ggttgagcac gtaatcgccg ccgacgtagc cgacgcggca
     4501 ggcgccgatc ggaccgttga acgggatgcc ggagatctcg agcgccgccg agaccgcgag
     4561 catcgccggg atgtcggaat cgatctccgg gttgagcgac agcaccgtgg cgacgacctg
     4621 cacttcgttc aggaagccgt ccggaaagag cggccggatc ggccggtcga tgagccgcga
     4681 ggtcagcgtc tccttttccg acggccggcc ctcgcgcttg aagaaaccgc ccgggatctt
     4741 gccggcagcg taggtcttct ccacgtagtc gaccgttagc ggaaaccagt cttggccggc
     4801 gcgcgcctcc ttggccgcga ctaccgtggc gaggacgacg gtgtcgtcca tcgagcagat
     4861 caccgagccg tgcgcctggc gcgcgatttc gccggtctcg aaagtgacct ggtgggctcc
     4921 gtaggaaaag cttttcttta tggggttcac gggaatcctt gaaagcaaaa agcgctcatg
     4981 cggcgatgag cgctcgactt gacggcattg gccgggagtc tatttgcgga ggctcagccg
     5041 ctcgatgagg ccgcggtact tgtccgcgtc cttgcgcttg aggtaatcga gcagcttgcg
     5101 gcgctggctc accagcttca gtagcccgcg gcgcgaatgg aaatccttaa cgtgggtctt
//
LOCUS       DASTPD010000122         8855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_268371, whole genome shotgun sequence.
ACCESSION   DASTPD010000122 DASTPD010000000
VERSION     DASTPD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268371"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VFR57_04310"
     CDS             <1..1340
                     /locus_tag="VFR57_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0258629.1"
                     /translation="VGGCDKTVPAQLMGAASADVPAVQLLAGPMMPTSFQGERLGACT
                     DCRRFWALHRAGKVDSSEIETIEGNLATTAGTCAVMGTASTMASIAEALGMALPGTAA
                     IPAVHADRLRAAEASGRRAAEMAHSPLKPSQIITEKSVENALRVLLAIGGSTNAIVHL
                     SAIAGRVGIHIDLKKLNALSDSTPVLVDLKPTGQHYMSDLYAAGGVGAVMRELKPLLH
                     LDCMTVGGISLKEVLEKQPAWVDRTVVKKFEEPLASQGGLVALFGSLAPNGAILKRSA
                     ADPKLFEKEGRAVVFKSLDDLSERIDDPALDVTADDFLMLQNAGPKSGYAMPEAGYLP
                     IPAKLARAGVKDMVRISDARMSGTAYGTIVLHVSPEAAVGGPLALVRNGDRIRLSVKE
                     RRIDLLVDENEIKKRRSGWKAPVAAPARGYAKLYMDHVLQAEHGCDFDFLRKA"
     gene            1427..1930
                     /locus_tag="VFR57_04315"
     CDS             1427..1930
                     /locus_tag="VFR57_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258630.1"
                     /translation="MAPLANPPLTIAHRFVESEAGLAYYARRPDGLMWRRKELFDTPV
                     YYLAFHGRPGGVNPLLGRIEADALCEAFANYGGYTNLAYFACCNVLRGEKGRRFARNF
                     LRISGMRAVIGYATTVDWMESLVADMLFLHRFYRDPAPWRNLKQIFRSVQRDYPLARR
                     LGHTLIT"
     gene            complement(1923..2612)
                     /locus_tag="VFR57_04320"
     CDS             complement(1923..2612)
                     /locus_tag="VFR57_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0258631.1"
                     /translation="MRTFRAALDLLGAMSEAALDKVSFAKLGVERLPALVASEFTTLS
                     ICHLASGKREVFGLPAGALSAQDRAAFDRHFHEHPLVRFHAYQGGRVPQRITDSVPFE
                     QFRRTALYNDYYRRIRIDHAIALPIYVRDGLLVSFVLNRTRRDFTERERALLEVLRPH
                     LAKIYQRISTVGQLTARETEVLRWVAAGKSNAQIGAILRISARTVQKHLQNIYDKLGV
                     ESRTAAAMRVK"
     gene            2679..3038
                     /locus_tag="VFR57_04325"
     CDS             2679..3038
                     /locus_tag="VFR57_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0258632.1"
                     /translation="MEAAERKDFRKPDEVREFPKGRLELIKIGGATVGRAVFEPGWRW
                     STSVQPLVNTKSCEAPHFQYHVSGVLRIKMDDGTEFDCKPGEVSLLPSGHDAWVVGNE
                     PAVVVDFQGMIDYARSR"
     gene            3047..3427
                     /locus_tag="VFR57_04330"
     CDS             3047..3427
                     /locus_tag="VFR57_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0258633.1"
                     /translation="MLARAEVTVILPVKELERARDFYVNRLGLEAEGLAPDGKFILRA
                     KGTRLALMPKPGGTKAEHTALSFKVDDIAAEIQALKARGVSFHDYDLPGFKTVNHVAV
                     LGKEKAAWFSDPEGNILCLHEDIA"
     gene            3424..4011
                     /locus_tag="VFR57_04335"
     CDS             3424..4011
                     /locus_tag="VFR57_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0258634.1"
                     /translation="MIYHEGARRLQDRFDSRRLADRLVEKLWRTAFTAEDKAFIEASP
                     MFFLATADASGQPDCSYKGGLPGFVRVTGPSELSFPSYDGNGMFRSLGNVLANPRVGM
                     LFLDFEQPRRLRVNGHASLVDDVSGFEGAQLVVRIAADAIFPNCPRYIHRYRLLETSV
                     YAPRPGYEPPAPGWKKNFSDVLPHGRKGDQEEPAD"
     gene            4065..4532
                     /locus_tag="VFR57_04340"
     CDS             4065..4532
                     /locus_tag="VFR57_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0258635.1"
                     /translation="MDREFIARREDHSALRIWLRLLTCTQLIERRVRSGLREEFGTTL
                     PRFDLMAQLERHREGLKMNELSRLLMVTGGNVTAIVDQLEKEGLVERLDEPDDRRAFR
                     IRLTPSGEKGFAEMARAHEQWIVELLGGLPRREQAELLRLLAKVKVHAVEVTS"
     gene            4644..5750
                     /locus_tag="VFR57_04345"
     CDS             4644..5750
                     /locus_tag="VFR57_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0258636.1"
                     /translation="MDRFVHYEWPFFEPKHAALAREIEGFAKDNLGHAHGQDADAICR
                     RTVQDLGCAGFLRHSVCERPDVRSIAIVREVLAYHAGLADFSFVMQGLGSGPIALAGD
                     SSLKQKYLPAAARGDAIAAFALSEPDAGSDVQAMATRAERRGGVWVLNGTKTWISNGG
                     IADFYVVFAKAAEGISAFVVDAKELDASERIEIVAPHPMAVLKLKDSKAVLLGEPGQG
                     FKLAMRNLDIFRTTVGAAALGFSRRALQESLSRSRQRRIFGQALSDFQMTQAKLADMA
                     TRIDAGALLVYRSAWKRDVQNARVTREAAMAKMFATESAQSVIDDAVQICGGLGVMRG
                     HPVERLYREVRALRIYEGATEVQKLIISRELLKV"
     gene            5747..7360
                     /locus_tag="VFR57_04350"
     CDS             5747..7360
                     /locus_tag="VFR57_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU0258637.1"
                     /translation="MTYTAHLDTFARDNLPPKSEWPEFIFELPELQYPGRMNCATELL
                     DKPVTRGHGHRVALRTDDGECTYTQLFTQANRLANVLVREMGLRPGNRVLLRGPNNPM
                     MAAAWFAVMKAGGVCVATMPLLRAKELTDVITKAEVTHALCDKRLDEELKLAQAACRS
                     LKSIKYWYDDGAGSVDELVMKQPLWFTNVATAADDVALIAFTSGTTGKPKGTIHFHRD
                     VVAMCDCFPRSILQAGKDDIFCGTPPLAFTFGLGGLLCFPMRIGASSVLVEKHTPETL
                     LDTIQRFKATVCFAAPVMYRGMAAIADRFDLGSLRRCVSAGEALPDATRQAFKQASGI
                     EIIDGIGSTEMIHIFISHTPERVRRGATGYAIPGYRAAVLDEDGRPCAPGKVGRLAVK
                     GPTGCRYLADDRQANYVRNGWNITGDAYMVDSDGYFFYQARTDDMIISAGYNIAGPEV
                     EGALLQHPAVAECGVIGWPDEDRGTIVKAFVVLKPGNSRGDQTARQLQEHVKKVIAPY
                     KYPRAIEFVDALPRTETGKLQRFKLRKPA"
     gene            7357..7761
                     /locus_tag="VFR57_04355"
     CDS             7357..7761
                     /locus_tag="VFR57_04355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0258638.1"
                     /translation="MMQILQPPGWARPKGFSNGVFCKGNLVFIAGQIGWTGEGRWEAH
                     DFAGQFRQALANVIAVLKEAKGRPEHIARLTWYVVDKNEYLGSLKAVGEAYRELMGRH
                     YPTMAVVQVSGLVEPEARLEIEATAVVPETPS"
     gene            7758..>8855
                     /locus_tag="VFR57_04360"
     CDS             7758..>8855
                     /locus_tag="VFR57_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0258639.1"
                     /translation="MKVSVIGGGPGGLYFALLAKKAWPRWDITVYERNRPDDTFGFGV
                     VFSDQTLDTFKAYDLVTYERIRRRFAYWGDVDVVYKGQVMRSGGNGFCGCSRVALLNI
                     LQDRGRELGVHMRFQREVDGLEEFADSDLIVVADGINSRIREKHKEHFEPSVDLRPNK
                     FTWLGSTKPLDAFKYFFRETPQGVLLAHCYQYEADRSTWVIEMNEDTWRNFGFDKLSE
                     TEMLPVLERVFAEELDGHPLIANRSLWRNFPNITNKTWVRGNAVLVGDAKATAHFSIG
                     SGTKLAMEDAIALFEAFREHSDVRKALAVYDTARREEVEKTQHAANVSLAWFEHMKRY
                     WGMEPVQFAFGVMSRSKQITWENLELRDPRFI"
BASE COUNT         1632 a         2771 c         2935 g         1517 t
ORIGIN      
        1 tggtcggcgg ctgcgacaag accgttcccg cgcagctcat gggcgccgcg tccgcggacg
       61 tcccggcggt gcagcttctc gccggcccga tgatgcccac ttccttccag ggcgagcggc
      121 tcggcgcctg caccgactgc cggcgcttct gggcgctcca tcgagccggg aaagtagatt
      181 cttccgaaat agaaactata gaaggcaatc tcgccaccac cgccggtacc tgcgcggtaa
      241 tgggcacggc atcgaccatg gcctcgattg ccgaggcgct cggcatggcg cttcccggca
      301 ccgcggccat tcccgcggtg cacgccgatc ggctgcgcgc cgcggaagca agcggcaggc
      361 gcgcggcgga aatggcccat tcgcccctca agccgagtca aatcatcacc gaaaaatccg
      421 tagagaacgc gctgcgcgtg ctgctcgcca tcggcggctc gaccaacgcg atcgtgcacc
      481 tgagcgccat tgcaggacgg gtgggaattc atattgatct gaagaaactc aatgccctgt
      541 cggacagcac gccggtcctg gtcgacctga agccgaccgg gcagcattac atgtcggacc
      601 tctacgcagc gggcggcgtg ggcgcggtca tgagggagct caagccgctc ctccacctgg
      661 attgcatgac cgttggcggc atttctctga aggaagtgct ggaaaaacag ccggcgtggg
      721 tggaccgcac agtagtgaag aaattcgagg agcccctcgc aagccaagga gggctggtcg
      781 cgttgttcgg ctcgctcgcg cccaatggcg cgatcctcaa gcgctccgcg gcggacccga
      841 agctgttcga gaaggaaggg cgcgcggtgg tgttcaaatc gctcgacgat ctttccgagc
      901 ggatcgacga tcctgcgctc gacgtgaccg ctgatgattt tctaatgctg cagaacgccg
      961 gcccgaagtc gggctacgcc atgccggagg cgggctatct gccgattccg gccaagctcg
     1021 ctcgtgccgg cgtgaaggac atggtgcgga tctccgacgc gcgcatgagc ggcaccgcct
     1081 acggcaccat cgtgttgcat gtttctccgg aagcggcagt cggcggaccg ctcgcactgg
     1141 tccgcaatgg cgaccgtatc cggctttccg tgaaagaacg gcggattgat ctgcttgtgg
     1201 acgagaacga aatcaaaaag agaagaagcg gctggaaggc tccggtcgcg gcaccagccc
     1261 gcggctacgc taagctctac atggaccacg tgctccaggc cgagcacggc tgcgatttcg
     1321 attttctgag aaaagcctga gagcgccgcc gaagctcgtt tgcctggagg cgtactggaa
     1381 cgagaagctg ttccagagct tctcggtcaa agggttcttc gaggcgatgg cgccgctcgc
     1441 caaccccccg cttaccatcg cacaccgctt cgtcgagtcc gaagcgggtc ttgcctatta
     1501 cgcgaggcgg ccagacggcc tgatgtggcg caggaaggag ctcttcgata cgccggtgta
     1561 ctacctcgcg ttccacggca ggcccggcgg cgtaaacccg ctcctcgggc gcatcgaggc
     1621 cgacgcgctg tgcgaggcat ttgcaaacta cgggggctac acgaacctcg cttacttcgc
     1681 ctgctgcaac gtgctgcgcg gagagaaggg gcggcgcttt gcgcgaaatt tcctcaggat
     1741 ctcgggcatg agggcggtga tcggctacgc caccacggtc gactggatgg aaagtctggt
     1801 ggccgacatg cttttcctgc accgcttcta ccgcgatccg gcgccctggc gaaatctgaa
     1861 gcagatcttc cgttctgtgc agcgcgacta tccgctcgct cggcgtctag gtcatacgtt
     1921 gatcacttga cccgcatcgc ggccgcggtg cggctctcca ctccgagctt gtcgtagatg
     1981 ttctgcaggt gcttctgcac cgtgcgcgcg ctgatgcgca ggattgcgcc gatctgcgcg
     2041 ttgctctttc cggcggctac ccagcgaagc acctcggttt cccgcgccgt gagctgaccg
     2101 acggtgctga tcctttgata gatcttggcc aggtgcggac gcagtacctc gagcagcgcg
     2161 cgctcgcgct cggtgaagtc gcgccgcgtg cggttcagca cgaagctcac gagcaggccg
     2221 tctcgcacgt agatcggcag cgcgatcgcg tggtcgatgc ggatgcggcg gtaatagtcg
     2281 ttgtaaagcg cggtgcggcg gaattgctcg aacgggaccg agtcagtaat gcgctgcgga
     2341 accctgccgc cctggtaggc gtgaaagcgc accagcggat gctcgtggaa atggcggtcg
     2401 aaggcggcgc gatcctgggc gctcagcgcg ccagccggca ggccgaacac ctcgcgcttg
     2461 ccgctcgcca ggtggcagat cgataatgtg gtgaactcgg aagcgaccag cgccgggagc
     2521 cgctcgacgc cgagcttcgc gaacgagacc ttgtccagcg ctgcctcgct catggcgccg
     2581 aggagatcga gggctgcgcg gaaagtacgc atatttgcgt atgtagcctg ccgcaaggct
     2641 gagccaaagt acaggcgcta ttgccacagg agactgctat ggaagccgca gaacggaagg
     2701 attttcgcaa gccagacgaa gtccgcgagt tcccgaaggg gcgtctggag ctgatcaaga
     2761 tcggaggcgc gaccgtgggg cgcgcggtgt tcgagccggg ctggcgctgg tccacttcgg
     2821 tgcagccgct cgtcaataca aagagctgcg aggcgccgca cttccagtac cacgtctctg
     2881 gggtcctcag gatcaagatg gatgacggca ccgagtttga ctgcaagcct ggtgaagtct
     2941 cgctgctgcc ttccggtcac gatgcctggg tggtgggaaa cgagcccgcg gtcgtggtcg
     3001 acttccaggg gatgatcgac tacgcgaggt ctcgatgagc cggccgatgc tcgcccgcgc
     3061 cgaggtgacc gtcatccttc cagtgaagga gctcgagcgt gcgcgcgatt tctacgtgaa
     3121 ccggctcggc ctggaggctg aggggctggc gcccgacggc aagttcatcc tgcgcgcgaa
     3181 gggcacgcgc cttgcgctca tgcccaagcc gggcggcacc aaggcggagc acaccgcgct
     3241 cagcttcaaa gtggacgaca tcgccgcgga gatccaggct ctgaaggccc gcggcgtgag
     3301 ctttcacgac tacgacctgc cgggcttcaa gaccgtgaat cacgtcgccg tgctcggaaa
     3361 agaaaaggca gcctggttca gcgaccccga gggcaacatc ctctgcctgc acgaggatat
     3421 tgcttgattt accacgaggg ggcccgtcgg ctgcaggacc gcttcgacag ccggcggctc
     3481 gccgaccggc tggtggagaa gctctggcgc accgcgttca cggcggaaga caaggccttc
     3541 atcgaagcga gccccatgtt cttcctcgcg accgcggacg cgtccgggca gcccgattgc
     3601 tcctacaagg gcgggctgcc gggtttcgtg cgggtgaccg ggccttccga gctgtcattt
     3661 ccgagctacg acggcaacgg catgttccgc agcctcggca acgtactcgc caacccgcgc
     3721 gtcgggatgc tgttcctcga cttcgagcaa ccgaggcgcc tgcgggtcaa cgggcacgca
     3781 agcctggtgg acgacgtttc agggttcgag ggcgcccagc tcgtcgtccg gatcgcggcc
     3841 gacgcgatct tccccaactg cccgcgctat atccaccgct atcgcctgct cgagacctcg
     3901 gtatatgcgc cgaggccggg gtatgagccg cccgcgccgg gctggaagaa aaacttcagc
     3961 gacgtcttgc cgcacggccg caaaggcgac caagaagagc cggccgattg acaagccagt
     4021 cgatattact tcaagcttga tctattcagg cttggagtac cggcatggac cgagaattca
     4081 tcgcgcggcg cgaggaccat agcgcgctgc ggatctggct gcgccttctc acctgcaccc
     4141 agctcatcga acggcgcgtg cgctcgggtt tgcgcgagga gttcggcacc acgctgccgc
     4201 gcttcgatct gatggcgcag ctcgagcgcc atcgcgaggg gctcaaaatg aacgagctct
     4261 cgcgcctgct gatggtcacc ggcggcaacg tcaccgcgat cgtcgaccag ctcgagaagg
     4321 agggcctggt cgagcggctc gacgagcccg atgaccggcg cgcattccgc atccgcctga
     4381 cgcccagcgg cgagaagggc ttcgccgaga tggcgcgcgc gcatgagcag tggatcgtcg
     4441 agcttctcgg cggcctcccc cgccgcgagc aggccgagct cttgaggctc ctcgccaagg
     4501 tgaaagtcca cgcggtggag gtcacttcat gaagacgccg aagcatttcc tctggactca
     4561 ggccgagcca tgctgacgaa ggacttgcac cgcgcccacg aagccttcgt cgccaagcgt
     4621 aagcccgttt tcgaggggga ttgatggacc gatttgtgca ctacgagtgg cctttcttcg
     4681 agccgaagca cgctgcgctc gcgcgcgaga tcgaaggttt tgccaaggac aatctcggcc
     4741 atgctcatgg acaggacgcc gatgccattt gcagacgaac ggtgcaggac ctcggctgcg
     4801 cgggcttctt gcgccactca gtgtgcgaga ggcccgacgt gcgatcgatc gcaatcgtgc
     4861 gcgaggtgct cgcttaccac gcggggctcg ccgacttctc cttcgtgatg cagggtctcg
     4921 gcagcgggcc gatcgcgctc gcgggtgatt cttcattgaa gcagaaatat ctgccggcgg
     4981 cggcgcgcgg cgacgcgatc gcggcttttg cgttgtcgga gcccgacgcc ggctcggatg
     5041 tgcaggcaat ggcgaccagg gccgaacggc ggggtggcgt ctgggttcta aacgggacga
     5101 agacctggat ttcgaacggc gggatcgcgg acttctacgt cgtgttcgcg aaggccgcgg
     5161 aaggcatcag cgcgttcgta gtcgatgcga aggagctcga cgcctccgag cgcatcgaga
     5221 tcgtcgcgcc gcaccccatg gctgtattga aattgaaaga cagcaaagcg gttctgctag
     5281 gcgaacccgg acaggggttc aagctggcga tgcgcaacct cgacatcttc cgcaccacgg
     5341 tgggcgctgc cgcgctcgga ttctccaggc gggcgctcca ggagtcgctt tccagatccc
     5401 gccaaaggag aatattcggc caggcgctgt cggatttcca gatgacccag gcgaaactcg
     5461 ccgacatggc gacgcgcatc gacgcgggcg cgctgctcgt ctaccgctcg gcctggaagc
     5521 gggatgtcca aaacgcgcgc gtcacgcgcg aagccgccat ggcgaagatg ttcgccaccg
     5581 aatcggcgca gagcgtgatc gacgacgcgg tgcagatctg cggcggcctg ggcgtgatgc
     5641 gcgggcaccc ggtcgagcgc ctgtaccgcg aggtgcgcgc tctgcgcatc tacgagggcg
     5701 cgaccgaggt gcagaagctg atcatatcga gagagttgtt gaaggtatga catataccgc
     5761 gcacctcgat actttcgcgc gcgacaacct gccgccgaag agcgagtggc ccgagttcat
     5821 cttcgagctg ccggagctcc agtatcccgg ccgcatgaac tgcgccacgg agctcctcga
     5881 caagccggtg acgcggggcc acggccaccg cgtcgccctg cgcacggacg acggcgagtg
     5941 cacctacacg cagctcttta cgcaggcgaa ccgcctcgcc aatgtgctgg tgcgcgagat
     6001 gggcctcagg cccggcaatc gcgtcctcct gcgcgggccg aacaacccga tgatggcggc
     6061 ggcgtggttc gcggtaatga aggcgggcgg cgtgtgcgtt gcgacgatgc ccctgctgcg
     6121 cgcgaaggag ctcaccgatg tcatcacgaa agctgaagtc acgcatgcac tgtgcgacaa
     6181 gcggctcgac gaggaattga agcttgctca ggctgcctgc cgttcattga aaagcatcaa
     6241 gtactggtac gacgacgggg cgggctcggt cgacgagctg gtgatgaagc agccgttgtg
     6301 gtttacgaac gtggcgaccg cagccgacga cgtcgcgctc atcgcattca cctcgggtac
     6361 caccggcaag cccaagggca cgatccactt tcaccgcgac gtggtggcga tgtgcgactg
     6421 ctttccgcgc tcgattctgc aggccggcaa ggacgacatc ttctgcggca cgccgccgct
     6481 cgccttcact ttcggcctcg gcggcctgct ctgcttcccg atgcgcatcg gcgcatccag
     6541 cgtgctggtc gagaagcaca cgccggagac gcttctcgac accatccagc gcttcaaggc
     6601 gacggtgtgc ttcgccgcgc cggtgatgta ccgcggcatg gcggccatcg cggaccggtt
     6661 cgacctgggc tccctgagga ggtgcgtgtc cgcgggagaa gcgctgcccg acgctacgcg
     6721 ccaggcgttc aagcaggcga gcggcatcga gatcatcgac ggcatcggct cgaccgagat
     6781 gatccacatc ttcatttcgc acacgcccga gcgcgtgcgg cgcggcgcca cgggctacgc
     6841 gatcccgggc taccgcgccg ccgtgcttga cgaagacggc aggccgtgcg cgccgggcaa
     6901 ggtgggcagg cttgcggtca aggggccgac cggctgccga tacctggccg acgaccggca
     6961 agcgaactac gtcaggaacg gctggaacat cacgggcgat gcgtacatgg tggactcgga
     7021 cggctacttc ttctaccagg cacgcaccga cgacatgatc atatcggcgg gctacaacat
     7081 cgcgggcccg gaggtcgaag gcgcgctgct ccagcatccc gcggtcgccg agtgcggagt
     7141 gatcggctgg cctgacgaag accgcggaac gattgtcaag gctttcgtgg tcctgaagcc
     7201 gggcaattca cgcggagatc agacggcgcg ccagctgcag gaacatgtaa agaaggtcat
     7261 cgcgccctac aagtacccgc gtgccatcga gttcgtcgat gcgctgccgc gcaccgagac
     7321 tggcaagctg caacgcttca agctgaggaa acccgcatga tgcagattct gcagccgccc
     7381 ggctgggcgc gacccaaagg cttctccaat ggagttttct gtaagggaaa ccttgtgttc
     7441 attgcgggcc agatcggctg gaccggcgag gggagatggg aggcgcacga tttcgccggc
     7501 cagtttcgtc aggcgctcgc gaacgtcatc gcggtgctga aagaagcgaa aggcaggccg
     7561 gagcatatcg cgaggcttac gtggtacgtg gtcgacaaga acgagtacct cggctcactg
     7621 aaggccgtcg gagaagcgta ccgcgagctc atgggccggc actacccgac gatggcggtg
     7681 gtgcaggtga gcgggctcgt ggagcccgag gctcgcctcg agatcgaagc caccgccgta
     7741 gtaccggaga cgccatcatg aaagtcagcg tgatcggagg aggaccgggc ggcctgtact
     7801 tcgcgctgct cgctaagaaa gcctggccgc gctgggatat cacggtgtac gagcgcaacc
     7861 gccccgacga cacgttcggc ttcggcgtgg tgttctcgga ccagacgctc gacaccttca
     7921 aggcctacga cctcgtcacc tacgagcgca tccggcgccg cttcgcctac tggggcgacg
     7981 tcgatgtggt ctacaagggg caggtgatgc gctcgggcgg caacggcttc tgcggctgct
     8041 cgcgggtggc gctgctcaac atcctgcagg accgcgggcg cgagctcggc gtgcacatgc
     8101 gctttcagcg cgaggtggac gggctggaag agttcgccga ctcggacctg atcgtggtcg
     8161 ccgacggcat caactccagg atccgggaga agcacaagga gcacttcgag ccgagcgtgg
     8221 atctgcggcc caacaaattc acctggctcg gatcgaccaa gccgctggac gcgttcaagt
     8281 atttcttccg cgagacgccg cagggcgtgc tgcttgcgca ctgctaccag tacgaggccg
     8341 accgctctac ctgggtgatc gagatgaacg aggacacctg gcgcaacttc ggcttcgaca
     8401 agctttcgga gaccgagatg ctgcctgtgc ttgagcgagt gttcgccgaa gagctcgacg
     8461 ggcacccgct gatcgccaat cgctcgctgt ggcggaactt ccccaacatt acgaacaaga
     8521 cctgggttcg tggcaacgcg gttctcgtcg gggatgccaa ggcgacggcg catttttcca
     8581 tcggctcggg gaccaaactc gccatggaag atgcgatcgc gctctttgaa gccttcagag
     8641 aacactccga tgtaagaaaa gccctggccg tttacgacac agcgcggcgc gaggaggtag
     8701 agaagaccca gcacgcggcg aacgtttcgc tcgcgtggtt cgagcacatg aagcgctact
     8761 ggggcatgga gccggtgcag ttcgccttcg gcgtgatgtc gcgctccaag cagatcacct
     8821 gggagaacct ggagctgcgc gacccgcgct tcatc
//
LOCUS       DASTPD010000123         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1494184, whole genome shotgun sequence.
ACCESSION   DASTPD010000123 DASTPD010000000
VERSION     DASTPD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494184"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /gene="ileS"
                     /locus_tag="VFR57_04365"
     CDS             complement(<1..1976)
                     /gene="ileS"
                     /locus_tag="VFR57_04365"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU0258640.1"
                     /translation="MADYKDTLNLPETPFPMRGDLAKREPQWVREWQEKGVYRRLRAV
                     ARGRPRFVLHDGPPYANGDIHIGTAVNKILKDIIVKSKTLAGFDAPYVPGWDCHGMPI
                     EVQIEKKYGKNLPTAETLRLCRAFAAEQIERQKKDFQRLGVLGGWDSPYTTMAPRTEA
                     EEIRVLGRLLEKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDAAIDVAFEVGEQDL
                     LKLWSAFGSKAASNKPIYAVVWTTTPWTLPANQALNVHPDFSYDLVDTEKGLLILATD
                     LKEACLNRYGLSGTTVGQCKGSALENIRFRHPFYEREAPVYLGDYVTLETGTGIVHSA
                     PAYGVEDFESCRRYGMRDEQIMAPVMGDGRFAPSLELFGGMSVWDANPKIVKTIEDRG
                     ALLAVDRKHTHSYMHCWRHKTPVILRATTQWFASMDKPVGGESLRQTALRGIEDTQFF
                     PSWGKARLHGMIANRPDWTLSRQRQWGVPMPFFLHRETGALHPRTPELLEEVAKRVEK
                     EGIEAWQTLRAEELIGNDSKDYEKSRDTLDVWFDSGSTHCTVLRGSHRAESEFPADLY
                     LEGSDQHRGWFHSSLLVSCMLDGVPPYKGLLTHGFVIDLEGRKMSKSKGNTMAPQEIS
                     NTLGAEILRLWVASSDYSSELNLSSEILKRVVES"
     gene            complement(1969..2871)
                     /locus_tag="VFR57_04370"
     CDS             complement(1969..2871)
                     /locus_tag="VFR57_04370"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0258641.1"
                     /translation="MVVTHGSLQRARGRCAATIGNFDGVHRGHRALIDRVTAKAREAK
                     LTACVLTFEPHPREFFTPAAAPARITRLRDKLELMAQAGVEHVHVARFDARFAAMPAE
                     RFIRDVLVRGLGVEWLLVGRDFRFGARRAGDFTALKNQGFALEAMPDVELGGVRVSSS
                     AVRAALKAGELTEAERLLGHPYTISGRVAHGAKLGRGLGFPTANIVLRRAPPLAGIFV
                     VEVDRYGPGVASIGKRPTVNPVTIPLLEVHLFDHEADLYGEHLRVRFLKKLRDEEKYD
                     GLPALQAAIARDARQAREFFLSHG"
     gene            2919..3494
                     /locus_tag="VFR57_04375"
     CDS             2919..3494
                     /locus_tag="VFR57_04375"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEU0258642.1"
                     /translation="MKSIEDQMSFYAAYHQDARNKATHFIGVPAIMLSLFIPLAWIRL
                     EVGGFVLTAAMLFAAVVLVYYFVLDVPLGLAMLVVTAILLWIAGGIASLGAAAGWTWF
                     AILFVGGWILQLVGHVFEGRKPALADNLFQIFVAPMFLAAEVFFALGYKRALQRRVNE
                     KAQLSRAQSAESSISLERTSSRSGASGSRSA"
     gene            complement(3404..>4076)
                     /locus_tag="VFR57_04380"
     CDS             complement(3404..>4076)
                     /locus_tag="VFR57_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0258643.1"
                     /translation="AEERVVALNQFLYDDLGYWGNTEDYYDPRNSYLNEVIERKTGSP
                     ITMSILYMELGRRIGLPLEGVSFPGHFLVRLPLRGGMLVLDPFAGGAPQSEDELRSRV
                     KRVIPDGVADDLPAAELPLDQFLEPATNRQILSRVLRNLKAIYRDSDKPERMLDVLNR
                     MLLVTPDASAELRDRGYIYQRLECYRAALKDLSDYAEREPDAPDLDDVRSRLMELSAL
                     CARLN"
BASE COUNT          680 a         1341 c         1284 g          771 t
ORIGIN      
        1 tagctttcca caacgcgctt caggatctcg ctggagagat tcagctcgct cgaataatcg
       61 ctgcttgcca cccacagccg caggatctcg gcgccgagcg tgttcgagat ctcctggggc
      121 gccatcgtgt tgcccttgga cttcgacatc ttgcggcctt cgaggtcgat gacgaagccg
      181 tgggtcagca ggcccttgta cggcggcacc ccgtccagca tgcaggaaac cagcagcgat
      241 gaatggaacc agccgcgatg ctggtccgag ccctcgagat atagatcggc cgggaactcc
      301 gactccgcgc gatgcgagcc gcgcagcacg gtgcagtgcg tcgagcccga gtcgaaccag
      361 acgtccagcg tgtcgcggct tttctcgtaa tccttgctgt catttccgat caattcttcg
      421 gccctgagcg tttgccaggc ctcgatgcct tccttctcga cgcgcttcgc cacctcctcc
      481 agcagttccg gcgtgcgcgg atgcagcgcg ccggtctcgc gatggaggaa gaaaggcatc
      541 ggcactcccc attgccgctg gcgcgacagc gtccagtccg gccggttggc gatcatcccg
      601 tgcaggcgcg ccttgcccca ggacgggaag aactgcgtgt cctcgatgcc gcgcaacgcg
      661 gtctgccgca ggctttcacc gcccacgggc ttgtccatcg acgcgaacca ttgcgtggtg
      721 gcgcgcagga tcaccggcgt cttgtggcgc cagcagtgca tatagctatg cgtgtgcttc
      781 cggtcgaccg caaggagcgc gcccctgtct tcgatcgtct tgacgatttt cgggttggca
      841 tcccacaccg acatcccacc gaaaagctcg agcgaaggcg cgaagcggcc gtctcccatc
      901 accggcgcca tgatctgctc gtcacgcatg ccgtagcgcc ggcacgactc gaaatcctcc
      961 acgccatagg cgggcgcgga gtgcacgatc ccggttccgg tctcgagggt gacgtagtcg
     1021 ccgagataga caggtgcctc gcgctcgtag aacggatgac ggaagcggat gttctccagc
     1081 gcgctgcctt tacattgccc caccgtagtt cctgagaggc catagcggtt caagcaggcc
     1141 tctttcagat ccgtggccag aatgagcagg cccttctccg tgtcgaccag gtcgtagctg
     1201 aaatcgggat gcacattgag tgcctggttc gcgggcagcg tccacggcgt cgtggtccag
     1261 acgacggcat agatcggttt gttcgaagca gccttgctgc cgaacgccga ccaaagcttc
     1321 agcagatctt gctctccaac ttcaaacgca acatcgatcg ccgcgtcttt gcggtcctcg
     1381 tactccactt ccgcctcggc gagcgccgag ccgcagtcga agcaccagtt cacgggcttg
     1441 agaccacggt acacgtagcc cttctccagc agccggccga gcacgcggat ttcctccgcc
     1501 tcggtgcgcg gtgccatggt cgtgtacggg ctgtcccagc cgccgagcac gccgaggcgc
     1561 tgaaagtcct tcttctgccg ctcgatctgc tccgcggcga aggcgcggca tagccgcagc
     1621 gtctccgccg tcggcaggtt cttgccgtac ttcttctcga tctgcacctc gatcggcatg
     1681 ccgtggcaat cccagcccgg cacgtagggt gcgtcgaacc cggcgagcgt tttcgacttc
     1741 acgatgatgt ccttcaggat cttgttcacc gcggtgccga tgtggatgtc gccgttcgcg
     1801 taaggcgggc cgtcgtgcag cacaaagcgc gggcgccccc tcgcgaccgc gcgcaggcgg
     1861 cgatagacgc ctttttcctg ccattccctg acccattgcg gctcgcgctt cgcaaggtcg
     1921 ccgcgcatgg gaaacggcgt ttccgggagg ttcagcgtgt ctttgtagtc agccatggct
     1981 cagaaagaat tcacgcgcct gcctcgcgtc gcgcgcgatc gcggcctgca gcgcgggaag
     2041 gccgtcgtac ttttcctcgt cgcgcagctt cttcaggaag cgcacgcgca aatgctctcc
     2101 gtagagatcg gcttcgtggt cgaaaaggtg cacttcgagc agcggaatcg tcaccgggtt
     2161 caccgtcggt cgcttgccga tgctcgcgac gccagggccg taacggtcga cttcgacgac
     2221 aaagatcccg gcgagcggcg gtgcgcggcg cagcacgatg ttcgcggtcg ggaagccgag
     2281 cccgcgaccg agcttcgcgc catgcgcaac gcggccgctg atggtgtagg gatggcccag
     2341 cagacgctcg gcttccgtga gctcgcccgc cttgagggcg gcgcgcaccg ccgagctcga
     2401 cacccgcaca ccgccgagct cgacgtcggg catagcctcg agcgcgaagc cctggttctt
     2461 cagtgccgta aagtcccctg cacgccgggc gccgaaacgg aagtcccttc ccaccagcaa
     2521 ccactcgacc cccaaaccgc gcaccaagac atcccggatg aaccgctcgg ccggcatggc
     2581 ggcgaagcgc gcgtcgaagc gcgccacgtg cacatgctcc acccccgcct gcgccatcag
     2641 ctcgagcttg tcccgcagcc gggtgatgcg cgccggcgcg gcggcggggg taaaaaactc
     2701 gcggggatgc ggctcgaagg tgagaacaca ggctgtgagc ttcgcttcac gtgccttggc
     2761 ggtgacccgg tcgatgagcg cgcgatggcc gcggtgcacg ccgtcgaagt ttccgatcgt
     2821 agccgcacac cggccccggg cgcgctgcaa agagccgtgg gtgacgacca tggacgcggt
     2881 aaaatgctga attatagtcg gtttatgcga gtttgatgat gaagagcatc gaggaccaga
     2941 tgagtttcta cgccgcgtac catcaggacg cgcgcaacaa ggccacgcac ttcatcggcg
     3001 tgcccgcgat catgctttcg cttttcattc cgcttgcctg gatccgcctg gaggtaggcg
     3061 gcttcgtcct taccgccgca atgcttttcg ccgccgtggt cctcgtctat tacttcgttt
     3121 tggacgtccc tctgggcctt gccatgctgg ttgttaccgc gatactgctg tggatcgccg
     3181 gaggcattgc aagcctcggc gccgcagccg ggtggacatg gtttgccatc ctcttcgtcg
     3241 gcggttggat actgcagctc gtgggccacg tgttcgaagg ccgcaagccg gcgctggcgg
     3301 acaacttgtt ccagatcttc gtcgcgccga tgtttctcgc cgccgaggtg ttcttcgctc
     3361 tcggctacaa acgggcgctg caaagaagag tgaatgaaaa agctcagttg agccgggcgc
     3421 agagcgcgga gagctccatc agcctcgagc gcacatcgtc gaggtcgggg gcgtcgggtt
     3481 cgcgctcggc gtaatcgctc aggtccttga gcgccgcccg gtagcactcc aggcgctggt
     3541 agatgtagcc gcggtcgcgc aactccgcgc tcgcatcggg cgtgaccagg agcatccggt
     3601 tcagcacgtc aagcatgcgt tctggcttgt cgctgtcgcg gtagatcgcc ttcaggttgc
     3661 gcagcacgcg cgacaggatc tgccggttgg tcgccggctc gaggaactgg tcgagcggca
     3721 gctccgcggc cggcaggtcg tcggccacgc cgtccgggat cacgcgcttg acgcggctgc
     3781 gtagctcgtc ctcggactgg ggtgcgccgc cggcaaacgg atcgagcact agcatccctc
     3841 cgcgcagcgg caggcgcacc agaaaatgcc cgggaaatga caccccttcg agcggcagac
     3901 cgatgcgccg gccgagctcc atgtaaagga tggacatcgt gatcgggctg ccggtcttgc
     3961 gttcgatgac ctcgttcagg tagctgttgc gcggatcgta atagtcctcg gtattccccc
     4021 agtagccgag gtcgtcgtaa aggaactggt tcagcgccac gacgcgctcc tcggcg
//
LOCUS       DASTPD010000124        13998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1494488, whole genome shotgun sequence.
ACCESSION   DASTPD010000124 DASTPD010000000
VERSION     DASTPD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494488"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /gene="carB"
                     /locus_tag="VFR57_04385"
     CDS             complement(<1..1557)
                     /gene="carB"
                     /locus_tag="VFR57_04385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0258644.1"
                     /translation="MPKRTDIKSVLIIGAGPIVIGQACEFDYSGVQACKALRAEGYRV
                     VLVNSNPATIMTDPEMADATYVEPIQWQVVARIIEKERPDALLPTMGGQTALNCALDL
                     AREGVLEKYGVELIGASREAIDKAEDREKFKATMTKIGLGSARSALAHSMEEALQVQA
                     AIGYPVVIRPSFTLGGTGGGIAYNREEFIAICERGLEASPTKELLIEESLLGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQVMRDASIAVLRAIG
                     VDTGGSNVQFAVNPKDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IANDVTGGATPASFEPTIDYVVTKVPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEIGVDGLNQRTRDRETLEKELGEPGPERIWYVGDAFENGFTLDEVHRL
                     TRIDPWFLDQIREIVELEMKLEDQNLSQIDKDQLLELKRKGFSDRRLAYLFNTTEQEV
                     RAKRHKLEIRP"
     gene            complement(1563..2699)
                     /gene="carA"
                     /locus_tag="VFR57_04390"
     CDS             complement(1563..2699)
                     /gene="carA"
                     /locus_tag="VFR57_04390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU0258645.1"
                     /translation="MANLEPAVLVLADGTAFRGRSIGAHGTAVGEVVFNTAMTGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGVNDEDDESRRTYAEGLVIRDLPRLHSSWRAQTDLSS
                     YLKQNNVVGIADIDTRKLTRILREKGAQNGCLMGGEPDVKTALEQAREFPGLAGMDLA
                     KVVTTDKAYEWAEGSWQLGKGYERFDNPRFHVVAYDYGVKRNILRMMVDRGARVTVVP
                     AQMPAREVLRMKPDGVLLANGPGDPEPCDYAIEAIGEILDATRVPVFGICLGHQLMGL
                     ASGAKTMKMKFGHHGANHPVKDIDTGRVVITSQNHGFAVDPESLPHTLRPTHVSLFDG
                     SLQGLARTDRPAFCFQGHPEASPGPHDIGYLFDRFAKLMDDARK"
     gene            complement(2763..3512)
                     /gene="dapB"
                     /locus_tag="VFR57_04395"
     CDS             complement(2763..3512)
                     /gene="dapB"
                     /locus_tag="VFR57_04395"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU0258646.1"
                     /translation="MKLAIAGAAGRMGRAIAEAASADPEFSIVCAFDAAAAGESVGKV
                     KIVSDPAAVAQADVVLDFTRPEGTLALLKHAKAMVIGTTGFSPEDRSRISEAAKRIPI
                     VLAANFAVGVNTVYKLAETAARILGDAYDVEIIEAHHRHKVDAPSGTALQLGHVVAKA
                     LGRDLEKNAVFGRKGDVGERKAKEIGFHALRGGDIVGEHTVIFAGSGERVEVTVRSQS
                     RATYANGALRAAKFLKGKSARLYSMADVLGL"
     gene            complement(3523..3867)
                     /locus_tag="VFR57_04400"
     CDS             complement(3523..3867)
                     /locus_tag="VFR57_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEU0258647.1"
                     /translation="MHRWPRLLLVLAVASCGFVPQIPGVKPYRIEIQQGNYISPEAIA
                     SLQPGMSKEQVRLALGTPLLTDIFHADRWDYVYWRDKPGAKREQRKLTVYFEDGKLTR
                     LDGDPGAAGATQ"
     gene            3889..4317
                     /gene="fur"
                     /locus_tag="VFR57_04405"
     CDS             3889..4317
                     /gene="fur"
                     /locus_tag="VFR57_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEU0258648.1"
                     /translation="MSTVQSLKDSGLKATLPRRKILELFESSKVRHLSAEDVHKTLLA
                     EGIDVGLATVYRVLTQFEQAGLLARQHFETGKAVFELNQGGHHDHLLCLQCGRVEEFY
                     DADIEKRQTAVARTRGFSLRGHSLALYADCTKKDCPYRPA"
     gene            complement(4336..5973)
                     /gene="recN"
                     /locus_tag="VFR57_04410"
     CDS             complement(4336..5973)
                     /gene="recN"
                     /locus_tag="VFR57_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0258649.1"
                     /translation="MLRALEAANFVIIERASLEFGAGFSVLTGETGAGKSILVDAIEL
                     LVGGRGDASLVREGAERAELSAEFDLADRSFSAWLEEAGLAGDPGIVLLRRTIDRAGR
                     SRCFINGHAATLAQLKSAGEHLIDIHGQHEHQSLLRSAAQRALIDSHAGAQDLVAQVG
                     DAYRCWQRLQALAEEAEKNFAQREAERCQIQDTVTELKRLAPREGEWTDISAQHTRLQ
                     HGASLLGGAQSALEALEEGDGATLAQLSAVSSRLGALSAHDDHLKPVVELLESAEAQA
                     HEALRALRQYASRVELDPEALREVEARIEALHAAARKHRVRPENLPQLLADQEKRLHE
                     LELAVNEEALRREVASAQAQYINLAKHLSAKRRAAAEALGKAVSAAMQQLAMPGGRFG
                     VKLSATEEPAPSGLEEIEFEVASHPSLPLRPLAKVASGGELSRVSLAIQLVAAKASPI
                     GTLVFDEVDTGIGGAVAETIGRALRKLGKDRQVLCVTHLPQVAASGNDQWSVSRSGTK
                     VKVARLDRAARIAELARMLGGTETTARKHATGLLDAS"
     gene            complement(5975..6838)
                     /locus_tag="VFR57_04415"
     CDS             complement(5975..6838)
                     /locus_tag="VFR57_04415"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU0258650.1"
                     /translation="MEIIVHSSVMPSAFRRIALYGKLRSAETEASSTELREFLKKRGC
                     EVLPEGDTKADLAIVIGGDGTMLTAARELVRHQVPLVGINQGRVGFMTDIGHDDMQAG
                     IGAILDGKYSIEERTLLDAELVRDGKAALQTLALNEAVVGKGAQGRLIEFDLRIDGEF
                     IYTLRADGMIVATPTGSTAYALSAQGPILHPAVAALTLVPLNPHTLSARPVSVSDRSV
                     IEITLLRALDARAHFDGLALADMSEGDRLVLRRSADMVRFVHPPGYRYFATLREKLRW
                     SEKPLERRPAG"
     gene            6837..7853
                     /gene="hrcA"
                     /locus_tag="VFR57_04420"
     CDS             6837..7853
                     /gene="hrcA"
                     /locus_tag="VFR57_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0258651.1"
                     /translation="MLDKRAQILLKTLIERYIAEGQPIGSRTLSKYSGLDLSPATIRN
                     VMADLEELGFIASPHTSAGRVPTPLGYRFFVDTLLIVKPLESDAMHRLGLELLPDNPQ
                     RVVQAASQLLSQLTQFAGVVMTPRRRAVTFRHIEFLKLSEKRILLIVVTPEGDVQNRI
                     LFTERDYSPSELIEAANYINQNYSGHSFEDVRARLQGELRELRQDLTKLMSTALDAGS
                     RALAEGGDYVLSGERNLLTVQDLTHDVNRMKQLLELFERKSSLLSILDHSLRGQGVQI
                     FIGGESGVAAPDDVAVVTKPYTVDGEVLGTVGVIGPTRMAYDRVIPIVDVTAKLLSSA
                     LSQH"
     gene            7859..8722
                     /gene="hemH"
                     /locus_tag="VFR57_04425"
     CDS             7859..8722
                     /gene="hemH"
                     /locus_tag="VFR57_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU0258652.1"
                     /translation="MLLVNLGTPAAPSADAVRAYLAEFLADPRVVKLPRWLWLPILHG
                     IVLRTRPAKSAEKYAQIWMQEGSPLAVYTERQARLLGEKTGKPVRYAMRYGAPSIPEA
                     LKHLESPVVLPLYPQYAESTTASVADILPPGVRMIEHFHDHPAYIAALAANVARHREG
                     GMLVMSFHGLPKRGAERYEHECRETATLLARHLNIQRYIVTFQSRFGYAEWLGPATDA
                     TLRKLGAEGVERVEVVCPGFVADCLETLEEIGIAGRQAFLAAGGRAFRLLPCLNDSAE
                     WISAMAEIVRA"
     gene            complement(8748..9023)
                     /locus_tag="VFR57_04430"
     CDS             complement(8748..9023)
                     /locus_tag="VFR57_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258653.1"
                     /translation="MSNAERQELLRSAMVFISLRELSRRLRVSPSTLDEWISGEAPMP
                     DGAMFVLVAMLNEITWREQTTDAVKVEWSLGNPTTSKGRKNQGTDSR"
     gene            complement(9302..9475)
                     /locus_tag="VFR57_04435"
     CDS             complement(9302..9475)
                     /locus_tag="VFR57_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258654.1"
                     /translation="MKKRQEESWNEEDFGEDFQGDTKLFARLVEIDEIDDDELADQEE
                     VRRFANVARAWDA"
     gene            complement(9694..10035)
                     /locus_tag="VFR57_04440"
     CDS             complement(9694..10035)
                     /locus_tag="VFR57_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEU0258655.1"
                     /translation="MSWLITKYLVTAAVVVVVSEAAKLSDKLGGFIAALPLVTVLTLI
                     WLHIEDQPPAKVANHAWYTFWYVLPTLPMFLVFPALLSNIGFWPTLVTCLVITVVCFG
                     LLTLVGPPGLR"
     gene            complement(10068..12242)
                     /locus_tag="VFR57_04445"
     CDS             complement(10068..12242)
                     /locus_tag="VFR57_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEU0258656.1"
                     /translation="MEQGAHEHHHNHKHAHHAPPKPGAQYSCPMHPQIVRDAPGSCPI
                     CGMALVPIAGTGESDDSELRDLTRRFWIGAVLTIPLVLLAMAPMIGVMEPFGLAPRMR
                     GWVEFVLGTPVVGWVGWPIMKKFWLSLAHRALNMYTLIGLGVGLAYLFSLVAIFLPGL
                     FPQEFREHDGAVGTYFEAAAVIVTLVILGDVLQLRAMGRTSQAVRDLLKLAPNLAWRL
                     RADGTEEQVKLDDVKVGDRLRVKPGEKVPVDGTVLEGSSRIDESMVTGEPVPVAKQPG
                     DRVTGATINGSGSLVFRAERVGSDTLLARIVHMVGEAQRTRAPIQRLADLIAAYFVQI
                     VVAIAAVTALIWGFFGPEPRLTYALVNAVAVLIIACPCAVGLATPISITVAMGQGALS
                     GILFRNAEAIERMRDIDTLVVDKTGTLTLGRPALTNFLCDGILEREALALVAAAEQPS
                     EHPIATAIVEGAKARGLVMPPARDFDAVNGLGVQANVAGKRVLVGSRAYLAQSGVDTS
                     RWERQSDAWRAQAKTVVFFAVDGKAHGMAAVADPIKETTAEAIAKLKKERVRIVMLTG
                     DGKRTAEAVARQLGIDEAIGEVLPQDKAEHVRRLQAEGRKVAMAGDGINDAPALAQAD
                     VGIAMGTGTDIAMESAGVTLVKGDLRGIARAIRLSHATMRNIRQNLVFAFGYNALGIP
                     VAAGVLYPPFGLLLSPVFAGAAMALSSVSVVMNALRLRRTSL"
     gene            complement(12246..12449)
                     /locus_tag="VFR57_04450"
     CDS             complement(12246..12449)
                     /locus_tag="VFR57_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU0258657.1"
                     /translation="MLELDVTGMTCSHCVRAVTQAVRTVDPEAEVQIDLAAKRVRIQG
                     RSKADELIRVLRAAGYTARSAGG"
     gene            complement(12585..13451)
                     /locus_tag="VFR57_04455"
     CDS             complement(12585..13451)
                     /locus_tag="VFR57_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU0258658.1"
                     /translation="MLSLLLAALASATFAQSVTLHHVHGLAYSGDGKRLMIPSHHGIA
                     IYQDGKWSKAPGPEHDYMGFVATAKNLYSSGHPAPRSGFKEPFGLIRSRDGGKTWDKL
                     GLEGETDFHLMAAGWNTNAIYVWNPEPSSRMKTTGLHSTFNDGFTWQRVGAAGLGGKI
                     HALAAHPDDRNMVAIATSEGLYLSRNGGERFDKVAGNGQGLAAFFDLDGQHIWYGAYG
                     SRPTLSRLALKEPVPLSVPLPSLTNDAVSYIAQNPANRADYAIATFERSVYLSKDGGQ
                     SWQQIAHRGTAK"
     gene            complement(13474..13815)
                     /locus_tag="VFR57_04460"
     CDS             complement(13474..13815)
                     /locus_tag="VFR57_04460"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEU0258659.1"
                     /translation="MKHVLAVLVAGALAIGMVPMAAAQMQHGDMKGKDHGQPAKSQIH
                     KGAGVVTNVERDAGKVTLKHEPIESLNWPGMTMAFTVKDKALLDNLAKDKKVQFEFIQ
                     EGKQYVITSIK"
BASE COUNT         2325 a         4632 c         4455 g         2586 t
ORIGIN      
        1 gggccgaatt tccagtttgt ggcgtttcgc gcggacttcc tgctcggtcg tgttgaatag
       61 gtaggcaagg cgccggtccg agaatccctt gcgcttgagc tccagaagct ggtctttgtc
      121 gatctgggaa aggttctggt cttcaagctt catctcgagc tcgacgatct cgcggatctg
      181 gtccaagaac cagggatcga tccgcgtcag gcgatgcact tcgtcgagcg tgaagccgtt
      241 ctcgaaggcg tcgcccacat accagatgcg ctcggggccg ggctcgccga gctccttctc
      301 gagcgtctcg cggtcgcgcg tgcgctggtt caggccgtcg acgccgatct caaggccgcg
      361 cagcgccttc tggaacgact cctggaaggt gcggccgatc gccatcacct cgccgaccga
      421 cttcatctgc gtggtgagcc ggtcgttcgc ctgcgggaat ttctcgaatg cgaagcgcgg
      481 caccttggtg acgacgtagt cgatggtcgg ctcgaacgac gcgggcgtcg caccgccggt
      541 cacgtcgttc gcgatctcgt cgagcgtgta gcccaccgcg agcttcgccg cgaccttggc
      601 gatcgggaag cccgtggctt tggaggcgag cgcggaagag cgcgagacgc gcgggttcat
      661 ctcgatcacc accatgcgcc cgtccttcgg gttgacggcg aactgcacgt tcgatccgcc
      721 ggtgtccacg ccgatcgcgc gcagcaccgc gatcgaggcg tcgcgcatca cctggtattc
      781 cttgtcggtg agcgtctgcg ccggcgcgac ggtgatcgag tcgccggtgt gcacccccat
      841 ggggtcgagg ttctcgatgg aacagacgat gatgcagttg tccttgcggt cgcgcaccac
      901 ctccatctcg aactctttcc agccgaggag cgattcctcg atgagaagct ccttggtggg
      961 cgacgcctcg agcccccgct cgcagatcgc gatgaattcc tcgcggttgt aggcgatgcc
     1021 gccgcccgtg ccgccgaggg tgaaggacgg gcggatgacc accggatagc cgatcgccgc
     1081 ctggacctgg agcgcttcct ccatcgagtg cgcgagcgcc gagcgcgcgg agccgaggcc
     1141 gatcttcgtc atcgtggcct tgaacttctc gcggtcctcg gccttgtcga tcgcttcgcg
     1201 cgaggcgccg atcagctcga cgccgtattt ctccagcacg ccctcacgtg cgagatccag
     1261 tgcgcagttg agcgccgtct gcccgcccat tgtcggaagc agcgcatcgg ggcgctcctt
     1321 ctcgatgatg cgcgccacca cctgccactg gatcggctcg acgtacgtcg cgtcggccat
     1381 ttccgggtcg gtcatgatcg tggcggggtt cgaattcacc agcaccacgc ggtagccctc
     1441 ggcgcgcagc gccttgcagg cctgcacgcc ggagtaatcg aactcgcacg cctggccgat
     1501 gacgatcggg ccggcgccga tgatcaggac ggacttaatg tcagtgcgtt taggcaagct
     1561 ttctactttc gagcatcgtc catcagcttg gcgaaccggt cgaagagata gccgatgtcg
     1621 tgcgggccgg ggctcgcttc cgggtgtcct tggaaacaga acgccggtcg atcggtacgc
     1681 gcaagaccct gaagggaacc gtcgaagagg gaaacgtgcg ttggccgcag ggtgtgcggc
     1741 agcgactcgg ggtccaccgc gaagccatgg ttctggctcg tgatgaccac acgcccggta
     1801 tcgatgtcct tcaccgggtg atttgcgcca tggtgaccga acttcatttt catggtctta
     1861 gcgcctgagg cgagccccat gagttggtgg ccgaggcaga tgccgaacac cggtacgcga
     1921 gtggcgtcga ggatctcgcc gatcgcctcg atggcgtagt cacaaggctc cggatcgccg
     1981 gggccgttgg ccaggaggac tccgtccggc ttcatgcgca gcacttcgcg cgccggcatc
     2041 tgcgccggga cgacggtcac gcgcgcgccg cggtctacca tcatgcgcag gatgttgcgc
     2101 ttcacaccgt agtcgtaggc cacgacgtgg aagcgagggt tatcgaagcg ttcgtaaccc
     2161 ttcccaagct gccaggaacc ttcggcccat tcgtaggcct tgtccgtggt cacaaccttc
     2221 gcgaggtcca ttccggcgag gcccgggaat tcacgcgctt gctctaacgc cgtcttcaca
     2281 tcgggctcgc cgcccatgag gcaaccgttc tgcgcgccct tttctctgag gatgcgggtg
     2341 agcttgcgcg tgtcgatgtc ggcgatgcca accacgttgt tctgcttgag gtaagaagaa
     2401 agatcggtct gcgcgcgcca gctcgaatgc agccgcggca gatcgcggat cacgaggccc
     2461 tcggcgtaag tgcggcgcga ttcgtcgtcc tcgtcgttca cgccggtgtt gccgatgtgc
     2521 ggatacgtca gcgtgacgat ctggcgacag taagaggggt cggtgaggat ctcctgatag
     2581 ccggtcatcg ccgtgttgaa aacgacctcg cccacggccg tgccatgggc gccgatcgag
     2641 cggccgcgaa aggccgtccc gtcggcgagg accagcacgg cgggttcgag gttagccatt
     2701 tttgatgcgc atgcgaaaag cgggagtggc tctcggcccc tcccgcttca ttgaggatga
     2761 aattataggc ctaaaacgtc ggccatgctg tagagacggg cgctttttcc cttcaggaac
     2821 ttcgcggcgc gcagcgcgcc gttcgcgtag gtggcgcgac tctgcgagcg caccgtgact
     2881 tcgacgcgct cgccgctccc cgcgaagatc acggtgtgct cgccgacgat gtcgcccccg
     2941 cgcagcgcgt gaaagccgat ctccttcgcc ttgcgctcgc ccacatcgcc cttgcggccg
     3001 aaaacggcat tcttttcaag gtcccggcca agcgccttgg ctacgacgtg gcccagctgc
     3061 agcgcggttc ccgacggggc atccaccttg tggcggtggt gcgcctcgat gatctcgacg
     3121 tcgtaggcat cgccgaggat gcgcgcggcc gtctcggcga gcttgtacac cgtattcacg
     3181 ccgacggcga agttggctgc gagcacgatg ggaatgcgct tcgccgcttc cgaaatgcgg
     3241 ctcctgtctt ccgggctaaa gccggtggtg ccgatcacca tcgctttggc gtgcttgagg
     3301 agcgcgagcg tgccctcggg ccgcgtgaag tcgagcacca cgtccgcctg cgcgactgca
     3361 gctggatccg aaacgatctt caccttgccc acgctctcgc cggcagcggc cgcgtcgaac
     3421 gcgcacacga tggaaaactc gggatcggcg gatgcggctt ccgcgatcgc gcggcccatg
     3481 cgccccgccg cgccggcaat ggcaagcttc acgtggcggt tatcactgcg tggcgccggc
     3541 ggcgcctgga tcgccatcga ggcgcgtgag cttgccgtct tcgaagtaga cggtgagctt
     3601 gcgctgctcg cgcttcgcac cgggcttatc gcgccagtac acgtaatccc agcggtcggc
     3661 gtggaagatg tcggtgagaa gcggcgtgcc gagcgccagg cgtacctgct ccttgctcat
     3721 gccgggctgc aggcttgcga tcgcttccgg agaaatgtaa ttgccctgct ggatctcgat
     3781 gcgatagggc ttcacgccgg gaatctgcgg cacgaagccg cagctcgcga cggcgagaac
     3841 gaggagcagc cggggccagc gatgcatggc cgcatataat gacagccgat gagcacggtg
     3901 caaagcctta aggacagcgg gctcaaggcg acactgcccc ggcgaaagat cctcgagctc
     3961 ttcgagtcga gcaaggtccg gcatctctcc gcggaggatg tccacaagac gctcctcgcc
     4021 gagggcatcg atgtgggtct cgcgacggtc taccgcgtcc tcacgcagtt cgagcaggcc
     4081 ggcttgcttg cgcgtcagca cttcgagacc ggcaaggcgg tattcgagct gaaccagggc
     4141 gggcaccatg accacctcct ctgcctgcaa tgcggccgcg tcgaggagtt ctacgacgcc
     4201 gacatcgaaa aacgccagac cgccgtcgcg cgcacgcgcg gcttctcgct ccgcggccac
     4261 tccctcgcgc tctacgccga ctgcacgaag aaggactgcc cttaccgccc cgcgtaatcg
     4321 ggctctgacc ccgatttacg acgcgtctag aagccccgtg gcgtgcttgc gcgccgtggt
     4381 ttcggtgccg cccagcatgc gggcgagctc ggcgatgcgc gcggcgcgat cgaggcgcgc
     4441 gactttcacc ttggtaccgg agcgcgacac ggaccactga tcgttgccgc tcgccgcgac
     4501 ctgcggaagg tgcgtgacgc agagcacctg gcggtccttg ccgagcttgc gtagcgcgcg
     4561 gccgatggtt tcggcgacgg caccgccgat gccggtgtcg acctcgtcga agacgagggt
     4621 accgatcggc gaggccttgg ccgcgaccag ctggatggcc aggctgacgc gcgagagctc
     4681 tccaccggac gccaccttgg cgagcgggcg aagcggcagg ctcggatgcg aggcgacttc
     4741 gaactcgatc tcctccagcc cgctcggcgc gggctcttca gtagcgctca gcttcacgcc
     4801 gaagcggccg cccggcatgg cgagctgctg cattgccgcg ctcaccgcct taccgagcgc
     4861 ctcggccgcc gcacgccgtt tcgccgagag atgcttggcg agattgatgt actgcgcctg
     4921 cgcgctcgcc acttcgcggc gcagggcttc ctcgttcacg gcgagctcga gctcgtggag
     4981 ccgcttttcc tgatcggcca aaagctgcgg caggttttcg gggcgcacgc ggtgcttgcg
     5041 cgcggcggcg tgcagcgcct cgatgcgcgc ctcgacttcg cgcagcgcct ccgggtccag
     5101 ctcgactcgc gaagcatact ggcgcaaggc acgcagcgct tcgtgcgcct gcgcttcggc
     5161 cgattccaaa agctcgacca ccggtttcag gtggtcgtcg tgcgccgaga gcgctcctaa
     5221 gcggctggag acggcggaga gctgcgcaag tgtcgcgccg tcgccttcct cgagcgcctc
     5281 gagcgcggac tgcgcgccgc cgagcaggct cgccccgtgc tggagccgcg tgtgctgcgc
     5341 ggaaatgtcg gtccactcgc cttcgcgcgg cgccaggcgc ttgagctccg tcacggtgtc
     5401 ctgaatctgg cagcgctcgg cttcacgctg cgcgaaattt ttctctgcct cctcggcgag
     5461 cgcctgcagc cgctgccagc acctgtacgc gtcgccgacc tgcgcgacca ggtcttgcgc
     5521 accggcatgc gaatcgatga gtgcgcgctg cgccgcggag cgaagcaacg actggtgctc
     5581 gtgctggcca tggatatcaa tgaggtgctc gcccgcgctt ttgagctgag cgagcgtggc
     5641 ggcgtgaccg ttgatgaagc agcgcgagcg ccccgcccga tcgatcgtgc gccgcaggag
     5701 aacgatgcct ggatccccgg ccaggcccgc ttcttcgagc caggccgaga aagaacgatc
     5761 cgccaggtcg aactcggccg agagctcggc gcgctcggcg ccttcgcgca ccagggaggc
     5821 gtcgccgcgc ccgcccacga ggagctcgat cgcgtcgacg aggatcgact tgccggcgcc
     5881 ggtctcgccg gtgagcacgc tgaagcccgc gccgaactcg agcgacgctc gttcgatgat
     5941 gacgaagttc gccgcctcga gcgcgcgcag catgctatcc cgccggccgg cgttcgagcg
     6001 gcttctcgct ccagcgcagt ttctcgcgca gcgtcgcgaa gtaacggtag cccggcgggt
     6061 gcacgaaacg caccatgtcc gcggaacgcc tgaggacaag gcggtcgccc tcgctcatgt
     6121 cggcgagcgc cagtccatcg aaatgcgcgc gcgcgtcgag cgcgcgcagc agcgtgatct
     6181 cgatgaccga acggtcgctc acgctcaccg gccgcgcgga cagcgtgtgc gggttgagcg
     6241 gaacgagcgt aagcgccgcg accgcaggat gcaggatcgg accctgcgcc gagagcgcat
     6301 acgcggtcga tcccgtcggc gtagcgacga tcatgccgtc ggcgcgcaac gtgtagatga
     6361 actcgccgtc gatgcgcaga tcgaactcga tcaggcgccc ttgcgcgccc ttgccgacca
     6421 ccgcttcgtt gagggcgagc gtctgcagcg ccgctttccc gtctcgcacc agctcggcgt
     6481 cgagcagcgt gcgctcctcg atcgagtact tgccatcgag gatcgcgccg atgccggcct
     6541 gcatatcgtc gtgcccgatg tcggtcatga agccgacgcg gccttggttg atgccgacga
     6601 gcggaacttg atggcgcacg agctcgcgcg cagcggtgag catcgtgccg tcgccgccga
     6661 tcacgatggc aaggtcggcc ttggtgtcgc cttcggggag aacttcgcag ccgcgcttct
     6721 tcaggaattc gcgcagctcg gttgacgaag cttcggtctc cgcgctgcgc agcttcccgt
     6781 agagggcaat cctgcggaac gcggagggca tgacgctcga gtgtacaatg atttccatgc
     6841 tcgacaagcg cgcgcaaatc ctgctcaaga ccttgatcga gcgctacatc gccgaaggcc
     6901 agccgatcgg ctcgcgcacg ctttcgaagt attccgggct cgacctttcg cccgcgacga
     6961 tccgcaacgt gatggccgat ctggaagagc tcggcttcat cgcgagcccg cacacctcgg
     7021 ccggccgcgt tccgacgccg ctcggctacc gcttcttcgt cgacacgctg ctcatcgtga
     7081 agccgctcga gtccgacgcc atgcatcgcc tcgggctcga gctgctgccg gacaatccgc
     7141 agcgcgtggt gcaggccgcc tcgcagctcc tgtcgcagct cacgcaattt gccggcgtag
     7201 tcatgacgcc gcgccggcgc gccgtcactt ttcgccacat cgagtttctc aagctttcgg
     7261 agaagcgcat cctgctcatc gtcgtcacgc cggagggcga cgtgcagaat cggatcctct
     7321 ttaccgagcg cgattactcg ccctcggagc tcatcgaggc cgcgaactac atcaaccaga
     7381 actactccgg gcacagcttc gaggatgtgc gcgcgcgcct gcagggcgaa ctgcgcgagc
     7441 tgcgccagga tctcaccaag ctcatgtcga ccgcgctcga cgcgggcagc cgggcgctcg
     7501 cggagggcgg cgactacgtg ctttcaggcg agcgcaacct gctcacggtg caggacctca
     7561 cgcacgacgt gaaccggatg aagcagcttc tcgagctttt cgaacgcaag agctcgcttc
     7621 tctcgatcct ggatcactcg ctgcggggcc agggcgtgca gatcttcatc ggcggcgaat
     7681 ccggcgtcgc cgcgcccgac gacgtcgcgg tggtcaccaa gccctacacc gtcgacggcg
     7741 aggtgctcgg cacggtcggc gtgatcggcc cgacgcgcat ggcgtacgac cgggtgattc
     7801 cgatcgtcga cgtcacggcg aagctcctct cgagcgcgct ctcgcagcac tgagcagcat
     7861 cctcctcgtc aacctgggca cgccggccgc gccatcggcc gatgccgtgc gcgcctatct
     7921 cgccgagttc ctcgccgatc cgcgcgtcgt caagctgccg cgctggctgt ggcttccgat
     7981 cctccacggc atcgtgctgc gcacccgccc cgcaaagtca gcggaaaagt acgcgcagat
     8041 ttggatgcaa gaaggctccc cgctcgccgt gtatacggag cggcaggccc gactcctcgg
     8101 agagaaaacc ggcaagccgg tgcgctacgc gatgcgctac ggcgcgccct ccatccccga
     8161 ggctttgaag cacctggaaa gccccgtggt gctgccgctt tatccgcagt acgccgaaag
     8221 cacgacggcg agcgtcgccg acatccttcc gcctggcgtg cgcatgatcg agcacttcca
     8281 cgaccacccc gcgtacatcg ccgcgctcgc cgctaatgtg gcgcggcatc gcgaaggcgg
     8341 catgctcgtc atgagcttcc acggcctgcc gaaaaggggc gcggagcgct acgagcatga
     8401 atgccgcgaa acggcgacgc ttctcgcgcg ccacctgaac atccaacgtt acatcgtgac
     8461 tttccagtcg cgcttcggtt acgcggagtg gcttggccct gcgaccgacg ccacgctgcg
     8521 caagctcggc gccgaaggcg tggagcgcgt ggaggtcgtc tgccccggat tcgttgccga
     8581 ctgcctcgag acgctcgagg aaatcggcat cgccggacgg caggctttcc tcgccgcagg
     8641 cgggcgcgcg ttccgcctgc tcccgtgtct caacgactcc gcggagtgga tcagcgccat
     8701 ggcggagatc gtccgcgcat gactcgccgt cctgggctcg gctagattca tcggctatcg
     8761 gttccttgat ttttgcggcc tttggaagtc gtcgggttac ctaacgacca ttcgaccttc
     8821 accgcatcgg tggtttgctc gcgccaagtg atttcgttga gcatggcaac cagcacgaac
     8881 atcgcgccat ccggcatcgg cgcttccccg gaaatccatt cgtccagcgt ggacggtgaa
     8941 actcgcaggc gtcgcgaaag ctcgcgcagc gatatgaaaa ccattgcgct gcggagcagc
     9001 tcttgccttt ctgcgttcga cacagcttca atctaccggc gctcgttccg aggcggtatc
     9061 ccatatcgcc ctacacgatg agcaagatca aacgttggat cgtcgatcgc tcgcggcttg
     9121 ctttatctca ctgtccgcgc gcttggccga tttttttgtc tttgaggccg aagaggatcc
     9181 gggcgggcgc ggcgtgtgga ggcggctatc gccattctct ccacttgtgc gacgccgctg
     9241 cccgcccggt tgctcgattt cttaggaccg cgccgaggcc gccggtatag ctagctagga
     9301 attacgcgtc ccacgcgcgc gcaacattcg caaagcgcct cacctcttcc tgatcggcaa
     9361 gctcatcgtc gtcaatctcg tcaatctcga cgaggcgagc gaaaagcttc gtatcgcctt
     9421 ggaagtcttc gccgaagtct tcttcgttcc agctttcttc ttgccgcttc ttcatgcccg
     9481 gtctccccct aaagtcctca actctgaccg catcggattc ccatttcgtc ttcttaacgg
     9541 tcagtggatt tcatacagga aggtaaaggg atggttgcct tgatgcatga gatgacccgc
     9601 acgggctaac atgcgaaaag caactaggtg cttagtatca acgtaccaaa attgcgctcg
     9661 ggtcgcgctc gtacctatgc agcgtcccgc cactcatctg agccctggtg ggcccacgag
     9721 cgtcaagagt ccgaagcaga ccactgtaat cacgaggcat gtcaccagcg tgggccagaa
     9781 gccgatgttg gacagcaagg ccgggaatac gaggaacatc ggcagcgtcg gcagcacgta
     9841 ccagaacgta taccaagcgt ggttcgcgac cttggcgggt ggctggtcct cgatatgcag
     9901 ccagatcagg gtcaagactg ttaccaatgg aagcgcggcg atgaatccgc ccagcttgtc
     9961 gctgagcttt gccgcttcgg agacgaccac taccacggct gcagtgacga gatacttggt
    10021 gatcagccac gacatccgac attccctttc gcagatttac ttcaatctca aagcgatgtc
    10081 cgacgcagcc gcagcgcatt catgaccacc gacacggagc taagtgccat ggccgcgccg
    10141 gcgaataccg gagacagcag cagcccgaac ggcggataga gcacgccggc cgcaaccggt
    10201 atgccgagcg cgttgtagcc aaaggcgaac acaaggttct ggcggatatt gcgcatcgtg
    10261 gcgtgcgaca gacggatcgc acgcgcgatg ccgcgcaggt cgcctttcac aagggtgacg
    10321 cccgcgcttt ccatcgcgat gtcggttccg gtccccatcg cgatgccgac gtcggcctgc
    10381 gcaagcgccg gcgcgtcgtt aatgccatcg cccgccatgg cgaccttgcg cccctcagcc
    10441 tgcaggcgcc tgacgtgctc cgccttgtct tgcggcagga cttcgccgat cgcctcgtcg
    10501 atgccgagct ggcgcgcaac cgcctcggcg gtccgcttac cgtcaccggt gagcatgaca
    10561 atgcgtacgc gctctttctt cagcttcgcg atcgcttcgg ccgtcgtttc cttgatcggg
    10621 tctgcgaccg ccgccatgcc gtgcgccttg ccgtcgactg cgaagaacac caccgtcttc
    10681 gcttgggcac gccatgcgtc cgactggcgt tcccagcgac tcgtgtcgac gccggattgg
    10741 gccaggtacg cgcggctacc cacaaggacc ctcttgccgg caacgttcgc ctgcactccc
    10801 aggccgttca cagcgtcgaa gtcgcgcgcg ggcggcatca cgagcccgcg cgctttggca
    10861 ccttcgacga tcgccgtcgc gatggggtgt tcggagggct gttcggcggc ggcgacgagc
    10921 gcaagggcct ccctttccag aatgccgtcg cacagaaagt tagtgagcgc aggccggccg
    10981 agcgtcagcg tgccggtttt gtccacgacc agcgtatcga tgtcgcgcat acgctcgatc
    11041 gcctctgcat tgcgaaacag gatccccgaa agcgcgccct gccccattgc cacagtgatc
    11101 gagatcggag tagccaggcc caccgcgcac gggcaggcga tgatgagcac ggccaccgcg
    11161 ttgacgagcg cgtacgtgag gcgcggctcc ggtccgaaaa aaccccatat caacgccgtg
    11221 accgcagcga tggcgacaac gatctgcacg aagtaggcgg cgatcagatc ggcgagccgc
    11281 tggatcggcg cgcgggtgcg ctgtgcttcg ccgaccatgt ggacgatgcg cgcaagcagc
    11341 gtgtctgagc cgacgcgctc ggcacggaac acgagagatc cggagccgtt gatggtcgct
    11401 ccggttacgc ggtcacccgg ttgctttgcc acgggaaccg gttccccggt caccatggat
    11461 tcgtcgatgc gacttgagcc ttcgaggact gtgccgtcca cgggcacctt ctcgccgggc
    11521 ttcacgcgca gccgatcacc caccttcacg tcatcgagct tcacctgctc ttccgtcccg
    11581 tcggcacgca ggcgccacgc caggttcggt gcgagcttca ggagatcgcg caccgcctgg
    11641 ctcgttcgac ccatcgcacg aagctgcagc acatcgccga ggatgaccag cgtgacgatg
    11701 accgccgccg cttcgaagta cgttcccacg gcgccgtcgt gctcccgaaa ctcctgcgga
    11761 aagaggcccg gcagaaagat cgcgaccagg ctgaacaggt aggcgagccc gacccccagg
    11821 ccgatgagcg tgtacatgtt gagcgcgcgg tgcgcgagcg agagccagaa cttcttcatg
    11881 atcggccagc ccacccatcc caccacgggc gtgccgagca caaattcaac ccagccgcgc
    11941 atgcgcggcg caaggccgaa cggttccatg acgccgatca tcggcgccat ggcgagcagc
    12001 acgagcggga tggtgagaac agcgccgatc cagaatcggc gcgtaagatc gcgtagctcg
    12061 ctgtcatcgg actcgccggt gcccgcgatt ggcacgagcg ccatgccgca gatcggacag
    12121 cttcccggcg catcgcgcac gatctgcgga tgcatcggac agctgtactg agcgccgggc
    12181 ttcggaggcg cgtggtgcgc atgcttgtgg ttgtgatgat gctcgtgcgc gccctgctcc
    12241 atggtctagc cgccggcgct acgcgccgta taacccgcag cgcggagcac gcggatcagc
    12301 tcatcggcct tgctgcgccc ttgaatgcgc acgcgcttcg ctgccagatc gatctgaacc
    12361 tctgcttcgg gatccaccgt tctcacggcc tgcgttacgg cacgtacgca atgactgcag
    12421 gtcatacctg tcacatcgag ctccagcatt gccgtctcct aagaggttat tcactgccgc
    12481 cagcttggac gttcccatgg tggcagggtc aagcgcgtca tgcgccatag attagagtgg
    12541 atcaggccgc caagcttggc ggtcacggaa cgagagcgcc tcgctcactt cgccgtgccg
    12601 cgatgggcga tctgctgcca gctttgtccg ccgtccttag agaggtagac gctgcgctcg
    12661 aaggtcgcga tcgcgtagtc ggcgcggttc gcggggtttt gcgcgatata actgaccgcg
    12721 tcgttcgtga gtgacggcag cggcacgctg agcggcaccg gctccttgag cgccaggcgc
    12781 gaaagcgtcg gtcggctgcc ataagcgccg taccagatgt gctgtccatc aagatcgaag
    12841 aacgcggcga gcccctgccc gttgccggcg accttatcga agcgttcgcc gccgttgcgg
    12901 gaaagataaa ggccttcgga agtggcaatc gcgaccatgt tgcgatcgtc gggatgcgct
    12961 gcgagcgcgt ggatcttgcc gccgaggccc gcggctccta ctcgctgcca ggtgaagccg
    13021 tcattgaacg tggagtgcag gccggtcgtc ttcatgcgcg agctcggctc ggggttccag
    13081 acgtagattg cattggtgtt ccagcccgct gccatgaggt gaaaatcggt ctcgccttcc
    13141 aggcccagct tgtcccaggt cttgccgccg tcgcggctgc ggatcagacc gaacggttct
    13201 ttgaagccgg agcgcggcgc tggatggccg ctgctgtaga ggttcttcgc tgtggcgacg
    13261 aatcccatgt aatcgtgctc cggtcctggc gccttggacc acttgccgtc ctggtaaatg
    13321 gcgatgccgt ggtggcttgg gatcatgagg cgcttgccgt cgccgctgta cgcgaggccg
    13381 tgcacgtggt gcagcgtgac tgactgggcg aaggtcgccg aggcgagcgc cgcgagcagt
    13441 aatgaaagca cggcacgtgg gatcatcggt cagtcacttg atggaggtaa tcacgtactg
    13501 cttgccttcc tgaatgaatt caaactgcac tttcttgtcc ttggcgagat tatcgagcag
    13561 cgccttgtcc ttcacggtga aagccatcgt catgccgggc cagttgaggc tctctattgg
    13621 ctcgtgcttg agcgtcacct tgccggcgtc gcgctcgacg ttggtcacca cgccggcacc
    13681 tttgtggatc tggctcttcg ccggctgccc gtgatccttc cccttcatgt cgccgtgctg
    13741 catctgcgcc gcggccatcg gcaccattcc gatcgccaac gcgccagcga ccaggaccgc
    13801 aagtacatgc ttcatgctcg ctctcctttg atgagttcca tatgcgttgc cagtttgccc
    13861 cgctatccgt cgttagatat acgtcacgcc ggcgcgttgc actcgcgtcc atcaagcgcc
    13921 gattcgatga gcgcgctcga gagcgcgcgg gcataccgca gccgctttcc ttggaggtcg
    13981 aaggccacgg cggtcacc
//
LOCUS       DASTPD010000125         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1289628, whole genome shotgun sequence.
ACCESSION   DASTPD010000125 DASTPD010000000
VERSION     DASTPD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289628"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="yidC"
                     /locus_tag="VFR57_04465"
     CDS             complement(<1..1635)
                     /gene="yidC"
                     /locus_tag="VFR57_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0258660.1"
                     /translation="MDTQRLILLVIFSFSLLMLWEAWEKEKRPKPPPTAPTEQPGVPA
                     PAQPAPPPAAKPSPAVPGAEAAKGEVIRVSTDLVVAEIDTLGGTLKRVELVRHKDSKD
                     LSKNLVLLGSERQYEAQSGLAGEGGPNHRTLWRVEPGERALAPGRDEVAVQLAATGKD
                     GLEVRKSYTFKRSSYVVDVVLDVRNPGAAPVAAYAYFQLTHDGKTETNTTTVAETFGA
                     QSFIGFAVYTDERKFEKIHLSDLDKGKADYVKQATNGWLAFVQHYFVSAWLPPHGLQR
                     DYAAEKRPDGIYAARAMVPVSVAPSGSAAVNVPLYVGPQEQRRLQAAAPGLDLVVDYG
                     WLAIIAWPLFWLLEKFYALSGNWGIAIILLTVLIKLVFFPLSAASYKSMAKMKLITPR
                     LQKLREMYAHDRQKMNQAMMELYKTEKINPLGGCFPILVQIPVFIALYWVLLAAIELR
                     HAPFMLWIKDLSALDPYYVLPILMTATMILQTRMNPVPPDPVQARVMQIMPFVFSIFF
                     FFFPAGLVLYWLVNNILSIAQQWQIQRMFDRDKPAHA"
     gene            complement(1639..1851)
                     /gene="yidD"
                     /locus_tag="VFR57_04470"
     CDS             complement(1639..1851)
                     /gene="yidD"
                     /locus_tag="VFR57_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0258661.1"
                     /translation="MTVRALCFLLLAYRWCIRPLLPSACRFYPSCSCYAEEALLRHGF
                     WRGGWLAARRVCRCGTWHSGGHDPVP"
     gene            complement(1851..2171)
                     /gene="rnpA"
                     /locus_tag="VFR57_04475"
     CDS             complement(1851..2171)
                     /gene="rnpA"
                     /locus_tag="VFR57_04475"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU0258662.1"
                     /translation="MDAARRLRQKTDFERLLRHGARRNLDGYTMYVGRRDSGAARLGL
                     LISRRHAPRAVERNRIKRCIREAFRLEQERLGPIDVLVRPPYGVNPSAQMIVRLRELF
                     GRLI"
     gene            complement(2195..2335)
                     /gene="rpmH"
                     /locus_tag="VFR57_04480"
     CDS             complement(2195..2335)
                     /gene="rpmH"
                     /locus_tag="VFR57_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU0258663.1"
                     /translation="MATKRTYQPSKTRRARRHGFLVRSRTAGGRKVLRNRRARGRRRL
                     GL"
     gene            2454..3776
                     /gene="dnaA"
                     /locus_tag="VFR57_04485"
     CDS             2454..3776
                     /gene="dnaA"
                     /locus_tag="VFR57_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU0258664.1"
                     /translation="MHNLWDACLRRLEQELPAQQFNTWIRPLAPETAQAADTLVLAAP
                     NRFVLELVRERFAARIERLASEASGRDLGLRLVLARMAEPAAPQPVPRAAETPSILER
                     ARLNPAFSFDSFVTGKANQLARAAALQVAENPGVSYNPLFVYGGVGLGKTHLVQAIGN
                     QLADQRPQARIRYIHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSNKGR
                     TQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVAIEPPELEMRV
                     AIVLKKAEAESVALSEDIAFFIAKHLRSNVRELEGALKKVLAFARFSGRELSLELAKE
                     ALQDILNVASRQVSVEGIQKTVAEYFKIKISDMHSKKRSRNVARPRQVAMALAKDLTQ
                     MSLPEIGEAFGNRDHTTVLHACRTIASLRKNDTALNRDYVVLEQVLKG"
BASE COUNT          767 a         1280 c         1290 g          707 t
ORIGIN      
        1 ggcatgggca ggcttgtcgc ggtcgaacat gcgctggatc tgccattgct gggcgatgga
       61 gaggatgttg ttgaccagcc agtagagcac gaggccggcc gggaagaaaa agaagaagat
      121 gctgaagacg aacggcatga tctgcattac ccgagcctgc accggatcgg gcggcacggg
      181 attcatccgc gtctgcagta tcatggtcgc cgtcatcagg atgggcagga cgtagtatgg
      241 gtcgagcgcg gacaggtcct tgatccacag catgaacggc gcgtggcgta gctcgattgc
      301 cgcaagcagg acccagtaca gcgcgatgaa caccggaatc tgcaccagga tcgggaagca
      361 gcccccgaga gggttgatct tctcggtctt gtagagctcc atcatcgcct ggttcatctt
      421 ctgccggtcg tgcgcgtaca tctcgcgcag cttctgcagc cgcggcgtga tcagcttcat
      481 cttcgccatc gacttatagc tggcggcgga gaggggaaag aacacgagct tgataagcac
      541 cgtgagcagg atgatcgcga taccccagtt gcccgacagc gcgtaaaact tctccagaag
      601 ccagaacagc ggccaggcga tgatcgcaag ccagccgtaa tccactacca ggtcgaggcc
      661 cggtgcggcc gcttgcaggc ggcgctgctc ctgcggtccc acatagagcg gcacgttgac
      721 cgccgcgctt ccgctcggcg ccacgctcac cggcaccatc gcccgggcgg cgtaaatgcc
      781 atcggggcgc ttttccgctg cgtagtcgcg ctgcaagcca tgcggaggca gccaagcgga
      841 gacaaaataa tgctgcacaa acgcaagcca gccgttggtt gcctgcttaa cgtaatcggc
      901 cttgcccttg tcgaggtccg acagatggat cttctcgaac ttgcgctcgt cggtgtacac
      961 ggcgaagcca atgaagctct gcgcgccgaa agtctcggcg accgtagtcg tgttcgtttc
     1021 cgtcttgccg tcgtgcgtga gttggaagta cgcgtaggcg gccaccggcg cggcaccggg
     1081 attgcgcacg tccagcacca cgtcgacgac atagctgctc cgcttgaagg tgtaactctt
     1141 gcgcacttcg agcccgtcct tcccagtggc ggcgagctgt accgccacct cgtcgcggcc
     1201 tggggcgagc gcgcgctctc ccggctcgac gcgccacagc gtgcggtggt taggtccgcc
     1261 ttcgccggcg aggccgcttt gcgcttcgta ctgacgctcg gagcccagta ggacgaggtt
     1321 cttggaaagg tccttggagt ccttgtggcg cacaagctcc acacgcttca gcgtccctcc
     1381 gagcgtgtcg atctcggcca cgacgaggtc cgtgctcacg cgtatgacct cgccctttgc
     1441 cgcttcggcg ccgggaacag caggcgaggg tttggcggct ggcggcggcg ccggctgggc
     1501 cggcgcgggt acgcccggct gctccgtcgg cgcggtgggt ggcggcttcg ggcgcttctc
     1561 cttctcccat gcttcccaaa gcatgagaag cgagaagctg aagatcacga ggagaatcag
     1621 cctttgcgta tccatagcct acgggacggg atcgtgaccg ccggaatgcc aggtgccgca
     1681 gcggcatacg cgccgcgctg cgagccagcc gccgcgccag aacccgtgtc gcagcaacgc
     1741 ttcctctgcg tagcatgagc agctcgggta gaaacggcac gctgacggga gcaaaggccg
     1801 gatgcaccag cgataagcga gcagcaggaa gcacagcgcg cgcaccgtca ttagatgagc
     1861 ctgccaaaaa gctcgcgcag gcggacgatc atctgcgcgg aaggatttac accataaggc
     1921 gggcgcacca gcacatcaat agggccgagc cgctcctgct ccaggcggaa agcctcgcgg
     1981 atgcaacgct tgatgcgatt acgctcgacg gcgcgcggcg cgtgcctgcg cgagatcagg
     2041 aggccaagcc gcgccgctcc gctgtcgcgt cgccccacat acatggtgta gccgtccagg
     2101 ttgcgccgcg cgccgtgtcg cagcagccgt tcgaagtcgg tcttttgcct cagccggcgt
     2161 gcggcatcca gcccgaagcg cttgccggcc gcggctacag cccgagccgg cgccggccgc
     2221 gagcgcgccg gttacgcagc actttgcgac cgccggcagt ccggctgcgc acgagaaaac
     2281 catggcgccg cgcgcgccga gtcttggaag gttggtaagt ccgcttcgtc gccatctgcc
     2341 ggtccgaaaa agggcggtat tagagcgcgc gcgcgcgcgc attgtcaatt cgtctgtgga
     2401 tatggcatgc ctaagaaggt aaaattccga ttcgctttcg gcccaacgaa aagatgcata
     2461 acctctggga tgcctgcctg cggcgcctcg agcaggagct ccccgcgcaa cagttcaaca
     2521 cgtggatccg gccgcttgcg ccggaaacgg cccaggcggc cgacacgttg gtcctggcgg
     2581 caccgaatcg gttcgtgctg gagctggtgc gcgagcgctt cgccgcgcgc atcgagcgtc
     2641 tggcatccga ggcgagcggg cgcgacctag gcttgcgcct cgttctggcg cgcatggcgg
     2701 agcctgcggc gccgcaaccc gtcccgcgtg ccgccgagac gccgtcgatt cttgagcgcg
     2761 ccaggctcaa tccggcgttc tcgttcgaca gcttcgtcac cggaaaggcg aaccagcttg
     2821 cgcgcgccgc cgcgctccag gtcgcggaaa atcccggggt ttcctataac ccgcttttcg
     2881 tctatggcgg cgtgggactc ggcaagacgc acctggtgca ggcgatcggc aaccagctcg
     2941 ccgaccagcg gccgcaggcg cggatccgct acatccacgc cgagcagtac gtcaccgacg
     3001 tggtgcgggc ttaccagcag aagtcgttcg acgagttcaa gcgctattac cactcgctcg
     3061 accttctgct gatcgacgat atccagttct tctccaacaa gggacggact caggaagagt
     3121 tcttttacgc cttcaacgcg ctggtggagg cacacaagca gatcgtgatt acctgcgata
     3181 cctaccccaa ggagatcgcc ggcatggagg agcggctgat ctcgcgcttc ggctggggtc
     3241 ttaccgtagc gatcgagccg ccggagctcg agatgcgcgt ggcaatcgtg ctgaagaagg
     3301 ctgaggccga gtcggtcgcg ctgtccgagg acatcgcttt cttcatcgcc aagcacctgc
     3361 gctccaacgt gcgcgagctc gagggcgcgc tgaagaaagt gctcgcgttc gcgcgctttt
     3421 ccgggcgcga gctttcgctc gagctggcga aggaagccct gcaggacatc ctgaatgtcg
     3481 ctagccgcca ggtcagcgtc gaaggcatcc agaagaccgt cgcggagtat ttcaagatca
     3541 agatttccga catgcactcc aagaagcgca gccgcaacgt ggcgcgcccg cgccaggtcg
     3601 ccatggcgct cgcgaaggac ctcacccaga tgagcttgcc ggaaatcgga gaggcgttcg
     3661 gcaaccgcga ccataccacc gtgctgcacg cctgccgcac tattgccagc ctgcgcaaga
     3721 acgataccgc tctcaatcgc gactatgtgg tactcgaaca ggtgctgaaa ggctaaggaa
     3781 aagttggggg cggattgtgc gtaaccaagg cgatcgtcgc ttgagcgcga acgatccaca
     3841 ctggcgcgcg ccccgtccac agcttttaca gcgctcgaac ggacccgtaa aacgcgtctg
     3901 ggcaaggact tgcatactta tcccgcgatc gccctggctc tattagctat tactaaggta
     3961 aataacaatg aatcttgcaa aagcaaagcg cgacgagttc ctgggaccgc tttcggccgt
     4021 atgcgggatc atcgagcggc gcca
//
LOCUS       DASTPD010000126         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_882339, whole genome shotgun sequence.
ACCESSION   DASTPD010000126 DASTPD010000000
VERSION     DASTPD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882339"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="rpsM"
                     /locus_tag="VFR57_04490"
     CDS             <1..199
                     /gene="rpsM"
                     /locus_tag="VFR57_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0258665.1"
                     /translation="LTVEGDLRREVQMSIKRLIDLSCYRGSRHRKGLPVRGQRTRTNA
                     RTRKGPRKAAIKLNVPAGKAA"
     gene            203..628
                     /gene="rpsK"
                     /locus_tag="VFR57_04495"
     CDS             203..628
                     /gene="rpsK"
                     /locus_tag="VFR57_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU0258666.1"
                     /translation="MASRNQPTRQELQQTASARARKKVKKNVAEGIAHIHASFNNTIV
                     TITDRQGNTLAWATSGHAGFKGSRKSTPFAAQVAAERCGRAAQECGVKNIEVRIKGPG
                     PGRESAVRALNAIGLKIGSIADVTPIPHNGCRPPKRRRV"
     gene            635..1261
                     /gene="rpsD"
                     /locus_tag="VFR57_04500"
     CDS             635..1261
                     /gene="rpsD"
                     /locus_tag="VFR57_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0258667.1"
                     /translation="MARNLDKCRHCRREGEKLFLKGEKCFTDKCPVERRAYAPGQHGQ
                     KSGARLSDYGKQLREKQKVRHIYGLLERQFRKTYAEAARSKGVTGERLLQLLESRLDT
                     VVYRMGFGASRTEARQIVRHNGILVNGKRVNIPSYQVRAGDVIEVAGGAKEQLRIKAA
                     AEAAASRGIPEWMEVDSKGLKGTFKNLPARSDLPQTINESLIIELYSK"
     gene            1274..2263
                     /gene="rpoA"
                     /locus_tag="VFR57_04505"
     CDS             1274..2263
                     /gene="rpoA"
                     /locus_tag="VFR57_04505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0258668.1"
                     /translation="MPQSGFLKPRIVDVQNLSPNHARVTMEPFERGYGHTLGNALRRV
                     LLSSMPGHAPTEVQIAGVVHEYSTLDGVREDVVDILLNLKGVVFRLHNRAEAILSLKK
                     RGGEGNGAVTAADIEPAHDVEIVNPDHVIAHLAAGGKLEMQIKVESGRGYVPGNMRYL
                     PEETKGIGRIILDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGAIEPEEAIRY
                     SARVLVDQLSVFAQLEGTALPAEQPKSPAVDPILLRPVDDLELTVRSANCLKAENIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEHRP"
     gene            2267..>2364
                     /locus_tag="VFR57_04510"
     CDS             2267..>2364
                     /locus_tag="VFR57_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0258669.1"
                     /translation="MRHAHGLRKLNRTSSHRLAMLRNMTVSLLRHE"
BASE COUNT          476 a          791 c          758 g          339 t
ORIGIN      
        1 gctcaccgtc gagggcgatt tgcgccgcga ggtgcagatg tcgatcaagc gcctcatcga
       61 cctcagctgc tatcgcggct cgcgtcatcg caagggcctg ccggtgcgcg gacagcgcac
      121 ccgcacgaat gcgcgcacac gcaaggggcc tcgtaaggca gccatcaaac tcaacgtacc
      181 ggcagggaag gccgcataac ccatggcaag cagaaaccag cccacgcgcc aggagctgca
      241 gcagaccgct tcggcccgcg cgcgcaagaa ggtcaagaag aacgtcgccg aaggcattgc
      301 ccacatccac gcgtcgttca acaacaccat cgtcaccatc accgatcgcc agggcaatac
      361 gctcgcgtgg gcgacctccg ggcacgccgg gttcaaaggc tcgcgcaagt cgacgccgtt
      421 cgctgcgcag gtcgcggccg agcgctgcgg ccgcgcagcg caggagtgcg gcgtgaagaa
      481 catcgaagtg cgcatcaagg ggcccgggcc cgggcgcgaa tccgcggtgc gcgcgctgaa
      541 cgcgatcggc ctcaagatcg gcagcatcgc cgacgtgact cccattcctc acaacggttg
      601 ccggccgccc aagcggcggc gggtttagtt agtaatggcc agaaacctcg acaagtgccg
      661 gcattgccgg cgggagggcg agaagctctt cctcaaagga gagaagtgct tcaccgacaa
      721 gtgcccggtc gagcgccgcg cctacgcgcc cggccagcac ggccagaaga gcggcgcgcg
      781 gctatccgac tacggcaagc agctgcgcga gaagcagaag gtgcgacaca tctacggcct
      841 gctcgagcgc cagttccgca agacctacgc cgaggcggcg cgctccaagg gcgtgaccgg
      901 cgagcggctg ctgcagcttc tcgaatcgcg cctggacacc gtggtgtacc gcatgggctt
      961 cggtgcctcg cgcaccgagg cccgccagat cgtgcgccat aacggcatcc tggtgaacgg
     1021 caagcgggtc aatatcccgt cgtaccaggt gcgcgccggc gacgtgatcg aggtcgctgg
     1081 aggtgcgaaa gaacagctgc gcatcaaggc cgccgccgaa gcagcagcct cgcgcggcat
     1141 tccggaatgg atggaggtgg actccaaggg gctcaagggc acgttcaaga acctgcccgc
     1201 gcgcagcgac ctgccccaga cgattaacga aagcctcatc atcgagttgt actcgaagta
     1261 gagaaggact tacatgccgc aatcgggatt cctaaagccg cgtatcgttg acgttcagaa
     1321 tctgtcgccc aaccacgcac gcgtgacgat ggagcctttc gagcgtgggt acggccacac
     1381 ccttggcaac gctttgcgcc gcgtgctgct ctcgtcgatg cctggccacg cgccgaccga
     1441 agtgcagatc gcgggtgtgg tgcacgagta ctcgacgctc gacggcgtgc gcgaggatgt
     1501 ggtggacatc ctgctcaacc tcaagggcgt ggtgttccgg ctgcacaacc gcgccgaggc
     1561 gatcctgtcg ctcaagaagc gcggcggcga gggcaacggc gcggtgacgg ccgccgacat
     1621 cgagccggcg cacgacgtgg agatcgtgaa tcccgatcac gtgatcgcgc atctcgccgc
     1681 cggcggcaag ctcgagatgc agatcaaagt cgagtcgggc cgcggctatg tccccggtaa
     1741 catgcgctat ctgcccgaag agaccaaggg catcggccgg attattctgg acgcctcgtt
     1801 cagccccgtg cgccgcgtgt cctacgcggt cgagtcggcg cgcgtcgagc agcgcacgga
     1861 cctcgacaag ctgatcatgg atatcgagac caatggcgcg atcgagcccg aggaagcgat
     1921 ccgctattcg gcgcgcgtgc tcgtcgacca gctctcggtg ttcgcacagc tcgaaggcac
     1981 ggcgctcccg gccgagcagc cgaagtcgcc cgcagtcgat ccgatcctgc tcaggccggt
     2041 ggacgacctc gagctcacgg tgcgctcggc gaactgcctc aaggccgaga acatctacta
     2101 catcggtgac ctcatccagc gcaccgagac cgagctcctc aagacgccca acctcggccg
     2161 caagtcactc aacgagatca aggaagtcct cgcctcgcgc ggtctgacgc tcggcatgaa
     2221 actcgaaaac tggcctcccg ccggcctgga acatcgcccg taagccatgc ggcacgcaca
     2281 cgggctgagg aaactcaacc gcacctcgag ccaccggctc gcgatgctgc gcaacatgac
     2341 cgtgtcgctg ctgcggcacg agga
//
LOCUS       DASTPD010000127         5710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1290022, whole genome shotgun sequence.
ACCESSION   DASTPD010000127 DASTPD010000000
VERSION     DASTPD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290022"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1637)
                     /locus_tag="VFR57_04515"
     CDS             complement(93..1637)
                     /locus_tag="VFR57_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEU0258670.1"
                     /translation="MTLLLGRADVERLLPVDVCISAVEEGFRQHALGNVPAPGILGMR
                     AADGGFHVKAAFLGAYFAAKINANFPGNIGLPTIQGAVILFDSGNGRPLAIMDSISIT
                     ALRTAAASAVAAKYLARPESDTMLICGCGGQAASQLQALLRVRTPRRMFAYDREEGRA
                     RAFAARFDCIVVENLAEAARSSDIVVTCTTAERYFIAREMVRPGTFVAAVGADNERKQ
                     EIDPRLLADAKVVTDLTEQAARIGDLHHAIDAGAMAAADVHAELGEVIVGRKPGRERA
                     DEIIVFDSTGTGLQDVAAAVAVYGKALEEEGAMKGKLQTFAIAGAAALSPHARAEGFD
                     QAKIGSLPPGWECGVTGKGAPRWSVETDPTAPSAPQVLQQSGSGTFPWCVRKDTSLAD
                     GHVEVKFKPMRGREDQAGGVVWRWKDANNYYVARANALENNVSLYYTQNGRRNTLKYV
                     DAPVPLNAWHTLRVEFSGTRIRVALNGKVYIELEDGHLKGSGAVGVWTKADSVTAFDD
                     FDYAAQ"
     gene            complement(1634..2587)
                     /locus_tag="VFR57_04520"
     CDS             complement(1634..2587)
                     /locus_tag="VFR57_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEU0258671.1"
                     /translation="MRYTLKPLHCRPWLLQGLSPKLIESHYENNYGGALRRLNAITAQ
                     LEGADFAKMPGFTLNGLKREELIAHNSVVLHELYFASLGGDGRDPGSFAEVLVRDFGS
                     VDRWKAEFAAMGRALGGGSGWVLLTYVPREGRLVNQYASDHSQCAAGAIPILALDMYE
                     HAYHIDFGANAGAYVDTFLRNVDWKGVAARYQSASKVEALPDLVQKEFGDLPGVSVEE
                     VKGMIDSGQRVQFIDARPKHYVARTQDIIDGAAWRDPERVKEWAGELSKGDPVVVFCV
                     YGFHIGCQTAGALREAGFDAKYMKGGHAAWKAIGGPVKFFP"
     gene            complement(2773..3432)
                     /locus_tag="VFR57_04525"
     CDS             complement(2773..3432)
                     /locus_tag="VFR57_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258672.1"
                     /translation="MRYKLFSLCVAASCLFSGASAAEPFRYRVQPERERAWVIDAKGL
                     FLEEARRPRRSIALADWQWAAEPYACAPDIAIGPRGEAIVTSNVLPVLWKVDPDTLAV
                     SVHRLELDADTDKDVGFSSLVYSARHGAYFAVSEQHASLWRIDPLLRRAQKISAAAPA
                     RGACALTIERQERLSRPSTLCLHARGESWLVNLAPDQRSAYVQTVSCSFNPYTVLGGT
                     R"
     gene            3624..4583
                     /locus_tag="VFR57_04530"
     CDS             3624..4583
                     /locus_tag="VFR57_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEU0258673.1"
                     /translation="MFNALVLSLPTRNSTLRMRMWRALKDSGAAVLRDGVYLLPRDAA
                     EAGTLQRLQKEIRTHGGFAIVFDATPQAEDDLVALRARFDRTAEYATLVGRISAAKAS
                     LGALGPRRGLTALRRLEQALDKLGRIDFFPGEAKAQSAAALAALKARYGELYARNEPR
                     SSMKGLRPLERSRYQRRVWATRKAPWVDRLASAWLIKRFIDRDAKFAWIARPSECPRK
                     AVGFDYDGAEFTHVKNRVTFEVLAAAFELDRDPALAQIAAAVHFLDVGGIPVPDATGL
                     ETMLKGAKEKARGNDALLAEAMRTFDLLYSAYRANYSGMTPSA"
     gene            complement(4556..5425)
                     /locus_tag="VFR57_04535"
     CDS             complement(4556..5425)
                     /locus_tag="VFR57_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0258674.1"
                     /translation="MSRQAALVSCALIAALAANVSAQAQAPEGRLKKIADTRTLSIAY
                     RADAVPFSFVGDKGEPTGFSIDLCRRVASSIERELKVPGLKIEWVPVTVESRFEAVAK
                     GRADMECGSSTMTLSRMKQVDFSSAIFVETTGLLAKSVSGLRSISDLSRKRIAVVAGT
                     TNERAIHAHVQRRRLDATVLPFKSRDEALAALEEGKADAFASDRLLLVGAATKAKDPL
                     ALTLLPDQLSLEPYAIALPHGDAAMRLMVNTALSQVFAGDEITELLRRWFGALGRPSS
                     LLEAVYALGVIPE"
     gene            5507..>5710
                     /locus_tag="VFR57_04540"
     CDS             5507..>5710
                     /locus_tag="VFR57_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0258675.1"
                     /translation="MQILGICGSLRKASLNMVALRACEELMPQGMELRITSIADLPLF
                     NQDVFDAGIPEPCKRLRAEIAQAD"
BASE COUNT          989 a         1942 c         1829 g          950 t
ORIGIN      
        1 cacggcagcg agcgcaccaa cctcgcctac ggctacatca acaagggctg aagttttcgg
       61 caagaggccg gcgggcagcc cgccggcctt ttttattgcg cggcgtagtc gaagtcgtcg
      121 aaggcagtca cgctgtcggc cttcgtccac acgccgaccg cgcccgagcc cttgaggtgc
      181 ccatcctcga gctcgatgta caccttaccg ttcagcgcga cccggatgcg ggtgcccgag
      241 aactcgacgc gcaacgtgtg ccaggcgtta agcggcacgg gcgcgtccac atacttcagc
      301 gtattacggc ggccattctg cgtgtagtac agcgagacgt tgttctcgag cgcgttcgcg
      361 cgcgcgacgt aatagttgtt cgcgtccttc caccgccaca ccacgccgcc ggcctggtcc
      421 tcgcggcccc gcatcggctt gaatttcacc tcgacatgtc cgtcggcgag cgacgtgtcc
      481 ttgcgcacgc accacgggaa cgtgccagag ccgctttgct gcagcacttg gggcgcgctt
      541 ggcgcggtcg ggtccgtctc gactgaccag cgcggcgcgc ccttccccgt gacgccgcac
      601 tcccagccag gcggcaggct cccgatcttg gcctgatcga agccctcggc ccgcgcatgc
      661 ggggaaagcg cggccgcgcc ggcgatggcg aatgtctgaa gctttccttt cattgcaccc
      721 tcctcttcga gcgccttgcc ataaaccgcc actgccgcgg cgacgtcctg caagccggtg
      781 ccggtggaat cgaacacgat gatctcgtcg gcccgctcgc gtccgggctt gcgaccgacg
      841 atcacctcgc cgagctcggc atggacgtcg gcggccgcca tggcgcccgc gtcgatggca
      901 tggtgcaggt cgccgatgcg ggcagcctgc tcggtaagat cggttaccac cttggcatcg
      961 gcgagcagcc gcggatcgat ctcctgcttg cgttcgttgt cggccccgac cgctgcaacg
     1021 aaggtgcccg gccgtaccat ttcccgcgcg atgaaatagc gctccgcggt cgtgcacgtc
     1081 acgacaatgt cgcttgagcg cgccgcttcg gcgaggttct ccactacgat gcaatcgaag
     1141 cgcgcggcaa acgcgcgtgc cctgccctcc tcgcgatcgt aagcaaacat gcgccgcggc
     1201 gtgcgtacac gaagcagcgc ttgaagctgg gatgcggcct ggccgccgca tccacatatg
     1261 agcatcgtgt ccgattccgg ccgcgccagg tacttcgcgg ccactgcgct tgccgcagca
     1321 gtgcgaaggg ctgtaataga gatcgaatcc atgatcgcca gcgggcggcc gttgcccgag
     1381 tcgaaaagga tcaccgcgcc ttggatcgtc ggcaggccga tgttgccggg gaaattggcg
     1441 ttgatcttgg cggcgaagta agccccgaga aacgccgcct tgacgtggaa gccgccatcg
     1501 gccgcacgca tgccgagaat cccgggtgcg ggaacgttgc ccagcgcgtg ctgccggaat
     1561 ccttcctcga ccgcgctgat gcagacatcc accggtagga ggcgctctac gtcggcgcga
     1621 cccagcagca atgtcatgga aagaacttca ccggcccgcc gatcgccttc catgccgcat
     1681 gcccaccctt catgtacttc gcatcgaagc ccgcctcccg cagcgcaccg gcggtctggc
     1741 acccgatgtg gaaaccgtac acacagaaga cgacgaccgg gtcgcccttc gacagctcgc
     1801 cggcccactc cttgacacgc tcgggatcgc gccacgccgc gccgtcgatg atgtcctgag
     1861 tgcgcgcgac ataatgcttc ggccgcgcgt cgatgaattg gacgcgctgc cccgagtcaa
     1921 tcatgccctt cacttcttcg acgctgacgc ccgggagatc gccgaactcc ttctgcacca
     1981 gatcgggaag cgcctcgacc ttcgatgcgc tctggtagcg cgcggcgacg cccttccagt
     2041 cgacgttgcg cagaaaggta tcgacgtagg cgccggcgtt cgcgccgaag tcgatgtggt
     2101 aggcatgctc gtacatgtcc agggccagga tcggaattgc gcctgccgca cactggctgt
     2161 gatccgacgc atactggttc accagccgcc cctcgcgggg cacataggtc agcaggaccc
     2221 agcccgatcc cccgcccagc gcgcggccca tagccgcaaa ctcggccttc cagcggtcca
     2281 ccgagccgaa gtcgcggacc agcacttcgg cgaagcttcc ggggtcgcgc ccgtcgcctc
     2341 caaggctggc gaaatagagt tcgtgaagga ccaccgagtt gtgcgcgatc aattcctcgc
     2401 gcttgaggcc attcagcgtg aagcccggca tcttggcgaa atcggcgccc tcgagctgag
     2461 ccgtgatggc gttcagacgc ctgagagctc cgccgtagtt gttctcatag tggctctcga
     2521 tgagcttcgg cgagagcccc tgcagcagcc acggacggca gtgcagtggt ttcaaggtgt
     2581 agcgcatgat catctctcct ttaaacgaag tcacgcagac gcatcgcgaa gcgggaacgc
     2641 gccacaatgc ctagtgctgc tccgctcagc gccagcgcaa gcgcgggcag cttccacaac
     2701 agcagtgctg cgatcgtgag aacgaaaagc gcgcccgtaa atacgtcacc tcaaggcgtc
     2761 tacgcatcac cgtcatcgcg tgcctccgag cacggtgtac ggattgaaag aacagctgac
     2821 cgtctgcacg taggccgagc gctggtcggg cgcaagattc acgagccagg attcgcccct
     2881 tgcatgcagg cacagtgtgc tcgggcgact gaggcgttcc tgacgctcga tcgtcaatgc
     2941 gcacgcgccg cgtgcgggcg cggcggccga gatcttttgc gcgcggcgca gcagcgggtc
     3001 gatgcgccag agcgacgcgt gctgttcgct gacggcgaaa taggcgccgt ggcgcgccga
     3061 gtacacgagc gacgaaaagc cgacgtcctt gtccgtatcc gcatcgagct cgaggcggtg
     3121 taccgaaacc gcaagagtgt caggatcgac tttccacagc accggtagca cgttgctcgt
     3181 gacgatggct tcgccccgcg ggccgatggc gatgtccggc gcgcaggcat agggctcggc
     3241 cgcccattgc cagtcggcga gcgcgatgct gcggcgcggc ctgcgtgcct cttcgaggaa
     3301 taaccccttg gcatcgatca cccacgcccg ctcacgctcg ggctgcacgc ggtagcggaa
     3361 cggttcggca gccgaagcgc cggagaaaag gcacgaggcc gcaacgcaga gcgagaagag
     3421 cttgtaacgc atgacgcact cctttcccat tcgggaaatc ggagcagcgc ttgagacgtg
     3481 atgtcttgac tgccgggtgt ctgcctgagg acccggccag ggcgggcgcc ctgctagaag
     3541 gcaccttagg cgcggcggcc cggcgcgtca acggtgccgg gccattcttt ggcacgttct
     3601 tgtgatacgt ataacatggg gtgatgttca acgccctggt cctcagcctg ccgacgcgca
     3661 attccacctt gcgcatgcga atgtggcgcg cgctcaagga ctccggcgcc gcggttttgc
     3721 gcgacggcgt gtacctcctg ccccgggatg ccgccgaagc tggcacgctc cagcggctgc
     3781 aaaaggaaat ccgcacccac ggcggcttcg ccatcgtctt cgatgccaca ccgcaggcag
     3841 aagacgacct cgtcgccttg cgtgcacgct ttgatcgaac ggcggaatat gcaacgctcg
     3901 tcggcagaat ttcagccgcg aaggcgtcgc tcggcgccct agggccgcgg cgcggactca
     3961 ccgcgttgcg ccgtctcgag caggcgctcg acaagctcgg gcgcatcgac ttctttcccg
     4021 gcgaagcgaa agcgcagagc gccgccgcgc tggcggcgct caaggctcgc tatggcgagc
     4081 tctacgcgcg gaacgagccg cgctcctcga tgaagggcct gcggccgctc gagcgctcgc
     4141 gctaccagcg gcgcgtctgg gcaacccgca aagcgccctg ggtcgatcgc ctcgccagcg
     4201 cctggctcat caagcgcttc atcgaccgcg acgcgaagtt cgcctggatc gcccggccga
     4261 gcgagtgccc gaggaaggcg gtgggcttcg actacgacgg cgcggaattc acccatgtca
     4321 agaaccgggt cacgttcgag gtgctggccg cggcattcga gctcgatcgc gacccggcgc
     4381 tcgcgcagat cgcggccgcc gtccacttcc tcgacgtcgg cggcatcccg gtgccggatg
     4441 cgaccggtct cgaaacgatg ctgaagggcg cgaaggagaa ggcgcgcggc aacgacgcct
     4501 tgctcgccga ggcgatgcgg accttcgacc ttctttactc cgcctatcgc gcgaattatt
     4561 cggggatgac gccgagcgcg taaacggctt cgagcaacga ggacggacga cccagcgcgc
     4621 cgaaccagcg gcgcaggagc tccgtgatct cgtcgccggc gaacacctgg gaaagggcgg
     4681 tgttgaccat gaggcgcatc gccgcgtcgc cgtgcggcag cgcgatggcg tagggctcga
     4741 gcgagagctg gtccggcaag agcgtcagcg ccagcgggtc cttcgccttg gtcgcggcgc
     4801 cgacgaggag cagccggtcg ctcgcgaaag cgtcggcctt gccctcctcg agcgcggcaa
     4861 gggcctcgtc gcgtgacttg aagggcagca cggtggcgtc cagccgccgg cgctggacgt
     4921 gcgcatggat ggcgcgctcg ttggtcgtgc ccgccaccac cgcgattctt ttccgggaaa
     4981 ggtcggagat cgagcgcagg cccgagacgc tcttcgcgag cagtccggtg gtctccacga
     5041 agatcgcact cgagaaatcg acctgcttca tgcgcgaaag cgtcatagtg ctcgagccgc
     5101 actccatgtc cgcacgcccc ttcgccaccg cctcgaagcg cgactcgacc gtcaccggca
     5161 cccattcgat cttcaggccc gggaccttga gctcgcgctc gatcgagctc gctacgcgcc
     5221 ggcaaaggtc gatggagaac ccggtcggct cacccttgtc gccgacgaag gaaaacggca
     5281 ccgcgtcggc gcggtaggcg atcgacaggg ttcgcgtgtc ggcgatcttc ttcaggcgcc
     5341 cttcgggcgc ctgcgcttgg gccgaaacgt ttgcggcgag cgctgcgatc agcgcgcaag
     5401 acaccaaagc ggcttgcctg gacatgcgac tctcctcagt gaaagcaggc ggcatgctag
     5461 gagggacgcc cgttctcgcc tatagaacct tggtagactc gcccgcatgc agatcctcgg
     5521 catctgcggc agcctgcgca aagcctctct caacatggtc gcgttgcgcg cctgcgagga
     5581 gctcatgccg cagggcatgg agcttcgcat caccagcatc gccgacctgc cccttttcaa
     5641 ccaggacgtg ttcgacgccg gcatcccgga gccgtgcaag cgcctgcgcg ccgagatcgc
     5701 gcaggccgac
//
LOCUS       DASTPD010000128         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1698439, whole genome shotgun sequence.
ACCESSION   DASTPD010000128 DASTPD010000000
VERSION     DASTPD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698439"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..281)
                     /locus_tag="VFR57_04545"
     tRNA            complement(206..281)
                     /locus_tag="VFR57_04545"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:cgc)
     gene            418..723
                     /locus_tag="VFR57_04550"
     CDS             418..723
                     /locus_tag="VFR57_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258676.1"
                     /translation="MVPKERRKRRLGLKVIHKHRVGDKVLRRVSELVIIEGQPKALLG
                     WINFGGVRTPIYLCDLDLAKLHRSATSTSTFYYEEITVDPRFEGLAPGQQDDPLQKG"
     gene            complement(734..>2789)
                     /locus_tag="VFR57_04555"
     CDS             complement(734..>2789)
                     /locus_tag="VFR57_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU0258677.1"
                     /translation="LKSLRARLPDEKEPWVRIGSLEMTGARVEPGAQTANLGEIAIGD
                     VTLRLRRSADGVFNAARIAKADRSEKNEQPGLPWRIDLARATLQRGALTFEDLALGEP
                     TATISIAPLEVKAEKLSTAKGASGSINVRATIDKKGTLAAAGPLTLDPLGARLNIVAQ
                     TIGLLPAQRYIDDKVHFAITSGRLSAKGTALFELPPGRALKAAYRGGVEVTDFASIDK
                     RSTQDLLRWKALSLAAIDFELEPLKLSVDEIGLSEYYARIIVTADGALNLQALLKDPE
                     STAQAPAPQATVASASNVRIGRITARDGNVNFSDFFIRPNFSANLTGFGGSVTEMTPE
                     KAGDVELHGKIDNAAPVEIAGRVNLLGKDLFLDMKASARDIELAPLSAYAVKYAGYGI
                     QRGKLSVALKYGIENRKLSAENQIYLDQLTFGERVESPTATTLPVLLAVALLKDRNGV
                     IDVNLPISGSLDDPQFSVAGIIFQVIGNLVVKAVTSPFALLGSLVGGGEELSFVEFAP
                     GSAALSAGEQQKLGSLAKALTERPNLRLEITGRAAADPDENALKRAALDAKVRTQKFD
                     ELRLAGTAPASPDSVTVDAAEYERYLRRAYGAQKLPDKPRNFLGIAKELPVPEMEGLM
                     LAHTNIDKDDLQRLANSRAQVAKEWLMTEGKVPAERMFINAPVLAAPEKGKPTRVDFG
                     LR"
BASE COUNT          475 a          885 c          899 g          530 t
ORIGIN      
        1 agctgtcgag ctctccgaga tcaggtcgcg gccaagagga agcttgcagc actcgcgcgc
       61 gtcatgggct ttggcacact tggtgcggtc agatggcagc attccgcagc agactgcaac
      121 gctgccgcgt gtcagctgag gggatgatcc gcttcgaccc gcgcgcgaag tcttagctac
      181 cgccggatac tgctagaaag agaattggtg gagtggagga ggatcgaact cccgaccttc
      241 gcattgcgaa cgcgacgctc tcccagctga gctaccaccc cgcagagcgg cgaattctac
      301 cgcaacatgc tttgcatcct agaccaagct ctacggggtg taacgacccg gactacatac
      361 ggtcaatgat ctgagcgcag cggcacgact cctgctccat tcgcctcgga gcccggaatg
      421 gtgcccaagg agcgcaggaa aaggcggctg gggttgaagg tgatccacaa gcaccgggtc
      481 ggcgacaagg tcctgcgcag ggtctcggag ctcgtaatca tcgaagggca acccaaggca
      541 cttctgggct ggatcaattt cggcggcgtg cgtaccccga tctacctctg cgaccttgac
      601 ctcgcgaaac tgcaccgctc agcgactagc acgagcactt tttactacga agaaatcacg
      661 gtggatccgc gcttcgaggg gctcgcgccc gggcaacagg atgacccgct gcaaaagggc
      721 tgaggccgct cgctcacctc aatccgaaat cgacgcgcgt gggtttgccc ttctccggcg
      781 cggccagcac cggggcgttg atgaacatcc gctctgccgg caccttgcct tcggtcataa
      841 gccattcctt ggcaacctgc gcgcggctgt tcgccagccg ctgcagatcg tccttatcga
      901 tgttcgtgtg agcgagcatc agaccctcca tttccggaac cggaagctcc ttcgcgatgc
      961 caaggaaatt gcgcggcttg tcgggaagct tttgcgcccc gtaggcgcgc cttaggtacc
     1021 tttcgtactc cgcggcgtcc acggttaccg aatccggcga cgcaggcgcc gttccggcga
     1081 ggcgcagctc gtcgaacttt tgggtcctca ccttggcatc gagcgcggcg cgcttgagag
     1141 cgttctcgtc cggatccgcc gctgcgcggc cggtgatctc aagccgcaag ttcggacgct
     1201 cggtgagcgc cttggcgagg ctgccgagct tctgctgctc gccggcggag agcgcagccg
     1261 accccggggc gaattcgaca aagctcagct cttcgccgcc gccgacgagc gagccgagca
     1321 gcgcgaaggg tgaagtgacc gccttcacca ccaggttccc gatcacctga aagatgatgc
     1381 cggcgacgct gaactggggg tcgtccagcg atcccgagat cgggaggttg acgtcgatca
     1441 cgccgttgcg gtccttgagc agcgcgacgg ccagcagcac cggcagcgtg gttgcggtcg
     1501 gactttccac gcgctcgccg aaggtgagct ggtcgaggta gatctggttc tcggcgctga
     1561 gctttcggtt ctcgatgccg tacttgagcg cgaccgagag cttgccgcgt tgaatgccgt
     1621 agcccgcgta ctttacggcg tacgccgaga gcggcgcgag ctcgatgtcg cgcgcactcg
     1681 ccttcatgtc gagaaagaga tccttgccga gcaggttcac gcggccggcg atctcgaccg
     1741 gcgcggcatt gtcgatcttg ccgtgcagct cgacgtcgcc cgctttctca ggcgtcattt
     1801 cggtcaccga gccgccgaag ccggtcaggt ttgcgctgaa gttcggccga ataaaaaagt
     1861 cgctgaaatt gacgttgcca tcgcgcgccg taattctccc gatgcgcacg ttcgaagcgg
     1921 aagccactgt cgcctgcggt gccggggcct gggccgtcga ctccggatcc ttcagcagcg
     1981 cctgcaggtt gagggcgccg tcggccgtga cgatgatgcg cgcgtagtac tcggagagtc
     2041 cgatctcgtc gaccgatagc ttgagcggct cgagctcgaa atcaatcgcc gcgagcgaaa
     2101 gcgctttcca cctcagcagg tcttgggtcg agcgcttatc gatggaggcg aaatcggtta
     2161 cctcgacgcc tccgcggtat gcggccttga gcgcccggcc cggcggcagc tcgaacaacg
     2221 cggtgccttt cgccgacagg cggcccgaag tgatggcgaa gtgcaccttg tcgtcaatgt
     2281 agcgctgcgc gggcaggagg ccgatggtct gcgcgacgat gttgagacgt gcaccgagcg
     2341 gatcgagggt gagcggtccg gctgcggcga gcgtgccttt cttgtcgatc gtggcgcgca
     2401 cgttgatgct gccgctagcg cccttggcgg tcgaaagctt ctcggctttg acctcgagcg
     2461 gcgcgatgct gatcgtcgcc gtcggttcac cgagtgcgag gtcttcgaac gttaaggcac
     2521 cgcgctgcaa cgtcgcgcgc gcaaggtcga tgcgccacgg taaccccggc tgctcgttct
     2581 tctccgatcg atccgctttc gcaatgcgcg cggcgttgaa aacgccgtcc gcgctgcggc
     2641 gcagccgcag cgtcacgtcg ccgattgcga tttcgccaag gtttgcggtc tgcgcaccgg
     2701 gctcgacgcg cgcgcctgtc atttccagcg agccgatgcg gacccatggc tccttttcgt
     2761 ccggcagccg tgcgcgcagg cttttcagc
//
LOCUS       DASTPD010000129         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_67379, whole genome shotgun sequence.
ACCESSION   DASTPD010000129 DASTPD010000000
VERSION     DASTPD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67379"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFR57_04560"
     CDS             complement(<1..824)
                     /locus_tag="VFR57_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0258678.1"
                     /translation="MWSYILKRLLLMIPTLFGILLITFIVIQFVPGGPVEQMVAQFQG
                     FEAGGEGPAAAEGGYRGRQGVDAVRVEEIKKLYGFDKPAPERFVQMIGQFARFDLGQS
                     FFHNKAVGQLILEKLPVSISLGLWTFLLVYLISVPLGIAKAVRAGTAFDTMTTFIVLL
                     GYAIPSFVMGVVLLVFFAGGSFFQWFPLRGITSMNWHELGIPGKILDYFWHIALPVTA
                     MVLGGFAVITILTKNSVLEEIRKHYVLTARAKGVDERRVLWRHVFRNAMIPIMTGFP"
     gene            complement(825..2609)
                     /locus_tag="VFR57_04565"
     CDS             complement(825..2609)
                     /locus_tag="VFR57_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0258679.1"
                     /translation="MKFPALLAALFWPTSLWAAHAYSQFGDIKYPPGFSHFEWVNPSA
                     PKGGEIELVPPLRITNFDKYNPFTLKGTAPPGTGLLFESLLTGTMDEPTTAYGLLAED
                     VEVAPDGLSATYRINPAARFHDGTPVTAADAKHSFDMLMSKQAAPQYRVIYSDIKQAI
                     VVGPRTIRYEFKRPNAELPLMAGGLPVFSRAWGAGKPFDQIVMDIPIASGPYKIGRLN
                     FGRDITYERDPNYWARELNVRRGRYNFDRVTYKIYKDTTAQTEAFKAGEFDYLRTFSA
                     REWARTYVGKKFDSGELVKVELPTKNAGDFQGFLINTRRAKFKDPRVRQALGLAFDFE
                     WMNRQMMYNAYTRVRGFFNASDFEAKGMPGPDELKVLESLRANLPEKVFAEEVPMPPS
                     THPPGSLRDNLRKARDLLAEAGWTYRDGALRNAKGEPFTLEYLDSGGGERLVAPYFQA
                     LAKLGITGEYRRADFALIRKRLDVFDYDIFTVRIPGNESPGAELLDRFGSQSADTEGS
                     SNLIGVRDPAIDALVSLAVSSRTRPELVARLRALDRVLRHQYFVIPQWYASTFRIAYR
                     AGKFDQPKVMPQYYQAEDWVISTWWKKQ"
     gene            complement(2606..>3965)
                     /locus_tag="VFR57_04570"
     CDS             complement(2606..>3965)
                     /locus_tag="VFR57_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258680.1"
                     /translation="YDGKSLLLASGSEMRRLRGAGISMIFQEPMSSLNPVFSVGFQIG
                     EALREHLGMSRAETRRRTLELLQEVGISQPQRKIDAYPFQLSGGEQQRVMIAMAIACA
                     PKLLIADEPTSALDVTVQKQILDLIAELQRRRRMAVLFITHDLGLAGEIASRVLVMHQ
                     GVIREQGSVADVFGAPRDAYTRALLAARPRLDQPRRPSARWDDTVVLEARGLEKRFDT
                     VSAVKGASFKLRKGRTLGLVGESGSGKTTLGLLVARLHQASAGSALLHGRDMLSVPPK
                     AFKRSVQIVFQNPYASLNPRFSIGQTLTEPMRIHAIGADERERHALAAGVLAKVGLPA
                     DALLKYPHQFSGGQRQRIAIARALVLRPELLVCDEAVSALDVSIQAQILKLLQDLQDE
                     TAMSYLFISHDLAVVRSMADEVMVMKDGEIVEAAPADEIYSQPRHPYTRTLLASVPGN
                     RK"
BASE COUNT          703 a         1273 c         1292 g          697 t
ORIGIN      
        1 gggaagccgg tcatgatcgg gatcatggcg ttgcggaata cgtgccgcca cagcacccgc
       61 cgctcgtcca cgcccttcgc gcgcgcggtg agcacgtaat gcttgcggat ctcctcgagc
      121 accgagttct tggtgaggat ggtgatgacg gcgaagccgc cgagcaccat cgccgtcacc
      181 ggcagcgcta tgtgccagaa gtagtcaaga atctttccgg gaatgccgag ctcgtgccag
      241 ttcatggatg tgatgcctcg caacgggaac cactggaaaa aagaaccgcc ggcgaagaag
      301 acgagcagca cgacgcccat gacgaagctc gggatggcgt agccgagcag cacgatgaac
      361 gtcgtcatcg tgtcgaacgc agtccccgcg cggaccgcct tggcgatgcc aagcggaacc
      421 gaaatcaggt acacgaggag gaaggtccac agccccaggc tgatcgacac cggcagcttc
      481 tcgaggatca gctgcccgac ggccttgtta tggaagaagc tctggcccag gtcgaagcgc
      541 gcaaactggc cgatcatctg cacgaagcgc tcgggcgccg gcttgtcgaa gccgtagagc
      601 ttcttgatct cctcgacgcg caccgcgtcg acgccctggc gcccgcggta gccgccctcc
      661 gccgccgccg gcccttcgcc gccagcctcg aagccctgga actgggccac catttgctct
      721 accggcccgc cgggaacgaa ctggatcacg atgaacgtga tgagcaggat cccgaacagg
      781 gtcgggatca tgagcagcag acgcttgagg atgtacgacc acatctattg ttttttccac
      841 caagtcgaaa tcacccagtc ctcggcttga tagtactggg gcatcacttt cggctgatcg
      901 aacttccccg cgcggtatgc gatgcggaac gtgctcgcat accactgcgg aatgacgaag
      961 tactggtggc gcagcactcg gtcgagcgcg cgcaggcgcg ccacgagttc cggccgcgtg
     1021 cgcgaagaca ccgcgagact gacgagcgcg tcgatcgccg ggtccctgac tccgatcagg
     1081 ttgctcgagc cctcggtgtc ggccgactgc gagccgaagc ggtcgaggag ttcagccccg
     1141 ggcgactcgt ttcccgggat gcgcaccgtg aagatgtcgt aatcgaaaac atcgagccgc
     1201 ttgcggataa gcgcgaagtc ggcacggcga tactcgcccg tgatgccaag cttggcgagc
     1261 gcctggaaat aaggcgcgac cagccgctct ccgccgccgc tgtcgagata ttcgagcgtg
     1321 aacggctcgc ccttggcgtt gcgcagcgcg ccgtcgcggt aggtccagcc tgcctcggcc
     1381 agcaggtcgc gtgccttgcg caagttgtcg cgcaggctgc ccggcgggtg ggtcgacggc
     1441 ggcatcggga cttcttcggc gaacactttt tcgggaaggt ttgccctaag agactcgagc
     1501 accttgagct cgtccggccc cggcatgccc ttggcttcga agtcgctcgc gttaaagaag
     1561 ccgcgcacgc gcgtgtaggc gttgtacatc atctgccggt tcatccactc gaagtcgaag
     1621 gcgagcccga gcgcctggcg cacccgcggg tccttgaact tggcgcgccg cgtattgatg
     1681 aggaagccct ggaagtcgcc cgcattcttc gtcggcagct cgaccttgac gagctcgccg
     1741 gaatcgaact tcttgccgac gtaggttcgc gcccattcgc gggccgagaa agtacgcagg
     1801 tagtcgaact cgccggcctt gaacgcctcg gtctgggcgg tggtgtcctt gtagatcttg
     1861 taggtaacgc ggtcgaagtt gtagcggccg cggcgcacgt tgagctcgcg cgcccagtag
     1921 ttgggatcgc gctcgtacgt gatgtcgcgg ccgaagttga gccgtccaat cttgtacggt
     1981 ccactcgcga tcgggatgtc catgacgatc tggtcgaacg gcttgcccgc gccccaggcg
     2041 cggctgaaca ccggcagacc gcccgccatg agcggcagct cggcgttcgg gcgcttgaac
     2101 tcgtagcgga tcgtgcgcgg cccgacgact atcgcctgct tgatgtcact gtagatcacc
     2161 cggtactgcg gcgccgcctg cttgctcatg agcatgtcga agctgtgctt ggcgtcggcc
     2221 gccgtgaccg gcgtgccgtc atgaaagcgc gccgccgggt tgatgcggta ggtggccgag
     2281 aggccgtcgg gcgccacttc gacgtcctcg gccagcagcc cgtaagccgt ggtcggctcg
     2341 tccatcgtgc ccgtcagcag gctctcgaag agcagcccgg tgccgggcgg cgcggtacct
     2401 ttcagcgtaa acgggttgta cttgtcgaag ttcgtgatgc gtagcggcgg cacgagctcg
     2461 atctctccac ccttcggcgc gctcggattc acccactcga agtgcgagaa acccggcggg
     2521 tacttgatgt cgccgaactg cgaataggcg tgcgccgccc atagggaagt cggccagaac
     2581 aaggcagcca gtaaggcggg gaacttcatt tgcggttgcc gggaaccgaa gcgagcaagg
     2641 tacgggtgta aggatgcctg ggttgcgagt agatttcgtc ggccggcgcc gcctcgacga
     2701 tctcgccgtc cttcatcacc atgacctcgt cggccatgga ccgtactaca gcgaggtcgt
     2761 gggagatgaa gagatagctc atcgcagtct cgtcctgcaa gtcctgcagc agcttcagga
     2821 tctgcgcctg gatggatacg tcgagcgccg acaccgcctc gtcgcacacg aggagctcgg
     2881 gtctcaacac gagtgcccgg gcgatggcga tgcgctgccg ctggcctccg gaaaactggt
     2941 gagggtactt gaggagcgcg tcggccggca ggcccacttt cgccaagacc cccgccgcca
     3001 gcgcgtggcg ctcgcgttcg tcggcgccga tggcatgaat tcgcatcggc tcggtgagcg
     3061 tctggccgat gctgaagcgc gggttgagcg aggcgtaggg gttctggaac acgatctgca
     3121 cgctgcgctt gaaggccttg ggcggaaccg aaagcatgtc gcgcccgtgc agaagagccg
     3181 agcccgcaga agcctggtga agccgggcga cgaggagacc gagggtcgtc ttgcctgagc
     3241 cggattcgcc cacgagcccg agggtccggc ccttgcgaag cttgaagctt gcgcccttca
     3301 ccgcgctcac cgtatcgaaa cgcttttcca gaccgcgcgc ctcaagaaca accgtgtcgt
     3361 cccaccgcgc gcttggccgc cgcggctggt cgagccgcgg acgagccgcg agcagcgctc
     3421 gcgtataggc gtcgcgcggt gcgccgaaaa cgtccgcgac gctcccctgc tcccggatca
     3481 caccctgatg catcaccagg actcggcttg cgatctcccc ggccaggccg aggtcgtgag
     3541 tgatgaagag caccgccatc cggcgccggc gctgcagctc ggcgatcagg tccaagatct
     3601 gcttctgcac cgtcacgtcg agcgcgctcg tgggctcgtc ggcgatgaga agcttcggcg
     3661 cgcaagcgat cgccatggcg atcatgacgc gctgctgctc gccgcccgag agctggaacg
     3721 gatacgcatc gatcttgcgc tgcggttgcg agattcccac ttcctgtaac aactccagcg
     3781 tccgtcgccg cgtctcggcg cgcgacatcc ccagatgctc acgcagggcc tcgccgatct
     3841 ggaagccgac gctgaacacc gggttgagcg agctcatcgg ctcctggaag atcatcgata
     3901 tgcccgcgcc ccggagccgt cgcatttcgc ttcctgatgc caaaagaagg ctctttccgt
     3961 cgtag
//
LOCUS       DASTPD010000130         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1701434, whole genome shotgun sequence.
ACCESSION   DASTPD010000130 DASTPD010000000
VERSION     DASTPD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701434"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFR57_04575"
     CDS             complement(<1..292)
                     /locus_tag="VFR57_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258681.1"
                     /translation="MRKLIGAVALAAAALAHAQSWPNKPIKYIVNFAPGGTTDILGRM
                     VAAGLTSSLGQPVVVENKPGQAGSIGAAECARAAPDGYTLCGGTISSHAINAS"
     gene            complement(314..1252)
                     /locus_tag="VFR57_04580"
     CDS             complement(314..1252)
                     /locus_tag="VFR57_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0258682.1"
                     /translation="MKPAIIITAKGHAGTMATLQSEFDAHLLSDAPDRTAFLKQHSSK
                     VRGLATFGPMPVDGKLMDALPKLEIISNFGVGVDAINLDDAKKRKIIVTNTPDVLNEC
                     VADTALALVLNTVRKFPQSEKYLRAGHWASRGPYPLTTSLGGKTLGILGLGRIGEAIA
                     KRVSACGMKIRYHNRNKKDVPYPYDPDPVTLAKNCDVLMVVTPASAETNRIINAKVLD
                     ALGPKGYLVNIARGSVVDEPVLLRYLQEGKIAGAGLDVYADEPRVPPEFFTLDNAVLF
                     PHVGSATVETRKAMGDLQIENLRRHFAGKPVLTSVV"
     gene            complement(1249..2016)
                     /locus_tag="VFR57_04585"
     CDS             complement(1249..2016)
                     /locus_tag="VFR57_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEU0258683.1"
                     /translation="MNLPENAFKRALRAGTPQIGLWSSLSSNYTVEVIAGAGFDWILL
                     DSEHSPADIENLLTQLQAAAPYPTHPVVRIPWNDMVTIKRVLDIGAQSLLVPYVSTAD
                     EAKAAVSYTRYPPAGVRGVAGTTRATRFGRIPDYARRAHEQICLLVQVETKGALDNIE
                     AICGIDGVDGVFIGPADLHASLGHPGEIANPNVKPMIDNAIRRIRKAGKAPGILTPNE
                     ADARHWLDCGALFVAVGADVGILARGAEALAAKFKRS"
     gene            complement(2013..2969)
                     /locus_tag="VFR57_04590"
     CDS             complement(2013..2969)
                     /locus_tag="VFR57_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258684.1"
                     /translation="MKRIAFLTLALCAAGASAQSYPTKPIRIVVPYAAGGTSDILARQ
                     IGPELNKAWGQPVVVENKPGANGNVGADFVAKSAPDGYTLLLTDLGGLVISASVYPKL
                     PFNPSKDFAPVVMVSYSPHVLAVHPEVKVKDVKELVAMAKAYPGKLNFAMSGIGGAPH
                     LAGIEFAQRMGIDWTYIPYKGGADAITGVVGGQAHVLFNGMLATWPHVTGGRLKALAI
                     SSAQRVPSAPDTPTVAEQGLPGFETGSYQGVVGPVGIPRETVTKLNGELRKILNTQDI
                     KDRFAKQGTEVRTDTPESLGKWMTTEQAKWAKVVKESGAKFE"
     gene            complement(2980..>3285)
                     /locus_tag="VFR57_04595"
     CDS             complement(2980..>3285)
                     /locus_tag="VFR57_04595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0258685.1"
                     /translation="RMSGTSYGCCVLHVAPESFVGGPLALVKNGDPIELDVEKRELNL
                     RISEEEMKKRRAAWRQPSKKFERSYGAIFSRHVKQADEGCDFDFLEGTAPVPEPEIH"
BASE COUNT          501 a         1068 c         1090 g          626 t
ORIGIN      
        1 ggctcgcgtt gatcgcgtgc gagctgatag ttccgccgca tagcgtgtag ccgtccggcg
       61 cggcgcgcgc gcactcggcg gcgccgatcg accccgcctg cccgggcttg ttctccacta
      121 ccaccggctg cccgagcgag ctcgtgaggc cggccgcgac catgcgtccg aggatgtccg
      181 tcgtgccgcc gggggcgaag ttgacgatgt acttgatggg cttgttgggc cagctttggg
      241 cgtgcgccag cgccgcggcc gcgagggcga cggcgccgat cagctttctc atctggcgaa
      301 cctcctcctt ggcttaaacg acgctggtca gaaccggttt accggcaaag tggcgccgca
      361 ggttctcgat ctgcaggtcg cccatcgcct tgcgcgtctc cacggtggcg ctgccgacgt
      421 ggggaaagag caccgcgttg tccagggtga agaactccgg cggtacgcgc ggctcgtcgg
      481 cgtagacgtc gagcccggca ccggcgatct tcccctcctg caggtagcgc agcaggaccg
      541 gctcgtccac gaccgaaccg cgggcgatat ttacgagata gcccttcggg ccgagcgcat
      601 ccagtacctt cgcgttgatg atgcgattgg tctcggcgct cgccggcgtc accaccatga
      661 ggacgtcgca gttcttcgcc agcgtgaccg ggtccggatc gtagggatag ggcacgtcct
      721 tcttgttccg gttgtggtag cggatcttca tgccgcaggc agatacgcgc ttggcgatcg
      781 cctcgcctat acgccccaga cccaggatgc cgagggtctt gccgccgagc gaggtggtga
      841 gcgggtaagg gccgcgcgaa gcccagtggc ccgcgcgcag atatttctcc gactgcggaa
      901 acttgcgcac cgtgttgagc acgagcgcaa gcgcggtgtc cgcgacgcac tcgttcagca
      961 cgtcgggcgt gttggtgacg atgatcttgc gcttctttgc gtcgtcgagg ttgatcgcgt
     1021 ccacgccgac gccgaagttc gagatgatct cgagcttcgg cagcgcgtcc atcagcttgc
     1081 catcgaccgg catcgggccg aacgttgcaa ggccgcgcac cttggaagaa tgctgtttga
     1141 gaaaggcggt gcggtcgggc gcatccgata gcagatgggc atcgaactca gactgcaggg
     1201 tggccatcgt gccggcgtgg cccttggcgg tgatgatgat cgcgggcttc atgatctctt
     1261 gaacttggcg gcgagcgcct cggccccgcg cgcgaggatg ccgacgtccg cgccgaccgc
     1321 gacgaacagc gcaccgcagt cgagccagtg acgcgcgtcg gcctcgttcg gggtcaggat
     1381 gccgggcgcc ttgccggctt tgcggatgcg gcggatggcg ttgtcgatca tcggctttac
     1441 gttcgggttg gcgatctcgc cggggtggcc gagcgaggca tgcaggtcgg ccggtccgat
     1501 gaacacgcca tcgacgccgt ctattccgca gatcgcctcg atgttgtcga gcgctccctt
     1561 cgtctcgacc tgcacgagga ggcagatctg ctcgtgcgcg cggcgcgcgt agtccgggat
     1621 gcgcccgaaa cgcgtggcgc gcgtcgtgcc ggcgacaccg cgcacaccgg cgggcggata
     1681 gcgcgtgtac gacaccgcag ccttcgcttc gtccgcggtc gacacgtaag gcactaacaa
     1741 ggattgcgcg ccgatgtcga gcacgcgctt gatcgtcacc atgtcgttcc agggaatgcg
     1801 caccaccggg tgcgtcgggt agggcgccgc tgcctgcagc tgggtgagca gattctcgat
     1861 atcggcaggc gaatgctcgc tgtcgagcag aatccagtcg aacccggcgc ccgcgattac
     1921 ctcgacggtg taattggacg acaggctcga ccagagcccg atctgcggcg tgcccgcgcg
     1981 cagcgcgcgc ttgaacgcgt tttccggcaa attcattcga acttcgcgcc cgactccttg
     2041 acgaccttgg cccacttcgc ctgctcggtg gtcatccact tgccgaggct ctcgggcgtg
     2101 tcggtgcgca cctcggtgcc ctgtttcgcg aacctgtcct tgatgtcttg tgtgttaagg
     2161 atttttctaa gctctccatt cagcttcgtc accgtctcgc gcggaatgcc aaccgggccg
     2221 accactccct ggtacgagcc tgtctcgaac cccggcaggc cctgctcggc gacggtcggc
     2281 gtatcggggg cgctcggcac gcgctgtgcg ctcgagatcg cgagcgcctt gagcctgccg
     2341 ccggtgacat ggggccatgt tgccagcatg ccgttgaaga ggacgtgcgc ctggccgccg
     2401 accacgccgg tgatggcgtc ggccccgcct ttgtacggga tataagtcca gtcgatgccc
     2461 atgcgctgcg caaactcgat tccggcgaga tgcggcgcgc cgccgatgcc cgacatggcg
     2521 aagttgagct tgccggggta cgccttcgcc atcgcgacga gctccttcac gtccttcact
     2581 ttcacctcgg gatggacggc gagcacgtgc ggcgaatacg acaccatcac gaccggcgcg
     2641 aagtccttcg acggattgaa cggcagcttc gggtagacgc tcgcgctgat caccaggccg
     2701 ccgaggtccg tgagaaggag tgtgtagccg tcgggcgcgc tcttcgcgac gaaatcggcg
     2761 ccgacattgc cgttcgcccc gggcttgttc tcgacgacca ccggctgccc ccaggctttg
     2821 ttgagctcag gcccgatctg ccgcgccaga atgtccgaag tgccgccggc cgcatagggc
     2881 acaacgatgc ggatcggctt cgtgggatag ctctgcgcgg atgcgcccgc ggcgcaaagc
     2941 gcgagcgtca gaaaggcgat gcgtttcatc cttgcctcct cagtggattt ctggttccgg
     3001 gaccggcgcc gtgccttcga ggaaatcgaa gtcgcagccc tcgtcggcct gcttcacgtg
     3061 gcgcgagaaa atcgcgccgt agcttcgctc gaacttcttg ctgggttgac gccatgccgc
     3121 gcggcgtttc ttcatttctt cttccgaaat ccgcaggttc agctcgcgct tctcgacgtc
     3181 gagctcgatt ggatcgccat ttttcaccag cgcgagcggg ccgccgacga acgactcggg
     3241 cgcgacgtgc agcacgcagc agccgtagct cgtgccgctc atgcg
//
LOCUS       DASTPD010000131         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_274164, whole genome shotgun sequence.
ACCESSION   DASTPD010000131 DASTPD010000000
VERSION     DASTPD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274164"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFR57_04600"
     CDS             <1..1060
                     /locus_tag="VFR57_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0258686.1"
                     /translation="VILSRAAKVAGGPTVTSRFVQRIAALAGKRWEGVVARGKRYLAL
                     ARALDTPAEVRHAERPAPKPPRAARPSKLSVTAIEDWLRDPYTIYARYVLRLQPLDAV
                     DTPPGARDRGTVIHGAIGDYTEMFANNPPADPLKELLKLGEHHFAPLNDYPEARAFWW
                     PRFERIARWFAAWDMDRRTGLTTLHAELRGELTFPAGTRAFTLSGIADRIELRKDGTY
                     AIIDYKTGQARTEKQVRTGLAPQLTLESAILRGGGFKPIAGGSVSEIAYVTLRGGAEP
                     GNPHKIEFKDGSPPDAHADRALAKLKELAAIFDDETKPYLSLVHPMWKTHYGDYDHLA
                     RVKEWSLAGGVEEDLPFS"
     gene            1057..>2273
                     /locus_tag="VFR57_04605"
     CDS             1057..>2273
                     /locus_tag="VFR57_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0258687.1"
                     /translation="MSAARTIPDHVRGVQIDAANPEVSAFVSANAGSGKTYVLAQRVI
                     NLLLRGVDPAKILCITFTKTAAANMANEVFKRLAAWTALDDAALDEQIRLSTGKTPSA
                     GERARARRLFASALETPGGLKVQTIHAFCTRLLHQFPFEANVAARFTVLDEPSTAQLL
                     NKLTLEVLLQGAGEPEGPLGKALGTAIVVAADITFKEVIGDAIKARDAIGAWIARAGN
                     IGQAIAQLSKTFGIAPDDSLEQVEQDFFSASLIPERDWPALIEILSKGAKSDKNNIDA
                     LIAARAASGRDRIDHYLRIFCTAELSPRKSLLTRALADRNPEWLERLLAEQERICALL
                     ERERALNARERTRALVTIAKEVIDRYQTEKERRALLDYDDLIDRTLALFKNVSAAWVL
                     YKLDLGIDHILID"
BASE COUNT          432 a          777 c          686 g          378 t
ORIGIN      
        1 agtcatcctt tcgcgcgcgg ccaaggtggc cggcggcccg accgtcacct ctcgctttgt
       61 gcaacgaatt gccgcgctcg ccggcaaacg ctgggaaggt gtcgtcgcgc gcggtaaacg
      121 ctacctcgcc ctcgcgcgcg cgctcgatac gccggccgag gtgaggcacg ccgagcgtcc
      181 ggcgcccaag ccgccgcgcg ccgcgcggcc cagcaagctg tcggtcaccg ccatcgagga
      241 ttggctgcgc gatccctaca cgatctatgc ccgctacgtt ttgcgcctac agccgctcga
      301 tgcggtcgat accccgcccg gcgcacgcga ccgcggcacc gtcatccacg gcgccatcgg
      361 cgactacacg gaaatgttcg ccaacaaccc gccggccgac ccgctgaagg aactgctcaa
      421 gctcggcgaa caccatttcg cgccgctcaa cgactacccg gaggcgcgcg cgttctggtg
      481 gccacgcttc gagcgcatcg cgcgttggtt tgctgcctgg gacatggatc ggcgcaccgg
      541 cctcaccaca ctgcacgccg aactccgggg cgagttgact ttcccggccg gcacgcgcgc
      601 attcacgctc tctggcatcg ccgaccgtat cgaactgcgc aaggacggca cctacgccat
      661 catcgactac aaaactgggc aggcgcgcac tgaaaagcag gtgcgtacgg ggctcgcgcc
      721 gcaattgacg cttgagagcg caatcctgcg cggcggcggc ttcaagccga tcgccggcgg
      781 ctcggtctca gagattgctt acgtgacgct caggggcggt gctgagccag gcaacccgca
      841 caaaatcgaa ttcaaagacg gcagtccgcc ggatgcccac gctgaccgcg cgctcgccaa
      901 gctcaaggag ctggcagcga tcttcgacga cgagaccaag ccctatctct cgcttgtgca
      961 cccgatgtgg aagacgcatt acggcgacta cgatcacctc gcgcgtgtca aggaatggtc
     1021 gctcgccggc ggcgtggaag aggacctgcc cttttcatga gcgccgcccg caccatcccc
     1081 gatcatgtgc gcggcgtcca gatcgacgcc gccaatccgg aagtctccgc cttcgtctcg
     1141 gccaatgccg gttcaggcaa gacctatgtg ctggcgcagc gcgtgatcaa tctgctgctg
     1201 cgcggcgtcg atccggccaa gattctctgc ataaccttta ccaagaccgc ggccgccaac
     1261 atggcgaatg aggttttcaa gcggctggcg gcgtggaccg ctctcgacga tgccgcgctg
     1321 gacgaacaga tcaggctcag cacgggcaaa acaccgagcg ccggagaacg ggcgcgggca
     1381 cggcggctgt ttgcgtcggc gctcgagaca ccgggcggac tgaaggtgca gaccatccac
     1441 gccttctgca cgcgactgct gcaccagttc ccgttcgaag ccaatgtcgc cgcgcgtttt
     1501 accgtcctcg acgagccatc gaccgcgcaa ttgctcaata agttgaccct cgaagtcctg
     1561 ttgcaggggg ccggcgagcc ggaggggccg ctcggcaagg cgctcggcac cgccattgtc
     1621 gtagccgccg atatcacctt caaggaggtg atcggcgacg ccatcaaggc gcgcgacgca
     1681 atcggtgcct ggatcgcgcg agccggcaat atcggtcagg caatcgcgca attgtcgaag
     1741 acctttggga ttgcgcccga cgatagcctt gaacaagtcg agcaggactt tttctcagct
     1801 tctctcattc cggaacgcga ctggccggcg ctgattgaga ttctctcgaa aggcgcaaaa
     1861 tccgacaaga acaacatcga tgcgctcatc gccgcgcgcg cggcatccgg ccgcgaccgc
     1921 atcgatcact atctgcgcat cttctgcact gccgagcttt cccctcggaa aagcctgctg
     1981 accagggcgc ttgccgaccg gaatcccgaa tggcttgagc gattgctggc cgaacaggaa
     2041 cgtatctgcg cgctgcttga gcgcgagcgc gcgcttaatg cccgcgagcg gacgcgtgcg
     2101 ctcgtcacta tcgccaagga agtgatcgac cgctaccaga ccgaaaagga gcggcgcgca
     2161 ctgctggact acgatgacct gatcgaccgc acgctggcac tgttcaagaa cgtttcagcc
     2221 gcctgggtac tctacaagct cgacctcggc atcgaccaca tcctgatcga cga
//
LOCUS       DASTPD010000132         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1499736, whole genome shotgun sequence.
ACCESSION   DASTPD010000132 DASTPD010000000
VERSION     DASTPD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499736"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFR57_04610"
     CDS             <1..761
                     /locus_tag="VFR57_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258688.1"
                     /translation="FLVASALAGFAAALVGIVFGLPSVRIKGFYLAVATLAAQFFIEW
                     ALTKFGWFSNYNPSGVITAQAIEIFGYEFNTPRSKYLLTLTIVAALALAAKNLARTQT
                     GRYWMAVRDMDLAAEVTGIDIVRTKLVAFAVSSFYCGVAGALWAYAYLGAVEPQAFTL
                     DRSFQVLFMIIIGGVGTVLGAFLGAAFIVLLPIALNVGAHTLLPGVSTDLMSNLELVI
                     FGALIVFFLAVEPHGLARLWQIGKEKLRLWPFPH"
     gene            772..2085
                     /locus_tag="VFR57_04615"
     CDS             772..2085
                     /locus_tag="VFR57_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258689.1"
                     /translation="MKSTIALAVAAALVAGTAIAQPKEQFIPMLSYRVGPYAAGGTGF
                     FGGYIDYLDMLNRRDGGIGGVKLTWEECETEYKNDRGVECYERLKKKGPTGATVFAAL
                     STGITYSLIERATADKIPVISMGYGRADASDGRVFPYIFPLVTNYWSQNTAKLKFIGM
                     KEGGMDKLKGKKIVNLYHGSAYGKETIPILDEQAKKYGFTVTHIEVPHPGNEQQSQWL
                     QIRSIKPDWVILRGWGVMNPTALKAAQKVGYKTERIVGVWWSGAEEDVLPAGDAAKGY
                     IAAAFHPSGTEFPVVQEILKHVYAGGKRGNMEDKGRIGQQYFNRGVIQGILTAEAIRT
                     AQTKFGQRPLSGEEMRWGIENLKIDEKRLKELGAYNLMQPMKVSCFDHEGGGAVKFQQ
                     WDGKQWKVISDWIASDQSIVRPQIEKSAAQYAVEKKLAPRDCPKG"
     gene            2090..>2638
                     /locus_tag="VFR57_04620"
     CDS             2090..>2638
                     /locus_tag="VFR57_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0258690.1"
                     /translation="MNPLLRVNNIEVIYDHVILVLKGVSLEVREGGIVALLGANGAGK
                     TTALKAVSNLLGAERGEVTKGSIEFQGERVERLNPSELVRRGVIQVMEGRHCFPHLTV
                     DENLLTGAHTRSANRFEIKRDLDRVYSYFPRLKERRTSLAGYTSGGEQQMTAVGRALM
                     ARPKMILLDEPSMGLAPQIVHEI"
BASE COUNT          489 a          867 c          860 g          422 t
ORIGIN      
        1 cgttcctggt ggccagcgct ctggccgggt tcgccgcggc actagtcggc attgtgtttg
       61 gcttgccgag cgtgcgcatc aagggcttct acctcgcggt ggcgacgctc gcggcgcagt
      121 tcttcatcga gtgggcgctg accaagttcg gctggttctc gaactacaac ccctccggcg
      181 tgatcaccgc gcaggcgatc gagatcttcg gctacgagtt caacaccccg cgcagcaagt
      241 atctcctgac gctcaccatc gtcgccgcgc tcgcgctcgc ggcgaagaac ctggcgcgca
      301 cccagaccgg gcgctactgg atggcggtgc gcgacatgga cctcgccgcc gaggtgaccg
      361 gcatcgacat cgtgcgcacc aagctcgtag ccttcgcggt gagctcgttc tactgcggcg
      421 tcgccggcgc gctgtgggcc tacgcctacc tcggcgcggt cgagccgcag gcgttcacgc
      481 tcgaccgctc gttccaggtg ctgttcatga tcatcatcgg cggcgtcggc accgtgctcg
      541 gcgccttcct cggcgcggcg ttcatcgtgc tgctgccgat tgcgctcaac gttggcgcgc
      601 acacgctgct acccggcgtc agcaccgatc tcatgtccaa cctcgagctc gtgatcttcg
      661 gcgcgctcat cgtcttcttc cttgccgtcg agccccacgg cctcgcccgc ctgtggcaaa
      721 tcggcaagga aaaactgcgg ctatggccgt ttccacacta ggaggacagg tatgaaatcc
      781 acaatcgcac tggcggtcgc agccgcgctc gtcgccggta ccgcaatcgc gcagcccaag
      841 gagcagttca tccccatgct gtcgtaccgc gtcggcccgt acgccgcggg cggcaccggc
      901 ttcttcggcg gctacatcga ctatctcgac atgctcaacc ggcgcgacgg cggcatcggc
      961 ggcgtcaagc tgacgtggga ggaatgcgag accgagtaca agaacgaccg cggcgtggag
     1021 tgctacgagc gcctgaagaa gaagggcccc accggcgcca cggtgttcgc cgcgctctcc
     1081 accggcatca cctattcgct catcgagcgc gccaccgccg acaagatccc cgtgatctcc
     1141 atgggctatg gccgcgccga cgcctccgac gggcgcgtgt tcccctacat cttcccgctc
     1201 gtgaccaact actggagcca gaacaccgcc aagctcaagt tcatcggcat gaaggaaggc
     1261 ggcatggaca agctcaaggg caagaagatc gtgaacctgt atcacggctc ggcctacggc
     1321 aaggagacga tcccgatcct cgacgagcag gcgaagaagt acggcttcac ggtgacccac
     1381 atcgaggtcc cgcacccggg gaacgagcag cagtcgcagt ggctgcaaat ccgctcgatc
     1441 aagcccgact gggtcatcct gcgtggctgg ggcgtgatga atccgaccgc gctcaaagca
     1501 gcgcagaagg tcggctacaa gaccgagcgc atcgtcggcg tgtggtggag cggggcggag
     1561 gaggatgtgc tgccggcagg cgacgcggcg aagggctaca tcgccgcggc gttccatccc
     1621 tcgggcaccg agttcccggt cgtgcaggag atcctcaagc acgtctatgc cggcggcaag
     1681 cgcggcaaca tggaggacaa gggccgcatc ggccagcagt acttcaaccg cggcgtgatc
     1741 cagggaatct tgaccgccga ggccatccgc accgcgcaga cgaagttcgg ccagcgcccg
     1801 ctcagcggag aggagatgcg ctggggcatc gagaacctga agatcgacga gaagcggctg
     1861 aaggagctcg gcgcctacaa cctgatgcag ccgatgaagg tctcgtgctt tgaccacgaa
     1921 ggcggcggcg cggtcaagtt ccagcaatgg gacggaaagc agtggaaggt gatcagcgac
     1981 tggatcgcga gcgaccagtc gatcgtgcgg ccgcagatcg agaagtcggc ggcgcaatat
     2041 gcggtggaga agaagctcgc gccgcgcgat tgcccgaagg gctaagccgg tgaaccccct
     2101 gctgcgcgtc aacaacatcg aggtcatcta cgaccacgtg atcctcgtgc tgaagggggt
     2161 atcgctcgag gtgcgggagg gcggcatcgt cgccctcctc ggcgccaacg gcgccggcaa
     2221 gaccaccgcg ttgaaggcgg tgtccaacct gctgggtgcc gagcgcggcg aggttaccaa
     2281 gggctcaatc gagttccagg gcgagcgcgt cgagcggctc aacccttcgg agctcgtgcg
     2341 ccgcggcgtg atccaggtga tggaaggccg tcactgcttc ccgcacctca cggtggacga
     2401 aaacctgctc accggcgcgc acacgcgcag cgcgaaccgc ttcgagatca agcgcgatct
     2461 cgaccgggta tacagctatt tcccgcggct caaggaacgg cgcacgagcc tcgccggcta
     2521 tacctcgggc ggcgagcagc agatgaccgc ggtcggacgc gcgctgatgg cgcggccgaa
     2581 gatgatcctg ctcgatgagc cttcgatggg actcgcaccg cagatcgtgc acgagatc
//
LOCUS       DASTPD010000133         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_682964, whole genome shotgun sequence.
ACCESSION   DASTPD010000133 DASTPD010000000
VERSION     DASTPD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682964"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VFR57_04625"
     CDS             complement(<1..164)
                     /locus_tag="VFR57_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU0258691.1"
                     /translation="MAYATIAGLPVQVGLYTAIVPMVVYAFLGSSRPLSVSTTTTIAI
                     LTGAELARVAP"
     gene            complement(227..526)
                     /locus_tag="VFR57_04630"
     CDS             complement(227..526)
                     /locus_tag="VFR57_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU0258692.1"
                     /translation="MRRVFFLELWNGLKLVWPIVSGLLAGMVLLGMVIAWREGWHFGD
                     GLYFAFVSGLTIGYGDLVPKHPMSRTLAIAIGFTGILLTGLVAAIAVRALERASK"
     gene            complement(528..2036)
                     /locus_tag="VFR57_04635"
     CDS             complement(528..2036)
                     /locus_tag="VFR57_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0258693.1"
                     /translation="MSRVLAFSISLLAVFVLAACSRGPDEAALKSEVQGRIDKIFKPG
                     LLELAALKRQGSSPLPAAQSSAERVLVYFNATLRLREGYDFKDWEGLSPATLAQVLGG
                     KERGVFGVKARENQPGDLLRVYGSGVYERSAQGWAAVAAGPGDAPRAPADPGNAAAST
                     SSKRYLDQLAAEIDIGPQGMDAESDQIVSEELQHALRAIQRRRARAHELLTFVSGPGD
                     GEYARVVEAILGTLHRRARRINVIGVETEGSIENGLLLGRGQANYGILQSDVAWLAAS
                     GVGPFAAEGPLAKIAALGSLYPEPVHVVVSAGSPIRRVEDLRGKRVDVGTPQSGSRLN
                     AISLLQAHGIALKDLAEARGEGTRAAIAQLRAGRIDAFVMTVGAPARVLQRLAAEHPI
                     RLVPVSTTAAARVVSEQPGLVRLTLPANTYPGQDQPVATVAPTALLAATVDTPNDEVK
                     ALLELTFERTDYLAFGSAQGVRIARATGLRGVAIPLHPAAAAYFGAPAPARK"
     gene            complement(2033..3244)
                     /gene="gltS"
                     /locus_tag="VFR57_04640"
     CDS             complement(2033..3244)
                     /gene="gltS"
                     /locus_tag="VFR57_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEU0258694.1"
                     /translation="MQTIELDLIHTILVSMIVLFVGRALVAKIEALQRFSIPAPVVGG
                     GLVAIALAFADGAAGLKFSFNMALKDSLLLMFFTTIGLAADARMLKKGGIKLIVFLAI
                     SATLIIAQNVVGIGAALALDLHPVIGMFAGSITLVGGHGTGAAYAGRLGETMNLQGVM
                     ELAMAAATAGLVIGAILGGPITEYLIKRHKLSGPAEGAIEDEAVQETQTDEVSSLSRL
                     NTLFVVLASLAGGKALVRLVEGTGFILPDFVFCLLLGVLIRNSTIFVKSLRISDRTVE
                     TVGDVTLSLFLVMALMTMKLLDLANLAGPLLVILAAQTAVMLVFTVFVTYNAMGRNYD
                     AAVISGGQVGFALSSTACALAIMRAVTQRHGASPIAFLIVPMVGAFFIDIVNALAIQG
                     TLALPLFGFGP"
     gene            complement(3331..>4254)
                     /locus_tag="VFR57_04645"
     CDS             complement(3331..>4254)
                     /locus_tag="VFR57_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU0258695.1"
                     /translation="RVLGRPDRRLVASGKGVASFNGKRFGVKGELRADSGNFEIGSDS
                     LPTIDDDVEVEGAPQGAVRPVRKQGPLPLDLDLKLDLGSRLTLRAYGFKGGVTGQLRV
                     YTGPAGELFAQGRVEAVKATFYAYGQELEVDPGSVIFDGPIGNPALEITAWRRHQQVE
                     AGLRVTGTVQTPRVQLVSNPAVGENEKLSWLVLGRAPAEAVGADLALLQAAGGAVFGR
                     GGDEPINRRFAKRFGFDELTVRGSSQLEGNVVALGKRYSDDIYISFEQAISTTTEYLV
                     KLDYALTQRISLRGQTGTTSGVGVFYRYSWD"
BASE COUNT          727 a         1460 c         1417 g          650 t
ORIGIN      
        1 ggcgcgacgc gggcgagttc ggcaccggtg aggatggcga tggtcgtcgt cgtgctcacg
       61 ctcaaggggc gcgagctgcc gagaaatgca tacaccacca tcggcacgat ggcggtgtag
      121 agcccaacct gcaccggcag cccggcgatc gtggcgtagg ccatcgcctt ggggaccacg
      181 accgccgccg cggtcaggcc ggcgaagaga tcggctttga acacgtctac ttcgacgcgc
      241 gctccaatgc gcgcacggcg atcgccgcga ccaggccggt gagcaggatg ccggtgaagc
      301 cgatcgcgat cgcgagcgtg cgcgacatgg ggtgcttcgg caccaggtcg ccgtagccga
      361 tcgtcagccc cgacacgaac gcgaagtaaa gcccgtcgcc gaagtgccag ccctcgcgcc
      421 aggcaatcac catgccgagg agcaccatgc cggcaagcag gccggacacg atcggccaca
      481 ccagcttgag gccgttccac agctcgagga agaagacccg tctcacgcta tttcctggct
      541 ggcgccggcg cgccgaaata ggccgccgcc gcggggtgca aggggatggc cacgccacgc
      601 aggccggtgg ccctggcgat ccttacgccc tgtgcactgc cgaacgccag gtagtcggtg
      661 cgctcgaacg tcagctcgag cagtgccttc acctcgtcat tcggcgtgtc aacggtggcg
      721 gcgagcaacg cggtgggcgc cacggtagcg accggctggt cctgaccggg ataggtgttc
      781 gctggaaggg tgaggcgcac cagaccaggc tgctcgctta ccacgcgggc agcggccgtg
      841 gtcgagaccg gcaccaggcg gatcgggtgc tcggccgcca gccgctgcaa cacgcgcgcc
      901 ggcgcgccta cggtcattac gaaagcgtcg atgcggccag cgcgcagctg cgcgatcgcg
      961 gcccgcgtgc cttcgccgcg cgcctcggcg aggtccttga gcgcaatgcc gtgggcctga
     1021 agcaggctga tcgcgttcag tcggctgccc gattgcggcg tgccaacgtc cacgcgcttg
     1081 ccgcgcaagt cctcgaccct gcggatgggc gagcctgccg agacgaccac atgcaccggc
     1141 tccgggtaga ggctaccgag cgcggcaatc ttggcgagcg ggccctcggc cgcgaacggc
     1201 cccacccccg aggccgcgag ccatgcgacg tcgctctgca gaatgccgta gtttgcctgc
     1261 ccgcgcccca ggagcagccc gttctcgata ctgccctcgg tttcgacgcc gatcacgttg
     1321 atcctgcgtg cgcgcctgtg tagggtgccg aggattgctt ctaccacacg cgcgtattcg
     1381 ccgtcgccgg ggccgctgac gaacgtaagc agctcatgcg cgcgcgcgcg ccggcgctgg
     1441 atcgcgcgca gcgcgtgctg gagctcctcc gaaacgatct ggtcggactc ggcatccatg
     1501 ccctgcggcc ctatgtcgat ctcggccgca agctggtcga gatagcgttt cgagctggtc
     1561 gaggccgccg cattgccggg atccgcagga gcccgcggcg catcgcccgg ccccgcggcg
     1621 accgcggccc agccttgcgc cgagcgctcg tagaccccgc tgccgtagac ccgcagcagg
     1681 tcgcccggct ggttctctct tgccttgacg ccgaatacgc cgcgctcctt tccgccgagc
     1741 acctgcgcaa gcgtcgccgg actcaggcct tcccagtcct tgaagtcata gccttcgcgc
     1801 aggcgcagcg tggcgttgaa gtagacgagg acgcgctcgg cgctgctctg cgccgccggc
     1861 agcgggctcg agccctggcg cttgagcgcc gcgagctcga gcagcccggg cttgaagatc
     1921 ttgtcgatgc ggccctggac ctcgctcttg agcgcggcct cgtccggccc ccgggaacag
     1981 gcagccagaa caaagactgc aagaagactt atcgaaaaag cgagaacccg gctcatggcc
     2041 cgaatccgaa cagcggcagc gcgagcgtgc cctggatcgc gagcgcgttc acgatgtcga
     2101 tgaagaacgc gccaaccatc ggtacgatca ggaacgcgat cggcgacgcg ccgtgacgct
     2161 gcgtcaccgc ccgcatgatc gcgagcgcgc acgcggtcga agacagcgcg aagcccacct
     2221 gcccgccgct gataaccgcg gcgtcgtagt tgcgccccat cgcgttgtac gtcacgaaca
     2281 ccgtgaacac gagcatcacc gcggtctgcg cggccaagat cacgagcagc gggccggcga
     2341 ggttggcgag atcgagcagc ttcatcgtca tgagcgccat cacgaggaac agcgacagcg
     2401 tcacgtcgcc caccgtctca acggtgcggt cgctgatccg cagcgatttg acgaagatgg
     2461 tggagttgcg gatcaggacg ccgagcagca ggcagaacac gaaatccggc aggatgaacc
     2521 cggttccttc gaccagccgt accagcgcct ttccgcccgc aaggctggca agcacgacga
     2581 acagcgtatt cagccggctg agcgagctga cctcgtcggt ctgcgtttcc tggacagctt
     2641 catcttcgat tgcgccttcc gcgggcccgg acagcttgtg ccgcttgatc aggtactcgg
     2701 tgatcgggcc gccgagaatc gcgccgatga cgagcccggc cgtggccgcg gccatggcga
     2761 gctccatcac gccctgcagg ttcatcgtct cgcccaggcg ccccgcgtac gccgcacccg
     2821 tgccatgccc gccaacgagc gtgatcgagc cggcaaacat accgatcacc gggtgcaggt
     2881 cgagggcgag cgcagcgccg attccaacga cgttctgcgc gatgatcagg gtggcgctga
     2941 tcgccaggaa gacgatgagc ttgatgccgc cctttttcag catgcgcgcg tcggcggcga
     3001 ggccgatggt ggtgaagaac atgagcagca gcgagtcctt cagcgccatg ttgaaggaga
     3061 atttcaggcc ggcggccccg tcggcgaagg cgagcgcgat cgccaccagg ccaccgccca
     3121 cgacgggggc ggggatgctg aaccgctgca gggcttcgat cttcgccacc agcgcgcggc
     3181 cgacaaagag cacgatcatg gacacgagga tcgtgtgaat gaggtccagc tcgatcgtct
     3241 gcactgactg ctggctcttg gtctttcggc gccagcgacc ttagggcagc gaagccgccc
     3301 gcgaaatcgg accaaggtac tactgagcgc tcagtcccag gagtaccggt agaaaacgcc
     3361 gacgccgctc gtggtgccgg tctggccgcg cagcgagatt cgctgagtta gcgcatagtc
     3421 cagcttcacc agatactcgg tggtcgtgct gatcgcctgc tcgaagctga tgtagatgtc
     3481 gtcggaataa cgcttgccga gcgcgaccac gttgccttcg agctggctcg agccgcgtac
     3541 tgtaagctcg tcgaagccaa agcgcttcgc gaagcgacga ttgatcggct catcgccgcc
     3601 gcgcccgaac accgcaccgc ccgccgcctg cagcagcgcg aggtccgcgc ctacggcttc
     3661 cgccggcgcg cgcccgagca ccagccagga gagtttttcg ttctcgccca ccgccggatt
     3721 ggagaccagc tgtacgcggg gagtctgcac tgtgccggtc acgcgcaagc ccgcttccac
     3781 ctgctggtgc ctgcgccagg cggtgatctc gagcgcggga ttgccgatcg ggccgtcgaa
     3841 gatgaccgag cccggatcga cttcgagctc ctggccgtag gcatagaaag tggctttgac
     3901 cgcctccacg cggccttgcg cgaaaagctc gcccgccgga ccggtgtaga cgcgcagctg
     3961 accggtgaca ccgcctttga agccgtaggc gcgcagcgtc agacggctgc ccagatcgag
     4021 cttgaggtcg aggtcgaggg gcagcggacc ctgcttgcgc accgggcgca ccgcgccctg
     4081 gggtgcgcct tccacctcga cgtcgtcgtc gatcgtcggc agggaatcgg agccgatctc
     4141 gaagttgccg ctgtccgcgc gcagctcgcc cttcacgccg aagcgcttgc cgttgaagct
     4201 tgccacgccc ttccccgagg ccaccaggcg ccggtcgggc ctgccgagga cgcg
//
LOCUS       DASTPD010000134        17289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_70351, whole genome shotgun sequence.
ACCESSION   DASTPD010000134 DASTPD010000000
VERSION     DASTPD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70351"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFR57_04650"
     CDS             complement(<1..264)
                     /locus_tag="VFR57_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU0258696.1"
                     /translation="MIIGTGFSGLLAAIGLQKRHCNDFVLLERNAELGGTWQVNSYPG
                     LEVDVPTSLYCISFIPYPFRKSFSPQSELLAYTNHIIDRFGLRK"
     gene            complement(734..841)
                     /locus_tag="VFR57_04655"
     CDS             complement(734..841)
                     /locus_tag="VFR57_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258697.1"
                     /translation="MAPERRDEIVALGLRSIVAGTLATLMVGALVGMLT"
     gene            complement(859..1065)
                     /locus_tag="VFR57_04660"
     CDS             complement(859..1065)
                     /locus_tag="VFR57_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU0258698.1"
                     /translation="MAPLVWLAGVPAAEAATAGALMGTKTVLNEFIAYVDMAQLPADA
                     LSARSRLIMTTACAASPTSAASAS"
     gene            1113..1271
                     /locus_tag="VFR57_04665"
     CDS             1113..1271
                     /locus_tag="VFR57_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258699.1"
                     /translation="MGVFEAIAYVDFNAGGDMMKFAGSVALGRGIRMRLFSSLAEAEK
                     WLENPWPR"
     gene            complement(1272..1655)
                     /gene="gloA"
                     /locus_tag="VFR57_04670"
     CDS             complement(1272..1655)
                     /gene="gloA"
                     /locus_tag="VFR57_04670"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEU0258700.1"
                     /translation="MRMLHTMLRVGDMQRSVNFYTEVLGMKLLRTTDRPEQKYSLAFV
                     GYDDEERAAVLELTYNYGVDRYDLGTAFGHIAIGVPDVKAACERVRRSGGKVTREPGP
                     VKGGTTVIAFVEDPDGYKIEFIERS"
     gene            complement(1652..2353)
                     /locus_tag="VFR57_04675"
     CDS             complement(1652..2353)
                     /locus_tag="VFR57_04675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEU0258701.1"
                     /translation="MDRRTAKSSHLIRLGGREVEYRFVRRRRRTLGITVDAGGLKVSA
                     PLRAPWRDIEGFVREKERWILSKLDEWARAPRPTLVRSATGERLPLFGTSVRLERCGG
                     PARREDDALRFGSMPQLVQWLKATALCALRPRVAHFAARVGRPAPRVALSNARTQWGA
                     CNEGGGIRLSWRLVHVEPALADYVVAHEAAHLVELNHSERFYRVLASLYPDWLAARER
                     LELAGAALPVLKETR"
     gene            complement(2320..3015)
                     /locus_tag="VFR57_04680"
     CDS             complement(2320..3015)
                     /locus_tag="VFR57_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0258702.1"
                     /translation="MRSVLFALGLVLITLPYALIALATFPLPRMARYRIISGWSRLVV
                     LMARTIVRIDWRVEGRENLPSRPSVILAKHQSAWETMAFQLIFPPQVLVLKRELLWIP
                     FFGWGLALMSPIAIDRSRGVAALRAIARRGRERLRQGFWVVVFPEGTRVSPGASRAYH
                     PGGAWLACAAGAPVVPVAHNAGLFWPRNAFLKRPGTVTVRIGPPIDAANRDAETVNEL
                     AKTWIEEQQKALT"
     gene            complement(3021..3554)
                     /gene="gmhB"
                     /locus_tag="VFR57_04685"
     CDS             complement(3021..3554)
                     /gene="gmhB"
                     /locus_tag="VFR57_04685"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEU0258703.1"
                     /translation="MKLVILDRDGTVNEDSDDYIKSPEEWRPIKGSLEAIARLTQADY
                     RVVVATNQSGIARGLFDTRTLFAIHDTMLRALAQHGGRIDAFFFCPHAPEAGCHCRKP
                     QPGMLLEVARRFNVPLDQAYMVGDTQRDLEAAAAAGARPVLVLTGKGAQARDAGKLPA
                     GTRVFPDLAAFAAHLAP"
     gene            complement(3551..5605)
                     /gene="glyS"
                     /locus_tag="VFR57_04690"
     CDS             complement(3551..5605)
                     /gene="glyS"
                     /locus_tag="VFR57_04690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU0258704.1"
                     /translation="MALTLLVELLTEELPPKSLRALANAFSSALVAALKGEGFLSGDS
                     AATAFATPRRLAVSITDVHEKSPDRQLQLTGPSVNAPPQAVAGFAKKNGVSVDDLTKL
                     DTPKGEVFAYRRIAAGSHLAANLALRVEEALKRLPVAKLMRWGSGDAQFVRPVHGLVM
                     MHGRQVVPGRVLDLESTNRTQGHRFMGKGTITLADATKYETTLREEGMVIADFAARRD
                     EIERQLKAEAARERGALGAYEDLLDEVTALVEHPSVYVGSFDAAFLAVPQECLILTMR
                     QNQKYFPLFDGAGKLVPRFLIVSNMKLADPQHIIAGNERVVRPRFEDARFFYEQDRKV
                     RLEARVPELAKVIFHNKLGSQLDRVMRVQMLSGHIARSLSADVAAAERAAWLSKADLL
                     TGMVGEFPELQGVMGRYYALNDGEPRPVADAIQGHYRPRFAGDRLPDDEVACAVALAD
                     KLDALAGMFGIGQQPTGEKDPFGLRRAALGVVRVLVERKLGLSWHALVDSAFNTYASK
                     VKDARAEMHAFVLERFAGYLKDQGFSTLQVDAVLSQNPARLDLVPQQLEAVRAFQELP
                     EAESLAAANKRVANILRQAAAQGESFKPVERKALKEPAELALFTALEMTRGNALPMFE
                     RGDYTGYLKSFAVLKKPVDAFFDSVMVMVDDAELRQGRLALLNDLRDAMNRVADISKL
                     AQ"
     gene            complement(5606..6484)
                     /gene="glyQ"
                     /locus_tag="VFR57_04695"
     CDS             complement(5606..6484)
                     /gene="glyQ"
                     /locus_tag="VFR57_04695"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0258705.1"
                     /translation="MPTFQQLIGQLNAYWDRQGCALLQPYDMEVGAGTFHTATFLRAI
                     GPEPWSAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPVEIQDKYLGSLKALGI
                     DPAQHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQEVGGLACKPVLGEITY
                     GLERLSMYLQGKDSVFDLIWTPGVAYRDVYHQNEVEQSKYNFELSNADMLFRHFGDFE
                     AEAKRLIAARCVLPAYEMVMKASHTFNLLDARGAISVTERQAYIGRVRTLAKEVAHGY
                     YESRERLGFPMLSKAT"
     gene            complement(6494..7936)
                     /gene="lnt"
                     /locus_tag="VFR57_04700"
     CDS             complement(6494..7936)
                     /gene="lnt"
                     /locus_tag="VFR57_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU0258706.1"
                     /translation="MLYSVLAGAATVLAFAPFGLHPFALLTLALLIHLWMRAPPQRCA
                     LLGFAFGAGLFGAGVSWVYVSLHQFGGMAAPLAVFATLVFCALLALFPAVAGWLQARV
                     PASDAARAVLLIPATWVLCEWMLSWIFTGFPWLALGYSAAGWPLQGYAPLGGVYMVSF
                     VIVSAAGMLWLLAGQKPRFLVVLLLVVGTGEALRHVAWTVPAGAPVPTALLQGNIEQE
                     MKFHPERAPRILETYARLAEGTQARLVILPETALPAFLDRIDPAYLARLDAVGKRNEG
                     DLLIGVPYRTGRDAYYNSALSLGVSPRQIYHKVHLVPFGEFVPPGFRWTLDVLSIPLS
                     DFSRGSFDQPPLEVAGQRVAVNICYENVFGAEIARSLPEATLLVNMSNVAWFGDSLAP
                     AQHLQIARLRAIETGRMHLTATNTGITAAIDRDGRVLARLEQFTEGRLEIPAQGYAGA
                     TPYVRLRDWPVVVACLLIFALFTVRAKRSR"
     gene            complement(7951..8775)
                     /locus_tag="VFR57_04705"
     CDS             complement(7951..8775)
                     /locus_tag="VFR57_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEU0258707.1"
                     /translation="MNDQSRPSLLERLSALLMREPGDRSELTQLLRSAYSRNLLDADA
                     LSIIEGALTVSEMAVRDIMVPRAQVDFIDINEPVEKFVKQVIATAHSRFPVIDQNRDD
                     VIGVLLAKDLLRFYAGEEEFNVREMLRPAVFVPEAKRLNVLLREFRASRNHMAIVIDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEASDNILPEPGGRHRVKALTQIADFNAAFG
                     THLSDEAADTIGGYLIKHLGRLPKRGESVVMEGLRFQVLRADSRRVYTLLVDRLKS"
     gene            complement(8787..9164)
                     /gene="ybeY"
                     /locus_tag="VFR57_04710"
     CDS             complement(8787..9164)
                     /gene="ybeY"
                     /locus_tag="VFR57_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU0258708.1"
                     /translation="MPRRGVPSAASLRKWALAASRSPLTLRVVGEREGRALNKAFRGK
                     DRATNVLTFNTGDIALCHPVIRREARAQGKTVAAHYAHLVVHGCLHLHGYAHEKKRDA
                     ARMEAREIRILRSAGIANPYGVE"
     gene            complement(9184..10125)
                     /locus_tag="VFR57_04715"
     CDS             complement(9184..10125)
                     /locus_tag="VFR57_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0258709.1"
                     /translation="MRAKPVEVKLDPVDNQRLARLCGALDENIRQIESALDVTIARRG
                     AKFTVKGEQAQRAAEAIEHFYDRASGDLTVEDVQLGLTELSKKISLENEGPQLLTRRA
                     DLHGRTPHQVEYIESILAHDLTFGIGPAGTGKTYLAVACAVDALERDKVKRIVLVRPA
                     VEAGERLGFLPGDLAQKVDPYLRPLYDALFDLMGFEKTGKLLERGTIELAPLAYMRGR
                     TLNHAFIILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIEARRI
                     LTGVRGIAFTQFGAQDVVRHPLVARIIDAYDSRQQEE"
     gene            complement(10122..11438)
                     /gene="miaB"
                     /locus_tag="VFR57_04720"
     CDS             complement(10122..11438)
                     /gene="miaB"
                     /locus_tag="VFR57_04720"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU0258710.1"
                     /translation="MKKLYLRTFGCQMNEYDSDRIADVMREAEALELTPDPAQADVII
                     FNTCSVREKAQEKVFNDLGRVKHLKRQRPSVMIAVGGCVASQEGPAIVQRAPYVDVVF
                     GPQTLHRLPELLERRRASGQPQVDVSFPEIEKFDHLPPAKVEGASAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVIAELAGLAAQGVKEVTLLGQNVNAWRGDIAEAPADFAE
                     LLYYLDEIPELVRIRYTTSHPREFTQRLIDAHRDIDKLAPHVHLPVQSGSDRVLAAMK
                     RGYTALEYRSIVRRLRQARPGISLSSDFIVGFPGETEADFQATLKLARDADFDVSFSF
                     VYSPRPGTPAAELPGAVSHSEKIERLLRLQAQLEAQARAISSAMVGTTQRVLVEGHSK
                     KRKTELQGRTGNNRVVNFPGPESLIKQYVHLRIAAALPHSLRGELV"
     gene            complement(11435..11752)
                     /locus_tag="VFR57_04725"
     CDS             complement(11435..11752)
                     /locus_tag="VFR57_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258711.1"
                     /translation="MRVLVFAILASCAALAMAQLRTIPKEAVLGQLRHLEAMVVEIDG
                     KPRSLAPGVQIRDPDNRLVLPVSLVEKTQVRYLVDSTGMVHRVWILSDAEKAALPPAP
                     FPK"
     gene            complement(11752..12171)
                     /locus_tag="VFR57_04730"
     CDS             complement(11752..12171)
                     /locus_tag="VFR57_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0258712.1"
                     /translation="MDCTVRWTADGMSFIAETGSKHLVAMDGAPEGGGRNLAPRPMEL
                     VLAGSGGCTAYDVVVILKKSGQDVTGCEVKLSAERAATDPKVFTKVHMHFVVRGRTLK
                     RNLVEQAIRLSHEKYCSATAMLLKTAEVTRDFEIMDT"
     gene            12223..12651
                     /gene="rplM"
                     /locus_tag="VFR57_04735"
     CDS             12223..12651
                     /gene="rplM"
                     /locus_tag="VFR57_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0258713.1"
                     /translation="MTTFSAKTGEVQHGWYVVDAQDKVLGRLASQIAARLRGKHKPQF
                     TPHVDTGDYIVVVNAAKLRVTGRKPERKIYYRHSGRPGELHQINFAKLHAKRPERVLQ
                     IAVKGMLPKGPLGYAMMKKLKVYGGATHPHSAQQPKALEI"
     gene            12653..13045
                     /gene="rpsI"
                     /locus_tag="VFR57_04740"
     CDS             12653..13045
                     /gene="rpsI"
                     /locus_tag="VFR57_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0258714.1"
                     /translation="MVGDYYYGTGRRKSAVARVFLKPGKGEFKVNGKPVDQFFGRETG
                     RMVVRQPLELTKSLSSFDIMVNVYGGGESGQAGAVRHGIARALIEYDAGLKSALSTAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            13097..14137
                     /gene="argC"
                     /locus_tag="VFR57_04745"
     CDS             13097..14137
                     /gene="argC"
                     /locus_tag="VFR57_04745"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0258715.1"
                     /translation="MIRAGIVGGTGYTGVELLRLLAQHPDVELKAITSRKEAGTPVAR
                     MFPSLRRRVELAFSEPSARALAGCDVVFFATPNCVAMSEARAVLEGGARIVDLSADFR
                     IHDVAQWERWYKTKHAAPELIREAVYGLPEMNREQLRRARLVANPGCYPTSIQLGFLP
                     LVEAGVVDVEHLIADAKSGASGAGRKTELNLMFSEASDNFAAYHITGHRHWPEIRQGL
                     AQAAKRDVGLVFTPHLTPLIRGIHATLYARITREADFQQLFEKRYAGEPFVDVMPAGS
                     HPDTRSVRAANVCRLAVHRPPESAGRSDTIVVLSVIDNLMKGASGQAVQNMNLMFSLP
                     ETAGLEHVAVVP"
     gene            14138..14893
                     /locus_tag="VFR57_04750"
     CDS             14138..14893
                     /locus_tag="VFR57_04750"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEU0258716.1"
                     /translation="MPSGRWKVIRSRFGIAAPRVEIHTQIPWYLRWVGIAVLLGLAAI
                     AASWIYDAGGRFAGFDRSEVLQELQRAQGELDNAREELERLRAMANAADSRVSIERTA
                     QQKLAQQVRALEQENARVREELAMVEQMLTSEERHTQTLAIYQFKVQPDVLPGEYRYR
                     LLLLTPTDRRGRDFSGRLELVVNLHEGGQNAMMSFPEPGDAAAAAFRLAFKYFRRVEG
                     TFRVSPKAKVESVQVRIYETGSSQPRATQSVTL"
     gene            14920..15318
                     /locus_tag="VFR57_04755"
     CDS             14920..15318
                     /locus_tag="VFR57_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0258717.1"
                     /translation="MFNKTSKPQNRIDSLIGATTRIEGNVIFSGGLRVDGMVRGNIAA
                     LPDQPGTLVVSSDGRIDGEVQAAHIVVSGTIQGPVHATETLELQAGSRVKGDVYYKSI
                     EVHQGAVVEGRLVPHPAEAKGVELKLASGG"
     gene            15337..15687
                     /gene="erpA"
                     /locus_tag="VFR57_04760"
     CDS             15337..15687
                     /gene="erpA"
                     /locus_tag="VFR57_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU0258718.1"
                     /translation="MNAVTEMPAPLVFTDNAANKVKQLIEEEGNPELKLRVFVTGGGC
                     SGFQYGFTFDEVQNDEDASMEKNGVTLLIDPMSYQYLVGAEIDYQESLEGAQFVIKNP
                     NAQSTCGCGSSFST"
     gene            complement(15735..16973)
                     /locus_tag="VFR57_04765"
     CDS             complement(15735..16973)
                     /locus_tag="VFR57_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0258719.1"
                     /translation="MRTLGRLGGIAAIGLLAASGVVAFATIAPRAEERVQPPRRPIVE
                     ALEIRADATPAPTSYFREELFQRGDTLPAFLERLGIEAPHAARLTKLRVLQQLRPGTH
                     VSAEVSAEGEPLVLSFLSGRDTVVKVAAEANGQFRVTDDRAQLYTRVAMKSGVIESSL
                     FAAADDAGIPDSIAMQLGEVFGGDIDFHRDLRKGDQFAVVYELHYLGGRPVRAGRVLA
                     AEFTNNRKTFRAVHFRTGYFTPEGKNLRKAFLRSPLEFSRVSSGFGMRRHPIAQAWIK
                     HQGIDYAAPSGTRVRAVGDGVVEHAGLKGGYGKVVILRHHGQYSTIYAHLSRINVHRG
                     QRVAQNDTIGAVGQTGWATGPHLHYEFRVAGEARNPLSVAMPAALPVTPQDLAAFRSH
                     AEPLITRLDLIANSALARLD"
     gene            17047..>17289
                     /locus_tag="VFR57_04770"
     CDS             17047..>17289
                     /locus_tag="VFR57_04770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869823.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU0258720.1"
                     /translation="MSAIRQAIQTIRRGAEELIVEEELKNKLASGRKLRIKLGLDPTA
                     PDLHLGHTVVINKVRDFQNLGHQVQFLIGDFTGMIGD"
BASE COUNT         3106 a         5507 c         5723 g         2953 t
ORIGIN      
        1 ctttcgcaag ccgaacctgt cgataatatg gttcgtgtat gcgagcaatt cgctttgcgg
       61 ggaaaaggat ttcctgaacg gatagggtat gaacgagatg cagtaaagac tggttggaac
      121 gtccacctct aggccgggat atgagttgac ctgccaggtt ccgcccaact cggcgtttct
      181 ttccaagagg acaaagtcgt tgcagtgcct tttctgcaga ccaatggcag ccaatagccc
      241 tgagaatccg gtcccgatga tcacggtctc ggagtaccga ggctgccttg ggctgctggc
      301 gaacgccggc ctctcgtcgg tggctggatg cgctgatgca ctcatcgtcg aatccttgta
      361 tcgccccgtg gatcgcggga gtccgatttg tttgagtcag gctggcccgg ccgactcccc
      421 gggccgacac gaacgcgacc tgtttcaacg agcgatagga aaatcaatcc acctgcgatt
      481 ccgacgcgcc gaatctgcgc gccgggagtc agggcaagag gagcaaagcc ccggtcaaca
      541 tggccgggga tgctagtcag cgaaaattag cggagcatta actgcgccgg gcatcccgtg
      601 acggtgtcac gatcgccgat gcaaattccc gtcctcggga atcgactatt gcactatgcc
      661 gcgtagtggg ttcccagccg tggattcagc acgtcccgga gccgatcccc gaccaggttg
      721 ttcgcgacat tgctcaagtg agcatgccca ccaacgctcc gaccatcagc gtcgcgaggg
      781 tgcccgcgac gatggagcgc agcccgagcg cgacgatctc gtcgcgccgt tccggcgcca
      841 tggcggccat cccgccaatc aggatgccga ggctgccgaa gttggcgaag ccgcacaggc
      901 agtggtcatg atgagccggc tccgcgcgct cagcgcatca gccggaagct gcgccatgtc
      961 cacgtaggcg atgaattcgt tgagcacggt cttcgttccc atcagcgccc cggccgtggc
     1021 ggcttccgcc gcgggcacgc cggcgagcca gacgagcggc gccatcgcgt agcagcgtga
     1081 tctgcgcccc gccccagtga aggataggaa atttgggggt gttcgaggcg atcgcctacg
     1141 tggacttcaa cgccggcggc gacatgatga agttcgccgg gagcgtcgcg ctcggccgcg
     1201 gcattcggat gcgactcttc agcagcctgg cggaggccga gaagtggctc gagaacccct
     1261 ggccgcgttg actagcttcg ctcgatgaac tcgatcttgt agccgtcggg gtcctcgacg
     1321 aaggcgatca cggtcgtgcc gcccttcact ggaccgggct cgcgtgtgac cttgccgccc
     1381 gagcgcctta cgcgctcgca cgccgctttc acgtcgggaa caccgatcgc gatgtgtccg
     1441 aacgcggtgc ccaggtcgta gcggtccacg ccgtagttgt acgtaagctc cagcaccgcg
     1501 gcgcgctctt cgtcgtcgta gccgacgaag gcgagcgagt atttctgctc gggccggtcg
     1561 gtggtgcgca gaagtttcat gcccagcacc tcggtgtaga agttcaccga gcgctgcatg
     1621 tcgcctacgc gaagcatcgt atggagcatt ctcatcttgt ttccttcagg acaggtagag
     1681 cggctccggc aagctcgagg cgctcgcgcg ccgccagcca gtcgggatag agggaagcga
     1741 ggacgcggta gaagcgctcc gagtggttga gctccacgag atgcgcggct tcgtgtgcga
     1801 ccacatagtc cgctagggcg ggctccacgt ggacgagtcg ccaggatagc cggatgcccc
     1861 cgccctcgtt gcaagcgccc cattgagtgc gcgcattcga aagcgcgacg cgcggcgccg
     1921 gccgcccgac gcgggcggcg aaatgagcga cacgtgggcg caacgcgcaa agcgccgtcg
     1981 ctttcagcca ttgcacgagc tgcggcatgc tgccaaagcg cagggcgtcg tcctcgcggc
     2041 gcgccggacc gccgcagcgc tcaaggcgaa cgctcgtgcc gaaaagcggc agacgctcgc
     2101 cggtggcgct ccgcacgagc gtcggccgcg gcgcacgcgc ccattcgtcg agcttggaga
     2161 gaatccagcg ctccttctcc cgcacgaatc cctcgatgtc gcgccacggc gcgcgcagcg
     2221 gcgccgacac tttgagcccc ccggcgtcga ccgtgatgcc aagtgtgcgc cggcgccggc
     2281 gcacgaaccg gtactccacc tcgcgcccgc ccaggcggat caggtgagag ctttttgctg
     2341 ttcttcgatc catgtcttcg ccagctcatt gaccgtctcg gcgtcgcggt tggcagcgtc
     2401 gatcggcggt ccgatgcgca cggtgaccgt gccgggccgc ttcaggaagg cgttgcgcgg
     2461 ccagaagagt cccgcgttgt gcgccacggg gaccaccggc gcgcctgccg cgcacgcgag
     2521 ccaggcgccg ccgggatgat aggcgcgcga agcgccgggc gagacccgag ttccttcggg
     2581 aaagaccacc acccagaaac cctgccgcag ccgctcccga ccgcgccggg cgatcgcgcg
     2641 cagcgccgcc acgccgcgcg agcggtcgat cgcgatcggg ctcatcaggg cgagtcccca
     2701 gccgaagaac gggatccaga ggagctcgcg cttgagcacc agcacctgcg gcggaaagat
     2761 gagctggaac gccatggtct cccaagccga ttggtgcttg gcgaggatca cggaagggcg
     2821 cgagggcaga ttttcgcgcc cctcgacccg ccagtcgatg cgcacgatcg ttctcgccat
     2881 gagcacaacc aggcgcgacc agccggagat gatgcgatag cgcgccatgc gtggcagcgg
     2941 aaaggtggcg agcgcaatca gcgcgtacgg cagggtgatg agaacgagtc ccagcgcgaa
     3001 caacaccgag cgcacaaatc tcacggagca aggtgcgcgg cgaatgccgc gagatcggga
     3061 aacacgcggg ttccggccgg cagtttcccg gcgtcgcgcg cctgcgcgcc cttgccggtg
     3121 agcacgagca ctggccgcgc tccggccgcg gccgcggcct cgaggtcgcg ctgagtatcg
     3181 cccaccatgt aggcctggtc gagcggaaca ttgaagcggc gcgccacctc gagcagcatg
     3241 cccggctgcg gcttgcggca atggcagccc gcttccggcg cgtgcgggca gaagaaaaac
     3301 gcgtcgatgc gtccgccgtg ctgggcgagc gcacgcagca tcgtgtcgtg gatcgcgaag
     3361 agcgtgcgtg tgtcgaacag cccgcgcgcg attcccgact ggttcgtcgc gacgacgacc
     3421 cggtagtcgg cctgtgtgag ccgcgcgatc gcttccaggc tacccttgat cgggcgccac
     3481 tcctcgggtg acttgatata gtcgtcgctg tcttcgttga ccgtaccgtc gcggtccagg
     3541 atgacgagct tcattgcgct agcttggaga tgtcggcgac ccgattcatc gcatcgcgca
     3601 ggtcgttgag cagcgcgaga cggccttggc gaagctcggc atcatcgacc atcaccatga
     3661 cggagtcgaa gaaggcgtcc accggcttct tcaggacggc gaagctcttg agatagccgg
     3721 tgtagtcgcc gcgctcgaac ataggcagcg cattgcctcg ggtcatctcg agcgcggtga
     3781 agagcgcgag ctcggccggc tccttcagtg ccttgcgctc caccggcttg aacgattctc
     3841 cttgggccgc cgcctgtcgc aggatgttag cgacgcgctt gttcgctgcg gcgaggcttt
     3901 cggcttcggg cagctcctgg aaagcccgta ccgcctcaag ctgctgcggc acgagatcta
     3961 ggcgggccgg attctggctc agcacggcgt ccacctgaag cgtcgaaaat ccttggtcct
     4021 ttaggtagcc ggcgaagcgc tcgaggacga aagcatgcat ctccgcgcgg gcgtccttga
     4081 ccttgctcgc atatgtgttg aacgccgagt cgaccagagc gtgccacgat aagccgagct
     4141 tgcgctcgac caggactcga accacgccga gcgcggcgcg acgcaggccg aaagggtctt
     4201 tctccccagt gggctgctgg ccgatgccga acatgccggc gagcgcgtcg agcttgtcgg
     4261 caagcgcgac cgcgcaggcc acttcgtcgt cgggaagccg atcgccggcg aagcgcgggc
     4321 ggtagtgccc ctggatcgca tcggcgaccg ggcgcggctc gccgtcattg agcgcgtagt
     4381 agcggcccat cacgccctgc agctccggga attcgccaac catgccggtg agaagatcgg
     4441 ccttcgacag ccatgcggcg cgctccgccg cggcaacgtc ggcactcagg cttctcgcga
     4501 tatggcccga aagcatctgg acgcgcatga cgcggtcgag ctggctgccg agtttgttgt
     4561 ggaagatcac tttggcgagc tccggcacgc gcgcttccag cctgaccttg cggtcctgct
     4621 cgtagaagaa gcgtgcgtcc tcgaagcgcg ggcgcacgac gcgctcgttg ccggcgatga
     4681 tgtgctgcgg gtcggcgagc ttcatgttgg atacgatgag gaacctgggc acaagcttgc
     4741 cggcgccatc gaagagcggg aagtacttct ggttctgccg catcgtgagg atcaggcatt
     4801 cctgcggtac ggcgaggaac gcggcgtcga agctgccgac atagacgctc ggatgctcga
     4861 cgagcgcggt cacttcgtcg agcagatctt cgtaggcacc tagcgctccc ctttcgcgcg
     4921 ccgcctcggc cttgagctgg cgctcgatct catcgcgtcg ggcggcgaaa tccgcgatca
     4981 ccatgccttc ctcgcgcagc gtcgtctcgt acttcgtggc atccgcgagt gtaatcgtgc
     5041 ctttgcccat gaagcggtgg ccctgggtgc gattggtgga ctccaggtcc agcacgcgtc
     5101 cgggcacgac ctgccggccg tgcatcatga cgaggccgtg caccggtcgg acgaactgcg
     5161 catcgccgct cccccagcgc atgagcttcg cgaccggaag ccgcttcagc gcctcttcga
     5221 cccggagcgc aagattcgcg gcgagatgag accctgccgc aattctccgg tatgcgaaaa
     5281 cctctccttt cggcgtatca agctttgtca agtcgtcgac agacaccccg tttttctttg
     5341 cgaaccccgc gaccgcttgc ggcggcgcgt ttaccgaggg gcccgtcagc tgaagctgtc
     5401 gatccggaga tttctcatgc acatccgtga tcgaaaccgc aagccgacgc ggagtcgcga
     5461 acgccgtggc tgcgctgtcg ccgctgagaa agccctctcc tttcagcgcc gcgaccagcg
     5521 cgctcgaaaa tgcattcgcc aacgcgcgca gcgacttggg cggcaactcc tcggtcagca
     5581 gctcgacgag cagcgtgagc gccatctatg tcgccttcga cagcatggga aagccgaggc
     5641 gttcgcgcga ctcgtaatac ccatgcgcga cttccttcgc gagcgtacgc acgcgaccga
     5701 tataggcctg gcgctcggta accgagatgg cgccgcgcgc atcgagcagg ttgaaggtgt
     5761 gggaagcctt catcaccatc tcataggccg gcaggacgca tcgcgcggcg atcaggcgtt
     5821 ttgcctcagc ctcgaagtcg ccgaaatgcc ggaacagcat atcggcattg gagagctcga
     5881 agttgtactt tgactgctcc acttcgttct ggtggtacac gtcgcgataa gcgacacccg
     5941 gcgtccagat gaggtcgaac acgctgtcct tgccctgcag gtacatggag aggcgctcca
     6001 ggccgtaggt gatctcaccg agcaccggct tgcaggcgag gccgccgact tcctggaaat
     6061 aagtgaactg cgtgatttcc atgccgtcga gccacacttc ccatccgagg ccccaggcgc
     6121 cgagagtcgg cgattcccag tcgtcctcga caaagcggat gtcgtgctgc gccgggtcga
     6181 ttcccaaggc cttcagcgag ccaaggtact tgtcctggat ttccaccggc gagggcttga
     6241 gcacaacctg gtattggtag taatgctgta gacggttcgg gttatcgcca tagcggccgt
     6301 ccttgggtcg gcgcgacggc tgcacgtacg ccgcgctcca cggctcggga ccgatcgcgc
     6361 gcaggaaggt ggcggtgtgg aacgtgcccg cgccgacctc catgtcgtag ggctgcagca
     6421 gtgcgcatcc ttgccggtcc cagtatgcat tgagctgccc gatgagctgc tggaatgtcg
     6481 gcacgcgcgg attttaacgg ctgcgcttcg ccctcacggt gaacaacgcg aaaatcagaa
     6541 ggcaggcgac gaccaccggc cagtcgcgca ggcgaacgta cggcgtggct cccgcatatc
     6601 cttgcgccgg gatctcgagc cggccctcgg tgaactgctc caggcgagcg agcacgcgcc
     6661 cgtcgcggtc gatcgccgcg gtgattcccg tattcgtcgc agtgagatgc atacggccgg
     6721 tctcgatggc gcgcaagcgg gcgatttgca ggtgctgcgc cggagcgagg gaatcgccga
     6781 accacgcgac gttcgacata ttcacgagca gggtcgcctc gggcaggcta cgcgcgatct
     6841 cagcgccgaa aacgttctcg tagcagatgt tgaccgcgac gcgttggccc gcaacctcga
     6901 gcggcggctg atcgaaggag ccgcggctga agtcggagag tgggatggaa agcacgtcga
     6961 gcgtccagcg gaagcccggg gggacgaact cgccgaatgg tacgagatgc accttgtggt
     7021 agatctgcct tggcgatacg ccgaggctga gcgcgctatt gtagtaagcg tcgcgccccg
     7081 ttcggtaggg caccccaatt agtaggtcgc cctcattacg cttgccgacg gcatcaaggc
     7141 gcgcgagata ggcagggtcg atgcggtcga ggaacgccgg cagcgcggtc tcgggaagga
     7201 tcacgagccg cgcctgggtg ccttcggcga gccgcgcgta ggtctctaga atgcggggtg
     7261 ctcgctcggg atggaacttc atctcctgtt cgatgtttcc ctgcaggagc gcggttggga
     7321 ccggcgcccc ggccggcacc gtccacgcga cgtggcgcag cgcctcgccg gtgccgacca
     7381 cgagcaggag cacgacaagg aatcgaggtt tctgtcccgc gaggagccag agcattccgg
     7441 cggcagaaac aatgacgaac gaaaccatgt acacgccgcc cagcggcgcg tatccctgca
     7501 ggggccatcc cgccgccgaa tagccgagcg cgagccaggg aaagccggtg aagatccacg
     7561 aaagcatcca ttcgcacagc acccaggtcg cggggataag caggacggcg cgcgcagcgt
     7621 cggatgcggg cacgcgcgct tgaagccagc ccgccaccgc gggaaagagc gcaagcaacg
     7681 cgcagaacac aagcgtcgca aaaacggcga gcggcgccgc cataccgccg aactgatgca
     7741 ggctcacata cacccacgac acgcccgcgc cgaagagccc cgccccgaag gcaaacccaa
     7801 gcaaagcgca gcgctgcggg ggcgcgcgca tccacagatg gataaggagc gccaacgtga
     7861 gcagcgcaaa cgggtgcagc ccaaacggcg cgaatgcgag gacggtcgcg gcgcccgcga
     7921 ggacggaata cagcaaatgc gagcccggga tcagctcttg agtctgtcga ccagtagggt
     7981 gtagacgcgg cggctgtcgg cgcgcagcac ctggaagcgc aggccctcca tcactacgga
     8041 ctcaccgcgc ttcggcaggc ggccgaggtg cttgatgaga tagccgccga tggtgtcggc
     8101 cgcctcgtcg gagagatgcg tgccgaaggc ggcgttaaag tcggcgatct gcgtgagcgc
     8161 cttgacccgg tgccggccgc ctggctcggg caggatgttg tcggaggcct cgtcgaagtc
     8221 gtattcgtcc tcgatgtcgc ccacgatctg ctcgagcacg tcctcgatcg tcaccaggcc
     8281 cgcgacgccg ccgtactcgt cgatcacgat cgccatgtgg ttgcggctgg cgcggaactc
     8341 gcgcagcagc acgttgagcc gcttcgcctc cggaacgaac accgccgggc gcagcatctc
     8401 gcgcacgttg aactcctctt cgccggcata aaaacgaaga aggtctttgg ccagcaatac
     8461 gcctatgacg tcgtcgcggt tctgatcgat caccgggaag cgcgagtgcg cggtggcgat
     8521 cacctgcttc acgaacttct ccaccggctc gttgatgtcg atgaagtcga cctgggcgcg
     8581 cggcaccatg atgtcgcgca ccgccatctc cgacacggtg agcgctcctt cgatgatcga
     8641 gagcgcgtcc gcgtcgagca ggttgcgcga ataggctgac cgcagaagct gcgtgagctc
     8701 gctgcggtcg ccgggctcgc gcatcagaag cgccgaaagg cgctcgagca ggcttggtcg
     8761 ggactgatcg ttcatgcctg gcgcgttcat tctactccgt aggggttggc gattccggcg
     8821 ctgcgcagaa tgcgaatctc gcgcgcttcc atgcgcgccg catccctttt cttttcatgc
     8881 gcgtagccgt gcaggtgcag gcatccgtgc accacgaggt gtgcatagtg cgccgcaacg
     8941 gtcttcccct gcgcgcgcgc ctcgcgccgg atgacagggt ggcacagcgc gatgtcgccg
     9001 gtgttgaatg tcagcacgtt cgtcgcgcgg tcttttcccc tgaaggcctt gttgagcgcg
     9061 cggccctcgc gctcgccgac gaccctcaag gtcaggggag accgcgacgc ggcaagcgcc
     9121 cacttgcgca gagatgcagc actcggcact ccgcgccgcg gcactgcgta ttgaacaaag
     9181 atcctactct tcctgctgcc gcgagtcgta ggcatcgatg atgcgcgcga ccagcgggtg
     9241 gcgcaccacg tcctgcgcgc cgaactgcgt gaaggcgatg ccgcgcacgc cggtgaggat
     9301 gcggcgcgcc tcgatcagcc cgctcttctg accgcgcgcc aggtctatct gggtgacgtc
     9361 gccgttgacg accgctttgg cgccgaagcc gatgcgcgtg agaaacattt tcatctgctc
     9421 cggcgtcgtg ttctgcgctt cgtcgaggat gatgaaggcg tgattgagcg tcctgccgcg
     9481 catgtaggcg agcggcgcga gctcgatcgt gccgcgttcg agcagcttgc cggtcttctc
     9541 gaaacccatc aggtcgaaga gtgcgtcgta cagaggccgc aggtagggat cgaccttctg
     9601 cgcgaggtcg ccggggagga atccgagacg ctcgcccgcc tcgaccgcgg gccgcacgag
     9661 cacgatgcgc ttgaccttgt cgcgctccag cgcatcgact gcgcatgcta ccgcgaggta
     9721 ggtcttgccg gtgccggccg ggccgatgcc gaaagtaaga tcgtgcgcga ggatgctttc
     9781 gatgtactcc acctggtgcg gcgtgcggcc gtgcaggtcg gcgcgccggg tgagcagctg
     9841 cggcccttcg ttctccagtg agattttctt ggacagctcg gtgaggccga gctggacgtc
     9901 ctcgactgtc agatcgcccg aggcgcggtc gtagaaatgc tcgatggctt ccgccgcgcg
     9961 ctgtgcctgc tcgcccttca cggtgaactt cgcgccgcgg cgagcgatcg tcacgtcgag
    10021 cgcggactcg atctggcgga tattttcgtc gagcgcgccg cacaggcgtg cgaggcgctg
    10081 gttgtcgacc gggtccagct tgacttcgac cggtttcgcc ctcagacgag ctcaccccgc
    10141 aggctgtgag ggagggcggc ggctatgcgc aggtgcacgt actgcttaat gagcgattcc
    10201 ggcccgggaa agttcaccac acggttattg cctgtgcgcc cctgcagttc ggtcttccgt
    10261 ttcttggaat ggccctccac cagcacgcgc tgggtcgttc cgaccatcgc cgagctgatg
    10321 gcgcgcgcct gtgcttcgag ctgcgcttgc aggcgcagca gccgttcgat tttttcggaa
    10381 tgagaaaccg caccgggcag ctccgccgct ggggttcccg gccgcgggct gtagacgaac
    10441 gagaacgaca cgtcgaaatc ggcgtcgcgc gcgagcttga gcgtcgcctg gaagtcggcc
    10501 tcggtctccc ccgggaagcc gacgatgaag tccgatgaca ggctgatacc gggccgcgcc
    10561 tggcgcaggc ggcgcacgat cgagcggtac tcgagcgcgg tatagccgcg cttcatcgcc
    10621 gcaagcacgc ggtccgagcc ggactgcacg ggaagatgga catgtggcgc gagcttgtcg
    10681 atgtcgcggt gcgcgtcgat cagccgctgg gtaaattcgc gaggatgcga ggtggtgtag
    10741 cggatgcgca ccagctccgg tatttcgtcc aggtagtaga gcagctcggc gaaatccgcc
    10801 ggcgcctcgg cgatgtcccc gcgccaggcg ttcacgttct gtccgagcag cgtcacttcc
    10861 ttgacgccct gcgccgcgag gccggcgagc tcggcgatca cgtcgtcgaa cggacgcgaa
    10921 acctcttcac cgcgcgtgta gggaacgacg cagaagctgc agtacttgct gcacccctcc
    10981 atgatcgata cgaacgcaga ggcgccttcg actttcgcgg gcggaaggtg atcgaacttc
    11041 tcgatctccg ggaagctcac gtcgacctgc ggctggccgc tcgcgcgccg gcgctcgagg
    11101 agctcaggca ggcggtgcag cgtctgcggg ccgaacacca cgtcgacgta gggggcgcgt
    11161 tgcacgatcg ccggcccttc ctggctcgcg acacagccgc ccacggcgat catgacgctg
    11221 ggacgctgcc gcttgaggtg cttgacccgg ccgaggtcgt tgaagacctt ctcctgcgct
    11281 ttctcccgca ccgagcaggt attgaagatg atgacatcgg cctgcgcagg gtcgggcgtg
    11341 agctcgagag cctcggcctc gcgcatcacg tccgcgatcc ggtccgagtc gtactcgttc
    11401 atctggcagc cgaacgtgcg gaggtagagc ttcttcactt tgggaagggc gcgggcggca
    11461 gcgccgcctt ttcggcgtcc gacagaatcc agacccggtg caccatgccg gtcgagtcca
    11521 cgaggtagcg gacctgcgtc ttctccacga gtgatacggg cagcaccagc cggttgtccg
    11581 ggtctcggat ctgcacgccc ggcgcaaggc tgcgcggctt gccgtcgatc tcgaccacca
    11641 tggcttcgag gtggcgcagc tggccgagga ccgcctcctt agggatggtt cgcagctgag
    11701 ccatggccag agcggcgcag cttgcgagaa tggcgaaaac gaggacgcgc atcaggtgtc
    11761 catgatctca aaatcgcgcg tcacttcggc ggtcttcagg agcatggcgg tggccgagca
    11821 atatttctca tgcgacagac ggatcgcctg ctcgacgagg ttgcgcttga gggtgcggcc
    11881 gcgcaccacg aagtgcatgt gtaccttggt gaacaccttc gggtcggtcg cggcgcgctc
    11941 agccgagagc ttcacctcac atccggtcac gtcttgcccg ctttttttca gaatcacgac
    12001 gacgtcgtag gcagtgcagc cgccgctgcc ggcaagaacc agctccatag gccgtggcgc
    12061 aagattgcgc ccgcccccct ccggtgcgcc gtccatggca accaggtgct tacttccggt
    12121 ttcggcaatg aagctcatgc cgtctgcggt ccagcgcacg gtgcaatcca tgacactaat
    12181 ctagcttttt tgtgtaaaat ccgcagcttt tccgctctcg gcatgactac cttcagcgca
    12241 aaaacaggcg aggtccagca cggctggtac gtggtcgacg cgcaggacaa agtccttggg
    12301 cgcctcgcct cacaaatcgc agcgcgcctg cgcgggaagc acaagccgca attcacacct
    12361 cacgtcgaca ccggtgacta catcgttgtg gtgaacgccg cgaagctgcg tgtcaccggc
    12421 cgcaagcccg agcgcaagat ctattaccgt cacagcgggc ggcccggcga gctgcatcag
    12481 atcaatttcg ccaagctgca cgccaagcgc cctgagcgcg tgctgcagat tgcggtcaag
    12541 ggaatgctgc cgaaagggcc gctcggctac gcgatgatga agaagctgaa agtttacggc
    12601 ggcgcgaccc atccccactc ggcgcaacag ccgaaggcac tggagatcta gcatggtcgg
    12661 cgactattac tacggcacag ggcggcgcaa gagcgcagtg gcgcgcgtgt ttctgaagcc
    12721 gggcaagggc gagttcaagg tgaacggcaa gcccgtcgac cagttttttg gccgcgagac
    12781 cgggcgcatg gtggtgcgcc agccgctcga gcttaccaag agcctgtcgt cgtttgacat
    12841 catggtgaac gtgtacggcg gcggcgaaag cgggcaggcg ggagcggtgc gccacggcat
    12901 cgccagagcg ctgatcgagt atgatgcggg actgaagagc gcgctgagca cggccgggct
    12961 ggtgacgcgc gacgcgcgag aagtcgagcg aaaaaaggtc ggcctgcaca aggcaaggcg
    13021 gcgcaagcag tactcgaaac gctgaataag cggattccgc gaaccgaaag ccgccaattg
    13081 gcggcttttg ctttttatga ttagagcagg aattgtcgga ggaacgggat atacgggcgt
    13141 cgagctcctg cgcctgctcg ctcagcatcc cgacgttgag ctgaaggcga tcacctcgcg
    13201 caaggaggcc ggcacaccgg ttgcgcgcat gtttccgagc ttgcggcggc gcgtcgagct
    13261 cgctttcagc gagccgagcg cgcgtgcgct cgccggctgc gacgtggtgt tttttgccac
    13321 gcccaactgt gtcgccatga gcgaggcacg cgcggtgctc gagggaggcg cccgcatcgt
    13381 ggatctctcc gccgatttcc gcattcacga cgtggcgcag tgggaacgct ggtacaagac
    13441 caagcacgcc gcgcctgagc tcatccgcga agcggtctat ggactcccgg aaatgaaccg
    13501 agaacagctg cggcgtgcgc ggctcgtggc gaacccgggc tgctatccaa cgtcaatcca
    13561 gctcggcttc ctgccgctcg tcgaggctgg cgtcgtcgac gtggagcacc tgatcgcgga
    13621 cgcgaagtcg ggcgcgagcg gagccgggcg caagaccgag cttaatctca tgttctccga
    13681 agcctccgac aacttcgccg cttaccacat cacggggcac cggcactggc cggagatccg
    13741 gcaaggcctt gcacaggcgg cgaagcgcga tgtcgggctc gtgttcacgc cgcatctcac
    13801 gcccctgatc cgtggcattc acgcgactct gtacgcccgc attacccggg aggcggattt
    13861 ccagcagctt ttcgaaaagc gctacgcggg agagccgttc gtcgacgtca tgcccgcggg
    13921 cagccacccc gatacgcgtt cggtgcgcgc cgccaatgtg tgccgcctcg cggtgcatcg
    13981 accgccggag agcgccggcc gctccgacac catcgtagtg ctgtcagtta tcgacaacct
    14041 gatgaagggg gcctccggac aggcggtaca gaacatgaac ctcatgttca gcctgccgga
    14101 aacagccggg ctcgaacacg tggccgtggt gccttagatg ccgtcgggtc gctggaaagt
    14161 cattcgcagc cgcttcggca tcgcggcccc gcgcgtcgag attcataccc aaatcccgtg
    14221 gtacttgcgc tgggttggca ttgccgtcct cctcggcctt gctgccatag cggcgtcctg
    14281 gatctacgat gccggcgggc gctttgccgg ctttgatcgc agcgaggtgc tgcaggagct
    14341 gcagcgagcg caaggcgagc tcgacaacgc gcgcgaggag cttgagcgcc ttcgagccat
    14401 ggcgaatgcg gccgacagcc gcgtctccat cgagcgcacg gcgcagcaga agctcgccca
    14461 gcaggtgcgc gcgctggagc aagaaaacgc tcgcgtgcga gaagagcttg cgatggtcga
    14521 gcagatgctc acatcggaag aacgtcacac tcagaccctg gccatctacc agttcaaggt
    14581 ccagccggac gttctgccgg gcgaataccg ctaccggctg cttctgctaa cgccgaccga
    14641 ccggcgcggc cgcgacttca gcgggcggct cgagctcgtc gtcaacctgc acgaaggagg
    14701 gcagaatgct atgatgtcct tcccggagcc gggcgacgcc gcggcagcgg cattccggtt
    14761 agcgttcaag tatttccgtc gggtcgaagg cactttccgg gtgagcccga aggcgaaggt
    14821 ggagagcgtg caagtgcgga tctacgagac tgggtcgagc cagccgcgcg cgacccagtc
    14881 ggtgacgctg tagcgctcaa gggaagaagg agcggatacg tgttcaacaa gacgagcaag
    14941 ccgcagaaca ggatcgacag cctgatcggt gcaaccaccc gcatcgaagg caacgtgatc
    15001 ttcagtggtg gattgcgtgt ggacggcatg gtgcgcggca acatcgccgc acttcccgac
    15061 cagccgggca cgctcgtcgt ttcgtccgac gggcgcatcg atggcgaagt gcaggccgcg
    15121 cacatcgtgg tgagtggcac gatccagggg ccggtgcatg ccaccgagac gctcgaacta
    15181 caggcaggct cgcgggtcaa aggagacgtg tactacaaga gcatagaggt ccatcagggc
    15241 gccgtggtcg agggccgcct ggtgcctcac cccgccgagg cgaaaggggt cgagctcaag
    15301 ctcgcctcgg gcggataaat cgcaaaggaa cgctgaatga acgcagttac cgagatgccg
    15361 gcgccgcttg tctttactga caacgccgcc aacaaggtca agcagctgat cgaagaggag
    15421 ggcaacccgg agctcaagct ccgcgtgttc gtcaccggcg gcggctgctc gggtttccag
    15481 tacggtttca cgttcgacga ggtgcagaac gacgaggacg cctcgatgga gaagaacggc
    15541 gtcacgctgc tcatcgaccc gatgagctac cagtacctgg tcggcgccga gatcgattat
    15601 caggaaagtc tggaaggcgc gcagttcgtg atcaagaacc cgaacgccca gagtacctgc
    15661 ggctgcgggt cgtctttctc cacctaagca acaggtttcc cgcgcaaagg gcccgttcgg
    15721 gctctttgtc ttttctagtc cagccgggcg agcgcgctgt tcgcgatcag atcgagccgt
    15781 gtgatgagcg gctcggcgtg gctgcggaat gcagcaaggt cctgcggcgt caccggcagc
    15841 gcggctggca tcgcgacgga caacgggttg cgcgcctcgc cggcaacgcg gaactcatag
    15901 tgcaggtgcg gtccggtcgc ccagccggtc tggccgaccg cgcctatggt gtcgttctgc
    15961 gcgacgcgct ggccgcggtg cacgttgatg cggctcaggt gagcgtagat cgtcgagtac
    16021 tggccatgat ggcggaggat cacgaccttg ccgtagccgc ctttcaggcc ggcgtgctcg
    16081 acgacgccgt caccgactgc acgcacgcgc gtgccgctgg gcgcggcgta gtcgatcccc
    16141 tggtgcttga tccaagcctg tgcgatcggg tgacggcgca tgccgaaacc cgagctcacg
    16201 cgggaaaact ccagcggcga gcgcagaaac gccttgcgga ggttcttgcc ttcgggcgtg
    16261 aagtagccag tgcggaagtg caccgcacgg aaggtcttgc ggttattggt gaactcggcg
    16321 gcgaggacgc ggcccgcgcg tacggggcgc ccgcccaggt agtgcagttc gtagaccacg
    16381 gcaaactgat cacccttgcg cagatcacgg tgaaagtcga tgtcgccgcc gaacacctcg
    16441 ccaagctgca tcgcgatgct gtccgggata ccggcgtcat ccgcggcggc aaaaagtgaa
    16501 ctctcgatga cgcccgattt catcgcgacg cgagtgtaga gctgcgcccg gtcgtcggta
    16561 acgcggaact ggccgttcgc ttcggccgcc accttcacca cggtgtcgcg tccggacaga
    16621 aaagacagca cgagcggctc gccctcggcg ctaacttcgg cgctgacatg cgttcccggc
    16681 cgcagctgct gcagcacgcg gagcttcgtc aggcgcgcag cgtgtggtgc ctcgataccg
    16741 aggcgctcga ggaacgcggg cagggtatcg ccgcgttgaa agagctcttc gcggaaatac
    16801 gacgtcggcg caggcgtggc gtcggcccgg atctcgagcg cctccacgat cggccgccgc
    16861 ggcggctgaa ctcgttcttc ggcgcgcggc gcaatggtcg cgaaggccac tacgccggaa
    16921 gcggcgagga gtccgatcgc ggcgatgccg cccagccgtc cgagagtcct catggatccg
    16981 ctagaatccc ttgaatttca taagcctgca aagtctagca aagaacccaa ggtagagaaa
    17041 atcaccatgt cggccatcag gcaggccatt cagaccattc gtcggggcgc cgaggagttg
    17101 atcgtcgagg aggagctgaa aaacaagctc gcgagcggcc gcaagctgcg catcaagctc
    17161 ggcctcgacc cgaccgcgcc cgatctgcac ctcggacata ccgtggtaat caacaaggtg
    17221 cgcgacttcc agaacctcgg ccaccaggtg cagtttctga tcggggattt caccgggatg
    17281 atcggcgat
//
LOCUS       DASTPD010000135         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_70398, whole genome shotgun sequence.
ACCESSION   DASTPD010000135 DASTPD010000000
VERSION     DASTPD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70398"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFR57_04775"
     CDS             complement(<1..559)
                     /locus_tag="VFR57_04775"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEU0258721.1"
                     /translation="MPPIVLTFAASDPTGGAGLQADVLTIAALGCHPLSVLTGLTVQD
                     TRGVDHLEPIAEELVTRQANCVLAESKVAAFKVGVLASADNVRAVAQVAAAHAHVPLV
                     LDPVLASGRGDELASNAVQAALLEWLVPRATVVTPNTVEAQRLGGVKHLLERGCRYVL
                     VTGTHEPSSEVTNKLYGPAGLVREDR"
     gene            583..771
                     /locus_tag="VFR57_04780"
     CDS             583..771
                     /locus_tag="VFR57_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEU0258722.1"
                     /translation="MSTESQVQFRTWMCLICGFVYDEATGLPDEGIAPGTRWEDVPMN
                     WTCPECGARKEDFEMVEL"
     gene            835..1395
                     /locus_tag="VFR57_04785"
     CDS             835..1395
                     /locus_tag="VFR57_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0258723.1"
                     /translation="MSSINLTHHFLIAMPNMADPYFSKTLTYICEHNDQGALGLVVNR
                     PIDMTLQALFERLSLTLRDANRKDAPIYFGGPVQTDRGFVLHEPAGNWQSTLRVRDVI
                     GLTTSKDILEAVGRGEGPARLLVTLGYAGWSPGQLEHELSQNAWLTVEARDGIIFDTP
                     AEERLPAAMELLGLDYARLQDAAGHA"
     gene            1388..1774
                     /gene="ruvX"
                     /locus_tag="VFR57_04790"
     CDS             1388..1774
                     /gene="ruvX"
                     /locus_tag="VFR57_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0258724.1"
                     /translation="MPERGTVLAFDFGLKRIGVAVGEPEIGTAHPLPAVSSFSQIQRL
                     VEEWKPSSLVVGRPTSTKGEPQTMTRQAEDFARRLERRFKLPVARVDERYTSVEAEHR
                     LRGVKRKAIDSVAAQLILEQYFDEAA"
     gene            1775..2278
                     /gene="pyrR"
                     /locus_tag="VFR57_04795"
     CDS             1775..2278
                     /gene="pyrR"
                     /locus_tag="VFR57_04795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU0258725.1"
                     /translation="MKLPDAEKLCEQLAAELKPRIGPKTAMVGLYTGGAWFAERLHLM
                     LQMTRPLGLMDIAFYRDDYAARGLKHDPKRTKIPFDVNGCELLLVDDVLYSGRTVRAA
                     MNELFDYGRPASIALVVLADRGGRQLPICAQHVGARVEVPAGMRLTLKREESGKLALA
                     LEPQRAA"
     gene            2268..>2917
                     /gene="pyrB"
                     /locus_tag="VFR57_04800"
     CDS             2268..>2917
                     /gene="pyrB"
                     /locus_tag="VFR57_04800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600263.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0258726.1"
                     /translation="MQRNPQLNEKGELQHLLSIEGLPREIVLQILDTAESFVGVTERE
                     VKKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVINLNITVSSATKGESLLDTV
                     ANLSAMQADIFIVRHESSGAPFLIARHVKAHEHVINAGDGRHAHPTQGLLDLFTIRHY
                     KKDFTRLTVAIVGDILHSRVARSLIHGLTTLGVPEVRVIGPQTLLPAGVEALGVRV"
BASE COUNT          531 a          933 c          949 g          504 t
ORIGIN      
        1 agcggtcctc gcgtacgagg cccgccgggc cgtagagctt gttggttact tccgagctcg
       61 gttcgtgcgt tcccgtaacg agcacgtaac ggcagccgcg ctcgaggaga tgcttcacac
      121 cgccaaggcg ctgcgcctcc acagtgttcg gcgtgaccac ggtggcgcgc gggacaagcc
      181 attccagcaa agcggcttgg acagcgttgc tcgccaattc gtcgccgcgg ccagaggcga
      241 gcaccgggtc gagtacgagc ggcacatgcg catgcgcggc cgccacctga gccacggcac
      301 gcacgttgtc tgcggacgcg agcacgccca ccttgaacgc tgcaactttg ctttcggcga
      361 gcacgcagtt cgcctggcgc gtcacgagct cttccgcaat cggttccagg tggtcgacac
      421 cgcgtgtgtc ctggacagtg agtcccgtga gaactgacag cggatggcag cccagggcgg
      481 cgatggtgag cacatcggct tgcaagccgg cgccgccagt gggatcggat gccgcgaacg
      541 tcagcactat cggtggcatg ggttaatatc cgcctcgatt ttatgagcac cgagagccaa
      601 gtacagttca gaacgtggat gtgcctgatc tgcggcttcg tgtacgacga ggccacgggc
      661 ctgccagatg aaggcatcgc accgggcacg cgctgggaag atgtgccgat gaactggacc
      721 tgccccgagt gcggcgcccg caaggaagat ttcgagatgg tcgagctctg aggcgcaacc
      781 tttcggcgcg ggcggcgctc taagcccttt gacagtagaa tgagccttgg gcggatgtcc
      841 tcgatcaacc tgacgcatca cttcctgatc gccatgccca acatggccga tccgtacttt
      901 tccaagacgc tcacctacat ctgcgagcac aacgaccagg gcgcgctcgg cctggtcgtg
      961 aaccggccga tcgacatgac gcttcaggcg ctgttcgagc gcctttcgct caccctgcgc
     1021 gacgccaacc gcaaggacgc gccgatctac tttggcgggc cagtgcagac tgaccgcggc
     1081 ttcgtgctgc atgagccggc gggcaactgg cagtcgacgc tgcgcgtgcg cgacgtgatc
     1141 gggctcacca cgtccaagga cattctcgaa gcggtggggc gcggcgaggg gccggcgcgc
     1201 ctgctggtga cgctcggcta cgccggctgg tcgccgggtc agcttgagca cgagctgtcg
     1261 cagaacgcct ggctcaccgt ggaagcgcgc gatgggatta ttttcgacac cccggcggaa
     1321 gagcgcctgc cggcagcgat ggaactcctc ggtctcgact acgcgagact gcaggacgcg
     1381 gcgggacatg cctgagcgag gcaccgtcct cgctttcgac ttcgggctta agcgtattgg
     1441 ggtggcggtg ggggagccgg aaatcggcac ggcccatcca ctgcccgcgg tttcgagttt
     1501 ctcccagatc cagaggttgg tggaggaatg gaagccgagc agcctggttg tgggtcgacc
     1561 gacttcgacc aagggcgagc cgcaaacgat gacccgccag gcggaggatt tcgcccgacg
     1621 cctggaacgg cgcttcaagc tgccggtggc gcgcgtggac gagcgctaca cgtcggtaga
     1681 ggccgaacac cggctgcgtg gcgtgaaaag gaaggccatc gattcggtcg cggcgcagct
     1741 catactcgag cagtactttg atgaagctgc atagatgaaa ttacctgacg cagaaaaact
     1801 ctgcgagcag ctagcggctg aactcaaacc ccggatcggg cccaagaccg cgatggttgg
     1861 cctttatacc ggcggtgcct ggttcgcaga gcggctgcac ctgatgttgc agatgacgag
     1921 gccgcttggc ttgatggata tcgcgttcta ccgcgacgac tacgcggcgc gcggcctgaa
     1981 gcatgatccc aagcgcacca agatcccgtt cgacgtgaac ggctgcgaac tgctgctggt
     2041 ggatgacgtg ctctacagcg ggcgcacggt gcgcgccgcg atgaacgagc tgttcgacta
     2101 cggcaggccc gcaagcatcg cgctcgtcgt gctcgccgac cgcggcggcc gtcagttgcc
     2161 catttgcgcg cagcacgtcg gcgcgcgggt cgaagtaccg gccggcatgc gcctcaccct
     2221 gaagcgcgag gagagcggaa agctcgcgct cgccctggaa ccccagcgtg cagcgtaatc
     2281 cgcagctgaa cgaaaagggc gagctgcagc atctcctttc catcgagggg ctgccgcgcg
     2341 agatcgtgct gcagatcctc gataccgccg agtccttcgt cggcgtcacc gagcgcgaag
     2401 tgaagaaggt accgctcctg cgcggcaaaa gcgtgttcaa cctgttcttc gagccttcga
     2461 cgcgaacacg caccactttc gagatcgccg cgaagcgtct ttcggccgac gtcatcaacc
     2521 tgaacatcac cgtctcgtcg gcgaccaagg gcgaatcgct gctcgacacc gtggccaacc
     2581 tctcggcgat gcaggccgac atcttcatcg tgcgccacga gtcctcgggc gcgccgttcc
     2641 tgatcgcacg tcacgtcaag gctcatgagc acgtgatcaa cgcgggggac ggccgccacg
     2701 cgcatccgac ccagggattg ctcgatctct tcaccatccg ccactacaag aaggacttca
     2761 cgcggctcac ggtggcgatc gtcggcgaca tcctacattc ccgtgttgcc cgctcgctca
     2821 tccacgggct taccacgctc ggggttcccg aggtgcgcgt cattggcccg cagacgctgc
     2881 tcccggccgg cgtagaggcg ctcggcgtgc gggtatt
//
LOCUS       DASTPD010000136         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_479609, whole genome shotgun sequence.
ACCESSION   DASTPD010000136 DASTPD010000000
VERSION     DASTPD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479609"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..860)
                     /locus_tag="VFR57_04805"
     CDS             complement(225..860)
                     /locus_tag="VFR57_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU0258727.1"
                     /translation="MRRLPCDRHDGKQGRGLDLRLPEMISPEEQARANLYGLLARLFY
                     APPDAALLKALAGETLEGELARPWRDVSSTAATADPEAVREEYETAFIGTGKAPVTLY
                     TTGYSIRYSNETPLANLRGELARLGLGRRADVYEPEDHIAALCDTMRHLVAEQKAGLE
                     EQRRFFNKWIHPTAGPLCAAIEKNDKTSFYKAVARFAASFFDMEQSAFEML"
     gene            complement(788..2821)
                     /locus_tag="VFR57_04810"
     CDS             complement(788..2821)
                     /locus_tag="VFR57_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0258728.1"
                     /translation="MSLEGKTLKVCSCNRTVPLDAKALAAALKAGEPLSVHHQLCRSD
                     AGAFQAAVKAADDGVIVACTQEAALFSELSEKPIKFVNVREFAGWSAERSTPKIAALL
                     AMAALPEPEPAPAVDFKSQGQVLVIGPAEAALDWAERLSGQLEVSVLLTAAGGELPLE
                     RKYPIWSGKPLRVSGWLGAFEVEWRQENPIDLEVCTRCNACVRACPEQAIGYDYQVDL
                     AKCKSHRKCVAACGDIGAVDFSRSDVLRKESFDLVFDLSREPLVSKHEPPQGYFAPGD
                     DPLEQALSAQKLAGLVGEFEKPKFFFYNARICAHSRSGKQGCNACIDVCSTGAIRPDG
                     DHVTVEPHLCAGCGGCATVCPSGAMTYGYPRVPDLGLRLKTALATYRDAGGKDACILF
                     HDPTEGRAALLSHGRKSKGLPARVIPVELFHAASVGMDLMLGAMAYGASQVCILSTEK
                     VSDGYREALKRQMGYAEAIVNALGYTGRHFTLVENPSELWGLEPAATVAKPATFNLSS
                     EKRTTLDFALDHLAHEEEKTIPLPAGAPFGALTVDKQTCTLCKACIGACPESALIDAT
                     ERPALRFIERNCVQCGLCVNTCPENAISLVPRLLIGRQAKEAVTLNEAEPFNCVRCGK
                     PFGTRQMVENMLGRLGGHSMFVGGTRRLQMCGDCRVIDMMENKAEASIFDYRK"
     gene            complement(2796..3209)
                     /locus_tag="VFR57_04815"
     CDS             complement(2796..3209)
                     /locus_tag="VFR57_04815"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEU0258729.1"
                     /translation="MATEEKKEAFLDRWSRLKKEEKELPVNDDKPVPDLPPVDQLTPE
                     SDFTGFMHPKVEDALRRVALKKLFSDPRFNVADPFEAYSGDWTGGEPIPEEMLAALNQ
                     AKRLLFDDNKEADGEKKEPEKTATPNSDEPGRKDA"
     gene            complement(3210..3671)
                     /locus_tag="VFR57_04820"
     CDS             complement(3210..3671)
                     /locus_tag="VFR57_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEU0258730.1"
                     /translation="MEKPSFLIAVVMQRRPAQSRWADCIWEPHGVIADPGGAPRVLRE
                     DGAVAQWLHPGFKLVLHKDEIEGYYLNVSAPHPKVFVLWRMEDDRALPLDVTVSSDEA
                     GRWLDGGHSIDGVAMPVEIFAWVGEYVENNYRPQPKKRIKPRSFQHPKDRL"
     gene            3720..4778
                     /gene="moaA"
                     /locus_tag="VFR57_04825"
     CDS             3720..4778
                     /gene="moaA"
                     /locus_tag="VFR57_04825"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU0258731.1"
                     /translation="MKKVIPIQPGYAPATDFEGKDLRDTRGREMRDLRISVTDRCNFR
                     CTYCMPRGVFDSSYRFLPHEAILTFEEIARLARVFVSLGVQKIRLTGGEPLVRRDVHR
                     LVALLKELDVELTLTTNGSLLAKHAHALKSAGLDRVTVSLDSLDDATFRAMNDADFPL
                     ARVLEGIQVAADAGLGPIKINMVVKRGVNDDGIVAMAERWRGTGHIIRFIEYMDVGST
                     NGWRMDEVVPSAEVVERISERWPLEPIDRNYLGEVAERWRYVDGAGEIGVISSVTRAF
                     CSTCTRMRLSTEGGLYTCLFAQHGHDLKRLIRAGASDQALRNEIAAVWQERADRYSEI
                     RTSHTSALRKVEMSYIGG"
     gene            4771..5607
                     /locus_tag="VFR57_04830"
     CDS             4771..5607
                     /locus_tag="VFR57_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU0258732.1"
                     /translation="MAKGKRPLLTDAARPATFEVEAYNERGEMVPTAIAGEHPLTLYL
                     DKREVVTLMTLGHAPEALVIGYLRNQRLVKSIDKIAAVQVDWETESAAVTTRSKKRVK
                     ISKKKTVTTGCGQGTMYGDLMEDIEKVKLRNDVRLADSDLFVLLEKVRRHETIYKQAG
                     AVHGCALATTAGEILMFIEDVGRHNAVDAIAGKMWLDDIDGSDKLFYTTGRLTSEMVI
                     KCAQMRIPFLVSRSGLTHMGHEIAKKVGITMLGRASGRHYLAFTGKERIVRTAPQPTA
                     LA"
     gene            complement(5573..>5856)
                     /locus_tag="VFR57_04835"
     CDS             complement(5573..>5856)
                     /locus_tag="VFR57_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258733.1"
                     /translation="MLAVFGQIPLNDVIVGKYVADEYRARVLSVRYVVSLGVAAVAVP
                     LIAVLHRTEGGFGNVFLVLAALAAGMLVASLFFPSRRTITQAQSAAAPS"
BASE COUNT         1050 a         1890 c         1865 g         1051 t
ORIGIN      
        1 tcggtggcgt ggtagccgcg cgtcgcgcgc ttgccgttgc cggatgttgc tgagatggtt
       61 ttcttggtcg ccacgaccgc tgcggcggtc gcggcgccgc cggcgcctac ggtgaacagg
      121 aatttacggc gggacaggcg cgggctacgt tcgctcatga cgtatctctc ctccgtcaaa
      181 gactgcgagt cggtgcaact tcgtgtgcac taattcgcac gattctagag catttcgaag
      241 gccgattgct ccatatcaaa gaagcttgcg gcgaagcggg cgacagcttt gtagaaggaa
      301 gtcttatcgt tcttttctat tgcggcgcac aagggtccgg ccgtgggatg aatccactta
      361 ttaaagaagc ggcgttgctc ttcgagaccg gccttttgct ccgcaacaag atgcctcatc
      421 gtgtcgcaga gggccgcgat atggtcttcg ggctcgtaca catccgcgcg tcgacctagg
      481 cccagccggg caagctcgcc gcgcaggttg gcgagcgggg tctcatttga atagcggatc
      541 gaataccccg tcgtgtaaag cgtgaccggg gccttcccgg taccgatgaa cgcagtttcg
      601 tattcttcgc gcaccgcctc cgggtcggcg gtggcagccg tgctcgatac gtcccgccag
      661 gggcgggcca actccccctc aagcgtctct cccgccaagg ctttgaggag cgcggcatcg
      721 ggcggagcgt agaagagccg cgcaagcagc ccgtaaaggt tggcgcgcgc ctgctcctcc
      781 ggggaaatca tttccggtaa tcgaagatcg aggcctcggc cttgttttcc atcatgtcga
      841 tcacacggca gtcgccgcac atctgcagcc tgcgggtgcc tccgacgaac atggagtgtc
      901 cgccgagcct gccgagcatg ttctcgacca tctggcgggt gccgaacggc ttgccgcagc
      961 gcacgcagtt gaagggctcg gcctcgttga gtgttacggc ttctttcgct tgtcgtccga
     1021 tcaggagcct cggaaccagg ctgattgcgt tctcgggaca agtgttcacg cacagcccgc
     1081 actgcacgca gttgcgctcg atgaatcgca gcgccgggcg ttcggtcgcg tcgatcagcg
     1141 ccgactcggg gcatgccccg atgcaggcct tgcatagcgt gcaggtctgc ttgtcgacgg
     1201 tgagagcgcc gaaaggagcc cccgcgggaa gcggaatggt tttctcctcc tcgtgcgcga
     1261 ggtgatcgag cgcgaagtcg agcgtagtgc gtttctcgga cgacaggttg aacgtcgccg
     1321 gcttcgccac cgtcgccgcg ggctcaagtc cccacaactc cgaagggttt tcgacgaggg
     1381 tgaaatgccg acccgtgtat ccgagcgcat tcacgatcgc ctcggcgtag cccatctggc
     1441 gcttcaacgc ttcacggtat ccgtcggata ctttctcagt cgacaggata caaacctgcg
     1501 atgcgccata ggccatcgcc ccgagcatca ggtccatgcc gaccgaggcg gcatggaata
     1561 gctctaccgg gatcacccgc gcgggcaggc ccttgctctt tcttccatgg gaaagcaaag
     1621 cggcgcgacc ctcagtcggg tcgtgaaaca agatgcatgc atccttgccg ccggcgtcgc
     1681 gataggtggc gagcgcggtc ttcagccgca ggccgaggtc gggcaccctc ggatagccgt
     1741 aggtcatggc gccggacggg caaacggtgg cgcagccccc gcatccggcg cagaggtgcg
     1801 gctcaaccgt cacatggtcg ccgtcggggc ggatcgcgcc ggtggagcag acgtcgatgc
     1861 aggcgttgca gccctgctta cctgagcgcg agtgggcgca gatgcgcgcg ttatagaaga
     1921 agaacttcgg cttctcgaac tcgcccacga gccctgcgag cttctgcgcc gagagcgcct
     1981 gctcgagcgg atcgtcgccg ggagcgaagt agccctgcgg cggctcgtgc ttgctcacga
     2041 gcggctcgcg cgacaagtcg aacacgaggt cgaacgattc cttgcgcaga acgtcgctgc
     2101 gcgaaaaatc gaccgcgccg atgtcgccgc atgccgctac gcatttgcgg tggctcttgc
     2161 acttcgcgag atcgacctgg tagtcgtaac cgatcgcctg ctccgggcac gcgcgcacgc
     2221 aggcgttgca gcgcgtgcaa acctcgaggt cgatcgggtt ctcctggcgc cattccactt
     2281 caaacgcgcc gagccagccg ctcacccgga gcggcttgcc cgaccagatg gggtacttgc
     2341 gctcaagagg cagttcacca cccgcagcgg tgagcagtac cgagacttcg agctgccccg
     2401 acaggcgctc ggcccagtcg agcgcggctt cggccggccc gatcacgagc acctgcccct
     2461 gcgatttgaa atctacagcc ggcgcgggct cgggctcagg aagcgccgcc atggcgagca
     2521 gcgcggcgat cttgggcgtg gatctttcgg ccgaccagcc cgcaaactcg cgaacgttga
     2581 cgaactttat gggcttttcg gaaagctcgg agaagagcgc cgcctcctgc gtgcaagcga
     2641 cgatcacgcc gtcatccgcg gccttgaccg ccgcctggaa agcgcccgcg tcgctgcggc
     2701 agagctggtg atgcacgctg agcggctccc ccgccttaag cgctgcagcg agcgccttgg
     2761 cgtcgagcgg caccgtgcgg ttgcagctgc agaccttaag cgtctttcct tccaggctca
     2821 tcgctattgg gggttgctgt cttctcaggt tccttcttct cgccgtcggc ttccttgttg
     2881 tcgtcgaaga gaagacgctt cgcctggttg agcgccgcga gcatctcctc cgggatcggc
     2941 tcgccgccag tccagtcgcc cgagtacgcc tcgaacggat cggcgacgtt gaaacgcgga
     3001 tcgctgaaga gcttcttcag cgccacccgc cgcagcgcat cctcgacctt agggtgcatg
     3061 aagccggtga aatccgactc gggtgtgagc tggtccaccg gaggaagatc cggcaccggc
     3121 ttgtcgtcgt tcacgggcag ttccttctcc tcttttttca gccgcgacca gcgatcgagg
     3181 aaggcctcct ttttctcctc cgttgccatt tacaggcggt ccttggggtg ctggaacgag
     3241 cgcggcttga tgcgcttctt gggctgcgga cggtagttgt tctccacgta ttcgcccacc
     3301 caggcgaaga tctcgaccgg catcgcgacg ccgtcgatgg aatggccgcc gtccagccag
     3361 cgcccggctt cgtcggagct caccgtcacg tccaggggca atgcgcgatc gtcttccatg
     3421 cgccagagta cgaaaacctt ggggtgcggc gccgaaacgt tcaggtagta gccctcgatt
     3481 tcgtccttgt gcaggaccag cttgaagccg gggtgcagcc actgcgcgac cgcgccgtct
     3541 tcccgcagta cgcgtggcgc gccgccggga tccgcaatca ccccgtgcgg ctcccagatg
     3601 caatcggccc agcggctttg cgcgggcctg cgctgcatca ccaccgcgat caagaacgag
     3661 ggtttttcca ccgggcaaac tatacgcgct ctataatgaa tttccgagaa aaacccttga
     3721 tgaagaaggt gattccgatc cagccgggat acgcgccggc gacagatttt gagggcaagg
     3781 acctcaggga cacgcgcggc cgcgagatgc gcgacctgcg tatctcggtc acggaccggt
     3841 gcaacttccg ctgcacctac tgcatgccgc gcggggtgtt cgactcgagc taccgcttcc
     3901 tgccgcacga ggcgatcctt acgttcgagg aaatcgcgcg gctcgcgcgc gtcttcgtaa
     3961 gcctgggcgt gcagaagatt cgactcaccg gcggcgagcc gctggtgcgg cgcgacgtcc
     4021 atcggttggt cgcattgttg aaagaattgg acgtggaatt gaccctgacc acgaacggct
     4081 cgctgctcgc gaagcacgcg catgccttaa agagcgccgg gctcgaccgc gtcaccgtca
     4141 gtctcgactc tctcgacgac gcgaccttcc gggcgatgaa cgacgcggac tttccgctcg
     4201 cccgggtcct cgaaggcatt caagtggccg ccgacgccgg cctcggaccg atcaagatca
     4261 acatggtggt aaagcgcggc gtcaacgacg atggcatcgt ggcgatggcc gagcgctggc
     4321 gcggcacggg gcacatcatt cgcttcatcg agtacatgga cgtcggcagc accaacggct
     4381 ggcgcatgga cgaggtcgtg ccttcggccg aggtcgtcga gcgcatctcc gagcgctggc
     4441 cgctcgagcc tatcgaccgc aattacctcg gcgaggtcgc cgagcgctgg cgctacgtgg
     4501 acggcgcagg cgagatcggc gtcatctcct cggtgacgcg ggcattctgc tccacttgca
     4561 cccgcatgcg gctctcgacc gaaggcgggc tctacacctg cctcttcgcg cagcacgggc
     4621 acgacctcaa acggctcatt cgcgccgggg cttccgacca ggcgctgcgc aacgagatcg
     4681 ctgcagtctg gcaagagcgc gccgaccggt actcggagat caggacctcg cacacctctg
     4741 ccctgaggaa ggtcgagatg tcgtacatcg gtggctaagg gcaagaggcc gctcctcacc
     4801 gacgccgcgc ggccggccac gttcgaggtc gaggcgtaca acgagcgcgg tgagatggtg
     4861 ccgaccgcga tcgccggcga gcaccctctc acgctctatc tcgacaagcg cgaggtcgtt
     4921 acgctcatga ccctcgggca cgcgccggaa gcgctggtga tcggctacct gcgcaaccag
     4981 cgcctcgtga aatcgatcga caagatcgcg gccgtgcagg tcgactggga aaccgagtcc
     5041 gccgcggtta ccacacgctc gaaaaagcgc gtcaagatct cgaagaagaa aaccgtcacc
     5101 accggctgcg ggcagggaac gatgtacggc gatctgatgg aggacatcga gaaagtgaaa
     5161 ctgcgcaatg acgtgcgcct cgccgactcc gatctcttcg ttctgctgga gaaagtgcgc
     5221 cggcacgaga cgatctataa gcaggcgggc gcggtgcacg gctgcgccct tgccaccact
     5281 gcgggcgaga ttctcatgtt catcgaggat gtcgggcggc acaacgcggt ggacgcgatc
     5341 gccggcaaga tgtggctcga cgatatcgac ggctcggaca agctcttcta caccaccggg
     5401 cggctcacct ccgagatggt gatcaagtgc gcgcagatgc gcatcccgtt cctcgtctcg
     5461 cgctcggggc tcacgcacat gggccacgag atcgcgaaga aagtcggcat cactatgctc
     5521 ggccgcgcaa gcggccggca ctacctcgcg ttcaccggga aagagcggat cgtcaggacg
     5581 gcgccgcagc cgactgcgct tgcgtaatcg tccttcttga tgggaagaaa agcgacgcga
     5641 ccagcatccc ggcggcgagc gccgcgagca ccaggaaaac gttcccgaag ccgccctcgg
     5701 tacggtggag caccgcaatc agcggcacgg ccaccgcggc gacgccaagc gagacgacat
     5761 agcgaaccga cagcacgcgc gcgcgatact cgtcggctac atatttgccg acgatcacgt
     5821 cgttcagcgg gatctggccg aacaccgcga gcatca
//
LOCUS       DASTPD010000137         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_889094, whole genome shotgun sequence.
ACCESSION   DASTPD010000137 DASTPD010000000
VERSION     DASTPD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889094"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFR57_04840"
     CDS             complement(<1..514)
                     /locus_tag="VFR57_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0258734.1"
                     /translation="MTAIPNHALRQLRAGKLAIGMGVNQARTVGIATMAKTAGFDWLF
                     IDGEHGSLDGDTVAQIATASLAAGVTPVVRVAGFEHWHASRMLDNGAQGIVFPHVDDS
                     QQASHVANSCRYPPIGKRSMGGALAQLGFASTPVGEAARLVNEETLVVVMIESPQGVA
                     NCEEIAAVKGV"
     gene            complement(511..1890)
                     /locus_tag="VFR57_04845"
     CDS             complement(511..1890)
                     /locus_tag="VFR57_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU0258735.1"
                     /translation="MAAAVYDAIVIGTGQAGPALAARLAGAGRKVAVIERHKFGGTCV
                     NDGCTPTKTMVASAYAAHLASRALDYGVEIAGSLRVDMKRVKARKDAVVSSSSAGVEK
                     WMRGLKGGTIYQGHARFTGPRTLQVGEQTLVSEQIFIDVGGRPLVPKMPGLDTVPYLT
                     NVSMMDVDFVPEHLIVVGGSYIGLEFGQAFRRFGSRVTVVEMAPRLIAREDEDVSAGV
                     KEFLEAEGVEVRLDAECLTAKKTENAVEIGLGCSEGAPVARGSHLLLAVGRVPNTDDL
                     GLDAAGIRTDERGYIEVDEELRTTNPNVWALGDCNGKGAFTHTAYNDYEIVADNLLAN
                     AGRKWTDRIPVYALYTDPPLGRIGLSEKEIKEKGIKALVGKRPMTRVSRAVEKGETRG
                     FLKIHVEESSKRILGAALLGTSADESVHSLIDAVYSKLPYPEFQRRVRIHPNVSELLP
                     FVLEDLKPL"
     gene            2193..>2704
                     /locus_tag="VFR57_04850"
     CDS             2193..>2704
                     /locus_tag="VFR57_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258736.1"
                     /translation="MRKLLPVVLTGAMAVSFSTLAQDADKTRDNVHYNIHQRGDQNTS
                     QYGSGNQSSQVYGSGNQVNQQSGESNAATQTQQQGSDNTSATTTQQGTQNQNAVGTGN
                     QQSQQSGSGHQSSQDSTWSQANEQNRQRGSASAGASADEDRDKRENRSRQHTNKGWHK
                     GWDNPESSGST"
BASE COUNT          474 a          899 c          893 g          438 t
ORIGIN      
        1 ccacgccctt caccgcggcg atctcctcgc agttcgccac accctgcggc gactcgatca
       61 tcaccacgac gagggtctcc tcgttcacga gcctcgccgc ttcgcccacc ggcgtggagg
      121 cgaagccaag ttgcgcgagc gcgccgccca tcgagcgctt gccgatgggc ggatagcggc
      181 aggaattggc cacatgggaa gcttgttggg agtcgtcgac gtggggaaaa actatgcctt
      241 gcgcgccgtt gtcgagcatg cgcgaggcgt gccagtgctc gaagcccgcg acgcgcacca
      301 ccggcgtcac gccggccgcg agcgaagcgg tggcgatctg cgccacggtg tcgccgtcga
      361 gcgagccgtg ctcgccgtcg atgaagagcc agtcgaaacc ggcggtcttc gccatggtcg
      421 cgatgccgac ggtgcgcgcc tggttgacgc ccatgccgat ggcgagcttg ccggcgcgca
      481 gctggcgcag ggcgtggttg ggaatagcgg tcatagcggt ttcaggtcct cgaggacgaa
      541 gggaaggagc tcgcttacgt tcggatggat gcgaacacgg cgctggaatt cggggtaggg
      601 gagcttggag taaaccgcgt cgatgagcga gtgcacggat tcgtcggcgc tcgtgcccag
      661 gagcgccgcg ccgaggatgc gcttggagct ttcctctacg tggatcttga ggaagccgcg
      721 cgtctcgcct ttctccaccg cgcgcgagac gcgggtcatg ggacgcttgc cgaccagggc
      781 cttgatgccc ttctctttta tctctttctc cgaaaggccg atccggccaa gcggcgggtc
      841 ggtgtagagc gcgtacaccg ggatgcggtc ggtccacttg cggcccgcgt tcgcaagcag
      901 gttgtcggcg acgatctcgt agtcgttgta cgcggtatgc gtgaaagcgc ccttgccgtt
      961 gcagtcgccg agcgcccaga cgttcgggtt cgtggtgcgc agctcctcgt ccacctcgat
     1021 gtagccgcgc tcgtcggtgc ggatgccggc ggcgtccagg cccagatcgt cggtgttcgg
     1081 cacgcgcccg acagcgagca ggagatgcga gccgcgcgca accggcgccc cttcgctgca
     1141 accgaggccg atctccacag cgttctcggt ctttttcgcg gtaagacatt cggcatcgag
     1201 ccgcacctcg acgccttcgg cctcgaggaa ctccttcacc ccggccgaca cgtcctcgtc
     1261 ctcgcgcgca atcagccgcg gcgccatctc gaccacggtg acgcgcgagc cgaagcggcg
     1321 gaacgcctgg ccgaattcca ggccgatgta gcttccgccg accacgatca ggtgctcggg
     1381 cacgaagtcc acgtccatca tcgaaacgtt ggtgagatag ggtacggtgt cgaggcccgg
     1441 catcttcggc acgagcggcc ggccgccgac gtcgatgaag atctgctcgg agacgagggt
     1501 ctgttctccg acctgcaacg tccgcgggcc cgtgaacctg gcgtgtccct ggtaaatcgt
     1561 cccgcccttg agcccgcgca tccacttctc gactccggcg ctcgagctcg agacgaccgc
     1621 gtccttgcgc gccttgacgc gcttcatgtc gacgcggagg ctgccggcga tctccacgcc
     1681 gtagtcgagc gcacgcgagg cgaggtgcgc ggcgtaggcg ctcgccacca tggtcttggt
     1741 aggcgtgcag ccgtcgttca cgcacgtgcc gccgaacttg tgccgctcga tgaccgccac
     1801 cttgcggccg gcgccggcga gccgcgcggc gagcgcgggg ccggcctggc cggtgccgat
     1861 cacaatcgcg tcatagacgg cggcagccac ggccgcacta tatagggccg ggcgcttgta
     1921 gcgttccgcc tacaacaaaa agccgtgccg ggtggcagac aagggcgcgg tgaatccgta
     1981 gagtgaggtc tccccccgag ccattcatcc tccctccctt ggatagtggc tcacccctga
     2041 gaggccgtct tcggacggct tctttttttg tgcccgcgtt tcattcccgc tcgtttcgca
     2101 tggttgaaaa gccctagcga ccccgaggca tgggccttgc ggcaccgcgg taccgcccca
     2161 gacaggggct tgtcaacaca aggaggtatt cggtgcgaaa gctcttaccg gtggttctta
     2221 ccggcgcaat ggccgtttcg ttctccacgc tcgcgcagga cgcggacaag accagggaca
     2281 acgtgcacta caacatccac cagcgcggcg atcagaacac cagccagtac ggctcgggta
     2341 accagagctc gcaggtgtat ggcagcggca atcaggtgaa ccagcagtcc ggcgaaagca
     2401 acgcggcgac ccagacccag cagcaaggct ccgataatac gagcgcgacg accacgcagc
     2461 agggcacgca gaaccagaac gcggtcggca ccggcaacca gcaaagccag cagtccggaa
     2521 gcggccatca gtcgagccag gattcgacct ggtcgcaggc gaacgagcag aaccgccagc
     2581 gaggcagcgc gtccgccggc gcgagcgccg atgaggaccg cgacaagcgc gaaaatcgca
     2641 gccgccagca caccaacaag ggctggcaca agggctggga taacccggaa agctccggca
     2701 gcac
//
LOCUS       DASTPD010000138        10407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1501781, whole genome shotgun sequence.
ACCESSION   DASTPD010000138 DASTPD010000000
VERSION     DASTPD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501781"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFR57_04855"
     CDS             <1..239
                     /locus_tag="VFR57_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU0258737.1"
                     /translation="ILAEAYRDAQNVRGQGDARAAATYAAAFNQNPEFYSFYRSMEAY
                     RSTFRGRNDLMLLETNSDFLRFFRDSLGKPAQRK"
     gene            244..432
                     /locus_tag="VFR57_04860"
     CDS             244..432
                     /locus_tag="VFR57_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEU0258738.1"
                     /translation="MGTTFVMALALMLILEGVLPFLAPNLWRDTFRKITQMSDGQIRF
                     VGLSSMIVGLLILLWTRA"
     gene            429..1370
                     /locus_tag="VFR57_04865"
                     /pseudo
     CDS             429..1370
                     /locus_tag="VFR57_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
     gene            1373..2674
                     /locus_tag="VFR57_04870"
     CDS             1373..2674
                     /locus_tag="VFR57_04870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU0258739.1"
                     /translation="MTARNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHT
                     LVIGGKKTVLHLIPSGILRDSVDCFIGNGVVVSPQALVAEMDELETAGVRSVVARLSI
                     SEACPLILPYHAALDHAREAAKGAQRIGTTGRGIGPAYEDKVARRAIRLQDLFEPARF
                     AQKLRELLDFHNFVLEHYYKQPKLDWREILDQTLALAPRLAPLIADVPRALYHANRAG
                     KNLLFEGAQGSLLDIDHGTYPFVTSSNCVAGAAAAGAGIGPHHLHYVVGITKAYTTRV
                     GGGPFPTELSDDVGEHLRQRGQEFGATTGRPRRTGWFDAAALKRSIQLNGVSGLCITK
                     LDVLDGVDAVKICVGYELEGNFSEILPVGAEELERCHPRYEELPGWMESTVGIKSYEA
                     LPKAARAYLKRIESLAGVPIDLISTGPDRDETIVMRHPYDA"
     gene            2813..3130
                     /locus_tag="VFR57_04875"
     CDS             2813..3130
                     /locus_tag="VFR57_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258740.1"
                     /translation="MYPDIDKARAQHAALRKIVECHVGAADWRTLHRVLDLCRRAAVA
                     IEDRYCRDKLRLVEDFSAEMFSHGESRAPLLKQRILDALDLFASRLYSLETRRRAGTL
                     TSC"
     gene            3465..3662
                     /locus_tag="VFR57_04880"
     CDS             3465..3662
                     /locus_tag="VFR57_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258741.1"
                     /translation="MSKLISAVLAAAFAVATVSPVAFAQTKEEKAAKSKAAVDAACKG
                     KKAGDMVKVDGKDVKCPAPKM"
     gene            complement(3858..3944)
                     /locus_tag="VFR57_04885"
     tRNA            complement(3858..3944)
                     /locus_tag="VFR57_04885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3908..3910),aa:Leu,seq:cag)
     gene            3979..6099
                     /gene="rnr"
                     /locus_tag="VFR57_04890"
     CDS             3979..6099
                     /gene="rnr"
                     /locus_tag="VFR57_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU0258742.1"
                     /translation="MSKKKAKRTALRRRQRSSRDAIDAQRILGALNAARSPLTREELA
                     ARLHVPRHARREFERSLAELERAGDVVQNRAGSLLVSKRLALVAGRVEGHPDGHGFLV
                     PDEGGATIFLTPAEMRQLMHGDRAAVRISGRDLRGRAIGEVVEVLERANRRIVGRLHS
                     EHGVLFLVPEDRRIAHDILVPPAETGRAKAGQVVTVDLVAQPGAHAQPIGRVAEVLGH
                     HADPGMEIEIAVRKFDLPHEFSRKALAQARALPDEVTELENRKDLRELHFVTIDGETA
                     KDFDDAVYARREAKGWRLWVAIADVSHYVRPGDALDRDARERGTSVYFPRRVIPMLPE
                     KLSNGLCSLNPNVERLAMVCEMAITSEGEVARYELYAGVICSRARLTYTEVWERLSSG
                     RAAENLRVLYELFHALFAERSRRGAIDFDTLETRMVFDPRGKIERIVPEPRNDAHRII
                     EECMLAANVCAGNFLASHRQPALYRVHDVPAADKVAALRDFLAELGLQLPGGDIPQPK
                     DYARLLERIRKRPDFGLLQTILLRSLKQAVYSPSNVGHFGLAFEAYVHFTSPIRRYPD
                     LLVHRAIKATLSRKKYVEEDWEALGRHCSETERRADEASRDVENWLKCYYMRDHVGGT
                     FSGSITGVVPFGLFVTLDDYFVDGLVHISELGRDYFQFDPARHMLLGERTGRRYRLAD
                     RMTVKLVRVDLDTRKIDLVPHERA"
     gene            6086..6814
                     /gene="rlmB"
                     /locus_tag="VFR57_04895"
     CDS             6086..6814
                     /gene="rlmB"
                     /locus_tag="VFR57_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU0258743.1"
                     /translation="MKEPRVVFGFHAVLARLRADPKSVVEIFLDETRNDARGKDLVAL
                     AERAGVRVMRVPTKRLDGFYGGGRHQGVVARVEVKNVLDDLNQILEQVDNPLLLVLDG
                     VTDPHNLGACLRVANAAGAHAVIAPRDRAAGITPVVSKVASGAAEATPYLMVTNLART
                     LAELKERNIWVVGAEEGAAKKLYEADLPRSIAWVLGAEGEGMRRLTRESCDLLVRIPM
                     RGEVESLNVAVSAGICLFESVRRR"
     gene            6825..7460
                     /locus_tag="VFR57_04900"
     CDS             6825..7460
                     /locus_tag="VFR57_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258744.1"
                     /translation="MRALVIDDHPLIQEIVPAVLAKALGEVAVATESTLEGGIARAAR
                     EDMPELVVLDLGLPGCQGLEALARFRSKFPEIPLVVLSCTCDRDLILAALEAGAAGYI
                     PKTSKPDVMIAAIRVVAAGGRYVPPEALEDAADKSRPAPRSTAALELTPRQEQVLRLI
                     LKGHNNERIAAELSIACNTVKQHVHAIFTALGVSTRAEAVIAATRHGFSPG"
     gene            complement(7472..7987)
                     /locus_tag="VFR57_04905"
     CDS             complement(7472..7987)
                     /locus_tag="VFR57_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258745.1"
                     /translation="MNYVKWAVACLLLIFGKNLPAVECGPTVKMGEVVGVLGDLRGAR
                     ANLLQHMKSLSSEVQGLSGQAHSRTSQEFDRTSFVDQNAAALEHSLVLVLVLGHLRDG
                     MVDHRDRQRVETSLSVAAAMANDYSAEVQHAADRTLATVGRPGIASDMAKTRDVVQRS
                     AALFAGCTAPR"
     gene            8849..10017
                     /locus_tag="VFR57_04910"
     CDS             join(8849..9107,9107..10017)
                     /locus_tag="VFR57_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611352.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU0258746.1"
                     /translation="MGQAPKRYTPEFRRQMVELHRTGRSLRELSKEFGCTTWSIRTWV
                     KQAARDEGRGDGGLTSGEREELTRLRRENRRLREEREILSKAGGLVCQREGRDTEALF
                     GFVKANQAHHRVTTMCRVLGVSKSGFYAWNERPLSKRGREDIALSAKIHEIHRWSRGV
                     YGAPNVHAELADAHGIKVGCKRVARLMRAAGLKGVMPKRFVTTTVRDDAAATAVDLVD
                     RQFKADGPDQLWVADITYIPTWAGFLYLAVVLDVWSRKIVGWAMATHLRTELVLEALN
                     TALMQRRPLQVVHHSDHGCQYTSYAFGKRCREMNVMPSMGSVGDAFDNAMAESFFATL
                     EKELLDRRRFKTQAEAKLAVFEWIEGWYNPHRRHSSLGRISPINFERRYVTSEAA"
     gene            10180..>10407
                     /locus_tag="VFR57_04915"
     CDS             10180..>10407
                     /locus_tag="VFR57_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0258747.1"
                     /translation="MRLGLIGLGRMGANMARRWIEAGHECVGYARHAVDIEALRCAGF
                     VPATSLEELVGQLTPPRAIWIMIPAAAVDGVI"
BASE COUNT         1851 a         3344 c         3393 g         1819 t
ORIGIN      
        1 tgatcctggc ggaagcctac cgcgacgcgc agaacgtgcg cggccagggc gacgcgcgcg
       61 cagcggcgac ctacgccgcg gcgttcaacc agaaccccga gttctactcg ttttaccgca
      121 gcatggaggc ctaccgctcg accttccgcg ggcgcaacga cctcatgctg ctcgagacca
      181 attccgactt cctgcgcttc ttcagggact cgctaggcaa gcccgcgcag cgcaaatagc
      241 gccatgggaa cgacgttcgt catggcgctc gccctgatgc tgatcctgga gggcgtgctt
      301 cccttcctcg cgccgaatct gtggcgcgac accttccgca agatcacgca gatgagcgat
      361 ggacagatcc gcttcgtcgg cctgtcgtcg atgatcgtcg gcctgctcat cctgctgtgg
      421 acgcgggcat gaagtgggtt ctgccggaat acatcgagga cattcttccg gcgcaggcga
      481 agcgcatcga ggcccttcgt cgcaggatcc tggacctgtt tttctccaaa ggctatgagt
      541 tggtcatgcc gccgctgctg gagtacgtgg attcgctcct gaccggcacc ggccacgacc
      601 tcgagctgcg cacgttcaag gtagtcgacc agctctcggg gcgcatgatg ggggtgcgcg
      661 ccgacatcac gccgcaggtg gcgcgcatcg atgcgcatct tctgaaccgc aggggcgtca
      721 cgcgcctttg ctattgcggc agcgtgctgc acacgcgccc ggccgcgccg ggcgcgacgc
      781 gcgagccgat ccagatcggc gccgagatgt acggcgagcc cggcgtcgcc gccgacgtgg
      841 agatgctcga gctcctctgc cgcgcgctgg agcttgccgg cgtgcgcaag gcgcgcgtcg
      901 acatcggcca cgtcgccatc ttccgcgcga tcgcgcacga ggcgggcgtg aacgccgagc
      961 gcgaggccga gctcttcgag gccttgcagc gcaaggaggt gcccgcgttg agggagcttt
     1021 cgaagaagct gcgcgcgacg atgcgcgagg cgcttctcat gctgccgagc ctttacggcg
     1081 ggccggaagt gctcgacgaa gcgcagaaga agcttccgcc gctcgccgag gtgagaaaag
     1141 cgctcgcgac gctcggccgg ctcgccaaag tgtgcaagtt tcccgccagc ttcgacctcg
     1201 ccgagctgcg cggctaccac taccattcgg gcgtggtgtt cgatgcgtat tgcgatgggg
     1261 taaccggagc ggtggcccga ggtgggcgtt atgatgaagt gggtaaagcg tttggtcgcg
     1321 ctcgaccggc tacgggcttt tccatcgatt tgagagcgct cgcttccgtt tcatgaccgc
     1381 gagaaacgtc gtcgtcatcg gcacgcagtg gggcgacgag ggcaagggca agattgtcga
     1441 ctggctcacc gaccgcgcgc agggcgtggt gcgcttccag ggcgggcaca acgccggcca
     1501 cacgctagta atcggcggga agaagaccgt cctgcacctc atcccttcgg gcatcctgcg
     1561 cgacagcgtc gactgcttca tcggtaacgg cgtcgtggtg tcgccgcagg cgctggtcgc
     1621 cgagatggac gagctcgaga ccgcgggggt gcgcagcgtg gtggcgcgcc tttcgatcag
     1681 cgaggcctgc ccgctcatcc tcccttatca cgccgccctc gaccatgcgc gcgaggccgc
     1741 caagggcgcg caaaggatcg gcaccacggg ccgcggcatc ggcccagcct acgaggacaa
     1801 ggtggcgcgg cgcgcgatcc gtctacagga ccttttcgag cccgcgcgct tcgcgcagaa
     1861 gctgcgcgag ctgctcgact tccacaattt tgtcctggag cactactaca agcagccaaa
     1921 gctcgattgg agggagatcc ttgatcaaac ccttgcgctc gccccgcgcc tcgcgcctct
     1981 gatcgccgac gtgccacgtg ccctctacca cgcgaaccgc gccggaaaga acctgctctt
     2041 cgaaggcgcg caaggctcgc tcctcgacat cgaccacggc acgtaccctt tcgtcacctc
     2101 gtcgaactgc gtcgccggcg ccgcggcggc cggcgcgggc atcggcccgc accacctgca
     2161 ctatgtggtc ggcattacaa aggcgtatac gacgcgcgtc ggcggcgggc cgttcccgac
     2221 cgagctctcg gacgacgtcg gggagcacct gcgccagcgc ggacaggaat tcggcgccac
     2281 caccggccgg ccgcggcgca ccggctggtt cgacgccgcg gcgctcaagc gctcgatcca
     2341 gctgaacggc gtctccggcc tgtgcatcac caagctcgac gtactcgacg gggtcgacgc
     2401 ggtaaagatc tgcgtcggct acgagctcga gggcaacttt tccgagattc tgccggtcgg
     2461 cgccgaagag ctggaacgct gccaccctcg ctacgaggag cttccgggct ggatggagag
     2521 cacggttggc atcaagtcct atgaggcgct acccaaggcg gcgcgcgcgt acctgaagcg
     2581 catcgagtcg ctcgcgggcg tgccgatcga tctgatctcg accgggcccg accgggacga
     2641 gacgatcgta atgcgccatc cctacgacgc ttagcgctat ccaggtcagc tttcacggct
     2701 cccggtggtc ttctttgtca tctcgcaatg acgcgttgtc gggccccgcg cacgcgcttt
     2761 tccgggcctc gctacagcgc ggcggcgatg agcggcgcag aatggccgcg cgatgtaccc
     2821 ggatatcgac aaggcgcgcg cgcagcacgc ggcgctcaga aagatcgtcg agtgccacgt
     2881 cggcgcggcc gactggcgca cgctgcaccg ggtgttggac ctctgccgtc gcgccgcagt
     2941 cgcgatcgaa gatcgctatt gccgcgacaa gctgcgcttg gtggaggatt tctccgccga
     3001 gatgttctct cacggcgaga gccgcgcgcc gcttctcaag cagcgcatcc tcgacgcgct
     3061 cgatcttttc gcaagccgcc tctacagcct cgagacgcgg cgccgcgccg gtacccttac
     3121 cagctgctag tacaatcaga aggttgtcgc gatgatgcct aggaagcgat ccgatcgcga
     3181 tctaagcctc ctccgcatgc cttggaagct ctcgctagcg gcgtagacat cgattcctgc
     3241 tgcgctgcga gtatcgggta gcggcaaagc cgccgatagg cttatttctt agtggcttcc
     3301 gcatggattt gaaatcaact tcaaaagtcg cggcccgggg gtcgggaagg cgcgaaacga
     3361 gacttttttc acgttctggt caaccaactg tcctgctggg tcgttatggg cgttgaccga
     3421 gaactcggtc gtttgctgaa aacactcaac caggaggact acgcatgagc aagctaatta
     3481 gtgctgtttt ggccgccgcc ttcgcagtgg caaccgtttc cccggtcgct ttcgcgcaga
     3541 ccaaagaaga gaaggcagcg aagtcgaagg ccgccgttga cgccgcctgc aaaggcaaga
     3601 aggccggcga tatggtcaag gtggatggca aagacgtaaa gtgcccggcg ccgaagatgt
     3661 aatttcactg tttcgtcctt cgaaaaggga gggctcgcgc cctccctttt tcattgggcg
     3721 ctgcccgctg cccacgcgcc catgacgttt tcgtgtgtcg gagtttttta gttgccgcgc
     3781 tgagcaaagg tgcgtcgcgc cgccatttaa gcgtcggacc aagatcggag cgctattccc
     3841 ggcgggttat attgcggtgg tgccgaggaa gggactcgaa cccccacagt gttgccaccg
     3901 ctaggacctg aacctagtgc gtctaccaat tccgccacct cggcagggga ggcgcgattt
     3961 tagttgcagg tatctgacgt gtcaaagaaa aaagcgaaaa gaaccgcgtt gcggcgaagg
     4021 cagagatcga gccgcgacgc gattgacgcg caacgcatcc tcggcgcgct gaacgccgcg
     4081 cgctcgcctc tcacgcgcga ggagctcgcg gcgcggctgc acgtcccacg ccatgcgcgg
     4141 cgcgaattcg agcgctcgct ggccgagctc gagcgtgcgg gcgacgtggt gcagaaccgc
     4201 gccggcagcc tgctggtgtc gaagcgcctc gcgctcgtcg ccggtcgcgt cgagggccat
     4261 cccgacgggc acggttttct cgtgcccgac gaaggcggcg cgacgatttt cctcacgccg
     4321 gcggagatgc gccagctcat gcacggcgac cgcgccgccg tgcgcatctc gggccgcgac
     4381 ctgcgcggcc gcgccatcgg cgaagtggtg gaagtgctgg agcgcgcaaa ccgccgcatc
     4441 gtcggccggc tgcactccga gcacggtgtg ctgttcctcg tacccgagga ccggcgcatc
     4501 gcgcacgaca tcctggtacc gcccgccgag acgggcagag cgaaggcggg gcaggtggtc
     4561 acggtcgacc tcgtcgccca gcccggggcg cacgcacaac cgatcgggcg cgtcgccgaa
     4621 gtgctcggcc accacgccga ccccggcatg gaaatcgaga tcgcggtgcg caagttcgac
     4681 ctgccgcacg agttttctcg gaaggctctg gcgcaggcgc gcgcgctacc tgatgaggta
     4741 actgagcttg aaaaccgcaa ggacctgcgc gagctgcact tcgtgacaat cgacggggag
     4801 accgccaagg acttcgacga tgcggtgtac gcgcggcgcg aggcgaaagg ctggcgtttg
     4861 tgggtcgcga tcgcggacgt gagccattac gtgcgcccgg gcgacgcgct cgaccgtgac
     4921 gcgcgcgaac gcggcacctc ggtgtacttc ccgcggcggg tgatcccgat gctgccggag
     4981 aaactgtcga acgggctatg ctcgctcaac ccgaacgtcg agcgcctcgc gatggtgtgc
     5041 gagatggcga tcacgtccga gggcgaggtc gcgcgctacg agctctacgc cggcgtgatc
     5101 tgttcgcgcg cccggctcac gtacaccgag gtttgggagc ggctctcctc gggcagagct
     5161 gcggaaaacc tgcgggtgct ctacgagctt ttccatgcgc tcttcgccga gcgcagccgg
     5221 cgcggcgcga tcgacttcga cacgctggag acgcgtatgg tgttcgaccc gcgcggcaag
     5281 atcgagcgca tcgtgcccga accgcgcaac gatgcgcacc gcatcatcga ggagtgcatg
     5341 ctcgcggcca acgtgtgcgc cggcaacttc ctcgcaagcc accggcagcc tgcgctctac
     5401 cgggtgcacg acgtgccggc ggcggacaag gtcgccgcgc tgcgcgattt cctggccgag
     5461 ctgggcctcc agctccccgg cggcgacatc ccgcagccga aggactacgc gcggctcctc
     5521 gagcgcatcc gcaagcggcc cgacttcggc ctgctgcaga ccattctcct gcgctcgcta
     5581 aagcaggcgg tgtacagccc gagcaacgtc ggccacttcg gcctcgcttt cgaggcctac
     5641 gtgcacttca cctcgccgat ccggcgctac cccgatctcc tggtccaccg cgcgatcaag
     5701 gccacgctct cacggaagaa gtatgtcgaa gaggactggg aggcgctcgg ccgccattgc
     5761 tcggaaaccg agcgacgcgc cgacgaagcg agccgcgacg tcgagaactg gctcaagtgc
     5821 tactacatgc gcgaccacgt cggcggcacg ttctccggca gcattaccgg ggtggtgccg
     5881 ttcggcctgt tcgtgacgct ggacgactat ttcgtcgacg gccttgtgca catctccgag
     5941 ctcggtcgcg actacttcca gttcgatccc gcgcgtcaca tgctcctcgg cgagcgtacc
     6001 ggccggcgct accgcctcgc ggaccggatg acggtcaagc tggtgcgcgt ggacctggac
     6061 acgcgcaaga tcgatctcgt tcctcatgaa agagcctaga gtcgttttcg gcttccatgc
     6121 cgtgctcgcg cggctgcgcg ccgacccgaa gtcggtggtc gagatcttcc tcgacgagac
     6181 gcgcaacgat gcgcgcggca aggatctcgt ggcgctggcc gagcgcgccg gcgtgcgcgt
     6241 catgcgcgtt ccgacgaagc ggctggatgg tttctacggt ggcggccgac accagggagt
     6301 cgtggcgcgc gtggaagtga agaacgtgct cgatgacctg aaccagattc tcgaacaagt
     6361 cgacaacccc ctgcttctcg tgctcgacgg cgtaaccgac ccccacaatc tcggtgcctg
     6421 cctgcgagtg gccaatgctg cgggcgcgca tgcggtaatt gcgccgcgcg accgtgctgc
     6481 ggggattact ccggttgtct cgaaggtcgc cagcggcgcc gctgaagcca cgccctacct
     6541 gatggtcacc aacctcgccc gcacgctcgc cgagctgaag gagcgcaaca tctgggtcgt
     6601 gggtgcggag gaaggtgccg ccaagaagct ctatgaggct gatcttcctc gatcaattgc
     6661 atgggtcctc ggtgccgaag gcgagggcat gcggcgcctc acgcgggaaa gctgcgacct
     6721 tctcgtgcgc atcccgatgc ggggcgaggt cgaaagcctg aacgtcgcag tgtcggccgg
     6781 gatctgcctg ttcgagtccg tgcggcggcg gtaacatttc aggcatgcgc gccctggtca
     6841 tcgacgatca tccgttgatc caggagatcg taccggccgt gcttgccaag gcgctcggcg
     6901 aagtggcggt tgcgaccgag tccaccctgg aagggggcat cgcgcgcgcc gcgcgcgagg
     6961 acatgccgga gctggtcgtc ctcgacctcg ggttgcccgg ctgccaggga ctcgaggcgc
     7021 tggcgcgctt ccggtccaag tttccggaga ttccactcgt cgtgctgtcg tgcacctgcg
     7081 atcgcgactt gatcctcgcc gcactcgaag ccggcgcggc cggttacatc ccgaagacgt
     7141 cgaagccgga cgtgatgatc gcagcgatca gagtggtggc cgcaggcgga aggtatgtgc
     7201 cacccgaagc gctcgaggac gcggcggaca agtcgcggcc ggcgccacgc agcacggccg
     7261 cgctcgagct gacgccgcgc caggagcagg tgctgcggct catcctcaag gggcataaca
     7321 acgagcgcat cgccgccgag ctctcgatcg cctgcaacac ggtgaagcaa catgtacatg
     7381 ccatcttcac cgctctcggg gtctcgacgc gcgccgaagc ggtcatcgct gcgacccggc
     7441 acggcttctc accgggctga ttgaatatcg gctaccgagg agcagtgcat ccggcgaaca
     7501 gggcagcgct ccgctgcacc acgtcgcgcg tcttcgccat gtcggacgcg atgcccggac
     7561 ggccgactgt ggctaaggtc cgatccgccg cgtgctgaac ttcagcgctg taatcgtttg
     7621 ccattgcagc ggccaccgac aatgacgtct ctacacgctg tctgtcgcgg tggtcgacca
     7681 ttccatctcg aaggtgaccg aggacgagga ccaggacaag actgtgttcc agagctgcgg
     7741 cgttctggtc aacaaagctc gtgcgatcga actcctggct cgtcctggaa tgcgcttggc
     7801 cgctcagccc ttgcacctcg ctgctgagcg acttcatgtg ttgcagaaga ttagcccgtg
     7861 ctccacgaag atcacctagg acgccgacaa cctcgcccat tttcacagta ggaccgcatt
     7921 ccacagcggg cagatttttc ccgaagatga gaagaaggca cgcaaccgcc catttgacgt
     7981 agttcatcgc tcccctttgc gtcctggcgc gttcgcatcc tgccacaggt gcatcgtttc
     8041 tcgctgaacc gcgccagccg cttttcctta ttgtccagta ccgtaaacaa attcacaggc
     8101 cgatgagcgc tctctgctca ccgctaaacc taaggtcttg atgtgcgcag cattgcgcgg
     8161 caagcctgcg aactcacaac taagatggcc cgccttgtat tgccgatgga acggtccatc
     8221 gaacgacaag cgacttcggg tagtgcggct ttagccaggg agaagaagat gccgctcatc
     8281 ggtggagtag ttcttctaat tcaattctgc tttgcctacc acgctttgaa aacggggcgt
     8341 ccctactggt ggattttcgt gatcatggcg ttcccggttg ctggctgcat catctattac
     8401 ttcgtcgaag tattcccagg atcgcgcgag caacgtaacg ccaatagagc tgcgcgcaag
     8461 cttgcccggg ttctggagcc tgatgctgac ctcaacaagc gcgtcgaaga gctggagatc
     8521 tgcggtagca tcgacaacaa gctcgcgctg gccgccgaat gcatgaatca tcggatgtat
     8581 ccggaagccg caaggcttta cgcgagctgc ctcgatgcag tgtacgcatc cgatggcgcc
     8641 attctctttg gattagccca ggcgctggtc gaaggtggcg attggggcaa ggcgagcgcc
     8701 gccgtcgagc gactcaagtc gaacgcgccg aagttccggc cgctagaggt gcagctgctg
     8761 caagcgaggg ccttggaggg acggggagac aaagtggagt tgacccgttt cagtggacac
     8821 gcaacagtca cactggaagg agtcaaccat ggggcaagcc ccgaagagat acacgccgga
     8881 atttcgccgg cagatggtcg aactgcaccg gactggccga tcgttacgcg agctatcgaa
     8941 ggagttcggc tgcacgacct ggtcgatcag aacgtgggtc aagcaggccg cacgcgacga
     9001 aggtcgaggc gacggcggct taacgagcgg cgagcgcgag gagttaacgc ggctgcggcg
     9061 tgagaaccgc cgcctacgag aggaacgtga aatcctctca aaagccgcgg cctggtttgc
     9121 caacgagaag gtcgcgacac cgaagcgctc ttcggattcg tgaaggcgaa ccaggctcac
     9181 catcgagtga ccacgatgtg ccgggtgctc ggggtgtcga agagcggctt ctacgcatgg
     9241 aacgagcggc cgctgtcgaa gcgggggcga gaggacatcg ctctctcggc caagatccat
     9301 gagatccatc gctggtcccg cggcgtctat ggcgccccga acgttcacgc ggagctcgcc
     9361 gacgctcacg gcatcaaggt cggctgcaag cgcgtggcgc ggctgatgcg cgcggcgggg
     9421 ctgaaaggcg tgatgccgaa gcgcttcgtg acgactacgg tgcgtgacga cgcggcggca
     9481 acggcggtcg atctcgtcga tcggcagttc aaagccgatg gacccgatca gctgtgggtg
     9541 gccgacatca cgtacatccc gacctgggct ggttttttgt acctggcggt cgtgctcgat
     9601 gtctggtcgc gcaagatcgt cggctgggcg atggcgacgc atctgcgcac ggagctcgtg
     9661 ctcgaggcgt tgaacacggc gctcatgcaa cgtcggccgc tgcaggtcgt gcatcacagc
     9721 gatcacggct gtcagtacac gagctacgcg tttggcaagc gctgtcgaga gatgaacgtg
     9781 atgccctcga tgggttccgt cggcgacgca ttcgacaacg ccatggcgga gagcttcttc
     9841 gcgacgctgg agaaagagtt gctcgatcgc cggcgcttca agacgcaagc cgaagcgaag
     9901 ctcgcggtgt tcgagtggat cgagggctgg tataacccgc atcgtcgtca ctcgtcgctg
     9961 ggtcgaatct cacccatcaa cttcgagagg aggtacgtga cgagcgaagc cgcataaccg
    10021 gaaggtcgaa aaccgtccac gaaagcgggt caactccagt actgtcacgc acagagtgct
    10081 acacccggcc ctctagctcg agccctgctg cgcgcagggc gcaacgcttg catggcggcg
    10141 gatgtagcat aggcctcaca cacctcgagg agcgatctcg tgcgactcgg tctcatcggg
    10201 ctcggcagga tgggcgcgaa catggcgcgc cgctggatcg aggccggcca cgagtgcgtc
    10261 ggctacgcgc gccacgccgt agacatcgag gcgctgcgct gcgccggctt cgtccccgcg
    10321 acgtcgctcg aggagctcgt cgggcagctc acgccgccgc gcgcgatctg gatcatgatc
    10381 ccggccgccg ccgtcgacgg cgtcatc
//
LOCUS       DASTPD010000139         7235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_685613, whole genome shotgun sequence.
ACCESSION   DASTPD010000139 DASTPD010000000
VERSION     DASTPD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685613"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..906)
                     /locus_tag="VFR57_04920"
     CDS             complement(334..906)
                     /locus_tag="VFR57_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0258748.1"
                     /translation="MRKICASLSLCLLTACATTQEPAVPPTAFRLTSPGNPDNAMMQP
                     KNAGNFAKNPNCTGQNVSPPLAWTNAPDKTRSFAIIWDDQAGRNGLGVSHAVIYGIPA
                     NVTSFAEGELGGAPSAGRFVPGKNVLGMHWLGPCSPKGNAPQHYVMTLIATTLEPGEL
                     PAGLTRPELLKALEGGKALRAASLVLRFAQ"
     gene            997..1824
                     /locus_tag="VFR57_04925"
     CDS             997..1824
                     /locus_tag="VFR57_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0258749.1"
                     /translation="MKPMRSMRERVSAAEWDVRVNLAACYRLVAKYGMTDLIYNHITA
                     RVPGPEHHILISGYGMLYEEVTASSLIKVDLAGNIIEKPDHGYSVNAAGYIIHSAVHE
                     AREDAQCVVHTHTPAGISVSALEEGLLPLSQTAMRFHGHLAYHDYEGPAFNRGEKQRL
                     VAHLGRHNAMLLRNHGLLVCGPSIPQAFNLIYWLEQACRIQVQTLSCQRPLHEAKPEV
                     VELTAEALSGMEITLDNEAATNPHVKQDAQKAGSGYGLLEWPTLLRSLDRLDRSYQD"
     gene            1901..2524
                     /locus_tag="VFR57_04930"
     CDS             1901..2524
                     /locus_tag="VFR57_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258750.1"
                     /translation="MLRATSASIFLLLALSAGAQTPAQRAPQAPTAKPQKELRAPDVI
                     FVPTPHEVVDAMLKVAKVGKDDVLYDLGSGDGRIPITAAQKYGIARGIGIDINPQRIK
                     EANENLRKARVGERVRFVNADLFESDLSDATVITLYLLPELNLKLLPKLLKEVKPGTR
                     IVSHAFDMGTWKPEQSLDVGGRKVYFWMIPAPGTASYDAAMAAANAR"
     gene            2617..2793
                     /locus_tag="VFR57_04935"
     CDS             2617..2793
                     /locus_tag="VFR57_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258751.1"
                     /translation="MAAEESAKNPFFKKVLESQKSYASLVVSAKRFLFPPYSFAANYY
                     WPEEKPAAKAAKKQ"
     gene            complement(2804..3172)
                     /locus_tag="VFR57_04940"
     CDS             complement(2804..3172)
                     /locus_tag="VFR57_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258752.1"
                     /translation="MLRAGNGNQERAIAGPTDRRSAERRKSDRRSDRALADGGAELIG
                     YRIEAPDGVVGRAADFCIEQEGWAVTGFLAAPRRLFPTRRRIFVPLSAIERIDRRAKT
                     IYVRVGREELARGSRRQPRA"
     gene            3381..3893
                     /locus_tag="VFR57_04945"
     CDS             3381..3893
                     /locus_tag="VFR57_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258753.1"
                     /translation="MRAWLHLLKARMLLFCSLAVVLIGLAPLAYLGGLLAWQIDMQLK
                     TGTWIALPAGLAFVDRALLQGSNLAPVLAFIPQLDSTWTTHDVVWRVLSQLHIGLIPG
                     LIGFGVMAVGISSLLRQATLIRIHKERRKAPVQRLDKHRREAKPVSDDRRREPFIGAG
                     DAANTDRRAA"
     gene            3953..4555
                     /locus_tag="VFR57_04950"
     CDS             3953..4555
                     /locus_tag="VFR57_04950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258754.1"
                     /translation="MRLVTLALAAACVGCESWTGFGFRDEGGEMARLHEVLALDKGKV
                     VADVGAGKGGLTFALAKVVSEGRVFSTEIDPARLRRLQEAVLAAKLANVTVLEAHSSE
                     TGLPANCCDAIVLRRVYHHLTDPSGLNAGLLLALRPGGVLAVIDFPPPFFLGRGQFGV
                     PAGDVTTEVKASGFELVRLIDDWPGRGPFATYCALFRKPL"
     gene            4552..5016
                     /locus_tag="VFR57_04955"
     CDS             4552..5016
                     /locus_tag="VFR57_04955"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0258755.1"
                     /translation="MKPDVPELGLVRPPLVYLASVAIAAVLQFAMPLPFLRGTLNAAL
                     GACLVVIGVALFSYSVAKFRAAGTPVPARKPTIAIVRVGPYSISRNPIYLAFSILQLG
                     IAIWANSLWQLATLAVAVSLINCRVIPREERYLEQKFGAEYRHYKAQVRRWL"
     gene            5057..5935
                     /locus_tag="VFR57_04960"
     CDS             5057..5935
                     /locus_tag="VFR57_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0258756.1"
                     /translation="MAARLTLLGLSVALGALLVAPKHDKSPYMRDDIRIPVPDGHYSL
                     AVTILRPRGEGPFGAIVLNHGVGVGAYERFIESPTLLIQAASAFVARGYVVVMPLRRG
                     FGETGGTFAESPGECSNPHYGRAERAAATDVLAAYEFARKLPYVDPDRMILAGQSAGG
                     VASLYAAAQQPDGLMAVLAFAAGRGADPLGRTGVPCAAEALAALFQDLGVSVRVPVLM
                     YYTENDLYFGPSASRSWFQRLQAGGVRGEYVLQPPFGDNGHFVFSHTKGIPLWLPAVE
                     RFLERHGIPFAPKRAA"
     gene            5972..6448
                     /locus_tag="VFR57_04965"
     CDS             5972..6448
                     /locus_tag="VFR57_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEU0258757.1"
                     /translation="MDFRADLIAQMKNRALIYMEMYDVLVEEFGSARAEALLTKAIYR
                     RGRAAGAALAKYAPADLPGLREAFLAHVPGGSEIFQPELVRADAARLEIQLHGCPLKD
                     AWRDAGLAEDKVATLCRIAGAVDKGLFEGAGFAFENRTWTPGAAGCCFLRVEAKRP"
     gene            6480..>7235
                     /locus_tag="VFR57_04970"
     CDS             6480..>7235
                     /locus_tag="VFR57_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258758.1"
                     /translation="MKRRFAAAAFVVAAIPFAAQAQQKELKVGVIYDYTGAFAAGGSE
                     AAGIGTKIAIDMLNERGGVEGYKINAIYADAQSKTEVAINEATRLLEQENVNLIMGVY
                     SSAHCVPMAQRVDAAKKFMWANVCVASSVFKDKKLSYVFRPQVHSDQFGEASCNFVAE
                     NAKDKLKTDPKSVRVAIIYEDGPYGSGVAQGNEAKCKELGMNIVLKEGYAATAPDLSS
                     LVTKLRRARPDVLLHTGYNPDITLFLRQSREQNL"
BASE COUNT         1284 a         2326 c         2323 g         1302 t
ORIGIN      
        1 gacacaggac gaacgaccga gcgcgaaggt acatggcgag ggtgcgagcg gcaaagctgc
       61 gcctgcgtag gtccctcaac tgcgcttcaa gtgcggacgt gagcctcgcg ttcacttcga
      121 ccagccttac caacgggcgg aagcatttaa cgtaccgcac ggacgttaca cacgagcgtg
      181 cacccgagcg attcaatgcc ctgttcggga ctacctgggc cgactggggc ccctatagtg
      241 gccggctcca ccaattcagc ttcgcctgga gcggccgcat tgctgccgcg aaggcctacg
      301 gctggtcatc caaaaacggc ttgaacgatc tcctcattgc gcgaagcgca gcaccaggct
      361 cgccgcgcgc aacgccttgc cgccctccag cgccttcagt agctcgggcc tagtgaggcc
      421 agccggcagt tcgcccggct caagcgttgt ggcgatgagc gtcatcacgt agtgctgcgg
      481 ggcgttgcct ttgggcgagc atggcccgag ccagtgcatg ccgagcacgt tcttgccggg
      541 aacgaaacgc ccagccgaag gcgccccgcc cagctcgccc tcggcgaagg acgtgacgtt
      601 cgccgggatt ccgtaaatca ccgcatgcga gaccccgagg ccgttgcggc cggcctggtc
      661 gtcccagatg atcgcgaagc tgcgcgtctt gtccggcgcg ttggtccacg cgagcggtgg
      721 cgaaacgttc tggccggtgc agttcgggtt tttggcgaag ttgccggcgt tcttcggctg
      781 catcatcgcg ttgtccgggt tgccggggga cgttaaccgg aaggcggtgg gcggcaccgc
      841 aggctcctgg gtggtggcgc aggcggtaag cagacacagc gagagcgaag cgcagatctt
      901 tctcatgatg cgggcgtcct ttcttgggga tgcgcctcgg tattgaaaca ggccggcgcg
      961 ttggcatcaa caagttctgc gcctaacatt gacagaatga agccgatgcg gtcgatgcgc
     1021 gagcgggtca gcgcagccga gtgggacgtg cgcgtcaatc tcgcggcctg ctatcgcctg
     1081 gtggcgaaat acggcatgac ggacctgatc tacaaccaca tcactgcgcg cgtgccaggc
     1141 cctgagcacc acatcctcat cagcggctac ggcatgctct acgaggaggt cactgcctcg
     1201 agcctgatca aggtcgacct ggcgggaaac atcatcgaga agcccgacca cggctattcg
     1261 gtgaacgctg ccggctacat catccacagc gcagtccatg aagcgagaga ggacgcgcag
     1321 tgcgtggtgc acacgcacac tccggccggc atctcagtgt cggcgctgga agaaggattg
     1381 ttgccgctct cgcagacggc gatgcgcttc catggacacc tcgcatacca cgactacgag
     1441 ggacccgcct tcaaccgcgg cgagaagcag cgcctggtcg cgcacctcgg ccgccacaac
     1501 gcgatgctcc tgcgcaacca cgggctgctg gtatgcggtc cgagcattcc gcaggccttc
     1561 aacctgatct actggctgga gcaggcatgc cgcatccagg tgcaaaccct gtcctgccag
     1621 cgtccgctgc acgaggcgaa gcccgaagtc gtcgagctaa ccgccgaggc actctccggc
     1681 atggaaatta cgctcgacaa tgaagcggcc accaatccgc acgtcaagca ggacgcacag
     1741 aaggctggca gcggctacgg cctgctcgag tggcctactt tgctccgctc gctcgaccgc
     1801 ctcgaccggt cttatcagga ctgaacgaca acggtcggat acgtgcgcaa gcttccgagt
     1861 gttgacacac cccgccttcc atagcgaata ggacacccac atgttacgag ctacatccgc
     1921 ctccattttt ctgctcctcg cgctctccgc cggcgcgcag acgccagcgc agcgcgcgcc
     1981 tcaagcgcct acagctaaac cgcagaagga actgcgcgcc ccggacgtga tcttcgtgcc
     2041 gacgccgcac gaagtggtcg acgcgatgct gaaggtcgcc aaggtcggca aagacgacgt
     2101 gctgtacgac ctcggctccg gcgatggccg cattccgatt actgcggctc agaagtacgg
     2161 cattgcgcgc ggcattggca tcgacatcaa cccgcagcgc atcaaggaag cgaatgaaaa
     2221 cctgcgcaaa gcccgggttg gagagcgggt ccgtttcgtc aacgcggatc tattcgaatc
     2281 ggatctcagc gacgcgacgg taatcacgct ctatctgctg ccggagctga acctcaagct
     2341 gctgccgaag ctgttgaaag aagtgaagcc cggtacacgc atcgtttcgc atgcattcga
     2401 catggggact tggaagccgg agcagtcgct ggacgtaggt gggcgcaagg tttacttctg
     2461 gatgattccg gcgccgggga ccgcttccta tgacgcggcg atggcggctg cgaatgcgag
     2521 gtaaccggtc ggccgtgcta agcattacgg ctgaggcaca ggcctcacac gaagggagga
     2581 gacatcctgg ttgcgttcct caaggcctgg gatcaggtgg cggccgagga atctgcaaag
     2641 aatccgttct tcaagaaggt gctcgagtcg cagaagtcct acgcctcgct cgtggtgtcg
     2701 gcgaagcgat tcctgtttcc gccgtactcg ttcgctgcga actactactg gcccgaggag
     2761 aaacccgccg ccaaggcagc gaagaagcaa tagagctaac cggtcaagcc cgcggctgcc
     2821 ggcgggagcc gcgcgcgagc tcttcgcggc cgaccctcac gtagatggtc ttcgcgcgcc
     2881 gatcgatgcg ctcgatggcg ctcagcggaa cgaagatgcg ccgcctggtc ggaaaaaggc
     2941 ggcgcggtgc ggcgaggaag ccagtcaccg cccagccctc ctgctcgatg cagaagtccg
     3001 ctgcgcggcc gaccacgccg tcaggcgcct cgatgcggta gccgataagc tcggctccgc
     3061 cgtcggcgag cgcgcggtca ctgcgccggt ctgacttccg gcgctctgct gatcttcgat
     3121 ccgttggacc cgcgatggcc cgctcctgat tcccgttgcc ggcccgcagc aatcaccgtg
     3181 ccgcggtcgt taagtcattc ttaagcgcgc cacacgtcca atgcgcccat ggagctgagg
     3241 cgcaaaggtt tgatccggct actcggtcgt atcgagtggg cgaactcgag gtcgtcacgc
     3301 agaacacggc aaagcaatag tccctccgag ctacccggga agccggcagg catgcgccgg
     3361 cgcgcggcat cctttgaggc atgagggctt ggctacacct gctcaaggca aggatgctgc
     3421 ttttctgttc gctggcggtg gtgctgatcg gcctggcgcc gctcgcttac cttggcggcc
     3481 tacttgcctg gcagatcgac atgcagttga agactggcac ctggatcgcg ctcccggcgg
     3541 gtctcgcgtt tgtggatcgt gccctgctcc aaggctcgaa cctggcgccg gtgctcgctt
     3601 tcatcccgca gctcgattcg acgtggacca cccacgacgt tgtctggcgg gtcctgagcc
     3661 agctgcatat cggcctcatt cctggcctca tcggcttcgg cgtcatggca gtgggcatct
     3721 cgagcctgct ccggcaagcg acgttgattc gcattcacaa agagcgcagg aaggcgcccg
     3781 tgcagcgtct cgacaagcac cgccgcgaag cgaaacctgt cagcgatgac agacggaggg
     3841 agccgttcat cggcgcgggc gacgccgcaa atacggatcg ccgcgcggca taaccccccg
     3901 gagggtctta ccctcttgac ttcaagtcga ctttaattgg cacagtgctg ctatgcgctt
     3961 agttacgctt gcgttggcag cggcctgcgt cggatgcgag agctggactg gctttggttt
     4021 tcgcgacgag ggcggcgaga tggcgcggct gcacgaggtt ctcgcgctcg ataaaggcaa
     4081 ggtggtggcg gatgtgggcg ccggaaaggg cgggctcacg ttcgcgctgg cgaaggtcgt
     4141 gtcggagggt cgagtttttt cgactgagat cgaccctgca cgcctgcggc gcctgcagga
     4201 agcggtgctc gcggcgaagc tcgccaatgt gacggtcctg gaagcccact cgagcgagac
     4261 cggattgccc gcgaactgct gcgacgcaat cgtgctgcgc cgcgtgtatc accatctcac
     4321 cgacccgtcc ggcctcaatg ccggcctgct gctcgcgctg cgaccgggtg gcgtgctcgc
     4381 tgttatcgat ttcccgccgc ctttctttct gggccgaggc cagtttggcg tgcccgccgg
     4441 ggacgtgacg acggaagtga aggcgagcgg attcgagctc gttcgcctga tcgatgactg
     4501 gccgggtcgg ggacccttcg cgacctactg cgcgctcttc cgcaagccgt tgtgaagccc
     4561 gacgtaccgg agctcggcct ggttcgcccg ccgctcgtct acctcgcttc ggtcgcaatc
     4621 gcggccgtgc tccagttcgc catgcctttg ccctttctgc gcgggacgct gaacgcggcg
     4681 cttggcgcat gtctcgttgt aattggcgtg gctctgttct cgtattcggt cgcgaaattc
     4741 cgtgcggctg gcacgccggt gccggcacga aagccgacca tcgcgattgt gcgcgtgggt
     4801 ccctatagca tcagccgaaa tcccatttat ttggcgttct ccatccttca gctcggcatc
     4861 gcaatttggg cgaacagcct ttggcagctc gccacgcttg ctgtcgcggt cagcctcatt
     4921 aattgcaggg tcatcccgcg agaggagcgc tatttggagc agaagtttgg cgccgagtac
     4981 cgccactaca aggcccaagt gcgacgttgg ctgtaatcgt gtaagcgaaa cgctcgctgc
     5041 tgcgttattc gactcgatgg ctgcgcgcct cactcttctc gggctatccg tagcgctcgg
     5101 tgcgcttctc gtcgcgccga agcacgacaa gtcgccttac atgcgcgacg acatcagaat
     5161 tcctgtgccg gatggccatt actcgctggc ggtcacgatt cttcgcccgc gcggcgaggg
     5221 gccgttcgga gcgatcgtgc tcaaccacgg cgttggcgtc ggcgcgtacg agcgcttcat
     5281 cgagtcccct actctgctca tccaggcggc gagcgcattc gtggcgcgcg gatacgtcgt
     5341 ggtgatgccg ctgcgccgcg gtttcggcga aaccggcggc acgtttgccg aaagcccggg
     5401 cgagtgcagc aacccgcatt acggccgcgc tgagcgcgcc gccgccacgg acgtcttggc
     5461 ggcctacgaa tttgcgcgca agctgcctta cgtcgatccg gatcgaatga tcctcgccgg
     5521 acaatcggcg ggtggcgtcg cgtccctgta cgcggccgcg cagcagcccg acggcctcat
     5581 ggcggtgctc gccttcgccg ccggacgcgg cgccgatccg ctgggccgta ccggcgtgcc
     5641 gtgcgcggcg gaagcgctgg cggcgctgtt tcaggatctc ggcgtgtccg ttcgggttcc
     5701 ggtcttgatg tactacaccg agaacgatct ttacttcggc ccgagcgcct cccgctcatg
     5761 gtttcaacgg ttgcaagcgg gcggcgtgcg cggcgaatac gttctgcagc cgcctttcgg
     5821 cgacaacggt cacttcgtct tcagccacac aaagggcatc ccgctctggc ttcccgccgt
     5881 ggagcgcttt ctcgagcgtc acggcattcc tttcgcgccc aagcgggcgg cgtaacgtca
     5941 ggcgctaaga tctgcagtac gaggaggcca catggacttt cgcgccgacc tgatcgcgca
     6001 gatgaaaaac cgcgcgctca tctacatgga gatgtacgac gtgctcgtgg aggaattcgg
     6061 atctgcgcgc gccgaagcgc tgctcaccaa ggcgatctac cggcgagggc gcgccgcggg
     6121 agcggcgctc gcaaaatacg cgccggcgga cctcccaggc ttgcgcgaag cattcttagc
     6181 ccacgtcccg ggtggaagcg agatcttcca gcccgagctg gtgcgcgccg acgccgcgcg
     6241 cctggagatt cagcttcacg gctgcccgct caaggatgcc tggcgcgacg ccgggctcgc
     6301 cgaggacaag gtggcgaccc tctgccgcat cgccggcgcc gtcgacaagg ggctgttcga
     6361 gggggccggt ttcgccttcg agaaccgcac ctggaccccg ggcgccgcgg gttgctgctt
     6421 cctaagggtg gaagcaaagc ggccctgata cactcgccgg cgaggaggag ctcaccatga
     6481 tgaagcgcag gttcgcggcc gcggcctttg tcgtcgcggc gattccgttc gccgcccagg
     6541 cgcagcagaa ggaactcaag gtcggcgtca tctacgacta caccggagcc ttcgccgccg
     6601 gaggttcaga agccgccggc atcggcacca agatcgcgat cgacatgctg aacgaacgcg
     6661 gcggcgtcga gggctacaag atcaacgcca tctacgccga cgcgcagtcg aagaccgagg
     6721 tggcgatcaa cgaggcgacg cgcctcctcg agcaggagaa cgtgaacctc atcatgggcg
     6781 tctattcgag cgcgcactgc gtaccgatgg cgcagcgcgt ggacgcggcg aaaaagttca
     6841 tgtgggcgaa cgtctgcgtc gcttccagcg tgttcaagga caagaagctc tcctacgtct
     6901 tccgtccgca ggtgcactcc gaccagttcg gcgaggcttc ctgcaacttc gtcgcggaga
     6961 acgccaagga caagctgaag accgatccga aaagcgtgcg cgtcgcgatc atctacgaag
     7021 atgggccgta cggcagcggc gtggcgcagg gcaacgaggc gaagtgtaag gagctcggca
     7081 tgaacatcgt gctgaaggag ggctacgccg ccacggcgcc cgacctctcg agcctcgtca
     7141 ccaagctacg ccgcgcacgg cccgacgtgc tgctgcacac cgggtacaac ccggacatca
     7201 cgctcttcct gcggcagtcg cgcgagcaga atctc
//
LOCUS       DASTPD010000140         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_482419, whole genome shotgun sequence.
ACCESSION   DASTPD010000140 DASTPD010000000
VERSION     DASTPD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482419"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2605
                     /locus_tag="VFR57_04975"
     CDS             <1..2605
                     /locus_tag="VFR57_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0258759.1"
                     /translation="GPDLLTVKVCDGVATEVEPNFAAADVHPGGGKVCVKAYGLVQKA
                     YHPERVLQPMKRTNPRKGRNEDPGFVPISWEEALSLVADKLNEIRSKGLLDESGYPRV
                     AASFGGGGTPQFYMGSFPAFLAAWGAVDMGFGSGQGVKCYHSEHLYGELWHRGFTVSP
                     DTPLTNYLISCGANVEASGGVAGVWRHANARSRGMKRVQVEPHLSVTGACSAEWVPIR
                     PKTDAAFLYALIYVLLHEETRSRLDLEFLYRRTSSPYLVGANGYYLRDPGYGQPIVAG
                     SLETKETHVALEIGLDGDVLAEGRLEGETAFAKLLAHMKPYSPEWAQAICDVPAATIR
                     RIAREYLDAARVGETIAIEGKRLPYRPVAVSLGKTVTNGWGGYECCWARTLLASLVGA
                     LEVPGGILGTTVRLNRPMSERHRSVKAGPDGLMNYPFNPTDAAHWSAQPNIRNAYRSM
                     VPLAADGPWSQALGPTHFSWMFLDETPPGLPRVTLPELWFLYRTNPAISFWDNAALEK
                     RMARFPLVVAFAFTRDETNHFADILLPDAGDLESLQLIRIGGSKYIEQFWDHEGFALR
                     QPAIAPRGQARDFTDIATDLAARCGLTEGYNGAINRGTLGVPLKEFSLEATKSFSSEQ
                     IWDAVCKAASSELTGGAQAHGLDWWKEHGLATRPFPRTAWYLYPTLAAKGLRFEMPYQ
                     ERLLRVGGELGRRLHEKGMKWWDKQLAEYQALPAWKDFPGEWEASLAAMGGEGKDYPF
                     WLLTARSMQYAWGGNVGMQLIKEVADNVAGHGGVILNSSKAKELGIGQGDWVRIATPK
                     AEVRARAVLRQGIRPDTLLLLGQFGHWVTPFAKDFEFPSMNPLVPMSLELTDATGSGA
                     DLVRVALRKAA"
     gene            2602..3249
                     /locus_tag="VFR57_04980"
     CDS             2602..3249
                     /locus_tag="VFR57_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0258760.1"
                     /translation="MTRWAMVADLRRCVGCQTCTAACKQANATPPGVQWRRVVDLEVG
                     EYPDVKRAFVPVGCMHCDDPPCMHVCPSTATRKRPDGVVTIDYDLCIGCGYCAVACPY
                     QARFKVDKAAFAYGGATESETQTFHPEQLGVATKCTFCVERIDAGVAKALKPGVDPEA
                     TPACVNSCIAQALHFGDVEDPESNISQLLAENQFFRMHEELGTGPRFYYLWDKAR"
     gene            3246..3980
                     /locus_tag="VFR57_04985"
     CDS             3246..3980
                     /locus_tag="VFR57_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258761.1"
                     /translation="MNWLQRHWDARAAANFMLGGAGSGLMVAALLVADPSILIIISLI
                     LVASGLTAVWLEIGRKLRAVHVFFNPFTSWMTRESFAALLLFPLGVGALYLPSALPFA
                     AAAAAVFLYCQARILLASKGIPAWRAPAVVPLILSTGLAEGAGIALLFASNNVLLGVF
                     VVAVLGRAAAWWRYRGAVRSSALEPAGRVLVWIGTGAALALVFAPALAGLAALAAGLW
                     LKFALVTRASFNQGFSLPHLPVRGSR"
BASE COUNT          643 a         1338 c         1320 g          698 t
ORIGIN      
        1 gggccccgac ctcctgacgg tcaaagtgtg cgatggcgtg gcgaccgaag tcgagcccaa
       61 tttcgccgcc gccgacgttc accccggcgg cggcaaggtt tgcgtcaaag cgtacgggct
      121 ggtgcaaaag gcctaccacc cggagcgcgt gctacagccg atgaagcgca cgaacccgag
      181 gaaggggcgc aacgaggacc ccggcttcgt cccgatctcg tgggaggaag ctttgtctct
      241 tgttgcagat aagttaaatg aaataagaag caaaggcctg ctcgacgagt cgggctatcc
      301 gcgcgtcgcg gcgagcttcg gcggcggcgg cacgccgcag ttctatatgg gaagcttccc
      361 cgccttcctc gccgcctggg gcgcggtgga catgggcttt ggctcgggcc agggagtgaa
      421 gtgctaccac tccgagcacc tctacggcga gctctggcac cgcggcttca ccgtctcgcc
      481 cgacacgccg ctcactaact acctgatctc ctgcggggcg aacgtcgagg cttcgggcgg
      541 ggtggccggg gtctggcgtc acgcgaacgc gcgcagccgc ggcatgaagc gcgtgcaggt
      601 cgagccgcac ctgtcggtca ctggcgcctg ctccgccgaa tgggtaccaa tccggccgaa
      661 gaccgatgcc gcgttcttgt acgccctcat ctatgtgctg ctgcacgagg agacgcgcag
      721 ccgcctggac ctcgaattcc tctatcgccg cacctcctcg ccgtatctgg tcggggcgaa
      781 cggctactac ctgcgcgatc cgggctacgg ccagccgatc gtcgccggct cgctcgagac
      841 gaaggagacg cacgtcgcac tcgagatcgg cctggacggc gacgtgctcg ccgaaggtcg
      901 gctcgaaggc gagaccgcgt ttgccaagct cctcgcgcac atgaagcctt actcgcccga
      961 atgggcgcag gcgatctgcg acgtgcccgc ggcgaccatc cggcgcatcg ctcgtgagta
     1021 cctcgacgca gctcgcgtcg gagaaacgat cgcgattgaa ggaaaacgtc tgccgtaccg
     1081 gccggtcgcg gtgagcctgg gcaagacggt gactaacggc tggggcggct acgagtgctg
     1141 ctgggcacgc acgctgctcg cctcgctcgt aggagcgctc gaagttccgg gcggcatcct
     1201 cggcaccacg gtgcggctca accgcccgat gtcggagcgc caccgcagcg tgaaggcggg
     1261 ccctgacggg ctgatgaact acccgttcaa cccgactgac gccgcgcact ggagcgcgca
     1321 gccgaacatc cgcaacgcgt atcgctcgat ggttcctctc gccgccgacg gcccctggag
     1381 ccaggcactc ggcccgacgc atttctcgtg gatgttcctc gatgagacgc cgccgggact
     1441 gccgcgcgtg acccttcccg agctctggtt tctctaccgt accaacccgg cgatctcgtt
     1501 ctgggacaac gccgcgctcg agaagcgcat ggcgcgtttc cccttagtcg tcgcgttcgc
     1561 ctttacccgc gacgagacca accatttcgc cgacatcctg cttcccgatg cgggcgacct
     1621 ggagagcctg cagctcatcc gcatcggtgg ctcgaaatac atcgagcagt tctgggacca
     1681 cgaagggttc gcgctcaggc agccggccat cgcgccgcgc gggcaggccc gcgatttcac
     1741 cgacatcgcg accgacctcg ccgcgcgctg tggcctcacc gagggctaca acggcgccat
     1801 caaccgcggc accttgggcg ttcccttgaa ggagttttcg cttgaagcga caaaatcctt
     1861 ctcctcggag cagatctggg acgcggtgtg caaggcggca agcagcgagc tgaccggggg
     1921 cgcccaggcc cacgggctcg actggtggaa agagcatggc ctcgctaccc gacctttccc
     1981 gcgcaccgcc tggtatctct acccgacgct tgcggccaag ggcctacgtt tcgagatgcc
     2041 ctatcaggag cgcttgctgc gggtaggagg cgagctggga cgccggctgc acgagaaagg
     2101 catgaagtgg tgggacaagc agctcgccga gtaccaggcg ttgcccgcgt ggaaggactt
     2161 tccgggcgaa tgggaagcct cgctcgccgc tatgggcggc gagggcaagg attacccgtt
     2221 ctggcttctc accgcccgct ccatgcagta cgcgtggggc ggaaacgtcg gcatgcagct
     2281 catcaaggaa gtcgcggaca acgtcgctgg ccatgggggc gtgattctta attcttcaaa
     2341 agctaaagag ctgggcatcg gccaaggcga ttgggtgcgg atcgccacgc caaaggccga
     2401 agtgcgtgcg cgcgccgtgc tgcgccaggg aatccgcccc gacacgctgc tcctgctcgg
     2461 ccaattcggc cactgggtga cgccgttcgc caaggacttc gagttcccgt cgatgaaccc
     2521 gctcgtgccg atgtcgctcg agctcacaga tgccaccggc tccggagcgg atcttgttcg
     2581 agttgccctg aggaaagctg catgacgcgc tgggccatgg tcgcggatct gcgccgctgc
     2641 gtcggctgcc agacgtgcac ggcggcgtgc aagcaggcga atgcgacgcc gcccggcgtg
     2701 caatggcggc gcgtggtcga cctcgaggtc ggcgagtatc cggatgtgaa gcgcgcgttc
     2761 gtgccggtgg gctgcatgca ctgcgacgac ccgccttgca tgcacgtgtg cccgagcacc
     2821 gctacgcgca agcggcccga cggcgtcgtc accatcgatt acgacctctg catcggctgc
     2881 ggctactgcg ccgtcgcctg tccttatcag gcgaggttca aggtggacaa ggcggcgttt
     2941 gcctacggcg gcgcgacgga gagcgaaacg cagactttcc atcctgaaca gctcggggtc
     3001 gccaccaagt gcaccttttg cgtcgagcgc atcgatgccg gcgtagcgaa ggccctgaag
     3061 ccgggcgtgg acccggaggc gaccccggca tgcgtgaatt cctgcatcgc ccaggcgctg
     3121 cacttcggcg acgtggagga tcccgagagc aacatctcgc agctcctcgc cgagaaccaa
     3181 tttttccgca tgcatgaaga gctcggcacc gggccgcgtt tctactactt gtgggacaaa
     3241 gcgcgatgaa ctggctgcag cgccactggg atgcccgcgc cgccgccaac ttcatgctgg
     3301 gcggcgccgg gtcggggctg atggtggcgg cgctcttagt cgccgatccc tcgatcctga
     3361 tcattatttc tctcatcctg gtcgcgagcg gcctgacggc agtttggctc gagatcggcc
     3421 gcaagctgcg cgcggtgcac gtgttcttca atcctttcac ctcgtggatg acccgcgagt
     3481 cgtttgccgc cctgctcctc ttcccgctcg gcgtcggcgc gctttacctg ccctccgcgc
     3541 tgccgttcgc agccgccgcc gcggccgtgt tcctctactg ccaggcgcgc attctgctgg
     3601 cctcgaaggg aatcccggcg tggcgggcgc ctgcggttgt gccgctcatc ctttcaaccg
     3661 ggctcgccga gggagcgggc atagcgctct tgtttgccag caacaacgtc ctcctgggcg
     3721 tgttcgtcgt cgcagtcctc ggtcgcgccg ccgcctggtg gcgctatcga ggggccgtga
     3781 ggtcgagcgc gctcgagcct gccggcagag tgctcgtatg gatcgggacc ggcgccgcgc
     3841 ttgcgctggt gttcgcgccg gcgctcgccg gcctcgccgc gctcgcggcg ggcttgtggc
     3901 tcaaattcgc gctcgtcacg cgcgcatcgt tcaaccaggg attctcgctg ccgcacctgc
     3961 cggtgcgggg aagccgatag acaggggata gagacgcca
//
LOCUS       DASTPD010000141         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_73730, whole genome shotgun sequence.
ACCESSION   DASTPD010000141 DASTPD010000000
VERSION     DASTPD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73730"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFR57_04990"
     CDS             <1..345
                     /locus_tag="VFR57_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0258762.1"
                     /translation="MKLGMRIDYTLDWKRVAPPLRNDTKILESLLKSARASIGAQNVY
                     ELPEPSMGSEDFAWFAERVPAAHLRIGSKIDGLDTAIHRSNYDCNELAIPVGVKVLAR
                     AALDLGKSGTEP"
     gene            complement(373..516)
                     /locus_tag="VFR57_04995"
     CDS             complement(373..516)
                     /locus_tag="VFR57_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258763.1"
                     /translation="MDRKRNSERPQPLEHPIEPEMDKRPEDSEHGRDPATEPPKDKRT
                     DNL"
     gene            556..966
                     /locus_tag="VFR57_05000"
     CDS             556..966
                     /locus_tag="VFR57_05000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0258764.1"
                     /translation="MKWFDLMRTVVLPAHCDRYGHMNVRQYAAFFDDAGWHIPAMAGI
                     SLQEVWARGLGTVMASLTIDFHHEITSGQLVLVRGAVTKVGTKSFAHEMRLYEADSMT
                     HCATQKSVEVCFDTKARKGVALPDDLRNKLEAIL"
     gene            complement(956..1906)
                     /locus_tag="VFR57_05005"
     CDS             complement(956..1906)
                     /locus_tag="VFR57_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258765.1"
                     /translation="MKKILAVLLLLPLLAFAQSYPSKPVRLIVGFPAGGPADIFGRAL
                     AQGMAAELGQPVVVENVAGVGGVLGVDRAAKAAPDGYTLCINSASPLVIAPYSLSKNP
                     FDVKKDFAFLTLVVRVPEVLAVHPALPVASLSELVAYAKANPGKVNFGSAGSGSITHL
                     AGELLKSEAKVDLVHVPYKGAAPAVNDLLGGQVQMGIFDVPVLLGHIRSGKLKALAIT
                     SASRAPSLPDVPTTGELSYPNVNSDNWYGLVAAAATPPEVQKRIHAAAIAALKSKAVE
                     EQFSKVGGIASPGTPQEYQAFIAAEQAKWSRIVQAIGFKE"
     gene            complement(1903..2757)
                     /locus_tag="VFR57_05010"
     CDS             complement(1903..2757)
                     /locus_tag="VFR57_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0258766.1"
                     /translation="MSYHNNPTEPKLKLPAGACDAHFHVFGPKDKFPYAKDAPYVPKV
                     DAPKDVLIDRHMFLGIERGVVVQSAAHGYDNSAAADLLAEKKGSYVGVALVPVNTDIS
                     ELKKLHAQGFRGVRFNFLQRLGGGPAVADVMRFATKLAVLGWHLQLHVEPQHILPMTP
                     ELKLSPVPVVIDHMGRVDASRGLDQPGFTHLLKLMEDQRFWVKVSGLERASRQGAPYA
                     DAVPFARKLVADFGSRVLWGSDWPHPNLDSVPDDGQLVDLIGEFANEMQRQALLVDNP
                     QKLYKFES"
BASE COUNT          457 a          925 c          973 g          498 t
ORIGIN      
        1 atgaagctcg gcatgcgcat cgactacacg ctcgactgga agcgcgtggc gccgccgctc
       61 cgtaatgaca cgaagatttt ggagtccttg cttaagagcg cacgggccag tatcggtgcg
      121 cagaacgtct atgagctccc cgagccatcg atggggagcg aggacttcgc ctggttcgcc
      181 gagcgcgtgc cggccgcgca cctgcgcatc ggctccaaga tcgatggcct cgacaccgcg
      241 atccaccgct cgaactacga ctgcaacgag ctggccatcc cggtgggggt caaagtcctc
      301 gcgcgcgcgg cactcgattt ggggaaatcg gggacggagc cctaactgat tcgttagggc
      361 tccgtccccg aatcagagat tgtcggtgcg cttgtccttg ggcggctcgg tcgccgggtc
      421 gcggccgtgc tcggaatcct cgggccgctt gtccatctcc ggctcgatcg ggtgctccag
      481 gggctgcggt ctttcggaat tgcgcttgcg gtccatgccc gcgccacgca atcatcgtgc
      541 caaatggcaa acgagatgaa atggttcgac ctgatgcgca ccgtggtgct gcccgcgcac
      601 tgcgaccgct acgggcacat gaacgtgcgc cagtacgccg cgttcttcga cgacgcgggc
      661 tggcacatcc cggcgatggc gggcatctcg ctgcaggagg tgtgggcgcg cgggctgggc
      721 accgtgatgg cctcgctgac catcgacttc caccacgaga tcacgtcggg acagctggtg
      781 ctggtgcgcg gcgcggtgac gaaggtcggc acgaagagct tcgcccacga gatgcggctc
      841 tacgaggcgg actccatgac gcactgcgcg acgcagaagt cggtcgaggt gtgcttcgac
      901 acgaaggcgc gcaagggcgt cgcgctgccc gatgacctgc gcaacaagct cgaggctatt
      961 ctttgaagcc gatcgcctgc acgatgcgcg accacttggc ctgctcggcg gcgatgaagg
     1021 cctgatattc ctgcggcgtg cccggcgagg cgatgccgcc caccttcgag aactgctcct
     1081 ccacggcttt ggatttgagc gccgcgatcg cggcggcgtg gatgcgcttc tgcacttccg
     1141 gcggcgtggc ggccgcggcg accagcccgt accagttgtc cgagttgacg ttcgggtagc
     1201 tgagctcccc cgtggtcggc acgtcgggca gcgatggggc ccggcttgcg ctggtgatcg
     1261 cgagcgcctt gagcttgccg ctgcggatat ggccgagcag caccggcacg tcgaagatgc
     1321 ccatctgcac ctggccgccg aggagatcgt tcaccgccgg tgcggcaccc ttgtaaggga
     1381 cgtgcaccag gtccaccttg gcctccgact tgagcagctc gcccgcgagg tgcgtgatgc
     1441 tgccgctgcc cgccgagccg aagttcacct tgcccgggtt tgcctttgca taggcaacca
     1501 actcactcaa gcttgccacc ggcaatgccg gatggacggc gagcacttcc ggtacgcgga
     1561 ctacgagcgt gaggaaagcg aagtccttct tcacgtcgaa cgggtttttc gagagcgagt
     1621 agggcgcgat gacgagcggc gaggcgctgt tgatgcatag cgtgtagccg tccggcgcgg
     1681 ccttggcggc gcggtcgaca ccgagcacgc cgcccacgcc ggccacgttc tccaccacga
     1741 ccggctggcc gagctcggcc gccatgccct gcgcgagcgc gcggccgaag atgtcggccg
     1801 gcccgccggc cgggaagccg acgatgagcc gcaccggctt gctcggatag ctttgcgcga
     1861 acgcgaggag gggaagaagc agcagcacgg cgaggatttt tttcatgact cgaacttgta
     1921 gagtttctgc gggttgtcga ccagcagcgc ctggcgctgc atctcgttcg cgaactcgcc
     1981 gatcaggtcg acgagctggc cgtcgtcggg caccgagtcg agattcgggt gcggccagtc
     2041 gctgccccac agcacgcggc tgccgaagtc ggcgaccagc ttgcgcgcga acggcaccgc
     2101 gtcggcgtac ggcgcgccct ggcgcgaggc gcgctcgagg ccgctcacct tgacccagaa
     2161 gcgctggtcc tccatgagct tgaggaggtg cgtgaagccg ggctggtcga ggccgcgcga
     2221 ggcgtcgacg cggcccatgt ggtcgatcac caccggcacc ggcgagagct tgagctccgg
     2281 cgtcatcggc aggatgtgct gcggctcgac gtgcagctgc aggtgccagc cgagcacggc
     2341 gagcttggtg gcgaacctca tcacgtccgc gaccgcggga ccgccgccga gccgctgcag
     2401 gaaattgaag cgcacgccgc ggaagccctg cgcgtgcagc ttcttcagct ccgagatgtc
     2461 ggtgttgacc ggcaccagcg ccacgccgac gtagctgccc ttcttttccg cgagcaggtc
     2521 ggccgccgcc gagttgtcgt agccgtgcgc ggcggactgc accaccacgc cgcgctcgat
     2581 gccgaggaac atgtgccggt cgatcagcac gtccttcggc gcatcgacct tcggcacgta
     2641 cggcgcgtcc ttcgcgtatg gaaacttgtc cttcgggccg aacacgtgga agtgcgcgtc
     2701 gcacgcgccg gccggcagct tgagcttcgg ttcggtcgga ttgttgtgat agctcatgat
     2761 gcgacgcgat tctatgcgat gaagccgatg cggcgcttgg gtaccgcttt acggccattg
     2821 tcttctgcga tcagccgctt gatagcatca aag
//
LOCUS       DASTPD010000142         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1504368, whole genome shotgun sequence.
ACCESSION   DASTPD010000142 DASTPD010000000
VERSION     DASTPD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504368"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1352
                     /locus_tag="VFR57_05015"
     CDS             165..1352
                     /locus_tag="VFR57_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258767.1"
                     /translation="MKNDANVIGLISFAHGMSHFYQLLLAPLFPFIKDELGVSYAALG
                     FLVALFYTLSGLLQPLAGFVVDRYGARSVLLAGVSFFVAATLVMGLSTSYAMLALGAA
                     LGGIGNSVFHPADFAILNARVSSTRLGYAFSAHGVVGYVGYAVAPVFSVAIGAALGWQ
                     AALLAGAVLGVGLLLLLLLNSRHLVIGAQPRKKASLKTEAGVLFAAPVVLCFVYFTVF
                     AAGLAGLQSFGVSAMVEQYRIAATSASSALTAYLVSAALGILAGGFVAARTTRHDFVA
                     AAGMGVSALAILAVAMNVVPGVALAAALAISGFASGVVAPSRDLIVRASTPPGAAGRV
                     FGFVYSGLDVGAFATPVFYGWMLDHGMPHAVFYTIFVFTLAAMLTVLLVRVANPLSVQ
                     HPQ"
     gene            complement(1308..2087)
                     /locus_tag="VFR57_05020"
     CDS             complement(1308..2087)
                     /locus_tag="VFR57_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0258768.1"
                     /translation="MSANPKFKSTPVRERVSKEEWQVRVDLAAAYQLAAIFKWTDLIY
                     THFSARVPGTEDFLINPYGMMFDEISASNLVKIDWEGKILDDPLDMGVNEAGFVIHSC
                     MHAARPEINCVIHTHTRAGVAVSAMKCGLLPISQHAMMVQADITYHDYEGIALYKEEQ
                     QRMAADLGKTSKAMILRNHGLLALGQTVREAFEVMYYLDAACQIQVDACAGGMHNVHL
                     MSEQAAKTAATQFKREGRPAHYKDWPALLRMLDRKGIRYAD"
     gene            complement(2137..3075)
                     /gene="dusA"
                     /locus_tag="VFR57_05025"
     CDS             complement(2137..3075)
                     /gene="dusA"
                     /locus_tag="VFR57_05025"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEU0258769.1"
                     /translation="MSHGISVAPMMDVTDRHCRFFLRQVNSRARLHTEMITTGALLHG
                     DVPRHLEFNSEEHPVALQLGGSEPAELAHCAKLGEQYGYDEINLNIGCPSERVQRGAF
                     GACLMAEPQLVGTCVKAIRDAVQIPVTVKHRVGIDKVEDYSFVRDFVGTVARAGCRTF
                     IVHARNAVLKGLSPKENREIPPLKYDYVYRLKRDFPGLEIVVNGAIASKEQIQEHLRR
                     VDGVMIGRAAYSNPWFLADPGKTRRDVVRVMCGYAKNVDSLRHVTRHMLGLYHGHPRA
                     KLWRRMLSDPVELKRNDPELLLRAFDVVEDRIAQAA"
     gene            complement(3079..3714)
                     /locus_tag="VFR57_05030"
     CDS             complement(3079..3714)
                     /locus_tag="VFR57_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEU0258770.1"
                     /translation="MLGALAAFSVNAQYNGPAVETCRAYAKREMAREGTGGKDIVIER
                     DQALSIERYTRKLGNQLVSSILRGNGAVVLDNAPSAELAFICLLADDKRAVFFQWLPR
                     PNASALAQCTRDTALREQPRPCLQTLLQVAENDLMQSYALLFQEARERDHSAGNEKAV
                     DTFRKANEEWRQYRDAECARRRDHTPTGVSQEDHHLACFIELTRRRALDMK"
BASE COUNT          592 a         1261 c         1266 g          765 t
ORIGIN      
        1 cacgacgacg agtacggtac gatggcgcag cttgacgaga tcgcgcgccg cgtgagcggt
       61 ccgtgcgagc tcctcaagct cgagaattgc ggccactcgc cgttcaggga ccagcccgaa
      121 accgtgctgg ctgcaacagt ccgattcata gaacggctct tttcttgaag aacgacgcga
      181 acgtcatcgg gctcatctcg ttcgcgcacg gcatgagcca tttctaccag ctcctgctcg
      241 cgccgctgtt cccgttcatc aaggacgagc tcggagtctc gtacgcggcg ctcggcttcc
      301 tggtcgcgct gttctacacg ctttccgggc tgctgcagcc gctcgcgggg ttcgtcgtag
      361 accgctatgg cgcacgcagc gtgcttctgg ccggtgtctc cttcttcgtc gccgctacgt
      421 tggttatggg tctttcgacc agctacgcaa tgctcgcgct cggcgcagcg ctcggcggca
      481 tcggcaacag cgtgttccat cccgcggact tcgcgatcct caacgcgcgc gtcagcagca
      541 cgcgcttggg ctatgccttc agcgcgcacg gcgtagtggg atatgtcggc tacgcggtgg
      601 cgccggtgtt cagcgtcgcg atcggcgcgg cgctcggctg gcaagcggcg cttctcgccg
      661 gcgcggtgct cggcgtcggg ctgctgctac tgctgctctt aaactcgcgg cacctcgtga
      721 tcggagcgca accgagaaag aaggcgtcgc tcaagaccga agcaggcgtg ctgttcgccg
      781 cgccggtcgt gctgtgcttc gtgtacttca cggtattcgc cgcggggctc gccggtctgc
      841 agagcttcgg cgtttccgcc atggtcgagc agtaccgcat cgcggcaacc agcgcgtcga
      901 gcgcgcttac cgcgtacttg gtgagcgctg cgctcggcat cctcgccggc gggttcgtcg
      961 ccgcgcgcac cacgcgccac gacttcgtgg cggccgcggg gatgggggtc agtgcattgg
     1021 cgatcctggc agtcgcgatg aacgtagtcc caggggttgc gctggcggcc gcgctcgcga
     1081 tctcgggatt tgcctccggg gtggtggcac cctcgcgcga cctcatcgtg cgtgcgtcga
     1141 cgccgccggg cgcggcgggg agggtgttcg gcttcgttta ttcagggctc gacgtgggcg
     1201 cgtttgccac gccggtgttc tacggctgga tgctcgacca cggcatgccg cacgctgtgt
     1261 tctacaccat cttcgtcttt acccttgccg ccatgctgac ggtgctgcta gtccgcgtag
     1321 cgaatcccct ttcggtccag catccgcagt agcgccggcc agtccttgta gtgcgccgga
     1381 cggccttcgc gcttgaactg cgtggctgcg gtcttcgccg cctgctcgga catcaggtgc
     1441 acgttatgca tgccgccggc gcaggcgtcg acctggatct ggcaggcggc gtcgaggtag
     1501 tacatgacct cgaacgcctc acgtaccgtc tggccgaggg cgagcagtcc atgattgcgc
     1561 aggatcatcg ccttggaagt cttgccgagg tccgcggcca tgcgctgctg ctcttccttg
     1621 tagagcgcga tgccctcgta gtcgtggtac gtgatgtcgg cctgcaccat catcgcgtgc
     1681 tggctgatag gcaggaggcc gcacttcatc gccgacaccg cgacgcccgc gcgcgtgtgc
     1741 gtgtggatca cgcagttgat ctcgggccgc gccgcgtgca tgcagctgtg gatcacgaat
     1801 cccgcctcgt tcacgcccat gtcgagcgga tcgtcgagaa tcttgccttc ccagtcgatc
     1861 ttcaccaggt tcgacgcgct gatctcgtcg aacatcatcc cgtaggggtt gatgaggaaa
     1921 tcctcggtgc cgggaacacg cgccgaaaag tgggtgtaga tgaggtcggt ccacttgaag
     1981 attgcggcga gctggtaggc cgcagcgagg tcgacccgca cctgccactc ctccttgctc
     2041 actcgctcgc ggaccggggt gctcttgaac ttcgggttgg ctgacatgga tggatgataa
     2101 ggcgaaaccc ggaaatcggg gtcagagccc gatttcctac gccgcctggg cgatacggtc
     2161 ttcgaccacg tcgaaggcgc ggagcagcag ctccgggtca ttgcgcttga gctccacggg
     2221 gtcggacagc atccgtcgcc agagcttggc acgcggatgg ccatggtaaa gacccagcat
     2281 atggcgtgtc acgtggcgca gggaatcgac gttcttcgca tatccacaca tcactcttac
     2341 gacgtcgcgc cgcgtctttc cggggtcggc gagaaaccac ggattgctgt aagccgcccg
     2401 gccgatcatc acgccatcga cacgcctaag atgctcttga atctgttcct ttgacgcaat
     2461 tgcgccgttc acgacgatct cgaggccagg gaaatcgcgc ttcaaccggt agacgtagtc
     2521 gtacttcagc ggcgggatct cgcggttctc cttgggcgaa agccccttca gcaccgcgtt
     2581 gcgggcgtga acgataaacg tccggcagcc cgcccgcgcc accgtgccga cgaaatcgcg
     2641 tacgaacgag tagtcctcca ccttatcgat gccgacccga tgtttaacgg taaccggtat
     2701 ctgcacagcg tcacggatcg ccttcacaca cgtacccacg agctgcggct ccgccatcag
     2761 gcaggcgccg aaggcgccgc gctgcacccg ctcgctcggg cagccgatgt tcaagttgat
     2821 ctcgtcgtag ccgtactgct cgccgagctt cgcgcaatgc gcgagctccg cgggctcgct
     2881 gccgccaagc tggagggcaa caggatgctc ttcgctattg aactccaggt gccgcgggac
     2941 atcgccatgg aggagcgcgc cggtcgtgat catctcggta tgcaggcgcg cgcgcgaatt
     3001 gacctggcgc aaaaagaacc ggcaatggcg gtccgtcacg tccatcatcg gcgcgaccga
     3061 aatgccgtgg gacatccgct atttcatgtc cagcgcgcgg cgccgggtga gctcgatgaa
     3121 gcacgcgaga tggtggtcct cctgcgagac gcccgtgggc gtatggtcgc gccgccgcgc
     3181 gcattctgcg tcgcggtact ggcgccactc ttcgttcgcc ttgcggaagg tatcgaccgc
     3241 cttctcgttg cccgcgctgt ggtcgcgctc ccgcgcctcc tgaaacagga gcgcgtagct
     3301 ttgcatgagg tcgttctccg cgacctgcag cagcgtctgc aggcatgggc gcggttgctc
     3361 gcgcagcgcg gtatcgcgcg tgcattgcgc gagcgccgag gcgttggggc gcggcagcca
     3421 ttggaagaac acggcccgct tgtcgtcggc gagcaggcag atgaaggcga gctcggcgct
     3481 cggcgcgttg tcgagcacga ccgcgccgtt gcccctgagg atcgaggaca cgagctggtt
     3541 gccgagtttg cgcgtgtagc gctcgatgga cagcgcctgg tcgcgctcga tcacgatgtc
     3601 cttgccgccg gttccctcgc gcgccatttc gcgcttggcg taggcgcggc acgtttcgac
     3661 cgccggcccg ttgtattgcg cgttgacaga aaaggcggcc agcgctccca gcactcctat
     3721 cattagtctg gtcatcgata ttgccccgtg gattgcgcac gcgcgcggat cagcccatac
     3781 aggctctggg tgaacggcgc gcgcacgccg gccttgcgcg cgatctcgag cgtaccggcg
     3841 aggaggccgt cgatctcgag cggcttgccg gcctcgaggt cctg
//
LOCUS       DASTPD010000143         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_483746, whole genome shotgun sequence.
ACCESSION   DASTPD010000143 DASTPD010000000
VERSION     DASTPD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483746"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFR57_05035"
     CDS             <1..495
                     /locus_tag="VFR57_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0258771.1"
                     /translation="SVAFSTGGTTDVMAVTVLAKATGVEYNTVGFNSGGEALTALLGG
                     HVQASMGNPLEFMGQLQGGKVRALGVVRDQRFPALKDVPTFQEQGIKTPNFQMWRGIA
                     IPKEAPDAARVYWEGVMTKVNNSAAMKKYYADNVAQTASIPGKQFNEFLARQEKLYRE
                     LLGK"
     gene            500..931
                     /locus_tag="VFR57_05040"
     CDS             500..931
                     /locus_tag="VFR57_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0258772.1"
                     /translation="MRPGVNKGELVLALVFAALAALWIAKGATMPLWQGFAPDSGFLP
                     LLYGVLLFVLAGAVLVQLVAAKDARPGEAIRKPLVILGALVATVGALPIAGFAISVFA
                     LLLFLYAAVERLPMLPSAVVSGATTGVLYLIFKTWLGVPLP"
     gene            1065..2537
                     /locus_tag="VFR57_05045"
     CDS             1065..2537
                     /locus_tag="VFR57_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0258773.1"
                     /translation="MNELAQLASGFAAALTFKHLTMCFLGVLMGQMVGVLPGIGPSAA
                     IALLLPLTFGADPTSAIILFAGIYYGSQYGGTLTSVLVSVPGESSTVMTSIDGYQLAL
                     QGRAGPALGIAAIGSFIAGTLGTVGLMLLAPALAMAALAFGPPEYFALVVLGLTALAA
                     VGSSLLKGLVTGVAGLLLGTIGIDPQNGIARFDFGQSWLLDGVEFIVLTVALFGVGEV
                     VASSVLGVTRPVADVKSALPTRADWRRCRLPILRGTGVGFAIGVLPAAGATIASFIAY
                     IVEKKLAKDPSRFGKGAIEGVAAPEAANNAAAAGAMVPMLSLGVPGSGTTAIILGALI
                     MFGIRPGPELFAKNADLVWAVIASMYIGNVILLILNLPLAGLFARLLRVPYRWLYPPI
                     LALCIAGVYSQANSVEDCWLLAGLGALGWLMKRYDWPAAPMVLGLVLGPLFETALRQS
                     LTLSHGSGAIFLTRPISATLLALAFLAVTAPLLFKRKSAL"
     gene            2534..3346
                     /locus_tag="VFR57_05050"
     CDS             2534..3346
                     /locus_tag="VFR57_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0258774.1"
                     /translation="MKDRIGVDINQKLALEPAVGWAVEHKVPYIDLCLDQTPELLQKD
                     SPRLRKARARLEKAEIRLGLHTLSAVNIAETSPFVAEAADEYLGSYIDAAKRLGAGWI
                     VVHGGYHFGSDKRARMDAAVARIARAAKMAEKKKVKLLLENLNPEPDQAEVHYLANDL
                     EECRYYFERLDSPALGWSFTVNHAHIRPNRIEAFMAGLDMRRCGEVRLADCRGTHEEH
                     LRPGEGTIDFPAMFAAIEESGYRGHYMQAFGTLDDMLEGRALLAQLAKEGPK"
     gene            3343..4416
                     /locus_tag="VFR57_05055"
     CDS             3343..4416
                     /locus_tag="VFR57_05055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0258775.1"
                     /translation="MKRIGWLFALIACCGFAVEPDEIQRRKVPPLPSAQGDERGMANQ
                     IGPATYQRCAWHMQQPGAKAYELSHARSNTMPLSPFAGPYAVKPKATAGIPGTAHAFN
                     SEQFNENAEPAQQGTQMDALGHFGAFKQPWDGKSEFPADQAVYYGGFTQKDVKPTPDS
                     PLLKLGIEKAPPIVTSAVLFDAKAYKKKAMAAGELVTAQDLEGMLKVQGLGKRGVLPG
                     DVVYVRTGWSDHWRDPDTEKFYYTKAPGLSFDAAQWLAARRIVVIALDTPFVDTVAEG
                     QLQGKAGPAPGTPPGLPFAVHHHMLAQAGIHLIENAKLDELAAERVWTSCTMILPLRD
                     RGAAGSAVRPIAIGAPSKAGAQK"
BASE COUNT          721 a         1479 c         1465 g          755 t
ORIGIN      
        1 agcgtcgcgt tctcgaccgg cggcaccacc gacgtgatgg cggtcacggt gctcgccaag
       61 gcgaccggag tggaatacaa caccgtcggc ttcaacagcg gcggcgaggc gctcacggcg
      121 ctcctcggcg ggcatgtgca ggcctcgatg ggcaatccgc tcgagttcat gggccagctg
      181 cagggcggca aggtccgtgc gctcggcgtg gtacgcgacc agcgcttccc ggctttgaag
      241 gacgtgccca ccttccagga gcagggtatc aagacgccca acttccagat gtggcgcggc
      301 atcgccatcc cgaaggaggc accggatgcg gcacgcgtct actgggaagg agtgatgacc
      361 aaggtgaaca actccgcggc gatgaagaag tactacgcgg acaacgttgc ccagacggcg
      421 tcgatccccg gcaagcagtt caacgagttc ctggcgcgcc aggagaagct gtaccgggag
      481 ctgctcggca agtagataga tgcgtcctgg cgtcaacaag ggcgagctcg tgctcgccct
      541 tgtctttgca gcgctcgcag cgctgtggat cgccaagggc gcgaccatgc cgctctggca
      601 ggggttcgcg ccggactcgg gcttccttcc gctcctctac ggcgtgctgc ttttcgttct
      661 ggcaggcgcc gtgctcgtcc agcttgttgc ggcaaaggat gcacggccgg gtgaggcaat
      721 ccgcaagccg ctcgtcattc tgggcgcgct cgtcgccacg gtcggcgccc tgcccatcgc
      781 cgggttcgcg atctcggtgt tcgcgctcct gctctttctc tatgcagcgg tagagcggct
      841 gccgatgctt cccagcgcgg tagtgtcggg agcgaccacc ggagtgctgt atctgatctt
      901 caagacgtgg ctgggagttc cattgccatg atcagaattc ggttcgcccc cctgacaagg
      961 ggggaagcga gcgcctttcg cgagcggggg gttcgctttc caaagcccgc aaaccccccg
     1021 ttcgccttcg gctcacgacc cccttgtcag ggggtcaaag ggcgatgaac gagctcgcgc
     1081 agctcgcgag cggtttcgcc gccgcgctca ccttcaagca tctgacgatg tgcttcctcg
     1141 gggtgctgat ggggcagatg gtcggcgtgc tgccggggat cgggccgtcg gcggcgatcg
     1201 cgctgctcct gccgctcacc ttcggtgccg atcccacatc ggcgatcatc ctcttcgccg
     1261 gcatttatta cggctcgcag tacgggggca cgctcacctc ggtgctggtc agcgtgccgg
     1321 gggagtcgag caccgtgatg acgagcatcg acggctacca gctcgcgctc cagggccgcg
     1381 ccgggcccgc gctcggcatc gcggcgatcg gctctttcat cgctggcacg cttggcacgg
     1441 tcggtctgat gctgctcgcg cccgcgctcg cgatggcagc gctcgctttt ggaccgcccg
     1501 agtattttgc gctggtcgtg ctcggcctca ccgcgctcgc tgccgtggga tcttcgctgc
     1561 taaagggcct cgtcaccggg gtcgccgggc tccttctcgg cacgatcggc atcgatcccc
     1621 agaatggcat cgcgcgcttc gatttcggcc agagctggct gctcgacggc gtcgagttca
     1681 tcgtgctcac cgtcgcgctc ttcggcgttg gagaagtggt ggcgagctcg gtgctcggcg
     1741 tgacgcggcc ggtcgcggac gtgaagagcg cgctgcccac ccgcgccgac tggcgccgct
     1801 gccggcttcc gatcctgcgc ggcacgggtg tgggctttgc gatcggcgtg ctgcccgcgg
     1861 ccggcgcaac cattgcctcc ttcatcgcct acatcgtgga gaagaagcta gcgaaagatc
     1921 cgtcgcgctt cggcaagggc gcgatcgagg gggtcgccgc gcccgaggcg gcgaacaacg
     1981 ccgcggccgc gggcgccatg gtgccgatgc tctcgctcgg cgtgccgggc tcgggcacca
     2041 cggcgatcat tctcggcgcg ctcatcatgt tcggcatccg ccccgggccg gagctcttcg
     2101 ccaagaacgc cgacctcgta tgggcggtga tcgcgagcat gtacatcgga aacgtgatcc
     2161 tcctcatcct caacctgccg ctcgcgggcc tctttgcgcg gctcctcagg gtgccctacc
     2221 gctggctcta tccgcccatc ctcgcgctct gcatcgccgg ggtgtactcg caggcgaaca
     2281 gcgtcgagga ctgctggctg ctcgcaggac tcggcgcgct ggggtggctc atgaagcgct
     2341 acgactggcc ggcggcgccg atggtgctcg gcctcgtgct cggaccgctg ttcgagaccg
     2401 cgctgcgcca gtcgctcacg ctgtcgcacg gcagcggcgc catcttcctc acgcggccga
     2461 tctcggcaac gctgctcgcc ttggcgtttc tggcagtcac cgcgcctttg ctgttcaaaa
     2521 ggaaatccgc tttatgaaag acaggatcgg ggtcgacatc aatcagaagc tggcgctcga
     2581 gcccgcggtc gggtgggcgg tggagcacaa ggtaccttat atcgatctgt gcctcgacca
     2641 aacccccgag ctcctgcaga aggactctcc ccgtctaaga aaagcgaggg ccaggctcga
     2701 aaaggcggaa atccggcttg ggctccatac cctctccgcg gtgaacattg cggagacctc
     2761 gcccttcgtc gccgaggcgg ccgacgagta cctggggtcc tatatcgacg ccgcaaagcg
     2821 gctcggtgcc gggtggatcg tggtgcacgg cggctaccat ttcggctccg acaagcgcgc
     2881 acgcatggac gccgccgtgg cccgcatcgc gcgggcggcg aagatggccg aaaagaagaa
     2941 agtgaagctc ctgctcgaaa acctgaaccc ggagcccgac caggcggagg tccattacct
     3001 cgccaatgat ctcgaggaat gccgctacta cttcgagcgg ctcgactcgc cggcgctcgg
     3061 ctggagcttc accgtaaacc acgcccacat ccgcccgaac cgcatcgaag cgttcatggc
     3121 cggcctcgac atgcgccgct gcggcgaggt gcgcctcgcc gactgccgcg gcacgcacga
     3181 ggagcacttg cgcccaggcg aaggcacgat cgacttcccc gccatgttcg ccgccatcga
     3241 ggagagcgga tatcgcggcc actacatgca ggccttcggc accttggacg acatgctgga
     3301 aggacgcgcg ctgctcgcac agttggcaaa ggagggtccc aagtgaagcg catcggatgg
     3361 ctgtttgcct tgattgcttg ctgcggcttc gcggttgagc cggacgagat ccagcgacgg
     3421 aaggtgcctc cgctgccctc ggcgcagggc gacgagcgcg gcatggcgaa ccagatcggc
     3481 ccggccacct accagcgctg cgcctggcac atgcagcagc ccggcgcgaa ggcgtacgag
     3541 ctctcgcacg cgcgctcgaa cacgatgccg ctctcgccct tcgcggggcc ttacgccgtg
     3601 aagcccaagg cgaccgccgg cattcccggg accgcccacg cgttcaattc cgagcagttc
     3661 aacgagaatg cggaacccgc tcagcagggg acgcagatgg acgcgctcgg tcactttggc
     3721 gccttcaagc agccgtggga cgggaagtcc gaattccccg ccgaccaggc cgtctactac
     3781 ggtgggttca cccagaagga tgtcaagccg acgccggact cgccgctcct caagctcggc
     3841 atcgagaagg cgccgccgat cgtcacgagc gcggtgctgt tcgatgcaaa ggcctacaag
     3901 aagaaagcca tggcagcggg cgagctcgtg accgcgcagg acctcgaagg gatgctgaag
     3961 gtacaagggc ttggaaagcg cggcgtgctg cccggggacg tggtctacgt gcgtaccggg
     4021 tggagcgacc actggcgaga tcccgacacc gagaagttct actacaccaa ggcgccgggg
     4081 ctctccttcg acgcggcgca atggctcgcc gcgcggcgga tcgtcgtgat cgcgctcgac
     4141 acgcctttcg tcgacacggt ggcggaaggc cagctccagg gcaaggccgg gccggcgcca
     4201 ggcaccccgc ctggtctgcc cttcgcggtc caccaccaca tgctcgcgca ggccggaatc
     4261 cacctcatcg agaacgccaa gctcgacgag ctggcagccg agcgggtgtg gacttcgtgc
     4321 accatgatcc tgccgctgcg cgaccgcggg gcggcggggt cagccgtgcg cccgatcgct
     4381 attggtgcac cttctaaagc aggtgcacaa aaatgagaat
//
LOCUS       DASTPD010000144         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1505321, whole genome shotgun sequence.
ACCESSION   DASTPD010000144 DASTPD010000000
VERSION     DASTPD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505321"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..78
                     /locus_tag="VFR57_05060"
     tRNA            2..78
                     /locus_tag="VFR57_05060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Leu,seq:cag)
     gene            203..279
                     /locus_tag="VFR57_05065"
     tRNA            203..279
                     /locus_tag="VFR57_05065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Pro,seq:cgg)
     gene            286..364
                     /locus_tag="VFR57_05070"
     tRNA            286..364
                     /locus_tag="VFR57_05070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Arg,seq:acg)
     gene            374..450
                     /locus_tag="VFR57_05075"
     tRNA            374..450
                     /locus_tag="VFR57_05075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:408..410,aa:Lys,seq:ctt)
     gene            460..534
                     /locus_tag="VFR57_05080"
     tRNA            460..534
                     /locus_tag="VFR57_05080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:492..494,aa:Val,seq:tac)
     gene            624..699
                     /locus_tag="VFR57_05085"
     tRNA            624..699
                     /locus_tag="VFR57_05085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Asn,seq:gtt)
     gene            707..780
                     /locus_tag="VFR57_05090"
     tRNA            707..780
                     /locus_tag="VFR57_05090"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:739..741,aa:His,seq:gtg)
     gene            complement(807..1592)
                     /locus_tag="VFR57_05095"
     CDS             complement(807..1592)
                     /locus_tag="VFR57_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0258776.1"
                     /translation="MHGARAIRASFADGLSRDADLIGEDPDTDIAVVRIGAGGLVSVQ
                     LGSSRAVRVGQLAIAIGNPYGFQHTVTAGVVSALGRSLRSQTGRLIDDVLQTDAALNP
                     GNSGGPLVNSRGEAIGVNTAIIPGAQGLCFATAIDTVKWVVMQLLKDGRVRRGYLGIA
                     GANVPLARRIARHFDLHNTHAVRVESVEKDGPAYRAGLRPGDRIVRFDGAAVNGIDDL
                     HRALTGERIGAAAAIAFIRAPELVELEIRPAEAKRGGSKSRMR"
     gene            complement(1529..1834)
                     /locus_tag="VFR57_05100"
     CDS             complement(1529..1834)
                     /locus_tag="VFR57_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258777.1"
                     /translation="MDLDPRWILDESGAGPEPAGASGDESLLDAYSQAVVRATEAVAP
                     AVAHLEVELAGSKGDRKAAAQDSASRLTDFSSPTATWCTARAPSARASPTASRATPT"
     gene            complement(1904..2521)
                     /locus_tag="VFR57_05105"
     CDS             complement(1904..2521)
                     /locus_tag="VFR57_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0258778.1"
                     /translation="MLTLFYAPGACSMAAHIVLEESGEKYEAKRMDLAKGEQKTAEYM
                     KIHPLGRVPALKLDDGSPLSENTAILPFLGKRFGLWPADPVKEARALSVVGFFASTVH
                     PAHAHIGRPERYTEDKSAFPGLQAMGKKTFHEYLKQIDGMYAGREWLSDKYSVLDPYA
                     FVFYVWGVRRELPMAELKSYSAFRDRMMKRPAAQRVVADEGVKVS"
     gene            2654..3112
                     /locus_tag="VFR57_05110"
     CDS             2654..3112
                     /locus_tag="VFR57_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0258779.1"
                     /translation="MITLNVNGKRVQTDAEADTPLLWVLRDYLNMTGTKFGCGMALCG
                     ACTVHVDGQPTRSCTTPASAVVGKKVVTVERIGQTRVGAAVQAAWVAENVPQCGYCQS
                     GQVMAAAALLAAKPKPTDADIDEAMSGNLCRCATYPRIRAAIKRAAGSLA"
     gene            3109..>4179
                     /locus_tag="VFR57_05115"
     CDS             3109..>4179
                     /locus_tag="VFR57_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0258780.1"
                     /translation="MITNESRRDFLKASGALLLGVHLPVALAQSGPGKTVGAAAAKPF
                     EPNAFVRIGTDNTVTVVVKHLEMGQGTYTGLPTLVAEELDADWSQIRAVGAPTDAKQY
                     NNLFWGPAQGTGGSTAIPNSYEQMRKAGAAARAMLVAAAAEKWKAPASEIRVSRGVVS
                     WRDRRATFGQLAADAAKQPVPVDVQLKDPKDFVLIGKPARRTDARAKSNGTALFTQDV
                     KLPGMLTAVVLHPPRFGAKLKSFDAAKARAVKGVQEVVAFSTPVSDGVAVLAKDYWTA
                     KQGRDALTAEWDESNAFRLGSAEIMSQYEALAATPGLSARKDGDAEAALQNAARTLEA
                     SYEFPYLAHAALEPMNCVAQLSA"
BASE COUNT          721 a         1385 c         1386 g          687 t
ORIGIN      
        1 tgccccgctg gcggaatcgg catacgcgcg tgtctcagaa acacgcagat ccgggttcga
       61 atcccgggtg gggtaccaga attttttcgc cagagcagtc ggctggccgc tcttttacaa
      121 cttggatgtg gcaaagaaag gcccgccgcg cgtgcgtgcg aactgatcag gcgcgcggcg
      181 ggcggcggtt tgtcgccttc atctcgctgt agcgcagccg gccagcgcgc gtggtccggg
      241 accacgaggc cgcaggttcg aatcctgccg gcgagaccag tccatacctc cgtagctcag
      301 cggacatcag agtgcgcggt tacgacccgc gaggccgagg gttcgaatcc ttccggaggt
      361 gccatcccac cttcggcgcg tagctcaatt gcgcagagca gccggctctt aaccggcagg
      421 ctgcaggttc aagtcctgcc gcgccgacca attcgaaatg ctcggttagc tcagtcgaag
      481 agcgcctgtc ttacaaacag gaggccgatg gctcgatccc atcaccgagt accaactcgt
      541 gcttccctag ctggtctggt gacagcgccg ggctgaaaat ccggagagct ccgttcgaaa
      601 cggaggggaa gcgccagacg tcttgtccag tagctcaagc ggtggagcgg cgccctgtta
      661 aggcgagggc tgcaggttcg aagcctgcct ggacagccag tcgctggaga tcttggtgta
      721 acagcagcac ggcggaatgt gagtccgcga gtgccggtgc gagtccggca gatcccccca
      781 aagctttgcc acatccgggg tttacatcac cgcatcctcg atttgcttcc tccccgctta
      841 gcttcggcag ggcgtatctc gagctccaca agctccgggg cgcggatgaa cgcgatcgcg
      901 gcagccgcac cgatgcgctc gccggtgagc gcgcggtgca ggtcgtcgat gccgttcacc
      961 gcggcgccgt cgaagcgcac gatgcggtcg ccgggccgca gccccgcgcg gtaagcgggc
     1021 ccgtccttct cgaccgactc gacgcgcacc gcgtgcgtgt tgtggaggtc gaaatggcgc
     1081 gcgatgcgcc gcgcgagcgg cacgttggcg ccggcaatac cgaggtagcc gcgtcgcacg
     1141 cggccgtcct tgaggagctg catcaccacc catttcaccg tgtcgatcgc ggtggcgaag
     1201 cacagcccct gcgcgccggg gatgatggcg gtgttcacgc cgatcgcctc gccgcgagag
     1261 ttgacaagcg gcccgcccga atttcccggg ttgagcgccg cgtcggtctg cagcacatcg
     1321 tcgatcaggc gcccggtctg cgagcgcagg ctgcggccga gcgcgctcac cacgccggcg
     1381 gtgacggtgt gctgaaagcc gtaggggttg ccgatcgcga tcgcgagctg gcccacgcgc
     1441 accgcgcgtg acgagccgag ctggaccgac acaagtccgc ccgcgccgat gcgcaccact
     1501 gcaatgtcag tgtctggatc ttcgccgatc aggtcggcgt cgcgcgagag gccgtcggcg
     1561 aagctcgcgc ggatggcgcg cgcgccgtgc accacgtggc tgttggtgag gagaaatccg
     1621 tcaggcgtga agcagaatcc tgagccgctg ccttgcgatc gcctttactc cccgcgagct
     1681 cgacttcgag gtgcgccacc gccggcgcga ccgcttctgt ggcgcgcacg accgcttggg
     1741 agtaggcgtc gagcaggctc tcgtcgccgg atgccccggc cggctccggc cccgcgccgc
     1801 tctcgtccag tatccagcga ggatcgagat ccatcaccct caggtgggga cgaaagcggc
     1861 gagcgtcaag tcatgcacac cgcctcgacg ttggcggttg ccttcaactc accttgacgc
     1921 cctcgtcggc cacgacgcgc tgcgcggccg ggcgcttcat catccggtct ctgaaggcgc
     1981 tgtagctctt gagctcggcc atcggcagct cgcgccgcac gccccagacg tagaacacga
     2041 atgcataggg atcgagcacc gagtacttgt cggacagcca ctcgcgcccg gcgtacatgc
     2101 cgtcgatctg cttcaaatac tcgtggaaag tcttcttgcc catggcctgc aggccgggaa
     2161 aggccgactt gtcctcggtg tagcgctcgg ggcggccgat gtgcgcgtgc gcggggtgga
     2221 cggtggaggc gaaaaagccg acgacggaaa gcgcgcgcgc ctccttcacc ggatcggccg
     2281 gccacaggcc gaagcgcttg ccgaggaagg gcaggatcgc cgtgttttcg ctgagcggcg
     2341 agccgtcgtc gagcttcagc gcgggcacgc ggccgagcgg atggatcttc atgtactcgg
     2401 cggtcttttg ctcgcccttg gcgagatcca tgcgcttcgc ctcgtacttc tcgccgctct
     2461 cctcgagcac gatatgcgcc gccatcgagc aggcgccggg ggcatagaaa agcgtcagca
     2521 ttgcgggctc cttgtaaatt cgcgcgggtg ggagcggcga ttctagctcc ggctgccggc
     2581 cgcgctcgta ttacggttgt aacgatccgc aggtcgaatt gaggaggatg atgatgcaac
     2641 cgggagaagg gccatgatca cactgaacgt caacggcaag cgcgtgcaga ccgatgccga
     2701 ggccgacacg ccgctgctct gggtgctgcg cgattacctg aacatgaccg gcacgaagtt
     2761 cggctgcggc atggcgctct gcggcgcctg caccgtgcat gtggacggac agccgacgcg
     2821 aagctgcacc acgccggcct ccgccgtcgt tggaaagaaa gttgttactg tggaaagaat
     2881 tgggcaaacc cgcgtcggcg ccgctgtaca ggcggcatgg gtggccgaga acgtcccgca
     2941 gtgcggctac tgtcagtccg ggcaggtgat ggccgccgcc gcgcttctcg ctgccaagcc
     3001 gaagccgacc gacgcggaca tcgacgaggc gatgagcggc aacctctgcc gctgtgccac
     3061 ctacccgcgc atccgcgcgg ccatcaagcg cgccgccggg agcctcgcat gattaccaac
     3121 gaaagccgtc gcgacttcct caaggcctcg ggcgcgctgc tcctcggcgt gcatcttccc
     3181 gtagcgctcg cgcagtccgg ccccggcaag accgttggcg ccgctgccgc gaagcccttc
     3241 gaaccgaacg ccttcgtccg catcggtacc gacaacaccg tgaccgtggt ggtgaagcac
     3301 ctcgagatgg gccagggtac ttacaccggg cttccgacgc tggtggccga ggagctcgac
     3361 gccgactggt cgcaaatccg cgctgttggc gcgcccaccg acgccaagca gtacaacaat
     3421 ctcttctggg gcccggcgca gggcaccggc ggcagcaccg cgatcccgaa ctcctacgag
     3481 cagatgcgca aagcgggcgc cgccgctcgc gccatgctcg tcgccgccgc cgccgagaaa
     3541 tggaaagcgc cggcttcgga gatccgggtt tcaaggggag tggtgtcctg gagggaccgc
     3601 agggcaacat tcggtcaact cgcggcggac gcggcgaaac agcccgtccc cgtcgacgtc
     3661 cagctcaagg acccgaagga tttcgtcttg atcggcaagc ccgcgcgccg caccgacgcg
     3721 cgtgcgaagt cgaacggcac cgcgctcttt acccaggacg tgaagctgcc cggcatgctc
     3781 accgcggtcg tgctgcaccc gccgcgcttc ggcgccaagc tcaagagctt tgacgccgcg
     3841 aaggcaagag cggtgaaagg cgtgcaggaa gtggtcgcct tctccactcc ggtgagcgac
     3901 ggcgtcgcgg tgctcgcaaa agactactgg accgcgaagc agggccgcga tgcgctcact
     3961 gctgaatggg atgaaagcaa tgcttttcgt cttggttctg cagaaataat gtcccagtac
     4021 gaagcgctgg ccgccacgcc cggcctttcc gcgcgcaagg acggcgacgc ggaagctgcg
     4081 ttacagaatg ctgcaaggac gctcgaggca tcctacgagt tcccttatct cgcccacgcc
     4141 gcgctcgagc cgatgaactg cgtggcgcag ctctcggcc
//
LOCUS       DASTPD010000145         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_280032, whole genome shotgun sequence.
ACCESSION   DASTPD010000145 DASTPD010000000
VERSION     DASTPD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280032"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VFR57_05120"
     CDS             complement(<1..1535)
                     /locus_tag="VFR57_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0258781.1"
                     /translation="MDLIRVRGARTHNLKDLTLELPRNRLVVITGLSGSGKSSFAFDT
                     LYAEGQRRYVESLSAYARQFLELMAKPEVDLIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EVHDYLRLLFARAGTPYCPQHGLALEAQTVAEMVDQIAALPEETRIVILAPVIAGRKG
                     EQADLLGELRAQGFARVRIDGVVYELDATPRLAKNVKHSVDVVVDRLRVRADARQRLA
                     DSLETALRHADGKVVVMEAQSDREHLFSARYSCPACDYAVPELEPRLFSFNNPAGACP
                     RCDGLGMVEFFDPTRIVAHPNLSLAGGAIRGWDRRNHFYFSMLQSLARHYDFDIESPW
                     EMLEERIQDLILYGSGSESIAFRYPSERGRGTVKTHPFEGVIPNHERRYRETDSQVVR
                     EELAKLRNARACPECGGARLRREARHVRIGDTTLHEISAWPLKQALEFFRTLSLKGSR
                     AQVAERVVREIASRLEFLVNVGLEYLSLERSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLD"
     gene            1551..2963
                     /locus_tag="VFR57_05125"
     CDS             1551..2963
                     /locus_tag="VFR57_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258782.1"
                     /translation="MKLVTIARSTFPPPRLLMTERMSRAELRAGAGLAAVYGLRMLGL
                     FFILPIFAVHAVTLQGGHDLTLVGIAIGGYGLTQGLLQVPFGMASDRWGRKPIIYAGL
                     LVFAAGSFLGIVATDIWTAIAARTLQGAGAINSVAMALAADLTREQHRTKIMAMIGAT
                     IGITFALSLVGAPLLYRYIGMGGVFALTGLLAIVAIGVVKYWIPDQAPAADVVRGSSG
                     LRRAFEPELLRLNAGIFILHIVLYAMFVVVPPLFVQTGLQLPEHWKVYLPVILASFVL
                     MIPGVLYADRKNRPKPVLLASVALLVAVEAALAALDAGLAILAGLMLAFFVAFNVLES
                     ILPALISRAAPAQGRGAAIGVYNTTQTLGVFFGGLVGGWVASRYGTATVFAVCAVLSA
                     VWLLLAAGMRSPVQVNELSTITFSIASGVKLDGLHQALTGVHGVREAEVVATERIAHL
                     KVVPGQWDEHRVRKLITGEV"
     gene            2965..3429
                     /gene="ssb"
                     /locus_tag="VFR57_05130"
     CDS             2965..3429
                     /gene="ssb"
                     /locus_tag="VFR57_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0258783.1"
                     /translation="MASVNKVIIIGNLGADPETRYLPSGEAVTNIRVATTDRWKDKQS
                     GELKEATEWHRIAFFGRLATIAGEYLRKGSQVYVEGSLRTRKWQDKDGQDRYSTEIRA
                     DVMQMLGRREGGGEPRAESAPAAKAAGAQTAASSAAKKPAGKFDDLEDDIPF"
     gene            3492..>3733
                     /locus_tag="VFR57_05135"
     CDS             3492..>3733
                     /locus_tag="VFR57_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0258784.1"
                     /translation="MTETYALAALWIGLALAAALASIWLRVATSLSEILVGTLAGAVL
                     GVAALGVDQNWVKFLAGAGAILLTFLAGAEIDPDAFR"
BASE COUNT          598 a         1207 c         1247 g          681 t
ORIGIN      
        1 tcgtcgagga cgtacatcac ccccgtcagg cccgagccga tctggctcgc gagccggatg
       61 cgctgcgcct cgccgccgga aagcgtctcg gccgagcgct cgagcgagag gtattcgagg
      121 ccgacgttga ccaggaactc caggcggctc gcgatctcgc gcaccacgcg ctcggcgacc
      181 tgcgccctgg aacccttgag gctcagtgtg cggaagaatt ccagcgcttg cttcagcggc
      241 caggccgata tctcgtggag agtcgtgtcg ccgatgcgca cgtggcgcgc ctcgcggcgc
      301 agccgcgcgc cgccgcattc ggggcaggcg cgggcattgc gcagcttggc gagctcttcg
      361 cgcacgacct gagaatcggt ttcgcggtag cggcgctcat ggttaggaat tacgccttcg
      421 aacgggtgcg tctttaccgt gccacggccg cgctccgacg gatagcggaa agcgatgctt
      481 tcgctcccgg agccatacag gatgaggtcc tgaatgcgct cctccagcat ctcccatggg
      541 ctttcgatgt cgaagtcgta gtgccgggcg agcgactgca gcatcgagaa gtagaaatgg
      601 ttcctgcggt cccagccgcg gatcgcgccg ccggcgagcg acaggttcgg gtgggcgacg
      661 atgcgcgtcg ggtcgaagaa ctccaccata ccgaggccgt cgcagcgcgg gcacgcgccc
      721 gccgggttgt tgaacgagaa aagtcgcggc tcgagctcgg gcactgcgta atcgcacgcc
      781 ggacacgaat accgcgccga aaaaagatgc tcccggtcgc tctgcgcttc catcaccacg
      841 actttgccgt cggcgtgccg caacgccgtc tcgagcgaat cggcgagccg ctggcgcgcg
      901 tcagcgcgca cccgcagccg gtcgaccacc acgtcgaccg agtgcttgac gttctttgca
      961 aggcggggcg tcgcgtcgag ctcgtacacg acgccgtcga tgcgcacgcg ggcgaatccc
     1021 tgcgcgcgca gctcgccgag caggtcggcc tgctcgcctt tgcgcccggc aatcaccggg
     1081 gcgaggatca cgatgcgcgt ttcttcgggc agcgcggcga tctggtccac catttcggcg
     1141 acggtctgcg cctctagcgc gagcccgtgc tgcgggcagt aaggcgtgcc ggcgcgcgcg
     1201 aaaaggagcc tcaggtagtc gtggacctcg gtgaccgtgc cgacggtcga gcgcgggttg
     1261 tggctcgccg ccttctgctc gatggcgatc gcgggcgaga gcccttcgat caggtccacc
     1321 tcgggcttgg ccatcagctc gagaaactgc cgcgcgtagg cagacagcga ctcgacgtag
     1381 cggcgctgcc cttcggcgta gagcgtgtcg aacgcgaacg agctcttgcc cgagcctgaa
     1441 agcccggtga tgaccacgag ccggttgcgc ggcagctcga gggtcaggtc cttgagattg
     1501 tgggtgcgcg ctccgcgaac gcggatgaga tccatgctgc ggcttgaaaa gtgaaacttg
     1561 ttactatagc gcgctccact ttcccgccgc cacgcctcct catgaccgaa aggatgagcc
     1621 gcgccgagtt gcgcgccggc gccggcctcg cggccgtgta cggcctgcgc atgcttggcc
     1681 tgtttttcat ccttccgata ttcgcggtcc acgccgtcac tctgcagggc gggcatgacc
     1741 tcacgctcgt cggaatcgcc atcggtggat acggcttgac ccaaggcctg ctccaggttc
     1801 cgttcggaat ggcgtccgac cgctggggac gcaagccgat catctacgcg ggcctgctcg
     1861 tgtttgcggc cggaagcttt cttggcattg tggcgaccga catctggacc gccatcgcgg
     1921 cgcgcactct gcagggcgcc ggcgcgatca actcggtcgc catggcgctc gccgccgatc
     1981 tgacgcgcga gcagcaccgc accaagatca tggcgatgat cggcgcgacc atcggcatca
     2041 ccttcgcgct ctcgttggtc ggggcgccgc tcctatatcg ctacatcggc atggggggcg
     2101 tgtttgcgct gaccggcttg ctcgcaatag ttgccatcgg agtggtgaag tactggattc
     2161 ccgaccaggc gccggcggcg gacgtcgtgc gggggagctc cgggctccgg cgtgccttcg
     2221 agcccgagct gctgcgcttg aacgcgggga tattcatcct gcatatcgtg ctttacgcaa
     2281 tgttcgtggt ggtgccgccg cttttcgtgc agacgggatt gcagctgccg gagcactgga
     2341 aggtttactt gccggtcatt ctggcttcgt tcgtgctcat gattcccggc gttctgtacg
     2401 cggaccgcaa gaaccgtccg aaacctgtgc tgctcgcgtc agtcgcgctg ctggtcgcgg
     2461 tcgaggccgc gctcgccgcg ctcgacgctg gcctcgccat ccttgccggg ctgatgctcg
     2521 cttttttcgt cgcattcaac gtcctcgagt ccatcctgcc tgcgctgatt tcgcgcgccg
     2581 cgcccgctca gggtcggggc gcagcgatcg gcgtctataa caccacgcag acgctggggg
     2641 tattcttcgg cgggctcgtc ggtggctggg tcgcctcgcg ctacgggact gccacggtat
     2701 tcgcagtgtg cgcagtgctc tcggccgtgt ggctcctgct agccgccggc atgcgctcgc
     2761 cagtccaggt caacgagctt tcgactatta cgttttccat cgcatccgga gtgaagctcg
     2821 acgggctgca ccaagcgcta accggcgtgc acggcgtgcg cgaagccgag gtcgtggcta
     2881 cggaacgcat cgcgcacctg aaagtggtgc ctgggcagtg ggacgagcat agagtgcgta
     2941 aactgattac tggggaggtt tgacatggct tcagtgaaca aggtcatcat catcggtaac
     3001 ctcggcgctg atcccgagac gcgctatctg ccgagcggcg aggcggtgac gaacatccgc
     3061 gtcgccacca ccgatcgctg gaaggacaag cagagcgggg agctgaagga ggcgactgaa
     3121 tggcatcgca tcgctttctt cgggcggctc gccaccatag cgggggaata cctcaggaag
     3181 ggctcgcagg tgtacgtcga aggaagcctg cgcacccgca aatggcaaga caaggatggc
     3241 caggaccgct actcgaccga gatccgcgca gacgtgatgc agatgctcgg ccgccgcgaa
     3301 ggtggcggcg agccccgcgc cgaatcggca ccggctgcca aggcggccgg tgcgcagacg
     3361 gcggcgtcct cagcggccaa gaagcctgcg ggcaagttcg acgacctgga agacgatatt
     3421 ccgttctagg ttctccctta accgccgcag gctgatgatg cctacgtgaa gcatcgcacg
     3481 gaggcattgc aatgacggaa acctacgccc tcgcggcgct ttggatcggg ttggcgctcg
     3541 cagcggcgct cgcctcgatc tggctgcggg ttgccacctc gctctccgaa atccttgtag
     3601 gcacgctagc cggagccgtg cttggcgtgg cggcgctcgg cgtcgatcaa aactgggtca
     3661 agtttctcgc cggcgcaggc gcgatcttgc tcaccttcct cgccggcgcg gaaatcgacc
     3721 ccgatgcgtt ccg
//
LOCUS       DASTPD010000146         5738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_281218, whole genome shotgun sequence.
ACCESSION   DASTPD010000146 DASTPD010000000
VERSION     DASTPD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281218"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..376
                     /locus_tag="VFR57_05140"
     CDS             107..376
                     /locus_tag="VFR57_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258785.1"
                     /translation="MGKEECVYRRTEAGLEAWQRQDPALSAEHRHILGLLESDTHWDE
                     VRKLLRSHADHKRFADLVERGLVASVVAGPGSDLDFTNVFLRGAV"
     gene            complement(373..957)
                     /locus_tag="VFR57_05145"
     CDS             complement(373..957)
                     /locus_tag="VFR57_05145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258786.1"
                     /translation="MNRALIFLATTLLATTAAAQSGAPASEPYVGQPGKDVIWVPTPP
                     TLVEKMLDMAAVTAKDFVIDLGSGDGRNVIAAAKRGARALGVEYDQNLVQVSRRAAAA
                     EGVASRAKFMQGDMFEADISKATVLALFLLPDNLERLKPKFERLRPGTRIVTNGYQIA
                     GWDAKEVGVAGGDCAPWCTAYLYIVPAMPANRQD"
     gene            1059..2186
                     /locus_tag="VFR57_05150"
     CDS             1059..2186
                     /locus_tag="VFR57_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0258787.1"
                     /translation="MLRAAFAAAVSTIFLCFPAYAQAPKSVKVQSTWPASITLQDHLR
                     ILAERIDKLTQGSVKVETLAAGQIVPPFEVLDATNKKVLDGFHAIAYYWVGKNPAAAL
                     FAGPPGGPFGMDHMDFLGWLYVGGGLEMWRDFYQNDLKLNVTVWPAHPSSPQALGWFK
                     KPLKGVADFKGLKCRQTGLNSEVYAKLGQSVVNMAGGEIVPAAQRGVIDCAEWVGGVE
                     DLRLGLPGVFKYHYTPGMHENNSIGEFGLNLDVWKGFSPQQQEAINSAVKDTFITWIT
                     RWQKQNADAIEEMIKKHGVQIRRTPPDILLASLKAWDEVAREHSEKHPTFKKVYASQR
                     EYAAKVVPAKRYMFPPYSFAANYYWPQEGSAATAKKDAKQP"
     gene            2194..2439
                     /locus_tag="VFR57_05155"
     CDS             2194..2439
                     /locus_tag="VFR57_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258788.1"
                     /translation="MRWPTNLDRAGIERRVIEVREVVRKNGLLEIVGFFEGVEHMSAA
                     QVGANVVAALTWLQDKPQHRPLTTQLEMVAMNLKNLK"
     gene            complement(2441..2620)
                     /locus_tag="VFR57_05160"
     CDS             complement(2441..2620)
                     /locus_tag="VFR57_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258789.1"
                     /translation="MASEDKPNKVERAAKKAGQAVESTAHRAGKWAERTATRAGKWVE
                     RTADKTDKAMRRALE"
     gene            2819..3997
                     /locus_tag="VFR57_05165"
     CDS             2819..3997
                     /locus_tag="VFR57_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0258790.1"
                     /translation="MINRGDWEDAQWRSQACSLLGPGMRLRAGLKDIWSLFKQAVGAW
                     SDDYAPSMGAALSYYTLFSIAPLLLIVIAVAGWFFGDEAARGELTSGLHGLMGEEGAK
                     AVESMVANASKPKQGIIASVVGVFVLILGATTVFGELQNSLDRIWRAPAREKVSGLWR
                     ILRARLLSIGMILGIAFLLMVSLVLDALLHSLGRMWGTGAWEAVAQTVNTLAGFGLTT
                     AVFALIYKLIPRATIEWHDVWVGAAVTALLFTVGKFLIGLYIGRSAVASSFGAAGSLV
                     VVMIWVYYSAQIFLLGAEFTWVYSHAHGSRRGQKRPGAAEAQEAIAEEPSMPAPVRIA
                     PAPLPPLVASPDDLPVHRRKPLLSFGLAAAIGAIAGVLFRAKPDLAFHRSRPRLFSRW
                     "
     gene            4025..4654
                     /locus_tag="VFR57_05170"
     CDS             4025..4654
                     /locus_tag="VFR57_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258791.1"
                     /translation="MGGIKAVAAAVALAVAGGTGYWLYAEKQKSDQQRLVVALLGDTT
                     KQLRQALNGPPVADMVSKIDSNLKLAKAPRHPNLALAAEHYIHGAREIARRRTDAERF
                     AREAAMSRRALAMHMSAASSRDPYWIRVAYDLKRRVERDHFDLETSLKALQHLLRTLP
                     EAQKDLAAHVDASLLLEEAERRKAHERAEQEAKRAADELDKVRRLALPK"
     gene            4655..4840
                     /locus_tag="VFR57_05175"
     CDS             4655..4840
                     /locus_tag="VFR57_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258792.1"
                     /translation="MWRKWWLLFSVIWGGIAALQAAVILGTSEETEKALRPLLLGFAV
                     PAALYLLGWVWERLRRK"
     gene            complement(4866..>5738)
                     /locus_tag="VFR57_05180"
     CDS             complement(4866..>5738)
                     /locus_tag="VFR57_05180"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU0258793.1"
                     /translation="SGDIEAVARWKPQDATTNPSLLLASAEDPRYRHLMEPDVDRVFV
                     NFGCEILKHIPGRVSTEVDARLSFDTAKSIQKAERFISLYEKAGVSRDRVLIKLAATW
                     EGIKAAEVLERERIRCNMTLLFSFAQATACADAGVTLISPFVGRIYDWHRQQRKVEDI
                     DIAEDPGVASVTRIYNYYKKHGYKTQVMGASFRKPAQILALAGCDLLTIAPDLLEKLS
                     RAQGEVPRRLSAEMARAQSIDKLDLNEKIFRWQHNEDAMATEKLAEGIRRFDADARKL
                     EKLLTSLSAAVPAK"
BASE COUNT          955 a         1857 c         1886 g         1040 t
ORIGIN      
        1 gctcgagcgt tcctgacttt ccatgacgtg aaacattgtg ctatcgcgcg cgcgcactgc
       61 ggtttcgcgc ccatgatgac gcactaaagg ttgcgagaga gggacgatgg gcaaggaaga
      121 gtgcgtctac cgccgcaccg aggcggggct cgaggcctgg cagaggcagg accccgcgtt
      181 gtcggccgag catcggcaca tcctcggctt gctcgaaagc gatacccact gggacgaggt
      241 gcgcaagctt ctgcgcagcc acgccgatca caagcgtttc gctgacctcg tagagcgcgg
      301 actggtcgcg tccgtggtgg ccgggccagg ctcggacctc gacttcacca acgtcttcct
      361 ccgcggcgcg gtctagtcct gccggttggc cggcatcgcc ggcacgatat agaggtaagc
      421 ggtgcaccac ggggcgcaat cgccgcccgc gacgccgacc tccttggcgt cccagcccgc
      481 aatttggtag ccgttcgtca cgatccgagt tccgggacgc agccgctcga acttcggttt
      541 cagccgctcc aggttatcgg ggagaaggaa aagcgcgagc accgtcgctt tggaaatgtc
      601 ggcctcgaac atgtcgcctt gcatgaattt ggcgcggctc gcgacccctt cggcggccgc
      661 ggcacggcgt gagacctgga cgagattttg gtcatactcc acgccaagcg cgcgcgcgcc
      721 gcgctttgcg gcggcgatga cgttgcgacc gtcgcccgag ccgaggtcga tgacgaagtc
      781 cttggccgtc accgccgcca tgtcgagcat cttctcgacc agggtgggcg gcgtcggcac
      841 ccagatgacg tccttgccgg gctggccgac gtagggctcg ctggccgggg cgccggactg
      901 ggcggctgcg gtggtggcta gaagggtggt ggcaaggaag atgagtgcgc ggttcatagc
      961 caaggttaac gcgttagccg gctctgttgt tcccgccgcg gaatagtctt gccggagcgc
     1021 cggtgaacac cctagcatgc gctctcggga ggtgcgccat gcttagagct gcgttcgcag
     1081 ccgcagtttc gactattttc ctttgctttc ccgcctacgc gcaggcgccg aagtcggtca
     1141 aggtacagtc cacctggccg gccagcatta cgctccagga ccacttgcgc attctcgccg
     1201 agcgcatcga caagctcacg cagggctcgg tgaaggtcga gacgcttgcc gccgggcaga
     1261 tcgtgccgcc attcgaagtc ctcgacgcga ccaacaagaa ggtgctcgac ggattccacg
     1321 ctatcgccta ctactgggtc gggaagaacc ccgccgcagc gcttttcgcc gggccgccgg
     1381 gaggcccctt cggcatggat cacatggatt tcctcggctg gctgtacgtg ggcggcggcc
     1441 tcgagatgtg gcgcgacttc taccagaacg acctcaagct taacgtcacc gtctggccgg
     1501 cgcacccctc gagcccacag gcgctcggct ggttcaagaa gccgctcaag ggagtggccg
     1561 acttcaaggg cctgaaatgc cgccagaccg ggctcaactc cgaggtctac gccaagctcg
     1621 gccagagcgt ggtcaacatg gcgggcggcg agatcgtgcc tgcggcgcag cgcggcgtga
     1681 tcgactgcgc ggaatgggtg ggcggtgtcg aagacctgcg cctggggctc cccggcgtct
     1741 tcaagtacca ctacaccccc ggcatgcacg agaacaactc gattggcgag ttcgggctga
     1801 acctggatgt ctggaaggga ttttcgccgc agcagcagga ggcaatcaac tccgcggtga
     1861 aggacacctt catcacctgg atcacgcgct ggcagaagca gaacgccgac gcaatcgagg
     1921 agatgatcaa gaagcacggc gtgcagatcc gccgcacccc gcccgatatc ctgctcgcct
     1981 cgctcaaggc gtgggacgag gtggcgcgcg agcattcgga gaagcacccg accttcaaga
     2041 aagtgtacgc atcgcagcgc gagtacgcgg ccaaggtggt gccggcgaag cgctacatgt
     2101 tcccgccgta ctcgttcgcg gcgaactact actggccgca ggaagggtcc gcggccacgg
     2161 ctaagaagga tgccaagcag ccctgaggag actttgcgct ggcccacgaa cctcgaccgc
     2221 gccggcatcg agcgcagagt aatcgaggtg cgcgaggtgg tgcgcaagaa tgggctgctc
     2281 gagatcgtcg gcttctttga aggcgtcgag cacatgagcg cggcgcaggt cggcgccaac
     2341 gtggtcgctg cgctcacctg gttgcaggac aagcctcagc accggcccct taccacgcag
     2401 ctcgagatgg tggcgatgaa cctgaagaac ctgaagtagc ctactcgagc gcccggcgca
     2461 ttgccttgtc ggtcttgtcc gcggtgcgct cgacccattt gcccgcccgg gtggcggtcc
     2521 tctcggccca cttccccgcc cggtgcgccg tgctttccac agcttgtccg gccttcttgg
     2581 ccgcacgctc gaccttgttt ggcttgtcct ccgacgccac ggcggtggcc gcgaacgcaa
     2641 cagcaagtgc cagcgctcta gccgttatgc gacgccaaat tgcggaatca gcgactccca
     2701 ttaacaccat agggttacgc ctcccagtta cattctatgg gaggaaaacg acatgagtgc
     2761 ttgtgtctag caatcttaac gaggcccgcg agtactacgc cacacccggg ggcctgaaat
     2821 gatcaatcgg ggcgactggg aggatgccca gtggcgctcg caggcatgtt ccctgctcgg
     2881 gccgggcatg cgcctgaggg cagggttgaa ggacatctgg tcgctcttca agcaggccgt
     2941 cggcgcctgg tctgacgact acgcgcccag catgggcgcg gcgctctcct actacacgct
     3001 gttctcgatc gcgccgctct tgctcatcgt gatcgcggtc gccggctggt tcttcggcga
     3061 cgaggcggcg cgcggtgagc tcacttccgg gctgcacggc ctgatgggcg aggagggcgc
     3121 gaaagcggtc gagagtatgg tcgccaacgc gtcgaagccg aagcaaggaa tcatcgccag
     3181 cgtggtcggc gtctttgtgc tgatcctcgg cgcgaccacc gtgttcggcg agctgcagaa
     3241 ctcgctcgac cggatctggc gcgcgccggc gcgcgaaaaa gtgagcggac tgtggcgcat
     3301 cctgcgcgcg cggctcctgt cgatcggcat gatcctcggc atcgcgttcc tcctcatggt
     3361 gtcgctcgtg ctcgacgcgc tgctgcactc gctcggccgg atgtggggca ccggcgcttg
     3421 ggaggccgtc gcgcagacgg tcaacaccct ggccggcttc ggcctcacca ccgccgtgtt
     3481 cgcactcatc tacaagctga tcccgcgcgc gacgatcgag tggcacgatg tctgggtggg
     3541 tgcggcggtg actgcgctgc tcttcacggt cggcaagttc ctcatcgggc tctacatcgg
     3601 gcgaagcgcg gtcgcctctt cattcggcgc agccggctcg ctcgtggtgg tgatgatctg
     3661 ggtctactac tcggcgcaga tcttcctcct cggcgccgag ttcacctggg tctattcgca
     3721 cgcgcacggc tcgcgccgcg ggcagaaacg tcccggcgcg gcggaagcgc aggaggcgat
     3781 cgccgaagaa ccttccatgc cggcgcccgt gcgcattgct cccgcgccgc tgccgccgct
     3841 cgtcgcgtcg ccagacgatc ttccagtgca caggagaaaa ccgctcctct ccttcggcct
     3901 cgcggccgcg atcggcgcga tcgccggcgt gctgttccgc gcgaagcccg accttgcctt
     3961 ccatcgctcg cgtccgcgac ttttctcacg ctggtagttt gggatttggg tatcctcccc
     4021 gcccatggga ggcatcaagg ccgtcgcagc cgcggtcgcg ctcgccgtcg ccggtgggac
     4081 gggctattgg ctctacgcgg aaaagcagaa gagtgatcaa cagcgactcg tagtcgcgct
     4141 tctcggcgac accacgaagc agctgcgcca ggcgctgaac gggccgcccg tggccgatat
     4201 ggtgtcgaag atcgacagca acctcaagct cgccaaggcg ccgcggcacc cgaatttggc
     4261 gctcgccgcc gagcactaca tccatggcgc gcgcgagatc gcgcgccggc gcaccgacgc
     4321 cgaacgcttc gcgcgcgagg ctgcgatgag ccgccgcgcg ctcgcgatgc acatgtcggc
     4381 cgcgagctcg cgcgacccct actggattcg cgtggcgtac gacctcaaga ggcgcgtcga
     4441 gcgcgaccac ttcgacctcg agacctcgct caaggcgctg cagcacttgc tacgcaccct
     4501 tccggaagcg cagaaggatc tcgcggcgca cgtcgacgcc tcgcttcttc tcgaggaggc
     4561 ggagcggcgc aaggcgcacg agcgcgccga gcaggaagcg aagcgcgccg ccgacgagct
     4621 cgacaaagtc cgccgcctcg cgctgccgaa atagatgtgg cgtaagtggt ggctgctctt
     4681 ctcggtgatc tggggcggga tcgccgcgct ccaggccgcc gtcatcctgg gaacttcgga
     4741 agaaaccgag aaagcactac ggccgcttct gcttggcttc gcggtgcccg cggcgcttta
     4801 tctccttggc tgggtgtggg agcgcctgcg gcgcaagtaa ccgggtcctg actccgattc
     4861 ctcggttact tagcgggcac cgccgcgctg agcgaggtga ggagcttctc gagcttgcgc
     4921 gcgtctgcgt cgaagcgccg gattccctcg gcgagcttct cggtcgccat ggcgtcctcg
     4981 ttgtgctgcc agcggaagat cttttcgttc aggtcgagct tgtcgatcga ttgggctctt
     5041 gccatttcgg cagaaagtct tcttggaacc tctccctgcg ccctggacag cttttccagc
     5101 agatccggcg cgatggtgag gaggtcgcag cccgcaaggg cgaggatctg ggcgggcttg
     5161 cggaagctcg cgcccatcac ctgagtcttg tagccgtgct tcttgtagta gttgtagatc
     5221 cgcgttaccg acgcgacgcc gggatcctcg gcgatatcga tatcttccac tttgcgctgt
     5281 tgcctgtgcc agtcgtagat tcgaccgacg aagggcgaga taagcgtgac gcccgcatct
     5341 gcgcacgccg tggcctgggc aaacgagaag agaagcgtca tgttgcagcg gatgcgctcg
     5401 cgctcgagca cctcggccgc cttgatgccc tcccaggtgg ctgcgagctt gatcagcacg
     5461 cgatcgcggc ttaccccggc tttctcgtac agggagataa agcgctcggc cttctggatc
     5521 gatttcgcgg tgtcgaacga tagccgggcg tccacttcgg tggagacccg accggggata
     5581 tgcttgagga tctcgcagcc gaagttgacg aacacgcggt cgacgtcggg ttccatgagg
     5641 tgtcgataac gcgggtcttc ggccgaagcg agaaggagcg acggattcgt cgtcgcgtcc
     5701 tgcggcttcc agcgcgcgac ggcttcgatg tcgccgct
//
LOCUS       DASTPD010000147         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1098869, whole genome shotgun sequence.
ACCESSION   DASTPD010000147 DASTPD010000000
VERSION     DASTPD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098869"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /gene="pheT"
                     /locus_tag="VFR57_05185"
     CDS             complement(<1..1895)
                     /gene="pheT"
                     /locus_tag="VFR57_05185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU0258794.1"
                     /translation="MKVPESWLRSFCNPPLSGRELAEKLTMSGLEVETYEPVGAELSG
                     VVVGEVLAVEKHPNAEKLTVCKVKAGKETVQVVCGAPNVRAGMKAPLARIGTKEIRGV
                     QSQGMLCSARELKLSDDHAGLLELPSNLKSGSDVRAALGLDDHVMTLKLTPNRADCLS
                     VLGVAREVSAITGSKLKRVEIKPAPAKIKARHAVNLPTPDACGRFAGRVIRAVNARAE
                     TPSWMRQRLERSGQRSISALVDVTNYVMLELGRPLHVYDLDKLRGPIVVRWGRKGERV
                     LLLNEQEVEVDESVLCITDDSGVIGLGGIMGGESTKADAVTRNVFLESAFFYPDAIAG
                     RARRYGFTSDASHRFERGVDFRNNVDGIERATRLILEICGGEPGPTVDTVARLPRRKP
                     VNMRVSRAQKVIGAPVPAKDMAKVFQRLGFAFKASSGAFTVAPPSYRFDIGIEEDLIE
                     EVARLYGFERIAAQPPLAAAKMLPVPEARRSLHTLRERLAAADYREVINFSFVEPGWE
                     ADFAAESNPIRLLNPIVSQQSVMRTTLIGSLVANIRHNQARKVPRIRVFEVGRVFMRD
                     AGATDGPLSVGGLRQPVRIAAAAYGPMLPEQWDAPPRPVDFYDVKADLESMLAPLAAS
                     FEPAAHPA"
     gene            complement(1892..2893)
                     /gene="pheS"
                     /locus_tag="VFR57_05190"
     CDS             complement(1892..2893)
                     /gene="pheS"
                     /locus_tag="VFR57_05190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU0258795.1"
                     /translation="MEQLSVIVSRALADFAAAPDPAALENAKARYLGKAGELTAFQSS
                     LKSTAPEQRRELGARFNAAKQEIETALEARRRALADARLAAKLTEQALDVTLPGRGRG
                     RGGLHPVIRTWQRIEEIWRSIGFDVADGPEIETDWFNFTALNSPENHPSRSMQDTFYV
                     DLKDANSLPLVLRTHTSPMQVRYARMHKPPIKVIAPGRTYRVDSDATHSPMFHQVEGL
                     WIDTDISLADLKGVYAEFLRRFFENDKLEVRFRPSFFPFTEPSAEIDMKLKGKWLEIS
                     GSGQVHPAVVRNFGLDPERYIGFAFGSGLERLTMLRYGIEDLRLFYEGDLRFLEQFA"
     gene            complement(2908..3267)
                     /gene="rplT"
                     /locus_tag="VFR57_05195"
     CDS             complement(2908..3267)
                     /gene="rplT"
                     /locus_tag="VFR57_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU0258796.1"
                     /translation="MPRVKRGVTARARHKKVLQQAKGFRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRRRKREFRALWIARINAAVRELGMSYSVFMAGLKRANIEIDRKVLADLAVTD
                     KPAFDKIAAQVKATIAH"
     gene            complement(3277..3477)
                     /gene="rpmI"
                     /locus_tag="VFR57_05200"
     CDS             complement(3277..3477)
                     /gene="rpmI"
                     /locus_tag="VFR57_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0258797.1"
                     /translation="MPKMKSKRGAAKRFKVRPGGGIKRSQAYMRHILTKKSTKRKRHL
                     RGGVEVDASNKAQILSVLPYSR"
     gene            complement(3558..>3923)
                     /gene="infC"
                     /locus_tag="VFR57_05205"
     CDS             complement(3558..>3923)
                     /gene="infC"
                     /locus_tag="VFR57_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0258798.1"
                     /translation="LKQKQIQVKEIKFRPGTDEGDYKIKLGKLIQFLEEGDKAKVTLR
                     FRGREMAHKEFGERLLERVRKDLDTIGVVEQFPRLEGRQMVMVLAPKKKGPAPKAKAK
                     DEKEAKPAKTAAPPKRQAA"
BASE COUNT          623 a         1320 c         1242 g          738 t
ORIGIN      
        1 gccggatgcg ctgcaggctc aaacgaggct gcgagcgggg cgagcatgct ttccaggtcc
       61 gctttgacgt cgtagaagtc caccggccgc ggcggcgcat cccactgctc cggcaacatc
      121 gggccatacg ccgccgcagc aatccgcacc ggctgacgca gcccgccgac gctgagcggg
      181 ccgtccgtcg cgccggcatc gcgcatgaac acgcggccga cttcgaacac ccggattcgc
      241 ggcaccttgc gggcctggtt gtgcctgatg ttcgcaacga gcgagccgat cagcgttgtg
      301 cgcatcacgg actgctggct gacgatgggg ttgaggagcc ggataggatt cgattctgcg
      361 gcgaaatcgg cctcccagcc tggctccaca aagctgaagt ttatgacttc gcgataatca
      421 gccgcggcaa ggcgctcgcg cagcgtgtgc aaggatctcc gtgcctcggg aacgggcagc
      481 atcttcgcgg ccgccagcgg cggctgcgcg gcaatgcgct cgaagccgta aagacgcgcc
      541 acctcttcga tcaggtcctc ctcgatgcca atgtcgaagc ggtaggacgg aggggccacg
      601 gtgaaagcgc cggagctcgc cttgaacgca aagccaagcc gttggaacac cttcgccatg
      661 tccttcgccg gaaccggggc cccaatcacc ttctgcgcgc gcgacacccg catgttgacg
      721 ggcttgcgcc tgggcagccg cgccaccgtg tccacggtcg ggcctggctc gcctccgcaa
      781 atctcgagga tcagccgggt cgcgcgctcg atgccgtcga cattgttccg gaagtccacg
      841 ccgcgctcga agcggtgtga ggcgtcgctc gtaaagccgt agcggcgcgc gcgcccggcg
      901 atcgcgtcgg gatagaagaa cgccgactcg aggaacacat tgcgggtgac ggcgtcggcc
      961 ttggtgcttt cgccgcccat gatgccgccc agcccgatca cgccggagtc gtctgtgatg
     1021 cacagcacgg attcgtcgac ctcgacctcc tgctcgttga gaagcaggac cctctctccc
     1081 ttgcgacccc agcgcacgac gatcggcccg cgcagcttgt cgaggtcgta gacgtggagc
     1141 ggccgcccca gctccagcat cacgtagttg gtgacgtcga ccagcgccga gatcgagcgc
     1201 tgtcccgagc gctccaggcg ctggcgcatc cacgacgggg tttcagctcg agcattcact
     1261 gcgcggataa cacggccggc aaagcggccg caagcgtcgg gggtcgggag attcactgca
     1321 tgcctcgcct tgattttcgc gggcgccggc ttaatctcga cccgtttcag cttgctaccc
     1381 gtgatggccg acacctcgcg cgcgacgccg agaaccgaaa ggcagtcggc gcggttcggc
     1441 gtgagcttaa gcgtcatgac gtgatcgtcg agcccgagcg ccgcgcgaac gtcgcttcca
     1501 ctcttgaggt tcgaaggcaa ttccagcaga ccggcgtgat cgtccgagag cttcagctcg
     1561 cgcgccgagc acagcatccc ctggctttgc acgccgcgga tttctttggt gccgattctg
     1621 gcgagcggtg ccttcatacc ggcgcgcaca ttgggcgcgc cgcagaccac ctgcacggtc
     1681 tccttgcccg ccttcacctt gcagacggtc agcttctcgg cattcggatg cttctctacg
     1741 gcaagcacct cgcccaccac aaccccgctc aactccgctc cgacaggctc gtaggtctcg
     1801 acctcgagcc cggacatcgt gagcttttcg gcaagctcgc gtcccgacag cggagggttg
     1861 cagaagctgc gcagccagct ctcggggacc ttcacgcgaa ctgttccagg aagcggaggt
     1921 cgccctcgta gaacaggcgc aggtcctcga tgccgtaacg cagcatggta aggcgctcca
     1981 ggcccgagcc aaaggcgaag ccgatatagc gctccggatc gagcccgaag ttgcgcacca
     2041 ccgcaggatg cacctgcccc gagcccgaga tctcgagcca tttgcccttc agtttcatgt
     2101 cgatctcggc cgagggctct gtaaagggga agaacgacgg ccggaaacgc acctcgagct
     2161 tgtcattctc gaagaagcgg cgcaggaact cggcatagac gcctttcaag tcggcgaggc
     2221 tgatgtcggt atcgatccac agtccttcga cctgatgaaa catcggcgag tgcgtggcgt
     2281 cggagtcgac ccggtaggtg cgcccgggcg cgatgacctt gatcgggggc ttgtgcatgc
     2341 gcgcgtagcg cacctgcatc gggctggtgt gagtgcgcag tacgagcggc aggctgttcg
     2401 cgtccttcag gtccacgtag aacgtgtcct gcatagagcg cgacgggtga ttctcggggc
     2461 tgttgagcgc cgtgaaattg aaccagtcgg tctcgatctc ggggccgtcg gccacatcga
     2521 agccgatcga gcgccagatc tcctcgatgc gctgccaggt ccttatcacg gggtgcaagc
     2581 cgccgcggcc gcgcccgcgt ccgggcagcg tgacgtccag cgcctgctcg gtcagcttcg
     2641 cggcaagccg cgcgtcggca agggcgcggc ggcgcgcttc gagcgcggtc tcgatttctt
     2701 gctttgcggc gttgaaccgg gcgccgagct ccctgcgttg ctcgggagcg gtcgacttga
     2761 gcgaagactg gaacgccgtc agctccccgg ccttgccgag atagcgcgcc ttcgcattct
     2821 caagcgcggc cggatccggg gcggcggcga aatcggcgag cgcccgcgac acgatgacgc
     2881 tgagttgctc catctacggc ccgcaactca gtgagcgatc gtggctttca cctgcgccgc
     2941 gatcttgtcg aacgcgggct tgtcggtgac ggcgaggtcg gcgagtacct tgcggtcgat
     3001 ttcgatgttc gcgcgcttca gccctgccat gaacacgctg tagctcatcc cgagctcgcg
     3061 caccgcggca ttgatgcgcg cgatccacaa cgcgcggaac tcgcgcttcc tgcggcgccg
     3121 gtcgcggtag gcgtactgcc ccgccttcat caccgcctgc ttggcaaccc gatagacatt
     3181 cttcctgcgg ccgcggaagc ccttggcttg ctgcagtacc tttttgtggc gcgcgcgcgc
     3241 cgtgactcca cgcttgactc tcggcatggc gcgctcctat cgcgagtaag gcagaaccga
     3301 caggatctgc gccttgttcg atgcgtcgac ctcgacgccg ccgcgcaggt ggcgctttcg
     3361 cttggtgctc ttcttcgtga ggatatggcg catgtaggcc tgcgagcgct tgatgccgcc
     3421 gcccgggcgc accttgaagc gcttcgcggc gccccgcttg gatttcattt tgggcacgat
     3481 ctgccctcct cttctagtat gacgccgggc ggtccccgcc tatgcggcga cgcttcaaat
     3541 gccacggcca tcacttgtca agccgcttgg cgcttcggag gcgctgcggt ttttgccggc
     3601 tttgcttcct tctcgtcttt cgccttcgcc ttcggcgccg gtcccttctt cttgggggcg
     3661 agcaccatca ccatctgccg gccttcgagc ctgggaaact gctccaccac gccgatggtg
     3721 tccagatcct tgcgcacgcg ctcaagcagc cgctcgccga actccttgtg cgccatctcg
     3781 cggccccgga agcgcagcgt gaccttcgcc ttgtcgccct cctcgaggaa ctggatgagc
     3841 ttgccgagct tgatcttgta gtcgccctcg tccgtgccgg ggcggaattt aatctccttg
     3901 acctggatct gcttctgctt gag
//
LOCUS       DASTPD010000148         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_77639, whole genome shotgun sequence.
ACCESSION   DASTPD010000148 DASTPD010000000
VERSION     DASTPD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77639"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFR57_05210"
     CDS             <1..781
                     /locus_tag="VFR57_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEU0258799.1"
                     /translation="EDLKRRDVTINAMAKAEDGTLIDPFGGKKDLESGVLRHVSEAFA
                     EDPVRILRVARFAARFGFRLAEETLALMKHMVASGETDYLVPERVWQEFSRGLAEPEP
                     ERMFEVIQRTGFLAKAFPEIKAWPRRFSGSVPVRFAVLAWPLGEEQVLALCERLRTPG
                     DVRELALAAVRHKERLKESAADALLGVLKGADAFRRPERFAELLEAARLAEPGIDVAR
                     IERARAAAAAVDAGAIASSAQGADIGRLVDEARLEAIRELG"
     gene            complement(765..950)
                     /locus_tag="VFR57_05215"
     CDS             complement(765..950)
                     /locus_tag="VFR57_05215"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEU0258800.1"
                     /translation="MLKWLVTMLVVLVIFAVLAPRFGAWRLPGDFSIPVRGRIYYIPL
                     ASTLLFSLLVWLISRVL"
     gene            949..1566
                     /locus_tag="VFR57_05220"
     CDS             949..1566
                     /locus_tag="VFR57_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0258801.1"
                     /translation="MIELYTWSTPNGRKVSIMLEECGLRYKVHKINIGTNSEQFTPEY
                     LKINPNGKIPSIVDPDGPDGKPIAMMESGAILIYLADKTGKFFPQESKYQVLQWLMFQ
                     MGGVGPMFGQAHHFMRAKKDEIPYGSERYGNEAKRLYGVMDKRLSERDCFAGEYSIAD
                     IAIYPWVARHEWHRVDLTAFPNVKRWYDRVGARPAVAKGMAVPQV"
     gene            complement(1496..2947)
                     /locus_tag="VFR57_05225"
     CDS             complement(1496..2947)
                     /locus_tag="VFR57_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0258802.1"
                     /translation="MEALIGLAMLVVMMGAIFVGFPVAFTLLVLAFGFGALVLEGRVF
                     DLAFFQTTGLMRDELLAAVPLFIFMGYLVEKAGLMERLFLALRLALAPLRGSLFLVVI
                     LTATVFAMATGIVGAAVPVLGLMAAPIMLRAGYSAQLAAGAITAGGTLGILIPPSVML
                     LVLGPVLGVSVVDLYAAAMLPGLLLAALYLGYVLVRCHLDPSLGPPLPPSLRAASRLG
                     IAREMLIGVIPLAVLIAATLGSILAGLATPTEAAGVGTLGALALALAYGRLDWALLKG
                     VSLRTLATSSMVLFLAVASNVFGAVFARLGTAAWITDWLLGLTVPPILMLIVIVALLF
                     VLGWPFEWPAIVLVFVPIFYPVVLALGLDMIWFGALVAVTLQTAFLSPPVAMSGYYLK
                     QVAPAWPLATIFKGMLEFMAIQAVAIGLLLAFPSIATWLPEQLHRQPPALDIFEVEPY
                     PDERGIYEARITPAAPPCLSPPPAVRPPCHTSA"
     gene            complement(2935..3429)
                     /locus_tag="VFR57_05230"
     CDS             complement(2935..3429)
                     /locus_tag="VFR57_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0258803.1"
                     /translation="MLRLASAIDRFTEKSALVFASLIAPLLAAVTWEVVARYAFDAPT
                     SWAYETIYALYAAMFMLGAAYALRTGAHIRTDFLWEKWPVRTRAAIDSIAYLACFFPA
                     MLLFLVAGLEATWNSYLIGERSADTAWFPPLWPLKALVPAAALLLLLQGLSELLKSLH
                     AWKR"
     gene            complement(3429..4079)
                     /locus_tag="VFR57_05235"
     CDS             complement(3429..4079)
                     /locus_tag="VFR57_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258804.1"
                     /translation="MRVLFAAALFLTFPALADGISEAKKRWEASPHGPLLERILPPTF
                     DAARLPQRQSNGAKLVVAYCVQCHNLPNPAMHHAEKWPSVVQRMVLRMEGKGNMGTLM
                     AEMMAGVKAPAAEDTKAIVAYLQRHAQQPLDPARYPEVNQPSGEAFRLACNQCHVLPD
                     PQRYTAAEWPRVVARMRENMEWMNRVVGSRPVPGEPQLAVEEINAFLARYARKPGG"
     gene            complement(4083..5114)
                     /locus_tag="VFR57_05240"
     CDS             complement(4083..5114)
                     /locus_tag="VFR57_05240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0258805.1"
                     /translation="MLEELRAEIAAGGGWMPFARYMELAVGVYYGRGDRQFGAKGDFV
                     TASELGKLFARTLARQLRELDGPILEFGAGSGALAEVLTDELDCEYLILETSDALRAR
                     QAARLAGKVGFLDRLPDAIRGVVIANEVVDAMPVHAVAWKQSGIFERGVAFPGKLVWD
                     ERPAHGAVLEAAQEIEVPVPYESEIGLAARAWMDTLAERLESGAIFVIDYGFPSREYY
                     HPQRSMGTLMCHARHRAHEDPFERPGEQDITAHVDFGALARAAHAGGLELLGYATQAQ
                     FLVNCGITEVLEQENLKKALHYAPIAAEAQVLLSPNEMGELFKVLAVGRGVKQPLLGF
                     SCGDRSNRL"
     gene            5146..>5541
                     /locus_tag="VFR57_05245"
     CDS             5146..>5541
                     /locus_tag="VFR57_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258806.1"
                     /translation="MDDSLAGKAALVTGGARRVGAAIARRLHAAGAAVLIHYRDSEAD
                     AARLASALNAARPKSAVKVKAELLAPVAPRALVGAALETFGRLDLVVNNASSFFPIAV
                     GAMEPSHWEELIGSNLRAPLFIAQQAAAEL"
BASE COUNT         1048 a         1814 c         1853 g          826 t
ORIGIN      
        1 ggaagacctc aaacgccggg atgtcaccat caacgcgatg gcgaaggccg aggatgggac
       61 tctgatcgat cccttcggcg gcaagaagga tctcgagtcg ggcgtattgc gccacgtgag
      121 cgaagcattt gcggaggacc ctgtccgcat cctccgcgtc gcgcgttttg cggcgcgctt
      181 cggttttcgc ctggccgaag aaaccctggc acttatgaag cacatggttg cttccggcga
      241 aaccgactac ttggtccccg agcgcgtgtg gcaggagttc tcgcgcggcc tggctgaacc
      301 cgaacccgag cggatgttcg aggttatcca gcgcacgggg ttccttgcca aggcgtttcc
      361 cgagatcaag gcatggccgc gccgattctc ggggtcggtg cctgtgcgct tcgccgtgct
      421 cgcatggccg ctcggagaag agcaggtgct ggccttgtgc gagcggttgc gcacgccggg
      481 cgatgtacgc gagctcgcgc ttgccgctgt gcgccacaag gaaagactga aggaatccgc
      541 ggcagacgca ttgctcggcg tattgaaggg cgccgatgcg ttccgccgcc cggagcggtt
      601 cgcggagctc ctcgaagcgg cgcggctcgc cgagccggga atcgacgtgg cacgcatcga
      661 gcgcgcgcgc gctgccgcgg cggcggtcga cgcgggcgcg atcgccagct cggcgcaggg
      721 tgcggacatc ggccggctcg tcgacgaggc gcggctcgag gcgatcagag aactcggctg
      781 atcagccaga ccagaagcga aaacagcagc gtgctcgcga gaggaatgta gtagattcgt
      841 ccgcgcaccg ggatgctgaa gtcgcccggc aggcgccagg cgccgaagcg cggcgcgagc
      901 accgcgaaaa tcaccaagac cacgagcatg gtcacgagcc acttcaacat gattgaactc
      961 tatacctgga gtacaccgaa cggccgcaaa gtttccatca tgctcgagga atgcgggctg
     1021 cggtacaagg tgcacaagat caacatcggc acgaactccg agcagttcac gccggagtac
     1081 ttgaagatca atcccaacgg caagatcccg tccatcgtcg accccgacgg cccggacggc
     1141 aagccgatcg cgatgatgga atcgggcgcg atcctcatct acctggcgga caagacgggg
     1201 aagttttttc cgcaagagtc caagtaccag gtcctgcagt ggctcatgtt ccagatgggc
     1261 ggggtggggc cgatgttcgg ccaggcgcac catttcatgc gcgccaagaa ggacgagata
     1321 ccgtacggca gcgagcgcta cggcaacgag gcgaagcggc tgtatggggt catggacaag
     1381 aggctttcgg aaagggattg ctttgccggc gaatattcca tcgccgacat cgcgatctac
     1441 ccgtgggtcg cgcgccacga atggcaccgc gtggatctga cggcatttcc caacgtcaag
     1501 cgctggtatg acagggtggg cgcacggccg gcggtggcga aaggcatggc ggtgccgcag
     1561 gtgtgatgcg cgcctcgtag atgccgcgct cgtcgggata aggctcgacc tcgaagatat
     1621 cgagcgcggg cggctgccgg tggagctgct cgggaagcca ggtcgcaatg gagggaaacg
     1681 cgagcagcag gccgatggcc accgcctgga tcgccatgaa ttcgagcatc cccttgaaaa
     1741 tcgtcgcgag cggccaggcg ggtgccacct gcttgaggta gtagcccgac atcgccaccg
     1801 gtggcgaaag gaacgcagtt tggagcgtga cggcgaccag cgcgccgaac cagatcatgt
     1861 cgagcccgag cgccaagacc acgggataga aaatgggcac gaacaccagc acgatcgccg
     1921 gccactcgaa tggccagccg agcacaaaga gcagcgccac gatgacgatc agcatcagta
     1981 tcggcggcac cgtgagaccg agcagccagt cggtgatcca ggccgctgtg ccgagccggg
     2041 cgaacacggc gccgaacacg ttcgacgcaa ctgcgagaaa gagcaccatg ctcgaagtcg
     2101 cgagcgtgcg cagcgacacg cctttcagta gcgcccagtc gagccgtccg taggcgagcg
     2161 caagcgccag tgcgccgagc gtgccgaccc ctgctgcctc cgttggcgtg gcgaggccgg
     2221 caaggatgga gccgagcgtc gccgcaatca gcaccgcgag cgggatcacg ccaatcagca
     2281 tctcgcgcgc gatgccgagg cgcgaggcgg cgcgcagcga cggcggcagc ggcggcccga
     2341 ggctcggatc gaggtggcag cgtacgagga catacccgag gtagagggcc gcgagcagca
     2401 gcccgggcag catcgcggct gcgtacaggt cgacgaccga aacgccgagc accggcccga
     2461 gcacgagcag catcacagac ggcgggatca gaattcccag cgttccaccg gccgtgatcg
     2521 cccccgccgc gagctgcgcg ctgtagccgg cgcgcagcat gatgggcgcc gccatcagcc
     2581 cgagcaccgg tacggcggcg ccgacgattc cggtcgccat cgcgaacacc gtggcggtga
     2641 ggattacgac caggaaaagc gacccgcgca gcggcgcgag cgcgagacgc agcgcgagga
     2701 aaagccgctc catcaggccc gccttctcga cgagatagcc catgaagatg aagagcggga
     2761 ccgcggcaag cagctcgtcg cgcatgaggc cggtcgtctg gaagaatgcg aggtcgaaca
     2821 cccggccctc gagcaccagc gcgccgaagc cgaacgcgag cacgaggagc gtgaaggcga
     2881 ccgggaagcc aacaaagatc gcgcccatca tcacgaccag catcgcaagg ccgatcagcg
     2941 cttccatgcg tgcaggctct ttagcagctc agacagtcct tgcagcagca gaaggagcgc
     3001 cgctgccggg accaaggcct tgagaggcca aagcggggga aaccaggccg tgtcggccga
     3061 gcgctcgccg atcaggtacg aattccacgt cgcctcgagg cccgcaacga gaaagagcag
     3121 catggctggg aagaagcacg cgaggtaggc gatgctgtcg atcgcggcgc gcgtgcgcac
     3181 aggccatttc tcccacagga agtcggtacg gatatgcgcc ccggtgcgca aggcgtacgc
     3241 ggcgccaagc atgaacatcg ccgcgtagag ggcgtagatg gtctcgtaag cccacgaggt
     3301 aggcgcgtcg aacgcgtagc gcgcgacgac ttcccacgtc acggcggcga ggagcggcgc
     3361 gatgagcgat gcgaatacca gcgcgctctt ctcggtgaag cggtcgatcg cgctggccag
     3421 gcgcagcatc agccgcccgg cttgcgcgcg tatctcgcca ggaaggcgtt aatctcctcg
     3481 accgcgagct gcggttcgcc gggcaccggc ctggagccga ccacgcggtt catccactcc
     3541 atgttctccc gcatccgcgc cacgacgcgc ggccactcag cggcagtgta gcgttgcgga
     3601 tcgggaagca cgtggcactg gttgcatgcc aggcggaacg cttcgccgga gggctggttc
     3661 acctcggggt aacgcgccgg gtcgagcggc tgctgcgcgt ggcgctgcag gtaggcgacg
     3721 atggccttgg tgtcctccgc ggcgggcgct ttcactccgg ccatcatctc cgccatcagc
     3781 gtgcccatgt tgcccttacc ctccatgcga agcaccatgc gctgcacgac gctcggccac
     3841 ttctctgcat ggtgcatcgc cggattgggg agattgtggc actgcacgca ataggccacc
     3901 accagcttcg ctccgttcga ctgccgctgc ggcagccgtg ccgcatcgaa ggtcggcggg
     3961 agaatgcgct cgagcagggg cccgtgcggg ctcgcctccc agcgcttttt cgcctccgaa
     4021 atcccatcgg cgagcgccgg gaacgtcagg aataaagccg ccgcaaacag cacgcgcatg
     4081 ccctaaagcc tattggaacg atccccgcat gaaaacccca ggagcggctg tttgaccccg
     4141 cgcccgaccg cgagcacctt gaaaagctcg cccatctcat taggcgagag caggacttgg
     4201 gcctcggccg cgattggcgc gtagtgcagg gctttcttca ggttctcctg ctcgagcact
     4261 tcggtgatgc cgcagttcac caggaactgt gcctgcgttg cgtaaccgag caactcgagg
     4321 ccgccggcat gcgcggcgcg tgcaagggcg ccaaagtcca cgtgcgcagt gatgtcctgc
     4381 tcgcccggac gctcgaacgg gtcttcgtgc gcgcggtggc gcgcgtggca catcagcgtt
     4441 cccatcgaac gctgagggtg gtagtactcc cgcgaaggaa agccgtagtc gatgacaaag
     4501 atcgcacccg attcgagccg ttcagcaagt gtgtccatcc aggcccgtgc ggccagcccg
     4561 atctcgcttt cgtaaggaac cggcacttct atttcctgcg ctgcttcgag caccgccccg
     4621 tgcgccggcc gctcgtccca cactagcttt ccggggaagg cgactccgcg ctcgaagatt
     4681 ccgctctgct tccacgccac cgcgtgcacc ggcatggcgt ccacgacttc gttcgcgatg
     4741 accacgcccc ggatggcgtc gggaagacga tctaggaagc ccaccttgcc ggcgagcctc
     4801 gccgcctggc gcgcgcgcag cgcgtcgctc gtctcgagga tcaggtattc gcagtcgagc
     4861 tcgtccgtga gcacctcggc caacgcaccg ctgcctgccc cgaattccag gatcggtccg
     4921 tcgagctccc gcagctgccg ggcgagcgtg cgggcaaata gttttcccag ttcggacgcg
     4981 gtcacaaaat cgccctttgc gccgaactgc cggtcgccgc ggccgtaata gacccccact
     5041 gcgagctcca tgtagcgggc gaaaggcatc cagccgccgc cggcggcgat ctctgcgcgc
     5101 agctcctcaa gcacccggga aatctaacct agaatcagcg gcttcatgga tgattccctg
     5161 gcaggcaagg cggcgctcgt caccggcggc gcacgccgcg tcggcgcggc gatcgcgcgg
     5221 cggctgcacg cggccggcgc agccgtactg atccactacc gcgactccga ggccgatgcc
     5281 gcgcgcctcg cctccgcgct gaatgcggcg cgcccgaaga gcgcggtaaa ggtgaaagcc
     5341 gaactcctcg cgcccgtggc gccgcgcgcg ctggtcggcg cggcactcga gactttcggg
     5401 cgcctcgatt tggtcgtgaa caacgcctcg agcttcttcc cgattgccgt aggcgccatg
     5461 gagccttcgc actgggagga gcttatcggc tcgaacctgc gcgcgccgct cttcatcgcg
     5521 cagcaggcgg ccgccgagct c
//
LOCUS       DASTPD010000149         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_283732, whole genome shotgun sequence.
ACCESSION   DASTPD010000149 DASTPD010000000
VERSION     DASTPD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283732"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFR57_05250"
     CDS             <1..1255
                     /locus_tag="VFR57_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEU0258807.1"
                     /translation="LALREVSARMRPETALLRSLSHPGALSLVEQNFDFDLLTPAKLL
                     EKYVGRNVKIVRTHPTTGVESFETATVLAASSGVVLRIGDRIETGLPGRIVYDGVPPN
                     LRDRPTLVTELDSARGGMQNVELSYLSSGLAWKADYVAELNGADTALDLNGWVTLTNT
                     SGTAYPNAKLQLVAGDINRVREELRLSQRAMADRVAEAAPASKMAQEALFEYHLYTLA
                     RPTTIADNQTKQVALLSAQAVPVVKELVLQGSDYYYRSSVGGIGQKMKVGVFVQLENR
                     ESSHLGMPMPKGVVRVYKKDGAGNAQFVGEDRIDHTPKNETVRLKLGEAFDLTADKKQ
                     TDFKRREPTNRASYVFESAYEIVLRNAKKEAASVVVREPVPGDWTMLEETQRHAKVAA
                     GTAEWRVHVPAEGSTTLRYRVLVRY"
     gene            complement(1252..1863)
                     /locus_tag="VFR57_05255"
     CDS             complement(1252..1863)
                     /locus_tag="VFR57_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEU0258808.1"
                     /translation="MSFHVKELVIAGWTGRDEAALRKHIKELEEIGVKPPKTTPIFYR
                     VAANLFCHAEEIQVSGPDTSGEVEFVLIRDDDLRVAVGSDHTDRKAETIGVSLSKQLC
                     AKPVSRDSWRYEEVKPHWERLVLRSWADSELYQEGPVSAMRSPEDLMSRYPLKPQWAM
                     FGGTLAAKGGIRPAVRFTMQLEDPVLGRKLRHEYRITVLPVEG"
     gene            complement(1860..3326)
                     /locus_tag="VFR57_05260"
     CDS             complement(1860..3326)
                     /locus_tag="VFR57_05260"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0258809.1"
                     /translation="MLKSGNEHLESLRDGRVVYIGSERVADVTTHPAFRNAARTVASI
                     YDMKLAERDAMSFEQDGERFSIYFLQPKTREDLARRTRGHRMIADLTYGMFGRSPDHV
                     ASFVTGMAMKPEELKAPCGRPENLLNFYRDIARTDAYVVYAVVPPQAARNPEFYQRQN
                     IPIPTLSVVREEDDGLVISGMKMLATGAVFANEVWIGNVLPLAADQKKQAITCSLPIA
                     TKGLSLWSRKPFEREATSEFDAPLAWRFDETDSMVLCDEVKVPWERVFVHDDALLSRD
                     IYVKTPSHCFGNHQSNVRYWSKMRLLLGVCSKIAHATGAIEVPAVKEVLGHMAAVESM
                     IGGMVNGQIEALERWPDGYVCFNRRMMYAALEWCTQNYSDFIERLRELCGGGVFQMPA
                     DISVVENEELALKFTTYFQTPQSGAIERMKLFKLAWDMVGSEFAGRHLQYEKFYAGAS
                     FIVRNHAFRETPWNEFHKIVDDLMGTYDIPRSQAKRVA"
     gene            complement(3307..3693)
                     /locus_tag="VFR57_05265"
     CDS             complement(3307..3693)
                     /locus_tag="VFR57_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEU0258810.1"
                     /translation="MMKPKQHLEFVRVDLNSGWEVPPGYPSGFYQKILASDLDEKKKT
                     GSRTRLLKIEPGAFSTEPFVHDHWEEVYLVQGDLVVGNDKQGKGGTQCFAPTYACRPP
                     GVYHGPFTSRAGCILHEIHYYAQERK"
     gene            complement(3785..4321)
                     /locus_tag="VFR57_05270"
     CDS             complement(3785..4321)
                     /locus_tag="VFR57_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0258811.1"
                     /translation="MARPRKKTPPLTVSRAALLTRGSDDEFRRLIHDLIAYGHKLDAC
                     RDAFAAIAGISGAQYEILMLVSRADGLSVGEVAARMHRSGAFITIEANKLVAAGILEK
                     AADPADGRRVLLRMNEKSVQLLEHMAPYQRRVNDVLFEPLDAKRFRELRTLGAELVAC
                     GDRAVAMLELLLHEAEAA"
     gene            4371..>4597
                     /locus_tag="VFR57_05275"
     CDS             4371..>4597
                     /locus_tag="VFR57_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258812.1"
                     /translation="MAKTGDLREALAALERGDWQAAHAIVQADEDSRLACWAHGIVHL
                     MEGDTANARYWYRRAGRPFRQDVAAEIAALDA"
BASE COUNT          799 a         1520 c         1486 g          792 t
ORIGIN      
        1 cctggcgctg cgcgaggtga gtgcccggat gcgtccggag accgcgctgc tgcgcagcct
       61 gtcgcacccc ggagcgctca gcctcgtaga gcagaacttc gacttcgatc tcctcacgcc
      121 agccaagctg ctcgagaagt acgtcggccg caacgtgaag atcgttcgca ctcatccgac
      181 gaccggggtc gaatccttcg agaccgccac cgtgctcgcg gcgagctccg gcgtcgtcct
      241 gcgcatcggc gatcgcatcg agaccggact gccgggacgc atcgtctacg acggcgtgcc
      301 gcccaacctg cgcgaccggc cgacgctcgt gaccgaactc gacagcgcgc gcggcggcat
      361 gcagaacgtc gagctctcct atctctcgag cggcctcgcc tggaaggccg actacgtggc
      421 cgagctgaac ggcgccgaca ccgcgctcga cctgaacggc tgggtgacgc tcaccaacac
      481 gagcgggacc gcgtacccca acgcgaagtt gcagctcgtc gccggcgata tcaatcgcgt
      541 gcgcgaggaa cttcggctct cacagagagc aatggcggac cgcgtcgccg aagccgctcc
      601 tgcctcgaag atggcgcagg aggcgctgtt cgagtatcac ctctacaccc tcgcgcgccc
      661 gacgaccatc gccgacaacc agaccaagca ggtcgcgctg ctcagcgccc aggccgtgcc
      721 ggtcgtgaag gagctcgttc tccagggcag cgactactac taccgctcga gtgtcggcgg
      781 catcgggcag aagatgaagg tcggcgtgtt cgtgcagctc gagaaccgcg agtcttcgca
      841 cctcggcatg ccgatgccga agggcgtagt gcgcgtctac aagaaggacg gcgccggcaa
      901 cgcccagttc gtcggagagg atcgcatcga ccacacgccg aagaacgaga ccgtacgcct
      961 gaagctcggc gaagccttcg acctcaccgc cgacaagaag cagaccgact tcaagcgccg
     1021 cgagcccacc aaccgcgcga gctacgtgtt cgagagcgcc tacgagatcg tgctacgcaa
     1081 cgcgaagaag gaagcggcga gcgtcgtggt gcgcgagccc gtgccgggcg actggacgat
     1141 gctcgaggaa acccagcgcc acgccaaggt cgcggccggc actgccgagt ggcgcgtgca
     1201 cgttccagcc gagggaagca cgaccctgcg ctaccgggtc ctcgtgcgct actagccttc
     1261 taccggcaag acggtgatcc ggtactcatg gcgcagtttg cgcccaagta ctgggtcctc
     1321 gagctgcatg gtgaagcgca ccgccggccg gatgccgccc tttgccgcca gcgtcccgcc
     1381 aaacatcgcc cactggggct tcaagggata acggctcatg aggtcctcgg gagagcgcat
     1441 ggcgctaacc ggaccctcct ggtagagctc gctgtcggcc caggagcgca gcacgagcct
     1501 ctcccagtgc ggcttcacct cctcgtagcg ccacgagtcg cgtgataccg gcttggcgca
     1561 tagttgcttc gagagcgata cgccgatcgt ctcggccttg cgatcggtgt ggtcggagcc
     1621 aacggcgacg cgcaggtcgt cgtctctaat gaggacgaat tccacttcgc cgctggtgtc
     1681 cggcccggat acctggattt cctcggcatg gcaaaacagg ttcgccgcca cgcggtagaa
     1741 gatcggcgtc gtcttcggcg gcttgacgcc gatctcctcg agctccttga tgtgcttcct
     1801 gagcgctgct tcatcccgcc cggtccatcc cgcgatgaca agttccttta cgtgaaagct
     1861 catgccaccc ttttcgcttg cgatcgtgga atgtcgtagg tgcccatcag gtcgtcgacg
     1921 atcttgtgaa attcgttcca cggcgtctcg cggaaggcgt ggttgcgcac gataaacgag
     1981 gcgccggcgt agaacttctc gtactggagg tggcgccccg cgaactcgga gccgaccatg
     2041 tcccaggcga gcttgaacag cttcatgcgc tcgatcgccc cgctctgcgg ggtctggaag
     2101 taggtcgtga acttgagcgc gagctcctcg ttctcgacca cggagatgtc ggcgggcatc
     2161 tggaacacgc cgccgccgca cagctcgcgc aagcgctcga tgaagtcgct gtagttctgc
     2221 gtgcaccatt cgagcgcggc gtacatcatg cggcggttga agcagacgta gccgtcgggc
     2281 cagcgctcga gcgcctcgat ctggccgttc accatgccgc cgatcatgct ctcgaccgcc
     2341 gccatgtggc cgagcacctc cttcaccgcg ggcacctcga tggccccggt ggcgtgcgcg
     2401 atcttgctgc atacgccgag cagcagccgc atcttggacc agtagcgcac gttcgattga
     2461 tggttgccga agcaatggct cggcgtcttt acgtagatgt cgcgcgaaag gagagcgtcg
     2521 tcgtggacga acacgcgctc ccacggaacc ttgacctcgt cgcacagcac catgctgtcg
     2581 gtctcgtcga agcgccatgc gagcggcgcg tcgaactccg acgtggcctc gcgctcgaac
     2641 ggcttgcgcg accaaagcga caaccctttc gtcgcgatcg gcagcgagca ggtaatcgcc
     2701 tgcttcttct ggtctgccgc gagcggcagc acgttgccga tccagacttc gttggcgaac
     2761 accgcgcccg tggcgagcat tttcatgccc gagatcacga gcccgtcgtc ttcctcgcgc
     2821 accaccgaga gcgtcgggat gggaatgttc tgccgctggt agaactccgg gttgcgcgcc
     2881 gcctgaggtg ggaccacggc atacaccaca taggcgtcgg ttctcgctat gtctctatag
     2941 aagttcagca ggttctcggg ccgcccgcag ggcgctttga gctcctcggg cttcatcgcc
     3001 atgcccgtga cgaagctcgc gacatggtcg ggcgaacggc cgaacatgcc gtaggtgagg
     3061 tcggcgatca tgcggtggcc gcgagtgcgg cgcgcgaggt cctcgcgcgt cttcggctgc
     3121 aggaagtaga tcgagaaccg ctcgccgtcc tgctcgaagc tcatggcatc gcgctcggcg
     3181 agcttcatgt catagatcga ggccacggta cgcgccgcgt tcctgaacgc cgggtgcgtg
     3241 gtgacgtcgg cgacgcgctc cgagccgatg tagaccaccc ggccgtcgcg cagcgactct
     3301 agatgttcat ttccgctctt gagcatagta gtggatctcg tgaaggatgc agccggcgcg
     3361 ggaggtgaag gggccgtggt agacgccggg cgggcggcaa gcgtaggtgg gcgcgaagca
     3421 ctgggtgccg cccttgccct gcttgtcgtt gcccaccacg aggtcgccct gtacgaggta
     3481 cacctcctcc cagtggtcgt ggacgaaggg ctcggtggag aacgcgcccg gctcgatctt
     3541 gagaagccgc gtgcggcttc cggtcttctt cttctcgtcc aggtcggacg caaggatctt
     3601 ctggtaaaag cccgacggat atcccggtgg cacctcccag ccgctgttga gatccacgcg
     3661 cacgaactcg agatgctgct tcggtttcat cacgcttgct cctcgttgcc gcccagccag
     3721 ggcgagcgcc acacttcctc ggccttcatc gcctggttca ccacgctgcc ttcaaaggaa
     3781 acgctcatgc cgcctccgcc tcgtgcagca ggagctcgag catcgccacg gcgcgatcgc
     3841 cgcatgccac gagctcggcg ccgagcgtgc gcagctcgcg gaagcgcttc gcgtcgagcg
     3901 gctcgaacag cacgtcgttg acacggcgct ggtacggagc catgtgctcc agaagctgga
     3961 cacttttctc gttcatccgg agaaggacgc ggcggccgtc ggccggatcg gccgcctttt
     4021 cgaggattcc cgcggcgacc agcttattcg cctcgatggt gatgaaggcg ccagagcggt
     4081 gcatgcgcgc cgccacctcg ccgaccgaca agccatcggc gcggctcacc agcatgagaa
     4141 tctcgtactg cgcgcccgag atgccggcga tcgcggcaaa ggcatcacgg caggcgtcga
     4201 gcttgtgccc gtaagcgatg agatcgtgga tcaggcggcg gaactcgtca tccgagccgc
     4261 gcgtgagcag cgccgcgcgt gataccgtga gcggtggagt tttcttgcgg ggacgcgcca
     4321 tggggggcga aaattctccg cccgggcaat tacctttgtc aattagctaa atggccaaaa
     4381 cgggcgatct gcgcgaggcg ctcgcggcgc tcgaaagggg cgactggcag gcggcgcacg
     4441 ccatcgtgca ggcggacgaa gactcgcgcc tcgcctgctg ggcgcacggc atcgtgcacc
     4501 tcatggaagg cgatactgcg aatgcacgct actggtaccg cagggccggg cggccgttca
     4561 ggcaggacgt ggccgcggag atcgcggcgc tcgacgc
//
LOCUS       DASTPD010000150         6652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_693146, whole genome shotgun sequence.
ACCESSION   DASTPD010000150 DASTPD010000000
VERSION     DASTPD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693146"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..613)
                     /locus_tag="VFR57_05280"
     CDS             complement(146..613)
                     /locus_tag="VFR57_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0258813.1"
                     /translation="MKLSKDGWGGLAVLAVSLVLFSLTFELKPNRLVPIGPGFYPRAV
                     LVLTALLAAALLVLDLRRARGERSSTPAPNYAAVVLHFAVFGVYVAALPWLGFRIATF
                     AYVTASNALMDRPRSARGWLRVVVIALATVLVTYFAFERYLLVLLPRGRWTDF"
     gene            complement(610..1548)
                     /locus_tag="VFR57_05285"
     CDS             complement(610..1548)
                     /locus_tag="VFR57_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258814.1"
                     /translation="MRKLLVATLLGLVSAAAQAAYPERPIRMIVPWAAGGDTDNIFRP
                     FAPLLQKQIGQTVVIANVGGASGTRGAKEAKDAPPDGYTLYAVHEYIHSTYYAGVADV
                     QHSDFEPVCLISSTPSVLTASPKTPWKSWQDLAADAKARPGQISVGATLASTSHFFPA
                     LIEKATGLKFKYVSYEGLAPRMNAILGGHIDLTDSNLTQKGKVEAGQLKFLAIATEKR
                     HPEMPSVPTLKELGVNVVYDVNRGIMAPKGTPADILAKLGAACAAAAKEPEFAKAMRL
                     QGTDVRYLGRAEYARWLKETDDLNRTLAKDLGLLKR"
     gene            complement(1631..2659)
                     /locus_tag="VFR57_05290"
     CDS             complement(1631..2659)
                     /locus_tag="VFR57_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0258815.1"
                     /translation="MKRVVLIGAGHAHLVVLRSLAEKPLYNARIALVSPHAKQVYSGM
                     LPGILAGHYRRAEAEVDIATLAERGQVEFVPGKVQRLDPDKKTVTLESGEDSGYDLAS
                     INAGSLVGASLPGAERALPVKPYEEFLSRLRIPERVAIVGAGAAGAEIAMAVRHRGAQ
                     VTLYSENASISARVVRQLRRRKVDYRPGMPVTAIEPGPLVITGLARQEFDCVVLATGA
                     APLPWLRQSGLRTCARGFVLVDSTLRSVSHPEVFAAGDCATLADSPHPKSGVYAVRHG
                     EALIENLRRRVAHADLEPYAPQEKALALITCGARYAIAEHGEWSAQGWWVWWWKDRID
                     RRWIRSFL"
     gene            complement(2656..4296)
                     /locus_tag="VFR57_05295"
     CDS             complement(2656..4296)
                     /locus_tag="VFR57_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0258816.1"
                     /translation="MPDRYDELYRSFRWDVPERYNMARACCGQWAGDRARFALYWEDE
                     SGATAAYSFWDIQQAANRLSNALAALGVKRGERVAIILPQRPETAIAYVAVFQMGAIA
                     LPLSHLFGPDALEYRMEHAEASVAIVEPTTIGNLRAIRDRLTHLRHVIGVDLHEGGIQ
                     GWKDLLQKASARFTPMDTKADDPALIIYTSGTTGPPKGALKAHRVLLGNAPGFVHSHD
                     FFPQPGDMFWSPADWAWTGGLMDALLPAWLFGMPILGYRGRFDAEKAYYLLEKYGVRN
                     SFLFPTALKLMMKAFPSPKAKFDISLRSIMSAGESVGVTVFEWAKAELGVTINEMFGQ
                     TEINYVVGNCQSAWPAKPGSIGRPYPGHRVAVIDDKGNEARRGELGEIAVNRRFNGQP
                     DPVFFLEYWKNENATREKFIGDWGCTGDQAKMDEDGYLWYQGRSDDVIKSAGYRIGPA
                     EIESCLVKHPAVLNAAVIGKPDATRGSIVKAFIVLQPGKLPSEPLIEQIQEHVRGRLA
                     PYEYPREIEFIEALPMTTTGKVQRKELRKLEEAKMQKA"
     gene            4295..4966
                     /locus_tag="VFR57_05300"
     CDS             4295..4966
                     /locus_tag="VFR57_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0258817.1"
                     /translation="MRGFYTSAMAAEAMVFDAYGTLYDVHSVAARCEMFWPGKGTELS
                     TLWRAKQLEYTWQRSLMQRYVPFSTVTREALAYACETLGLELDRVREQALMQEYLRLA
                     TYPDVQETLKKLKSRKQAILSNGSPDMLLPLVGHSGLRFDAVISVDELKIYKPAPQVY
                     ELAVRRLNTEKTRIAFVSSNCWDAIGAKSYGFRVYWINRARAPVDRLGFRPDAILASL
                     NEVLQ"
     gene            4950..5477
                     /locus_tag="VFR57_05305"
     CDS             4950..5477
                     /locus_tag="VFR57_05305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0258818.1"
                     /translation="MKFCSNCASPVMKRVPPGDSLPRWVCDHCGEIHYQNPRLVVGSI
                     PAHEGKVLLCRRAIEPRYGYWTLPAGFMENDETTAQGALRETLEEAGARIELDAPFSM
                     ISVPRVNQVHLFYRARVLELGFKPGEETLEVALWEESNIPWPEIAFRTVGLTLKHWFA
                     DRGNGAFGFHAEDLQ"
     gene            5505..>6652
                     /locus_tag="VFR57_05310"
     CDS             5505..>6652
                     /locus_tag="VFR57_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0258819.1"
                     /translation="MKKVLLLAALYAPAALAQAPAAAPKIDAANTAWMLTAAVLVLFM
                     TLPGLALFYAGLVRTKNVLSVLMQCFAITCVVTLAWLVVGYSIAFGEGNAFWGGLGKS
                     FLSGIQVASVKGSIPESVFAMYQLTFAIITPALVVGAYAERVRFGGMLLFSLLWLLIV
                     YCPVAHWVWGEGWLQKRGLLDFAGGTVVHLNAGVAALVCALVLGRRRGFPETAMAPHN
                     MTMAVTGAAMLWVGWFGFNAGSALAADGAAGMAMLVTHIGAATGAFAWMVCEWLRYDK
                     PSVLGIVTGMVAGLGTITPASGFVGPIGALIIGGAAGVACFFATNYMKRALNIDDSLD
                     VFPVHGVGGLLGTLLTGILVSSTFGGIGYADKVTMGEQFVTQLVGVLAV"
BASE COUNT         1091 a         2202 c         2191 g         1168 t
ORIGIN      
        1 gcagcaccac gatgatcgtc atcgtgatcg tcaccccggg cagcgcgccg ccgatgactc
       61 cggcgagagt gccgaggaag agcggaagca ggtatttcac ctgcacgacg ttgacgatgc
      121 cggccgcgag gagctcgtac atgggctaga agtcggtcca gcgcccgcgc ggcaggagca
      181 cgagcagata gcgctcgaag gcgaagtacg tgacgagcac cgtggcgagt gcgattacca
      241 ccacgcgcag ccacccccgc gcgctccgcg ggcgatccat cagcgcgttg ctcgcggtga
      301 catacgcgaa cgtggcgatg cgaaagccga gccacggcag cgccgccacg tagacgccga
      361 acaccgcgaa gtgcagcacc accgcggcgt agttcggtgc cggcgtgctc gagcgctcgc
      421 ctctcgcacg ccgcagatcg agcacgagca gggcggccgc gagcagcgcc gtcagcacga
      481 gcaccgcccg cgggtaaaac ccggggccga tcggcaccag ccgattgggc ttgagctcga
      541 aggtaaggct gaaaagcacg aggctcaccg cgagtacggc gagcccgccc cagccgtcct
      601 tcgacagctt caacgtttca gtaaaccaag atctttggcg agcgtacggt taaggtcgtc
      661 ggtttccttc agccacctcg cgtactcggc gcgccctagg tagcgcacat cggtgccctg
      721 cagccgcatc gccttggcga attccggctc cttcgccgcg gcggcgcacg ccgcgccgag
      781 cttggcgagg atgtcggcgg gcgtgccctt tggcgccatg atgccgcggt tcacgtcata
      841 gaccacgttg acaccgagtt ccttcagcgt gggcacgctc ggcatctcgg ggtggcgctt
      901 ttcggtggcg atggcgagaa acttgagctg cccggcctcg accttgccct tctgcgtaag
      961 gttggagtcc gtgaggtcga tatggccgcc aaggattgcg ttcatgcgcg gcgcgaggcc
     1021 ctcgtaggag acgtatttga acttcagccc ggtcgctttc tcgatcagcg cgggaaagaa
     1081 gtggctggtg gaggcgagcg ttgcgcccac gctgatctgc ccgggccgcg ccttggcgtc
     1141 ggcggcgagg tcctgccagc tcttccaggg cgtcttcggg ctcgcggtaa gcaccgaggg
     1201 cgtcgacgag atcagacaga ccggctcgaa gtcgctgtgc tgcacgtcgg ccacgccggc
     1261 gtagtaggtg gaatggatgt actcgtgcac cgcgtagagg gtgtagccgt ccgggggcgc
     1321 atccttcgct tccttcgcgc ctcgcgtgcc ggaggccccg ccgacgttgg cgatcaccac
     1381 agtctggccg atttgcttct gcagcagcgg tgcaaacggc cggaaaatgt tgtcggtatc
     1441 gccgccagcc gcccacggca cgatcatccg gatcggccgc tcggggtacg ctgcctgggc
     1501 cgcagccgat acgagcccga gtagcgtcgc aactaggagc tttctcatcg tttcctcctc
     1561 caaagagccg ccatgatatg acagcgaaat catccctgca gcgcaggcgc gcggccggcg
     1621 cgcgcgcgtg ttacagaaac gagcgtatcc agcggcggtc gatgcggtct ttccaccacc
     1681 aaacccacca tccctgcgcg ctccactcgc cgtgctccgc gatcgcatag cgcgcgccgc
     1741 acgtgatgag cgcaagcgcc ttctcctgcg gcgcgtacgg ctccaggtct gcatgcgcga
     1801 cccggcgacg caggttctcg atcagggctt cgccgtggcg cacggcatag acgcccgact
     1861 tgggatgcgg cgaatcggcg agcgtcgcgc aatcgcccgc cgcgaacacc tcgggatgcg
     1921 agacgctgcg cagcgtgctg tcgaccagca cgaagccgcg cgcgcacgtc cttagccccg
     1981 attgccgcag ccacggtagc ggcgcggcgc cggtcgcgag cacaacgcag tcgaactcct
     2041 ggcgcgcaag gccggtgatg accagcggcc cgggctcaat ggcagtgacc ggcatgccag
     2101 gcctgtagtc caccttgcgt cggcgcagct gccgcaccac gcgagcgctg atcgaggcat
     2161 tttccgagta cagggtcacc tgcgcgccgc gatggcgcac tgccatcgcg atctcggcgc
     2221 ccgccgcgcc ggcacccacg atcgcgacgc gctcggggat gcggagccgc gacagaaact
     2281 cttcgtaggg cttgaccggc agcgcacgct cggccccggg aagcgacgcg ccaacgagcg
     2341 agccggcgtt gatcgaagca aggtcgtagc ccgagtcctc gccgctttcg agggtaaccg
     2401 ttttcttatc ggggtccaag cgctgcactt tgcccggcac gaactcaacc tggccgcgct
     2461 cggcgagtgt ggcaatgtcc acctcggctt cggcgcgcct atagtgcccg gccagaatgc
     2521 caggcagcat gcctgaatag acctgcttcg cgtgcggact gaccagcgcg atgcgggcgt
     2581 tgtaaagcgg cttctcggcc aagctgcgca ggacgacgag gtgcgcgtgg cccgcgccga
     2641 tgaggacaac tcgcttcacg ccttctgcat cttggcttct tcgagcttac gcaactcttt
     2701 tcgttgcacc ttgccggtgg tcgtcatcgg caacgcttcg atgaactcga tctcgcgcgg
     2761 gtattcgtag ggtgcaaggc gaccgcggac gtgttcctgg atttgttcaa tgaggggctc
     2821 ggacggcagt tttcccggct gcaagacgat gaaagccttt acgatggagc cgcgcgtggc
     2881 gtcgggcttg ccgatcaccg cggcgttcag caccgccggg tgcttcacca ggcagctttc
     2941 gatctccgcc ggcccgatgc ggtagcccgc gctcttgatc acgtcgtcgg aacggccctg
     3001 gtaccagagg tacccgtcct cgtccatctt cgcctgatcg ccggtgcagc cccagtcgcc
     3061 gatgaacttt tcgcgcgtcg cgttctcgtt cttccagtac tcgaggaaga acaccgggtc
     3121 cggctggccg ttgaagcggc ggttgaccgc gatttcgccg agctcgcccc ttcttgcctc
     3181 gttccccttg tcgtcgatga ctgcgaccct gtggccggga tacggccggc cgatcgagcc
     3241 gggcttcgcc ggccacgcgc tctggcagtt gccgaccacg taattgatct cggtctggcc
     3301 gaacatctcg ttgatcgtga cgcccagctc ggctttcgcc cactcgaaaa ccgtcacgcc
     3361 caccgactcg cccgcgctca tgatcgagcg cagcgaaata tcgaacttcg ctttcggcga
     3421 aggaaaggct ttcatcatca gcttgagggc ggtcgggaag aggaacgaat tgcgcacacc
     3481 gtacttctcg agaaggtagt acgccttctc cgcgtcgaag cgcccgcggt agccgaggat
     3541 cggcatgccg aagagccacg ccggcagcag cgcgtccatc aggccgccgg tccacgccca
     3601 atcggcgggc gaccagaaca tgtcgccggg ctgcggaaag aaatcgtgcg agtgcacaaa
     3661 gcctggcgcg tttcccagca gcacgcggtg cgccttcagc gcgcctttcg gcggccctgt
     3721 agtgccgctc gtatatatga tcagggcagg gtcgtccgct ttggtatcca tcggcgtgaa
     3781 gcgcgcgctc gccttttgga gcagatcttt ccacccttgg ataccgcctt cgtgaaggtc
     3841 tactccgatc acatgccgca agtgcgtcag gcgatcccgg attgcgcgca ggttcccgat
     3901 ggtggtcggc tcgacgatcg caaccgacgc ttcggcgtgc tccatgcggt actcaagcgc
     3961 gtcgggaccg aacagatgcg agagcggcag cgcgatcgcg cccatctgga acacggcgac
     4021 ataggcaatc gcggtctcgg gccgctgcgg caggatgatc gcgacccgct cgccgcgctt
     4081 cacgccaagc gcagccagcg cgttggacag gcggttcgcc gcctgctgga tgtcccagaa
     4141 ggaatacgcc gcggttgcgc ccgactcgtc ctcccagtag agcgcgaaac gcgcacgatc
     4201 gccggcccat tgcccgcaac aggcgcgcgc catgttgtag cgctccggca cgtcccagcg
     4261 gaagctccgg tagagctcgt cgtagcggtc cggcatgcgg ggattctata cttcggccat
     4321 ggcggcggag gcgatggtct tcgatgcgta cggcacgctt tacgacgtgc actcggtcgc
     4381 ggcgcgctgc gagatgttct ggccgggaaa gggaaccgag ctctcgacgc tgtggcgcgc
     4441 gaagcagctc gagtacacct ggcagcgcag cctgatgcag cgctacgtcc cgttctcgac
     4501 ggtgacgcgc gaggcgctcg cgtatgcctg cgagacgctc ggcctcgagc tcgaccgcgt
     4561 ccgggaacag gcgctcatgc aggagtacct gaggctcgcg acctaccccg atgttcaaga
     4621 aaccctgaag aaactcaaat cgcgtaaaca ggcgattctc tccaatgggt cgccggacat
     4681 gctgctgccg ttggtcgggc actcggggct tcgcttcgac gccgtgatca gcgtcgacga
     4741 gctgaagatc tataagcccg ccccgcaggt ttacgagctc gcggtgagga ggctgaacac
     4801 ggaaaagacg agaatcgcct tcgtgtcctc gaactgctgg gacgcaatag gcgcgaagtc
     4861 ttacggtttc cgggtttact ggatcaaccg cgccagagcg cctgtggacc ggctcgggtt
     4921 caggccggat gcgatcctcg cctcgctgaa tgaagttctg cagtaactgc gcctcgccgg
     4981 tgatgaagcg cgtgccgccg ggcgacagcc tgccgcgctg ggtctgcgac cactgcggcg
     5041 agatccatta ccagaatccg cgcctcgtgg tgggctcgat cccggcgcac gagggcaagg
     5101 tactcctctg ccggcgcgcg atcgagccgc gctacggcta ctggacgctg cccgcgggct
     5161 tcatggagaa cgatgagacc acggcgcagg gggcgttgcg cgagacgctc gaagaggccg
     5221 gggcccggat cgagctcgac gcgcccttca gcatgatctc ggtgccgcgc gtgaaccagg
     5281 tgcacttgtt ttaccgcgcg cgcgtgctgg agctcgggtt caagcccggc gaggagacgc
     5341 tcgaggtcgc gctctgggaa gaatcgaaca tcccgtggcc cgagatcgcg ttccgcactg
     5401 tcggcttgac gctcaagcac tggttcgccg atcgcggcaa cggcgctttc ggcttccatg
     5461 cggaagacct acaatgaaaa aaccaaaata aaggggaggc tggaatgaaa aaagttctgc
     5521 tcttggctgc gctctacgcg cctgcggcgc tcgcccaggc gcctgcggcc gcgccgaaga
     5581 tcgacgccgc gaacaccgcc tggatgctca cggcggcggt gctggtgctg ttcatgacgc
     5641 tgcctggcct cgcgctcttc tatgccgggc tggtgcgcac caagaacgtc ctttcagtcc
     5701 tgatgcagtg cttcgcgatc acgtgcgtgg tcacgctggc ctggctcgtc gtcggctaca
     5761 gcatcgcgtt cggcgaaggc aacgccttct ggggcggcct cggcaagagc ttcctctcgg
     5821 gcatccaggt ggcgagcgtc aagggaagca tcccggagag cgtgttcgcg atgtaccagc
     5881 tcaccttcgc gatcatcacc cccgcactcg tggtgggcgc ctatgccgag cgcgtgcgtt
     5941 tcggcggcat gctgctcttc agcctgctgt ggctgcttat cgtctactgc ccggtcgcgc
     6001 actgggtctg gggcgagggc tggctgcaga agcggggcct tctcgacttc gcgggcggca
     6061 ccgtggtcca cctgaacgcc ggtgtggcgg cgctcgtctg cgcgctcgtc ctgggccggc
     6121 gtcgcggctt tcccgagacg gcgatggcgc cccacaacat gaccatggca gtgaccggcg
     6181 cggcgatgct ttgggtcggc tggttcgggt tcaacgccgg cagcgcgctc gccgccgacg
     6241 gcgccgccgg catggcgatg ctcgtgacgc acatcggcgc cgcaaccggc gcgttcgcct
     6301 ggatggtttg cgaatggctg cgctacgaca agccctcggt gctcggcatc gtgaccggca
     6361 tggtcgccgg cctgggcacc atcacgcccg cctcgggctt cgtcggcccg atcggcgcgc
     6421 tcatcatcgg cggggcggcg ggggtcgcct gctttttcgc gacgaactac atgaagcgcg
     6481 cgctcaacat cgacgactcg ctcgacgtgt tcccggtgca cggggtcggt ggcctcctcg
     6541 gcacgctact caccgggatc ctcgtttcca gcacgttcgg cggcatcggg tacgcggaca
     6601 aggtcaccat gggcgagcag ttcgtcaccc agctcgtcgg cgtgctggcg gt
//
LOCUS       DASTPD010000151         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1510520, whole genome shotgun sequence.
ACCESSION   DASTPD010000151 DASTPD010000000
VERSION     DASTPD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510520"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFR57_05315"
     CDS             <1..730
                     /locus_tag="VFR57_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEU0258820.1"
                     /translation="MNTVELMPTDEIKLGEMAVFVGRNYVLSVRRRAERGFREVRTNA
                     ERHPDLLRWGPGFVLYALMDAVVDRYFPVLEAIETELEILEEGLFIPGGSPRENIESL
                     YHVKQELTTLKHAAGPLLENVSKLFGGRVPQACAGLGEYFRDVYDHLVRINQSIEAAR
                     DTVGTGIQVALAMVATRQGEITRMLAAYAALVAVPTMIAGIYGMNFKHMPELDWAFGY
                     PMVLGAIVGIDAFLFSRFRKAGWL"
     gene            778..2493
                     /locus_tag="VFR57_05320"
     CDS             778..2493
                     /locus_tag="VFR57_05320"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEU0258821.1"
                     /translation="MEFWNLAILVAAALLLVSIVVSDISSRLGAPLLLVFLVLGMLAG
                     EDGPGGIRFDDFETAYVIGTLSLAVIIFDGGLRTSRDTFRVALWPAVSLATVGVLVTA
                     GLVGLLAAWVLGLALLEGMLLGAIVGSTDAAAVFALLRTQGAALKRRVASTLEIESGS
                     NDPMAVFLTVAILELLAVGKTGLEASTALSFGMQFAIGALLGVAGGRLLVWLINRLRL
                     ISGLYPLLAAAGGILIFAVAAQLGGSGFLAIYLAGLVLGNSRLQARQTILRVHDGLAW
                     LSQIVMFLILGLLLTPSQLVGIAVPALIIAAGLIVLARPIAVVVSLLPFSFPWREQLY
                     IGWVGLRGAVPIVLALFPAMYGLDNARLYFNVAFFVVLVSLLLQGWTIAPAARWLQLE
                     APPQIEPVQRVTVDMPGHFEHEILGFEVKPGSMVAGRTLGDLVMPEGMKIMAAIRDGV
                     PQDPAPGLDFRPGDFIYFLAQPQAIAHLGKLFDPHRVPDRLEEHRYFGDFVLNGDAML
                     EDLAAAYGLEAPEQAAGKSLADYLDRAFHGRAVVGDRVRIGAAELVVREIENGRISRV
                     GLRLR"
     gene            complement(2497..>4186)
                     /locus_tag="VFR57_05325"
     CDS             complement(2497..>4186)
                     /locus_tag="VFR57_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEU0258822.1"
                     /translation="SLKYVVLVMRADNRGEGGTMALIALASAAVRKRPQWRIFILTVG
                     LFGASLFFGEAVLTPAISVVSAVEGLEVGTSAFKPWVLPIVAVVLIALFSFQRHGTAV
                     VGAFFGPVCMLWFIALGAVGIWQIAQNPVVLQAVDPRHAFGFLTSHGFASFVVMGAVL
                     LAFTGAEALYADMGHFGKWPIRAAWFGLVLPALMLNYFGQGALLIANPAALDNPFYHA
                     FPGWALYPMVGLATAATIIASQATITGAYSLTRQAIQLGFLPRVNIIQTSARQYGQIY
                     IPAINWVLLILVLAAVFGFGSSSELASAYGVAVTGTMTVTTILTFFVIRYGWGYPLWL
                     CLFATGFFFLIDVTFFSAALLKIFEGGWFPLVLGISMFTIMTTWRRGREVMFERLSEA
                     SVPLLPFLKSLFADPPLRVPGTAVFLTATPDATPHALLHNLNHNKVLHERVVFLTVEI
                     RDVPWVSFEERVTCERLGHGCWRVRVRYGFMNRPDVMRALEVCGALGLEFDFMQTSFF
                     LSRQQVVPAPGGISTMARWRERMFAAMARNAGNITDYFNIPTNRVIELGTKVEV"
BASE COUNT          719 a         1351 c         1404 g          712 t
ORIGIN      
        1 gatgaatacg gtggagctca tgcccaccga tgaaatcaag ctcggcgaaa tggcggtgtt
       61 cgtcggccgg aactacgtgc tgtccgtgcg caggcgcgcg gaacgcggtt tccgagaagt
      121 gcgtacgaac gccgaacgcc accctgacct gctgcgctgg gggccgggct tcgtgcttta
      181 cgccctgatg gacgctgtcg tagaccgcta tttcccggtg ctcgaggcaa tcgagacgga
      241 gctcgagatt ctcgaggagg ggctgttcat ccccggcggc tcgccgcgcg agaacataga
      301 gtccctgtat cacgtaaagc aggagctcac gaccctcaag cacgctgccg gtccgctgct
      361 cgagaacgtg agcaagctct tcggcggccg cgtgccgcag gcctgcgccg gcctcggcga
      421 gtacttccgc gatgtctacg accatttggt ccggatcaac cagtcgatcg aggccgcgcg
      481 cgataccgtg ggcacgggca tccaggtcgc gctcgccatg gtggcgaccc gccaaggtga
      541 aatcacgcgc atgcttgccg cctacgctgc cctcgtcgcc gtgccgacga tgatcgcggg
      601 gatctacggc atgaacttca agcatatgcc cgagctcgac tgggccttcg gctatccgat
      661 ggtccttggc gcgatcgtcg ggatcgacgc ctttcttttc tcgcgcttca ggaaagcggg
      721 ctggctgtaa gcaacagcac tccaaaaagg tttgcccctt tctgctagct tcgcggggtg
      781 gagttctgga acctcgcgat cctggtcgcc gccgcgctgc tgcttgtcag catcgttgtc
      841 agcgacattt cgtcgcgcct cggcgcaccg ctgctgctcg tattcctggt cctgggcatg
      901 ctggcgggcg aggacggtcc ggggggaatc cgcttcgacg atttcgagac cgcctacgtc
      961 atcggaactc tgtccctcgc ggtcatcatc ttcgacggcg gcttgcgcac cagccgcgat
     1021 acgttccgcg tcgcgctctg gccggcggta agcctcgcta ccgttggagt gttggtaacg
     1081 gccggcctcg taggcctgct cgccgcctgg gtgctggggc tcgccttgct ggaaggcatg
     1141 ctgctcggag cgattgtggg ctcgaccgat gcggcggccg tgttcgctct cctgcgcacg
     1201 caaggcgccg cgctgaaaag gcgcgtcgcc tccacgctcg agatcgagtc aggcagcaac
     1261 gaccccatgg cggtgtttct cacggttgcg atcctcgagc tgctggcagt cggcaagacc
     1321 gggctcgaag cctcgacggc gctgtccttc gggatgcagt tcgcgatcgg ggcgctactc
     1381 ggcgttgcgg gtgggcggct tctggtctgg ctcatcaacc ggctgcggct gatcagcggc
     1441 ctctacccgc tgctcgccgc cgccggcggc atcctgatct tcgccgtcgc cgctcagctg
     1501 ggcggcagcg gcttcctcgc gatctacctc gcaggcctgg tgctcgggaa ttcgcgtttg
     1561 caggcccggc agaccattct gcgggtgcac gacgggctgg cttggctcag ccagatcgtc
     1621 atgttcctga tcctcggcct gctgctgacg ccttcgcagc tcgtgggtat cgccgtgccg
     1681 gcgctgatta tcgccgcggg cctcatagtg ctcgcgcggc cgatcgcggt ggtcgtcagc
     1741 ctgctaccct tttccttccc ctggcgtgag cagctctaca tcggctgggt cggcctgcgc
     1801 ggcgcggtgc cgatcgtgct agccctgttc cccgcgatgt atggtttgga caatgcacgg
     1861 ctttacttca acgtagcttt tttcgtagtt ctggtatcgc tcctgctgca gggatggacg
     1921 atcgcgccgg cggcgcgctg gctgcagctc gaggcgccgc cgcagatcga gccggtgcag
     1981 cgcgtcacgg tcgacatgcc ggggcacttc gagcacgaga ttctcggctt cgaagtgaag
     2041 cccggtagca tggtagccgg ccgtacgctg ggcgacctcg tcatgcccga ggggatgaag
     2101 atcatggcgg cgatccgcga cggcgtgccg caggacccgg caccgggact cgatttccgg
     2161 cccggcgatt tcatctattt cctcgcccaa ccgcaggcta tcgcgcacct agggaagctc
     2221 ttcgacccgc accgcgtgcc cgaccgcctg gaggagcatc gctacttcgg cgatttcgtc
     2281 ctgaacggcg atgccatgct ggaagacctc gccgccgcct atgggctgga agctcccgaa
     2341 caagcggcgg gcaagagcct cgcggactat ctcgaccgcg ccttccacgg ccgcgccgtg
     2401 gtcggcgacc gcgtgcgcat cggcgcggcg gagctcgtgg tgcgcgagat cgagaacggc
     2461 aggatcagcc gcgtgggcct gcgcctgcgc tgaaaatcag acctccacct tcgttccgag
     2521 ctcgatcacg cggttggtag ggatgttgaa gtagtcggtg atgttgccgg cgttgcgcgc
     2581 catcgcggcg aacatgcgct cccgccagcg cgccatagtg gagatgccgc caggcgccgg
     2641 cacgacctgc tggcgcgaga ggaagaacga ggtctgcatg aagtcgaact caaggccgag
     2701 cgccccgcac acttcgagcg cgcgcatgac gtcgggacgg ttcatgaagc cgtagcgcac
     2761 acgcacgcgc cagcagccgt gcccgaggcg ctcgcaggtg acgcgctcct cgaacgacac
     2821 ccagggcacg tcgcggatct cgacggtgag gaacaccacg cgctcgtgca gcaccttgtt
     2881 gtggttcagg ttgtggagca gcgcgtgcgg cgtggcgtcg ggcgtcgcgg tgaggaacac
     2941 tgcggtgccg gggacgcgca gcggcgggtc ggcgaagagc gacttgagga agggcagcag
     3001 cggtaccgac gcctcgctca ggcgctcgaa catcacctcg cggccgcggc gccaggtggt
     3061 catgatggtg aacatcgaga tgccgaggac gagcgggaac cacccgccct cgaatatctt
     3121 gagcagcgcc gccgagaaga acgtgacgtc gatgaggaag aagaagccgg tggcgaagag
     3181 gcagagccag agcggatagc cccagccgta gcggatgacg aagaaggtga ggatggtggt
     3241 caccgtcatg gtgccggtga cggccacgcc atatgcggag gcaagctcgc tggacgagcc
     3301 gaagccgaac accgcggcaa gcacgaggat gagcagtacc cagttgatcg ccggaatgta
     3361 gatctggccg tactggcgcg cggacgtctg gatgatgttt acccgaggca ggaagccgag
     3421 ctggatcgcc tggcgcgtga gcgaatacgc gcccgtgatc gtcgcctgcg aagcgatgat
     3481 ggtcgccgcg gtggcgaggc cgaccatcgg gtagagcgcc cagcccggaa acgcgtgata
     3541 gaacggattg tcgagcgccg cgggattcgc aatgagcagc gcgccctggc cgaagtagtt
     3601 gagcatcagc gccggcagca ccaagccgaa ccacgctgcg cggatcggcc acttgccgaa
     3661 gtgccccatg tcggcgtaaa gcgcctccgc gccggtgaag gcgagcagca ccgcgcccat
     3721 gacaacgaac gaggcgaatc cgtggctcgt gaggaagccg aacgcatggc gcggatccac
     3781 cgcctgcagc accaccgggt tctgggcgat ctgccagatg cctacagcac cgagcgcgat
     3841 gaaccagagc atgcacaccg gcccgaagaa cgcccccacg acagcagtgc cgtgccgttg
     3901 gaacgagaaa agagcgatca ggactacggc cactatcggc agcacccagg gcttgaaggc
     3961 gctcgtgccg acctccagcc cctcgaccgc ggaaacaacc gagatcgccg gcgtaagcac
     4021 cgcctcgccg aagaacagcg aggcaccgaa caatcccacg gtaaggatga aaatccgcca
     4081 ctgcgggcgc ttgcgcactg cagcgctcgc aagcgcaatg agcgccatgg tgccgccctc
     4141 accccggttg tcggcgcgca tcacgagcac tacgtacttg agcgac
//
LOCUS       DASTPD010000152         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1102115, whole genome shotgun sequence.
ACCESSION   DASTPD010000152 DASTPD010000000
VERSION     DASTPD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102115"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFR57_05330"
     CDS             complement(<1..717)
                     /locus_tag="VFR57_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258823.1"
                     /translation="MRRLLLLGALAGLVGYPLLDESATSAGLAFAQTEGAETRAAAKA
                     NAAERLELPERRGLTRTQGELFGAPPPPPKPITKKAQPPAPVAAAPVAPPVPYRFAGK
                     VRKGSEEEVLISKGDAVFPVKAGDTLDGMYKVESINAERIELVYLPLGTRDRIVVSSA
                     LDAERAAQSPLAAAPAPVPAAPATASASGATSAGPAQLRWEGPERVAAGESFSVTLRV
                     STTEPLRAAPMQLRFAPDVLQ"
     gene            complement(754..1515)
                     /gene="xth"
                     /locus_tag="VFR57_05335"
     CDS             complement(754..1515)
                     /gene="xth"
                     /locus_tag="VFR57_05335"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0258824.1"
                     /translation="MRIATWNVNSLRIRLPHLLDWLRSREPDVMCLQETKTEDHNFPL
                     AEVQAAGYHAVYCGQKAYNGVAILARTALTDVQHGIPHFSDDPKRVIAASVGDIRVLC
                     LYAPNGEAPGTDKYAYKLRWYQALTSWLQAELPRLPRLAVLGDLNIAPEPRDVHDPKR
                     WEGKIHFSEPELAAFQKVLKVGLKDAFRLFDQPEKQFTWWDYRLDAFQRGWGLRIDHI
                     LLSPQLAAICKACGIDTEPRKRERPSDHAPVSADL"
     gene            complement(1516..3567)
                     /locus_tag="VFR57_05340"
     CDS             complement(1516..3567)
                     /locus_tag="VFR57_05340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0258825.1"
                     /translation="MLPQENNPLLDFSGLPRFAEIKPAHVTPAIELLLSDARSTVKAA
                     EKSAPSWDAFVAPLEDANERLGRAWGQVAHLHAVMDSPELREVYNANLPKVTSYWTEL
                     GQNLQLFEKYKALRYSNEFSTLSSPRKKIVENALRDFRLGGAELPPDKKKRYAEIQEE
                     LARLSAKFSENLLDATNAFSIYVTKDETSGIPDDVLQAAREAAHKDGKDGWKFTLHMP
                     SYLPVMQYADERLLREKMYYASSTRAAEFGNPQWDNAPLIAKIVPLRRELAQLLGYCD
                     FAEVSLVPKMAESASQVLAFLDDLARRARPFAERDVLELREFSRTELGIPDLQAWDVA
                     YASEKLRLKRYSFSDQEVKQYFPEDIVERGLFRVAETLYGVKIVESQAPVWHPDVRFF
                     EVRDPNGTAIGQFYLDLYARETKRGGAWMDDAISRRRRAERIQIPVAYLNCNFSRPVG
                     GKPALFTHDEVLTLFHEFGHGLHHLLTQVEDLGVSGISGVEWDAVELPSQFMENFCWE
                     WDVLCGMTRHVDTGQPLPRQLFEKMLAAKNFQSGLAMLRQIEFAVFDMRLHSDFDPAS
                     GRSALDLLQEVRRQVAVLFPPAYNRFPNSFGHIFGGGYAAGYYSYKWAEVLSADAYSA
                     FEETGAAKGIILDPATGARFRDEILAVGGSRPAAESFRAFRGREPNVAALLRHNGMIA
                     A"
     gene            complement(3536..>3877)
                     /locus_tag="VFR57_05345"
     CDS             complement(3536..>3877)
                     /locus_tag="VFR57_05345"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102977.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU0258826.1"
                     /translation="PVALLLLQKDASVTICHSKTPRIETITRQADILVAALGRAKLVG
                     ADMVKPGACVIDVGVNRLPDGKLAGDVDFDAVKEIAGWITPVPGGVGPMTIAMLLENC
                     LRAAAREQSAS"
BASE COUNT          667 a         1241 c         1270 g          699 t
ORIGIN      
        1 ctgcagcacg tccggagcga atcgaagctg catcggcgcg gcgcgcagcg gctccgtagt
       61 gctaacgcga agcgtgacgc tgaagctttc gccggcggcc acgcgctctg ggccttccca
      121 acgaagctgc gcgggtcctg cgcttgtcgc tccgctcgcg ctcgccgtgg ccggcgcggc
      181 aggcacaggc gcgggagcag ccgccagcgg ggactgtgct gcccgctcgg catcgagcgc
      241 ggagctcacc acgatgcggt ccctagtgcc aagggggagg tagacaagtt cgatccgctc
      301 ggcgttgatc gactccacct tgtacatgcc gtcgagcgtg tcgcctgcct ttaccggaaa
      361 caccgcgtcg cccttcgaga tcagcacctc ttcttccgat cctttgcgaa ccttgccggc
      421 gaatcggtag ggcaccggcg gcgcgaccgg cgcggcggca accggtgcgg gcggctgcgc
      481 cttcttcgtt atcggcttcg gcggcggcgg aggagcgccg aagagttcgc cctgtgtgcg
      541 ggtaaggccg cggcgctcgg gcaactccag gcgctcggcc gcattcgcct tggccgccgc
      601 gcgcgtctcg gcgccctcgg tctgggcgaa cgccagcccg gcgcttgtcg cggactcatc
      661 gagcagcgga tacccgacga gtccagcaag ggccccgaga agcaacagcc tgcgcattcg
      721 cactcgcatt tcccctacgg acgcctccgg attctaaagg tctgcgctca ccggggcatg
      781 atcagaagga cgttcgcgct ttcgcggttc ggtgtcaatc ccacaggcct tgcagatggc
      841 ggcaagctgg ggggagagca ggatgtggtc gatgcgcagc ccccagccgc gctggaaagc
      901 atccaggcgg taatcccacc aggtgaactg cttttcaggc tggtcgaaca gccgaaacgc
      961 gtccttgagg cccactttca gcactttctg gaaggcggca agctcgggct cggagaagtg
     1021 gatcttgccc tcccagcgct tggggtcgtg gacgtcccgc ggctcaggcg cgatgttcaa
     1081 gtctccgaga accgccagcc gcggcaggcg cggcagctcc gcttgcagcc acgaagtaag
     1141 ggcctggtac cacctgagct tgtacgcgta cttgtctgtt ccaggagcct cgccgttcgg
     1201 cgcgtagagg cacaggactc gaatgtcgcc gaccgaggcc gcaatgacgc gcttggggtc
     1261 gtcggaaaag tgcggaatac cgtgctggac gtcggtgagc gcggtgcgcg cgaggatcgc
     1321 gacgccgttg tacgccttct gcccgcagta caccgcgtgg tagcccgcgg cctggacctc
     1381 ggcgagcggg aagttatggt cctcggtctt cgtctcctgg aggcacatca cgtccggctc
     1441 gcgcgaacgc agccagtcga gcaggtgagg caggcggatg cggagggagt tgacgttcca
     1501 ggtggcgatg cgcacctacg ccgcgatcat cccattgtgg cgcaggagcg ccgcgacgtt
     1561 aggctcgcgg ccgcggaatg cgcggaacga ttcggccgcc gggcggctgc cgccgacggc
     1621 gagaatctcg tcgcgaaagc gcgcgccggt agcagggtca agaataatgc ccttggctgc
     1681 gccggtttct tcgaatgcac tgtatgcatc agcggaaagc acctcggccc acttgtagct
     1741 gtagtaaccg gccgcgtagc cgccgccgaa gatgtggccg aagctgttcg gaaagcggtt
     1801 gtacgccggc ggaaagagca ccgcgacctg cctgcgcacc tcttgaagca ggtcgagcgc
     1861 gctgcggccg cttgccgggt cgaaatccga atgcaaccgc atgtcgaaca ccgcgaattc
     1921 gatctggcgc agcatcgcga gcccgctctg gaaattcttc gcagccagca ttttttcgaa
     1981 taattgcctt ggtaaaggct gacctgtatc gacatgccgg gtcatgccgc acagcacgtc
     2041 ccactcccag caaaagtttt ccatgaactg gctcggcagc tcgaccgcgt cccattccac
     2101 gccgctgatg cccgagacgc cgagatcctc gacctgggtg aggaggtggt gcaacccgtg
     2161 cccgaactcg tggaaaagcg tgagcacctc gtcgtgcgta aagagcgccg gcttgccgcc
     2221 gaccggccgc gagaagttgc agttcaggta cgccaccggg atctggatgc gttcggccct
     2281 gcgccggcgc gagatcgcgt cgtccatcca cgcgccgccg cgcttcgtct cgcgagcgta
     2341 gagatccagg tagaactggc cgatcgcggt gccgttcgga tcgcgcactt cgaagaagcg
     2401 cacgtccggg tgccacaccg gcgcctggga ttcgacaatc ttcacgccgt agagggtttc
     2461 ggcgacgcgg aagaggccac gctcgacgat gtcctcgggg aagtactgtt tcacttcctg
     2521 gtcggagaac gagtagcgct tgagtcgcag cttctccgac gcataggcga catcccaagc
     2581 ctgcaggtcg ggaatcccaa gctcggttct cgagaactct cttagttcta agacatcgcg
     2641 ttcagcgaac gggcgtgcgc ggcgcgcgag atcgtcgagg aaggcaagca cctggctcgc
     2701 cgactcggcc atcttcggca cgagcgacac ctccgcaaaa tcgcaataac cgagaagctg
     2761 cgcgagctcc cggcgtagcg gcacgatttt cgcgataagc ggcgcgttat cccactgcgg
     2821 gttgccgaat tccgcggcgc gcgtgctgct cgcgtagtac atcttttcgc gcagcaggcg
     2881 ctcgtcggcg tactgcatca ccggcaggta cgagggcatg tggagcgtga acttccagcc
     2941 gtccttgccg tccttgtgcg cagcttcgcg cgcggcctga aggacatcgt cgggaatgcc
     3001 cgaagtctcg tccttggtca cgtaaatcga aaaagcgttg gtcgcgtcca gcaggttctc
     3061 ggagaacttc gccgacaggc gcgcgagctc ctcctggatc tcggcgtagc gcttcttctt
     3121 gtccggcggc agctcggccc cacccaggcg gaaatcgcgc agggcgtttt cgacaatttt
     3181 tttcctcggc gagctcagcg tggaaaactc gttcgaatac ctgagcgcct tgtatttctc
     3241 gaagagctgc aggttctggc cgagctcggt ccagtaactg gtgaccttcg gcagattggc
     3301 gttgtacacc tcgcgcagct ccgggctgtc catcaccgcg tgcaaatgcg cgacctggcc
     3361 ccaagcgcgg ccgaggcgct cgttcgcgtc ctcgagcggc gcaacgaaag cgtcccagct
     3421 gggagcggat ttctccgccg ctttcaccgt cgagcgggcg tcggagagca gaagctcgat
     3481 cgcgggggtg acatgcgcag gcttgatctc cgcgaagcgc ggcaggccgg aaaagtcaag
     3541 aagcggattg ttctcttgcg gcagcacgca ggcagttctc caggagcatt gcgatcgtca
     3601 tcgggccgac gccgccgggc accggcgtga tccagcccgc gatctccttc acggcgtcga
     3661 agtccacatc gcctgcgagc ttgccgtcgg gtaggcggtt cacgccgacg tcgatcacgc
     3721 aggctccggg tttgaccatg tccgcgccaa cgagtttcgc ccgcccgagc gcggcgacca
     3781 gaatatcggc ttgccgcgta atggtttcaa ttcttggagt tttcgaatgg cagatggtga
     3841 cgctcgcgtc cttttgcaag agcagcagcg cgacggg
//
LOCUS       DASTPD010000153         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_695926, whole genome shotgun sequence.
ACCESSION   DASTPD010000153 DASTPD010000000
VERSION     DASTPD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695926"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..368
                     /gene="rplQ"
                     /locus_tag="VFR57_05350"
     CDS             3..368
                     /gene="rplQ"
                     /locus_tag="VFR57_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0258827.1"
                     /translation="MLRHEEVRTTLPKAKELRRVVEPMITLGKKPSLANRRLAFDRLR
                     DRGIVEKLFDDLGPRYAKRNGGYLRILKIGFRNGDNAPMALVTLMDRPEESAQPKPEE
                     KKKKGEQKTTDETKAAKAA"
     gene            369..1106
                     /locus_tag="VFR57_05355"
     CDS             369..1106
                     /locus_tag="VFR57_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU0258828.1"
                     /translation="MFGSLIVLFTDFGSRDLYVGQLKAVLAARAPRVPVIDALHDAAD
                     FAIRPAAHLLFSVASEYPIGSVFLAVVDPGVGGRREAIVVQADGRRFVGPDNGLLSIL
                     WARSRSRKCWRIAWRPKRLTSSFHGRDLFAPVAAALATKRIPKTWLTRKTQPEVRLEG
                     EDLAQIIYIDHYGNAVTGLREFRTAWRLRAGGRTLTYARTFEAARGAFWYENSMGLVE
                     IAAPRRSAAKLLRLSIGSRVAWQRSTR"
     gene            1142..1909
                     /locus_tag="VFR57_05360"
     CDS             1142..1909
                     /locus_tag="VFR57_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258829.1"
                     /translation="MAGRLKDKVAIVTGAGSRGPGLGNGKATAILFAREGANVLCVDN
                     DITRAKETVKTITGEGNHARAFAADVTRASDCAAMVQEAVSSWGGLDILHNNVGIESR
                     LDLMETTEQEWDRIMAVDLKTMFLATQAAVPAMEKRGRGAITCISSIAAMVGHGRTAY
                     AAAKAGVIGLVTSLAVQLGPKGIRVNGIAPGQVWTPMVERLGAEARERRKKASPLGTE
                     GTGWDVGWGAVYLASDEARWVTGHVLVIDAGLTATTR"
     gene            complement(1919..>2272)
                     /locus_tag="VFR57_05365"
     CDS             complement(1919..>2272)
                     /locus_tag="VFR57_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEU0258830.1"
                     /translation="EMGGSEVEAFLSHLATEGRVSPSTQNQALAALLFLYREVLEKEL
                     PWMDGMVRAKRPARVPVVLTENEVRALLAQLDGTLWLASSLLYATGMRLLEGLRLRVK
                     RSPLDRLDQAPERPE"
BASE COUNT          418 a          763 c          743 g          348 t
ORIGIN      
        1 cgctgctgcg gcacgaggag gtccgcacca cgctgccgaa agccaaggag ctgcgacgcg
       61 tggtcgagcc gatgatcacg ctgggcaaga agccctcgct cgccaaccgt cgcctcgcct
      121 tcgatcgcct gcgcgaccgc ggcatcgtcg agaagctctt tgacgacctc ggtccgcgct
      181 acgccaagcg caacggcggc tacctccgca tcctgaagat tggcttccgc aacggcgaca
      241 acgcgcccat ggcgctcgtc acgctgatgg accgccccga ggaatcagcg cagcccaagc
      301 ccgaggaaaa aaagaagaaa ggcgagcaga agacgaccga cgagaccaaa gcggccaaag
      361 ccgcctgatt gttcggtagc ctgattgtcc tatttactga tttcggttcc cgcgacctct
      421 acgtaggtca actcaaggcg gtccttgccg cgcgcgcgcc gcgcgtgccg gtcatcgacg
      481 cgctgcacga cgctgcggat ttcgccatca ggccggcagc tcacctgctt ttctcagtcg
      541 cttccgaata tcctatcggc agcgtatttc ttgccgtggt ggaccccggc gtcggcggaa
      601 ggcgcgaagc catagtggtg caagccgacg gccggcgctt cgtcggcccg gacaatgggc
      661 tgctttccat cctgtgggct cgcagccggt cccgaaagtg ctggcggatc gcctggcgcc
      721 caaagcggct gacgtcttct tttcacggac gcgacctgtt cgcgccggtt gccgccgcgc
      781 tcgcaaccaa gcgcattcca aaaacctggc tgacaagaaa aacgcaaccc gaggtgcggc
      841 tggaaggaga ggatctcgcc cagatcatct atatcgacca ctatggcaac gccgtcaccg
      901 gcctgagaga attcagaaca gcttggcgcc tgcgcgcggg cggcaggacg ttaacgtacg
      961 cgcgcacgtt cgaggccgcg cgcggtgcgt tctggtatga gaacagcatg ggcctcgtcg
     1021 aaatagcggc gccgcgccgc agcgccgcca agctgctccg gctctccatc ggaagccgcg
     1081 tcgcatggca gcgatctacc cggtagggta cggagcatgc tcgcaatccc caggaggagc
     1141 tatggcggga cgtctgaagg acaaggtggc gatcgtcacc ggagccggct cacgagggcc
     1201 ggggctcggc aacggaaaag cgaccgcgat tcttttcgcg cgcgaaggcg ccaacgttct
     1261 ctgtgttgat aacgacatca cgcgcgcgaa agaaaccgtc aagacaatta ctggtgaagg
     1321 caaccacgcc cgggccttcg ctgccgacgt cacgcgcgca tccgactgtg cagcgatggt
     1381 gcaggaggcg gtgtcgagct ggggtgggct cgatatcttg cacaacaacg tcggcatcga
     1441 atcgcgcctc gatctgatgg agaccaccga gcaggaatgg gaccggatca tggcggtcga
     1501 tctcaagacg atgttcctcg cgacgcaggc cgcggtgccg gcgatggaga agcgcggccg
     1561 cggcgccatc acgtgcattt cttcgatcgc ggcgatggtg ggccacggcc gcaccgccta
     1621 cgccgcggcc aaggccggcg tcatcggcct ggtcacaagc ctcgcggtgc agctcggccc
     1681 caagggcatc cgcgtgaacg gcatcgcgcc cgggcaggtc tggacgccga tggtcgagcg
     1741 cctcggcgcc gaggcgcgcg agcgccgcaa gaaagcttcg ccgctcggca ccgagggcac
     1801 gggctgggac gtgggctggg gcgcggtgta cctcgcgagc gacgaggcgc gctgggtcac
     1861 cggccacgtc ctcgtcatcg acgccggcct caccgccacc acgcgctgag ggctggcttc
     1921 actcgggccg ctcgggcgct tgatccaacc ggtcgagcgg gctcctcttg acgcgcagcc
     1981 gaagcccttc gagcagccgc atgccggtcg cgtagagcag gctcgaagcg agccagagcg
     2041 tgccgtcgag ctgggcgagc agcgcccgca cttcgttctc cgtgagcacc accggaacgc
     2101 gcgccgggcg cttcgcgcgc accatgccgt ccatccacgg aagctccttt tccaggactt
     2161 cgcgataaag aaaaaggagg gccgccagcg cctggttctg cgtcgaaggc gagacccgcc
     2221 cctcggtcgc gagatgcgac aggaacgcct cgacctcgga cccgcccatc tc
//
LOCUS       DASTPD010000154         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1103581, whole genome shotgun sequence.
ACCESSION   DASTPD010000154 DASTPD010000000
VERSION     DASTPD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103581"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFR57_05370"
     CDS             <1..864
                     /locus_tag="VFR57_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258831.1"
                     /translation="IGISGWTGFAPLTLALQAGIFKKNGVEATIKKIPQQSRHLAIAS
                     GDIQCAATTVETWLVWNANGVLTRQIFQLDKSFGADGMATRANIAKIADLKGKTIGSQ
                     APGTSPYFVMAWMLKKNGLTPKDVTVVSIDPGPAAQAFLAGQYDAVMTYEPYLSSIRA
                     KPDAGKIIATTLDYPVVMDTFGCTPKYLAEHGRAARAMTQSYFQALEMIAKEPQKAYE
                     IMGADVKQSGEQFANSAKYLRWQDRGANQKFFAGELQAFLKEAGQILLEIGIIKSMPD
                     VNSTIDTSYIK"
     gene            866..1639
                     /locus_tag="VFR57_05375"
     CDS             866..1639
                     /locus_tag="VFR57_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258832.1"
                     /translation="MTPLKPVGPGARFALGAAFFVLFFAAWALATFGGYVSPTFLADP
                     ATMMREGWNLLVQHGFIKDILITIWRVFGGFLLAAAIGIPLGMAMGAYKPIEAFFEPF
                     ISFSRYLPASAFIPLLILWAGTGEAQKLAVIFVGSFFQIVLMVAVSVGNTRRDLVEAA
                     YTLGASDRAIVTRVLLPAAAPEIAEISRLVLGWAWTYVIVAELIGASSGIGHMIMDSQ
                     ALLNTGQIIFGIVVIGVIGLVSDYLFKALNRRLFPWSLA"
     gene            1636..2400
                     /locus_tag="VFR57_05380"
     CDS             1636..2400
                     /locus_tag="VFR57_05380"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258833.1"
                     /translation="MSKLAIEGVSKNFAQTKALERIDLAIADNDFLCILGPSGCGKST
                     LLRIVAGLEAPSTGRVLLDGQAVERPGPDRGMVFQSYTLFPWLTVRQNVLFGTRSTAE
                     KADSLIRRVGLKGFENHHPKQLSGGMQQRTALARALANDPKILLLDEPFGALDNQTRA
                     LMQELLLGIWEADRKTVLFVTHDIEEAIFMANRVAVMSARPGRIKSIVPIALPHPRHY
                     TMKTTPEFSRYKAELTEEIRVESIRTMEMAVGGLLG"
BASE COUNT          473 a          833 c          792 g          450 t
ORIGIN      
        1 atcggcatca gcggctggac cggctttgcg ccgctcacgc tcgcgctgca ggcaggcatc
       61 ttcaagaaga acggcgtcga ggcgaccatc aagaagatcc cgcagcagag ccgtcatctc
      121 gccatcgcct cgggcgacat ccagtgcgcg gcgaccacgg tcgagacctg gctcgtctgg
      181 aatgcgaacg gcgtgttgac caggcagatc ttccagctcg acaagagctt cggcgccgac
      241 ggcatggcga cgcgcgcgaa catcgcaaaa atcgccgatc taaagggcaa gacgatcggc
      301 tcgcaggcgc cggggacctc gccgtatttc gtcatggcat ggatgctgaa gaagaacggg
      361 cttacgccga aggatgtcac cgtcgtgtcg atcgatccgg ggccggcggc gcaggcgttc
      421 ctcgccgggc agtacgacgc ggtcatgacc tacgagccgt atctctcctc catccgcgcg
      481 aagcctgacg caggcaagat catcgccacc acgctcgact atccggtggt gatggacacg
      541 ttcggctgca cgccgaaata tctcgccgag cacggccgcg ccgcgcgcgc gatgacgcaa
      601 agctacttcc aggcgctcga gatgatcgcg aaagagccgc agaaggccta cgagatcatg
      661 ggcgccgacg tgaagcagag cggggagcag ttcgcaaact cggccaagta cctgcgctgg
      721 caggaccgcg gcgcgaacca gaagttcttc gccggcgagc tgcaggcctt tctcaaggaa
      781 gcggggcaga tcctcctcga gatcggcatc atcaagtcga tgcccgacgt gaacagcact
      841 atcgacacga gctacatcaa gtagcttgac gcctctcaag ccggtcgggc cgggcgcgcg
      901 gttcgcgctc ggcgcggcgt ttttcgtgct tttcttcgcc gcgtgggcgc tcgccacctt
      961 cggcggctac gtctcgccga ccttcctcgc cgaccccgcc accatgatgc gcgaaggctg
     1021 gaacctgctc gttcagcacg gcttcatcaa agacatcctg atcaccatct ggcgcgtatt
     1081 cggcggcttt ctcctcgccg cggcgatcgg catcccgctc gggatggcga tgggcgcgta
     1141 caagccgatc gaggccttct tcgagccgtt catttcgttc tcgcgctacc tgccggcgtc
     1201 tgcattcatt ccgttgctca tcctctgggc ggggaccggc gaggcgcaga agctcgccgt
     1261 cattttcgtc ggctcgttct tccagatcgt gctcatggtg gcggtgagcg tggggaacac
     1321 gcggcgcgat ctggtggaag cggcctatac gctcggcgcg agcgaccggg caatcgtgac
     1381 gcgcgtcctg ctaccggccg cggcgccgga gatcgccgag atctcgcgcc tcgtgctcgg
     1441 gtgggcctgg acctacgtca tcgtcgcgga gctgatcggc gcctcctcgg gcatcggcca
     1501 catgatcatg gacagccagg cgctcctcaa taccggccag atcatcttcg gcatcgtggt
     1561 gatcggcgtg atcggcctgg tttcggacta ccttttcaag gcgctcaacc ggcggctctt
     1621 tccctggagc ctcgcgtgag caagctcgcg atcgaaggag tatcgaagaa ttttgctcaa
     1681 accaaggcat tggaaagaat cgatctcgcg atcgcggaca acgacttcct ctgcatcctc
     1741 ggcccgtcgg gctgcggcaa gtccacgctc ttacgcatcg tcgccgggct ggaagcgccg
     1801 agcaccgggc gcgtgctgct cgacggacag gcggtcgagc gccccggccc cgaccgcggc
     1861 atggtgttcc agtcctacac gcttttcccc tggctcaccg tcagacagaa cgtcctgttt
     1921 ggcacccgat cgacggccga aaaggccgat tcgctgattc gcagggtcgg cctaaaggga
     1981 ttcgagaacc accacccgaa acaactctcc ggcggcatgc agcagcgcac ggcgctggcg
     2041 cgcgcgctcg ccaacgaccc gaagatcctg cttctggacg agcctttcgg cgcgctcgac
     2101 aaccagacac gggcgctcat gcaggagctg ctgctcggca tctgggaggc cgaccgcaag
     2161 accgtgctgt tcgtcaccca cgacatcgaa gaagcgatct tcatggcgaa ccgcgtcgcg
     2221 gtaatgtcgg cgcggcccgg gcgcatcaag tccatcgtgc cgatcgcgct gccgcacccg
     2281 cggcactaca ctatgaaaac cacgccggag ttctcgcgct acaaggcaga gctcactgaa
     2341 gagatccgcg tcgagtcaat ccggacaatg gaaatggcgg tcggggggtt gctcgggtag
     2401 gtttctgaaa ggccaacgac cgtgaaaaag cgctcctcgc gccgcacttt gaagtctgac
     2461 ctgaccaagg tcgatgccca cgtaattcgc tcgcatgagt acaaggaact gcccgaactt
     2521 gcggagcgag ccttgtcgcg tgctgtcg
//
LOCUS       DASTPD010000155         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_287548, whole genome shotgun sequence.
ACCESSION   DASTPD010000155 DASTPD010000000
VERSION     DASTPD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287548"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFR57_05385"
     CDS             complement(<1..213)
                     /locus_tag="VFR57_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0258834.1"
                     /translation="MRSLIALLLFWPFAALGGAQIYEPLADNVRQRLSSLVADRAPAS
                     MHFREAADGQRWLAEMGQRLERRIPDR"
     gene            complement(210..1901)
                     /locus_tag="VFR57_05390"
     CDS             complement(210..1901)
                     /locus_tag="VFR57_05390"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0258835.1"
                     /translation="MKTSKFFLSTQKESPAEAELVSHRLMLRAGIIRRVAAGIYTWLP
                     LGMRVAQKVAGIIREEMNRAGALELAMPVVQPGELWQESGRWQAYGPELLRFRDRHER
                     EFVIQPTSEEVVTELARAELKSYRRLPVHFYQIQTKFRDERRPRFGIMRAREFIMKDG
                     YSFHADHADLEREYRNMHETYTRIFTRLGLRFRAVAADTGAIGGTGSHEFHVLADSGE
                     DAIAFSPDSDYAANVELAEAITAEKRKAPTQAMQKVSTPGKTTCEQVARLLKLGLQQT
                     VKAVAVMHEDDFYLLLIRGDHALNEIKTQKLVGAFRFATDGEIQQFLGCKAGYIGPVG
                     ASAKVIADRAVSAMSDFVCGANEEGFHLSGVNWGRDLPEPRVADIRNVVAGDPSPDGK
                     GRLEIARGIEVGHIFQLRTKYSESMKATFLAQDGTARAFEMGCYGIGVTRIVAAAIEQ
                     NHDARGIVFPAPIAPFQACLVPIGYHKSPQVREAAERLYGEMLQAGIDVLMEDREERP
                     GVLFADMELVGIPHRVVLSERGLAAGNAEYKGRRDDKPQDLKLDATVDFLRQRLV"
     gene            2028..2531
                     /locus_tag="VFR57_05395"
                     /pseudo
     CDS             2028..2531
                     /locus_tag="VFR57_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205795.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
BASE COUNT          440 a          890 c          874 g          474 t
ORIGIN      
        1 gcgatcgggg atgcgccgct cgaggcgctg ccccatctcg gcgagccagc gctgcccatc
       61 ggcggcctcg cggaaatgca tcgacgccgg cgcgcggtcc gcgaccaggg acgagagccg
      121 ctggcgcacg ttgtcggcga gcggctcata gatctgcgcg ccgccgagcg cggcgaacgg
      181 ccagaagaga agaagagcga tgagcgatct cacaccagcc gttgcctgag gaaatccact
      241 gttgcatcga gcttcaggtc ctgcggcttg tcgtcacgcc ggcccttgta ttcggcgttg
      301 ccggcggcga ggccgcgctc ggagagcacc acgcgatgcg gaatgccgac gagttccatg
      361 tcggcgaaca gcaccccggg acgctcttcc cgatcctcca tgaggacatc gatcccggcc
      421 tgcagcatct cgccgtaaag ccgctcggcg gcctcgcgca cctgcgggct cttgtggtag
      481 ccgatcggca ccaggcacgc ctggaacggc gcgatcggcg cgggaaatac tatcccgcgc
      541 gcatcgtgat tttgctcgat cgccgcggcc acgatgcgcg tcacgcctat gccgtagcag
      601 cccatctcga acgctctcgc ggttccgtcc tgtgccagga aggtcgcctt catgctctcg
      661 gaatacttgg tgcgcagctg gaaaatatgc ccgacctcga tgccgcgcgc gatctcgagc
      721 ctccccttgc cgtccgggct gggatcgccc gcgaccacgt tgcggatgtc ggccacccgg
      781 ggctcgggca ggtcgcgacc ccagttgaca ccgctcaagt ggaacccttc ctcgttggcc
      841 ccgcatacga aatcgctcat cgccgagacc gcacggtcgg cgatcacctt tgcgctggct
      901 ccgaccgggc cgatgtagcc cgctttgcag ccaaggaatt gctgaatctc cccatcggtg
      961 gcgaagcgga aggcgccgac gagcttttgc gtcttgatct cgttcagcgc gtgatcgccg
     1021 cggataagga gcagatagaa atcgtcctcg tgcatgacgg cgactgcttt taccgtctgt
     1081 tgcagtccga gcttcagcag ccgcgcaacc tgctcgcagg tcgtcttgcc cggcgtggaa
     1141 accttttgca ttgcctgagt cggcgctttg cgcttttcgg cggtgattgc ttcggccaat
     1201 tccacgtttg ccgcatagtc ggaatcgggc gagaaggcga tcgcgtcctc gcccgagtcg
     1261 gcgaggacat ggaactcgtg cgaaccggtg ccgccgatcg cgccggtatc ggctgctacg
     1321 gcgcggaaac gcaggccgag tcgggtgaag atccgggtat aggtctcgtg catgttgcgg
     1381 tactcgcgct cgagatccgc gtggtcggca tggaatgagt atccgtcctt catgatgaat
     1441 tcccgcgcgc gcatgatgcc aaagcgcggc cggcgctcgt cgcgaaactt ggtctggatc
     1501 tggtaaaaat gcacaggcag gcgccggtag ctcttcagct cggcgcgggc gagctcggtg
     1561 accacttcct ccgaggtcgg ctggatcacg aactcacgct cgtggcggtc gcgaaagcgc
     1621 aaaagttccg gcccataggc ctgccagcgc cccgactctt gccacagctc cccgggctgc
     1681 accaccggca tggcgagctc gagggcgccg gcgcgattca tttcctcgcg gatgatcccc
     1741 gccacctttt gggccacgcg catgccgagc gggagccagg tgtagattcc ggcggccacg
     1801 cgccggatga tccccgcgcg cagcatgagg cggtggctta cgagctcggc ctcagccggg
     1861 ctttccttct gcgtcgagag aaagaacttc gaggttttca cttcgaggtt ttcatcgcgc
     1921 ccgcaccggc gttgtctgcc gtgcaccggc gcttcctgcc ggcgttgtct ttggcggtgg
     1981 gctgtggaaa aatcgtccga ggattctaac tgaaggcggt ccggcccatg ctcgacaagg
     2041 aaggctaccg tccgaacgtc ggcattatcc ttgccaacca gaggaatgag gtgttctggg
     2101 gcaagcgcgt gaatcagcac gcctggcagt ttccgcaagg cggcatcaag ccgggcgaga
     2161 cgcccctgca ggccatgtac cgcgagctcg aggaggagat cggcctgctg cgcagccacg
     2221 tgcgcatcct cggccgcacg cgcgaatggc tgcgctatga agtgcccgag cgctgggcgc
     2281 ggcgcagccg cgagggcggc ggggccaagg ccggcaccgc ctaccgcggc cagaagcaga
     2341 tctggttcct cctgcgcatg gtgggccgcg actgcgacgt taagcttcgc gcaagcgggc
     2401 atcccgaatt cgacgcgtgg cgctggcatg attactgggt gccgctcgac accgtgatcg
     2461 acttcaagcg cgacgtctac cgccaggcgc tgctcgagct gcatcgctac ctgcaagccg
     2521 atcgccgcgc gcgtccgcgc cggctggaga ccgctcacct ctaggaagcc cggttatgaa
     2581 tcgcgcctac cagccgctgc ccagccgcga gctgggcgca aacgccggct ttcgccggcc
     2641 cctgcagccg caatcgccgc gcgtgaacgc ggagtcgg
//
LOCUS       DASTPD010000156         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_287837, whole genome shotgun sequence.
ACCESSION   DASTPD010000156 DASTPD010000000
VERSION     DASTPD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287837"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VFR57_05400"
     CDS             <1..1168
                     /locus_tag="VFR57_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0258836.1"
                     /translation="ETATTYRAWERLQELVDEADRNFTQRERERADVEEQVNDLKKVS
                     PQEGEWEKVSAEHTRLQHGSSLLGGAQSALETLAEAEGACLSQLAAVANRLKTLSQHD
                     HQLNGVVEMLESAEAQASEAVRTLRQYASRVDLDPEALREVDARIETLHAAARKYRVR
                     PEALHEVLQDLEKRRGELELAVNPEALRREVAAAKTSFDSLCRKLSLKRRAGAEALGK
                     AVTAAMQQLAMAGGRFSVSLRKLDEPGASGAEAVEFEVSSHPSLPLRPLAKVVSGGEL
                     SRISLAIQLVAAKASPVASLVFDEIDSGIGGAVAETIGKSLRRLGKQRQVLCVTHLPQ
                     VAASGNDQWSVSRAGVKVKVQRLDRTARIVELARMLGGAESTARKHAAELLDAT"
     gene            complement(1242..1667)
                     /gene="fur"
                     /locus_tag="VFR57_05405"
     CDS             complement(1242..1667)
                     /gene="fur"
                     /locus_tag="VFR57_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEU0258837.1"
                     /translation="MSTVQSLKDSGLKATLPRRKILELFESSKVRHLSAEDVYKALLS
                     EGLDVGLATVYRVLTQFEQAGLLARQHFETGKAVFELNQGGHHDHLLCLQCGRVEEFY
                     DPDIEKRQTEIARSRGFALHGHSLALYADCTKKDCPYHP"
     gene            1687..>2015
                     /locus_tag="VFR57_05410"
     CDS             1687..>2015
                     /locus_tag="VFR57_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEU0258838.1"
                     /translation="MPRRLFPLFVAALCLGILGACSSAPRIPGITPYRIDIQQGNYIS
                     SEMVAQLKPGMSKEQVRLALGTPLLTDIFHADRWDYVYWYERAGAKPEQRKLTVFFED
                     GKLTRLDG"
BASE COUNT          363 a          633 c          685 g          334 t
ORIGIN      
        1 agaaaccgca actacctatc gagcctggga gcggctccag gagctcgttg acgaggcgga
       61 taggaacttc acgcagcgtg agcgcgagcg cgccgacgtc gaagaacaag tgaatgacct
      121 aaagaaagtt tcgccccagg aaggcgaatg ggaaaaggtc tcggccgagc atacgcgcct
      181 gcagcacggc tccagcctgc tgggcggcgc gcagtcggcg ctggagacac ttgccgaagc
      241 ggaaggcgca tgcctcagcc agctcgccgc ggtggcgaac cggctgaaaa ccttatcgca
      301 gcatgaccat cagttgaacg gcgtggtcga gatgctcgaa tccgccgagg cgcaggcgag
      361 cgaagcagtg cgcacgttgc gtcaatacgc ctcgcgcgtg gacctcgatc cggaggcgct
      421 gcgcgaagtc gacgcgcgga tcgagacgct gcacgccgca gcgcggaagt accgggtgcg
      481 gcccgaagcg ctgcatgagg tgctgcaaga cctggaaaag cggcgtggcg agctcgagct
      541 tgcagtgaac cccgaagcgc tgcgccggga ggttgccgcg gcgaagacga gcttcgattc
      601 cctttgcagg aaattgagtt taaagcgccg cgccggcgcc gaggcgctcg gcaaggcggt
      661 cacggcggcg atgcagcagc tcgccatggc cggcgggcgc ttttcggtct ccctgagaaa
      721 gctggacgag cctggtgcgt ccggcgccga ggcagtggag ttcgaagtat cgtcccaccc
      781 gagcctgccg ctgcggccgc tggccaaggt ggtctccggc ggggagcttt ctcgtatcag
      841 cctcgcgatc caactcgtcg cggcgaaagc ttcgccggtc gcgtcgctcg tcttcgacga
      901 gatcgactcc gggatcggcg gtgcggtggc cgagactatc ggaaagtcgc tgcgtcggct
      961 cggcaaacag cgccaggtgc tttgcgtcac tcacttgccc caggttgcgg cgagcggcaa
     1021 cgaccaatgg tccgtgagcc gcgcaggggt gaaggtgaag gttcagcgcc tagaccggac
     1081 ggcgcgcatt gtggagcttg cacgcatgct gggaggcgcg gaaagcaccg cgcgcaagca
     1141 cgcggccgag ctcctcgacg cgacctgaat ggccagtaga tcggggtcag gccccaaccg
     1201 aaaaatcggg gtctgaccag ctctgacccc gatttaccgc ttcatgggtg gtacgggcag
     1261 tccttcttgg tgcagtcggc gtagagcgcg agcgaatgcc catgcagcgc gaagccgcgc
     1321 gagcgggcga tctcggtctg gcgcttctcg atgtcggggt cgtagaactc ctcgacccgg
     1381 ccgcactgca ggcagagcag gtggtcgtgg tggccgccct ggttgagctc gaacaccgcc
     1441 ttgccggtct cgaagtgctg gcgcgcgagc aggccggctt gctcgaactg cgtcagcacg
     1501 cgatacacgg tggcgaggcc gacgtcgagg ccctcggaga gaagcgcctt atagacgtcc
     1561 tcggcggaga gatggcgtac cttgctcgat tcgaagagct cgagaatctt gcgccgcggc
     1621 agcgtcgcct tgaggccgct gtccttcagg ctttgcaccg tgctcatcgg ctatcattat
     1681 attcggatgc ctcgccgcct tttcccgctc ttcgtcgcgg cgctctgcct cggtattctg
     1741 ggcgcttgct catccgcgcc acgcattccg ggtatcacgc cgtaccgcat cgacatccag
     1801 cagggcaact acatctcctc ggagatggtc gcccagctca agcctggcat gagcaaggag
     1861 caggtgcgcc tcgcgctcgg cacgccgctg ctcaccgaca tctttcacgc cgaccgctgg
     1921 gactacgtct actggtacga gagggccggc gcaaagcccg agcagcgcaa gctcaccgtg
     1981 ttcttcgagg acggcaagct cacgcgtctc gacgg
//
LOCUS       DASTPD010000157         7667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_491956, whole genome shotgun sequence.
ACCESSION   DASTPD010000157 DASTPD010000000
VERSION     DASTPD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491956"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFR57_05415"
     CDS             <1..785
                     /locus_tag="VFR57_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258839.1"
                     /translation="GKWACENGFKTVSAIAADYAFGHEVLGGFQKAFEDCGGRVVQKI
                     WPPLGTKDFGPYIPTIKRDVDAIFTVMVGPMALQFPKQLQEAGIKKPVIGGGTSYDEF
                     VLPSMGDEVLGHVSGLQYSAAIDTPRNREFVRRYREKYGKVPSYYSESNYTTAQMIHE
                     VMKQTKGKWPGPEKFIQMITQLKLDTPRGPVRFDDMRNPIQNIYIKKVEKKKMHGYDK
                     DELWNSVIQTYPEVSQFFTYKKEEFLKQPVYSRDFPPCKHCN"
     gene            821..1507
                     /locus_tag="VFR57_05420"
     CDS             821..1507
                     /locus_tag="VFR57_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258840.1"
                     /translation="MREVTLEVAPGERKAIIGPNGAGKTTLFNLITGVFPPTSGEIIL
                     FGKEVTRWPPHKRTALGMARTFQITSLFPKLTVLDNVLLGLAGVGRSKLVMWRPLSSY
                     EKFQNRARGLLEGAGYWDRRNVEVRNLSHGEQRQIEVLLGLASDPKLLLLDEPAAGLS
                     SGESREMAEFLKRLDPSLAILLIEHDMDVVFDVVSSISVLHFGAVVEQGSAAAIKRSE
                     RVQQIYLGTG"
     gene            1509..2207
                     /locus_tag="VFR57_05425"
     CDS             1509..2207
                     /locus_tag="VFR57_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258841.1"
                     /translation="MAILSVEDIHTYYGDAYVLQGLSLELGEGQIVGLLGRNGVGKTT
                     LVNSVVGFNRPRRGRIVFKGADITGVSSFETVRRGMGLVPQGRRVFASLTVEENLKVA
                     QAGNGGGWTLKRVYELFPRLEERRHQRSKTLSGGEQQMLAIARGLMTDPDCLIMDEPS
                     EGLAPIIIQTVWRAVSKLKEQGLSILLVEQNAALALQLVDYVHVVSKGRVVYSGAPKA
                     LWADDAVKSKYLGI"
     gene            2197..2484
                     /locus_tag="VFR57_05430"
     CDS             2197..2484
                     /locus_tag="VFR57_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0258842.1"
                     /translation="MESEIQHERSGHRGAFVLERSGKRLAEMTYTVAGTRVIIDHTQV
                     DDSLRGEGVGAKLVQAAVEWARAENARLMPLCPYAKSVFDKTPAYADVLAK"
     gene            complement(2486..2692)
                     /locus_tag="VFR57_05435"
     CDS             complement(2486..2692)
                     /locus_tag="VFR57_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258843.1"
                     /translation="MKPTLFNALIVALFASLLPACERVATDREEAAASKGGTAGQTPA
                     RGATGQASEGSANDRNAPARPSSK"
     gene            2733..3134
                     /locus_tag="VFR57_05440"
     CDS             2733..3134
                     /locus_tag="VFR57_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0258844.1"
                     /translation="MISGSCLCRAIRYEVTVPISELRHCHCKDCQKSSGAGGAVNAMI
                     PSSGFRITHGEPRRFTKVADSGRTLHRFFCGDCGSPIYSRREVLPEITSLRIGTLDNA
                     PPMKIATNIWTESARPWAHIDPATQRFPGQP"
     gene            3140..3562
                     /locus_tag="VFR57_05445"
     CDS             3140..3562
                     /locus_tag="VFR57_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0258845.1"
                     /translation="MLTGSCLCRAIRYEVNIPITELRQCHCTDCQKVSGAGGTVNALI
                     ASSAFRITHGEPRRFTKVADSGRTLHRFFCADCGSPIYSQREVTPEMMTLRAGTLDNA
                     PPMKVVAHIWTASARSWAHIDPATQRFPGQPDAPAVKK"
     gene            3591..4067
                     /locus_tag="VFR57_05450"
     CDS             3591..4067
                     /locus_tag="VFR57_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU0258846.1"
                     /translation="MRAVKALPLVLAFLAAGCTSAPNRAPEAPTRSILASAEMADDAA
                     THALKMVGRPYKYGGSTPAGFDCSGLIRYSFGRAGLSLPHGTDKLRGVSNVVKTSELR
                     RGDLLFFHQEGKRYGHAGIYLGNGRFVHAPSSGKSVRTDGLDAPYWKRHLSEVRRL"
     gene            4087..4482
                     /locus_tag="VFR57_05455"
     CDS             4087..4482
                     /locus_tag="VFR57_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0258847.1"
                     /translation="MSIKLNHTIVHSRDQREAAEFLASLFGLPAPQPFGPFLDVDVAN
                     EVTLAFLDADGMEIQTQHYAFLVSDDEFDQIFARIQARGLKYWADPGRTEEGRINHHF
                     GGRGVYFQDPSGHLMEIITRPYGAFPKAR"
     gene            4514..5611
                     /locus_tag="VFR57_05460"
     CDS             4514..5611
                     /locus_tag="VFR57_05460"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEU0258848.1"
                     /translation="MRKLYAALLILLFAVPVQAVTFVYVSNAEDGDIGQYMMRPDGTL
                     QPVTRFKAGPNVMPMSVSPDKRYLVAAGRAKPFTAYTYAIDRGTGALKLVGTGPLAES
                     FPYITHDRTGRWLLSASYGGHLVSVNPLKPDGTVGDPHQVIPTARNAHAIISDRTNRH
                     VFVPHLGTDQVFQFLFDEKSGRLSASTPPVVQMKAGMGPRHLVMSPDNRFVYLLNELT
                     ATVTTLANENGLLREISSVSALPPGSTLRPGMPRPQPDRDVSNDVWASDIHVAPSGRF
                     VYAAERTSSSIAAFSADPANGKLNYLGSTSTEKQPRGFRIDPTGRFMIVSGEKSDTIS
                     SYRIESSGLLLHLGKYSTGKGSNWVEIVSYD"
     gene            complement(5642..5872)
                     /locus_tag="VFR57_05465"
     CDS             complement(5642..5872)
                     /locus_tag="VFR57_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258849.1"
                     /translation="MLQDIHRRALQRAATILGGNEKLRVHLGASESDFESWPSRRELP
                     REIFLRLVDIITEEEVRTVQRYRAPRRVANSQ"
     gene            5950..6729
                     /locus_tag="VFR57_05470"
     CDS             5950..6729
                     /locus_tag="VFR57_05470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0258850.1"
                     /translation="MSVARVVFVSLLSVFTAAACAQQPATSTYEPTVGQEGKDVVWVP
                     TPQALVDKMLDMAKVTANDFVMDLGSGDGRTVITAAKRGARAVGVEYNPQMVELSKRN
                     AQKEGVTDRAEFMKADLFETDFSKATVITMFLLPDINLKLRPKLLQLKPGTRVVSNSF
                     TMGDWQPDETAELNSAQGCTNSWCTALLWIIPARVAGTYKTAQGEVTFKQQYQMLSGT
                     MRTAERTVPIEGKVRGEDITFKAGGKEYRGRLNGKQLELKS"
     gene            complement(6726..>7667)
                     /locus_tag="VFR57_05475"
     CDS             complement(6726..>7667)
                     /locus_tag="VFR57_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0258851.1"
                     /translation="GFDIISGDARFGFGIPEMMDGVDFLPVAIGLFGLGEVLYGAEQK
                     GATQVKTSLRLRDVLPSVQDFVRTKWSIARGTLMGFLVGVLPGAGPTVAAFMAYTVEK
                     RVSDEPEKFGKGEIKGVAGPESANNAAATAAMVPMLTLGIPGSATTAIMLGGLMMWGL
                     RPGPMLFEKNPQFVWGLIASQYIANVLLLIMSTLFIPLFVRAVRIPYSILMPLIVVFC
                     VTGAYSLKNNVWDVGMMLVFGVVGYFMKKLGYSPAALVLALVLGPLAERALRQALIIS
                     DAGIFILFMRPISATLTVLALVAIAVPVCRALMTRAR"
BASE COUNT         1466 a         2493 c         2436 g         1272 t
ORIGIN      
        1 tcggcaaatg ggcgtgcgag aacggcttca agaccgtgtc ggcgatcgcc gccgactacg
       61 ccttcggcca cgaggtgctc ggcggcttcc agaaggcatt cgaggactgc ggcgggcgcg
      121 tggtgcagaa gatctggccg cccctcggca cgaaggactt cggcccgtac atcccgacca
      181 tcaagcgcga tgtcgacgcg attttcaccg tcatggtcgg ccccatggcg ctacagttcc
      241 cgaagcagct gcaggaggcc ggcatcaaga agccggtgat cggcggcggc accagctacg
      301 acgagttcgt cctgccgtcc atgggcgacg aggtgctcgg gcatgtctcc gggctgcaat
      361 acagcgcggc catcgacacg ccgaggaacc gcgagttcgt gcgccgctac cgcgagaagt
      421 acggcaaggt gccttcctac tactcggagt cgaactacac cacggcacag atgatccacg
      481 aggtaatgaa gcagacgaag ggcaagtggc ccgggccgga gaagttcatc cagatgatca
      541 cgcagctcaa gctcgacacg ccgcgcggcc cggtgcgctt cgacgacatg cgaaatccga
      601 tccagaacat ctacatcaag aaagtcgaga agaagaagat gcacggctac gacaaggacg
      661 agctctggaa ctcggtgatc cagacctacc ccgaggtgag ccagttcttc acctacaaga
      721 aggaagagtt cctgaagcag ccggtctaca gccgcgactt cccgccctgc aagcactgca
      781 actaaaaggg ctgtcgaaaa acttcggggg cctcgccgcg gtgcgcgagg tcaccctcga
      841 agtcgcgccg ggcgagcgca aggccatcat cggcccgaac ggcgccggca agaccacgct
      901 cttcaacctc atcaccggcg tttttccgcc gacctccggc gagatcatcc tctttggcaa
      961 ggaggtcacc cgctggccgc cgcacaagcg caccgcgctc ggaatggcgc gcacctttca
     1021 gattacgagc ctctttccga agctcacggt gctcgacaac gtgctgctcg gcctggcggg
     1081 cgtggggcgc agcaagctcg tgatgtggcg accgctctca tcctacgaga aattccagaa
     1141 ccgcgcgcgc gggcttctcg aaggcgcggg ttactgggac cggcgcaacg tcgaggtgcg
     1201 caacctctcg cacggcgagc agcgccagat cgaagtgctg ctcggactcg cgagcgatcc
     1261 gaagctcctt ctcctcgacg aaccggcggc aggcctctcc tccggcgaat cgcgcgagat
     1321 ggccgagttc ctgaagcgcc tcgatccgtc gctcgccatt cttctcatcg agcacgacat
     1381 ggacgtcgtc ttcgacgtgg tgtcgagcat ctcagtcctg cacttcggcg ccgtcgtgga
     1441 gcaaggcagc gccgcagcaa tcaagcgaag cgagcgggtg cagcagatct acctcgggac
     1501 cggctgagat ggcaatcctc tcggtcgagg acatccacac ctactacggc gacgcgtacg
     1561 tcctgcaggg actctcgctc gagctcggcg agggacagat cgtcgggctc ctggggcgaa
     1621 acggcgtcgg caagaccacg ctggtgaact cggtggtcgg cttcaatcgc ccgcggcgcg
     1681 gcaggatcgt gttcaagggc gcggacatca ccggcgtttc ctcctttgag accgtgcgcc
     1741 gcgggatggg actcgtgccg cagggccggc gcgtgtttgc ttcgctcacg gtcgaggaga
     1801 acctcaaggt cgcgcaggcg gggaacgggg gcggctggac gctgaagcgc gtctacgagc
     1861 tattcccgcg gctggaagag cggcgccacc agcgctccaa gacgctctcc ggcggagagc
     1921 agcagatgct cgccatcgcg cgcggcctca tgacggaccc cgattgcctc atcatggacg
     1981 agccttccga gggactggcg ccgatcatca tccagaccgt ctggcgggcg gtgtcgaagc
     2041 tcaaggagca gggtctctcg atcctgctcg tcgagcagaa cgcagcgctc gcgctccagc
     2101 tcgtcgatta cgtgcacgtc gtcagcaagg ggcgcgtggt gtactccggc gcgccgaaag
     2161 cgctgtgggc cgatgacgcg gtaaagtcca agtatcttgg aatctgaaat ccagcatgag
     2221 cgttcgggcc accgcggcgc ctttgtcttg gaacgatctg gaaaacggct ggccgagatg
     2281 acctacacgg tcgccggtac gcgtgtgatc atcgatcaca cgcaagtcga cgactcgttg
     2341 cgcggcgagg gcgtcggggc gaagctcgtg caggcggcgg tcgagtgggc gcgcgcagag
     2401 aatgcgcgcc tgatgccgct ctgtccgtac gcgaagtcgg tgttcgacaa gacgcccgct
     2461 tacgcggacg ttctggcgaa atagcctact tgctcgaagg acgcgcgggc gcgttgcgat
     2521 cgttggccga gccttcgctc gcctggccgg tagcgccccg ggccggcgtc tggccggccg
     2581 tcccgccttt gcttgccgcg gcctcctcgc ggtcggtggc gacacgctcg caagctggca
     2641 acaagctggc gaacaaagct acgatcaatg cgttgaacag cgtcggtttc atcgtcgtct
     2701 cctgaccacg actagcaagg aaaggaccgc ccatgataag cgggagctgc ctctgtcgcg
     2761 cgatccgcta cgaagttacc gtgccgatta gcgagctgcg ccactgccat tgcaaggact
     2821 gccagaagtc gtcgggcgcc ggcggcgcgg tcaacgcaat gatcccgagc tctggcttcc
     2881 gcatcacgca cggcgagccc aggcgcttta caaaagtcgc cgacagcggc cgcacgctgc
     2941 accgcttctt ctgcggcgac tgcggctcgc cgatctacag ccggcgcgag gtgctgccgg
     3001 agatcacgtc gctgcgcatc ggcacgctgg acaacgcgcc gccgatgaaa atcgccacca
     3061 acatctggac cgagagcgcg cggccgtggg cgcacatcga tccggcgacg cagcgctttc
     3121 cggggcaacc gtagaaccca tgctgaccgg aagctgcctg tgccgcgcga tccggtacga
     3181 ggtgaacata ccgatcaccg agttgcgcca gtgccactgc acggactgcc agaaggtctc
     3241 cggcgcgggc ggtacggtga atgcgttgat cgccagctcg gcattccgca tcacgcatgg
     3301 cgagccgcgg cgcttcacca aggtggccga cagcggacgc acgctccacc ggttcttctg
     3361 cgccgattgc ggttcgccga tctacagtca gcgcgaagtc acgccggaaa tgatgaccct
     3421 gcgcgccggc acgctcgaca acgcgccgcc aatgaaggtc gtcgcgcata tctggaccgc
     3481 gagcgcgagg tcttgggcgc atatcgatcc ggcgacgcag cgctttcccg ggcagcccga
     3541 cgcgccggcc gtgaaaaagt aagaaggcgc ttttccgagc ttcgcgtagc atgcgggccg
     3601 tgaaagcgct ccctctcgtt ctcgcttttc ttgctgccgg ctgcaccagc gcgccgaacc
     3661 gcgcaccgga ggcgccgacg cgctcgatcc tcgcgagcgc ggaaatggcc gacgacgcgg
     3721 ccacgcatgc gctgaagatg gtcggccggc cctacaagta cggcggttct acgcccgccg
     3781 gcttcgactg cagcggactg attcggtaca gcttcggcag agcgggcctc tcgttgccgc
     3841 acggcactga caagcttcgc ggcgtttcga acgtcgtcaa gacttcggag ctgcggcgcg
     3901 gcgacctcct tttcttccac caggagggaa agcgctacgg gcacgccggc atctatctgg
     3961 ggaacgggcg cttcgtgcac gccccgtcgt cgggaaaatc agtgcgcacc gatgggcttg
     4021 acgcacccta ctggaaaagg cacctgagcg aggtgcggag actgtagatt tacggcatca
     4081 ggcagtatga gtatcaagct caaccacacc atcgttcatt cgcgcgatca gcgcgaggcg
     4141 gccgagttcc tcgcgagcct tttcggcttg ccggcgcccc agcccttcgg ccccttcctc
     4201 gatgtggacg tcgcgaacga ggtcacgctt gcattcctgg acgcggacgg catggaaatc
     4261 cagacgcagc actatgcctt cctggtaagc gatgacgagt tcgaccagat tttcgcgcgc
     4321 atccaggcgc gcggcctgaa gtactgggcc gatccgggac gcacggagga agggcgcatc
     4381 aaccatcact tcggcggccg cggcgtgtat ttccaggacc cgagcggcca cctgatggag
     4441 atcatcaccc gcccgtacgg cgcgttcccc aaggcgcgct agacttcgcc gtcccttcag
     4501 gggaggagta cccatgagga aactctatgc cgcgctgctc attctgcttt tcgctgttcc
     4561 ggtgcaagcc gtgaccttcg tctacgtctc caacgccgag gatggtgaca tcggccaata
     4621 catgatgcga ccggacggca cgctgcagcc ggtcacccgc tttaaggccg gccccaacgt
     4681 aatgccgatg agcgtcagtc cggacaagcg ttatctcgtc gccgccggcc gtgcgaagcc
     4741 gttcaccgct tacacgtacg cgatcgaccg cggcaccggc gcgctcaagc tcgtcggcac
     4801 ggggccgctc gcggaaagtt ttccgtacat cacgcacgac cgcaccgggc ggtggctgtt
     4861 gagcgcctcc tatggcggcc atctcgtcag cgtgaatccg ctgaagccgg acggcacggt
     4921 gggcgatcct caccaggtga taccgaccgc gcgcaacgcg cacgccatca tcagcgaccg
     4981 caccaaccgt cacgtgttcg tgccgcatct gggcacggat caggtgttcc agttcctttt
     5041 cgacgagaag agcgggcgcc tctcggcgag cacgccgccc gtcgtgcaga tgaaagccgg
     5101 catgggaccg cggcatctcg tgatgtcgcc ggataaccgc ttcgtgtatc tcctgaacga
     5161 gctcaccgcg acggtgacga cactcgcgaa cgaaaacggg ctcctcaggg aaatttccag
     5221 cgtgagcgcg ctgccgcccg ggagcacgct gcgccccggg atgccgcggc cgcagcccga
     5281 ccgcgacgtg tcgaacgacg tctgggcctc cgacatccat gtcgcgccga gcggacgctt
     5341 cgtctatgcg gcggaacgga cctcgagctc gatcgccgcg ttcagcgccg atccggccaa
     5401 cggcaagctc aactatctgg gcagtacgtc gaccgagaag cagccgcgcg gcttccgcat
     5461 cgacccgacc ggccgcttca tgatcgtttc cggcgagaag tccgacacga tctcgagcta
     5521 ccgcatcgag tcgagcggat tgctactgca ccttggcaaa tattcgacgg gaaaaggctc
     5581 gaactgggtc gagattgtca gctacgattg atccacagcc cgccaacagc ttatccacag
     5641 cttattgact attcgctact ctgcgcggcg cgcgatagcg ctgcacggtg cgcacttcct
     5701 cttcggtgat gatgtcgacg agccgcagaa agatctcgcg cggcagctcg cgccggctcg
     5761 gccagctctc gaagtcgctt tcgcttgcgc cgagatgcac tctaagcttt tcatttccgc
     5821 cgagaattgt cgctgcgcgc tggagtgcgc gccgatggat gtcttgcagc actgaacctc
     5881 ccttgttttc cccacgacag attgcgcttc acgtatagca atgtcaacga caacgatcag
     5941 gagatttgca tgtccgtagc gcgtgttgtt tttgtttctt tgctgagcgt gttcaccgcg
     6001 gccgcttgcg cgcagcaacc cgcgacgtcg acttacgagc cgaccgtcgg ccaggaagga
     6061 aaagatgtgg tgtgggtgcc gacgccgcag gcgctcgttg acaagatgct cgacatggcg
     6121 aaagtaacgg ctaacgattt cgtgatggac ctcggctccg gcgacggacg caccgtgatc
     6181 accgcggcaa aacgcggcgc gcgcgcggtc ggtgtggagt acaacccgca gatggtcgag
     6241 ctctccaagc gaaacgcgca gaaggaaggc gtgaccgatc gcgccgagtt catgaaagcc
     6301 gatctcttcg agaccgactt ttcgaaggcc accgtgatca cgatgttcct gctccctgac
     6361 atcaacctga agctgcggcc caagctcctg cagctgaagc ccggcacgcg ggtggtgtcg
     6421 aacagcttca ccatgggcga ctggcagccc gacgaaacgg cggagctcaa ttccgcgcag
     6481 ggctgcacca actcctggtg caccgcgctg ctctggatca ttccggcgcg cgtcgcgggc
     6541 acttacaaga ccgcgcaggg cgaagtcacg ttcaagcagc aataccagat gctgtccgga
     6601 acaatgcgca ccgcagagcg cacggtgccg atcgaaggca aggtgcgcgg cgaggacatc
     6661 acgttcaagg ccggcgggaa ggaataccgc ggccgactaa acggcaagca gctcgagctg
     6721 aagtcctagc gcgcgcgcgt catcagagct ctgcagacgg ggaccgcgat cgcgacgagt
     6781 gcgagcaccg tgagcgtcgc ggagatgggc cgcatgaaga ggatgaagat gcccgcgtcc
     6841 gagatgatga gcgcctggcg cagcgcgcgc tcggcgagcg ggccgagcac gagcgcgagc
     6901 acgagcgcgg ccggcgagta gccgagcttc ttcatgaagt agccgaccac gccgaacacg
     6961 agcatcatcc cgacgtccca gacgttgttc ttgagcgagt acgcgccggt cacgcagaac
     7021 accacgatga gcggcatcag gatcgaatag ggaatgcgca ccgctcgcac gaaaagcggg
     7081 atgaagagcg tcgacatgat cagaagcagc acgttggcga tgtactgcga ggcgatcagg
     7141 ccccagacga actgcggatt cttttcaaac agcatcggcc ccgggcgcag accccacatc
     7201 atgaggccgc cgagcatgat ggcggtggtg gccgaacccg gaatgccgag cgtcagcatg
     7261 gggaccatcg ccgccgtagc cgcggcgtta ttggcggact cgggcccggc gacgcccttg
     7321 atctcaccct tgccgaactt ctcgggctcg tccgagaccc gcttctcgac cgtgtaggcc
     7381 atgaaagcgg cgacggtcgg tcccgctccg ggcagcacgc cgaccagaaa tcccataagc
     7441 gtgccgcgcg cgatcgacca cttggttcgg acgaaatcct ggacgctggg gagtacgtcg
     7501 cgcaggcgca ggctcgtttt cacctgcgta gcgcccttct gctcggcgcc atagagcact
     7561 tcgcccaggc cgaagaggcc gatcgcgacc ggcaggaagt cgacgccgtc catcatttcc
     7621 gggatgccga aaccgaagcg cgcgtcgccc gagatgatgt cgaagcc
//
LOCUS       DASTPD010000158         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_288415, whole genome shotgun sequence.
ACCESSION   DASTPD010000158 DASTPD010000000
VERSION     DASTPD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288415"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VFR57_05480"
     CDS             complement(<1..1412)
                     /locus_tag="VFR57_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0258852.1"
                     /translation="MSEDTKTLDLFTPDGGEDEAPIEQHASQAYLGYAVSTVKARALP
                     EIADGLKPVQRRILYAMAGSTTFSKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSM
                     RYPLIDGQGNFGSRDGDAPAAYRYTEAKLSRFSELLLSEINEGTVDFARNYDGKFDEP
                     VLLPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAHVIKHARAKIEDVLDKLPG
                     PDFPGGGQVISPPEEIRQAYETGRGSLLLRCKWEKEALARGQWRIVITELPHGVSAKQ
                     VQQELDALADPKPGFGKKEVTQEQKRLKQFILDQVETVRDESDRKSKLRLVIEPRSSR
                     QDPEELMAALMVHTSLESRYAVNLTWLGLDGLPETKGIVEILREWGEWRVSTVRRRTE
                     FRLERVEERLEIVKGRLEAYAKIDDIINLIRVSEDQAEAKEKLRSKYKFTERQAQDIV
                     DLRLGQLTKLDGVKLNDEKKA"
     gene            complement(1423..1653)
                     /locus_tag="VFR57_05485"
     CDS             complement(1423..1653)
                     /locus_tag="VFR57_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258853.1"
                     /translation="MKRTSKNKHQPTWERAYKGHVLWLGRQKLGKVTLQGPGRYQWEG
                     GGRQGFAEELSKAKAAVELAAFTADKQLDLFK"
     gene            complement(1637..>2842)
                     /locus_tag="VFR57_05490"
     CDS             complement(1637..>2842)
                     /locus_tag="VFR57_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU0258854.1"
                     /translation="VEPGEGAAWAIGWVAEGDIFADSHVNLIPTRSGGTHEAGFRSGI
                     FEALAAFMDTRALAPKGVKVIADDLWQRACFTLSARIVRTQFHGQTKEKLTTRHAARL
                     LELCVRDAFELWLNEHPAEGTKIVELAIEQAMARLSKGKKVERKKSSGIATLPGKLVD
                     CASGDITRNELFLVEGDSAGGSAKEARDKETQALLPLRGKVLNTMSKDSRTVLANKEL
                     QAIATAIGVDPHSHEEGVDLSGVRYGKVIVMTDADVDGGHIQALLLTFFFMHAPKLVE
                     RGHLYVAMPPLYKVEVPSQGKGKPARRVYCLDDDELEETLAQLKKEKVKEGSWEISRF
                     KGLGEMSPEQLWETTMNPDTRRLVRMVLDQKEIKKVRETFEKLMDSGEAEGRRNWMRE
                     HWKTVEADV"
BASE COUNT          401 a          952 c          925 g          564 t
ORIGIN      
        1 gcctttttct cgtcgttcag cttcacgccg tcgagcttgg tgagctggcc gaggcgcagg
       61 tccacgatgt cctgcgcttg ccgctcggtg aacttgtact tcgaacgcag cttctccttc
      121 gcctcggcct ggtcttcgga gacgcggatc aggttgatga tgtcgtcgat cttcgcgtag
      181 gcctcgaggc ggcccttgac gatctccagg cgctcctcga cgcgctcgag gcggaactcg
      241 gtgcgccggc gcacggtgct gacgcgccac tcgccccact cgcgcaggat ctcgacgatg
      301 cccttcgtct ccggcaggcc gtcgaggccg agccacgtca ggttcaccgc atagcgcgac
      361 tcgaggctcg tgtggaccat gagcgcggcc atcagctcct cggggtcctg gcgcgaggag
      421 cgcggctcga tcacgaggcg cagcttcgac ttgcggtcgg actcgtcgcg caccgtctcg
      481 acctggtcga ggatgaactg cttcagccgc ttctgctcct gcgtgacctc cttcttgccg
      541 aagcccggct tcgggtcggc gagcgcgtcc agctcctgct gcacctgctt cgccgacacg
      601 ccgtgcggca gctcggtgat cacgatgcgc cactggccgc gcgcgagcgc ttccttctcc
      661 cacttgcagc gcaggaggag cgagccgcgc ccggtctcgt aggcctgcct gatctcttcg
      721 ggcggcgaga tcacctggcc gccgccgggg aagtccgggc cgggcagctt gtcgagcacg
      781 tcctcgatct tcgcgcgcgc atgcttgatg acgtgcgccg cggcggcggc cacttccttc
      841 aggttgtgcg acggaatgcg cgtggagaag ccgaccggga tgccgaagct gccgttcagc
      901 aacccgaacg gcaggcgcgc cggcagcaga acgggctcgt cgaatttgcc gtcgtaattc
      961 ctggcgaagt cgacggttcc ctcatttatt tccgacagaa gcaattcaga aaagcgggag
     1021 agcttcgcct cggtgtaccg gtacgccgcg ggcgcatcgc cgtcgcgcga gccgaaattg
     1081 ccctgcccgt cgatgagcgg atagcgcatc gagaacggct gcgcgaggtg cacgagggcc
     1141 tcgtacgtcg agctgtcgcc gtgcgggtga tacttgccga gcacctcgcc gacgtagcgc
     1201 gcgcacttgg agaacgtcgt cgacccggcc atggcataga ggattcgccg ctgcaccggc
     1261 ttcaggccgt cggcgatctc cggcagcgcg cgcgccttga cggtggagac cgcgtagccg
     1321 aggtacgcct gcgaggcgtg ctgctcgatc ggcgcctcgt cctcgccacc gtcgggcgtg
     1381 aagagatcga gggtcttggt gtcttcgctc atcgcgcttt tctcatttga agaggtccag
     1441 ttgtttgtcc gcggtgaacg ccgcgagttc tacggcggcc ttcgctttcg agagttcctc
     1501 cgcgaaaccc tggcgtccgc cgccttccca ctggtagcgg ccggggccct gcagtgtgac
     1561 ttttccgagc ttttgtcttc cgagccacag aacgtggccc ttatacgcgc gctcccaggt
     1621 tggctggtgt ttgttcttag acgtccgctt caacggtctt ccagtgctcg cgcatccagt
     1681 tgcgccggcc ctcggcctcg cccgagtcca tcagcttctc gaaggtctcg cgcaccttct
     1741 tgatttcttt ctggtcgaga accatgcgca cgaggcgccg cgtgtcgggg ttcatcgtcg
     1801 tttcccagag ctgctcgggg ctcatctcgc ccaggccctt gaagcgcgag atctcccacg
     1861 agccttcctt cactttctcc ttcttgagct gcgcgagcgt ctcctcgagc tcgtcgtcgt
     1921 cgaggcagta gacgcggcgc gctggcttgc ccttgccctg cgaaggaact tccaccttgt
     1981 agagtggcgg catggcgacg tagaggtggc cgcgctcgac gagcttcggg gcgtgcatga
     2041 agaagaaggt gaggagcagc gcctggatat ggccgccgtc gacgtccgcg tcggtcatga
     2101 cgatgacctt gccgtagcgc acgccgctca agtccacccc ttcttcgtgg gaatgagggt
     2161 caaccccaat ggccgtggcg atcgcctgca gctccttgtt ggcgagcacg gtgcggctgt
     2221 ccttggacat ggtgttgagc accttgccgc gcaagggcag aagcgcctgc gtctccttgt
     2281 cgcgcgcttc tttcgccgag ccgccggcgg agtcgccctc gaccaggaac agctcgttgc
     2341 gcgtaatgtc gcccgaagcg cagtcgacga gcttgccggg aagcgtggcg atgcccgagc
     2401 tcttcttgcg ctcgactttc ttccctttgg aaagccgcgc catcgcctgc tcgatggcga
     2461 gctcgacgat cttcgtgcct tcggcgggat gctcgttcag ccagagctcg aacgcgtcgc
     2521 gcacgcagag ctcgagcagc cgcgcggcgt ggcgcgtggt cagcttctcc ttggtctggc
     2581 cgtggaactg cgtgcgcacg atgcgcgccg agagcgtgaa gcaggcgcgc tgccagagat
     2641 cgtcggcgat caccttcacg cccttgggcg cgagcgcgcg cgtgtccatg aacgccgcga
     2701 gcgcctcgaa gatgcccgag cgaaagccgg cttcgtgcgt gccgcccgag cgcgtgggga
     2761 tgagattcac gtggctgtcc gcgaagatgt cgccttcggc gacccagccg atcgcccacg
     2821 ccgcgccctc gcccggctcg ac
//
LOCUS       DASTPD010000159         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_697692, whole genome shotgun sequence.
ACCESSION   DASTPD010000159 DASTPD010000000
VERSION     DASTPD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697692"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFR57_05495"
     CDS             <1..317
                     /locus_tag="VFR57_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU0258855.1"
                     /translation="LSAKRTAFFSVSLGAKERYATRFLQRARWKPDLVAVFPGALQYS
                     KYGPVKRLVVRAFAAVAGHDTDPSRDYEYTDWQAVERFSESFARLLSAKIDSHQGSGF
                     SV"
     gene            396..806
                     /locus_tag="VFR57_05500"
     CDS             396..806
                     /locus_tag="VFR57_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258856.1"
                     /translation="MNAFPRFLLAAAAIALGTAAIAAEPLKPTSLKGDAQRGAALHGG
                     CFSCHGTERYTAPVTNFFATLGDSVLRASGLSDLPPAEPKRFKGRAKSLDQLREAVWR
                     RNAYLNPPLAPQDVEDLVAYLNATYYKFPSANKP"
     gene            803..2065
                     /locus_tag="VFR57_05505"
     CDS             803..2065
                     /locus_tag="VFR57_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEU0258857.1"
                     /translation="MTTLRTKQISVVASSTLAFTVCFAVWMMFAVIGIPIKRELGLSE
                     TEFGLLAATPVLTGSLIRLPIGVWTDQFGGRIVFFVLMLVTVVPIWLIGEATQYWQFL
                     VLGLFVGLAGGSFSVGIAYCARWFEKQRQGFAMGIFGAGNSGAAVTKFVAPAIVVAFG
                     WAMVPKVYAAAMLVTALAFWMLSYSDPAHQQARKRSMREELAVLKDPNVWKLSQYYSI
                     VFGGYVGLSLWMTKYYVGEYGFELQSAALLAACFSLPGGVLRAIGGWMSDKWGAHPVT
                     WWVMLISFVCLFFLSYPQTDLVVRTTKGELSFHFGLNVYVFTTLMFVMGIAWAVGKAS
                     VFKYISDEYTHNIGVISGIVGLAGGLGGFLLPIMFGALLDVTGVHSTAFMLLWGITLV
                     SLVWMYWTEIVPLRRQQATALHAASQPA"
BASE COUNT          350 a          726 c          723 g          430 t
ORIGIN      
        1 acctgagcgc aaagagaacc gcattcttct cggtcagcct cggcgccaag gagcgttacg
       61 cgacccgctt cctgcagcgt gcgcgctgga agcccgatct ggtcgccgtg tttcccggtg
      121 cgctccagta cagcaagtat ggcccggtga aacggctggt cgtgcgcgcg ttcgcggccg
      181 tcgccggaca cgacacggac ccatcgcgcg actacgagta caccgactgg caggcggtag
      241 agcgcttcag cgaatctttc gcccgcctgc tttcggcaaa aattgattcg catcaaggca
      301 gcgggttttc cgtctgaagc ttgacgtagg tcaagcccgc accgcgcccc gatggtcgaa
      361 tgggttctcg tgatcaatcg atatcagggg acaccatgaa cgcgttcccg agattccttt
      421 tggccgcggc cgccatcgcg ctcggcacgg ccgcaattgc cgcagagccg ctgaagccca
      481 cgtctctcaa gggcgatgcg cagcgcggcg cggcgctgca cggcggatgc ttcagctgcc
      541 acggcaccga gcgctacacc gcgccggtga cgaacttctt cgcgaccttg ggcgactcgg
      601 tgctgcgagc gagcgggctg tcggacctgc cgccggcgga gccgaagcgc ttcaaggggc
      661 gcgcgaaatc gctcgatcag ctgcgcgagg cggtgtggcg gcgtaatgcg tatctgaacc
      721 cgccgctcgc gccacaggac gtcgaagacc tggtcgccta cctcaacgcc acctactaca
      781 agttcccaag cgccaataag ccatgacaac gctgcgcacc aagcagatct cggtcgtcgc
      841 ctcgagcacg ttggcgttca ccgtctgttt cgccgtctgg atgatgttcg ccgtcatcgg
      901 catcccgatc aagagggagc tcggcctgag cgagaccgag ttcggcttgc tcgccgcgac
      961 accggtgctc accggctccc tcatccgcct gccgatcggc gtgtggaccg accagttcgg
     1021 cggtcgcatc gtgttcttcg tactgatgct cgtcaccgtg gtgccgatct ggctcatcgg
     1081 cgaggcaacg cagtactggc agttcctggt gctcggcctc ttcgtcgggc tggccggcgg
     1141 ctcgttttcg gtcggcatcg cctactgcgc gcgctggttc gagaagcagc gtcagggctt
     1201 cgcgatgggc atcttcggcg ccggcaactc gggcgccgcg gtcaccaagt tcgtcgcccc
     1261 ggcgatcgtg gtggcgttcg gctgggcgat ggtgccgaag gtgtacgcgg cggcgatgct
     1321 cgtcaccgcg cttgctttct ggatgctcag ctatagcgat ccggcgcacc agcaggctag
     1381 gaagcggagc atgcgcgagg agctcgccgt gctcaaggac ccgaacgtct ggaagctgtc
     1441 gcagtactac tcgatcgtgt tcggcggcta tgtcgggctg tcgctttgga tgaccaagta
     1501 ctacgtcggc gagtacggct tcgagctcca atcggcggcg ctgctcgccg cgtgcttctc
     1561 gctgccgggc ggcgtgctgc gcgccatcgg cggctggatg tccgacaagt ggggcgcgca
     1621 cccggtgacg tggtgggtga tgctcatctc tttcgtgtgc ctgttcttcc tgtcgtaccc
     1681 gcagaccgac ctcgtcgtgc gcacgaccaa aggcgagctt tcgttccact tcgggctcaa
     1741 cgtctacgtc ttcacgacgc tcatgttcgt gatgggcatc gcctgggccg tcggcaaggc
     1801 ctcggtgttc aagtacatct cggatgagta cacgcacaac atcggcgtga tctcgggcat
     1861 cgtcgggctc gccggcggcc tcggcgggtt cctgctgccg atcatgttcg gcgcgctgct
     1921 cgacgtcacc ggggtgcact cgaccgcctt catgctgcta tggggcatca cgctggtctc
     1981 gctcgtctgg atgtactgga ccgagatcgt gccgctcaga cgacagcagg ccaccgcgct
     2041 gcacgcggcg tcgcaacccg cctaaggaga acaacgcatg ggtgccgcac tgccgggagt
     2101 gaagcttgag cccaagaaag ccagcggttc cgccgacatc gccgattggc ggccggagga
     2161 tccggctttc tgggaatcgg agggcaagcg gatcgcctac cggaaccttt ggatctcggt
     2221 tcccgccct
//
LOCUS       DASTPD010000160         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1514117, whole genome shotgun sequence.
ACCESSION   DASTPD010000160 DASTPD010000000
VERSION     DASTPD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514117"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..688
                     /locus_tag="VFR57_05510"
     CDS             2..688
                     /locus_tag="VFR57_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0258858.1"
                     /translation="MKTYRYYEFVMAAFVTILICSNLIGPAKIVQLDWLPFVFGAGVL
                     FFPISYIFGDILTEVYGYARARRVIWAGFGGLLFASVMASVVVALPPAPFWENQKAYE
                     IAFGTTWRIALASMFAYFCGEFANSFVLAKMKILTAGKWLWTRTIGSTIVGEAVDSSL
                     FYPLAFYNSGLIPNEKLPAIMLAQFGLKVAVEVVFTPITYKIVGALKRAEHEDYYDRG
                     TNFTPFSLKT"
     gene            685..1338
                     /locus_tag="VFR57_05515"
     CDS             685..1338
                     /locus_tag="VFR57_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258859.1"
                     /translation="MIPADLRLGELLARAGLLPRARLDAALERQAKHNGKLGALLVDL
                     GLIDEAELYAVLALQRSLHQAQAEDVILFLRARFGHILLGSAAITEHQLLRALLQQER
                     SGEPLGEILLRHGVISHAVQEAALGFQRTLASPLRERFRLGRMLLEAGIVDSTTLEAA
                     IRRQRGARVKLGDPLLEMGAITQDVLQAFLKRQRRLLAALSAGMAAAADAHGLPARS"
     gene            complement(1412..>3076)
                     /locus_tag="VFR57_05520"
     CDS             complement(1412..>3076)
                     /locus_tag="VFR57_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEU0258860.1"
                     /translation="YDQTCASEKRRRRKRNEYPDPAKRVVINEMVCEGCGDCSVKSNC
                     LSVEPLETEFGRKRTINQSSCNKDYSCLKGFCPSFVTVEGGRLRKGRSASRPVEEAFP
                     SLPEPAIPALKSSYGILVTGIGGTGVITIGQIVGVAAHLEGKGVSVLDMSGLAQKYGA
                     VMSHVQVAASPEHMHATRIPTGGADLVLGCDLVVAASTEAMEKMAPARTRALVNASVT
                     PTAEFVRNPNWQLPGTDLQKDVADSCKQAVFVPAAEVASGLMGDAIATNMFMLGYAYQ
                     KGWIPISAASLERAIELNGVAVDFNKKSFLWGRRAAVDLERVKRIAIPADVIPIEQHL
                     SRNLEELVERRVKFLAEYQDAAYAARYRALVEKTGKLEKDRVNSTKLTEAVARYYAKL
                     LAYKDEYEVARLHADAGFRAKIEGMFEGDYRVVYHLAPPLLARKDPTSGEPRKIRFGA
                     WMLPVFKALSSMRFLRGTALDPFGYTEERRTERALIREYEATVERLLARLTAQNHALA
                     VQIASIADEIRGFGHIKARSIDPARKKRDELLARFESGRGAERAAA"
BASE COUNT          481 a         1024 c          976 g          595 t
ORIGIN      
        1 catgaagacc taccgctact acgagttcgt gatggcggcg ttcgtcacga tcctcatctg
       61 ctcgaatctg atcgggccgg cgaagatcgt gcagctcgac tggctgccgt tcgtcttcgg
      121 cgccggagtg cttttcttcc cgatctctta catcttcggc gacatcctga ccgaggtgta
      181 cggctacgcg cgcgcgcggc gcgtgatctg ggcagggttc ggcgggctgc tcttcgcctc
      241 ggtgatggct tcggtggtgg tcgcgctccc cccggcgccc ttttgggaaa accagaaggc
      301 gtacgagatc gccttcggca ccacctggcg catcgcgctc gcctcgatgt tcgcctactt
      361 ctgcggcgag ttcgcgaact ccttcgtgct ggcgaagatg aagatcctga ccgccggcaa
      421 atggctctgg acgcgcacca tcggctcgac catcgtgggg gaggcggtcg actcctcgct
      481 cttctacccc ctcgccttct acaactccgg ccttatcccg aacgaaaagc tcccggcgat
      541 catgctggcg cagttcgggc tgaaggtcgc ggtcgaggta gtgttcactc ccattaccta
      601 caagatcgtg ggtgcgctaa agcgcgccga acacgaggac tactatgatc gcggcaccaa
      661 cttcactccc ttcagcctga agacatgatt cccgctgatc tccgccttgg cgagcttctt
      721 gcccgggccg gccttcttcc ccgggcgcgg ctcgacgccg ccctcgagcg ccaggcgaag
      781 cacaacggca agctcggcgc gctcctagtc gacctcgggc tgatcgacga ggccgagctc
      841 tatgcggtgc tcgctctgca gagaagcctg caccaggcgc aagccgaaga cgtcattctt
      901 ttcctgcgcg cgcgcttcgg ccacatcctg ctcggctccg ccgccatcac cgaacatcag
      961 cttcttcgtg cgctgctgca gcaggaacgc agcggcgagc cgctcggcga gatcctgctc
     1021 cgccatgggg tgatcagcca tgccgtccag gaggcagcgc ttggctttca gcgcactctc
     1081 gccagtccgc tgcgcgagcg cttccggctc ggccggatgc tgctcgaggc cggcatcgtc
     1141 gattcgacca cgctagaggc ggcgatccgc cgccagcgcg gcgcccgggt caagctcggc
     1201 gacccgctgc tcgagatggg cgccatcacc caggacgtgc tgcaggcgtt cctcaagcgc
     1261 cagcgccggc tcctcgccgc gctctctgcc ggcatggcag ccgccgccga cgcccacgga
     1321 ttgccagcgc ggagctgaaa atcggggtca gagcccgatt tccggtatct ccgctaccgc
     1381 caggagaaat cgggctctga ccccgatttt tttacgccgc ggcgcgctcg gcgccccgac
     1441 cggactcgaa gcgcgcaaga agctcgtcgc gcttcttgcg cgcggggtca atgctgcgcg
     1501 ccttgatatg gccgaagccg cggatctcgt cggcgatcga ggcaatctgc acggcgagcg
     1561 cgtggttctg cgccgtaagg cgggcgagga ggcgttcgac cgttgcctcg tattcgcgga
     1621 tcagcgcccg ctcggtgcgc cgctcttcgg tgtagccgaa cggatccaaa gccgttcccc
     1681 gcaagaacct cattgaagag agggctttga atactggaag catccacgcg ccgaagcgga
     1741 tcttgcgcgg ctcgccgctc gtcgggtcct tgcgcgcaag cagcggcgga gcgaggtgat
     1801 acacgacacg gtagtcgcct tcgaacatgc cttcgatctt cgcgcggaag ccggcgtcgg
     1861 cgtgcagccg cgcgacctcg tactcgtctt tgtaggcgag gagcttggcg tagtaccgcg
     1921 caacagcttc ggtgagcttg gtggaattga cgcgatcttt ttccagcttc cctgtttttt
     1981 ccacgagcgc tctataccgc gccgcgtagg cggcatcctg gtattcggcg aggaacttca
     2041 cgcgccgctc gacgagctcc tcgaggttgc gcgagaggtg ctgttcgatc gggatcacgt
     2101 cggccggaat cgcgatgcgt ttcactcgct cgagatccac cgctgcgcgc cggccccaga
     2161 ggaagctctt cttgttgaag tcgaccgcga cgccgttcag ctcgatcgcg cgctcgagcg
     2221 acgccgcgga gatcgggatc catcccttct ggtaagcgta gccgagcatg aacatgttgg
     2281 tggcgatcgc atcgcccatg agtccgctgg cgacttccgc cgccgggaca aataccgctt
     2341 gcttgcagga atcggcgaca tctttctgca ggtcggtgcc gggcaattgc cagttcgggt
     2401 tgcgcacgaa ttccgcggtc ggcgttaccg aagcgttgac gagcgcgcgg gtgcgcgccg
     2461 gcgccatctt ttccatcgcc tcggtgctcg cggcgacgac caggtcgcag ccgaggacga
     2521 gatccgcgcc gccggtgggg atgcgggtcg cgtgcatgtg ctcggggctg gccgcgactt
     2581 gcacgtgcga catgaccgcg ccgtacttct gcgcgagtcc ggacatgtcg agcacgctca
     2641 cgcccttgcc ctcgaggtgc gccgccacac cgacgatctg gccgatggtg atgacgccgg
     2701 ttccgccgat gccggtcacc aggattccat aggaagattt cagggccgga attgccggct
     2761 cgggcagcga agggaaggcc tcttccacgg gcctggaagc cgatcttcct ttacgtagac
     2821 gaccgccctc gacggtgacg aagctcgggc agaagccctt caggcaggaa tagtccttgt
     2881 tgcacgagga ctggttgatg gtgcgcttgc gcccgaattc cgtttcaagc ggctcgaccg
     2941 agaggcagtt cgacttcacg ctgcagtccc cgcagccctc gcacaccatc tcgttgatca
     3001 cgacgcgctt cgccggatcg gggtattcgt tcctcttgcg gcggcggcgc ttctcggagg
     3061 cgcaggtctg gtcgta
//
LOCUS       DASTPD010000161         9337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_901737, whole genome shotgun sequence.
ACCESSION   DASTPD010000161 DASTPD010000000
VERSION     DASTPD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901737"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFR57_05525"
     CDS             <1..215
                     /locus_tag="VFR57_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0258861.1"
                     /translation="TGSTGAVGQSLDVLHALGLALAAGPQRWTAMEGGWPIMAGGECI
                     GAVGVAGGNWEFDEKAAREAAESISK"
     gene            212..1198
                     /locus_tag="VFR57_05530"
     CDS             212..1198
                     /locus_tag="VFR57_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0258862.1"
                     /translation="MNVACLGMGWWSDVLADAIKRSGKLHIVSCFSRSQEKREAFAKK
                     YGCRAARSYDEILEDRSIEAIINTTPNSVHRETTVAAARAGKHTFLDKPIANTIEDAR
                     VITEACRRAKVVLALGYQRRRESHFRWVKEKIAAGTFGRLVNAEANISRDRLGKIDLS
                     SWRYTAEGMPGGVMLQIGIHYTDVLEYLIGPIKAVSGRFVRLVLPGDNPDVASLVLEH
                     ENGALSTLNASYASASEYYLMNIYGKEASAYYDLHHGLRFLKRGSTTSEPVPVRKNDP
                     IVEELEEFAAAVRGEAEPEMDGARGITSLAVLLAGIESAKEGRRVEVREVLG"
     gene            1288..2139
                     /locus_tag="VFR57_05535"
     CDS             1288..2139
                     /locus_tag="VFR57_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258863.1"
                     /translation="MLFPAGSSADVTARMLADGMSRQLGQRVLVVNRPGAGGAIGYKH
                     VAAQKPDGYSLVWNSNSISTTFHSGQLNFDYKAFDAVARVLSESVVLAVRNDAPWQTL
                     NELIQEAKAKPKAVSVGHSGIGSHTHISLAALVNAAGIEVNEVPFAAAQVVPSLVGGH
                     VNAVVQFPGALAAPLKQGQVRLLATLTQSRDPAWPEVPTARELGVDAVLDAWRGIAVP
                     RGTPRPVVSLLESAIRMTATSPEFVAQSEKLGVRPAFLPAEEFSALLAKEDAELSRLM
                     QRLGLKK"
     gene            complement(2069..3991)
                     /locus_tag="VFR57_05540"
     CDS             complement(2069..3991)
                     /locus_tag="VFR57_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0258864.1"
                     /translation="MRALKWIGGAVLVLFVAIALFFTFGLNALRGPITSAVTKATGRE
                     LVIAGELKPVWSWLHPRIRAERVTFANAAWGKADYLLDADAIEASITLLPLLAGRVVL
                     PEVHLERAKLALEQDAEGRKNWILKEEPEAEKEESRFFIKLLTLDEALLRWEDAARKH
                     SLAVDLSTDETGVQFAAVGSYGGKPLKASGHAGHVLSIRDQKTPFPLKAEASIGGTSA
                     RLEGTLTGIVGFKGIDLRFERLAGKSMDELYDIIGLAFPATSPYAVSGRLYRTDGVWL
                     FEDFTGKVGESDLAGWIRVQTGAGKRPFMQGDVTAKVLNFADLGALVGTNRPRKKGVL
                     PDMPFSPERWESVDADVKIDAGTIKRPEQLPIERFATRLLLRDRVLTLDPLSFGIAGG
                     RLAGTVRLDGNQEPMRGEVKMRVQNLQLAKMFPRIEQTKSSVGDLDGLIELSGSGDSV
                     GKLLASANGKIGVYIDQGRISQLLMELVALDLWDVARIKLRGDKDIDIRCAIADFGVK
                     DGVMQANALIFDTSVVNVGGSGSINLKDEQMDLTLKPQPKDGSVASLRTPLYIRGTFG
                     EPDIGPNVGRIAARGAGVLAMGILNPLLAILPLLEEGKGKDSNCAGLIKEAATSSGRA
                     SASGGTTPRPPSPAKH"
     gene            complement(4113..4577)
                     /locus_tag="VFR57_05545"
     CDS             complement(4113..4577)
                     /locus_tag="VFR57_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU0258865.1"
                     /translation="MKVTLLVGTMTGTAQLCAQEMELALDDGETEVETVMMDNLDASV
                     FSRPSVFLICTSTYGQGDVPDNARALYEDLKQARPDLSHVRYGVFGLGDRTYAETFNF
                     GGKRFDDILTELGARRVGERHMHDASSGILPEETALEWCQQWLTKVKEAQTA"
     gene            4628..5362
                     /locus_tag="VFR57_05550"
     CDS             4628..5362
                     /locus_tag="VFR57_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0258866.1"
                     /translation="MTETAYLSLLGDRVRAWRNEQGVTRKALSAASGVSERYLAQLEA
                     GEGNISVLLLRKVARAMCVPVESLVREGERQHKPIALLGLRGAGKSTLGEKLAQALDV
                     PFVELDREVEKEAGAELGEVFAMYGQEGFRRFERRALERVLGQHDRAVIATGGSLVTD
                     GGTYKLLRERSLCVWLKASPEEHMARVVAQGDMRPFKGSSAALDEIRQLLADRDRLYG
                     RADLVLDTSGKTVRSSLSQLISLVKT"
     gene            5359..6927
                     /gene="boxC"
                     /locus_tag="VFR57_05555"
     CDS             5359..6927
                     /gene="boxC"
                     /locus_tag="VFR57_05555"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEU0258867.1"
                     /translation="MISFETQPDRYHHWKLGFDGPVATLTLDVAEDKPLAPGYKLKLN
                     SYDLGVDIELADALNRIRFEHPEVKSVVITSGKSRMFCSGANIYMLGHSSHAWKVNFC
                     KFTNETRNGMEDASAQSGLKFLAALNGTTAGGGYELALACDEIAMIDDRSSTVSLPEV
                     PLLGVLPGTGGLTRLVDKRKVRRDLADVFCTTAEGVRADRAKQWRLIDHMEKPQKFAE
                     MVKARALELAKQSDRPGGEGVALTPLGANRLIDVTIDSQTRTATLSVKGPEAVELERS
                     TEWYPLRLARELDRAILDLRHNHLDIGLWVLKTQGNLKSILELDALLQQQEGDWFVRE
                     TIGYLRRTLSRLDVSSRSIFAMIEKGSCFAGTLMELALAADRSYMLDIEDGPSIALSA
                     MNFGAYPMANGRSRLETHFCGEMPSLEKGKPFSTDQARKLGLVTSTPDDIDWDDEVRI
                     AIEERASLSPDALTGMEASLRFAGPETMLTRVFGRLTAWQNWIFNRPNAVGEQGALKV
                     YGTGAKAKFNWERV"
     gene            6927..8339
                     /gene="boxB"
                     /locus_tag="VFR57_05560"
     CDS             6927..8339
                     /gene="boxB"
                     /locus_tag="VFR57_05560"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEU0258868.1"
                     /translation="MSSINYSEKIPNNVNLVNDRTLQRALEHWQPHFLDWWKNMGPSD
                     FQAADVYLRTAISVDAQGWAHYGTVKMPDYRWGIFLANPVAERKIGFGDQMGQPAWQQ
                     VPGEYRSTLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHAYFGRDGREEAEELLARHSGDADKPRILTTFNEPISDWLSFYCFTYFTDRDGKY
                     QLKSLAESAFDPLSRTCRFMLTEEAHHMFVGETGIGRVVKRTLEVMKELGTDDPDKVR
                     AAGAVDLPTMQKYLNFWCSSSLDLFGSEISSNSAANFANGLKGRPDESQYEDHVLREA
                     TMTLPTPEGSQTVPMLNALNEVMRESYLKDCEIGLKRWNRQIERAGFPFRLTLPSTRF
                     RRSIGAWAGQPVDPQGNMISKESHEKSLANWIPSESDKAFVHSLMQKVAAPGKMAAWI
                     APPERGINNLPVDYEYVKLH"
     gene            8353..8736
                     /locus_tag="VFR57_05565"
     CDS             8353..8736
                     /locus_tag="VFR57_05565"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEU0258869.1"
                     /translation="MLEAERAGAKALVVYMDDFPRNSEAWKILRQIQADEAHNCALIG
                     KLLEKSGTPYSHATGKFYDKAVAVKGRRERIEFLVRGLKWAVKKFEQALPALDPEARD
                     VFTKMRDSHLRTIAACERLAQALPA"
     gene            complement(8718..>9337)
                     /locus_tag="VFR57_05570"
     CDS             complement(8718..>9337)
                     /locus_tag="VFR57_05570"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU0258870.1"
                     /translation="LGVELLPLIAGLGIAGAGIALAMQGVLGNAVAGLTIIFTHPFRV
                     GDYISIVKEEGEVLEIKLFNTTLGHPDLSRVVIPNRKIVGEILHNYGRIRQLSIEVGV
                     AYDTDVAVGLKAAEDALRANPRVLKDPAPLVRVAKFADSKVVILVAPWVSVPDYGPAG
                     AELYPAILEEFRRRGIVIPFPQRDIRVVSSTAYVGKKEGTYAGSA"
BASE COUNT         1712 a         3065 c         2994 g         1566 t
ORIGIN      
        1 acaccggctc caccggcgcg gtcggccagt cgctcgacgt gctgcacgcc ctgggccttg
       61 cgctcgccgc aggcccgcag cgttggaccg cgatggaagg tggctggccc atcatggcgg
      121 gcggcgaatg catcggcgcc gtgggcgtcg cgggcggcaa ctgggagttc gacgaaaaag
      181 ccgcgcggga ggccgcggag tccatcagca aatgaacgtc gcgtgcctcg gcatgggctg
      241 gtggtcggac gtgctcgccg atgcgatcaa acgctcgggg aagctgcata tcgtgtcctg
      301 tttctcccga tcgcaagaaa agcgagaagc gtttgcgaag aagtatggct gccgggcggc
      361 gcgatcctac gacgagattc tcgaggacag gagcatcgag gccatcatca acaccacgcc
      421 gaactcggtg catcgcgaga ccaccgtcgc ggccgccaga gccggcaagc acaccttcct
      481 cgacaagccg atcgccaaca ccatcgaaga tgcgcgcgtc atcacggagg cgtgccgcag
      541 ggccaaggtg gttctcgcgc tgggatacca gcggcggcgg gagtcacatt ttcgctgggt
      601 gaaggagaaa attgcggctg gcactttcgg caggctcgtc aacgcagaag ccaacatcag
      661 ccgcgaccgg ctgggcaaga ttgacctcag ctcgtggcgc tacaccgccg agggcatgcc
      721 gggcggcgtg atgctgcaga tcgggatcca ctacaccgat gttctggagt acctgatcgg
      781 gccgatcaag gcggtgagcg ggcgtttcgt gcggctcgtg cttcctggcg acaacccgga
      841 tgtcgcgagc ctcgtgctcg agcatgaaaa cggcgcgctt tccacgctca acgcgagcta
      901 cgcctcggcg tccgagtatt acctgatgaa tatctacggc aaggaagcga gcgcctatta
      961 cgatctgcac catgggctgc ggttcttgaa gcgcggcagt acgacctcgg agccggtgcc
     1021 cgtcaggaaa aacgacccga tcgtcgagga gctcgaggag ttcgctgccg cagtgcgcgg
     1081 cgaagccgag ccggaaatgg atggcgcgcg cggcatcacg tcgctggcag tgctgctcgc
     1141 aggcatcgag tcggcaaaag aaggcaggcg cgtcgaggtg agggaggtat tgggatgaaa
     1201 aatttattgt tcttgtgcgg gtggatcgct ttttcggctt ttgctcagga acccccgttt
     1261 ccccagcgtg gaccgctcga gatcacggtg cttttccccg ccggctcttc cgccgacgtg
     1321 acagcgcgca tgctggccga cgggatgtcg cgccaactcg ggcagcgcgt gctggtggta
     1381 aaccgcccgg gcgcaggtgg tgcgatcggc tacaagcacg tcgcggccca gaagcccgac
     1441 ggctactcgc tggtatggaa ctcgaattcg atctcgacca ccttccattc cgggcagctc
     1501 aatttcgact acaaggcgtt cgacgccgtg gcgcgcgtgc tgtcggagtc ggtggtgctc
     1561 gcggtcagga acgatgcgcc gtggcagacg ctgaatgagc taatccagga agccaaggca
     1621 aaaccgaagg cagtttcggt cggacactcg ggcatcggca gccataccca catctcgctc
     1681 gcagcgctcg tcaacgccgc cgggatcgaa gtcaacgaag tgcccttcgc cgccgcgcag
     1741 gtggttccga gcctggtcgg cggccatgtg aacgcggtgg tgcagttccc gggcgcgctc
     1801 gccgcgcctc tcaagcaggg tcaggtccgc ctgctcgcaa cgctgacgca gagccgcgac
     1861 cccgcgtggc ccgaggtgcc gacggcgcgc gagctcggcg tggacgccgt gctcgacgct
     1921 tggcgcggca tcgcggtgcc gcgcggcacg ccgcgcccgg tagtgagcct gctcgaaagc
     1981 gcgatcagga tgaccgcaac ctcgcccgag ttcgtcgcgc agagcgagaa gctcggcgtg
     2041 cggccggcgt tcctgccggc cgaggaattc agtgctttgc tggcgaagga ggacgcggag
     2101 ttgtcccgcc tgatgcagag gctcggcctg aagaagtagc ggcctccttg atcagccccg
     2161 cgcaattgga gtccttgccc ttgccttcct cgagcaaagg cagaatcgcg agcagcggat
     2221 tcaggattcc catggccagc acgcccgcgc cccgcgcggc gatccggccc acattggggc
     2281 cgatgtcggg ctcgccgaaa gtaccgcgaa tgtaaagagg cgtgcgtagg gacgcgacgc
     2341 tgccgtcctt cggctgcggc ttgagcgtga ggtccatctg ttcgtctttc aggttaatgc
     2401 tgccgctgcc gcccacgttg accaccgagg tgtcgaagat gagcgcgttc gcctgcatca
     2461 cgccatcttt cacgccgaag tcggcgatcg cgcagcggat gtcgatgtcc ttgtcgccgc
     2521 gcagcttgat gcgcgccacg tcccacagat cgagggcgac gagctccatc agcaactggc
     2581 tgatccggcc ctggtcgatg tagacgccga tcttgccgtt cgccgacgcg aggagcttgc
     2641 cgaccgagtc gccgcttccc gagagctcga tcagcccgtc caggtcgcca accgacgatt
     2701 tcgtctgctc gatcctgggg aacatcttcg cgagctgcag gttctgcacc cgcatcttca
     2761 cctcgccgcg catcggctcc tggttgccgt cgaggcgcac cgtgccagcg agcctgccgc
     2821 ccgcgatgcc gaagctgagc ggatcgagcg tgagcacgcg gtcacgcagc aagaggcgcg
     2881 tggcgaaccg ttcgatcggc agctgctccg ggcgcttgat cgtacccgcg tcgatcttga
     2941 cgtccgcgtc cacgctctcc cagcgttccg gggaaaaagg catgtcgggg aggactccct
     3001 ttttgcgcgg ccggttggtg ccgaccagcg caccgagatc ggcgaagttg agaaccttgg
     3061 ctgtgacatc gccctgcatg aagggacgct tgcccgcgcc ggtctgcact cggatccagc
     3121 ccgccaggtc gctttcgccg accttgccgg tgaagtcctc gaacagccat acgccgtcgg
     3181 tgcggtaaag acgcccagac accgcgtagg ggctggtcgc cgggaacgcg agcccgatga
     3241 tgtcgtagag ctcgtccatc gacttcccgg cgaggcgctc gaagcgcagg tcaattccct
     3301 tgaacccgac aatcccggta agcgtccctt ccaggcgggc cgacgttccc ccaatgctcg
     3361 cctccgcttt cagcggaaag ggcgtcttct ggtcgcggat cgagagcacg tgccctgcgt
     3421 gcccgctcgc cttgagcggc ttgccaccgt acgagcccac cgccgcgaac tgcacgcccg
     3481 tctcgtcggt tgacagatcg accgcgaggc tgtgcttgcg ggcggcgtct tcccagcgca
     3541 gcagcgcttc gtcgagcgtg agcagcttta tgaaaaagcg cgattcctct ttctcggctt
     3601 cgggctcctc cttcagaatc cagttcttgc gtccctcggc atcctgctcg agcgccagct
     3661 tggcgcgttc gaggtgcacc tccggcagca caacgcgccc ggcgaggagc ggcagcaggg
     3721 taatgctcgc ttctatggcg tcggcgtcga ggagatagtc cgcctttccc caggccgcgt
     3781 tggcgaaagt gacgcgctcc gcccggatgc gcggatgcag ccagctccac acgggcttaa
     3841 gctcgcctgc gatcacaagc tctcgcccgg tcgccttcgt caccgcgctc gtgatcggac
     3901 cgcgcagcgc gttcaggccg aatgtaaaaa agagcgcgat tgcaacgaag aggacgagaa
     3961 cagcgccgcc gatccacttc agagcacgca cgacgagtaa acagcaaagc gcgtgccgga
     4021 agcaaaatcg gggtctgagc ccggtttgcc gtagcgagag cggaactacc ggaaatcggg
     4081 ctctgacccc gattttcttg tcgggagttt gcttacgccg tctgcgcttc cttcactttc
     4141 gtaagccatt gctggcacca ttccagcgcc gtctcctcgg gcaggatgcc gctcgaggcg
     4201 tcgtgcatgt ggcgctcgcc gacgcgccgg gcgccgagct cagtgaggat gtcgtcgaag
     4261 cgcttgccgc cgaagttgaa ggtttcggcg taggtgcggt cgccgagccc gaacacgccg
     4321 tagcgcacgt gcgacaggtc ggggcgcgcc tgcttgaggt cctcgtacag cgcgcgcgcg
     4381 ttgtcgggca cgtcgccctg tccataagtc gaagtgcaga tgaggaagac cgaaggccgg
     4441 gaaaagaccg acgcgtccag attgtccatc atcaccgtct cgacctcggt ctccccgtcg
     4501 tcgagcgcga gctccatttc ctgcgcgcaa agctgcgccg tgccggtcat cgtgccgacc
     4561 aggagggtga ctttcattga cagacaatat agtgcaccaa tagaatgcag gtcaagcatt
     4621 atcctgcatg actgaaacgg cctacctctc cctcctcggc gaccgcgtgc gcgcctggcg
     4681 caacgaacag ggcgtgacgc gcaaggcgct gtccgccgcg agcggggttt ccgaacgcta
     4741 cctcgcccag ctcgaggccg gcgaggggaa catttcggtg cttctgctgc gcaaggtggc
     4801 gcgcgcgatg tgcgtgccgg tcgaaagcct ggtacgcgaa ggcgagcggc agcacaagcc
     4861 catcgccctc ctcgggctgc gcggcgccgg gaaatcgacg ctcggagaga agctggccca
     4921 ggcgctcgac gtcccgttcg tcgagctcga ccgggaggtc gagaaggaag ccggcgccga
     4981 gctcggcgag gtgttcgcca tgtacggcca ggaaggcttt cgccgcttcg agcgccgcgc
     5041 gctcgagcgc gtgctggggc agcacgaccg cgccgtcatc gccaccggtg gcagcctcgt
     5101 caccgacggc ggcacttaca agctgctgcg cgagcgctcc ctgtgcgtct ggctgaaagc
     5161 atcgccggag gagcacatgg cgcgcgtcgt cgcgcagggc gacatgcgtc cgttcaaggg
     5221 aagttcggct gcgctcgacg agattcgcca gctcctcgcc gaccgcgacc ggctctacgg
     5281 ccgcgcagac ctcgtgctcg acacctccgg taaaacggta cgctcgagcc tttcgcagct
     5341 gatctcactc gtgaagacat gatctcattc gaaacgcaac ccgaccgcta ccaccactgg
     5401 aaactcggat tcgacggccc ggtggccacc ctcacgctcg acgtggccga ggacaagccg
     5461 cttgctcctg gctacaagct caagctcaac agctacgacc tcggcgtcga catcgagctc
     5521 gccgacgcgc tcaaccgcat ccgcttcgag catcccgagg tgaagagcgt cgtgatcacc
     5581 agcggcaaga gccgcatgtt ctgctcgggc gcgaacatct acatgctcgg ccactcgagc
     5641 cacgcatgga aggtgaactt ctgcaagttc accaacgaga cgcgcaacgg catggaggac
     5701 gcctcggccc agtccggact caagttcctc gccgcgctga acggcaccac cgccggcggc
     5761 ggctacgagc tcgccctcgc ctgcgacgag atcgcgatga tcgacgaccg ctcctccacc
     5821 gtctcgctgc ccgaagtgcc gttgctcggg gtattgcccg gcaccggcgg cctgacgcgc
     5881 ctcgtggaca agaggaaagt gcgccgcgac cttgccgacg tcttctgcac caccgcggag
     5941 ggcgtgcgcg ccgaccgcgc caagcagtgg cggctcatcg accacatgga gaagccgcaa
     6001 aaatttgccg agatggtcaa ggcgcgcgcg ctcgagctcg ccaagcagtc cgatcgaccc
     6061 ggtggcgagg gcgtggcgct caccccgctg ggcgccaatc gcctgatcga tgtgactatc
     6121 gattcgcaaa cccgcacggc cacgctcagc gtgaaaggcc cagaggcggt ggagctcgag
     6181 agaagcaccg agtggtatcc gctgcgcctt gcgcgcgagc tggaccgcgc aatcctcgac
     6241 ctgcgccaca accacctcga catcggcctc tgggttttga aaacccaggg caatctgaag
     6301 agcattctcg agcttgatgc gctccttcaa caacaagaag gcgactggtt cgtgcgcgag
     6361 actatcggct atctccgccg cacgctctcg cgcctcgacg tctcctcgcg ctcgatcttc
     6421 gccatgattg agaagggctc gtgcttcgcc ggcacgctca tggagctcgc gctcgcggca
     6481 gaccgtagct acatgcttga tatagaggac ggtccgagca tcgcactctc cgcgatgaac
     6541 ttcggcgcat atccgatggc gaacggccgt tcgcggctcg agacgcactt ctgcggcgag
     6601 atgccttcgt tggagaaagg aaaaccgttc tcgaccgacc aggcaaggaa gctgggattg
     6661 gtcacctcca cacccgacga tatcgactgg gacgacgagg tgcgcatcgc tatcgaagag
     6721 cgcgcgagcc tctcgcccga cgcgctcacc ggcatggagg cgagcctgcg gtttgccgga
     6781 ccggagacga tgctcacgcg cgtcttcggc cgccttacgg cgtggcagaa ctggatcttc
     6841 aaccggccga acgccgtcgg cgaacaaggc gcgctcaagg tctacgggac cggcgcaaaa
     6901 gcgaaattca actgggaaag agtctaatga gcagcatcaa ctattcagaa aagattccga
     6961 acaacgtcaa cctggtgaac gaccgcacgc tgcagcgcgc gctcgagcac tggcagcccc
     7021 acttcctcga ttggtggaag aacatggggc cgagcgactt ccaggcggcc gacgtgtatc
     7081 tgcgcaccgc gatctcggtc gacgcgcagg gctgggcgca ctacggcacg gtcaagatgc
     7141 ccgattaccg ctggggcatc ttcctcgcca acccggtcgc cgaacgcaag atcggcttcg
     7201 gcgaccagat ggggcagccg gcatggcagc aggtacccgg cgaataccgc tcgacgctgc
     7261 gccggctcat cgttacgcaa ggggacaccg agcccgcctc ggtcgagcag cagcgcctgc
     7321 tcgggcacac ctgcccttcc ctctacgact tgcgtaacct ctttcaggtc aacgtcgagg
     7381 aaggccgcca cctttgggcg atggtgtacc tcctgcacgc ctactttggc cgcgacggcc
     7441 gcgaggaagc cgaggagctg ctcgcgcggc actcgggcga tgcggacaag ccgaggatcc
     7501 tcaccacgtt caacgagccg atctcggact ggctgtcgtt ctactgcttt acctacttca
     7561 ccgaccggga cggcaagtac cagctgaaga gcctcgccga gagcgcgttc gatcccttgt
     7621 cacgcacgtg ccggttcatg ctcaccgagg aggcgcacca catgtttgtc ggcgagaccg
     7681 gcatcggccg cgtggtgaag cgtacgctcg aagtaatgaa ggagctcggc accgacgacc
     7741 cggacaaggt gcgcgccgcc ggcgcggtcg atctgccaac gatgcagaaa tacctcaact
     7801 tctggtgctc gagctcgctc gacctgttcg gctcggagat ctcttccaac tccgcggcca
     7861 atttcgccaa cgggctcaag gggcgcccgg acgaatcgca gtacgaggac catgtccttc
     7921 gtgaagcgac catgacgctc ccgactccag aaggcagtca gacggtgccg atgctgaacg
     7981 cgctgaacga ggtcatgcgc gagtcttacc tcaaggattg cgagatcggg ctcaagcgct
     8041 ggaaccggca gatcgagcgc gccggcttcc cattccggct caccctgccc tccacgcgct
     8101 tccgcagatc gatcggcgct tgggccgggc agccggtgga tccgcaaggc aacatgattt
     8161 caaaggaaag ccatgaaaaa agcctggcaa actggattcc gtccgagtcc gacaaggcgt
     8221 tcgtgcactc gctgatgcag aaagtcgccg cccccggcaa gatggcggct tggatcgcgc
     8281 cgccggagcg cggcatcaac aacctgccgg tggactacga gtacgtcaaa ctccactaaa
     8341 tttttcaacg agatgctcga ggccgagcgc gcgggcgcga aggcgctcgt cgtctacatg
     8401 gacgactttc cgcgcaattc cgaagcgtgg aagatcctgc gccagatcca ggccgacgag
     8461 gcgcacaact gcgcgctcat cggcaagctg ctcgagaagt ccggcacgcc ttacagccat
     8521 gccaccggca agttctacga caaggcggtg gcagtgaagg ggcggcgcga gcgcatcgaa
     8581 tttctcgtgc ggggattgaa gtgggccgtg aagaaattcg agcaagcgct gccagcgctc
     8641 gaccccgagg cgcgcgacgt gttcactaag atgcgcgact cgcacctgcg cacgatcgcc
     8701 gcctgcgagc ggttggctca ggcgctgccg gcgtaggtac cctccttctt tccgacatat
     8761 gcggtgctcg aaacgacgcg gatgtcgcgc tgcgggaacg ggatgacgat gccgcgccgc
     8821 cggaactcct cgaggatcgc cggatacagc tcggcgccgg cggggccgta gtcgggcacg
     8881 ctcacccagg gcgccaccag aatgaccacc ttcgagtccg cgaacttggc gacccggacg
     8941 aggggagccg ggtccttgag cacgcgcgga ttggcgcgca gcgcgtcctc cgccgccttc
     9001 agcccgaccg cgacgtcggt gtcgtacgcc accccgacct cgatcgacag ctggcggatc
     9061 ctgccgtagt tatgcaggat ctcgcccacg atcttgcggt tcggaatcac cacgcgcgac
     9121 aggtccgggt gtccgagcgt ggtattgaag agcttgatct cgagcacctc gccctcctcc
     9181 ttgacgatcg agatgtagtc gcctacgcgg aatgggtgcg taaagatgat cgtcagcccg
     9241 gcaactgcgt tgccgaggac gccctgcatg gcgagcgcga tcccggcccc ggcgatgccg
     9301 agtcccgcga tcagcggcag cagctcgacg ccgaggt
//
LOCUS       DASTPD010000162         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1310144, whole genome shotgun sequence.
ACCESSION   DASTPD010000162 DASTPD010000000
VERSION     DASTPD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310144"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFR57_05575"
     CDS             <1..257
                     /locus_tag="VFR57_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU0258871.1"
                     /translation="PGAGHYGPMSIHYHYFTLEQRDALEAQIRARTSSDPALRLALER
                     LHQPDYGVCIECGKDIAFVRLQADPDAVHCADCARLPVKR"
     gene            complement(247..1410)
                     /gene="prpC"
                     /locus_tag="VFR57_05580"
     CDS             complement(247..1410)
                     /gene="prpC"
                     /locus_tag="VFR57_05580"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEU0258872.1"
                     /translation="MAGLTEAVPKPKKSVALSGVAAGNTVLCSVGRTGNDLHYRGYDI
                     LDFADRAEFEEIAYLLVHGDLPTKDELGEYKKKLCRLRRLPEMLKAALECVPETAHPM
                     DVLRTGVSVLGTLEPEKDDHGQAGAQHIADRLMASLGSMLLYWYHFHAEGRRVDVETD
                     DDSIGGHFLHLLHGKPPSPAHVRAMHASLILYAEHEFNASTFACRVIAGTGSDMYSAI
                     GAGIGALKGPKHGGANEAAFAVLSRYKDPDEAERDIVRRLQAKEVVIGFGHPVYTISD
                     PRNEVIKGVARRLAKDSATQLLFAIAERLEKVMWREKKMFPNLDWYSAVSYHAMGVPT
                     DMFTPLFVISRTSGWAAHVIEQRADGKIIRPSANYIGPENRKWTPIEGRAASA"
     gene            complement(1398..2192)
                     /locus_tag="VFR57_05585"
     CDS             complement(1398..2192)
                     /locus_tag="VFR57_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258873.1"
                     /translation="MLRVNNIEVIYDHVILVLKGVSLAVREGGIVALLGANGAGKTTC
                     LKAVSNLLGAERGEVTKGSIEFQGERVERLNPSELVRRGVIQVMEGRHCFGHLTVDEN
                     LLTGAHTRHSNRFEIKRDLDRVYSYFPRLKDRRSSLAGYISGGEQQMTAVGRALMARP
                     KMILLDEPSMGLAPQIVQEIFEIVQNLNRREKVSFLLAEQNAVMALKFAEFGYILESG
                     RVVIEGTSAELAENEDVKEFYLGISSGKQKSFRDVKHYRRRKRWLA"
     gene            complement(2195..>2807)
                     /locus_tag="VFR57_05590"
     CDS             complement(2195..>2807)
                     /locus_tag="VFR57_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0258874.1"
                     /translation="VMNPTALKAAQKVGYKTERIVGVWWSGAEEDVLPAGDAAKGYIA
                     AAFHPSGTEFPVVQEILKHVYAGGKRGNMEDKGRIGQQYFNRGVIQGILTAEAIRTAQ
                     AKYGQRPLSGEEMRWGIENLKLDQKRLKELGALNLLQPMQVSCFDHEGGGAVKFQQWD
                     GKQWKVISDWIASDQSIVRPEIEKSAAQYAVEKKLTPRDCPKG"
BASE COUNT          437 a          957 c          878 g          535 t
ORIGIN      
        1 cgccgggggc cgggcactat gggcccatgt ccatccacta ccactacttc acgctggagc
       61 agcgcgacgc gctcgaggcg caaatccgcg cgcgtacttc cagcgatccc gcgctgcggc
      121 tcgcgctcga acgcctgcac caaccggact acggcgtatg catcgaatgc ggcaaggaca
      181 tcgctttcgt gcggctccag gccgatcccg acgccgtgca ctgcgccgac tgcgcgcgcc
      241 tgccggtcaa gcgctagcgg cccggccctc gatcggcgtc cacttccgat tctccggacc
      301 gatgtagttc gcgctcgggc gaatgatctt gccgtcggcg cgctgctcga tgacatgcgc
      361 cgcccagccg gaggtgcgtg aaatcacgaa gagcggcgtg aacatgtccg tcggcacacc
      421 catggcgtgg taggaaaccg cgctgtacca gtcgagattc gggaacatct tcttctcgcg
      481 ccacatcacc ttttccaggc gctcggcgat cgcaaacaac aattgagttg cagaatcttt
      541 ggcgagcctg cgcgctacgc cctttatcac ttcgttgcgc gggtccgaga tcgtgtacac
      601 cgggtggccg aagccgatca cgacctcctt cgcctgcagc cgccgcacga tatcgcgctc
      661 cgcctcatcg ggatccttgt agcgggataa gacggcgaaa gcggcctcgt tggcaccgcc
      721 gtgcttcggg cccttcaacg cgccgatacc tgcgccgatg gcgctgtaca tatcggagcc
      781 ggtgccggcg atgacgcggc aggcgaacgt gctggcgttg aactcatgct cggcgtagag
      841 gatgagcgag gcatgcatcg cccggacatg cgccggtgac ggcggtttgc cgtgcaaaag
      901 gtgcaggaaa tgcccgccga tcgagtcgtc gtccgtctcg acgtccacgc gccgcccctc
      961 ggcgtgaaag tggtaccaat agagcagcat cgagccgagt gaagccatca gccggtcggc
     1021 gatgtgctgc gcgccggcct gcccgtggtc gtccttctcc ggctcgagcg tgccgagcac
     1081 cgaaacgccg gtgcgcaaga catccatcgg gtgcgcggtt tcgggcacgc actcgagcgc
     1141 cgccttgagc atctcgggca gcctgcgcag ccggcacagc ttcttcttgt actcgcccag
     1201 ctcgtctttc gtcggaagat cgccgtgcac gagcaggtag gcgatttcct cgaactccgc
     1261 ccgatcggcg aaatcgagga tgtcgtagcc gcggtagtgg aggtcgttgc cggtgcgccc
     1321 gacgctgcag agcacggtgt ttcccgccgc cacgcccgaa agcgcgaccg atttcttcgg
     1381 cttgggaacc gcttcggtta ggccagccat ctcttcctcc gccggtagtg cttcacgtcg
     1441 cggaagctct tctgcttgcc gctcgaaatg cccagataaa attccttcac gtcctcgttc
     1501 tcggcgagct cggccgaggt gccctcgatg acgacgcgcc cgctctccag gatgtagccg
     1561 aactcggcga acttgagcgc catcaccgcg ttctgctcgg cgaggagaaa gctcaccttc
     1621 tcgcggcggt tgaggttctg cacgatctcg aagatctcct gcacgatctg cggcgcgagc
     1681 cccatcgacg gctcgtcgag caggatcatc ttcggccgcg ccatcagcgc gcggcccacc
     1741 gcggtcatct gctgctcgcc gcctgagatg tagccggcga ggctcgagcg gcggtccttc
     1801 aggcgcggga agtagctgta gacgcggtcg aggtcacgct tgatctcgaa gcggttgctg
     1861 tggcgggtat gcgcgccggt gagcaggttc tcgtcgaccg tcaggtgccc gaagcagtgg
     1921 cgtccctcca tgacctggat cacgccccgg cgcacgagct ccgaggggtt caagcgctcg
     1981 acgcgctcgc cctggaactc gatcgagccc ttggtgactt cgccgcgctc ggcgccgagc
     2041 aggttggaca ccgccttcaa gcaggtggtc ttgccggctc cgttcgcgcc gagcagcgcg
     2101 acgatgccgc cctcgcgcac tgcgagcgac acgcccttca acaccaggat cacgtggtcg
     2161 tagatcacct cgatgttgtt gacgcggagc atcgtcagcc cttcggacag tcgcgtggcg
     2221 tcagcttctt ctcaacggcg tattgcgcgg cggacttctc gatctccggc cgcacgatcg
     2281 actggtcgct cgcgatccag tcgctgatca ccttccattg cttgccgtcc cactgctgga
     2341 acttgacggc tccgccgccc tcgtggtcga agcacgagac ctgcatcggc tgcagcagat
     2401 tgagcgcgcc gagctccttt aggcgcttct ggtcaagctt caggttctcg atgccccagc
     2461 gcatctcttc gccggagagc ggtcgctggc cgtatttcgc ctgcgcggtg cggatcgcct
     2521 cggcggtcag gattccctgg atcacgccgc ggttgaagta ctgctggccg atgcgcccct
     2581 tgtcctccat gttgccgcgc ttgccgccgg catagacgtg cttgaggatc tcctgcacca
     2641 ccgggaattc ggtgccggag ggatggaacg ccgcggcgat gtagcccttg gcggcgtcgc
     2701 ccgcgggcag cacgtcctct tccgccccgc tccaccacac gccgacgatg cgctcggtct
     2761 tgtagccgac cttctgcgct gctttgagcg cggtcggatt catcacg
//
LOCUS       DASTPD010000163         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1720033, whole genome shotgun sequence.
ACCESSION   DASTPD010000163 DASTPD010000000
VERSION     DASTPD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720033"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFR57_05595"
     CDS             complement(<1..579)
                     /locus_tag="VFR57_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0258875.1"
                     /translation="MAKPQVYDASAIQALKGLEPVRRMPGMYTHTVHPLHIVQEAIDN
                     AVDEALAGFGKRITVSVFKDGSVQVEDEGRGIPVDMHPVEKKPAVEVAFTMLHAGGKF
                     SRSGDGVYHISGGLHGVGVSVSNALSKRCEVVVYRNNERHSIVFEGGEVKNKLKSEKI
                     KEKRTGTIVRLWPDPKYFDSPHIPMNELEHLVR"
     gene            complement(633..1091)
                     /locus_tag="VFR57_05600"
     CDS             complement(633..1091)
                     /locus_tag="VFR57_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0258876.1"
                     /translation="MAVVFDCSVTLAWFLKDERTAFTDTAFELLETEECWVPYLWRLE
                     FPNALLFAERKKRLGREERLEILDNAAQLFLRADQALPDIRSLSALAERRGLTVYDAS
                     YLELALRSGFDLITLDRELAAAAVAEGVAAHSPGRSSAAQVRKRYAVEAA"
     gene            complement(1091..1348)
                     /locus_tag="VFR57_05605"
     CDS             complement(1091..1348)
                     /locus_tag="VFR57_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU0258877.1"
                     /translation="MITVGSFEAKTKLAELLDKVEAGETVMITRRGKAVAKLVPAAVD
                     AEERARRRAFIEEVKRKRSAWDRGAKPGTTIADLIKAGRRY"
     gene            complement(1394..2086)
                     /locus_tag="VFR57_05610"
     CDS             complement(1394..2086)
                     /locus_tag="VFR57_05610"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0258878.1"
                     /translation="MVDVEREGPVARIFLNRPEKANALTSRALDELERALRELARDRS
                     LRTVILGGRGRTFCGGADREELSNLTAETAGAFVTRIHEVCQAIRELPVPVVARLHGA
                     VIGAGLEIAAACDLRVAAEGTRFAMPEVRLGIPSVVEAALLPRLMGSGRAAWLVLTGE
                     AIDARRALEWGLIEDLASDLDSRLEFLSKSLLAGDPEALRVQKQLLQLWDEAPLATSV
                     TASIERFARLHK"
     gene            2085..2858
                     /locus_tag="VFR57_05615"
     CDS             2085..2858
                     /locus_tag="VFR57_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEU0258879.1"
                     /translation="MLSSYAREALKKASTATLTTVLFKRGLRNVFIQGIFLLNKDAPR
                     MVGEAYTLRYIPAREDLDQLGAFEGRGHPQREAIEACPSGQVLVMDARRDASAATGGD
                     ILMTRLMVRGVAGVVTDGGLRDSPTIEKLAWPAYCGARSAPLNLVRHHAVESQVPIGC
                     GGVAVYPGDVVVGDAEGVVVIPAKMAEEVAKEAEAQTEFEDWVEAKVKEGRSIFGLYP
                     PNAETKAEFEAFRKKSGSDPDLRLTSQLSYNSPQPGGDP"
BASE COUNT          497 a          964 c          956 g          515 t
ORIGIN      
        1 gcgcaccagg tgctcgagct cgttcatcgg gatgtggggc gagtcgaagt atttcggatc
       61 cggccacagg cgcacgatcg tgccggtgcg cttttccttg atcttttccg acttcagctt
      121 gttcttgact tcgccgcctt cgaaaacgat cgaatgacgt tcattgtttc gataaacaac
      181 aacttcgcag cgcttcgaaa gcgcgttcga caccgagacg ccgacgccgt gcaggccgcc
      241 ggagatgtgg taaacgccgt cccccgagcg cgagaacttg ccgccggcgt gcagcatagt
      301 gaaggcgacc tcgaccgcgg gcttcttctc gaccgggtgc atgtcgaccg ggatgccgcg
      361 gccttcgtcc tcgacctgga ccgagccgtc cttgaacacg ctcacggtga tgcgcttgcc
      421 gaacccggcg agcgcctcgt cgacggcgtt gtcgatcgcc tcctgcacga tgtgcagggg
      481 atgcacggtg tgggtgtaca tccccggcat gcgccggacc ggctcgaggc ccttcagcgc
      541 ctggatggcg gatgcgtcgt agacctgcgg ttttgccatg tatctagttc agtttagctc
      601 gttgtctact aggtatggta gtggaaagcg cttcacgcgg cttccacggc gtagcgcttg
      661 cgcacctggg cggcgctcga gcggcccggc gaatgcgctg ccaccccttc ggcaaccgcc
      721 gcggcggcga gttctcgatc gagcgtgatc aggtcgaagc ccgagcgcag ggcaagctcc
      781 aggtaggagg cgtcataaac cgtgaggccg cggcgctccg ccagcgcgct cagcgagcgg
      841 atatccggca gcgcctggtc ggcgcgcagg aagagctggg cggcattgtc caggatctcc
      901 agccgctcct cgcggccgag gcgtttcttg cgctcggcga acagcagcgc gttcgggaac
      961 tcgaggcgcc agagatacgg cacccagcat tcctcggtct ccagcagctc gaatgcggta
     1021 tcggtgaagg cggtgcgctc gtctttcagg aaccaggcga gagtcaccga gcagtcgaac
     1081 accaccgcca tcagtaacgt cggccggcct tgatcagatc ggcgatcgtc gtgccgggct
     1141 tcgcaccgcg atcccaggcc gagcgcttcc tcttgacctc ctcgatgaac gcacgccgcc
     1201 tcgctcgctc ctccgcgtcg actgcggccg ggacgagctt cgccaccgcc ttgccacggc
     1261 gagtaatcat gacggtttcg cccgcctcga ccttgtcgag cagctcggcg agcttggttt
     1321 ttgcctcgaa cgagcctacg gtgatcattt caaaaaagta ccttccaact agacaaccag
     1381 ttggaaggat actctatttg tgaagccgcg cgaagcgctc gatcgacgcc gtgaccgagg
     1441 tcgcgagcgg cgcctcgtcc cagagctgga gcaactgctt ctgcacccgc agcgcttcgg
     1501 ggtcgcctgc aagcaggctt tttgaaagaa attccaaccg ggaatcgaga tcggacgcca
     1561 ggtcttcgat caacccccac tcgagcgcgc gccgggcgtc gatcgcctcg ccggtaagaa
     1621 cgagccaggc ggcgcgcccg gagcccatga gccgcggcag gagcgcggcc tcgaccaccg
     1681 aggggatgcc gagcctgacc tcgggcatgg cgaacctggt gccctcggcc gcgacgcgca
     1741 ggtcgcacgc ggcggcgatc tcgagccccg cgccgatgac cgcgccgtga agccgcgcca
     1801 ccaccggcac cggcagctcg cggattgcct ggcatacctc atggatacga gtgacaaacg
     1861 cacccgctgt ctccgccgtc agattgctca gttcctcgcg gtcggcgccg ccgcagaagg
     1921 tccggccgcg cccgccgagg atcaccgttc tgagggaccg atcgcgcgca agttcccgca
     1981 acgcgcgctc gagctcgtcg agcgcgcggc tcgtcaaagc gtttgctttt tcgggccggt
     2041 tgaggaagat tctcgcgacc ggcccttcgc gttcgacatc caccatgctt tcatcttatg
     2101 cccgtgaagc tttgaagaag gcttccaccg cgacgctgac gaccgtgctt ttcaagcgcg
     2161 gcttgcgcaa cgtgttcatc caggggattt ttttgttgaa taaggacgct ccgcgcatgg
     2221 tgggcgaggc ctacacgctg cgctacatcc cggcgcgcga ggacctcgac cagctcggcg
     2281 cattcgaggg tcgcggccac ccgcagcgcg aggcgatcga ggcctgcccg agcggccagg
     2341 tgctggtgat ggacgcgcgc cgcgacgcct cggccgcgac cggcggcgac atcctgatga
     2401 cgcggctcat ggtacgcggc gtggcgggcg tggtgaccga tggcgggctg cgcgattcgc
     2461 cgacgatcga aaagctcgcc tggccggcgt actgcggcgc gcgctcggcg ccgctcaacc
     2521 tcgtgcgcca ccacgcggtc gagagccagg tgccgatcgg ctgcggcggt gtcgcggtct
     2581 acccgggcga cgtggtggtc ggcgacgccg agggcgtggt cgtgatcccg gccaagatgg
     2641 ccgaagaggt ggcgaaggaa gccgaagcgc agaccgagtt cgaggactgg gtcgaagcca
     2701 aggtcaagga ggggcgctcg atcttcggcc tttatccgcc caacgccgaa accaaggccg
     2761 agttcgaggc cttccggaaa aaatcggggt cagaccccga tctgcggtta actagtcagt
     2821 tatcatacaa ctcgccccaa ccaggaggag atccgtgaga aagctcgttg ccgtattgct
     2881 gttggccgcg ctcgccctgc cctgcagctt gcccgtttcg gcgcaggaga cg
//
LOCUS       DASTPD010000164         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1107975, whole genome shotgun sequence.
ACCESSION   DASTPD010000164 DASTPD010000000
VERSION     DASTPD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107975"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFR57_05620"
     CDS             <1..709
                     /locus_tag="VFR57_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0258880.1"
                     /translation="GVRAGQTPIIYRGVQVGEVIRVALGPDRSHALVRARLHKAAAPL
                     ASEGALFWIVRPQLSLNQVTGLSTVLSGPEIHVLPGKGAQLQAEFTALEVAPAGIGML
                     GLRLVLRAERPRGVRVNTPVNYRGVEVGLVQRLDLAPNSASADIHILIYSRYAPLVRA
                     GSVFWNTSAVQATGSILKGIELELESLRTLFTGAIEFATPSEKAPRAPAGTVFFLHDK
                     PKDEWLNWSPKIPLAEK"
     gene            706..1395
                     /locus_tag="VFR57_05625"
     CDS             706..1395
                     /locus_tag="VFR57_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0258881.1"
                     /translation="MTFVLVHGAWHGGWCWRLVRDLLEKSGHRVHAPSLTGLGERKHL
                     ARPDIDLDTHIADIVSLLEMEDLSDVVLVGHSYGGMVITGVADRAPERIGRLVYLDAF
                     VPEDGKCRLDYVVPERAARMREEGERVGSVTPPPLSLWGIAKQEHIDFVKPREVRHPY
                     RTMSQKIKLSGKAQDIPRTFIYCSSPATGSFDRFAAKYRGDAAWRFFELKTGHDAMIL
                     MPERVAELLQR"
     gene            complement(1453..1800)
                     /locus_tag="VFR57_05630"
     CDS             complement(1453..1800)
                     /locus_tag="VFR57_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258882.1"
                     /translation="MKRRLLVTAIAAAGLAGCGTEPVTHINYHEMDPSKGMFAESATW
                     EWRRKEPSATSAATPKPAAAPAAAAAAPAAAAPSAAEQEEFKKWRESAGASERQEFEE
                     WRAWQEWKRKNPK"
     gene            complement(1801..>2131)
                     /locus_tag="VFR57_05635"
     CDS             complement(1801..>2131)
                     /locus_tag="VFR57_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258883.1"
                     /translation="WLHTSGINLTASWTQRTQPTLESTPELESEFTYFKVGYKFGQHA
                     IGVDYAQGKDFVTAGDDAKMYGIGYVWNPIRWLELYAAYKIHEFDRGSVSFDDVKIGH
                     IGTRVRF"
BASE COUNT          355 a          703 c          680 g          393 t
ORIGIN      
        1 cggcgtgcgc gcgggtcaga cgccgatcat ctaccgcggc gtgcaggtgg gcgaagtgat
       61 ccgcgtcgcc ctcgggcctg accgaagcca cgcgctcgtg cgcgcacgcc tgcacaaggc
      121 agcggcgccg ctcgcatccg aaggcgcgtt gttctggatc gtgcggccgc agctgagctt
      181 gaaccaggtg accgggcttt cgaccgtgct gagcgggccc gaaatccatg tgttgccggg
      241 aaaaggcgcg cagctccagg ccgagttcac ggccctggag gtagcgccgg cgggcatcgg
      301 catgctggga ttgcgcctcg tgctgcgcgc cgagcgcccc cgcggggtgc gggtcaacac
      361 cccggtgaac tatcgcggcg tcgaggtcgg cctcgtgcag cggctcgacc tcgcgcccaa
      421 ctcggcctcg gcggacatcc acatcctgat ctacagccgc tacgcgccgc tggtgcgcgc
      481 cggctcggtg ttctggaaca cgagcgccgt gcaggcgacc ggcagcatcc tgaagggcat
      541 cgagctcgag ctcgagtcgc tgcgcacgct tttcaccggc gccatcgagt tcgcgacccc
      601 gagcgagaag gcgccgcgtg cgcccgcggg caccgtgttc ttcctgcacg acaagccgaa
      661 ggacgaatgg ttaaactggt cgcctaagat tccgctcgcg gaaaagtgac cttcgttctc
      721 gttcacggcg cgtggcatgg cggctggtgc tggcgtttgg tgcgcgatct tctcgagaag
      781 tccggccatc gcgtccacgc tccctcgttg accggcctcg gcgagcgcaa gcacctcgcc
      841 cgcccggaca tcgacctcga cacccacatc gccgacatcg tctccctgct cgagatggaa
      901 gacttgagcg acgtggtgct cgtcggccac agctacggcg gcatggtgat cacgggcgtt
      961 gctgaccgcg cgcccgagcg catcgggcgc ctcgtctacc ttgacgcttt cgtcccagag
     1021 gacggcaagt gcaggctcga ctacgtggtg cccgagcgtg ccgcgcgtat gcgcgaggag
     1081 ggcgagagag tgggcagcgt caccccgccg ccgctatcgc tctggggaat cgcgaagcag
     1141 gagcacatcg acttcgtgaa gccgcgcgag gtgcgccatc cctacagaac catgtctcaa
     1201 aagataaagc tttccggaaa ggcgcaagac atcccgagga ccttcatcta ctgctcgtcg
     1261 cccgccaccg gctcgttcga ccgcttcgcc gcgaaatatc gcggagatgc cgcctggaga
     1321 ttctttgagt tgaaaaccgg acacgacgcg atgatcctca tgcccgagcg cgtggccgag
     1381 ctcctgcagc gctagaaaca aaaaggggtc gaagcgattt gctccgaccc ctcaagtact
     1441 actccctgag cgttatttcg ggttcttgcg cttccactcc tgccaggcgc gccattcttc
     1501 gaactcctgg cgctccgacg ctcccgcaga ctcccgccac tttttgaact cttcttgctc
     1561 ggccgcgctt ggcgctgcgg ctgctggtgc cgccgcagct gctgccggtg cggcagcggg
     1621 cttcggcgtc gcagcggatg tcgcggatgg ctccttgcgg cgccattccc acgtggccga
     1681 ctcggcaaac atgcctttcg aagggtccat ctcgtggtaa ttgatatgcg tgaccggctc
     1741 ggtgccgcag ccggcgaggc cggccgcggc tatggcggta actagaagcc tgcgtttcac
     1801 ttagaacctt acgcgggtgc cgatgtgacc gatcttgacg tcatcgaaac tgacgctacc
     1861 ccgatcaaac tcgtgaatct tatacgcggc atagagctcg agccagcgga tggggttcca
     1921 cacgtagcca atgccgtaca tcttggcatc gtcacccgca gtgacgaagt ccttgccttg
     1981 cgcgtagtcg accccgatgg cgtgctggcc gaacttgtag cccaccttga agtacgtgaa
     2041 ctccgactcg agctcgggtg tgctctctag ggtcggttgt gtgcgctggg tccaggaagc
     2101 ggtcaggttg atacccgagg tatgcagcca g
//
LOCUS       DASTPD010000165         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_291391, whole genome shotgun sequence.
ACCESSION   DASTPD010000165 DASTPD010000000
VERSION     DASTPD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291391"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VFR57_05640"
     CDS             complement(<1..1475)
                     /locus_tag="VFR57_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HEU0258884.1"
                     /translation="MDLRLPVVLACLVACCAAWAQALDVAWPREVKAADGTVITVYQP
                     QVESWADNNLSGRAAVAVKRPNEKEPHYGVIELAARTAVDKSADLVTLSSVRITKSSF
                     PGASQEESQKYLAALRASVTKQSWPVSAQALQANLAVTQARSKQKTVEVKNDPPQIIF
                     RNVPSMLVIVDGEPALRPAKDVPALWRVINTGALILQDPTTKTHYLWAVGRWFEAKDV
                     AGEWRPAASPPASVEKARAALGKQFDPLEGKTADGKSVFEAGTVPQIIVATKPTELLQ
                     SKGEPQLLPIPGTQLLYMANSPNDIFLELGPQTYFVLISGRWFSAKALTGPWAYVPGK
                     SLPVDFARIPPNHQMADVLVSVPGTPQAHEAAIANDIPQTASVQRDVQPTPPVFDGGK
                     PQWKAVDKTPLDYAPNTAAPVIRVDPKTYYMVQNGVWFAATAPEGPYVVATTVPSVIY
                     TIPASSPIHYVTYVRVYGSTPTTVVVGYTPGYYGTVTTVDNV"
     gene            complement(1525..1890)
                     /locus_tag="VFR57_05645"
     CDS             complement(1525..1890)
                     /locus_tag="VFR57_05645"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEU0258885.1"
                     /translation="MKFVFAILIAVAAAPAAAQNWVALLKNTPAERFDEEDLRLFMDN
                     ARKVLAEGKVNETSSWENPKTRARGDVTLLRTFESKGRPCKEVRVHNEAQGRKNTSKL
                     ALCQVEGKWRLLSASQLKK"
     gene            complement(1902..>3137)
                     /locus_tag="VFR57_05650"
     CDS             complement(1902..>3137)
                     /locus_tag="VFR57_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU0258886.1"
                     /translation="ERDEGNGGDNSRSERPGETPREFRGDNVRSIEPIPSGKQRWRLA
                     AVLALTAAEALAADPTIFNREMGVDPSKELAEARFFEDVVVAPIPISNPTVGTGLAVV
                     VMPFYHLGAESPLSNTAIAGGVTSSGSWGVGAAQSTRLRGDHIRIDGFLGYVDLRYRF
                     YGTGASAGESGVSVPILQKGFAFAPELLFKTARGMFFGLRYRGLRVETAVDGDPGILP
                     PEVAVALPNSITIVSSGIGPRATFDTRDNDMNPSSGVLIDVRANFADQSLGSDLDYQT
                     YDLGANYYRRTGPGVLALRGYLCQASDQTPLFDLCLFGAGSDLRGYEVGRYRDQAMIA
                     AQAEYRFPLGGRFGGVVFGGWGKVAPSFDEMDEQLVLPSVGFGLRWLAAEKARVNLSV
                     DIARGRDSTSLYVYVKESF"
BASE COUNT          518 a         1029 c         1024 g          566 t
ORIGIN      
        1 acgttatcca cggtcgtcac ggtgccgtag tagccgggcg tgtagccgac gaccaccgtg
       61 gtcggcgtgc tgccgtagac gcgcacgtac gttacatagt gaatcggcga gctggccggg
      121 atggtgtaga tcaccgatgg caccgtggtc gcgacgacgt acggaccctc cggcgcggtg
      181 gcggcgaacc agacaccgtt ctgcaccatg tagtaggtct tcgggtcgac gcggatcacg
      241 ggcgccgcag tgttgggtgc gtagtcgagg ggcgtcttgt cgaccgcctt ccattgcggc
      301 ttgccgccgt cgaagaccgg gggcgtgggc tgcacgtcac gctgcacgct cgccgtctgc
      361 gggatatcgt tcgcgatggc cgcctcgtgg gcctggggcg tgcccggcac cgagacgagc
      421 acgtcggcca tttggtgatt cggcgggatg cgggcaaagt ccacgggcag cgacttgccg
      481 ggcacatagg cccacggtcc ggtgagcgct ttcgcgctga accagcgacc cgagatcagc
      541 acgaagtagg tctgcgggcc gagctcgagg aagatgtcgt tcggcgagtt cgccatgtag
      601 agaagctgcg tgcccgggat cggcaggagc tgcggctcgc ctttcgattg caggagctcg
      661 gtcggcttgg tcgcgacgat gatctgcggc acggttcccg cctcgaaaac gctcttgcca
      721 tcggcggtct tgccttccag cggatcgaat tgcttgccaa gcgccgcgcg ggctttctcg
      781 accgatgcgg gcggcgaggc ggcgggcctc cattcgcccg cgacgtcctt cgcctcgaac
      841 cagcgcccga ccgcccagag atagtgcgtt ttcgtcgtcg ggtcctgcag gatcaacgcg
      901 ccggtattga tcacgcgcca gagcgccgga acgtccttcg ccgggcgcag cgctggctcg
      961 ccgtccacga tcacaagcat cgagggcacg ttgcggaaga tgatttgcgg cgggtcgttc
     1021 ttcacctcca ccgtcttctg cttcgagcgg gcctgcgtca ccgcgaggtt cgcctgcagc
     1081 gcttgcgccg acaccggcca ggattgcttc gtcaccgacg cacgtagcgc ggcgagatat
     1141 ttctgcgatt cctcttggct tgcgcccggg aagcttgact tggtgatgcg cacggaacta
     1201 agggttacga gatcggccga cttgtcgacc gcggtgcgtg cggcgagctc gatcacgccg
     1261 tagtgcggct ccttctcgtt cgggcgcttc accgcaaccg ccgcccggcc cgacaagttg
     1321 ttgtccgccc agctttcgac ctgcggttgg tagacagtaa tcacggtccc gtcggcggcc
     1381 ttcacctcgc gcggccaggc gacgtcgagc gcctgcgccc aggccgcgca acacgcgacc
     1441 aggcacgcaa gtacgactgg cagccgcaag tccattcccc gaaccatagt gaagcctctt
     1501 cccgcgcgat atccgacgtt ggtattactt cttgagctgc gaggcgctca gcaggcgcca
     1561 cttgccttcg acctggcaca gggcgagctt gctcgtgttc ttgcggccct gcgcctcgtt
     1621 gtgcacgcgc acctccttgc acggccggcc cttggactcg aaggtgcgca gaagcgtgac
     1681 gtcgccgcgc gcgcgggttt tcggattctc ccagctcgag gtctcgttca ccttgccctc
     1741 ggccagcacc ttgcgcgcgt tgtccatgaa caggcgcagg tcctcctcgt cgaagcgctc
     1801 ggcgggcgtg ttcttgagca gcgcgaccca gttctgcgcc gcggccggag ccgcggccac
     1861 cgcgatcagg atggcgaaaa cgaatttcat gacgccccct cctagaagga ctccttaacg
     1921 taaacataaa ggctcgtgct gtcacgtccg cgcgcgatat cgacgctcag gttgacgcgc
     1981 gccttttccg cggcgagcca gcgcagtccg aacccgacgc tcggcagaac gagctgctca
     2041 tccatctcgt cgaacgatgg cgccaccttg ccccagccgc cgaagactac gccgccgaag
     2101 cggccgccca gcggaaagcg gtattcggcc tgcgccgcga tcatcgcctg gtcgcggtag
     2161 cggccgacct cgtacccccg caggtcgctg cccgcgccga aaaggcatag gtcgaaaagc
     2221 ggcgtttgat cggatgcctg gcaaaggtaa ccgcgcagcg caagcacgcc tgggccagtg
     2281 cggcggtagt agtttgcgcc gaggtcgtac gtttggtaat cgaggtcgct gccgagggac
     2341 tggtcggcga aattcgcgcg cacatcgatc agcacgccgg aggacggatt catgtcgttg
     2401 tcgcgcgtat cgaatgttgc ccgggggccg attcccgagc tgacgatggt gatggagttg
     2461 ggaagggcta ctgcaacttc gggcggcaat atacccggat cgccgtcgac cgccgtttcg
     2521 actctcaagc cccgatagcg caagccgaag aacatgccgc gcgccgtctt gaagagaagc
     2581 tcaggcgcga aagcgaaccc cttttgcagg atggggaccg agacgccgct ttcgccggcg
     2641 ctcgcgccgg tgccatagaa gcggtagcgc agatcgacat agcccagaaa gccatcgatc
     2701 ctgatatggt cgccgcgcag gcgggttgac tgagccgcgc ccacgcccca gcttccgctc
     2761 gaggtgacgc cgccagcgat cgcggtgttc gaaagcggcg actcggcacc caggtgataa
     2821 aacggcatca ccaccaccgc gagcccggtg ccgacggtcg ggttcgagat cggaatcggc
     2881 gccacgacta catcctcgaa gaagcgcgcc tcggcgagct ccttcgacgg gtcgacgccc
     2941 atttccctgt tgaaaatcgt ggggtcggcg gcgagcgctt cggccgcagt caaggcaagc
     3001 actgcggcaa gccgccaccg ttgtttgccg gacggaattg gttctatgct gcggacatta
     3061 tcgcctcgaa actcacgcgg cgtctcgcct ggacgctcgg agcgactgtt gtcgccgccg
     3121 ttaccctcat cgcgctc
//
LOCUS       DASTPD010000166         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_700866, whole genome shotgun sequence.
ACCESSION   DASTPD010000166 DASTPD010000000
VERSION     DASTPD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700866"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VFR57_05655"
     CDS             <1..161
                     /locus_tag="VFR57_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258887.1"
                     /translation="GSLRGPFLAALLIGVADTACKYWIPEFGAFLVYAATIGILLWRP
                     QGLFGVRA"
     gene            166..1128
                     /locus_tag="VFR57_05660"
     CDS             166..1128
                     /locus_tag="VFR57_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0258888.1"
                     /translation="MSGYSADPRIRWTEWLPWLAAIAFFFLMPGYLSLGARILIFILF
                     ALSLDLILGYAGIVTLGHSAFFGLGAYTAGALGAHTGATDPLVQLVAAAALAALLGLA
                     TGALILRTRALTLLMLTLAITAILLEIANKWTPMTGGADGLSGVKVGEILGLFRFDLY
                     GRTAFFYCLVMLFIGWWVVRRIVYSPYGAMLTGIRENAARMQAIGAPVYWRLVLIYTI
                     SAILAGIAGALLTQVNQFVGLNVLGLEPSGDILVMLILGGVGRLYGAFVGPVVYLIAQ
                     DYLAKQYPEYWYFGIGVLLIVVVLFARGGILGILDVLLGNKKAV"
     gene            1106..1846
                     /locus_tag="VFR57_05665"
     CDS             1106..1846
                     /locus_tag="VFR57_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258889.1"
                     /translation="METRKLCKSFGALTVADHIDFRLERGARHALIGPNGAGKTTFVN
                     MLTGRLAPSSGQILLVDEDVTHMPQSARVRRGLGRTFQITTLFRNLPVLDNVALGIAE
                     RDGTAMRMWRPASAHRETIEEARSLLAALDLADDAATRVADLPYGKQRLVEIAIALGL
                     RPKVLLLDEPAAGVPSHEAEKILTVLETLPSDIAILIIEHDMDLVFRFAQRITVLVQG
                     TVLVEGSPADIARDPRVHQVYLGEQSRA"
     gene            1839..2531
                     /locus_tag="VFR57_05670"
     CDS             1839..2531
                     /locus_tag="VFR57_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0258890.1"
                     /translation="MLSLSKVRAGYGDTVVLEDVDLELPERGALAVLGRNGVGKTTLL
                     ATIMGHTTFHSGTITFNGNPIAQRPVYERSKLGIGYVPQEREIFPSLSLEENLLVAAR
                     PGRWNLATVYKLFPRLQERRAAGGNQLSGGEQQMLAIGRALMGNPSLLLMDEPLEGLA
                     PIIVEALLISLKKLVSEDSLAVVLVEQHAKLALQVTASALVLNRGRVSYSGPSDELLA
                     DPQRLTSLVLAQ"
     gene            complement(2473..>3103)
                     /locus_tag="VFR57_05675"
     CDS             complement(2473..>3103)
                     /locus_tag="VFR57_05675"
                     /inference="COORDINATES: protein motif:HMM:NF023302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HEU0258891.1"
                     /translation="DVRGWAEIALVAFAATVASCVFAAATMAWFRTRCRWWEVALLLL
                     ATFALFRPDYFMDHMYAPYTEVPAKEVFRVAGELAEDDRLVLVIEGTNVEGDDVRKTV
                     AVQLGKPGDGRKRIAEAGLTISALGPEVRVSAVKFGSRAKKSGFEQGFKVAAVKVPSD
                     RPSEHWVYIPALALVALVWFLQRARERLPGATGREPATSGAAGRRGARR"
BASE COUNT          490 a         1049 c          999 g          565 t
ORIGIN      
        1 tgggcagcct gcgcggcccg tttctcgcag cgttgcttat cggcgtggcg gacacggcgt
       61 gcaagtactg gattccagag tttggagcgt tcctcgtcta tgcggccacc ataggaattc
      121 ttttgtggag accgcaaggc ttgttcggag tccgggcatg actcgatgag cggctacagc
      181 gcagacccga ggatccgctg gacggagtgg ctgccgtggc tcgccgccat cgcgttcttc
      241 ttcctgatgc ctggctacct ctcgctcggc gcgcggattc tgatcttcat cctgttcgcg
      301 ctgtcgctcg acctgatcct cggctacgcc ggaatcgtca cgctcggtca cagcgcgttc
      361 tttggactgg gcgcttatac cgcaggggcg ctcggcgcgc ataccggcgc gaccgatccg
      421 ctcgtgcagc tcgtcgccgc ggcggcgctc gcggccctgc tcggcctagc caccggtgcc
      481 ttgatcctgc gcacccgcgc gctcacgctg ctcatgctca cgctcgcgat cacggcgatc
      541 ctcctcgaga tcgccaacaa gtggaccccc atgacgggcg gcgccgacgg attgtccggg
      601 gtgaaagtgg gcgagatcct gggcctgttc cgcttcgatc tttatggcag gactgccttt
      661 ttctactgcc tcgtgatgct gtttatcgga tggtgggtgg tgcgccggat cgtgtattcc
      721 ccatacgggg cgatgctcac cggcatccgc gaaaacgcgg cgcgcatgca ggcgatcggc
      781 gcgccggtgt actggcggct cgtgctcatc tacaccatct cggccatcct ggcgggaatc
      841 gccggtgcgc tgctcacgca ggtgaaccag ttcgtcgggc tgaacgtcct cggcctcgag
      901 ccctcgggcg acatcctggt catgctgatc ctcggcggcg tcggccgcct ctatggcgcg
      961 ttcgtcggac cggtcgttta cctgatcgcg caggactacc tcgccaagca gtatcccgag
     1021 tattggtact tcggcatcgg ggtcctgctg atcgtcgtgg tgcttttcgc gcggggcgga
     1081 atcctcggaa tcctcgatgt actccttgga aacaagaaag ctgtgtaaga gcttcggcgc
     1141 gctgaccgtc gccgaccata tcgacttccg gctcgagcgg ggagcgcgcc atgcgctcat
     1201 cgggccaaac ggcgcgggca agacgacgtt cgtcaacatg ctcacgggca ggctcgcgcc
     1261 ctcctccggg cagatcctgc tcgtcgacga ggacgtgacg cacatgccgc aatcggcacg
     1321 cgtgcgccgc ggcctcgggc gcacgttcca gatcaccacg cttttccgca acctgcctgt
     1381 gctcgacaac gtggcgctcg ggatcgcgga gcgcgacggc acggcgatgc gcatgtggcg
     1441 cccggcttcg gctcaccggg agacgatcga agaggcgagg agcctgcttg cagcgctcga
     1501 ccttgccgac gacgccgcga cgcgcgtcgc cgacctgccc tacgggaagc aacgcctggt
     1561 cgagattgcg atcgcgctcg ggctgcggcc caaggtgctg ctgctcgacg agccggcggc
     1621 cggtgtgcct tcgcatgaag cagaaaaaat acttacggtt cttgaaacct taccctccga
     1681 cattgccatc ctgatcatcg agcacgacat ggatctcgtg ttccgcttcg cgcagcgcat
     1741 cacggtgctc gtgcagggca cggtgctggt cgaaggatct ccggccgaca tcgcgcgcga
     1801 tcctcgggtg caccaggtgt atctcggaga gcagagtcgt gcttagcctg tcaaaggtac
     1861 gagcgggtta cggcgacacg gtcgtgctcg aggacgtcga tctcgagctt cccgagcgcg
     1921 gcgcgctggc ggtgctcggc cgcaacggcg tcggcaaaac gacgctgctc gcgaccatta
     1981 tgggccacac cacgttccac tccggcacga taaccttcaa cggcaatccg atcgcgcagc
     2041 gccccgtcta cgaaaggtca aagctgggga tcggctacgt gccccaggag cgcgagatct
     2101 ttccgtcgct ctcgctcgaa gaaaacctgc tcgttgccgc acgcccagga cgctggaacc
     2161 tcgccacggt atacaagctc ttccccaggc tgcaggaacg aagagcggcc ggcggcaatc
     2221 agctctccgg tggcgagcag cagatgcttg ccatcggccg cgcgctgatg ggaaaccctt
     2281 ctctgctcct catggacgag ccgctcgagg ggctcgctcc catcatcgtc gaggcactct
     2341 tgatttctct caagaagctc gtttcagagg attccctggc ggtggtgctg gtcgagcagc
     2401 acgccaagct cgcgctccag gtgacggcga gcgcgctggt cctgaaccgt ggccgcgtga
     2461 gctactcggg gcctagcgac gagctcctcg ccgacccgca gcgcctgacg tcgctggttc
     2521 tcgcccagta gcgcctggca ggcgttcccg ggcgcgctgc aggaaccaga ccagcgcgac
     2581 gagcgcgagc gccgggatgt agacccagtg ctcggagggc cggtcgctcg gcaccttgac
     2641 cgccgcgacc ttgaagccct gctcgaagcc ggatttcttc gcgcggctgc cgaacttcac
     2701 cgcgctcacc cgcacttccg gcccgagcgc cgagatggtg aggccggcct cggcgatgcg
     2761 cttgcgcccg tcgccgggct tgccgagctg caccgcgacg gtcttcctca cgtcgtcgcc
     2821 ctcgacgttg gtgccctcga tcaccagcac caggcggtcg tcttccgcca gctcgccggc
     2881 cacgcggaaa acctcctttg cgggaacctc cgtgtagggc gcgtacatgt ggtccatgaa
     2941 gtagtccggg cggaacagcg cgaaggtagc gagcaacagc agcgccacct cccaccagcg
     3001 gcagcgcgtg cggaaccacg ccatggtggc ggcggcgaac acgcacgagg cgacggtggc
     3061 ggcgaacgcg accagcgcga tctcggccca gccgcgcacg tcg
//
LOCUS       DASTPD010000167         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_86999, whole genome shotgun sequence.
ACCESSION   DASTPD010000167 DASTPD010000000
VERSION     DASTPD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86999"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFR57_05680"
     CDS             <1..716
                     /locus_tag="VFR57_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0258892.1"
                     /translation="FIAVLLGGGAWLDFQPLSTLPAVLVTLIAGTVAVALVLPARGAH
                     AATPGSALRFTPEVCALLAAAFCNAVAHGALYAFLSLHLEALGYSATAIGMLWTLGVL
                     AEIVVFLYLPQLFRRFALSAIMAASAACGIVRFLAIGWAAGEVGIVLLAQVLHAATFG
                     SFHAASVAAVHRVIPESAHARGQTLFSGVTYGAGATAGLIISGWAWELGGAPLAFSAA
                     ALAAGAGVPFALRLKRAGL"
     gene            732..1562
                     /locus_tag="VFR57_05685"
     CDS             732..1562
                     /locus_tag="VFR57_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0258893.1"
                     /translation="MVRILLIAWAGAAVLISGCQSVETTQTGVVGVDREQRMAVSAEE
                     VNAGAVKAYAQMMAEAKQKGVLDRDPAMVKRVQDITRRLIPQTTAFRPDAAKWPWEVH
                     VITLDEVNAWCMPAGKMAIYTGLIQKLNATDDEIAAVMGHEIAHALREHARERISRQM
                     GTQAAIGIAGALLGIGELGQSVAGAVADVTLNLPNSRLHETESDRIGVELAARAGFDP
                     RASVTLWEKMAKVAGGSQPPKWLSTHPPHQDRISDLRAYSDKVMPLYTAARSSAAAGK
                     "
     gene            1594..3006
                     /gene="leuC"
                     /locus_tag="VFR57_05690"
     CDS             1594..3006
                     /gene="leuC"
                     /locus_tag="VFR57_05690"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0258894.1"
                     /translation="MGKTLYDKLWESHVVHEEDDSTALLYIDRHLVHEVTSAQAYEGL
                     RAAGRKPWRTESVVATADHNTPTTDWDQGIEGVRDPMARLQIETLDSNMKEFGAKVYF
                     PFLDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQ
                     CLVTRKMKNMRVNVEGPLARGVSAKDVVLAIIGRIGIAGGTGYTIEYAGSTIRALSME
                     GRMTVSNMTIEAGARAGMVAFDDITLEYVKGRRFAPQGGDWDRAVAYWRTLKSDLDAK
                     FDREVTLDAREICPHVTWGTSPEMVVTVEDGVPDPDKEKDSVRREGMERALQYMGLKP
                     RTPITEIRIDKVFIGSCTNSRIEDLRAAAAVVKGRKKASNVRLALVVPGSGLVKQQAE
                     REGLDRIFRDAGFEWREPGCSMCLGMNPDRLEPGERCASTSNRNFEGRQGTGGRTHLV
                     SPPMAAAAAIAGHFVDIRKW"
     gene            3000..3593
                     /gene="leuD"
                     /locus_tag="VFR57_05695"
     CDS             3000..3593
                     /gene="leuD"
                     /locus_tag="VFR57_05695"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0258895.1"
                     /translation="MVKFTVLNGLVAPLDRANVDTDQIIPKQFLSSIKRTGFGQGLFA
                     EWRKDPQFVLNQPRYKGATILLARKNFGCGSSREHAPWALMDYGFRCVIAPSFADIFF
                     TNSFKNGFLPLVLSEAEADSLFHDCAAFPGFRLVVDLERQTVSTPDNGKTFRFEIEPF
                     RKYCLLNGLDEIGLTLRRVDKIRAFEAQRKAQYPWYF"
     gene            3595..4460
                     /gene="leuB"
                     /locus_tag="VFR57_05700"
                     /pseudo
     CDS             3595..4460
                     /gene="leuB"
                     /locus_tag="VFR57_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
BASE COUNT          777 a         1452 c         1484 g          747 t
ORIGIN      
        1 ggttcatcgc ggtgctgctc gggggcggcg cctggctcga cttccagccg ctttcaacgc
       61 tgcccgccgt gttggtcacg ctcatcgcgg ggacggttgc cgtcgcgctc gttttgcccg
      121 cccgaggagc gcacgcggcg actccaggct cggcgctgcg cttcactccg gaagtatgcg
      181 cgctccttgc cgccgccttc tgcaacgcgg tcgcgcatgg cgcgctctac gcctttctca
      241 gcctgcacct cgaggcgctt ggctacagcg cgacggcaat cggcatgctt tggacgctcg
      301 gcgtgctcgc agagatcgtg gttttcctct acctgccgca gcttttccgc cgctttgcgc
      361 tgtcggcgat catggcggcc agcgcggcct gcggcatcgt acgtttcctc gcgataggct
      421 gggcggcggg cgaggtgggg atagtcctgt tggcgcaggt tctgcacgcg gcgaccttcg
      481 gatcgtttca tgcggcaagt gtcgcggcag tgcaccgcgt gattccggaa agcgcgcatg
      541 cgcgcggcca gacgttgttt tccggcgtca cctacggcgc cggtgccact gccgggctga
      601 tcatttccgg ctgggcctgg gagctgggag gtgctcctct cgcgttttcc gctgcggcgc
      661 ttgctgcggg ggcgggagtg ccgtttgccc tacgcttgaa acgagcgggg ctttgaactg
      721 tcagagacgt catggttcgc atactcctta ttgcatgggc cggggcggca gtgctgattt
      781 ccggctgtca gagcgtggag accacccaga caggcgtggt cggtgtagac cgcgagcagc
      841 gcatggcggt atcggcggag gaggtgaatg ccggcgccgt caaggcgtac gcgcagatga
      901 tggccgaggc gaaacagaaa ggcgtcctcg accgggaccc cgcaatggtg aagcgtgtgc
      961 aggacatcac gcggcggctc attccgcaaa ccactgcctt ccgtcctgac gccgccaagt
     1021 ggccctggga agtccatgta atcaccctgg acgaggtgaa tgcctggtgc atgccggccg
     1081 gcaagatggc catctacacc ggcctgattc agaagctgaa cgcgaccgat gacgagatcg
     1141 ccgcggtcat ggggcacgaa atcgcgcatg ccctgcgcga gcacgcgcgc gagcgtattt
     1201 cccgccagat gggaacgcag gcagcgatcg gcatcgccgg cgcgctgctc ggcatcggcg
     1261 agctcggcca gagcgtcgcc ggagcggtcg ccgacgttac gctcaacttg ccgaactcgc
     1321 gcctccacga gaccgaatcc gaccgcatcg gcgtcgagct ggcggcgcgc gccggcttcg
     1381 acccgcgcgc ctcggtgacg ctgtgggaga agatggccaa ggtggccggc ggctcgcagc
     1441 cgccgaagtg gctctcgacg cacccgccgc accaggatcg catcagcgac ctgcgcgctt
     1501 attccgacaa agtgatgccg ctttacacag cggccaggag cagcgcagca gccggcaaat
     1561 aacttaaaat cccatgcttt cgcagcagaa agcatgggta agacgctcta cgacaagctt
     1621 tgggagagcc acgtcgtcca cgaggaggac gacagcaccg cgctcctgta catcgaccgc
     1681 cacctcgtcc atgaagtgac gagcgcgcag gcgtacgagg gcctgcgcgc cgcagggcgc
     1741 aagccctggc gaaccgagtc ggtggtggcc accgctgacc acaacacgcc gaccaccgac
     1801 tgggaccagg gcatcgaagg cgtgcgcgat cccatggcgc gcctgcagat cgagacgctg
     1861 gattccaaca tgaaggagtt cggcgccaag gtctactttc cgtttctcga caagcgccag
     1921 ggcatcgtcc acgtcattgg cccggagcag ggcgcgacgc tccccggcat gaccgtagtg
     1981 tgcggcgact cacacaccag cacgcacggc gcgttcgcct gtctcgcgca cggcatcggc
     2041 acctccgagg ttgagcacgt gctcgccacg cagtgcctcg tcacccgcaa gatgaagaac
     2101 atgcgcgtga acgtcgaagg gcctcttgcc cgcggcgtca gcgccaagga cgtggtgctc
     2161 gcgatcatcg gccgcatcgg catcgcggga ggcaccggct atacgatcga gtacgccggc
     2221 agcaccattc gcgcgctgtc gatggaaggc cgcatgacgg tgagcaacat gacgatcgag
     2281 gccggcgcgc gcgcgggcat ggtcgcgttc gacgacatca cgctcgagta cgtgaaaggg
     2341 cgcaggttcg cgccgcaggg cggcgactgg gaccgcgcgg tggcgtactg gcgcacgctg
     2401 aagagcgacc tcgacgcgaa gttcgaccgc gaggtcacgc tcgacgcgcg cgagatctgc
     2461 ccgcacgtca cctggggcac ttctcccgag atggtggtca cggtcgagga cggcgttccc
     2521 gatccggaca aggagaagga cagcgtgcgc cgcgaaggca tggagcgcgc gctgcagtac
     2581 atgggcctca agccgcgcac gccgatcacg gagatcagga tcgacaaggt cttcatcggc
     2641 tcgtgcacga attcgcgcat cgaggacctg cgggccgccg ctgcggtcgt caaaggaaga
     2701 aaaaaggcct cgaacgtgcg cctcgcgctg gtggttccgg gctcgggcct ggtaaagcag
     2761 caggccgagc gtgaagggct ggaccggatc ttccgcgacg ccggcttcga gtggcgcgag
     2821 cccggctgct cgatgtgcct cggtatgaac ccggatcgac tggagcccgg cgagcgttgc
     2881 gcctccactt ccaatcgcaa cttcgaaggt cggcagggca cgggcggccg cacccacctg
     2941 gtcagtcctc ccatggctgc ggccgcggca atcgcgggcc atttcgtgga catcagaaaa
     3001 tggtgaagtt caccgttctg aacggccttg tcgccccgct cgaccgcgcg aacgtcgaca
     3061 ccgaccagat catccccaag cagttcctgt cgtcgatcaa gcgcaccggc ttcggccagg
     3121 gtctgttcgc cgaatggcgc aaggacccgc agttcgtgct caaccagccg cgctacaagg
     3181 gcgcgacgat cctgcttgcg cgcaagaact tcggctgcgg ctcgtcgcgc gagcacgcgc
     3241 cgtgggcgct catggactac ggcttccgct gcgtgatcgc gccgtcattt gccgacatct
     3301 tcttcaccaa ctcgttcaag aatggtttcc tccccttggt gctgagcgag gccgaagccg
     3361 acagcctgtt ccacgattgc gccgcttttc ccgggtttcg cctcgtagtc gacctcgagc
     3421 gccaaacggt atcgaccccg gacaacggga aaaccttccg cttcgagatc gagccattcc
     3481 gcaagtactg cctgctgaac ggccttgacg agatcgggct tacgctgcgc cgcgtggaca
     3541 agattcgcgc gttcgaagcg cagcgcaaag cccagtatcc ctggtacttc taggatgaag
     3601 atcgccgtgc tgcccggtga cggcatcggg ccggagatcg ttgcgcaagc tgtccgggtg
     3661 ctgcaggcgc tcgagttgcc cctgcagatg gaaactgcgc cggtcggggg tgcaggctac
     3721 gaggccgcgg gcgacccgct tcccgcaatc acattgagta ttgccaagga agccggtgcg
     3781 gtactgttcg gcgcggtggg cgacgcgcgc tatgaccagc tcgagcgctc caagcgtccc
     3841 gagcaggcga ttcttgggct gcgcaaggcg ctcgggctct tcgccaacct gcgcccggcg
     3901 cgcgtgcatc ccgagctcgc cgcggcgtcg gcgctcaagg cagaggtggt ctccgggctc
     3961 gacatcctca tcgtgcgcga gctcaccggg gacatctact tcggcgagcc gaagggaata
     4021 agaaagctcg atggcgtgcg cgagggcttc gacaccatgc gctacagcga gcccgagatc
     4081 cggcgcatcg cgcgcgtcgc cttcgaggcg gcccgaaggc gggcgaagcg cgtttgctcg
     4141 gtcgataagg cgaacgtgct ggagacttcg cagctgtggc gggaagtggt ttctcaagaa
     4201 gcaagaaact acgccgacgt cgagctgacc cacatgtacg tggacaactg tgcgatgtag
     4261 ctcgtgcgca accccaagca gttcgacgtc atcgtgaccg gcaacatgtt cggcgacatc
     4321 ctttccgacg aggcgtcgat gctcaccgga tcgatcggca tgctgccctc ggcgtctctc
     4381 aacgagcgcg gcaaggggct gtacgagccg atccacggca ccgcgcccga catcgcgggc
     4441 aaagggctcg caaacccgct
//
LOCUS       DASTPD010000168         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1517361, whole genome shotgun sequence.
ACCESSION   DASTPD010000168 DASTPD010000000
VERSION     DASTPD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517361"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFR57_05705"
     CDS             complement(<1..462)
                     /locus_tag="VFR57_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0258896.1"
                     /translation="MSDLCALTASELVDAFRKRTLSPVEVTAAVLKRIEALNPVLNAF
                     SLVSENALNEAKASEARWLAGQPKGLLDGVPVSIKDLILTRGWPTRRGSKTIDPKGPW
                     NDDAPATARLREHGAVLLGKTTTPEFGWKGVTDSPLTGITRNPWNKAKTPGG"
     gene            complement(459..1172)
                     /locus_tag="VFR57_05710"
     CDS             complement(459..1172)
                     /locus_tag="VFR57_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0258897.1"
                     /translation="MNEELLDDLVAANRILAEHGVIDAYGHVSIRSPDDPKRFFLACA
                     IAPETVQRGDILEYDLDAQALDARGRDSVNERFIHSEIYRARPDVIAVVHNHSPSVVP
                     FSVTGVKMRALFHMASFIGDGLPNFEIRKAKKGSDLLVRTPQLGAALAKTLGKKPAAL
                     MRGHGSVVIGENLQRAVGRSIYLEMSARLQMQALLLSKRITFLDAAEVKASAPVQDYK
                     RAWPLWRDKALARARADRR"
     gene            complement(1235..2185)
                     /locus_tag="VFR57_05715"
     CDS             complement(1235..2185)
                     /locus_tag="VFR57_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258898.1"
                     /translation="MRGIVALAAAFAFGVACAQDFPSRAVHIIVPYTPGTGADILSRL
                     LGARLAERWKIGVVTENKPGATGNIGAAFVAKSPPDGYTLLFVATSFGTTPALGKVPF
                     DPVKGFAPVALVATSGLVVVVNPQLPARSMRELIELAKREPGKLYYSSPGNGGPQHLA
                     MELLKLELGMDIVHVPYKTAAGAVGDVVGGHVQATIAAVQTAQPQVLSGQLRALAVLS
                     TKRSQAFSAVPTLKEQGLPELEIETWYGAFAPAGTPAGVVAKINADLNSLLADPGLSE
                     LLSRQGMSVVGGPPERLSELLKRELERWARVVSAAGIKAD"
     gene            2207..3772
                     /locus_tag="VFR57_05720"
     CDS             2207..3772
                     /locus_tag="VFR57_05720"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0258899.1"
                     /translation="MDTDVLIVGAGPVGLVLAIDLGRRGVRCTLIERKPEPQFLPKME
                     RCNARTMEIFRRMGIATLVRAAGLRADVPMDVYIICAMNKPPLLHHPYPSVAEARREI
                     DACTDGSLPLEPYQLISQYTLEPLLTSVAENLPSVTVRYGCEFLSFSQDAMGVVTQTS
                     SDTIASKYLVGCDGGASTVRQQLGIALSGEGHLLRLRQGLFRCDELFDRIPIDGGPGH
                     GRHYHVADERSTFLIMQDSTLHWTLHAVLDDDAAMAPQFESTIGVPVKYEMLYVGEWK
                     QNLLLADHYGRGRVFLAGDAVHLVIPTGGLGMNSGVGDAIDLGWKLAATLHGWGGPKL
                     LASYEAERRPIGARNVSASRYASLGRRKWRALWRDLDELVRVAEVEQRKSNEMIGAEL
                     SYRYAGSPIIADEPGGPEDDFRRYIPTTWPGARLPHVWLDGHVAIQDRIPEGYALLRL
                     ASRDADVSGLESAMRALGAPFQVLDIADAMAREVYGVDLILLRPDLHVAWRGNQHAAD
                     PVRLARLVTGHSA"
     gene            3851..4807
                     /locus_tag="VFR57_05725"
     CDS             3851..4807
                     /locus_tag="VFR57_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0258900.1"
                     /translation="MASGRQRDSLTDQDKRSKVCRRFHRAVSAGKSSNRVGSNTADLD
                     EGSFMAVSLKAHYSAHDIEARILAALREAGLNPEQRLSPEELAALDHFHTGGLRASRE
                     LQELAQIRGEDRVLDIGAGLAGPARLLAAALGCRVDCIELSPDYCAGAVLLNRLTGLE
                     DRIEVHKGSALDLPFPDDAFDVVWMQNVGMNIADKRRLYREIHRVLKPGGRYAFQEMA
                     AGKAATSYFPLPWATDPSDNFLVSTEEMQSALGESGFIAELFEDTSDVHLGRTTANAT
                     PAAPGQLGLAVFVDNLAQKAANARRSLEEGQVRLVRGVFRVQ"
     gene            4804..5220
                     /locus_tag="VFR57_05730"
     CDS             4804..5220
                     /locus_tag="VFR57_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HEU0258901.1"
                     /translation="MNTMATRRRRKSKSLAAQAIELGVAAPQVIALRAARIAAAGASP
                     SARDREEFYRMGAEKWLALNEAWNSMAAQAFLENQKLALSFVQSLWFPWTHPRSAKAA
                     SKKLTRAGLGILGKGMAPYRRRAVANAKRLGRTKRK"
     gene            5225..5353
                     /locus_tag="VFR57_05735"
     CDS             5225..5353
                     /locus_tag="VFR57_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258902.1"
                     /translation="MHLRPLCEYDVPAQAFMAIELGPHALRDAAGIVSYHPAFAGL"
     gene            complement(5360..6538)
                     /locus_tag="VFR57_05740"
     CDS             complement(5360..6538)
                     /locus_tag="VFR57_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258903.1"
                     /translation="MSIRYATLLGTALALAPSLVSAQIHAMVNYETKSPESLKVLKTP
                     IAAPARKEGIAIIDVDPKSKTFGQIVQDLELPGDLVAHHIFYNRDQTKAYITALGKPE
                     LRVMDMRKQPLALKTIAVPDCQVGEDVVFSKDNRRWFLTCMGSQAMIVGDAEADKPIQ
                     TVKLSKPYPHGIAIHDGIDRILVTSTVRASDLGDAGETITAIEASTGKVLDSYKVSKK
                     ASPAGEAPVEILFVPGAKTPVAYVTNMYGGSLWTASWSAQKKSFDITLAHDFSAQKAG
                     VPLEIYFSPKSDLMYVTTAKPGHLHIFDLKAGPGKPKLVKSIPAAEGAHHVAFTKDWR
                     YAFVQNALLNLPGMSDGSITIVDLKEQKVIGSVDTLKNKGYNPNSIVLLPKWNHLAGH
                     "
BASE COUNT         1095 a         2175 c         2172 g         1234 t
ORIGIN      
        1 gcctccagga gtcttcgcct tgttccaggg attgcgcgtg atgccggtga gcgggctgtc
       61 ggtcacgccc ttccagccga actcgggcgt ggtggttttg ccgagcagca ccgcgccgtg
      121 ctctcgcagc cgcgccgtgg ctggcgcgtc gtcgttccag gggcccttgg gatcgatcgt
      181 cttcgacccg cgccgcgttg gccagcccct ggtcaggatg aggtccttga tggagacggg
      241 cacgccgtcg agcaggccct tcggctgccc cgcgagccag cgagcttcgg aggcttttgc
      301 ttcattcaag gcattctctg aaaccagact gaatgcattg agaaccggat tgagggcttc
      361 aatgcgcttg agcactgccg cggtgacctc gaccggcgac agcgtcctct tgcggaacgc
      421 atcgacgagc tcgcttgccg tgagcgcgca gaggtcgctc accggcgatc cgcccgcgcc
      481 cttgccagcg ctttgtcgcg ccacagaggc caggcgcgct tgtagtcctg tacgggcgct
      541 gaagctttca cttcggcggc atccaagaaa gtgattcgtt tggataacaa aagcgcttgc
      601 atctgcaagc gcgcgctcat ctcgagatag atgctgcggc cgacggcgcg ctgcaggttc
      661 tcgccgatga cgactgagcc gtggccgcgc atcagcgccg cgggcttctt ccccagggtt
      721 ttcgcgagcg ccgctccaag ctgtggtgtt cttacgagca ggtccgagcc cttcttcgcc
      781 ttgcggatct cgaagttcgg caacccgtcg ccgataaagg aagccatgtg gaagagtgcc
      841 cgcattttca cgccggtcac gctgaatggc accaccgacg gcgagtggtt gtgcaccacc
      901 gcgatcacgt cgggtcgcgc gcggtagatc tcgctgtgga tgaagcgctc gttgaccgag
      961 tcgcgcccgc gcgcgtccag ggcttgcgcg tcgagatcat attccaggat gtccccgcgc
     1021 tgcaccgtct ccggggcgat tgcgcaggcg aggaagaacc gcttagggtc gtcgggcgag
     1081 cggatgctga catggccata cgcgtcgatc acgccgtgct ccgccaggat tcggttcgcc
     1141 gcgaccaggt cgtcgagcag ctcttcgttc atcgctccct cggacgaagt gtggcgcaga
     1201 accgcgctgc cgcggggagc agattcgaaa ttgctcaatc ggccttgatt cctgccgcgc
     1261 tcacaaccct cgcccagcgc tcgagctccc gcttgaggag ctcgctcaag cgctcgggcg
     1321 ggccgccaac cacgctcata ccctgtcggg agaggagctc cgaaagaccg ggatcggcga
     1381 ggagggagtt caggtcggcg ttgatcttcg caacgacgcc ggcgggagtt cccgcgggtg
     1441 cgaacgcgcc gtaccaggtc tcgatttcga gctccggcag accttgctcc ttgagcgtcg
     1501 gtacggcgga gaaggcctgt gagcgcttgg tgctcagcac cgcgagcgcg cgcagttgcc
     1561 cgctcagcac ttgcggctgc gcggtttgca ccgcggcgat cgtggcttgc acatgcccgc
     1621 ccacgacatc gccgacggct cccgcggctg tcttgtaagg cacgtgcacg atgtccatgc
     1681 ccagctcgag cttgaggagc tccatcgcga ggtgctgcgg cccgccgttt ccgggcgacg
     1741 agtagtacag cttgcccggc tcgcgcttcg cgagctcgat gagctcgcgc atcgagcgcg
     1801 cggggagctg cgggttgacg accaccacca ggccgctcgt cgccacgagc gcgaccggtg
     1861 cgaagccctt caccggatcg aacggcactt tgccgagcgc gggcgtggtg ccgaacgagg
     1921 tcgcgacgaa gagcaacgtg tagccgtcgg gcgggctctt cgccacgaac gcggcgccga
     1981 tgttgccggt ggcgccgggt ttgttctccg tcaccacgcc gatcttccag cgctcggcga
     2041 ggcgcgcgcc aagcaggcgg gagaggatgt cggcgccggt tcccggcgtg taggggacga
     2101 tgatgtggac ggcgcgcgag gggaagtcct gcgcgcaagc gactccgaac gcgaaagctg
     2161 cagcgagcgc aacgattccc ctcacgcgca aaattataat cagccgatcg atacggacgt
     2221 cctcatcgtc ggcgcaggtc cggtcgggct cgttctcgcc atcgacctcg gccggagggg
     2281 cgtgcgctgc acgctcatcg agcgcaagcc cgagccgcag ttccttccga agatggagcg
     2341 ttgcaacgcg cggacgatgg agatcttccg ccgcatgggc atcgcgacgc tggtgcgcgc
     2401 cgccggcctg cgcgccgacg tgccgatgga cgtctacatc atttgcgcga tgaacaagcc
     2461 cccgctcctg caccatccgt atccgtcggt cgccgaggcg cggcgcgaga tcgacgcgtg
     2521 caccgacggc tccctgccgc tcgagcccta tcagctcatc tcgcagtaca cgctcgagcc
     2581 gcttctcaca tcggttgcag aaaacctgcc cagcgttacc gtccgctacg gttgtgagtt
     2641 tctctccttc agtcaagacg ccatgggcgt cgttacgcag acatcctcgg acaccattgc
     2701 atccaaatac ctggtcggct gcgacggcgg tgcaagcacc gtgcgtcaac agctcggcat
     2761 cgcgctcagc ggcgaaggcc acctgctgcg tcttcggcag ggcctcttcc gctgcgacga
     2821 gctcttcgac cgcatcccga tcgacggcgg cccggggcac ggccggcact accacgtcgc
     2881 cgacgagcgc tctaccttcc tcatcatgca ggactcgacg ctccactgga cgctgcacgc
     2941 cgtgcttgac gacgacgcgg cgatggcgcc gcagttcgag agcacgatcg gggtgccggt
     3001 gaaatacgag atgctctacg tgggcgagtg gaagcagaac ctgcttctcg ccgaccacta
     3061 cgggcgcggg cgcgtgttcc ttgccggcga tgcggtgcac cttgtgatcc cgaccggcgg
     3121 cctagggatg aatagcggcg tgggcgacgc catcgacctc ggctggaagc tcgccgcgac
     3181 gctgcacggc tggggcgggc cgaagctcct cgcctcgtac gaggccgagc gccggccgat
     3241 cggcgcgcgc aacgtctcgg cttcgcgcta tgcctcgctg gggcggcgca aatggcgcgc
     3301 gctgtggcgc gacctcgacg agctcgtccg cgtggccgag gtcgagcagc gcaaatcgaa
     3361 cgaaatgatc ggcgccgagc tcagctaccg ctacgccggc tcgcccatca tcgccgacga
     3421 gccgggcggg cccgaggacg acttccgtcg ctacatcccg accacctggc ccggcgcgcg
     3481 cctgccgcac gtatggctcg acggccacgt cgcgatccag gaccgcatcc cggagggcta
     3541 tgcgctgtta cggcttgcgt cccgcgatgc ggatgtgtcg gggctagaaa gcgcgatgag
     3601 agcgctgggc gcgccatttc aagtgctcga cattgcggac gccatggccc gcgaggtcta
     3661 cggggtcgac ctcatcctcc tgcgtcccga cttgcacgtc gcctggcgcg gcaaccagca
     3721 tgcggccgac cccgtgcggc ttgcgcggct cgtaaccggc cattcggcat agtggccgag
     3781 aaagacagat ttgcgctgga ctcgacccac gcatgtgcat atccagttag gcttctgtca
     3841 tgaggttcac atggcgtcgg gcagacagcg cgacagtctt acagatcagg acaaacgctc
     3901 taaagtgtgt agacggttcc atcgggctgt ttctgcgggc aaatcaagta atcgagtagg
     3961 ctccaatacg gcagatcttg atgagggatc gttcatggct gtcagtctga aagcgcacta
     4021 ttcggcccac gatatcgagg cgcgaatcct cgctgctctc cgcgaggccg gattgaatcc
     4081 cgagcagcgc ctgtcgcccg aggagctggc ggcgctggat catttccaca ccggcgggct
     4141 gcgagcttcg cgcgagttgc aggagctggc gcagattcgg ggcgaggacc gcgttctcga
     4201 tattggcgcc gggctggccg gtcctgcccg gctgctcgct gctgcgctcg gctgccgcgt
     4261 cgactgcatc gaattgtcgc ccgattactg cgcgggcgcg gtactgctga atcggctaac
     4321 cggccttgag gaccgaatcg aagtgcacaa gggcagcgct ctcgacctgc cctttcccga
     4381 cgatgcgttc gatgtcgtct ggatgcagaa cgtcggcatg aacatcgccg acaagcgtag
     4441 gctctacagg gagatccacc gggtcctcaa gccgggcggc cggtacgcct tccaggagat
     4501 ggctgccggg aaagcggcca cgtcctactt tccgctcccg tgggccaccg acccctccga
     4561 caacttcctt gtctctaccg aagagatgca atcggctctc ggcgagagcg gattcatcgc
     4621 tgaactgttc gaggatacga gtgacgttca cttgggcagg actaccgcaa acgccacgcc
     4681 cgctgcgccg ggacagctgg gcttggcggt gttcgtcgac aatctcgcgc aaaaggccgc
     4741 gaacgcacga cgcagtcttg aggaaggcca ggtccggctt gtcagaggcg tcttccgggt
     4801 gcaatgaaca ccatggcaac ccgccgtaga cgcaagtcga aatcgctcgc cgcacaagcc
     4861 atagagctcg gagtcgctgc gccccaggtc atcgcgcttc gggccgcccg aatcgccgcc
     4921 gccggcgcat cgccgtctgc gcgcgatcgc gaagagtttt accgcatggg cgcggagaag
     4981 tggctcgccc tcaacgaagc ctggaactcc atggcggcgc aggcctttct tgaaaatcag
     5041 aaactcgcgc tttcattcgt tcaatccctg tggttcccct ggacccatcc caggtccgcc
     5101 aaagccgcat caaaaaagct gactcgcgcc gggctgggca tcctcggcaa ggggatggcc
     5161 ccttatcgtc gtcgtgcagt cgcgaatgca aagcgcttgg gtcgcaccaa gcggaagtag
     5221 ggcggtgcat ctccggcccc tttgtgaata cgacgtgccc gcgcaagcgt tcatggccat
     5281 agagctcggg ccacacgctt tacgcgacgc cgcgggcatc gtgtcgtacc acccggcgtt
     5341 cgccgggtta tgacgggcat caatgccctg ccagatggtt ccacttcggc agcagcacga
     5401 tgctgttcgg gttgtacccc ttgttcttca gcgtatcgac gctgccgatg actttctgct
     5461 ccttgaggtc gacgatcgtg atcgagccgt cgctcatgcc tggaaggtta agcagcgcgt
     5521 tttgcacgaa ggcgtagcgc cagtccttgg tgaacgccac gtgatgcgcg ccctcggcgg
     5581 cggggatcga cttgaccagc ttcggcttcc cggggccggc cttgaggtcg aagatatgca
     5641 gatgcccggg cttggcggtc gtgacgtaca tcaggtcgga cttcgggctg aaatagatct
     5701 cgagagggac gccggctttc tgcgccgaga agtcatgcgc cagggtgatg tcgaagctct
     5761 tcttctgcgc gctccaactc gcggtccaaa gagagccgcc gtacatgttc gtgacatagg
     5821 ccaccggcgt cttggcgccc ggaacgaaga gtatctccac cggcgcttcg cccgccggcg
     5881 aggccttctt cgagaccttg tagctgtcca gcaccttgcc ggtgcttgcc tcgatggcgg
     5941 tgatggtttc gcccgcatcg ccgaggtcgc tcgcgcgcac cgtgctggtc accaggatgc
     6001 ggtcgatgcc gtcgtgaatg gcgatgccgt gcgggtaggg cttggagagc ttgaccgtct
     6061 ggatcggctt gtcggcctcg gcgtcgccca cgatcatcgc ctggctgccc atgcaggtaa
     6121 ggaaccagcg gcgattgtcc tttgaaaaca cgacatcctc accgacctgg cagtcgggca
     6181 cggcgatggt cttcagcgcg agcggctgct tgcgcatgtc catcacgcgc agctcaggct
     6241 taccgagcgc ggtgatgtag gctttcgtct ggtcccggtt gtagaagatg tggtgggcga
     6301 cgagatcgcc gggcagctcg agatcctgga cgatctgccc gaaggtcttc gatttcgggt
     6361 cgacgtcgat gatcgcgatg ccttccttgc gcgccggcgc cgcgatcggc gtcttcagga
     6421 ctttcaggga ctcgggggac ttcgtttcat aattgaccat cgcatggatc tgcgccgaca
     6481 cgagcgacgg tgcgagcgcg agcgcggtgc ctaagagcgt tgcatagcgg attgacattg
     6541 atcctccctt ccggcgcgat gatacgcccg tctccgcggt tgcgagcgct cgcgttcgca
     6601 gccgcgctgc tgtttcccgc gctcgcccgg gcgcacggtt tcggcacccg ctacgagttg
     6661 ccgctgcccc tcgcgg
//
LOCUS       DASTPD010000169         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1517628, whole genome shotgun sequence.
ACCESSION   DASTPD010000169 DASTPD010000000
VERSION     DASTPD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517628"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFR57_05745"
     CDS             complement(<1..344)
                     /locus_tag="VFR57_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258904.1"
                     /translation="MKIARILLVALVCGCTSVAKMDTGEQTVGGRLVVQLQGPWNHVS
                     GPGIGPAQTWTMEGLPVDQLLIYAGIKDGEVMHQPDRSGKRKDFSFRATMQPDDVVAM
                     FEGMLTRDGSVF"
     gene            complement(341..1495)
                     /locus_tag="VFR57_05750"
     CDS             complement(341..1495)
                     /locus_tag="VFR57_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0258905.1"
                     /translation="MKRRAFLRAGCACAGALLPPLAGAQEQAIEWDFPPRFTRPDIAS
                     DEGGLWAMMDRAETRLRRSPFAIRDHKLHAYVEDIACRLGASHCPDIRVHIVRTPWFN
                     ASMAPNGMMQVWSGLLLRADNEAQLAAVLGHEIGHYISRHSVERLRDARSRAAFGQFL
                     GLFGVAGLVGQVGVLAGMLAYSRDHEREADRIGAILMQRSGYDVSEAAKVWANLLLET
                     KARPQGTQESIFFATHPGPEEREAALAEFARAHPGGETNQTTWQQMLAPFVREWLGDE
                     VRRGQYEESVALLGRALSRGTMQSEYFHARGEAYRLRAGPGDLEYALADFSAAIAAGN
                     EPPQTHRSVGLVQRARKQMPEAKLSFRRYLELDPQAPDAPMIRTYLEEAL"
     gene            complement(1492..>2142)
                     /locus_tag="VFR57_05755"
     CDS             complement(1492..>2142)
                     /locus_tag="VFR57_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258906.1"
                     /translation="SSALAQSKNLAPGFSALSAGARVAVMPTDIELFSISAGGVPEPK
                     ADWTEAASRHFRAALVDKQKQALKLDVVVLDEKDADDFAEINALHGAIARAISMHHFG
                     PGFLQLPTKDGKLDWSLGESTRAIKKATGADYALFTWVRDSYASAERVATMIALAVLG
                     VGVGGGMQVGYASLVELETGRVLWFNRLQRGSGDLREADKAAETIDALLSNFPRSK"
BASE COUNT          350 a          715 c          691 g          386 t
ORIGIN      
        1 ttgaacaccg agccgtcgcg ggtcaacatg ccttcgaaca tggccaccac gtcgtccggt
       61 tgcatggtgg cgcggaagct gaagtccttg cgtttgcccg agcggtcggg ctggtgcatg
      121 acctcgccgt ccttgatgcc ggcatagatg agaagctgat cgaccggcag tccttccatt
      181 gtccatgtct gcgcgggccc gatgccgggg ccgctcacgt ggttccaggg gccttgcagc
      241 tgcacgacca ggcggccgcc gacggtctgc tcgccggtgt ccatcttggc cacgctggta
      301 cagccgcaaa ctagggcgac cagcagaatt cttgcgattt tcataacgct tcctccaggt
      361 aggtcctgat catcggcgca tcgggtgctt gtggatcgag ctccaggtag cgccggaagc
      421 tgagcttcgc ttccggcatc tgcttgcgcg cgcgttgcac aaggccgacg ctgcgatgcg
      481 tctgcggcgg ctcattgccg gcggcgattg cggcgctgaa atccgcgagc gcatattcga
      541 ggtcgcctgg tcccgcgcgc aggcggtaag cttcgccgcg ggcatggaag tattcacttt
      601 gcatggtgcc gcgcgaaagc gcgcgcccga gcagcgcgac gctctcttcg tattgtccac
      661 gccgcacttc gtcgccgagc cattcgcgca cgaacggtgc gagcatctgc tgccacgtcg
      721 tctggttagt ttccccgccc gggtgggcgc gcgcgaactc ggccagcgcc gcctcgcgct
      781 cctcggggcc cgggtgtgtg gcgaaaaaga tgctttcctg cgtgccttgt ggccgcgctt
      841 tcgtttccag cagaaggttg gcccacacct tggcagcctc ggacacgtcg taaccagagc
      901 gctgcatgag gatcgcgccg atccggtcgg cctcgcgctc atggtcgcgc gagtaggcga
      961 gcatgccggc gagcacgccg acctggccga ccagcccggc tacgccgaac aggccgagga
     1021 actggccgaa agcagccctg gatctcgcat cgcgtagtcg ctcgacactg tgcctggaga
     1081 tgtagtggcc gatctcgtgg ccaagcaccg cggccagctg tgcttcgttg tctgctctca
     1141 gaagcaggcc gctccaaact tgcatcatgc cgttcggcgc catcgaggcg ttgaaccagg
     1201 gcgtgcgcac aatatggacg cggatgtccg ggcaatggct cgcgcccagc cgacacgcga
     1261 tgtcctcgac ataggcgtgc agcttgtggt cccgaatcgc gaacgggctg cgtcgcaaac
     1321 gtgtttcggc gcgatccatc atggcccaca ggccgccttc gtcgctggca atgtcggggc
     1381 gcgtgaagcg cggcggaaag tcccattcaa tcgcctgctc ctgggcgccc gccagcggcg
     1441 gtaaaagcgc tccagcgcag gcgcacccag cacgcagaaa ggcgcgccgc ttcacttcga
     1501 gcgcggaaaa ttcgacagca gggcatcgat cgtctcggcg gccttgtccg cctcgcgcag
     1561 atccccgctg ccgcgctgca ggcggttgaa ccagagcacc cggccggtct cgagctcgac
     1621 caaggatgca tagccaacct gcatgccacc gccgacgccg acgccgagca ctgccagcgc
     1681 aatcatcgtt gccacacgct ccgcgctggc atagctgtcg cgcacccacg tgaacagcgc
     1741 atagtccgcg ccggtcgcct tcttgatcgc gcgtgtcgat tcgcccagcg accagtcgag
     1801 cttgccgtcc ttggtcggca actgaaggaa gccggggccg aagtgatgca tcgagatcgc
     1861 gcgcgcaatt gcgccgtgca gcgcgttgat ctcggcgaag tcgtccgcgt ccttctcgtc
     1921 gaggaccacg acgtctagct tgagcgcctg cttctgcttg tcgaccagcg cggcccggaa
     1981 atggcgcgat gcggcttctg tccagtccgc cttgggctcg ggtacgccgc ccgcgctgat
     2041 ggaaaacagc tcgatgtccg tcggcatgac agcaacgcgc gcgccggccg aaagcgccga
     2101 gaagcccggc gcgaggttct tggattgagc aagagctgaa ct
//
LOCUS       DASTPD010000170         4893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1519043, whole genome shotgun sequence.
ACCESSION   DASTPD010000170 DASTPD010000000
VERSION     DASTPD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519043"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFR57_05760"
     CDS             complement(<1..558)
                     /locus_tag="VFR57_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU0258907.1"
                     /translation="MGAELEPFARLIACEDARIDLARACLLIAQDAYPKLDVERYMDE
                     IERMASRLRERLPQNGGAEERVVALNQFLYDDLGYWGNTEDYYDPRNSYLNEVIERKT
                     GLPITMSILYMELGRRIGLPLEGVSFPGHFLVRLRLRGGMLVLDPFAGGAPQSEDELR
                     GRVKRVIPDGVADDLPAAELPLDQFL"
     gene            complement(558..2096)
                     /gene="murJ"
                     /locus_tag="VFR57_05765"
     CDS             complement(558..2096)
                     /gene="murJ"
                     /locus_tag="VFR57_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0258908.1"
                     /translation="MNLLRILATVSSLTMVSRVLGYVRDFFIARVFGAGLATDAFFVA
                     FKLPNLLRRLFAEGAFSQAFVPLLSEHKHKDEQEARRLIDAVSTLLFLALVVTAALGV
                     ATAPLIVYLTAPGFALDPEKPFALTVSLLRVTFPYIFFISLVALSAGILNTWNRFSVP
                     AITPALLNVSFIVGAAFFAEHFDPPVLVLAWAVFAGGILQLAFQVPFLAKLRLLPRWR
                     LDLSHPGLRRVLLLMLPAAFGVSVSQISLVLNQIFASFLQSGSVSWLYYADRLMELPA
                     GVLGVAVGTILLPSLSKYHAAANASEYSRLLDWGLRVTVLLAVPAAVALAVLALPLIA
                     MLFQYGRFTAEDAWMTRQALVAYSVGLVGIVLVKILAPGFYARQNVVTPVKVGVVTLV
                     VTQCMNLALVGPLRHAGLALAIGLGACLNAALLYRLLRRQGIYAPQPGWLVFLAKVAV
                     SVAVMAAALYFAMGASAWWLSAPWQRKVPAVIGLVLLGTACYAACLLALGFRPRDFSR
                     RAAE"
     gene            2112..2375
                     /gene="rpsT"
                     /locus_tag="VFR57_05770"
     CDS             2112..2375
                     /gene="rpsT"
                     /locus_tag="VFR57_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU0258909.1"
                     /translation="MANIKSARKRAKQALARRDHNMRLRTEVRTAIKSVKKALAAGNK
                     DAAASTLRESQRVIDRIVAKGVLHRNAGDRHKSRLAHALKAMK"
     gene            2460..4457
                     /locus_tag="VFR57_05775"
     CDS             2460..4457
                     /locus_tag="VFR57_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0258910.1"
                     /translation="MPKPASRILVINDEHLVLREFVKGLNAAARTLDNPLGISFVGVT
                     TAKEALQLIESDGNLQAVLVDDTLYSLSTRNSNGKKGAAHPRRLQMSALELVQRITRL
                     RPELDVYIVIAQEKEDDVVDALFAESVDGYFYREERDYRGMYRILNAQIQERARTPFY
                     DQLKNYVWMAKDQWHTPGHSSGESLRGSPWVNDFYEFMGEHVFDADLSVSVPMLDSLM
                     EPKGVIGEAQAMAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLILDRNCHKSVH
                     HGVVLSGAHPVYLDSALNKKYGLYGPVPKKALLDAIRKHSDAQALILTSCTYDGLRYD
                     LAPIIEAAHSRGMKVIIDEAWYGFARFHPYFRPTALEAGADYVTQSTHKVLSAFSQAS
                     MIHVNDPDFREHLFRENFNMHTSTSPQYGLIASLDIARKQAVMEGYKLLARTLDLAHE
                     VKKLINSTGVFRVLELKDLLPEAVRHDGIRLDPTKVTIDISGCGYTVEDLQKALFERF
                     NIQVEKSTFNTLTLLLTIGTTRSKVSRLYDALMRIAREGHAPRRLVQTPDIPAFTRLR
                     FLPRDAYYCGGELVQVFDEHERVNRRLAGRVAADQIVPYPPGIPLLVPGQLITREIVD
                     YLGALLHSHKRMEMHGVVHEGYVPCIRVLRSMEEASLQRLL"
     gene            4565..>4893
                     /locus_tag="VFR57_05780"
     CDS             4565..>4893
                     /locus_tag="VFR57_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0258911.1"
                     /translation="MSHVMNTYARQPVAFVRGEGVWLWDETGKKYLDALAGIAVNTLG
                     YGHPKLTKALTQRIDSGVLHTSNLWRIPDQERAADRIAEITGLDEVFFCNSGLEANEA
                     AIKVARK"
BASE COUNT         1002 a         1599 c         1557 g          735 t
ORIGIN      
        1 gaggaactgg tcgagcggca gctccgcggc cggcaggtcg tcggccacgc cgtccgggat
       61 cacgcgcttg acgcggccgc gcaactcgtc ctcggactgg ggtgcgccgc cggcaaacgg
      121 atcgagcacc agcatcccgc cgcgcagtcg caggcgcacc agaaagtgcc cgggaaatga
      181 caccccttcg agcggcagac cgatgcgccg gccgagctcc atgtaaagga tcgacatcgt
      241 gatcgggagg ccggtcttgc gttcgatgac ctcgttcagg tagctgttgc gcggatcgta
      301 atagtcctcg gtattccccc agtagccgag gtcgtcgtaa aggaactggt tcagcgccac
      361 gacgcgctcc tcggcgccgc cgttctgcgg caggcgctcg cgcagccggc tcgccatacg
      421 ttcgatctcg tccatgtagc gctcgacgtc gagcttcggg taggcgtcct gcgcgatcag
      481 caggcacgcg cgcgcgaggt cgatgcgcgc atcctcgcat gcgatcaggc gggcaaaagg
      541 ctctagctca gcacccatca ctcggccgca cggcgcgaga agtcgcgcgg ccggaagccc
      601 aaggcgagca ggcaggcggc atagcacgcc gttcccagca acacgaggcc gatcacggca
      661 ggaaccttac gctgccacgg cgcggatagc caccatgccg atgcacccat ggcgaagtag
      721 agcgctgccg ccatgactgc aacggatacg gcaaccttcg cgaggaacac gagccaacct
      781 ggctggggcg cataaatccc ttggcgccgc agaaggcgat acaagagcgc ggcgttgaga
      841 caagcaccca ggccgatcgc aagcgcgagg cccgcatgcc gcaacgggcc gacgagggcc
      901 agattcatgc attgcgtgac gaccaaggtc acgacgccga ccttcacggg cgtcacgacg
      961 ttctgccgtg catagaaccc cggcgccaga atcttgacca gaacgatgcc caccaggccg
     1021 acgctatagg caacgagcgc ctggcgggtc atccaggcgt cttcggccgt gaagcggccg
     1081 tactggaaca gcattgcgat cagcggcagc gcgagaaccg caagagccac cgccgccggc
     1141 acggcgagca gcacggtaac gcgcaggccc cagtcgagca gccggctgta ttcggacgca
     1201 ttcgcggctg cgtggtactt ggaaaggctc ggcaacagga tcgtgccgac cgcgaccccg
     1261 agcacgcccg ccggcagctc catcagtcgg tccgcgtagt acagccacga tacgctcccg
     1321 ctctgcagga atgaggcgaa gatctggttg agaacgagcg agatctggct gaccgacacg
     1381 ccgaacgccg cgggcaacat gagcagcagc acccgccgca ggcctggatg cgaaagatcg
     1441 agccgccagc gcggcaggag ccgcagcttg gcgaggaagg gcacctgaaa cgcgagctgc
     1501 aggatgccgc cggcgaacac cgcccaggcg agcaccagca ccggaggatc gaaatgctcc
     1561 gcgaagaaag ctgcgcccac gatgaaggag acgttaagga gcgccggcgt aatggccggc
     1621 accgagaagc ggttccacgt attgagaatg cctgcggaga gcgctaccag cgagatgaaa
     1681 aatatgtagg gaaacgtgac ccgcagcagc gatacggtga gcgcaaacgg tttttccggg
     1741 tcgagggcga accccggagc ggtgaggtac acgatcaggg gcgcggtcgc gacgccgagc
     1801 gcggcggtca cgactaacgc gaggaacagc agcgtcgaaa ccgcgtcgat caggcgccgc
     1861 gcctcctgct catccttgtg cttgtgttcg ctcagaagcg gcacgaacgc ctgcgagaag
     1921 gcgccctcgg cgaaaagccg ccgcagcagg ttcggaagct tgaaagcgac gaagaacgcg
     1981 tccgtcgcca gcccggcgcc gaaaacgcgg gcgatgaaga aatcgcgcac gtagcccagt
     2041 acccggctca ccatcgtcaa actgctcacg gtggcgagaa tgcgcagcag gttcatgcta
     2101 gaattttttc aatggcaaac atcaaatccg cccggaagcg cgcgaaacaa gcgctcgcgc
     2161 gccgtgacca caacatgagg ctgcgcaccg aggtgcgtac cgcgatcaag agcgtcaaga
     2221 aggcgcttgc cgccggcaac aaggatgcag ccgcaagtac cttgcgcgag tcgcagcgcg
     2281 tgattgaccg catcgtggcg aaaggcgtcc tgcaccgcaa cgccggcgat cgtcacaaga
     2341 gccgcctggc gcacgcgctg aaagcgatga agtagtcgat agttaagaag ccgtatcccc
     2401 gactgactgc gtttccggcg aaggcctcgg gcatgcgcga ttataagtaa cgtcgcctga
     2461 tgcccaagcc cgcgagccgc attctcgtca tcaacgacga gcatctcgtg ctgcgcgagt
     2521 tcgtcaaggg gttgaacgcc gcagcgcgca ccctcgataa tcccctgggc atcagcttcg
     2581 tcggcgtcac caccgccaag gaagcgcttc aactcatcga gtccgacggc aacctgcagg
     2641 ccgtcctcgt ggacgacacg ctctattccc tgtcgacgag gaacagtaac ggaaagaagg
     2701 gcgcggcgca cccgcgcagg ctgcaaatgt ccgcgctcga gctcgtgcag cgcatcacgc
     2761 gcttgcggcc cgagctcgac gtctacatcg tcatcgcgca agagaaggag gacgacgtag
     2821 tggacgcgct ctttgccgag tcggtcgacg ggtattttta ccgcgaggag cgcgactacc
     2881 ggggcatgta ccgcatcctc aacgcccaaa tacaggagcg tgctcgcacg ccgttctacg
     2941 accagctcaa gaattacgtc tggatggcga aggaccagtg gcatacgccg gggcattcgt
     3001 cgggcgagtc tctgcggggc agcccgtggg tgaacgattt ctacgaattc atgggcgagc
     3061 acgtgttcga cgccgacctt tcggtatcgg tgccgatgct cgattcgctg atggagccga
     3121 aaggcgtgat cggcgaggcg caggcgatgg cggccaaagc cttcggtgcg cgacgcacct
     3181 ttttcgcgac caacggcacc tccaccgcca acaaggtgat cttccagacg ttgctcgcgc
     3241 cgggcgaaaa gctgatcctc gaccgcaact gccacaagag tgtgcaccac ggcgtcgtac
     3301 tgtcgggcgc gcacccggtg tacctcgact cggcgctcaa caagaaatac gggttgtacg
     3361 gcccggttcc caaaaaggcg ctgctcgatg ccatccgcaa gcactcggac gctcaggcgc
     3421 tcatcctcac ctcgtgcacc tacgacggcc tgcgctatga cctggcgccg atcatcgagg
     3481 cggcgcacag ccgcgggatg aaggtaatca tcgacgaggc ctggtatggc ttcgcgcgct
     3541 tccacccgta cttccggccg accgcgctcg aggcgggcgc cgactacgtc acgcagtcga
     3601 cgcacaaagt gctttccgcg ttctcgcaag cctcgatgat ccacgtgaac gacccggact
     3661 tccgcgagca tctgttccgc gagaacttca atatgcatac ctcgaccagc ccgcagtacg
     3721 ggctgatcgc gagcctcgac atcgcgcgca agcaagcggt gatggagggc tacaagctgc
     3781 tggcgcgcac gctcgacctc gcgcacgagg tgaagaagct catcaactcc accggcgtgt
     3841 tccgcgtgct cgagctcaag gacctgctgc ccgaggcggt gcgccatgac ggcatcaggc
     3901 tcgacccgac caaggtcacg atcgatatct cgggctgcgg ctatacggtg gaagacctgc
     3961 agaaggcact gttcgagcgc ttcaacatcc aggtcgagaa gtcgaccttc aacaccctca
     4021 ccctgcttct cacaatcggc acgacgcgat ccaaggtctc gcgcctgtac gacgcgctca
     4081 tgcgcatcgc gcgcgagggc cacgcgccgc ggcgtctcgt gcagacgccg gacatcccgg
     4141 ccttcacccg gctgcgattc ctgccgcgcg atgcttacta ctgcggcggc gagctggtgc
     4201 aggttttcga cgagcacgag cgcgtgaacc ggcgcctcgc aggacgtgtc gccgccgacc
     4261 agatcgtccc ctacccgccc ggcatcccgc tgctcgtgcc ggggcaactt atcacgcgcg
     4321 agatcgtgga ctatctcggc gccctgctcc actcgcacaa gcgcatggag atgcacggcg
     4381 tggtgcacga gggctacgtg ccctgcatcc gcgtcctaag gtccatggaa gaggcctcgc
     4441 tgcagcgcct gctctgagag ccggcgcggc agcgctcatg gcgtcgccat atccggcgac
     4501 cggtgctacc atttccccga acggcggctc gggccgccgt tttgcatttg ggagaggacg
     4561 cgcaatgagc catgtgatga acacctacgc caggcagccg gtcgcgttcg tgcgcggcga
     4621 aggcgtgtgg ctctgggacg agacggggaa gaaatacctc gacgccctgg ccggcatcgc
     4681 ggtcaatacg ctcggctacg ggcacccgaa gctgacgaag gcgctgaccc aaaggatcga
     4741 ctccggggtt ctgcacactt ccaacctctg gcgcatcccg gaccaggaaa gggccgccga
     4801 tcgcattgcc gagatcacgg ggctcgacga ggtgtttttc tgcaactccg gactggaggc
     4861 gaacgaagcg gcgatcaagg tggcgcgcaa gta
//
LOCUS       DASTPD010000171         5930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_703038, whole genome shotgun sequence.
ACCESSION   DASTPD010000171 DASTPD010000000
VERSION     DASTPD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703038"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /gene="alaS"
                     /locus_tag="VFR57_05785"
     CDS             <1..1927
                     /gene="alaS"
                     /locus_tag="VFR57_05785"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0258912.1"
                     /translation="PSVDTGMGLERIAAVLQGKHSNYDIDLFQDLIKAAAHETRQREL
                     KSPSLNVIADHIRACSFLIVDGVIPGNEGRGYVLRRIIRRAIRHGYQLGQKQPFFFRL
                     VDDLDRVMGDAYPELRSAKSRVSAVLRQEEERFAETLDNGMAVLESALASGEKLLDGD
                     TVFRLYDTFGFPVDLTADIARERGVRIDMAGFEAAMDQQRERARAASKFAAASALEYE
                     GSKTEFHGYDTLTLPAKVVALYREGTKVQALKSGEHSTVVLDRTPFYAEAGGQVGDKG
                     ELVSSGGTFEVADTQKIQADVYGHQGTLKTGTLKVGDYVEARVDAALRARTMRNHSVT
                     HLMHKALREVLGPHVQQKGSLVDPDKTRFDLSHDKPVTETELREVEHRVNAEILQNTP
                     TRAQVMPIEEAKNSGAMMLFGEKYGDEVRVLDIGSSREFCGGTHVARTGDIGLFKIYS
                     EGGVAAGIRRIEATTGSTALAIVERQLRELAQVAKQLRAQPGVVEKAVGALLEEKKAL
                     EKELGRLRSRLALGQGQDLAAQAVDVKGAKVLVATLDGADAKTLRETMDKLKDRLKSA
                     AIVLGAVNDGKVALIAGVTADLTGKLKAGELVNYVAQQVGGKGGGRPDMAQAGGTEPA
                     KLPVALQSVKSWVEQRV"
     gene            1924..2265
                     /locus_tag="VFR57_05790"
     CDS             1924..2265
                     /locus_tag="VFR57_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEU0258913.1"
                     /translation="MKRVLFAGVLAFPVAAFAHHGWSEYDSSHELKLTGKIVEAGYEH
                     PHGHVSLDAAGKIWQVVLAPPSRMERRGLEKGALKPGATATVVGYPNRNKPEEMRAER
                     IIIDGKTVELR"
     gene            2262..2762
                     /locus_tag="VFR57_05795"
     CDS             2262..2762
                     /locus_tag="VFR57_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEU0258914.1"
                     /translation="MTPSGGALGALEASGLGQAMRQWLWLYPSVEVVHIVGIGLLFGS
                     IAVLDLRLLGFSRGISVRTLARHVLPWTAGAFVLIVPSGLAMFTAHATEFVQSEVFVV
                     KMLLVLAAGINAALFHTITFRTAGVWDSEEMRKLPPPPSARAAGAISLFLWISVIACG
                     RLLAYF"
     gene            complement(2759..3724)
                     /locus_tag="VFR57_05800"
     CDS             complement(2759..3724)
                     /locus_tag="VFR57_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0258915.1"
                     /translation="MPANVAIPIAGFKDVYDVSSVEKALQELPPSANEALRSLYEKMV
                     RLGGQRFTVKPSALPEMERLFAELPNFAEVLADIRKHLALCIDSNDSIELPPILLLGE
                     PGIGKTHFARKVAELLGTGFGFVPMSSLTAGWVLSGASSQWKNAKPGKVFDTFVNGDY
                     ANPVIVVDELDKASGDGQYDPLGALYELLEVKTATRFVDEFAELPIDASGAVWLATAN
                     DPGRIPEPLLNRLTVYEIDAPDAAGSRTIATAIYREIRNSHDWGRQFPEVPSESALDK
                     LASMPPREMRRTLQGAFGTAKLAGRSELGPDDIQDPRSKKQRIGF"
     gene            3827..4252
                     /locus_tag="VFR57_05805"
     CDS             3827..4252
                     /locus_tag="VFR57_05805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0258916.1"
                     /translation="MATLTREHFNSFGQRHLPGLMGVEIVTVSGECVESRMPVRREVM
                     APNGFLHAASVVALADTSCGYGCVATLPDGAKGFTTIELKANFLGTAREGAIACRATP
                     VHLGRTTQVWDAVVSNEATGAKIALFRCTQMVLWQNPKS"
     gene            4390..5475
                     /locus_tag="VFR57_05810"
     CDS             4390..5475
                     /locus_tag="VFR57_05810"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0258917.1"
                     /translation="MNEPINDKTSLTDDERIHNIVPLPPPEHLIRFFPIQGTAVESQI
                     AETRANVRQILHGRSDRLLVVIGPCSIHDPAAATAYAERLAGERKRHARELEVLMRVY
                     FEKPRTTVGWKGLINDPYLNGSFRINEGLRIARDLLVRINQHGVPAGCEFLDVISPQY
                     IGDLVSWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNVKIAVDALLAARQKHHFL
                     SVHKNGQVAIVETRGNDDCHIILRGGKNPNYDTASVQAACAELARAKLPERLMIDCSH
                     ANAAKDYRRQAAVASDIGRQVAGGERRIIGAMVESHLVEGRQDLAPGKPLVFGQSITD
                     SCLGWDESVRLLEHLAEAVKERRKQSR"
     gene            5501..>5930
                     /locus_tag="VFR57_05815"
     CDS             5501..>5930
                     /locus_tag="VFR57_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0258918.1"
                     /translation="MTERPQQLMHLEYLGDATQFAGQMHALIPKCALLENFSAAEVRL
                     LAHFMGVYRAEAATEIIREGDGGDFMLMVLEGRVEVYKRDRWSTPQLLASVDAGRTLG
                     EMSMIDGEPRFATCLAAEPTLIAVLDRESLARIIVEQPLLG"
BASE COUNT         1063 a         1896 c         1967 g         1004 t
ORIGIN      
        1 gccctcggtc gatactggca tgggcctgga gcgcatagcc gcggtgctgc agggcaagca
       61 ctcgaactac gacatcgatc tcttccagga ccttatcaag gcggccgcgc acgagaccag
      121 gcagcgcgag ctgaaaagtc cgtctctcaa tgtcatcgcc gaccatattc gcgcgtgcag
      181 tttccttatc gtcgacggcg tgattcccgg caacgaaggc cgcggttatg tcctgcgcag
      241 aatcatccgc agagcgatcc ggcatggcta ccagctcggc cagaagcagc ccttcttctt
      301 ccgcctggtc gacgatctgg accgggtcat gggcgatgcg taccctgaac ttcgctctgc
      361 aaagagcaga gtttctgccg tgctgaggca ggaagaagag cgcttcgccg agacgctgga
      421 caacggcatg gcggttcttg agtccgcgct cgcctccggc gagaagctgc tcgacggcga
      481 cacggtgttc cggctctatg acacattcgg cttcccggtc gacctgacag cggacatcgc
      541 ccgggagcgc ggcgtgcgca tcgacatggc cgggttcgag gccgcgatgg accagcagcg
      601 cgagcgcgcg cgcgcggcgt cgaagttcgc cgccgcctcg gcgctcgagt acgagggaag
      661 caagaccgag ttccacggtt acgacacgct gacccttccc gccaaggtcg tcgcgctcta
      721 ccgcgaaggg accaaggtcc aggcgctgaa aagcggcgag cacagcaccg tagtgctcga
      781 ccgcacgcct ttttatgccg aggccggcgg ccaggtcgga gacaagggcg agctggtcag
      841 ttccggcggc acgttcgagg tcgccgatac gcagaagatc caggctgacg tctacggcca
      901 ccagggcacg ctcaagaccg gcaccctcaa ggtcggcgac tacgtggagg cgcgcgtcga
      961 cgccgcgctg cgcgcgcgca ctatgcgcaa ccactcggtc acccatctca tgcacaaggc
     1021 gctgcgcgag gtgctcggcc cgcacgtgca gcagaagggc tcgctggtcg acccggacaa
     1081 gacgcgcttc gatttgtcgc acgacaagcc ggtcaccgaa accgagctgc gcgaggtcga
     1141 gcaccgggtc aacgccgaga tcctgcagaa cacgccgacg cgcgctcaag tgatgccgat
     1201 cgaagaggcg aagaactccg gcgcgatgat gctcttcggc gagaaatacg gtgacgaagt
     1261 gcgtgtgctc gacatcggct cgtcgcgcga gttctgcggg ggcacgcacg tcgcgcgcac
     1321 cggggacatc gggctcttca agatctactc cgaaggtggc gtcgccgccg ggatccggcg
     1381 catcgaagca acgaccggct cgaccgcgct cgccattgtc gagcggcagc tgcgcgagct
     1441 cgcgcaggtg gcgaagcagt tgcgggccca gcccggggta gtggagaagg ccgtgggcgc
     1501 gctgctggag gaaaagaagg cgcttgagaa ggagctcgga cgactgcgct cgaggcttgc
     1561 cctcgggcag ggccaggacc tcgccgcgca ggcggtggat gtgaagggcg ccaaagtcct
     1621 cgtcgccacg ctggatggcg cggacgcgaa gaccctgcgc gagaccatgg acaagctgaa
     1681 ggacaggctg aaatcggcgg cgatcgtgct cggcgcggtg aacgacggca aggttgcgct
     1741 cattgctggc gtaaccgcgg acctcaccgg gaaactcaag gcgggcgagc tggtcaacta
     1801 tgtggcgcag caagtcggcg gcaagggcgg gggccggcct gacatggcgc aggcgggcgg
     1861 taccgagccc gccaagttgc cggtcgcgct gcaatcggtc aagtcatggg tggagcaacg
     1921 cgtatgaaga gagtgctttt tgccggagtc cttgcgtttc cggtggcggc tttcgcacac
     1981 cacggctgga gcgagtacga ctcctcgcac gagctcaagc tcaccggcaa gatcgtcgag
     2041 gccggctacg agcatcccca cgggcacgtc agcctcgatg ccgcgggcaa gatctggcag
     2101 gtggtgcttg ccccgccttc acgcatggag cgccgcggcc tcgaaaaagg cgcgctgaag
     2161 cccggcgcga cggccaccgt ggtcggctat cccaatcgaa acaagccgga agagatgcgc
     2221 gccgaacgca tcatcatcga cgggaagacc gtagagctta gatgacgccg tcgggcggtg
     2281 cgctcggcgc gctggaggcc tccggccttg gccaggccat gcggcagtgg ctgtggctct
     2341 acccgagcgt tgaggtcgtg cacatcgtcg gcatcgggct gctgttcggc tcgatcgcgg
     2401 tgctcgacct gcggttgctg ggcttctctc gcggcatttc cgtaagaacg ctcgcgcgcc
     2461 atgtccttcc ctggaccgcg ggagccttcg tcctcatcgt cccgagcggg ctggcgatgt
     2521 ttaccgccca tgccaccgaa ttcgtccaga gcgaggtgtt cgtcgtcaag atgctgcttg
     2581 tcctggcagc cggcataaac gccgcgcttt ttcacacgat cacgttccgt accgcaggtg
     2641 tctgggattc cgaggaaatg cgcaagctcc cgcctccgcc gtcggcgcgc gccgcgggcg
     2701 cgatctcgct tttcctgtgg atttcggtaa tcgcctgcgg gcgattactg gcgtatttct
     2761 agaaacctat tcgctgcttc ttcgaacgcg ggtcctgaat gtcgtccggc ccgagctcgg
     2821 agcggccggc gagcttggcc gtgccgaacg cgccctgcaa cgtccggcgc atctcacgcg
     2881 gtggcatcga ggcgagcttg tccagcgcgg actccgaggg cacctcgggg aactgccgcc
     2941 cccagtcgtg ggaattgcgg atctcgcggt agatggctgt cgcgatggtg cgcgagccgg
     3001 ctgcgtcggg cgcgtcgatc tcgtacaccg tgagccgatt gagcagcggc tcggggatgc
     3061 ggcctggatc gttcgccgtg gcgagccaca ctgcgcccga ggcgtcgatc ggcagctcgg
     3121 cgaactcgtc gacgaagcgc gtcgcggtct tcacttcgag cagctcgtag agcgcgccca
     3181 gcggatcgta ctgcccgtcg ccgctcgcct tgtccagctc atcgaccacg atcaccgggt
     3241 tggcgtagtc gccgttcacg aaggtgtcga acaccttgcc cggcttcgcg ttcttccact
     3301 gtgaggaagc gcccgaaagc acccagccgg cggtcagcga gctcatcggc acgaagccga
     3361 agcccgtgcc gagcagctcc gcgaccttgc gcgcgaagtg ggtcttgccg atgccgggct
     3421 cgccgaggag cagtatcggc ggcagctcga tggagtcatt cgaatcgatg cacagcgcga
     3481 gatgcttgcg gatgtcggcg agcacctcgg cgaagttcgg cagctcggca aacaggcgtt
     3541 ccatctcggg cagcgccgag ggcttgaccg tgaagcgttg cccgcccagg cgcaccatct
     3601 tctcgtacag cgagcgcagc gcctcgttcg cgctgggcgg cagctcctgc agcgcctttt
     3661 cgaccgacga gacgtcgtag acgtccttga agcctgcgat gggaatggcg acattagccg
     3721 gcatatttat ttggaaaagc tagcacgatg ctctcgtcgt agcaaacacc ttgcctggca
     3781 atgcgttgcg cagagtgcta atcaagtaga gtagttcttc acgactatgg ccaccttaac
     3841 gcgcgagcac ttcaatagtt tcggccagcg gcaccttcct ggtctgatgg gcgtggaaat
     3901 cgtcacggtt tccggcgaat gtgtggagag ccgcatgccg gtgcggcgcg aagtcatggc
     3961 gccgaacggt ttcctgcacg ctgcgtcggt cgtggcactc gccgacacga gctgcggcta
     4021 cggctgcgtc gcgaccttgc ccgacggcgc gaagggcttc acgacgatcg agctgaaggc
     4081 gaacttcctg ggaaccgcgc gcgagggcgc aatcgcctgc cgcgccaccc cggtccacct
     4141 gggccgtacg acccaggtgt gggacgccgt ggtctccaat gaggcgacgg gggccaagat
     4201 tgcgctattc cggtgcaccc agatggtttt gtggcagaat cccaagtcgt gacgcgccgg
     4261 tttttcttta gcttcttctt ctactttccc tgcctgctgg cggaagggac cggctaagct
     4321 ctaactgcga atccaaccaa aaaccgccag cgccaaaagc ctggcggttt ttttttgccc
     4381 gaggccacca tgaatgagcc catcaacgac aagacctccc tcacagacga cgagcgcatc
     4441 cacaacatcg ttccgctgcc gccgcccgag catctgatcc gattcttccc gattcagggc
     4501 accgcggtcg aatcccagat tgccgaaacc cgcgccaacg tgcgccaaat ccttcacggg
     4561 cgctccgacc ggctgctggt ggtgatcggg ccatgctcca tccacgaccc ggcggccgcg
     4621 acggcatacg ccgagaggct cgccggagag aggaaaaggc atgcgcgcga gctggaagtc
     4681 ctgatgcggg tgtattttga gaagccgcgc accaccgtcg gctggaaagg gctgatcaac
     4741 gacccgtacc tgaacggcag ctttcgcatc aacgaaggct tgcgaatagc gcgcgatctt
     4801 ctcgtgcgca tcaaccagca tggcgtgccg gcgggatgcg agttcctcga cgtcatttct
     4861 ccgcagtaca tcggcgatct tgtgtcctgg ggcgctatcg gcgcccgcac caccgagagc
     4921 caggtgcatc gggagctcgc ttcagggctg tcggccccgg tggggttcaa gaacggcacc
     4981 gacggcaacg tcaagatcgc ggtggatgcg ctgctcgctg cgcgccagaa gcaccacttc
     5041 ctctcggtgc acaagaacgg ccaggtggcg atcgtggaga cgcgcggcaa cgacgactgc
     5101 cacatcatcc tgcgcggcgg aaagaacccg aattacgaca cggcgtccgt gcaggcggcg
     5161 tgcgcggagc tcgcgcgcgc gaagctgccc gagcgcctga tgatcgactg ctcgcacgcc
     5221 aacgcggcca aggattaccg ccgtcaggcg gcggttgcct cggacatcgg ccggcaggtc
     5281 gcgggcggcg agcggcgcat tatcggcgcg atggtggagt cgcacctggt ggaaggccgc
     5341 caggacctcg cgcctggcaa gccgctcgtc tttggtcaaa gcatcaccga ttcgtgcctg
     5401 ggctgggacg aatcggtaag gctgctcgag cacctggccg aagcggtgaa ggagcgacgt
     5461 aagcaatcga gatagggatt cggcataatc gggcgcgccc atgaccgaac gcccgcagca
     5521 gctcatgcac ctcgagtacc tcggggatgc cacgcagttc gccggccaga tgcatgcgct
     5581 catcccgaag tgcgcgctgc tcgagaactt ctcggctgcg gaggtgcggc tgctcgcgca
     5641 cttcatgggc gtctaccgtg cggaagccgc gaccgaaatc atccgtgaag gcgacggcgg
     5701 cgacttcatg ctcatggtgc tcgagggccg tgtcgaggtg tacaaacgtg accgctggag
     5761 tacaccgcag cttctcgcgt cggtcgacgc gggcaggaca ctgggcgaaa tgtcgatgat
     5821 cgacggcgag ccgcgcttcg ccacctgcct cgctgccgaa cccacgctga tcgcggtgct
     5881 tgaccgcgag agcctcgcgc gcatcatcgt cgagcagccg ctgctcggag
//
LOCUS       DASTPD010000172         5454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1519684, whole genome shotgun sequence.
ACCESSION   DASTPD010000172 DASTPD010000000
VERSION     DASTPD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519684"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1151)
                     /locus_tag="VFR57_05820"
     CDS             complement(3..1151)
                     /locus_tag="VFR57_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0258919.1"
                     /translation="MNLGRENYPELRDAVRELCNRFDSAYWQKIDEQRAYPEEFVDAL
                     TKAGWMSALIPEDYGGSGLSLTEATVIMEEVTRSGGNAGCCHGQMYNMNTLLRHGSPE
                     QKKRYLPKIASGELRLQSMGVTEPGAGTDTTKIKTVAVKKGDRYVVNGQKVWTSRLQH
                     SELMILLARTSAFDAAKKSAGMSIFLVETKSKGLDVRPIRNMVNHETNAVFIDNLEIP
                     GENMIGEEGKGFRYILDGLNAERILIAAECIGDGYWFIDKSCSYAKDRVVFDRPIGQN
                     QGIQFPIAEAYMEIEAANLMRYKASSLFDSHKPCGAEANMAKHLAAKASWEAANVCLQ
                     THGGFGFAAEYDVERKFRENRLYLVAPISTNLILSYLAEHVLDLPRSF"
     gene            complement(1148..1837)
                     /locus_tag="VFR57_05825"
     CDS             complement(1148..1837)
                     /locus_tag="VFR57_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEU0258920.1"
                     /translation="MAIRSDLGWSTADRITVLGKDLPGEILGKVDLGDMAFLELTRRL
                     PNDKESRLFNAMVVTLVEHGITPSALAARLTYLGAPEAMQAAVAAGLSGLGSVFVGSM
                     EGVARMLSENADVDQMKRVPGLGHPLHKPVDPRTVRLFQIARETGFYGKYCERMEEIG
                     RKKNLVVNATGAIGALACELGLDWRAVKGLGVMARAIGLVGHLLEEARQPMAEAVWHQ
                     IEEQASKHLKP"
     gene            complement(1839..2786)
                     /locus_tag="VFR57_05830"
     CDS             complement(1839..2786)
                     /locus_tag="VFR57_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258921.1"
                     /translation="MRTLLLLALFIASSASAQSWPSKPIRYIVPFPPGAFNDTLARTL
                     AADLPKTLGQPVIVENRPGGNTLIATEMAAKSPPDGYTLFGAALPFSVIQSLYKTSFD
                     VTKDFAPITLAGVTPNLLVANPGAPFNDVKGLIAYAKANPGKLNYASTGNGTSNHLSF
                     ELFKAMTGTQVTHVPYKGSAPAVTDLIAGQVQVMFDNTPNVLPHVKAGKLKALGVSSK
                     TRSQFAPDVPSVDEAGVPGYDVTVWFGVLTVAGTPPDIVKRLNTEMVKILTSPEVKER
                     ISKSGVDVVAGTPEHFSGFLKAEVSRWAKVIQEANIKAD"
     gene            complement(2783..3739)
                     /locus_tag="VFR57_05835"
     CDS             complement(2783..3739)
                     /locus_tag="VFR57_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0258922.1"
                     /translation="MKRFFAVLLLLPLTVFAQTYPNKPIRWIVPYTGGGITDVVTRIV
                     TQKMSGPLGQSIVVDNRPGANSILGSDLAAKAAPDGYTMVTVIAGYAANVTLYQGKLP
                     FDPRKDLVPVSLAGIAPLIMTANNNLPAKDVKELVAYAKSNPGKLNFGSSGIGAAAHL
                     TTELLKQTAGIDMVHVPFKGTAPALTALMANDIQILVDVPSSLMPHVRGGKIKALAMF
                     SKERIQGAQEVPTMAEAGGPPLESSTWVLFMAPGGTPKPIIDRLAAETVKAINESDIK
                     ERFSQIGIVPVGNSPPQAAKFLEEEIAKWAKVINTAGVKAEQ"
     gene            complement(3736..4875)
                     /locus_tag="VFR57_05840"
     CDS             complement(3736..4875)
                     /locus_tag="VFR57_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0258923.1"
                     /translation="MKIAAVRAYPTSFPVPPQNRVALGIGTAVKRDAVVVKVTTDEGI
                     IGWGEAHHGRAHTAVAKLIETTLRQLIVGMDALSTTEVWEKLYRAQLASHGMGAGACL
                     AISGIDMALWDIKGKALKQPLFRLLGGAMKAIPAYAGGVSLGYQPEAELVTEARKSLE
                     QGYKAIKLRVGDAPSRDIARMRAVRKAFGDDLVILTDANIGYQLEDVRRVMPVLDEIR
                     AGWLEEPFPAHDYRTYREAKLLGRTPLAAGENHYTRFEFNRVIEDGAITILQPDLSKC
                     GGITEALRIAALASSWKLPIHPHSSMTGLNHAATIHFLAAIGNGGYFEGDVSKSNLFR
                     DELVENPGVVDRDGNVRPLDKPGLGLEVNEAFLAKHPAIEGPGYV"
     gene            complement(4872..>5454)
                     /locus_tag="VFR57_05845"
     CDS             complement(4872..>5454)
                     /locus_tag="VFR57_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0258924.1"
                     /translation="RSLSWDEARRLGAPARELAALTLGIVGVGAIGSRLAKIARNGFG
                     MRVLGHQRRLDRLPSEAEPVSLDNLLAQSDFVVLACPLTPQTRYLINPETIAKMKRTA
                     WLINVGRGPVVQETALIEALREKRIAGAMLDVYEHYRLEPGHPLFQLDNVILTPHLAA
                     VTQESRARASVAAADEMLRILRGERPRNPVTPL"
BASE COUNT          874 a         1691 c         1832 g         1057 t
ORIGIN      
        1 ccttagaagg accgcggcag gtcgagtacg tgctcagcga ggtaggagag gatcaggttc
       61 gtggagatcg gcgccacaag gtacagccgg ttctcgcgga atttcctctc gacgtcgtat
      121 tcggcggcga agccgaaccc gccgtgcgtc tgcaggcaaa cgtttgccgc ttcccatgac
      181 gccttggcgg cgaggtgctt ggccatgttc gcctctgcac cacagggttt gtgggaatcg
      241 aaaagcgaag aagccttgta gcgcatgagg ttcgcggcct cgatctccat gtaggcctcg
      301 gcgatcggga actggatgcc ctggttctgg ccgatggggc ggtcgaagac cacgcggtcc
      361 ttcgcgtagg agcacgactt gtcgatgaac cagtagccgt cgccgatgca ctcggcggcg
      421 atcaggatcc gttcggcgtt aagaccgtcg aggatgtagc gaaagccttt gccctcctcg
      481 ccgatcatgt tctcgccggg gatctccagg ttgtcgatga acaccgcgtt cgtctcgtgg
      541 ttcaccatgt tgcggatcgg gcgcacgtcg aggcccttgc ttttcgtctc cacgaggaag
      601 atcgacatgc cggcggattt cttggcggcg tcgaaggcgc tcgtgcgcgc gagcagaatc
      661 atgagctcgg agtgctgcag gcgcgaggtc cacaccttct ggccattcac cacgtagcgg
      721 tcgcctttct tcaccgctac ggtcttgatc ttcgtcgtgt cggttccggc accgggctcg
      781 gtgacgccca tggattgcag gcgcagttct ccgctcgcga ttttcggcag gtagcgcttc
      841 ttctgttcgg gtgagccatg gcggagcagg gtgttcatgt tgtacatctg gccatggcag
      901 cagccggcgt tgccgccgga gcgcgtcact tcctccatga tcaccgtagc ttcggtgagc
      961 gagagccctg aaccgccgta atcctcgggg atcaacgcgg acatccagcc ggctttggtg
     1021 agagcgtcga cgaactcttc gggataggcg cgctgctcgt cgatcttctg ccagtacgcg
     1081 gaatcgaagc ggttgcagag ctcgcgcacc gcgtcgcgca gctcgggata gttttcgcgg
     1141 ccgaggttca tggtttcagg tgcttcgacg cctgctcttc gatctggtgc cagaccgctt
     1201 cggccatcgg ctggcgcgct tcttccagca ggtgtccgac gaggccgatg gcgcgcgcca
     1261 tcacgccgag gcccttcacc gcgcgccagt cgagccccag ctcgcaggcg agcgcgccga
     1321 tcgccccggt ggcgttcacc acgagattct tcttgcgccc gatctcttcc atgcgctcgc
     1381 agtacttgcc atagaagccg gtttcgcgcg cgatctgaaa aagacggacc gtgcgcgggt
     1441 cgaccggctt gtggagcggg tggccaaggc cgggcacgcg cttcatctga tccacgtcgg
     1501 cgttttcgga aagcatgcgc gcgacgcctt ccatcgagcc cacgaatacg ctgccgaggc
     1561 cgcttaggcc cgcggcgacc gccgcctgca tggcttcggg cgcgccgagg taggtgaggc
     1621 gcgcggcgag cgcgctcggc gtgatgccgt gctcgacgag cgtgaccacc atcgcgttga
     1681 agaggcgcga ctccttgtcg ttcggcaagc ggcgtgtgag ctcaaggaac gccatgtcgc
     1741 cgaggtccac cttgcccaga atttcgcccg gcagatcctt gccgagcacc gtaatgcgat
     1801 cggcggtcga ccagccaagg tcagagcgga tcgccacgct agtccgcctt gatgttcgct
     1861 tcctggatta ccttggccca gcgcgaaacc tcggccttga ggaagccgct gaagtgctcc
     1921 ggcgtgccgg ccaccacatc gacgccggac ttgctgatgc gctccttgac ctccggcgac
     1981 gtgaggatct tcaccatctc ggtgttgagt cgcttgacga tgtccggcgg cgtgccggca
     2041 accgtgagga cgccgaacca cacggtgacg tcgtagccag gaacgccggc ttcgtcgacg
     2101 ctcggcacgt ccggcgcgaa ctgcgagcgc gttttcgacg acacgccgag cgctttcagc
     2161 ttgccggcct tcacgtgcgg cagcacgttc ggcgtgttgt cgaacatgac ctgcacttgc
     2221 ccggcgatga ggtcggtcac cgcaggagca ctgcccttgt acgggacatg ggtcacctgc
     2281 gtcccggtca tcgccttgaa cagctcgaaa gaaaggtggt tggacgtgcc gttgccggtc
     2341 gaggcgtagt tgagcttccc cgggttcgct tttgcgtagg cgatgagtcc ttttacgtca
     2401 ttgaacggcg cacccggatt ggcaacaagc aagttagggg tgacaccggc cagcgtgatg
     2461 ggcgcgaagt ctttcgttac gtcgaacgag gtcttgtaga ggctctggat caccgagaag
     2521 ggcagcgccg cgccgaagag cgtgtagccg tcgggcggcg acttcgccgc catctcggtg
     2581 gcgatcagcg tgttgccgcc cgggcggttc tcgacgatca cgggttggcc cagcgtcttt
     2641 ggcaggtcgg cggccagggt gcgggcgagc gtgtcgttga aagcgcccgg cggaaacgga
     2701 acgatgtagc ggatcggctt gctcggccag gattgcgccg aggcgctact cgcgatgaag
     2761 agcgcgagaa ggagaagcgt tctcattgtt cggcctttac gcccgcggtg ttgatgacct
     2821 tcgcccactt ggcgatctct tcttccagga acttcgccgc ctgtggcggc gagttgccga
     2881 ccggcacgat gccgatctgg ctgaagcgtt ccttgatgtc cgactcgttg atcgctttca
     2941 ccgtttcggc ggcaaggcgg tcgatgatcg gcttcggcgt gccgcccggc gccatgaaca
     3001 gcacccaggt tgaggattcg agcggcggtc cgccggcctc ggccatggtc ggcacttcct
     3061 gggcgccctg gatgcgctcc ttggagaaca tggcgagcgc cttgatcttg ccgccgcgca
     3121 cgtgcggcat gagcgagctc ggcacgtcga cgaggatctg gatgtcgttc gccatcagcg
     3181 ccgtcagcgc gggggctgtg cccttgaagg gcacgtgcac catgtcgatg ccggcggtct
     3241 gcttcaggag ctccgtggtg aggtgcgccg cggcgccgat acccgaggac ccgaaattca
     3301 gctttccggg attcgatttc gcgtaggcga cgagctcctt cacgtccttc gccggcaggt
     3361 tgttgttcgc cgtcatgatg agcggcgcga tgccggcaag cgacacgggc acgagatcct
     3421 tgcgcggatc gaagggcagc tttccctggt agagcgtgac gttggccgcg tagccggcga
     3481 tcacggtcac catggtgtag ccgtcgggcg ccgccttcgc cgcgaggtcc gagccgagga
     3541 tgctgttcgc gccaggacgg ttgtcgacca cgatgctctg gccgagcggt ccggacattt
     3601 tctgcgtgac gatgcgcgtc accacatcgg taatgccacc gcccgtgtag ggcacgatcc
     3661 agcggatcgg cttgttcgga taggtctgcg caaaaaccgt caggggtagg aggagcagga
     3721 cggcgaaaaa tcttttcata cgtaaccggg tccttcgatg gcgggatgct tcgcgagaaa
     3781 ggcctcgttt acttcgagcc cgaggccggg cttgtcgagc ggacgcacgt tgccgtcccg
     3841 gtcgacgact ccgggattct ccaccagttc gtcgcggaaa agattcgatt tcgaaacgtc
     3901 gccttcgaaa tagccgccgt tgccgatggc ggcaaggaag tggatggtgg ccgcgtggtt
     3961 taagccggtc atggagctat gcggatgaat cggcagcttc cagctcgaag ccagcgcggc
     4021 gatgcgcagc gcctcggtga tcccgccgca cttcgagagg tcaggctgca ggatcgtgat
     4081 ggcaccgtcc tcgatcaccc gattgaactc gaaccgcgtg tagtgattct cgccggccgc
     4141 gagcggcgtg cggccgagga gcttcgcttc gcgataggtg cggtagtcgt gcgcggggaa
     4201 gggttcttcg agccagcccg cacggatctc gtccagcacc ggcatgacgc ggcgaacgtc
     4261 ttcgagctgg tagccgatgt tcgcgtcggt aaggatgacg aggtcgtcgc caaacgcctt
     4321 tctcaccgcg cgcatgcgcg cgatgtcgcg cgagggtgcg tctcccacgc ggagcttgat
     4381 tgccttgtac ccctgctcga ggctcttgcg agcttcagtg acgagctcgg cttcaggctg
     4441 gtagccgagc gacaccccgc ccgcgtacgc ggggatcgcc ttcatggcgc caccgagcag
     4501 gcgaaagagc ggctgcttga gcgccttgcc tttgatatcc cacagcgcca tatcgatgcc
     4561 gctgatcgcg aggcaggcgc cggcgcccat gccatggctc gcgagctgcg cgcgatagag
     4621 cttctcccag acctccgtgg tcgaaagcgc gtccattccc acgatgagct gcctgagcgt
     4681 cgtctcgatc agcttggcga ccgcggtgtg cgcgcgcccg tggtgtgcct ccccccagcc
     4741 gataatgcct tcgtcggtgg tcaccttcac gacgaccgcg tcgcgcttga ccgcggtgcc
     4801 aatgccgagc gccacgcggt tttgcggcgg aaccggaaag gaagtcggat atgccctgac
     4861 ggcggcgatc ttcatagggg cgtgaccgga ttgcgcggcc gctcgccgcg caggatgcgc
     4921 aacatctcat cggccgcggc gacgctcgcg cgcgcccgcg actcctgcgt gaccgcggcg
     4981 agatgcgggg tcaggatcac gttatcaagc tggaacagcg gatgcccggg ctccaggcgg
     5041 taatgctcgt agacatcgag catcgcaccg gcgatgcgct tttcgcgcag cgcttcgatg
     5101 agcgccgtct cctgaaccac ggggccgcga ccaacgttga tgagccacgc ggttcgcttc
     5161 atcttcgcga tcgtttccgg gttgataagg tagcgggttt gtggagtgag cgggcaggcg
     5221 aggacgacga agtcgctctg cgcgaggagg ttgtccagcg acacgggttc cgcttcgctg
     5281 ggaagccggt ctaatcggcg ttggtggccg agcacgcgca tcccaaaccc gttgcgcgct
     5341 attttcgcga gccggctgcc gatcgctccc acgccgacga tgccgagcgt aagggcggcg
     5401 agctcgcgcg ccggggcgcc gaggcggcgc gcttcgtccc aggaaagcga acgc
//
LOCUS       DASTPD010000173         7075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_498680, whole genome shotgun sequence.
ACCESSION   DASTPD010000173 DASTPD010000000
VERSION     DASTPD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498680"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1253)
                     /locus_tag="VFR57_05850"
     CDS             complement(153..1253)
                     /locus_tag="VFR57_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0258925.1"
                     /translation="MRVLVAGAGPAGLYFSYLLRRKRPAWEIRVVEQNRADSTFGFGV
                     VFSDRALEFLREDDASTYDLITPQMEAWTDLTVMHRRTPVVIDGIGFAAIGRLKLLGL
                     LQQQLARVGIVPEFEKTLRQDVETEGYDLVVAADGANSFVRGTRAFGTTVEPLSNKFA
                     WFGTTKLFATLTQTFVENEHGAFNAHHYRHSERMSTFVFECDAATWTRAGFERMPEPE
                     TLRYAERLFAETLEGHALVSNRSIWRNFPNIRNERWSSGNTVLIGDALRTAHFSIGSG
                     TRLAMEDAIALVKALDEHPRAEDALAAFEAARRPIVEKLVDAADASGAWYERFPEHMK
                     LTPREFAWSYIQRSGRIDPERLRKTSPRFVDG"
     gene            complement(1301..2374)
                     /locus_tag="VFR57_05855"
     CDS             complement(1301..2374)
                     /locus_tag="VFR57_05855"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0258926.1"
                     /translation="MTTPPARADHVGSLLRPKKLREAFRRHAANEIDDRTFRSIQDEA
                     IRDVVKLQQDCGLQVLTDGEFRRISYWEKFVRLTRGLEVRDAIFTFHDAEGHESKFTA
                     PYVSGEVARKEPITLDELGFGNTKITMPAPSTMHFYRFTDWGSAYDKAENFFRDLGTV
                     YQAEIADLAKAGCRYVQLDEVAVAILCDPAAREKVKAAGEDSDRLVDLYIDAINEAVK
                     NRPAGVTVGVHVCRGNYKGMYLSEGGYDSVAEKFFSRTNVNHFLLEFDSPRAGGFAPL
                     RFVPKSKGVVLGLVSSKTPSLEKMDILKKRAEEAVRYIDSNRLAISPQCGFASTIGGN
                     PLTEADERAKLKLCVDAAREIWR"
     gene            complement(2371..3162)
                     /locus_tag="VFR57_05860"
     CDS             complement(2371..3162)
                     /locus_tag="VFR57_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0258927.1"
                     /translation="MTLPTYQTLALESRDEHVLVVTLNRPQVLNALNTQMGSDKLELW
                     TRLSADPGSWRCVVLTGAGERAFCAGADLKERDGMSEGVWRAQHEIFERAFMALMELP
                     LPVIAAVNGAAFGGGLEMALACDFIYAAKTARMALSETRLGLMPGGAGTQNLPRAAGE
                     RRAKELIFTAEPFDAQQGYDWGIVNRVCDNALQDSLSVASRIAANAPLAVRQAKKSVH
                     YGLQMDLLTGYRFEIEAYNQLVGTEDRKEGVRAFNEKRKPRFQGK"
     gene            3179..3547
                     /locus_tag="VFR57_05865"
     CDS             3179..3547
                     /locus_tag="VFR57_05865"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEU0258928.1"
                     /translation="MRDTSVNKKTALALACASLLSGCAVLSSEAAVVGCQVADTVTTL
                     RAVELGGREANPMVEWLLKQFGPGGFMAAKAAATALFLSVYPDVPSGLVILLNGVTCA
                     VAAHNARIVIRLEEQRGQSE"
     gene            complement(3535..4446)
                     /locus_tag="VFR57_05870"
     CDS             complement(3535..4446)
                     /locus_tag="VFR57_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0258929.1"
                     /translation="MLWITAMNVQDIDLNLLRVFDAVLHEKGVTPAAARLGLTQPAVS
                     NALARLRKLFGDALFVRTPRGVDATPFARELAEPVRQALALLESALAHGPGFDPATSS
                     RAFRFYMSDLGQIEFLPPLVERVQRDAPGVRLEAVALEVDDIADALAAGALDVAIGFL
                     PGLAGAVRRRTLFRDPYLCLMRADHEIKSLTKKSFLNASHALVTYRGGHRVIEEALDR
                     AGLARRIALRVPHFTVVPMVLERTDLILTLPARVARVFERRGKLKALPPPVAIPPAEV
                     AVHWHERFEQDPGNRWLRDLTVELYSD"
     gene            4513..5466
                     /gene="dctP"
                     /locus_tag="VFR57_05875"
     CDS             4513..5466
                     /gene="dctP"
                     /locus_tag="VFR57_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0258930.1"
                     /translation="MKKTLMLVVLAAAPFVAQAQETTLRVVSAFAENTQYVKNLMPMI
                     QALNTEGKGRLQVNFIGGPKAMPPFEVGNAVRTGVVDMAMTTGAFYTNIMPEADALKL
                     TQIPAQELRKNGGYDLINKIWNEKANMQYLGRVIDFTPFHLYLTKKIDKPDLTGLKIR
                     ITPVYRDFFQALGATVMQTAPGEVYTALERGVVDGYGWPINGIFDFNWHEKTKFRVDP
                     GFYNAEVSLVMNLDKWKALNSGQRDLLMKHVIALENANDSWKKVNEDDIRKQKEAGIQ
                     VISFDAATAKQYYAKAYEVAWENAIKASPQYGPQMRKLFSN"
     gene            5470..5997
                     /locus_tag="VFR57_05880"
     CDS             5470..5997
                     /locus_tag="VFR57_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0258931.1"
                     /translation="MGCLASAYGKVLAALALLACAVLFAMMLVICVDVLLRNVRLIPG
                     VYSVAWANEATEYSLYLITLLTAPWLLRQGQHIRVDVFLRIVPPRIAWYCEWLCDLTA
                     FVVCLLIAYASWKATLSSMAINAMVVKTLALPEWWLLAPMQLCFVLLAIEVLFRMARL
                     HAGERGPRDDAVTVA"
     gene            6000..>7075
                     /locus_tag="VFR57_05885"
     CDS             6000..>7075
                     /locus_tag="VFR57_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0258932.1"
                     /translation="MSWQAAAWLMLGGSTALMFLGLPVAFAFLAINLIGAAIYLGGEP
                     GLVQFARNGVQSVTSFALTPIPLFILMGEVLFHTGLAVKVIDGIERLIRHVPGRLAVV
                     AVVAGTVFSAISGSTIATTAMLGSLMLPVMLARGYHPTISTGPIMAIGAVDMLIPPSA
                     LTVLLGSLSGISISKLLVGGILPGLVLSVIFIAYIVVRVRLEPQLAPNTPFEEFHGWR
                     RLQPFVQYVLPLVSIFVIVVGAMIAGWATPTECAALGAFATIVLAVLYRALTLRNLVR
                     ALRGTAGISGMILFIIVGATTFSQILSFSGASSGLAQMITDSGLPPLAIIAGMMLFLI
                     FLGIFVEQVSMMMITLPIFMPIVQ"
BASE COUNT         1222 a         2274 c         2289 g         1290 t
ORIGIN      
        1 atcctgcgct tcttgcagga agggtggttc aacgcgccac agctctcttg cgcgcacgcg
       61 gggcgggaag cggccggcga gctttttgcg gtcgatcagc cactcgggct gagggtagct
      121 gccgacgagc gtggtgagaa aaagcatcaa cgttatccgt ctacgaatcg gggggaggtc
      181 tttctgagcc tctcggggtc gatgcggccg cttctctgaa tatagctcca ggcgaactcg
      241 cgcggcgtga gtttcatgtg ctcggggaag cgctcatacc aggcgccgct cgcgtcggcc
      301 gcgtccacca gcttctccac gatgggccgg cgcgccgcct cgaatgcggc aagcgcgtcc
      361 tccgcccgag gatgctcgtc cagggccttg acgagcgcga tcgcgtcctc catggcgagc
      421 cgcgtgcccg acccgatcga gaaatgcgcg gtgcgcaatg catccccgat gaggaccgtg
      481 ttgccgctgg accatctttc attcctgata ttcgggaagt tgcgccagat ggatctgttg
      541 gagacgagcg catgcccttc gagggtctcg gcgaaaagcc gttccgcgta tctcaaggtt
      601 tccggctccg gcatgcgctc gaacccggcg cgggtccagg tcgcggcatc gcactcgaag
      661 acgaaggtgc tcatgcgctc ggagtgacgg taatgatgag cgttgaacgc gccgtgctcg
      721 ttctcgacga acgtctgcgt gagcgtagcg aacagcttcg tcgtgccgaa ccaggcaaac
      781 ttgttgctga ggggctcgac ggtggtgccg aatgcgcgcg tgccgcgcac gaacgagttc
      841 gcaccgtcgg cggcaaccac gaggtcatag ccttcggttt cgacgtcctg ccgcaacgtt
      901 ttctcgaact cggggacgat gccgacgcgc gcgagctgct gctgcagaag cccgaggagt
      961 ttgagccggc cgatagcggc gaagccaatc ccgtcgatta ccaccggggt gcggcgatgc
     1021 atgacggtca agtcagtcca cgcttccatc tggggggtga tcaggtcgta cgtcgaggcg
     1081 tcgtcctcgc gcaggaattc gagcgcccgg tcggagaaca ccacgccaaa gccgaaggtc
     1141 gaatcggcgc ggttttgctc caccacgcgg atctcccagg cgggccgctt tcgccggaga
     1201 aggtacgaga aatagaggcc cgccggcccg gcgccggcga cgaggacgcg catctactgg
     1261 aaaatcagcc gcagatcgaa gcgtttgccc gcggagaccc tcatcgccag atctcccttg
     1321 cggcgtcgac gcagagcttg agcttggcac gctcatccgc ctcggtgagc gggttaccgc
     1381 cgatcgtgct cgcgaagccg cactgcgggc tgatggcgag cctgttcgaa tcgatgtagc
     1441 ggacggcctc ctcagctcgc tttttcagaa tatccatttt ttctagggac ggcgtcttgg
     1501 aactcaccag gccgagcact acccccttgc tcttcggcac aaagcgaagc ggcgcgaaac
     1561 cgccggcgcg cgggctgtcg aactcgagca gaaaatgatt cacgttcgtg cggctgaaga
     1621 acttttcggc cacggaatcg tagccgcctt cggaaagata catccctttg tagttgccgc
     1681 ggcagacatg cacgccgacg gtaacgccgg ccggacggtt cttcaccgcc tcgttaatcg
     1741 cgtcgatgta gaggtcgacc agccgatcgg aatcctcgcc cgccgccttc actttctccc
     1801 gggccgccgg atcgcacagg atcgcgaccg ccacctcgtc gagctgcacg tagcggcagc
     1861 ccgccttcgc cagatcggcg atctccgcct ggtacaccgt gcccagatcg cgaaagaagt
     1921 tctcggcctt gtcgtacgcg ctgccccagt cggtaaagcg gtagaagtgc atcgtcgagg
     1981 gcgccggcat cgttattttc gtattgccaa agccaagctc gtcgagggtg atcggttctt
     2041 tccgcgccac ctcgccgctc acatagggag cggtgaactt cgactcatgg ccctcggcgt
     2101 cgtggaaggt gaagatggcg tcgcgcacct cgagcccgcg cgtgagccgc acgaacttct
     2161 cccagtacga gatgcggcga aactcgccgt cggtcagaac ttggaggcca caatcctgct
     2221 gcagtttgac gacatcccga atcgcctcgt cctggatcga gcgaaatgtc ctgtcatcga
     2281 tttcattcgc agcatgccgg cggaacgcct cccggagctt ctttggccgc agcaggctgc
     2341 cgacgtggtc tgcgcgggcc ggcggcgtcg tcacttgccc tgaaatcgcg gcttgcgctt
     2401 ctcgttgaag gcgcgcacgc cctcttttcg gtcttcggtg ccgacgagct ggttgtaggc
     2461 ttcgatctcg aagcggtagc cggtgaggag atccatttgc aggccgtagt gcaccgactt
     2521 cttcgcctgg cgcactgcga gcggggcatt agcggcgatt cttgacgcaa cactcaaaga
     2581 atcctgcaac gcgttgtcgc aaacccggtt cacgatgccc cagtcatagc cctgctgcgc
     2641 gtcgaacggc tcggcggtaa aaatgagctc cttcgcccgg cgttccccgg cggcgcgcgg
     2701 caagttctgc gtgccagcgc cgcccggcat gaggccgagg cgcgtctccg agagcgccat
     2761 gcgcgcggtc ttcgcggcgt agatgaagtc gcaggcgagc gccatctcga gcccgccgcc
     2821 aaacgcagcg ccgttcaccg cagcaatcac tggcaggggc agctccatga gcgccatgaa
     2881 ggcgcgctcg aagatttcgt gctgcgcgcg ccacacccct tcgctcatgc cgtcgcgttc
     2941 cttcaagtcc gcgccggcgc aaaaggcgcg ctcccccgcg ccggtcagca ccacgcagcg
     3001 ccagcttccc ggatcggcgc tgaggcgggt ccagagctca agcttgtcac tccccatctg
     3061 cgtattgagc gcgttcagca cctgcgggcg gttgagcgtg acgacgagca cgtgctcgtc
     3121 gcggctctcg agagcgagcg tctggtaggt gggaagcgtc atcaattaca tttagctcgt
     3181 gcgcgacacc agcgtcaaca agaagacggc tctggcgctg gcgtgcgcgt ccctgctaag
     3241 cggctgcgcc gttctctcca gcgaggcggc cgtcgtcggc tgccaggtgg cagataccgt
     3301 cacgacgttg cgcgcggtcg agctcggcgg gcgcgaggcg aatccgatgg tcgaatggct
     3361 gctcaagcaa ttcggcccgg ggggattcat ggcggccaag gccgccgcga cggcgctctt
     3421 cctgagcgtc tatccggacg tgccttcggg gctcgtcatc ctgctcaacg gcgtgacctg
     3481 cgcggtggcg gcccacaacg cgcgcatcgt tatccggctt gaagaacaaa gaggtcagtc
     3541 ggagtagagc tcgaccgtca ggtcgcgcag ccaccggttg ccggggtctt gctcaaagcg
     3601 ctcgtgccag tgcaccgcga cttccgcagg gggaatggcg accggcggcg gcagagcctt
     3661 gagtttgccg cgccgctcga acacgcgcgc gacgcgtgcc ggcagcgtga gaatcaggtc
     3721 ggtgcgttca agcaccatcg ggaccacggt gaagtgtggc acccggagag cgatccggcg
     3781 cgcgaggccg gcacggtcga gcgcctcctc aatcacccgg tggccgccgc gatacgtgac
     3841 cagcgcgtgg gatgcattaa ggaaactttt cttggtcagt gacttgattt cgtggtccgc
     3901 cctcatcaag cacaggtagg gatcccgaaa cagcgtgcgg cggcgcacgg cgccggcgag
     3961 gccgggcaga aacccgatcg cgacatcgag tgcgcccgcg gctagcgcgt cggcgatgtc
     4021 gtcgacctcg agcgcgaccg cctccaggcg cacgccgggc gcatcgcgct gcacgcgctc
     4081 gacgagcggc ggcagaaact cgatctgacc caggtcggac atgtagaacc ggaacgcacg
     4141 gctcgaggtc gccgggtcga agcccgggcc gtgcgcgagc gcagactcga ggagcgcaag
     4201 cgcctgcctc accggctcgg caagctcgcg cgcgaacgga gtggcgtcca cgccgcgagg
     4261 cgtgcgcacg aaaagcgcgt cgccgaagag cttgcgtagc cgtgcgagcg cgttggacac
     4321 cgccggctgc gtgagcccga gacgcgccgc cgccggcgtc acgcccttct catggagcac
     4381 cgcatcaaac acgcgcagca ggttgaggtc gatgtcctgc acattcatgg ctgtgatcca
     4441 taacatgaat gacattgatt tgacaaatgt aagcgcgcaa ccctacgctc acccttcttc
     4501 ctaggaggag tcatgaagaa aacgctgatg ctggtggtcc tggcggcggc gccgttcgtg
     4561 gcgcaggcac aggagacgac gctgagggtg gtaagcgcgt tcgccgagaa cacgcagtac
     4621 gtgaaaaacc tgatgccgat gattcaggcg ctgaacacgg agggcaaagg gcgcctccaa
     4681 gtcaatttta tcggcgggcc aaaagcgatg ccgccgttcg aggtcggcaa cgcagtgcgc
     4741 accggcgtgg tcgacatggc gatgaccacc ggggcgttct acaccaacat catgccggag
     4801 gccgatgccc tcaagctcac gcagatcccg gcgcaggagc tgcgcaagaa cggcggctac
     4861 gacctcatca acaagatctg gaacgagaag gccaacatgc agtacctcgg ccgggtcatc
     4921 gacttcacgc cgttccatct ctacctcacg aagaagatcg acaagcccga tctcaccggc
     4981 ctcaagatcc gcatcacgcc ggtgtaccgc gatttcttcc aggcgctcgg cgccacggtg
     5041 atgcagaccg cgccgggcga ggtgtacacc gcgctcgagc gcggcgtcgt cgacggctac
     5101 ggctggccga tcaatggcat cttcgacttc aactggcacg agaagaccaa gttccgcgtc
     5161 gacccgggct tctacaacgc cgaggtatcg ctggtcatga acctcgacaa gtggaaggcg
     5221 ctgaacagcg gacagcgcga tttgctcatg aagcacgtaa tcgcgctcga gaacgccaac
     5281 gactcgtgga agaaggtgaa cgaggacgac atccgcaagc agaaggaagc cggcatccag
     5341 gtgatcagct tcgacgccgc gactgcgaag cagtactacg ccaaggcgta tgaggtggcc
     5401 tgggaaaacg ccatcaaggc gagcccgcaa tacggtccgc agatgaggaa gctattcagc
     5461 aactagccga tggggtgcct cgcatccgcc tacggcaagg tgcttgccgc gcttgcgctg
     5521 cttgcctgcg cggtattgtt cgccatgatg ctggtgatct gcgtcgacgt gctgctgcgg
     5581 aacgttcggc tgattcccgg cgtctacagt gtggcatggg cgaacgaagc gaccgagtat
     5641 tcgctctacc tcatcacgct gctcaccgcg ccctggctgc tgcgccaggg tcagcatatc
     5701 cgcgtggacg tctttctgcg catagtgccg ccgcggatcg cctggtattg cgagtggctg
     5761 tgcgacctga cggcattcgt cgtttgcctc ctgatcgcct atgcctcctg gaaggccacc
     5821 ctgtcgagca tggcgatcaa tgccatggtg gtgaagacgc tcgcgctgcc cgaatggtgg
     5881 ctgcttgcgc cgatgcaact ctgcttcgtg ctcctcgcaa tcgaggtgct gtttcgcatg
     5941 gcccggctgc atgcgggcga gcgggggccg cgtgacgatg cggtcacggt ggcctgagcg
     6001 tgtcctggca ggcggcggcg tggctgatgc tgggcggctc gaccgccctg atgttcctcg
     6061 gcttgccggt ggcgttcgcg ttcctcgcga tcaatctgat cggcgccgcc atctacctcg
     6121 gcggcgagcc ggggctggtg cagttcgcgc gcaacggcgt gcagtcggta acgagcttcg
     6181 cgctcacgcc gatcccgctc ttcatcctca tgggcgaggt gctgttccat accgggctgg
     6241 cggtgaaggt gatcgacggc atcgagcggc tcatccgcca tgttccgggc agactggcag
     6301 tagtcgcggt cgtggcgggt accgtcttct ccgcgatttc cggctccacc atcgcgacca
     6361 ccgcgatgct cggcagcctc atgctgccgg tgatgctcgc gcgcggctat caccccacga
     6421 tctcgaccgg tcctatcatg gcgatcggcg ccgtggacat gctgatcccg ccctccgcgc
     6481 tcaccgtcct gctcggcagc ctctcgggta tctcgatctc caagctgctg gtcggcggca
     6541 tcttgcccgg gctggtgctg agcgtgatct ttatcgccta catcgtggtg cgcgtacgac
     6601 tcgagccgca gctcgcgccg aacacgccgt tcgaggagtt ccacggctgg cgcaggctgc
     6661 agccgttcgt gcagtacgtg ctgccgctgg tgtcgatctt cgtgatcgtg gtcggcgcga
     6721 tgatcgccgg ctgggccacg ccgaccgagt gcgcagcgct gggcgcgttc gcgacgattg
     6781 tgctcgccgt gctgtatcga gcgctgacgc tgcggaacct cgtgcgtgcc ctgcgcggca
     6841 ccgcgggcat ctccggcatg atcctcttca tcatcgtcgg cgcgaccacg ttctcgcaga
     6901 tcctgtcgtt ctccggcgcg agcagcggcc tcgcgcagat gatcacggac tcggggctgc
     6961 cgccgctcgc gatcatcgcg gggatgatgc tgttcctcat cttcctcggt atcttcgtcg
     7021 agcaggtgag catgatgatg atcacgctgc cgatcttcat gccgatcgtg cagca
//
LOCUS       DASTPD010000174         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1316028, whole genome shotgun sequence.
ACCESSION   DASTPD010000174 DASTPD010000000
VERSION     DASTPD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316028"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR57_05890"
     CDS             <1..258
                     /locus_tag="VFR57_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU0258933.1"
                     /translation="LLLAYPLHPAGKPEKLRDAHLAQIRMPVLCFNGTQDPLCRRDLM
                     EKALEKLSWQMYWLEGATHSFRVKDLKEVTEVSRAWLSRLA"
     gene            complement(227..1162)
                     /gene="senB"
                     /locus_tag="VFR57_05895"
     CDS             complement(227..1162)
                     /gene="senB"
                     /locus_tag="VFR57_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HEU0258934.1"
                     /translation="MRIALVTPAGPGTRNGNRHTALRWAVFLRQAGHRVSVSVQWSGD
                     ACDAMLALHAGRSHASIRSFPRNKPLVVALTGTDVYRDIHESADARESLELAHRLIVL
                     QPKAADELPARLRRKVRVVVQSSSTRLRQRPVNGGFRVCVIGHLRVEKDPLRMLSALE
                     LIPQSFRLQVIHLGAALDPALVPKSEDPRYRWLGSVPHARALKWLASSHAMVITSRME
                     GGANVVCEALRIGVPVLASRISGNVGLLGALYAGYFPVEDEHALARLVRRAATDPAYY
                     RTLKNQIAPLRKSVSPQAEARALLAVMRDATATRG"
     gene            1180..>2140
                     /locus_tag="VFR57_05900"
     CDS             1180..>2140
                     /locus_tag="VFR57_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0258935.1"
                     /translation="MTGLVETLKAAREHSRRVADDLGGEREFGPMLAIVNPPRWEVGH
                     VGWFQEYWCLRGGSEERPSILPNADALYNSATVPHDTRWSLPLPSFEDTIAYRDEVLE
                     RILAELESLEPYFVELAVRHELMHAEAFHYTRQTLGYPAPFPEKKISLKGEGDLECAG
                     GVFRLGASPGDGFAFDNEKWSHPIVLQPFRIARRPVTYGEYRRFAEPPCSRDGMVRRF
                     DKWIPIPNDEPVRHVSWHQANAHCAFVGRRLPSEAEWEYAALRGLDNVGHIWEWTTSA
                     FLPYPGFVRDPYKEYSEPWFATHKALRGASFATPAGVSHARFRN"
BASE COUNT          373 a          695 c          719 g          353 t
ORIGIN      
        1 ctgctgcttg cctatccgct gcacccggcg ggaaagccgg agaagctgcg cgatgcgcat
       61 ctcgcgcaga tccggatgcc cgtcctttgc ttcaacggca cccaagaccc gctatgccgg
      121 cgcgacctga tggaaaaagc gctcgagaag ctttcgtggc agatgtattg gctggaaggc
      181 gccacccatt cgttccgggt gaaagacctg aaggaagtga ctgaagtcag ccgcgcgtgg
      241 ctgtcgcgtc tcgcatgacg gcgagcaggg cgcgcgcctc cgcctggggc gagacgctct
      301 tgcgcagcgg cgcaatctga tttttcagtg ttcgataata ggcagggtcg gtggccgcgc
      361 gcctcacgag gcgggcgagc gcgtgctcgt cctccacggg aaaatagccg gcgtaaagcg
      421 caccgagcag gccgacgttg ccggagatgc gcgaggcgag caccgggacg ccgatgcgca
      481 gggcttcgca caccacgttt gcgccgcctt ccatgcgcga ggtgatgacc atggcgtggc
      541 tcgacgcgag ccatttcaag gcgcgcgcat gcggcacgct gccgagccag cggtaacgcg
      601 ggtcctcgct cttcgggacg agggctggat cgagggccgc tcccaggtgg atgacttgga
      661 gccggaagct ttgtggaatg agctcaagag ccgacagcat gcgcagcggg tccttctcga
      721 cgcgcagatg gccgatcacg catacgcgaa agccgccgtt caccggccgc tgacgaagtc
      781 gcgtggatga gctctgcacc acgacgcgga ctttcctgcg cagacgcgca ggcagctcat
      841 cggcagcctt gggctgaagg acgatgaggc gatgcgcgag ctccagcgat tcgcgcgcgt
      901 cggccgactc gtggatgtcg cggtacacgt ccgtgccggt aagcgccacc accagcggct
      961 tgtttcgcgg aaaactccgg atcgaagcgt ggctgcgccc tgcgtgcagg gcgagcatcg
     1021 catcgcaagc atcgcccgac cactggaccg agacgctgac gcgatggccg gcctgccgca
     1081 ggaaaaccgc ccagcgcagc gcggtatggc ggttgccgtt gcgtgtgccg gggccggccg
     1141 gcgtgacgag cgctattcgc attcgctaga ctggaacgcg tgacgggcct tgtcgagacc
     1201 ctgaaagccg cgcgtgagca cagccggcgc gtcgccgacg acctcggcgg cgagcgtgag
     1261 ttcgggccga tgctcgcgat cgtcaatcct ccgcgctggg aagtcgggca tgtcggctgg
     1321 ttccaagagt actggtgcct gaggggcggc tcggaagagc gcccgtcgat tctgccgaac
     1381 gccgatgccc tctacaactc ggccaccgtg ccgcatgaca cgcgctggag cctgccgctg
     1441 ccgtctttcg aagacacgat tgcatatcgc gacgaggtcc tggagcggat tctggccgag
     1501 ctggaaagcc ttgaaccgta cttcgtcgag ctcgccgtgc ggcacgagct catgcacgcc
     1561 gaggcgttcc actacacccg ccagaccttg ggctaccctg cgcctttccc tgaaaagaag
     1621 atttctctca aaggagaagg cgatctcgag tgcgcaggcg gcgtgtttcg cctcggcgcc
     1681 agtcccggcg acggctttgc gttcgacaac gagaaatgga gccaccccat cgtgctccag
     1741 cccttccgca ttgcgcgccg gccggtgact tacggcgagt accgccggtt cgccgagccg
     1801 ccttgctcca gggacgggat ggtgcgccgc ttcgacaagt ggatcccgat ccccaacgac
     1861 gagccggtgc ggcacgtgag ctggcaccag gcaaacgccc attgcgcctt cgtcggcagg
     1921 cgcctgccga gcgaagccga atgggagtac gcggcgctga gaggcctgga taacgttggc
     1981 catatctggg aatggaccac gagcgcgttc ttgccgtacc ccggcttcgt gcgcgacccg
     2041 tacaaggagt actccgagcc ctggttcgcc acccacaagg cgctgcgtgg cgcgagcttc
     2101 gccacgcccg ccggcgtatc gcatgcacgc ttccgtaact
//
LOCUS       DASTPD010000175         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_499381, whole genome shotgun sequence.
ACCESSION   DASTPD010000175 DASTPD010000000
VERSION     DASTPD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499381"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFR57_05905"
     CDS             complement(<1..423)
                     /locus_tag="VFR57_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0258936.1"
                     /translation="MELARLPVQRVALRLVEGIDSTLLIIILALSMLGFAALFSASYD
                     APGRVLSQLVSLGIALVAMWITAQIPPQTLMRFAVPAYVVGLVLLVMVALFGDVVNGA
                     RRWLHVGVTRFQPSEMMKLALPLMLAWYFHRRESTLRLR"
     gene            complement(423..2339)
                     /gene="mrdA"
                     /locus_tag="VFR57_05910"
     CDS             complement(423..2339)
                     /gene="mrdA"
                     /locus_tag="VFR57_05910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0258937.1"
                     /translation="MISAAELRNSERELQQFQLRIGIAGVAMLVAFGILLARFFFLQV
                     VQHDHYRAKAEDNRISIVPIAPNRGLIIDRNGVVVARNYSGYTLEIFPRRVRSVEETI
                     DQLSELIEITPRDRARFKRLAVETRNPESLPIRTRLSDEEVAKFAANRFRYEGVEIKA
                     RLFRQYPFGDVGSHVIGYMGRINQADQAALEQEGVDANYRGTDYIGKSGVEAAYEREL
                     HGTTGFEQVEIDAAGRGIRTLSHTPAVSGHNVLLTLDMQLQRVAENAFGSRRGALVAL
                     EPGTGAVLALVSKPGFDLNLFVDGIDPQAWTDLNNSPDRPLNNRAIAGVYPPGSTFKP
                     FMALAALELGKRRPSSTIHDPGYFVFGDRRFRDSKPGGHGVVDLYKSIVVSSDTYYYM
                     LANDMGIDAIAGFMKRLGLGSPTGIDLQGEAAGVLPSPEWKMKRFRRPEQQRWYPGET
                     ISIGIGQGYNAYTPIQLAQAMATLAANGEMYRPRVVGHIENPRTRERRSFEPELTSQV
                     PLKREWVDFVKRAMGGVNKEGTGARAFAKAEYTSGGKTGTAQVIGMKQNEKYDEAKVA
                     ERHRDHSLFIAFAPLEKPIIALAVIVENGGFGARAAAPVSRTVLDYYLVGKLPPGLQE
                     PTPEDEFDDGKESD"
     gene            complement(2336..2845)
                     /gene="mreD"
                     /locus_tag="VFR57_05915"
     CDS             complement(2336..2845)
                     /gene="mreD"
                     /locus_tag="VFR57_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU0258938.1"
                     /translation="MDLSGSRQILQPVRLSTIVVSFALALLLNFLPWRDLRLVPDFVA
                     LTLVFWCVRQPRLVGLGVGWTLGLITDAGNGVLLGQHALAYSLLAFLGIWLSRRILWF
                     GPALQALHVGAILLVGQGAALLVRLAAGDDFPGWQVFVSPLAGALLWPPTSWLLLLPQ
                     RRPAKEQTI"
BASE COUNT          505 a          960 c         1005 g          476 t
ORIGIN      
        1 gcgcagacgc agcgtcgact ctcggcgatg gaagtaccac gcgagcatga gcggcagcgc
       61 aagcttcatc atctccgagg gctggaagcg cgtcacgccc acgtgcaacc agcgccgcgc
      121 gccgttcacc acgtcgccga aaagcgcgac catcacgagc agcaccaggc caacgacata
      181 cgccggcacg gcgaagcgca tcagcgtctg cggcgggatc tgcgcagtga tccacatggc
      241 gacgagcgcg ataccgaggc tcacgagctg gctaagcacc cggcccggtg cgtcgtagct
      301 tgcggagaac agcgccgcga agccgagcat ggaaagcgcc aggatgatga tgagaagcgt
      361 gctgtcgatg ccttcgacga gtcgaagggc gacgcgctgg acgggcaggc gcgcgagttc
      421 catcagtcgc tttccttgcc gtcgtcgaac tcgtcctcgg gcgtgggctc ctgcagcccc
      481 gggggcagct tgccgacgag gtagtagtcg agcaccgtgc gcgacaccgg tgccgccgcg
      541 cgggcgccga agccgccgtt ctcgacgatc acggcgagcg cgatgatcgg cttctcgagc
      601 ggcgcgaacg cgatgaacag cgaatgatcg cgatggcgct cggcaacctt ggcctcgtcg
      661 tacttctcat tctgcttcat gccgatgacc tgcgcggttc cagtcttgcc gccgctcgtg
      721 tactcggcct tggcgaacgc gcgcgcgccg gtgccttcct tgttgacccc ccccatggcg
      781 cgcttgacga aatcgaccca ctcccgcttg agcggtacct gcgaagtgag ctcgggctcg
      841 aagctgcgcc gctcgcgagt gcgggggttc tcgatatggc cgactacccg cggccggtac
      901 atctccccgt tggcggcgag cgtcgccatc gcctgcgcga gctggatcgg tgtgtaggcg
      961 ttgtacccct ggccgatgcc gatggagatc gtctcgcccg ggtaccagcg ctgctgctcg
     1021 ggccggcgga agcgcttcat cttccattcc ggcgagggca gcacccccgc ggcttctccc
     1081 tggaggtcga tgccggtcgg gctgccgagc ccgagccgct tcatgaaccc ggcgatggcg
     1141 tcgatgccca tgtcgttcgc gagcatgtag taatacgtgt ccgaggacac gacgatcgac
     1201 ttgtagaggt cgaccacccc gtgcccgccc ggcttggagt cgcggaaacg ccggtcgccg
     1261 aatacgaagt agcccgggtc gtggatagtg ctggacggcc gacgcttgcc gagctcgagc
     1321 gcggcaagcg ccatgaaagg cttgaacgtc gagcccggcg ggtatacccc tgcgatggcg
     1381 cggttgttga gcggtcggtc cggtgaattg ttgaggtcgg tccaggcttg cgggtcgatg
     1441 ccgtcgacga agaggttaag gtcgaatccc ggcttggaca ccagggcgag tactgcgccg
     1501 gtgcctggct cgagcgccac cagcgccccg cggcggcttc cgaaggcgtt ctcggcaacg
     1561 cgctgcagct gcatatcgag cgtgagcaag acgttgtggc cggacactgc gggcgtgtgc
     1621 gacagcgtgc ggattccgcg gccggcggcg tcgatctcga cctgctcgaa gccagtggtg
     1681 ccgtgcagct cgcgctcgta ggccgcctcg accccgctct tgccgatgta gtcggtgcca
     1741 cggtagttgg cgtcgacgcc ctcctgctcg agggccgcct ggtcggcctg gttgatgcgc
     1801 cccatgtagc cgatgacatg cgagccgacg tcgccgaacg ggtactggcg gaacagccgc
     1861 gccttgatct cgacgccttc gtagcggaag cggttggcgg cgaacttggc cacctcttcg
     1921 tcgctcaacc gcgtgcggat cggcaggctc tcgggattgc gcgtttccac cgcgagccgc
     1981 ttgaagcgcg cccggtcccg cggcgtgatt tcgatgagct ccgacagctg atcgatggtt
     2041 tcctcaacgc tcctcacgcg gcgcggaaag atctcgagcg tgtagccgga atagttgcgc
     2101 gccacgacaa cgccgttgcg gtcgatgatc agcccgcggt tgggcgcgat cggaacgatg
     2161 gagatacggt tgtcctcggc tttcgcccgg tagtggtcgt gctgcactac ctgcaggaag
     2221 aagaagcgcg cgagcagaat gccgaaagca acgagcatcg ccacgccggc gatgccgatg
     2281 cgcaactgga actgctgcag ttcgcgctcg gaattgcgca actccgcggc gctgatcata
     2341 tggtctgctc tttcgcgggg cggcgctgag gaagcaggag cagccaactc gtcggtggcc
     2401 agaggagcgc cccggcgagc gggctgacga agacctgcca gcccggaaaa tcgtcgccgg
     2461 cggcgaggcg cacgaggagg gccgcgccct ggcctacaag caggatcgcg cccacgtgga
     2521 gcgcctgcaa cgccggaccg aaccacagaa tgcggcgcga cagccagatg cccagaaacg
     2581 cgagcagcga gtatgcgagc gcatgctgcc cgagcagcac gccattgccc gcgtcggtga
     2641 tgagcccgag cgtccaaccg actcccaggc cgacgagccg cggctggcgc acgcaccaga
     2701 aaaccagcgt gagcgcgacg aagtccggca ccaggcgcag gtcgcgccac ggcaggaagt
     2761 tgagaaggag cgccagcgcg aacgagacga cgatggtgct cagccgaact ggctgaagga
     2821 tctgacggct acctgacaga tccattgccg tccctcaccc gcccgcggcg cgtgcgctcc
     2881 tttgttgtct ccatttcttc gggacgcggc ggcttgactg tctcatcgga gagcacgagc
     2941 acgaag
//
LOCUS       DASTPD010000176         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_704887, whole genome shotgun sequence.
ACCESSION   DASTPD010000176 DASTPD010000000
VERSION     DASTPD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704887"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFR57_05920"
     CDS             <1..485
                     /locus_tag="VFR57_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0258939.1"
                     /translation="RLERLLPGPVERVWAYLTESEKRATWLCGGEFELRVGGRVRLEF
                     DHARLSREKATPERHKGAEHARFEGTITRLEPLRALAFTWTFADRLSEVTFELAPSGK
                     QVLLTITHRGLDERATRVSVMSGWDVHTGILADVLNGTEPRPFWSTHARLEREYEARI
                     "
     gene            complement(501..890)
                     /locus_tag="VFR57_05925"
     CDS             complement(501..890)
                     /locus_tag="VFR57_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258940.1"
                     /translation="MEVLLVDDQAVTIQVYAASVRKTFSGAKVHTAVDLPEALQIAAG
                     RTLDLVLLDLSLPTCTGIEALYRFRSAFPAVPILVVSANEDRQRVLECLQAGARGYIT
                     KTSAVQGLPAAMKVVVEGGKYVPPRIH"
     gene            complement(1472..3145)
                     /locus_tag="VFR57_05930"
     CDS             complement(1472..3145)
                     /locus_tag="VFR57_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0258941.1"
                     /translation="MTVETVIRGALVVDGTGGPPKRADIGVGAGKILEVGNISTGGRK
                     TIAAAGLVLAPGFIDPHTHYDAQICWDGATTPSSWHGVTTVVMGNCGVGIAPCRPAAR
                     EIAMRDLVNVEAIPFEVLNAGITWDWESFPEYMDAAARRKPALNLAFLAPLTPFRHYA
                     MGEASMERAANGSEIQKIESLLVEAIDAGAFGWSSTLLNQHMGYGARPLACRNASREE
                     IKAYCNVLRERRKGAIEVAMTRQISVLEQAELELLDFMLKESCRPVTLIAMFDRDDLP
                     EALRTTMRKLQPMIARGARPQTSPLPLTRELVMRSPFAFAAFPSWKRLFEDTSKEAQA
                     AVYRDPLFRKQFREDLKRPASFADWARITVHEMKNPKLKHLESKTVAEIAKERGVDGV
                     DALLDVTLEDALENEFTVQSWNTRIDRMTELLNDRSVLLGLGDGGAHLDMLCDSGYPT
                     YVLGTWVRERKVLSLEEAVRRMTSDPADFFGIKDRGRIAPGMAADLVLFDPASVGMAT
                     RPERIHDLPAGGKRMVKRSSGIEMTMVNGVVTWERGALTGSAAGTVLRS"
     gene            3181..>4011
                     /locus_tag="VFR57_05935"
     CDS             3181..>4011
                     /locus_tag="VFR57_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0258942.1"
                     /translation="MSLPRRMACLDFDALYRAESRRVFATLVRLLGDFDLAEEALSEA
                     FRAALEQWPREGVPANPRAWLVSAGRFKAIDAIRRQARFDVLDEEKLENLEAETHEVH
                     EIEDDQLRLIFTCCHTALSPDARVALTLREVCGLKTEEIAHAFLTPAPTLAQRIVRAK
                     IKIRDARIPYEVPGPGELAGRLEAVLRVIYLVFNEGYSASSGSSVTRAELSQEAIRVG
                     HLLVELLPEPEAMGLLALMLLHESRRAARTSANGDLILLEDQDRGKWDAQLIAEGVTL
                     V"
BASE COUNT          672 a         1352 c         1272 g          715 t
ORIGIN      
        1 tgcgcctgga gcgcctgctc cccggcccgg tggaacgcgt gtgggcttac ctcaccgaat
       61 cggagaagcg cgcgacgtgg ctctgcggcg gggaattcga gctgcgggtc ggcggcagag
      121 tacggctcga attcgaccat gccaggcttt cgcgcgagaa agcgacaccc gagaggcata
      181 aaggcgccga gcacgcacgc ttcgaaggaa cgattacgcg ccttgagcca ctccgcgcgt
      241 tggcgttcac ctggaccttc gccgatcggc tctcagaggt gacattcgag ctcgcgccga
      301 gcggcaagca ggtgctgctt accatcaccc accgcgggct ggatgagcgt gccacccggg
      361 tgagcgtcat gagcggctgg gacgtgcaca ccggcatcct cgccgacgtg ctgaacggaa
      421 ccgagccccg ccccttttgg agcacgcacg cgcggctcga gcgcgagtac gaagcgcgaa
      481 tctagacgga acgacctcgc ctagtgaatc cggggtggga cgtacttccc gccctccacc
      541 acgaccttca tcgccgcggg caggccctgc accgcggagg tcttggtgat atagccgcgt
      601 gctccggcct ggaggcactc gaggacgcgc tgccggtcct cgttggccga gacgacgaga
      661 atcggaacgg cgggaaacgc gctccggaag cgatagagcg cttcgatgcc ggtgcaggtc
      721 ggcagtgaaa gatcgagcag cacgaggtcg agcgtgcgcc cggccgcgat ctgcagcgcc
      781 tcgggaagat caacggcggt gtgcaccttg gcaccgctga acgtcttgcg cacgctcgca
      841 gcgtagacct ggatggtgac cgcctggtcg tccaccagca gcacttccat gtcgggcatc
      901 ttagaccccc cctgtctaac gccggtcgag cagttccgct tttgtcgttc ttttgccggt
      961 cgcactgcag cattcttgcc ttaccacacg aagcggcagc ctcacaccca gagggacaga
     1021 ttttgccccc tgtggacaag ccaaggcctc agaccgggcg cgccagcctg acgccctgcc
     1081 ttcacactga tgtgtggctt acttgctcgt cggcgaagat ggcgacgacg ggctgctcgg
     1141 cgacgtgctg cgcttctggt tcgtctggcc atttgtgccg cctgcgcccg acgagtcact
     1201 tgagctgccc gtcgcgcccg tgcctccgct acccgcaggg cgatcgcagc cggcgagaag
     1261 cgcgccggag agcgcgaggc taagggcgag ggtcagtgtg cgtgtcagtg tgcctgtcat
     1321 gtgtggctct cctttcgggc cggaacaagc aacaagcacg ccgattcccc gccgccgcgc
     1381 cgactgtttt gcggagctcg aaagcttggt agacgggatg gtgaagcggc gctagggaaa
     1441 aactactgca cgatgtgctg accccttttc ttcaggagcg caggaccgtc ccggcggctg
     1501 atcccgtcag cgcgcctctc tcccacgtca ccacgccgtt taccatggtc atttcgattc
     1561 cgctcgagcg cttgaccata cgcttgccgc ccgcgggcag gtcgtggata cgctcggggc
     1621 gcgtcgccat gccgacggag gcgggatcga agagtacgag gtcggctgcc atgcccggcg
     1681 caatgcgccc gcgatccttg atgccgaaga agtcggccgg atcggacgtc atgcgccgta
     1741 cggcttcttc cagcgacagc actttccttt cgcgcaccca ggttcccagc acgtacgtcg
     1801 gataacccga gtcgcacagc atgtcgaggt gcgcgccgcc gtcccccagc ccgaggagga
     1861 cgcttctgtc gttgagcagc tcggtcatgc ggtcaatgcg cgtgttccag gactgcacgg
     1921 tgaactcgtt ttcaagggcg tcctcgaggg ttacgtcaag caacgcgtcg acgccatcca
     1981 ctccccgctc tttggcgatc tccgccaccg tcttgctctc cagatgcttc agcttcggat
     2041 tcttcatctc gtggaccgtg atgcgcgccc agtcggcgaa cgacgcggga cgcttcagat
     2101 cttcacggaa ctgcttccgg aacagaggat cccggtagac ggcagcctgc gcttccttgg
     2161 aggtgtcctc gaagagccgc ttccacgacg gaaacgcggc gaacgcgaac gggctgcgca
     2221 tcaccagctc ccgcgtgagc ggaagcggcg aggtctgcgg ccgcgcgccg cgcgcgatca
     2281 tgggctgcag cttgcgcatc gtggtgcgca gcgcctccgg caggtcgtcg cggtcgaaca
     2341 tcgcgatcag cgtcaccggc cggcagctct ctttgagcat gaagtcgagc agctcgagct
     2401 ccgcctgctc cagcaccgag atctgccgcg tcatcgcgac ctcgatcgcg cccttgcgcc
     2461 gctcgcgcag cacgttgcag taggccttga tctcctcgcg gctcgcgttg cggcacgcga
     2521 gcggtcgcgc cccatagccc atgtgctggt tcaacagcgt gctcgaccaa ccgaacgctc
     2581 cggcatcgat tgcttcgacc agcaaggatt ctattttttg aatttcagaa ccatttgcgg
     2641 ccctttccat cgaggcttcg cccatcgcgt agtgccgaaa cggcgtgagc ggcgcgagga
     2701 aagcaaggtt gagcgcgggc ttgcgccgcg ccgcggcatc catgtactcc gggaaacttt
     2761 cccagtccca tgtgatgccg gcgttcagaa cctcgaacgg aatcgcttca acgttgacga
     2821 gatcccgcat tgcgatctcc cgtgccgcag gccggcacgg cgcgatgccg accccgcagt
     2881 tccccatcac cacggttgtc acgccgtgcc aggaggacgg cgtcgtcgcc ccgtcccaac
     2941 agatctgcgc atcgtagtgg gtgtgcggat cgatgaagcc cggcgccaac accagccctg
     3001 cggcagcgat ggttttccga ccaccagtcg agatgttgcc gacctcaagg atctttccgg
     3061 caccgacgcc gatatcggcg cgcttgggcg gaccaccggt tccgtccaca acgagcgcgc
     3121 cccggatcac ggtttcaact gtcatggtcg gcgatcgtag cagcagtcct ccatctggta
     3181 atgtcgttgc ctcgccggat ggcctgcctc gacttcgacg ccctgtatcg cgctgagtcg
     3241 cgccgggttt tcgccacgct cgtgcgcctg ctcggcgact tcgacctcgc ggaagaagcg
     3301 ctctcggagg cgttccgcgc cgcgctcgag cagtggccgc gcgagggcgt gcccgccaac
     3361 ccgcgcgcct ggctggtctc ggccgggcgc ttcaaggcaa tcgatgccat ccgccgtcag
     3421 gcccgctttg atgttctgga cgaggaaaag ctcgaaaacc ttgaagcgga aacacacgaa
     3481 gttcatgaaa tagaagacga ccagctgcgg cttatcttta cgtgctgcca tacggcgctc
     3541 tcgcccgacg cgcgagtcgc gctcacgctg cgcgaagtct gcggcctgaa gaccgaagag
     3601 atcgcgcacg cgttcctcac gccggcaccg acgctcgcgc agcgcatcgt acgcgccaag
     3661 atcaagatcc gcgacgcgcg catcccctac gaggtaccgg gtcccggcga gctcgccggg
     3721 cggctcgagg cggtgttgcg cgtcatctac ctcgtcttca acgagggcta ttctgcgtct
     3781 tccggcagct cggtgacgcg cgccgagctt tcgcaggaag cgatccgggt cggccacctg
     3841 ctggtcgagc ttctgcccga gcccgaggcg atggggctgc tcgcgctgat gctgctgcac
     3901 gaatcgcgcc gcgccgcgcg cacctccgcg aacggcgacc tgatcctgct ggaagaccag
     3961 gatcgcggca aatgggacgc gcagcttatc gccgagggcg tgacgctggt g
//
LOCUS       DASTPD010000177         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_296156, whole genome shotgun sequence.
ACCESSION   DASTPD010000177 DASTPD010000000
VERSION     DASTPD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296156"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFR57_05940"
     CDS             complement(<1..1127)
                     /locus_tag="VFR57_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0258943.1"
                     /translation="MRALVPALAILVLTGCASLSDDARFGAVEQAVKERTGVDTKWTR
                     SDDEAESVRGRVKELLAKPLGPTEAVQIALLNNPGLQASYAEVGIAEADLVQASRWRG
                     PTFSFSRLRRGDEVEIERGVFFDVLGLLTIPLSTRASESRLAATQHRAAAEALRIALE
                     TRKAWFHAVAAQETAKYSAQVREAAETSAELARRMAEVGNFPKLTYAREQAFHADAIA
                     QLARARHNATATRERLARLMGLWGEDLAFQLPDRLPDLPKAPREGGDLEAQAIAQRLD
                     VQGARRETESVARSLGLTKVTRFVDLLEFGIQDKKETGEEKWRGWELELGIPIFDFGG
                     ARVARAERLYMQAVNRTIETAVEARSEVRETYSAYRTAFDLA"
     gene            complement(1124..1411)
                     /locus_tag="VFR57_05945"
     CDS             complement(1124..1411)
                     /locus_tag="VFR57_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258944.1"
                     /translation="MRLILGVVPFIGLAACAADRTAVPPSAREAQAVVPLVEYRSAFA
                     DYKPFADQELADWRKANDEVGAAGGHAAHKPGQGPAQQTSKPEPGKPGGHQ"
     gene            complement(1489..1923)
                     /locus_tag="VFR57_05950"
     CDS             complement(1489..1923)
                     /locus_tag="VFR57_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258945.1"
                     /translation="MALKGADVELGAMAKVSRILAIALLTVTLPLQGLAGVVSAQCMV
                     IGHHADAGLAGHTHVEGGEADSHDHAGHSHGEEDAAEQSTDGTKASHCGPCTACCTSA
                     SIASAAALVILPSLSPTLYVFSQLAPPAVVPDGVDRPPLSLS"
     gene            complement(1984..2481)
                     /locus_tag="VFR57_05955"
     CDS             complement(1984..2481)
                     /locus_tag="VFR57_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258946.1"
                     /translation="MKSRKTRALRIMLKVLAIGMSLAFFAAFAAHIYRLDFKPMAGVG
                     VPVLLVFFGFASLFFIRGRSLAKGSAQFRSLVAAERAVQAAVWHLSGIMLSNVLYTVL
                     MRSGGMVDASERWMLAAWVLLFLVPHALMQIGLFSFMRAVVVVAPHLFRKVGAFELRR
                     RVALS"
     gene            complement(2574..2696)
                     /locus_tag="VFR57_05960"
     CDS             complement(2574..2696)
                     /locus_tag="VFR57_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258947.1"
                     /translation="MIKLICGAAAAVALIVAAFIAFTMEHHWKGTDTVRPPGAS"
     gene            complement(2718..3203)
                     /locus_tag="VFR57_05965"
     CDS             complement(2718..3203)
                     /locus_tag="VFR57_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEU0258948.1"
                     /translation="MEHVHHAHHQHHARQPSLNRTAWSATLHCLTGCAIGEVLGMVIG
                     TALGWGNGATIALAVALAFLFGFALTLLPLLRATGFNTALKLALASDTASITVMEIVD
                     NAIMLVIPGAMDAGLGTLLFWGSLAFALGVAAIAAFPINRWLIARGKGHAVVHAYHHH
                     H"
     gene            complement(3214..3381)
                     /locus_tag="VFR57_05970"
     CDS             complement(3214..3381)
                     /locus_tag="VFR57_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258949.1"
                     /translation="MATIQVVLGLLCLTHPLLPGVGVSLAACAIGLGLIVSGLLAGVD
                     DEEAHDLDFSR"
     gene            3481..4518
                     /locus_tag="VFR57_05975"
     CDS             3481..4518
                     /locus_tag="VFR57_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0258950.1"
                     /translation="MDSPETLADIAIFAAVIREGSFTRAADKLELSTSQVSKCVNRLE
                     RNLGARLLHRTTRRLRLTEAGSALYENSSTALAAIEDAKLAVSKLQGAPRGKLKVSTS
                     TAFGTMQLPRVIRDLSAQYPELEVELVLEDRHVDLVREGIDVAIRITGEAPDSGLVYR
                     KLAPNRQVICASPDYIGRRGLPRTPDDLANHNCIAHSARTTPRIWHLTGPDGGQISVP
                     INGRPTINSALAVRQAALEGLGIIELNSYLVGEDIAAGRLARLLPQYQPKELNFYAVY
                     AERRFLAPKIRVFVDAMLARMSPEPPWDSFLNLPEKTRIERTPASTAEDATIRQGYGR
                     KNQPGNRARRR"
     gene            complement(4400..4714)
                     /locus_tag="VFR57_05980"
     CDS             complement(4400..4714)
                     /locus_tag="VFR57_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0258951.1"
                     /translation="MTKIVYVNERDVAAREIYPGVRKRVLWQGPNGAKAQLLEIDAGA
                     KFLKLDVHHPGPKEIYVVSGTFNDGARDYPAGSFVHNPAGSSHLPQSRQGCVLFVFFP
                     EG"
BASE COUNT          948 a         1590 c         1536 g          814 t
ORIGIN      
        1 gccagatcga aggcggtgcg ataagcgctg taggtctcgc gcacctcgga gcgagcctcg
       61 acggccgtct cgatcgtccg gttcaccgcc tgcatgtaca ggcgttcggc gcgcgcgacc
      121 cgcgcaccgc cgaagtcgaa gatcggaatg ccgagctcga gttcccagcc gcgccacttc
      181 tcctcgccgg tttccttctt gtcctggatg ccgaactcga gcaagtccac gaagcgcgtc
      241 accttcgtca gcccgagcga acgggcgacc gattcggttt cgcgccgggc accctgcaca
      301 tcgagtcgct gggcgatcgc ttgcgcctcg agatcgccgc cttcgcgagg cgctttcggc
      361 agatccggca gccggtcggg aagctggaac gcgaggtcct cgccccacaa gcccatcagt
      421 cgcgcgagcc gctcgcgcgt cgccgtggcg ttgtggcgcg cgcgcgcgag ctgagcgatg
      481 gcatcggcat ggaaggcctg ctcgcgcgcg taggtgagct tgggaaagtt gccgacttcg
      541 gccatgcggc gcgcgagctc tgcgctagtc tcggctgcct cgcgcacctg cgccgagtac
      601 ttcgcggtct cctgcgccgc gaccgcatgg aaccaggcct tgcgcgtctc cagcgcgatg
      661 cgcagcgctt cggccgccgc gcggtgctgt gtggcagcga gacggctttc gctcgcacgc
      721 gtcgaaagcg ggatagtgag aagcccgagc acgtcgaaaa aaacgccgcg ctcgatctcg
      781 acttcgtcac cgcgccgcag ccggctgaac gagaacgtcg gtccgcgcca gcggctcgcc
      841 tgcacgagat ccgcctcggc gatgccgacc tctgcgtagc tcgcctgcag gccgggattg
      901 ttcaggagcg cgatctgcac cgcctcggtg ggcccgagcg gcttggcgag caactccttc
      961 acacggccgc ggacgctctc tgcctcatcg tcgcttcgcg tccacttggt gtcgacgccg
     1021 gtgcgctcct tcacggcctg ctcgaccgca ccgaagcgtg cgtcgtcgct caggctcgcg
     1081 caccccgtga gaacgagaat cgcgagcgct ggtacgagcg ccctcattgg tgacctcccg
     1141 gcttgccggg ttccggcttg gaggtctgct gagcgggtcc ttgccccggc ttgtgggccg
     1201 cgtggccgcc ggccgcgcca acctcgtcgt tcgccttgcg ccagtcggcg agctcctggt
     1261 cggcaaacgg cttgtaatcc gcgaacgcgg agcggtactc caccagcggt acgacggcct
     1321 gcgcctccct agcgctcggc gggacggcgg tccggtcggc cgcacaggca gcaagcccga
     1381 tgaagggcac tactccaaga atgagtcgca tagctctctc gaaagcggtt ttcgcgcacc
     1441 taaaagtgcg ccaaagagcg gcgcttgctg gcgcgccgcg cctaagccct acgagagaga
     1501 aagcggtggg cgatctacgc cgtcgggcac aacggccggc ggcgccaact gcgagaacac
     1561 gtacagcgtg ggggaaagcg aaggcaaaat gacgagcgcc gcggcactcg cgatggaagc
     1621 cgaggtacag cacgccgtgc aaggtccgca atgcgaggcc ttagtaccgt cggtgctttg
     1681 ctcggctgca tcttcctcgc cgtgagaatg accggcatgg tcgtgactgt ccgcttcgcc
     1741 gccttcgacg tgcgtatgac cggctaggcc cgcatccgcg tgatgcccga tgaccatgca
     1801 ttgcgcggac acgacacccg cgagcccctg cagcggcagg gtaacggtca gcagcgcaat
     1861 cgcaagaatg cgggaaactt ttgccatcgc tcccaattct acgtcagccc ctttgagcgc
     1921 caagggcaca cgcagttccc gcggccgtgc gcccccggag ataggagacc cgtgaggaca
     1981 ttgtcacgaa agggcgacac ggcgccgcag ttcgaacgct cccactttgc ggaacaagtg
     2041 cggtgcgacg accacaacgg cacgcatgaa actgaaaaga ccgatctgca tcagcgcatg
     2101 cggcacaagg aacaacagca cccaggcagc gagcatccag cgctcgctgg cgtcgaccat
     2161 gccccccgag cgcatcagca cggtatagag aacattgctg agcatgatgc cggacaggtg
     2221 ccacaccgct gcctggactg cccgctctgc ggcgacaagc gagcggaatt gcgcactccc
     2281 cttcgcaagg gaccgaccgc gtatgaagaa aagcgaggcg aagccgaaga acacgagcag
     2341 aactggaacg ccgacacccg ccatcggctt gaaatcaagc ctgtagatgt gagccgcgaa
     2401 tgcggcgaaa aaagcgagcg acattccgat ggcgagaacc ttcaacatga tgcgcagggc
     2461 gcgcgtttta cgcgacttca tagtcaatcc ccaagtgtcg gattagtggg tgcggaaaga
     2521 aacttcgccg gcgccgaaac gccgccgcca agctgcgcag gaacgcctcg agctcaagaa
     2581 gctcccggcg gacggacggt gtcagtaccc ttccagtgat gctccatcgt aaacgcgatg
     2641 aacgcggcga cgatcaaagc gaccgcggca gcggcaccac aaatcaactt gatcatggtc
     2701 gtcttccttt tccggaatca atggtggtgg tgataggcat ggaccaccgc gtggcccttg
     2761 ccgcgggcga tcagccagcg attgatcggg aacgccgcga tagcggcgac gccgagtgcg
     2821 aaagcgaggc tgccccaaaa gagcagtgtg cccagaccgg catccatcgc ccccgggatg
     2881 accagcatga tcgcgttatc cacgatctcc atcacggtga tggaagcggt atcggacgcg
     2941 agggcgagct tcagggccgt gttgaatccg gtcgccctga gtaacggcag aagcgtcagc
     3001 gcaaatccga acaggaaggc gagcgccacg gcgagtgcga tcgtggcgcc gttgccccag
     3061 ccgagcgcgg taccgatgac catgccgagc acttcgccga tggcacagcc agtcaggcag
     3121 tgaagcgtcg ctgaccacgc ggtgcgattc agcgacggct ggcgcgcatg gtgctggtga
     3181 tgcgcgtgat gaacatgttc catgtcgatt tccttatctc gagaagtcca ggtcgtgggc
     3241 ttcctcgtcg tcgacgccgg cgagaagccc gctgacgatg aggcccaggc cgatggcgca
     3301 tgcggcgagg ctgacaccga cgccgggaag cagcgggtgg gtgaggcaga gcagtccgag
     3361 taccacctgg atcgtcgcca tgtgtgcccc tcctttcgat cgaaccggtg tgaattgttc
     3421 gcccgcgcgc cgcggagatc atctggcgat ggggaaattc tttatgcaca atcggaaaca
     3481 atggacagcc cggagacgct ggccgacatc gcgatcttcg ccgcggtaat acgcgagggc
     3541 agctttacgc gcgccgcaga caaactggag ctctcaacct cgcaggtcag caagtgcgtc
     3601 aaccggctgg aacgtaacct cggcgcccgg ctgctgcacc gcacgacgcg ccggcttcgt
     3661 cttaccgagg ccggctcggc gctctacgag aacagctcga cagcgctcgc cgccatcgaa
     3721 gacgcgaagc tcgccgtatc caaacttcag ggtgcaccac gtggcaagct gaaggtcagc
     3781 acctcaactg cgttcggcac gatgcagctc ccccgggtca ttcgcgacct gagcgcgcag
     3841 tacccggagc tcgaggtcga gctggtactg gaggaccgcc acgttgatct cgtcagggag
     3901 ggaatcgacg tcgcgatccg gattacgggc gaggcgcccg actcggggct ggtctatcgc
     3961 aagctcgcgc ccaaccggca agtgatttgc gcctcgccgg actacatcgg acgccgtgga
     4021 cttccgcgta cgcccgacga cttggcgaac cacaactgca tcgcccacag cgcccgcacc
     4081 accccgcgta tatggcatct cacgggtccg gacggcgggc agatttcagt gccgatcaac
     4141 ggccgaccca ccatcaatag cgcgcttgcc gtccgccagg ccgcgcttga agggctcggc
     4201 atcatcgagc tgaattcata tctggtcggc gaagacatcg cggcagggcg gctcgcacgg
     4261 ctgctgcctc agtatcaacc gaaagagttg aacttctacg ccgtctacgc cgaacgtcgt
     4321 ttccttgctc ccaagatccg ggtgtttgtc gatgccatgc tggcccgtat gtcgcccgag
     4381 ccgccgtggg attcgttcct caaccttccg gaaaaaacac gaatagaacg cacccctgcc
     4441 tcgactgcgg aagatgcgac gatccggcag ggttatggac gaaagaacca gccgggtaat
     4501 cgcgcgcgcc gtcgttgaac gtgccggaaa cgacataaat ctccttcggc cccgggtgat
     4561 gaacgtcgag cttcaggaac tttgcgccgg catcgatctc gaggagttgc gccttagcac
     4621 cattgggccc ttgccatagc acgcgtttgc gcacgcccgg atagatctcg cgtgcggcca
     4681 cgtccctctc attgacgtaa acgattttgg tcatcccctc attgtctcga tatcctcggc
     4741 cgcgatcatt tgcaccccga aaatcacttt gcctcgatat gcataatggt cgggaatcct
     4801 aagcggggca atgcgtagag caccgcgctg aggatgggaa gataatgaaa gcaacgccgc
     4861 gttgaacggg cgcaattact actgatgt
//
LOCUS       DASTPD010000178         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_705362, whole genome shotgun sequence.
ACCESSION   DASTPD010000178 DASTPD010000000
VERSION     DASTPD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705362"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFR57_05985"
     CDS             complement(<1..579)
                     /locus_tag="VFR57_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEU0258952.1"
                     /translation="MSEKSSGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREETASDKGGKGAKSDLPTPREIRNILDQYVIGQDQAKKILSVAVYNHYKRLKHLS
                     KNDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVENI
                     IQKLLQNCDYDVDKAKRGIVYIDEIDKISRKSD"
     gene            complement(580..1203)
                     /gene="clpP"
                     /locus_tag="VFR57_05990"
     CDS             complement(580..1203)
                     /gene="clpP"
                     /locus_tag="VFR57_05990"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU0258953.1"
                     /translation="MNWEKSSDVRDLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLV
                     GPVTEVTANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGLAIYDTMQFIKPDVSTL
                     CVGQAASMGALLLAAGDAGKRFALPNSRVMIHQPMGGFQGQATDIEIHAKEILYLRER
                     LNEILAKHSKQPIDVIRRDTERDFFLGAEDAVKYGLVDKVLTSRENT"
     gene            complement(1200..2489)
                     /gene="tig"
                     /locus_tag="VFR57_05995"
     CDS             complement(1200..2489)
                     /gene="tig"
                     /locus_tag="VFR57_05995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU0258954.1"
                     /translation="MANVETLGTLERRVSVAVAADEVERKVDERLKQLARNVRMPGFR
                     PGKVPLKLIAQTYGPQVRSEVLSDAVQKSFTDAVKEAKLRVAGYPKFENTKDLEFSAT
                     FEIYPEVKVGDLAAATIERPQVEVDDAAVDKTLEILRKQRTRFEPAARPAQDDDRLTI
                     DFRGTIDGQPFAGGEAKDFAFALGGGRMLPEFEAAARGMSAGEEKTFELTFPADYHGK
                     EVAGKKASFTMTLKKIDAPVLPELDAEFAKTLGVADGDVAKMRAEVRANVEREVKKRV
                     EARLKDQALDALLAATPLEVPKALVGMEAASMAERAAADLKGRGIDPTQVPINPQAFE
                     DTARRRVALGLIINELKQSENVNPKPAEVRAMVEQEAQSYESPAEVVKWFYMQPQRLS
                     EMEAMALEGNVVKWVLAKAKVLDKPVTFDELMGATGS"
     gene            complement(2502..2586)
                     /locus_tag="VFR57_06000"
     tRNA            complement(2502..2586)
                     /locus_tag="VFR57_06000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2550..2552),aa:Leu,seq:tag)
     gene            2629..>3026
                     /locus_tag="VFR57_06005"
     CDS             2629..>3026
                     /locus_tag="VFR57_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU0258955.1"
                     /translation="MGVGHYENFPVASLLVPAPLRAPIETIYRFARSADDFADEGDDA
                     PEIRLARLNDYRTQLAAPASPLFEELRKIIREHELPAGLFADLLDAFAQDVTKKRYAN
                     FAELLDYCRRSANPVGRLLLHLFKRTSDSNL"
BASE COUNT          455 a         1014 c          944 g          613 t
ORIGIN      
        1 gtccgacttg cgcgagatct tgtcgatctc gtcgatgtag acgatgccgc gcttcgcctt
       61 gtcgacgtcg tagtcgcagt tctgcagcag cttctggatg atgttctcga cgtcctcgcc
      121 gacgtagccg gcctcggtga gggtcgtcgc gtccgcgatc acgaacggca cgttgaggag
      181 gcgcgccagc gtctgcgcga gcagcgtctt gcccgagccg gtcggcccga tcagcaggat
      241 gttcgacttg gcgagctcga cctcgtcgtt cttggacagg tgcttgagcc gcttgtagtg
      301 gttgtagacc gcgaccgaga ggatcttctt ggcctggtcc tggccgatca cgtactggtc
      361 gaggatgttg cggatctcgc gcggcgtcgg caggtcggac ttggcgccct tgccgccctt
      421 atcggaggcg gtctcctcgc ggatgatgtc gttgcacagc tcgatgcact cgtcgcagat
      481 gaacaccgac gggccggcga tgagctttct tacctcgtgc tggctcttgc cgcagaacga
      541 gcagtaaaga agcttttccc ccgagctctt ttccgacact tacgtgttct ccctggacgt
      601 cagtaccttg tccacgagac cgtactttac cgcgtcttcg gcgccgagga aaaagtcgcg
      661 ctccgtgtcg cgccggatca cgtcgatcgg ctgcttgctg tgcttggcca ggatctcgtt
      721 cagccgctcg cgcaggtaga ggatttcctt cgcgtggatc tcgatgtcgg tggcctggcc
      781 ctggaagccg cccatcggct ggtggatcat gacgcgcgag ttgggcagcg cgaagcgctt
      841 gccggcgtcg ccggcggcga gcagcagcgc gcccatgctg gcggcctgcc ccacgcacag
      901 cgtcgacacg tcgggcttga tgaactgcat ggtgtcgtag atcgcgagcc ccgccgacac
      961 cgagccgccc ggcgagttga tgtagaagaa gatgtccttg tccgggttct cggactcgag
     1021 gaacaggagc tgcgcgacga tcaggttggc ggtcacctcg gtgaccggac cgaccaggaa
     1081 caccacgcgt tccttgagca gcctggagta gatgtcgtag gcgcgctcgc cgcgcccgga
     1141 ctgctcgatg accatcggga tcaacccgag gtcgcgcacg tcagaagact tttcccagtt
     1201 cacgagcccg ttgcccccat cagctcgtca aacgttaccg gcttgtcgag cacttttgcc
     1261 ttcgccaaaa cccatttgac gacgttgcct tccagcgcca tggcttccat ctccgaaagc
     1321 cgctgcggct gcatgtaaaa ccattttact acctcggcgg gactctcgta gctttgcgcc
     1381 tcctgctcca ccatcgcgcg cacttccgcc ggcttgggat tcacgttttc cgactgcttg
     1441 agctcgttga tgatcagccc gagcgcgacg cggcggcgcg cggtgtcctc gaaggcctgc
     1501 gggttgatcg gcacctgcgt cggatcgatg ccgcgcccct tgaggtcggc ggcggcgcgc
     1561 tctgccatgc tcgcggcctc catgccgacc agggccttcg gcacctcgag cggcgtggcg
     1621 gcgagcagcg cgtcgagcgc ctggtccttg agccgcgcct ccacacgctt cttcacctcg
     1681 cgctcgacgt tcgcgcgcac ctcggcgcgc atcttggcga cgtcgccatc ggcgacgccg
     1741 agcgtcttgg cgaactcggc gtcgagctcc ggcagcaccg gcgcgtcgat tttcttcagc
     1801 gtcatggtga aggaagcctt tttcccagca acttccttgc catggtagtc ggcggggaaa
     1861 gtgagctcga aggtcttctc ctcgcccgcc gacatgccgc gcgccgcggc ctcgaactcg
     1921 ggcagcatgc gcccgccgcc gagcgcgaac gcgaagtcct tcgcctcgcc gccggcgaac
     1981 ggctggccgt cgatggtgcc gcggaagtcg atggtcagcc ggtcgtcgtc ctgcgcgggc
     2041 cgcgcagccg gctcgaagcg cgtgcgctgc ttgcgcagga tctcgagggt cttgtcgacc
     2101 gccgcgtcgt ccacctcgac ctgcggacgc tcgatcgtgg ccgcggcgag gtcgcccacc
     2161 ttcacctccg gatagatctc gaaggtcgcg ctgaactcga ggtccttggt gttctcgaac
     2221 ttcgggtagc ccgcgacgcg cagcttcgct tccttcacgg cgtcggtgaa ggacttctgc
     2281 accgcgtcgc tcagcacttc ggagcgcacc tgcgggccgt aggtctgcgc gatgagcttc
     2341 agcggcacct tgcccggccg gaagcccggc atgcgcacgt tgcgcgcgag ctgcttcagt
     2401 cgctcgtcca ccttgcgctc gacctcgtcg gctgccaccg cgaccgatac gcggcgctcg
     2461 agcgttccca gcgtttcaac gttggccatg tcttttcttc gtggtgcgaa gggagggact
     2521 cgaaccctca tgccttgcgg cgtcagatcc taagtctggt gcgtctgcca attccgccac
     2581 cttcgcggct gccaccttct ctaaataatc aacgtattat agccgccgat gggggtgggc
     2641 cattacgaga acttcccggt cgcgtcattg ctcgttccgg cgccgctgcg cgcgccgatc
     2701 gaaacgatct accgcttcgc gcgcagcgcg gacgatttcg ccgacgaagg cgacgacgct
     2761 cccgagatac gcctcgccag gttaaacgac taccgcacgc agctcgccgc gcccgcctcg
     2821 ccgcttttcg aggagctgcg gaaaatcatt cgcgaacatg agcttccggc cgggctgttc
     2881 gccgacctgc tcgacgcctt cgcgcaggac gtgacgaaga agcgctatgc aaattttgcc
     2941 gagctgctcg attactgccg ccgctcggcg aacccggtcg ggcggctgct gctgcacctg
     3001 ttcaaacgaa cgagtgattc aaatct
//
LOCUS       DASTPD010000179         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1521747, whole genome shotgun sequence.
ACCESSION   DASTPD010000179 DASTPD010000000
VERSION     DASTPD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521747"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFR57_06010"
     CDS             complement(<1..376)
                     /locus_tag="VFR57_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258956.1"
                     /translation="MLLLAALIAWVAPLLGLAVSGNPITPYLAFPPRTLPATHAPASW
                     LWFCLMSVPAIAGLGLIVLAVARARPQTDVVEARRAFPLWGWLGLATLAAGWFAAWSE
                     HTPAEWRRHAFTPLWVGYVLAVN"
     gene            complement(549..1310)
                     /locus_tag="VFR57_06015"
     CDS             complement(549..1310)
                     /locus_tag="VFR57_06015"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0258957.1"
                     /translation="MRLKDKVALITGAGSGIGRQTALLFSREGAAVACVDVNEAAARE
                     TASQLKNSIPVKADVSKARDCEQMVAAAEKAFGKLNVLFNNAGIMHANDDDAVSTSEE
                     VWDLTLDINAKGVFLGCKYGIPALKRAGGGSVINTASFVAKMGAATPQIAYTASKGAV
                     LSMTRELAVIHARQNIRVNALCPGPLKTELLMSFLNTEAKKQRRLVHIPMGRFGEAEE
                     IAKAALFLASDESSYVTGTEFLVDGGITGAYVTPE"
     gene            complement(1307..2686)
                     /locus_tag="VFR57_06020"
     CDS             complement(1307..2686)
                     /locus_tag="VFR57_06020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU0258958.1"
                     /translation="MSKTRGMLTLEQLKGMVAQGEIETVLAGFTDHYGRLMGKRFDAE
                     MFVDEVAKHGGHACDYLLTVDMEMEPVPGYSFANWELGYGDVHMVPDFGTLRLASWLE
                     KTAFILCDVKSDKTQDYVNVAPRSVLRRQIAAARKMGYAGYAGTELEHYLFRTSYRQA
                     AQQDYRNLEPAGWYLEDYHMLQGMRTEGFHAAARRHLKLSGVPVENSKGEWGKGQHEL
                     NVRYAEALEMADRHVVFKQCLKEIAEAQGLSVTFMAKFAADSAGSSCHIHFSLWRDGK
                     NAFPGNAALGPVKASDTFRYFLGGWIAHVPDVMVFYAPTINSYKRYVDASWAPTRLAW
                     SHDNRTAGFRVVGEGQSLRIECRIPGADCNPYLALAASLASGLEGIANKIEPPECFIG
                     DIYQAKNLPRVPYTLADANEKFASSAFCRKAFGEAAVEHYSHFYRTEAQAYDKAVTDW
                     ERRRYFERI"
     gene            complement(2679..2957)
                     /gene="yhbY"
                     /locus_tag="VFR57_06025"
     CDS             complement(2679..2957)
                     /gene="yhbY"
                     /locus_tag="VFR57_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU0258959.1"
                     /translation="MLTPAERKVLKARAHKLEPVVQLGAKGLTDEVIAEVDRALRAHE
                     LIKVRAGGLDRDQRDKALDSICRSTNAEEVQHVGKVFVLFRKNENADE"
     gene            2985..>3628
                     /locus_tag="VFR57_06030"
     CDS             2985..>3628
                     /locus_tag="VFR57_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0258960.1"
                     /translation="MFALLPPPHLPSDFHPVLQHWWASRFDAPTAAQLEGWRAIREGR
                     HTLIAAPTGSGKTLAAFLTSIDELLKEGIEQGGLPDEVRVIYVSPLKALSADIHKNLA
                     VPRREIHALSPVKITAAVRSGDTPQPERAAMLRTPPHILVTTPESLYLLLTAERSRAM
                     LKTARAVIVDEIHAVLQSRRGAHLALSLERLDHVCGRKLQRIGLSATQKPIDEVA"
BASE COUNT          619 a         1190 c         1150 g          669 t
ORIGIN      
        1 cgttcaccgc cagcacatag ccgacccaca acggcgtgaa agcgtggcgg cgccactcgg
       61 ccggagtgtg ctcgctccac gcggcgaacc atcccgccgc gagcgtggcg agcccgagcc
      121 agccccaaag agggaaggca cgtcgtgcct ccacgacgtc cgtctgcggg cgcgcccgtg
      181 ccactgcgag gacgatgagg ccgagccctg ctatagcggg gaccgacatc aggcagaacc
      241 agagccacga agccggcgcg tgcgtagccg gaagagttct cggcggaaac gcgagatacg
      301 gcgtaatcgg attgccgctt accgcgaggc ccaaaagcgg cgccacccag gcgatcagtg
      361 ctgcgagcag cagcacccag agcgcccgca gcctcgtgtc gtcaggcgac ggcgacaatc
      421 accgtgacaa ggacgaggcc gatggaatag agccagtaat cgagcgtact catgcccgtg
      481 atgctcatgc gggcagatga gatcccgatt tccgacggcc tccttccttt tacaaatgaa
      541 accaacggct actcgggcgt cacgtaggcg ccggtgatgc cgccgtcgac aaggaactcg
      601 gtgccggtga cgtaggagga ctcgtccgac gcgaggaaga gcgccgcctt ggcgatctcc
      661 tcggcctcgc cgaagcggcc catgggaatg tggaccaggc ggcgctgctt cttcgcctcg
      721 gtgttcagga agctcatcag cagctcggtc ttgagcggcc cggggcacag ggcgttcacg
      781 cggatgttct ggcgggcgtg gatgacggca agctcgcgcg tcatggagag cacggcccct
      841 ttgctcgcgg tgtacgcgat ctggggcgtc gccgcgccca tcttggcgac gaacgaggcg
      901 gtgttgatca ccgacccgcc gccggcgcgc ttcagtgccg gaatgccgta cttgcagccc
      961 aggaagacac ccttagcgtt gatgtcgagc gtgaggtccc agacctcttc gctcgtcgac
     1021 acggcatcgt cgtcgtttgc gtgcatgatg ccggcgttgt tgaagaggac gttgagcttg
     1081 ccaaatgctt tttcggccgc ggcgaccatc tgctcacagt ctcgggcttt cgacacatct
     1141 gcttttactg gaattgaatt cttgagctgt gaagccgtct cgcgagcggc cgcctcgttg
     1201 acgtcgacgc aagccacagc cgcgccttcc cgcgaaaaga ggagggcggt ttgccggccg
     1261 atgccgctgc ccgcgccggt gatcagcgcc actttgtctt tcagtctcat atgcgttcga
     1321 aataccgcct gcgttcccaa tcggtaacgg ccttgtcgta ggcctgcgcc tcggtgcggt
     1381 agaagtggct gtagtgctcg acggccgctt cgccgaatgc cttcctgcaa aacgcactgg
     1441 aggcgaattt ttcgttcgcg tccgcgagcg tatacggcac gcgcggcaag tttttcgcct
     1501 ggtagatatc gccgatgaag cactcgggcg gctcgatctt gttggcgatg ccctcgagcc
     1561 ccgacgcgag ggacgcggcg agcgcaaggt acggattgca gtccgcgccg gggatgcggc
     1621 actcgatgcg caggctttgg ccttcgccca cgacgcggaa gcccgcggtg cggttatcgt
     1681 ggctccacgc gaggcgcgtc ggcgcccacg aggcgtccac gtaacgcttg tacgaattga
     1741 ttgtcggcgc gtagaacacc atcacgtcgg gcacgtgcgc gatccagccg ccgaggaaat
     1801 atctaaacgt atcggaagct ttcaccgggc cgagagctgc gttcccgggg aaggcattct
     1861 ttccgtcgcg ccagaggctg aagtggatat ggcagctcga gccggcactg tcggcggcga
     1921 acttggccat gaaggtgacg ctcagccctt gcgcctcggc gatctccttc aggcactgct
     1981 tgaacaccac atggcggtcc gccatctcga gcgcctcggc gtagcgcacg ttgagctcgt
     2041 gctgaccctt gccccattcg cccttggagt tctcgaccgg cacgccggaa agcttgaggt
     2101 gccggcgcgc cgcggcgtga aacccttcgg tgcgcatgcc ctgcagcatg tggtagtcct
     2161 cgaggtacca gccagcgggc tcgaggttgc ggtagtcctg ctgagcggcc tggcggtagc
     2221 tcgtccggaa gaggtagtgc tcgagctcgg tccccgcata gcccgcgtag cccatctttc
     2281 tcgccgcggc gatctgccgt cgcaggaccg agcggggcgc aacgttgacg tagtcctgcg
     2341 tcttgtcgct tttcacgtcg caaaggatga acgccgtctt ctccagccag ctcgccagcc
     2401 ggagcgtgcc gaagtcggga accatgtgca cgtcgccata gccgagctcc cagttggcga
     2461 agctgtagcc cggcacaggc tccatctcca tgtctacggt taggagatag tcgcaggcgt
     2521 ggccgccgtg cttggcgacc tcgtcgacga acatctcggc gtcgaagcgc ttgcccatga
     2581 gccggccgta gtggtcggtg aatccggcga gcacagtttc gatctccccc tgcgccacca
     2641 tccctttgag ctgttcgagc gtcagcattc cgcgcgtctt actcatcggc gttctcgttt
     2701 ttccggaaaa gcacgaagac ctttccgacg tgctggactt cttctgcgtt ggtgctccgg
     2761 caaatcgaat caagcgcctt gtcgcgctgg tcgcgatcca ggcccccggc gcgcaccttg
     2821 atgagctcgt gggcgcgcag cgcgcggtcg acctccgcga tcacttcgtc ggtaaggccc
     2881 ttcgcgccga gctgcaccac cggctcgagc ttgtgagccc ttgccttcag caccttgcgt
     2941 tcggccggcg tcagcatgaa tgaatcccgg aatctaatgt caaaatgttc gccttgctac
     3001 ccccgccgca tttgccttcc gacttccatc ccgtcctgca gcactggtgg gcgagccgtt
     3061 ttgacgcgcc caccgcggcc cagctcgaag gctggcgcgc gatccgcgaa gggcgccaca
     3121 cgctcatcgc cgccccgacc ggctcgggca agacgctcgc ggcttttctg acgtcgatcg
     3181 atgagctgct caaagaggga atagagcagg gcggactgcc ggacgaggtg cgcgtcatct
     3241 atgtctcccc gctaaaggca ctctcggcgg acatccataa gaacctcgcc gtgcctcggc
     3301 gggaaatcca tgcgctgtca ccggtgaaaa tcacagcggc agtccgtagc ggcgacacgc
     3361 cgcagcctga gcgcgctgcg atgctgcgaa ccccaccgca cattctggtg acgaccccgg
     3421 aatcgcttta tctgctcctt accgcggagc gctcacgtgc gatgctcaag accgcgcgcg
     3481 cggtcatcgt ggacgagatc cacgccgtgc tccagtcgcg ccgcggcgcg catctcgcgc
     3541 tttcgctcga gcgcctggac cacgtgtgcg ggcgcaagct gcagcgcatc gggctgtccg
     3601 ctacgcagaa gcccatcgac gaagtggc
//
LOCUS       DASTPD010000180         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_297152, whole genome shotgun sequence.
ACCESSION   DASTPD010000180 DASTPD010000000
VERSION     DASTPD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297152"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="VFR57_06035"
     CDS             complement(<1..2643)
                     /locus_tag="VFR57_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0258961.1"
                     /translation="MKAPAKRRAWAAKRPAKQEVDRFELAMRAINEGIYEWNIADGST
                     VYSDRVLRSLGLSRRQLRNAADWHQRIHPEDRARFDAALRDHFKGRTERFECDLRYRA
                     GDGGWRWARQHGVAIRDAKGRAVRMIGSTGDITELKRVEEALKASEERYALATRAATE
                     GIYEWNLECDSLYLSDRAKAFFAVAGEALTPAAWNARVHGEDFQGYRDAIVAFFKGRK
                     PVFEHEYRIRDAAGGYAWVRDRAIAVRDPAGRVTRFVGALTDITQRRRAELELRRAHE
                     STTEALARQTATAEILKAIAASPTDVRPVLEALARHAGELCGAAYVNVLLEEESMLRT
                     MAVYSAAGGPPADTAFAMRALPTTVNGRAFVERRPVHVPDFMAVAATEYPDSLEFQRH
                     FGFRTILGVPMLRAGQAIGTISIWRREVRPFSAEEIALIETFAAQAVIAIENVRLFNE
                     TKEALEQQTASSEILRVISSSPADLAPVFDAILGGAMQLCEAHLGTLNLYDGEWLHTV
                     AQRGGNPQFAKWLLERGAIKPDGGMVHQAITERRGVQIADAREAPGYREGKPNISKFV
                     DLGAVRTFLAVPLLKDSAVIGIIGIYRPEVRPFSEKQIALVSMFANQAVIAIENARLF
                     NETREALERQTATAEILKVIARSPADVQPVFDAIVTAALKLCAATSANAVTFDGELIH
                     VAAVAEAGASAAEALGRHFGTYPKRPSSDTANTRAILTRQVVAIPDVMEDAEYAAKAT
                     AVAAGYRSIVSVPLMRGQNPIGALTVARPEPGHFPERKLALLRTFADQAAIAIENARL
                     FNETKEALEQQTATTNILHVISRSVTDLAPVFEAILENAITLCEGNVAALWQYDGTHL
                     RFAAGKRVSPEATAHYRSHPLALGT"
     gene            complement(2640..3671)
                     /locus_tag="VFR57_06040"
     CDS             complement(2640..3671)
                     /locus_tag="VFR57_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258962.1"
                     /translation="MTQHRILVVDDTPANVKLLSDLLSVKGYQVSTAVNGEQALDKVA
                     KEKPDLVLLDVMMPGMSGYEVCKRIRANPETALLPVVMCTSLDPNQERVKGIEAGADD
                     FLSKPVNQPELFARVRSLLRIKGLQDERLGRLKSFFSPQLAEAIAAGKGEELLKTHRR
                     EITVEFFDLRGFTHFTEGAEPEEVMELLRAYHSALGKIVLEHQGTLERFAGDAVMVFF
                     NDPMPIEKPAEHAVRTAMAMHEAFMPIGKHWEKRGFELALGVGIAQGYATLGAIGFEG
                     RWDYAAIGNVTNLAARLCGEAKAGQVLLDRKTMAALDGAFECDPVGPLTLKGFSQAVP
                     AFALRQPAG"
     gene            complement(3668..4042)
                     /locus_tag="VFR57_06045"
     CDS             complement(3668..4042)
                     /locus_tag="VFR57_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258963.1"
                     /translation="MSTILIIEDNEKNMKLARDVLQAKGYKTLEAVTGEDGVKLAKEN
                     KPDLVLMDIQLPGINGIEAFKQIRGDAATKGIPVIALTASVTPTDRTAITAAGFDAFL
                     GKPINLKEFLETVRRLLEKSRT"
     gene            complement(4039..>4824)
                     /locus_tag="VFR57_06050"
     CDS             complement(4039..>4824)
                     /locus_tag="VFR57_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0258964.1"
                     /translation="FADQAVIAVENARLFNEIRDKSRQLEVANKHKSEFLANMSHELR
                     TPLNAIIGFSEVLSERMFGELNEKQADYLKDIHESGKHLLSLINDILDLSKIEAGRMD
                     LEISTFDLPSALSNAMVLVRERAQRHGIDLTLEVDKELGAFRADERKFKQIVLNLLSN
                     AVKFTPDGGRVSVAAKKDTTHVEIAVKDTGIGIAPEDQAAVFEEFKQVGRDYTKKAEG
                     TGLGLALTKRFVELHGGDIRLESALGTGSTFTISLPLTPPSIQ"
BASE COUNT          736 a         1574 c         1607 g          907 t
ORIGIN      
        1 ggtgcctagt gcgagcgggt ggctgcgata gtgggccgtc gcttcggggc ttacccgctt
       61 gcctgccgcg aagcgcagat gagtgccatc gtactgccaa agcgcggcga cgttgccttc
      121 gcacagagtg atcgcgtttt cgagaatcgc ctcgaaaacg ggagcgagat cggtcacgga
      181 acggctgatg acgtgcagga tattcgtggt cgccgtctgc tgctccagtg cctcctttgt
      241 ctcgttgaac aagcgcgcgt tctcgatcgc gatggccgcc tggtcggcga aggttcggag
      301 caacgcaagc ttcctctccg gaaagtgccc cggctcgggc cgtgccaccg tgagcgcgcc
      361 gatcggattc tggccgcgca tcaacggcac cgacacgatg ctgcggtagc cggcggccac
      421 ggcagtcgcc ttggcggcat actcggcgtc ttccatcacg tccgggatcg cgaccacctg
      481 gcgagtcagg atggcgcgcg tattcgcggt gtcggaacta ggccgtttcg ggtaggtgcc
      541 gaaatgccgg ccgagcgcct ccgcggcgct ggcgcccgcc tcagccaccg cggcaacgtg
      601 aatcagctcc ccatcgaagg tgacggcatt cgcggaggta gcggcgcaaa gcttcaacgc
      661 agcggtgacg atcgcgtcga acaccggctg cacgtcggcc ggcgagcgcg cgatcacctt
      721 caggatctcc gcggtcgccg tctggcgctc gagcgcctcc ctggtctcat tgaagaggcg
      781 ggcgttttct attgcgatca ccgcctggtt cgcaaacatg ctgacaagcg cgatctgctt
      841 ctcgctgaaa ggacggacct ccgggcggta gatgccaatg atgccgatta ccgcgctgtc
      901 ctttaggagc ggcaccgcga ggaaggtgcg cactgcgccc aggtccacga acttgctgat
      961 gttcggcttg ccttcgcggt atccgggggc ttcgcgggca tcggcgattt gcaccccccg
     1021 gcgttcggtg atggcttgat ggaccatccc gccgtcgggc ttgatggcgc cccgctcgag
     1081 cagccatttc gcgaattgcg gattgccgcc ccgctgggcc acggtatgaa gccattcccc
     1141 gtcgtacaga ttgagcgttc ccagatgcgc ctcgcatagc tgcattgcgc cgccgaggat
     1201 ggcatcgaaa acgggagcga ggtcggccgg cgagctgctg atgacccgca agatctcgct
     1261 gctcgccgtc tgctgctcaa gcgcttcctt cgtctcattg aacaggcgca cgttctcgat
     1321 ggcgatgact gcctgtgccg cgaaggtctc gatcagcgcg atttcctcgg cgctgaacgg
     1381 acgcacctcg cgccgccaga tggagatggt gccgattgcc tgccccgcgc ggagcatcgg
     1441 tacgccgagg atggtgcgga agccgaagtg gcgctggaac tccagcgagt cggggtactc
     1501 ggtcgcggcc acggccatga aatccggcac gtgaaccggc cggcgctcga cgaaagcgcg
     1561 gccgttcacc gtcgtgggca gcgcgcgcat ggcgaacgcg gtgtcggccg gcggcccgcc
     1621 ggccgcggag tacaccgcca tggttcgcag catgctttcc tcttccagga gaacgttcac
     1681 gtacgcggcg ccgcacaact cacccgcgtg gcgcgccagc gcctcgagga ccggccgtac
     1741 gtcggtcggt gacgcggcga tcgctttcag gatttccgcg gtagcggtct gccgcgcgag
     1801 cgcctcggtg gtcgactcgt gcgcccggcg cagctcgagt tcggcgcgcc ggcgctgggt
     1861 gatgtcggtc aatgcaccga cgaagcgcgt gacgcgccct gcgggatcgc gcaccgcaat
     1921 cgcgcgatcg cggacccaag cgtagccgcc ggccgcgtcg cgaatgcggt actcgtgctc
     1981 gaagacgggc ttgcgtccct tgaagaacgc cacaatggcg tcgcggtaac cctggaagtc
     2041 ctcgccgtgg acgcgtgcgt tccacgccgc cggcgtgagc gcctcgcccg ccaccgcgaa
     2101 gaaggccttc gcacggtcgg aaaggtagag cgagtcgcac tccaggttcc attcatagat
     2161 gccctcggtc gcggcgcgtg tggccagcgc gtagcgttcc tcgctcgcct tgagcgcctc
     2221 ctcgacgcgc ttgagctcgg tgatgtcgcc ggtggagccg atcatgcgca ccgcgcggcc
     2281 tttcgcgtcg cggatagcga cgccgtgctg gcgcgcccag cgccacccgc catcgcccgc
     2341 gcggtagcgc aagtcgcact cgaagcgctc ggtgcggccc ttgaaatggt cccgcagcgc
     2401 cgcgtcgaag cgcgcgcggt cctccggatg gatgcgctgg tgccagtcgg cggcattcct
     2461 gagttgccgg cgcgacagcc ccagggagcg cagcacgcgg tccgagtaga cagtgctgcc
     2521 atcggcgatg ttccactcgt agataccctc gttgatcgct cgcatcgcga gttcgaagcg
     2581 atccacttcc tgctttgccg ggcgcttggc cgcccaggcc cggcgcttgg ccggcgcttt
     2641 catccggccg gctggcgcag cgcgaaggcc ggcaccgctt gcgagaagcc cttcagcgtg
     2701 agcgggccga ccgggtcgca ctcgaaggcg ccgtcgagcg cggccatggt cttgcggtcg
     2761 agcagcacct gcccggcctt ggcctcgccg cacaggcgcg cggcgaggtt ggtcacgttg
     2821 ccgatcgccg cgtagtccca gcggccctcg aagccgatgg cgccgagcgt ggcgtagccc
     2881 tgggcgatgc caaccccgag cgccagctcg aaaccgcgct tttcccagtg cttgccgatc
     2941 ggcatgaatg cttcatgcat tgccatggcg gtgcgcacgg cgtgctcggc cggcttttcg
     3001 atcggcatgg ggtcgttgaa gaagaccatc accgcgtcgc cagcgaagcg ctcgagtgtg
     3061 ccttggtgct cgagcacgat cttccccaaa gccgagtggt aggcgcgcag gagctccatg
     3121 acttcctccg gctccgcgcc ttcggtgaaa tgggtgaagc cgcgcaggtc gaagaactcg
     3181 accgtgatct cgcggcgatg cgtcttcaag agctcctcgc ccttgccggc ggcaatggcc
     3241 tcggcaagct gcggcgagaa aaagctcttc aggcgcccaa ggcgctcgtc ctgcaatcct
     3301 ttgatgcgaa ggagcgagcg cacgcgcgcg aagagttccg gctggttgac cggcttcgac
     3361 agaaagtcgt ccgcgcccgc ttcgatgccc ttcacgcgct cctggttcgg atcgagcgag
     3421 gtgcacatca cgaccggtag cagcgcggtc tccgggttcg cgcggatgcg cttgcacact
     3481 tcgtaccccg acatgccggg catcatcacg tcgagcagca cgaggtcggg cttttccttc
     3541 gccaccttgt cgagcgcctg ctcgccgttc accgcggtcg aaacctggta gcccttcacc
     3601 gacagcaggt cggagagcag cttcacgttt gccggcgtgt cgtcgacgac gaggatgcgg
     3661 tgttgcgtca tgtcctcgac ttctccagca gtcgtctcac cgtctcgagg aactccttga
     3721 ggttgatcgg cttgccgagg aaggcgtcga acccggcggc ggtaatcgcg gtgcggtcgg
     3781 ttggcgtcac tgaagcggtc agcgcgatta cgggaatgcc tttcgtcgcc gcgtcgccgc
     3841 ggatctgctt gaaagcctcg atgccattga tcccggggag ttggatgtcc atgaggacga
     3901 ggtcgggctt gttctccttg gcaagcttca cgccgtcctc gccggtcacc gcctccagcg
     3961 tcttgtagcc cttcgcctgg agcacgtccc gggcgagctt catgttcttt tcgttgtctt
     4021 cgatgatcag gattgtgctc attggatgga tggaggcgtg agtgggagcg agatggtgaa
     4081 cgtggatccc gtgccgaggg cgctctcgag gcggatgtcg ccgccgtgca gctcgacgaa
     4141 gcgcttggtg agcgcgaggc cgagccccgt gccctcggcc ttcttggtgt agtcgcgccc
     4201 gacctgcttg aactcctcga acaccgcagc ttggtcctct ggcgcgatgc cgatgccggt
     4261 gtccttcacc gcgatctcga cgtgcgtcgt gtccttcttc gccgccacgg acacgcgccc
     4321 gccatcggga gtgaacttga ccgcgttgga gaggaggttg agtacgatct gcttgaattt
     4381 gcgctcgtcg gcccggaacg cccccagttc cttgtcaacc tcgagcgtga gatcgatccc
     4441 gtgccgctgg gcgcgctccc gcacgagcac catcgcgttc gagagcgccg agggcaggtc
     4501 gaaggttgag atctcgaggt ccatgcgccc ggcctcgatc ttcgagaggt cgaggatgtc
     4561 gttgatgagc gagagcaggt gcttgcctga ctcgtggatg tccttgaggt agtcggcctg
     4621 cttctcgttc agctcgccga acatgcgctc ggagagcacc tcggaaaagc cgatgatcgc
     4681 gttcaagggc gtcctcagct cgtgcgacat attggcgagg aattccgact tgtgcttgtt
     4741 ggccacttcg agctggcggc tcttgtcccg gatctcgttg aagaggcgcg cgttctctac
     4801 cgcgatcacg gcctgatccg caaa
//
LOCUS       DASTPD010000181        11863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1522496, whole genome shotgun sequence.
ACCESSION   DASTPD010000181 DASTPD010000000
VERSION     DASTPD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522496"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFR57_06055"
     CDS             <1..617
                     /locus_tag="VFR57_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0258965.1"
                     /translation="YNPEGVTAYNIVPGLLGTILTMTMVLMTGLAMTRERERGTFENL
                     LSTPALPIEVMTGKIVPYILIGLIQVTLIILCALWLFTVPMQGSYLLLYLVVLVFIAA
                     NLTLGITFSSIARNQLQAMQMTFFFFLPSILLSGFMFPFRGMPPWAQWLGSALPLTHF
                     VVLVRGIMLKGSGLADLWPQIWPILVFMLVVIAIGLRFYKRTLD"
     gene            614..1249
                     /locus_tag="VFR57_06060"
     CDS             614..1249
                     /locus_tag="VFR57_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0258966.1"
                     /translation="MKAEFRDRDAAGTRLAAMLGKYRGGSAIVLAIPAGGEPVGAAIA
                     RDLGLSLRAAPVSKMLYPWTTESGFGAVAFDGSAWIDQAAVEGYGLTSSQVTKATADA
                     RAKVERRSKKLSSANALRDLSGKTVILVDDGIAAGSTMRTAIDALRKLGAGRVVVAVP
                     TAPARSLEAMRKLADEIVCADIRSGASFAVADAYEEWRDLSDDEVEAMLRP"
     gene            1246..2115
                     /locus_tag="VFR57_06065"
     CDS             1246..2115
                     /locus_tag="VFR57_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU0258967.1"
                     /translation="MTPVIAIHGGAGVLHADKPGAHHRAVLKQALDAGYEILKRDRSS
                     LDAVAAAVVVLEDSPLFNAGRGSSFNMDGEIEMDASIMDGATLRAGAVAAVRRIRNPI
                     LAARAVMEKSRHVLLAGSGAERFARKQGLKFELPEYFHTARRRTALKKNLRNYHGTVG
                     AVALDVDGNLAAATSTGGYTGKMPGRVGDSPLVGAGTYADNRACAVSGTGLGEAFIRA
                     AVAYDVCARMRYAGATLAAAAADALKAVAALGADGGVIAVDRRGSVAMPFNSDGMFRA
                     CIDRRGRRTIAIF"
     gene            2112..2516
                     /locus_tag="VFR57_06070"
     CDS             2112..2516
                     /locus_tag="VFR57_06070"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEU0258968.1"
                     /translation="MKVELRYAPLPWIGAIAWHYWFVVCDDTGRHRWEVWQTKNAGGW
                     CIGHVHRDLKHPDDGVGGGPSRLVREWDGDHAVKILRVLQTIESYPYCERYRYWPGPN
                     SNTFVAWVLREAGIDYKLSRKGLGQGYLTAKQ"
     gene            complement(2495..3112)
                     /locus_tag="VFR57_06075"
     CDS             complement(2495..3112)
                     /locus_tag="VFR57_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258969.1"
                     /translation="MKTLLTTIGLLVALSAQAQQKDMSFFITSVGPGKGADLGGLAGA
                     DAHCQKLAQTAGAGQREWRAYLSTDKVNARDRIGKGPWQNAKGEEVAKSVDDLHKDPK
                     IDKQTGLDEKGNMVNGRGDSPNRHDILTGSTPDGRAIADKDLTCGNWTKSGEGAAMVG
                     HHDRVGLRDDDASKSWNSSHPSRGCSQDALRSSGGDGLFYCFAVR"
     gene            complement(3140..4231)
                     /locus_tag="VFR57_06080"
     CDS             complement(3140..4231)
                     /locus_tag="VFR57_06080"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HEU0258970.1"
                     /translation="MKTFWIVLFALACGAARAAEPSQFIFAWPLDEKALKPRGATTRG
                     APVTLDAAPTESWKRLNEPGLGAQERDRRAILAMAGPYRVSFDFLEVVRYEPSLKQDA
                     PYQSWGTEYVFVTEDRPEFIALQHILVMRMLRDGGSGGSPIVSEPMVVRHWRQEWRYE
                     APTLLAYEGENTWARRATPAAARRGAWSQTVLQVDDSPRYAALGRWEHSDGVSTWISD
                     ETWRPLPRREFSVRKDYDVLVGTNRHTITPTGWVQEENNLKLALKDRKYLAREYGVAR
                     YQRIRDYDFGAGEKYYARTEPFWTEVRAAWRELEEGRGRFTVRAPVDQAQLFLPFFRY
                     ADQLVEGAPFDRDDARAFVRRTLQDSYLK"
     gene            complement(4228..5301)
                     /locus_tag="VFR57_06085"
     CDS             complement(4228..5301)
                     /locus_tag="VFR57_06085"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0258971.1"
                     /translation="MSAPPFRAEHIGSLLRPQRLLQDRSCADECIVEAIRWQESLGLK
                     VVTDGEFRRESWRLGFVSKVAGFARADAVGNVDLQRDAAGNVMRVGSAPVAVTRIRRT
                     GPIVADEVGFSLKHTHRVVKATLPAPSYLHYPRGRDCVDRDVYPDLDEFFADVVKVYV
                     EELHALHAAGGRYLQIDEVAQTLLCDQKLRDAVRARGDDPEKLIDLYIDLINRVVRER
                     PAGMTVGVHMCRGNAMGKWIAEGGYERIAEKSFSRMEVDAFFLEFDSERAGGFEPLRF
                     VPNGKRVVLGLVSTKTPALEDKDALKEKIDAAARLIPLDSLSISPQCGFASHEKGTML
                     SFRDQEAKLRRVVETAMEVWGQA"
     gene            complement(5298..9428)
                     /gene="treY"
                     /locus_tag="VFR57_06090"
     CDS             complement(5298..9428)
                     /gene="treY"
                     /locus_tag="VFR57_06090"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEU0258972.1"
                     /translation="MADEVASSYLDVWGNEKSIEPEVRRVLLEAMGPRRRARKVRLAT
                     GRCYQPKLLEERRLWGFMAQLYGLRSERNWGIGDFTDLSNLIEIAAGLGGALVGVNPL
                     HATQGSPYSPSSRHALNFLYIDVEAVPGYEPSVKLRRRLQALRESDLVDYAAVRRVKL
                     QVLELLFRVSKPRVKVRTKGLRDYAVFETLREEFGDGWQNWPEAFHRPESQAVKAFAR
                     KNANRVAFHAWLQAIAREQLDGALRHAHELGMPLGVYVDLALGADRGGAEVWSDQDAY
                     AVGASTGAPPDEFNPRGQDWGLPPYSPRALRAIAYRPFIELLRANMPVGGALRMDHVM
                     ALSRLWWIPQGEKPDRGGYVAYPFRELLAILAAESRRARCLVIGEDLGTVPAELRTAL
                     NQAGVLSYRPMLFEKDSSGEYSSPGAYPREALVCATTHDLPTWRGYWETHDLKLRQRF
                     GLSVNFENEVSAREEEKKKLAQALKREGLGSSARSAHAFIARTPSKLAMLQPEDVFEL
                     LDQANLPGTVDQHPNWRRKLPLALERWSSHPEVSALRDVMAERRVPIPAPRATYRLQF
                     HKGFKFSDATRLVPYVARLGISHVYASPFLKARPGSTHGYDVVDHNQVNPEIGTHGEL
                     KKLIRTLKEHGMGLVVDLVPNHMGVLHADNPWWLDVLENGARSRYARFFDIDWSPGKL
                     LLPVLGKHYGEALEEVKVERKDNKWYVRYFDHRFPLNAKSTRNLGQPPEDSLALHRLL
                     EKQHYRLAYWRVASDEINYRRFFEITDLAGLRIEDPEVFEATHAFAVRLAQRGGIDGV
                     RIDHPDGLADPRQYFERLARLFPRRWIVVEKILAEHEKLPGDWPVHGETGYRFANLLT
                     GLFIDAAAEARFDRIYRRFTGERRSFDEVARESRILIMATTLYAELHMLSNWLARIAA
                     GNRNTRDYTASGLRKALVEIAARFPVYRTYVSASGVSESDRRVIDSAVSAAKRATRAA
                     DPSVFDFVRSVLLLEIAPPGKRREEMLRFAMRFQQFTAPVVAKGIEDTAFYRYTRLIA
                     LNEVGGNPSSFGVSLAEFHAAAEERARRWPATMLGSSTHDTKRSEDVRARLGVLSELV
                     SGWRMTLRRWSLMNRGGISRSDEYHFYQALVGIWPGEVSPALTDRLKAYMLKAAREAK
                     ERTSWINPDTEYERSLERFVEQSLANPVFVRDVADMVSRLAHVGRLVGLSQALLKVAS
                     PGVPDYYQGTEIWDFSLVDPDNRRPVDYGLRASLLSSLRKNTFDLLQNLADGRAKLHV
                     IRKGLEVRKQFPALFQGGKYTPLYADGGREANIVAFSLSDAAHEAVAVAPRLFARLMD
                     GDDLAPLGAKAWGEARLPLEGEYVNVLTGERHEGRAGHVGIAALLATFPAALLVRP"
     gene            complement(9413..11125)
                     /gene="treZ"
                     /locus_tag="VFR57_06095"
     CDS             complement(9413..11125)
                     /gene="treZ"
                     /locus_tag="VFR57_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEU0258973.1"
                     /translation="MSVPHRIAARHSMPFGAEVLVQGGVRFRLWAPGAKDIELHVAGR
                     LIPLQSGGDGWFEHIEAQAGPGTLYRYRVDGELFVPDPASRFQPHDVDGPSEVIAPHA
                     YKWSHHAWNGRPWNEAVIYELHVGTFTPEGTYAGAAKKIGTLKELGITAIELMPLSDF
                     AGRRNWGYDGVLPYAPDSAYGRPEDLKALVDAAHAAGLMVLLDVVYNHFGPKGNYLPR
                     YAPQFFTERHNTPWGAAIDFSNEVVRQYFVHNALYWLKEYRFDGLRFDAVHAIIDESP
                     RHILEEICASVPAGKHLVLENDANQARFVGPGRYTAQWNDDSHHAYHVLATGESDGYY
                     VAYADSPAKHLARSLAEGFAYQGEVSPFSGEPRGEPSAHLPPSCFIHFLQNHDQIGNR
                     ALGERLISLAREKPLRALAAIQLLAPSPPLLFMGEEWGCRQPFLFFCDFEGELGEAVR
                     NGRREEFRRFTSFRGEIPDPLAESTFRQCVLDWSKRDAGWLAHYKKLLQLRARAIAPR
                     RFGPGNHRMLGERAFEVKWDDLTLIANCGDQSVAVSEFPRDPPLWSSGKPGDPWSVNW
                     WLTK"
     gene            complement(11147..11828)
                     /locus_tag="VFR57_06100"
                     /pseudo
     CDS             complement(11147..11828)
                     /locus_tag="VFR57_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2012 a         3937 c         3871 g         2043 t
ORIGIN      
        1 gctataaccc cgaaggcgtc accgcctaca acatcgtccc cggcttgctc ggcaccatcc
       61 tcactatgac gatggtcctt atgaccggcc ttgccatgac ccgcgagcgc gagcgcggta
      121 ctttcgagaa tctgctttcg acgccggcgc tgccgatcga ggtgatgacc ggcaagatcg
      181 tgccttacat cctcatcggg ctgatccagg tgacgctcat catcctttgc gcgctctggc
      241 tcttcaccgt tcccatgcag gggagctatt tgctgctgta tctggtcgtg ctcgtcttca
      301 tcgccgccaa cctgacgctc ggcatcacct tctcgtccat cgcgcgcaac cagctccagg
      361 cgatgcagat gacgttcttc ttcttcctgc cctcaatcct gctctcgggg ttcatgttcc
      421 cgttccgcgg gatgccgccg tgggcgcaat ggctgggctc ggcgctaccg cttacgcact
      481 tcgtcgtcct cgtgcgcggc atcatgctca agggcagcgg gctcgccgac ctgtggccgc
      541 agatctggcc gatactcgtg ttcatgctgg tcgtcatcgc catcggattg cgcttctaca
      601 agcggacgct cgattgaagg ccgagttccg cgaccgcgac gcggcgggaa cgcgtcttgc
      661 cgcgatgctc ggaaagtaca ggggcggcag cgcgatcgtt ctcgccattc ctgcgggcgg
      721 tgagccggtc ggcgcggcga tcgcgcgcga cctcggcctg tccctacgcg ccgcgccagt
      781 gagcaaaatg ctctatccct ggacgaccga aagcggcttc ggcgcggtcg ctttcgacgg
      841 cagcgcatgg atcgaccagg cagccgtgga aggctacgga ttgacctctt cccaggtaac
      901 gaaggccacc gccgacgcgc gtgccaaggt cgagcggcga agcaagaagt tgtccagtgc
      961 gaacgccttg agagacctct ccggcaagac tgtaatcctg gtcgacgatg gcatagcggc
     1021 tgggtccacc atgaggaccg cgatcgacgc gctgcgtaag ctcggcgcgg gtcgcgttgt
     1081 tgtcgcggtg cccaccgcgc ccgcgcgctc gctagaagcg atgcgcaagc ttgccgacga
     1141 gatcgtatgc gcagacatcc gcagcggcgc gtcgtttgcc gtggcggacg cgtacgagga
     1201 atggcgtgac ctgtccgacg acgaagtgga ggcgatgctg aggccatgac ccccgtcatt
     1261 gccatccacg gcggcgcggg cgtgctacac gccgacaagc ctggtgcgca ccatcgtgcc
     1321 gtgctgaaac aggcactgga cgcgggttac gagattctga aaagggatcg atcgagcctg
     1381 gacgccgtgg ccgccgcggt agtagtgctc gaggactcac cgcttttcaa tgcggggcgg
     1441 ggctcgtcgt tcaacatgga cggcgagatc gagatggacg cctcgatcat ggacggcgcg
     1501 acgctgcgcg ccggtgccgt cgccgcggtg cggcgcatcc gcaacccgat acttgccgca
     1561 cgcgcggtga tggagaagag ccgccatgtc ttgctcgccg ggagcggcgc cgagcgcttt
     1621 gcacgcaagc aaggattgaa gttcgaacta cctgagtact ttcatacggc gcgtcggcgg
     1681 actgccttga agaagaacct gcgcaactac cacggcaccg tgggcgcggt ggcgctcgac
     1741 gttgatggaa acctcgccgc cgcgacgtcg accggcggct acacgggcaa gatgcctggg
     1801 cgcgtcggtg attcgccgct cgtcggcgcc ggaacgtacg ccgacaatcg cgcctgcgcg
     1861 gtgtcaggca ccgggctcgg cgaagcgttc atccgcgcag ccgtggctta cgacgtgtgc
     1921 gcaagaatgc gctacgccgg cgccacactc gccgcggccg cggccgacgc cctcaaggcc
     1981 gtagcggcgc tcggcgcgga cggcggggtg atcgcggtgg accgccgcgg tagtgtcgcc
     2041 atgccgttca acagcgacgg gatgttccgc gcgtgcatcg atcgccgcgg ccggcgcacg
     2101 atcgcgatat tttgaaagtc gagctgcgct atgcgccgct tccgtggatc ggcgccatcg
     2161 cctggcacta ctggttcgtg gtgtgcgacg acaccgggcg tcaccgctgg gaagtgtggc
     2221 agacgaagaa cgccggcggc tggtgcatcg gccacgtcca ccgcgacctc aagcaccccg
     2281 atgacggcgt gggcggcggc ccttccagac tcgtgaggga atgggacgga gaccacgccg
     2341 tgaagatact tcgggtacta cagacgatag aaagctatcc ctactgcgag cgttaccggt
     2401 actggcccgg gccgaacagc aatacgttcg tcgcctgggt gctgcgcgag gccggtatcg
     2461 actacaaact aagccgcaaa gggctcggcc agggttatct gaccgcgaaa cagtagaaca
     2521 gcccgtcgcc gcccgagctg cgcagcgcgt cctggctgca gccgcgcgag ggatgcgagg
     2581 agttccacga cttcgaggcg tcgtcgtcgc gcaagcctac acggtcgtga tgcccgacca
     2641 tggcggcgcc ctcgccgctc ttggtccagt tgccgcaggt caggtccttg tccgcgatgg
     2701 cgcggccgtc gggtgtcgag ccggtgagaa tgtcgtggcg gttcggactg tcgccgcgcc
     2761 cattcaccat gttgcctttc tcgtcgaggc cggtctgctt gtcgatcttc ggatccttgt
     2821 gcaggtcgtc gacgctcttg gcaacctcct cgcctttcgc gttctgccaa gggcccttgc
     2881 cgatccggtc gcgggcattg accttgtcgg tgctgaggta ggcccgccat tcccgctggc
     2941 cggcccccgc tgtctgcgcg agcttctggc aatgggcatc cgcgcccgcg aggccgccaa
     3001 ggtctgcccc tttgcccggc ccgacgctgg tgatgaagaa gctcatgtcc ttttgctgcg
     3061 cctgcgcgct gagcgctacg agcaggccga tcgtcgtaag aagagttttc atgctgctgt
     3121 aaacagggtc ctcgcccctt tatttcaagt agctgtcctg gagagtgcgt cggacaaagg
     3181 cgcgcgcgtc gtctcgatcg aacggcgcgc cttcgaccag ctggtcggcg taccggaaga
     3241 acggcaggaa gagctgcgcc tggtccaccg gcgcgcgtac ggtgaagcgg ccgcgcccct
     3301 cctcgagctc gcgccaggcg gcgcgcacct ctgtccagaa cggctcggtg cgcgcgtaat
     3361 acttttcacc ggcgccaaag tcgtagtccc ttatgcgctg gtatcgggcc acgccgtatt
     3421 cgcgcgcaag gtacttcctg tccttcagcg ccagcttgag gttgttttct tcctgcaccc
     3481 agcccgtcgg cgtgatggtg tggcggttgg tcccgacaag cacgtcgtaa tccttgcgca
     3541 cgctgaactc gcgccgcggg agcggccgcc aggtctcgtc gctgatccag gtcgatacgc
     3601 cgtccgagtg ctcccagcgc ccgagcgcgg cgtagcgcgg cgagtcgtcg acctggagca
     3661 ccgtctgtga ccaggcaccg cggcgcgcgg cggcgggcgt ggcgcggcgc gcccaggtgt
     3721 tctccccctc gtacgcgagg agcgtcggcg cctcgtagcg ccactcctga cgccaatggc
     3781 gcaccaccat gggctcgctc acgatgggag aaccgccact gcccccgtct cgcagcatgc
     3841 gcatcaccag gatgtgctgg agcgcgatga actccggccg gtcctcggtg acgaacacgt
     3901 actcggtacc ccagctctgg taaggggcgt cctgcttgag cgagggctcg tagcgcacta
     3961 cttcgaggaa atcgaagctc acccggtagg gcccggccat cgccaggatc gcgcgccgat
     4021 cgcgctcttg agcgcccagg ccgggctcgt tcagccgttt ccaggattct gtcggcgccg
     4081 cgtcgagcgt caccggcgca ccgcgcgtcg tcgcgccgcg cggcttgagc gccttctcgt
     4141 cgagcggcca ggcgaagatg aactggctcg gctcagctgc cctggctgct ccgcaagcaa
     4201 gcgcaaaaag aactatccag aacgttttca tgcctgtccc catacctcca tcgcggtctc
     4261 caccacccgt cgcagcttcg cctcctgatc gcggaatgac agcatcgtgc ctttctcgtg
     4321 gctggcgaag ccgcattgcg ggctgatcga gaggctatcg agcggaatga ggcgcgccgc
     4381 ggcgtcgatc ttctctttca atgcatcctt gtcttccagc gccggggtct tcgtggaaac
     4441 caggccgagc actacgcgct tgccgttcgg aacgaagcgc agcggctcga agccgccggc
     4501 gcgctcgctg tcgaactcga gaaagaaagc atcgacctcc atgcgggaaa aggacttttc
     4561 cgctatgcgc tcgtagccgc cctcggcgat ccacttaccc atggcgttgc cgcggcacat
     4621 gtgcacgccc accgtcatgc cggccggccg ctcgcgcacc acgcggttga tgagatctat
     4681 atacaggtcg ataagcttct ccgggtcgtc gccgcgcgcg cgcaccgcgt cgcgcagctt
     4741 ctggtcgcaa agcagggtct gcgccacttc gtcgatctgc aggtagcgcc cgccggcggc
     4801 gtgcagcgcg tgcagctcct cgacatacac cttcaccacg tcggcgaaga actcatcgag
     4861 gtcgggataa acgtcgcggt cgacgcaatc gcgcccgcgc gggtagtgca gataggacgg
     4921 agccggcagc gtcgccttaa cgacccggtg cgtgtgcttc agcgagaacc cgacctcgtc
     4981 ggccacgatc ggtccggtgc gccggatccg tgtcaccgcg accggcgccg atccgacgcg
     5041 catcacgttg ccggccgcgt cgcgctgcag gtcgacgttg cccaccgcgt cggcgcgcgc
     5101 gaagccagcg accttcgaca cgaagccgag ccgccaggac tcgcggcgaa actcgccgtc
     5161 ggtcacgacc ttcaggccga ggctctcctg ccaccttatc gcttcgacga tacactcgtc
     5221 tgcgcacgag cggtcctgca gaagccgctg cggccgcagc aggctgccga tatgctcggc
     5281 gcggaagggc ggcgcgctca cggccgcacg agcagcgcgg ctggaaaggt ggcaagaagc
     5341 gctgctatgc ccacgtgacc tgcgcgaccc tcatgccgtt cgccggtcag tacgttgacg
     5401 tattcgcctt cgagaggcaa gcgcgcctcg ccccaggcct tcgcgccgag cggcgccagg
     5461 tcgtcgccgt ccatcaggcg cgcgaagagg cgcggcgcca ctgccaccgc ctcgtgcgcg
     5521 gcgtcggaaa gcgaaaacgc gacgatgttc gcctcgcggc cgccatcggc ataaagcggc
     5581 gtgtatttcc cgccctggaa cagggccgga aattgtttcc tcacctccag gcccttcctg
     5641 attacatgca gcttcgctcg gccgtcagca agattttgca gaaggtcgaa cgtattcttt
     5701 cgtaaggatg agagcaaact tgcgcgcaac ccgtagtcga ccggccggcg gttgtcgggg
     5761 tcgacaaggc tgaaatccca gatctcggtt ccctggtaat aatcgggcac ccccggcgag
     5821 gcgactttga ggagcgcctg cgaaaggccg acaaggcgcc cgacgtgcgc gaggcgcgac
     5881 accatgtcgg ccacgtctct tacgaaaacc ggattcgcca gcgactgttc gacaaagcgc
     5941 tcgagcgagc gctcgtattc cgtgtccgga ttgatccagc tggtccgctc cttcgcctcg
     6001 cgcgccgctt tcagcatgta ggccttcagc cggtcggtca gggcgggcga gacctcgccc
     6061 ggccagatgc cgacgagcgc ctggtaaaaa tggtattcat cgctgcgcga gatgccgccg
     6121 cggttcatga ggctccatcg ccgcagcgtc atgcgccagc cggagacgag ctcggacagc
     6181 acgccgaggc gcgcgcgcac atcctcggag cgcttggtgt cgtgcgtcga gctccccagc
     6241 atcgtcgccg gccagcggcg tgcccgctcc tccgccgctg catggaactc cgcaagcgag
     6301 acgccgaagc tcgagggatt gccgccgacc tcgttcagcg cgatgaggcg cgtgtagcgg
     6361 tagaacgcgg tgtcctcgat ccctttcgcc accacgggcg ccgtgaactg ctggaagcgc
     6421 atcgcgaagc gcagcatttc ttctcggcgc ttcccagggg gtgcgatttc gaggagcagg
     6481 acgctgcgca cgaaatcgaa cacgctcggg tcggccgcgc gggtggcgcg tttggccgcg
     6541 cttaccgccg agtcgatcac ccgacggtcg ctttccgata cgccgctggc cgagacgtag
     6601 gtgcgataga ccgggaaccg agcggcaatc tcgacgagcg ccttgcgcag cccgctcgcg
     6661 gtgtagtcgc gcgtgttgcg attgccggcg gcgatgcgcg caagccaatt ggacagcatg
     6721 tggagctcgg catagagcgt cgtcgccatg attaggatgc ggctctcgcg cgcaacctcg
     6781 tcgaagctgc gccgctcgcc ggtgaagcgc cgatagatcc ggtcgaagcg tgcttcggcg
     6841 gccgcgtcga tgaacaaccc cgtcagcaga ttggcgaagc ggtaaccggt ctcgccgtgc
     6901 accggccagt cgccgggcag cttctcgtgc tcggcgagga tcttttccac cacgatccag
     6961 cggcgcggga aaagccgcgc cagccgctcg aagtactgcc tcggatcggc gaggccgtcg
     7021 ggatggtcga tgcgcacgcc gtcgataccg ccgcgctgcg cgagccgcac ggcaaacgcg
     7081 tgcgttgcct caaacacctc ggggtcctcg atcctcaggc cggcgaggtc ggtgatctcg
     7141 aagaagcgcc ggtagttgat ctcgtcggag gcgacccgcc agtacgcgag ccggtagtgc
     7201 tgcttctcga gcagccggtg cagcgccaag ctatcttccg gtggctgccc gagattgcgt
     7261 gttgactttg cattcagcgg aaagcggtgg tcgaagtagc ggacgtacca cttgttgtcc
     7321 ttgcgctcga ctttcacctc ctcgagcgcc tcgccgtaat gcttgcccag gacgggaagc
     7381 agcagcttgc caggcgacca gtcgatatcg aaaaaccgcg cgtaacgcga tcgagctcca
     7441 ttctccagca cgtcgagcca ccatggattg tcggcgtgca gcacgcccat gtggttcggc
     7501 acgaggtcca cgaccaggcc catgccgtgc tctttcaacg tgcggatgag cttcttcaac
     7561 tcaccgtggg tgccaatctc gggattgacc tggttgtggt ccacgacgtc gtagccgtgc
     7621 gtactcccgg ggcgcgcttt caggaacggc gaggcataga cgtggctgat gccaagcctc
     7681 gcgacataag gcacgaggcg agtagcgtcg ctgaacttga agcccttgtg aaattgcagt
     7741 cggtacgtag cacgcggcgc agggatggga acccgcctct ccgccatgac gtcgcgaagc
     7801 gccgagactt ccgggtgtga gctccagcgc tcgagcgcga gcggcagctt acggcgccag
     7861 ttcgggtgtt gatcgaccgt gcccgggaga ttcgcttgat cgaggagctc gaacacgtcc
     7921 tcgggttgca gcattgcgag cttcgacggg gtgcgggcga tgaacgcatg cgccgatcgc
     7981 gccgaggagc ctaatccttc ccgcttcaag gcctgcgcga gctttttctt ttcctcttcg
     8041 cgggcagaga cttcgttttc gaaattgacg ctcaagccga aacgctgccg aagcttcaga
     8101 tcatgcgttt cccagtagcc gcgccaggtc ggcaggtcgt gcgtggtcgc gcacaccagc
     8161 gcctcgcggg gataagcgcc gggggaactg tactcgccgg acgagtcctt ctcgaaaagc
     8221 atcggccgat acgagagcac gcccgcctgg ttaagcgcgg tgcgcagctc ggccgggacc
     8281 gtgccgaggt cctcgccgat caccaggcat cgcgcgcgcc ggctttccgc ggcgaggatc
     8341 gcgagcagct cgcggaacgg gtacgcgaca tagccgccgc ggtcgggttt ctcgccctgc
     8401 ggtatccacc acagccgcga cagcgccatg acgtggtcca tgcgcagcgc accgccgacc
     8461 ggcatgttgg cgcgaagcag ctcgatgaac ggccggtacg ctatcgcgcg cagcgcgcgc
     8521 ggcgaatacg gcggcaaacc ccaatcctgg ccgcgcgggt tgaactcgtc gggcggcgcg
     8581 ccggtggagg cacccacagc gtaagcatcc tggtcggacc acacctccgc cccgccgcga
     8641 tcggcgccga gcgcgaggtc gacatacacc ccgagcggca taccgagctc gtgcgcatgg
     8701 cgcagcgcgc cgtcgagctg ctcccgggcg atggcctgca accaggcatg gaaggcaacg
     8761 cggttcgcat ttttcctggc aaacgccttc accgcctgcg attcaggacg atggaatgct
     8821 tccggccagt tctgccagcc gtcgccgaac tcctcgcgca gggtctcgaa caccgcgtag
     8881 tcgcgcaggc ccttcgtacg caccttcacc cggggtttac tgaccctgaa taggagctcg
     8941 agaacctgga gcttgacgcg gcgcaccgcc gcgtaatcga caaggtcgga ttcgcgcagt
     9001 gcctgcaagc ggcggcgaag ctttaccgag ggctcgtagc cgggcacggc ttcaacgtcg
     9061 atgtagagga agttgagcgc gtggcggctc gagggactgt aggggctgcc ttgcgtggcg
     9121 tggagcgggt tcacgccgac caacgcgccg ccgagcccgg ccgctatttc gatcagattg
     9181 ctgaggtcgg tgaaatcgcc gatgccccag ttgcgctctg agcgcaggcc gtagagctgc
     9241 gccatgaagc cccagagcct gcgctcttcg agcagcttcg gctggtagca gcgcccggtc
     9301 gcgaggcgca ccttgcgcgc cctgcgcctg ggccccatcg cctcgagcag cacgcgccgc
     9361 acctcaggct cgatcgactt ctcgttgccc cagacgtcga gatagctcga agctacttcg
     9421 tcagccacca gttcaccgac cacggatccc caggcttgcc gctcgaccag aggggcggat
     9481 ccctgggaaa ctcgctgacc gcgaccgact gatcgccgca gttcgcgatc agcgtgaggt
     9541 cgtcccattt cacctcgaat gcgcgctcgc ccagcatgcg gtggttgccc gggccgaacc
     9601 tgcgcggggc gatcgcacgc gcgcgcagct ggagcagctt cttgtaatgc gcaagccagc
     9661 ccgcgtcgcg cttgctccag tccagcacgc actgcctgaa agtgctctcg gcgagcggat
     9721 cgggaatttc gcccctgaag ctcgtgaagc gcctgaactc ctcccgccgc ccattgcgca
     9781 ccgcctcgcc gagctcgcct tcgaaatcgc aaaagaagag gaagggctgc cggcaacccc
     9841 actcctcgcc catgaacagg agcggcggcg acggggcaag gagctggatc gcggccagtg
     9901 ctctgagggg cttttccctc gcaagagaga tcaacctttc ccctagcgcg cgattgccga
     9961 tctggtcgtg gttctggagg aagtggataa agcacgaagg cggcaggtgc gcgctcggct
    10021 cgccgcgcgg ctccccgctg aagggcgaga cctcgccctg gtaggcaaag ccttcggcaa
    10081 gcgagcgcgc aaggtgcttc gccggggaat cagcgtaggc gacgtagtag ccgtcggatt
    10141 cgccggtggc gagcacgtgg tacgcgtggt gcgagtcgtc gttccactgc gcggtgtagc
    10201 gcccggggcc gacgaagcgc gcctggttgg cgtcattctc gagcacgagg tgcttgcccg
    10261 cgggaaccga ggcgcagatc tcctccagaa tgtggcgcgg cgactcgtcg atgatggcat
    10321 gcaccgcgtc gaagcgaagt ccgtcgaagc ggtactcctt gagccagtag agcgcgttat
    10381 gcacgaagta ctggcgcacc acttcgttcg agaaatcgat cgccgctccc cacggcgtat
    10441 tgtgccgttc ggtgaagaat tgcggcgcgt agcgcggcag gtaattcccc ttagggccga
    10501 aatggttgta gaccacgtcg agcaggacca tgaggccggc ggcgtgagcg gcgtccacga
    10561 gcgccttcag gtcctcgggc cgaccgtatg cggaatcggg cgcgtacggc agcacgccgt
    10621 cgtagcccca gttgcgcctg cccgcgaaat cggaaagcgg cataagctcg atcgccgtga
    10681 ttccgagctc cttcaacgtg ccgatcttct tcgctgcgcc ggcataggtg ccttccggag
    10741 taaacgtgcc gacgtggagc tcgtagatca ccgcttcgtt ccagggcctg ccgttccatg
    10801 cgtgatggct ccacttgtag gcatgcggcg cgatgacctc gctcggcccg tcgacatcgt
    10861 gcggctggaa acgcgacgcc gggtcgggga cgaagagctc gccgtcaacg cggtagcggt
    10921 agagcgtgcc cgggcctgcc tgggcttcga tatgctcgaa ccagccgtcg ccgcccgatt
    10981 gcagcgggat caggcggccg gcgacatgta attcaatgtc tttggccccg ggggcccaga
    11041 ggcgaaagcg tacgccgccc tgcaccaaaa cctcggcacc gaacggcatg gaatggcggg
    11101 cggcaatgcg atgtggaacg ctcacggtag attgatccga cccctttcac ggttcagggt
    11161 ttcctgcggc gcggccacca gcgctcgagc atctcggcgg cccaatcttt gccgccgagc
    11221 ccgaacgcga gcgcaagcgc gaacaccacg ccggcgagca cgataaggaa ggtctggcgc
    11281 acgatgtcgc cgccgacgtt cacctgatcg agcgcgatca gcaccacgaa cgcgaggatc
    11341 gcgtactgcg agatgcggcc gaggagctcc gcgtcctgca gctggacctt cctgcagtat
    11401 gcggtcacgg cgtggccgat aaagccggcg aagtacgcgc cgaaagcgag aatcagcatc
    11461 gccaccatga ccttcggcac gaagagcagc acccggctca agaggtcggt gatgtaggcg
    11521 agaccgaggc tgttgaagcc gatcaccagc gcgaccagga tgacgaccca gtagaagagc
    11581 agcgccacga tctcggtgcc gtcggcgcgg atgccgccgc cgcgcaggaa gccgtcgatg
    11641 ccggcgcgct cggtcagcac gttgaagttc acggcgcgca gaccccgcac gatcgcgaac
    11701 ttcaccgcct tggcgatgaa gtatccgatg atgacgacca gcagcgccag ggccagccgc
    11761 ggcaggaaat cgcccatctg gcgcagaaac acgcgcaggg gctcgagcag cacctcgaag
    11821 cggtccatat ctacatcctc ccgttcagat caccctgtcg gcg
//
LOCUS       DASTPD010000182         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_92389, whole genome shotgun sequence.
ACCESSION   DASTPD010000182 DASTPD010000000
VERSION     DASTPD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92389"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFR57_06105"
     CDS             complement(<1..438)
                     /locus_tag="VFR57_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0258974.1"
                     /translation="MLKLSRPAAEVFIPDGTSWEAAAARVTHLVVAAHADDVEIMAWH
                     AVLHAAGLAAVIVTDGRGSDTRLREQKRAASLGNYAVLIWLDHASADVKQAAYPALAS
                     DLSAVLSAVRPRLVYTHNPADKHDTHVAVALHTVRALRANPSSV"
     gene            complement(644..1477)
                     /locus_tag="VFR57_06110"
     CDS             complement(644..1477)
                     /locus_tag="VFR57_06110"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0258975.1"
                     /translation="MSGSTLVSTGELAANLAKWRVFDCRHDLANPKLGEQQYREGHIP
                     GALFAHLDRDLSGRKTGANGRHPLPDPQDFEKWLEKTGLTPQEQAVCYDGGPGAMAAR
                     LWWMLRWVGHERVAVLDGGLAKWTKEGRPVTRDVPRFTPFNYPIRLRHDVAADVGAVE
                     RSLGKSLLLDARAPARYRGEQEPIDPVAGRIPGAKNRFNMDNLSPDGTFKTPAQLKRE
                     LEPLLRDDSPAGVIHYCGSGVAACHNLLAMEIAGLEGGKLYAGSWSEWIADSARPRER
                     G"
     gene            complement(1446..2543)
                     /locus_tag="VFR57_06115"
     CDS             complement(1446..2543)
                     /locus_tag="VFR57_06115"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0258976.1"
                     /translation="MADVPQPGKLTPASCQLPVAWYFDPRIFEAEKRLLFQAGANYVG
                     HELMVPNAGDWHTLAWMDHAKLLVRNAGGVELLSNVCRHRQAIMLQGRGNAENIVCPV
                     HRWTYDMKGELLGAPRFADQPCAKLAATPLKRWNGLLFAGPRDPATDLAKLTTVADWD
                     FSGFMLDSVRIDDYDVNWKTFIETYQEVYHVDLVHPGLGNFANCDGFTADYGADYSVQ
                     IVPAKAGLARPGTPSYKRWHEVCLKHLDGRAPKYGALWMTYYPGLMLEWYPNVLVISH
                     LIPRSPTRTTNVVEFYYPEEIALFEREFIAAQQAAYVETAVEDNEICMRLDRGRRALY
                     EQGLDDAGPYQSPMEDPEVHFHEWLHARLDR"
     gene            2584..2835
                     /locus_tag="VFR57_06120"
     CDS             2584..2835
                     /locus_tag="VFR57_06120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0258977.1"
                     /translation="MSKPSGGLDELDFEKALAELEKIVARMESGELSLEQALATHKRG
                     LELARFCQQKLEAAQQQVKVLEGEVLKPLAAAAEPDEDS"
     gene            2876..>3691
                     /locus_tag="VFR57_06125"
     CDS             2876..>3691
                     /locus_tag="VFR57_06125"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU0258978.1"
                     /translation="METVLARVLPAAAIAPARLHEAMRYATLEGGKRVRPLLAFAAGE
                     VSGASPERLEVAAAAVELVHAYSLVHDDLPCMDNDVLRRGKPTVHVEYDEATALLVGD
                     TLQSLAFQLLAEHRLADDPHRQLEMVKTLALAAGSRGMGGGQQIDLEATGKTLDLPEL
                     EFMHIRKTGALIRAAVLLGVACGSNAEPQKQKLDKYAKAIGLAFQVVDDVLDYDASTA
                     TLGKTAGKDSKQGKPTYVSAMGVAGARRLAEELRAEAHAALAGIGAPARRLGEL"
BASE COUNT          630 a         1177 c         1241 g          643 t
ORIGIN      
        1 caccgacgac ggattcgcgc gcagcgcccg cacggtgtgc agggctaccg cgacgtgcgt
       61 gtcatgcttg tccgccggat tgtgggtgta gacgagccgc ggccgaaccg cggacagcac
      121 cgcgctcaag tcggacgcga gcgcaggata ggcggcctgc ttcacatccg cgcttgcgtg
      181 gtccaaccag atcagcaccg cgtagttgcc gagcgacgct gcgcgcttct gctcccggag
      241 ccgtgtgtcg ctacctcgtc catcggtgac gatgacggcg gcgagtcccg ccgcatgcag
      301 aaccgcatgc caagccatga tctccacgtc gtcggcatga gctgccacta cgaggtgagt
      361 cacccgcgcg gcagccgctt cccaggatgt accgtcggga atgaagacct ccgcggcggg
      421 gcggctcagc ttcagcatgc aacgagttta gtgcctctgc gtccgtgaat cgacggcgag
      481 gcggttggat gactgcttct ggccagtcag cgcccttccg acgaaggtcg cgtttgcgat
      541 gccagagcgg ggacactcag ggcacgcggt ggtccagcgt tcgtctgcaa ccgttgcgcg
      601 agagcgaacg ctgcgagtta tggtttcgag gccgtcgccg tcgctacccg cgctccctgg
      661 gccgcgcgga atccgcgatc cactcgctcc aggagcccgc atagagcttg ccaccttcca
      721 ggccagcgat ctccattgcg aggaggttat ggcatgcggc gacgccggag ccgcagtagt
      781 ggatgactcc ggcgggcgaa tcgtcgcgca gaagcggctc gagctcccgc ttgagttgcg
      841 cgggggtctt gaacgttccg tcgggcgaga ggttgtccat gttgaagcgg tttttcgccc
      901 cgggaattcg gcccgcgacc gggtcgatcg gctcctgctc gccgcggtag cgcgcgggcg
      961 cgcgggcatc gagcaacaag gacttcccaa gagatctttc gactgctcct acgtccgcgg
     1021 ccacatcgtg ccgtagccgg atcgggtagt tgaaaggagt gaaacgcggc acgtctctag
     1081 tcaccggacg gccttccttc gtccactttg caagcccgcc gtcgagcacg gcaaccctct
     1141 cgtgcccgac ccagcgcagc atccaccaca ggcgcgccgc catcgcgcct ggtccgccgt
     1201 cgtagcagac ggcctgctcc tgtggcgtca atccggtttt ttccagccac ttctcgaaat
     1261 cctgcgggtc gggaagggga tgccggccgt tcgcgccggt cttgcggccg gagaggtcgc
     1321 ggtcgagatg cgcgaacagc gctcccggaa tatggccttc gcggtattgc tgctcgccga
     1381 gcttgggatt ggcgaggtcg tggcggcagt cgaagacgcg ccacttggcg aggttcgccg
     1441 ctagctcacc ggtcgagacg agcgtggagc cactcatgga agtgcacctc cgggtcctcc
     1501 atcggggact ggtagggacc ggcgtcgtcg aggccttgct cgtagagggc gcgccgcccg
     1561 cggtcaaggc gcatgcagat ctcgttgtcc tcgaccgcgg tctcgacata cgcggcctgc
     1621 tgcgccgcga tgaattcgcg ctcgaagagc gcgatctcct cgggatagta gaactcgacc
     1681 acgttagtgg tgcgcgtcgg cgagcgcggg atgaggtgag agatcacgag cacgttcggg
     1741 taccactcga gcattaggcc cgggtaatag gtcatccata gtgcgccgta cttcggcgcc
     1801 cggccgtcga gatgcttgag gcagacctcg tgccagcgct tgtaactcgg cgttccggga
     1861 cgcgcgagcc ccgctttggc tggcacgatc tggaccgaat agtcggcgcc gtaatcggca
     1921 gtgaagccgt cgcagttggc gaaattgccc agcccgggat ggacgagatc aacatggtag
     1981 acctcctggt aagtctcgat gaaggtcttc cagttgacgt cgtagtcgtc gatgcgcacc
     2041 gagtcgagca tgaagccgct gaagtcccag tccgcgaccg tagtgagctt cgcaagatcc
     2101 gtcgccgggt cgcggggtcc cgcgaacagc agcccgttcc agcgcttgag cggcgtggcg
     2161 gcgagcttcg cgcagggctg gtcggcgaac cgcggcgcgc caagcagctc gcctttcata
     2221 tcgtaggtcc agcgatgcac cgggcacacg atgttctcgg cgttcccgcg cccttgcagc
     2281 atgatggcct ggcggtggcg gcagacgttc gagaggagct cgacgccacc cgcgtttcgc
     2341 acgagcagct tggcgtggtc catccacgcg agcgtgtgcc agtcgcctgc gttaggcacc
     2401 atcagctcat ggccgacata gttcgctccg gcctggaaca gaagccgctt ttccgcttcg
     2461 aagatgcgcg ggtcgaagta ccaggctacc ggcagctgac agctcgccgg tgtcagtttt
     2521 ccaggttgcg gaacatccgc catgcgttgt aaagctccta gaaaaacagt attttagcaa
     2581 acgatgagca agccttcagg cgggctcgac gagctcgact tcgaaaaggc gctcgccgag
     2641 ctcgaaaaaa tcgtcgcccg catggaaagc ggcgagcttt cgctcgagca ggcgctcgcc
     2701 acgcacaagc gtgggctgga gctggcgcgc ttttgccagc agaagctcga ggctgcccag
     2761 caacaggtca aggttctcga aggagaggtt ctcaagccgc tcgcggctgc ggccgagccc
     2821 gatgaagatt cttgatagcg agtttcaggc ctggatgggc ggcgtccagg cgcgcatgga
     2881 aacggtgctc gcgcgcgttc ttccggcggc ggcgattgcg ccggcgcggc tgcacgaagc
     2941 catgcgctat gcgacgcttg aaggcggcaa gagggtacgg ccgctgctcg cctttgctgc
     3001 gggcgaagtg tccggagcct ccccggagcg gctcgaggtg gctgcggcgg cggtggagct
     3061 agtccatgcc tattcgctcg tgcacgacga cttgccctgc atggacaatg acgtcctgcg
     3121 tcgtggtaag ccgaccgtgc acgtcgaata cgacgaagcc accgcgctcc ttgttggcga
     3181 cacgctgcag tcgctcgctt tccagctcct ggccgagcat cgcctggccg acgatccgca
     3241 ccgccagctc gaaatggtga agacactggc gctcgccgcc ggctcgcgcg gcatgggggg
     3301 cggccagcag atcgacctcg aggccaccgg caagacgctc gatcttcccg agctcgagtt
     3361 catgcatatt cgcaagaccg gggcgctcat cagagccgcg gtgctgctcg gcgttgcctg
     3421 cggaagcaac gcggagccgc aaaagcagaa gctggacaaa tacgccaagg cgatcggcct
     3481 cgctttccag gtcgtcgatg acgtgctcga ctatgatgcg agcacggcaa cgctcggcaa
     3541 gacggcggga aaggactcga agcagggcaa gcccacctac gtctctgcga tgggagttgc
     3601 cggcgcgcgg cgcctcgcag aggaactgcg cgcagaggcg catgccgcgc tcgcgggcat
     3661 cggcgccccg gcgcgccgcc tcggcgagct c
//
LOCUS       DASTPD010000183         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_502277, whole genome shotgun sequence.
ACCESSION   DASTPD010000183 DASTPD010000000
VERSION     DASTPD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502277"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFR57_06130"
     CDS             complement(<1..502)
                     /locus_tag="VFR57_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0258979.1"
                     /translation="MKRFVPLFLLLLSMPVAGQGVLPVDRIVAVVNKDVITATELSEA
                     VAAAQRQLRRQKTPLPERGLLERQMLERLILDKAQLQFARDKGIRIDELQLDRAVQRV
                     VQNNNMSLADFRRALERDGVSFQAWREDLREQMLLTRVREREVDDRIQVSDTEIDLFL
                     AELQAKP"
     gene            complement(507..2723)
                     /locus_tag="VFR57_06135"
     CDS             complement(507..2723)
                     /locus_tag="VFR57_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEU0258980.1"
                     /translation="MAMRFAMLAVALAAAAPAAAQTAKPPAEADRTTIEADVIEGVSD
                     LEVGARGNAEIRRDDMSIFGESLHYNREFGRAEGEGGVRLQRGADRFFGPRLHYNTLD
                     DTGVFDSPTYLLERDRTARGSAERLEFLGPDRYRFINANYTTCRPGQEDWRLEASELE
                     LDFEEEDGAAVHPRLRFFDTTILAAPYAVFPIGERRKSGLLTPYYAQTSQRGFEFGIP
                     YYWNIAPEYDATFTPVVMGKRGYQLKSEARYLGRPYLGELKFEYLPDDRQTGTSREGV
                     SWQHRHNFPRNVVAQVDYNRVSDDSYFVDLASTVKQVSVSNLPQDAHLTHSGSFGRAP
                     YSVQARVQRFQTLQDPLAPIVPPYHRIPQLNFSAAYNDLAGAFDAALPLEYVQFDHPT
                     LVEGSRASTTPTLALPLLAPGWFFTPKIGLRYASYSLSLDEPARTPELSVPWGSLDSG
                     LVFERDSVFFGPGSTQTLEPRLFYAYIPFRNQDAIPLFDTALADFTYAQLFTENRFVG
                     GDRFGDANQLTLALTTRLLYADGQEGLRATVGQRWYFEDERVGLTPTSPLRTANESDI
                     LASVGGRFGGHWTFDVTTQYNRQLQRVERVSTGVSYRPESAKVLNASYRFQREVLEQV
                     DISGQWPLRPGWYGVGRYNYSIRDDKLLEGLAGIEYNAGCWVFRGVVARIQASTQVSS
                     TAFIFQIEFNGIGALGTDEVVGILRRNVPGYSVTNPAESRLAPADARPRLPFDQVY"
     gene            2762..>3206
                     /locus_tag="VFR57_06140"
     CDS             2762..>3206
                     /locus_tag="VFR57_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0258981.1"
                     /translation="MIHGDLRRAALERWLAPQFGVSFQLTPASEDASFRRYLRATLAD
                     GRTYVAMDAPPQKEDCRPFVRVAHMLSAAGVHAPDIIAQELEQGFLLLSDLGSRTYLE
                     ALNDANSAALFADATEALLRWQLATRAGELPPYDEALLRREMNLFP"
BASE COUNT          529 a         1032 c         1091 g          554 t
ORIGIN      
        1 cgggctttgc ctgcagctcg gcaagaaaga ggtcgatctc cgtgtcgctt acctggatgc
       61 ggtcgtcaac ctcgcgctcg cgtactcgcg tcagcagcat ctgctcacgc aagtcctcgc
      121 gccacgcctg gaacgagacg ccgtcgcgct ccagcgcgcg gcgaaaatcc gcgaggctca
      181 tgttgttgtt ctgcacgacg cgctgcaccg cgcggtcgag ctgcagctcg tcgatacgga
      241 tgcccttgtc gcgcgcgaac tgcagctggg ccttgtcgag gatcagccgc tcgagcatct
      301 gacgctcgag caggccgcgc tcgggcaggg gcgttttctg gcggcgcagt tggcgctgcg
      361 ccgccgccac tgcctcggag agctcggtgg cggtgatcac atccttgttc accaccgcca
      421 cgatgcgatc gaccggaagg acgccttgcc ctgcgaccgg catggaaagg agcaggagaa
      481 agagggggac aaagcgcttc atactctcaa tacacctggt cgaaggggag ccgcggacga
      541 gcgtcggccg gggcgagccg cgattcggcc ggattggtta ccgagtaccc aggcacgttg
      601 cgcctcagta tcccgaccac ctcgtcggta ccaagggcgc cgatgccgtt gaactcgatt
      661 tggaagatga atgctgtgga gcttacctgg gtcgacgcct ggatccgcgc gacgacaccg
      721 cggaacaccc agcaccccgc gttgtactcg atgcctgcca ggccctccag caacttgtcg
      781 tcccggatgg agtaattgta gcgcccgacg ccgtaccagc ccggcctgag cggccattgc
      841 ccggagatgt cgacctgctc cagcacttcg cgctggaagc ggtagcttgc attcagaacc
      901 ttcgcggact cggggcggta gctgacccct gtgctcacgc gctcgacgcg ctgcagttgc
      961 cggttgtact gcgttgtcac gtcgaaggtc cagtgcccgc cgaaccgccc gccgaccgac
     1021 gcaaggatgt ccgattcatt cgccgtgcgc agcggcgagg tcggcgtcag gccgacgcgc
     1081 tcatcttcga agtaccagcg ctgcccgacg gtagctcgca gcccttcctg accgtccgcg
     1141 tacagcaagc gcgtcgtgag cgcgagcgtc agctggttgg cgtcgccgaa gcggtcgccg
     1201 ccgacgaagc ggttctccgt gaagagctgg gcgtaggtga agtcggcgag cgcggtgtcg
     1261 aaaagcggta tcgcgtcctg attacggaac gggatgtacg cgtagaagag ccgcggctcc
     1321 agcgtttgcg tcgagccggg gccgaagaac acggaatcgc gctcaaacac gaggccgctg
     1381 tcgaggctgc cccacggcac cgaaagctcc ggcgtgcggg ccggttcgtc gagcgagagg
     1441 ctgtaggacg cgtagcgcag ccctatcttg ggcgtgaaaa accagcctgg agcgagaagc
     1501 ggcagcgcga gcgtcggggt cgtcgaggcg cgggagccct ccacgagcgt gggatggtcg
     1561 aactgcacgt actcgagcgg cagcgccgcg tcgaacgcgc cggcaagatc attgtacgca
     1621 gcgctgaaat tgagctgagg aatgcggtgg tacggcggaa cgatgggcgc aagcgggtcc
     1681 tgcagcgtct ggaagcgctg aacgcgcgcc tgcacggaat agggtgcgcg gccgaacgag
     1741 ccgctgtggg taaggtgcgc gtcctgcggc aggttgctca ccgagacctg cttcaccgtg
     1801 ctcgccaggt ccacgaagta ggagtcgtcc gagacccggt tgtagtccac ctgcgcgacc
     1861 acgttgcgcg ggaaattgtg ccggtgctgc cacgatacgc cttcgcgcga ggtgccggtc
     1921 tgacggtcgt ccggcaagta ctcgaacttg agctctccga ggtaggggcg tccgagatag
     1981 cgcgcctcgc tcttcagctg atagccgcgc tttcccatga cgaccggagt gaacgtggcg
     2041 tcgtactcgg gggcgatgtt ccagtagtac ggaattccga actcgaagcc gcgctggctg
     2101 gtctgcgcat agtacggcgt cagcaagccg ctcttgcgcc gctcaccgat cgggaacacc
     2161 gcgtacggag cagcgaggat tgtcgtgtcg aaaaagcgca gccgcgggtg gaccgccgcg
     2221 ccgtcttcct cctcgaaatc gagctcgagt tcgctcgcct cgagcctcca gtcctcctgg
     2281 ccgggacggc acgtggtgta gttggcattg atgaagcggt agcggtccgg gccgagaaac
     2341 tccaggcgct ctgcgctacc gcgcgcggtg cggtcgcgct cgaggaggta ggttgggcta
     2401 tcgaacaccc cggtgtcgtc gagcgtgttg tagtgcaggc gcgggccgaa aaagcggtcg
     2461 gcgccgcgct gcaagcgcac cccgccctcg ccttcggcgc gcccgaactc ccggttgtag
     2521 tgcagactct cgccgaagat actcatgtcg tcacgccgga tctcggcgtt gccgcgcgcc
     2581 cccacctcca ggtcgctcac gccttcgatg acgtcggctt cgatggtggt acggtcggcc
     2641 tcggctggag gctttgcggt ttgcgcggct gccggcgcgg cggcggcgag cgcaaccgcg
     2701 agcatcgcga agcgcatcgc catggcgccg gagagatttt tccttggtgt tagaattttc
     2761 aatgatacac ggcgatttgc ggcgcgccgc cctcgagcgc tggctcgccc cgcagttcgg
     2821 cgtctctttt cagctgaccc cggcgtcgga ggatgcaagc tttcggcgct acttgcgggc
     2881 gacgcttgcg gatggcagga cctacgtggc gatggacgcg ccgccccaga aggaggattg
     2941 ccgacccttc gtgcgcgtgg cgcacatgct ctccgcagcc ggcgtgcacg cgccggacat
     3001 catcgcgcag gagctcgagc agggatttct cctgctctcc gaccttggct cgcgcacgta
     3061 ccttgaagcg ctcaacgacg cgaactcggc ggcgcttttc gcggacgcca ccgaggcact
     3121 gctgcgctgg cagctcgcga cgcgcgccgg cgagctgccg ccctacgacg aggccctgct
     3181 gcggcgggaa atgaaccttt tcccgg
//
LOCUS       DASTPD010000184         5547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_707404, whole genome shotgun sequence.
ACCESSION   DASTPD010000184 DASTPD010000000
VERSION     DASTPD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707404"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFR57_06145"
     CDS             <1..489
                     /locus_tag="VFR57_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU0258982.1"
                     /translation="GACLVYDETGRRVARYDKMHLFHFEGENERYDEARTLEPGARAV
                     AIGSPFGRLALSVCYDVRFPELYRGLGEFDLMFVPSAFTVPTGAAHWETLLRARAIEN
                     KAYVVAPAQGGSHASGRRTWGHSMIVDPWGEVLAMREEGEGVVLAEIDSERIKDVRRR
                     LS"
     gene            522..1925
                     /gene="tldD"
                     /locus_tag="VFR57_06150"
     CDS             522..1925
                     /gene="tldD"
                     /locus_tag="VFR57_06150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEU0258983.1"
                     /translation="MLAPFELGAQKLERVFGTLAAHKVDYADLYFQYSRFEGWSLEEG
                     IVKSGSFNIEQGVGVRAISGEKTAFAYSDEISFNALQDAAKATRAIAAAGQARRFKAA
                     ARRSPVPLYRDVDPIASLDSQEKVTLLERLERKCRALDPRVTQVIASLGGEYEVVLIA
                     RADGAVSADVRPLVRLSVQVIAEANGRRESGSAGGGGRTDYSHFTDEVLDRYARQAVS
                     QALVNLEARPAPAGTMTVVLGPGWPGVLLHEAVGHGLEGDFNRKGSSAFSGRLGQRVA
                     SRGVTVIDDGTLPGRRGSLSVDDEGTPTQRTVLIEDGVLRGYMQDSLNARLLGMPLTG
                     NARRESFAHVTLPRMTNTYMLAGPHDPAEIIASVPKGIYATNFGGGQVDITSGKFVFS
                     AAEAYLIENGKVTHPVKGATLIGNGPDVLTRVTMIGRDLELDPGIGTCGKEGQSVPVG
                     VGQPTLKIEGLTVGGTA"
     gene            1978..2406
                     /locus_tag="VFR57_06155"
     CDS             1978..2406
                     /locus_tag="VFR57_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258984.1"
                     /translation="MRTLDRVSAEDAPLKLTVTGMPAAKCAKGHAAPIDRDFMLWLIQ
                     ELKSRGGALAAGEAKGMVFKKYTCACGKELESKSEKRQSFPLDLAYQGAPAFKAELDM
                     PVYRCTGCGKEQLRSTKELQGHTAQAIAALNDAAGFPHSG"
     gene            2378..3214
                     /locus_tag="VFR57_06160"
     CDS             2378..3214
                     /locus_tag="VFR57_06160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU0258985.1"
                     /translation="MRPDSRTAASELAALIADGARVALPPDYSMPAMAVVRALIRRRS
                     KNLRLLGVPVLGLCADMLIGAGCVAEVESSAVSLGEAGLAPRFSEAVEKGEIVVRDAT
                     CPAVHTALQASEKGVPFMPLRGVLGSDLLRGREDWKVLPNPFSGSEDPILFVRAIHPD
                     IALFHARWADEAGNVWVGRRRELATIAHASKACLVTYEEMKSGDMLEDELLAPGVISA
                     AYVSALAPAARGAWPLGIAGVYDIDDAHLAHYARAARTREGFQRYLDEFVCIPAKSYS
                     PA"
     gene            3319..3849
                     /locus_tag="VFR57_06165"
     CDS             3319..3849
                     /locus_tag="VFR57_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258986.1"
                     /translation="MSMLHKRKGNPFSEGSRELFDLAGQGRIDVFFLGGAQIDGQANI
                     NLVRAEGRRFPGSFGSAFMYSVVKRTILFREEHSPRVLVPRVEFISARGDPAALLTGK
                     ALFSWQKEKRRFRLESVHEPGDIRAETGFDYDVPESIPLTPAPSADELALLRGPVAKG
                     IAPDYPDFAKRVWGIH"
     gene            3854..4753
                     /locus_tag="VFR57_06170"
     CDS             3854..4753
                     /locus_tag="VFR57_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258987.1"
                     /translation="MNAPEKVKQFDRDAHAVGNIVHLEHFNVCIPDQRLATLFYVVGL
                     GGTRDPYLFMGLENMWVNFGRTQVHLPSRAVPPRTEVLRGTVGLVVPSLEDLLWRLEF
                     AKSEMKRVAPAIPNKFSFQKKGDVVEATCPWGNRVRCHAPSPQFGHIELGLVYVDFDV
                     PQGTADGIARFYREVMKAPSRMEQSRATVSIGKSQYLHFTETEQPLPEYDNHHIQIYI
                     ADFGSPYHWLKERELITMETDADEWRFQWIVDPRDQRKLFQIEHETRSMKHRLFNRPL
                     VNRNHGITNMTYVPGHDAFRGTF"
     gene            complement(4770..5120)
                     /locus_tag="VFR57_06175"
     CDS             complement(4770..5120)
                     /locus_tag="VFR57_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEU0258988.1"
                     /translation="MSELNDPRVLYAAERTLLAWSRTASGLMALGFFVDRAALMTEAA
                     KGGRGLAAAIGIAFLLLGVLLNILSVVQYRRGIASLRAAEIPSGYWPNLAVFTSLAVA
                     ALGLLLIVYLMLAR"
     gene            complement(5117..>5547)
                     /locus_tag="VFR57_06180"
     CDS             complement(5117..>5547)
                     /locus_tag="VFR57_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0258989.1"
                     /translation="RYGLKVSEADADHMNRAWHRLKARFIIAPCSNGNIALMVNLAKR
                     AGLPWDCILGAETARAYKPMPDAYLNSCRQLGLAPGSVMMVAAHNNDLKAAKAQGMKT
                     AFVARPTEHGPGQTTDLAADPACVDLPARDFVDLAVKLGA"
BASE COUNT         1002 a         1800 c         1860 g          885 t
ORIGIN      
        1 ggcgcctgcc tggtctacga cgagacgggc agacgggtcg cacgctacga caagatgcat
       61 ctctttcatt tcgagggcga aaacgagcgc tacgacgagg cccgcacgct cgagccgggc
      121 gctcgggcgg tcgccatcgg cagcccgttc gggcggcttg cactttcggt ttgctacgat
      181 gtgcgcttcc ccgagctcta tcgcgggctg ggggaattcg acctcatgtt cgtgccctcc
      241 gccttcaccg tgccgaccgg cgcggctcac tgggaaacgc tgctcagggc gcgcgccatc
      301 gagaacaagg cctacgtggt cgccccggcg cagggcggca gccacgcgag cggccggcgc
      361 acctggggcc acagcatgat cgtcgacccc tggggtgagg ttctcgccat gcgcgaggaa
      421 ggcgagggcg tggtgcttgc cgagatagac agcgaacgca tcaaggacgt aagaaggagg
      481 ctcagttgac tgcgcaggca ctgcgaaccg ccgaaaccac gctgctcgcg ccgttcgagc
      541 tgggggcgca gaagctcgag cgcgtgttcg gcacgcttgc tgcgcacaag gtcgactatg
      601 ccgaccttta cttccagtac tcgcgcttcg agggctggag cctggaggag gggatcgtca
      661 agtcaggcag cttcaacatc gagcagggcg ttggcgtacg cgccatctcg ggcgagaaaa
      721 ccgcgttcgc ctactcggac gagatcagct tcaacgcgct gcaggacgcg gcgaaggcta
      781 cgcgcgccat cgccgcggcg ggacaggcga gaagattcaa ggcggcggcg cggcgcagcc
      841 cggtgccgct ctaccgcgac gtcgacccga ttgcctctct cgattctcaa gaaaaagtaa
      901 ctctgttgga aagactggag cgcaaatgtc gcgcgctcga cccgcgggtc actcaggtga
      961 tcgcgagcct gggcggggag tacgaggtcg tgctgatcgc gcgcgccgac ggcgcggtct
     1021 cggccgacgt acggccgctc gtgcggctct cagtccaggt gatcgctgaa gcgaacggca
     1081 ggcgcgagag cggaagcgcc gggggcggcg gacgcacgga ctactcccac ttcaccgacg
     1141 aagtgctgga ccgctacgcg cgccaggcgg tttcgcaggc gctcgtcaat ctcgaggcgc
     1201 gccccgcccc cgccggcacg atgaccgtgg tgctcggccc cggctggccg ggggtgctgc
     1261 tgcacgaggc ggtgggccac ggcctcgaag gcgacttcaa ccgcaagggc agctctgcat
     1321 tctccgggcg cctcggccag cgggtggcct cgcgcggcgt caccgtgatc gacgacggca
     1381 cgcttcccgg ccggcgcggc tcgctcagcg tcgacgacga aggcacgccc acgcagcgca
     1441 ccgtgctgat cgaggacggc gtgctgcgcg gctacatgca ggacagcctg aatgcgcgcc
     1501 tgctcggcat gccgctcacg ggcaacgcgc gccgcgaatc gttcgcgcac gtcacgttgc
     1561 cgcgcatgac caacacctac atgctggccg gcccgcacga cccggccgaa atcatcgcca
     1621 gcgttccgaa gggaatctac gcgaccaatt tcggtggcgg ccaggtcgat attacgagcg
     1681 gcaagttcgt gttctcggcc gccgaggcct atctcataga aaacggcaag gtcacgcacc
     1741 ccgtgaaagg cgcgacattg atcggaaacg gcccggacgt cctgacccgc gttaccatga
     1801 tcgggcgcga cctcgagctc gatccgggca tcggcacctg cggcaaggag ggccaaagcg
     1861 tgccggtcgg ggtcggacag ccgacgctca agatcgaagg tttgacggtg ggaggaaccg
     1921 cataactgga ggaggcactt ggtaaagcac tgccctcgct gcagcaacgc cgtggaaatg
     1981 agaacgctcg accgcgtgag cgcggaagac gcgccgctga agctcacggt aaccggcatg
     2041 ccggccgcga agtgcgccaa aggccacgcg gcgccgatcg accgcgactt catgctgtgg
     2101 ctcattcaag agctcaagag ccgcggcggc gcgcttgcgg caggtgaagc gaaagggatg
     2161 gtcttcaaga agtacacctg tgcctgcggc aaggagctcg agtcgaagtc cgagaagcgc
     2221 cagtcgttcc cgctcgatct cgcctatcag ggcgcgcccg cgttcaaggc cgagctcgac
     2281 atgccggtct acaggtgcac cggctgcggc aaggagcagc tgcgctcgac gaaagagctg
     2341 caaggccaca ccgcgcaggc gatcgccgcg ctcaacgatg cggccggatt cccgcacagc
     2401 ggctagcgag cttgcggcgc tgatcgcgga cggcgcgcgg gtcgccctgc cgccggacta
     2461 ctccatgcct gcgatggcag tagttagggc tttaataaga agaagatcga agaacctgcg
     2521 cttgctcggc gtgccggtcc tgggtctgtg tgccgacatg ctcatcggcg cgggctgcgt
     2581 agccgaagtg gagtcgagcg cggtctcgct cggggaagcg gggcttgcgc cgcgcttcag
     2641 cgaagcggtg gaaaagggcg agatcgtcgt gcgcgatgcc acgtgtccgg ccgtgcacac
     2701 cgcgctgcag gcaagcgaga agggcgttcc cttcatgcct ctgcgcggtg ttctgggcag
     2761 cgacctcctt cgcggacgcg aggattggaa agtcttgccc aatccctttt cggggagtga
     2821 agatcccatc ttgttcgtgc gtgccataca ccccgacatc gcgctgttcc acgcgcgctg
     2881 ggcggacgag gccgggaacg tgtgggtcgg gcgcaggcgc gagctcgcga cgatcgctca
     2941 cgcgtccaag gcgtgtcttg tgacctatga agaaatgaag agcggcgaca tgctcgagga
     3001 cgagctgctc gctcccggtg tgatttccgc ggcgtacgtg agcgcgctcg cgccggcagc
     3061 gcgcggcgcc tggccgctcg ggattgccgg cgtctacgac attgacgacg cgcatcttgc
     3121 gcactacgcg cgcgccgccc gtactcggga aggctttcag cgctatctcg acgagttcgt
     3181 ttgcattccc gcgaagagct actcgccagc gtaattgcac ggctcattgg cgacgctcgg
     3241 cacgtcgccg tcggcgcagc ttcgcccatc cccgcaaccg gagtttttct tcataggcag
     3301 aagcaccccg ggatgcgggt gtcgatgctt cacaagagga aaggcaaccc cttcagcgaa
     3361 ggctcgcgcg agctcttcga cctcgcggga caggggcgga tcgatgtctt cttcctcggc
     3421 ggcgcgcaga tcgacggtca agcgaacatc aacctggtgc gcgccgaagg ccggcgtttt
     3481 cccgggtctt tcggctcggc cttcatgtat tcggtggtga agcggacgat cctcttccgc
     3541 gaggaacact cgccgcgcgt cctcgtgccg cgggtcgagt tcatctccgc gcgcggcgat
     3601 ccggccgcgc tacttacggg caaggcgctc ttttcctggc agaaggagaa gcgccgcttt
     3661 cgcctggaaa gcgtgcacga gccaggtgat atccgcgcgg aaacggggtt cgattacgac
     3721 gtccctgaaa gcattcccct gactccggct ccgtcggccg acgagctcgc cctgctgcgc
     3781 gggccggtgg ccaaggggat cgcgccggac taccccgatt tcgcaaagcg agtgtgggga
     3841 atacactagc gccatgaacg ccccggaaaa ggtcaagcag ttcgatcgcg acgcccacgc
     3901 ggtgggaaac atcgtccacc tcgagcactt caacgtgtgc atccccgacc agcgcctggc
     3961 gacgctgttc tacgtcgtcg gcctgggcgg cacgcgcgac ccgtatctgt tcatgggcct
     4021 ggagaacatg tgggtgaact tcggccgcac gcaagtgcac ctgccgagcc gcgccgtccc
     4081 gccgcgcacc gaagtgctgc gcggcaccgt gggcctcgtg gtgccgagtc ttgaggatct
     4141 cctctggcgg ctcgagttcg ccaagagcga gatgaagcgc gtcgcgcccg caattccgaa
     4201 caaattctcc ttccagaaaa agggcgacgt cgtcgaagcg acctgcccgt ggggcaaccg
     4261 cgttcgctgc cacgcgccgt cgccgcaatt cggccacata gagctcggcc tggtgtacgt
     4321 ggacttcgac gttccacagg ggaccgccga cggaatcgcg cggttttacc gcgaagtgat
     4381 gaaagcacct tcgaggatgg aacagagccg cgccaccgtc agcattggca agagccaata
     4441 tcttcacttc accgaaacgg agcagccgct gccggagtac gacaaccacc acatccagat
     4501 ctacatcgcc gacttcggca gcccgtacca ctggctgaag gagcgggagc tgatcacgat
     4561 ggaaacggac gccgacgagt ggcgcttcca gtggatcgtc gacccgcgcg accagcgcaa
     4621 gctcttccag atcgagcacg agacgcgcag catgaagcac cgcctgttca accggccgct
     4681 cgtcaaccgc aaccacggca tcacgaatat gacgtacgtg ccgggtcacg acgccttccg
     4741 cggcacattt tgaacacgcg cagccggccc taacgcgcga gcatcaggta aacgatcaga
     4801 agcagcccca gcgccgcgac cgcgaggctg gtgaagacgg ccaggttcgg ccagtatcct
     4861 gagggaatct cggcggcacg aagcgacgct atgccgcggc ggtactgtac gaccgagagg
     4921 atgttcagaa gcactccgag gagaaggaaa gcgatgccga tggcggcggc gaggccgcgg
     4981 ccgcccttcg ccgcctcggt catgagcgcg gcacggtcga cgaagaagcc gagcgccatc
     5041 agtccggacg cggtgcggct ccaggcgagc aacgtgcgct cggccgcgta aaggacccgc
     5101 gggtcgttga gctcgctcat gcgccgagct tgaccgcgag gtctacgaaa tccctggcgg
     5161 gcaggtcgac gcaggccgga tccgccgcga gatcggtcgt ctgccccgga ccatgctcgg
     5221 tcggccgcgc gacgaatgcg gtcttcatgc cctgcgcttt cgcggccttg aggtcgttgt
     5281 tatgcgccgc gaccatcatc accgaccctg gcgcaaggcc gagctggcgg catgaattca
     5341 ggtaggcatc gggcatcggc ttgtaagccc gcgcggtttc cgcgcccagg atgcagtccc
     5401 acggcagccc ggcgcgcttg gcgaggttca ccatgagcgc gatgttgccg ttggagcacg
     5461 gcgcgatgat gaacctcgct ttgagccggt gccaggcgcg gttcatgtgg tccgcatcgg
     5521 cttccgagac tttcaatcca taccggg
//
LOCUS       DASTPD010000185         5242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1115304, whole genome shotgun sequence.
ACCESSION   DASTPD010000185 DASTPD010000000
VERSION     DASTPD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115304"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFR57_06185"
     CDS             complement(<1..196)
                     /locus_tag="VFR57_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0258990.1"
                     /translation="MKLKSPTALAVVSLCAALPLNVQSAPTVSFSAPTNGGTVSGTLS
                     GTACQLISGSSLYRVRFFVDS"
     gene            610..1239
                     /locus_tag="VFR57_06190"
     CDS             610..1239
                     /locus_tag="VFR57_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258991.1"
                     /translation="MRILIADDHPIVRHGLKQMLAGDPSATVVGEATNGDEALELARK
                     VEWDMAIFDFTMPGRSGLDLLGDMKKEFPGRPVLILSMHSEDVHGSRVLRGGAAGYIT
                     KDSAPQELATAVRKVANGGKYVSASLAEILASECSSDPQKALHEKLSDREYRVMWLLA
                     TGKQISEIAKEMGLSPSTVSTYRTRILRKLGLTSNAALVHYAVRHQLVG"
     gene            1236..2351
                     /locus_tag="VFR57_06195"
     CDS             1236..2351
                     /locus_tag="VFR57_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0258992.1"
                     /translation="MNDVAQFHDLHAEQRSEVEQVEKAKILLVDDEPKSLFALQELLS
                     TLGQNLMVAQSGEEALRLALKNDFAVILLDVRMPGIDGFETARLIRSRERSKSTPIIF
                     LTAAADEMTSMFRGYEVGAVDYLMKPVIPEVLKSKVAVFVELFRKSERLRESEDKLRR
                     LAAHLISIREEERAHIAREIHDELGQVLTGLKMEVTWLAKRLKDKPLLDKTDSMCALI
                     DSSVQTVRKIATGLRPEMLDDMGLVAAVGWQAKEFQKRTGIRCRLKLPAETKFDIDIS
                     TTMFRIFQEILTNVARHARATRVDIELALRDSEVLLDVVDNGVGIQDTELTGKKSLGL
                     LGMQERALLFGGDVKITGSPGHGTRVSVNIPLSRKAK"
     gene            2348..2977
                     /locus_tag="VFR57_06200"
     CDS             2348..2977
                     /locus_tag="VFR57_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0258993.1"
                     /translation="MKVLIADDHPVVRQGLRQMLAVESDLTVVGEARNGQEVVELSRR
                     VPWEVAVLDYNMPGKNGLELIKELRQRYPGRAVLVLSMYPEDRYAVRALKAGAAGYLT
                     KESAPEELVSAIRKVAGGGRYVTPTLGEKLALELEDNRGKPLHETLSDREYQVMWMIA
                     SGKTVRQIADELFLSPNTVSTHRTRILRKMNMKSNAELMHYAIAHHLVD"
     gene            3107..>5242
                     /locus_tag="VFR57_06205"
     CDS             3107..>5242
                     /locus_tag="VFR57_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU0258994.1"
                     /translation="MDSIQAGLQMMLDRGELLKALRSFKRGDFSVRMPLNLTGIDGEI
                     AQTFNDVVELEEAKAAEIARVADLVGKEGQTASRMSVPGASGAWLEVIESVNTLINDL
                     SYPMTEVARVIGSVAKGDLSHTMVLEADGRPLRGEALRLGTVVNTMVGQLNAFASEVS
                     RVAREVGTEGKLGGQAQVAGVAGVWKDLTDNVNSMAANLTGQVRNIADVTTAVARGDL
                     SKKITVEVKGEILELKNTVNTMVDQLNAFASEVSRVAREVGTEGKLGGQAQVRGVGGV
                     WKDLTDNVNSMAANLTGQVRNIAEVTTAVAKGDLSKKITVDVKGEILELKSTINTMVD
                     QLNAFAGEVSRVAKEVGTDGKLGGQAQVAGVGGVWKDLTDNVNQLAANLTGQVRNIAE
                     VTTAVAKGDLSKKITVEVKGEVLELKSTINTMVDQLNAFAGEVSRVAREVGTDGKLGG
                     QAQVKGVAGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVAKGDLSKKIGVDVKGEVLE
                     LKNTFNTMVDQLNAFASEVSRVAREVGTEGKLGGQAQVAGVAGVWKDLTDNVNSMAAN
                     LTGQVRNIAEVTTAVAKGDLSKKITVDVKGEILELKSTFNTMVDQLNAFAGEVSRVAR
                     EVGTEGKLGGQAQVIGVAGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVAKGDLSKKI
                     TVDVRGEILELKSTINTMVDQLNAFASEVS"
BASE COUNT         1086 a         1594 c         1729 g          833 t
ORIGIN      
        1 tgctatcgac gaagaaccgc acgcgataga ggctggagcc gctaatcagc tggcacgcgg
       61 tgccgctaag cgtgccggag acggtgccgc cgttggtggg cgcgctgaaa gaaaccgtag
      121 gcgcggactg cacattcaac ggtagcgccg cgcaaagcga aacgaccgcg agcgcggtcg
      181 gtgacttgag cttcatggac aacccctccc tttcttggga atggactaca cgggtcgcct
      241 tcgagcagaa gccatgccac cgaatgcact acaaggttaa tcaacggctt aactacatac
      301 cgataggagg aagtgtgaca aatgtgtagc agccgcacga cgccacacgc agtgaaaacc
      361 ccaagtcact gtttccgcac gcgaagaaat gtcgccgtga gccgactttg cggtgcgtcg
      421 ccgaatttcg gcagaggaga atgtgcgcgc tacacgtgcg cgcgtggcct actgcgcgat
      481 gggtgtcggg acgcgaccta caagcgtgtc aactccttgc tacatcgaaa aaatttaggc
      541 gtgacatcgg tggtcaagcg aattgcgtgt gcatatactt ttcgctcacg ttaaggcagg
      601 gagaatttca tgcggatact catcgcagac gatcacccga tcgtccgaca cggcttgaag
      661 cagatgctcg cgggcgaccc ctcggcaacc gtcgttgggg aagcgacaaa cggcgacgag
      721 gcgttggaac tcgcgcgcaa ggtggagtgg gacatggcga tcttcgactt caccatgccc
      781 ggacgtagtg gtctggacct cctcggcgac atgaagaagg agtttcccgg ccgcccggta
      841 ctcatcctgt cgatgcattc ggaggatgtg cacggcagcc gtgtgctgcg cggtggcgcg
      901 gccggctaca tcaccaagga cagcgcgccg caggagctcg cgaccgcggt gcgcaaggtc
      961 gcgaacggcg gcaagtacgt gtccgcgtcg ctcgcggaaa ttctcgcttc cgagtgctcg
     1021 agcgatccgc agaaagcgct ccacgagaag ctctcggatc gcgaataccg cgtgatgtgg
     1081 ctcctcgcca ccggcaagca gatcagcgag atcgcgaagg agatgggctt gagcccgtcg
     1141 acggtgagca cctatcgcac gcgcatcctg cgcaagcttg ggcttaccag caacgcggcg
     1201 ctcgtgcact acgcagtccg ccaccagctg gtcgggtgaa cgacgtagcg caatttcacg
     1261 acctgcacgc ggagcagcgc tccgaggtcg agcaggtcga aaaggcgaag attttgctcg
     1321 tcgatgacga gccgaagagc ctgttcgcgt tacaggagct gctctccacc ctgggccaga
     1381 acctgatggt ggcgcagtca ggcgaggagg cgctgcgcct cgcgctcaag aacgatttcg
     1441 cggtgatcct gctcgatgtg cgcatgcccg ggatcgatgg cttcgagact gcgcgcctca
     1501 ttcgttcgcg cgagcgctcg aagtccacgc cgatcatctt cctcaccgcc gcggccgacg
     1561 agatgacgtc gatgttccgc ggctacgaag tcggcgcggt ggattacctg atgaagccgg
     1621 ttatccccga ggtgctgaaa tccaaggtgg cggtgttcgt cgagcttttc aggaagagcg
     1681 agcggctgcg cgaatccgaa gacaagctcc ggcgccttgc ggctcacctg atcagcattc
     1741 gggaagagga acgggcgcac atcgcgcgcg agatccacga cgagctcggc caggtgctca
     1801 ccggactcaa gatggaagtc acctggctcg ccaagcgcct gaaggacaag ccgcttctcg
     1861 acaagaccga ctcgatgtgc gcgctcatcg actccagtgt gcagaccgtg cgcaagattg
     1921 cgacggggct tcgtcccgag atgctcgacg acatgggcct tgtcgccgcg gtcggctggc
     1981 aggcgaagga attccagaaa cgcaccggca tccgctgccg gctcaagctt cccgcggaga
     2041 ccaagttcga catcgacatc tccaccacga tgttccgcat cttccaggag atcctcacca
     2101 acgtcgcccg ccatgcgcgg gcgacgcgcg tggacatcga gctcgcgttg cgtgacagcg
     2161 aggtgctgct ggacgtcgtc gataacggcg tgggtattca ggacaccgag ctcaccggca
     2221 agaagtccct tggcctgctc ggcatgcagg agcgcgcgct ccttttcggc ggcgacgtca
     2281 agatcaccgg ctcgccgggc cacggcacgc gcgtatcggt gaacatccca cttagcagga
     2341 aggccaaatg aaagtgctca tcgccgatga ccatccggta gtgcgccagg ggctgcggca
     2401 gatgctcgcc gtcgagtccg acctcaccgt cgtcggcgag gcgcgcaacg gccaggaagt
     2461 ggtcgagttg tcgcggcgcg tgccgtggga agtcgcggtg ctcgattaca acatgccggg
     2521 caagaacgga ctggagctca tcaaggagct gcgccagcgc tatcccgggc gcgcggtgct
     2581 cgtcctgagc atgtaccccg aggaccgcta cgcggtgcgg gcgcttaaag cgggagcagc
     2641 cggctacctc acgaaggaat cggcgccaga ggaactcgta agcgcgatcc gcaaagtcgc
     2701 cggcggcggg cgctacgtca cgccgacgct cggcgagaag ctcgcgctcg agctcgagga
     2761 caaccgcggc aagccgctcc acgagaccct gtcggaccgc gagtaccagg tgatgtggat
     2821 gatcgcttcc ggcaagacgg tgcggcagat cgccgacgag cttttcctat cgcccaacac
     2881 cgtcagcacg catcgcacgc gcatcctgcg caagatgaac atgaagtcga acgcggagct
     2941 gatgcactac gcgatcgcgc accatttggt ggactgaaaa aatcgggctc tgaccccgat
     3001 ttctggtttg tagtaaatca actacacgaa cgcgggggct ttcgcgatag ccgctgtccg
     3061 ggtccgcccc tacgatttca ctgttttccc ttattagaac aacgctatgg actccatcca
     3121 agccggactc cagatgatgc tcgatcgcgg tgagctcctg aaggcactgc gctcgttcaa
     3181 gcgcggcgac ttctcggtgc gcatgccgct caacctcacg ggcatcgacg gcgaaatcgc
     3241 ccagaccttc aacgacgtgg tcgagctcga ggaggccaaa gcggccgaga tcgcgcgcgt
     3301 cgccgacctc gtcggcaagg aaggccagac cgcgagccgc atgagcgttc ccggggcgag
     3361 tggcgcgtgg ctcgaggtca tcgagtccgt caatacgctt atcaacgacc tctcctatcc
     3421 gatgaccgag gtcgcgcgcg tgatcggctc ggtggcgaag ggcgatctct cgcacaccat
     3481 ggtgctcgag gccgacgggc gtccgctgcg cggcgaagcg ctgcgcctcg gcacggtggt
     3541 caacacgatg gtgggacagc tcaacgcgtt cgcctccgag gtctcgcgcg tggcgcgcga
     3601 ggtgggtacc gaaggaaagc tcggcgggca ggcgcaggtt gccggcgtgg ctggcgtgtg
     3661 gaaggacctc acggacaacg tgaactccat ggcggcgaac ctcaccggcc aggtgcgcaa
     3721 catcgccgat gtcacgaccg cggtcgcgcg cggcgacctc tcgaagaaga ttaccgtcga
     3781 ggtaaagggc gagatcctcg agctcaagaa caccgtgaac acgatggtgg accaactgaa
     3841 cgcgttcgcc tccgaggtgt cgcgcgtagc gcgcgaggtc ggcacggaag gaaagctcgg
     3901 cggccaggcg caggtgcgcg gcgtgggcgg cgtgtggaag gacctcaccg acaacgtgaa
     3961 ctccatggcc gcgaacctga ccggccaggt gcgcaacatc gccgaggtca ccaccgcggt
     4021 ggcgaagggc gatctttcca agaagatcac ggtggatgtg aagggcgaga ttctcgagct
     4081 gaaaagcacg atcaacacga tggtcgatca gctcaacgcc ttcgccggcg aggtgtcgcg
     4141 tgtggcgaag gaagtgggaa ccgacgggaa gctcggcgga caggcgcagg tcgccggcgt
     4201 cggcggggtc tggaaggacc tgaccgacaa cgtgaatcag ctcgcggcga acctcaccgg
     4261 ccaggtgcgc aacatcgccg aggtcaccac cgcggtggcg aagggcgatc tatccaagaa
     4321 gatcacggtg gaagtgaagg gcgaggtgct ggagctcaaa tccaccatca acaccatggt
     4381 ggatcaactc aatgccttcg cgggcgaagt atcgcgcgtg gcgcgcgagg tcggcaccga
     4441 cggcaagctc ggcggccagg cgcaggtgaa aggcgtcgcc ggcgtatgga aggacctcac
     4501 ggacaacgtg aactccatgg ccgccaacct taccggccag gtgcgcaata tcgcggaggt
     4561 caccaccgcg gtggcgaagg gcgatctctc gaagaagatc ggggtggacg tgaagggcga
     4621 ggtcctcgag ctcaagaata cgttcaacac catggtcgac cagctgaatg ccttcgccag
     4681 cgaagtgtcg cgcgtggcgc gcgaggtcgg caccgaagga aagctcggcg ggcaggcgca
     4741 ggttgccggc gtcgccggcg tatggaagga cctcaccgac aacgtgaact ccatggccgc
     4801 gaacctcacc ggccaggtgc gcaacatcgc cgaagtgacc accgcggtcg ccaagggcga
     4861 tctctccaag aagatcaccg tcgacgtgaa gggcgagatc ctcgagctca agtcgacgtt
     4921 caacacgatg gtcgaccagc tgaacgcctt cgcgggcgaa gtgtcacgcg tggcgcgcga
     4981 ggtgggcacc gagggcaagc tcggcggcca ggcacaggtc atcggcgtcg cgggtgtctg
     5041 gaaggacctt accgacaacg tgaactcgat ggccgcgaac ctcaccggtc aggtgcgcaa
     5101 catcgcggag gtcaccaccg cggtggcgaa gggcgacctc tcgaagaaga tcacggtcga
     5161 cgtgaggggc gaaattctcg agctgaaaag caccatcaac acgatggtcg accagctgaa
     5221 cgcgttcgcc tccgaggtgt cg
//
LOCUS       DASTPD010000186         8732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1728812, whole genome shotgun sequence.
ACCESSION   DASTPD010000186 DASTPD010000000
VERSION     DASTPD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728812"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="def"
                     /locus_tag="VFR57_06210"
     CDS             complement(<1..330)
                     /gene="def"
                     /locus_tag="VFR57_06210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0258995.1"
                     /translation="MSRLNILRYPDARLHKVAAPVTVFDDGLKRLVADMVETMHAAPG
                     IGLAAIQVDVAKQVIVVDVSERRDSLVVLVNPEILEATGVSDIEEGCLSVPGIYELVE
                     RAERVKVR"
     gene            502..1584
                     /locus_tag="VFR57_06215"
     CDS             502..1584
                     /locus_tag="VFR57_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU0258996.1"
                     /translation="MISANVAAQAPGAPASAPLTIKPDAPDRYVVVPGDTLWGISERY
                     TDSPWRWPELWNMNREQIRNPHLIYPGYVILLDRDRARLTIAPPGTETKPATSGEPGA
                     PTTAPGTVKLSPRLRAESLARQEIPSIPASVIEPFLSRPLVIEPDGLEKAPTIIATQQ
                     DRVVLAAGNSAYVRGIGASKDETWYVYRKGTALVDPDTNKTLGYEAIFLGTAQLTRPG
                     EPATVVLTSAVQEVGAGDKLVAAGRPQPVNYAPHAPGTQIKGRVIGIYGGVAHVGEAG
                     PQSIISINRGKADGVEVGHVLALYNRGGTVRDRSKPRNAPDARIQLPDERAGLAFVFR
                     VFDRISYALIMQVTRPVSPLDVVQTP"
     gene            1591..2682
                     /gene="dprA"
                     /locus_tag="VFR57_06220"
     CDS             1591..2682
                     /gene="dprA"
                     /locus_tag="VFR57_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0258997.1"
                     /translation="MNPDPGLASWLQLALTPGLGPATFQRILRQFGLPQVALAKSRAE
                     LASFTTPAVLAALDSNAVVDAVARSLAWAALPGCSIVTLADEEYPRLLLEIPDPPPLL
                     YAHGRTELLAGPALAIVGSRNSTQQGEANAESFARALSDAGFTIISGLALGIDAAAHR
                     GGLAGASSTIAVLGTGVDVAYPSRNAALAAHIAKRGLLITEFPLGTPPAAQNFPRRNR
                     LISGLARGCLVVEAALASGSLITARAAAEQGREVFAIPGSIHSPLSKGCHALIKSGAK
                     LVESAEDVLTELSGFRQSASATTTSNAASPQDSGLLACMGHDPVDVDSLCSRAGLSAE
                     QVSSELLRLELDGRIAALPGGLFQRLEKG"
     gene            2734..3192
                     /locus_tag="VFR57_06225"
     CDS             2734..3192
                     /locus_tag="VFR57_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEU0258998.1"
                     /translation="MYDVLVFLFENCQQAELAYDQERVARKLSAAGFEDSDISEALHW
                     LAGMTHTPRSVREPLPDARSSFRAYAPRELAKLDAECRGFLLTLEHSGILSAETRELA
                     IERALAASGEKLNLEQLKLVVLMVLWNQKTPTSRLVAEDLFSTPHARLAS"
     gene            3281..5743
                     /locus_tag="VFR57_06230"
                     /pseudo
     CDS             3281..5743
                     /locus_tag="VFR57_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(5736..8138)
                     /gene="gyrB"
                     /locus_tag="VFR57_06235"
     CDS             complement(5736..8138)
                     /gene="gyrB"
                     /locus_tag="VFR57_06235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU0258999.1"
                     /translation="MGEAEKNYDSDAIKVLKGLEAVRKRPGMYIGDTSDGTGLHHMVF
                     EVVDNAVDEALAGFCDEIVATIHTDNSVSVLDNGRGIPTGVKDDDTLKRSAAEIVMTE
                     LHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTIWREGRVHQIEFRNGVAVAPLA
                     ATGATERRGTEVHFLASKETFGNVDYHYDILARRLRELSFLNNGVKIVLIDQRAGKEE
                     NFAFSGGVKGFVEYMNRAKNVLHPNVFLGHGVKDGITVEVAMQWNDSYQENVQCFTNN
                     IPQKDGGAHLTGLRAAMTRVLNKYIEEHEIAKKAKVETTGDDMREGLACVLSVKVPEP
                     KFSSQTKEKLVSSEVRPVVEEIVAERLREFLDERPHDARTITVKIVEAAAAREAARKA
                     REMTRRKGVLDSMGMPGKLADCQERDPAKSELYLVEGDSAGGSAKQGRDRKFQAILPL
                     KGKILNVERARFEKLLSSAEIATLITALGCGIGAEEYNPDKLRYHRIIVMTDADVDGS
                     HIRTLLLTFFYRQMRELVERGHIYIAQPPLYKARLGKEERYLKDEHELNAFMLRQALV
                     DAALHPGAGRDPIGGDTLAELAKSYLLAEAVMERVSRLVDREVLQAMLGGVQMDLSRR
                     DTAEASAEALEAVIGGEGFAVAPHFDQKTERYQVKVERTRHGNVRMSTIDTEFVHSGD
                     YAQIRQTAEMLRGLIGPGASVQRGEKRQPVRDFAQAMKWLLGEVEKAMTLQRYKGLGE
                     MNPAQLWETTMDPATRRLLKVQIEDGIAADEIFTKLMGDEVEPRRAFIESNALGVRNL
                     DV"
     gene            complement(8139..>8732)
                     /gene="dnaN"
                     /locus_tag="VFR57_06240"
     CDS             complement(8139..>8732)
                     /gene="dnaN"
                     /locus_tag="VFR57_06240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU0259000.1"
                     /translation="VATDGHRLAYAALKLGAQLPRQEVIVPRKTVLELGKLLADSDAP
                     VRIEIAATQAAFSFGAVELVSKLIDGKFPDYTRVIPTSHKNRLHAAREPLRQALQRAA
                     ILSNEKFRGVRWVLGDGSLKIVSSNAEQEEAQEELDVKYSGDGLDIGFNVNYLLDVLN
                     NVSGDEIECAFGDAASSALITYGAEKDFKYVVMPMRI"
BASE COUNT         1556 a         2886 c         2724 g         1566 t
ORIGIN      
        1 gcgcaccttc acgcgctcgg cacgctcgac gagctcgtag attccgggaa ccgaaagaca
       61 gccctcctca atgtcggaga ccccggtcgc ctccagaatc tccggattga ccagcacgac
      121 cagagagtca cgccgctccg aaacatcgac cacgatcacc tgcttagcaa cgtctacctg
      181 gatcgccgca agcccgatac cgggcgcggc gtgcatggtt tccaccatgt cggcaaccag
      241 cctcttcagg ccgtcatcaa aaaccgtgac cggcgcggcc accttatgca ggcgggcgtc
      301 gggatagcga agtatgttca aacgggacat ttcacttgca attgaatgga ttatgtggac
      361 aatctcaggc agcgtataag aaatcgaacc tttgtgctac attggggcgg aaaaaccaat
      421 ttccagccgg tccaagaacc gcgaaaggga cggatcgcca tggtccagaa agcctgtaag
      481 tctatcatag cgctcgcttt cttgatctcc gcaaacgttg cagcgcaggc acccggagcg
      541 cccgcatcgg cgccgctcac gatcaagcca gacgctcccg accgctacgt cgttgtcccg
      601 ggcgacaccc tttggggcat ctccgagcgc tacaccgact ctccttggcg ttggccggaa
      661 ctctggaaca tgaaccggga gcagatccgg aacccgcacc tcatctatcc cggctacgta
      721 attctgctcg accgcgatcg tgcgcggctg accattgcgc ctcccggtac ggagacgaag
      781 cccgcaacat ccggtgagcc gggagcaccg acgacggctc ccggcactgt caagctcagc
      841 ccgcgcctgc gcgccgaatc gctcgcgagg caggaaattc cgagtattcc ggcgtcggtg
      901 atcgagcctt tcctgtcacg gccgttggtc atcgaacctg acggactcga gaaagccccg
      961 acaatcatcg cgacacagca ggaccgcgta gtgcttgcgg caggcaactc cgcttacgtg
     1021 cgcggcatcg gcgcgtccaa ggacgagacc tggtacgtct accgtaaggg cactgcgttg
     1081 gtggatccgg ataccaacaa gactctcggg tacgaggcga tatttctcgg cactgcgcag
     1141 ctcacgcggc ccggcgagcc ggcgactgtt gtcctgacca gtgcggttca agaagtagga
     1201 gccggcgaca agttagtcgc ggccgggcgg ccacaaccgg tgaactatgc gccgcacgcg
     1261 ccgggtacgc agatcaaggg gcgtgtaata ggcatttatg gcggcgtggc gcacgtcggc
     1321 gaagccggcc ctcagtcgat catcagcatc aaccgcggca aggccgacgg cgtcgaagtg
     1381 ggtcacgtac tcgcgctgta caaccgcggt gggacggtgc gcgaccggag caagccgaga
     1441 aacgctcctg acgcgcgcat ccagctgcct gacgagcgcg ccggactcgc gtttgtgttc
     1501 cgcgtcttcg accggatatc ctacgcgctc atcatgcaag tcacgagacc ggtcagccca
     1561 ctcgatgtgg tgcagacacc gtaacaagaa gtgaatcccg acccggggct tgcgagctgg
     1621 ctgcagctgg cgctaacccc gggtctcggc ccggcaacgt tccagcgcat tcttcggcaa
     1681 ttcgggctgc cgcaagtcgc ccttgcgaag agccgcgccg agctcgcttc gttcaccaca
     1741 ccggccgtgc tggccgcgct ggactccaac gccgtcgtcg atgcggtggc ccgctctctc
     1801 gcctgggcag cgctgccagg ctgctcgata gtgacactcg ccgacgaaga gtatccccgg
     1861 ttactgctcg agattcccga cccgcctcct ttgctctatg cgcacggccg aaccgagctg
     1921 ctcgccgggc cggcgctcgc cattgtcggc agccgcaatt ccacacagca gggcgaagca
     1981 aacgccgagt catttgcgag ggcattgagc gacgccggtt tcacgatcat ttccgggctg
     2041 gcgctcggca tcgacgccgc ggcgcatcgt ggcggacttg cgggcgcctc gtccaccatt
     2101 gccgtcctcg gtaccggcgt ggacgtggcc tatccgagcc gcaacgccgc actcgccgcg
     2161 cacatcgcca agcgcggctt gctgattacg gagtttccgc tcggcacccc gccagcggcg
     2221 cagaactttc cgcgccgaaa ccgactgatc agcgggcttg cccggggttg ccttgttgtc
     2281 gaagccgcac tcgcgtctgg ctcgctgatt accgcgcgcg cggccgccga gcagggtcgc
     2341 gaggtgttcg ccataccggg ctcgatccac tccccgctct ccaagggctg ccacgcgctc
     2401 atcaagtctg gcgcgaagtt ggtcgaatca gccgaagacg ttctcaccga gctatcggga
     2461 ttccggcaga gtgcctcagc gaccactacc tcgaacgccg cgtcgccgca agattctggg
     2521 ctcctcgcgt gcatgggaca cgacccagtc gacgtggatt cgctgtgctc acgggccggg
     2581 ctctcggccg agcaggtttc gtcggaactc ttgcgcctgg aactcgacgg ccgcatcgcc
     2641 gcgctgcccg gcgggctttt ccagaggctc gagaagggtt agcgccggta aacgcaatcc
     2701 tcatcgtcgc gttataagaa agaaggaaca accatgtacg acgtcctcgt ttttctgttc
     2761 gagaattgcc agcaagcgga gctcgcctac gatcaagaac gtgtggcgag aaagctctcg
     2821 gcggccggat tcgaggattc ggatatcagc gaagcgctgc actggctcgc gggcatgacg
     2881 cacacgccgc gcagcgtgcg cgagccctta cccgacgcac gctcgagctt tcgcgcctac
     2941 gcgccgcgcg agctggcgaa gctggacgcc gagtgccgcg gcttcctcct cacgctcgag
     3001 cactcgggca tccttagcgc ggaaacgcgc gagctcgcga tcgagcgcgc gctggctgcg
     3061 tcgggcgaga agctcaacct cgagcagctg aagctggtcg tgctgatggt gctgtggaac
     3121 cagaagacac cgaccagccg actggtcgcg gaggatcttt tttccacgcc ccacgcgcgc
     3181 ctcgcgagct agcagctccc cgccgcattt tctcacacgc gcgcagggtg gttgaagcgc
     3241 gcgcgcgttt gcacttatca tctgcggcct ttcacgccgc atggcaaaga aactcatcat
     3301 tgcggagaaa ccgtcggtcg cgaccgacat cgctcgcgcg ctcggcggct tcaagcgcaa
     3361 cgccgattac ttcgagagtg agcgctacgt gctatcgtcg gcggtcgggc atttgctcga
     3421 gctttccgtc cccgaggagc atgacgtaaa gcggggtaag tggtcgttca gccgcttgcc
     3481 ggtcatccca ccgcaattcg cactcgcgcc gatcgagaag tcggcgactc gcctgagcct
     3541 cctgctgcgc ctgatgaagc gcaaggatgt caccggcctg gtgaacgcct gcgacgccgg
     3601 acgcgagggc gaactcatct tccgttacat cgtgcagcac gcgcgcaaca agaagcccat
     3661 cgagcgtcta tggcttcagt cgatgacgcc gcaggcgatc cgtgagggct tcgagcgctt
     3721 gcgctcagac aaggaaatgc tgccgctggc ggacgcggcg aagagccgct cggaggccga
     3781 ctggctggtc ggcatcaacg gcacgcgcgc catgacggcg ttcaactcaa aggagggcgg
     3841 cttttatctc accacagtcg gccgcgtgca gacgccgacg ctcgccatcc tggtcgagcg
     3901 cgaggagcgg atccgcgcct tcacgccgcg cgcctactgg gaagtccacg cccgctttac
     3961 tgccgaagcg ggcgagtacc ccgcgcgctg gttcgatccc aagttcaaga aggacgacga
     4021 tggcgagcgc aagcccgagc ggctatggag ctcgcaagaa gccgacgcca tcgtcgcggc
     4081 ctgtcgcggc aagacgggca ttgcgtcgga ggaaaccagg ccttcgacgc agctctcgcc
     4141 gcttctttac gacctcacta gcctgcagcg cgaagcgaac gcgcgcttcg ggttcccggc
     4201 ccgcatgacg ctcgcgcttg cgcaagccct ttacgagcgt cacaaggtcc tcacctatcc
     4261 gcgcaccgac gcgcgtgcgc tgcccgagga ctatctcggt acagtgaagc ggacgcttga
     4321 gacgctaggt gatgcgcgcg agttcgcgac gttcgcaaag catattctga agcacggctg
     4381 ggtgaagccg aacaagcgag tcttcgatga cagcaagatc tccgaccact ttgccatcat
     4441 cccgacgccg cagacgccgc gcaatttgaa cgaggcggag cagaagctct atgacatggt
     4501 ggcgcggcgc ttcctcgccg ttttctatcc cgccgccgag tacctgcaga ccacgcgcgt
     4561 cacccgggtg ggggaacatc acttcaagac cgaaggcaag gtgttgcagg tgccgggttg
     4621 gctggcggtc tatggacgtg ccggcggcga ggagagcgag aacctgccgc caatcgccga
     4681 cggcgagaaa gtctcggtgc gcgagattgc cgcgcacgcc ggcgagacca ggccgcccgc
     4741 gcgctatacc gaagccactt tgctctcggc gatggaaggt gctggcaagc tggtcgagga
     4801 cgacgagcta cgcgcggcga tggaagcgaa ggggctgggt acgccagcga cccgggccgc
     4861 gactatcgag ggcctgattc gcgaagacta catgcaccgc gagggccgcg agctcgtgcc
     4921 cacgcccaag gccttctccc tgatgttcgc gctgcacatg ctgcacatcc tcgagctggg
     4981 ctccccggag ctcacgggcg aatgggagca caagctgaag ttgatcgagg ccgggaagct
     5041 gacgcgagaa gagttcatgc accggatccg cgacctggtg aggaatgtgg tgagcaccat
     5101 taagcacggc gagatacctg acgtcgtgta cgccaccgta cccgcgccgt gcccgaagtg
     5161 cggaggcgtg gtgcaggaaa actaccgcaa ggtgcagtgc cagaagtgcg atttctctct
     5221 ctggcgcgtc atttcgggac gcgagtggac gccggaggag ctagcggaac tcttctcgaa
     5281 gcgcttcgtc ggccctctca acggcttccg cagcaagcag ggccggccct tcagcgccgg
     5341 catccggctc accgacgatc tgcgtctaga gtttgatttt ggccagggca cggcggccga
     5401 ggaggatgag gtcgacttca gcggccagga gccactcggc agctgtccga aatgcggcgc
     5461 gcgcgtgttc gagcatgggg tggcgtacgt ctgcgagaag gcaatcggtc gggagcgcag
     5521 ctgcgacttc cgctccggcc gcatgatcct gcagcagcca atcgagcgct tgcagatgca
     5581 gaagctgctc gacacgggcc gcaccgatct tctcaccggc ttcgtctcca agaaaggccg
     5641 ccggttcaag gctttcttgg taaagcagcc cgacggaaag atcggtttcg agttccagcc
     5701 gcgggcgccg aaagaaaagg cacccgagga gaaggctaca cgtcgaggtt ccgaacgccg
     5761 agcgcgttag attcgatgaa ggcgcggcgc ggctcgactt cgtcgcccat cagcttggtg
     5821 aaaatctcgt cggcggcgat accatcctcg atctgaacct tgagcagcct gcgtgtggcc
     5881 gggtccatcg tggtctccca cagctgcgcc gggttcattt cccccagtcc cttgtagcgc
     5941 tgcagggtca tcgccttctc gacttcgcca aggagccact tcatcgcctg agcgaagtcg
     6001 cggaccggct gccgcttctc gccgcgctgc acgctcgcgc caggtccgat cagcccgcgc
     6061 agcatctccg cggtttggcg gatctgcgcg tagtcgccgg agtgtacgaa ttcggtatcg
     6121 atggtgctca tgcgcacatt gccgtgccgg gtgcgctcga ctttcacctg gtagcgctcg
     6181 gtcttctggt cgaagtgcgg cgccaccgca aacccttcgc cgccgatcac ggcctccagc
     6241 gcctcggcgg acgcttccgc ggtatcccgg cgcgagagat ccatctgcac gccgcccagc
     6301 atcgcctgaa gcacctcccg gtcgaccaag cgtgatacac gttccatcac ggcctcggcg
     6361 agcaggtacg acttggcgag ctcggcgagc gtgtcgccgc cgatcgggtc gcgcccggcc
     6421 cccgggtgca gcgcggcgtc aacaagcgcc tggcgcagca tgaaggcatt gagctcgtgc
     6481 tcatccttga ggtagcgctc ctccttgcca agcctcgctt tgtagagcgg cggctgcgca
     6541 atgtagatgt ggccgcgctc tacgagctcg cgcatctgcc ggtagaagaa cgtgagcagc
     6601 agcgtgcgga tgtgcgagcc gtcgacgtcg gcgtccgtca tcacaatgat ccggtgatag
     6661 cggagcttgt ccgggttgta ctcctcggcg ccaatgccgc agccgagcgc ggtgatcagc
     6721 gtcgcgatct cggccgagga taggagcttt tcgaagcgcg cgcgctcgac gttgagaatc
     6781 ttgcccttga gcggcaggat cgcctggaac ttgcgatcgc gtccttgctt ggccgagccg
     6841 ccggcagagt cgccctcgac gagatagagc tcggacttgg ccgggtcgcg ctcttggcag
     6901 tcggcgagct tgccgggcat ccccatcgag tcgagcacgc ctttgcggcg ggtcatctcg
     6961 cgcgctttgc gtgcggcctc gcgcgcggcc gcggcctcga caatcttgac ggtaatcgtg
     7021 cgcgcgtcgt gcgggcgctc atcgagaaat tcgcgcagcc gctcggccac gatctcctcg
     7081 acgaccggcc gcacctcgga ggacaccaac ttctccttgg tctgcgagga gaacttcggc
     7141 tccggcactt tgaccgatag cacgcacgcg aggccttcgc gcatatcgtc gccggtggtc
     7201 tcgaccttgg ccttcttggc gatctcgtgc tcctcgatgt acttgttcaa caccctggtc
     7261 atcgcggcac gcaggccggt gaggtgggcg ccaccgtcct tttgcgggat gttgttggtg
     7321 aagcactgaa cgttctcctg gtaggagtcg ttccactgca tcgccacctc gaccgtgatg
     7381 ccgtccttga ccccgtggcc tagaaacacg ttggggtgca gcacgttctt cgcgcggttc
     7441 atgtactcga cgaatccctt cacgccgccg gagaaagcaa agttttcctc cttgcccgcg
     7501 cgctggtcga tgagcacgat cttcacgccg ttgttgagga acgagagctc gcgcagccgg
     7561 cgtgccagga tgtcatagtg gtaatcgacg ttgccgaagg tctccttcga agcgagaaaa
     7621 tgcacctcgg tgcctcgccg ctccgtcgcg ccagtcgccg caagtggtgc gaccgccacc
     7681 ccgttgcgga attcgatctg gtgcacccgg ccttcgcgcc agatcgtgag ccgcagccat
     7741 tccgacaacg cgttcaccac cgagaccccg acgccgtgca agccgccgga aaccttgtag
     7801 ctgttggagt cgaacttgcc gccggcgtgc agctcggtca tcacaatctc ggcagccgag
     7861 cgttttagcg tgtcatcgtc cttgacgccc gtcgggatgc cgcggccgtt atcaagcacc
     7921 gaaaccgaat tgtcggtgtg aatagtggcc acgatctcgt cgcaaaaccc cgcgagcgct
     7981 tcgtccaccg cgttgtcgac cacctcgaac accatgtggt gcaggccggt cccgtccgag
     8041 gtgtcgccga tgtacatccc ggggcgcttg cgcaccgcct ccaggccttt caggaccttg
     8101 atcgcgtccg agtcgtagtt cttttccgct tcccccacct agatcctcat cggcatgacg
     8161 acgtacttga agtccttttc cgcgccgtac gtgatgagcg cgctcgacgc cgcgtcgccg
     8221 aacgcgcact cgatctcgtc ccccgacacg ttgttcagca cgtcgagcag gtaattcacg
     8281 ttgaaaccga tatcgagccc gtcgcccgaa tacttcacgt cgagctcctc ctgcgcctcc
     8341 tcctgctcgg cgttggagga aacgatcttc agggaaccgt ccccgagtac ccagcgcacc
     8401 ccgcgaaact tctcgtttga caggatggcg gcgcgctgca gcgcctgacg cagcggctcg
     8461 cgagcggcgt gcaggcggtt cttgtggctc gtcgggatga cccgggtgta gtccggaaac
     8521 ttgccgtcga tcagctttga gacgagctcc acggcgccga agctgaacgc cgcttgcgtc
     8581 gccgcgatct cgatcctcac cggcgcgtcg ctgtccgcca gcagcttgcc gagctcgagc
     8641 acggtcttgc gcggcacgat cacttcctgg cgcggcagct gggcgccgag cttgagcgcc
     8701 gcataggcga ggcggtgtcc gtcggtcgcg ac
//
LOCUS       DASTPD010000187         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1116814, whole genome shotgun sequence.
ACCESSION   DASTPD010000187 DASTPD010000000
VERSION     DASTPD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116814"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1257)
                     /locus_tag="VFR57_06245"
     CDS             complement(91..1257)
                     /locus_tag="VFR57_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0259001.1"
                     /translation="MKRDTILTIDLPTTEGKLAAYPVREPRQFQKPAKPFTRKALAAA
                     HVVAEPLSTKDPWLEAAIDWDATIVYRRHLWSWGFGVAEAMDTAQRGMGLDWSSSLML
                     IEKSVREARTIAGAEVWSGAGTDHMAHTNLDGVIRAYEEQCGAIEKLGGRIILMASRA
                     LAACARSPEDYAEVYDRVLSQVSQPVIVHWLGEMFDPALEGYWGSKDHYAAMEVCLSV
                     LQRNAAKVDGIKISLLDKQKEVDMRRKLPAGLRMYTGDDFNFAELIQGDAQGYSDALL
                     GIFDAIAPAASEALSFLSEGRNSDYHAILGPTVPLSRHIFRAPTRFYKTGVVFMAWLN
                     GHQDHFVMVGGQQSARHVLHLAELFRLADAAGLLADPPLACARMKQLLAVHGCS"
     gene            complement(1254..2411)
                     /locus_tag="VFR57_06250"
     CDS             complement(1254..2411)
                     /locus_tag="VFR57_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0259002.1"
                     /translation="MSEKRIGILMHGITGRMGMNQHLIRSILEIRKQGGVALKDGTRV
                     MPDPLLVGRNAAKVEELARQHGVHRWTTDLEKALQSEDQIFFDAASTQLRPKLLKKAI
                     AAGKHVYCEKPVSTTLAEAKDLYAAAKKARVKHGVVQDKLWLPGMLKLRKLRDDGFFG
                     RILSVRGEFGYWVFEGDRQSGQPPAQRPSWNYRKQDGGGIILDMLCHWRYVLDNLFGE
                     VQAVSCLGATHIPHRWAEDGKRYKATADDAAYATFYLKGNIVAQFNSSWDVRVRRDDL
                     LTLQVDGTRGSAVAGLRKCLVQSYEKTPKPVWNPDIPQPINFFDGWEEVPDENYDNAF
                     KAQWELFLRHVAGDGPFRWNLLEGAKGVQLAELGLKSWKQRKWLDVPRLKP"
     gene            complement(2408..2860)
                     /locus_tag="VFR57_06255"
     CDS             complement(2408..2860)
                     /locus_tag="VFR57_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259003.1"
                     /translation="MSFRVLGTNHTSFTVSNLDRSLGFFRDCLGFELVSKAPRDPALV
                     SRITGVEGADTMIAFLKAPGHTLELIEYRAPASKGAVKARPCDTGFAHVAFNVDDAQA
                     AVAAVQRYGVRPISPPVTIDQGPNKGRKVVYVRDWDGVTIEFIEVATK"
     gene            complement(2857..>4137)
                     /locus_tag="VFR57_06260"
     CDS             complement(2857..>4137)
                     /locus_tag="VFR57_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0259004.1"
                     /translation="LRMPFRFGVVTLTAAPQAFVRARIVLENGKEAHGAAAELLAPKW
                     FDKNPALSNEQNFEQLRGSMRKARDEYLAGGVNAAYGHSRPTVGLVENFGPALLDRAV
                     LDGLCRALNISFYQAVRTNVPGIGFAGLDMPRFLAGLRPSGHIAARHTVGLLDPMTLR
                     DVKHPVNDGLPETLEQVIARYGHRWFKLKVGGDVKADVARLSAIAAVLERIDEPYHAS
                     LDGNEQYDSAQQVLELLEAMASEPRLKRLVASIVFIEQPIKRQNALESDVSALAARKP
                     IIIDESDDSLDAFPRARALGYRGVSSKTCKGIYKSLINAARCAAWGPGYFMSGEDLTI
                     QAGLALQQDLALVSVLGLTHVERNGHHYVNGMAELPQVEQERFLDSHPDLYERSHDAV
                     RLRIEKGMIRMVSLDCPGYASRALPEWKSMRRMP"
BASE COUNT          700 a         1336 c         1323 g          778 t
ORIGIN      
        1 ccaatacctg agcctgaaaa aaagcgtccc agactttgcg gaagtccggc tcgatgacat
       61 cggcttcggc gccgaacgaa gcgagctctc tcagctgcat ccgtgcaccg caagcagttg
      121 cttcatacgt gcgcatgcca acggtgggtc ggcaagcaga cccgcggcgt ccgcgagcct
      181 gaatagttcg gcgaggtgca gcacgtgacg cgccgactgc tgcccgccca ccataacgaa
      241 atgatcctgg tggccgttaa gccacgccat gaacaccacg ccggtcttgt agaaacgcgt
      301 cggcgcgcga aagatgtggc gtgagagtgg gacggtcggg ccgagaatcg cgtgatagtc
      361 gctgttcctt ccctcgctta gaaaagacag tgcctctgaa gcggcgggtg cgatggcatc
      421 gaaaatccca agcagcgcat ccgagtatcc ctgtgcgtcg ccctggatca gctcggcgaa
      481 attgaagtcg tcgccggtgt acatgcgcaa gccggcgggc agcttgcggc gcatgtcgac
      541 ttccttctgc ttgtcgagga gcgagatctt tatgccgtcg accttggcgg cgttccgttg
      601 gaggacggaa aggcagacct ccatcgcggc gtagtgatcc ttgctccccc agtaaccttc
      661 gagcgccggg tcgaacatct cgccaagcca gtggacgatc accggctggc tcacctgcga
      721 cagaacccgg tcgtacacct cggcgtagtc ctcgggcgag cgcgcgcaag cggcgagcgc
      781 gcggcttgcc atcaaaatga tgcgcccgcc gagcttctcg atcgcgccgc actgctcctc
      841 gtacgcgcgg atgacgccgt cgaggttcgt gtgggccatg tggtcggttc cggcccctga
      901 ccaaacttcc gcgccggcaa tggttctcgc ttccctgacg gatttttcga tcaacatcaa
      961 tgaagacgac caatcgagcc ccatgccgcg ctgcgcggtg tccatcgcct cggcgacgcc
     1021 gaagccccag gaccacaaat gccggcgata gacgattgtt gcgtcccagt cgatcgcggc
     1081 ttcgagccac gggtccttcg tcgaaagcgg ctcggcgacg acgtgagcgg cggccagcgc
     1141 cttcctcgtg aacggcttcg ccggcttttg gaactgcctg ggttcgcgca ccgggtaagc
     1201 cgctagcttg ccttcggtgg tcgggagatc aatagtcagt atcgtgtcgc gtttcatggc
     1261 ttgagcctcg gaacgtcgag ccactttctc tgcttccagc tcttgaggcc gagctcggcg
     1321 agctgcacgc ccttcgcgcc ttcgagcagg ttccagcgga acgggccgtc gcccgcaaca
     1381 tgccgcaaga acagctccca ttgcgccttg aatgcattgt cgtagttttc gtcggggact
     1441 tcttcccagc cgtcgaagaa attgatcggc tgcgggatgt cgggattcca gaccggcttg
     1501 ggggtcttct cgtagctctg caccaggcac ttgcgcaagc ctgcaacggc gctgcctctg
     1561 gtgccgtcga cctgcaacgt aaggaggtcg tcacgccgca cgcgcacgtc ccacgagctg
     1621 ttgaactggg cgacgatatt tcccttcaaa tagaacgtcg cgtaagcggc gtcgtccgcg
     1681 gtcgccttgt agcgcttgcc gtcctccgcc caccggtgcg gaatgtgggt cgccccgagg
     1741 cagctcaccg cctgcacctc gccgaagagg ttgtcgagca cgtagcgcca gtggcagagc
     1801 atgtcgagga tgatgccccc gccgtcctgc ttgcggtagt tccacgacgg gcgctgagcc
     1861 ggcggttgac cgctctgccg gtcgccctcg aacacccaat agccgaactc gccccgcacc
     1921 gagaggatgc ggccgaagaa gccgtcgtcg cgcagcttgc gcagcttcag catccccggc
     1981 agccagagct tgtcctgaac cacgccgtgc ttgacgcgcg ccttcttcgc agccgcgtag
     2041 aggtccttcg cttcggcgag cgtggtcgaa accggcttct cgcagtagac gtgcttgccc
     2101 gcggcgatcg ctttcttcag gagcttcggg cgaagctgcg tggacgcggc gtcgaagaag
     2161 atctggtctt ctgattgaag agctttttcc agatcggtcg tccagcgatg cacgccgtgc
     2221 tggcgcgcga gctcctcgac cttggcggcg ttgcgcccga cgaggagcgg gtcgggcatg
     2281 acgcgcgtgc cgtccttgag cgccacgccg ccctgcttgc ggatctcgag aatcgagcgg
     2341 atcagatgct ggttcatgcc catgcggccg gtgatgccgt gcatgaggat gccgattcgc
     2401 ttttcgctca ttttgtggcc acctcgatga actcgatcgt cactccgtcc cagtcgcgca
     2461 cgtacaccac cttgcgcccc ttgttcggtc cctggtcgat ggtgacgggc ggcgagatcg
     2521 gtctcacgcc gtagcgctgc accgccgcga cggccgcttg ggcgtcgtcg acgttgaacg
     2581 cgacgtgcgc gaacccggtg tcgcacggcc gcgccttgac cgcgcccttg ctcgccggtg
     2641 cgcggtactc gatcagctcg agcgtgtggc cgggcgcctt gaggaacgcg atcatcgtgt
     2701 ccgcaccctc gacgccggta atgcgcgaca cgagcgccgg atcgcgcggc gccttggaga
     2761 cgagctcgaa gccgaggcag tcgcggaaga acccgagcga gcggtcgagg ttcgagacgg
     2821 tgaagctggt gtggttggta cccagtaccc ggaagctcaa ggcatcctcc tcatcgattt
     2881 ccattccggc agggcgcggc tggcgtaacc cggacagtcg agcgacacca tgcgaatcat
     2941 tcctttctcg atgcgcaggc gcaccgcatc gtggctgcgc tcgtagaggt cagggtggga
     3001 atcgaggaaa cgctcctgct cgacttgggg cagttcggcc attccgttga catagtgatg
     3061 gccgtttcgc tcgacgtgcg tgaggccgag gaccgagacg agcgcaagat cctgctgtag
     3121 cgcgagcccg gcctgaatcg taaggtcctc gccgctcatg aagtaacccg gtccccacgc
     3181 ggcgcagcgg gcggcgttga tcagggactt gtagatgccc ttgcatgtct tcgacgacac
     3241 gccgcgatag cccagcgccc tcgctcgcgg aaacgcatcg agcgagtcgt cggactcgtc
     3301 gatgatgatc ggcttgcgtg cggcgagcgc ggaaacgtcc gactcgagcg cgttctgccg
     3361 cttgatcggc tgctcgatga aaacgatgct cgcgacgagg cgcttcaggc gcggctcgga
     3421 cgccatcgct tcgagcagct cgagcacctg ttgcgcactg tcgtattgct cgttgccgtc
     3481 gagcgaagcg tggtagggct cgtcgatccg ctcgaggacc gccgcgatcg cgctcaagcg
     3541 cgcgacgtcg gccttgacgt cgccgccgac cttgagcttg aaccagcggt ggccgtagcg
     3601 cgcaatcacc tgctccagcg tctcgggcaa tccgtcgttc accgggtgtt tgacgtcgcg
     3661 tagcgtcatc gggtcgagca gccctacggt gtgccgggcg gcgatgtggc cggaaggtcg
     3721 caggccggcg aggaagcgcg gcatatcgag ccccgcaaaa ccaattcccg gcacgttggt
     3781 cctgaccgcc tggtagaagg aaatgttgag cgctcggcag agcccgtcga gtaccgcccg
     3841 gtcgaggagc gccgggccga agttctccac cagcccgacc gtcggccgcg aatgcccata
     3901 cgcggcgttc actcccccag cgagatattc gtctcttgcc ttgcgcatcg agccgcgcag
     3961 ctgctcgaag ttctgctcgt tactgagcgc cgggttcttg tcgaaccact ttggcgcaag
     4021 caactcggcg gcggcaccgt gcgcttcctt tccattctca agaacaattc gagcgcggac
     4081 gaacgcctgg ggggcagcgg tcagtgtgac cacgccgaaa cgaaacggca tgcgcag
//
LOCUS       DASTPD010000188         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_709156, whole genome shotgun sequence.
ACCESSION   DASTPD010000188 DASTPD010000000
VERSION     DASTPD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709156"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1439
                     /locus_tag="VFR57_06265"
     CDS             180..1439
                     /locus_tag="VFR57_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0259005.1"
                     /translation="MKSWVEKTIAAVGRRTEIPFAVRYADGTEARSRDAVPAFTLVFR
                     NRRAYWRIAAFGHVGLLEAHFDGELDIEGSLSKALAAGMEGGFDGGDGPLVWLRNRWH
                     ELTHSNAVRSRARANAEAHYALPPEFYKFWLDDPLMLYTCAYWTDGTRTLEEAQRNKC
                     DHVARKLLLKPGEEVIDVGSGFGGFMFHAHERYGVKVTAVNTTGSQAEHARREIERKR
                     LGNALSMQMHEDFRDAGQRQFDKVASIGCLEHAGRDQLAELIRAHAGYLKKGGIGLIH
                     FIGHVGRFDTDFFIRKYVFPGGWIPSLADVIVEMENAGLEILDVENLRRHYALTLDVW
                     AERFDRSWDKIRALDPKRFDERFRRIWRVYLYGCAEMFRAPNGRTHLFQVVFSKGNVT
                     TSSYPMTREFLYEQVKAEAAPRRVGHG"
     gene            1432..2772
                     /locus_tag="VFR57_06270"
     CDS             1432..2772
                     /locus_tag="VFR57_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0259006.1"
                     /translation="MVDAAAAHAAKRESLRESLRQGAGPVALAKETSNLFRDRGAGER
                     RRLDVRGFNQVLRISGGWVEAEGMIPYDDLTRECLARAVMPAVVPQLKTITLGGAVAG
                     VGIEASSHRQGLVHDTMLELDVLLGDGRVVSCTPDNAHADLFFGFPNSYGTLGYALRV
                     KARTVPVKPYVHLSHLPFKNGRDYFSQLEEHLRAGKADFVDGTIFSPERLFITLGRFV
                     DSAPFTSDYTYKNIYYRSIAEKREDYLTAHDYIWRWDTDWFWCSKNVFAQNAFIRRFV
                     YGKNRLGSRTYTKIMRWNSRVGATKKLERILGLHSESVIQDVDIPISRAAEFLDFYSR
                     EIGLWPQWICPIGPQSHSGRFVLYPVQNTWYVNFGFWDLKRTREAHAPGHFNRMIEDK
                     VSELGGIKSLYSDSFFSEADFHARYGGAAYDALKAKYDPGGAFPRLYDKCVLRA"
     gene            complement(2846..3238)
                     /locus_tag="VFR57_06275"
     CDS             complement(2846..3238)
                     /locus_tag="VFR57_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU0259007.1"
                     /translation="MALTEKQIQELQGAIEQRRTSLVAELRDDVERVRRDRFEDLAGA
                     APDPGDESVATLIADLGHADMERDLSELRALEAARARLADGSYGVCAECGGDIGVERL
                     RANPAAVRCVHCQRVHEKTFAGPSTSSL"
     gene            complement(3265..4158)
                     /locus_tag="VFR57_06280"
     CDS             complement(3265..4158)
                     /locus_tag="VFR57_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU0259008.1"
                     /translation="MKYKDYYKILGVERGASDEDIKKAYRKLARRYHPDVSKEANAKE
                     RFQEVSEAYETLRDKEKRAAYDSLGSFRPGQDFRPPPDWFERFGSGRNEDLHGVDLGE
                     LFESMGLFGRAGRRRSRGVFPGEDYEVPVRLTLEEAFRGTERTVQLDNRSFTARIPRG
                     ATDGQRLRLRGKGGLGVNGGPPGDLYLQIALEPHPLYRASGHDLEIEVPLAPWEAALG
                     APVEVPTLEGRVTMKVPAGSRAGQKLRLSSKGLPKPGGGAGDLYAVLTIAVPATLTER
                     EKALYEELRQASRFNPRLRFG"
     gene            4415..>4559
                     /locus_tag="VFR57_06285"
     CDS             4415..>4559
                     /locus_tag="VFR57_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU0259009.1"
                     /translation="MHFDRELDARGLNCPLPILRAKKALNEMHSGQVLKIVATDPGSV
                     KDFQ"
BASE COUNT          755 a         1533 c         1481 g          790 t
ORIGIN      
        1 gagatgagct tgcgcacttc ttcgggggac ggttgcggat tgctgactgc cttcggcgtt
       61 ctcttggccg gcgcagcgcg ctctttcctg gctgccatgg cgaggctcct tgcgttggtg
      121 cccgccgagg ctagcagaag tggtgccccg gattccaccc gattgtaaaa ttcccccccg
      181 tgaagtcctg ggtcgagaaa acgatcgccg cggtgggcag gcgcaccgag atcccgttcg
      241 ctgtccggta tgccgacgga accgaggccc ggtcgcggga cgcggtgccg gcgttcacgc
      301 tggtgtttcg caaccgccgt gcctactggc gcatcgcggc tttcggccac gtcgggctgc
      361 tggaagccca cttcgacggc gagctcgaca tcgaaggcag cctgtccaag gcgctcgctg
      421 ccgggatgga aggcggtttc gacggcggtg acgggccgct ggtctggctg cgcaaccgct
      481 ggcacgagct cacgcattcg aacgccgtgc gctcgcgggc gcgcgccaac gccgaggcgc
      541 actacgcgct gccgccggag ttctacaagt tctggctcga cgacccgctc atgctctaca
      601 cctgcgcgta ctggaccgac ggcacgcgca cgctcgagga ggcgcagcgc aacaagtgcg
      661 accacgtggc gcgcaagctg ctcctcaagc cgggcgagga ggtgatcgac gtcggcagcg
      721 gcttcggcgg cttcatgttc cacgctcacg agcgctacgg cgtgaaggtg accgcggtca
      781 acaccaccgg gtcgcaggcc gagcacgcgc gccgcgagat cgagcgcaag cgcctcggca
      841 acgcgctctc catgcagatg cacgaggact tccgcgacgc cgggcagaga cagttcgaca
      901 aggtggcgtc gatcggctgc ctggagcacg ccgggcgcga tcagcttgca gagctcattc
      961 gcgcgcacgc gggctacctg aagaagggtg gcatcggcct catccacttc atcggtcatg
     1021 tcgggcgctt cgacaccgac ttcttcatcc gcaagtacgt gttccccggg ggctggattc
     1081 cgagcctcgc cgatgtgatc gtggagatgg agaacgccgg cctggaaatt ctcgacgtcg
     1141 agaacctgcg ccggcactac gctcttacgc tcgacgtgtg ggccgagcgc ttcgaccgca
     1201 gctgggacaa gatccgcgcg ctcgacccga aacgcttcga cgagcgcttc cggcgcatct
     1261 ggcgcgtgta cctctacggc tgcgccgaga tgtttcgcgc gccgaacggc cgcacgcacc
     1321 tgttccaggt ggtgttctcc aagggaaacg tcaccacctc gagctacccg atgacgcgcg
     1381 agttcctgta cgagcaagtg aaggccgaag ccgccccgcg ccgcgtcggg catggttgat
     1441 gcggctgcgg cgcatgccgc caagcgcgaa tctctccgcg agtccctgag gcagggcgcc
     1501 ggaccggtcg ccctggcgaa ggagacatcc aaccttttcc gcgaccgcgg ggccggcgag
     1561 cggcgccggc tggacgtcag gggcttcaat caggtacttc ggatctcggg cggttgggtc
     1621 gaagccgaag gcatgattcc ctacgacgac ctgacccgcg aatgcctcgc gcgcgccgtg
     1681 atgcccgccg tggtgccgca gctcaagacc atcacgctcg gcggcgcggt tgcgggggtc
     1741 ggcatcgagg cttcgtcgca ccgccaaggc ctggtgcacg acactatgct cgagctcgac
     1801 gtgctgctcg gcgacggccg cgtcgtgagc tgcacgccgg acaacgcgca cgccgacctc
     1861 ttctttggct ttcccaactc gtacggcacg cttggctatg cgctgcgcgt aaaggcgagg
     1921 accgtgccgg tgaaaccgta tgtgcacctg tcgcacttgc cgttcaagaa tggacgagat
     1981 tatttctcgc aactggaaga gcatctgcgc gcgggaaagg ccgatttcgt cgatggcacg
     2041 atcttttcgc ccgagcggct tttcatcacg ctcggccgtt ttgtcgattc ggcgccgttt
     2101 accagcgatt acacgtacaa gaacatctac taccgctcga tcgccgagaa gcgcgaggac
     2161 tacctcaccg cgcacgatta catctggcgc tgggataccg actggttctg gtgctcgaag
     2221 aacgttttcg cgcagaacgc tttcatcagg cgattcgtct acggcaagaa ccggctgggc
     2281 tcgcgcacct acacgaagat catgcgctgg aacagccgcg tcggcgcgac gaagaagctc
     2341 gagcgcatcc tcgggctgca ttcggaatcg gtgatccagg acgtcgacat tccgatctcg
     2401 cgcgcggccg agtttctcga cttctactcg cgggagatcg gcttgtggcc gcagtggatc
     2461 tgtccgatcg ggcctcaatc ccactccggc cgattcgtgc tctaccccgt gcagaacacc
     2521 tggtacgtca acttcggctt ctgggacctg aagcgcacgc gcgaggcgca cgcgccgggg
     2581 cacttcaacc gcatgatcga ggacaaggtg agcgagctcg gcggcatcaa gtcgctgtac
     2641 tccgacagtt ttttctccga ggccgatttc cacgcccgct acggcggcgc cgcctacgac
     2701 gcgctcaagg cgaaatacga cccgggagga gcgttccctc gtctatacga caaatgcgtc
     2761 ttacgggcat gaggaattcg ggggcagggt cgcaaattca gttttcacgt acgcaaaaac
     2821 gaattgcgac cctgcccccg aattcctaca ggctcgaagt gctcggcccg gcaaacgtct
     2881 tctcgtggac gcgctggcag tggacgcagc gcaccgcggc cgggttggcg cgcagccgct
     2941 cgacgccgat gtcgccgccg cattccgcgc agaccccgta gctgccgtcg gcgaggcgcg
     3001 cgcgagccgc ctcgagcgcg cgcagctccg agaggtcgcg ctccatgtcg gcgtggccga
     3061 ggtcggcgat gagcgtcgcc accgactcgt cgcccgggtc gggcgccgca ccggcgaggt
     3121 cctcgaagcg gtcgcggcgt acccgctcga cgtcgtcacg tagctcggct accagcgagg
     3181 tgcgccgctg ctcgatcgcg ccctgcagtt cctgaatctg tttttccgta agggccatgc
     3241 gcccccgact cgcaaagagc gtgcctagcc gaagcgcagg cgtggattga aacgcgaagc
     3301 ctggcgcagc tcctcgtaca gcgccttctc tcgctcggtg agcgtcgctg gcactgcgat
     3361 cgtgagaacg gcgtagaggt cgccggcgcc gccgccgggc ttggggaggc ccttgctgct
     3421 gaggcgcaac ttctggccgg cgcgcgagcc cgccggcacc ttcatcgtga cgcgcccctc
     3481 gagcgtcggc acctccaccg gcgcgccaag cgcggcctcc cagggcgcga gcggcacttc
     3541 gatctcgagg tcgtgcccgc tagcgcgata cagcggatgc ggctcgagcg cgatctggag
     3601 gtagagatcc cccggcggtc cgccgttcac cccgaggccg cccttgcctc gcagccgcag
     3661 ccgctggcca tcggtcgcgc cgcgcggaat gcgcgcggtg aagctacggt tgtcgagctg
     3721 caccgtgcgc tcggtgcccc ggaaggcctc ttcgagcgta agccgcaccg gcacctcgta
     3781 gtcctcgccc ggaaaaacgc cgcgcgagcg gcgccgcccc gcgcggccga acagccccat
     3841 cgattcgaag agctcgccca ggtctacgcc atgcaggtcc tcgttgcggc ccgagccgaa
     3901 gcgttcgaac cagtcgggcg gcgggcgaaa atcctggcca ggacgaaagc ttcccaggct
     3961 gtcgtacgcc gcgcgctttt ctttgtcgcg cagggtctcg taggcttccg agacctcctg
     4021 gaagcgctcc ttggcgttcg cctctttcga cacatcgggg tgatagcggc gcgcgagctt
     4081 gcggtaggcc ttcttgatgt cctcgtcgga ggcgccccgc tccacgccga ggatcttgta
     4141 gtagtccttg tatttcattc ccccaatatt gggggtggtc gagtcagcgc gcaacgtgcc
     4201 ggcggatggc ctcccggaac tccggccggc gcaacagccc gcgaggatgc acggtcggat
     4261 gcgtagcgcc cgccacgaca tcgacctcga gccgccagca gagcaaggac gattgcctat
     4321 gcacgccgct ccatggcatc cgctctctct atgaaaggat atcgatctcc cgatggcgag
     4381 ccgataccct ccgtgtaata cttgcggcgc gatcatgcac ttcgacagag aactcgacgc
     4441 ccgcgggctg aactgcccgt tgccgatcct gcgcgccaag aaggcgttga acgagatgca
     4501 ctcgggtcag gtgctcaaaa tcgtggccac cgatccgggc tcggtgaagg acttccagg
//
LOCUS       DASTPD010000189         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1526021, whole genome shotgun sequence.
ACCESSION   DASTPD010000189 DASTPD010000000
VERSION     DASTPD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526021"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFR57_06290"
     CDS             <1..413
                     /locus_tag="VFR57_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259010.1"
                     /translation="ASDGAVEHAAHQAHAHDFIMRLPFGYDTELSEAGAGLSGGERQR
                     LSIARAFLKDAPILILDEPTAALDAVSEREVFRALEALRAGRTTLVIAHRLSTVRAAD
                     RILVLENGRIVAEGTHQSLLTSSEHYRRLTGDFT"
     gene            complement(436..675)
                     /locus_tag="VFR57_06295"
     CDS             complement(436..675)
                     /locus_tag="VFR57_06295"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU0259011.1"
                     /translation="MSDELRRGGDNRPRRDESSSTVTSRLRKASANQAVPIDPNLVAH
                     RAYDRYQRRGGEHGRNLQDWLEAERELIAAQFEET"
     gene            complement(729..986)
                     /locus_tag="VFR57_06300"
     CDS             complement(729..986)
                     /locus_tag="VFR57_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259012.1"
                     /translation="MFNLTVRGLTGRNLPLFFISRQIIADGLLVLRRGHRECPLLETD
                     YRPMERRRRRLRVKMGIGDGNAVRWTFSEVLGDSSALLNPT"
     gene            1232..2371
                     /locus_tag="VFR57_06305"
     CDS             1232..2371
                     /locus_tag="VFR57_06305"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEU0259013.1"
                     /translation="MAMRLRIRDTIEELVQQELDAALGAVKSARVGETRQGYRHGTRE
                     RTLTTSLGPTTIAMPRARVRGAEGTSEEWRSETVRRYQRRTTRVDEAILGVYLAGGNT
                     RRIKGALAPLLRGGPLSKDAVSRLVGRLREDFETWRARDLADEDVRYVFMDGWYPKVR
                     IGGRRERVPVLVTLGVRANGDRVVLDMRLVGEESAASWTEVVASLVARHLARPVLAVI
                     DGNPGLSSALRSHWPGIAIQRCTAHKLRNLQAKAPARLREELTEDYRRMIYADTVSAV
                     EHERARFTKKWQLRCPAVVESLKEAGDELFTFLRFPPSQWKALRTTNALERINEEFRR
                     RTKTQASLPGQDAVLLLLFGLLRSGQVKLRALVGYQDMEQVKKAA"
BASE COUNT          448 a          825 c          951 g          434 t
ORIGIN      
        1 atgccagcga cggggccgtc gagcacgcgg cgcaccaggc gcacgcacat gacttcatca
       61 tgcgcctgcc gttcggatac gacaccgagc tgtcggaagc gggcgccggt ctctccggcg
      121 gcgagcgcca gcggctgagc atcgcccgtg cgttcctcaa ggacgcgccg attctgattc
      181 tcgatgaacc caccgccgcg ctggacgccg tttcggagcg cgaggtgttc cgcgcgctcg
      241 aagcgctgcg cgcggggcgc accacgctcg tgatcgccca ccggctgtcg accgtccggg
      301 cggccgatcg catcctggtc ctggagaacg gccgaatcgt cgcggagggg actcaccagt
      361 cgctgctcac gtcgagcgag cactatcggc ggctcacggg ggacttcacg taggtccgcg
      421 atccggggtc cgggatcacg tctcttcgaa ctgcgcggca atcagctcgc gctcggcttc
      481 gagccagtcc tgcagattgc ggccgtgctc gccgccgcgc cgctgatatc gatcgtacgc
      541 tcgatgcgcc accaggttgg gatcgatcgg gacggcctgg ttcgccgaag ccttgcgaag
      601 acggctggtg accgtcgagg agctctcatc acggcgcggg cggttgtcac cgccgcgcct
      661 cagctcgtcg gacatgcaga tgtctcctgc ggatgcgtgc gtcggtctgc cggcgtgccg
      721 cgcgcaaatc acgtgggatt caacagtgcg gatgagtcgc cgaggacctc gctgaacgtc
      781 catctcaccg cgttaccgtc accgatgccc atcttcacgc ggagccggcg tcgccgccgt
      841 tccatcggcc ggtagtcggt ttcgaggagc gggcactcgc gatggccccg ccggagcacc
      901 aggagaccgt ccgcgatgat ctggcggctg atgaagaaca ggggcaggtt ccggccggtg
      961 aggccgcgga cggtgaggtt gaacaatcgt gctccttcag gggacgcgtc gatgacgcgg
     1021 ccgtagggat cggtatcgac ccacggcccg gcgaggtagt gtcttgcgtg tggtggaaaa
     1081 gtagaaatcg cgtcatcatc cggcgcagtt caacccaaga gctgcgcgcc cgcttggcgg
     1141 cgggtgccgg gaggatgacg cgatggagca ctgtaccaag aagccaggcg aggagaaaga
     1201 gattccggcg ttcggcccgg acagcatcga gatggcgatg cggctgcgga tccgcgacac
     1261 gatcgaagag ctggtgcagc aggagctcga cgccgcactg ggggccgtca agtcggcgcg
     1321 cgtgggcgag acgcggcagg gctatcggca tgggacgcgg gagcggacgc tgacgacgag
     1381 cctggggccg acgacgatcg cgatgccgcg ggcgcgagtg cgtggggccg aggggacgag
     1441 cgaggagtgg cgcagcgaga cggtgcgccg gtatcagcgc cggaccacac gggtagatga
     1501 agcgattctg ggcgtctacc tcgcgggcgg caacacgcgg cgaatcaaag gcgctttggc
     1561 gccgctgctg cgtggtggtc cgctgtcgaa ggatgcggtc tcgcggctgg tcggccgcct
     1621 gcgcgaggac ttcgagacgt ggcgcgcccg tgatctcgcc gatgaggatg tccgctacgt
     1681 gttcatggac ggctggtatc cgaaggtgcg catcggcggc cgccgcgagc gagtgcccgt
     1741 gttggtgacc ttgggcgtgc gggcgaatgg ggaccgggtg gtgctcgaca tgcggttggt
     1801 cggcgaggaa agtgccgcga gctggaccga agtggtcgcg agcctcgtcg ctcgccactt
     1861 ggcgcggccg gttctggccg tgatcgacgg gaatccgggc ctgagcagcg cgctgcggtc
     1921 gcattggccc ggtatcgcga ttcaacgctg tactgcgcac aagctgcgga acctgcaggc
     1981 gaaagcgccg gcgcggctcc gcgaagagct cacggaagac taccgccgga tgatctatgc
     2041 cgacacggtc tcggccgtcg aacacgagcg cgcccgcttc acgaagaaat ggcagctgcg
     2101 ctgcccggcg gtggtcgaga gcctgaagga agccggcgat gagttgttca ccttcctgcg
     2161 gtttcctccg tcgcagtgga aagccttgcg caccacgaac gcgctcgagc ggattaacga
     2221 ggaatttcgt cgacgcacga agacgcaggc gagcttgccg ggacaggacg cagtcctcct
     2281 gctgctcttc ggcttgctgc gcagcggaca ggtgaagctg agagcgctcg ttggatatca
     2341 ggatatggag caggtgaaaa aggcggcgta ggtcacatca ggacgccgga tcgtgacgcg
     2401 ctgaactttt ccaccacttg acagacacga cctacctcta ccgttcggcg cccctgtatg
     2461 cattgacgtc ctcccaactc agagctacgc tgcctcgctt gcaataggat tgcgcgcact
     2521 cgcgcgcacc cttccaaggg aggacagatg cgcgtccagc gagtcctggg cagagacctc
     2581 tggctggtcc tgggtctgtc actgctgctc gccaccagcg cgagcgcgca ggtcgacacg
     2641 ggatcgatcc tcgggacg
//
LOCUS       DASTPD010000190         4365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_504864, whole genome shotgun sequence.
ACCESSION   DASTPD010000190 DASTPD010000000
VERSION     DASTPD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504864"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFR57_06310"
     CDS             complement(<1..519)
                     /locus_tag="VFR57_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259014.1"
                     /translation="MKKLALAVAAAFAVTAYTGAAFTQEKTVRITGFGAKSGVVRSFG
                     INSEAAMLAAADMINKQGGVKLADGSKAKMVVDFLDDRCTAEEGISVLRRIYGTDALV
                     AVGPTCSNVAEPLFGILQKKVGDSGDSGLQMPVFTDVAIKVGLAKISEWAFRNVPNET
                     IMYNSLFYWLKTK"
     gene            683..2296
                     /locus_tag="VFR57_06315"
     CDS             683..2296
                     /locus_tag="VFR57_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0259015.1"
                     /translation="MWAALQPGPANYTALSPIVFLPRAAEIYPERTAILHGGTRFTYK
                     ELFERARRLASALQKAGVKRGDVVSAMLPNIPAMLEVHYGVPMLGAVLNTINTRLDAD
                     TVAYILAHAEAKVFITDRVFAAVTGPALSKLRKKPLVVDVDDPQYTGPGELLGKIEYE
                     KFIAAGNADFPWSLPESESDPIALNYTSGTTGRPKGVVYHHRGTFVEACGNVMAWAMP
                     ANPVYLWTLPMFHCNGWCFPWSVTAMAGTHVCLRAVEPKRIFELIVEHGVTHMCGAPT
                     VLGMLIAAPEEERRRFPHPVHIQTGGSPPPAKVIKAMEELGFQVLHIYGMTELQGPST
                     FCASQDEWGNLSLEERANHMARQGVRYPVVEGHIVADPKTCKPVPKDGKSIGEILVRG
                     NTVMLGYLKDGKATAEAFEGGWMHTGDLAVWHPSNYVEIKDRKKDIIISGGENISSVE
                     VEIALYKHPATQLAAVVARPDEKWGETPCAFVQLKPGASATEQDYLDWCRENLARFKI
                     PKKVVFGPVPTTATGKIQKYVLRERAKAL"
     gene            complement(2307..3215)
                     /locus_tag="VFR57_06320"
     CDS             complement(2307..3215)
                     /locus_tag="VFR57_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0259016.1"
                     /translation="MFTASTELERYAWLSIAAALATITLKTLAWWLTGSVSLLSDALE
                     SIVNLAAALLALTMLRVAASPPDENHPYGFSKAEYFAAGIEGALIVLAAGGILASAIP
                     RLVEPQPIQAPLLGLALSAAASGINLGVGMLLIRVGRREHSIALEADGHHLMTDVWTS
                     AGVIVGVALVFLTGWLRLDPLVALAVAVHIVWIGLRLMRRSWKGLLDAAISIEDTGEV
                     TRLFTEYSKRYGVSFHALRTRQAGARRFISFHFLVPDAWTVAQAHRLSEEVEARIRSM
                     VPNAAVFTHIEPISDPSSYDDQELDR"
     gene            complement(3190..3663)
                     /locus_tag="VFR57_06325"
     CDS             complement(3190..3663)
                     /locus_tag="VFR57_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259017.1"
                     /translation="MSRGLLLTLTEPPPAMEEEFNAWYDSEHLAERLAISGFRSARRW
                     VRGATYLATYELDSPEVLQSRPYLERFRNQTPWSRRCLGRCLTFKRWACTQIEPGDAD
                     PHGAAAALAFTLKPERFSAVLQSRQFAAESGEAIFLYELSDARQHSEDVYRLYGT"
     gene            complement(3674..4318)
                     /locus_tag="VFR57_06330"
     CDS             complement(3674..4318)
                     /locus_tag="VFR57_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259018.1"
                     /translation="MLEVRGLNAGYGRAQVLFDVDLDVGQGEVVALLGRNGAGKSTSL
                     KAIMGLVPRLSGEIVFDAKPIRNLEPYEIARLGLGYVPEDRRIFTDLTVIENLEVARQ
                     APRGAAPPWTEERLFALFPALARLRDRRGARMSGGEQQMLCIARTLAGNPSAILLDEP
                     SEGLAPLVVAQVAQAIREIKRAGVTVLLAEQSRHFTDRVADRAYVLEKGVIGRG"
BASE COUNT          807 a         1419 c         1434 g          705 t
ORIGIN      
        1 tttcgtcttc agccagtaga agagcgagtt gtacatgatg gtctcgttgg gcacgttgcg
       61 gaacgcccac tccgagatct tcgcgagccc caccttgatc gcgacgtcgg tgaacaccgg
      121 catctgcagc ccggagtcac cggagtcgcc gaccttcttc tgcaggatgc cgaagagcgg
      181 ctccgccacg ttggaacagg tcggtccgac cgcgaccagc gcgtcggtgc cgtagatgcg
      241 gcgcaggacc gagatgcctt cctctgccgt gcagcggtcg tcgaggaaat cgaccaccat
      301 cttcgccttg gacccgtcgg cgagcttcac gccgccctgc ttgttgatca tgtcggccgc
      361 ggcgagcatc gccgcctcgc tgttgatccc gaacgagcgc accacgccgg atttcgcacc
      421 gaacccggta atacgcaccg tcttttcttg cgtgaacgcc gcgccggtat acgcggtgac
      481 cgcaaaagcg gcggcgaccg ccaaagcaag cttcttcatg agactcctcc tcgtgtggac
      541 tgtctggtag atttccgcag acccgaagcg agtgtagaac gaggagggag tcatggcaaa
      601 gcgagcgagc cgacacgccc gtcgcgcccg tttgagaaaa aaggttgctg cagcgcggaa
      661 gtcgccgtcg aaagctgttt ccatgtgggc ggcgctgcag ccgggtccgg ccaactacac
      721 tgcgctttcg ccgatcgtct tcctgccccg cgcagcggag atctaccccg agcggaccgc
      781 catcctccat ggcggcaccc gtttcactta caaggaactt ttcgagcggg cgcggcgcct
      841 tgcctctgcg ttgcagaaag ccggagtgaa gcggggcgac gtcgtctcgg caatgctgcc
      901 gaacattccg gccatgctcg aggtgcacta tggcgtgccg atgctggggg cggtgctcaa
      961 caccattaac acgcgcctcg acgccgacac ggtcgcctac atactcgcgc acgccgaggc
     1021 caaagtcttc attaccgacc gcgtattcgc ggccgtgacc gggccggcgc tgtcaaagct
     1081 caggaaaaag ccgctggtgg tcgatgtcga cgacccgcaa tacaccggcc ctggcgagct
     1141 gctcggcaaa atcgagtatg aaaaattcat tgcggcggga aatgcggact ttccctggtc
     1201 gcttcccgag tccgaatctg atccgatagc gctcaattac acctccggaa ccacgggccg
     1261 gccgaaaggc gtggtctatc accaccgcgg cacgttcgtg gaggcctgcg gcaacgtgat
     1321 ggcgtgggcc atgccagcga atcccgtgta cctctggacg ttacccatgt tccactgcaa
     1381 cggctggtgc ttcccgtggt cggtcaccgc catggccgga acccatgtct gcctgcgcgc
     1441 ggtcgagccc aagcgcatct tcgagctcat cgtcgagcac ggcgtcactc acatgtgcgg
     1501 agcgcccacc gtgctcggca tgctgatcgc cgcgccggaa gaggagcggc gcaggttccc
     1561 acacccggtg cacatccaga ccggcggctc gccgccgccg gcaaaggtca tcaaggcgat
     1621 ggaggagctc ggcttccagg tgctgcacat ctacggcatg accgagctcc aggggccttc
     1681 caccttctgt gcatctcagg atgagtgggg caatctttcc ctggaagaaa gagcgaacca
     1741 tatggcccgt caaggcgttc gctacccggt tgtcgaaggc cacatcgtcg ccgatccgaa
     1801 gacctgcaag ccggtgccga aggacggaaa gtcgatcggc gagatcctgg tgcgcggcaa
     1861 taccgtaatg ctcggctacc tgaaggacgg caaagccacc gcggaggcgt tcgagggagg
     1921 ctggatgcac accggcgacc tcgcggtgtg gcacccgagc aactatgtcg agatcaaaga
     1981 ccgaaagaag gacatcatca tttcgggagg cgagaatatc tcctcggtcg aagtggagat
     2041 cgcgctttac aagcacccgg caacgcagct cgccgccgtg gtggcgcggc cggacgagaa
     2101 gtggggcgag acgccgtgcg ccttcgtgca gctcaagccg ggtgccagcg ccaccgagca
     2161 ggactacctc gactggtgcc gggagaacct ggcgcgcttc aagatcccga agaaggtggt
     2221 gttcggccca gtgccgacga cggccaccgg caagatccag aagtacgtcc tgcgcgaacg
     2281 cgccaaggcg ctctgactaa accgctctag cggtccagct cctggtcgtc gtaggaggag
     2341 ggatcggaaa tcggctcgat gtgggtgaac acggcggcgt tgggcaccat cgagcggatg
     2401 cgcgcctcga cctcctccga gagccggtgc gcctgcgcca cggtccaggc atcgggcacc
     2461 aggaagtgaa acgagatgaa gcggcgcgcg ccggcctggc gcgtgcgcaa ggcatggaag
     2521 ctcacgccat agcgcttgga gtactcggtg aacagcctgg tcacttcgcc ggtgtcctcg
     2581 atcgagatcg cggcgtcgag aagtcctttc caggagcggc gcatcagccg cagtccgatc
     2641 cagacgatgt gcaccgccac ggcgagcgca accagcgggt cgagccgcag ccagccggtc
     2701 aggaatacga gcgccacgcc gacgatcacg ccggccgagg tccagacgtc ggtcattagg
     2761 tggtggccgt cggcctcgag cgcgatgctg tgctcccggc gtccgacccg gatcagcagc
     2821 atgccgacgc ccagattgat gcccgacgcc gcagcgctga gcgcaaggcc caggagcggc
     2881 gcctggatcg gctgcggctc gaccaggcgg gggatggcac tcgcgagaat cccgcctgcg
     2941 gcgagcacga tgagcgcgcc ttcgatgccg gcggcgaaat actccgcctt ggagaagccg
     3001 tacggatggt tctcgtcggg aggcgatgcc gcaacgcgca gcatggtaag cgcaagcagc
     3061 gcggcggcca ggttcacgat cgattcgagc gcgtcggaaa gcaggctgac ggagccggtg
     3121 agccaccagg cgagcgtctt cagcgtgatc gtcgcgagcg ccgccgcgat cgacagccac
     3181 gcgtagcgct caagttccgt agaggcggta aacatcctcg ctgtgctgtc gcgcgtcgga
     3241 aagctcgtag aggaaaatcg cttcgcccga ttcggcggcg aactgccttg attgcagtac
     3301 tgccgaaaac ctttccggct tgagcgtgaa agccagggcc gcggccgcgc catgcgggtc
     3361 ggcgtcgccg ggctcgatct gcgtgcatgc ccagcgcttg aacgtcaggc acctgcccag
     3421 gcagcggcgc gaccatgggg tctggttgcg aaacctttcc aggtagggcc ttgactgcag
     3481 tacttcaggc gagtccagct cataggtcgc aagataggtg gcgccgcgca cccagcgccg
     3541 cgcggaacgg aagcccgaga tcgccaggcg ctcggcaagg tgctcgctgt cgtaccaggc
     3601 gttgaactcc tcctccatcg ccggcggcgg ctcggtgagc gtgagaagca ggccgcggct
     3661 catcctgaat tgatcatcct cgaccgatta cgcctttctc cagcacgtac gcccgatcgg
     3721 cgacccggtc ggtgaaatgc cggctctgct cggcgagcag caccgtgacg cccgcgcgct
     3781 tgatctcccg gatggcctgc gccacctgcg ctacgacgag cggggccagt ccttcggaag
     3841 gctcgtccag caggatcgcg gagggattgc ccgccagagt gcgcgcgatg caaagcatct
     3901 gctgctcgcc gcccgacatg cgggcgccgc ggcgatcgcg caagcgggcg agcgcaggaa
     3961 agagcgcaaa cagccgttct tccgtccacg gcggcgcggc gccccgtggc gcctggcggg
     4021 cgacttcgag gttctcgatc acggtcaggt ccgtgaagat acgccgatcc tcggggacgt
     4081 agcccagacc gagacgcgcg atttcgtaag gctcgaggtt gcggatgggc ttggcgtcga
     4141 aaacgatctc gccggagagc cgcggcacga ggcccatgat cgccttgagc gaggtcgact
     4201 tgccggcccc gttgcggccg aggagcgcga cgacttcccc ctggccgacg tcgagatcga
     4261 cgtcgaagag tacctgggcg cggccgtagc cggcgttcag gccgcgcacc tcaagcatcg
     4321 ccaaggtaca cctcgcgtac gcgcgggtcg ccgcgcacct cgtcc
//
LOCUS       DASTPD010000191         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_300679, whole genome shotgun sequence.
ACCESSION   DASTPD010000191 DASTPD010000000
VERSION     DASTPD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300679"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFR57_06335"
     CDS             <1..621
                     /locus_tag="VFR57_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0259019.1"
                     /translation="VVLVLAGDGGTYDIGLQCLSSAAERNENILYVCFDNEGYMNTGA
                     QKSSSTPQFARTGSTPAGKLAPKKNLIEIMAAHRIPYAATATAAFLPDLVRKVTKASA
                     MGGFRLLTLLVPCLDGWGVPDDQGLGVARLAVETGVFPLYEVEDGLRYTVNGPAKTRP
                     VREYLQAQRRYGGLAEAQVDELQREVDADWARLVSKTRPSLVARAP"
     gene            complement(596..820)
                     /locus_tag="VFR57_06340"
     CDS             complement(596..820)
                     /locus_tag="VFR57_06340"
                     /inference="COORDINATES: protein motif:HMM:NF040427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HEU0259020.1"
                     /translation="MLPLLRMLELKNLSMSEAEAGGFGKLAEAAKRCARCTDAMACIR
                     WLKWHGRYGPAPLCVNVAYFDELRARARPG"
     gene            complement(836..1078)
                     /locus_tag="VFR57_06345"
     CDS             complement(836..1078)
                     /locus_tag="VFR57_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259021.1"
                     /translation="MTPFGQALFLVLPAWRRLRDLGLPLWGFVRRRSRLQRVEALRAE
                     RRCRSCQARDRCRGRIIRRLPSPIAGCPNTMLLAMH"
     gene            complement(1140..1382)
                     /locus_tag="VFR57_06350"
     CDS             complement(1140..1382)
                     /locus_tag="VFR57_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEU0259022.1"
                     /translation="MALLINEACTLCDACVPVCPNEAIKAGEPYVIDALKCTECVGAK
                     DEPQCKLVCPSDCIVKNPDFDETPEQLLEKYHALHG"
     gene            complement(1392..2222)
                     /gene="bcrD"
                     /locus_tag="VFR57_06355"
     CDS             complement(1392..2222)
                     /gene="bcrD"
                     /locus_tag="VFR57_06355"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HEU0259023.1"
                     /translation="MTYTVGIDIGSGAVKTVLFNDQQWLAKRVERIRRRDPMELARLG
                     FDEVLREKQLSDKDIAYTATTGEGENVKFATGHFYSMTTHARGGVFLNPQARAVVDIG
                     ALNGRAIYIDERGKVLSYKMTSQCASGSGQFLENIARYLGVAVEEIGPLSRTSKNPEK
                     VSSICAVLAETDVINMVSRGIATPDILKGIHISMASRLVKLLKVTGITKGTVLLTGGL
                     ALDEGLLAALQEELANEKITGLTAVSHPDSIYAGAIGAALWGAFRHARLKELQDRVAA
                     "
BASE COUNT          377 a          770 c          775 g          411 t
ORIGIN      
        1 gtggtgctcg tgctcgccgg cgacggcggc acctacgaca tcggcctgca gtgcctgtcc
       61 tcggccgccg agcgcaacga gaacatcctc tacgtgtgct tcgacaacga ggggtacatg
      121 aataccggcg cgcagaagtc ctcgtccact ccgcagttcg cgcgcaccgg ctcgacgccg
      181 gcgggcaagc tcgcgccgaa gaagaacctg atcgagatca tggccgcgca ccgcatcccc
      241 tatgccgcca ccgccacggc ggcgttcctg cccgatctcg tgcgcaaggt gacgaaggcg
      301 agtgcgatgg gcggtttcag gctcctcacg ctgctcgtcc cctgcctcga cggctggggc
      361 gtgcccgacg accagggcct cggggtggcg cggcttgcgg tcgagaccgg tgtcttcccg
      421 ctgtacgagg ttgaggacgg cctacgctat accgtgaacg ggccggcgaa gacacggccg
      481 gtgcgcgaat atctacaagc gcaacgccgc tacggcggtc tggccgaagc gcaggtcgac
      541 gagctccagc gcgaggtcga cgcagactgg gcgcggctcg tgtcgaaaac gcggcctagc
      601 ctggtcgcgc gcgcgccctg agctcgtcga aataggcgac gttaacgcag agcggcgccg
      661 ggccatatcg cccgtgccac ttgagccagc gaatgcacgc catcgcatcg gtgcagcgcg
      721 cgcagcgctt tgctgcctcc gcgagcttgc cgaagccgcc cgcctccgcc tcgctcatag
      781 acaggttttt gagctcgagc atgcgcagca ggggcagcat ggggatcgtc cgtcctcagt
      841 gcatcgccag tagcatggtg ttcgggcagc cggcgatcgg actcggaagg cggcgaatga
      901 tgcggccgcg gcagcgatcg cgcgcctggc acgacctgca acgccgctcg gcgcgcagag
      961 cttctactcg ctgcaggcgc gaacggcgcc gcacgaagcc ccacagcgga aggccgaggt
     1021 cgcgcagacg ccgccacgcc ggcaggacga ggaacagagc ttgaccgaag ggtgtcatga
     1081 gcgctcaacc accctacgtc ccctgtgcgc ccggaagttt gatctggcgc aaaggtcgtt
     1141 cacccgtgca gcgcgtggta cttctccagg agctgctccg gcgtctcgtc gaagtccggg
     1201 ttcttgacga tgcagtcgga cgggcagacg agcttgcatt gcggctcgtc cttggcgccg
     1261 acacattcgg tgcacttgag cgcgtcgatc acgtagggct cgcccgcctt gatggcctcg
     1321 ttcgggcaca ccggcacgca ggcgtcgcag agcgtgcagg cttcgttgat cagcagtgcc
     1381 atgatgttct cctaagcagc aacccggtcc tgcagttctt tcagccgggc gtgccggaac
     1441 gccccccata acgcggcccc gatcgcgccg gcgtagatcg aatcggggtg gcttaccgcc
     1501 gtaaggccgg tgatcttctc gttggcgagc tcctcctgca gcgccgcgag caggccctcg
     1561 tcgagcgcga ggccgccggt gaggagcacg gtgcctttag taatgccggt caccttcagg
     1621 agcttcacca gtcgtgaagc cattgaaatg tggatccctt tcagaatgtc gggcgtggcg
     1681 atgccgcgcg agaccatgtt gatgacgtcg gtctcggcga gcactgcgca gatcgagctc
     1741 accttctcag ggttcttcga ggtgcgcgag agcggcccga tctcctccac cgcgacgccg
     1801 aggtagcgcg cgatgttttc gaggaactgc cccgagcccg aggcgcactg gctcgtcatc
     1861 ttgtaggaaa gcaccttgcc gcgctcgtcg atgtagatcg ctcggccgtt gagcgcgccg
     1921 atgtccacta cggcgcgcgc ctgcgggttg aggaacaccc cgccgcgcgc gtgcgtggtc
     1981 atggaataga agtgcccggt cgcgaacttg acgttctcgc cttcgccggt ggtggcggta
     2041 taggcgatgt ctttatcgga gagctgcttt tcgcgcagca cctcgtcaaa tccgagcctt
     2101 gcgagctcca tcgggtcgcg ccggcgaatg cgctcgaccc gcttcgcgag ccactgctgg
     2161 tcgttgaaca ggactgtctt cactgctccg gagccgatgt cgatgccaac ggtgtaggtc
     2221 actttctcgc ctccgcggtc ttgccctctt cgacgaccgc gcgccaggcg aaggtcgccg
     2281 cgccgagcgc gccggtgtag atcgaatcgg ggttgatgtt gagcgtcagc tcg
//
LOCUS       DASTPD010000192         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1117910, whole genome shotgun sequence.
ACCESSION   DASTPD010000192 DASTPD010000000
VERSION     DASTPD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117910"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFR57_06360"
     CDS             <1..666
                     /locus_tag="VFR57_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259024.1"
                     /translation="PLIVGFVLAQGAENYLYQAIQFHGWAWVLRPGVLIIAAITAASM
                     WAGARYGRAQIDEGGASARVRDIRPQLAFAIVMTALFAYAVLDLQRWSFLAKAFPLGV
                     AVAALPVALFVAAVICRRSHASAVIFDTEAETTAGVTALGRYLLWIAVLLAASGLAGF
                     VIGLALFFAAFLHLEARAPIARNALLTASAVLFLTAMSHIFVLDFPRGLLQELVEMPW
                     PLR"
     gene            complement(677..1420)
                     /locus_tag="VFR57_06365"
     CDS             complement(677..1420)
                     /locus_tag="VFR57_06365"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEU0259025.1"
                     /translation="MTRPVARFIGRSSLIGLLAFIGASVTAEGYDSLALDPAQASEPV
                     VQVWGARTRGAKGIFGVHTWVAVKPEGAAEFTVYEVVGWRLRWSESALVIRNRAPDSW
                     FGAQGELYAEKRGPGVEELIRRIDKAAHEYPFAKTYSLWPGPNSNTFTAWIARAVPEL
                     ETDLPATAIGKDYIGSDLFASAPSGSGFQFSLRGLFGVAASGVDGFELNVLGLNFGVS
                     PSGIKLPLLGRIGAARIQPAVPAEQPALR"
     gene            complement(1479..2279)
                     /gene="ttcA"
                     /locus_tag="VFR57_06370"
                     /pseudo
     CDS             complement(1479..2279)
                     /gene="ttcA"
                     /locus_tag="VFR57_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            complement(2297..2614)
                     /gene="sugE"
                     /locus_tag="VFR57_06375"
     CDS             complement(2297..2614)
                     /gene="sugE"
                     /locus_tag="VFR57_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEU0259026.1"
                     /translation="MAWLWLTLAGLLEVVWAIGLKYTDGFTRLAPSVITVAAMIGSVY
                     FLALAVRTIPIGTGYAVWTGIGAVGVAILGMILFGEPKTLLRLGSIFLIVIGIAGLKL
                     LTP"
     gene            complement(2625..>2954)
                     /locus_tag="VFR57_06380"
     CDS             complement(2625..>2954)
                     /locus_tag="VFR57_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0259027.1"
                     /translation="INIVDIHAERPLKGYALYSIAKAGLAAMTRSLALELAPRVRVNG
                     VSPGAIAWPEDGQFPDLERARILATTPLARIGSPEDVARAVHFLACAPYVTGQIVAVD
                     GGRSIFI"
BASE COUNT          520 a          949 c          989 g          496 t
ORIGIN      
        1 ccgctaatcg taggcttcgt actcgctcag ggcgccgaga actacctgta ccaggcgatc
       61 cagttccacg gatgggcgtg ggtgctgcgc cccggggtgc tgatcattgc ggcgatcact
      121 gccgcgtcga tgtgggccgg cgcacgctat ggccgagccc agatcgacga aggcggcgcg
      181 agcgctcggg tacgcgatat ccggccgcag ctcgcgttcg cgatcgtcat gacggcgcta
      241 tttgcgtatg cagtgctcga cctgcagcgc tggtcgttcc tggcaaaggc gttcccgctc
      301 ggcgtcgcag tcgcggcgct tccggttgcg ctttttgtcg cggccgtcat ctgtcgccga
      361 tcgcacgcga gcgcggtgat cttcgacacc gaggctgaaa ccactgcggg tgtgaccgcg
      421 ctaggccgtt acttgctttg gatcgccgtg ctgctcgccg cgagcggcct cgccggcttc
      481 gtgatcgggc tcgcgctctt cttcgcggcc ttcctacacc tcgaggcgcg tgcgccgatc
      541 gcgcgaaacg ctctgctcac ggcttcggcc gtgctgtttc tcaccgccat gtcgcacatc
      601 tttgtgctcg acttcccgcg cgggctgctg caggaactag tcgagatgcc ttggccgtta
      661 aggtaggaag ggagcgctac cgcagcgccg gctgctcggc cggaacggcg ggctggatgc
      721 gcgcggcacc gatgcgtccg aggagcggaa gcttgatgcc ggagggactg acgccaaagt
      781 tgagccctag aacgttcagc tcgaagccgt ccaccccgct cgccgcaacg ccgaaaagcc
      841 cacgcagcga gaactggaag ccgctgccgc tgggcgcgct ggcgaataga tccgagccga
      901 tgtagtcctt gccgatcgcg gtcgccggca agtcggtttc gagctcgggc acggcgcgcg
      961 cgatccatgc agtgaatgtg ttcgaattag gccccggcca gaggctgtag gtcttggcga
     1021 aggggtactc gtgcgcagcc ttatcgatgc ggcggatgag ctcctcgacc cccgggccgc
     1081 gcttctcggc ataaagctcg ccttgcgctc cgaaccagga gtcgggtgcg cgattgcgga
     1141 tcacgagcgc actctcggac cagcgcaggc gccagccgac cacctcgtag accgtgaact
     1201 cggcagcccc ctcgggcttg accgctaccc aggtgtgcac gccgaagatg cctttggcgc
     1261 ctcgcgtgcg cgcaccccag acctgcacga ccggctcgct tgcctgcgcg ggatccagcg
     1321 caaggctgtc gtagccctcg gcggtgaccg aggcgccaat gaaagccagc aagccgatga
     1381 ggctcgagcg gccgataaac ctagcgacag ggcgcgtcat gctcgctatt gaaacgcatt
     1441 gtaggctcca ccggatgaca cacgagttat ctgaaattca agaaatcgta gagcttccgg
     1501 tcgaggaggt gcgaaggacg cacctcggtc agcgcgcggg cgatcgactc gatgcgcccc
     1561 ggctgacggc gcttccaatc ccgcagcatc tcgccgatct gtttgcgctc caggttatcc
     1621 tgtgagccgc acaatgtgca gggaatgatc gggaagccct tggctgcggc gtaggccgcc
     1681 agatcgtctt cggcgacgta cgcgagcggc cggatcacca cctgccggcc gtcgtccgag
     1741 cgcagcttgg caggcatcgc tttcagctgc ccgccgtgga acagattgag gaagaaagtc
     1801 gccaggatgt cgtcacggtg atggccgagc gcaatcttcg tcgcgccgag ctcgcccgcc
     1861 acgcggtaca gcacgccgcg acgcagccgc gagcagagcg agcacattgt ctgcccctcc
     1921 ggaatgacgc gcttgaccac agagtaggtg tcctgctcga cgacgcggaa ctcgacgccg
     1981 cgggagcgaa ggtactcggg aaggacatgc cccgggaacc ccggctgctt ctggtccaga
     2041 ttgacagcga tcagctcgaa ctctacaggc gccttttctt gcaaggacaa aaggacgtcc
     2101 aggagtccat gactgtcctt gccgcccgac acgcatacca ttacccggtc gcccgcctga
     2161 agcatgttga agtccgcgat cgcctcgccc acctggcggc gcagccgctt ggcgagcttg
     2221 ccgagctcgt agtcggcctt gcgcgggtcg cggtggatgc ggacaggtcg ggcggacata
     2281 gggaacggga gtgtacctaa ggagtcaaca gcttgagccc ggcgatacca atgacgatca
     2341 gaaagattga accgaggcga aggagcgtct tcggctcgcc gaagagaatc atgccgagga
     2401 tggcgacgcc gaccgcaccg atgccggtcc acacggcgta gccggtgccg atcgggatgg
     2461 tgcgcacggc gagggcgagg aagtagacgc tgccgatcat cgccgccacg gtgatcaccg
     2521 aaggcgcgag ccgggtaaag ccgtcggtgt acttgaggcc gatcgcccag acgacttcga
     2581 gcagaccggc gagcgtcagc cacagccagg ccatgagcgt ttccttagat gaagatcgag
     2641 cggccgccgt ccacggccac gatctggccg gtaacgtagg gcgcgcaggc caggaaatgc
     2701 acggcgcgcg ctacgtcttc cggcgagccg atgcgcgcaa gcggagtggt ggcgaggatg
     2761 cgcgcgcgct cgaggtccgg gaattgcccg tcttcgggcc aggcgatcgc gcccggagag
     2821 acgccgttga cacgcacccg gggcgcgagc tcgagcgcga gcgagcgcgt catcgcggca
     2881 agtcccgcct ttgcaatact gtagagcgca tagcccttga gcggccgctc ggcgtgaatg
     2941 tcgacgatgt tgat
//
LOCUS       DASTPD010000193         5326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_505769, whole genome shotgun sequence.
ACCESSION   DASTPD010000193 DASTPD010000000
VERSION     DASTPD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505769"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFR57_06385"
     CDS             <1..205
                     /locus_tag="VFR57_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259028.1"
                     /translation="QEPPQVHSDALHNPRYATELHPFSGGTLLEIRHPALGWMDFVLP
                     SLERARITRFLTEQEEAWKRFPR"
     gene            236..529
                     /locus_tag="VFR57_06390"
     CDS             236..529
                     /locus_tag="VFR57_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU0259029.1"
                     /translation="MAKAYWVVCYRAIKDSDKLAAYAKLAAPAIQAAGGRFLVRGTPA
                     KTYEGGLNQRTVVTEFESLAKAIAAHDSPGYQEALRVLGDSVERDLRIIEGVS"
     gene            complement(510..1493)
                     /locus_tag="VFR57_06395"
     CDS             complement(510..1493)
                     /locus_tag="VFR57_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEU0259030.1"
                     /translation="MKVAILDDYHDTLRTLQCYGKLAGHDVKVWTDHVQDTDALAARL
                     KDAEALILIRERTKIRAPLLERLPKLRLISQRSVYPHIDIEACTRLGVVVSSNMHPGT
                     PSYSTAEMTWALILAAVRDIPQQAAALKAGRWQIGVGTTLRGKTLGVYGYGRIGAVVA
                     GYGRAFGMQVQVWAREATRAKARADGYAVAPSKEAFFAECDVITLHMRLVEATRGIVT
                     AADLARMKTTALLVNTSRAPLIEPGALVSALKAGRPGRAAVDVYEQEPVTDGRHPLLA
                     MDNVVCTPHLGYVTREEYELQFADIFDQVVAFDAGSPINVVNPEVLRKPLR"
     gene            1517..2104
                     /locus_tag="VFR57_06400"
     CDS             1517..2104
                     /locus_tag="VFR57_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259031.1"
                     /translation="MTQPQLVDPHDPVMTGENSFVRLSNDNGNTIPERVSHWRVLWSP
                     AGQGHALYIESTLLGSKPKIYADNAGVARFLQRTIETLLHKPFADESLPIVDAKFERT
                     GSSLSAVEERIVSAKGEIILAWWDFMAPFVLTMPPGAMDRPLGVYSTFIPARGAQLSV
                     NGEAASGKVFPQERGGRPSSSCVLAWSETWVKPKG"
     gene            2107..2718
                     /locus_tag="VFR57_06405"
     CDS             2107..2718
                     /locus_tag="VFR57_06405"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0259032.1"
                     /translation="MAVLRIAKMGNPVLLQKAAPVEDPTAPEICRLAADMQETIEDIG
                     ASGLAAPQVFVSKRVVVYRIIAARIPPGSGLEPRPWTVMVNPLITPKSESKTPVWERC
                     LSVPGLHGKVPRYMEVEIEYLDLRGKKHRHEATSSWAALLQHECDHLDGIVYPMRMTD
                     LSLLAYNDEPGPLAREVAANPKGIDPLFVDLVKRWPGRSRWFS"
     gene            complement(2772..2918)
                     /locus_tag="VFR57_06410"
     CDS             complement(2772..2918)
                     /locus_tag="VFR57_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259033.1"
                     /translation="MCYEEYWRLEEARREREAEEKRRQAKEQAPAKPAPADKPVEQPD
                     PVPV"
     gene            complement(2949..3824)
                     /locus_tag="VFR57_06415"
     CDS             complement(2949..3824)
                     /locus_tag="VFR57_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0259034.1"
                     /translation="MRPLERIAYSPIIQRAALGLPGGARMIVWTIVNVEQWDIDQPMP
                     RTVLTPPAGGAPMPDIPNWAWHEYGNRVGFWRLTEILDSLDIKAALAINGTAVRAYEP
                     ISRAALERGWEFIGHGYTQRNMQKVENEREDIAKTTEAIRALTGKRPRGWLGPGLTET
                     WETPDLLAEAGYDYVCDWVLDDQPVMLKTRTRPILNVPYTQECNDVAMMLIQHHEAGE
                     YYRRAMDQFEQLYADSRDSARVMALVVHPYIMGAPHRAKYYRRALEEICAHKDVRFWT
                     GEQIADWYLQKGKQA"
     gene            3865..4725
                     /locus_tag="VFR57_06420"
     CDS             3865..4725
                     /locus_tag="VFR57_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259035.1"
                     /translation="MDANFWLIQTFNGVSYGALLFLLASGLTLIFGVMRIVNLSHGAY
                     FLLGGYVALTVIRGTGSWVLALPAAALTLAIVGLLMERIFLRPLGFDPLRQVLLTVGF
                     AFLFQQAALDIWGGDNLIIQPPAALSQSVVVGGLHLPVYRIFMIALAAAIGLALWFAM
                     ERTRIGAVVRATVDDAEMARGVGIDTSKVSMFIFALGAFLAALGGVIGGAFLGIYPGL
                     DFEVLPIAFAVVIIGGMGSLAGAAVGSMLVGLADNFGKALFPEVAYFSLYAPMVLILA
                     LKPTGLMGRE"
     gene            4722..>5326
                     /locus_tag="VFR57_06425"
     CDS             4722..>5326
                     /locus_tag="VFR57_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259036.1"
                     /translation="MKVLILLGALAAVAVIAPFTGPFIILLLAQVLVLGIIAMSVDLL
                     LGYTGLPSLGQAAYLGVGAYLTAILATKFGFGLGWDFWLVVAMGMAAGAATAAFFGLF
                     AIRASGVYFLMITLALGMCVWGLAYRWNSLTGGDNGINMRGRPKLGELAFANDVVFFY
                     VVLGFFLASFFALQLLVSSPFGKSLVGIRERELRMRILGYNT"
BASE COUNT          813 a         1750 c         1744 g         1019 t
ORIGIN      
        1 gcaggagccg ccgcaggtcc acagcgacgc gctgcacaac ccacgctacg ccaccgagct
       61 ccacccgttc tcgggcggca cgctcctcga gatacgccat ccggcgctcg gctggatgga
      121 cttcgtgctg ccaagcctcg agcgcgcccg catcacgcgc ttcctgacgg aacaggagga
      181 ggcctggaag cgcttcccgc gctaaagtta gccggaacct cagggaggaa tgaccatggc
      241 caaggcgtat tgggtggtgt gctatcgcgc aatcaaggac tccgacaagc tcgcggctta
      301 tgcaaagctg gcggcgcctg cgatccaggc agcgggcgga cgttttctcg tacgcggcac
      361 gccggcaaag acttacgagg gcggcttgaa tcagcgaacc gtggtgaccg agttcgagag
      421 cctagccaag gcgatcgcag cgcacgacag tcccggctat caggaagcgc tgcgcgtgct
      481 cggagactcg gtcgagcgcg acctgcgcat catcgaaggg gtttcctaag cacctcggga
      541 ttgactacgt tgattggaga gccggcgtcg aaggcgacta cctggtcgaa gatgtcggca
      601 aactggagct cgtactcttc gcgcgtcacg tagccgaggt gcggggtgca gacgacgttg
      661 tccatcgcga gcagcggatg ccggccgtcg gtcaccggct cttgctcgta gacgtcgacc
      721 gcggccctgc ccggccggcc tgccttcagc gcgctcacca gggcgccggg ctcgataagc
      781 ggcgcgcggc tcgtgttgac aagaagcgca gtcgtcttca tgcgcgcaag atccgctgcg
      841 gtgactatgc cgcgcgttgc ctccacgagc cgcatgtgca gcgtaatgac gtcgcattcg
      901 gcgaagaatg cctccttgct tggcgccacc gcatagccgt ctgcgcgcgc tttcgcgcgc
      961 gtcgcctcgc gcgcccaaac ttgcacctgc atgccgaatg cccggccgta gcccgcgacc
     1021 accgcgccga tgcggccgta gccgtagacg ccgagcgtct tgccgcgaag cgtggttccg
     1081 accccgatct gccagcgccc ggctttcagc gccgcggcct gctgcggaat gtcgcgcacc
     1141 gccgcgagaa tcagcgccca cgtcatctcg gcggtggagt aggacggcgt cccggggtgc
     1201 atgttcgagg acaccaccac gccgaggcgc gtgcacgcct cgatatcgat gtgcggatag
     1261 acgctgcgct ggctgatgag cctgagcttg ggcagccgct cgagcaacgg agcgcggatc
     1321 ttggtgcgct cgcgaatcag aatcagcgcc tcggcgtcct tgaggcgcgc ggcgagggcg
     1381 tcggtgtcct gcacatggtc ggtccacacc ttcacgtcgt ggcccgcgag cttgccgtag
     1441 cactggagcg tgcgcagcgt gtcgtggtag tcgtcgagaa tagcaacctt catgtgagat
     1501 agtttaatct cgcgacatga cccaacctca gctcgtcgat cctcatgatc cggtgatgac
     1561 cggagagaac tcgttcgtgc ggctctcgaa cgacaacggc aacacgattc ccgagcgcgt
     1621 aagtcactgg cgggtgctct ggtcccccgc cggacagggc catgcgctct acattgagag
     1681 cacgctgctg ggctcgaaac ccaagatcta tgcggataac gcgggcgtag cgcgcttctt
     1741 gcagcgcacg atcgagactc tgctgcacaa gccgttcgcg gacgaatcgc tgccgatcgt
     1801 ggatgcgaag ttcgagcgca ctggcagctc gcttagcgcc gtcgaagaac gcatcgtgtc
     1861 ggcgaagggc gagatcatcc tcgcctggtg ggatttcatg gcgcccttcg tcctcaccat
     1921 gccgcctggg gcgatggacc gcccgctcgg cgtctacagc accttcattc ccgcgcgagg
     1981 cgcacagctc tcggtcaacg gcgaggccgc cagcggcaag gtattcccgc aagagcgcgg
     2041 tggccggcct tcttccagct gcgttctcgc ctggtcggaa acctgggtca agcccaaggg
     2101 ctgatcatgg cggtgctgcg gatcgcaaag atgggcaatc cggtgctgct gcagaaggcg
     2161 gcgccggtcg aagatccgac cgcgcccgag atctgtcgcc tcgccgcgga tatgcaggaa
     2221 acgatcgagg acatcggcgc gagcgggctc gccgcgccgc aggtgttcgt gtcgaaacgc
     2281 gtcgtggtct accggatcat cgcggcccgc atcccgcccg gctcgggcct cgagccgcgc
     2341 ccgtggaccg tgatggtgaa tccgctgatc acgcccaaaa gcgagagcaa gaccccggtg
     2401 tgggagcgct gcctttccgt gcccgggctc cacggcaagg tgccgcggta catggaagtt
     2461 gaaattgagt acctcgattt gcggggaaag aagcatcggc atgaggcgac gagctcctgg
     2521 gccgcgcttc tgcagcatga gtgtgaccac ctggacggca tcgtgtatcc aatgcgcatg
     2581 accgaccttt cgctcctcgc ctacaacgac gagcccggcc cgctggcgag agaggtcgcc
     2641 gccaacccca agggcatcga tccgctgttc gtcgacctgg tgaagcgctg gcccggccgc
     2701 agccgctggt tttcctagaa acgagacccc gcctggcggc ggggctcgag gcggttcttc
     2761 tttctgagtc tctagacggg aaccgggtcg ggttgctcga ccggcttgtc agccggcgcg
     2821 ggcttcgccg gcgcctgctc cttggcttgg cggcgcttct cctccgcctc gcgctccctg
     2881 cgcgcctctt caaggcgcca atactcctcg tagcacatag gcacctcctt tcggactgcc
     2941 cgcctatgct acgcctgttt tcctttctgg agataccagt cggcgatctg ctcgccggtc
     3001 cagaaccgca cgtccttgtg cgcgcagatt tcttcgagcg cgcgccggta gtacttcgcg
     3061 cggtgcggcg cgcccatgat gtagggatgc acgacgagcg ccatcacgcg cgctgaatcg
     3121 cgcgagtcgg cataaagctg ctcgaactgg tccatcgcgc ggcggtagta ctcgcccgcc
     3181 tcgtggtgct ggatgagcat catcgcaacg tcgttgcact cctgcgtgta tggcacgttg
     3241 aggatcggcc gggtgcgcgt cttgagcatc acgggctggt cgtcgagcac ccagtcacag
     3301 acgtagtcgt accctgcttc ggccagcagg tcgggcgtct cccacgtttc ggtgaggcca
     3361 ggcccgagcc agccgcgcgg ccgctttccg gtgagggcgc ggattgcctc ggtggtcttc
     3421 gcgatgtctt cgcgctcgtt ctccaccttc tgcatgttgc gctgcgtata gccgtggccg
     3481 atgaactccc agccgcgctc gagcgccgcg cgcgagatcg gctcgtaggc ccgcaccgcg
     3541 gtgccgttga ttgcgagcgc cgctttgata tcgagagaat caagaatttc agtaagccgc
     3601 cagaagccga cgcggttgcc gtactcgtgc cacgcccagt tcgggatatc gggcatcggc
     3661 gcgccgcccg cgggcggcgt gaggacggtg cgcggcatcg gctggtcgat gtcccattgc
     3721 tccacgttga cgatggtcca gacgatcatg cgcgcgccgc cgggaagccc gagcgccgcg
     3781 cgctggatga tgggcgaata agcgatccgc tcgaggggtc tcatggagaa gtgatgctac
     3841 tctttcgccc ggaggaggaa ccgggtggac gcgaacttct ggctgatcca gacgttcaac
     3901 ggcgtgtcgt acggcgcgct gctcttcctg cttgcgagtg gcctcacgct gatcttcggc
     3961 gtgatgcgca tcgtcaacct ctcgcacggc gcctacttcc ttctcggcgg ctacgtggcg
     4021 ctcacggtga tccgcggaac cggctcctgg gtgctcgcgc ttccggcggc ggctttgaca
     4081 cttgccatcg tcggtctcct gatggagcgc attttcctgc ggccgctcgg gttcgacccg
     4141 ctgcgccagg tgctgctcac ggtcggcttc gcgttcctct ttcagcaggc ggcgctcgat
     4201 atctggggcg gcgacaacct gatcatccag ccgccggcgg cgctttcgca gagcgtggtg
     4261 gtcggcgggc tgcatctgcc ggtgtaccgc atcttcatga tcgcgctcgc agcggcgatc
     4321 ggcctcgcgc tctggttcgc catggagcga acgcgcattg gcgcggtggt gcgcgccacc
     4381 gtcgacgatg ccgagatggc gcgcggcgtg ggcatcgaca cgtcgaaggt atcgatgttc
     4441 atcttcgcgc ttggcgcgtt cctcgccgcg ctcggcggcg tgatcggcgg cgccttcctc
     4501 ggcatctatc cgggcctcga cttcgaggtc ctgccgatcg cgttcgcggt ggtgatcatc
     4561 gggggcatgg gttcgctcgc tggcgccgcg gtcggcagca tgctggtggg cctcgccgac
     4621 aatttcggca aggcgctctt tcccgaggtg gcgtacttca gcctgtacgc gccgatggtg
     4681 ctgattctcg cgctgaaacc tacggggctg atgggaagag aatgaaagtc ctcatcctcc
     4741 tgggagcctt ggctgccgtg gcggtcatcg ctcccttcac cggaccgttc atcatcctgc
     4801 tgctcgcgca agtcctggtg ctcggcatca tcgccatgag cgtggatctc ctcctcggct
     4861 ataccggcct gccgtcgctc ggccaggcgg cgtatcttgg cgtgggcgcg tacctcaccg
     4921 cgatccttgc gaccaagttc ggcttcggcc tgggctggga tttctggctc gtggtcgcca
     4981 tgggcatggc cgcgggcgcg gcgaccgcgg ccttctttgg cctgttcgcc atccgcgcga
     5041 gcggcgtgta cttcctcatg atcacgctcg cgctcggcat gtgcgtctgg ggcctcgcct
     5101 accgctggaa ctcgctcacc ggcggcgaca acggaatcaa catgcgcgga cggccgaagc
     5161 tcggcgagct tgcgttcgca aacgacgtcg tcttcttcta cgtggtgctc ggctttttcc
     5221 ttgcctcgtt tttcgcgctg cagcttctcg tgagctcgcc tttcggcaag agcctggtcg
     5281 gcatccgcga acgcgagctg cgcatgcgca tcctcggcta caacac
//
LOCUS       DASTPD010000194         8176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1322999, whole genome shotgun sequence.
ACCESSION   DASTPD010000194 DASTPD010000000
VERSION     DASTPD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322999"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="VFR57_06430"
     CDS             complement(<1..2001)
                     /locus_tag="VFR57_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEU0259037.1"
                     /translation="MSEETIRLHQPAAEVSAEALARAEEMIEREEGVHNKLVGWLAAL
                     VATVAVVMSLFHLYTAYAIVRPEHLRATHVAFVLFLTFLIFPVARRFRHRVMWWDWAL
                     AALGVSTAVYLIAGGDDFFDRSILPSDWDIFFGVVLMLLVLEAMRRTTGWILPVVTGC
                     FLAYAFFGPYLPTPWTHKGYELPRLVGHMYMTLEGIYGTAIAVSSTLIILFTIYGAFL
                     QFSGAGKFFIDFSFSAMGGKPTGAGRTVTLASFLLGGPSGSGVATTVTLGSVAAPMLA
                     KVGYSKEEAGGLLSAGGLGAIISPPVLGAAAFIIAEFLKISYLDVVLMAVIPTGLFYL
                     TLFLMVEIDVRKFGMKDVTFESVESAWSLAKRYWYHFLSLVSIIAFMLVGFSPELSVF
                     WATVVAFATSFLRRDTALLPYEFFRTGRGFLDSGLIKALQGGSMSVLNVAATCAGAGI
                     IVGVVTLTGLGLKFSSIVLAYAGNSLLLTAIYTALIVWIVGLAVPVTASYIICAVIAA
                     PALIALKVPEFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQCWKYTTP
                     AFLVPFMFVLDPSGSGLLLTGSFKTLANADWGSIALVTFTAAVGILALCGAFQGWLFR
                     KTTAYERWMLFVAGVLLVYPKTLFDAAGLGLVVVVLALQWFRR"
     gene            complement(2051..2998)
                     /locus_tag="VFR57_06435"
     CDS             complement(2051..2998)
                     /locus_tag="VFR57_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0259038.1"
                     /translation="MFVARLLALCLVPFAAQAQNLSIATGGTGGVYYPLGGGMAAVLS
                     KYVPGMQATAEVTGGSVANLQLVGTGKPYLAMTMVDAGLDALKGQDKFTGKPVPVRTL
                     MVMYPNRMHVVTIEGTGVTKMADLKGKRVSTGSGGSATEVMAFRVIEAAGLDKDKDMK
                     RERLGVAESANALKDRKIDAFFWVGGLPTAAVSDLAHSPGVKIKLVDHADLVGKMNQK
                     YGNLYVEDRIPKETYKGMDADNRQATVMNLLVAHQNMDEKTAYNIVKTVFEKRDELIR
                     VHKEAENFKLENQKTAAAGGIPWHPGALKYYAEKGVKVN"
     gene            complement(3031..3636)
                     /locus_tag="VFR57_06440"
     CDS             complement(3031..3636)
                     /locus_tag="VFR57_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0259039.1"
                     /translation="MHYDFTTPLTGNDAKKLRVNDTVTLRGTLFGIRDATQIHMFDRG
                     RKTRLDLRGHAVIHTAPNVRKVEKDATNPTGYAPVCVGTTTSDRMERFTAPLMREYGV
                     RLIIGKGGLREGSSKAFQELGGAYLAIIGGTAALETTWVEAIEDVDLDDLHPESLWKF
                     RVNGFGPLLVAMDSHGASLYAQVKAAAQHKRAEALKRLGVR"
     gene            complement(3639..4220)
                     /locus_tag="VFR57_06445"
     CDS             complement(3639..4220)
                     /locus_tag="VFR57_06445"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEU0259040.1"
                     /translation="MVSRFTRTVLVAAALLLAPLQAPAAEVAGVKIDDNARVADSDLA
                     LNGAGLRTRLFFQVYAIGLYLPQKSSTPAAILAQPGPKRVAIHMLRDVGADTFTEALA
                     DGIRANHSEAEAKALEPRIKELAALMAEIKEAKKGMAIALDWTGAGTQLLVQGKPAGR
                     PIAGEDFYRALLRIWLGEKPVQDDLKKALLGGG"
     gene            complement(4210..5085)
                     /locus_tag="VFR57_06450"
     CDS             complement(4210..5085)
                     /locus_tag="VFR57_06450"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0259041.1"
                     /translation="MKVDLVQVEEAAKELYIRALKTLPPDIKRGFEALADAETDAGAK
                     RMLGTMVRNITVAEQTDNLLCQDTGIPVYNVWIGRGVEVDGVGLKEAIRRGCARSTRE
                     YSFRSSIVHPTTRANDQSSTGRGIPVITLDFTSEPDVLSIEMIPKGSGSENNSWLRMA
                     VPAEGVDAIKTFVVDCVLEAGGKTCPPTIVGVGVGGTADLCMHLAKVAATRPLGTRCE
                     DPEGAKLEAALSEAVNVLGVGPQGLGGDSTAFAVHVETAATHITMNPVAVNMQCHSAR
                     RARATISSSGVRYGF"
     gene            complement(5082..6038)
                     /locus_tag="VFR57_06455"
     CDS             complement(5082..6038)
                     /locus_tag="VFR57_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259042.1"
                     /translation="MKRTVAFLFGALIAASALAQPWPQKPVRFIVPFPPGGATDISAR
                     LLGEKLTQIWGQTVVIENRGGAGGGVGAAEAARAAPDGYTLFFPSGSVVTANQHVYAK
                     LAYHPEKDFIPVTKVVSGPQVLTVPANSPFKTVKDLIDHARANPGKLTFGHAGIGSQT
                     HLAAENFVWQAKIDALAVPYKGEGPGLAALVGGETSFFVGNVAASIGHINGGRLRALG
                     VTSKTEAAQLPGVPPIAKTIPGFENTGWFGIVAPAGTPNDIVQKVYRDTKKALEASDL
                     RGRLFVQGLAPVGNTPAEMAKELKEESALWARVVKERKIQVK"
     gene            complement(6061..6369)
                     /locus_tag="VFR57_06460"
     CDS             complement(6061..6369)
                     /locus_tag="VFR57_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU0259043.1"
                     /translation="MRQHSSYWMFMVHRISGIALAIFLPLHFWTLGHALQDSLAWTEQ
                     PLVKLGEWLIVAALATHLGGGLRVLALEFLPWRDWQKSLTAAAAAAAAAVGLALALAL
                     "
     gene            complement(6366..6704)
                     /locus_tag="VFR57_06465"
     CDS             complement(6366..6704)
                     /locus_tag="VFR57_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU0259044.1"
                     /translation="MNYRAESFLWLLQRASAAVLAFCVAVHLITIIYAVQGGLTGEEI
                     LARTRGSVGWFVFYSLFVLAVTVHAPIGLRAVLGEWLGWRGRSRDLGLLGFSGFLAWA
                     GMSAVVAVFR"
     gene            complement(6701..7396)
                     /locus_tag="VFR57_06470"
     CDS             complement(6701..7396)
                     /locus_tag="VFR57_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU0259045.1"
                     /translation="MKVLVKRGDSYQEFEVPRQPSQTVLDVVTYIQRKLDPTLAYRFA
                     CRVGMCGSCAMTVNGVPRWTCRTHVDKVLERNTITVAPLRNLPVIRDLVVDMREFFDK
                     WARAKGEFRGRQTRQDDFASVSPQSEDRKLADAGIECIGCAVCYAACDVVKWNPDYLG
                     PAALNRAWTLINDVRDNEPLERLRAVSGDAGCHACHSHVSCTERCPKHLSPTAAIAGL
                     KREATKAMLRGEL"
     gene            complement(7393..>8176)
                     /locus_tag="VFR57_06475"
     CDS             complement(7393..>8176)
                     /locus_tag="VFR57_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactarate dehydratase"
                     /protein_id="HEU0259046.1"
                     /translation="YLQWASELRREERPLKDLWVSTKCGESDTTSGCGANPTVGNAFD
                     KMYPHGVTMVFGETTELTGGEHLVAARCRTDEVRKKFQFVFDRYQQVIERHKTSDLMD
                     SQPTKGNIEGGLTTIEEKALGNIQKIGRKCLVDGVLDKAETPSGPGLWFMDSSSAAAE
                     MVTLCAAAGFAVHLFPTGQGNVIGNPILPVIKLSANPRTVRTMSEHIDVDVSGLLRKE
                     LTPDQAGDKLLECMFRTANGRLTAAEALGHREFVLTRLYESA"
BASE COUNT         1432 a         2726 c         2634 g         1384 t
ORIGIN      
        1 ccttcggaac cattgcaggg cgagcaccac gactacgagc ccgagtccgg ctgcgtcgaa
       61 aagcgtcttc ggatacacca acaggacccc ggcgacgaag agcatccagc gctcgtacgc
      121 cgtggtcttg cggaacagcc agccctggaa cgcgccgcaa agggccagga tcccgaccgc
      181 ggcggtgaag gtgacgagcg cgatcgatcc ccagtctgcg ttggcgagcg tcttgaacga
      241 tccggtgagg agcagcccgc tgccgctcgg gtcgaggacg aacatgaagg gcacgaggaa
      301 cgccggcgtc gtgtatttcc agcactgcag ggtggtcttg tagggatccc cgccggtgat
      361 cgcggccgcg gcgaacggcg aaagcgccgt cggcggcgaa acttccgaca gcaccgcgta
      421 gtagaagatg aacatatgcg ccgcgaactc cggcaccttg agcgcgatca gcgccggcgc
      481 ggcgatcacc gcgcagatga tgtaggacgc ggtcaccgga accgcgaggc cgacgatcca
      541 cacgatgagc gcggtgtaga tcgcagtgag gaggagcgag ttgcccgcgt aggcgagcac
      601 gatcgagctg aacttgaggc cgaggcccgt gagcgtgacg actccgacga tgatcccggc
      661 gccggcgcag gtggccgcga cgttcagcac cgacatcgac ccgccctgca gcgccttgat
      721 taagccggag tcaagaaatc cccgtccggt acggaaaaac tcgtacggaa gaagcgccgt
      781 gtcgcggcga aggaaactcg tggcgaacgc gaccacggtc gcccagaaca ccgagagctc
      841 cggagagaac ccgaccagca tgaaggcgat gatcgagacc agcgacagga agtggtacca
      901 atagcgcttc gccaggctcc aggcgctctc cacgctctcg aacgtcacgt ccttcatgcc
      961 gaacttgcgc acgtcgatct cgaccatgag gaagagcgtg agatagaaca ggccggtcgg
     1021 gatgaccgcc atcagcacca cgtcgaggta ggagatcttc aggaactcgg cgatgatgaa
     1081 cgccgccgcg ccgagcaccg gcggcgagat gatcgcgccc aggccgcctg cggacagcag
     1141 cccgcccgcc tcctccttgc tgtagccgac cttcgcgagc atcggcgccg ccaccgagcc
     1201 gagggttacc gtggtcgcca cgcccgatcc cgacggccca ccgaggagaa acgaggcgag
     1261 cgtgaccgtg cgcccggcgc cggtcggctt gccgcccatc gccgagaacg agaaatcgat
     1321 gaagaacttg cccgcacccg agaactgcag gaacgcgccg tagatggtga acaggatgat
     1381 cagcgtagac gacacggcga tcgccgtgcc gtagataccc tcgagcgtca tgtacatgtg
     1441 gccgaccagc cgcggcagct cgtaaccctt gtgcgtccag ggcgtcggca ggtaggggcc
     1501 gaagaatgcg taggccagaa agcacccagt aacgacggga aggatccacc ccgtggtgcg
     1561 ccgcatcgcc tcgagcacga ggagcatcag caccacgccg aagaagatgt cccagtcgct
     1621 cggcaggatc gagcggtcga agaagtcgtc gccgcccgcg atgaggtaca ccgcggtcga
     1681 gaccccgagc gcggcgagcg cccagtccca ccacatcacg cggtggcgga agcggcgcgc
     1741 caccgggaag atgagaaagg tgaggaacag cacgaacgcc acgtgcgttg cacgaaggtg
     1801 ttcgggacgc acgatggcgt acgcggtgta gaggtggaac agcgacatca cgaccgccac
     1861 cgtggccacg agggccgcga gccagccgac cagcttgttg tgcacgccct cttcgcgctc
     1921 gatcatctcc tcggcgcgcg cgagcgcctc cgcgctcacc tcggcggccg gctggtgcag
     1981 tcggatggtt tcttcgctca taacgaaaaa gccccggcgt acggggcttt ctcgggagtc
     2041 tccctcgctc ctagttgacc ttgacgcctt tctcggcgta gtacttcagc gcgcccggat
     2101 gccacggaat cccacccgca gcggcggtct tctgattctc gagcttgaag ttctccgctt
     2161 ccttgtgcac gcggatcagc tcgtcgcgct tctcaaacac cgtcttcacg atgttgtagg
     2221 cggtcttctc gtccatgttc tggtgcgcga ccagcaggtt catcaccgtc gcctgccggt
     2281 tgtcggcgtc catgcccttg taggtttcct tcggaatgcg gtcctcgacg tagaggttgc
     2341 cgtatttctg gttcatcttc ccgactaggt cggcatgatc gacgagcttg atcttgacgc
     2401 caggcgagtg cgcgagatcg ctcaccgccg cggtgggcag gccgccgacc cagaagaacg
     2461 cgtcgatctt gcggtccttg agcgcgttgg ccgactcggc gacccccagg cgctcgcgct
     2521 tcatgtcctt gtccttgtcg agcccggcag cctcgatcac gcggaacgcc atcacctcgg
     2581 ttgcgctgcc gcccgagccg gtggagactc gcttgccctt gaggtcggcc atcttggtga
     2641 cgccggtgcc ctcgatcgtc accacgtgca tgcggttcgg gtacatcacc atgagcgtgc
     2701 gcacggggac cggcttgcca gtgaacttgt cctggccttt cagcgcatcg agccccgcgt
     2761 ccaccatcgt catcgcaaga taaggcttgc cggtgccgac gagctgcagg ttggcgactg
     2821 aaccgcccgt aacttcggcg gtcgcctgca tgcccggcac gtacttcgaa agcaccgcag
     2881 ccataccgcc gccgagcggg tagtaaacgc cgccggtgcc gccggtggcg atggaaaggt
     2941 tctgcgcctg cgccgcgaac ggcaccaagc aaagcgcaag caagcgcgcg acgaacatga
     3001 tctcactcct cctatggggt ttcggaaatt ctaccgcacg cccaggcgct tcaacgcctc
     3061 ggcgcgcttg tgctgcgccg cagccttcac ctgcgcgtac aggcttgcgc cgtggctgtc
     3121 catggcaacg agcaagggcc cgaaaccgtt gacacggaac ttccacagcg actccgggtg
     3181 caggtcgtcg aggtccacgt cctcgatcgc ctcgacccag gttgtctcga gggcggccgt
     3241 gcctccgatg attgcaagat acgcgccgcc gagttcctgg aatgctttcg aggatccttc
     3301 cctcaggccg cccttgccga tgatcaggcg cacgccgtat tcgcgcatca gcggcgcggt
     3361 gaagcgctcc atgcgatcgg aggtcgtggt gccgacgcag accggcgcgt agcctgtagg
     3421 gtttgttgcg tctttctcga ccttccggac gttgggcgcg gtatggatta cggcgtggcc
     3481 gcgcaggtcc aaacgcgtct tgcggccgcg gtcgaacatg tggatctgcg tcgcgtcgcg
     3541 gatcccgaag agcgtgccgc gcagcgtcac ggtatcgttg acgcgcagtt tcttggcatc
     3601 gtttcccgtc agcggcgtgg tgaagtcgta atgcaccgtc agccgccgcc gagcagcgcc
     3661 ttcttgaggt cgtcctgcac gggcttctcg ccgagccaga tgcgcagcag cgcgcggtag
     3721 aaatcctctc ccgcgattgg acggccggcg ggcttgccct ggaccagaag ctgagtgccc
     3781 gcgccggtcc agtcgagcgc gatcgccatg cccttcttcg cctccttgat ctcggccatg
     3841 agggcggcga gctccttgat gcgcggctcg agcgccttcg cctcggcctc ggaatggttg
     3901 gcgcggatgc cgtcggcgag cgcctcggtg aacgtatcgg cgccgacgtc gcgcagcatg
     3961 tggatggcga cgcgcttggg cccgggctgc gccaggatcg ccgccggggt cgagctcttc
     4021 tgcggcaggt aaagcccgat ggcgtacacc tgaaagaaaa ggcgtgtacg caagcccgca
     4081 ccgttcagcg cgagatcgga atccgccacg cgcgcgttgt cgtcgatctt gacgccggcg
     4141 acttccgccg ccggcgcttg gagcggggca aggagaagcg cggcagcgac aagtacagtc
     4201 cgcgtgaatc tagaaaccat agcgcacccc agaggagctg atggtggcgc gcgcgcggcg
     4261 cgccgagtgg cactgcatgt tcaccgcgac cgggttcatg gtgatgtgcg tcgccgcagt
     4321 ctcgacatgc actgcgaatg cggtggagtc gccgccgagc ccctgtgggc cgaccccgag
     4381 aacgttaacc gcctcgctga gcgcggcttc gagcttggct ccctcgggat cctcgcagcg
     4441 cgtcccgagc ggccgcgtcg ccgccacctt ggcgaggtgc atgcaaaggt cggcggtgcc
     4501 tccgacaccg acgccgacga tggttggcgg acacgtcttg ccgccggctt ccagcacgca
     4561 gtcaacgacg aaagtcttga tggcgtcgac gccttccgcg ggcacggcca tgcgcaacca
     4621 ggagttgttt tccgacccgg accctttggg aatcatttca atggaaagaa catccggctc
     4681 cgaagtgaaa tcgagagtta tgacgggtat tccccttccg gtcgagctct gatcgttcgc
     4741 gcgcgtggtc gggtgcacaa tcgaggaacg gaacgagtat tcgcgcgtcg agcgcgcgca
     4801 gccgcggcgg atggcttcct tcaggcccac accgtccacc tccacgccgc ggccgatcca
     4861 gacgttgtac acgggtatgc cggtgtcctg gcacagcagg ttgtcggtct gctccgccac
     4921 cgtgatgttg cgcaccatgg tgcccagcat gcgcttcgcg cctgcgtcgg tctcagcgtc
     4981 ggcgagcgcc tcgaagccac gcttgatgtc cggcggcagg gtcttcagcg cacggatgta
     5041 cagctccttc gctgcttcct ctacctgaac aagatcaact ttcacttgac ttgaatcttg
     5101 cgctccttca cgactcgcgc ccagagcgcg ctctcttcct tcaactcctt cgccatctcg
     5161 gcgggggtgt ttcccaccgg cgcgagcccc tgaacgaaga gccggccgcg caggtcgctc
     5221 gcctcgagcg ccttcttagt atcgcggtac accttctgca cgatgtcgtt cggcgtgccg
     5281 gccggcgcga cgatgccgaa ccagccggtg ttctcgaagc ccgggatggt cttcgcgatc
     5341 ggcggcacgc cggggagctg cgccgcctcg gttttgctcg tcacgccgag cgcgcgcagc
     5401 cggccgccgt tgatgtgccc gatcgaggcg gccacgttgc cgacgaagaa gctggtctcg
     5461 ccgcccacca gcgcggcgag accggggcct tcgcccttgt aaggcaccgc caacgcgtcg
     5521 atcttcgcct gccagacgaa gttctcggcc gcgaggtgtg tctgcgagcc gatgccggcg
     5581 tggccgaatg tcagctttcc agggttggcg cgcgcgtgat cgatgaggtc cttcaccgtc
     5641 ttgaagggcg agttcgccgg cacggtcagc acttgcggcc ccgacaccac cttggtgacc
     5701 ggaatgaagt ccttctccgg gtggtatgcg agcttcgcgt acacgtgctg gttcgcggtg
     5761 acgaccgaac ccgatggaaa gaagagcgtg tagccgtccg gcgcggcgcg cgccgcttcg
     5821 gcggcgccca caccgccgcc cgcaccgccg cggttctcga tcaccaccgt ctggccccag
     5881 atctgcgtca gcttctcgcc gagcaggcgc gccgagatgt cggtagcgcc gcccggcggg
     5941 aacggcacga tgaagcgcac cggcttctgt ggccacggct gcgcgagcgc gctcgccgcg
     6001 atgagcgcac cgaacaggaa agcgacagtc cttttcatgg aatcctcctg ccggaggatt
     6061 ctacagcgcc agcgcgagcg cgagcccgac cgcagccgca gccgcggcag ccgcggccgt
     6121 aaggctcttc tgccagtctc gccagggcag aaactcgagc gcgagcacgc gcagcccgcc
     6181 gcccagatgc gtggcaagcg ccgccacgat cagccactcg ccgagcttca cgagaggctg
     6241 ctcggtccag gcgagcgagt cctgcagcgc atggcctagc gtccagaagt gcagcggcag
     6301 gaaaatagca agcgcgatcc cggagatgcg gtgcaccatg aacatccagt aggacgaatg
     6361 ctgtctcatc ggaagactgc cacgactgca ctcatcccgg cccatgcgag gaagccggag
     6421 aatccgagca agccgagatc tcttgagcgg cccctccagc cgagccattc gccgagcacc
     6481 gcgcgcaagc cgatcggcgc atgcaccgtg accgcgagca cgaagagcga atagaacacg
     6541 aaccagccga cgctgccacg cgtgcgggcg aggatctcct cgcccgtgag gccgccctgc
     6601 accgcgtaga tgatcgtgat cagatgaact gcgacgcaga aggcaagcac cgccgcactt
     6661 gcgcgctgga gcagccacaa aaacgattca gcgcggtagt tcacagttcg ccgcgcagca
     6721 tcgccttggt cgcttcccgc ttcaaccccg cgatcgcggc ggtgggagaa agatgcttcg
     6781 ggcagcgctc ggtacagctc acatggctgt gacaggcatg gcagccggcg tcgccggaca
     6841 ccgcgcgcaa ccgctcaagc ggctcgttgt ctctcacatc attgatcagc gtccaggcgc
     6901 ggttcaacgc cgccgggccg agatagtcgg ggttccactt caccacgtcg cacgcggcgt
     6961 agcacacggc gcacccgatg cactcgatgc ccgcatcggc aagcttgcgg tcttcggatt
     7021 gcggcgatac ggaggcgaaa tcatcctgcc gggtctgccg cccgcggaac tcgcccttgg
     7081 cgcgcgccca cttgtcgaaa aactcacgca tgtccacgac gagatcgcgg atcaccggga
     7141 gattccgcaa tggcgccaca gtgattgtgt ttctttcgag aaccttgtcc acgtgcgtcc
     7201 ggcatgtcca tctcggcacg ccgttcaccg tcatggcgca cgagccgcac atgccgacgc
     7261 gacaggcgaa gcggtacgcg agcgtcgggt cgagcttgcg ctggatgtaa gtcaccacat
     7321 ccagcacggt ctggctcggc tgccgcggca cctcgaactc ctggtacgaa tctccccgct
     7381 ttacgagaac cttcaagcgc tctcgtaaag acgcgtcagc acgaactcgc ggtgacccag
     7441 agcttccgcg gccgtgaggc ggccgttggc ggtgcggaac atgcactcga gcagcttgtc
     7501 gcccgcttgg tcgggcgtca gctcttttct gagcaggccc gagacgtcca cgtcgatgtg
     7561 ctcggacatg gtgcgcacgg tgcgcgggtt cgcgctcagc ttgatcaccg ggaggatggg
     7621 gttgccgatc acgttgccct gacccgtggg gaagaggtgc accgcgaagc ccgcggccgc
     7681 gcacaacgtc accatctccg ccgcggcgga ggacgaatcc atgaaccaca ggccgggccc
     7741 gctcggcgtc tccgccttgt cgagaacgcc gtccaccagg catttcctgc cgatcttctg
     7801 gatgttgccg agcgcctttt cctcgatcgt ggtcagcccg ccctcgatgt tgcccttggt
     7861 gggctgcgag tccatgaggt cgctcgtctt atggcgctcg atgacctgct ggtagcgatc
     7921 gaagacgaac tggaatttct tgcgtacctc gtctgtcctg cagcgcgcgg cgaccaggtg
     7981 ctcgccgccg gtgagctcgg tggtctcgcc gaagaccatg gtcacgccgt gggggtacat
     8041 cttgtcgaac gcgttgccta cggtgggatt ggcgccgcag cccgaagtgg tatcggactc
     8101 gccgcatttc gtggacaccc agaggtcctt gagcggccgc tcctcgcggc gcagctcgct
     8161 cgcccattgc aggtat
//
LOCUS       DASTPD010000195         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_507436, whole genome shotgun sequence.
ACCESSION   DASTPD010000195 DASTPD010000000
VERSION     DASTPD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507436"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFR57_06480"
     CDS             complement(<1..659)
                     /locus_tag="VFR57_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259047.1"
                     /translation="MRGLKGKTAIVTGGAAGIGAAVVQRFKDEGTRVIVFDLKGDPAI
                     DITDYAAVKKAVDSAGPVDILVNNAGWDMFKPFLKTDPAFWQKIISINLLGAMNLLHC
                     VLPGMAERGRGKVVTVASDAGRVGSSGEAPYSACKGGLIALTKTLAREMASKNIQLNV
                     VCPGLTETNMLEQFMQGAGNPEKLREAYRRAIPIGRLGKPDDLPGAILFFASDDADFI
                     TG"
     gene            complement(661..1059)
                     /locus_tag="VFR57_06485"
     CDS             complement(661..1059)
                     /locus_tag="VFR57_06485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0259048.1"
                     /translation="MKVLLPPGWKPPVGYTNGIESSGRLVFVAGQVGWNAEQVFESED
                     LAPQFEQALKNIVAVLAEAGARPEQICRMTAYCCDKPAYLAARPALGKIWRKVIGRHY
                     PAMSMIFVSDLLDSPGKIELEATAVIPEER"
     gene            complement(1059..1577)
                     /locus_tag="VFR57_06490"
     CDS             complement(1059..1577)
                     /locus_tag="VFR57_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEU0259049.1"
                     /translation="MKTKSQPSTQYADFMSRAYTMELEATERYTQFAEQLETHNNREV
                     ASLFRKLAEIEKLHAKRILEEMQWPSVPALAPAYAWEGAEGPETAPFDSLHYLMQPWH
                     ALEIALRCEREAQKYYAGIAASRAPKPVRDAAREMAAEEAEHVRLIKAWMTRVPKPPA
                     GWDEDPDPPGVR"
     gene            complement(1574..>2005)
                     /locus_tag="VFR57_06495"
     CDS             complement(1574..>2005)
                     /locus_tag="VFR57_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259050.1"
                     /translation="KGQPSPTTEPDWRSKLAPGGTGVREFHPLVIALASGANFVARAF
                     SGDPNGCAALIAEAVRHPGFSFVEVLSPCVTFRPDQRGWKELVHPAPVAPTDDPARAA
                     RRIMTDDGFNIGVLYKGNRPVYAPPASKGGTVADLEKAFQL"
BASE COUNT          308 a          650 c          675 g          372 t
ORIGIN      
        1 ccggtgatga agtcggcgtc gtcgctcgcg aagaagagga ttgcccccgg caggtcgtcg
       61 ggcttgccca ggcggccgat cgggatcgcg cgccggtagg cctcgcgcag cttctcgggg
      121 ttgcccgcgc cctgcatgaa ctgctcgagc atgttggttt ccgtgaggcc cgggcacacc
      181 acgttcagtt gaatattctt cgaagccatc tcgcgtgcga gcgttttcgt gagagcaatc
      241 aagccgcctt tgcaggcgga gtagggcgct tcgcccgacg agcccacgcg gcccgcgtcg
      301 gaagcgaccg tgacgacctt gccgcgcccg cgctcggcca taccgggcag cacgcagtga
      361 aggaggttca tcgcgcccag caggttgatc gagatgatct tctgccagaa cgcgggatcg
      421 gtcttgagga acggcttgaa catgtcccag ccggcattgt tgacgaggat gtcgaccggg
      481 ccggcgctgt cgacagcttt cttcaccgcg gcatagtcgg tgatgtcgat ggctggatcg
      541 cccttgagat caaagacgat gacgcgcgtg ccttcatcct tgaaccgctg taccacggct
      601 gccccgatgc cggcggcacc tccggtgacg atggcggttt ttcctttgag gcctctcatt
      661 tcatctctcc tcgggaatca cggcggtcgc ctcgagctcg atcttgcccg gcgaatctag
      721 caggtccgag acgaagatca tgctcatcgc cgggtagtgg cgcccgatga cctttctcca
      781 gattttcccc agcgcggggc gcgcggccag ataggccggc ttgtcgcagc agtaagcggt
      841 cattcggcag atctgctcag gccgtgcacc ggcttcggcc agcaccgcga cgatgttctt
      901 cagtgcctgc tcgaattgcg gtgcgagatc ttcagactca aaaacctgct cggcgttcca
      961 cccgacctgg cctgcgacga aaaccaggcg gccggagctc tcgatgccgt tcgtgtagcc
     1021 gaccggcggc ttccagcccg gcgggagaag cactttcatc aacgcacgcc gggcggatcg
     1081 ggatcctcgt cccagccggc cgggggcttc ggcacgcgcg tcatccacgc cttgatcagg
     1141 cgcacgtgct ccgcctcctc ggcggccatc tcgcgcgcgg cgtcgcgcac cggcttgggc
     1201 gcgcggctcg cggcgatgcc tgcgtagtat ttctgcgcct cgcgttcgca gcgcagcgcg
     1261 atctcgagcg cgtgccaggg ctgcatcaga tagtgcagcg aatcgaacgg cgcggtctcg
     1321 ggaccctcgg caccttccca agcataggcg ggcgcaagcg ccggcacgct gggccattgc
     1381 atctcttcga ggatccgctt cgcatgcagc ttttcgatct cggcaagctt gcggaagagg
     1441 ctcgccacct cgcggttgtt gtgcgtctcg agctgctcgg cgaattgcgt gtagcgctcg
     1501 gtcgcctcga gctccatggt gtaggcgcgc gacatgaagt cggcatattg cgtggaaggc
     1561 tgggatttcg ttttcacagc tgaaacgcct tttccaggtc cgccaccgtg ccgcctttcg
     1621 aggcaggcgg cgcgtacacg gggcggttgc ccttgtagag cacgccgata ttgaagccgt
     1681 cgtcggtcat gatgcggcgc gcggcgcgcg ccgggtcgtc ggttggcgcc accggcgcgg
     1741 gatgcacgag ctccttccag ccgcgctggt ccgggcggaa ggtgacgcac ggcgagagca
     1801 cctcgacgaa cgagaatccc gggtggcgca ctgcttcggc gatcagcgcg gcgcagccgt
     1861 tcggatcacc cgagaaggcg cgcgccacga agttcgcgcc cgaggcgagc gcgatcacga
     1921 gggggtggaa ctcgcgcacg ccggttccgc cgggcgcgag cttcgaacgc cagtcgggct
     1981 cggtggtggg cgagggctgg ccctt
//
LOCUS       DASTPD010000196        10748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_98262, whole genome shotgun sequence.
ACCESSION   DASTPD010000196 DASTPD010000000
VERSION     DASTPD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98262"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFR57_06500"
     CDS             complement(<1..1323)
                     /locus_tag="VFR57_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259051.1"
                     /translation="MKTCANLIAAALTVVTLIAAGGAYGQISFRGAASATAQGITPTF
                     RAAASAASRAGNLSINKPAGVAAGDVLIASVAVRPSSAVITPPAGWTLVRRLDNPGSS
                     SNSLAVFHKVALTVEPASYVWDVPGASFSVGGIQAFSGIDTANPIDAENGQNTPLGTI
                     HAAPSITTSIANTMLVSAHAFASSETWDPPGGMTESVDRRSEGGPGGISIEVNRALQS
                     VAGSTGSKAATADGKADVGNAHLLALRPAGANLSIATPAGTVPGDVMVAAIAFNHSSA
                     FATPPAGWTNVRRMNNAAQTSNVLAIYSRTAAPGEPATHVWRIAGGTFIVGGIQSFSR
                     VDTANPIDAESGHSTPSSTSHDTPSITTSAANAMLVTAHSYAAAQSWTAEAALTEAFD
                     RAIGLGGIGQSITGTYQLQATAAPTGAKRSTAAASSDVGNAHILALRPA"
     gene            complement(1339..4674)
                     /locus_tag="VFR57_06505"
     CDS             complement(1339..4674)
                     /locus_tag="VFR57_06505"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEU0259052.1"
                     /translation="MTDDGNCVCNPGFEQAENSNSCVANLALAKNNGSCTDPSARGTS
                     TVGNPCNPATGNKFEQQSVYRGLAGFELALSFNSFDVSGLRFGTRWRDSFDRRISLVR
                     TAAVAFRPDGRALRFTPNGGAWITDADTADRLVEVQNPPGTRTGWQLAAANADEFETY
                     DAAGRLLAIRSRNGLTQTMAYSDGTGGPNGGFVLDANGNATTSVLPAGLLIRASDHFG
                     RVLAFGYDITSRVTKLTDPGGGVYRFVYVAGKLTSITFPDSVTRTYLYNEPPHTGGAD
                     LPDALSGIVDENGDRFATFQYDAQQRAITTEHAGGAMRHALLYGDGSTTVTGPLGAVR
                     EYGFTTLFDVRKTTGISGAVCPECGPASQTFDANGNVAARVDWNGNRTDYAYDLTRNL
                     ETSRTEGLTASGDPTPLTRTTTTRWHAVFRLPVSIAEPLRMTSFVYDADGNQCGARGV
                     LCSKSIQATTDTNGSTGFSASPSGPPRTWAYTYNANGVVLSIDGPRTDVPDVTTYRYY
                     ANDDSDPGKRGNVQTVTNAAGHQTSITAYNAHGQPLAIVDANGLTTTLFYDARQRLKA
                     RTVGNESTTYDYDNVGQLTKVTLPDGSFLLYRYDAAHRLTGIEDSVSNRIAYTLDAMG
                     NRTLEEVRNVTSELVQTRSRVINALNRVFRELGAEGQTTEYSYDNQGNVLAVKDPLSR
                     VTLNEYDPFHRLKQVTSPAPIFAVTQYGYNGLDALTSVTDPRSLVTSYSVDGLGNVVE
                     QLSPDTGATASVYDAAGNLLGQTDAKNQTTQYAYDALNRVLLITFHDGARHSFGYDQG
                     PNAAGRLSSIAEINHANQVTSSIHYTYDLHGRVTSETRTVAGVAHFMAYRYDAFGRLD
                     QLTYPSGRTVNYTFDPLGRVSSVTTTRSGGQPQTVLAGVAYHPFGGVKGYTFGNGQIY
                     SRDIDQDGRIATYTLGGQIFLIGYDQASRIRSIAQAGNPANTNTYDYDNLDRLTAALV
                     PGTAYSYEYDAIGNRLMRLAGASVHTYAYSATSNRILSITPSSGPVRNFVFDPNGSTT
                     ADGINTYGYDARGRMVHAASVIGITDYQVNALGQRIRKTNTLGDTVFHYDTQGRLIAE
                     TDASGAVKRELIYLGDIPVGIVQ"
     gene            complement(5341..7389)
                     /locus_tag="VFR57_06510"
     CDS             complement(5341..7389)
                     /locus_tag="VFR57_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0259053.1"
                     /translation="MFNLLRYSQLIALAVPIAVVVALTYLYRGLVFDSMVEGETHANV
                     ALTRTFANAIWPAHAAFVRRAGALERPALASRAEIGVLDGDLRRLASGLDIVKVKIYD
                     LHGLTVYSTDPRQIGQSAAGNPGFRRARDGYPVSNLTYRERMDSWEGEIAQRHILATY
                     VPVHVHEAAPVEAVLEIYSDVTRLVEANQRSEWKVLAAVLGAMALLYAVVQLMLARYR
                     RLLEEKERERAAQEERIRHQAYHDPLTGLPNRASFTEHLEEAMRRAKRAGWPLALLFL
                     DLDLFKRVNDCLGHDAGDRLLRVAAERIRRAVREADMLFRMGGDEFTVLLEDVRGPEE
                     AAMVATRVLETIAEPLQLQHHEIAVTSSVGIALYPRDDVVGERLVKAADTAMYRAKEL
                     GRNRYAFFAREMNERVETQMMVEAALRRALKNEEFVLYFQPRVSAATGRATGAEALLR
                     WKHPEWGLVEPARFVPLLEETGLIVPVGAWVLAEACRAARAWQSAGLPPLRVSVNLSS
                     RQFRSEALCDAVSEALRSSGLAPQLLELELTESLLIENVEHAMGVMGQLKAIGTVISI
                     DDFGTGYSSLGYLKRFPIDSLKIDRSFVRDIATSPKDAAIVKAISALARSLGIGLIAE
                     GVEEPWQVEFLRGRYCTELQGFFFSRPLPEEALVEALRRSYPLTLSRRAAVGDEQPVH
                     "
     gene            complement(7455..9194)
                     /locus_tag="VFR57_06515"
     CDS             complement(7455..9194)
                     /locus_tag="VFR57_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0259054.1"
                     /translation="MNKPVKYTLLGLGLVIVLVIGAALVFALTFDPNRYKDEIERLAK
                     EQTGRTLAIKGDIKMAFWPSLGADVSGVTLSERASNQQFLAFDSAHASVKLMPLLRGQ
                     YIVDSVQLSGFKARIVKGKDGRYNFSDLVEADAKKPAPKQEPKKGGGAVVFDIGSIDI
                     QRASIAYSDLQAGQEYMLEDVRIKTGRIASDAEGKLELGMLAKRKAPPLEARLALDGR
                     YRFTNGTLSADVNGKLDESTLKAKFTLAEPYEFEASIDKINLDRYLGAPEKPAAKPAP
                     QQTAKSEDAPVDFSALKGINAKGKLQVGAFEVRGLKLSELNAQLNAANGRVAVAPHSA
                     RGYEGTISGEVIVDANKNHVTLKEKLEGISIGPILRDFAQQDRLEGKGNLTLDVTTAG
                     NTVNAMKRALGGTARVQLREGAVKGINLGEILRKARSTLGGGSQAAQAESGGSGKPQT
                     DFSELSASFTIKNGIAHNEDLDAKAPLFRLGGSGDINIPESTLNYVVKAAIVATAEGQ
                     GGRERDKLAGLTVPVRLHGSFDDLKYDVDLRAMAGNAAKSQVGERVKEQIESRKDKIE
                     ERIGDRLKGLLKR"
     gene            9261..9779
                     /gene="grpE"
                     /locus_tag="VFR57_06520"
     CDS             9261..9779
                     /gene="grpE"
                     /locus_tag="VFR57_06520"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0259055.1"
                     /translation="MQEETVQPQSEPKSLEALLAEAQAKIEQQRDTMMRAVADAENAR
                     KRAQAEATNAQKYALERFAEGLLPVVDSLEAAVKSADTSGVELTLKQLKGALEKSSVR
                     EIDPKPGERFDPYRHQAMAAVPSEAEPNTIVAVMQKGYALHDRVLRPALVSVAKALEK
                     AAENPTSSTDSN"
     gene            9791..>10748
                     /locus_tag="VFR57_06525"
     CDS             9791..>10748
                     /locus_tag="VFR57_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU0259056.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGKPKVIENSEGARTTPSVVAYTD
                     DGEILVGAPAKRQAVTNAKNTVYAVKRLIGRRFEDKETQKDIKLMPFKIVRADNGDAW
                     VEVRGKKIAPQQVSAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQMKKDAKIAVYDLGGGTFDISIIEIADVEGEKQFE
                     VLSTNGDTFLGGEDFDQRLIDYIVTEFKKEQGVDLAKDVLALQRLKEAAEKAKIELSS
                     SQQTEVNLPYITADQSGPKHLNMKLTRAKFESLVDELIEKTIE"
BASE COUNT         1883 a         3391 c         3474 g         2000 t
ORIGIN      
        1 cgcgggtcgc aaggcgagga tgtgtgcatt gccgacatct gagctcgcgg cggcggtgga
       61 gcgcttggct ccggtcggcg cggccgttgc ctggagttga tatgtgcccg taatcgactg
      121 gccgatgccg cccaggccta tcgcacggtc gaaggcttcg gtgagggcgg cctcggcggt
      181 ccagctttgc gcggcggcgt agctatgcgc cgtgacaagc atggcattcg cggcgctggt
      241 tgtgatgctt ggcgtgtcgt ggctcgtgct gctcggcgtg ctgtgcccgc tttccgcatc
      301 aatcgggttg gcggtgtcga cgcgcgagaa cgactggatc ccacccacga tgaacgtacc
      361 acccgctatc cgccagacgt gcgtggcagg ctctcccggc gcggcggtcc tgctatatat
      421 cgcgagcacg ttcgaggttt gggcggcgtt attcatgcgt cgcacgttcg tccagccggc
      481 gggcggcgtc gcaaacgcac tggagtggtt gaacgcgatt gcagcgacca tgacgtcacc
      541 gggtacggtc cccgctggag tcgcgataga aagatttgcg cccgccggac gcagcgccag
      601 aagatgcgca ttgccaacat cggctttccc atcagcggtc gccgctttag agccggttga
      661 cccagccacg ctttggagcg ctcggttgac ctcaatcgat attcccccgg ggccgccctc
      721 gctccgtcgg tcgacagatt ccgtcatgcc gccgggcggg tcccacgtct ccgaggatgc
      781 aaaagcgtgc gccgacacca acatggtatt ggcgatagaa gtcgtgattg acggcgccgc
      841 atggatcgtc cccaatgggg tgttctgacc gttctccgcg tcaatcggat tcgcagtatc
      901 gatgccgcta aaagcttgga tgcctccgac gctgaagctg gcccccggta cgtcccaaac
      961 gtagctcgcc ggctctactg tcagcgccac tttgtggaag accgcaagtg aattcgacga
     1021 ggaaccgggg ttgtccaggc gccgcacgag ggtccaaccg gcaggcggcg taataacggc
     1081 gctcgaaggt cgcaccgcga ctgacgcaat gaggacatcg ccggccgcga cgccggcggg
     1141 cttgttgatc gagaggttgc ccgctctgct cgcggccgaa gcggcagcac ggaatgttgg
     1201 tgtgatgcct tgcgctgttg cagacgcagc gccgcgaaag ctgatttgtc cgtacgcccc
     1261 acctgcggca ataagcgtga ctactgtgag cgccgcggca atcagattgg cacaggtctt
     1321 catcgtggcg ccgcttgcct attggacgat gccgaccgga atatcgccga gatagattag
     1381 ttcgcgcttg accgctccgc ttgcgtcggt ttcggcgatc agccgtcctt gcgtgtcgta
     1441 gtggaagacc gtgtcgccga gggtgttcgt cttgcggatg cgctggccaa gcgcgttcac
     1501 ctggtagtcg gtgataccga taacgctcgc cgcgtgcacc atgcgccctc gcgcgtcgta
     1561 gccatatgtg ttgatgccgt ctgctgtggt ggagccgttt gggtcgaaga cgaagttcct
     1621 gactggtccg ctggatggcg tgatggacaa aattcggtta ctcgtcgcgc tataggcgta
     1681 ggtatggacg ctggctcccg ccaagcgcat cagccgattg cctattgcgt cgtactcata
     1741 gctgtacgct gtgcccggga cgagggcggc ggtaaggcgg tcaaggttgt cgtaatcgta
     1801 cgtattggtg ttcgccggat tgccggcctg ggcaatcgag cggatacggc tagcttggtc
     1861 gtagccaatg aggaagatct gcccgcccag cgtgtacgtc gcgatgcggc cgtcctgatc
     1921 gatgtcgcgg ctatagatct ggccatttcc aaaggtgtag cccttcacgc cgccgaatgg
     1981 gtggtaagcc acgcccgcga gaaccgtctg cggctgtcca ccggaccttg tcgtagtcac
     2041 gctgctcacg cggcctagcg gatcgaacgt gtaattcacc gtgcggccgg acgggtaagt
     2101 cagctgatcg agacggccga aggcgtcgta gcggtaggcc atgaagtgcg ctacgccggc
     2161 gaccgtacgt gtttccgagg tcacccgacc gtgcaggtca tacgtgtagt ggatggagct
     2221 cgtcacttga ttggcgtgat ttatctcggc gatcgacgac agccgcccgg cggcgttcgg
     2281 gccctggtcg tagccgaagc tatgtctcgc gccatcgtgg aacgtgataa gcaggacgcg
     2341 attgagcgcg tcataggcgt actgcgtggt ttgattcttt gcgtcggttt gaccaagcag
     2401 atttccggcg gcgtcgtaaa cgcttgccgt tgcgccggtg tccggactca attgctccac
     2461 gacattgcca agtccgtcca cgctgtagga agtcacgagg ctgcgtggat ccgtgacaga
     2521 agtgagtgcg tcgaggccgt tgtagccgta ctgcgtcacg gcaaagattg gcgccggcga
     2581 ggtgacttgt ttgagcctgt ggaaagggtc gtactcgttg agcgtgacgc gactgagcgg
     2641 atccttgacc gccagcacgt tgccctgatt gtcatagctg tactcggtgg tctggccttc
     2701 ggcccccaac tccctgaaaa ctcggttgag ggcattgatc acacggcttc gcgtctgcac
     2761 gagctcgctc gtcacgttgc gcacttcctc gagcgtgcga ttgcccatgg cgtcgagcgt
     2821 gtaggcgatg cggttgctga cgctatcttc tatgccggta agtcggtgcg cagcgtcgta
     2881 cctgtataaa aggaaagaac cgtctggcaa cgtcaccttg gtgagctgtc ccacgttgtc
     2941 gtagtcgtac gttgtcgact cgttgccgac ggtgcgcgcc ttcagtcgtt ggcgcgcgtc
     3001 gtagaacaaa gtagtggtga gtccgtttgc atcgacaatg gctagcggtt gcccatgcgc
     3061 gttgtatgcc gtgatgctcg tctggtggcc ggccgcgttc gtgaccgttt gaacattgcc
     3121 gcgcttgcct ggatcggagt cgtcgtttgc gtagtacctg tacgtcgtga cgtccggaac
     3181 atcggtgcga ggaccgtcga tggaaagcac aacgccgttg gcgttgtacg tgtacgccca
     3241 agtacgcggc ggcccgctgg gactagcaga gaagccggtc gagccgttcg tgtcggtggt
     3301 cgcctggatg ctcttggagc acagtactcc gcgcgcgccg cattggttgc cgtccgcgtc
     3361 gtagacgaag ctcgtcatcc tgagcggctc tgcgatgctg accggtagcc ggaagaccgc
     3421 gtgccaccgc gtggtggtcg tacgagtcag cggcgtcggg tcgccgctcg cggtcaatcc
     3481 ctcggtgcgc gaagtctcga ggttgcgcgt aaggtcatag gcgtagtccg tgcgattgcc
     3541 gttccaatca acccgggccg caacgttgcc gtttgcgtcg aaagtctggg aagcgggacc
     3601 gcactccggg cacacggcac cgctgatgcc ggtcgtcttg cgcacatcga ataaggttgt
     3661 aaagccgtac tcgcgcacgg cgcccaaagg cccggttaca gtggtggacc cgtcgccgta
     3721 tagaagtgca tgtctcatgg cgccgcccgc gtgctcggtt gttatcgcac gctgttgcgc
     3781 gtcatactga aaagtggcaa agcgatcgcc gttctcatcg acgatgccac tcagcgcatc
     3841 cggcaggtcg gcgccgcccg tgtgcggggg ctcgttatag aggtaagtcc gcgtaaccga
     3901 atccggaaac gtaatgctgg tgagtttgcc ggccacgtaa acaaaccggt acaccccgcc
     3961 tccgggatcg gtgagcttcg ttacccgcga agtgatgtcg tagccaaagg caagcacgcg
     4021 tccgaagtgg tcgctggcgc gaataagaag gccggcaggc agtaccgacg tggtcgcatt
     4081 gccgttggca tcgagcacga atccgccgtt cgggccgccg gtgccgtcgc tataggccat
     4141 tgtctgcgtg agtccgttcc tgcttcgaat ggcgaggagc cgccctgccg cgtcatatgt
     4201 ttcgaactcg tcagcgtttg ccgccgccag ctgccaaccg gttcgagtgc caggcgggtt
     4261 ctgcacctcg accagccggt cggccgtatc ggcatcggtg atccaggcgc cgccgtttgg
     4321 agtgaaccga agcgctcgcc catccggacg aaacgccacg gcagccgtcc gcaccagact
     4381 gatgcggcgg tcaaaggagt cgcgccagcg agtaccgaaa cgcagcccgg aaacatcgaa
     4441 gctgttgaag gagagcgcga gttcgaaccc ggctaggccc cgatacaccg attgctgctc
     4501 aaatttgttt cctgtcgctg gattgcaggg gttgcctacc gtcgacgtgc cgcgcgcact
     4561 ggggtcggta cacgagccgt tgttctttgc cagagcgaga ttcgcgacgc aactattgct
     4621 gttctccgcc tgctcgaatc cggggttgca gacgcagttc ccatcgtcgg tcactttcga
     4681 attggcgggg caaccgccaa tgaggcaggc gactccattg agggcgactg ttccgttgaa
     4741 ttcccgccac agacaattga ccgcttgtcc gatgttcgag aaacctggcg gaggttggca
     4801 accccaatgg atactgggag cagtaacacc aagaaacggt cggccctgct tcgcattgac
     4861 ggggcaagcg gggtttgttc cgtcctgtat taggcacgct tcctcggcgc ttccccggac
     4921 acaggggagc accacgaaac cgggcacgta ctgaaccgcc ggaaactctg tttgcgccac
     4981 accggacgtc gatgggactg ccaccactaa gccgacgaaa agtcttatga agcgcctcac
     5041 ggcgcgcaac tttcggcggc gctgcggttt cctccaaggc tcgaggtgat cgagaaccaa
     5101 acgactgcgt tgaactgcct cacgccaccc cctctcccgt tggcgttgcg cggtccctaa
     5161 ctgcttctcc gcggaccttg ctatacgacg ccgctataga ggcgcaaata gcgaaatgcc
     5221 gagacctttc aacgtcaggc tgtgcgtaaa cacacggcca atagttctag acctgcggtg
     5281 ctgttggccg cgcgagcttg ggccaaggac tcctggataa aaactgaaga gaccgtcgag
     5341 ctagtggacg ggttgctcgt cgccaaccgc cgcgcggcgg cttagcgtca acggatagga
     5401 gcggcgcagc gcctcgacga gcgcctcttc cggcagcggg cggctgaaga aaaagccctg
     5461 cagctcggtg caatagcgac cgcgcaggaa ttccacctgc cagggttcct ccacgccttc
     5521 ggcgatgagc ccgatgccga gcgagcgcgc gagcgccgaa atcgccttta cgatcgcggc
     5581 gtccttcggg ctcgtggcga tgtcgcgcac gaagctccgg tcgatcttca ggctgtcgat
     5641 cggaaagcgc ttgagatagc caagcgacga atagccggtg ccgaagtcgt cgatggagat
     5701 gaccgtgccg attgccttca gttgtcccat cacgcccatc gcgtgctcga cgttctcgat
     5761 gagcaggctc tcggtgagct cgagctccag gagctgcggc gcgagcccgc tcgagcgcaa
     5821 cgcctcgctc accgcgtcgc aaagcgcctc gctgcggaac tggcgcgatg agaggttgac
     5881 cgagacgcgc aggggcggca ggcccgcgct ctgccatgcg cgcgccgccc ggcacgcctc
     5941 ggcgagcacc caggcgccca ccggcacgat cagtcccgtc tcctcgagca gcggcacgaa
     6001 gcgcgccggc tcgaccaggc cccactccgg gtgcttccag cgaagcagcg cctcggcgcc
     6061 ggtcgcgcgg ccggtggcgg cggaaacgcg cggctgaaag taaagcacga actcctcgtt
     6121 cttgagcgcg cgccgcagcg cggcctcgac catcatctgc gtctcgacgc gctcgttcat
     6181 ctcccgggcg aagaacgcgt agcggttgcg tccgagctcc ttcgcgcggt acatcgcggt
     6241 atcggcggcc ttcaccaggc gctcgccgac gacgtcgtcg cgcggataaa gcgcgatgcc
     6301 gacgctcgag gtgaccgcga tctcgtggtg ctggagctgc agcggctccg cgatcgtttc
     6361 caggacgcgc gtcgctacca tcgccgcctc ctcgggcccg cgcacatcct cgagcagcac
     6421 ggtgaattcg tcgccgccca tgcggaacag catgtccgcc tcgcgcaccg cgcggcggat
     6481 gcgctcggcc gcgacgcgca gcaggcgatc gccggcgtca tggcccaggc agtcgttcac
     6541 gcgcttgaat agatcgagat cgaggaagag gagcgcgagc ggccagccgg cgcgcttcgc
     6601 gcgccgcatc gcctcctcca agtgctcggt gaagctcgcg cggttgggta gcccggtgag
     6661 cggatcgtga taagcctggt gccggatgcg ctcctcctgc gccgcgcgct cgcgctcctt
     6721 ctcctcgagg agccggcggt agcgcgcgag catgagctgc accacggcgt agagcagcgc
     6781 catcgcgccg agcaccgcgg ccagcacctt ccactcgctg cgctgattcg cctcgacgag
     6841 cctcgtcaca tcgctgtaga tctcgagcac cgcctcgacc ggagcggcct cgtgcacgtg
     6901 caccggcaca taggtggcaa ggatgtggcg ctgggcgatt tcgccttccc agctgtccat
     6961 gcgctcgcga taggtgaggt tgctcaccgg gtagccgtct cgcgcgcgcc gaaaaccggg
     7021 attcccggcg gcgctctgcc cgatctggcg cgggtcggtc gagtagacgg tcaggccgtg
     7081 caggtcgtag atcttgacct tgacgatgtc gaggccgctc gccaggcggc gcaggtcgcc
     7141 gtcgagcacg ccgatctcgg cacggctggc gagcgcgggc cgctcgagcg cgcccgcgcg
     7201 cctcacgaac gccgcgtgcg caggccagat cgcgttggcg aaggtcctgg tgagcgcgac
     7261 gttggcatgc gtctcgccct cgaccatcga atcgaagacg aggccgcggt agaggtaggt
     7321 cagcgcgacc accaccgcga tgggcacggc cagcgcgatc agctgtgagt accgcaggag
     7381 attgaacatc gcgcgggcta gcctacgggc gcgcgcgccg agcggcactc ggtgcttccc
     7441 ctaaggggaa acccctaccg ctttaacaaa cccttaagcc gatcgccgat gcgttcctcg
     7501 attttgtcct tgcggctctc gatctgttcc ttgacgcgct cgccgacctg cgatttcgcc
     7561 gcgttgcccg ccatggcgcg caggtcgacg tcgtatttca agtcgtcgaa gctgccgtgc
     7621 aagcgtaccg gcaccgtgag acccgcgagc ttgtcgcgct cgcgtccgcc ctgcccttcg
     7681 gcggtcgcga cgatagccgc cttcaccaca tagttgagcg tcgactccgg aatgttgatg
     7741 tcgccggagc cgccgaggcg aaagagcggc gccttggcgt ctaggtcttc gttgtgcgca
     7801 atgccgttct tgatcgtaaa gctcgcggag agctcgctga aatcggtctg cggcttgccg
     7861 ctgcctccgc tctcggcttg cgcggcctgc gagccgccgc cgagcgtcga gcgcgctttg
     7921 cgcaggattt cgcccaagtt gatgcccttc actgcgcctt cgcgcaattg cacccgcgcc
     7981 gtgccgccga gcgcgcgctt catcgcgttc accgtgttgc cggcggtcgt gacgtcgagc
     8041 gtcagattgc ctttgccctc gagccggtcc tgctgcgcga agtcgcgcag aatcggcccg
     8101 atcgagatgc cttcgagctt ttccttcagc gtgacgtgat tcttgttggc atccacgatc
     8161 acttcacccg aaatcgtgcc ctcgtagccc ctcgccgagt gcggggcgac cgccacgcgg
     8221 ccgttcgcgg cgttcagctg cgcgttcaac tcggaaagct tcagaccgcg cacttcgaac
     8281 gccccgacct gcagtttgcc tttcgcgttg atgcccttca gcgccgagaa atcgaccggt
     8341 gcgtcttcgc tcttcgccgt ctgctgcggc gccggtttcg ccgctggctt ctcaggcgcg
     8401 ccgaggtagc ggtcgagatt gatcttgtcg atgctcgcct cgaactcgta gggctcggcg
     8461 agcgtgaact tcgccttgag cgtcgactcg tcgagcttgc cgttcacatc ggcgctcagc
     8521 gtgccgttgg tgaaccgata gcggccgtcg agcgcaaggc gcgcctcgag cggcggggct
     8581 ttgcgcttcg ccagcatgcc gagctcgagc ttgccctcgg catcgcttgc gatgcggccg
     8641 gtcttgattc tcacgtcttc gagcatgtac tcctgcccgg cctgcaggtc gctgtacgcg
     8701 atggaggcgc gctggatgtc aatcgagccg atgtcgaaca ccacagcgcc gccgcccttt
     8761 ttgggctcct gcttcggcgc gggcttcttc gcgtcggcct cgacgagatc cgagaagttg
     8821 tagcgtccgt ccttgccctt gacgatgcgc gccttgaagc cggaaagctg caccgagtcg
     8881 acgatgtact gaccgcgcag gagcggcatc agcttcaccg aggcgtgcgc ggagtcgaag
     8941 gccagaaatt gctggtttga ggcgcgttcc gagagcgtga cgccggagac atcggcgccg
     9001 aggctcggcc agaacgccat cttgatgtcg cccttgatgg cgagcgtgcg cccggtctgc
     9061 tccttggcga ggcgctcgat ctcgtccttg tagcggttcg ggtcgaacgt gagcgcgaag
     9121 accagcgccg cgccgatgac aaggacgatg acaagcccta ggccgagcag cgtgtatttg
     9181 acaggtttat tcataaaatc aacaccttta aattaccgcg ggcggcttga aagcggcgcc
     9241 gtcgtcccca attcgacaag atgcaggaag aaaccgttca gccccaatcc gaaccgaagt
     9301 ccctcgaggc gctgctcgcc gaagcgcagg cgaaaatcga gcagcagcgc gacaccatga
     9361 tgcgcgccgt ggcggacgcc gagaacgcgc gcaagcgcgc gcaggccgaa gccaccaacg
     9421 cgcagaagta tgcgctcgaa cgcttcgccg aggggctgct gcccgtcgtc gacagcctcg
     9481 aagcggcagt gaaaagcgcc gatacgagcg gcgtggagct cacgctcaag cagctcaagg
     9541 gcgccttgga gaagtcgagc gtgcgcgaga tcgacccgaa gccgggcgag cgcttcgatc
     9601 cctaccgcca tcaggccatg gctgccgtgc cctccgaggc cgagcccaac accatcgtag
     9661 ccgtgatgca gaagggctac gcgctccacg accgcgtgct gcgccccgcg ctcgtcagcg
     9721 tcgccaaggc ccttgaaaaa gcggcggaaa accccacctc tagcacagat tcgaactgat
     9781 cgggaaaccc atggcaaaaa tcatcggcat cgaccttggc acgacgaact cctgcgtcgc
     9841 aatcatggag ggcggcaagc ccaaggtgat tgagaactcg gagggcgcgc gtacgacccc
     9901 gtcggtcgtc gcctataccg acgacggcga aatcctggtc ggcgcgccgg ccaagcgcca
     9961 ggcggtcacc aacgcgaaga acaccgtcta cgcggtgaag cgcctgatcg ggcggcgctt
    10021 tgaggacaag gaaactcaga aggacatcaa gctgatgccc ttcaagatcg tgcgcgccga
    10081 caatggcgac gcgtgggtcg aggtgcgcgg caagaagatc gccccgcagc aggtatcggc
    10141 cgaagtcctg cgcaagatga agaagaccgc cgaggactac ctcggcgagg aggtgaagga
    10201 agccgtcatc acggtgcccg cgtacttcaa cgattctcag cgccaggcca ccaaggatgc
    10261 cggccgcatc gcggggctcg atgtgaagcg catcatcaac gagccgaccg cggccgcgct
    10321 cgcattcggc ctcgacaagc agatgaagaa ggacgccaag atcgcggtct atgacctcgg
    10381 cggcggcacg ttcgatatct cgatcatcga gatcgccgac gtcgagggcg agaagcagtt
    10441 cgaagtgctc tccaccaacg gcgacacctt cctcggcggc gaggacttcg accagcgcct
    10501 gatcgactac atcgttaccg agttcaagaa ggaacagggc gtcgatctcg ccaaggacgt
    10561 gctcgcgctc cagcggctga aggaagccgc ggagaaggcg aagatcgagc tttcgtcctc
    10621 gcagcagacc gaggtcaacc tgccgtacat cacggccgac cagagcgggc cgaagcacct
    10681 caacatgaag ctcacccgcg cgaagttcga gtcgctggtg gacgagctca tcgagaagac
    10741 gatcgagc
//
LOCUS       DASTPD010000197         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_98904, whole genome shotgun sequence.
ACCESSION   DASTPD010000197 DASTPD010000000
VERSION     DASTPD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98904"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFR57_06530"
     CDS             <1..714
                     /locus_tag="VFR57_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0259057.1"
                     /translation="QLEEASRHKSQFLASMSHELRTPLNAILGFNEMILGQVYGEVPS
                     DMKEPLEDIQKSGKHLLRLINNVLDLAKIEAGKMELSLADYSVHDTVESVRSTLRPLA
                     EAKGLELVVSVPEDIPLAYGDGGRLTQCLMNLAGNSLKFTKEGKVEIAVQAENGCLRW
                     RVSDTGIGIPADKIPTLFSEFKQTDATIASEYGGTGLGLSITKKFVEMHGGRIWVESE
                     LGKGSVFTLEIPLRVTAPA"
     gene            711..1085
                     /locus_tag="VFR57_06535"
     CDS             711..1085
                     /locus_tag="VFR57_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0259058.1"
                     /translation="MSAKTILYIEDNEMNRKIVRDLLKRTKYALIEAHDGEAGVAKAL
                     EARPDLILMDIQLPKISGLEATKRLRAEAATAKTPIIAITSFALSGDDVKAKEAGATA
                     YLAKPYSPFDLLKLIRTILPET"
     gene            1082..>2964
                     /locus_tag="VFR57_06540"
     CDS             1082..>2964
                     /locus_tag="VFR57_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0259059.1"
                     /translation="MSERTRKRATKGRKRKTATPGTLQRRLDEALAREAASAEILEVI
                     ASSPSNVQPVFDAIVRSAVRLCGSLFGAVYRFDGKKLHFVAHHNFPPAALKIVRGDYP
                     KPPDCSQGSGRAILTKSVVCIEDIRRDRDYHRAFGQRGGWRRVLAVPMMRSGTPLGTI
                     SVAWTDPGPIPGSQVALLKTFADQAVIAIENVRLFNELKTTNSSLREALEQQTATSEI
                     LGVISSSPTDVRPVFKTIGERARLLCQADVSVISHFDGELLQLAELHVAEEGAEAVRA
                     VFPMRTDAATVTARTFREREIVHLEDVLAERSYTAYKEIARMARWRGVLGVPMMREGQ
                     VTGVIFVGRERPGLFSGSQVALLKAFADQAVIAIENVRLFNQTKEALEQQTATAEILR
                     VMSSSPSDVQPVFDAVARKTSELCSGNYAIVVRFDGELIHLAAQHNARPGADQPTAQR
                     YPRRPGRETPSSRAILERAVVHIPYADKDPDLSQDLVRGIRAASFLSVPLLRESRPIG
                     AIGVSRIESGPFPDKQIELVKLFADQAVIAIENVRLFNETKEALEQQTASAGILRVIS
                     STPSDTRPVFEAIVHAGLRVFAGAGVAVVMADADRVRVVAAAGVFGPMATNVEMPRNR
                     ESAS"
BASE COUNT          572 a          960 c          964 g          468 t
ORIGIN      
        1 cagctcgagg aggcgagccg gcacaagtcg cagttcctcg ccagcatgag ccacgagctg
       61 cgcacgccgc tcaacgccat cctcggcttc aacgagatga tcctcggcca ggtttacggc
      121 gaggtgccga gcgacatgaa ggagccgctg gaagacatcc agaagagcgg caagcatctc
      181 ctgcggctca tcaataacgt gctcgacctt gccaagatcg aggcgggcaa aatggagctc
      241 tcgctcgccg actactcggt gcacgacacg gtcgagagcg tgcgctcgac cctgcgtccc
      301 ctggcggagg cgaaggggct cgagctcgtc gtgagcgttc ccgaggacat tccgctcgcc
      361 tacggcgacg gcgggcggct cacgcagtgc ctcatgaacc tcgccggtaa ttcgctcaag
      421 ttcaccaaag agggcaaggt ggaaatcgcg gtgcaggcgg agaacggctg cctcagatgg
      481 cgcgtatccg acaccggcat cggcatcccg gccgacaaga tcccgacgct cttctccgag
      541 ttcaagcaga ccgacgccac tatcgcgagc gaatacggcg gcaccgggct cggcctttcc
      601 atcaccaaga aatttgtcga gatgcacggc gggcggatct gggtagaaag cgagctcggc
      661 aagggttcgg tctttaccct tgaaatcccg ctcagggtga cggcgcccgc atgagcgcga
      721 agaccatcct ctacatcgaa gacaacgaga tgaaccgcaa gatcgtgcgc gacctcctga
      781 aacgcacgaa atatgcgctc atcgaggcgc acgatggcga ggcaggggtc gcaaaagcgc
      841 tcgaggcgcg cccggacctg attctgatgg acatccagct tccgaagatc tcgggccttg
      901 aagcgacgaa gcgcctgcgc gccgaggcgg ctaccgccaa gacgcccatt atcgcgatca
      961 cctcgttcgc gctctcgggg gacgacgtga aggcgaagga ggccggcgcc accgcctacc
     1021 ttgccaaacc ctatagcccg ttcgacctcc tcaagctgat ccgcacgatt ctgccggaga
     1081 catgagcgag cgcacccgca aacgcgcaac aaaggggcgc aagcgcaaga cggccacgcc
     1141 cggcacgctg cagcgccggc tcgacgaggc gctcgcgcgc gaagcggcga gcgccgagat
     1201 cctcgaagtc attgcgagct cgccttcgaa cgtccagccg gtgttcgatg cgatcgtgcg
     1261 cagcgcggtg cggctctgcg gcagcctgtt cggcgcggtc tatcgcttcg acggcaagaa
     1321 actgcacttc gttgcgcatc acaatttccc gccggccgct ctgaagatcg tgagaggcga
     1381 ctatccgaag cctccggatt gcagtcaggg tagcgggcgc gcaattctca ctaagtccgt
     1441 cgtgtgcatc gaggacatcc ggcgcgaccg cgactatcac cgggcattcg gccagcgggg
     1501 cggctggcgc cgggttctgg cggtgccgat gatgcggtcg ggaacgccgc tcggcacgat
     1561 cagcgtcgcc tggacagatc cggggccgat tccaggcagc caagtcgcgc tcctgaagac
     1621 cttcgccgac caggcggtaa tcgcgatcga gaacgtgcgc ctgttcaacg agctgaaaac
     1681 gactaacagc agcctgcgtg aggcgctcga gcagcagact gcgaccagcg agatccttgg
     1741 ggtgatcagc agctcgccga ccgacgtgcg gccggtgttc aagaccatcg gcgagagggc
     1801 gcggctcttg tgccaggccg acgtcagcgt catttcgcac ttcgatggcg agttgctgca
     1861 gctcgcggag cttcacgtcg ccgaggaagg ggcagaggcg gtgcgggccg tgtttcccat
     1921 gcgcacggac gccgccacgg tcactgcgcg cactttccgg gagcgcgaga tcgtccacct
     1981 cgaagacgtt ctcgccgaac ggagctacac cgcctacaaa gaaatcgcgc gcatggcccg
     2041 ctggcgtggc gtgcttggcg tcccgatgat gcgcgaaggg caggtcaccg gcgtgatctt
     2101 cgtcgggcgg gagcggccgg ggctgttctc cggctcgcaa gtcgcgctgc tcaaggcgtt
     2161 cgccgaccaa gcggtcatcg caatcgagaa cgtacgcctc ttcaaccaga ccaaggaggc
     2221 gctcgagcag cagaccgcca cggctgagat tctgcgcgtc atgtcgagct cgccaagcga
     2281 cgtgcagccg gtgttcgacg ccgtcgcgcg caagacgagc gagctttgca gcgggaacta
     2341 cgcgatcgtc gtgcggttcg acggcgagtt gatccatttg gctgcgcagc acaacgcacg
     2401 gcccggcgcc gaccagccga ccgctcagcg ttacccacgg cgtcccgggc gcgaaacgcc
     2461 ttcctcacgc gcgatccttg aacgagccgt agtgcatatt ccatacgcgg acaaagaccc
     2521 ggatctctcg caggatctcg tccgcggcat acgagctgca agcttcctgt cggtcccgct
     2581 gctgcgcgaa agccggccga tcggcgcgat cggtgtctcg cgcatcgaat ccggaccttt
     2641 ccccgacaag caaatcgagc tcgtgaagct gttcgccgac caggcggtga tcgcgatcga
     2701 gaacgtgcgc cttttcaacg agaccaagga ggcacttgag cagcagacgg cgagcgcagg
     2761 catcctgcgc gtcatctcca gtactccgag cgatacgcgg ccggtgttcg aggcaatagt
     2821 gcacgcgggg ttgcgcgtat ttgccggcgc cggcgtcgcg gtcgtgatgg cggacgccga
     2881 ccgcgtgcgt gtagtagcgg cagcgggagt gttcgggccc atggctacga acgtcgagat
     2941 gccgcgcaac cgcgagagcg cctc
//
LOCUS       DASTPD010000198        10924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1733708, whole genome shotgun sequence.
ACCESSION   DASTPD010000198 DASTPD010000000
VERSION     DASTPD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733708"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3804
                     /locus_tag="VFR57_06545"
     CDS             <1..3804
                     /locus_tag="VFR57_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU0259060.1"
                     /translation="NIADVTTAVAKGDLSKKITVDVRGEILELKNTVNTMVDQLNAFA
                     AEVTRVAREVGTEGKLGGQAQVRGVGGVWKDLTDNVNSMAANLTGQVRNIADVTTAVA
                     KGDLSKKITVDVRGEILELKSTINTMVDQLNAFASEVSRVAREVGTEGKLGGQAQVRG
                     VGGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVARGDLSKKITVDVRGEILALKNTFN
                     TMVDQLNGVASEVSRVAREVGTEGKLGGQAQVTGVAGVWKDLTDNVNSMAANLTGQVR
                     NIAEVTTAVAKGDLSKKITVDVRGEILDLKSTFNTMVDQLNAFASEVSRVAREVGTEG
                     KLGGQAQVVGVAGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVARGDLSKKVTVDVKG
                     EILELKNTVNTMVDQLNAFASEVSRVAREVGAEGKLGGQAQVRGVGGVWKDLTDNVNS
                     MAANLTSQVRSVASVVTAVAHGDLNRKLTVEAKGEVAALADIINGMIDTLATFADQVT
                     NVAREVGSEGKLGGQARVPGAAGLWRDLTENVNRLAANLTSQVRAIAEVATAVTKGDL
                     TRSITVEASGEVASLKDNINEMIHNLRETTRKNTEQDWLKTHLARFTRMLQGHRDLVT
                     VSRLILSELTPLVNAQQAAFYVADHRSGDSQLELLAGYAQRQGKSLPRKIAFGEGLVG
                     QCAFEKKRIVLSQAPADYIRIGSSLGAGAPVSIIILPVLFEGQVKAVIELASFAKFSE
                     IHQTFLDQLTESIGVVLHTIAANMQTEYLLHQSQSLTAELQSQQEELKKTNDRLEQQA
                     VNLQKSEALLKAKQDELRSANEQLQEKARQLSDQMAQVEFKNQEIEQARSALEEKAEQ
                     LSLSSRYKSEFLANMSHELRTPLNSLLILARLLADNVDGNLTPKQVEFARTIHSSGAE
                     LLALINDILDLAKIESGTVTLNIAAERFDEVTDSVERTFRQVATDKGLEFNITVSPEL
                     PASLQTDGKRLQQILTNLLSNAFKFTAKGEVRLRMEPATSGWSLGHPVLDRARPAVAF
                     SVVDTGIGIPSNKQRLIFEAFQQADGSTSREYGGTGLGLSISRELARLLGGEIRVASV
                     PGEGSTFTLYLPLDYQRAGEPAEEAEGVSIEQQPPPVADEQAEVLAPQASEALTGRRV
                     LVVDDDIRNVFAMTSALEMHGMRVLHAESGKEGIETLKRERGIDLVLMDVMMPGLDGL
                     DTMRIVRSLDGYRQVPIICVTAKAMMGDREKCLEAGASDYIAKPVNVDVLLATLWRAL
                     QPTPA"
     gene            3801..4067
                     /locus_tag="VFR57_06550"
     CDS             3801..4067
                     /locus_tag="VFR57_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259061.1"
                     /translation="MNREFDSLLETLAGLAAANIVMIETLHEQGVVDKTHFAHAFARA
                     LDTLAPEHRGGMIEQVLRNMRDRCMNLPSGQGSDAREWLRQILS"
     gene            complement(4064..4303)
                     /locus_tag="VFR57_06555"
     CDS             complement(4064..4303)
                     /locus_tag="VFR57_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259062.1"
                     /translation="MPIKPNGALCAIKEQIVEDLVSGLILQFECQNGRTRLVIAGSAL
                     PFGNREIIFDSEGREAAAGTLVGEFRRPSWLRKVT"
     gene            4436..4744
                     /locus_tag="VFR57_06560"
     CDS             4436..4744
                     /locus_tag="VFR57_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259063.1"
                     /translation="MPIASPRYALTAVMVSGAPNDPGVYALWENDELIYYGHARGESV
                     TIQSCLKEHLSRQNACTAGATHYGWEISANPPPREADLLREFERQHKRLPRCNGAKRA
                     "
     gene            4770..5084
                     /locus_tag="VFR57_06565"
     CDS             4770..5084
                     /locus_tag="VFR57_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259064.1"
                     /translation="MSIRFQSLPFTQPLIDAAPDTLGVFALWQNGGVIYLGKASAGAA
                     TIRKALAEQFQARRWSSSAPLRCSWEVADDPDQRHSELLREFEEAHRCAPLWNDPQRL
                     PT"
     gene            5161..7038
                     /locus_tag="VFR57_06570"
     CDS             5161..7038
                     /locus_tag="VFR57_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0259065.1"
                     /translation="MRSVVDHVVDGIITIDARGTIATFNAAAERIFGYHSAETIGKNV
                     KMLMPEPYHSGHDGYIANYLNTGRAKIIGTGREVIGRRKDGSDFPMELAISEFRLENH
                     RYFTGIVRDITQRKQAEAELRDAEERMRSVVNHVVDGIITIDERGKIESFNPAAEKLF
                     GYKRADVMGRNVKVLMPEPYHSEHDGYIANYVGSGHAKIIGIGREVTGKRQDGSTFPM
                     ELAVSEFHIGTRRFFTGIVRDITERKRLQEELQQRLRDLADADRQKNEFLAMLSHELR
                     NPLAPMRNALYLLKNTSQGNGDLSAVHAMMERQLQQLVRLVDDLLDVSRIIRGKIDLR
                     KEEVDLVHAVHRAAETAKPVIDAHGHALHISVPEEPILVTGDLVRLAQVIANLLTNAA
                     KYSRATAPLELAVARAGDDAVVRVKDHGVGIPASLLPRIFDLFVQGEHALARSQGGLG
                     IGLTLVKRLVEMHDGTVSVLSDGDGRGSEFRIRLPALARRAAPASAAVPVPGGGRAHR
                     VLVVDDNVDAAESIAKILRLCGHEVRCEYDGQSALAAVRDYTPDVVVLDIGLPGMDGY
                     EVASRLRSMDRRSMRIVAVTGYGQEEDRRRSRESGFDQHLTKPVDPDALQAFVSGAST
                     G"
     gene            complement(7080..7154)
                     /locus_tag="VFR57_06575"
     tRNA            complement(7080..7154)
                     /locus_tag="VFR57_06575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7120..7122),aa:Val,seq:cac)
     gene            7190..8254
                     /locus_tag="VFR57_06580"
     CDS             7190..8254
                     /locus_tag="VFR57_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0259066.1"
                     /translation="MIFRQLFDPQSSTYTYLLADPASREAVLIDPVFEQARRDAALID
                     ELGLKLAWTIETHVHADHVTGAWLLREKLGSRIALSRSSNAEGADHYLEPGERLAFGK
                     RYLEARATPGHTSGCTTYVLDDHSMAFTGDALFIRGCGRTDFQGGDARRLYRSVREQI
                     FSLPDRCLIYPGHDYRGLTVTSVGEEKLYNPRLAESIGEGDFVGYMTNLGLPHPKQMD
                     VAVPANLKCGKPQTTTSLDPDWAPLTYTFAGIWEVQPHWLEEHLKDVQIVDVREPEEY
                     NGPLGHVPGAKLVPLGKLNERAAELKKAQPVVVVCRSGARSANATLMLGKAGFDKVAN
                     LSGGMLRWRAQRLAVEGGTD"
     gene            8251..9639
                     /gene="gltX"
                     /locus_tag="VFR57_06585"
     CDS             8251..9639
                     /gene="gltX"
                     /locus_tag="VFR57_06585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0259067.1"
                     /translation="MKVRTRFAPSPTGYLHVGGARTALFSWAYARRHGGAFVLRIEDT
                     DLERSTKESVDAILHGLQWLGIEWDEGPFFQMQRLARYKEVAAQLIASGHAYYDYMSR
                     EDLEQLRREQLQRGEKPRYDGRWRPERARGRKIPEGVKPVVRFRTPETGEVGWKDLVK
                     GVVSFPNAELDDLVLLRADGVPTYNFGVVVDDIDMDITHVIRGDDHVNNTPRQIHIFE
                     ALKASLPHFAHVPMILGADGERLSKRHGAVSVLQYRDEGYLPEALGNYLARLGWSHGD
                     EEIFSSRQFIGWFDLEHVSRSAAQFNPEKLAWLNQQYIKNAPDARLAALVEPELRTRG
                     VQPGGGPNLERVIRLVKDRANSIPQLADEAMLFYVYELKVRPSDFGADALKALRTLKA
                     RLAAVPWERAAINDTIKDVAKSSGLKMPQVAMPLRHIVTGRTQTPSIDAVLELLGREM
                     VLTRLAQHLETD"
     gene            9640..9984
                     /locus_tag="VFR57_06590"
     CDS             9640..9984
                     /locus_tag="VFR57_06590"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU0259068.1"
                     /translation="MDIDPGQWHLEATTQLRGIREPSVAVRERCLTAEDVANPLRLFG
                     TSIGRRCTFSNPRDTGSVITFEVVCNSKQPMKGSGELRYRRDAFEGELQAKGETFEAR
                     SKVKGRRTGECN"
     gene            9975..10394
                     /locus_tag="VFR57_06595"
     CDS             9975..10394
                     /locus_tag="VFR57_06595"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU0259069.1"
                     /translation="MQLRATPLLVVLLLAMPAFAAEVDPGEWEFTATSTSRLFPGPQM
                     TSFKRCIKKDDAANPDRWMAQQTRQGDCKVTPGAKSADTYTWVMECAKPSMRGTGSAR
                     LSRASIEGETHMTGEVHGQKFELRTKVSGKRLGPCKG"
     gene            10431..10506
                     /locus_tag="VFR57_06600"
     tRNA            10431..10506
                     /locus_tag="VFR57_06600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10464..10466,aa:Ala,seq:ggc)
     gene            10529..10604
                     /locus_tag="VFR57_06605"
     tRNA            10529..10604
                     /locus_tag="VFR57_06605"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10563..10565,aa:Glu,seq:ttc)
BASE COUNT         2093 a         3547 c         3575 g         1709 t
ORIGIN      
        1 aacatcgccg acgtcaccac cgcggtggcg aagggcgacc tctccaagaa gatcacggtg
       61 gacgtgcgcg gcgagatcct cgagctcaag aacaccgtca acacgatggt ggaccagctg
      121 aacgccttcg ccgcggaagt aacgcgggtc gcccgcgaag tcggtaccga aggcaagctc
      181 ggcggccagg ctcaggtgcg gggggtgggc ggcgtatgga aggaccttac cgacaacgtg
      241 aactcgatgg ccgcgaacct caccggtcag gtgcgcaaca tcgccgacgt caccaccgcg
      301 gtggcgaagg gcgacctctc gaagaagatc acggtcgacg tgaggggcga aattctcgag
      361 ctgaaaagca ccatcaacac gatggtggac cagctgaacg cgttcgcctc cgaggtgtcg
      421 cgcgtagcgc gcgaggtcgg cacggaagga aagctcggcg gccaggcgca ggtgcgcggc
      481 gtgggcggcg tgtggaagga cctcaccgac aacgtgaact cgatggccgc gaacctcacc
      541 ggccaggtgc gcaacatcgc ggaagtgacc accgcggtcg cgcgcggcga tctttccaag
      601 aagatcaccg tcgacgtgcg cggcgagatc cttgcgctca aaaacacgtt caacacgatg
      661 gtggatcagc tgaacggcgt cgcctccgaa gtgtcgcgcg tggcgcgcga ggtgggcacc
      721 gagggcaagc tcggcgggca ggcgcaggtg accggcgtgg ccggcgtctg gaaggacctg
      781 accgacaacg tgaattcgat ggccgcgaac ctcaccggcc aggtgcgcaa catcgccgag
      841 gtcaccacgg cggtggcaaa gggggatctc tccaagaaga tcacggtgga cgtgcgtggc
      901 gagatcctcg acctgaaaag cacgttcaat acgatggtgg accagctgaa cgccttcgcc
      961 tccgaggtgt cgcgcgtcgc gcgcgaagtg ggcaccgagg gcaaactcgg cggccaggcg
     1021 caggtcgtcg gcgtggcggg cgtctggaag gacctcaccg acaacgtgaa ctcgatggcg
     1081 gcgaacctta ccggccaggt gcgcaacatc gccgaggtta cgaccgcggt cgcgcgcggc
     1141 gacctttcga agaaggtcac cgtggacgtg aagggcgaga tcctcgagct caagaacacc
     1201 gtgaacacca tggtggacca gctgaacgcg ttcgcgtccg aagtgtcgcg cgtggcgcgc
     1261 gaggtgggcg cggaaggcaa gctcggcggg caggcgcagg tacgcggcgt cggcggcgtg
     1321 tggaaggacc tcaccgacaa cgtgaactcg atggccgcca acctgacgag ccaggtgcga
     1381 agcgtggcaa gcgtggtgac cgcggttgcc cacggtgatc tcaaccgcaa gctcacggtc
     1441 gaggcgaagg gcgaagtggc ggcgctcgcc gacatcatca acggcatgat cgacacgctc
     1501 gccacgttcg ccgaccaggt gacgaacgtg gcgcgcgagg tgggaagcga aggcaagctc
     1561 ggcgggcagg cgcgtgtgcc gggcgccgca ggcctgtggc gcgatctgac ggaaaacgta
     1621 aaccggctcg ccgcgaacct caccagccag gtgcgcgcga tcgcggaggt cgcaaccgcg
     1681 gtgaccaagg gcgacctgac gcgttcgatt acggtggaag cctcgggcga ggtggcctcg
     1741 ctcaaggaca acatcaacga gatgattcac aatttgcgcg agaccacgcg caagaacacc
     1801 gagcaggact ggctgaagac ccacctcgcg cggttcacgc gcatgctgca gggtcacaga
     1861 gacctggtca ccgtttctcg attgatcttg agtgagctaa cccctctggt taacgcgcag
     1921 caggccgcgt tctacgttgc ggatcaccgc agcggcgatt cgcagctcga gctcctcgcc
     1981 ggctacgcgc agcgccaggg caagagcctg ccgcgcaaga tcgccttcgg cgagggcctg
     2041 gtcggccagt gcgcgttcga gaaaaagcgc atcgtgcttt cgcaggcgcc ggcggactac
     2101 atccgcatcg gttcgtcgct cggcgccggg gcgccggttt cgatcatcat cttgccggtg
     2161 ctgttcgaag ggcaggtgaa agcggtgatc gagctcgcct cgttcgcgaa gttcagcgag
     2221 atccaccaga ccttcctcga tcagctcacc gagtcgatcg gcgtggtgct gcataccatc
     2281 gcggcgaaca tgcagaccga atacctgctg caccagtcgc agtccctcac cgccgagctg
     2341 cagagccagc aggaagagct gaagaaaacg aatgatcgcc tcgagcagca ggcggtcaac
     2401 ctgcagaaat ccgaggcgct cctcaaggcg aagcaggacg agcttcgctc cgccaacgag
     2461 cagctgcagg agaaagcgcg ccagctctcc gaccagatgg cgcaggtcga gttcaagaac
     2521 caggaaatcg agcaggcgcg ctcggcgctc gaggagaaag cggagcagct ctcgctttcc
     2581 tcgcgctaca agtcggagtt cctcgcgaac atgtcgcacg agctgcgcac gccgctcaac
     2641 tcgcttctca tcctcgcgcg gctcctcgcc gacaatgtgg acggcaacct cacgccgaag
     2701 caggtcgagt tcgcgcgcac gatccattcg tcgggcgccg agctgctcgc gctcatcaac
     2761 gacatcctcg acctcgccaa gatcgagtcg ggcacggtga cgctgaacat cgctgccgag
     2821 cgcttcgacg aggtgaccga ttcggtggag cgcactttcc gccaggtggc gaccgacaag
     2881 gggctggaat tcaacattac ggtgagcccg gagctgcccg cctctctgca gaccgacggc
     2941 aagcgcctgc agcagattct caccaacctg ctctcgaacg cgttcaagtt caccgccaag
     3001 ggcgaggtca ggctgcgcat ggagccggcg acctcgggct ggagcctcgg acatccggtg
     3061 ctcgatcgcg ccaggccggc cgtagcgttc tcggttgtcg acacggggat cggcattccg
     3121 tcgaacaagc agcgcctcat tttcgaggcg ttccagcagg ccgacggctc gacgagccgc
     3181 gaatacggcg gcaccgggct cggcctttcc atcagccgcg agcttgcacg cctgctcggc
     3241 ggggagatcc gcgtggcgag cgtgccgggc gagggcagca ccttcacgct ctacctgccg
     3301 ctcgactatc agcgcgcggg tgaaccggcc gaggaagccg aaggcgtcag catcgagcag
     3361 cagcctccgc cggtcgccga cgagcaggcg gaagtgctgg cgccgcaggc gagcgaagcg
     3421 ctcaccggcc gccgcgtgct ggtcgtcgac gacgacatcc gcaacgtgtt cgcgatgacg
     3481 agcgcgctcg agatgcacgg tatgcgcgtg ctgcacgccg agagcggcaa ggaaggcatc
     3541 gagaccctca agcgcgagcg cggcattgat ctggtgctga tggacgtcat gatgccgggc
     3601 ctcgacggcc tcgacaccat gcgcatcgtg cgcagcctcg acggctacag gcaagtcccg
     3661 atcatctgcg tgaccgcgaa ggcgatgatg ggtgaccgcg agaaatgcct cgaggccggc
     3721 gcaagcgact acatcgccaa gccggtcaac gtggatgtgc tgctcgccac gctctggcgc
     3781 gcgctgcagc cgacgcccgc gtgaatcggg agttcgattc gctgctcgag acgctcgccg
     3841 gcctcgccgc ggcgaacatc gtcatgatcg agacgctgca cgagcagggc gtggtcgaca
     3901 agacgcactt cgcgcacgcc ttcgcgcgcg cgcttgatac gctcgcgccg gagcaccgcg
     3961 gcggaatgat cgagcaggtg ctgcgcaaca tgcgcgaccg ctgcatgaac ctgccgtcgg
     4021 gccagggctc cgacgcgcgc gagtggttgc ggcagatctt gagctaagtc accttgcgca
     4081 gccaggaggg ccggcggaac tcgccgacca gcgtgcccgc ggccgcttcg cgtccctcgc
     4141 tgtcgaaaat gatttcgcgg ttgccgaacg gcagcgcgct gcccgcgatg acaaggcgcg
     4201 tgcggccgtt ctggcattcg aactgcagaa tcaatccgct caccaggtct tcgacgatct
     4261 gttccttgat cgcacacaag gctccgttgg gtttgatcgg catgcagagc gtccagcaac
     4321 acgcataccc gctgcagtgt ggcgcgacgg cgacgttgtt ggcgaacgcg ctgcttaggg
     4381 aaacgcccga cagagagtcc gtgagccttc atgtaagctc gatcgcagga aaaaaatgcc
     4441 gatcgcaagc cctcgctacg cacttaccgc cgtgatggtg tccggtgcgc ccaacgatcc
     4501 gggcgtttat gcactgtggg aaaacgacga gcttatctac tacggccacg cccgcggcga
     4561 aagcgtcacc attcagtcgt gcctgaagga gcatctctcg cggcagaacg cctgcaccgc
     4621 gggcgccacg cactatggat gggagatctc cgcgaacccg cccccgcgcg aggccgatct
     4681 gctgcgcgaa ttcgaacgcc agcacaagcg attgccgcgc tgcaacgggg caaaacgggc
     4741 gtaacatttt tcctcgcaga gggggaaaca tgtccatccg attccagagc ctgccgttca
     4801 cgcaaccgct gatcgacgcg gcgcccgata cgctcggtgt gttcgcgttg tggcagaacg
     4861 gtggagtgat ctacctcggc aaggcctccg ccggcgcggc gacgatccgc aaggcgcttg
     4921 ccgaacagtt ccaggcgcgc cgctggtcga gcagcgcgcc cttgcgctgc agctgggaag
     4981 tggccgacga cccggaccag cgccacagcg agctcctgcg cgagttcgag gaagcgcacc
     5041 gctgcgcgcc gctgtggaac gatccgcagc gcttaccgac ttaaaaaatc ggggtcagag
     5101 cccgattctt tggcgttgga gcgtttccac agcgcgccga cctcgccatc cgaagagcgg
     5161 atgcgctccg tcgtcgacca tgtggtcgac gggatcatta ccatcgacgc tcgaggaacc
     5221 atcgcaacgt tcaacgccgc cgccgagcgc atcttcggct accacagcgc cgagactatc
     5281 ggcaagaacg tgaaaatgct gatgccggag ccgtaccact cgggacacga cggctacatc
     5341 gccaattacc tgaacaccgg ccgcgcgaag atcatcggca ccgggcgcga ggtgatcggc
     5401 cggcgcaagg acgggagcga tttcccgatg gagcttgcca tcagcgagtt ccgcctcgag
     5461 aaccatcggt atttcaccgg catcgtgcgc gacatcacgc agcgcaagca ggccgaggcc
     5521 gagctgcgcg acgccgaaga gcgtatgcgc tcggttgtga accacgtagt ggacggcatc
     5581 atcaccattg acgagcgcgg caagattgag tccttcaatc cggcggcgga gaagctcttc
     5641 ggctacaaac gcgccgacgt catgggccgc aatgtcaagg tgctgatgcc cgagccgtac
     5701 cactcggagc acgacggcta catcgcgaat tacgtcggca gcggtcacgc caagatcatc
     5761 ggaatcggcc gagaggtgac gggcaagcgc caggacggat cgaccttccc gatggagctt
     5821 gcagtgagcg aattccacat cggcacgcgg cgcttcttca cgggcatcgt gcgcgacatc
     5881 actgagcgca agcgcctgca ggaagagctt cagcagcggc tgcgcgacct ggcagacgcc
     5941 gaccggcaga agaacgagtt cctcgccatg ctctcgcacg agctgcgcaa tccgctcgcg
     6001 ccgatgcgca acgccctcta cctgctcaag aacacgagcc aagggaatgg cgatctgtcg
     6061 gcagtccacg ccatgatgga gcgccagctc cagcagctcg tccgccttgt cgacgatctg
     6121 ctcgacgtct cgcgcatcat tcgcggcaag attgatctgc gcaaggaaga ggtcgacctc
     6181 gtgcacgccg tgcaccgcgc ggcagagacc gcgaagccgg tgatcgacgc ccacggccac
     6241 gcgctccaca tctccgtccc cgaggagccg atactggtca ccggcgacct ggtgcggctc
     6301 gcgcaggtga tcgccaatct gctcacgaat gcggcgaagt actccagggc caccgcgccg
     6361 ctcgagctcg cggtggcgcg cgcgggcgac gacgccgtgg tgcgtgtcaa ggatcacggg
     6421 gtcggcatac cggcgtcgct tctcccgcgc atattcgacc tcttcgtgca aggcgagcat
     6481 gccctcgcgc gctcgcaggg cggcctgggc atcggcctca cgctcgtcaa gcggctggtc
     6541 gagatgcatg acggcacggt ctcggtcttg agcgatggcg acggccgcgg cagcgaattc
     6601 cgcatccgcc tccccgcgct ggctcggcgg gctgcgccgg cgagcgccgc agtgcctgtc
     6661 ccgggcggcg gccgcgcgca ccgcgtactc gtcgtggacg acaatgtcga tgcggccgag
     6721 agcatcgcca agatcctgcg gctctgcggt cacgaggtgc gctgcgagta cgacggccag
     6781 tcggcgcttg ccgccgtgcg cgactacacg cccgacgtgg tcgtcctcga catcggtctg
     6841 ccgggaatgg acggctacga ggtcgcgagc cggctgcgct cgatggatcg acgtagcatg
     6901 cgcattgtcg cggtcacggg atacggtcag gaagaggatc ggcgccgctc gcgggaatcg
     6961 ggattcgacc agcacctcac caagccggtc gaccccgacg cgctgcaggc cttcgtgagc
     7021 ggcgcgagca ctggttgatt tggtattctt ggagagccgt gccggtcact ggacgattct
     7081 ggtgggtgct agagggttcg aacctctggc cccttccgtg tgaaggaagt gctctaccgc
     7141 tgagctaagc acccgatccg gcgacgggaa gaattctagc aaaggcccga tgatttttcg
     7201 gcagctcttc gatccccaat cttcgacgta cacctatctt ctcgcggatc cggcctcgcg
     7261 cgaggcggtg ttgatcgacc cggtgttcga gcaggcgcgg cgcgacgcgg cgctcatcga
     7321 cgagctcggc ctcaagctcg cgtggaccat cgagacacac gtgcacgccg accacgtgac
     7381 cggtgcctgg ttgctgcgcg agaagctcgg cagccgcatc gcgctttctc gctcctccaa
     7441 cgccgaaggc gcggaccatt atctcgaacc gggcgagcgc ctcgcattcg gcaagcgcta
     7501 cctcgaggcg cgcgcgacac ccgggcacac cagcggctgc acgacctacg tgctcgacga
     7561 ccactcgatg gccttcaccg gcgacgcgct cttcatccgc ggctgcggcc gcaccgactt
     7621 ccagggcggc gatgcgcgcc ggctctatcg cagcgtgcgc gagcagatct tctcgctgcc
     7681 cgaccgctgc ctgatctacc ccggccacga ctaccgcggc cttacggtga cgagcgtcgg
     7741 cgaggagaag ctctacaacc cgcgcctcgc cgaatcgatc ggcgaaggcg atttcgtcgg
     7801 ctacatgacg aacctcgggc tgccgcaccc caagcagatg gacgttgccg taccggcgaa
     7861 cctgaagtgc ggaaagccgc agaccaccac ttcgctcgac cccgactggg caccgctcac
     7921 ctatacgttc gccgggatct gggaggtgca gccgcactgg ctcgaggagc acctgaaaga
     7981 cgtgcagatc gtcgatgtgc gcgagcccga ggaatacaac ggcccgctcg gccacgtccc
     8041 cggggcgaag ctggtgccgc taggcaagct gaacgaacgc gctgcggagc tgaaaaaggc
     8101 tcagcccgtc gtggtggtct gccgttccgg agcccgctcg gcgaacgcca ctctcatgct
     8161 cggaaaggcg ggtttcgaca aggtcgcgaa cctttccggc ggcatgctgc gctggcgcgc
     8221 gcagcgcctt gcggtcgagg gcggcaccga ctgaaggtcc gtacccggtt cgcgccgagt
     8281 cccaccggct acctgcacgt cggcggggcg cgtaccgcgc tcttctcctg ggcgtacgcg
     8341 cgccgccacg gcggcgcctt cgtgctgcgc atcgaggaca ccgacctcga gcgctcgacg
     8401 aaagagtcgg tcgacgcgat cctgcacggc ttgcaatggc tcggcatcga atgggacgag
     8461 gggccgttct tccagatgca gcgccttgcg cgctacaagg aagtcgccgc ccagctcatc
     8521 gcttcggggc acgcctacta cgactacatg tcgcgcgagg acctcgagca actgcggcgc
     8581 gagcagctgc aaagaggcga aaagccgcgc tatgacggcc gctggcgccc cgagcgcgcg
     8641 agaggccgca agatccccga gggcgtgaag ccggtggtgc gctttcgcac cccggaaacc
     8701 ggcgaggtcg gttggaagga tctcgtgaag ggcgtggtga gctttcccaa cgccgagctt
     8761 gacgatctgg ttctcttgcg cgcggacggc gtgccgacct acaacttcgg cgtggtggtc
     8821 gatgacatcg acatggacat cacgcatgtc attcgcggcg acgaccacgt caacaacaca
     8881 cctcgccaaa tccacatctt cgaggcgctg aaagcaagcc tgccgcactt cgcgcatgtc
     8941 cccatgattc ttggcgccga cggcgagcgc ctctccaaac ggcacggcgc ggtaagcgtg
     9001 ctccagtatc gcgacgaggg ctatctgccg gaagcgctcg gaaactacct cgcgaggctc
     9061 ggctggtcgc acggcgacga ggaaattttt tcgtcccggc agttcatcgg gtggttcgat
     9121 ctggagcacg tgagccgctc cgccgcgcag ttcaaccccg aaaagctcgc ctggctgaac
     9181 cagcagtaca tcaagaacgc ccccgacgcg cggcttgcgg cgctcgtcga gccggagctg
     9241 cgcacgcgcg gcgttcagcc cggcggcggg cctaacctgg agcgcgtgat ccggcttgtc
     9301 aaggaccggg cgaactcgat cccgcagctc gccgacgagg caatgctctt ctatgtgtat
     9361 gagctcaagg tgcggccaag cgactttggc gccgacgcgc tcaaggcgct gcgcacgcta
     9421 aaagcgcgcc tcgcggctgt gccgtgggag cgcgccgcga tcaacgacac gatcaaggac
     9481 gtggcgaagt cgagcggcct caagatgccg caggtggcga tgccgctgcg ccacatcgtt
     9541 accgggcgca cgcagacgcc ctccatcgac gccgtgctcg agcttctcgg gcgcgaaatg
     9601 gtgctgacac gactcgcgca gcacctggag accgattgaa tggatatcga tcccggccag
     9661 tggcacctcg aagcgaccac ccagctgcgc ggcatccgtg aaccgtcggt ggccgtgcgc
     9721 gagcgctgcc tcactgccga agatgtcgcg aatccactgc ggcttttcgg cacctccatc
     9781 gggcggcgct gcacgttctc caacccgcgt gatacgggta gcgtcattac gttcgaagtg
     9841 gtgtgcaatt cgaagcaacc gatgaagggc tcgggggaac tacgctaccg ccgcgatgcg
     9901 ttcgaaggcg agctccaggc gaagggtgaa acattcgagg cccgctcgaa ggtcaagggc
     9961 cgccgaacag gggaatgcaa ctgagagcga ctccgcttct cgtcgtccta ctgcttgcca
    10021 tgccggcgtt cgcggcggaa gtcgatccgg gcgaatggga attcaccgcc acgtcgacct
    10081 cgcgcctctt tcccggtccg caaatgacaa gcttcaaacg ctgcatcaag aaggacgacg
    10141 ccgcgaatcc tgatcgctgg atggcgcagc agacccggca gggagattgc aaagtcacgc
    10201 ccggcgcgaa gagcgccgac acctacacct gggttatgga atgtgcgaaa cccagcatgc
    10261 gcggcacggg ctccgcgcgc ctgagccgcg cgtcgatcga aggcgagact cacatgaccg
    10321 gcgaggtgca cggccagaaa ttcgagctgc gcaccaaggt gagcggcaag cgcctcggcc
    10381 cgtgcaaggg gtgatccgga tttgatcggc tataatttcg ccccttggtt gggggtatag
    10441 ctcagctggg agagcgcttg catggcatgc aagaggtcac cggttcgatc ccggttacct
    10501 ccaccagcca accttgttga agtccgcagt ccccatcgtc tagaggccta ggacatcgcc
    10561 ctttcacggc ggtaaccggg gttcgaatcc ccgtggggac gccaaacctt tataaacggg
    10621 gtccaacaag gggccaaggg tagtccggcc ctcaaacctc agggtccgtc ggccttggac
    10681 cgtggcaggc gcgatttgca aaggttcggc caatagccgc cgcaaagctt ggcgagctaa
    10741 cggcacatcg ctcaatagca gttcctcgaa gcgtttcagt tgggtccctc aacgcctgat
    10801 cgaaggcgca gggcttaagc gcggccgagt aactggccca attacgatgg tgatcgcgga
    10861 tgactcttct ctccgagtgt cctggatgga cagtaaaaac ttgcgaatcg aagaacgcgt
    10921 cctt
//
LOCUS       DASTPD010000199         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1530009, whole genome shotgun sequence.
ACCESSION   DASTPD010000199 DASTPD010000000
VERSION     DASTPD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530009"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFR57_06610"
     CDS             <1..432
                     /locus_tag="VFR57_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259070.1"
                     /translation="GYDHAAFETLDLDAVGLAAEWLQRQGWIKSWGVGRHILGSQIFC
                     YHYDPSGFAVEHYADGDVFDAEAPTRYHDGGVAGLYAWGPDLPKHFIDTGMSPRRLAK
                     VVRGLRTREEFTLARLLAAKRTYSGQPRPWAGRRFRKPEPA"
     gene            complement(479..1432)
                     /locus_tag="VFR57_06615"
     CDS             complement(479..1432)
                     /locus_tag="VFR57_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259071.1"
                     /translation="MKPVYALLFALFAATTAYAQQDRPVRFILPNAAGSGVDAITRAA
                     QPAFAKAFAAPVVVENQPGAGGVVGLQALARSAPDGNTLSVVSNNVVIFPSVLKSLPF
                     EIPGDFTPIALVGSTPMVLVVNPSRVAATNHKELAALVKSKPDALNYASSGNGTILHL
                     AVEIYLEAAGLKVKHIPYKGVGPMVTDLLGGQVDFAAAALPSVQGHIASGKLRPIGVF
                     PAQRTPAAPDIPTFAEQGLKNFEVEAWFAVIGPKGMSPATVKKVHDAIVSAFNDAAVK
                     EAMAKQGNTIRITSTEQAQAAFRTELAKYAALVKKVGLEPQ"
     gene            1472..1714
                     /locus_tag="VFR57_06620"
     CDS             1472..1714
                     /locus_tag="VFR57_06620"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEU0259072.1"
                     /translation="MPGSGAHRGAAWGARAVARRSGKPPSVGDIAYYAPWGNLAMFYT
                     DAPYARGLLPLGRIDSSIVALRASGTSKVTIERAPK"
     gene            1756..3045
                     /locus_tag="VFR57_06625"
     CDS             1756..3045
                     /locus_tag="VFR57_06625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0259073.1"
                     /translation="MFLRAEQTGLRLAIIGRIAALALMGVWLISTRADDPTRMIGYIL
                     VLSLFAALGLAHYALIGTRFDRPWVKYVFITLDVAILSTLVATQPLYQSAAGLPAVMT
                     FRAPVFPFYFVILGVAAFSFSPAMVMWTGIAGAIGWLLAFGHSASMVDGVRNWSEIPN
                     NPTAEQIMAIVLDPKFGGLSGRIQEAVLLAVVAFLIAVVMWRARSTLRRQLEAERERA
                     TLSGMFGRFVPQAIVTAMVAGRGALAPVEREATVLFADIAGFTAMTQRAGAARTVEIL
                     NAFFEEVTRIIGAHNGIVTQFQGDGVLATFNVPVEDPNHASNAFQAAEEILACVACRD
                     YAGERIRVRIGINTGSLVAGNVGGGGRQSYTVHGDAVNLAARLEALCKEHGTSLLLSA
                     TTAKALPEAQLVAVGEIAVRGFAQAATVYSIPAASGR"
BASE COUNT          536 a         1110 c         1073 g          589 t
ORIGIN      
        1 ggttacgatc acgccgcttt cgagacgctc gacctcgacg ccgtgggact cgccgccgaa
       61 tggctgcagc gccagggctg gatcaagtcc tggggcgtcg gacggcacat cctcggctcg
      121 cagatcttct gctatcacta cgacccgagc ggattcgcgg tggagcatta cgccgacggc
      181 gacgtgttcg acgccgaagc gccgacgcgc taccacgatg gcggcgtcgc cggtctctac
      241 gcctggggac cggatctgcc gaagcacttc atcgacaccg gcatgtcgcc gcggcgcctg
      301 gcgaaagtgg tgcgcggact gcgcacgcgc gaagagttca ccctcgcgcg cctgctcgcc
      361 gccaagcgca cctacagcgg gcagccccgg ccgtgggccg gccgccgctt ccgcaagccc
      421 gagccggctt gatgcgcccc gttcgcctaa aaacgtaacg gccgttcttg accggtcgtt
      481 actgcggctc cagccccacc tttttcacca gagcggcgta cttcgccaac tcggtgcgga
      541 acgctgcctg cgcctgctcc gtgcttgtga tgcggatcgt gttgccctgc ttcgccatcg
      601 cttccttcac cgccgcatcg ttaaacgcgg aaacgattgc gtcgtgcacc tttttcaccg
      661 tcgccggact catgcccttc gggccgatca cggcgaacca tgcctcgact tcgaagttct
      721 tcagcccctg ctcggcgaag gtcggaatgt cgggcgccgc aggcgtgcgc tgcgccggaa
      781 atacgccgat cgggcgtagc ttgccgctcg cgatatggcc ctgcacgctc ggcagcgcgg
      841 ccgcggcgaa atcaacctgc ccgcccaaga ggtcggtgac catcggtccg acgcccttgt
      901 acgggatgtg cttgaccttg aggcccgcgg cttcgaggta gatttccacg gcgaggtgca
      961 ggatggtgcc gtttccgctc gaagcgtagt tgagcgcatc cggcttcgac ttgaccagcg
     1021 cggcgagctc cttgtgattc gtcgccgcca ccctggatgg gttcacgacg agcaccatcg
     1081 gcgtggagcc gacgagcgcg atcggcgtga agtcgcccgg tatctcgaac ggcagcgatt
     1141 tcagcacgct cggaaagatc accacgttat tggagaccac cgagagcgtg ttgccgtcgg
     1201 gcgcggagcg cgcgagcgcc tgcagtccca ccacgcctcc tgcaccgggc tggttctcca
     1261 ctacgaccgg agccgcgaag gccttagcga atgccggctg cgccgctcga gtgatggcat
     1321 cgacgccgga gcctgcagca ttcggcagga tgaagcgcac cggacgatcc tgctgggcgt
     1381 acgcagtggt cgcggcgaat agggcgaata gaagcgcgta gactggtttc atggcggaca
     1441 ttctaaggtt cgagcgtcgc gtactgtttc tttgccagga tccggcgctc atcgaggcgc
     1501 agcttggggg gcgcgagctg tcgcgcggcg aagcgggaaa cctccgtcgg tcggcgacat
     1561 tgcttactac gctccgtggg gcaatctggc gatgttctac acggatgccc cgtatgcgag
     1621 agggctcctg ccattgggtc ggatcgattc ctcgatcgta gctctgcgcg cgtcaggtac
     1681 gtcgaaggtg acaatcgaac gggcgccgaa ataaagaccc ccgccgcagc gcaggcctct
     1741 ggcggcgccg aggagatgtt cctgcgcgcc gagcagaccg ggctcaggct cgcgatcatc
     1801 ggacgcatcg ctgcgctcgc cctgatgggc gtctggctga tcagcacgcg cgcggacgac
     1861 ccgacgcgga tgatcggcta catactggtc ctgtcgctct tcgccgcgct cggcctcgcg
     1921 cattacgcgc tgatcggcac ccgcttcgac cggccttggg tcaagtacgt cttcatcacg
     1981 cttgacgttg ccatcctctc gacgctcgtg gcgacgcaac cgctctacca gtccgcggcc
     2041 ggcctgcctg cggtgatgac gttccgcgcg cccgtgttcc cgttctactt cgtcattctc
     2101 ggcgtggcgg cgttcagctt ctcacccgcc atggtgatgt ggaccggcat cgcaggtgcg
     2161 atcggctggc ttctcgcttt cgggcactcg gcgagcatgg tcgatggcgt gcgcaactgg
     2221 agcgagatcc cgaacaaccc gaccgccgag cagatcatgg cgatcgtgct ggatcctaaa
     2281 ttcggcggcc tgagtggccg tatccaggag gcggtcctcc tcgccgtagt ggcttttctg
     2341 atcgcggtcg tcatgtggcg ggcgcgcagc acgttgcgac ggcagctcga agcagaaaga
     2401 gagcgcgcca cgctgtccgg gatgttcggg cgcttcgtgc ctcaggcgat cgtgaccgcc
     2461 atggtcgccg ggcgcggcgc cctcgccccg gtcgagcggg aagcgaccgt gctcttcgcc
     2521 gacatcgccg gctttactgc gatgacacag cgggccggcg ctgcgcgcac ggtcgaaatc
     2581 ctcaacgcct ttttcgaaga ggtgacgcga atcatcggcg cgcacaacgg catcgtcacc
     2641 cagttccagg gcgatggcgt gctggcgacg ttcaacgtgc cggtcgagga cccgaatcat
     2701 gcaagcaacg ccttccaggc tgccgaggag atcctggcgt gcgtcgcgtg ccgcgactac
     2761 gccggcgagc gcatccgcgt gcgcatcggc atcaataccg gctcactggt cgccggcaac
     2821 gtcggcggcg gcggacgcca gagctacacc gtccacggag acgcggtgaa cctggccgcc
     2881 cggctcgagg ctctgtgcaa agaacacggc acgtcgctgc ttctttccgc caccaccgca
     2941 aaagcgctgc ccgaggcgca gctcgttgcc gtcggcgaga tcgccgtacg gggcttcgca
     3001 caggcggcga ccgtctattc catacccgcc gcgagcggac ggtaaacgct gagggtacca
     3061 ctggggatac cagctcccgc gtgctcgtcc atatcgtcca acggcggcca ctacggccga
     3121 aaatcgattc ccgccgcctc caccatttca acgggctgtc agggactagt gctgccgccg
     3181 gatttggtcg accacgccgc gggccttctg acacaggacg aacgaccgag cgcgaaggta
     3241 catggcgagg gtgcgagcgg caaagctgcg cctgcgtagg tccctcaact gcgcttcaag
     3301 tgcggacg
//
LOCUS       DASTPD010000200         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1122240, whole genome shotgun sequence.
ACCESSION   DASTPD010000200 DASTPD010000000
VERSION     DASTPD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122240"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VFR57_06630"
     CDS             complement(<1..1152)
                     /locus_tag="VFR57_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEU0259074.1"
                     /translation="MPSVAEQWKIHASEYYAPKNFNFWYFFGSLALLVLVIQIISGIF
                     LTMNYKPDASLAFGSVEYIMRDVPGGWLIRYIHSTGASAFFIVVYLHMFRAMLYGSYR
                     EPRELVWIFGVLIYLTLMGEAFFGYLLPWGQMSYWGAQVIVNLFGTVPFIGPDLALWI
                     RGDYVISDATLNRFFALHVIALPLVLLGLVVAHILALHDVGSNNPDGVEIKKLKGPDG
                     KPLDGIPFHPYYTVKDLVGAVVFLAIFCVVMFFVPEMGGYFLEHNNFIPADPLKTPEH
                     IAPVWYFTPYYSILRAVPPLFNSQFPGVLAMGVAVVILFFLPWLDRSPVKSIRYRGPL
                     YKSWLAAFVIVFIVLGYLGIEPTTVWGQFPVSDDSFLGRTIVDTKDVATW"
     gene            complement(1202..1801)
                     /gene="petA"
                     /locus_tag="VFR57_06635"
     CDS             complement(1202..1801)
                     /gene="petA"
                     /locus_tag="VFR57_06635"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU0259075.1"
                     /translation="MNEEPKLDKTRRNLVVATSVVGGAASVAAAIPFVASMWPSERAK
                     AAGAPVEADISRIAPGELAVVEWRGKPVWILRRTKEMVESLKAVAPRLTDPASKSSEQ
                     PKYAENEYRSAKPELMVMEGVCTHLGCSPQLKSAEAKAEMGGDWVGGFYCPCHGSKFD
                     FAGRVFRGAPAPTNLKVPPYTFVSDNTLVIGEDQETKGA"
     gene            1927..3096
                     /locus_tag="VFR57_06640"
     CDS             1927..3096
                     /locus_tag="VFR57_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0259076.1"
                     /translation="MYRLWLIFAQAATVAVAVLFVVSTFHPEWLPSRAVTVVQQTPIA
                     ETTGPLPSAVRAGSFHEAVQRAAPSVVNISTSKEIRSPRHPLLNDPLFRRFFGSQLPD
                     DAAAQRASNLGSGVVVNAGGYVLTNHHVVEGADEIEVALHDGKKLLARVVGSDPETDL
                     AVLRVSADNLPAISFGSSDALRVGDVVLAIGNPFNLGHTVTSGIVSALGRSGLGVSTF
                     ENFIQTDAAINPGNSGGALIDAGGNLVGINTLIISQTGSAGGIGFAIPVSTAKMVLEQ
                     IIKTGGVTRGWIGVELRDVTPLDAASLQLAGTRGALIALVQRGGPADKAGVRPGDVAV
                     EVQGKPVADATAMLNVVAALAPGEPAKMKLKRQGQDVETTVNVGRRPRPQPRPAD"
     gene            complement(3118..4281)
                     /locus_tag="VFR57_06645"
     CDS             complement(3118..4281)
                     /locus_tag="VFR57_06645"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF005262.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU0259077.1"
                     /translation="MTIRIAAAAALAALLAASNAFAQLLTEKKFFTLPQYTTAGGKTI
                     KNVRVGYETYGKLNATGDNAVFIPHFFSGTSHAAGRYKPDEKAAGYWDAIIGSGKAID
                     TDKYFVVSADTLVNLNVKSPMVGTVGPATVNPDTGKPYGSSFPVVSMKDSVRVHKALL
                     DSLGVKRLQAVAGASGGAIQTMEWAVEYPQLVERVVHVIGPGLDVHPYTIAMLDAWTM
                     PIKLDSGWKGGDYYGGAEPVEGLAQALKLVTLTTVHYSWAEKLHGYKWAEEGKNPADS
                     MANLFSIEDALTKSGRSRAAANDAGHFVWMVKANALYNVDKDASRIKAKVLFLPATTD
                     LLFPPHYSRKAAEKLRAQGNHVELYEIDGTNGHLDGIFSIGKVADRIKAFLEK"
     gene            complement(4306..4932)
                     /gene="coq7"
                     /locus_tag="VFR57_06650"
     CDS             complement(4306..4932)
                     /gene="coq7"
                     /locus_tag="VFR57_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEU0259078.1"
                     /translation="MISVDRLIGEFDRALRAVAGVAHATRPSPAAGVSEKEITDEDRR
                     HAAALMRVNHVGEVCAQALYQGQALTARDAVARQALEQAAREEEDHLAWSVERIRELG
                     GRPSLLNPLWYAGAFAIGAVAGALGDRWNLAFLAETERQVEEHLGGHLEELPPEDDRT
                     RAVVEAMQADEARHRASAIELGAAELPEPVKRAMRLASKVMTTVAYRV"
     gene            complement(4929..>5432)
                     /locus_tag="VFR57_06655"
     CDS             complement(4929..>5432)
                     /locus_tag="VFR57_06655"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0259079.1"
                     /translation="GLVKRGRMGKGGSLVVDPEKKDSFDVSVDLVPAELLSEYGIERY
                     YAELQRCLQLYVEQHPMLKQLGKFRVTESPAIQHYRPGGGFKMPHFERSGYATATRML
                     VWMTYLNDVTDGGGTHFVYQKHTFEAKKGRTLIWPSDFTHTHVGVVSPNQHKYIITGW
                     MNFCPDA"
BASE COUNT          947 a         1779 c         1744 g          962 t
ORIGIN      
        1 ccaagtcgcc acatccttgg tgtcgacgat cgtgcggcca aggaacgagt cgtcgcttac
       61 cggaaactgc ccccacaccg tggtgggctc gatgccgagg tagccgagga cgatgaacac
      121 gatgacgaac gcggcgagcc agctcttgta gaggggcccg cggtagcgga tcgacttcac
      181 agggctgcgg tcgagccacg gcaggaagaa gagaatcacc accgcgacgc ccatggcgag
      241 cacgcccggg aactgcgagt tgaagagcgg cggcacggca cgcaggatcg agtagtaagg
      301 cgtgaagtac cacaccggcg cgatgtgctc cggagtcttc agcgggtcgg ccgggatgaa
      361 gttgttgtgc tcgaggaagt agccgcccat ctcgggcacg aagaacatca ccacgcagaa
      421 gatcgcgagg aacaccaccg cgccgaccag gtccttcacc gtgtagtacg ggtgaaacgg
      481 gatgccgtcg agcggcttgc cgtcgggccc cttgagcttc ttgatctcga cgccgtcggg
      541 gttgttcgag cccacgtcgt gcagcgctag gatgtgcgcg accaccaagc cgagcagcac
      601 gagcggcagc gcgatcacgt ggagcgcgaa gaatcggttg agcgtcgcgt ccgagatgac
      661 atagtcgccg cggatccaga gcgcgaggtc cggcccgatg aagggcaccg tgccgaacag
      721 gttcacgatc acctgcgcgc cccagtacga catctgtccc cacggcagca ggtagccgaa
      781 gaacgcctcg cccatcagcg tgaggtagat cagcacgccg aagatccaca ccagctcgcg
      841 tggctcgcgg tacgagccgt acagcatggc gcggaacatg tgcaggtaca cgacgatgaa
      901 aaatgcggag gcgccggtgg aatggatgta gcggatcaac cacccgcccg gcacgtcgcg
      961 catgatgtac tcgaccgagc cgaacgcgag gctcgcgtcg ggcttgtagt tcatcgtgag
     1021 gaagatgccc gagatgatct ggatcacgag cacgagcagc gcgagcgagc cgaagaaata
     1081 ccagaagttg aaattcttcg gcgcgtagta ctcggaagcg tggatcttcc actgctccgc
     1141 gaccgaaggc atgcgcgcgt cgaaccagcc ccagagcgcc tgaagacgcg atgccatcgc
     1201 cctatgcccc cttggtttcc tggtcttcgc cgatcacgag cgtgttgtcg ctgacgaagg
     1261 tgtacggcgg caccttgagg ttggtgggcg cgggcgcgcc gcggaacacg cggccggcga
     1321 agtcgaactt cgagccgtgg cacggacagt agaacccgcc cacccagtcg ccgcccatct
     1381 cggctttggc ctccgcgctc ttgagttgcg gcgagcagcc gagatgcgta caaacccctt
     1441 ccatcaccat gagctcgggc ttggccgagc ggtactcgtt ctcggcgtac ttcggctgct
     1501 cggaagactt cgacgccgga tcggtcagcc gcggcgcgac ggccttcagc gactcgacca
     1561 tttccttggt gcggcggagg atccacaccg gcttgccgcg ccattcgacc accgccagct
     1621 cacccggcgc gatgcgggaa atgtccgctt ctaccggggc ccccgccgcc ttggcgcgct
     1681 cggagggcca catgctcgcg acaaacggga ttgcggcggc cacgctcgcg gctccgccga
     1741 cgaccgacgt cgcgaccacc agattgcgcc gcgtcttgtc tagttttggt tcttcattca
     1801 tggagatggc ggtgttatgg aggcgcgcaa aagcctgcga ttctagcgac cgcccctgca
     1861 aaagtccacg atttcgctcc taaacgattg gatttgctag ggaaatctgc tttactacga
     1921 tcccccatgt accgcctctg gctcattttt gcgcaggccg ccaccgtcgc cgttgcggtg
     1981 ctcttcgtgg tatcgacctt ccatccggag tggttaccct cgcgcgcggt tactgtcgtg
     2041 cagcaaacac cgatagcgga aacgacgggc ccgcttccga gcgcggtgcg cgcggggtcg
     2101 tttcatgaag cggtgcagcg cgcggcgccc tcggtcgtca atatctccac ttccaaggag
     2161 atccgctcac cgcgccaccc gctcctcaac gacccgctgt tccgccggtt cttcggcagt
     2221 cagctgcccg atgatgccgc cgcgcagcgc gcctccaatc tcggctcggg cgtagtggtg
     2281 aacgccggtg gctacgtgct caccaaccat cacgtggtcg aaggcgccga cgagatcgag
     2341 gttgcgctgc atgacggcaa gaagctgctg gcccgcgtag tcggcagcga tcccgaaacc
     2401 gatctcgcag tgctgcgcgt aagcgccgac aacctgccgg cgatcagttt cggctcctcc
     2461 gatgcgctgc gcgtcggcga cgtggtgctc gccatcggta acccgttcaa tctcggccac
     2521 acggtgacga gcgggatcgt gtccgcgctc gggcgttcag ggctcggagt gtcgactttc
     2581 gagaatttca tccagaccga cgcagcgatc aaccccggca actccggcgg cgcactgatc
     2641 gacgccggcg gcaacctcgt cggcatcaac acgctgatca tttcgcagac gggcagcgcg
     2701 ggcgggatag gcttcgcgat cccggtgtcg accgcgaaga tggtgctcga gcagatcatc
     2761 aagaccggcg gcgtgacgcg aggctggatc ggcgtcgagc tgcgcgatgt cacgccgctc
     2821 gacgccgcct cgttgcagct cgccggcacg cgcggcgcgc tgatcgcgct cgtgcagcgc
     2881 ggcggcccgg ccgacaaggc gggtgtgcgg ccaggcgacg tggcggtgga ggttcagggc
     2941 aagccggtcg ccgacgccac ggccatgctc aacgtcgtgg ccgcgctcgc ccccggcgag
     3001 ccggccaaga tgaagctcaa gcgccagggc caggacgtcg agaccacagt caacgtcggc
     3061 cgccggccgc gcccgcaacc gcgccccgcg gactaagaaa tcggggtcag agcccgatta
     3121 tttttccaag aaagccttga tgcggtcggc gaccttgcca atcgagaaaa tgccgtcgag
     3181 gtggccgttg gtgccgtcga tttcatagag ctcgacatga ttgccttgcg cgcggagctt
     3241 ctccgccgcc ttgcgtgagt aatggggcgg aaatagcagg tcggtcgttg ccgggaggaa
     3301 gaggactttg gccttgatcc ggctggcatc cttgtcgacg ttgtagagag cgttcgcctt
     3361 caccatccac acgaagtggc ccgcgtcatt tgccgccgcg cgcgatctgc cggatttcgt
     3421 cagtgcgtcc tcgatcgaaa acaggttcgc catggagtct gcggggttct tcccctcctc
     3481 tgcccacttg tatccgtgga gcttctccgc ccacgagtaa tgcaccgtgg tcagggtgac
     3541 gagcttgagc gcctgcgcga gcccctccac cggttctgcg cctccgtagt agtcgccgcc
     3601 tttccagccc gagtcgagct ttatgggcat ggtccacgca tccagcatgg cgatggtgta
     3661 ggggtgcaca tccaggccag ggccgatgac gtggaccact cgctcgacga gttgcggata
     3721 ttccaccgcc cactccatcg tctggatcgc gccgcccgac gcgcctgcca ccgcttgaag
     3781 ccttttcacg ccgagcgagt cgagcaaggc cttgtgaacg cgcaccgagt ccttcatcga
     3841 tacgaccggg aagctggagc catagggctt gccggtgtcc gggttgacgg tggccgggcc
     3901 gacggtaccg accatcgggc ttttcacatt gaggtttacg agcgtatcgg cactgacgac
     3961 gaaatacttg tcggtgtcga tcgccttgcc cgagccgatg atggcgtccc agtagcccgc
     4021 cgccttctca tcgggcttgt agcgtccggc ggcatgcgaa gtgccggaga agaaatgcgg
     4081 aatgaacacc gcgttgtcgc ccgtggcgtt cagcttgccg tacgtctcgt agccgacgcg
     4141 cacgttcttg atcgtcttgc cgcctgcggt cgtgtactga ggcagggtga agaatttctt
     4201 ctccgtgagg agctgcgcga acgcgttgct tgccgcgagc agtgcggcaa gggcggcagc
     4261 ggctgcgatg cgaatcgtca tttgtccccc ctcctctttt gaacgctaca cccggtacgc
     4321 gaccgtagtc atgactttcg aagcaagtcg catcgcccgt ttgaccggct cggggagttc
     4381 agcggcaccg agctcgatcg cgctcgcgcg atgcctcgcc tcgtcggcct gcatggcttc
     4441 gaccaccgcg cgcgtgcggt cgtcctcggg cggcagctcc tcgagatggc cgccgagatg
     4501 ctcctcgacc tggcgctcgg tctccgccag gaacgcaaga ttccaccggt ctcccagcgc
     4561 gcccgccacg gcgccgatcg cgaaagcgcc cgcgtaccag agcgggttaa gcaggctcgg
     4621 ccggccgccg agctcgcgga tgcgctcgac gctccacgcg agatggtcct cttcctcgcg
     4681 cgcagcctgc tcgagcgcct ggcgcgcgac ggcgtcgcgc gcggtgagcg cctgaccctg
     4741 gtacagcgcc tgggcgcaca cctcgccgac gtggttcacg cgcatgagcg ccgcggcatg
     4801 ccggcggtct tcgtcggtga tttccttttc cgacactccc gccgccggcg aaggccgtgt
     4861 cgcgtgcgcc acgccggcga ccgcacgcaa cgcgcggtcg aattcgccga tcaaccggtc
     4921 gacggagatc atgcgtcggg gcagaaattc atccagccgg taattatgta cttatgctgg
     4981 ttcggcgaga ccacgccgac gtgcgtatgc gtgaagtcgg acggccagat gagcgtgcga
     5041 cccttcttcg cctcgaacgt gtgcttctgg tacacaaagt gcgtgccgcc gccgtccgtc
     5101 acgtcgttga ggtaagtcat ccacacgagc atgcgcgtcg cagtggcata gccgctgcgc
     5161 tcgaagtgcg gcatcttgaa gccgcccccg gggcgatagt gctggatggc aggcgactca
     5221 gtgacccgga acttgccaag ctgcttgagc atcggatgct gctccacata gagctgcagg
     5281 cagcgctgga gctcggcgta gtagcgctcg atcccgtact cactgaggag ctccgcaggc
     5341 acgaggtcca cgctgacgtc gaaagaatcc ttcttctccg gatcgaccac caggctgccg
     5401 ccctttccca tccggccgcg cttcaccagg cc
//
LOCUS       DASTPD010000201         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_99964, whole genome shotgun sequence.
ACCESSION   DASTPD010000201 DASTPD010000000
VERSION     DASTPD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99964"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFR57_06660"
     CDS             complement(<1..811)
                     /locus_tag="VFR57_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0259080.1"
                     /translation="MKARNADAVIVAGGGIGGLAAALALARKGFRSVVLEQASQFGEI
                     GAGIQIAPNAWHALDALGVGGLVKKEAVFIEHLLMFDGVSGEKVIDIPLDKRFAKRFG
                     NPYAVTHRADIHGSLLDGCKALPDLIELRTSTRVLGFEDDGKGVTVRTDKESIPGTAL
                     VGADGGRSVIRESIVGDKLPLITGHMCYRAVLNIEDVPKDLRFAAATLWAAHNAHIVH
                     YPLRGWRLFNLVATIIGKHTSGGHNELAQPDEVLPFFAHYCEKPLKLMRTPK"
     gene            complement(808..1611)
                     /locus_tag="VFR57_06665"
     CDS             complement(808..1611)
                     /locus_tag="VFR57_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259081.1"
                     /translation="MTKVFLGYDQKALDDQYEQRVWVPHADQIIKRYAEKSDAVRKRL
                     GEPRVQRYGPTPPETLDLYGSGKQAFVFVHGGAWKRQSSREQGFLAEPIVQAGATYIA
                     LNFALLPTVTMAEMVAQVCRALEWIRKNLSDDIALCGHSSGGHLAACALIKTNFVRRA
                     MVVSGIYDLLPVRLSARNDYVRVDDRSEHEYSPIRHVAKIKCPVTVGWAEKESAEFVR
                     QSKQFAAALRAAGKLSESLEGESLNHFEILETMADPRSPLGRAALNMLE"
     gene            complement(1608..2066)
                     /locus_tag="VFR57_06670"
     CDS             complement(1608..2066)
                     /locus_tag="VFR57_06670"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEU0259082.1"
                     /translation="MNVEQFLYHEARLLDTGQFEAWLDLFTEDATYWLPLERDQTDPL
                     ETSSIIHDDRTLLELRVRQARHPRAHARQPLARTVHQVGNVMVLSENGSDIMVASTLN
                     LVEFRNERQRNYGALVEHRLRRAGDSYRIAHKRVDLVNSEGELDGIAILF"
     gene            complement(2063..>2767)
                     /locus_tag="VFR57_06675"
     CDS             complement(2063..>2767)
                     /locus_tag="VFR57_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0259083.1"
                     /translation="HLDGAHPAWVHASSVAVARNAPEPGKPGEEHYYDIAVRQMRQNG
                     APDAVWEAIGMWTTPHGHGYMGDYHDDSRLVVGMGNPVFEQYRAKLSEEHGPQKADRI
                     LRVTLWNTIIYPNCSFMSQFRQLRIIHPIAVDRSVVYTYSFKMTKAPPQMFRDTIAFS
                     NVVNGTGSWVLTDDLEVYERIQRGFSSAAVDWAYIGRGYGRDLKEPNGSSRGATGTSE
                     IFVRAQFAAWLRYMSQ"
BASE COUNT          454 a          872 c          896 g          545 t
ORIGIN      
        1 ctttcggcgt gcgcatgagc ttgagcggct tttcgcagta gtgcgcgaag aaggggagca
       61 cctcgtcggg ctgcgcaagc tcgttgtgac cgccgctcgt gtgcttgccg atgatcgtgg
      121 cgacgaggtt gaaaagcctc cagccgcgca gcgggtagtg gacgatgtgc gcgttatgcg
      181 cggcccaaag cgtcgccgcg gcgaagcgca aatccttggg cacgtcctcg atgttgagca
      241 ccgcgcggta gcacatgtgc ccggtgatga gcggcagctt gtcgccgacg atgctttcac
      301 ggatcaccga tctcccgccg tccgcaccca ccagggcagt accgggaatc gactctttgt
      361 ccgttcttac ggtgactcct tttccatcat cctcaaatcc caggacgcgc gtgctggtcc
      421 gcagctcgat caagtccggc agcgccttac agccgtcgag gagcgagccg tggatgtcgg
      481 cgcggtgggt taccgcgtag gggttgccga agcgcttcgc gaagcgctta tcgagaggga
      541 tgtcgatcac cttctcgccg ctcaccccat cgaacatgag gaggtgctcg atgaagaccg
      601 cttccttctt caccaggccg cccacgccga gcgcgtcgag cgcgtgccag gcgttgggcg
      661 cgatctggat gccggccccg atctccccga actgggaggc ctgctccagc actaccgagc
      721 ggaagccttt gcgcgcgagg gcgagcgccg ccgcgagccc gccgatgccg ccgccggcga
      781 cgatgacggc gtctgcgttc cgcgctttca ttccagcatg ttaagcgctg cccggccgag
      841 cggggagcgg gggtcggcca tcgtttcgag gatctcgaag tggttgaggc tttcgccttc
      901 caaggattcg ctcagcttgc cggccgctct cagtgcggcg gcgaattgct tcgattgccg
      961 aacgaattcc gccgattcct tttccgccca gccgaccgtc accgggcact tgatcttggc
     1021 gacgtggcgg atcggcgagt attcgtgctc cgagcggtcg tccacgcgca cgtaatcgtt
     1081 gcgcgcgctc aggcgcacgg gcaggaggtc gtagatcccc gaaaccacca tcgctcttct
     1141 gacgaagttc gtcttaatca aggcgcacgc ggccagatgc ccgccggagg agtggccgca
     1201 gagcgcgatg tcatcgctca ggtttttcct tatccattca agcgcccggc agacctgcgc
     1261 caccatctcg gccatggtca cggtcggcag cagggcgaag ttgagcgcaa tatacgttgc
     1321 gccggcctgg acgatgggct ccgcgaggaa cccctgttcg cggctggact ggcgcttcca
     1381 cgcgccgccg tggacgaaga caaacgcttg tttacctgag ccatacagat ctagcgtttc
     1441 gggcggcgtc ggcccgtagc gctgtacgcg gggttctccc agccgcttcc ttacggcgtc
     1501 gctcttctcc gcgtaccgct tgatgatctg atcggcgtgc gggacccaga cgcgctgctc
     1561 gtactggtcg tcgagcgcct tctggtcgta gccgaggaag accttcgtca aaacaggatc
     1621 gcgatgccgt cgagctcgcc ttcggagttc acgagatcga cgcgcttgtg cgcgatgcga
     1681 tagctgtcgc ccgcgcggcg cagtcggtgc tcgaccagcg cgccgtagtt tctttgcctc
     1741 tcgtttctga actccacgag gttaagcgtc gaggctacca tgatgtcgga accgttctcc
     1801 gaaagcacca tcacgttacc cacctggtgc acggtgcgcg cgagcggctg gcgcgcatgt
     1861 gcccgcggat gccgcgcctg cctgacgcgc agctcgagca gcgtgcggtc gtcgtgaatg
     1921 atcgagcttg tctccaaagg gtccgtctgg tcgcgctcga gcggcagcca ataggttgca
     1981 tcttcggtga agagatcgag ccaggcctcg aactgacccg tgtccagcag ccttgcctcg
     2041 tgatagagga attgctctac gttcactggg acatgtaacg cagccacgcg gcgaactggg
     2101 cgcggacgaa gatttcgctg gtgccggtgg cgccacggga ggagccgttc ggctccttca
     2161 agtctctacc gtagccgcgt ccgatgtagg cccagtccac cgcggccgag gagaagcctc
     2221 tctgaatgcg ctcataaacc tcgaggtcgt cggtcagcac ccaggacccg gtgccgttca
     2281 ccacgttgga gaaggcgatc gtgtcgcgga acatctgcgg cggcgctttc gtcattttga
     2341 aggagtaggt gtagaccacg gaacgatcga ccgcgatggg atggatgatg cgcagctgcc
     2401 gaaactggct catgaacgag cagttgggat agatgatcgt gttccagagg gtgacgcgca
     2461 ggatccgatc ggctttttga gggccatgct cttcgctgag tttcgccctg tattgctcaa
     2521 aaacggggtt acccatgccg acgacgaggc ggctgtcgtc gtgatagtcg cccatgtagc
     2581 cgtggccgtg cggcgtagtc cacatgccga tcgcttccca cactgcatcg ggcgcgccgt
     2641 tctggcgcat ctggcgcacc gcgatgtcgt agtagtgctc ctcgcccggc ttgcccggct
     2701 ccggggcgtt gcgcgctacc gcgaccgagg aggcgtgcac ccaagccggg tgcgcgccgt
     2761 cgagatg
//
LOCUS       DASTPD010000202         7273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1326786, whole genome shotgun sequence.
ACCESSION   DASTPD010000202 DASTPD010000000
VERSION     DASTPD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326786"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFR57_06680"
     CDS             <1..1063
                     /locus_tag="VFR57_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU0259084.1"
                     /translation="QPFNLGSPKQIQEILFDRQKLPVKKKTPSGQPSTDEDSLAELAL
                     DHPLPRLILEHRALSKLKSTYTDKLPKSINERTGRVHTTYSQITAVTGRLSSNEPNLQ
                     NIPIRTPAGRRIRECFIAPPGAKIVSADYSQIELRIMAHLSGDPSLRRAFAQGEDIHR
                     ATAAEVFGVPLSQVDPDQRRVAKVINFGLIYGMSSFGVAQNLGIDRSTAQTYIERYFA
                     RYPGAKRYMDETRARARELGYVETVFGRRLWLPELKSGAPVRRQAAERAAINAPMQGT
                     AADLIKLAMIAVQNFLDKEKLKALLIMQVHDELVFEVPDNELETVRASVPELMQSVAK
                     LDVPLVVEVGVGNNWDKAH"
     gene            complement(1060..1734)
                     /locus_tag="VFR57_06685"
     CDS             complement(1060..1734)
                     /locus_tag="VFR57_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEU0259085.1"
                     /translation="MRSAILAAFVVTFAAPAAALLEGLTQREALAGLQTALERGARSA
                     VATLGQFDGFLGNPHVRIPLPDSLARTERLARRLGFGAEADELVVAMNRAAEAAMPEA
                     RKLLVDAVKKMSIEDAKAILTGGDTAATQYFRKTSHAALHDRFLPVVKAATEKVGVAQ
                     RYRELAQPAAAFGLVKAEDADLDEYVTLKTLDGLYFMLGEEEKKIRKDPVGTGSAILR
                     RVFGAL"
     gene            complement(1815..2699)
                     /gene="lipA"
                     /locus_tag="VFR57_06690"
                     /pseudo
     CDS             complement(1815..2699)
                     /gene="lipA"
                     /locus_tag="VFR57_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(2734..3339)
                     /gene="lipB"
                     /locus_tag="VFR57_06695"
     CDS             complement(2734..3339)
                     /gene="lipB"
                     /locus_tag="VFR57_06695"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU0259086.1"
                     /translation="MTVVDHIVVRRLGRADYFATWEAMRSFTRARDDSTPDEFWVLEH
                     PPVYTVGQGAPVPSIHSEVPVIKTDRGGDITYHGPGQVVIYTLVDLARRSIKVKRFVW
                     LLEQAVIDLLCGRAERETGAPGVYVDGAKIAALGIRVVRGRAYHGVALNVDMELSPFS
                     AIDPCGYPGLRVTQMKDLGFAETPATVASCLSQILIKKLDA"
     gene            complement(3336..3548)
                     /locus_tag="VFR57_06700"
     CDS             complement(3336..3548)
                     /locus_tag="VFR57_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEU0259087.1"
                     /translation="MGRKQPRFVQSVTGIVRKHAPDFDAATVEMRPSRQGKYLSVTCV
                     VRAISREQLDALYQELCDHPDVVMVL"
     gene            complement(3620..4492)
                     /locus_tag="VFR57_06705"
     CDS             complement(3620..4492)
                     /locus_tag="VFR57_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEU0259088.1"
                     /translation="MTVFLNGGLLPIEQATVSVLDRGFIFGDGVYELVPVYSRVPFRL
                     EEHLARLERSLGETRIRNPYSRAQWRAHIYALIDAQPFEDQGIYFQVTRGVAKRDHAF
                     PANVEPTVFMMSNPLVNPQPAQVEKGASAVSAQDYRWLRCDIKSISLIGNCLLRQISA
                     EAGGAETILFREGKLTEASASNVFIVKRGVIQSPPKSNLILPGITYDVVVELARANGL
                     PLEFKDIPEADVRAADELWVSSSSKEVLAIVELDGKRIGDGRPGPVFRRMFQLYQEFK
                     QKVMRAGKREAVSA"
     gene            complement(4489..5610)
                     /locus_tag="VFR57_06710"
     CDS             complement(4489..5610)
                     /locus_tag="VFR57_06710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0259089.1"
                     /translation="MRFFLALVASASFGVQAAAPQPPSIIGKSWVLGDLSSGQVLAAQ
                     KAEERFEPASLTKMMTAYVVFAALRDRKLALDQQLKVSERAWKAPGARMYLEPRRPVV
                     VEELIRGMVVQSANDACVALAEALAGSEEAFAQMMNREAERLGMKNTRFVNASGLPHA
                     QHYSTAQDLYNLAAALIRDFPHEYAQYYSQKEFRYNNIPQVNRNRLLWIDPSVDGVKT
                     GQTEAAGYCLVASSRRGGRRLLSVLLGSTSEATRAQESLKLLNWGYQFFDSVKLYAAG
                     ATVRSLEVWKGASRSVEVGVGADLFVTVPKGEADRLKAELVSQQPLVAPLGKGQRVGV
                     LRVTHDGRPFAEYPLVTLEPVAMAGFPRRTWDTLRLWLK"
     gene            complement(5661..6170)
                     /locus_tag="VFR57_06715"
                     /pseudo
     CDS             complement(5661..6170)
                     /locus_tag="VFR57_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(6167..>7273)
                     /locus_tag="VFR57_06720"
     CDS             complement(6167..>7273)
                     /locus_tag="VFR57_06720"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0259090.1"
                     /translation="FIDLSARLREPGYEYKATLESEMEAALAGGVTSLACPPDTDPPL
                     DEPGLVDMLRRRAKALSRARVYPVGALTVKLQGQALTEMGQLTDAGCVAFSQANAPLA
                     DTQVLWRALQYAATFGYPVWLRAEDAWLAKGGVAHDGEVATRLGLPGIPPFAETIALA
                     TLLELVRATRARVHVCRLSTAGGVELMRRAKQEGLPVSCDVAVHHVHLCDMDLGYFDS
                     HCRLEPPLRSQRDREALSRALAEGVVDCLCSDHTPVDDDGKHLPFAQAEPGATGLELL
                     LPLALKWGEERRLGLPATLARITSEPARILGVASGSLAAGAPADLAVFDPQAPLRIAS
                     ETLKSQGKNTPFLGYELAGRVRYTIVGGNVAYEA"
BASE COUNT         1285 a         2408 c         2370 g         1210 t
ORIGIN      
        1 ccaacccttc aatctcggtt cgcccaagca gatccaagag atcctgttcg accggcagaa
       61 gctcccggta aagaagaaga cgccctcggg ccagccctca accgacgagg actcgctcgc
      121 cgagctcgcg ctcgaccacc cgctgccgcg tcttatcctc gagcaccgcg cgctctccaa
      181 gctcaagtcc acgtacaccg acaagctacc gaagagcatc aacgagcgca ccggccgtgt
      241 gcacaccacg tactcgcaga tcaccgcggt caccggcagg ctctcgtcca acgagcccaa
      301 cctgcagaac atcccgatcc gcacgccggc cggccgccgc atccgcgaat gcttcatcgc
      361 gccgccgggc gcgaaaatcg tctcggcgga ctactcgcag atcgagttga ggatcatggc
      421 gcacctttcg ggcgatccga gcctgcggcg cgcgttcgct caaggggaag acatccaccg
      481 cgcgaccgcc gccgaagtct tcggtgtacc tttgtcccaa gtcgatcccg atcagcgccg
      541 cgtcgccaag gtgatcaact tcggcctgat ctacggcatg tcgtccttcg gcgtggcgca
      601 gaacctcggc atcgaccgct cgactgccca gacctacatc gagcgctact tcgcgcgcta
      661 ccccggtgcc aagcgctaca tggacgagac gcgcgcccgc gcgcgcgagc tcggctatgt
      721 cgagaccgtg ttcggccggc gcttgtggct ccctgagttg aagagcggcg cgccggtaag
      781 gcgtcaggcc gcagagcgcg cggcgatcaa tgcgccaatg cagggcacgg ccgcggacct
      841 catcaagctc gccatgatcg cggtccagaa cttcctcgac aaggaaaagc tcaaggccct
      901 tctgatcatg caggtgcacg acgagctcgt attcgaagtt cccgacaacg aactcgagac
      961 cgttagagcg agtgttccgg aactcatgca gtccgtcgcg aaactggacg tccctctcgt
     1021 cgtggaggta ggtgtaggaa acaactggga caaggcgcac taaagcgcgc cgaaaacccg
     1081 gcgcaggatc gcgctgccgg ttccgaccgg gtccttgcga atcttcttct cttcctcgcc
     1141 cagcatgaaa tagaggccgt cgagcgtctt gagcgttacg tattcgtcga ggtccgcgtc
     1201 ctcggccttg accaggccga acgcggccgc cggctgcgcg agctcgcggt agcgctgggc
     1261 gacgccgact ttctcggtcg cggccttcac gacgggcaga aagcggtcgt gaagcgcagc
     1321 gtggctcgtc ttccgaaagt attgcgtcgc ggcggtgtcg ccgccggtga ggatggcctt
     1381 ggcgtcctcg atgctcatct tcttcaccgc gtcgaccagg agcttgcgcg cctcgggcat
     1441 cgcggcctcg gcggcgcggt tcatggcgac gacgagctcg tcggcttcgg ctccgaagcc
     1501 gagccggcgg gcgagccttt cggtccgcgc gagcgagtcg ggcaacggga tccggacatg
     1561 cggattgccg aggaaaccgt cgaactggcc gagcgtcgct accgcgctgc gcgcgccgcg
     1621 ctcgagcgcc gtctgcaggc cggcaagcgc ctcgcgctgc gtcagtccct ccagaagggc
     1681 tgcggccgga gcggcaaacg tcaccacgaa tgctgcgaga atcgcgcttc tcatcaaggc
     1741 gaggattatc gtcgggctgt tcactcttgc cgcgggcata gttgtcccgc ggcgactacg
     1801 gccggtctca gctcctagag cccggcttgg tgcgcgtgct ggtccgcgtg gtacgacgag
     1861 cgcacaagcg cgccgaccgc ggcatgccgg aacccgaggc tcgccgcttc tctttcaaaa
     1921 agggaaaaaa cccgaggctc gacgtagcgc tccaccggca aatgatgcgg cgagggctga
     1981 aggtactgcc cgatcgtcag catgtcgacc tcgtgggcgc gcaggtcgcg catgaccgcg
     2041 aggatctcgt cgtccgtctc gccgagcccg accatcaggc ctgacttggt aggcactgcc
     2101 gggtgcagct tcttgaattc ctggagcaag cgcagcgagt gctcgtagtc gccgcccggg
     2161 cgcgccttac ggtagagccg cggcacggtt tccaggttgt ggttcatgac gtccggcggc
     2221 accgccttca ggatatcgag cgcgcgctcc agccgcccgc ggaagtcggg caccagcact
     2281 tcgaccgtcg tgtgcggggc agcatctcgc acggaacgaa tgcagtccac gaagtgctgt
     2341 gcgccgccgt cgcgcaggtc gtcgcggtcg acgctggtaa tgaccacgta tttcaggccg
     2401 agacgcgaga tcgtttcagc gagatgccgc ggctcttcag cgtcgggcgc gagtggccgg
     2461 ccatgcgcaa cgtcgcagaa cgggcagcgg cgcgtgcaga tgtcgcccag gatcatgaaa
     2521 gttgccgtgc ccttgccgaa gcactcggca atattcgggc acgacgcttc ttcgcacaca
     2581 gtgtgcagct tttgttcacg caggatgtgc ttgatctcgt agaagcgcga gttcgggctc
     2641 gcggcgcgca cgcggatcca cgccggcttc ggcagcggca cacgcggaac gatgcggatc
     2701 gtcttgcggg cgcctttttc ctttacgcca gcttcacgca tcgagctttt taatcagaat
     2761 ttgggacaag caagaagcca cggttgccgg cgtctcggca aaacccaggt ccttcatttg
     2821 cgtcacacgc agccccggat agccgcaggg atcgatggcc gagaacggcg agagttccat
     2881 gtcgacattg agcgccacgc cgtgataagc gcggccgcgc acgacgcgga tgccgagcgc
     2941 cgcgattttc gccccgtcga catacacgcc cggcgcgccg gtctcgcgct cggcgcgccc
     3001 gcagagcagg tcgatcacag cctgctcgag caaccacacg aagcgcttga ccttgatcga
     3061 gcgccgtgcg aggtccacaa gcgtgtagat cacgacctgc cccgggccgt ggtatgtgat
     3121 gtcaccgccg cggtcggtct tgataaccgg aacctcgctg tggattgacg gaactggcgc
     3181 gccctgccct accgtataga ccggcgggtg ctcgagcacc cagaactcgt cgggagtcga
     3241 gtcgtcgcgc gcccgggtga acgagcgcat ggcttcccag gtcgcgaaat aatccgcgcg
     3301 gccgagccgc cggacgacga tatggtcgac gaccgtcata ggaccatgac cacgtcggga
     3361 tggtcgcaca gctcctggta gagggcgtcg agttgctcgc gcgagatggc gcgcaccacg
     3421 caggtgacgc tcagatactt tccctgccgg ctcgggcgca tctcgactgt cgcggcgtcg
     3481 aagtcaggcg cgtgcttgcg cacgatgccg gtgaccgact gcacgaagcg cggctgcttg
     3541 cggcccatga ccttaatcgg aaaatcgcaa ggaaacgcta aaggcgagcc atccgctacg
     3601 accggcacgg ccgagcctct taagccgata cggcctcgcg cttgccggcg cgcatgacct
     3661 tctgcttgaa ctcctgatag agctggaaca tgcggcggaa cacggggccg gggcgcccat
     3721 cgccgatgcg cttgccgtcg agctccacga tagcgagcac ctccttggac gaggagctga
     3781 cccagagctc gtctgcggcg cgcacgtcgg cttccgggat gtccttgaat tccagcggca
     3841 ggccgttcgc gcgcgcaagt tcgactacca cgtcgtatgt gatgccgggg aggatcaggt
     3901 tcgacttcgg cggtgactgg atcacgcccc gcttcacgat gaaaacgttc gaagccgagg
     3961 cctcggtcag ctttccctcg cggaagagaa tcgtctccgc cccgcccgct tcggcggaaa
     4021 tctgccgcag caggcagttg ccgatgagcg agatcgactt gatgtcgcag cgaagccacc
     4081 ggtagtcctg cgcactcacc gccgaagcgc ccttctcgac ctgcgccggc tgcgggttca
     4141 ccagagggtt ggacatcatg aacaccgtcg gttccacgtt ggccggaaat gcatgatcgc
     4201 gcttcgcgac gccgcgggtg acctggaagt agatgccttg gtcctcgaag ggctgcgcgt
     4261 cgatcagcgc gtagatgtgc gcacgccatt gcgcgcgcga gtacgggttg cggatgcggg
     4321 tctcgcccag gctgcgctcc aggcgcgcga ggtgctcctc gagccggaaa ggcacgcgcg
     4381 agtacaccgg cacgagctcg tacacgccgt cgccgaagat gaagccgcgg tcaaggaccg
     4441 agaccgtcgc ctgctcgatc ggcaggagcc ccccgttcag aaaaaccgtc atttgagcca
     4501 taaccgcagt gtatcccagg tgcgccgggg gaatcccgcc atggccaccg gctcgagcgt
     4561 cacgagcgga tattcggcga agggcctgcc gtcgtgcgtg acgcgcagca cgccgacgcg
     4621 ctggcccttg ccgagcggcg cgacgagcgg ctgctgggaa acgagctctg ctttaagccg
     4681 gtccgcctcg cccttcggca cagtgacgaa gaggtcggcg ccgacaccga cctccacgct
     4741 gcgcgaggcg cctttccaga cctcgagcga gcgcaccgtg gcgccggcgg cgtaaagctt
     4801 aacgctgtcg aaaaactgat atccccaatt gaggagcttg agagactcct gcgcgcgcgt
     4861 cgcttccgag gttgagccga gcagcaccga cagcaagcgc cgtccgccgc ggcgcgacga
     4921 ggcgacgagg cagtaccccg cggcctcggt ctgtccagtc ttcacgccgt cgacgctcgg
     4981 gtcgatccac agcagccgat tgcggttcac ctgcgggatg ttgttgtagc gaaactcctt
     5041 ctgcgagtag tactgcgcat actcgtgggg gaaatcgcgg atcagcgcgg cggcgaggtt
     5101 gtacaggtct tgagccgtgc tgtagtgctg ggcgtggggt agcccagagg cattcacgaa
     5161 ccgggtgttc ttcatgccga gccgctcggc ctcacggttc atcatctggg cgaaagcctc
     5221 ctcgcttccg gcaagcgcct cggcgagggc gacgcaggcg tcgttggccg actgcaccac
     5281 catgccgcgg atcagctctt ccaccactac cggccggcgt ggctccaggt acatccgagc
     5341 tcccggggcc ttccaggcgc gctccgacac ttttaactgc tggtcgagcg cgagttttct
     5401 atcgcgcagt gcagcgaaca cgacgtacgc cgtcatcatc ttcgtgagcg acgcgggctc
     5461 gaagcgctcc tccgctttct gggcggcgag cacctgtccg ctcgagaggt cgccgagcac
     5521 ccaggacttg ccgatgatcg agggcggctg cggggcagcc gcttgaactc caaaagaagc
     5581 ggaggcgact aaagcgagaa aaaacctcat ccgcgtgccg aaaaagcggc ggattataag
     5641 cccgcggttc tgccgggcac gacggcctcc acgtcgacct gcgcttgtcc agcctgaata
     5701 aagccgagct tgtgcgcagc gacgtacgag acgtcgatga tgcgggtgcc gcggaacggg
     5761 ccgcggtcgt tgatgcgaac gactacgctc ctgccgttcg caaggttggt gacgcgcgcg
     5821 taactcggaa tgggcaacgt ggggtgcgcg gcagtcatgg cgtacatgtc atagacctcg
     5881 ccgcttgaag tcttctggcc gtggtaacgc ttgccgtacc agctcgccat tccgcgctgc
     5941 cgaaagggct gcagcgaagt catcggcaca taatcccggc caagcgcgtt gtacgggcgg
     6001 ttcgcgtact tatgcagtgg ctcgatgcgc ggcaccgcat cgaccacgcg ctcgaggtcc
     6061 gcaggcggcc tctccggcgg tccgtcgtcc aggtaatacc caccaggtct gcctgtggtc
     6121 ccgcacccgg cgaaggcgag gatcaacatc aaaagagcca gtcttgtcat gcttcgtacg
     6181 cgacgttgcc gccgacgatc gtatagcgca cccggcctgc aagctcgtag cccagaaatg
     6241 gcgtgttctt gccctggctt ttcagcgttt cggaggcaat gcgcagcggt gcttgcggat
     6301 cgaagaccgc caagtcggcg ggcgcgccgg cggcgaggct tcccgaagcg acgccgagaa
     6361 tgcgcgccgg ctccgaagta atgcgcgcaa gggttgccgg caatccgagc cggcgctcct
     6421 cgccccactt caacgccaac ggcaggagca gctcgagccc cgtcgcgccg ggctcggcct
     6481 gcgcgaaggg gagatgcttg ccgtcgtcgt cgaccggggt gtggtccgaa caaaggcagt
     6541 cgaccacgcc ttcggcaagc gcgcgcgaga gcgcttcgcg atcgcgctgg ctgcggagcg
     6601 gaggttcgag gcggcagtgc gaatcgaagt agccgaggtc catgtcgcaa aggtgcacat
     6661 gatgtactgc gacgtcgcag cttaccggga gaccctcctg cttcgcccgg cgcatgagct
     6721 cgacgccgcc cgcggtggag aggcggcaga cgtgcacccg ggcccgcgtg gctcgcacca
     6781 gctcaagcaa cgtcgcgagc gcaatggtct cggcgaaggg cgggataccc ggcaagccta
     6841 ggcgcgtcgc cacctcgcca tcgtgcgcta cgccgccctt ggccagccac gcgtcttcag
     6901 cgcgcagcca gaccggatag ccgaaagtcg cggcatactg cagggcgcgc cacagcacct
     6961 gtgtatctgc cagcggagcg tttgcctgcg agaaggcgac gcagccggcg tcggtgagtt
     7021 gacccatctc ggtgagcgcc tggccctgga gcttcacggt gagcgcgccc acgggataaa
     7081 cccgcgcccg cgacagcgcc ttggcgcgcc ggcggagcat gtcaaccaac cccggctcgt
     7141 cgagcggcgg gtcggtatcc ggcggacagg cgaggctggt gacgccgccg gcgagcgcgg
     7201 cttccatttc cgattccagc gtcgccttgt actcgtagcc gggctcgcgc agccgcgccg
     7261 agaggtcgat gaa
//
LOCUS       DASTPD010000203         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_715164, whole genome shotgun sequence.
ACCESSION   DASTPD010000203 DASTPD010000000
VERSION     DASTPD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715164"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VFR57_06725"
     CDS             complement(<1..1358)
                     /locus_tag="VFR57_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEU0259091.1"
                     /translation="MKRVDHAEASSVFLRSRTHAEFHDQRLWDLRQKRDREAHGIPEW
                     EELRNLASAIKEHTLSHLGDYLEQFERNATANGMHVHWARDAAEHNQIVFNLLESHRA
                     RVLVKSKSMLTDECEMRPFLETRGIEIVETDLGERIQQLDDEPPSHIVVPAVHKLRAD
                     VAEVFSKTIGTEKGKTDVPYLAESQRRHTRPYYLKAGAGMTGANFAVAETGSFVVCTN
                     EGNADLAANLPPLHIASIGIEKLIPRLADLGVFVRMLSRSALGSPITQITSHFRAPRP
                     GSEMHVVLVDNGRSERLGMEGFWPSLKCIRCGACMNTCPVYRRSGGLSYGATYSGPIG
                     VIVDPTFNLRKYSALPFASTLNGSCTRVCPVKIDIHDQIYRWRRVIAERNQLPMVKKE
                     AMRMAGKVLSSPKLYRMAVKAAQAGLDYLPRSMIYNPLNAWGKQREVPKTPKQSFRDW
                     YV"
     gene            complement(1355..2068)
                     /locus_tag="VFR57_06730"
     CDS             complement(1355..2068)
                     /locus_tag="VFR57_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU0259092.1"
                     /translation="MKVGLFIPCYVDAFFPEIGVATLELLERLGMKVSYPLDQTCCGQ
                     PMANSGCHEDAAPVERLFVKNFAKFDYVVTPSGSCTHHVRERAKLHRNTFELTEFLHD
                     VLKVREFPWAKFPHKVGLHIGCAGLRGLELASTSEIAGPAFSKPHALLSKVKGVQIVR
                     PTRADECCGFGGTFAVTEEPVSARMGYDKVNDHLAAGAEYIVTPDPSCMMHQKGCAER
                     LGLKLKFIHIAQVLNGARA"
     gene            complement(2092..>2617)
                     /locus_tag="VFR57_06735"
     CDS             complement(2092..>2617)
                     /locus_tag="VFR57_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259093.1"
                     /translation="AAKVKQAHAGVGSVSFTTCTLLTSQLGAKSIVAAYRGTGPALND
                     LVGGQVDYMCDQIVNLVEQIKGGSIKAYAIATAERSPALPDVPTTKEAGLPEYQVSAW
                     NALFAPKVMPKDVAAKLNATLVAALDDAATRKRLLDLGSVIPDKAGRSPEALQKLVES
                     EVKRWNPILTAYAQ"
BASE COUNT          414 a          827 c          864 g          512 t
ORIGIN      
        1 ttcacgtacc agtcgcggaa ggactgcttg ggcgtcttcg gcacttcgcg ctgctttccc
       61 caggcgttga gcgggttgta gatcatcgag cgcggcaggt agtcgagacc ggcttgcgcc
      121 gccttcaccg ccatgcggta gagcttgggg ctcgagagca ccttgccggc catgcgcatc
      181 gcttccttct tcaccatggg aagctgattg cgttcggcga tgaccctgcg ccagcgatag
      241 atctggtcgt ggatgtcgat ctttacgggg cacacacgag tgcagctgcc gttgagcgtc
      301 gaggcgaacg gcagggcgct gtatttgcgc aggttgaacg tgggatcaac gatcacgccg
      361 atcgggccgg aataggtcgc gccgtaggag agccccccgc tgcggcggta caccgggcag
      421 gtgttcatgc aggcgccgca gcggatgcac ttgagcgagg gccagaagcc ttccataccc
      481 aggcgctcgg agcggccgtt gtcgaccagc acgacgtgca tctcactccc ggggcgcggc
      541 gcgcgaaagt gcgacgtgat ctgcgtaatc ggcgagccga gggcgctgcg cgagagcatg
      601 cgcacgaaga cacccaggtc cgcgaggcgc gggatcagct tctcgatgcc gatcgacgcg
      661 atgtgcagcg ggggcaggtt ggccgcgaga tcggcgttgc cttcgttcgt gcaaaccaca
      721 aagctgccgg tctcggcgac ggcgaagttc gcgcccgtca tccccgcgcc ggccttgagg
      781 taatagggcc gcgtgtgccg gcgctggctc tctgcaagat acggaacgtc ggtcttgcct
      841 ttctccgttc cgatggtttt cgaaaaaacc tcggccacat ccgcgcgcag cttgtgcacc
      901 gcgggcacca cgatatggct cggcggctcg tcgtcgagct gctggatgcg ctcgccgagg
      961 tcggtctcga cgatctcgat gccgcgcgtc tcgaggaacg gccgcatctc gcactcgtcg
     1021 gtgagcatgg atttgctctt caccaggacc cgtgcgcggt ggctttccag cagattgaaa
     1081 acgatctggt tatgctctgc ggcgtcgcgc gcccagtgca cgtgcatgcc gttcgcggtc
     1141 gcgttgcgct cgaactgctc gagatagtcg ccgaggtgcg agagggtgtg ttccttgata
     1201 gccgaggcga ggtttcgcag ctcttcccat tcgggaatgc cgtgggcctc gcggtcgcgc
     1261 ttctgcctca ggtcccagag acgctggtca tgaaattctg catgggttct ggatctcaaa
     1321 aaaaccgaag aagcttcggc atggtctacc cgcttcatgc gcgcgcccca ttcaggacct
     1381 gcgcgatatg gatgaatttc agtttgagcc cgaggcgctc ggcgcagcct ttctggtgca
     1441 tcatgcaaga gggatccggc gtgacgatgt actccgcgcc ggcggcaagg tgatcgttca
     1501 ccttgtcgta gcccatgcgc gcggatacgg gctcctcggt cactgcaaac gtgccgccga
     1561 agccgcagca ctcgtcggca cgcgtgggcc tgacgatctg cactcccttc accttggaaa
     1621 gcagggcgtg cggcttggag aacgccggtc cggcgatctc gctggtgctc gcaagctcca
     1681 ggccccgcag gccggcgcag ccgatgtgca gcccgacctt gtgcgggaac ttcgcccacg
     1741 ggaattcgcg caccttgagc acgtcgtgca ggaattcggt gagctcgaag gtgttgcggt
     1801 gcagctttgc tctctcgcgc acgtgatgcg tacagctgcc cgacggcgtg acgacgtagt
     1861 cgaactttgc gaagttcttg acgaagaggc gctctaccgg cgccgcgtcc tcatggcagc
     1921 cgctgttcgc catcggctgg ccgcagcagg tctggtcgag cgggtacgac accttcatcc
     1981 ccagacgctc gagcagctcg agcgtcgcga cgccaatctc agggaagaag gcgtcgacgt
     2041 agcacgggat gaaaaggcct actttcatcc cgtgccgctg ccgttttttt gctactgcgc
     2101 gtacgccgtg aggatcgggt tccagcgctt cacttcgctc tcgaccagct tctgcagcgc
     2161 ctccggagaa cgtccggcct tgtcggggat cacgctgccc aggtcgagca ggcgcttgcg
     2221 cgtcgccgca tcgtcgagcg ccgccaccag cgtagcgttc agcttggctg ccacgtcctt
     2281 tggcatgact ttgggcgcga acagcgcgtt ccacgcgctc acctggtact ccggcaggcc
     2341 cgcttccttc gtcgtcggga catcgggcag cgcgggcgag cgctccgccg tcgcgatggc
     2401 gtaggccttg atgctgccgc ccttgatctg ctcgacgagg ttcacgattt ggtcgcacat
     2461 gtagtccacc tgcccgccga cgaggtcgtt gagcgccggg ccagtgccgc ggtatgcggc
     2521 gacgatgctc tttgcgccga gctgcgaggt gagcagcgtg caggtcgtga acgacaccga
     2581 gcccacgccg gcgtgcgcct gcttcacctt ggcggcg
//
LOCUS       DASTPD010000204         8330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_715456, whole genome shotgun sequence.
ACCESSION   DASTPD010000204 DASTPD010000000
VERSION     DASTPD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715456"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFR57_06740"
     CDS             complement(<1..841)
                     /locus_tag="VFR57_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0259094.1"
                     /translation="MLYTKLVLMALFWSGVFPAVNIVLSSMGLVTSVFVRFWFAAVIL
                     LVMLRLRAHRLPRPSPRETVLVIGLGILGISLYNVLFTAGLGLVEASRAALIVPTNPA
                     FTALFATLFLKERLGAVRAAGIALCVLGALWVLTKGNILTLRELDLGVGELLLVLCIF
                     MWSAYTLLGRVALSGLPALALTAYVMTAGSLMLAIPAWLEHDSLARATWQSWAALGYL
                     IVFGTVLPFLWFYEGVKALGAARAAQFINLVPPLAVAESVLILGEPMTPALYVGTALV
                     VTGL"
     gene            888..1271
                     /locus_tag="VFR57_06745"
     CDS             888..1271
                     /locus_tag="VFR57_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259095.1"
                     /translation="MKRVLGLALTAAVFSAFAQEQEIQRALIQRDQQSAEFAARVRGA
                     DTTRLEQLHTEQLRDAGRPLDPDPRVARELRPYERQRMADERVLVLPPPVVRTPPAPE
                     NQPRPITPPSRPRTLVDPIPAQTPY"
     gene            complement(1282..3915)
                     /gene="mdeB"
                     /locus_tag="VFR57_06750"
     CDS             complement(1282..3915)
                     /gene="mdeB"
                     /locus_tag="VFR57_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HEU0259096.1"
                     /translation="MTDLADIVSSAFWRVNPPDADPTETREWLEAFDALVETEGRERA
                     TFLLRKLLDHARARRVPLPPVLNTPYKNSIALAHQPQFPGNLELESRISAIVRWNALA
                     MVVRANRAHPELGGHIASYASAADLFEVGFNHFFRIEDLVYFQPHSAPGVYARAYLEG
                     RLSEAQLNHYRRETGRKGLSSYCHPYLMPEFWQFPTGSMGLGPINAIYQARFMRYLEN
                     RGLAQEASKGKNLRKVWCFIGDGEMDEPESLAGLSLAAREGLDNLVFVVNCNLQRLDG
                     PVRGNGSVVQELEGLFAGAGWNVIKLLWGSDWDPLFARDEDGILLKRLHETVDGEFQT
                     YAATDGRFNREHFFNKYPELRELVADMSDDDIDHLRRGGHDPVKIYAAYHAAAHHKGQ
                     PTVILAKTKKGYGMGHWGQGKMGTHQQKKLDDEALREFRDRFALPLSDDDVSQLRFFH
                     PGPASPEIKYLLERRQQLGGFLPARNTKAEKLSVPALAAPERNQSTTMVFVQLLSQLL
                     KDEKLGPRVVPIVADEARTFGMQTLFRQVGIYSALGQLYEPEDHDELLYYREAKDGQI
                     LEEGITEAGALSSWLAAATAYSAHGKPMLPFYIYYSVFGFQRVGDLIWASADSRARGF
                     LVGGTAGRTTLAGEGLQHQDGSSHLVAATIPNCRAYDPCFGYELAAIVEDGARRMLEA
                     QEDLFYYLTVANENYPHPAMPDGAREGILRGLYLFKEHSKPNVQLLGSGPILREVIAA
                     AELLEKDWSIASNVWSVTSFTELSRDGVRAERARRFGRKETSWIEECLSSSEGPVIAA
                     SDYVRALPELIRSFIKKPYTVLGTDGFGRSDTRAALRAFFEVDARHIAVAALAAIDER
                     RAREAIARYGIDPAAPPPWSR"
     gene            4148..4351
                     /locus_tag="VFR57_06755"
     CDS             4148..4351
                     /locus_tag="VFR57_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0259097.1"
                     /translation="MPTGTVKWFNDSKGFGFITPSEGGEDLFAHHTAIQMSGFKTLKE
                     GQKVEFEVKRGPKGLQAHNIKPL"
     gene            complement(4396..6246)
                     /gene="glsA"
                     /locus_tag="VFR57_06760"
     CDS             complement(4396..6246)
                     /gene="glsA"
                     /locus_tag="VFR57_06760"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEU0259098.1"
                     /translation="MCSIFAKNMNCRAALPASPILEYLESLHARFVELTDGQVADYIP
                     ELSHADPEWFGICIATRDGFVYEVGDSRQPFTIQSISKPFTYGLVLQERGEDRVLAKI
                     GVEPSGDAFNAISLRPQTGAPLNPLINAGAIAACGLVPGDTVEEKTARVLEAFSCYAG
                     RALDIDERVYDSESATGHRNRAIGWMLRNFEILADEPGPTLEAYFRQCSIRVTCRDLA
                     VMGATLANGGVNPVSGIRALSEHYVDNVLSVMATCGMYDFSGEWLYRTGLPAKSGVGG
                     GILAVQPGRIGIAVFSPRLDPHGNSVRGIAVCRALAADLGLHLFDAAQPAAPAVRLAT
                     TRRKVASKQRRPPVAAEHLRNAGKRLRLYHLQGPLAFATVEPLVRELMARAVETECFI
                     INLRMVQAMDPAATRLLAGTRREFARLGKVLVFSEAGAWWQRLVDAGVERDAIFADDD
                     FALEYGENLLLAQRFREPAWGAETALEKCALFAGFAAEELARLEPLLAKRRYQRGQTI
                     VAAGQSSDELFVLTRGSAMVSIPTDDGITRLDSFSSGMTFGDVAFIDRSPRSANVTAL
                     GPVECRVLTREAFARLDEEAPAIKIRLLQNLACGMTSMLRRLSRELAALK"
     gene            complement(6264..6338)
                     /locus_tag="VFR57_06765"
     tRNA            complement(6264..6338)
                     /locus_tag="VFR57_06765"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6304..6306),aa:Thr,seq:tgt)
     gene            complement(6377..6829)
                     /locus_tag="VFR57_06770"
     CDS             complement(6377..6829)
                     /locus_tag="VFR57_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEU0259099.1"
                     /translation="MAEIPTKPYLLRALFEWCVDNGYTPHLAVRVDSRTQVPAEYVKG
                     GEITLNISPNAVHKLQIGNDLVEFSARFGGVARQISLPIGNVYALYARETGHGMTFDT
                     EGPKHRVQARAESEAVPTNSKPPTALPAPSTDPKKPSGGGKPTLRRIK"
     gene            complement(6846..7445)
                     /locus_tag="VFR57_06775"
     CDS             complement(6846..7445)
                     /locus_tag="VFR57_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0259100.1"
                     /translation="MMQLYSGTTCPFSHRCRFVLYEKGMDFQVIDVDLYNKPEDIAVM
                     NPYNRLPVLVERDLILYESNIINEYIDERFPHPQLMPADPVMRARARLMLFNMEVELF
                     SQIEALESGKEKQVERARQHVTDRLIELAPVFTKTKHMLGDDFTMLDVAIAPLLWRLD
                     HFGIKLGKTAAPLMKYAERIFSRPAFIEALTPSEKVMRK"
     gene            complement(7480..8178)
                     /locus_tag="VFR57_06780"
                     /pseudo
     CDS             complement(7480..8178)
                     /locus_tag="VFR57_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199892.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
BASE COUNT         1494 a         2670 c         2751 g         1415 t
ORIGIN      
        1 acaaccccgt cactactagt gcggtgccga cgtagagggc aggtgtcatc ggctcgccca
       61 ggatcagcac cgactcggca accgcgagcg gcggcaccaa gttgatgaac tgcgcggcgc
      121 gcgctgcgcc gagcgccttc acgccctcgt agaaccagag gaacggaagc accgtgccga
      181 agacgatgag atagccgagc gccgcccagc tttgccaagt ggcgcgagcg agcgagtcgt
      241 gctcgagcca ggcagggatc gccagcatga gcgatccggc ggtcatcacg tatgcggtca
      301 acgcgagcgc cggcagcccg gacagcgcga cgcggcccag aagcgtgtag gcgctccaca
      361 tgaagatgca cagcacgaga agcagctctc cgacgccgag atccagctca cgcagggtaa
      421 ggatgtttcc tttggtcaag acccatagcg cgccgagcac gcacagcgcg atgccggcgg
      481 cgcgcactgc gcccaggcgc tccttgagga acagcgtcgc gaatagcgct gtgaaagccg
      541 ggttggtcgg cacgatcaac gccgcacggc tcgcctcgac gagcccgagg cccgcggtga
      601 agagaacgtt gtagaggctg atgccgagaa taccgagccc gatcacgagc actgtctccc
      661 gcggcgatgg ccgtggtagc cggtgcgcgc gcaggcgcag catgacgagc aggatcaccg
      721 cggcgaacca gaagcgcacg aacactgagg tcacgaggcc catgctcgac agcacgatgt
      781 tcaccgcagg gaacacgccg ctccagaaga gcgccatcag tacgagcttc gtgtagagca
      841 ttttttgcgc tgcatgctag catcggcgca tgcgcacgat tactcgagtg aagcgcgttc
      901 tgggcctcgc gctgacggca gcggttttca gcgctttcgc gcaggaacag gagatccagc
      961 gcgcactgat ccagcgcgac cagcaatccg cggagttcgc cgccagggtg cgcggcgccg
     1021 acaccacgcg cctggagcag cttcataccg agcagttgcg cgacgccggc cgcccgctcg
     1081 accccgatcc gcgcgtcgca cgcgagctca ggccctacga gcgccagcgc atggccgacg
     1141 agcgcgttct cgttctgccg ccgccggtgg tacgtacacc gcccgcgccc gaaaaccagc
     1201 cgcggccgat cacgccgccg agccggccgc gcacgctggt cgatccgata ccggcgcaaa
     1261 cgccttacta gccgcttcga ctcagcgcga ccagggaggc ggcgcagccg ggtcgatgcc
     1321 gtagcgggcg atcgcttcgc gggcacgccg ctcgtcgatt gccgcgagcg cggcgacggc
     1381 gatgtgccga gcgtcgacct cgaagaacgc gcgcagcgcg gcgcgcgtgt cgctccgccc
     1441 aaagccgtcc gtaccgagca ccgtatacgg tttcttgatg aaactcctta ttaactctgg
     1501 cagggcgcgc acgtaatcgc tcgccgcgat cacaggacct tcacttgaac ttagacattc
     1561 ctcgatccag ctcgtctcct tgcgcccgaa gcgccgcgcg cgctccgcgc gcacgccatc
     1621 gcggctgagc tcggtgaacg aggtgacgct ccacacattg gaggcgatgc tccagtcctt
     1681 ctcgagcagc tccgccgccg cgatgacctc gcgcaggatg gggccgcttc ccagcagttg
     1741 tacattgggt tttgaatgtt ctttgaacaa ataaagacca cgcaggatgc cttcgcgcgc
     1801 tccgtccggc atcgccgggt gcgggtagtt ttcgttcgcg accgtgaggt aatagaagag
     1861 gtcttcctgc gcctcgagca tgcggcgcgc gccgtcctcg acgatcgcgg cgagctcgta
     1921 accgaagcaa gggtcgtaag cgcggcagtt cgggatcgtg gccgcgacca ggtgcgatga
     1981 gccgtcctgg tgctgcagtc cttcgccggc gagcgtggtg cgcccggcgg tgccgccgac
     2041 gaggaagccg cgcgcgcgcg agtcggccga cgcccagatg aggtctccga cgcgctggaa
     2101 gccgaatacc gagtaataga tgtagaacgg cagcatcggc ttgccgtgtg cggagtacgc
     2161 cgtcgccgcc gcgagccacg atgacagcgc gcctgcctcg gtgatgccct cctccagtat
     2221 ctggccgtcc ttcgcctcgc ggtagtagag cagctcgtca tggtcctcgg gctcgtagag
     2281 ctggccgagc gccgagtaga tgccgacctg ccggaacagc gtctgcatgc cgaaggtccg
     2341 cgcctcgtcg gcgacgatcg gcaccacgcg cgggccgagc ttctcgtcct tgaggagctg
     2401 cgacagaagc tgcacgaaca ccatggtggt cgactggttg cgttccggag cggcgagcgc
     2461 cggcacggaa agcttttccg ctttcgtgtt tctcgcggga agaaaaccgc cgagctgttg
     2521 ccgtctctcc agcaggtact tgatctccgg gctggcaggc ccgggatgga aaaagcgaag
     2581 ctgcgaaacg tcgtcatcgg agagcggcag cgcgaagcgg tcgcggaatt cgcgcagcgc
     2641 ctcgtcgtcg agcttcttct gctggtgggt gcccatcttg ccctggcccc agtgccccat
     2701 gccgtagccc ttcttggtct tggcgaggat gaccgtgggc tgccccttgt ggtgcgcggc
     2761 ggcgtggtac gcggcgtaga tcttcaccgg gtcgtgtccg ccgcggcgca ggtggtcgat
     2821 gtcgtcgtcg ctcatgtcgg cgacgagctc gcgcagctcc gggtacttgt tgaaaaagtg
     2881 ctcgcggttg aagcggccgt cggtcgcggc gtaggtttgg aattcgccgt ccaccgtctc
     2941 gtgcaagcgc ttgagcagga tgccgtcctc atcgcgcgca aagagtggat cccagtccga
     3001 gccccagaga agcttgatga cgttccagcc cgcgccggcg aacaggccct cgagctcctg
     3061 tacgaccgag ccgttgccgc gcaccggtcc gtcgagccgc tgcaaattgc agttgacgac
     3121 gaagaccagg ttgtcgagcc cctcgcgcgc ggcgagcgaa agcccggcga gcgactccgg
     3181 ctcgtccatt tcgccatcgc cgatgaagca ccagacttta cgcagattct tccccttgga
     3241 cgcctcttgg gccagccccc tgttctcgag gtaccgcatg aaccgggcct ggtaaatggc
     3301 gttgatcggc ccgaggccca tcgagcctgt cggaaactgc cagaactccg gcatcaggta
     3361 cggatggcaa tacgacgaga gacccttgcg tcccgtctct cttctgtagt gatttagctg
     3421 agcttcgctg agacgaccct ccaagtaagc cctcgcgtac accccgggcg ccgaatgcgg
     3481 ctggaagtac accaggtcct cgatacggaa aaaatggttg aagccgacct cgaagagatc
     3541 cgccgccgag gcgtagctcg cgatgtggcc gccgagctcc gggtgcgccc ggttcgcgcg
     3601 cacgaccatg gcgagcgcgt tccagcgcac gatcgcggag atgcggcttt cgagctcgag
     3661 gtttcccggg aactgcggct gatgcgcgag cgcgatcgag ttcttgtatg gagtattgag
     3721 caccggcggc agcggcacgc gccgcgcgcg ggcgtgatcg agaagcttgc gcagcaggaa
     3781 ggtggcgcgc tcccggcctt cggtttcgac gagcgcgtcg aatgcctcga gccattcgcg
     3841 ggtttcggtg gggtcggcgt cgggcggatt gacgcgccag aacgcggagg aaacgatgtc
     3901 ggcgaggtcg gtcatccggc catggtaata gcccggtcgg cccggccgct atccccgtcc
     3961 ttcgtctgga gaaatagtgc taagatcccc ggcgcaggat gtgtttcaag cagcgaattt
     4021 gccgtcccct cctcgtcgcc tcaagcagcg cgtcggtagt ggcagtcatt tccaactctt
     4081 gattcttcgt gcccggcttc gtcgcgcccg gattcgtcgc ggcagctttt tgtagaggta
     4141 acacgtaatg ccaaccggta ccgtcaagtg gttcaatgat tccaaaggct tcggcttcat
     4201 caccccgagc gagggcggcg aggatctgtt cgcgcatcac accgcgatcc agatgtccgg
     4261 cttcaagacc ctgaaggaag gtcagaaggt cgagttcgag gtgaagcggg gacccaaggg
     4321 actccaggcg cacaacatta agccgctgta acaagcccac ggcacccgtc aagaaagccc
     4381 gcgcagcggg ctttcttatt tcagggcggc aagctcgcgg ctcaggcgcc gcagcatcga
     4441 ggtcatcccg cacgcgaggt tctgcaacag ccggatcttg atcgccggcg cttcctcatc
     4501 gagtcgggca aaagcctccc gcgtcagcac gcggcactcc accgggccga gcgcggtcac
     4561 gttagccgat cgcggcgagc gatcgatgaa ggcgacgtcg ccgaaggtca tcccggacga
     4621 gaaggaatcg aggcgggtaa tgccgtcgtc ggtcggaatg ctgaccatcg cggacccacg
     4681 ggtaaggacg aagagctcgt ccgagctttg accggccgcc acgatggtct gcccgcgctg
     4741 gtaacgacgc ttggcgagca agggctcgag gcgagcgagc tcctcggctg cgaagccggc
     4801 gaacagcgcg catttttcga gcgcggtttc ggctccccag gccggctccc ggaagcgctg
     4861 ggcgagcagc agattctccc cgtactcgag cgcgaaatcg tcgtcggcga agatcgcgtc
     4921 acgctcgacg ccggcatcga cgaggcgctg ccaccacgcg cccgcctctg agaaaaccag
     4981 caccttgccc agcctggcga attccctgcg cgtgcctgca aggaggcgcg tcgcggccgg
     5041 atccatcgcc tgcaccatcc gcagattgat gatgaagcat tcggtctcga ccgcgcgcgc
     5101 catcaactcg cgtaccaaag gctccaccgt cgcgaaggca agcgggccct gcaagtggta
     5161 cagccgcagc cgctttcccg cattgcgcag gtgctcggcc gcgaccggcg ggcgccgctg
     5221 cttcgaggcg actttccgac gagtagttgc gaggcgcacc gccggcgcgg ccggttgcgc
     5281 cgcgtcgaag agatggaggc caagatcggc tgcgagcgcg cggcacacgg cgatgccgcg
     5341 aacgctgttt ccgtgcggat cgaggcgcgg agaaaagacg gcgatcccga tgcgccccgg
     5401 ctgcaccgcc aaaatgccgc cgccgactcc gcttttcgcc ggcaagccag tccggtacag
     5461 ccactcaccg gagaagtcgt acatgccgca cgtcgccatc accgagagga cgttatccac
     5521 gtagtgctcg gagagcgctc gaatcccgga tacgggattg acgccgccgt tggcgagcgt
     5581 cgcgcccatg acggcaaggt cgcggcaggt cacccgaata gagcactgcc gaaagtacgc
     5641 ttcgagcgta ggacccggct catcggcgag gatctcgaaa ttgcgcagca tccagccgat
     5701 tgcccgattt cgatgccctg tcgcgctctc cgagtcgtag acgcgctcgt cgatgtccaa
     5761 cgcacggccc gcatagcagg aaaacgcttc gagcacgcga gcggttttct cctcgaccgt
     5821 gtctcccggg accagcccgc acgcagcgat cgcaccagca ttgatcagcg gattcaacgg
     5881 cgcaccagtt tggggccgca ggcttattgc gttgaacgca tcgcccgagg gctcgacgcc
     5941 gatcttggca aggaccctgt cctctccgcg ttcctgcagc acaagcccat acgtaaaggg
     6001 cttggagatc gactggatgg tgaagggctg gcgcgagtcg cccacctcgt agacgaaccc
     6061 gtcccgggtg gcgatgcaga tgccgaacca ctccggatcg gcgtgggaga gctcggggat
     6121 gtagtccgcg acctggccgt ctgtgagctc gacaaagcga gcgtgcagcg actccaggta
     6181 ctcgagaatc ggcgaggcag gcaacgcggc tctgcagttc atgttcttgg cgaagatgct
     6241 gcacaaaccg tgcctgtcga ggctggtgcc ggttgtccga atcgaacgga cgacctaccg
     6301 cttacaaggc ggttgctcta cccctgagct aaaccggccg aggcgcgatt atgcgccggt
     6361 tacggcgatg cggcggttac ttgatcctgc gtaatgtagg cttgccgccg ccagaaggct
     6421 tcttcggatc ggtcgagggc gcgggcagcg cggtgggcgg cttggagttg gtaggaacgg
     6481 cctcggactc ggctctcgct tgcacgcggt gcttggggcc ctcggtgtcg aaggtcatgc
     6541 cgtgcccggt ctcgcgcgcg tagagcgcat agacgttgcc gatcgggagg gagatctggc
     6601 gcgcgacgcc gccgaagcgc gccgagaact cgaccaggtc gttgccgatc tggagcttgt
     6661 gcacggcgtt ggggctgatg ttcagcgtga tctcgccgcc cttcacgtat tcggccggca
     6721 cctgggttct cgagtcgacg cgcaccgcga ggtgcggcgt gtagccgttg tccacgcacc
     6781 actcgaaaag ggcgcgcaga aggtacggct tggtcgggat ctcagccatt gatgaggcgt
     6841 gtatcttact tgcgcatcac cttctcggaa ggcgtgagcg cttcgatgaa agcggggcgc
     6901 gagaagatgc gctcggcgta cttcatgagc ggcgccgcgg tcttgccgag cttgatgccg
     6961 aagtggtcca ggcgccacag gagcggcgcg attgccacgt cgagcatggt gaagtcgtcg
     7021 cccagcatgt gcttggtctt ggtgaacacc ggcgcgagct cgatcaggcg gtcggtgacg
     7081 tgctggcgcg cgcgctcgac ctgcttctcc ttgcccgact ccagcgcctc gatctgcgaa
     7141 aagagctcca cctccatgtt gaagagcatc aggcgtgccc gcgcgcgcat caccgggtcg
     7201 gcgggcatca gctgcgggtg cgggaagcgc tcgtcgatgt actcgttgat gatgttggac
     7261 tcgtacagga tgaggtcgcg ctccaccagc acgggcaggc ggttgtacgg gttcatcacc
     7321 gcgatgtcct cgggcttgtt gtacaggtcg acgtcgatga cctggaaatc catgcccttc
     7381 tcgtacagca cgaagcggca gcggtggctg aaagggcagg tggtgcccga atagagttgc
     7441 atcatgacct tggttcctgc ggaaagagag cgcggctctc tacttcacgt ccttccagaa
     7501 ttgcttcttc atccaatagg tgagcggaaa gaacaccacg agcagcacga agagcacgac
     7561 gatgcctatc gccttgcgct cggcggctgc gggctcgcct acgtagacca ggaagttcac
     7621 gaggtcgcgc tacgtcgcgt cgtactcggc aggcgactgc gagcctttcg agacctcggt
     7681 gagccggtgc tccttgtcgt cccagccgcg ctcgccctgc agcgcccaca gcgcgtgcgg
     7741 cattgcgacg ttgggataca ccgcgttgtt ccatcccgtg acggtcttcg ggtcgcgata
     7801 gaacgtgcgc aagtaggtgt agagccagtc ggcgccgcgc gagcgcgcga cgacggagag
     7861 gtccggcggc gcgacgccga accagcgctt gccctccttc ggatcgagca cgaccctgat
     7921 cgggtcgccg accttctccg ccgcaaagag caggtgctcc ttgatctgcc cttcggacag
     7981 gccgaactcg gtgaaccctg tgtagcgcat gtactgcagg ccgtggcagc cgaggcagta
     8041 gttgacgtag gtgcgggccc cggattgcag gctcaccagg ttgcgcgagt cgtgcggcga
     8101 gcggtcgagg cggtagccgc cgctcgcggc aagcgccatc gccggcatga aaagcagggc
     8161 ggcgaacaag ctcttcatac ggtcacccgc tcggggaccg gccgcacgcg atcgcgcgcg
     8221 gtgtaccagg gcatgaggaa aaagaagagg aaatacacaa ccgtgcacag ccttgcaacc
     8281 caggtcgcga cgtccttcgt gtcgaggatg tcccacggat cgcggaactg
//
LOCUS       DASTPD010000205         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1327248, whole genome shotgun sequence.
ACCESSION   DASTPD010000205 DASTPD010000000
VERSION     DASTPD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327248"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..874
                     /locus_tag="VFR57_06785"
     CDS             77..874
                     /locus_tag="VFR57_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0259101.1"
                     /translation="MTAIPNHALRQLRAGKLAIGMGVNQARTVGIATMAKTAGFDWLF
                     IDSEHGSLDGDTVAQIATASLAAGVTPIVRVAGFEHWHASRMLDNGAQGIVFPQVDSS
                     QDAQRVANACRYPPIGKRSMGGALAQLGFVSMPVGEAARLVNEETLVVVMIESPQGVA
                     NCEEIAAVKGVDALLIGTNDLCFEMGIPGQFNDPRVAEAYRKVIAACRKHGKFPGMGG
                     MYTPELLERHIAMGVQLILSGSDFSLLMQAATARAALVRGFEKKIGV"
     gene            complement(908..1273)
                     /locus_tag="VFR57_06790"
     CDS             complement(908..1273)
                     /locus_tag="VFR57_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259102.1"
                     /translation="MNLPKLPIETKPFLWGAVAGAITLAIVGFTWGGWVTSTTVERLA
                     GARADAATVAALTPICVTQFQASSRAPASLAALKGIQSWEQADYVSKGGWATMPGDNA
                     APSREVAAACAAALIKHAS"
     gene            complement(1306..1653)
                     /locus_tag="VFR57_06795"
     CDS             complement(1306..1653)
                     /locus_tag="VFR57_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0259103.1"
                     /translation="MDRSRIDSARRRLRYAWILVSMLAALGAFAGASAQDKGARFDDA
                     VITQRVNGALDADPVLRQMHIAVDTRDGVVHLTGFVDSTAQIDRAAALARRIEGVSAV
                     RNAIRVINRPSRA"
     gene            complement(2344..2898)
                     /locus_tag="VFR57_06800"
     CDS             complement(2344..2898)
                     /locus_tag="VFR57_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU0259104.1"
                     /translation="MKWEMHENSLFAVLLRSPWWVSLAIAIAIAAALRIFLPLEFAIF
                     GGLPFVVIACVAGWRQFKRPGAKRIATTLERARGLSADAFRAALEEGYRRQGYSAKRG
                     DGAADLVITQRGIVTLVGCRRWKAARTGIEPLREFDAATSEQGAHKRLYVAVGEVSDA
                     ARAFAAKKAIKLVDGEELATLLPK"
     gene            complement(2964..3158)
                     /locus_tag="VFR57_06805"
     CDS             complement(2964..3158)
                     /locus_tag="VFR57_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEU0259105.1"
                     /translation="MKVNYDAKTDTLTVIFRDVPVAESDEEKPGVILDYDAAGNIVSI
                     EVLDASHRVEEPGRVTYTEG"
BASE COUNT          548 a         1118 c         1064 g          619 t
ORIGIN      
        1 aattccagcg ccgtgttcgc atccatccga acgtaagcga gctccttccc ttcgtcctcg
       61 aggacctgaa accgctatga ccgctatccc caatcacgca ctgcgccaac tccgcgccgg
      121 caagctcgcc atcggcatgg gcgtcaacca ggcgcgcacc gtcggcatcg cgaccatggc
      181 gaagaccgcc ggcttcgact ggctcttcat cgacagcgag cacggctcgc tcgacggcga
      241 caccgtggcg cagatcgcca ccgcttcgct cgccgccggc gtgacgccga tcgtgcgcgt
      301 cgcgggcttc gagcactggc acgcctcgcg catgctcgac aacggcgccc agggcatcgt
      361 gtttcctcag gtcgattcga gccaggacgc gcagcgcgtg gcaaacgcct gccgctatcc
      421 acccatcggc aagcgctcca tgggcggcgc gctcgcgcaa ctgggctttg tctcgatgcc
      481 ggtgggcgag gcggcgcggc tcgtgaacga agagacgctc gtcgtggtga tgatcgagtc
      541 gccgcagggc gtggcgaact gcgaagagat cgccgcggtg aaaggcgtgg acgcgctcct
      601 catcggcacc aacgacctgt gcttcgagat gggcatcccg ggccagttca acgacccgcg
      661 cgtcgccgag gcctacagga aggtaatcgc cgcctgccgc aagcacggca agttccccgg
      721 catgggcggc atgtatacgc ccgagctcct cgagcgccac atcgcgatgg gcgtgcagct
      781 catcctgagc ggctcggact tctcgctcct gatgcaggcg gcgacggccc gagcggccct
      841 cgtgcgaggc ttcgagaaaa aaatcggggt ctgaccccga ttttcccgtt ggggtctgac
      901 cccggaatta cgaagcgtgc ttgatcagtg ccgctgcgca ggcggcggcg acctcgcggc
      961 tcggagcggc gttgtcgccg ggcatcgtcg cccagccgcc tttactcacg tagtcggcct
     1021 gctcccagct ctggatcccc ttcagcgccg cgaggctggc aggcgccctg gagctcgcct
     1081 ggaactgtgt cacgcaaatc ggggtgagcg cggcgaccgt cgccgcatcc gcgcgggcgc
     1141 cggcaaggcg ctcgaccgtg gtgctggtca cccaaccgcc ccaggtgaat ccgacgatgg
     1201 cgagcgtaat cgcgcccgct accgcgcccc acaagaaagg tttggtttcg attggcagct
     1261 tgggaaggtt catggcgttt cctctatctg tttacggcag gccagtcatg cgcgcgacgg
     1321 ccggttgatt acgcggatcg cattgcgcac cgcgcttact ccctcgatgc ggcgcgcgag
     1381 cgccgcggcc cggtcgatct gggccgtcga gtcgacgaag ccggtgagat ggaccacccc
     1441 gtcgcgggtg tccacggcga tatgcatttg cctcaggacc ggatcggcgt cgagcgcgcc
     1501 gttcacgcgc tgcgtgatga ccgcgtcgtc gaagcgcgcg cctttgtctt gcgcgctcgc
     1561 gccggcgaac gcgccgagcg cggcgagcat cgagaccagg atccacgcgt agcgcaggcg
     1621 cctgcgggcg ctgtcgatcc tgctgcggtc catgccgctt ccttcgcgtg gcgttagcct
     1681 gcggatcccg ccagggcggg ttgctgcgta gcgggagaga tctgctgaga atcgccggcg
     1741 ggcggcggtg gacgacaggt gacttccggg acgtagacca ccatacgcgg tcggcatcat
     1801 ccgcgtcaag gaaaatcggg gtcaggtagt gcctcagttt gatttgtaaa tagagttaag
     1861 tgcggcaccg cacggacgct gcatatccca aactcctata gttgtggacc tcggttgccg
     1921 cacgtctctt ctcttcgggg gatggggcgc atgggcgata ggatgtagat ggtctcgcgg
     1981 caaccgacca tgatttctct cttccagcgt ccagacgtga tcgccgacgc cagcctgcat
     2041 cgcaggcgtg acgcgcaggg acttatggat gcgggggatg cgggcgaagt tgtgcccgta
     2101 accacgccct ggagcgcgcc gaggcatttg ccggggctgt gtcggtgctc tgactctttc
     2161 ttgtcgccct gattgcctcc gtacagcttg accagcatcg cgtagtccac gtcgatccca
     2221 aacgcgcctt ccacggcgct caggtagcct ttgtgaccgt ccgtagttag ttgcacttgg
     2281 ggtctgaccc cgattttggg gaccgatttt tctcagccga tcaccacggc tgtaaccgaa
     2341 tgtttacttc gggaggagcg tcgccagctc ttctccgtct accagcttga tcgctttctt
     2401 ggcagcgaac gcacgcgcgg catcgctaac ttcgcccacc gccacgtaca ggcgcttgtg
     2461 cgcgccctgc tcgctcgtcg cggcatcgaa ctcgcgcagc ggctcgatgc ccgtgcgcgc
     2521 ggccttccag cgcctgcagc ccaccagggt cacgattccg cgctgcgtga tcacgagatc
     2581 ggccgcaccg tcgccgcgct tcgccgagta gccctggcgc ctgtagcctt cttcgagagc
     2641 ggcgcggaac gcgtctgccg acagcccgcg cgcgcgctcg agtgtggtgg cgatgcgctt
     2701 tgcgccgggc cgcttgaact gccgccagcc cgccacgcac gcaatcacca cgaacggcag
     2761 cccgccgaaa atggcgaact cgagcggcag aaagatccgc agcgccgccg cgatcgcgat
     2821 cgcgatcgcg aggctcaccc accacggcga gcgcagcagc accgcgaaca gcgagttctc
     2881 gtgcatctcc cacttcatga gcgatgcatt ctagagtcgc tcccctgata agggcgagcg
     2941 aagcgagcgg gggtcttagg ccactatccc tcggtgtagg tgaccctgcc aggctcttcc
     3001 acccggtgcg acgcgtcgag gacctcaatg gagacgatgt tgccggcggc gtcgtagtcc
     3061 agaatgacgc cgggcttctc ttcgtcgctc tctgcgacgg gaacatcgcg gaagatcacg
     3121 gtcagcgtgt cagtcttcgc gtcgtagttc accttcattc atctctccaa tatttacgca
     3181 gcttgctggt tcggtacgcg gtcaccacaa tgtcgtcgcc tgggcagagc tccaccacca
     3241 ctcgcactag ctgcagttta ccgcttgccg cgtcggtgat acgtgactgc cttatctcgc
     3301 gatcggatcg aacttaggaa aagaacgttc agcagctagg gcggttgac
//
LOCUS       DASTPD010000206         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1124051, whole genome shotgun sequence.
ACCESSION   DASTPD010000206 DASTPD010000000
VERSION     DASTPD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124051"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /gene="pilM"
                     /locus_tag="VFR57_06810"
     CDS             complement(<1..528)
                     /gene="pilM"
                     /locus_tag="VFR57_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU0259106.1"
                     /translation="MKFDLFTAKAPPLFGLDLSSSAVKMVELVDGGKSGYRVERYAIE
                     PLPRDCVVDGNISNLEAVTDSVKRCHARMGTRTKHVAMAVPSGAVISKKIIVPAALRE
                     DELEAQVESEANQYIPFALEEVNLDFQVLGPSPNSPDEQEVLIAATRKEKVEDRVAVA
                     DSAGLKALVMDVESFA"
     gene            799..>2711
                     /locus_tag="VFR57_06815"
     CDS             799..>2711
                     /locus_tag="VFR57_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0259107.1"
                     /translation="MVLAYPQLPALEDLGSQPKIPLRIYTADGVLIGEFGEERRAVIS
                     ITDVPQQLKNAVIAAEDERFYEHPGIDYLGVLRAAWANLVAGGRRQGASTITMQVARN
                     FFLSSEKTLTRKLYEALLAFKIEHSFSKEQILELYVNQIYLGQRAYGFAAASQTYFGK
                     ALGELSLGEVAMLAGLPKAPSLYNPVANPQRAKQRQQYVLRRMNELGYIDATQYEDAL
                     KAPLRARGREMAEYSVHAQYAAEMVRQALAEHYPEEVYTRGFRVYTTLRKADQEAAYR
                     ALRQGVLDYDRRAGYRGPEAYVELSANPTDEDVEDALDGHPDNDDLLAAVVLAYDGKQ
                     VQAGLRSGERIVIAGAGLQFAQRALDAKAAQQRRIRKGAIIRLQREGKGWQIVQLPEV
                     EAAFISLDPQDGAIRALVGGFDFGRNKFNHVTQAWRQPGSSFKPFIYSAALEKGFTAA
                     TVIADEPVVLEAEQTGSQRWEPKNYDGKFEGPMRMRTALAKSKNMVSIRILDAIGPKY
                     AQDYVTRFGFEAERHPAYLTMALGAGSVTAWQMARAYSVFANGGFLIQPYFIHKIVDD
                     RGNPLVLAEPRRAGDEALRVLDPRNAFIMDHMMQDVTRYGTAARAARLGRADLAGKTG
                     TTNEFVDAWFAGY"
BASE COUNT          504 a          899 c          831 g          477 t
ORIGIN      
        1 ggcgaacgac tcgacgtcca ttacgagagc cttcaatccg gccgaatcgg ccacggccac
       61 gcggtcctcg accttctcct tgcgcgtggc agcgatcagg acttcctgtt catccgggct
      121 gttgggcgag gggccgagga cttgaaagtc aagattcact tcctccaagg cgaacgggat
      181 gtactggttg gcctcgcttt ctacctgcgc ctcgagttca tcctcccgca aggccgccgg
      241 aacgataatc ttcttgctga tcaccgcgcc gctcggcacg gccatcgcca catgcttggt
      301 gcgtgtgccc atgcgcgcgt ggcagcgctt gaccgagtcc gtgaccgctt cgaggttgct
      361 gatgttgccg tcgaccacgc agtcgcgcgg caaaggctcg atcgcgtagc gctcgacgcg
      421 atagccgctc ttgccaccgt cgaccagctc gaccatcttc accgctgaag agctgaggtc
      481 cagcccgaac agcggtggcg ccttcgccgt aaaaaggtcg aatttcatct tatccttccc
      541 cgatatttcc cttttcgcgt cgcatcttag gcgcgattcg cagaaatggc gtgaaatgct
      601 agcaacaagc cctacggctg tctacttaat tttccacagg ggtagggcgt atgatcgcgc
      661 cgtccccggc accggttgca acaacgcctg ctccgaactg ctctaagtcc gaagaacatg
      721 tggatccggt acgcagccat acccctagcc atcgtggccg gtctagcgct cgcaacggtg
      781 ctggcggtag ggctggtgat ggtgctcgcc tacccccaac tgcccgctct tgaagacctg
      841 ggttcgcaac cgaaaatccc gctgcgcatc tataccgccg acggcgtgct gatcggcgag
      901 ttcggcgaag agcgccgcgc ggtgatctcc atcactgacg ttccacagca gctcaagaac
      961 gcggtcatcg ccgccgagga cgagcgtttc tacgagcacc cgggcatcga ctacctgggc
     1021 gtcctgcgtg cggcctgggc caacctggtc gccggcggcc ggcgccaggg agcgagcacg
     1081 atcacgatgc aggtagcccg caacttcttc ctgtcctccg agaaaaccct gacgcgcaag
     1141 ctctacgaag ccctgctcgc cttcaagatc gagcacagct tctccaagga gcagatcctc
     1201 gagttgtacg tcaatcagat ctacctgggc cagcgcgcct acggcttcgc tgctgcttcg
     1261 caaacgtatt tcggcaaggc gctgggtgag ctttcgctag gcgaggtagc gatgcttgcc
     1321 gggctgccca aggcgccctc gctttacaac ccggtcgcaa acccgcaacg cgccaagcaa
     1381 cgccaacagt acgtgctgcg gcgcatgaac gagctcggct acatcgatgc aacacaatac
     1441 gaggacgcgc taaaggcgcc gctgcgcgcg cgcggccgtg agatggcgga gtactcggtg
     1501 catgcgcaat acgccgctga gatggtgcgg caggcgctcg cggagcacta cccggaagag
     1561 gtctacacgc gcggctttcg cgtctataca accctgcgca aagccgacca ggaagccgcc
     1621 tatcgcgccc tgcgtcaggg ggtcctcgac tatgaccggc gggcgggtta tcgcggaccg
     1681 gaagcctatg tcgagctctc ggcgaacccg accgacgagg atgtggaaga cgcgctcgac
     1741 ggccacccgg acaacgatga cctgcttgcc gcggtcgtgc tcgcatacga cggcaagcag
     1801 gtgcaggccg gcttgcgcag cggcgagcgc atcgtcatcg ccggagctgg gctgcagttc
     1861 gcccagcgcg cgctcgacgc gaaggcagcg cagcagcggc gcatccgcaa aggcgccatc
     1921 atccgcttgc agcgcgaagg caagggatgg cagatcgtgc agctgcccga ggtggaagcg
     1981 gcgttcattt cgctcgatcc acaggatggc gcgatcaggg ccttggtcgg cggctttgat
     2041 ttcggccgca acaagttcaa ccatgtcacc caggcgtggc gccaacccgg ctcctcgttc
     2101 aagcccttca tctattccgc ggccctggag aagggtttca ccgccgcaac cgtcatcgcc
     2161 gatgagcctg tcgttctgga agccgagcag acgggcagcc agcggtggga accgaagaac
     2221 tacgacggca agtttgaggg cccgatgcgc atgcgaaccg cgctcgccaa gtccaagaac
     2281 atggtgtcga tccgcatcct cgacgcgatc gggcccaagt acgcacagga ctacgtgact
     2341 cgcttcggtt tcgaggccga gcgccacccg gcgtatctca ccatggcgct cggcgccggc
     2401 tcggtaaccg cgtggcagat ggcgcgcgcc tattcggtgt tcgcgaacgg cggcttcctt
     2461 atccagccgt acttcattca caaaatcgtg gacgatcgcg gtaacccgct cgtactggcc
     2521 gagccgcggc gcgcgggcga cgaggcgttg cgtgtcctcg acccgcgcaa cgccttcatc
     2581 atggaccaca tgatgcagga cgtgacccgc tacggcaccg cggcgcgcgc ggcgcggctc
     2641 ggccgcgcag accttgccgg caagacgggc acgacaaacg agttcgtcga cgcctggttc
     2701 gccggctatc a
//
LOCUS       DASTPD010000207         7132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_716860, whole genome shotgun sequence.
ACCESSION   DASTPD010000207 DASTPD010000000
VERSION     DASTPD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716860"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFR57_06820"
     CDS             <1..195
                     /locus_tag="VFR57_06820"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HEU0259108.1"
                     /translation="EKKLEAKLAALARVRGRTKSEVVREAILRMIEDTEDLEMAEKAL
                     RKTRSPKSLRRLRKELGLDR"
     gene            179..451
                     /locus_tag="VFR57_06825"
     CDS             179..451
                     /locus_tag="VFR57_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0259109.1"
                     /translation="MAWTVEVAEPAERQLRKLDPQVRRRILDWLDDRIEGCKNPRHFG
                     EALKGGRAGLWRYRVGDYRIICHIRDEVLVVLALAVGHRREIYRDR"
     gene            608..1915
                     /gene="rmuC"
                     /locus_tag="VFR57_06830"
     CDS             608..1915
                     /gene="rmuC"
                     /locus_tag="VFR57_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU0259110.1"
                     /translation="MLREARTRLEQVDKERVAALDELRVESSRRASFEALSQRIPDLE
                     QSLQYREQTILRHQQTILEGAREKEALAATIEAERRGFEEKQRLLEEAKAALSDAFGA
                     LSANALKSNSEQFVKLAHASVAPVRDALEKFEAKVQALEVAREGAYQGLVQQVTQLLD
                     TGKELRSETANLVQALRAPVTRGQWGEFQLRRVIEMAGMVNHCHFVEQKKVDTEDGQL
                     RPDVVVKLPAGKTIVIDAKAPVSSYMDAVVIEDETERRAKLANFAKLVREHIAALGRK
                     AYWDQFEASPELVVMFMPGDHYYSAALEQDPSLIEFGVEQKVLVATPVNLIGLLRAIA
                     FGWRQEAIATNATEISQLGAELYKRISDLGGHWVDVGKSLNKAVEAFNSTVGTLETRV
                     LVSARRFKDLGAAPASVDIEVIEPVVKAARSLKVLDIGTAKDS"
     gene            2012..4270
                     /locus_tag="VFR57_06835"
     CDS             2012..4270
                     /locus_tag="VFR57_06835"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0259111.1"
                     /translation="MPLSLRFVVPLALALAAIAYAIVPLVDRLTFQWFVRDLDMRGAL
                     IAQTAQDPLVELVKSGRGARERVLRYFDRISEGQRVFALGFCEPTGKMAYATRVFPEA
                     VRCSPARNERREPRNLVLELNDGALHVAAHPIVSDGQLLGELMIVHDMSFAQRRSADT
                     RKYVLWLFAGIAAVVALITVVIAEISWRGWVAGIKAVLSGDYLLRSPTAATKLPVPEL
                     RPFARDLQALVNDLENERRARDDSQTTWGPDALRRILRNDLKGDEILIVSNREPYLHV
                     RRKDNVIEIQRPASGLVTALEPVMRACSGTWIAHGAGSADRDTVDKHDHVMVPPEKPS
                     YRIRRVWLSKEEEQGYYFGFANEGLWPLCHIAHTRPTFRAPDWEHYQTVNRRFADVVA
                     DEARTDDPIVLVQDYHFALLPRMVRERLPRATIITFWHIPWPNPEAFGICPWREEVLE
                     GMLGSSILGFHTQFHCNNFFDTVDRFLEARVDRETFTISYGGDPTEVRRYPISIEWPP
                     SALAIQPPVAECRRRVRERLGLNPGVRLGIGVDRLDYTKGILERFAAIERLLEVEPRW
                     IGQFSFIQAAAPSRTSIDEYQNLDARVRQMAARINERFSRPGAPPIVLKIEHHDAAQV
                     YELYRASDLCYVSSLHDGMNLVAKEYVAARDDEQGVLILSQFTGAARDLAEALIVNPY
                     DTEQSAAAMQMALTMSPEEQRARMRSMRNLVQEFNVYRWAGRMLLDAARMRHRSRVMA
                     RARAARRRAAAF"
     gene            4274..5035
                     /gene="otsB"
                     /locus_tag="VFR57_06840"
     CDS             4274..5035
                     /gene="otsB"
                     /locus_tag="VFR57_06840"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU0259112.1"
                     /translation="MPPFERNWAFFLDLDGTLLEIEETPDAVDADYSELALVGHLLRA
                     ADGAVAMISGRSLQRLDEILAPLVVPAAGQHGAERRDARGVRHRHRFPANVLRPVAVG
                     IRRFASTHKGLVFEDKGASVALHYRLAPQLASVAHAKVREAAEPLGDQVEVQDGKMVV
                     ELKPAGCDKGKAIAEFMREPPFLGRVPVFLGDDVTDEYGFRVVNAMGGYSIKIGEGES
                     AARWRLQNPAAARAWLAQWIDFQNRGQDPFSKKGS"
     gene            complement(5087..6181)
                     /locus_tag="VFR57_06845"
     CDS             complement(5087..6181)
                     /locus_tag="VFR57_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259113.1"
                     /translation="MLDMLRQNIRRLLGALRRIEARGHSHGRALLLLPEVIVRRGYSA
                     FARHLDDFDLIHKINHRAFEAFQRNHAPVAGGHFYLIVMPGTLHFLVPCLSLLPVHMR
                     VILIANGAAEWECQYLARAFPRYPIFKLFTFPGSSLSHGTVLTLCLAWNERPFGVLDH
                     DCYVFDPSIFDDLEFGERDCLVAVFGNTAARLGIPYPETFFLYFNSTLLRPIMRRYRV
                     DARAYRKLPQTAREPLATIGIRDGVYLKEYHDYFDTLHVLLLLALTEGLTVRFLQSVD
                     ADSIAHLGSTSSGVAHTKDLMHSYVQLRFMELLNDVRVRHLYRRRTHPFRASSEVRSL
                     IPRAPEAVAKMNAFDALMLRLESRVRSAGV"
     gene            6420..>7132
                     /locus_tag="VFR57_06850"
     CDS             6420..>7132
                     /locus_tag="VFR57_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0259114.1"
                     /translation="MRLGVDVGGTKIEVVVLDATGAERMRRRTHYPSRDYQSALDCLV
                     ESIRAAQDECEQCSIGVGLPGFIDRETWFLENAFNTPYNKQPLKRDLEKALGKEVRFA
                     NDANCFALSEAVDGAARGARVVFGAILGTGAGGGLVVNGQVIEGRHGTAGEWGHTPLP
                     WMRQDEYPGPRCTCGRIGCIEQFVSGPAKNRERDTMGEEAAMTRFVDRLARAFAMMIN
                     IVDPDVIVVGGGMSKHDEI"
BASE COUNT         1325 a         2237 c         2353 g         1217 t
ORIGIN      
        1 gagaagaaac tcgaagccaa gctcgccgca ctggcgcgcg tgcgcgggcg caccaaaagc
       61 gaggtggtgc gcgaggcgat cctgcgcatg atcgaggata cggaagacct ggagatggcg
      121 gaaaaagcct tgcgcaagac ccgctcgccg aaatccctgc gccggctcag gaaagagctt
      181 ggcctggacc gttgaggtcg ccgaaccggc agaacgtcag ctccgcaaac tcgatcctca
      241 agtccggcgc cgcattctcg actggctcga cgaccgtatc gagggatgca agaacccccg
      301 gcatttcggt gaagcgctga aaggcggacg tgccggtttg tggcgctatc gagttgggga
      361 ctatcgcatc atctgccaca ttcgcgatga agtgcttgtc gtgctggctc ttgctgtagg
      421 ccatcgccgg gagatctacc gggatagata gcgcaagaac ttgcccaatg acctcccgtt
      481 gacgcagctg tattaatata cagcaccaac aataatggaa atcgcggcat tcctgcttct
      541 cgggctggcc ctcggaacgg cggccggttg gtttttccgc tcctcacgcg catcccagtc
      601 ggaggaaatg ctacgagaag cgcgcacgcg ccttgagcag gtcgacaagg aacgggttgc
      661 ggccctggac gagctgcgcg tcgaatcgag ccgccgagcg tctttcgagg ccctgtcgca
      721 gcgcataccg gacctggaac agtcgctcca gtaccgcgag cagaccatcc tgcgccatca
      781 gcagaccata ttggagggcg cgcgagagaa ggaggcgctc gcggcgacca tcgaggcgga
      841 acggaggggg ttcgaagaaa agcagcggct cctcgaagag gcgaaggcgg cgctttcgga
      901 tgcgttcggc gcgctttccg cgaacgcgct caagagcaac tccgagcagt tcgtgaagct
      961 ggcgcacgcg tcggttgcgc cggtgcgcga cgcgctggag aaattcgaag ccaaagtgca
     1021 ggcgctggag gtggcgcgcg agggcgcgta tcagggcctg gtgcagcagg tgacccagct
     1081 tctggatacc ggcaaggagc tgcgctccga gaccgcgaac ctggtgcagg cgttgcgtgc
     1141 tcccgtcacg cgcggccagt ggggcgaatt ccagctgcgc cgcgtgatcg agatggcggg
     1201 catggtgaac cattgccact tcgtcgagca gaagaaggtc gataccgagg acgggcagct
     1261 gcgccccgac gtggtggtga agctgccggc gggcaagact atcgtgatcg acgcgaaggc
     1321 gccggtgtcg agctacatgg acgcggtggt gatcgaggac gaaaccgagc gccgcgcgaa
     1381 gctcgccaat ttcgcgaagc tcgtgcgcga gcacattgcc gcgctgggcc gcaaagccta
     1441 ctgggaccag ttcgaggcct cgccggagct cgtggttatg ttcatgcccg gcgaccacta
     1501 ctacagcgcg gcgctcgagc aggatccgtc gctcatcgag ttcggcgtcg agcagaaagt
     1561 gctcgtcgcc acgccggtca atctcattgg gctcttgcgc gccatcgcgt ttggctggcg
     1621 ccaggaggcg attgccacca acgccacgga gatcagccaa ctcggcgccg agctctacaa
     1681 gcgcatctcc gatcttggcg gccactgggt cgatgtcggc aagagcctga acaaggcggt
     1741 cgaagccttc aacagcacgg taggcacgct cgaaacccgc gtgcttgtca gcgcccggcg
     1801 cttcaaggac ttgggcgcgg cgccagcctc ggtcgacatc gaggtgatcg agccggtggt
     1861 caaggcggcg cgttcgttaa aagtgctgga catcggcacg gcgaaggatt cgtaggcgct
     1921 cggctgtagc caatccgcga cagacaccgg ggtagccgcg gcttacaatc tattgggggc
     1981 gaaggggatt aaccggccga ttggagggct attgccgctt tcgctgcgct tcgtagtacc
     2041 gcttgctttg gcgctcgcgg cgatcgcata cgcgatcgtg ccgctggtgg accggttaac
     2101 ctttcagtgg ttcgtgcgcg acctcgacat gcgcggcgcg ctcatcgccc agaccgcgca
     2161 ggacccgctc gtcgagctcg tgaagagcgg ccgcggcgcg cgcgagcgcg tgctgcgtta
     2221 cttcgaccgc atctccgaag gccagcgtgt gttcgcgctc ggattttgcg agcccaccgg
     2281 caagatggcc tacgcgacgc gcgtcttccc cgaggcggtg cgctgttcgc cggcgcgcaa
     2341 cgagcgccgc gagcctcgca acctggtgct cgaactcaat gacggcgcgc tgcacgtcgc
     2401 cgcgcacccc atcgtctcgg acgggcagtt gctcggcgag ctcatgatcg tgcacgacat
     2461 gagcttcgcg cagcgccgca gcgcggacac gcgcaagtac gtgctctggc tttttgccgg
     2521 cattgcggcc gtcgtcgcgc tcatcacggt ggtgatcgcc gaaatcagct ggcgcggttg
     2581 ggtggcgggc atcaaggccg tgctctctgg cgattacctg ctgcgctcgc ccaccgcggc
     2641 gacgaagctg ccagtgcccg agctaaggcc ctttgcgcgg gatttgcagg cgctggtgaa
     2701 cgacctggag aacgagcggc gcgcccgcga cgatagccag acgacctggg gacctgatgc
     2761 cttgcgccgc atcctgcgta acgacctgaa gggcgacgag atcctcatcg tctcgaaccg
     2821 ggagccgtat ctgcacgtgc gccggaaaga caatgtgatc gagatccagc gtccggcgag
     2881 cggactcgtc accgcgctgg agccggtgat gcgcgcctgc tccgggacct ggatcgcgca
     2941 cggcgcgggg tccgccgacc gcgatacggt cgacaagcac gatcacgtga tggtgccgcc
     3001 ggagaagccg tcgtaccgca tccggcgcgt gtggctctcc aaggaggagg agcagggcta
     3061 ctacttcggc ttcgccaacg aggggctttg gccgctttgc cacatcgcgc atacgcggcc
     3121 gacgtttcgc gcgcccgact gggagcacta ccagacggtg aaccgccgct tcgccgatgt
     3181 ggtggccgac gaggcccgca cggatgatcc gatcgtgctc gtacaggact atcatttcgc
     3241 gctcttgccg cgcatggtgc gcgagcgact cccgcgcgcc accatcatca cgttctggca
     3301 tatcccctgg ccgaacccgg aagcgttcgg catctgcccg tggcgcgagg aagtgctgga
     3361 aggcatgctc ggctcctcga tcctcggctt ccacacgcag ttccactgca acaatttctt
     3421 cgacacggtc gaccgcttcc tcgaagcgcg cgtcgaccgc gagacgttca ccatttctta
     3481 cggcggtgac ccgaccgagg tacgccgtta cccgatctcg atcgaatggc cgccctcggc
     3541 gcttgccatc cagccgccgg tcgccgaatg ccgccggcgc gtgcgcgaac gcctcggcct
     3601 gaacccgggc gtgcgcctgg gcatcggcgt ggatcgcctc gactacacca agggcattct
     3661 cgagcgcttc gccgcgatcg agcggctgct cgaggtcgag ccgcgctgga tcggccagtt
     3721 ctcgttcatc caggcggctg caccctcgcg cacctcgatc gacgaatacc agaacctcga
     3781 cgcacgcgtg cgccagatgg cggcgcgcat caacgagcgc ttcagccggc cgggcgcgcc
     3841 cccgatcgtg ctcaagatcg agcaccacga cgcggcgcag gtgtacgagc tctatcgcgc
     3901 ctcggacctc tgttacgtct ccagcctgca cgacggcatg aacctggtgg cgaaggagta
     3961 cgttgcggcc cgcgatgacg agcagggcgt gctcattctt tcgcagttca ccggcgccgc
     4021 gcgcgatctc gccgaggcgc tcatcgtcaa tccgtacgac accgagcaga gcgccgccgc
     4081 gatgcaaatg gcgctcacca tgtctcccga agagcagcgc gcgcgcatgc gcagcatgcg
     4141 caacctcgtt caggagttca acgtgtaccg ctgggccggt cgcatgctgc tcgacgccgc
     4201 gcgcatgcgc caccgcagcc gcgtcatggc gcgcgcgcgg gcagcgcggc gccgcgccgc
     4261 cgctttctga ggaatgccgc cattcgagcg taactgggcc tttttcctcg atctcgacgg
     4321 cacgctgctc gagatcgagg aaacgccgga cgcagtcgat gccgactaca gcgagctcgc
     4381 gctcgtcggg catctcctgc gtgcggcgga tggcgcggtc gcgatgatca gcggccgctc
     4441 gctccagcgc ctcgacgaga ttcttgcgcc gctcgtcgtg ccggcggccg gccagcacgg
     4501 cgccgagcgc cgcgatgccc ggggggtgcg ccatcgccac cgctttccgg ccaacgtgct
     4561 ccgacccgtc gccgtcggca tccggcggtt cgcctccacg cacaaaggcc tggtgttcga
     4621 agacaagggc gcgagcgtcg cgctgcacta ccggctcgcg ccgcagcttg cctcggtggc
     4681 gcacgccaag gtacgcgagg ccgccgagcc gctcggcgac caggtcgagg tgcaggacgg
     4741 caagatggtg gtggagttga agcccgccgg gtgcgacaag ggcaaggcga tcgccgaatt
     4801 catgcgcgaa ccgcctttcc tcggccgcgt gccggtgttc ctgggcgacg acgtcaccga
     4861 cgagtacggc tttcgcgtcg tgaacgccat gggcggctac agcatcaaga tcggcgaagg
     4921 cgagagcgcc gcgcgctggc gcctccaaaa cccggccgcg gcgcgcgcat ggctcgcgca
     4981 gtggatcgac ttccagaata ggggtcagga ccctttttcg aaaaaagggt cctgaccacg
     5041 taatgcggct tcaccacttg acgatacgcg ctcaaagccg cgcaccctaa acgcctgcgg
     5101 agcgaactct actctcgagt cgtagcatca gcgcgtcgaa cgcattcatc tttgcgaccg
     5161 cttcaggcgc gcggggaatc agcgaccgga cttcggacga tgcgcgaaaa ggatgcgtgc
     5221 gccgccgata gaggtgacgc acgcgcacgt cgttgaggag ttccatgaag cgaagctgta
     5281 catacgagtg catgagatcc ttggtgtgcg caacacctga gctcgtacta cccaggtgtg
     5341 caatcgaatc ggcgtcgacg gactgcagaa agcgcacggt caaaccctct gtaagcgcca
     5401 gtagtagcag tacgtgaagg gtgtcgaaat agtcgtggta ctccttgaga tagacgccat
     5461 cccgtatgcc gatcgtcgcc agcggctctc gggccgtctg tggaagcttg cgataggctc
     5521 ttgcatcgac ccggtagcgc cgcatgatgg gtctaagaag cgtcgaattg aaatacaaga
     5581 agaacgtttc gggataaggg attcccaagc gcgcagcggt gttgccgaaa accgccacga
     5641 gacaatcgcg ttcgccgaat tcaaggtcgt cgaagatcga tgggtcgaag acgtagcagt
     5701 cgtgatcaag gaccccgaac gggcgttcgt tccatgccag gcacagcgtg agaacagttc
     5761 cgtgcgacag gcttgagccg ggaaaagtaa agagtttaaa gatcgggtat cgcgggaagg
     5821 cgcgcgctag atactggcac tcccattccg ctgccccgtt cgcgatcagg atcacccgca
     5881 tgtgcacggg tagcaacgac agacaaggaa ctaggaagtg aagggttcca ggcataacga
     5941 tcagatagaa atggccaccg gccacgggcg cgtgattgcg ctggaatgct tcaaatgccc
     6001 ggtgattaat cttatgaatt aggtcaaagt cgtccagatg gcgcgcgaag gccgagtagc
     6061 ctcgtcgaac gataacttcg ggcagaagaa gtagggctcg gccatgcgaa tggccgcgtg
     6121 cttctatcct gcgcaatgca ccaagaagcc ggcggatgtt ttgtctaagc atgtcgagca
     6181 tgccttgtga ctcaacggtg tcggtgtcca gcgagcggag ccctcgtact ttacctcgcg
     6241 agttacccgc gtcgatgctt cgcgctgcgg agcttttttg ccctgggggt aacgcgcaaa
     6301 tgcctagatc gcgccacgaa gctaggtgag tcggatcaga cctcccctag ttgaagtagt
     6361 gtagccagct gcgtagccgg cgcgcttatt gcatcgtgcc atcctactaa atcatgacct
     6421 tgcgcttggg ggttgacgtc ggcgggacga agatcgaggt cgtggtgctc gacgccaccg
     6481 gcgccgagcg catgcgtcgt cgcacgcact atccgagccg cgattaccaa agcgcgctcg
     6541 actgcctcgt cgagtcgatc cgcgcggcgc aagacgagtg tgagcaatgc tccatcggcg
     6601 tcggcctgcc gggtttcatc gaccgcgaga cctggttcct cgagaacgcg ttcaacacgc
     6661 cgtacaacaa gcagccgctc aagcgcgatc tcgagaaagc gctcggcaag gaagtacgct
     6721 tcgcgaacga cgcgaactgc tttgcgctat cggaggcggt cgatggggcg gcgcgcggcg
     6781 cgcgcgtcgt gttcggcgcc attctcggca ccggggccgg cggcggtttg gtggtgaacg
     6841 gtcaggtgat cgagggccgc catggcaccg ccggtgaatg gggacacacg ccgctgccgt
     6901 ggatgcgcca agacgagtac ccgggtccgc gctgcacttg cggacgcatc ggctgcatcg
     6961 agcagttcgt ttccggcccg gcaaagaatc gcgagcgcga cacgatggga gaggaagcgg
     7021 cgatgacgcg cttcgtcgat cgccttgcgc gcgcgttcgc catgatgatc aacatcgtcg
     7081 accctgacgt gatcgtggtg ggcgggggca tgtccaagca cgacgagatc ta
//
LOCUS       DASTPD010000208         6318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1330374, whole genome shotgun sequence.
ACCESSION   DASTPD010000208 DASTPD010000000
VERSION     DASTPD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330374"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFR57_06855"
     CDS             complement(<1..801)
                     /locus_tag="VFR57_06855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEU0259115.1"
                     /translation="MLDPARIMERCDALARHSELPGGLTRVFLSPEARAASDKVLGWM
                     REAGMEARLDDIGNAAGRYEGERPGSPCLMLGSHLDTVRDAGRYDGMLGVVTAIECVD
                     FLNKKNKRLPFAIEVIGFGDEEGVRFGTTLLGSRALAGIFDERALEAKDQSGKAMRDA
                     LREFGLDPARIPEVARKKGEVLAYAELHIEQGPVLEAEGLPVGVVIAINGFSRLRATL
                     RGAAGHAGTVPMRLRRDALAGAAECVLATERIVKGHAELVGTVGRIEAK"
     gene            825..1316
                     /gene="uraD"
                     /locus_tag="VFR57_06860"
     CDS             825..1316
                     /gene="uraD"
                     /locus_tag="VFR57_06860"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU0259116.1"
                     /translation="MALEEVNALGKEEFVARFGGVFERSPWIAERAWYKRPFRSIDEL
                     HRAMLDALASHEEKLELACAHPELAGAEALTVASDSEQARLGFNSLSRKELEEMNRLN
                     RRYRDKFGFPCIVALRLHADRRSVVTEMKCRLANDADTELRNALAQIGHITRGRLEKI
                     FNG"
     gene            1309..1641
                     /gene="uraH"
                     /locus_tag="VFR57_06865"
     CDS             1309..1641
                     /gene="uraH"
                     /locus_tag="VFR57_06865"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU0259117.1"
                     /translation="MGRLTTHVLDTANGKPGAGIAIDVAMLQAGSWKTLKTVKTNVDG
                     RTDAPVLEGDSFRAGAYELVFHVGSYFKGQGFLDLVPVRFVIMDPAAHYHVPLLCSPW
                     GYTTYRGS"
     gene            1647..2603
                     /locus_tag="VFR57_06870"
     CDS             1647..2603
                     /locus_tag="VFR57_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259118.1"
                     /translation="MARALLLAALLVPAMLQAQSWPAKPVHVIVTFPPGGSSDIVARL
                     LAAPLQSELGQPIIVDNRPGAGGTIGALEAARAAPDGYTLLLSNSAPISISPAMQDQP
                     RYDPVNSFAHVSYIGSVANVFVVHPSVPAKSLRELVIWIKQQPNPVNYGSGGIGSIGH
                     IVGETLKKEQGLKMEHVGYKGSSPMHNDLLGGTIRLAIDTLPQNVPFMKDGKLRALAV
                     TSPARAPMAPDVPSVLELGQKNLVAENFLGISGPARLPAPVIERLHAATRKALANPTV
                     QQRLADLGVQGRDMTPQEFSAFVANQVKEWYQPVKNSGAKLN"
     gene            2605..2940
                     /locus_tag="VFR57_06875"
     CDS             2605..2940
                     /locus_tag="VFR57_06875"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU0259119.1"
                     /translation="MPGISIHVVDVSRGIVASGMKIELYGPARRLIVSGVANAKGLLE
                     VDATVPAGAYEAVFHVGPWYREHRVSLPEVPFLDEVHYHFGISDPKQHYHLPFKCTPW
                     GYSCFRGGA"
     gene            2952..4103
                     /locus_tag="VFR57_06880"
     CDS             2952..4103
                     /locus_tag="VFR57_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU0259120.1"
                     /translation="MEAYLLDWLTLVARWAHIVVAIAWIGASFYFIWLDNHLESRKSE
                     EFVGEVHAIHGGGFYRAQKYRLAPAELPKTLHWFKWEAYWTWITGFALLVLVYYLNPE
                     LYLVDPAVMKLSASAAIGIGLTSLVVALAVYEGLCRSPLARNEWVFSITLSLFLAGTA
                     WALTQVFSGRGAFLHFGAILGTIMAGNVAHVIIPGQRELVRAKREGRRPDERYAVRGK
                     QRSVHNTYFTLPVILTMISGHHAMTFGQSWNWLVLIALTLAGALVRLWFVARHKGQAP
                     AWALGVALLAVAVVVVMLAPRPQTVSQPVDPAEVKRVIELRCANCHADTPTFQGFVEA
                     PRGVKLDSDERIRAQAPLIRQTVRARTMPPGNLTGLTEEERALIERWNP"
     gene            4091..5458
                     /locus_tag="VFR57_06885"
     CDS             4091..5458
                     /locus_tag="VFR57_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0259121.1"
                     /translation="MESLIQLSCARLAAAIRERRTTCIAAMEAVLERAHAVQPRLNCF
                     LRIDEDSALAAAHLADREIARGYVRGPLHGVPMAHKDMYYRRGVVSTCGSKILRDIPA
                     KATARVLEHLDAAGAIQFGVLNMAEFAMGPTGHNWHYGHCRNPWDPERITGGSSSGSG
                     AAVAARAAFAALGSDTGGSVRLPAAFCGIAGVRPTHSRVSVENILPLCPSLDTVGPLT
                     RTVDDAALMLESIAPGFACDLEKPIAGLRIGRPRNVFYEGCDAEIAAAMEKSLDVFRR
                     LGASVVDVELPDFAKLSALAGVLHAAEANPFHDEWFRTRPHDYSAQVRERLERGRPTH
                     ALDYVKALQARAPAITAFCEAVFSRVDLVHGPVVSFQTPTITETDISTGETAAQMLGR
                     FVRLTRPISYLGLPVVVANAGFTRAGMPIGMQLIAPPLDEATALRAGHAFQRSTDWHV
                     RVPPG"
     gene            5537..>6318
                     /locus_tag="VFR57_06890"
     CDS             5537..>6318
                     /locus_tag="VFR57_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259122.1"
                     /translation="MRLAALLAAALFSGVALAQKDVAVRFALDWRFEGPAAPYFVAID
                     KGYYKAEGLNVSIDPGAGSVEGINRVASGAYEMGFADINSLVKYRDNPRNLPVKAVMM
                     VYDTPAFSIVTLKKNGIAKPKDLEGKVLGAPAPDGAYAQWPIFVQANKIDASKVKIEN
                     IGFPVREPMLAQGKVDAITGFWFSSYMNLKANGVKDDDIVVLHMRDYGVDLYGNVIIA
                     NPDFMRFSPKAVAGFVKATIRGIQDTIKSPETAIDSLMKRNP"
BASE COUNT         1053 a         2081 c         2052 g         1132 t
ORIGIN      
        1 cttcgcctcg atgcgcccga ccgtgccgac gagctcggca tgccctttga cgattctctc
       61 tgtggcaagg acgcattccg cagcgccggc gagcgcgtcg cgccgcaggc gcatcggcac
      121 cgtgcccgcg tgccccgccg caccacgcag cgtcgcgcgc aggcgcgaaa agccgttgat
      181 cgcgatgacg acgccgaccg gcaggccctc ggcctcgagc accggcccct gctctatgtg
      241 cagctcagcg tacgcgagca cctcgccctt cttgcgcgcg acttccggta tccgcgccgg
      301 atcgagcccg aactcgcgta gcgcgtcccg catggctttt ccgctctgat cttttgcctc
      361 gagcgccctt tcatcgaaaa tccctgctag cgcccggctg ccgagcagag tcgtgccgaa
      421 gcgcacgcct tcctcgtcac cgaagccgat gacttcgatc gcgaacggca gtctcttatt
      481 cttcttattc agaaaatcaa cgcattcgat cgcggtgacc acgccgagca tgccgtcgta
      541 gcgcccggcg tcgcgcaccg tgtcgagatg cgagccgagc atgaggcagg gagagccggg
      601 gcgctcgcct tcgtagcgtc cggcggcgtt gccgatgtcg tcgagcctcg cctccatgcc
      661 ggcttcgcgc atccagccga gcaccttgtc gctcgccgcg cgcgcttcgg gtgaaaggaa
      721 cactcgggtg agtcccccgg gaagctctga atgccgcgcg agcgcatcgc agcgctccat
      781 gatgcgcgcg gggtcgagca tgcgccgagt gtacactagg gcgcgtggcg ctcgaggagg
      841 tgaacgcgct cggaaaagag gagttcgtcg caagattcgg cggcgtgttc gagcgctcgc
      901 cgtggatcgc ggagcgcgcg tggtacaagc ggccttttcg ctccatcgac gagctgcatc
      961 gcgcgatgct cgacgcgctg gcctcccatg aagagaagct cgagcttgcg tgcgcgcacc
     1021 ctgagcttgc gggggcagaa gcgcttacgg ttgcttcgga cagcgagcag gcgcggctcg
     1081 gattcaattc gctttcgagg aaggagctcg aagagatgaa ccggctgaac cgtcgctacc
     1141 gcgacaagtt cggctttccg tgcatcgtcg cgctcaggct tcatgccgac cggcgaagcg
     1201 tggtgaccga gatgaagtgc cgactcgcga atgacgccga taccgaactg cgcaacgcgc
     1261 tcgcgcagat cggccacatc actcgcgggc ggctggagaa gatcttcaat gggtaggctc
     1321 accacgcacg tgctcgatac ggcgaacggc aagcccggcg ccgggatcgc catcgatgtg
     1381 gcgatgctgc aggctgggag ctggaagacg ctcaagaccg tgaagaccaa cgtcgatgga
     1441 cgaacggatg ccccggttct cgaaggcgac agcttccgcg ccggcgccta cgagctcgtg
     1501 ttccatgtcg gcagctactt caagggccaa gggttcctgg atctcgtgcc ggtacgcttc
     1561 gtgatcatgg acccggccgc gcactaccat gtgccgctgc tttgctcacc gtggggttac
     1621 acaacttaca gagggagcta gccatgatgg ctcgcgcgtt actgctcgcc gcgcttctcg
     1681 tccccgcaat gcttcaggcc cagagctggc cggcgaagcc ggtccacgtg atcgtcacct
     1741 tcccgccggg cgggtcttcc gacatcgtgg cgcggctcct tgctgcgccg ctgcaatcgg
     1801 agctcggcca gccgatcatc gtcgacaaca gacctggcgc tggcggcacg atcggcgccc
     1861 tcgaagcggc acgcgccgcg cccgacggct atacgctgct gctctcgaac agcgcgccga
     1921 tcagcatctc gcccgcgatg caggaccagc cgcgctacga cccggtgaac agctttgcgc
     1981 atgtgtcgta catcggctcg gttgccaacg ttttcgttgt gcatccgtcg gtgcccgcca
     2041 agtcgctgcg cgagctcgtc atctggatca agcagcagcc caacccggtg aactacggtt
     2101 ccggcggtat cggctctatc ggccacatcg tcggcgagac gctgaagaag gagcagggcc
     2161 tgaaaatgga gcacgtcggc tacaagggat cctcgccgat gcacaacgat ctgctcggcg
     2221 ggacgattcg ccttgccatc gacacgctgc cgcagaacgt tccgttcatg aaagacggca
     2281 agctacgcgc gctcgcggtc acgtctccgg cgcgcgcgcc catggcgccc gacgtgccga
     2341 gcgtgctcga gctcggccag aagaacctcg tcgccgagaa cttcctcggc atctccggcc
     2401 cggcccggct gccggcgccg gtgatcgagc ggctgcacgc ggcgaccagg aaggcgctcg
     2461 ccaatcccac cgttcagcag cgcctggctg acctcggagt ccaggggcgc gacatgacgc
     2521 cgcaggaatt cagcgccttt gtcgcaaacc aggtgaagga gtggtaccag ccggtgaaaa
     2581 attccggcgc gaagctcaac tgaagtgccg gggatctcga ttcacgtggt cgacgtctca
     2641 cgcggcatcg tggcttcggg aatgaagatc gagctctacg gccccgcgcg caggctgatc
     2701 gtctcgggtg tcgctaacgc gaaagggctt ctggaagtcg acgccacggt gccggccggc
     2761 gcatacgagg cggtgtttca tgtcggcccc tggtaccgcg agcaccgggt atctttgccc
     2821 gaggtaccgt ttctcgacga ggttcactac cacttcggga tttcggaccc aaagcagcat
     2881 taccacctgc cgttcaagtg cacgccttgg ggctattcct gcttccgcgg cggcgcatga
     2941 acgcggctta gttggaagcc tatcttctcg actggctgac ccttgtcgcc cgctgggcgc
     3001 acatcgtggt cgccatcgcg tggatcggcg cgtccttcta cttcatctgg ctcgacaacc
     3061 atctcgagtc gcgcaagagc gaggaatttg ttggagaggt tcatgccatt cacggcggcg
     3121 gcttctaccg cgcgcagaag taccgcctcg cgccggccga gctgccgaag acgctgcatt
     3181 ggttcaagtg ggaggcgtac tggacgtgga tcacgggctt cgcgcttctg gtgctggtct
     3241 attacctcaa ccccgagctc tacctcgtcg acccggcggt gatgaagctc tcggcgagcg
     3301 ccgcgatcgg catcggcctc acaagcctgg tggtcgcgct tgctgtttat gagggcctat
     3361 gccgctcgcc gctggcgagg aacgagtggg ttttctcaat aaccctttct ctctttcttg
     3421 caggaacggc gtgggccctg acgcaggtct tcagcgggcg cggtgcgttc ctgcacttcg
     3481 gcgcgatcct cggcacgatc atggcaggca acgtcgcgca cgtgatcatc ccgggtcagc
     3541 gcgaactggt gcgcgcgaag cgggaagggc gccgtcccga cgagcggtat gccgttcgcg
     3601 gaaagcagcg cagcgtccac aacacttact tcacgctgcc agtcatcctc accatgatct
     3661 ccgggcacca cgcgatgacc ttcggccaat cgtggaactg gctggtgctg atcgcgctca
     3721 cgctcgccgg ggcgctggtg cgcctgtggt tcgtcgcccg gcacaagggg caggcgccgg
     3781 cgtgggcgtt gggcgtcgcc ttgctggccg tcgcggtcgt ggtggtgatg ctcgcgccgc
     3841 ggccgcaaac ggtttcgcaa cccgtcgatc cagccgaagt gaagcgggtc atcgagctgc
     3901 ggtgcgccaa ctgccatgcc gacacgccga ctttccaggg cttcgtggag gcgccgagag
     3961 gcgtcaagct cgacagcgac gagcgcatcc gcgcgcaagc tccgctcatc cgacagacgg
     4021 tgcgtgcgcg taccatgccg cctggaaatt tgacgggcct caccgaagaa gagcgtgctt
     4081 tgatagaacg atggaatccc tgatccagct ttcctgcgca cggctcgcgg ctgcgatccg
     4141 cgagcggcgc actacttgta tcgcggcgat ggaggcggtg cttgagcgcg cgcacgcggt
     4201 ccagccgcgc ctcaactgct tcctgcgcat cgacgaggac agcgcgcttg ccgcggcgca
     4261 tcttgccgac cgggagatcg cgcgcggcta cgtgcgcggc ccgctgcacg gcgtgccgat
     4321 ggcgcacaag gacatgtact atcgccgcgg cgtggtctcg acctgtggct cgaagatcct
     4381 gcgcgatatt cccgccaagg cgactgcgag agtgctcgag catctcgacg ccgcgggcgc
     4441 gatccagttc ggcgtgctca acatggcgga attcgcaatg ggtcccacgg gccataactg
     4501 gcactacggc cattgccgca atccctggga ccccgagcgc atcacgggcg gatcctcgtc
     4561 gggctcgggc gcagcggtgg cggcacgcgc cgcgttcgcg gctctcggct ccgacaccgg
     4621 cggctcggtg cggcttcccg ccgcgttctg cggcatcgcg ggcgtgcgcc cgacgcacag
     4681 ccgggtatcg gtggagaaca tcctgccgct ttgcccgtca ctcgatactg tcggcccgct
     4741 cacccgcacg gtggacgacg ctgcgctaat gctcgaatcc attgccccgg ggttcgcgtg
     4801 cgatctggaa aaaccgattg ccggattgcg gatcggccgg ccgcgcaacg tgttctacga
     4861 aggctgcgat gccgagattg cggcggcgat ggagaagtcg ctcgacgtgt ttcgccgcct
     4921 gggcgcttca gtcgtggacg tggagttgcc ggatttcgcg aaattgagcg cgctcgccgg
     4981 ggtgctgcac gccgcggaag cgaatccgtt ccacgatgaa tggttccgca cccgcccgca
     5041 cgactattcg gctcaggtgc gcgagcggct ggagagaggc cggccgacgc acgcgctcga
     5101 ttatgtgaag gcgctgcagg cacgcgctcc ggcgatcacg gcgttctgcg aggcggtgtt
     5161 ctcgagggtg gatctcgtgc acgggccggt ggtgtcgttc cagacgccga cgatcaccga
     5221 gaccgacatc tcgaccggcg agaccgctgc gcaaatgcta gggcgcttcg tgcgccttac
     5281 gcgcccaatc agctacctcg gcctgcctgt ggttgtcgcc aacgccggct ttacccgggc
     5341 cggcatgccg atcggcatgc agctgatcgc cccgccgctc gatgaggcga ccgcgctgcg
     5401 cgcgggccac gcctttcagc ggtccaccga ctggcacgtg cgcgttccgc ctggataggg
     5461 cgctcagaat ccatcgcctt cagtgcaggc ctgatttggc attagacttc ccttgccatc
     5521 caatcggggg aactctatgc gccttgccgc tctgctcgct gccgcgctct tttccggtgt
     5581 ggcgctcgcc cagaaagacg tcgcggtccg cttcgcgctc gactggcgtt tcgaagggcc
     5641 ggcggcgccg tatttcgtcg cgatcgacaa gggctactac aaagccgagg gactcaacgt
     5701 ctcgatcgat ccgggggcgg gttcggtcga gggcatcaac cgagtcgcct cgggtgctta
     5761 tgaaatgggc ttcgccgaca tcaactcgct ggtgaagtac cgcgacaacc cgcgcaacct
     5821 gccggtgaag gcggtgatga tggtgtacga cacgccggcg ttctccatcg tcacgctgaa
     5881 gaaaaacggc atcgcgaagc ccaaggacct ggaaggcaag gtgctcggcg ctccggcgcc
     5941 cgacggcgcg tacgcgcagt ggccgatctt cgtgcaggcg aacaagatcg acgcctccaa
     6001 ggtgaagatc gagaacatcg gcttcccggt gcgcgagccg atgctcgcgc agggcaaggt
     6061 cgatgcgatc accgggttct ggttctcctc ctacatgaac ctgaaggcga acggcgtgaa
     6121 ggacgacgac atcgtggtgc ttcacatgcg tgactacggg gtcgacctct acggcaacgt
     6181 gatcatcgcc aaccccgatt tcatgcgttt ctcgccgaag gcggtggcgg ggttcgtcaa
     6241 ggccacgatc cgcggcatcc aggacacgat caagagcccg gagaccgcga tcgactcgct
     6301 catgaagcgc aatccgat
//
LOCUS       DASTPD010000209        23479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_309561, whole genome shotgun sequence.
ACCESSION   DASTPD010000209 DASTPD010000000
VERSION     DASTPD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309561"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..829)
                     /locus_tag="VFR57_06895"
     CDS             complement(74..829)
                     /locus_tag="VFR57_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0259123.1"
                     /translation="MDHRTKVLSLAVVIAGAGAAAWFLYLEEMVFGSDAPPVASAPTR
                     KAAPAPKPVALAPVAATAPQAEAVKSAPAPEPKVTPAVLTQPAEAPKAIAPVETVAAP
                     QSAEKPAKAEAPKAEEKPKPVARKPRPTPAAPAAAAAPKEVAIRETPAYTATRDSGPR
                     FNDLTTAVLYRDPKTVQELLTLGKWADKPDSRGVTPLMDAAQLGDAESAEMLLRAGAD
                     PNRPGPGGTTAMSIARERKDSGLTGLLGKHGGR"
     gene            complement(858..2189)
                     /locus_tag="VFR57_06900"
     CDS             complement(858..2189)
                     /locus_tag="VFR57_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0259124.1"
                     /translation="MNKRAEFVATNLEQAAFWMPFTANRQFKKAPRLLARAEGMYYWT
                     HDGRQVLDGTSGLWCVNAGHCRPKIVEAVKRQVAEMDFAPTFQMGHPVAFEFAERLAQ
                     IAPQGFSRVFFTNSGSESADTALKIALAYHRARGEPGRFRLIGRERGYHGVNFGGMAV
                     GGITANKKVFGPGVAGVDHIRHTHDLARNAFSRGQPKHGAEFADELERLCALHDPSTI
                     AALIVEPVAGSAGVLVPPVGYLERLREICTRHGILLIFDEVITGFGRLGAPFGSQHFN
                     VMPDLFTVAKGITNATVPMGAVFVKDSIFQPFMQGPEGIELFHGYTYSGHPLACAAGI
                     GTLDTYEEEGLLTRGAALAKPWEDALYSLKGGPHVIDIRNCGLMAAIELEPRAGQPGR
                     RAYEVFLKAFEAGVLIRVTGDTIALSPPLIVERQQIDRIVSCVGEMLRGVP"
     gene            complement(2209..3057)
                     /locus_tag="VFR57_06905"
     CDS             complement(2209..3057)
                     /locus_tag="VFR57_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0259125.1"
                     /translation="MKLCRYGKAGYEKPGVIDREGRLRDLSKVVDNLGPNEISPRGLK
                     MLTRLKPETLPPVNSNPRFGVPYVGIGKFIAIGLNYSDHAKEAGMPIPSEPIVFMKAT
                     TCINGPNDDVIQPKNSTKLDWEVELGVVIGSQAKYVSEAEALNHVAGYCVINDVSERS
                     FQMATTQWDKGKGFDTAGPIGPYLVTTDEIADPQTLDMWLDVNGKRMQSGNTRTMIFG
                     VATIISHVSQYMTLLPGDIICTGTPPGVGAGIKPNPVFLKPGDVMTLGIQNLGEQRQK
                     VVAYSA"
     gene            complement(3054..3797)
                     /locus_tag="VFR57_06910"
     CDS             complement(3054..3797)
                     /locus_tag="VFR57_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0259126.1"
                     /translation="MAGPRLAGKTAFVTAAGQGIGRAAALAFAREGAKVWATDLNAKT
                     IGELEGKDSIRTRALDVTDEAAINKTASEVGDIDVLFNCAGIVHHGSILDASVKDWDQ
                     AFAVNVKSMFLVARAFIPGMLKKGRGSILNMASVASSMRGLPNRFVYGASKAAVIGLT
                     KSIAADFVGKGIRCNAICPGTVDTPSLQGRINAFADPVQARKDFVARQPMGRLGTVDD
                     ITGILVFLASDEALFATGNAYPVDGGMTI"
     gene            3827..4516
                     /locus_tag="VFR57_06915"
     CDS             3827..4516
                     /locus_tag="VFR57_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259127.1"
                     /translation="MNRLDFAGRNAIVTGGAQGIGAAIVKRLQASGASVMIWDLDGSP
                     RVDVSDPKSVEKAAASIAKVDILINNAGIAGMNKPTVDYPIEEWERVLRVNLTSQFLC
                     CRAVAPLMVKAGYGRIVNIASIAGKEGNPNAVAYSASKAGVISLTKSLGKELAQTGVL
                     VNCVTPAAAKTAIFDQMTKQHIDYMLSKIPMNRFVTVDEVAALVCWLASEDCSFSTGA
                     VFDVSGGRATY"
     gene            4517..6226
                     /locus_tag="VFR57_06920"
     CDS             4517..6226
                     /locus_tag="VFR57_06920"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEU0259128.1"
                     /translation="MKKRSIQWFGRQDIYGFIYRSWVKNRGVPQDQFDGRPVIGICNT
                     WSELTPCNTHFRVLAEHVRNGILEAGGFPLEFPVMSLGETLMRPTAMLYRNLAAMSVE
                     ESIRANPLDGVVLLAGCDKTTPATLMGAASVDLPTILVSGGPMLSGKYRGTEIGSGTN
                     TWSMSEDLRAGKITLDEFHEAESCMHRSHGHCMTMGTASTMASMSEALGVAMPGNAAI
                     PAVDARRNVLARLAGRRVVEMVKENLVLSKILVRPAFENAIRALAAIGGSTNAVIHLV
                     AVARRVGIELSLDDFDGLGRGVHTLVNLMPSGKYLMEDFYYAGGLPAVLREIGPLLHK
                     DALTVNGKTMWENVKDAPCFNREVITPFAQPFKENTGIAVLRGNLCPDGAIIKPSAAT
                     PKLMQHRGRAVVFENIEDFHKRIDDPNLDVDERSVMVLKNCGPKGYPGMAEVGNMPLP
                     PKLLKKGITDMVRISDARMSGTAYGTVVLHMSPEAAAGGMLALVENGDEIELDVAKRK
                     LELLVNERELTERKAGWKAPQPPLERGYWKLYYDHVNQAHEGADLDFLVGKSGPFVPR
                     DNH"
     gene            6213..6839
                     /locus_tag="VFR57_06925"
     CDS             6213..6839
                     /locus_tag="VFR57_06925"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0259129.1"
                     /translation="MITTEFALYCFAQSGNAYKAALMLQLCGATWQPRFVDFFGGETR
                     TPRYREINEMGEAPVLEHGAKRLSQSGVILHYLADHFGKFQGHDKLEVLRWILWDNHK
                     LTSYIATLRWMVTLAKVGEPQVHEFLRGRVKGALGILDAHLGKSAFAVGDQPSIADIS
                     MCGYLYWPDEFGVSWTDYPRIGAWLERIRKLPGWVHPYELMPGHPLKR"
     gene            6842..8362
                     /locus_tag="VFR57_06930"
     CDS             6842..8362
                     /locus_tag="VFR57_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0259130.1"
                     /translation="MLQAVELTIRLHAEDDVVIARVDLPTGTLVSKENVRAAVTIPAG
                     HKLAVRDIAQGRPVRRYNQIIGFATRDIKAGEHVHVHNLGMGDFQRDYAFSSLKIETA
                     YITPAATFEGIVRADGRVATRNYIGILTSVNCSATAARMIARHFENNLKQYPNVDGVV
                     ALTHKSGCGMASEGEGMDVLRRTMAGYARHPNFFATQVIGLGCEANQINNLLSTHNLK
                     RHDRMHAYTIQEKGGTMKAVREGIARIEAILPEANKVKRETVPASHLILGLQCGGSDG
                     YSGISANPALGAAVDLLVRHGGTAILSETPENYGAEHLLTRRAVSQQVGEKLLARMRW
                     WEEYTRRNNMEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTTNLVDVYEYAEPVSAKG
                     FVYMDTPGFDPVSATGQVAGGATMICFTTGRGSAYGCKPAPSLKLATNTPLFVHQEED
                     MDFNCGTIIDGTESIEQAGARFFQLILDTASGKRSKSEEFGYGDDEFAPWIIGATM"
     gene            complement(8363..8845)
                     /locus_tag="VFR57_06935"
     CDS             complement(8363..8845)
                     /locus_tag="VFR57_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0259131.1"
                     /translation="MKAPMFELSGVDGKVYSPVTARGDKGLLVMFICNHCPYVKRVID
                     GIVRDCKGLAPLGIGSIAVMSNDPTDYPDDSFENMKRVAREKGFGFPYVIDHTQEVAR
                     AYGTVCTPEFFGFDAGLNLEYHGRYDEMFAAMKRIAETGKGPEKQIPSVGCSIKWSRN
                     "
     gene            8860..9585
                     /locus_tag="VFR57_06940"
     CDS             8860..9585
                     /locus_tag="VFR57_06940"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU0259132.1"
                     /translation="MVPRFYVDAPLRARSVSTLPEDAAHHAVHVLRLREGEPVVLFNG
                     RGGEFSARIASMKRLEISIDILEHRAIERESPLRVTLVQGISASDKMDGTVRKAVELG
                     VAEVQPVLAARSVARPKGERAENRRAHWQKVVIAACEQCGRNRIPQVHPVMGAMEYRA
                     PQEAAKLLLSPNSRLRFAQAVKTGETFALAAGPEAGFTADEEAAFASAGFVPVSLGPR
                     VLRTETAAVAAIAALNALRGDSA"
     gene            complement(9557..10138)
                     /locus_tag="VFR57_06945"
     CDS             complement(9557..10138)
                     /locus_tag="VFR57_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259133.1"
                     /translation="MKTIRILLAAMLIAPAAPALAQNEEWPAFGHALTLIHLFVRIAA
                     ESETPTQSLRAIDEVLLGRNPVANEAVSGLVKEMLADVPPQHRATIAAIGRDLAAAAR
                     KNLGVKDARPRMDPLQARRDLTSMGLTYHDSKQFLDAVKRNDAIAAELFIAGQGVDLG
                     ATDFWGRDAVDFARSNGNERLAELITRNRPATR"
     gene            10222..11538
                     /gene="argA"
                     /locus_tag="VFR57_06950"
     CDS             10222..11538
                     /gene="argA"
                     /locus_tag="VFR57_06950"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEU0259134.1"
                     /translation="MENAGSFVRWFRQVAPYVHDFGGRTFVIAFGGEMVAERARFQSF
                     IHDVNVLASLEIRLVLVHGARPQIEAEMKAKGMRSRYAQGLRITDEAALTVVKHAGGV
                     LRVEIEALLSQGLPDSPMARAQIRVVSGNYITARPIGVRNGVDFQFTGAVRKVDAAAL
                     AAQLDVVDIVVVPHLGYSSTGEVFNLAWEDVAESVAVALHADKLLMYTDKLPTSRKGE
                     VISELTAEEASALASARKSDLTPTARRAMEHLARAVENGVARGHLVSRRAPGSLLLEL
                     FTHAGVGTMITADAVEKLRPARVEDVRGMLALIEPLEAEGTLVKRSRELLEAEIGNFL
                     VVEHDARIVGCAALYPFPDDRSAEFACLAVDAEYREAGYGERLLKACEQRARGLKLRK
                     LFALTTHAAHWFLEQGFRAASVDALPERRKALYNWRRGSKVFLKRI"
     gene            11548..11826
                     /locus_tag="VFR57_06955"
     CDS             11548..11826
                     /locus_tag="VFR57_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEU0259135.1"
                     /translation="MARMVKCVKLGREAEGLEFPPYPGALGKRIWENVSKEAWQQWLK
                     QQTMLVNENRLNLADQRARKYLMEQTERHFFGAGADVASGYVPPEDKK"
     gene            complement(11799..12953)
                     /locus_tag="VFR57_06960"
     CDS             complement(11799..12953)
                     /locus_tag="VFR57_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259136.1"
                     /translation="MFLLSLAAFASAASLRATDPLLPLIADEYAVTAGAASAAVTAFA
                     LSYGLLQVVCGPLGDRYGRYRTIAAAALVSAFGSAACALAPSLGALVAARFVSGATIG
                     AFIPLALAWIGDTVSYEKRQPLLARFLVGQMAGVAFGTAAAGWLGEHFGWRTIFFAMA
                     ALLFIVALLLVLEVKRNPLAARRGAGRGAIRQSLARMPRLLVHPRLRVLIATGYVEGL
                     FIFGALAFVAIYLQRRFLLGPGLAGTLVTAYAAGGMLYALAARRAVRRLGERGLATLG
                     GGALFIGYLGLALSPFLTLTTLSIGAVGAGFYMLHTTVQTHVTQVAPEDRGSAVALFA
                     TFLFLGQASGVWLASHVVDGAGMTPVFLAAALGLAALATAFRYFLSSGGT"
     gene            complement(12962..13627)
                     /gene="rpiA"
                     /locus_tag="VFR57_06965"
     CDS             complement(12962..13627)
                     /gene="rpiA"
                     /locus_tag="VFR57_06965"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU0259137.1"
                     /translation="MTQDELKERVAREALKYVQDPVIGVGSGSTVFKFIEALASIKHQ
                     IDGAVAASETSAERLRKIGVRVLDLNSVNELPVYIDGADEVTEHLHMIKGGGGALTRE
                     KITAAVARRFVCICDGSKLVPVLGKFPVPIEVIPMARSYVAREVVKMGGHPRLREGFT
                     TDNGNVVLDCHGMTLLDPPKVEGELNQIAGVVTNGIFARRPADVLLLAEASGVLTKKR
                     LSS"
     gene            complement(13624..15336)
                     /gene="lpdA"
                     /locus_tag="VFR57_06970"
     CDS             complement(13624..15336)
                     /gene="lpdA"
                     /locus_tag="VFR57_06970"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0259138.1"
                     /translation="MPGTEVKVPDIGDFKEVEVIEVLVKPGDAIDKEQSLITLESDKA
                     TMEIPSPTAGKVAKLVVKVGDKVSKGSPILILDAEATRAEAPAMRAPPKAPEAPQSSS
                     PSPSAGADLECDVLVLGAGPGGYSAAFRAADLGMKTVLVERYATLGGVCLNVGCIPSK
                     ALLHTAAIVDGAKALASHGITFGEPKVDLAKLRGFKDKVVGKLTGGLAGMAKMRKVQV
                     VQGAGTFLDRNRMDVGGKTIRFQSAIIAAGSQAAKLPFLPDDPRIVDSTGALELRSIP
                     KKLLIIGGGIIGLEMGTVYSTLGARLDVVEMMDGLMLGADRDLVAVWQKFNAPRFDKI
                     MLKTKTTKVEGTKDGLKVSFEGAEAKTYDMILVSVGRVPNGRKIGAEKAGVEVDAKGF
                     IPVDSQRRTNVPNIFAIGDIAGNPMLAHKAVHEGHVAAEAAAGQKSHFDARVVPSVAY
                     TDPEIAWVGVTEDEAKKSDVAVGVAKFPWAASGRAIANARDEGFTKLIFDAETHRVVG
                     GGIVGLGAGDLISEIALAIEMGADATDIGKTIHPHPTTAESVGMAAEVFEGVCTDLPP
                     QRKR"
     gene            complement(15340..16923)
                     /gene="aceF"
                     /locus_tag="VFR57_06975"
     CDS             complement(15340..16923)
                     /gene="aceF"
                     /locus_tag="VFR57_06975"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEU0259139.1"
                     /translation="MNQVVVPDIGDFKEVEVIEVLVKPGDAVSKEQSLITLESDKATM
                     EIPSPGSGVVKELKIKVGDKVSKGSPILVLDEKGNGETAPEEKLKPPVAKAEAPKSQP
                     QQAGSAMTVPVPDIGDFKDVEVIEVLVKPGDAVTKEQSLITLESDKATMEIPSPAAGV
                     VKELKIKVGDRVSTGAPILVLDSKDAATAKPTAPPVAKPQQAPKPVAAAPAAAPREEA
                     PSSGAKPHASPSVRKFARELGVDLSRVQGSGPKGRILHTDVQAFVKGIVQGKAPVAPQ
                     GGGALPFNLPAWPEVDFAKFGPVETKALSRIQKLSGPYLHRNWISIPHVTQFDEADIT
                     DLEAFRKAQTVETEKKGFKLTMLAFMIKACVTALRQYPQFNSSLDKSGENLIVKKYYN
                     VGVAVDTPGGLVVPVVRDADRKGVFDIAQELGDISKKARDGKLAPADMQGGTFSISSL
                     GGIGGTAFTPIINAPEVAILGVSRSALRPVWDGKTFAPRLMLPLSLSYDHRVIDGATA
                     ARFSAFLVSVLSDIRKLIL"
     gene            complement(16920..19610)
                     /gene="aceE"
                     /locus_tag="VFR57_06980"
     CDS             complement(16920..19610)
                     /gene="aceE"
                     /locus_tag="VFR57_06980"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0259140.1"
                     /translation="MSAVPQFPAANDPDALETQEWLDALEAVLEREGPQRAHYLLERL
                     IDKARRSGAHIPYSPNTAYINTIPPHMEERSPGNIALEERIRSICRWNAMVMVAKANK
                     SDDELGGHIASFASVGTLFGTGQQHFWNAPHEGHGGDLVYFQGHSSPGVYARSLMEGR
                     LSADRLLSFRREVDGKGVSSYPHPWLMPDYWQFPTVSMGLGPIQGIYMARFLKYLHAR
                     GLAQTANRKVWVFCGDGEMDEPESMGAIGLAAREKLDNLIFVVNCNLQRLDGPVRGNG
                     KIIQELEGEFRGAGWNVIKVIWGSYWDPLIARDKEGILLRIMEETVDGEYQNYKANDG
                     AFVRKHFFGKHPKALELVARMSDEDIWRLNRGGHDPHKVYAAYAAAVKHKGQPTVILA
                     KTIKGYGLGKQGQAKNPTHQLKKIDISTIKEMRDQFAIPIPDDKVEELPFYIPPADSP
                     EMKYLNERRKALGGFVPQRREKAEKVPAVPELAAFDALLKGSGEGREISTTMAFVRVL
                     TALVRDKELGRHIVPIVPDEARTFGMEGMFRQLGIFAPEGQKYEPVDKDQVMYYREDK
                     AGQILEEGINEAGAFSSWIAAATSYSHSNRVMVPFYIFYSMFGFQRIGDLAWAAADQR
                     ARGFLLGATAGRTTLNGEGLQHEDGHSHILSSVIPNCLSYDPTYGYEVAVIIQDGLRR
                     MVTNQEDIYYYLSLMNENYEHPAMPQGVEEGILKGMYLLRESKAKHKQRVQLLGSGTI
                     LREVEAAAELLEKDWGIAADVWSATSFTELRRDGLAVDRWNMLHPEAKPKAPYVTQLL
                     EKRPAGPVIAASDYMKTFADQIRPFISSDRVYRVLGTDGFGRSDSRAKLRHFFEVNRF
                     FVVIAALKAFADQGEGKAKAVAEAIKKYGIDPEKSDPTTV"
     gene            19691..20557
                     /gene="folD"
                     /locus_tag="VFR57_06985"
     CDS             19691..20557
                     /gene="folD"
                     /locus_tag="VFR57_06985"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU0259141.1"
                     /translation="MAAQILDGKSLAASIRAALKERVAALVQRGVKPGLAVVLAGDDP
                     ASRVYVRNKRLAATETGVHSELIELPGTIGQRELRERIEGLNANRAIHGILVQLPLPK
                     PIDAAAVLEAIAPAKDVDGFHEINMGALFAGRPAVVPCTPAGVMRLMEQARIPLAGRH
                     AVVIGRSNIVGKPVAQLLLQKDATVTICHSKSSDLSELTRRADVLIAAVGRPKLVRRD
                     MVKPGACVIDVGVNRLEDGKLAGDVDFQAVKEVAGWITPVPGGVGPMTIAMLLENCIR
                     AASRELEPKPAG"
     gene            20520..22574
                     /locus_tag="VFR57_06990"
     CDS             20520..22574
                     /locus_tag="VFR57_06990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0259142.1"
                     /translation="MPHENLSQNPLVDFSGLPRFADIRPEHVTPAMELLLSDGRSTVN
                     AVVNARPTWHDFIEPLEDANERIGRAWGQVAHLHAVMDSPELREAYNSNLPKVTKYWT
                     ELGQNQELFEKYKALRAAPVFAALSAARKRIVENAIRDFRLSGAELAPEKKKRYAEIQ
                     EELARLSAKFSENVLDATNAFGICIAKEEAAGIPDDVLQAAREAAQKDGKQGFKFTLH
                     MPSYLPVMQYADNRALREKMYYASSTRASEFGKPEWDNAPLIARIVPLRREMAQLLGY
                     ANFAELSLVPKMADSPGQVLGFLDDLARRARAFAEKDVGELRDFAKAELGIAELQAWD
                     IAYASEKLRLKRYSFSDQEVKQYFPEEVAIRGLFRVAETLYGVKFVPAQAPTWHPDVR
                     FFEVRDASGKAIGQFYMDLYARETKRGGAWMDDAITRRKKRDHVQNPVAYLNCNFSRP
                     VGDKPALFTHDEVLTLFHEFGHGLHHLLTRVDDLGVSGINGVEWDAVELPSQFMENFC
                     WEWDVLREMTRHVDTGQPLPKVLYDRMLAAKNFQSGLAMLRQIEFAVFDMRLHYDFDP
                     ASGRSALDLLNEVRKQVAVLFPPGYNRFPNSFGHIFGGGYAAGYYSYKWAEVLSADAY
                     SLFEETGVLSPDAGARFREEILAVGGSRPAAESFRAFRGREPSVDALLRHNGMTTETA
                     AT"
     gene            22571..23341
                     /gene="xth"
                     /locus_tag="VFR57_06995"
     CDS             22571..23341
                     /gene="xth"
                     /locus_tag="VFR57_06995"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0259143.1"
                     /translation="MKLATWNVNSLKVRLPHLLDWLAAAQPDVMCLQETKTEDHNFPR
                     VEIERAGYRSVFCGQKAYNGVAILARDKLEDVQYGIPDFPDDPKRVIAATLRGTRILC
                     LYAPNGEAPRTDKYAYKLRWYTALTKWLGRKLEQYPRLAILGDLNIAPEPRDVHNPKR
                     WEGKIHFSEPERAAFREVIAAGVTDAFRLFEQPEKQFTWWDYRLKAFERGWGLRIDHI
                     LLAPALAAQCDACSIDVEPRKLERPSDHAPVIAELGAA"
BASE COUNT         4144 a         7673 c         7542 g         4120 t
ORIGIN      
        1 cgcggccgcc ggctgggcgt ggtgagagcg gccgcgaaat aagaacaaga aaggggcgcg
       61 gagccccttt ttttcagcgt cccccgtgtt tgcccaggag cccggtgagg ccggaatcct
      121 tgcgctcgcg cgcgatggac atcgcggtcg tcccgccggg ccccggccgg ttcggatccg
      181 caccggccct gagaagcatc tcggcgctct ccgcgtcacc gagctgcgcc gcgtccatga
      241 gcggcgtgac tcctcggcta tcgggcttgt cggcccactt gccgagcgtg agcaactctt
      301 gcacggtctt cgggtctcga tagagcacgg ccgtggtgag gtcgttgaag cgaggccctg
      361 aatcgcgcgt cgcggtgtag gccggcgttt cgcggatcgc cacttctttc ggtgcggccg
      421 ctgcggcggg cgcggcgggc gtgggccgag gcttgcgggc gaccggcttc ggcttctcct
      481 ctgccttcgg tgcttccgcc ttcgcaggct tctcggcgga ctggggcgca gcgaccgttt
      541 ctacaggagc tattgctttc ggcgcctctg cgggttgagt gagcacggcc ggcgtgacct
      601 tcggttctgg cgccggtgcg gatttcaccg cctcggcctg cggcgcggtg gcggcgaccg
      661 gtgcgagggc cactggcttc ggcgcaggcg cggctttgcg ggtcggggca cttgcgaccg
      721 gcggagcgtc ggagccgaaa accatttctt cgaggtacag gaaccaggct gcggcaccgg
      781 ctccggcaat caccactgcg agactgagaa ccttggtgcg atggtccatc tttgttctcc
      841 cttcccccta cttgttctta tggcaccccc cgcagcatct ctcccacgca gctgacgatc
      901 cgatcgatct gctggcgctc cacgatgagc gggggcgaca gtgcgatggt atcgccggtg
      961 acgcgaatca acacgcctgc ttcgaatgct ttgagaaata cttcatacgc gcgccggcct
     1021 ggctggcccg cacgcggctc cagctcgatc gccgccatta ggccgcagtt gcggatgtcg
     1081 atgacatgcg gcccaccttt cagggaatac agcgcatctt cccacggctt ggccaaggct
     1141 gcgccccgag taagcaaacc ttcttcctca taggtgtcaa gcgtgccgat ccccgccgcg
     1201 cacgcgagcg gatgaccgga ataggtgtag ccgtggaaga gctcgatacc ttccgggccc
     1261 tgcataaacg gctgaaagat gctgtccttc acgaacaccg cgcccatcgg cacggtggcg
     1321 tttgtgattc ccttcgccac ggtgaagagg tccggcatca cgttgaagtg ctgcgagcca
     1381 aacggcgccc ccagacggcc gaacccggtg atcacttcgt cgaagatgag aaggatgccg
     1441 tgccgcgtgc agatctcgcg cagccgctcg aggtagccaa ccggcggcac cagcacgccg
     1501 gcggagccgg cgaccggctc gacgatcaag gcggcgatgg tcgacggatc gtgcagcgcg
     1561 cacagccgct cgagctcgtc ggcgaactcg gcgccgtgct tcggctgacc gcgcgagaac
     1621 gcattgcgcg cgagatcgtg cgtgtggcgg atgtggtcga cgcccgccac gcccggcccg
     1681 aataccttct tgttggcggt aatgccgccg accgccatcc cgccgaagtt aacgccgtga
     1741 tagccgcgct cgcgcccgat gagccggaag cgccccggct cgccgcgcgc acgatggtag
     1801 gcgagcgcga tcttgagcgc ggtatcggcc gactcggacc cggaattggt aaagaaaacg
     1861 cgcgagaagc cctgcggtgc gatctgagcg agtcgctcgg cgaactcgaa tgcgaccggg
     1921 tggcccatct ggaaagtagg cgcgaaatcc atctcggcga cctggcgctt gaccgcctcg
     1981 acgatcttcg gccggcaatg gccggcgttg acgcaccaca ggcccgaagt gccgtcgagc
     2041 acctgccgcc catcgtgggt ccagtagtac atgccctcgg cgcgagcgag gagacgcggc
     2101 gccttcttga actgccggtt cgcagtaaac ggcatccaga atgccgcctg ctcgaggttg
     2161 gttgctacga actccgcacg cttgttcatg cgggcaacat tactccttct atgcggagta
     2221 ggcgaccacc ttctggcgtt gttcgccgag gttctggatg ccaagcgtca tcacatcgcc
     2281 gggcttgagg aacacggggt tcggcttgat gcccgcgccg acgccaggcg gagtgccggt
     2341 gcagatgatg tcacccggca gcagcgtcat gtactggctc acgtgcgaga tgatcgtcgc
     2401 cacgccgaag atcatggtgc gcgtattgcc gctttgcatg cgcttgccgt tcacgtcgag
     2461 ccacatgtcg agcgtctgcg gatcggcaat ctcatccgta gtcacgagat aggggccgat
     2521 cggcccggcg gtgtcgaagc ccttgccctt gtcccactgc gtggtggcca tctggaacga
     2581 gcgctcggag acgtcgttga tgacgcagta gccggccacg tggttgagcg cctcggcttc
     2641 gcttacgtat ttggcctgcg agccgatgac cacgccgagc tccacctccc agtcgagctt
     2701 ggtggagttc ttgggctgga tcacgtcgtc attcggcccg ttaatgcagg tggtcgcctt
     2761 catgaagacg atcggctccg atggaatcgg cataccggcc tctttggcgt gatccgagta
     2821 gttcaagccg atggcgatga acttgccgat gccgacgtaa ggcacgccaa agcgcggatt
     2881 ggagttgacc ggtggcagcg tctcgggctt caggcgcgtg agcatcttca ggccgcgagg
     2941 cgagatctcg ttcgggccga ggttgtcgac gaccttcgaa aggtcgcgca gcctgccctc
     3001 gcggtcgatc acgccgggtt tctcgtagcc cgctttcccg tagcggcata gtttcatatc
     3061 gtcattcccc catcgactgg ataagcgtta ccggtcgcga acagtgcctc gtccgaggcg
     3121 aggaacacca ggatgccggt gatatcgtcc accgtgccga ggcgtcccat cggctggcgc
     3181 gcgacgaagt ccttgcgcgc ctgtactgga tcggcgaatg cgttgatgcg tccctgcagc
     3241 gaaggggtgt cgaccgtgcc ggggcaaatg gcgttgcagc ggatcccctt gccgacgaag
     3301 tcggcggcaa tcgacttggt gagcccgatc accgcggctt tgctcgcgcc gtaaacgaag
     3361 cgattcggca gcccgcgcat cgaagaggcc accgaagcca tgttgaggat cgagccgcgc
     3421 cccttcttca gcatccctgg tatgaaggcg cgcgcgacca gaaacatcga cttcacgttc
     3481 accgcgaacg cctgatccca gtccttgacg ctcgcatcca ggatcgagcc gtggtgcacg
     3541 atccccgcgc agttgaagag cacgtcgata tcgccgactt cgcttgcggt tttgttgatc
     3601 gcggcttcgt cggtgacgtc gagcgcacgg gtgcggatgc tgtccttgcc ctcgagctcg
     3661 ccgatcgtct tggcattcag gtcggtcgcc cagaccttcg cgccttccct ggcgaaggcg
     3721 agcgccgcgg cccggccgat gccttgcccg gcggcggtga cgaacgcggt tttgcctgcg
     3781 agtcggggtc ctgccatgga aggcggcgat tatacttcgc gttcccatga atcgactcga
     3841 tttcgcgggt cggaacgcga tcgtgaccgg cggtgcgcaa ggtatcggcg cggcgatcgt
     3901 gaaacgcctg caagcctccg gcgcaagcgt gatgatctgg gatctcgacg gctcaccgcg
     3961 cgtcgatgtt tcggatccga agtccgttga gaaagcggcc gcgtcgatcg ctaaagtcga
     4021 catcctgatc aacaacgccg gcatcgccgg catgaacaaa cccaccgtcg attatccgat
     4081 tgaggaatgg gaacgcgtgc tgcgggtgaa cttgaccagc cagttcctct gctgccgcgc
     4141 ggtcgcgccg ctcatggtga aggccggata tgggcgcatc gtgaatatcg cctcgatcgc
     4201 cggcaaggaa ggcaatccaa acgccgtcgc gtattccgcc tccaaagcgg gtgtgatttc
     4261 gctgacgaag agcctcggca aggagctcgc gcagaccggc gtgctggtga actgcgtgac
     4321 gcccgccgcg gcgaagacgg ccatctttga ccagatgacc aagcagcaca tcgattacat
     4381 gctctccaag atcccgatga accgcttcgt caccgtagac gaggtcgctg cgcttgtgtg
     4441 ctggctcgcg agcgaggact gctcgttctc gaccggcgcg gtcttcgacg tctccggcgg
     4501 acgcgccacc tactgaatga aaaaacgctc gattcaatgg ttcggaaggc aggacatcta
     4561 cggcttcatc taccggagct gggtcaagaa ccgcggcgtg ccgcaggacc agttcgacgg
     4621 ccgtccggtg atcggtatct gcaacacgtg gtcggagctc acgccgtgca acacgcactt
     4681 ccgcgtgctc gccgaacacg tacgcaacgg catccttgaa gccggcggct tcccgctcga
     4741 gtttcccgtg atgtcgctcg gcgagacgtt gatgcggccg accgcgatgc tttaccgcaa
     4801 ccttgcggcg atgagcgttg aagaatcgat ccgcgccaat cccctcgacg gcgtggtgct
     4861 gctcgccggc tgcgacaaga ccacgcccgc gacccttatg ggtgcggcga gcgtcgattt
     4921 gccgacgatc ctcgtctcgg gcggcccgat gctctcgggc aagtaccgcg gaacggaaat
     4981 cggctcgggc accaacacct ggtcgatgtc ggaggacctg cgcgccggca agatcacgct
     5041 ggacgagttc catgaggccg aatcgtgcat gcaccgctcg cacgggcact gcatgacgat
     5101 gggcacggcc tcgaccatgg cctccatgag cgaggcgctc ggcgtcgcca tgccgggcaa
     5161 cgccgcgatt cccgctgtgg acgcgcgccg taacgtcctc gcgcggctcg ccggccggcg
     5221 cgtcgtcgag atggtgaagg agaacctcgt cctctccaag attcttgtgc gcccggcctt
     5281 cgaaaacgcg atccgcgcac tcgcggcgat cggcggctcg acgaacgctg taattcacct
     5341 cgtagcagtg gcgcgccggg tgggcatcga gctttcgctc gacgacttcg acggcctcgg
     5401 ccgcggcgtg cacacactcg tgaatctcat gccgtcgggc aagtacctga tggaggactt
     5461 ctactacgcc ggcggcctgc cggcggttct gcgcgagatt gggccgttgc tgcacaagga
     5521 tgcgctgacc gtgaacggca agacgatgtg ggagaacgtg aaggacgctc cctgctttaa
     5581 ccgcgaggtg atcacgccgt tcgcgcagcc gttcaaggaa aacaccggca tcgcggtgct
     5641 gcgcggcaat ctttgtcccg acggcgccat catcaagccc tccgcggcaa cgccgaagct
     5701 catgcagcac cgcggccgcg cggtggtatt cgaaaacatc gaggacttcc acaagcgcat
     5761 cgatgatccg aatctggatg tggacgagcg ctcggtgatg gtgctcaaga actgcggccc
     5821 gaagggctat cccggcatgg ccgaggtcgg caacatgccg cttccgccca agcttttgaa
     5881 gaaaggcatc accgacatgg tgcgtatctc ggatgcgcgc atgagcggca ccgcgtatgg
     5941 gactgtggtg ctgcacatgt cgcccgaagc ggcggcgggc ggcatgcttg cgctggtcga
     6001 gaacggcgac gagattgagc tcgacgtcgc caagcgaaag ctcgaactct tggtgaacga
     6061 gcgcgagctc acggagcgca aagccgggtg gaaagcgccg cagccaccgc tcgaacgcgg
     6121 ctactggaag ctctactacg accacgtgaa ccaggcgcac gaaggcgcgg atctcgactt
     6181 cctcgtcggc aagagcggcc ccttcgtccc gcgtgataac cactgagttc gcgctctatt
     6241 gcttcgcgca gtcgggaaac gcctacaagg ccgcgctcat gctgcagctc tgcggcgcca
     6301 cgtggcagcc gcgcttcgtc gacttcttcg gcggggagac gcgtacaccc cgctatcgcg
     6361 agatcaacga gatgggcgag gcgccggtgc tcgagcacgg cgcgaagcgg ctctcgcagt
     6421 ccggcgtgat cctgcattac cttgccgacc acttcggcaa gttccagggc cacgacaaac
     6481 tggaagttct gcgctggatc ctgtgggaca accacaagct gacgagctac atcgccacct
     6541 tgcggtggat ggtgacgctc gccaaggtcg gcgaaccgca ggtgcacgaa ttcctccgcg
     6601 ggcgcgtgaa gggcgcgctc ggcatcctcg acgcccacct cggtaagagc gccttcgcgg
     6661 tgggcgacca gccgtcgatc gccgatatct cgatgtgcgg ctacctgtac tggcccgacg
     6721 aattcggcgt atcgtggacc gattacccgc gcatcggggc gtggctcgag cgcatccgca
     6781 agctgcccgg ctgggtgcac ccgtacgagc tgatgcccgg gcatcctcta aagaggtaga
     6841 tatgctccaa gccgtcgaac tgaccatccg cctgcatgcc gaggacgatg tcgtcatcgc
     6901 gcgcgtggat ctccccacag ggacgctcgt cagcaaggag aacgtgcgcg ccgcggtcac
     6961 cataccggcg ggccacaagc tcgccgtgcg cgacattgcg caaggccggc cggtgcggcg
     7021 ctataaccag atcatcggct tcgccacgcg cgacatcaaa gcgggcgagc acgttcacgt
     7081 ccacaacctc ggcatgggcg acttccagcg tgactacgct ttttcttcgc tcaagataga
     7141 aaccgcgtac atcacgcccg ccgccacctt cgagggcatc gtgcgcgccg acggccgcgt
     7201 ggccacgcgc aactacatcg gcatcctgac gagcgtgaac tgctcggcga ccgcggctcg
     7261 catgatcgcc aggcattttg agaacaatct caagcaatac cccaacgtgg acggtgttgt
     7321 ggcgctcact cacaagagcg gctgcggcat ggcatccgag ggggaaggca tggacgtgct
     7381 ccgccgcact atggccggct acgcgcggca tccgaatttt ttcgccacgc aggtgatcgg
     7441 cctgggctgc gaggcgaatc agatcaacaa cctcttatcc acccacaacc tcaaacgcca
     7501 cgaccgcatg cacgcctaca cgattcagga gaagggcggc accatgaagg cggtgcgcga
     7561 aggcatcgca cgcatcgaag cgatcctgcc cgaggccaac aaggtgaagc gcgagaccgt
     7621 cccggcgagc cacctcatcc tgggcctgca atgcggcggt tccgacggct attcgggaat
     7681 cagcgccaac ccggcgctcg gcgccgcggt ggatcttctc gtgcgtcatg gcggcacggc
     7741 aatcctctcg gagacgccgg agaactacgg cgccgagcat ctgctgaccc gcagggcggt
     7801 ttctcaacag gttggagaaa aacttctggc gcggatgcgc tggtgggagg agtacacccg
     7861 gcgcaataac atggaaatga acaacaaccc gtcgcccggc aacaaggcgg gcggcctcac
     7921 cactattctt gagaaatcgc tcggcgccgt cgcgaagggc ggcactacaa acctcgtcga
     7981 tgtctatgag tacgccgagc cggtgagcgc caagggcttc gtctacatgg acacgcccgg
     8041 tttcgacccg gtgtcggcga ccggccaggt cgccggcggc gccaccatga tctgcttcac
     8101 caccggccgc ggctcggcct acggctgcaa gcccgcgccg tcgctcaagc tcgcgaccaa
     8161 cacaccgctc ttcgtgcacc aggaagaaga catggatttc aactgcggca cgatcatcga
     8221 tggcaccgaa tccatcgagc aggcgggcgc gcgcttcttc cagctcatcc tcgacaccgc
     8281 ctccggcaag agatcgaaga gcgaggaatt cggctacggc gacgacgagt tcgcgccctg
     8341 gatcatcggc gccacgatgt aactagttcc tgctccattt gatagagcag ccgaccgagg
     8401 ggatctgctt ctccggtccc ttgccggtct cggcgatgcg cttcatggcc gcgaacatct
     8461 cgtcgtagcg gccgtggtac tcgaggttta ggcccgcgtc gaagccgaag aattccggag
     8521 tgcacaccgt gccataggcg cgcgccactt cctgagtgtg gtcgatgacg tacggaaacc
     8581 cgaagccttt ctcgcgcgcc acccgcttca tgttctcgaa cgaatcgtcc gggtagtcgg
     8641 tagggtcatt cgacatgacc gcaatcgagc cgatgccgag cggcgcgagc cccttgcagt
     8701 cgcgcacgat gccgtcgatc acgcgcttca cataagggca gtgattgcag atgaacatca
     8761 cgagcagtcc cttgtcaccg cgcgcggtga ccggcgagta aaccttgccg tccacgccgc
     8821 tgagctcgaa catcggcgcc ttcatgcaaa aatcctagca tggtgccgcg cttctacgtc
     8881 gatgcacccc tgcgcgcgcg aagcgtgagc acgcttcccg aagacgccgc gcatcatgcg
     8941 gtccacgtgc tgcgcctgcg cgagggcgag ccggtggttc tcttcaacgg ccgcggcggc
     9001 gagttttccg cccgcatcgc ttcgatgaag cggttggaaa tcagcatcga catcctcgag
     9061 catcgggcga tcgagcgcga atcgccgctg cgcgtcacgc tggtacaggg gatatccgca
     9121 agcgacaaga tggacggcac cgtgcgcaag gcggttgagc ttggcgttgc cgaagtgcaa
     9181 ccggtcctgg ccgcacgctc cgtggcgcgg ccgaagggtg agcgcgccga gaaccggcgc
     9241 gcgcactggc agaaggtggt catcgcggcg tgcgagcaat gcggccgtaa ccgaattccg
     9301 caagttcacc ctgtgatggg agcgatggaa tatcgcgcgc cacaagaagc ggcgaagctg
     9361 cttctttctc ccaactcgcg cctgcgcttc gcgcaggcag tgaagacggg cgagaccttc
     9421 gccctggcgg ccggccccga ggccggcttc accgccgacg aggaagcggc cttcgcgtcc
     9481 gccggtttcg tgccggtgtc gctcgggccg cgggtgctgc gcaccgaaac cgccgccgtg
     9541 gcggcgatcg ccgcgctcaa cgcgttgcgg ggcgattccg cgtaatcagc tcggccagcc
     9601 gttcgttgcc gttggaacgc gcgaagtcca ccgcgtcgcg gccccagaag tcggtcgcgc
     9661 cgaggtccac gccctgcccg gcgatgaaga gctccgcggc aatggcgtcg ttgcgcttga
     9721 ccgcgtcgag gaactgcttc gaatcgtggt aggtaagccc catcgaggtg agatctctgc
     9781 gcgcctgcaa cgggtccatg cgcggccgcg cgtccttcac cccgaggttc ttcctcgccg
     9841 ccgcggcgag atcgcggccg atggccgcga tggtagcgcg gtgttgcggg ggcacgtcgg
     9901 caagcatctc cttcaccagg ccggacaccg cctcgttggc gaccgggttg cggccgagga
     9961 gcacctcatc gatggcgcgc aggctctgcg tcggcgtctc cgactcggcg gcgatgcgca
    10021 cgaaaaggtg gatcagcgtg agcgcgtgac cgaaggccgg ccattcctcg ttctgcgcga
    10081 gcgccggcgc ggcgggcgcg atgagcatgg cggcaagcag gatacgaatc gttttcatgg
    10141 gtattgcccc tccgtggtcc ccaaggataa cgcggcgacc cgcttttggt ttcatcgata
    10201 gaatgttgaa aaaattacag tatggagaac gcagggagct tcgtccgctg gttccgccag
    10261 gtcgcgccgt acgtgcacga cttcggcggc cgcaccttcg tgatcgcgtt cggcggcgag
    10321 atggtcgccg agcgggcccg ttttcagagc tttatccacg atgtcaacgt ccttgcctcg
    10381 ctcgagatcc ggcttgtcct ggtgcacggc gcgcggccgc agatcgaagc cgagatgaag
    10441 gcgaaaggaa tgcgctcgcg ctacgcgcag ggcctgcgca tcaccgacga ggccgccctc
    10501 acggtggtga agcacgcggg cggcgtgctg cgcgtggaga tcgaggctct gctctcccag
    10561 gggctacccg attcaccgat ggcgcgcgcg cagatccgcg tcgtatccgg caactacatc
    10621 accgcccgcc cgatcggcgt gcgcaacggc gtggatttcc agttcaccgg cgcggtgcgc
    10681 aaagtcgacg ccgcggcgct tgcggcgcag ctcgacgtcg tggatatcgt ggtcgtgccg
    10741 cacctcggct attcgtccac cggcgaagtg ttcaacctcg cgtgggagga cgtcgcggaa
    10801 agcgtggcgg tggcgctgca cgcggacaag ctgctcatgt acacggacaa gcttcccacc
    10861 agccgcaagg gcgaagtcat ctccgaactc accgccgagg aggccagcgc gcttgcgagc
    10921 gcaaggaaga gcgatctcac gcccaccgcg cggcgcgcga tggaacacct cgcgcgcgcg
    10981 gtcgaaaacg gcgtcgcgcg cggccacctg gtctcgcggc gcgcgcccgg gtcgttgctg
    11041 ctcgagctat tcacgcacgc gggcgtcggc accatgatca ccgccgatgc cgtcgagaaa
    11101 ctgcgcccgg cgcgcgtgga ggatgtgcgc ggcatgctcg cgctcatcga gccgctcgaa
    11161 gccgagggca cgctcgtcaa gcgaagccgc gagctgctcg aagccgagat cggaaatttc
    11221 ctcgtcgtcg agcacgatgc gcgcatcgtc ggctgcgccg cgctctaccc gttccccgac
    11281 gaccggagcg cggagttcgc ctgcctcgcc gtcgacgccg agtaccgcga ggctggctac
    11341 ggcgagcggc tgctcaaagc ctgcgagcag cgggccagag gcctcaagct gcgcaagctc
    11401 ttcgccctta ccacgcacgc cgcgcactgg tttctcgagc aaggcttccg cgcagcgtcg
    11461 gtggacgcat tgcccgagcg tcgcaaggcg ctctacaact ggcggcgcgg ctcgaaggtg
    11521 tttctcaaga gaatataatt tctcgctatg gcgcgaatgg tgaagtgcgt gaagctcggc
    11581 cgcgaggccg agggcctgga gtttcctccg tatcccggcg cgctcggcaa gcgcatctgg
    11641 gagaacgtct cgaaggaagc ctggcagcag tggctcaagc agcagacgat gctcgtgaac
    11701 gagaaccgcc tgaacctcgc cgaccagcgc gcgcgcaagt acctcatgga gcagaccgag
    11761 cgccacttct tcggcgccgg cgcggacgtc gcgtccggct acgttccgcc ggaggacaag
    11821 aagtagcgaa aagccgtcgc aagggccgcg aggccgagcg ccgcagccag gaatacaggc
    11881 gtcatgccgg cgccgtcgac gacgtgcgac gcgagccaca cccccgaagc ctgtcccagg
    11941 aagaggaagg tcgcgaagag cgccaccgcc gagccgcgat cttccggcgc gacctgggtg
    12001 acatgcgttt gcacggttgt atgcagcatg tagaagcccg cgccgaccgc accgatggac
    12061 aaggtggtga gcgtcaggaa tggcgatagc gcaaggccga ggtagccgat aaagagcgcg
    12121 ccgccgccga gcgtcgcgag cccccgttcg ccgagccgcc gtaccgcgcg ccgcgctgcg
    12181 agcgcgtaca gcatgccgcc ggccgcgtac gcggtgacga gcgtcccggc gaggcccggg
    12241 ccgagcagga agcgccgctg gagatagatc gcgacaaaag cgagcgcgcc gaagatgaaa
    12301 agcccttcga catatccggt cgcgatcagc acgcggagcc gcggatggac aaggaggcgc
    12361 ggcatgcgcg cgaggctctg gcggatcgcg ccgcgccctg cgccgcgacg cgcggcgagc
    12421 ggattgcgct tcacttccag aacgagaagc agcgcaacga tgaaaagaag cgcggccatc
    12481 gcgaaaaaga tcgtgcgcca gccgaaatgc tcgccgagcc agccggcggc cgcggtgccg
    12541 aaagcaacgc cggccatctg cccgacgaga aaacgtgcga gaagcggctg gcgcttctcg
    12601 taggaaaccg tgtcgccgat ccaagcgagc gcgagcggaa tgaacgcacc gatggtcgcg
    12661 ccggagacga agcgcgcggc gacgagcgcg ccgagcgatg gggcgagtgc gcacgccgcc
    12721 gaaccgaacg ccgagaccaa tgccgccgcg gcaatcgtgc ggtagcgccc atagcggtcg
    12781 ccgagcggcc cgcaaaccac ctgcaggagg ccgtaggaca gcgcgaacgc cgtgaccgcg
    12841 gccgacgccg cgcctgcggt cacggcgtat tcgtccgcga tgagcggcag caagggatca
    12901 gtcgcgcgca ggctcgctgc gctggcgaaa gccgccagcg acaggaggaa caccgcgcga
    12961 ttcaactaga aaggcgtttc ttggtgagga cgccggaggc ttcggcgagc agcagaacgt
    13021 cggccggtcg gcgcgcgaaa atgccgttcg tcacgacgcc ggcgatctgg ttgagctccc
    13081 cctccacctt cggcggatcg aggagcgtca tgccatggca atccagaacc acgttgccgt
    13141 tatccgtggt gaagccttcg cgcagccgcg ggtggccgcc catcttcacc acttcacgcg
    13201 cgacgtagct gcgcgccatc ggaatcacct cgatcggcac cgggaacttg cccagcaccg
    13261 gcacgagctt cgagccgtcg cagatgcaca cgaacctgcg cgcgacagcg gccgtgatct
    13321 tctcgcgcgt gagtgcgccg ccgccgccct tgatcatatg gagatgctcg gttacctcgt
    13381 ccgcgccgtc gatgtagacc ggaagctcgt tcacgctgtt caggtcgagc acgcgcacgc
    13441 cgattttccg cagcctttcg gcgctcgtct cggaagccgc gaccgcaccg tcgatctggt
    13501 gcttgatgga ggcgagcgcc tcgatgaact tgaacaccgt ggagcccgag cccacgccga
    13561 tgaccgggtc ctgaacgtat ttgagcgcct cgcgcgcaac gcgctccttg agttcgtcct
    13621 gggtcatcgc ttgcgttgcg gcgggagatc ggtgcagacg ccttcgaaga cctccgccgc
    13681 catgccgacc gactcggccg tggtcggatg cggatggatg gtcttgccga tgtcggtcgc
    13741 gtccgcgccc atttcgatgg cgagcgcgat ctcgctgatc aggtcgcccg cgccgaggcc
    13801 aacgatgccg ccgcccacga cgcgatgcgt ctccgcatcg aagatgagct tggtaaagcc
    13861 ttcgtcgcgc gcattggcga tggcgcgccc cgacgcggcc cacgggaact tcgccacgcc
    13921 caccgcgacg tcgcttttct tcgcttcgtc ctcggtgact cccacccagg cgatctccgg
    13981 atcggtgtag gcgactgagg gcacgacgcg cgcgtcgaag tgcgacttct gccccgcggc
    14041 cgcttccgcg gcgacgtgac cttcgtgcac cgccttgtgg gcgagcatcg gattaccggc
    14101 gatgtcgccg atggcgaaga tgttgggcac attcgtgcgt cgctggctgt cgaccgggat
    14161 aaaccccttg gcatcgactt cgacgccggc cttttccgcg ccaatcttcc tgccgttcgg
    14221 cacgcgcccg acggagacga ggatcatgtc gtaggtcttg gcttccgcac cttcgaacga
    14281 aaccttgagc ccgtccttcg tgccctcgac cttggtggtc ttggttttga gcatgatctt
    14341 gtcgaagcgc ggcgcgttga acttctgcca caccgcgacc aggtcgcgat cggcgccaag
    14401 catgaggccg tccatcatct cgacgacgtc gaggcgcgcg ccgagcgtcg agtagaccgt
    14461 gcccatctcg aggccgatga tgccgccgcc gatgatgagc agcttcttcg ggatggagcg
    14521 cagctcgagt gcgccggtcg agtcgacgat gcgcgggtca tccggcagga acggaagctt
    14581 cgccgcctgc gagccggcgg cgatgatcgc gctttggaac cggatggtct tgccgcccac
    14641 gtccatgcgg ttgcggtcga ggaacgtgcc ggcgccctgc accacctgca cctttcgcat
    14701 cttcgccatg ccggcaagcc cgccggtcag cttgccgacc accttgtcct tgaacccgcg
    14761 cagcttggcg agatcgacct tcggctcgcc gaatgtgatg ccgtgcgagg cgagcgcttt
    14821 cgcgccgtcc acgatggccg ccgtgtgcag cagcgctttc gacggaatgc agccgacgtt
    14881 gaggcatacg ccgccgagcg tggcatagcg ctcgaccagc acggtcttca tcccaaggtc
    14941 ggccgcgcga aacgcggccg aataaccacc gggtccggcg ccgaggacga gcacatcgca
    15001 ctcgaggtcc gctccagccg aaggtgaggg tgatgaactc tgcggtgctt caggcgcctt
    15061 gggcggggcc ctcattgccg gcgcctctgc gcgtgtcgct tccgcgtcaa gtatcagaat
    15121 cggcgatccc ttcgaaacct tgtcgccaac cttgaccacc agcttggcaa ccttcccggc
    15181 cgtgggggac ggaatttcca tggtcgcctt gtccgactcg agcgtgatga gtgactgctc
    15241 cttgtcgatc gcgtcgccgg gcttaacaag cacctcgatg acttcgacct ccttgaagtc
    15301 gccgatgtcg gggactttga cttcagtacc tggcattgac tacagaatga gcttccgaat
    15361 gtctgaaagc accgagacca ggaacgcgga gaagcgcgcc gcggtggcgc cgtcgatgac
    15421 tcggtggtcg taggaaagcg aaagcggaag catgaggcgc ggcgcgaatg tctttccgtc
    15481 ccataccggg cgcaaggcgc tgcgcgagac gccgaggatg gcgacttcgg gcgcgttgat
    15541 gatcggcgtg aacgcggtgc cgccaatgcc gcccaggctc gagatcgaaa aggtgccgcc
    15601 ctgcatatcg gccggcgcga gcttgccgtc gcgcgctttc ttcgaaatgt cgccgagctc
    15661 ctgtgcgatg tcgaacacgc ccttgcggtc ggcgtcgcgc acgacaggca ccacgagccc
    15721 gccgggcgta tccacggcta cgccgacgtt gtagtacttc ttgacgatca ggttctcgcc
    15781 cgacttgtcg agcgacgagt tgaactgcgg gtactggcgc agcgccgtga cgcaggcctt
    15841 gatcatgaag gcgagcatcg tcagcttgaa gccttttttc tccgtctcga cggtctgcgc
    15901 cttgcggaag gcctcgaggt ccgtgatgtc cgcttcgtcg aactgcgtga cgtgcggaat
    15961 cgaaatccag ttgcgatgca ggtaaggacc cgagagcttc tggatgcgcg aaagcgcctt
    16021 ggtctcgacc gggccgaact tggcgaagtc gacttcgggc cacgctggaa gattgaacgg
    16081 caacgcccca ccgccctgcg gcgccaccgg cgccttgcct tgcacgatgc ccttcacaaa
    16141 ggcctgcaca tcggtgtgca ggatgcggcc cttcggcccg ctgccctgca cgcgcgaaag
    16201 atcgacgccg agctcgcgag cgaacttgcg caccgaaggg ctcgcgtgcg gcttggcgcc
    16261 cgaagacggc gcctcctcgc gcggcgcagc cgcgggcgca gcggccaccg gcttcggcgc
    16321 ctgctgcggc ttggccactg gaggagcggt gggcttcgcc gttgccgcgt ctttcgaatc
    16381 gagcacgaga atcggcgctc ccgtcgacac ccggtcgccg accttgatct tcagttcctt
    16441 taccacgccc gcggcgggcg acgggatctc catcgtcgcc ttgtcggatt caagcgtgat
    16501 gagggattgc tccttcgtca ccgcgtcgcc cggcttgacc aggacctcga tcacctccac
    16561 gtccttgaag tcgccaatat caggcacggg aaccgtcatc gcactgcccg cctgttgcgg
    16621 ctgggatttc ggcgcttcgg ccttggcaac tggaggtttc agcttttcct cgggagcggt
    16681 ttcgccgtta cctttttcgt ccaagacgag aatcggcgag cctttcgaaa ccttgtcgcc
    16741 caccttgatc ttgagttcct tcaccacacc cgagccgggc gacggaatct ccatcgtcgc
    16801 cttgtcggac tcgagcgtga tgagcgactg ctctttcgac acggcgtcgc ccggcttcac
    16861 cagtacttcg atgacttcaa cttccttgaa gtcgccgatg tccgggacga caacctggtt
    16921 catacggtcg tggggtccga tttctccgga tcgattccgt acttcttgat cgcctcggcc
    16981 acggctttgg ccttgccctc gccctgatcg gcaaacgcct tgagcgcggc gattaccacg
    17041 aaaaagcggt tcacttcgaa gaagtggcgt agcttcgccc gagagtcgga acggccgaag
    17101 ccatcggtgc cgagtacgcg gtagacccgg tcgctggaaa tgaagggccg gatctggtct
    17161 gcgaaggttt tcatatagtc gctcgccgcg atcacgggtc cggccgggcg cttctccagc
    17221 agctgcgtca catagggtgc tttgggcttc gcttccggat gcagcatgtt ccagcggtcg
    17281 acggcgagac catcgcgccg cagctcagtg aagctcgttg cgctccacac gtcggcggcg
    17341 ataccccaat ccttctcgag cagctcggcg gcggcttcaa cctcgcgcag gatggtgccg
    17401 gagcctagga gctggacgcg ttgcttgtgc ttcgcctttg attcgcgcaa gagatacatc
    17461 cccttcagga tcccctcttc cacgccctgc ggcatcgccg gatgttcgta gttctcgttc
    17521 atcaggctga ggtaataata aatgtcctcc tggttggtca ccatgcgccg caggccgtct
    17581 tgaatgatca ctgcgacctc gtagccgtaa gtcgggtcgt acgagaggca attcgggatc
    17641 acggacgaca agatgtgcga gtgaccgtcc tcgtgctgca ggccttcgcc gttcagcgtg
    17701 gtgcggcccg cggtggcgcc aagcaggaag ccgcgcgcgc gctgatcagc ggccgcccat
    17761 gcgaggtcgc cgatgcgctg aaagccgaac atcgaataga aaatgtagaa cggcaccatg
    17821 acgcgattcg aatggctgta cgaggtcgcc gccgcgatcc acgacgagaa ggcgcccgcc
    17881 tcgttaatgc cttcctcgag gatctgcccc gccttgtcct cgcggtagta catcacctgg
    17941 tctttgtcga ccggctcgta cttctggcct tcgggcgcga agatgccgag ctggcggaac
    18001 atgccttcca tgccgaaggt gcgtgcctcg tccggcacga tcggcacgat gtgtctgccg
    18061 agctccttgt cgcgcaccag cgcagtgagc acacgcacga aagccatggt ggtcgagatc
    18121 tcccggcctt caccggaacc cttgagcagc gcatcgaagg ccgcgagctc gggtaccgcc
    18181 ggaacctttt ctgccttctc acggcgctgc ggcacgaagc cgccgagcgc tttcctacgc
    18241 tcgttcaggt atttcatctc gggcgagtca gcgggcggaa tatagaacgg cagctcctcg
    18301 accttgtcgt ccgggatcgg gatggcgaac tgatcgcgca tttccttgat cgtagaaatg
    18361 tcgatcttct tcagctggtg cgtcggattc ttcgcttggc cctgcttgcc aagtccgtag
    18421 cctttgatgg tctttgcaag aatgacggtg ggctggcctt tgtgcttcac ggccgccgcg
    18481 tacgcggcgt acaccttgtg cggatcgtgc ccaccgcggt tcaggcgcca gatgtcctcg
    18541 tcgctcatgc gtgcgacgag ctccagcgct ttgggatgct tgccgaagaa atgcttgcgc
    18601 acgaaggcgc cgtcgttcgc cttgtagttc tggtattcgc cgtcgaccgt ctcctccatg
    18661 atgcgcagga gaatgccctc cttgtcacgc gcgatcagcg gatcccaata cgaaccccag
    18721 atcaccttga tcacgttcca gccggcgccg cggaactcgc cctcgagctc ctggatgatc
    18781 ttgccgttgc cgcgcaccgg gccgtcgagg cgctgcaggt tgcagttcac gacgaagatc
    18841 agattgtcga gcttctcgcg cgccgccagg ccgatggcgc ccatcgactc gggctcgtcc
    18901 atctcgccgt cgccgcagaa cacccagacc ttgcggttcg cggtctgcgc caggccgcgc
    18961 gcgtgcaagt acttcaggaa ccgcgccatg tagatgccct ggatcggtcc gaggcccatc
    19021 gacaccgtgg ggaactgcca gtagtcgggc atcagccacg gatgcggata tgaggagaca
    19081 cctttgccgt cgacttcgcg ccggaaactg agaagccggt ccgcggagag gcgcccttcc
    19141 atgagcgagc gcgcgtaaac gcccggcgag gagtgcccct ggaagtagac caagtcgccg
    19201 ccgtgtcctt cgtgcggcgc attccagaag tgctgctggc ccgtgccgaa gagcgtgccg
    19261 accgaagcga agctcgcgat gtggccgccg agctcgtcgt cgctcttgtt cgccttggcc
    19321 accatgacca tcgcgttcca ccggcaaatc gagcgaatgc gctcttcgag cgcgatgttg
    19381 cccggcgagc gctcctccat gtgcggagga atggtgttga tgtaagcggt attcggcgaa
    19441 tacggaatgt gtgcgcccga gcgtcgcgcc ttgtcgatca accgctccag aagatagtgc
    19501 gcgcgctgtg ggccctcgcg ctcgaggacc gcttcgagcg cgtcgagcca ttcctgcgtt
    19561 tctagcgcgt ccggatcgtt tgctgcggga aactgcggaa cggctgacat gatcgtggca
    19621 tccgttgagc tgcggggggc gcggcaagct taaccgagaa ctcggcccca atgctagact
    19681 gcgcgccccc gtggcagccc agatcctcga cggaaaatcg ctggccgcgt cgatccgcgc
    19741 ggcgctcaaa gagcgggtgg ccgcgcttgt gcagcgcgga gtgaagccgg ggctcgccgt
    19801 cgtgcttgca ggagacgatc cggcgtcgcg cgtttatgtg cggaacaagc gcctggccgc
    19861 gaccgagacc ggcgtgcatt ccgagctgat cgagcttccg gggacaatcg gacagcggga
    19921 gctgcgagag cgcatcgagg gcctgaacgc caaccgcgca atccacggca tcctcgtgca
    19981 gctgccgcta ccgaagccaa tcgacgcggc cgcggtactc gaggccatcg cgccggcgaa
    20041 ggatgttgat ggttttcacg aaatcaacat gggcgcgctc tttgccgggc ggcccgcggt
    20101 cgtgccttgc acacccgccg gcgtcatgcg cctcatggag caggcgcgca ttccgcttgc
    20161 cggccgccac gccgtcgtca tcgggcgctc gaacatcgtc ggcaagcccg tggcgcagct
    20221 cctcttgcaa aaggacgcaa cggtcactat ctgccattcg aagtcaagcg atctttcgga
    20281 actcacacgg cgcgcagacg tcctcatcgc ggccgtcggc agaccgaaac tcgtgcgccg
    20341 ggacatggtc aaacccggag cctgcgtcat cgatgtcggt gtcaatcgcc tcgaagacgg
    20401 gaaactggcc ggggacgtcg atttccaagc ggtgaaagaa gtcgcgggct ggattacgcc
    20461 cgtgccgggc ggcgtcggcc ccatgaccat cgcaatgctg ctggagaact gcatccgtgc
    20521 tgcctcacga gaacttgagc caaaacccgc tggttgattt ttccgggctg ccgcgcttcg
    20581 cggacatccg gcccgagcac gtcactcccg ccatggagct gcttctctcc gacgggcgct
    20641 cgacggtgaa tgcggtcgtg aatgcgcgcc caacctggca cgatttcatc gagccgcttg
    20701 aagacgcgaa cgagcgcatc ggccgcgcgt ggggccaggt cgcgcatctg cacgcggtaa
    20761 tggacagccc ggaattgcgc gaggcctaca actcgaatct accaaaggtc accaagtact
    20821 ggaccgagct cggccagaat caagagctct tcgaaaaata caaagcgctg cgcgccgcgc
    20881 cggtattcgc tgcgctgtcc gcggcaagga agcgcatcgt ggagaacgcg atccgcgact
    20941 tccgcctgag cggcgccgag ctcgcgccgg aaaagaagaa acgctacgcc gaaattcagg
    21001 aagagcttgc gcggctctcg gccaagttct ccgagaacgt gctcgatgcg accaacgcct
    21061 tcggcatctg catcgcgaaa gaggaagccg ccggcattcc cgacgacgtc ctccaggccg
    21121 cgcgcgaagc tgcgcagaaa gatggaaaac aaggcttcaa gttcactctt cacatgccgt
    21181 cttacctgcc ggtgatgcag tacgccgaca atcgcgcgct gcgtgaaaag atgtactacg
    21241 cgagcagcac gcgcgcctcc gaattcggta agccggagtg ggacaacgcc ccgctcatcg
    21301 cgcgcatcgt accgctgcgc cgcgagatgg cgcagctcct cggctacgcg aatttcgccg
    21361 agctctcgct cgtgcccaag atggctgatt cgccgggcca ggtgctcggc tttctcgacg
    21421 acctcgcgcg ccgcgctcgg gcctttgccg aaaaggacgt cggcgagctg cgtgactttg
    21481 cgaaagcgga gctgggtatt gccgagctcc aggcatggga tatcgcgtac gcctcggaga
    21541 agctgcggct caagcgctac tcgttctccg atcaggaggt gaaacagtac ttccccgagg
    21601 aggtcgcgat ccgcgggctt tttcgggtcg cggagactct gtacggcgtg aagttcgttc
    21661 cagcgcaagc gccgacctgg catccggacg tccgcttctt cgaggtgcgc gacgcgagcg
    21721 gcaaggcgat cgggcagttc tacatggatc tgtacgcgcg tgaaacaaag cgtggcggcg
    21781 cctggatgga cgacgcgatc acgcgccgga agaagcgcga ccatgtccag aacccggtcg
    21841 cgtacctcaa ctgcaatttt tcgcggcccg tcggcgacaa gccggcactc ttcacgcacg
    21901 atgaagtgct cacgctcttt cacgagttcg ggcacggcct ccatcacctg ctcacccgcg
    21961 tcgacgatct cggcgtgtcc ggcatcaacg gggtggaatg ggacgccgtc gagctgccga
    22021 gccagttcat ggagaacttt tgctgggagt gggacgtgct gcgcgaaatg acgcgccacg
    22081 tcgacaccgg ccagccgcta cccaaagtcc tctacgaccg catgctcgcg gcgaagaatt
    22141 tccagagcgg gctggcgatg ctgcgtcaga tcgagttcgc ggtgttcgac atgcgcctgc
    22201 attacgactt cgatccggcg agcggccgca gcgcgctcga tttgctcaac gaggtccgca
    22261 agcaggtcgc ggtgctcttt cctcccggat acaaccgctt cccgaacagc ttcggccaca
    22321 tcttcggcgg tggctacgcc gccggttact acagctacaa gtgggccgaa gtcctctcgg
    22381 cggacgcgta cagcttgttc gaggaaacgg gtgtactcag cccggacgcc ggcgcgcgct
    22441 tccgcgaaga aatcctcgcc gtcggcggca gccgcccggc agcggaatcg ttccgcgcgt
    22501 tccgcggccg ggagccgagc gtggacgcgc tcctgcgcca caacggcatg accacggaga
    22561 cggccgcgac ctgaagctcg cgacctggaa cgtcaattcg ctgaaggtgc gcctgccgca
    22621 cctgctcgac tggcttgccg ccgcgcagcc ggacgtgatg tgcctgcagg agacgaaaac
    22681 cgaagaccat aacttcccgc gtgtcgagat cgagcgcgcc ggctaccgca gcgtgttctg
    22741 cggccaaaag gcctacaacg gcgtcgcgat cctcgcgcgc gacaagctgg aagacgtcca
    22801 gtacggcatt cccgattttc ccgatgaccc gaagcgtgtc atcgccgcaa ctctgcgtgg
    22861 cacgcgcatc ctctgcctct atgcgccgaa cggcgaagcg cccaggacgg acaagtatgc
    22921 gtacaagctg cgctggtaca ccgcgctcac aaagtggctc gggcgcaagc tggagcagta
    22981 ccctcgcctc gccatcctcg gcgatctcaa catcgccccg gagccgcgcg acgttcacaa
    23041 cccgaagcgc tgggaaggca agatccactt ctccgagccg gaacgcgctg cgttccgcga
    23101 agtcatcgct gccggagtga cggacgcctt ccgccttttc gagcagcccg aaaagcagtt
    23161 cacctggtgg gactaccggc taaaggcctt cgaacgcggc tggggccttc gcatcgacca
    23221 catcctcctc gcccccgcgc tggcagcgca gtgcgacgcc tgctcgatcg acgtcgagcc
    23281 gcgcaagctc gagcgcccgt ccgaccacgc tccggttatt gcggagctag gggctgccta
    23341 gctcagcgtg acgcaaatca cgcgcctggc ctgaataggt ctagggacca ccccggctat
    23401 gtatcggggg agccccgatg cgagctcgga caatacgaac ttctaatacc tcagttctag
    23461 aaccgccgtg ggattcact
//
LOCUS       DASTPD010000210         6256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1332176, whole genome shotgun sequence.
ACCESSION   DASTPD010000210 DASTPD010000000
VERSION     DASTPD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332176"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VFR57_07000"
     CDS             complement(<1..100)
                     /locus_tag="VFR57_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU0259144.1"
                     /translation="MCRSIKTLFNFKPPATEEEIRAASLQFVRKISG"
     gene            complement(110..1096)
                     /locus_tag="VFR57_07005"
     CDS             complement(110..1096)
                     /locus_tag="VFR57_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259145.1"
                     /translation="MKPLSRRAFVGLAAALAAPRWARGQAASRVYRLGWLSAIDSYKE
                     PYGLAFVQRLRELGFVESDNLTIAYRHADGRIEKLPSAVAELARFDLDVFFGAGLEAS
                     LAALKQVGGDKPIVFVAVDFDPIATGHVVNPARPGGRLTGITAVQSVLPGKRLELVKE
                     LLPSARKVAVLGNDQTVGQLTVTAEAAKRLALDLHVIQFKRPPFDYEAAIAEAVKANA
                     DVLFVLGSALFVPARRLIPELALKARLPSVFHHAQWAEVGGLMSYGFNFPQMWRSAAD
                     MVGKILRGTKAGDIPMEQPTTYELAINAATVRKLGIRIPESIRIRVDRVIDH"
     gene            1150..2196
                     /locus_tag="VFR57_07010"
     CDS             1150..2196
                     /locus_tag="VFR57_07010"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU0259146.1"
                     /translation="MLAKLADELPAGGGYLYEPKWDGFRAIVFRGADEVYIQSRDSRP
                     LDRYFPELHDALLERLPKNCVVDGEIVIATAKGLDFDALQLRLHPAESRVAKLAKEIP
                     SSFVAFDVLFWRKDFRAVPQAKRRVALEEVLGKVKPPVYLTPVTRDRAVAVEWLKRFE
                     GAGLDGVIAKPESAPYLPGKRAMIKVKHARTADCAVAGFRWYKGTKDAVGSLLLGLYD
                     GNGRLQHVGVASSFSMAMRRALAKELAPLRKDALQDHPWREWAGAAAESSRMPGGQSR
                     WSAGKDLSWEPLRPQRVCEVKYDHMQGDRFRHAALFLRWRPDKPPRECRYDQLEVTKS
                     YELEKIFSSGSSGS"
     gene            complement(2151..3341)
                     /gene="ligD"
                     /locus_tag="VFR57_07015"
     CDS             complement(2151..3341)
                     /gene="ligD"
                     /locus_tag="VFR57_07015"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU0259147.1"
                     /translation="MGISNPGKILFPKPKHTKLDLVRYYLAVAEGALRAAGGRPNMLV
                     RYPDGIEGEFFYQKRAPKSRPPWIEVVELRFPSGRAAEEVVPRDAAALAWLANLACLE
                     LHPHPVRAADLEHPDELRVDLDPVPGVKWNQVREVGLVVASVLKELKLVGWPKTSGKR
                     GLHVYVRIHPRWTFDEVRRAALALAREVERRAPQLATSKWWKEERHGVFVDYNQNAKD
                     RTVAAAYSVRPTPNATVSAPLSWGEIADCDPADFTLATMVSRFRQLGDRHAGIDEQPG
                     SIEGLLELFETQGLGDAPWPPHYKKQASEPPRVQPSKRKLPVLEIARAKRQEDALAGL
                     ERWKKRHPEAAAHLEPADVLVDAMRGRYSTWTRIRVILQHVPEALRPPQEPLDPDEKI
                     FSSS"
     gene            complement(3641..4177)
                     /gene="rnhB"
                     /locus_tag="VFR57_07020"
                     /pseudo
     CDS             complement(3641..4177)
                     /gene="rnhB"
                     /locus_tag="VFR57_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            complement(4174..5298)
                     /gene="lpxB"
                     /locus_tag="VFR57_07025"
     CDS             complement(4174..5298)
                     /gene="lpxB"
                     /locus_tag="VFR57_07025"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU0259148.1"
                     /translation="MVAGEASGDQLAASLIAALKARRSPTLFAGIGGPRMQAQGFDSH
                     YPLEKLSVRGYAEVVKHYGEIMTIRRRLTRALLAEHPDVYIGVDASEFNLGIERRLKE
                     AGIPAIHYVSPQIWAWRGWRVRRIARSVTRILVMFPFEAPLYEKAGVPVTYVGHPLAD
                     VIPIAPNKLEARAALRLPAGKLIVALLPGSRRSELQYMAKSFVLAAHRFRQEVADVHF
                     VCPTVTRDTRDMFERAAHGHDHTDLPLTLLFGHSHEALAAADLALVASGTATLEAALF
                     KTPMVITYRQSPITWALMRRMFYLPYVGLPNVLAGEKLVPELLQHEANPAALAGALLK
                     LLRDTDAQRRQIERFHEFHHLLRQRAAEKAADAILEVLKK"
     gene            complement(5322..6092)
                     /gene="lpxA"
                     /locus_tag="VFR57_07030"
     CDS             complement(5322..6092)
                     /gene="lpxA"
                     /locus_tag="VFR57_07030"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU0259149.1"
                     /translation="MILSTAVVHRGAALAADVEVGPYSVIGEHVEVGEGSWIGAHVML
                     DGHTRIGRRNRIWHFCSIGAPPQDKKYAGEPTRVEIGDDNTIREYVTINRGTALDAGV
                     TRVGNENWVMAYVHFAHDCQIGDRAIFANSCQLAGHVTVGDWAIFGATTLVHQFVHIG
                     AHAFTGMGTYLPQDLPPFVTAAGNMAQPFGINKEGLKRRGFSPETIAGLKRAYKTLYR
                     RGLGLEEALEELKGQAATCPEVQQILEFISRSKRGIIR"
     gene            complement(6089..>6256)
                     /locus_tag="VFR57_07035"
     CDS             complement(6089..>6256)
                     /locus_tag="VFR57_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HEU0259150.1"
                     /translation="ARFKKPVVPGDQLELEVIMERALRGIGKFTCEARVDGVTVAEAT
                     ILCSVRPVDQR"
BASE COUNT         1020 a         2005 c         2139 g         1092 t
ORIGIN      
        1 ggccggagat cttgcgaacg aactgaagcg atgccgctcg gatttcttcc tcggtggcag
       61 gcggcttgaa attgaacagc gtcttaatgc tgcggcacat gcgctactcc taatggtcaa
      121 tgacgcggtc gacacgtatc cggatcgact cgggaatgcg gatgccgagc tttcggacgg
      181 tcgctgcgtt gatcgcgagc tcgtaggtgg tgggctgctc catggggata tcccccgcct
      241 tggtgccgcg caggatcttg ccgaccatgt cggcggcgct gcgccacatt tgggggaaat
      301 tgaagccata ggacatcagc ccgccgacct cggcccactg ggcgtggtga aagacgctcg
      361 gcaggcgagc tttcagggcg agctcgggaa tcaggcgccg cgccggcacg aagagcgctg
      421 atccgaggac gaagagcaca tcggcgttgg ccttcaccgc ctctgcaatg gcagcttcgt
      481 aatcgaatgg cgggcgcttg aactgaataa cgtgcaaatc cagcgccagc cgtttggcgg
      541 cttccgccgt tacggtgagc tggccgacgg tctgatcgtt gcccagcact gccacctttc
      601 gcgccgacgg caagagctcc ttcactagct caaggcgctt gccgggcagc accgactgca
      661 ccgcggtgat gcccgtcaac cggccgccgg gccgcgcggg gttgacgacg tggccggtgg
      721 cgatcgggtc gaagtccacc gcgacgaaga cgatgggctt atccccgccg acctgcttta
      781 atgcggcgag cgacgcttcc agcccggcgc caaagaatac gtcgaggtca aagcgggcaa
      841 gctcggctac ggccgacggc agtttctcga tcctgccatc ggcgtgccgg taggcgatcg
      901 tcaggttgtc gctttccaca aagccgagct cgcgcaggcg ctgtacgaac gcgaggccgt
      961 acggctcttt atagctgtcg atggcgctga gccagccgag ccgatagacg cgcgaggcgg
     1021 cctggccgcg agcccatcgg ggcgccgcca gcgcggcggc gaggccgacg aaggcgcggc
     1081 gcgagagcgg tttcatggca cgtcgctggc tatgatggat ggcagatgat atcccctccg
     1141 atagagccga tgttagcgaa gctcgcggac gagctgccgg cgggtggcgg ctatctgtac
     1201 gagccgaagt gggatggatt ccgggcgatc gtctttcgtg gcgccgatga ggtctacatc
     1261 cagagccgcg actcgcggcc gctcgaccga tattttccgg agctgcacga cgcgctgctc
     1321 gagcgcttgc cgaaaaactg cgtggtcgac ggcgagatcg taatcgcgac ggccaagggc
     1381 ttggatttcg acgcgcttca gctcaggctg catccggccg agtcgcgggt cgcgaaactg
     1441 gcgaaagaga taccctcatc tttcgttgct ttcgatgtgc ttttctggcg caaagacttc
     1501 cgtgcggtgc cgcaggcgaa gcgtcgcgtt gctttggaag aagtattggg caaggtgaag
     1561 ccacccgtgt atctcacacc ggtgacgcgc gatcgcgccg tcgcagtgga gtggctcaaa
     1621 cgcttcgagg gtgcaggcct cgacggggtg atcgccaagc cggaatccgc cccctatttg
     1681 ccgggcaagc gcgccatgat caaggtgaag catgcgcgca ccgcggactg cgcggtcgcg
     1741 ggctttcgct ggtacaaggg cacgaaggat gcggtgggct cgctgctgct cggcctgtac
     1801 gatggcaacg ggcggctgca gcacgtcggc gtggcgtcgt cattctctat ggcgatgcgg
     1861 cgcgcgctcg cgaaagaact cgcaccgctg cgcaaggatg cgttgcagga ccatccttgg
     1921 cgcgaatggg ccggcgccgc agccgaatcg agtcgaatgc ccggcggaca gagccgctgg
     1981 agcgccggga aggacctgtc gtgggagccg ttgcgcccgc aacgcgtgtg cgaggtgaag
     2041 tacgaccaca tgcagggtga ccgcttccgc cacgctgcgc tcttcttgcg ctggcgcccg
     2101 gacaagccgc cgcgcgagtg ccgctacgac cagctcgagg ttacgaaatc ctatgagctg
     2161 gagaagatct tttcatccgg gtcgagcggt tcttgaggcg gccgcagggc ttcggggacg
     2221 tgctgcaaga tcacccggat gcgggtccaa gtgctgtagc ggccgcgcat ggcgtcgacg
     2281 agcacatctg caggctcgag gtgcgccgca gcctcggggt ggcgcttctt ccagcgctcc
     2341 aggcccgcca atgcgtcttc ctgacgtttg gctcgggcaa tctccagcac cggcagcttg
     2401 cgcttggagg gctgaacgcg gggcggctcg gaggcttgct tcttgtaatg cggtggccag
     2461 ggagcatcgc cgagcccttg cgtttcgaat agctcaagaa gcccttcgat ggaccccggc
     2521 tgctcgtcga tcccggcatg ccggtcgccg agctgccgga agcgactcac catggtggcc
     2581 aaagtgaagt cggccgggtc gcagtcggcg atttcgcccc aggaaagcgg cgcggaaacc
     2641 gtggcgttcg gcgtcgggcg caccgagtac gccgcagcca cggtgcggtc cttggcgttc
     2701 tggttgtagt cgacgaacac gccgtggcgc tcctctttcc accacttgct ggtggcgagc
     2761 tgcggcgcgc ggcgctcgac ttcacgcgcg agggcaagcg ccgcgcggcg tacttcgtcg
     2821 aatgtccagc gcggatggat gcgcacatag acgtgcagcc cgcgcttgcc ggaagtcttg
     2881 ggccagccga ccagcttcag ttctttcaag accgacgcga caacgagacc cacttcgcga
     2941 acctggttcc acttcacgcc ggggacggga tcgaggtcca cgcgcaactc gtcgggatgc
     3001 tcgagatcgg cggcgcgcac tgggtgcggg tgcagctcca ggcaggcgag gttcgcgagc
     3061 caggcgagcg ctgcggcgtc ccgggggacc acctcctcgg ccgcgcgccc ggaagggaag
     3121 cgcagctcga ccacttcgat ccaaggcggg cggctcttcg gcgcgcgctt ctggtagaag
     3181 aactcaccct cgatgccgtc cgggtagcgc accagcatgt tcggacggcc gcctgcggcg
     3241 cgcagcgcgc cctcggctac ggcgaggtag tagcgcacaa gatccagctt ggtgtgtttg
     3301 ggcttcggga aaagaatctt cccggggttg gagatcccca catcccgccc ggcgacggcc
     3361 agcagttcct tggctacttt gcccgtagtt gagcgcgcgt gtcgcgcaga agcgcccgga
     3421 cgagggcaac gtgcggggcc gcgtcgcgcg aggcgaacac gtccgcatac tcccgcttga
     3481 ggcgcttcgt gcggtattcg aagtcggcga gctgccgcgc catgctggcg agcttcatcg
     3541 tctcgcaata cagccggtag ctgcggatgc gaagcgattc ggccatcgtt tcccaagtcg
     3601 cgccgaagag gttgccttgg aaaaatacac ccacggcgta gaagctcttg cggtggatct
     3661 cgcagggacc gtggcggccc acggcttcga ggtgctcggc ggtggcgtag cccttgtgct
     3721 ggtcgaagcc gtactgcggg aagcggtcgt gcagcgcgcg catttcggcg tcgcgcgccg
     3781 tcttggcgag gatcgaggcg gccgagatgg gcttgtgcgt ggcgtcgccg tcgacgatgg
     3841 cgcgcgccgc gcaaggcagg tccgggcaca tattgccgtc gacccacgcc tcctcgggtg
     3901 tgattttcaa tccttctacg gcccttctca tggcgaggag cgatgcgcgc aggatgttga
     3961 tgcggtcgat ctcctccacg ctcgcatgcg cgaccgccca cgcgatggcg cgctccttga
     4021 tgcgcccggc gagcacttcg cggcgctccg gtgtgagcgt cttcgagtcg gcgaggccgt
     4081 tcacgcgccg tgtgggatcg aggatcaccg cggctgcgta gaccgggccg gcgatcggcc
     4141 cacggccggc ttcgtccacg ccgcagacca gggtcatttc ttcaacacct cgagaatggc
     4201 gtccgccgct ttctccgccg cccgctgtcg cagcaggtga tggaattcgt ggaagcgctc
     4261 gatctggcgg cgttgcgcgt ccgtgtcccg cagcagcttg agcaacgcgc cggcgagcgc
     4321 cgcggggttc gcctcgtgtt gcaggagctc cggcaccagc ttctctccgg cgaggacgtt
     4381 cggcaggccg acgtacggca ggtagaacat ccggcgcatt agcgcccatg ttatcggaga
     4441 ctgccggtag gtaatcacca tcggcgtctt gaaaagcgcc gcttcgaggg ttgcggttcc
     4501 gctcgccacc agcgcgaggt cggcggcggc gagcgcctcg tgcgaatgac cgaagagcag
     4561 cgtgagcggc aaatcggtgt ggtcgtgccc gtgcgccgcg cgctcgaaca tgtcgcgcgt
     4621 gtcgcgcgtc accgtggggc agacgaagtg cacgtcggcg acttcctgcc ggaagcggtg
     4681 tgcggcgagc acgaacgact tggccatgta ctggagctcg gagcgccggc tgccgggaag
     4741 cagtgccacg atcagcttgc cggccggcag gcgcagcgcc gctcgcgcct cgagcttgtt
     4801 cggggcgata gggatcacgt cggcgagcgg atggccgacg taagtcaccg gcacgcccgc
     4861 cttctcgtac agcggcgcct cgaacgggaa catcaccagg atgcgcgtca ccgagcgggc
     4921 gatgcggcgc acgcgccagc cgcgccacgc ccatatctgc gggctcacgt agtggatagc
     4981 cgggatgccg gcttccttga gcctgcgctc gatgccgagg ttgaattccg acgcatcgac
     5041 gccgatgtag acatccgggt gctccgcgag cagcgctctg gtgaggcgcc gccggatcgt
     5101 catgatctcg ccgtagtgtt tcacgacctc ggcatagccg cgcaccgaga gcttttccaa
     5161 ggggtagtgc gagtcgaaac cctgcgcttg catgcgcggc ccgccgatgc ccgcaaacag
     5221 ggtcgggctg cgccgcgctt tgagtgccgc gatgagcgac gcggcgagtt ggtcgcccga
     5281 cgcctcgccg gccaccatgg cgacgcgcaa tagatcggcc actaccggat gattccccgc
     5341 ttgcttcgag aaatgaactc gaggatctgc tgcacctcag ggcaggtcgc ggcctgcccc
     5401 ttcagctcct cgagcgcttc ctccagcccg aggccccttc ggtaaagcgt cttgtaggcg
     5461 cgcttaaggc cggcgatggt ctcaggcgag aagccgcggc gcttcagccc ctccttgttg
     5521 atgccgaaag gctgcgccat gttgccggcg gcggtgacga acggcggcag gtcctgcggc
     5581 aggtaggtcc ccatcccggt gaacgcgtgg gcgccgatgt ggacgaactg gtgcaccaag
     5641 gtcgtcgcgc cgaagatcgc ccagtcgccc acggtcacgt ggccggcaag ctggcacgag
     5701 tttgcgaaga tggccctgtc accgatctgg caatcgtgcg cgaagtgcac gtaggccatc
     5761 acccagtttt cattgccgac gcgcgtgacc ccggcatcga gcgccgtgcc gcgattgatg
     5821 gtcacgtact cgcgaatcgt gttgtcgtcc ccgatctcca cccgggtcgg ctcgcccgcg
     5881 tatttcttgt cctgcggcgg cgcgccgatc gagcagaagt gccagatccg gttgcggcgt
     5941 ccgatccgcg tatggccgtc gagcatcacg tgcgccccga tccagcttcc ctcgcccacc
     6001 tccacgtgct cgccgatgac ggaatacggt ccgacctcga cgtcagcggc aagcgccgct
     6061 ccgcgatgca ccaccgcggt ggaaagaatc atcgctgatc gaccgggcga accgaacaaa
     6121 ggatcgtcgc ttcggcgacc gtgacgccgt ccacgcgcgc ctcgcaggtg aatttgccaa
     6181 taccgcgcag cgcgcgctcc attatgacct cgagctcgag ctggtcgccc ggcaccacgg
     6241 gcttcttgaa acgcgc
//
LOCUS       DASTPD010000211         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_720435, whole genome shotgun sequence.
ACCESSION   DASTPD010000211 DASTPD010000000
VERSION     DASTPD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720435"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..339
                     /locus_tag="VFR57_07040"
     CDS             46..339
                     /locus_tag="VFR57_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259151.1"
                     /translation="MKNAMWVLWPSFIAGAVGTVLFFALLDPVDLRFVGPLELGRKAG
                     YTLAFFFFWALAASSSWLTCLLQRRADEVNRCPLTPEERPLGCPKREEPNAAR"
     gene            326..1177
                     /locus_tag="VFR57_07045"
     CDS             326..1177
                     /locus_tag="VFR57_07045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0259152.1"
                     /translation="MRLASALFALLPLSVSAQDTASGKVLYETYCGGCHYERVHQRMR
                     SGVRDLSDLRDTVARWSRQTKRTYTLDELENIVQYLNESHYRFGLAPRTHQHRFGDAE
                     KWARVFDDPERDAWQKPHQVIQALALKPDAVVADLGAGTGYFSVRLARMLPRGTVYAA
                     DVEPDMVKYLGERAKREDLSNLRPVTSSGNDARLPAKVDLVLLVDVYHHIDDRERYFR
                     GLAGSLAPGGRVAVIDFKLDSPRGPPKSSRVDPERVKAELARAGYALTTEHAFLPDQY
                     FLEFSRR"
     gene            complement(1174..2508)
                     /locus_tag="VFR57_07050"
     CDS             complement(1174..2508)
                     /locus_tag="VFR57_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU0259153.1"
                     /translation="MKKTLLAAAAAFAPLAAHATDGYFPHGHGMKAKGMGGASMAMTS
                     DALAGADNPAAMAFVGNRLDLGLDWFRPDRSMTREGSAGGAGFFDGSAEGNETQNFFV
                     PEFGYNKMLRPDLSLGISVVAHGGMNTDYPSGQLDLGFCNPLAPAPTGTRTGNMLCGT
                     GNLGVDLAQLVFAPTLAYQFAAGQSLGVAPLIGYQRFKAYGLQAFAAISSDPANLTNK
                     GYDDSWGVGMRVGYYGRLTSTLAVGAAYATKIDMQEFDKYRGLFAEQGDFDIPENYGI
                     GIAFHATPKLMIAADYMRINYNKVKSVGNPSNQPNCTPTPTGGVGPDCLGASGSSIGF
                     GWQNVDVFKIGIEYQWSSTLVLRAGYNKSDNPIEPRDVTINILAPGVIEEHVTLGFTY
                     SWGKSTEITMAYMHAFENSVSGPANNPYFPVGGTETITLSEDSLGIALGWKF"
     gene            complement(2534..3127)
                     /locus_tag="VFR57_07055"
     CDS             complement(2534..3127)
                     /locus_tag="VFR57_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU0259154.1"
                     /translation="MDNTDLDRLIEQRLDALLPKKLAELREKQTPSLCIIATKGTLDW
                     AYPPFILSSTAAALGWNVSVFFTFYGLNLLKKDLSGLKVSPLGNPGMPMKMPFGPEWF
                     KHINWNIPNAVQGIVPGFETLATTLMKKTIHDKGVAGVEELRVACVEGGVNLIGCQMT
                     VDLFGFTPNDFIAEVKEYCGAATFLPMARDADVSLFI"
     gene            3205..3489
                     /locus_tag="VFR57_07060"
     CDS             3205..3489
                     /locus_tag="VFR57_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0259155.1"
                     /translation="MSVRYSTVYIAPMDPVMHNPEAKKAMDARLARIEGQVRAVRRMI
                     EEDRTCENIAQQLSAARRALDRAFYEMVSCMIRHEPEGAGKVADLLARFG"
     gene            3508..3735
                     /locus_tag="VFR57_07065"
     CDS             3508..3735
                     /locus_tag="VFR57_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU0259156.1"
                     /translation="MNFDKELDARGLNCPLPILRAKKALNDMQSGQVLKIVATDPGSV
                     KDFQAFSKQTGNELLSHAAAQKEFTFYLKRK"
     gene            complement(3748..>4197)
                     /locus_tag="VFR57_07070"
     CDS             complement(3748..>4197)
                     /locus_tag="VFR57_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0259157.1"
                     /translation="EIVLYHVEPGQGCLLSGGCLLGHSDYGASGVAETDATLLSIPPA
                     LFQELLVEHEPFRRFVFGMYGERLAQVMQLVEEVAFRRLDARLAQLLVQRGPVVAATQ
                     QKLAEELGSVREIVSRLLRQFEARGWVELGRERITVRDARALAELAR"
BASE COUNT          732 a         1382 c         1349 g          734 t
ORIGIN      
        1 gatcgaggat ccgcgcggca gctggcgcat tctcaaggag gcgtcatgaa aaacgcgatg
       61 tgggttcttt ggccttcttt catcgccgga gcggtaggca cagtgctctt ctttgccctt
      121 ctcgatccgg tagacctgcg cttcgtcgga ccgctcgaac tcgggcgcaa ggcgggctat
      181 accctggcgt tcttcttctt ctgggcgctt gccgccagct cgagctggct cacctgcttg
      241 ctgcaacggc gcgccgacga agtgaaccgc tgtccgctta cgcccgagga gcggccgctc
      301 ggctgcccga agcgcgagga acccaatgcg gctcgctagc gcgctgtttg ccctcttgcc
      361 cttgtccgta tccgctcagg acaccgcaag cggaaaggtg ctctacgaga cgtactgcgg
      421 cggctgccac tacgagcgcg tgcaccagcg catgcgctcc ggcgtgcgcg atctctccga
      481 cctgcgcgac accgtggcgc gctggtcacg tcagaccaag cgcacgtaca cgctggacga
      541 gctcgagaac atcgtccagt acctgaacga gtcgcactat cgcttcgggc ttgcgccgcg
      601 cacccaccag caccgtttcg gcgacgccga gaaatgggcg cgcgtatttg acgatcccga
      661 gcgcgacgcc tggcagaagc cgcaccaggt catccaggct ctcgcgctca agcctgacgc
      721 cgtcgtcgcc gatctgggag ccgggaccgg ctatttctcg gtgcggctcg cccgcatgct
      781 gccgcggggc acggtctatg cggcggacgt ggagcccgac atggtcaagt acctcgggga
      841 gcgcgcgaag cgcgaggacc tgagcaatct caggcccgtc acatcaagtg gcaacgatgc
      901 gcggctgccg gcgaaggtgg atctcgtcct gctcgtggac gtctaccacc acatcgacga
      961 tcgggagcgc tacttccgcg ggcttgccgg ctcgctcgcc cccggcggcc gcgtggcggt
     1021 gatcgacttc aagctcgatt cgccgcgcgg gccaccgaag agctcgcgcg tcgacccaga
     1081 gcgcgtcaag gccgagctag cccgggccgg ctacgcgctg actaccgagc acgcgttcct
     1141 ccccgaccag tacttcctcg agttctcgcg tcgctagaac ttccagccga gcgcgatgcc
     1201 gagcgagtcc tccgagaggg tgatcgtctc ggtgccgcca acggggaagt acgggttgtt
     1261 tgcaggccca gataccgagt tctcgaacgc gtgcatgtag gccatcgtga tctcggtcga
     1321 cttaccccaa ctgtaggtaa agcccagcgt gacgtgctcc tcgatgaccc cgggagcgag
     1381 gatgttgatc gttacgtcac gcggctcgat cggattgtcg ctcttgttgt agcccgcgcg
     1441 cagcacgagg gtgctcgacc attggtactc gataccgatc ttgaacacat cgacgttctg
     1501 ccagccgaac ccgatgctcg agccgctcgc accgaggcag tcagggccga cgccgccggt
     1561 gggcgtgggg gtgcagttcg gctggttgct cgggttgccg accgacttca ccttgttgta
     1621 gttgatgcgc atgtaatcgg cggcgatcat taacttcggc gtggcgtgga acgcgatccc
     1681 gatgccgtag ttctccggga tgtcgaagtc gccctgctcg gcgaagaggc cgcggtactt
     1741 gtcgaactcc tgcatgtcga tcttggtcgc ataggcggcg ccgaccgcga gggtactcgt
     1801 gaggcggccg tagtagccga cgcgcatgcc gacgccccag gagtcgtcgt agcctttgtt
     1861 cgtgaggttc gccgggtccg aggagatcgc cgcgaaggcc tgcaggccgt aggccttgaa
     1921 gcgctggtag ccgatgagcg gcgccacgcc gagcgactgg ccggccgcaa actggtaagc
     1981 gagggtgggc gcgaacacga gctgagcgag gtccaccccc aggtttccgg tgccgcaaag
     2041 catgtttccg gtgcgcgtac cggtgggcgc gggcgcaagc ggattgcaaa agccgaggtc
     2101 gagctggccg ctcggatagt cggtgttcat gccgccgtgc gccaccaccg atataccgag
     2161 cgagagatcc ggccgcagca tcttgttgta gccgaactcg gggacgaaga agttctgcgt
     2221 ctcgttgcct tccgcggagc cgtcgaaaaa gccggcgccg ccagcgctgc cctcgcgcgt
     2281 catcgagcgg tcgggcctga accagtcgag cccgaggtcg agccggttgc cgacgaacgc
     2341 catcgccgcg gggttgtccg ccccggcgag agcgtccgac gtcatcgcca tcgacgcccc
     2401 gcccattccc ttcgccttca tgccgtggcc gtgcgggaaa tagccgtcgg tcgcgtgcgc
     2461 cgcgagcggc gcgaaagccg ccgcagccgc aagcagtgtc ttcttcacaa cccctcccct
     2521 aagtgacttt cttttatatg aaaaggctta cgtcggcatc ccttgccatc ggcaggaacg
     2581 tggccgcgcc gcagtactcc ttcacctcgg caatgaaatc gttcggcgta aaaccgaaga
     2641 gatcgaccgt catctggcag ccgatcaggt tcacgccacc ttcgacgcac gccacgcgca
     2701 gctcctccac cccggcgacg cccttgtcat ggatggtctt cttcatgagc gtggtggcga
     2761 gcgtctcgaa ccccggcacg atgccctgca ccgcgttcgg gatgttccag ttgatgtgct
     2821 tgaaccactc ggggccgaac ggcatcttca tcggcattcc cggattgccg agcgggctca
     2881 ccttcagacc gctcaggtcc tttttcagca aattgagccc gtagaacgtg aagaacaccg
     2941 aaacgttcca gccgagcgcc gcggcggtgc tcgacaagat gaagggcggg tacgcccagt
     3001 cgagcgttcc cttcgtggcg atgatgcaaa gcgacggggt ctgcttctct ctcagctcgg
     3061 cgagcttctt cggaagaaga gcgtcgagac gctgctcgat caggcgatcg aggtcggtgt
     3121 tgtccatggc gtgcctcctg aaatgtagta ctccacggga gtatggggta tcgaattcct
     3181 gtatggaagg atgcagattc gctgatgtcg gtccgatact ccacggtgta tattgcgccc
     3241 atggaccccg taatgcacaa cccggaagca aagaaggcga tggacgcgcg gcttgcgcgc
     3301 atcgaaggcc aagtgcgcgc ggtgcggcgc atgatcgagg aggacaggac ctgcgagaac
     3361 atcgcgcagc agctttcggc cgcccgtcgg gcgctcgacc gggcgttcta cgagatggtg
     3421 tcgtgcatga tcaggcacga gcccgagggc gccggcaagg tcgccgacct gctggcacgc
     3481 ttcggctaac cgagagagag cccgggcatg aacttcgaca aagaactcga cgcgaggggc
     3541 ttgaactgcc ccctccccat cttgcgcgcc aagaaggcgc tgaacgacat gcagtccggg
     3601 caggtgctga agatcgtggc gaccgatccc ggctcggtga aggacttcca ggccttctcc
     3661 aagcagaccg gcaacgagct tctctcgcac gccgcggcgc agaaagaatt cactttctac
     3721 ctgaagcgca agtagaagcg gcggcgatta ccgggcgagc tcggcgagcg cgcgcgcgtc
     3781 gcgcaccgtg atgcgctcgc ggccgagctc cacccagccg cgcgcctcga actgccgcag
     3841 gagccggctt acgatctcgc gcacgctgcc cagctcttcc gcgagcttct gctgcgtcgc
     3901 cgccaccact ggcccgcgct gaacgagcag ttgcgcgagc cgcgcgtcca ggcggcggaa
     3961 cgccacctcc tcgacaagct gcatcacctg cgccaggcgc tccccgtaca tgccgaacac
     4021 gaagcgccgg aacggctcat gctcgacgag cagctcctgg aacagcgccg gcgggatact
     4081 aaggagcgtc gcgtcggtct cggccacgcc cgaggcgccg tagtcggagt gcccgagcag
     4141 gcagcccccg gagagcaggc acccctgccc cggctccacg tggtagagca cgatctc
//
LOCUS       DASTPD010000212         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1129871, whole genome shotgun sequence.
ACCESSION   DASTPD010000212 DASTPD010000000
VERSION     DASTPD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129871"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /gene="infB"
                     /locus_tag="VFR57_07075"
     CDS             <1..1571
                     /gene="infB"
                     /locus_tag="VFR57_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU0259158.1"
                     /translation="KLDDPEAYLAESGEHAAAELKPRPPVVTVMGHVDHGKTSLLDFI
                     RRTKVAAGEAGGITQHIGAYHVETPRGVITFLDTPGHEAFTAMRARGAKATDIVVLVV
                     AADDGVMPQTVEAINHAKAANVPLVVAMNKIDKHEANPDKVKQELLAHGVVPEEYGGD
                     SPVVPVSAKTGEGVDSLLEQVLLQAEVLELRAATEAPAKGVVIEARLDKGRGPVATLL
                     VQSGTLRRGDIVLTGAVYGRVRAMLDENGKPVQSAGPSIPVEIQGLSEVPTAGEEAMV
                     IADERKAREIALFRQGKFRDVKLAKQQAAKLDNMFEQMGEGEKKVLTLVIKADVQGSQ
                     EALVHALTRLATDEVKVTVVHAGVGGITESDVNLAMASRAVIIGFNTRADATARKLIE
                     SAGIQVRYYNIIYDAVEEIKAALSGMLAPERKESVLGLVEVRQVFRISKIGTVAGCYV
                     QEGLVKRSAQVRVLRDNVVIHTGEIESLKRFKDDVREVKAGFECGISLKNFSDIKEKD
                     QFEVFEVLEVARTL"
     gene            1568..1918
                     /gene="rbfA"
                     /locus_tag="VFR57_07080"
     CDS             1568..1918
                     /gene="rbfA"
                     /locus_tag="VFR57_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU0259159.1"
                     /translation="MKRGQGRPQKLGDLIQRELSELLQRELRDPRVGMITITGVDVSP
                     DFSHAKVFFTVLEKQRLEDAREGLRRAAGFLRSRLARRIKLYTTPELRFEYDESVERG
                     DRLSRLINSVTGKA"
     gene            1915..2829
                     /gene="truB"
                     /locus_tag="VFR57_07085"
     CDS             1915..2829
                     /gene="truB"
                     /locus_tag="VFR57_07085"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU0259160.1"
                     /translation="MIDGALLLDKPVGVSSNATLQQAKKLVGATKAGHAGTLDPLASG
                     LLVALFGEATKFAGPLLDADKEYLATLELGSRTRTGDAEGEIMERKPVNITAAALAAV
                     LERFRGEIEQVPPMHSALKRKGTPLYRLARRGEEVTRAARRVRISSLDTVSLEVPLLR
                     MRVGCSKGTYIRVLAEDIGAALGCGAHLAALRRTGSGRFRVENALTLEALEALPIHER
                     GRALLGLPALVHDLPRAELGAAEEARLRQGQALKISGLERGLCAVVRPDGRVIGLGTA
                     DGDGGLKPLRLTQTAEKHLKSAAHMKRE"
     gene            2834..3103
                     /gene="rpsO"
                     /locus_tag="VFR57_07090"
     CDS             2834..3103
                     /gene="rpsO"
                     /locus_tag="VFR57_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0259161.1"
                     /translation="MALQVVQKERVVKQYQRSGKDTGSPEVQIALLTERINSLTEHFK
                     THVKDFHSRRGLLKLVSQRRKLLDYLKRKDADKYRGLIERLSLRK"
BASE COUNT          586 a          976 c         1061 g          480 t
ORIGIN      
        1 ccaagctgga cgacccggag gcttatctcg ctgaaagcgg ggagcatgca gcggcggagc
       61 tcaagccgcg cccgccggtc gttacggtca tgggtcacgt cgaccacggg aagacctcgc
      121 tcctcgattt catccgccgc accaaggtgg ccgcaggcga agccggcggc atcacgcaac
      181 acatcggcgc ctaccacgtc gagacgccgc gcggcgtaat cacgtttctc gacacccctg
      241 gccacgaagc ctttaccgcg atgcgcgcac gcggcgccaa ggcgaccgac atcgtcgtgc
      301 tggtggtcgc cgcggacgac ggcgtgatgc cacagaccgt cgaggcgatc aaccacgcca
      361 aggcggcgaa cgtgcccctc gtcgtggcga tgaacaagat cgacaagcac gaggcgaacc
      421 ccgacaaggt gaagcaggag cttctcgccc acggggtggt gcccgaggaa tacggcggcg
      481 actccccggt agtcccggtc tccgctaaaa ccggcgaggg ggtcgatagc ctgctcgagc
      541 aggtgctgct gcaggccgaa gtgctcgagc tgcgggcggc aaccgaggcg ccggccaagg
      601 gcgtggtgat cgaggcgcgc ctcgacaagg gccgcggccc ggtcgccacg ctgctcgtgc
      661 agtcgggaac cctgcggcgc ggcgacatcg tgctgaccgg cgcagtctac ggccgtgtgc
      721 gcgccatgct cgacgagaac ggcaagcccg tgcagtcggc cgggccgtcg attccggtcg
      781 agatccaggg gctttccgag gtgccgacgg cgggcgagga agcgatggtc atcgccgacg
      841 agcgcaaagc gcgggagatc gccctcttcc gacagggcaa gttccgcgac gtcaagctcg
      901 ccaagcagca ggcggccaag ctcgacaaca tgttcgagca gatgggagag ggtgagaaga
      961 aggtcctgac cctcgtcatc aaggccgacg tgcaggggtc gcaggaggcg ctggtacacg
     1021 cgctcacgcg ccttgccacc gacgaggtga aggtcacggt ggtgcacgcc ggcgtcgggg
     1081 gtatcacgga gtccgatgtg aacctcgcca tggcttcgcg cgcagtgatc atcggcttta
     1141 acacccgcgc tgatgccacc gcacggaagc tgatcgagtc ggcgggcatc caggttcgct
     1201 actacaacat catttacgac gcagtcgagg agatcaaggc ggcgctttcc ggcatgctcg
     1261 cgcccgagcg caaggagtca gtgctcggtt tggtcgaggt gcgccaggtg ttccgcatct
     1321 cgaagatcgg cacggtggcg ggctgctacg tgcaggaggg cctggtcaag cgcagcgcgc
     1381 aagtacgcgt gctgcgcgac aacgtggtga tccacaccgg cgagatcgag tccctcaagc
     1441 ggttcaagga cgacgtgcgg gaggtcaagg ccggcttcga gtgcggcatc tcgctcaaga
     1501 acttctcgga tatcaaggaa aaggatcagt tcgaggtctt tgaggtcctg gaggtcgcga
     1561 ggactctttg aagagagggc aaggtcgtcc gcagaagctt ggcgacctga tccagcgcga
     1621 gctgtccgag ctgctgcagc gcgagctgcg tgacccgcgc gtcggcatga ttacgatcac
     1681 cggggtggac gtttcgccgg acttctccca cgccaaggtc ttcttcacgg ttctcgaaaa
     1741 gcaaaggctg gaagacgccc gcgagggcct tcggcgtgcc gccggcttcc tgcgctcgcg
     1801 gctcgcaagg cgcatcaagc tctacaccac tccggagctg cgcttcgagt acgacgagtc
     1861 ggtggagagg ggagaccggc tttcccgcct aatcaacagt gtcactggga aggcatgatt
     1921 gacggtgcct tgctcctcga caagccggtc ggggtgagct ccaacgcaac cctgcagcag
     1981 gcgaagaagc tcgtcggcgc gacaaaggcc ggccatgcgg gcacgctcga tccgctcgcc
     2041 tccgggttgc tcgtcgcgct gttcggcgag gcgaccaagt tcgccgggcc gctactcgat
     2101 gcggacaagg agtacctggc gaccctcgag ctgggcagcc gcacccgtac cggggatgcc
     2161 gagggagaga tcatggaacg aaagccggtg aacatcaccg cggccgcgct ggccgcagta
     2221 ctcgagcgct tccgcggcga aatcgagcag gtgcccccga tgcactctgc gctcaagcgc
     2281 aagggcacgc cactctaccg gctggcgcgc cggggcgagg aagtcacccg cgcggcgcgc
     2341 cgggttcgta tctctagcct ggacacggtc agtcttgaag ttcctctgct gcggatgcgc
     2401 gtgggttgca gcaagggtac ctacatccgc gtactcgctg aagatattgg cgcggcgctc
     2461 ggctgcggcg ctcatctcgc agccttgcgg cgcacgggct cgggacgttt tcgcgtcgag
     2521 aacgcgctga ctctggaagc gctggaagcg ctgccgattc acgagcgcgg gcgtgctttg
     2581 ctcgggctgc ccgcgcttgt gcacgacttg ccccgggccg agcttggcgc agcagaggaa
     2641 gcgcgcctgc gccagggcca ggcgctgaag atcagcgggc tcgagcgagg gctatgcgct
     2701 gtcgtgcggc ccgacggccg tgtgatcggg ctcggcacgg cagacggcga cggcgggctg
     2761 aagccgctgc ggctcaccca aacggctgaa aaacacttaa aatccgcggc tcatatgaaa
     2821 agagagtaga gcaatggctt tacaggttgt gcagaaggag cgggtcgtga agcaatatca
     2881 gcgctccggc aaggacaccg gctcacccga ggtgcagatc gcgctcctca ccgagcgcat
     2941 caacagcctc accgagcatt tcaagaccca cgttaaggat ttccattcgc gccgcgggct
     3001 actgaagctg gtgagccagc gccgcaagct gctcgattac ctcaagcgca aggacgcgga
     3061 caagtaccgc ggcctcatcg agcggctgag cctccgcaaa tag
//
LOCUS       DASTPD010000213         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_927069, whole genome shotgun sequence.
ACCESSION   DASTPD010000213 DASTPD010000000
VERSION     DASTPD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927069"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFR57_07095"
     CDS             complement(<1..1802)
                     /locus_tag="VFR57_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEU0259162.1"
                     /translation="MSLRITEMQQGLYHPSYEHDACGVGFVAHIKGKKSHAIIEQGLS
                     VLKNLSHRGAVGYDPKLSDGAGCLIQIPDRFYREEMARQGVKLPTAGQYGVGMVFLPR
                     DPASRLACEYEIERAIKDEGQVLLGWRDVPVDNSDLAEPAKKLEPVIRQVFIGRGRRV
                     TVTDALERKLYIIRKSSGHAIQALKLKHGKEFYVPSMSARTVCYKGMLLAYQVGEYYL
                     DLKDARVESALALVHQRFSTNTFPSWDLAHPFRMICHNGEINTLRGNVNWIRARQGAI
                     SSPILGKDLDKIWPLIYDGQSDSASFDNALELLVMGGYSVAHAMMMMIPEAWENHTLM
                     DPGRRAFYEYHAAMMEPWDGPASIAFTDGRQIGATLDRNGLRPSRYIVTDDDLVIMGS
                     ECGCLPIPEEKIVKRWRLQPGKMFLVDLERGRIVDDKELKETLADAKPYAEWIERIRV
                     KLDEVESEKTRPLKSAVPLLDRQQAFAYTQEDLKFILQPMSAAGEEPVGSMGNDSPLA
                     VLSNKNKTVYHYFKQLFAQVTNPAIDPIREELVMSLVSFIGPKPNLLGIDELNPPLRL
                     EVTQPVLDFFEMEKIRHIERYTGGKFRACELDIT"
     gene            complement(1825..2901)
                     /gene="aroB"
                     /locus_tag="VFR57_07100"
     CDS             complement(1825..2901)
                     /gene="aroB"
                     /locus_tag="VFR57_07100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0259163.1"
                     /translation="MESLRVALGSRSYPIHIGRGLISSPELYAQHIGAGGAAIVTNEV
                     VAPLYLQKVKLALQGARVAEIIVPDGEQAKTWHTLNQVFDALLSARCGRDTLIVALGG
                     GVVGDLAGFAAAVYQRGVAFLQVPTTLLAQVDSSVGGKTAINHALGKNMIGAFHQPLA
                     VISDVATLDTLPERELRSGLAEVIKHGLALDAAFFEWLESNLEKLLKKDPRALTHAVR
                     RSCELKAGVVAEDERESGARALLNFGHTFGHAIEAATGYGAWLHGEAVAAGMVIAAEL
                     SALMGYVKKTEVSRVRALLERAGLPVNGPALDPERLLELMALDKKAAKGKTRFVVLEA
                     MGRAALRADIEERAVRQAIVAAAQ"
     gene            complement(2883..3416)
                     /locus_tag="VFR57_07105"
     CDS             complement(2883..3416)
                     /locus_tag="VFR57_07105"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0259164.1"
                     /translation="MQHADNLYLVGMMGAGKTTVGRMLARRLKLRFVDSDHEIEARCG
                     VKIPVIFEIEGEAGFRAREAQAIAELVKLDGIVLATGGGVVLAAENRRLLAERGTVVY
                     LRATPEHLYERAGQDRNRPLLATGNPLARLRELYRERDPLYRSIADLIVDTGRQSVQV
                     LARGLLEQLGERWKASA"
BASE COUNT          608 a         1169 c         1152 g          647 t
ORIGIN      
        1 taggtgatgt cgagctcgca tgccctgaac ttgccgccgg tgtagcgctc gatatgccgg
       61 atcttctcca tctcgaagaa gtcgagcacc ggctgcgtca cttccagacg cagcggcgga
      121 ttgagctcgt cgatgccgag caggttcggc ttcgggccga tgaacgagac gagcgacatc
      181 acgagctcct cgcggatcgg gtcgatcgcg gggttcgtca cctgtgcgaa gagctgcttg
      241 aagtagtggt agacggtctt gttcttgttc gacagcaccg cgagcggcga gtcgttgccc
      301 attgacccga ccggctcttc gcccgcggcg ctcatcggct gcaggatgaa cttgaggtcc
      361 tcctgggtat aggcgaacgc ctgctggcgg tcgaggagcg gcaccgccga cttgagcggc
      421 cgcgtctttt cgctctcgac ctcatcgagc ttgacgcgga tgcgctcgat ccattcggcg
      481 tagggcttgg cgtccgcgag cgtctccttg agctccttgt catcgacgat gcggcccctc
      541 tccaggtcga cgaggaacat cttgcctggc tgcaagcgcc acctcttgac gatcttctcc
      601 tctggaatcg gcaggcaacc gcactccgag cccatgatca cgaggtcgtc gtcggtgacg
      661 atgtagcgcg agggccgcag gccgttgcgg tcgagcgtgg cgccgatctg ccgcccgtcg
      721 gtgaaggcaa tcgacgcggg cccgtcccaa ggctccatca tcgccgcgtg gtactcgtag
      781 aacgcgcggc gccccggatc catcagcgtg tggttctccc acgcctccgg gatcatcatc
      841 atcatcgcgt gcgccaccga gtacccgccc atcacgagca gctcgagcgc gttgtcgaaa
      901 gaggccgagt cggactggcc gtcgtagatc agcggccaga tcttgtcgag gtccttgccc
      961 aggatcggcg aggagatcgc gccctggcgg gcgcggatcc agttcacgtt gccgcgcagc
     1021 gtattgatct cgccgttgtg gcagatcatg cggaacgggt gcgcgagatc ccaggagggg
     1081 aacgtgttcg tcgagaagcg ctggtgcacg agcgcgagcg cggattccac gcgcgcgtcc
     1141 ttcaggtcga ggtaatactc gcccacctga taagcgagca acatcccctt gtagcagacc
     1201 gtgcgcgccg acatcgaagg cacgtagaac tctttcccgt gcttcagctt cagcgcctgg
     1261 atggcgtgcc ccgagctctt gcggatgata tagagcttgc gctcgagcgc gtcggtgacg
     1321 gtgacgcgcc ggccgcgacc aatgaacacc tgccggatga caggctcgag cttcttcgcc
     1381 ggctcggcga ggtcggagtt gtccaccgga acgtcgcgcc agccaagcaa tacctggccc
     1441 tcgtccttga tcgcgcgttc gatctcgtat tcgcaagcga ggcgcgaggc cgggtcgcgc
     1501 ggcaggaaca ccatgccgac gccgtactgg cccgccgtag gaagcttcac gccctgccgc
     1561 gccatctcct cgcgatagaa gcggtcgggg atctgaatca ggcagcccgc gccgtccgag
     1621 agcttggggt cgtagccgac cgcgccgcgg tggctcaggt tcttgagcac cgagagcccc
     1681 tgctcgatga tcgcgtgcga cttcttgccc ttgatgtgcg cgacgaagcc gacgccgcag
     1741 gcgtcatgct cgtaggaggg gtgatacaac ccttgctgca tctctgtaat gcgcaagctc
     1801 ataaccagac tacccaatat acatttactg cgctgcagcg acaatggctt ggcgaaccgc
     1861 gcgctcctcg atatccgcgc gcaaggcggc gcgccccatc gcctcgagca cgacgaagcg
     1921 cgtcttgcct ttggccgctt tcttgtccag cgccatcagc tcgagcaggc gctcgggatc
     1981 cagcgccggc ccgttgaccg gcaagccggc tcgctcaagc aaggcgcgca cgcggctgac
     2041 ttcagttttt ttcacgtaac ccattagcgc cgataattct gccgcgatca ccatgccggc
     2101 ggcgaccgcc tcgccgtgca gccaggcacc gtaaccggtc gccgcctcga tcgcatggcc
     2161 gaaggtgtga ccgaagttga gtagcgcccg cgcgcccgac tcgcgctcgt cctcggccac
     2221 gacacctgcc ttgagctcgc agcttcggcg cacggcatgc gtcagcgctc ttgggtcctt
     2281 cttaaggagt ttctctaggt tggattccag ccattcgaag aaagcggcgt cgagcgcgag
     2341 tccgtgcttg atcacctccg ccaagcccga gcgcagctcg cgctccggta acgtgtcgag
     2401 cgtggccacg tccgagatca ccgcgagcgg ctggtggaac gcgccgatca tgttcttgcc
     2461 gagcgcgtgg ttgatcgcgg tcttgccgcc gaccgaggaa tcgacctggg caagaagcgt
     2521 cgtcggcact tgcagaaagg cgacgccgcg ctgatagaca gcggcggcga agcccgcgag
     2581 atcaccgacc acgccgccgc ccagcgcaac gatcagcgta tcgcgtccgc agcgcgcgct
     2641 gagcagcgcg tcgaagacct ggttgagcgt gtgccaggtc ttggcttgct cgccgtcggg
     2701 aacgatgatc tccgcgacgc gcgcgccttg cagcgcgagt ttcactttct ggagatagag
     2761 cggtgcaacc acttcgttgg tcacgatggc ggcgccgccg gcaccgatat gctgcgcata
     2821 gagctccggc gacgagatga ggcccctgcc gatgtggatc ggataggagc ggctgccgag
     2881 cgctacgcgg aggctttcca tcgctctccg agctgctcga gcaggccgcg cgccagcact
     2941 tgcacgctct gccggccggt gtccacgatc aagtcggcga tactgcgata cagcggatcg
     3001 cgctcgcgat aaagctcgcg caggcgcgcg agcggattgc cggtcgcaag caacgggcgg
     3061 ttgcggtctt gccccgcacg ctcgtagagg tgctcgggcg ttgcgcgcag gtacactacc
     3121 gtgccacgct cggcgagaag ccgccggttc tcggccgcga gcacgacgcc tccgccggtg
     3181 gcgagcacta tgccgtcgag cttgacgagc tccgcgatcg cctgcgcctc gcgcgcgcgg
     3241 aaaccagcct ctccctcgat ctcgaagatc accgggatct tcacgccgca gcgtgcctcg
     3301 atctcgtgat ccgaatcgac aaagcgcaac ttcaggcgcc gcgcgagcat tcgcccgacg
     3361 gtggtcttgc cagcgcccat catgccgacg aggtacaggt tgtccgcgtg ctgcattccc
     3421 gcattgtaga aacaaaagcg gccgaggggt cccggccgcg cctgctcaaa agaggaagga
     3481 gaagggggcg atgctagcga atggtgagcc gctcattaac cacgcgcggt gtgatgaaca
     3541 cgaggagctc ggttttcgag gtgctcgagg tcgtgt
//
LOCUS       DASTPD010000214         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_724855, whole genome shotgun sequence.
ACCESSION   DASTPD010000214 DASTPD010000000
VERSION     DASTPD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724855"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFR57_07110"
     CDS             complement(<1..248)
                     /locus_tag="VFR57_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0259165.1"
                     /translation="MMRLIIAALVLALSACASHKADETKRTTTEFTSDAALTARVKSA
                     IATDVGARTAAAINIETYRGVVQLTGFVSSPEQAQRAEA"
     gene            complement(343..540)
                     /locus_tag="VFR57_07115"
     CDS             complement(343..540)
                     /locus_tag="VFR57_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU0259166.1"
                     /translation="MNWDRIQGNWKQMSGKLKEKWGKLTDDEFDQMAGNRDQLVGKIQ
                     ERYGIQKDEAERQVKDWESRM"
     gene            complement(586..1173)
                     /locus_tag="VFR57_07120"
     CDS             complement(586..1173)
                     /locus_tag="VFR57_07120"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0259167.1"
                     /translation="MDANHNPSLMIWHAKPDMSCEYVNQAWREFTGYTLEEARGGGWY
                     RALHPDDLTRWLDASVRAFDERRPFEIEYLMRRRDGEYRWILDRAAPRYGAGGAFLGF
                     GGTSVEIDAGRRGASLAGLRVLVVGHDRGTFEELVRPLEVAGADVRVAAGGSEALLTL
                     DSWHPDVLLSESSEIRALRELPEDELLDAVAQLAA"
     gene            1366..1530
                     /locus_tag="VFR57_07125"
     CDS             1366..1530
                     /locus_tag="VFR57_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0259168.1"
                     /translation="MLSWALAFFIIAIIAAVFGFTGIAAGAAEIAKILFVVFVVLFLV
                     SLVAGLLRRP"
     gene            1544..2026
                     /locus_tag="VFR57_07130"
     CDS             1544..2026
                     /locus_tag="VFR57_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259169.1"
                     /translation="MKSKLTGIALAVAFAFAGTAQAQKSERKATHAEKEQIEAAYKAD
                     KAKCDAMKANAKDICMAEAKGKQKVAKAELDAKNEKDQAKAQKKVQDAKAEATYDVAK
                     EKCDDQKGEAKDACMKQARVERDRAKGKAEKTADAKRDNAKEHRAATGGTAPERKPAK
                     "
     gene            2077..>2243
                     /locus_tag="VFR57_07135"
     CDS             2077..>2243
                     /locus_tag="VFR57_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0259170.1"
                     /translation="MARGLWKGAISFGLVNVPVELHSAKKRSAELDMTMLDKRDLAPV
                     GYKRVNKSTGK"
BASE COUNT          392 a          753 c          736 g          362 t
ORIGIN      
        1 gcctcggcgc gctgcgcctg ctcaggcgag gacacgaagc cggtgagctg gaccacgccg
       61 cggtaggtct cgatgttgat cgcggccgcg gtgcgcgcgc ccacgtcggt ggcgatcgcg
      121 ctcttcaccc tggcggtgag cgcggcgtcg ctggtgaatt cggtggtggt gcgcttggtc
      181 tcgtccgcct tgtgcgacgc gcaagccgag agcgcgagca ccagcgcggc gatgatcagc
      241 ctcatcatga cttgtccttc tcgggaaccc gcaccggctc gtggtcgccc ggctgttgcg
      301 ggtcgaacgg ccacttcgcc ggttgcggct tctccttctc cattacatcc tcgattccca
      361 gtccttcacc tggcgctcgg cctcgtcctt ctggatgcca tagcgctcct ggatcttgcc
      421 gaccagctgg tcgcggttgc ccgccatctg gtcgaactcg tcgtcggtca gcttgcccca
      481 cttttccttc agcttgccgc tcatctgctt ccagttgcct tgaatgcgat cccagttcat
      541 ttcagtgagt ctccttagaa agatgcgaaa gggttcgagc aaaactcatg ccgccagctg
      601 cgccacggcg tcgagcagct cgtcctccgg cagctcgcgc agcgcgcgga tttcgctgct
      661 ctcggacagc agcacgtccg gatgccatga atcgagggtc agcagggcct cggagccgcc
      721 ggccgccacg cgcacgtcgg cgcccgccac ttccagcggg cgcaccagct cctcgaaggt
      781 gccgcggtcg tgccccacga ccagcacgcg cagcccggcg agcgaggcgc cgcgccggcc
      841 ggcgtcgatc tcgacgctgg tgccgccgaa gccgaggaac gcgccgcccg cgccgtagcg
      901 cggcgccgcg cggtcgagga tccagcggta ctcgccgtcg cgcctgcgca tgaggtactc
      961 gatctcgaag ggacggcgct cgtcgaacgc gcgcacgctc gcgtcgagcc agcgcgtgag
     1021 gtcgtcggga tgcagcgcgc ggtaccagcc gccgccgcgc gcttcttcca gcgtgtaacc
     1081 ggtgaactcc cgccacgcct ggttgacgta ttcgcacgac atgtccggct tcgcgtgcca
     1141 gatcatgagc gatgggttgt ggttcgcgtc catgccattc ggatcaaggt aatcgcaacg
     1201 cagcaggagc cgtgcccgta gcgggatcac tacgacgcgc gcccttgagc gacacgtagt
     1261 tcttacagca cgctcccgcg cgttacaccg gcgcgcgcgg cggcgccgcg gcatggagtt
     1321 tgctcacgcc tctgtgctgc aaccactttc tttaaggagt tcgtcatgtt gagttgggcg
     1381 cttgcgtttt tcatcatcgc catcatcgcg gcggtgttcg gcttcaccgg catagccgcc
     1441 ggcgcggccg agatcgccaa gatcctgttc gtcgtgttcg tggtgctctt cctcgtctcg
     1501 ctggtcgcgg gactgctgag aaggccctaa ccaaggagtc cccatgaaaa gcaaactcac
     1561 cggtatcgcc ctggccgtgg cattcgcgtt cgccggcacg gcgcaggccc agaagtccga
     1621 gcgcaaagcc acccacgcgg agaaggagca gatcgaagcc gcgtacaagg ccgacaaggc
     1681 caagtgcgac gccatgaagg cgaacgccaa ggacatctgc atggccgaag ccaagggcaa
     1741 gcagaaagtc gcgaaggcgg agctcgacgc gaaaaacgag aaggatcagg ccaaggcgca
     1801 gaagaaggtg caggacgcca aggccgaggc cacctatgac gtcgcgaagg agaagtgcga
     1861 cgaccagaag ggcgaggcca aggacgcgtg catgaagcag gcgcgcgtgg agcgcgaccg
     1921 cgccaagggc aaggcggaga agaccgccga cgccaagcgc gacaacgcca aggaacaccg
     1981 cgccgccacc ggcggcacgg cgcccgagcg caagccggcg aagtaacgat cgtcgatccc
     2041 tcccggggcg cagccgcccc ggggacttct tttcccatgg cgcgcggact ctggaaaggg
     2101 gcgatcagct tcgggctggt caacgtcccg gtcgagctgc attcggcaaa gaagcgctcg
     2161 gccgagctcg acatgacgat gctcgacaag cgcgacctcg cgccggtcgg gtacaagcgc
     2221 gtcaacaagt cgaccgggaa gga
//
LOCUS       DASTPD010000215         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1337311, whole genome shotgun sequence.
ACCESSION   DASTPD010000215 DASTPD010000000
VERSION     DASTPD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337311"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VFR57_07140"
     CDS             <1..1845
                     /locus_tag="VFR57_07140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU0259171.1"
                     /translation="NAVDEALAGFGKRITVSVFKDGSVQVEDEGRGIPVDMHPVEKKP
                     AVEVAFTMLHAGGKFSRSGDGVYHISGGLHGVGVSVSNALSKRCEVVVFRESQKHAIT
                     FEGGEVKNKLRSERIKEKKTGTIVRLWPDARYFDSPNIPMNELEHLVRSKAYLLPGLT
                     MALEVEGKQTRLWKYERGMAQYFEFMLDGRELVAPLFTGERFFDEKSLSGVTDIEPGE
                     GASWAIGWVAEGDIFADSHVNLIPTRSGGTHEAGFRAGIFEALGAFMDARGLAPKGVK
                     LIADDLWQRACFTLSARIVRTQFHGQTKEKLTTRHAARLLELCVRDAFELWLNEHADE
                     GKKIVELAIEQAMARLAKGKKVERKKSSGIATLPGKLVDCASGDISRNELFLVEGDSA
                     GGSAKEARDKETQALLPLRGKVLNSMSKDSRTVLANKELQAIATAIGVDPHGVDEKAD
                     LSSLRYGKIIVMTDADVDGGHIQALLLTFFFMHAPRLVEAGHLHVAQPPLYKVEVPSQ
                     GKGKPARRIYCLDDDELEEVLNQFKKEKIKDGSWEISRFKGLGEMSPEQLWETTMNPD
                     ARRLVRMEFDTRNMKKVRETFEKLMDAGEAEERRNWMREHWKTVEADI"
     gene            1829..2065
                     /locus_tag="VFR57_07145"
     CDS             1829..2065
                     /locus_tag="VFR57_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259172.1"
                     /translation="MKRTSRKNKPFWERAYKGHVLWLGKRKLGKVNLTNPDQRGKYQW
                     EAAGRAGFADELEKAKAAVELAVSTADKQLDLFR"
     gene            2072..>3547
                     /locus_tag="VFR57_07150"
     CDS             2072..>3547
                     /locus_tag="VFR57_07150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEU0259173.1"
                     /translation="MDDNRTGDLFTSNGDDAARIEEHASHAYLGYAVSTVKARALPEI
                     ADGLKPVQRRILFAMNGSTTFSKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSMRY
                     PLIDGQGNFGSRDGDAPAAYRYTEARLSRYAELLLSEIGEGTVDFIRNYDGKFDEPVL
                     LPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAHIIKHPRAKLEDVRKILPGPD
                     FPGGGQVISPKEEIEEAYATGRGSLLLRARYEKETLARGQWRMVVTELPHGVSVKQVM
                     EEIEALANPKSVSKKEVSQEQRRMKQFILDQVESVRDESDRKSKLRLVIEPRSSRQDE
                     NELMAALMVHTSLETRYPVNLTWLGLDGLPQTKGIVDILSEWGEFRVRTVRRRTEFRL
                     AKCEARLHIVEGRLKAFARIDDIIKLIRAAEDQAEARSNLQKRFKFSERQAEDIVNLR
                     LGQLTRLDGIALNDERKALEAERKGLKDILGEEKELKKLVVA"
BASE COUNT          733 a         1084 c         1195 g          535 t
ORIGIN      
        1 aacgcggtcg acgaggcgct cgccgggttc ggcaagcgca tcaccgtgag cgtgttcaag
       61 gacggctcgg tgcaggtcga ggacgaaggc cgcggcatcc cggtcgacat gcacccggtg
      121 gagaagaagc ccgcggtcga ggtcgcgttc accatgctgc acgccggcgg caagttctcg
      181 cgctcggggg acggcgtcta ccacatctcc ggcggcctgc acggggtcgg cgtctcggtg
      241 tcgaacgcgc tttcgaagcg ctgcgaagtt gttgttttta gagaaagcca gaaacacgcc
      301 atcacgttcg aaggcgggga ggtcaagaac aagctccgct cggagcgcat taaggagaaa
      361 aagaccggca ccatcgtgcg cctctggccg gatgccaggt atttcgattc gcccaacatc
      421 ccgatgaacg agctcgagca cctggtgcgc tcgaaggcgt acctgctgcc cgggctgacc
      481 atggcgctcg aggtggaagg aaagcagacg cgcctgtgga agtacgagcg cgggatggcg
      541 cagtacttcg agttcatgct cgacggccgc gagctcgtcg cgccgctttt tacgggcgag
      601 cgcttcttcg acgagaagtc gctctcgggg gtgacggaca tagaaccggg cgagggcgca
      661 tcgtgggcga tcggctgggt cgcggagggc gacatcttcg ccgacagcca cgtgaacctg
      721 atcccgacgc gctcgggcgg cacgcacgag gcgggcttcc gcgcggggat ttttgaggcg
      781 ctcggcgcgt tcatggacgc gcgcggcctc gcgccgaagg gcgtcaagct catcgccgac
      841 gacctctggc agcgcgcctg ctttacgctc tcggcgcgca tcgtgcgcac gcagttccac
      901 ggccagacca aggaaaagct caccacgcgc cacgccgcgc gcctgctcga gctgtgcgtg
      961 cgcgacgcgt tcgagctctg gctgaacgag cacgccgacg agggcaagaa aatcgtcgag
     1021 ctcgcgatcg agcaggcgat ggcgcgcctc gccaagggca agaaagtcga gcgcaagaag
     1081 tcgagcggca tcgccacgct tcccgggaag ctcgtcgact gcgcttcagg cgatatttcc
     1141 cgaaatgaat tgttcctggt ggaaggtgat tccgcgggag gatcggcgaa ggaagcgcgc
     1201 gacaaggaaa cgcaggcgct gcttccgctg cgcggcaagg tgctgaactc gatgtcgaag
     1261 gacagccgca cggtgctcgc gaacaaggag ctgcaggcca ttgccacggc gatcggagta
     1321 gacccccacg gggtcgacga aaaagctgac ttgagttctc tgcgctacgg caagatcatc
     1381 gtcatgacgg atgcggacgt cgacggcggc cacatccagg cgctgctcct cacgttcttc
     1441 ttcatgcatg cgccgcgcct tgtggaggcc ggccacctcc acgtcgcgca gccgccgctc
     1501 tacaaagtcg aggtgccgag ccagggcaaa ggcaagcccg cccggcgcat ctactgcctg
     1561 gacgacgacg agctggaaga ggttctgaat cagttcaaga aagaaaaaat caaggacggg
     1621 agctgggaaa tctcgcgctt caaggggctg ggcgagatga gcccggagca gctctgggaa
     1681 accaccatga acccggacgc gcggcggctg gtgcgcatgg agttcgacac caggaacatg
     1741 aagaaagtga gagagacctt cgagaagctg atggacgccg gcgaggccga ggagcggcgc
     1801 aactggatga gggagcactg gaagaccgtt gaagcggaca tctaggaaga acaagccctt
     1861 ctgggaaagg gcctacaaag gccacgtgct gtggctcggc aagcgcaagc tcggcaaagt
     1921 caatttgacc aacccggatc agcgcggcaa gtaccagtgg gaagcggccg gcagggcggg
     1981 cttcgccgac gagctcgaga aggcgaaagc cgcggtcgag ctcgccgtct ccacagcgga
     2041 caaacagctg gatcttttca ggtaaggccc catggacgac aaccgcaccg gagatctctt
     2101 caccagcaac ggcgacgacg cggcacgcat cgaggagcac gcctcgcacg cgtacctcgg
     2161 ctatgcggtc tcgaccgtca aggcgcgcgc gctgccggag atcgccgatg ggctgaagcc
     2221 ggtgcagcgc cggattctgt ttgcaatgaa tggttcaacc accttctcga aatgcgcgcg
     2281 ctacgtcggc gaggtgctcg gcaagtacca cccgcacggc gacagctcca cgtatgaagc
     2341 gctggtgcac ctcgcgcagc ccttctcgat gcgctatccc ctcatcgacg ggcaggggaa
     2401 ctttggttcc cgtgatggcg atgcgcccgc tgcctaccgg tataccgagg cgcggctctc
     2461 gcgctacgcc gaattgctgc tctccgaaat cggggagggc accgtcgact tcatcaggaa
     2521 ctacgacggg aaattcgacg agccggtgct gctgccggct cgattgccct tcggcttgct
     2581 gaacggaagc ttcgggatcc cggtcggctt ctccacccgc atcccgtcgc acaacctgaa
     2641 ggaagtcgcc gccgcggcgg cgcacatcat caagcacccg cgcgcgaagc tcgaggacgt
     2701 gcgcaagatc ctgcccgggc cggacttccc cggcggcggc caggtgatct cgcccaagga
     2761 ggagatcgag gaagcctacg ctaccgggcg gggctctttg ctcctgcgag cgagatatga
     2821 aaaggaaacg ctcgcgcgcg ggcagtggcg catggtggtc accgagctcc cgcacggcgt
     2881 gtcggtgaag caggtgatgg aagagataga ggcgctcgca aaccccaagt ccgtatccaa
     2941 gaaagaagta tctcaggaac aacgcaggat gaagcagttc atcctcgacc aggtcgagtc
     3001 ggtgcgcgac gagagcgacc gcaagtcgaa gctgcgcctg gtgatcgagc cgaggagctc
     3061 gcgccaggac gagaacgagc tcatggccgc gctgatggtg cacacctcgc tcgagacgcg
     3121 ctacccggtg aacctcacct ggctcggcct cgacggcctg ccgcagacca agggcatcgt
     3181 cgacatcctg agcgagtggg gcgagtttcg cgtgcgcacg gtgcgccggc gcaccgagtt
     3241 tcgcctcgcg aagtgcgagg cgcgcctgca tatcgtcgag gggcggctga aggccttcgc
     3301 caggatcgac gacatcatca agctcatccg cgcagccgag gaccaggccg aagcgcgctc
     3361 gaatttacag aagcggttca agttttcgga aagacaggcc gaagacatcg tcaacctgcg
     3421 cctcgggcag ctcaccaggc tcgacggcat cgcgctgaac gacgagagga aggcgctcga
     3481 agccgagcgc aaggggctga aggacatcct cggcgaggag aaggagctga agaagctcgt
     3541 cgtggcc
//
LOCUS       DASTPD010000216         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_520605, whole genome shotgun sequence.
ACCESSION   DASTPD010000216 DASTPD010000000
VERSION     DASTPD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520605"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFR57_07155"
     CDS             <1..433
                     /locus_tag="VFR57_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259174.1"
                     /translation="PLLYALYGAARIANTIGIYRRRLETLDLREWDRAIRSGKVRLSA
                     FASLAVDLRSTPLAAYVRMLAATVEPWPEAGKIAVPVLALLSTQSSFTDLVLTRRALG
                     ALPDCEVVELDAVHWIPTEQPDAMRAAIEDWLARRAPGKAP"
     gene            complement(405..1034)
                     /locus_tag="VFR57_07160"
     CDS             complement(405..1034)
                     /locus_tag="VFR57_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU0259175.1"
                     /translation="MSSWQEYLRFVVTLTAVLDPFLAVPIFVSVTTGERSAAKQRLTN
                     VVTLTVFLVLAGSALFGELLLRLIGASLPAFQVGGGLVLLLMALAMLNAQAGEIRQTR
                     AEARELRSREYSGVVPLAVPLLAGPGAISTTIIAAQAGGFLHGAVLALCVAFVCALLW
                     VVLRLAHAIAGRMGTTGLNIATRLLGLLLAAIAIQTMAEGLRVLFPGLA"
     gene            complement(1031..1681)
                     /locus_tag="VFR57_07165"
     CDS             complement(1031..1681)
                     /locus_tag="VFR57_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0259176.1"
                     /translation="MLRLCGFHVSNYHNKVRIALLEKDVPFEEDPTCTPSQKDGFVAR
                     SPMGKVPFLELDGGRRLAESEVILEYLEDAYPQKPLLPKDPFERAKVRELVTVTELHL
                     ELPTRRLYGHVFFKKPASEEVIRAVEKDLAKGVRALRTLVKFDPYIAGGEVTIADCAA
                     FVHLPLVSLTTKLAYGRDILEDLAPLKPYLKMLGERPAFRRVNEDRKTAQAALAKK"
     gene            complement(1706..2512)
                     /locus_tag="VFR57_07170"
     CDS             complement(1706..2512)
                     /locus_tag="VFR57_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0259177.1"
                     /translation="MLTLYHHNVSVCSQKVRLQLEEKKIPWRGEHVDLLRGDHLKRQF
                     LRLNPRGLVPALVHDQAVIIESMVILEYIEDVFPEPALRPSDPALRAQMRVWQKLPDD
                     GLHAACGTISFAAAFAQQLARGVDGDDLERRLSKIPDPERADRQRTLIREGFNAPILR
                     QAVGVYERALDEMNLALQKPDALFLVGTRFTLAEVALAPYMERLHRLGLGGLWEHARP
                     HVTSWFARLRGRPSFKAAFEAYAPNGYDDLVRQKGVDLWPRLELLLRKAS"
     gene            complement(2522..3493)
                     /locus_tag="VFR57_07175"
     CDS             complement(2522..3493)
                     /locus_tag="VFR57_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259178.1"
                     /translation="MFIRIVRLLFGMAVFGFAMPAVAQEDWPNRPIKFVVPTPPGGGT
                     DLYARALAPALSEQLKQPAIVENRPGAGGNIGADAVARAAPDGYTFLVSATGTMVVNP
                     SLYRELPFDVERNFTPVARGVTGPFAFVSHPAFVAKNLNELVALARSKPGQISFGSAG
                     VGSVSYLAVRMLEEEAGVSFLHVPYKGLGQAYQDLLGGQLDFVYSDLGLALPHIQAGR
                     MHPLAVTAKTKLIPQAPTLGDAGFKDVRADNFFSVYAPAGTPTSIIDRLNAAIGSAMR
                     SPTLAAKLEAQGWTPVFETPAQFAASVKRERKRWAAFIRRNGITADQ"
     gene            complement(3597..4523)
                     /locus_tag="VFR57_07180"
     CDS             complement(3597..4523)
                     /locus_tag="VFR57_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0259179.1"
                     /translation="MKLDLNLLAIFDAVLRTGSVTRAADLVGLSQSSTSNALHRLRHA
                     LGDPLFIRCGNRMIPTPFAAAIAHPIQDALNSVADALQGARAFDPRRSDRTFRIAMND
                     LGQLRFMPKLIAHAREVAPGITLETVDIDPELVKHSLAEGAIDLAIGPLHDFGPQYHR
                     WRLFNDTLVCVVSAQHSSIRDTLTLDAYLGGVHGAYRPAAASHAFINRAVEAVFLAHR
                     RERRVAVRLAYTIGLGPIIESSDLIFTLPGGLAARIARGAKLRVLPTPFKAPRFEISQ
                     QWHHRFHKDPGNEWLRKSVAKLFLNADPHAFY"
     gene            complement(4608..>5045)
                     /locus_tag="VFR57_07185"
     CDS             complement(4608..>5045)
                     /locus_tag="VFR57_07185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0259180.1"
                     /translation="AVKRGYHMHYRTAGRAVLNHPMLDTERGYFLAPMRRGIRLTTGA
                     EFALRDGMKTPVQLNRAEPIARELFPLAERLDPDPWMGSRPCTPDMMPVIGRAPRHEK
                     LWFAFGHAHHGFTLGPVTGRLVAELVCGEPPFVDPRPYRAERF"
BASE COUNT          898 a         1624 c         1660 g          863 t
ORIGIN      
        1 accgctgctc tatgcgctct atggcgcagc gcgtatagcg aacacgatcg gcatctaccg
       61 ccgacgcctc gagaccctgg atctgcgcga atgggaccgg gccatccgca gcggcaaggt
      121 ccggctgtcg gcgttcgctt cgctcgcggt cgatctaagg tcgacgccgc tcgccgccta
      181 tgtgcgcatg ctcgccgcca ccgtggagcc gtggcccgag gcggggaaga tcgccgtgcc
      241 ggtgctcgca ctgctttcca cgcaaagcag cttcaccgat ctggtactca cgcgccgggc
      301 gctcggcgca ttgcccgact gcgaggtggt cgaactggac gcggtgcact ggatccccac
      361 cgagcaaccc gacgcgatgc gcgccgcgat cgaagattgg ctcgctaggc gagccccggg
      421 aaaagcaccc tgaggccttc cgccatcgtc tggatcgcga tggcggcgag cagaagcccg
      481 agcagccgcg tcgcgatgtt gaggcccgtg gtgcccatgc gccccgcgat agcgtgcgcg
      541 aggcgcagca ccacccagag cagcgcgcag acgaacgcga cgcatagcgc gagcaccgcg
      601 ccgtgcagga agccgcccgc ctgcgcggcg atgatggtgg tgctgatcgc gcccggcccg
      661 gcgaggagcg gcaccgcgag cggcaccacg cccgaatact cgcgcgacct gagctcgcgc
      721 gcctcggcgc gtgtctggcg gatctcgccc gcctgcgcgt tcagcatcgc gagcgccatg
      781 aggagcagca ccaggcctcc gccgacctgg aacgcgggca ggctcgcgcc gatcaggcgc
      841 agcagcagct cgccgaacaa cgccgatccg gccaggacca ggaacactgt cagcgtgacg
      901 acattggtga gccgctgctt ggcagccgaa cgctcgccgg tggtgacgct cacgaagatc
      961 ggcaccgcga gaaatggatc cagcaccgcg gtcagcgtga cgacgaagcg caggtattcc
     1021 tgccaggagc tcacttcttg gcgagcgcgg cttgcgcggt cttgcggtcc tcgttgaccc
     1081 tgcggaacgc agggcgctcg ccgagcatct tgagataggg cttgagcggc gcaaggtcct
     1141 cgagaatgtc gcgcccgtag gcgagcttgg ttgtcagcga aacgaggggc aggtgcacga
     1201 acgccgcgca atcggcgatg gtcacctcgc cgcccgcgat gtaggggtcg aacttcacca
     1261 gcgttctcag cgcgcgcacg cccttggcca ggtccttctc gaccgcgcga atgacttctt
     1321 cggatgcggg tttcttgaag aacacatggc catagagccg gcgcgtcggc agctccaggt
     1381 gaagctccgt gacggtgacg agctcgcgca ccttggcgcg ctcgaacggg tccttgggca
     1441 ggagcggctt ctgcgggtac gcatcctcga gatactcgag gatcacttcg gactcggcga
     1501 gccgccggcc gccgtcgagc tcgaggaacg gcaccttgcc catcggcgag cgcgccacga
     1561 acccgtcctt ctgcgagggc gtgcaggtcg gatcctcctc gaacggaacg tccttctcaa
     1621 gtagcgcaat acggaccttg ttgtgatagt tgctgacgtg gaaaccgcag agtcgtagca
     1681 tcaaagcatt ataggcatcg cactgtcacg acgcttttcg gagcagcaac tccagtctcg
     1741 gccacaggtc gacgcctttc tgtctgacta agtcgtcgta gccgttcgga gcgtaggctt
     1801 cgaacgccgc cttgaagctg gggcggccgc gaaggcgggc aaaccagctg gtcacatgcg
     1861 gtctagcgtg ttcccatagg ccgccgagcc caaggcggtg cagccgctcc atgtaaggcg
     1921 ccagcgcgac ttccgccaag gtgaaccgcg tgcctacgag aaagagggca tcgggcttct
     1981 gcagcgcaag gttcatctcg tcgagcgcgc gctcatacac gccgacggct tggcgcagga
     2041 tcggcgcgtt gaacccctcc cttatgaggg tccgttgccg atctgcccgc tccggatcgg
     2101 gtattttcga caggcggcgc tcgaggtcgt ccccgtcgac tccccttgcc agttgctgag
     2161 cgaacgcggc cgcgaagctt atcgtcccac atgcggcatg caggccgtcg tccggcaatt
     2221 tttgccatac cctcatctgt gcgcgcagtg ccgggtctga cggacgcagt gcgggctccg
     2281 gaaacacgtc ctcgatgtat tcgaggatga ccatggattc gatgatcacc gcctggtcgt
     2341 gcacgagcgc tggcaccagc ccgcgcgggt tgagccgcag gaactgcctc ttcagatgat
     2401 ctcctcggag caggtcgaca tgctctccgc gccagggaat cttcttttct tccagctgca
     2461 gtcgcacctt ctgcgagcag accgaaacgt tatggtggta aagggtaagc agatcgagcc
     2521 cttattgatc tgccgttatg ccatttcggc ggatgaacgc ggcccaccgc ttgcgctcgc
     2581 gcttgacgct cgcggcaaat tgcgccggcg tttcgaacac cggggtccat ccttgcgctt
     2641 ccagctttgc ggcgagcgtc ggcgagcgca ttgcgctgcc gatcgcggcg ttcaggcgat
     2701 cgatgatgga cgtcggcgtg cccgccggcg cgtatacgct gaagaagttg tctgccctaa
     2761 catctttgaa accggcgtcg cccaacgtcg gggcttgcgg aatcagtttc gtctttgcgg
     2821 tgaccgccag gggatgcatc cttcccgcct gaatgtgcgg caaagccaag ccgagatcgc
     2881 tatatacgaa atcgagctgg ccgccgagca gatcctggta cgcctggccc agccccttgt
     2941 atggaacgtg caggaagctc acgcccgcct cctcctcgag catccgcacc gcaagatagc
     3001 tgacgctgcc cacacccgcc gacccgaaag agatctggcc cggcttactg cgcgcgaggg
     3061 caaccagctc gttgagattc ttggcgacga acgccggatg cgagacgaat gcgaagggcc
     3121 cagtcacccc gcgcgccacc ggagtgaaat tgcgctcgac gtcgaagggc agctcgcggt
     3181 acaggcttgg attgaccacc atggtcccgg tggccgaaac aagaaaggtg tatccgtccg
     3241 gcgcggccct agccaccgca tctgcgccga tattgccgcc ggctccgggg cgattctcta
     3301 cgatcgccgg ctgcttcagc tgctcgctca gcgccggtgc gagcgcgcgc gcgtagaggt
     3361 cggtgccacc gccgggcggt gtgggcacga cgaacttgat tggccggttt ggccagtcct
     3421 cctgcgccac cgctggcatg gcaaagccga agacagccat accgaacagg agacgaacaa
     3481 ttcttatgaa catggcgcat tcctcctttc gacgcccaac ggatcgggtt cgccgagcgt
     3541 agttcggaag aagcggtcgg tcgacgccgc gccgtcgcta catgttattg acgggatcaa
     3601 tagaacgcgt gcgggtctgc attcaggaac agtttcgcga ccgacttacg tagccactcg
     3661 ttgcctggat ccttgtggaa gcgatggtgc cactgctggc tgatctcgaa cctgggcgcc
     3721 ttgaacggcg tgggcagcac gcgaagcttg gcgcccctcg caatacgcgc ggcaagaccg
     3781 cccgggagag tgaagatcag gtctgaagat tcgatgatcg ggccgagtcc gatggtgtat
     3841 gcaaggcgta cggctacacg gcgttcccgc cgatgcgcaa ggaatacggc ctcgaccgca
     3901 cggttgatga atgcatggct cgcagcggcc ggccggtaag ccccgtgcac gccgccgagg
     3961 taagcgtcca gcgtcagggt gtcgcggatg cttgagtgct gcgccgaaac gacacaaacc
     4021 aacgtgtcgt tgaacaggcg ccaccggtga tattggggac cgaagtcgtg cagcggtcct
     4081 atggccagat ctattgcgcc ttcggccagg gaatgcttca cgagctctgg atcgatatcc
     4141 accgtctcga gcgtaatgcc cggagcgacc tcgcgcgcat gcgcgatcaa cttgggcatg
     4201 aagcgcagct ggccgagatc gttcatcgct atacgaaatg tcctgtccga ccgacgggga
     4261 tcgaaggcgc gtgccccttg cagggcgtca gcgacgctgt tcaacgcgtc ctgaattgga
     4321 tgcgcgatcg ccgcggcaaa cggcgtcggg atcatccggt tgccgcagcg aatgaaaagc
     4381 ggatcgccga gcgcatgcct gagccggtgc agcgcattgc tcgtcgaaga ctggctcagc
     4441 ccgaccagat cagcggctcg tgtcacgctg ccggtacgaa ggacggcatc gaagatggca
     4501 agcagattaa ggtcgagctt cacggcccct ccctggaatc cgaagcacat cgtatccggg
     4561 gcggatcgcg atgcgtgttc cgcgcccttt ggcactgcaa atgcgcccta aaacctctcg
     4621 gcgcgatacg gtcgcgggtc gacgaagggc ggctcgccgc acacgagctc ggccaccagg
     4681 cgcccggtga ccgggccgag ggtgaagccg tgatgcgcgt ggccgaaggc gaaccagagc
     4741 ttttcgtgcc ggggcgcgcg cccgatgacc ggcatcatgt ccggggtgca cgggcgcgag
     4801 cccatccagg ggtctgggtc gagccgctcg gcgagcggga agagctcgcg cgcgatcggc
     4861 tcggcgcggt tgagctgcac cggggtcttc atgccgtcgc gcaacgcgaa ctccgcgccc
     4921 gtggtgaggc ggatgccgcg ccgcatcggc gcgaggaagt agccgcgctc ggtgtcgagc
     4981 atcgggtggt tgagcaccgc gcggcccgcg gtacggtagt gcatgtggta gccgcgcttc
     5041 accgc
//
LOCUS       DASTPD010000217         5498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1133680, whole genome shotgun sequence.
ACCESSION   DASTPD010000217 DASTPD010000000
VERSION     DASTPD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133680"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VFR57_07190"
     CDS             complement(<1..160)
                     /locus_tag="VFR57_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0259181.1"
                     /translation="MSYRLIVFDWDGTIIDSAATIAECIRDAARELELEVPDLERARH
                     VIGLGLHDA"
     gene            complement(157..1074)
                     /locus_tag="VFR57_07195"
     CDS             complement(157..1074)
                     /locus_tag="VFR57_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0259182.1"
                     /translation="MNDLRKARATTIEVGEEAEAQRIDNFLLRALKGVPKSHVYRVLR
                     SGEVRVNSGRVKPDYRLQPGDRVRIPPVRTAERKPALPRPLDLPVVFEDSALLVIDKP
                     SGLAVHGGSGVSFGVIESMRAARPQARSLELAHRLDRDTSGLLILCKKRSALTELHRM
                     LREGEVRKVYFALVKGEVTEKTLELRESLHKYVTGSGERRVSVREGGLAALTRARRLK
                     ASMEFSLLEVELLTGRTHQIRVHLAHAGYPIVGDDKYGDFALNKVWKQRLQLHAGKLG
                     FRHPLTGQPVKLESPLPEDMKRFVSEVLR"
     gene            1326..3887
                     /locus_tag="VFR57_07200"
     CDS             1326..3887
                     /locus_tag="VFR57_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU0259183.1"
                     /translation="MKRMLFNATQQEELRVAIVDGQKLVDLDIESASREQRKSNIYKG
                     IITRIEPSLEAAFVDYGEERHGFLPFKEVSRSYFKEGVDVGRARIQDALREGQEIIIQ
                     VEKDERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTLDQLQLP
                     DGMSVIARTAAIGRSLEELNWDLNYLLQLWAAVESASQDQSKKPFLIYLESSLVIRAI
                     RDYFQPDIGEILIDTEEIYEQAKSFMQTVMPGNVGKVKLYRDDIPLFSRFQIEHQIES
                     AYSRQVALPSGGAIVIDHTEALVAIDVNSARATRGGDIEETAFRTNLEAAEEVARQLR
                     LRDLGGLIVIDFIDMESQRNQREVENRLRDSLRYDRARVQIGKISRFGLMELSRQRLQ
                     ASLEETAHISCPRCSGTGFIRGTESTALHVLRIIQEEAMKENTGAVHAQVPVDVSSFL
                     LNEKRAEIQKLEARLKVNIVLVPNPHLETPHYKVQRLKHDELNQMEHVPASYELVEQP
                     EEPKAPGADEETKRERQEAVVKGITPAQPAPMVDHSAPRPPRMPEPQPVPQAVAPAPV
                     LEGGFLSKIISLFKPVAAPAAQPQPAAQPAPQRAQEPRRDHQQRRDHQQRRDGRDRGR
                     DGRPREENRDRQQQGRLEQRRGNGQPRGERPNRGERGERGERRPDQQRPPRPPHQQPQ
                     QAQPQSQPQPQQQSAPRPPEARNGDGEPRRGGRRNRGRDRQRERHDGRPAQGERAPNG
                     APSFTLKEDQLREDQRREALARKEELAAEETLPPPALAPAPRMEEPKREPRPEPKIDP
                     RPILESEGLVMIETDRAKAAYVPIEDQPQQLGRPRRDRPKLAPQEEQLEQVETRK"
     gene            complement(3877..4332)
                     /locus_tag="VFR57_07205"
     CDS             complement(3877..4332)
                     /locus_tag="VFR57_07205"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEU0259184.1"
                     /translation="MKERRSILFVCTGNICRSPTAEGVLRHLASERSLELRIESAGVG
                     DWHIGDPPDERALRHAKSRGYDLSAQRARQVRAQDFEEFDMILAMDRGHLKALQRIAP
                     PHHHAKIRLFVPDFDVPDPYYGGAAGFEKVLDLVEERCRALLDELKLTF"
     gene            4345..>5498
                     /gene="asnS"
                     /locus_tag="VFR57_07210"
     CDS             4345..>5498
                     /gene="asnS"
                     /locus_tag="VFR57_07210"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU0259185.1"
                     /translation="MTSCADILAGRAPKDAAVVLKGWVRTRRDSKAGFSFIHLSDGSS
                     FHPVQVVAPKDLPNYEKEVLHLTSGCAIEATGTVVPSPAKGQPFEMQASAVKVLGWVE
                     DPDSYPIQPKPHTFEFLREVAHLRPRTNVIAAVTRVRHTLAQAIHRFFHERGFFWVNT
                     PIITAADAEGAGALFRVSTLDLANIPRQGDKIDFSKDFFGRETFLTVSGQLNVEAYCL
                     ALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLADNADLAEALLKSVFSALLK
                     ERPDDMAFFDERVEKGIVAKLQGIIDSQFVRMDDGEAISILERAKEKFEFPVKWGVDL
                     QSEHERYLTEKRSEYGLQKRFRKIRVRREVGERDLRLDHPELGEVPAGVGV"
BASE COUNT         1043 a         1860 c         1759 g          836 t
ORIGIN      
        1 tggcgtcatg aaggccaagg ccaatgacat gccgcgcgcg ctccaagtcc ggaacttcga
       61 gctcgagctc gcgcgccgcg tcccggatgc attcagcgat cgtcgccgcc gaatcgatga
      121 tcgtgccgtc ccagtcgaag acgatcagcc ggtagctcac cgcagcacct cggacacgaa
      181 gcgcttcatg tcctccggca agggagactc gagcttgacc ggctggccgg tcagcggatg
      241 gcggaacccg agcttgcccg catgaagctg aagccgttgt ttccaaacct tattcaacgc
      301 gaaatcgccg tacttgtcgt cgccgacgat cgggtagccc gcgtgcgcca ggtgaacgcg
      361 gatctggtgc gtgcggccgg taagaagttc gacttccagc aggctgaact ccatcgaggc
      421 tttcagccgc cgagcgcgcg tgagcgcggc cagaccgcct tcgcgcacgc tcaccctgcg
      481 ctcgcccgag ccagtcacgt acttatgcag cgactcacgc agctccagcg tcttttctgt
      541 gacctcgcct ttgacgaggg cgaagtagac tttgcggacc tcgccttcgc gcagcatgcg
      601 gtgaagctcc gtcagggcgc tgcgcttctt gcaaaggata aggaggcccg acgtgtcgcg
      661 gtcgagccgg tgcgctagct cgagagatct ggcctgcgga cgcgcggcgc gcatcgattc
      721 gatgacaccg aagctgacgc cactgccgcc gtggaccgcc aggccggagg gcttgtcgat
      781 caccaagagc gcagagtcct cgaacaccac cggcagatcg agcggtctcg gcagcgcggg
      841 cttgcgctcg gcggtacgta ccggcgggat gcgcacccga tcgccgggct gcagccggta
      901 atcgggcttg acgcggccgc tattcacgcg cacctcgccg ctccgaagca cgcggtagac
      961 gtggcttttc ggaacacctt tcagcgcgcg cagcagaaaa ttgtcgatgc gctgcgcctc
     1021 ggcttcctcg cccacctcga tggtggtcgc ccgcgccttg cgtaagtcat tcatttgcca
     1081 gtatgatcag cggtcgcttc gagtcgcgag gcacaggatg agccagcggt gctggcacct
     1141 gcaacccgcc tcctgcccgg ccacagccag gcctgaagcg ctggttaatt tcgctcaccg
     1201 gaagtagcga tactactagc agcgcgcgta gggactttct cccagaaccg gcgaaacgcc
     1261 ggaattctct caaggagtat caatccgcta gcgaggcgtc tcctgcgcgc gcaggagaac
     1321 aaaaagtgaa gcgcatgttg ttcaacgcga cgcagcagga agagctgcgc gtcgcgatcg
     1381 tcgacggaca gaagctcgtc gacctcgaca tagagtccgc atcaagggag caacgcaaga
     1441 gcaacatcta caaaggcatc atcacccgca tcgagcccag cctcgaggcc gctttcgtcg
     1501 actatggcga ggagcggcac gggttcctcc cgttcaagga agtctcccgc agttatttca
     1561 aagaaggcgt cgacgtcggc cgcgcccgta tccaggatgc gctgcgcgag ggccaggaga
     1621 tcatcatcca ggtcgagaag gacgagcgcg gcaccaaagg cgccgccctc accaccttca
     1681 tctcgctggc gggacgctac ctcgtcctca tgcccaacaa cccgcgcggc ggcggagtgt
     1741 cgcgccgcgt cgagggcgag gaccgcaacg agttgcgcga cacgctcgac cagctccagc
     1801 tgcccgacgg catgagcgtg atcgcgcgca ccgcagcgat cggccggtcg ctcgaggagc
     1861 tcaactggga cctgaactac ctgctccaac tttgggctgc ggtcgaaagt gcctctcagg
     1921 accagtcgaa gaagccgttc ctcatctatc tcgagtcgag cctcgtcatc cgggcgatcc
     1981 gggactactt ccagccggac atcggcgaga tcctcatcga caccgaggag atctacgagc
     2041 aggcgaagtc gttcatgcag acggtcatgc cgggcaacgt cggcaaagtg aagctctacc
     2101 gcgatgacat cccgctcttt tcgcgcttcc agatcgagca tcagatcgag agcgcctact
     2161 cgcgtcaggt ggccctgccc tccggcggcg cgatcgtcat cgaccatacc gaggcgctcg
     2221 tggcgatcga tgtcaactcg gcgcgcgcca cgcgcggcgg cgacatcgag gagaccgcgt
     2281 tccgcacgaa cctcgaagcc gccgaggaag tcgctcggca actgcgcctg cgggacctgg
     2341 gtggtctcat cgtcatcgac ttcatcgaca tggagtcgca gcgaaaccag cgcgaagtcg
     2401 agaaccggct gcgcgactcc ctgcgctacg accgggcccg ggtgcagatc ggcaagatct
     2461 cgcgcttcgg cctcatggag ctctcgcgcc agcgcctgca ggcctcgctt gaagaaaccg
     2521 cccacatcag ctgcccgcgc tgcagcggca ccggcttcat ccgcggcacc gaatccaccg
     2581 cgctgcacgt gctgcgcatc atccaggaag aggcgatgaa ggaaaacacc ggcgcggtgc
     2641 acgcgcaggt acccgttgac gtctcctcct tcctcctgaa cgaaaagcgc gccgagatcc
     2701 agaagctcga ggcgcgattg aaggtgaaca tcgtgctcgt gcccaatccg cacctcgaga
     2761 cgccgcatta caaggtgcag cgtctgaagc acgacgagtt gaaccagatg gagcacgtgc
     2821 cggcgagcta cgagctcgtc gagcagcccg aggagcccaa ggcaccgggc gccgacgagg
     2881 agacgaagcg cgagcgccag gaagccgtgg tgaagggcat aacgcccgcg cagcccgcgc
     2941 cgatggtcga ccattcggcg ccgcgtccgc cacgcatgcc cgagccgcaa cccgtgccgc
     3001 aagcggttgc gcccgcgccg gtgctcgagg gcggcttcct ctccaagatc atcagcctgt
     3061 tcaagcccgt ggccgccccc gcagcgcagc cgcaacccgc ggcgcagccg gcgccgcagc
     3121 gcgcgcagga gccgaggcgc gaccatcagc agcggcgcga tcaccagcag cgccgtgacg
     3181 ggcgcgaccg cggacgcgat ggccggccgc gcgaggagaa ccgcgaccgc cagcagcagg
     3241 gccgtctgga gcaacgccgc ggcaacggtc agccgcgcgg cgagcgtccg aaccgcgggg
     3301 aacgcggaga gcgcggcgag cgccgacccg accagcagcg tccgccgcgt ccgcctcatc
     3361 agcagccgca gcaagcgcag ccgcagtcgc agccacagcc ccagcaacaa tcggcgccgc
     3421 gaccgccgga agcccgcaac ggcgacggcg aaccccgccg cggcggccgc cgcaaccgcg
     3481 gccgcgaccg gcagcgcgag cgtcatgacg gacggccggc acaaggcgag cgagcgccga
     3541 acggcgcgcc ttccttcacg ctgaaggaag accagctacg cgaggaccag cgccgcgagg
     3601 cgctcgcgcg caaggaggaa ctagcggccg aggagacgct gccaccgcct gcgctggcgc
     3661 cggcgccgcg catggaagaa ccgaagcgcg agccgcgccc cgaaccgaaa atcgaccccc
     3721 ggccgatcct cgagagcgag ggcctggtga tgatcgagac cgaccgcgcg aaggccgcct
     3781 acgtgccgat cgaagatcag ccgcagcagc tcggccgtcc gcgccgcgat cgtccgaagc
     3841 tcgcgccgca ggaagagcaa ctcgagcagg tcgaaactag aaagtaagct tcaattcgtc
     3901 gaggagcgcg cggcagcgct cctcgacgag gtcgaggact ttctcgaagc ctgcagcgcc
     3961 tccgtagtaa gggtccggaa cgtcgaagtc cggcacgaac agccggatct tcgcgtggtg
     4021 gtggggcggc gcgatgcgct gcagcgcctt gaggtggccg cggtccatcg ccaggatcat
     4081 gtcgaactcc tcgaagtcct gcgcgcgcac ctgccgcgcg cgctgcgcgc tcaggtcgta
     4141 accgcggctt ttggcgtggc gcagcgcgcg ctcgtccggc ggatcaccga tatgccagtc
     4201 gccgactccg gccgactcga tgcgaagctc gagcgatcgc tcgctcgcga ggtggcgcaa
     4261 cacgccctca gcggtcggag aacggcagat gtttccggtg cagacgaata agatggagcg
     4321 gcgttctttc ataagattgg gattatgact tcctgtgcag acatcctcgc cggccgtgcg
     4381 ccgaaagacg cggccgtggt cctcaagggg tgggtgcgca cgcggcgcga ttccaaggcc
     4441 gggttctcct tcatccatct ttccgacggc tcttcgttcc atcctgtcca ggtggtcgcc
     4501 cctaaggact tgccgaacta cgaaaaggag gtcctgcatc tcacctcggg ctgcgcgatc
     4561 gaggcgacgg gcaccgtcgt gccctccccg gccaaaggtc agccgttcga gatgcaggcg
     4621 agcgcggtaa aagtgctcgg ctgggtggag gacccggatt cctatccgat ccagccgaag
     4681 ccccatacgt tcgagttcct gcgcgaggtg gcgcacctgc ggccgcgcac caacgtgatt
     4741 gccgcagtca cgcgcgtgcg ccacacgctc gcacaggcga tccatcgttt cttccacgag
     4801 cgcggcttct tttgggtgaa cacgcccatc atcaccgcgg cggacgccga aggcgcaggc
     4861 gccctcttcc gcgtctccac gctcgatctt gcgaacattc cgcgccaggg cgacaagatc
     4921 gacttctcca aggatttctt cggacgcgag accttcctga cggtttcggg acagctcaac
     4981 gtcgaggcct actgcctcgc gctttccaag gtctacacgt tcgggccgac gttccgcgcc
     5041 gagaactcga acacgagccg gcacctcgcc gagttctgga tgatcgagcc cgagatcgcg
     5101 ttcgccgatc tcgcggacaa cgcggatctt gccgaggcgc tgctgaagtc cgtgttttcg
     5161 gcgctcctca aggaaagacc cgacgacatg gcgttcttcg acgagcgcgt cgagaagggc
     5221 attgtggcga agctgcaggg catcatcgat tcgcagttcg tgcgcatgga cgacggcgag
     5281 gcgatctcga tcctcgagcg cgcgaaggaa aaattcgagt tcccggtgaa atggggcgtg
     5341 gatctccagt ccgagcacga gcgctatctc accgagaagc gatcggaata tggacttcaa
     5401 aagcgcttcc gcaagatccg cgtccgccgt gaggtcggcg aacgcgatct ccggctcgat
     5461 catccagaac tcggcgaggt gccggctggt gttggagt
//
LOCUS       DASTPD010000218         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_110871, whole genome shotgun sequence.
ACCESSION   DASTPD010000218 DASTPD010000000
VERSION     DASTPD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110871"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1189)
                     /gene="hppD"
                     /locus_tag="VFR57_07215"
     CDS             complement(101..1189)
                     /gene="hppD"
                     /locus_tag="VFR57_07215"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU0259186.1"
                     /translation="MAEAFDISLNPVQTDGFEFVEYAAPDPAALGALFERMGFRAVAR
                     HRHKNVQLYKQGDVNFIVNAEPRSFAQNFARRHGPSVCAIAFRVRDAASAYEKLIERG
                     AWGVEARTGPGELNIPAIKGIGDSLIYLVDRYPENDYGNTIYDVDFKPLPGFEDFWRG
                     QAPAPAGAGLGFVDHLTHNVHRGRMQEWAEFYERLFNFREIRYFDIEGKQTGLKSKAM
                     TSPCGKIRIPINESSDDHSQIQEYLEQYQGEGIQHIALHTVDIYKTVDALKVEFLDTP
                     DTYYELLDSRLPNHGEPVEELKKRRILLDGSPSGGLLLQIFTKTMIGPIFFEIIQRKG
                     DEGFGEGNFKALFESMELDQVRRGVLTA"
     gene            complement(1218..1943)
                     /locus_tag="VFR57_07220"
     CDS             complement(1218..1943)
                     /locus_tag="VFR57_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0259187.1"
                     /translation="MPRQQRYFVPGVAAHIVQRGHNRAACFRRDNDYLLYLLHLRELS
                     ERHGCKVHAYCLMTNHVHLFLTPSTTHACIALMRDLGQRYVQHFNRHYGRRGTLWEGR
                     YGAFLAESARYVIACYRYIELNPVAAGMVADPAAYPWSSYGANAGTRVDPLIKPHAEF
                     LALASGAAERHSSYARLIAEKLDAQLVRQVEEALDSGYPLAGEGFKADLAARLGRKME
                     PGRPGRPEKKKRDESPGLPEIGL"
     gene            complement(2002..3195)
                     /locus_tag="VFR57_07225"
     CDS             complement(2002..3195)
                     /locus_tag="VFR57_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0259188.1"
                     /translation="MDIRGFRLTEAPEDFIDDPYPYYAAFREHSPVHELESGSYFISR
                     YEDVVATYRHAGASSDKKVEFKPKFGDSPLYEHHTTSLVFNDPPLHTRVRGLIMGALN
                     QRAIARMEAGVVELVEKLTQEFPAGPFEFIEAFAARIPVEVIGNLLDIPRDERAPLRA
                     WSIAILSGLEPRPTLGMLERGNRAVEDFKDYLSRLVEARRKRPGDCESDVLTRLIQGE
                     KGGARLSALELYHNCIFLLNAGHETTTNLIGNGTWLLLTNEDQLSRLKSEPALIPFAI
                     EEMLRYEGPIQLNNRRLTEKMALSGRELEAGSFITLGIGAANRDPRQFPHAERFDVAR
                     KPNRHVAFGHGEHACAGMNVARMEARIAFSRLLARFPDLELAGKPERDRRIRFRGFRK
                     LPVRT"
     gene            3215..3748
                     /locus_tag="VFR57_07230"
     CDS             3215..3748
                     /locus_tag="VFR57_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0259189.1"
                     /translation="MPRVPLADFEPQLKRRLEELWGTPPNLYKALANHPRLVAAWTEF
                     SKTLRHDTRTPRALRELVILRGAQLMRSEYEWAQHLAMARKAGVPEAKIRTLSAWRTS
                     SEFDAREKAALALGEAVTVGEVSEGVYAEAMRHFDHHDYVEIAIVAAFYAMVGRMLEA
                     MGVELEPEMRHYEPRIP"
BASE COUNT          669 a         1192 c         1289 g          706 t
ORIGIN      
        1 ccgtgcacga tcaaaatcgg ggtcagagcc cgatttccca ggcggcgata gtggatcgag
       61 agatcgcccg agcgcgcgcg gccagtttcg tggggaatgc tcatgccgtg agaaccccgc
      121 ggcggacctg gtcgagctcc atcgattcga acagcgcctt gaagtttccc tcgccgaagc
      181 cttcatcgcc cttcctctgg atgatctcga agaagatcgg gccgatcatg gtcttggtga
      241 agatctgcag cagcagcccg ccggagggcg agccgtcgag gaggatcctg cgcttcttga
      301 gctcttccac cggctcgccg tgattcggca gtctcgaatc aaggagctcg taataggtgt
      361 ccggggtgtc gaggaactcg actttcaacg catccacggt cttgtagatg tccacggtgt
      421 gcagcgcgat gtgctggatg ccctcgcctt ggtactgctc gaggtactcc tggatctgcg
      481 agtggtcgtc ggaagactcg ttgatcggga tgcggatctt gccgcaggga ctggtcatcg
      541 ccttcgattt gaggcccgtc tgcttgcctt cgatgtcgaa gtagcggatc tcgcggaagt
      601 tgaagagccg ctcgtagaac tcggcccact cctgcatgcg gccgcgatgg acgttgtgtg
      661 tgaggtggtc gacgaacccg agccccgcgc cggcgggcgc cggcgcctgc ccgcgccaga
      721 agtcctcgaa gccggggagc ggcttgaagt cgacgtcgta gatcgtgttg ccgtagtcgt
      781 tttcgggata gcggtcgacg agatagatga gcgagtcgcc gatgcccttg atcgccggga
      841 tgttgagctc gcccgggccg gtgcgagcct cgacgcccca ggcgcctcgc tcaatcagct
      901 tctcgtacgc gctcgccgcg tcgcgcaccc ggaacgcgat tgcgcacacg ctcggtccgt
      961 gccgccgcgc gaagttctgc gcgaacgagc gtggctcggc gttcacgatg aagttgacgt
     1021 cgccctgctt gtagagctgc acgttcttgt ggcgatggcg cgccaccgcc cggaagccca
     1081 tgcgctcgaa cagcgccccg agcgccgccg ggtccggcgc cgcgtactcg acaaactcga
     1141 agccgtcggt ctgtaccgga ttgagggaga tgtcaaacgc ctcagccatg accgatttta
     1201 gaccccgaaa atcggggtca gagcccgatt tccggcaaac caggagactc gtcgcgcttt
     1261 ttcttctccg ggcggcccgg ccgcccgggc tccatctttc ggccaagccg tgccgccaga
     1321 tcagctttga aaccctcgcc tgcaagcggg tagccgctat cgagcgcctc ttcgacctgc
     1381 cggacgagct gcgcatccag tttttcggcg atcaagcggg cgtagctcga atgccgctct
     1441 gcagcgccgc tcgcgagggc gagaaactcg gcatgcggct ttatcaacgg gtccacacgc
     1501 gtgccggcat tagcaccgta gctcgaccat ggatacgcgg ccgggtcagc caccatgccc
     1561 gcagccacag gattcagctc gatgtagcgg taacacgcaa tcacgtagcg cgccgactcg
     1621 gcgagaaagg cgccgtagcg cccctcccac agtgtgcctc tgcgtccgta atggcggttg
     1681 aagtgctgaa catagcgctg tccgagatcg cgcatgagag cgatgcacgc gtgcgtggtg
     1741 gacggggtga ggaagaggtg cacgtgattc gtcatcaggc aataggcgtg caccttgcac
     1801 ccatgtcgct cggacagctc gcgcaggtga agaaggtaca gcaggtagtc gttatcgcgg
     1861 cgaaagcacg ccgcacggtt gtggccgcgc tgcacgatgt gagcggcgac gccgggcacg
     1921 aagtagcgct gctgcctggg catggaagag aaacggcatc gctcgctcgc ggttgactta
     1981 atcgggctct gaccccgatt tttaggttcg taccggcagc tttcgaaagc cgcggaagcg
     2041 gatgcggcgg tcgcgttcgg gttttcccgc aagctccaag tccgggaagc gggcgagcag
     2101 gcgggagaac gcgatgcgcg cttccatgcg cgcgacgttc atgccggcgc aggcgtgctc
     2161 gccgtggccg aaggcgacgt ggcggttggg cttgcgcgcg acgtcgaagc gctcggcatg
     2221 cgggaattgg cgcgggtcgc ggttcgccgc gccgatgccc aaggtgatga aagagcccgc
     2281 ctcgagctcc ctccccgaga gagccatctt ctcggtgagc ctgcgattgt tgagctggat
     2341 gggaccttcg tagcgaagca tctcctcgat cgcgaaagga atcagggccg gctcgctttt
     2401 cagccgggaa agttgatcct cgttcgtgag aagaagccag gtcccgttgc cgatgaggtt
     2461 ggtcgtggtc tcgtggccgg cgttcaagag gaagatgcag ttatggtaga gctcgagcgc
     2521 agacaaccgc gcgccgcctt tctcgccctg gatgagccgc gtgaggacat cgctctcgca
     2581 gtcgccgggc cgcttgcgcc gcgcttccac caggcgggag agatagtcct tgaagtcctc
     2641 caccgcgcga ttgccgcgct cgagcatccc gagcgtcggc cggggctcca ggcccgaaag
     2701 gatcgcgatc gaccaggcgc gcagcggcgc gcgctcatcg cgcgggatgt cgagcaggtt
     2761 gccgatcacc tcgaccggga tgcgggcggc aaaggcttcg atgaattcaa aaggtccggc
     2821 aggaaattcc tgagtgagct tttccaccag ttcgacaaca cccgcttcca tccgcgcgat
     2881 cgcgcgctgg ttgagcgcgc ccatgatgag gccgcgcacg cgcgtgtgca gcggcggatc
     2941 gttgaacacc aggctcgtcg tgtggtgctc gtagagcggc gagtcgccga acttgggctt
     3001 gaactcgacc ttcttgtccg agctggcgcc ggcatggcgg taagtcgcca ccacatcctc
     3061 gtagcgcgag atgaagtagc ttccgctttc cagctcgtgc accggagagt gctcgcggaa
     3121 cgcggcgtag tacggatacg ggtcgtcgat gaagtcctcc ggcgcttcgg tgagccggaa
     3181 gccgcggata tccattaagt tattatccgc ccccatgcca cgcgtgccgc tggcggactt
     3241 cgagccgcag ctgaagcggc gtctcgaaga gctctggggc acgccgccca atctctacaa
     3301 ggcgctcgcc aaccatccgc gcctcgtcgc cgcgtggacc gagttctcga agacgctgcg
     3361 ccacgatacg cgcacgccgc gcgcgctgcg cgagctcgtg atcctgcgcg gcgcccagct
     3421 catgcgctcg gagtacgagt gggcgcagca cctcgcgatg gcgcgcaagg cgggcgtgcc
     3481 tgaggcgaag attcgaaccc tctctgcctg gagaacatct tcggaattcg atgcgcggga
     3541 gaaagccgcg ctcgcgctcg gcgaggcggt gacggtgggc gaggtgagcg agggggtgta
     3601 cgcggaagcg atgcggcact tcgaccacca cgactatgtc gagatcgcga tcgtggcggc
     3661 gttctacgcc atggtggggc gcatgctcga ggcgatgggc gtcgagctcg agcccgagat
     3721 gcgccattac gaacccagaa ttccatgaag acctaccgct actacgagtt cgtgatggcg
     3781 gcgttcgtca cgatcctcat ctgctcgaat ctgatcgggc cggcgaagat cgtgcagctc
     3841 gactggctgc cgttcg
//
LOCUS       DASTPD010000219         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_317103, whole genome shotgun sequence.
ACCESSION   DASTPD010000219 DASTPD010000000
VERSION     DASTPD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317103"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VFR57_07235"
     CDS             <1..98
                     /locus_tag="VFR57_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0259190.1"
                     /translation="RPKHVEFRSDLPKTNVGKILRRALREEKKAA"
     gene            173..1075
                     /locus_tag="VFR57_07240"
     CDS             173..1075
                     /locus_tag="VFR57_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0259191.1"
                     /translation="MVRGLSWMALAIACWTPLFSVAKRTLPVLDAYALGTVRYALGIA
                     LFVLLLAAFEGRRSLSFGDRFVAAVLCGMVGITGFNLFVWLGLGYTRPEHASIILALQ
                     SPLTALAVWLVHGQRPARFTLACVAAAIAGVALVITKGDPAGALAGGSLLGDGLVFLG
                     AVSWVTYTLSASRFPGWSPLRFTVMTCIPGAVGLVIANVIALAIGAAELPSIAQLSSV
                     AWQILYFSVCTVVLGVLGFNAGARALGPLNAMLMLNLIPLGVFAIEAALGRSFAAIEL
                     AGAAVVIGALVANNLYLRGVSTRR"
     gene            complement(1045..3354)
                     /locus_tag="VFR57_07245"
     CDS             complement(1045..3354)
                     /locus_tag="VFR57_07245"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0259192.1"
                     /translation="MLVFAAAAAGCYVYLRQSLPLARGEIALAGLGAPVEVLRDRHGI
                     PHIYAASLEDAHFALGFVHAQDRLWQMEMSRRLGSGWLAEILGPNALEADRFMRTLGL
                     RRVAEVNLVHYDAETRRLLDAYASGVNAYVEQRQVLPPEFWLLGVTPEPWTPIDSIVW
                     TKVMAWDLSGNWRSELLRMRLSRTLTTGRIQELLPPYPGDSPPRLPDLRAFYSAIEKK
                     PGQTPVFSLGSDPDLLLGSDPDFLGGSNSWAVSGARSASGKPLLAADPHLGLTAPPVW
                     YFAHLHAPGIDAIGGTLPGVPGILVGRNDRIAWGLTATYADVQDLYLERLDSAFATHE
                     EIIKVKGAPDERITVRRSRHGPVISDVSRNALDAAPRGYALALAWTALAEDDVTMQAA
                     LRLARAGDWNEFLAAARALHAPPQTAAYADAAGNVGFVVAGRIPVRKPENDLRGLAPT
                     PGWDARYEWRGYIAFDDLPRAFNPPGGAVVLANHKTVPPDYPHHITYEWQPPYRARRI
                     EELLAQNEKHDIASFKAMQADVVSLAARQLLPHFLSAYPEKSLQSWDGTMAAERAEPL
                     IMAAWWREFARALYADELGEAFRPNWSARAVFLENVLASERHWCDDVRTPSVETCAEL
                     LASSFEKAMQDLRTRYGEEWKWGEAHVARHRHRPFTRQPWLARIFDIRVPSGGDAYTV
                     NAGASDFNDEAEPYASRHAASMRAITNLADPQASVFIHSGGQSGNPLSPHYRDFAAAW
                     ARGEYVPMITERRRLEAGGVQRLVLTPRK"
     gene            complement(3526..4806)
                     /locus_tag="VFR57_07250"
     CDS             complement(3526..4806)
                     /locus_tag="VFR57_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU0259193.1"
                     /translation="MTTVAIVGLGYVGLPLAAEFAKKYRTIGFDLSEEKVAAYRNHVD
                     PTGEVSSAGFEAAAKLEVTTNARALREADFIIVAVPTPVDEAHNPDFGPLLGASASVG
                     QNLTRGATVVFESTVYPGATEEVCIPVIEKHSGMRWKKDFFVGYSPERINPGDKEHTL
                     TRIVKVVAGDTPQTLDRVAKVYGSVVGAGVHRASSIKVAEAAKVIENTQRDVNIALVN
                     ELAMIFHQIGIDTAEVLQAASTKWNFLPFRPGLVGGHCIGVDPYYLTYKAETLGYHPQ
                     VILSGRKINDGMGKYVAEQTVKRMILRGFSINKENIIVLGLTFKENVPDTRNSRVVDV
                     VRELESFGAKVFVHDPVACPKEAEHEYGIRLVDWEALPQAAAVVAAVAHDEFKNRPID
                     AILAKLAPNGVYVDVKNQADAAALRARGIDVWRL"
     gene            complement(4954..>5244)
                     /locus_tag="VFR57_07255"
     CDS             complement(4954..>5244)
                     /locus_tag="VFR57_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259194.1"
                     /translation="ESGHHCIGLLADHAAKALRMSDRSESGFEAAEVRRVAATKGTGR
                     VELRFSNAHEKQLSVTLPMDVAIELARLIVDLAEGTPFLKGKRPAEKRKTKP"
BASE COUNT          818 a         1687 c         1755 g          984 t
ORIGIN      
        1 agcggccgaa gcacgtcgag ttccgctccg atctcccgaa gaccaacgtc ggcaagatcc
       61 tgcgccgggc gctgcgcgag gagaaaaagg ctgcgtagaa aaatcggggt cagagcccga
      121 tttccggtac tcggctcctg ctaggggaaa tcgggctctg accccgattt ttctggttcg
      181 cggcctcagc tggatggcgc tcgccatcgc gtgctggacg ccgcttttct cggtggcgaa
      241 gcgcacgctc ccggtgctcg acgcgtacgc gctcggcacg gtgcgctacg cgctcggcat
      301 cgcgcttttc gtgctgctgc tcgcggcttt cgaaggccgg cgttcgctca gcttcggcga
      361 tcgcttcgtc gccgcggtgc tttgcggcat ggtcggaatc acggggttca accttttcgt
      421 gtggctgggg ctgggctata cacgcccgga gcacgcctcg atcatcctcg cgctgcagtc
      481 gccgctcacc gcgctcgcgg tctggctcgt gcacgggcag cgcccggccc ggttcacgct
      541 tgcctgcgtc gccgcggcga ttgccggagt ggcgctggtg atcacgaaag gcgatccggc
      601 gggcgcgctc gccggcggaa gcctcctggg cgacggcctt gtcttcctcg gcgcggtctc
      661 ctgggtgact tacacgctct cggcgagccg ttttcccggc tggtcgccgc tgcgcttcac
      721 ggtgatgacc tgcattcccg gcgcggtcgg actcgtcatt gcgaacgtga tcgcgcttgc
      781 gatcggagcc gcggagttgc cttcaatcgc acagctctcg agcgtcgcgt ggcagatcct
      841 gtatttctcg gtctgcacgg tggtgctcgg cgtgctgggg ttcaacgctg gcgcgcgcgc
      901 gcttggcccg ctgaacgcga tgctgatgct caacctcatc cccctgggcg tatttgcgat
      961 cgaagcggcg ctcggccgca gcttcgccgc gatcgagctc gcgggcgcgg ccgtcgtgat
     1021 cggcgcgctg gtagcgaaca acctctactt gcggggtgtg agcaccaggc gctgaactcc
     1081 gccggcctcg aggcgccggc gctcggtaat catgggcacg tactcgccgc gcgcccaggc
     1141 ggcggcgaag tcgcggtagt gcggcgagag cgggttgccc gactggccac ccgagtggat
     1201 gaacaccgag gcctgtgggt cggcgaggtt cgtgatcgcg cgcatgctcg cggcatgacg
     1261 gctggcgtag ggctccgctt cgtcgttgaa gtcgctcgcg cccgcgttca cggtgtacgc
     1321 atcgccgccg ctcggcacgc ggatatcgaa gatgcgcgcg agccatggct ggcgggtgaa
     1381 cgggcgatga cggtgccgcg caacgtgcgc ctcgccccat ttccactcct cgccgtagcg
     1441 cgttctcaga tcctgcatgg ctttttcgaa agacgaggcg agaagctctg cgcatgtctc
     1501 cacggaaggg gttcgaacgt cgtcgcacca atgccgctcg cttgcgagga cgttttcgag
     1561 gaacacggcg cgcgcgctcc agttcggacg gaacgcttcg ccgagctcgt ccgcgtacag
     1621 cgcgcgcgcg aattcgcgcc accaggcggc catgatgagc ggctccgcgc gctcggccgc
     1681 catggttccg tcccaggatt ggagactctt ctccggataa gcagaaagga aatgagggag
     1741 caattgccgt gctgcgagcg acacgacgtc ggcttgcatg gctttgaaac tcgcgatgtc
     1801 gtgcttttca ttttgcgcaa gcaactcctc gatgcgccga gcacggtagg gcggctgcca
     1861 ttcgtaggtg atgtggtgcg gataatcggg cggcacggtc ttgtggttcg cgagtaccac
     1921 ggcgccgccc ggcggattga atgcgcgcgg caggtcgtcg aaggcgatgt agccgcgcca
     1981 ttcgtagcgc gcgtcccagc cgggcgtggg cgcgaggccg cgcaggtcgt tttcgggctt
     2041 gcgcaccgga atgcgcccag cgacgacgaa gccgacgttg cccgccgcgt cggcataggc
     2101 tgcggtctgc ggcggtgcat gcagggcgcg tgcggcggcg aggaactcgt tccagtcgcc
     2161 cgcgcgggca aggcgcagcg ccgcctgcat ggtgacgtcg tcctcggcga gcgcggtcca
     2221 ggcgagcgcg agcgcatagc cgcgcggcgc ggcatcgagc gcgttgcgtg aaacgtcgga
     2281 gatgacggga ccgtggcgcg aacggcgcac ggtaatgcgc tcgtccgggg cgcccttcac
     2341 cttgatgatc tcctcgtgcg tggcgaacgc gctgtcgagc ctctcgagat agaggtcctg
     2401 tacgtcggcg taggtggcag taaggcccca cgcgatgcgg tcgttgcgcc cgacgagaat
     2461 ccccggcact ccggggagcg tgccgccgat cgcgtcgatc cccggcgcgt gcaggtgcgc
     2521 gaagtaccat accggcggcg cggtaaggcc caggtgagga tccgctgcca gcagcggctt
     2581 cccgctcgca ctccgcgctc ccgacaccgc ccagctattg gaccctccca gaaaatcggg
     2641 gtcagacccc aatagcaaat cggggtcaga ccccaacgag aacaccgggg tctgacccgg
     2701 cttcttttcg atcgctgaat agaaggctcg caggtccgga aggcgcggcg gtgaatcgcc
     2761 gggatagggc ggcaggagct cttggatgcg cccggtcgtc agcgtgcgcg agagccgcat
     2821 acgcaatagt tcggagcgcc agttcccgct gaggtcccag gccatcacct tcgtccagac
     2881 gatcgagtcg atcggggtcc acggctccgg ggtgacgccg agcagccaga actctggcgg
     2941 caatacctgc ctttgctcca cgtaagcgtt tacccctgat gcataagcgt ccagcagccg
     3001 gcgggtttca gcgtcgtagt gaacgaggtt cacctccgcc acccgtcgca gccccagtgt
     3061 ccgcatgaat cggtcggctt ccaaggcgtt cggccccagt atctcggcga gccaccccga
     3121 tcccaggcgc cgactcatct ccatctgcca gagccggtcc tgggcgtgga cgaagccgag
     3181 cgcgaagtgc gcgtcctcga gcgacgctgc gtagatgtgg gggatgccgt ggcggtcgcg
     3241 caacacctcc actggcgccc cgagcccggc gagggcgatc tcgccgcgcg cgagcggcag
     3301 cgattggcgc aagtagacgt agcagccggc tgcggcagca gcgaaaacga gcaaaaggag
     3361 ccctagaacc cgcagcgcac gccgcatttg cccgagaata acagcgtgat ccagctcgta
     3421 tgccggcaat cctgctaccg gggtctaacc ccgggcttac tgcgcttgcg gtctagctcg
     3481 tgcgcgggca gcgtgcgctg agcttgctgt cggcgatgcg ctgcctcaaa gacgccatac
     3541 gtcgatgccg cgcgcgcgaa gcgctgcggc gtcggcttgg ttcttcacgt ccacatagac
     3601 gccgttcggt gcaagcttcg ccaagatcgc gtctatgggg cgattcttga actcgtcgtg
     3661 cgccacggcc gccacgacgg ccgcggcctg tggcagcgcc tcccagtcca caagacggat
     3721 tccgtactcg tgctccgctt ccttgggaca ggcgaccgga tcgtgcacga atactttcgc
     3781 gccgaaggac tccagctcgc gcaccacgtc cacgacgcgc gagttgcgcg tgtcggggac
     3841 gttttccttg aacgtaagcc cgagcacgat gatgttttcc ttgttgatgg agaaacccct
     3901 gaggatcatg cgcttcacgg tctgctcggc gacgtacttc cccatgccgt cattgatctt
     3961 gcggcccgac aggatcacct ggggatggta gccgagggtc tcggccttgt acgtcaggta
     4021 gtaggggtcc acgccgatgc agtggccgcc gacgaggccg ggccggaagg gcaggaagtt
     4081 ccacttggtg ctcgccgctt gcaggacctc ggcggtgtcg atgccgatct ggtggaagat
     4141 catcgcgagc tcgttcacca gggcaatgtt gacgtcgcgc tgcgtgttct cgatgacttt
     4201 ggcggcttcg gcgaccttga tggagctcgc gcggtgcacg cctgcgccga ccacgctgcc
     4261 atagaccttc gcgactcgat ccaacgtctg cggggtgtca cccgccacga ctttgacgat
     4321 cctcgtgagg gtgtgctctt tgtcgcccgg gttgatgcgc tcgggcgagt agccgacgaa
     4381 gaagtctttc ttccacctca tcccggagtg cttctcgatg accgggatgc agacttcctc
     4441 ggtcgcgccg ggatagacgg tggattcgaa caccacggtt gcacctcggg tgaggttttg
     4501 cccgacagag gcgctcgcgc cgagcaacgg gccgaaatcg gggttgtgcg cctcgtcgac
     4561 cggcgttggg acagcaacga tgatgaaatc cgcctcgcgt agcgcgcgtg cgttggtggt
     4621 gacctccagc ttggcggcgg cttcgaatcc cgcgctgctg acttcgcccg tggggtcgac
     4681 atggttcctg taggcagcga ccttctcctc cgaaaggtcg aagccgatgg tgcggtattt
     4741 cttggcgaac tccgcggcga gcggcagccc gacgtaaccc aggcccacaa ttgcgacggt
     4801 ggtcatcgtt atagtccagc gagtaacgcc gcggcttcct tccgcgcgga atggcggctg
     4861 tcgatcctgg ccactatatc gcgctgggct agataggcaa ccgcatggct tttggcgtta
     4921 cgagccgtat gcggcgcgtc cggactttcg cttctaaggc ttggtcttgc gcttctccgc
     4981 ggggcgcttg cctttcagga aaggagtgcc ttcggccaag tcaacgatca agcgggcgag
     5041 ctcgatggca acgtccattg gtaatgtgac ggacagctgc ttttcgtgcg cgttggaaaa
     5101 ccgcagctca actcgtcccg ttcctttggt ggccgcaacc cgccgcactt ccgctgcttc
     5161 gaagcccgat tccgatctgt cactcatccg caatgccttt gccgcgtgat cggcaagaag
     5221 cccaatgcaa tgatgacccg actc
//
LOCUS       DASTPD010000220         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1747024, whole genome shotgun sequence.
ACCESSION   DASTPD010000220 DASTPD010000000
VERSION     DASTPD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747024"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1291
                     /locus_tag="VFR57_07260"
     CDS             77..1291
                     /locus_tag="VFR57_07260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0259195.1"
                     /translation="MPEISRRASSLGTENAFVVLAEVNALARQGKDIVSFCIGQPDFP
                     APDNVQEAAIRAIKGGKHGYTPSAGIDELRQAAAKYMAGMRGGLPIRPEDVVVGAGAK
                     PFIAYAVVSTTDYGVGDEVIYPNPGFPIYESQIAANGAKPVPIHLHEGRNFSFDPNEL
                     EKLITKKTKLLILNYPHNPTGGCLTRKELETIAEVLKKHPQVWVYADEIYSRLVFQGE
                     FFSIAQVPGMYERTIISDGASKTWAMTGWRIGFTSNPQLAPVFTRWITNTESCASQIS
                     QWAAVEAINGPQDSASRMKQSFLERRDLIVGLLNEVPGVQCQVPGGAFYVWPNVTEAC
                     RRIGAADSEEFRKRLLHEAGVAVLADIHFGTRVPGEGQHVRFSYAASKQAIEAGVQRM
                     ADFIRKNTKKAA"
     gene            1288..1740
                     /locus_tag="VFR57_07265"
     CDS             1288..1740
                     /locus_tag="VFR57_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0259196.1"
                     /translation="MSARKLPPVTVAAVIERGGKLLLVQEVIDGRLVLNQPAGHLDPG
                     ESLIEACRREVLEETAHVFEPTGLVGIYRWHYARKDVTFLRFCFRGRIGGTEDRPLDP
                     QIRGLHWLTPQELKERSAEHRSPLVQKCVDDFLAGRDFPLDVLSTEFA"
     gene            1737..2792
                     /gene="mnmA"
                     /locus_tag="VFR57_07270"
     CDS             1737..2792
                     /gene="mnmA"
                     /locus_tag="VFR57_07270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU0259197.1"
                     /translation="MKVVVGMSGGVDSAVAALLAKRAGHDVVGLFMKNWEDDDSDEYC
                     STREDLIDAAAAADRIGIELEAVNFSAEYKERVFAEFLREYAAGRTPNPDVLCNAEIK
                     FRAFLDHAMRLGAAKIATGHYARVEQLDGAFRLLRGKDASKDQSYFLHRLTQEQLSRV
                     VFPVGDLNKSEVRRIALEAGLPNHAKKDSTGICFIGERPFREFLNRYLPRVPGPIVDD
                     EGKRIGEHIGLAFYTIGQRKGIGIGGAGEPWYVAAKVLSSNTLVVVQGHDHPLLMKKT
                     LTASDAAWVAGEPPAAPSAHTAKTRYRQADAPCTLSKVLESEIRVDFGAPQWAVTPGQ
                     SVVLYDNEVCLGGGVIT"
     gene            complement(2785..3402)
                     /locus_tag="VFR57_07275"
     CDS             complement(2785..3402)
                     /locus_tag="VFR57_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0259198.1"
                     /translation="MKLLSSTASPYTRKVRIVLAEKKIECEIQPVDVQPVENPVNAHN
                     PLGKIPTLILDDGSALYDSRVIVEFLDGKSPIGRLIPEDLRERVAVRRWEALADGVLD
                     AGLLVRYESLRDATERSHAWTDKQLARIRRGMAEMAGGLGERAWCHGERYSLADIAVG
                     CCLGWLGFRKPGDVDWHVEYPSLAKHYEKLMARPAFAETVPQVPR"
     gene            complement(3414..>3894)
                     /locus_tag="VFR57_07280"
     CDS             complement(3414..>3894)
                     /locus_tag="VFR57_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU0259199.1"
                     /translation="KTARLLIVGQAPGRLVHETGIPWNDPSGEVLRAWLKLDRAAFYD
                     TSRIAIVPTGLCYPGSVGGADLPPCRECAPLWQPRFRAALPEIGLTLLVGSYAQAYYL
                     KASRKKTLADTVRAFRDYLPAFFPLPHPSWRNLLWLKRNPWFERDVIPALRRRVKAL"
BASE COUNT          684 a         1263 c         1316 g          631 t
ORIGIN      
        1 tcgatgtccg cgagcttgcg gccgtgctcg tacgcgacgc aattgaccag catggggcgg
       61 cataatacgg gtccttatgc ctgaaatcag ccgccgggcc tcgagcctgg gaaccgaaaa
      121 cgccttcgtg gtcctcgccg aagtgaacgc gctcgcgcgc cagggcaagg acatcgtcag
      181 cttctgcatc ggccagcccg acttcccggc gccggacaac gtgcaggagg cggcgatccg
      241 cgccatcaag ggcggcaagc acggctacac cccctcggcg ggcatcgacg agctgcgcca
      301 ggcggcggcg aaatacatgg ccggcatgcg cggcggcctg ccgatccgcc ccgaggacgt
      361 ggtggtcggc gccggcgcca agcccttcat cgcctatgcg gtggtctcca ccaccgacta
      421 cggcgtcggg gacgaggtga tctaccccaa ccccggcttc ccgatctacg agtcgcagat
      481 cgccgccaac ggcgcgaagc cggtgccgat ccacctgcac gagggccgca atttctcttt
      541 cgatccgaac gagctcgaga agctgattac caagaagacc aagctgctca tcctcaacta
      601 cccgcacaac ccgaccgggg gttgcctgac ccgcaaggaa ctggagacga ttgccgaggt
      661 gctgaaaaag cacccgcagg tctgggtcta cgcggacgag atctattcgc gcctggtgtt
      721 ccagggcgaa ttcttctcca tcgcgcaggt ccccggcatg tacgagcgca ccatcatttc
      781 cgatggtgcg tcgaagacct gggcgatgac cggctggcgc atcggcttta cctccaaccc
      841 gcagctcgcg ccggtgttca cgcggtggat cacgaacacc gagtcgtgcg cgtcgcagat
      901 cagccagtgg gccgcggtgg aagcgatcaa cggcccgcag gattccgctt cgcgcatgaa
      961 gcagtccttc ctggagcggc gcgaccttat cgtcggcctg ctgaacgaag tgccgggggt
     1021 gcaatgccag gtgccgggcg gcgcgttcta tgtatggccg aacgtcaccg aggcctgccg
     1081 ccgtattggc gccgcggatt ccgaggagtt caggaagcgc ctgcttcacg aagccggcgt
     1141 cgcggtgctc gcggacatcc acttcggcac gcgcgtgccg ggcgaaggcc agcacgtgcg
     1201 cttctcgtac gccgcttcca agcaggcgat cgaggcaggt gtgcagcgca tggccgactt
     1261 catccggaag aacaccaaaa aagcggcatg agcgcgcgca agctgccccc ggtgaccgtc
     1321 gccgcggtca tcgaacgggg cggaaagcta cttcttgtgc aggaggtaat tgacggccgc
     1381 ctggtgctga accagccggc cggccatctc gatccgggcg aatcgctcat cgaggcctgc
     1441 cggcgcgagg tgctcgagga gaccgcgcac gtgttcgagc cgaccgggct ggtgggcatc
     1501 taccgctggc actacgcgcg caaggacgtc accttcctgc gcttttgctt tcgcggccgc
     1561 atcggcggca ccgaggaccg tccgctcgac ccgcagatcc gtggcctgca ctggctcacg
     1621 ccgcaggagt tgaaggagcg tagcgcggag caccgttcgc cgctcgtgca gaagtgcgtc
     1681 gacgacttcc tggccgggcg cgatttcccg ctcgacgtgc tctcgaccga gttcgcatga
     1741 aggtcgtcgt gggcatgtcg ggcggcgtcg actccgccgt ggccgcgctc ctcgcgaagc
     1801 gcgcggggca cgacgtggtc ggcctcttca tgaagaactg ggaggacgac gactcggacg
     1861 agtactgctc gacgcgcgaa gatttgatcg acgcggcggc ggcggcggac cgcatcggca
     1921 tcgaactgga ggccgtgaac ttctccgccg agtacaagga gcgcgtgttc gccgagttcc
     1981 tgcgcgagta cgccgccggg cgcacgccca acccggacgt gctctgcaac gcggagatca
     2041 agttccgcgc cttcctcgac cacgccatgc gcctgggcgc ggcgaagatc gcgaccggac
     2101 attacgcccg cgtggagcag ctcgacggcg catttcgcct gctgcgcggc aaagacgcct
     2161 cgaaggacca gagctacttc ctccatcggc tcacgcagga gcagctttcg cgcgtggtat
     2221 ttccggtcgg ggacctcaac aaaagcgagg tgcggcgcat cgcgctggag gccgggctgc
     2281 ccaaccacgc caagaaggac tcgaccggca tctgcttcat cggcgagcgc ccgttccggg
     2341 agttcctgaa ccgctatctg ccgcgcgtgc ccggtcctat cgtggacgac gaaggcaaaa
     2401 ggatcggcga gcacatcggg cttgccttct acacgatcgg ccagcgcaag ggcatcggca
     2461 tcggcggcgc gggcgagcct tggtacgtgg cagcgaaggt tttgagctcc aataccctcg
     2521 tcgtagtgca aggccacgac catcccttgc taatgaagaa gaccctgacg gcctcggacg
     2581 ccgcctgggt ggccggagag ccgccggctg ccccatcggc gcacactgcc aagacgcgct
     2641 accggcaggc cgatgcgccg tgcacgctat cgaaagtgct ggagagcgag atccgcgtgg
     2701 atttcggcgc gccgcaatgg gcggtgacgc cggggcagtc cgtggtcctc tacgacaacg
     2761 aggtctgcct gggcggcggc gtaattacct agggacttgc ggcacggttt cggcgaaagc
     2821 cgggcgtgcc atcagcttct cgtagtgctt ggcgagcgaa gggtattcga cgtgccagtc
     2881 gacgtcgccg ggcttgcgga agccgagcca gccgaggcag cagccgaccg cgatgtcggc
     2941 gagcgaatag cgctcgccgt ggcaccaggc gcgctcgcca agaccgcccg ccatttccgc
     3001 catgccgcgc cggatgcgcg cgagttgctt gtcggtccac gcgtggctgc gctcggtcgc
     3061 atcgcgcagc gattcgtagc gaaccagcag gccggcgtcg agcacgccgt cggcgagcgc
     3121 ttcccagcgc cgcaccgcga cgcgctcgcg caggtcttcc ggaatgaggc gtccgatcgg
     3181 cgatttgccg tcgaggaatt cgacgatgac gcgagaatcg tacagcgcgc tgccgtcgtc
     3241 gagaatgagc gtcgggatct tgccgagcgg gttgtgggcg ttgaccgggt tctcgaccgg
     3301 ctgcacgtcg accggctgga tctcgcattc gatcttcttc tcggcgagga cgatgcgcac
     3361 tttgcgcgta tagggactgg cggtcgaaga gaggagtttc atcggggcgg cgattataga
     3421 gccttcactc gccgccggag cgccggaatc acatcgcgtt cgaaccaggg attgcgcttg
     3481 agccagagga ggttgcgcca gctcgggtgc ggcagcggaa agaacgcggg aaggtaatcc
     3541 cggaatgcac gaaccgtgtc cgccagtgtt ttctttctgg aagcctttaa gtaataggct
     3601 tgagcgtagc tcccgacgag gagcgtaagc ccgatctcag gcagcgccgc gcgaaaacgc
     3661 ggctgccaga gcggcgcgca ttcgcggcac ggcggcaggt cggcgccgcc gacactgcct
     3721 ggatagcaaa ggccggtcgg cacgatggcg atgcgcgagg tgtcgtagaa ggctgcgcga
     3781 tcgagcttga gccacgcgcg cagcacttcg cccgaaggat cgttccacgg aatgccggtt
     3841 tcgtgcacga gacgcccggg cgcctggccg acaatcagca ggcgcgcggt tttt
//
LOCUS       DASTPD010000221         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1134935, whole genome shotgun sequence.
ACCESSION   DASTPD010000221 DASTPD010000000
VERSION     DASTPD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134935"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFR57_07285"
     CDS             <1..452
                     /locus_tag="VFR57_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259200.1"
                     /translation="KAAIELQLGDARLALEREAPRSFDVLAVDAFSSDAIPVHLITRE
                     ALGVYLRHVKHDGIVAFHVSNRFLDLIPVVARLAKEQGAHAVLVRDDPDEEEDYRRSR
                     TDWVLVSRDPAALKRPAILERGAAPPEDRPEWRTWTDDYSNLIQILK"
     gene            572..1714
                     /locus_tag="VFR57_07290"
     CDS             572..1714
                     /locus_tag="VFR57_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU0259201.1"
                     /translation="MSRRRFTPEERAALWRGWKSGLTLSEIGQGLNRRAVSVLNILRR
                     AGGFEPRTRRRAARSLKAGEREEISRGLAAGLAVREIARQLGRAACTVSREIGRNGGR
                     HGYRAADADARAWQRARRPQACLLARRPWLRNWVAERLQRDWSPRQIAIGLERTFAHD
                     GSMRVSHETIYRSLFVQARNVLKKELLGHLRRGGFMRRPRTQPGAQPTIVDGISIHQR
                     PAEVEERAVPGHWEGDLLMGGASSQIATLVERHSRYLMLVKVDRKDTGTVTQALAQKI
                     RQLPAELRRSLTWDRGSEMAAHKDFTVATDVQVYFCDPHSPWQRGSNENTNGLLRQYF
                     PKGQDVSHVTQAELNRVARLLNERPRETLNWLTPLEALRATVASTG"
     gene            complement(1800..>2148)
                     /locus_tag="VFR57_07295"
     CDS             complement(1800..>2148)
                     /locus_tag="VFR57_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU0259202.1"
                     /translation="TAKAVVAYQGKHRVRVLFAISFTILLHVVEMWLFGLVMWALLQW
                     PACGAIAGADDSPFFDMIYLSAISYTTVGFGDVAPVGPIRFLVATEAITGFVLIAWSA
                     SFTYLEMERFWKQ"
BASE COUNT          399 a          652 c          737 g          360 t
ORIGIN      
        1 gcaaggcggc gatcgaactg cagctcggcg acgcccgcct cgcgctcgag cgcgaggcgc
       61 cgcgcagctt cgacgtgctc gcggtcgatg ccttctcaag cgacgcgatc ccggtgcacc
      121 tcattacacg cgaggcgctc ggtgtgtacc tgcgccacgt caagcacgac ggcatcgtcg
      181 cctttcacgt gtcgaaccgt ttcctcgacc tcatcccggt ggtggcgcgg cttgccaagg
      241 agcagggcgc gcacgccgtg ctggtgcgcg atgaccccga cgaggaagag gattaccggc
      301 gctcgcgcac cgactgggtg ctggtgtcgc gcgatccggc cgcgctcaag cggccggcga
      361 ttttggagcg gggcgcggcc ccgcccgaag accgcccgga atggcggacc tggaccgacg
      421 attactcaaa cctgattcag atcctgaaat aggaaaaggg gtcaggaccc ttttcgcgta
      481 gctgcgcgta gctcgccgga aacagggtcc tgggtgatgt aaacccgtcg aggcaacacc
      541 tttgattcaa ttgtgggagg gagggtgttg catgtccaga agaaggttta cgcccgagga
      601 gcgggcggcg ttgtggcgtg ggtggaagag cggcttaacg cttagcgaga ttggccaagg
      661 cctgaatcgt cgggccgtta gtgttcttaa cattctgcgg cgcgccggcg ggttcgagcc
      721 gagaacccgg cggcgcgccg cgcgatcgct caaggcgggc gagcgcgaag agatctcccg
      781 gggcttggcg gcaggactag cagttcgaga gattgcgcgg cagcttggca gggcggcttg
      841 cacggtgagc cgtgagattg gccgcaatgg cggtaggcat ggctatcggg ccgccgatgc
      901 cgacgcgcgc gcctggcagc gcgctcggcg gccgcaggca tgcctgctgg cgcgacggcc
      961 gtggctcaga aactgggtgg cagagcgctt gcagcgtgac tggtcgccgc gccagatcgc
     1021 cattggcctg gagcgcacat tcgcgcatga tggctcgatg cgggtatcgc acgagacgat
     1081 ttaccgcagc ctctttgtcc aggcgcgcaa tgtgttgaag aaggagcttc tgggccacct
     1141 gaggcgtggc gggttcatgc gccggccacg tactcagcca ggagcccagc cgacgattgt
     1201 cgatggcatc tcgattcatc agcgtccggc cgaagtcgag gagcgggcgg ttccggggca
     1261 ttgggagggc gacttgctca tgggcggcgc aagctcgcag atcgccacct tggtcgagcg
     1321 ccattcccgc tatctgatgc tggtgaaggt ggatcgcaaa gacaccggca ccgtgaccca
     1381 ggccttggcc caaaagattc gccagctacc ggccgagctg cgccgctcac tgacgtggga
     1441 ccgcggcagc gaaatggcgg cgcataagga tttcaccgtg gccaccgatg ttcaagtcta
     1501 cttctgcgat ccgcacagcc cttggcagcg cggtagcaac gagaacacca acggcttgct
     1561 gcgccagtac ttccccaagg gccaggatgt gtctcacgtt acgcaggccg agctcaaccg
     1621 cgtcgcgcgt ctgcttaacg agcgcccacg ggaaacgcta aactggctta cgccgttgga
     1681 ggcattacga gcaaccgttg cctcgacggg ttgagatcac ccaggaccct tttttcggaa
     1741 aaggggtcag cagccgttcc gttgcttcgc gtagcccgca ggaagaagcg gcggatcctt
     1801 tactgcttcc agaagcgctc catctcgaga tacgtgaaag acgccgacca ggcgatgagc
     1861 acgaatccgg tgatcgcctc ggtggcgacg aggaagcgga tcggaccgac cggcgcgacg
     1921 tcgccgaagc cgaccgtggt atagctgatc gccgagaggt agatcatgtc gaagaatggc
     1981 gagtcgtccg cgccggcgat cgcgccgcag gcgggccact ggagcagcgc ccacatcacc
     2041 aggccgaaca gccacatctc gaccacgtgc agcaggatgg tgaaggagat ggcgaaaagc
     2101 actctgacac ggtgcttgcc ctggtacgcg acgaccgcct tggcggtg
//
LOCUS       DASTPD010000222         9857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_524455, whole genome shotgun sequence.
ACCESSION   DASTPD010000222 DASTPD010000000
VERSION     DASTPD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524455"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFR57_07300"
     CDS             complement(<1..735)
                     /locus_tag="VFR57_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0259203.1"
                     /translation="MNLPAYQTLLLEKRDAHVLVVTLNRPQSLNALSTQMGRDKIDLW
                     TRLTAEPGELRCVVLTGAGERAFCAGADLKERDGMTETVWRVQHEIFERAFVALMELP
                     LPVIAAVNGHAFGGGLEMALACDFIYAAKAARMALSETRLGLMPGGAGTQNLARAAGE
                     RRAKELVMTAQPFDAQQGYEWGIVNRVCDNALNDALATARTISDNAPLAVRQAKKSVH
                     YGLQMDLLTGYRFEIEAYNQLVATEDR"
     gene            753..1076
                     /locus_tag="VFR57_07305"
     CDS             753..1076
                     /locus_tag="VFR57_07305"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEU0259204.1"
                     /translation="MKVLVLVLCLLSGCAVLETKGAVVGCQAADTVTTLHAVSLGARE
                     ANPVVRWLLETFGPGGFIAAKVGVTLLFLHYYPDISSDVVILVNGVTCAVAAHNLRVA
                     SQLPR"
     gene            complement(1061..1957)
                     /locus_tag="VFR57_07310"
     CDS             complement(1061..1957)
                     /locus_tag="VFR57_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0259205.1"
                     /translation="MNVQDVDLNLLRVFDAVLHEKGVTPAAARLGLTQPAVSNALARL
                     RKLFGDPLFVRTPGGVDATPFARELAEPVRQALALLESALAHGPGFDPTSSTRAFRFY
                     MSDLGQIEFLPPLVERLHRDAPGVRLEAVALEVDDIGNALATGALDVAIGFLPGLGSP
                     VRRQALFRDPYLCLMRADHSITTLTKKRFLEASHALVTYRGGHRVIEEALDRAGLTRR
                     ITLRVPHFTVVPMVLERTDLILTLPARVARVFERRGKLKALPPPVAIPPAEVALHWHE
                     RFEADPGNRWLREAMVELYRGS"
     gene            2043..2984
                     /gene="dctP"
                     /locus_tag="VFR57_07315"
     CDS             2043..2984
                     /gene="dctP"
                     /locus_tag="VFR57_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0259206.1"
                     /translation="MRKPLLVAVAAALPLAAHAQETTLKLVSAFGETTTYVKHMTPWI
                     QKFNAEGKGIAQINFIGGPKAIPTFEVGTSVKNGVVEMAMSTGAFYTNLMPEADFLKL
                     TQITIAEQRKNGAFDYINKVWNQKANMAYLARMVDETPFHLYLTKKIDKPDLTGLKLR
                     ITPVYRDFFASMGASLIQTPPGEVYTALERGVVDGYGWPIQGIFDLNWHERTKFRVDP
                     GFYNAEVSIVMNLDTWKKLSPKQREYVQKQALALESMNDVWRKLNAEETKKQAESGIQ
                     AIKFDNAFREKAYEVAWAAATKQSPEHAPQMRKLFSK"
     gene            2981..3427
                     /locus_tag="VFR57_07320"
     CDS             2981..3427
                     /locus_tag="VFR57_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0259207.1"
                     /translation="MKALYARVLEWLAACAAVLLGASALAITIDVIGRNMGMGTLPWI
                     LEVSEYVLPLATFLIAPWLLYRNDHVRLDILALTRPWLGHVANIVGLGVCAVLVVYGV
                     RTILNSAAQGAMIFKSVVFPEWWLYLPVPLCFGLLTIEFVRRLARR"
     gene            3424..4740
                     /locus_tag="VFR57_07325"
     CDS             3424..4740
                     /locus_tag="VFR57_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0259208.1"
                     /translation="MSWWVAACILFGSLSLLFALALPAAFAFLGINIVGAWVWLGKDA
                     GLIQLVRNGVASISSFSLTPIPFFILMGEVLFHTGVAMKAIDAIDRLIWRIPGRLAVV
                     AVVAGTVFSAISGSTIATTALLGSLLLPQMLARGYHPNMAMGPIMAIGGVDMLIPPSA
                     LTVLLGSLAGISISQLLVAGIVPGVLMSCLFVGWIVLRASMDPSLAPRFEHEALTAWA
                     RLKPLLLYVAPLVLIFVVVVAAMAAGWATPTEAAAIGAVATVLVAWIYRALTWSNLMR
                     ALMGTAGISGIILFIIVGATTFAQILTFSGATNGLVAAITDAQLAPWIVLAAMLVILL
                     VLGCFIDQVSMMLICLPFFMPLVGAYSFDPIWFGVLFLICMQLGLLTPPFGMLLFTMK
                     SVAPKEIAMSQVWHAVTPYVVMGMVALALVIVFPPLATWLPKALIK"
     gene            4743..6812
                     /locus_tag="VFR57_07330"
     CDS             4743..6812
                     /locus_tag="VFR57_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEU0259209.1"
                     /translation="MERSFKEEVAKLRLGDGETFHGEGILAVTKALLQSGVAYVGGYQ
                     GAPVSHVMDVLNDARDILDELGVHVETNANEAGAAAMLGASINYPMRGAVAFKSTVGT
                     NVASDALSNLASAGVKGGALLVLGEDYGEGSSIIQERSHAFAMKSQIWLLDPRPNLPK
                     IVEMVEQGFELSEASSTPVMLELRIRACHVQGSFKTRKNQAPQFSKKAFLQNPDFDYG
                     KICLPPATYAQEKHKVDVRWPAAVHFIRSRKLNEFFDGEQKDLGIVCQGGLYNAVIRA
                     LQMLGLADAFGASRVPIYCMNVTYPLIPDEITGFCQDKKSVLIVEEGQPAYIEDAVLA
                     ALRRAGANSVKVSGKDVLPLAGEYTGEVVLTGLSRFLDRSERVQPILSLKQKASGLLA
                     GLPARPPGFCVGCPERPVFSAMKVLEKESGKFHLSADIGCHLFSTLPPFNIGNTVLGY
                     GLGLASNSAVHPMFGKRTVTMMGDGGFWHNGLTTGIANTVFNKNDGILVIMKNGYSSA
                     TGTQELPSSPQHSEHKAPDMSMERALEGIGVGWVKTVDNYRVGEVAKTLKEAMTTDYP
                     GLKVIIAEGECQLERQRKLRPLVSKQLSEGKRTVRVKYGVDEDTCTGDHSCIRLSGCP
                     TLTIKDNPDPLRRDPVATVIDGCVGCGLCGENAHAAVLCPSFYRAEVIQNPTWWDRLW
                     NASSPGK"
     gene            6785..8899
                     /locus_tag="VFR57_07335"
     CDS             6785..8899
                     /locus_tag="VFR57_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEU0259210.1"
                     /translation="MEREFTREIELLRLGDGDTFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHLLDVMVQARDYLDELGVHVEACTSEASAAAMLGASINYPARGAVTWKSIVGT
                     NVAADALSYLASAGVMGGTLIVLGEDYGEGGVVAQERSHAFALKSLMWLLDPRPNLAT
                     IAEMVEHGFELSEASNTPVMMELRIRACHVRGSFRTRANVAPSVSTRQRLDGPPREFI
                     YDRLAHPPATFRQEKLKSEVRLPAARKYILENRLNEVFPGSENLGIVVQGGLYNGLMR
                     ALRTLGLADPFGATRIPILALNVVYPLVPEEIVGFARDKTALLILEEGQPEFIEQEIA
                     TFLRRADEPAKLHGKDCMHMAGEYNVESLVRGIARFVELHARHLDVSAGGRWLDSVQK
                     GKSAASSLLGALPARPPTFCVGCPERPVFSAMKLVAKDIGRPHVAMDVGCHAFASFEP
                     FSQGNTLLGYGMSLASAAGVAPMQAKRAVAVMGDGGFWHNGLLSGVASNLMNKGDGVL
                     VIMKNGYASATGTQELISSPPEEARREALGQSAADADRTIENTLTGLGVKWLRTVNNY
                     DVATMTKTYREAMTTPDKGLKVIVAEGECQLERQRRLKPAVVERLARKERHVRVKYGV
                     DEDTCTGDHSCIRLSGCPTLTIKDNPDPLRRDPVATVIEGCVGCGLCGENAHAAVLCP
                     SFYRAEVIQNPTAWDKLVHRIA"
     gene            8896..>9857
                     /locus_tag="VFR57_07340"
     CDS             8896..>9857
                     /locus_tag="VFR57_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEU0259211.1"
                     /translation="MSLPERPITILIAALGGEGGGVMADWLMEAATRSGFPAQATSIP
                     GVAQRTGATTYYLEIYPAQREVLGDKEPVLSLTPSPGNVDVMVASELIEAGRAMLNGY
                     VSPERTTLIASTHRIYATVEKMQMADGRFESERVLEAARELAKHAVLFDMRKLAQESG
                     TVINAVLFGAMAGSGVLPLTRQACEDAIRHGGRGAEASLRGFAAGYEIAVGTRAAPAE
                     AAPPKQARELDEVVTLAVERLRDYQGESYSNLYLERIKPFQAEAKLAAEVARQLALWM
                     SYEDIIRVADLKTRATRFERVRKEVGARDGEPVVVIDYLKPGVE"
BASE COUNT         1690 a         3212 c         3275 g         1680 t
ORIGIN      
        1 gcggtcttcg gtggcgacga gctggttgta ggcctcgatc tcgaagcggt agccggtgag
       61 aagatccatc tgcaggccgt aatgcaccga cttcttcgcc tggcgcaccg cgagcggggc
      121 attgtccgag atagtgcgtg cggtcgcgag cgcgtcgttc agcgcgttgt cgcacacccg
      181 gttaacgatg ccccactcgt agccctgctg cgcatcgaag ggctgggcgg tcatgacgag
      241 ctcctttgcg cgccgctcgc ccgcggcgcg cgcgaggttt tgcgtgccgg cgccgcccgg
      301 catgaggccg agacgcgtct cggagagcgc catgcgcgcg gctttcgccg cgtagatgaa
      361 gtcgcaggcg agcgccatct cgaggccgcc gccgaaggcg tggccattca ccgcggcgat
      421 caccggcaac ggcagctcca tgagcgctac gaaggcgcgc tcgaagatct cgtgctgcac
      481 gcgccagacc gtttccgtca tgccgtcgcg ctccttgaga tcggcgccgg cgcagaacgc
      541 gcgctcgcct gcgcccgtga gcacaacgca gcgcagctcg cccggctctg cggtcagccg
      601 cgtccagagg tcgatcttgt cgcggcccat ctgcgtgctg agcgcattca gcgactgcgg
      661 gcggttgagc gtgacgacga ggacgtgggc gtcgcgcttt tcaagcagga gcgtttgata
      721 ggcgggaaga ttcatcgata gagtttaaac acatgaaggt tctggttctc gtcttgtgtc
      781 tgctgtctgg ctgcgccgtg ctcgagacga aaggcgccgt tgtcggttgc caggcggccg
      841 acaccgtgac gacgttgcat gccgtttcgc tgggggcgcg cgaggcaaac ccggtggttc
      901 gatggctgct cgagacgttc gggccgggcg ggttcatcgc cgcgaaggtc ggcgtgacgc
      961 ttcttttcct gcactactac ccggatatat cgtcggacgt ggtaatcctt gtgaacgggg
     1021 tgacgtgcgc cgtggcagcg cacaacctcc gcgtcgcttc tcagctcccg cggtagagct
     1081 ccaccatcgc ttcgcgcagc cagcggttgc cggggtccgc ttcgaagcgc tcgtgccaat
     1141 gcaacgcgac ttccgccgga ggaatcgcga ccggcggcgg caacgctttc agcttcccgc
     1201 ggcgctcgaa aacacgagcg acgcgcgccg gcagcgtgag gatgaggtcg gtgcgctcga
     1261 gcaccatcgg caccacggtg aagtgcggca cgcgtagcgt aatgcgtcgc gtaagcccgg
     1321 cccggtcgag cgcctcttca atgaccctat ggccgccgcg gtaggtgacg agcgcgtgcg
     1381 atgcttcgag gaagcgcttt ttcgtcagag tggtgatcga gtggtcggcg cgcatgaggc
     1441 agaggtaggg atcgcggaag agagcctggc gccgcaccgg cgagcccagc ccgggcagga
     1501 atccgatcgc cacatcgagc gccccggttg cgagggcatt gccgatgtcg tcgacttcga
     1561 gcgcgaccgc ttcgaggcgc accccgggcg cgtcgcggtg cagtcgctcg acgagtggag
     1621 gcagaaactc gatctggccg aggtcggaca tgtagaagcg gaaagcgcgc gtcgaactcg
     1681 tggggtcgaa gcccggcccg tgcgcaagcg ccgattcgag aagcgcgagc gcttggcgca
     1741 ccggctcggc cagctcgcgc gcgaacggcg tcgcgtcgac gccgcccggc gtgcgcacga
     1801 aaagcggatc gccgaagagc ttgcgcagcc gcgccagcgc gttggagacg gcgggctggg
     1861 tgaggccgag gcgggccgcc gccggcgtga cacccttttc atgcagcacg gcgtcgaaca
     1921 cccggagcag attcagatcg acgtcctgca cattcatgcg agtgattcat atcatgagcg
     1981 acattgattt gacaaatggc gaggcggacg cctacgctcg ccggttcact ataggaggag
     2041 tgatgaggaa accattgctg gtggccgtgg ccgcggcgct gccattggcg gcgcacgccc
     2101 aggaaacgac gctcaagctg gtctcggcgt ttggcgagac cacgacgtac gtgaagcaca
     2161 tgacgccctg gatccagaag ttcaacgccg agggcaaggg cattgcccaa atcaatttca
     2221 tcggcggacc gaaggcgatc ccgaccttcg aggtcggcac atcggtgaag aacggggtgg
     2281 tcgagatggc aatgagcacg ggcgcgttct acacgaacct catgcccgaa gccgatttcc
     2341 tcaagctcac gcagatcacg atcgccgagc agcgcaagaa cggcgcgttc gactacatca
     2401 acaaggtgtg gaaccagaag gccaacatgg cgtatctggc gcgcatggtc gacgagacgc
     2461 ccttccacct ctaccttacg aagaagatcg acaagcccga cctcacgggg ctgaagctac
     2521 gcatcacgcc cgtctatcgc gatttcttcg cctcgatggg cgcgagcctg atccagacgc
     2581 cacccggcga ggtgtacacg gcgcttgagc gcggcgtggt cgacggctac ggctggccga
     2641 tccaaggcat cttcgatctc aactggcacg agagaaccaa gttccgcgtc gatccgggct
     2701 tctataacgc cgaagtgtcg atcgtcatga acctcgacac ctggaagaag ctctcgccga
     2761 aacagcgtga gtacgtccag aagcaggcgc tcgcgctcga gtccatgaat gacgtgtggc
     2821 gcaagctgaa cgccgaggag accaagaagc aggccgagtc gggcatccag gcgatcaagt
     2881 tcgacaacgc gttccgcgag aaggcgtacg aagtggcatg ggcggcggcg acgaagcaga
     2941 gtccggagca cgcgccgcag atgaggaagc tcttctcgaa gtgaaggcgc tctacgcccg
     3001 ggtgctcgag tggctcgccg cctgcgcggc ggtgctgctc ggcgcatcgg cgcttgccat
     3061 cacgatcgat gtgatcggcc gcaacatggg catgggcacg ctgccctgga tcctcgaagt
     3121 ttcggaatac gtgctgccgc tcgccacctt tctcatcgcg ccgtggctcc tctaccgcaa
     3181 tgaccacgta cgcctcgata tcctggctct gacccgtccc tggctcggcc acgtggcgaa
     3241 catcgttggc ctcggcgtgt gcgcggtgct cgtggtgtac ggggtgcgca ccatcctgaa
     3301 ttcggctgca cagggcgcca tgatcttcaa gagcgtggtg tttcccgaat ggtggctgta
     3361 cctgccggtg ccgctctgct tcgggctcct gacgatcgag ttcgtgcggc gcctcgcgcg
     3421 tcgatgagct ggtgggtcgc ggcgtgcatt ctcttcggct ctctctccct cctcttcgcg
     3481 ctggcgctgc cggcggcgtt cgccttcctc ggcatcaaca tcgtcggcgc gtgggtatgg
     3541 ctcggcaagg atgcgggctt gatccagctc gtgcgcaacg gcgtggcgtc gatctcgtct
     3601 ttttcgctga cgccgatccc cttcttcatc ctgatgggcg aggtgctctt ccacaccggc
     3661 gtggcgatga aggcgatcga cgcgatcgat cgcctgatct ggcgcattcc gggacgcctc
     3721 gcggtcgtgg cggtcgtcgc cggaaccgtg ttctcggcca tttccggctc gacgattgcc
     3781 accaccgcgc tcctcggcag cctgctcttg ccgcagatgc tggcgcgcgg ctaccacccc
     3841 aacatggcga tgggcccgat catggcgatc ggcggcgtcg acatgctgat tccgccttcc
     3901 gccctcacgg tgctcctcgg cagccttgcc gggatctcga tctcgcagct cctcgttgcg
     3961 ggcatcgttc ccggcgttct catgtcgtgc ctcttcgtcg gctggatcgt gctgcgcgcg
     4021 agcatggatc cgagcctcgc gccgcgcttc gagcacgaag cgctcacggc atgggcacgg
     4081 ctgaagcccc tgctcctcta tgtcgcgccg ctcgttctca ttttcgtcgt cgtggtggcc
     4141 gcgatggccg caggctgggc cacgccgacc gaagccgcgg ccatcggagc ggtggcgacc
     4201 gtgctcgtcg cctggattta ccgcgcgctc acctggtcga acctgatgcg cgcgctcatg
     4261 gggaccgcgg gcatttccgg gatcatcctc ttcatcatcg tgggcgcgac cacctttgcc
     4321 cagatcctga cgttctcggg cgcgaccaat ggcttggtgg cggcgatcac cgacgcgcag
     4381 ctcgcgccgt ggatcgtgct cgcggcgatg ctcgtcatcc tgctggtgct cggctgcttc
     4441 atcgaccagg tgagcatgat gctcatctgc ctgccgttct tcatgccgct ggtgggcgcc
     4501 tacagcttcg acccgatctg gttcggcgtg ctttttctca tctgcatgca gctcggcctg
     4561 ctgacgccgc cgttcggcat gctgcttttc acgatgaaga gcgtggcgcc gaaggaaatc
     4621 gccatgtcgc aggtatggca cgcggtcacg ccctacgtgg tgatgggcat ggtcgcgctc
     4681 gctctggtca tcgtcttccc gccgctcgcc acgtggcttc ccaaggcgct cattaagtag
     4741 ccatggaacg ctccttcaag gaagaagtcg cgaaattgag gctgggcgat ggcgagacct
     4801 tccacggcga aggcatcctc gccgtcacca aggcgctgct gcagtccggc gtcgcctacg
     4861 tcggcggcta ccaaggcgcg ccggtgtcgc acgtcatgga cgtcctgaac gacgcacgcg
     4921 acatcctcga cgagctcggc gtgcacgtcg agaccaatgc gaacgaggcc ggcgcggcgg
     4981 cgatgctcgg cgcctcgatc aactatccga tgcgcggcgc ggtggcgttc aagtcgaccg
     5041 tcgggaccaa cgtcgcctcg gacgcgctct ccaacctggc ctcggccggc gtgaaaggcg
     5101 gcgccctgct cgtcctcggc gaggactacg gcgagggctc ctcgatcatc caggagcgca
     5161 gccacgcttt cgccatgaag tcgcagatct ggctgctcga tccacgcccc aacctgccga
     5221 agatcgtcga gatggtcgag caaggcttcg agctctccga agcgtcgagc accccggtga
     5281 tgcttgagtt gcgcatccgc gcctgccatg tccaaggcag cttcaagacc cgcaagaatc
     5341 aggcgccgca attctcgaag aaggcgttcc ttcagaaccc cgatttcgac tacggcaaga
     5401 tctgcctgcc acccgcaacg tatgcgcagg agaagcacaa agtcgacgtg cgctggcccg
     5461 cagccgtcca cttcatccgc agccgaaagc tgaatgagtt tttcgacgga gaacaaaaag
     5521 accttggcat cgtctgccag ggcggcctgt acaacgcggt gatccgtgcg ctccagatgc
     5581 tcggcctggc cgacgcgttc ggcgcctcgc gcgtgccgat ctactgcatg aacgtgactt
     5641 acccgctgat cccggacgag atcaccggat tctgccaaga caagaaatcg gtcctcatcg
     5701 tcgaagaggg ccagcctgcg tacatcgagg acgcagtgct cgcggcactg cgcagagccg
     5761 gggccaatag cgtgaaggtg agcggcaagg acgtgctgcc gctcgccggc gagtacaccg
     5821 gcgaagtggt gctgaccggg ctctcgaggt tcctcgatcg ctcggagcgc gttcagccga
     5881 ttctctcgct gaagcaaaag gcttccgggc tcctcgccgg cttgcccgcg cggccgccgg
     5941 ggttctgcgt cggctgcccg gagcgcccgg tattctcggc gatgaaagtg ctggaaaagg
     6001 aaagcggcaa gttccacctc tcggcggaca tcggttgcca tctcttctcc acgcttccgc
     6061 cattcaacat cggcaacacg gtgcttggct acgggcttgg cctcgcctcg aactccgcgg
     6121 tgcacccgat gttcggcaag cgcaccgtga ccatgatggg cgacggcggc ttctggcaca
     6181 acggcctcac caccggcatc gccaacaccg tgttcaacaa gaacgacggc atcctggtga
     6241 tcatgaagaa cggctacagc tccgccaccg gcacgcagga gctgccctcc tcgccgcaac
     6301 acagcgagca caaggcgccc gacatgagca tggagcgcgc gctggagggc atcggggtcg
     6361 gctgggtgaa gaccgtcgac aactaccgcg tcggcgaggt ggcgaagacg ctcaaggagg
     6421 ccatgaccac cgactatcca ggcctcaagg tgatcatcgc cgagggcgag tgccagctcg
     6481 agcgccagcg caagctcagg cctctcgttt caaaacagtt atcggaggga aaacgcacgg
     6541 tgcgcgtgaa gtacggggtc gacgaagaca cctgcacggg cgaccactcg tgcatccggc
     6601 tctcgggctg cccgaccctg acgatcaagg acaacccgga cccgctgcgc cgcgatccgg
     6661 tcgccaccgt gatcgacggc tgcgtcggct gcggcctgtg cggcgagaac gcgcacgcgg
     6721 cagtgctgtg tccctcgttc tatcgcgccg aagtcattca gaacccgacc tggtgggatc
     6781 gtctgtggaa cgcgagttca cccgggaaat agagctcctg cgcctgggcg acggcgacac
     6841 cttccacggc gagggcatcc tcgcggtgac gaaggcgctt ctgcagtcgg gcgtgtccta
     6901 tgtcggcggc taccagggcg cgccggtgtc gcacctgctc gacgtcatgg tgcaggccag
     6961 ggactatctc gacgagctcg gcgtgcacgt cgaggcgtgc accagcgaag cctccgccgc
     7021 cgccatgctg ggcgcctcga tcaactaccc ggcgcgcggc gcggtcacct ggaaatcgat
     7081 cgtcggaacc aacgtcgccg ccgacgccct ctcgtatctc gcgagcgccg gggtgatggg
     7141 cgggacattg atcgtgctgg gcgaggacta cggcgaaggc ggcgtggtcg cccaggagcg
     7201 tagccacgcc ttcgcgctga agtcgctcat gtggcttctc gacccgcggc cgaacctcgc
     7261 gacgatcgcc gagatggtcg agcatggttt cgagctgtcc gaagcgtcga atacaccggt
     7321 gatgatggag ctccggatcc gcgcctgcca tgtgcggggc tcgttcagga caagagcgaa
     7381 cgtggcgcct tccgtttcca cgcggcagcg gctcgacggt ccgccgcgcg agttcatcta
     7441 cgaccggctc gcgcacccgc cggccacctt ccggcaggag aagctcaagt ccgaagtccg
     7501 gctaccggcc gcgcgcaagt acattctgga aaaccgcctg aacgaggttt ttccgggaag
     7561 cgagaacctc ggcatcgtcg tccagggcgg cctgtacaac ggcctcatgc gcgcgctgcg
     7621 cacgctcggg ctcgccgatc cgtttggtgc aacccgcatt ccgatcctag cgctcaacgt
     7681 cgtctatccg ctcgtgccgg aagagatcgt cggcttcgcg agggacaaaa cggcgttgct
     7741 catcctcgag gaaggacagc cggagttcat cgagcaggaa atcgccacct tcctgcgccg
     7801 cgccgacgag ccggcgaagc tgcacggcaa ggactgcatg catatggcgg gcgagtacaa
     7861 cgtcgagtcg ctcgtgcgcg gcatcgcgcg tttcgtcgag ctgcatgcgc ggcacctcga
     7921 cgtttcggcg ggcggccggt ggctggactc ggtgcagaag ggcaagagcg cggcctccag
     7981 cctcctcggc gcattgccgg cgcgaccgcc gacgttctgc gtgggctgcc cggagcggcc
     8041 ggtgttctcg gcgatgaagc tcgtggcgaa ggacatcggc cggccgcatg tcgccatgga
     8101 cgtcggctgc catgcgtttg cgagcttcga gcccttctcg cagggcaata ccctgctcgg
     8161 ctacggcatg agcctggcct ccgccgcagg cgtggcaccg atgcaggcaa agcgcgcggt
     8221 cgcggtcatg ggcgacggcg gcttctggca caacgggctt ctctcgggcg tggcgtcgaa
     8281 cctgatgaac aagggcgacg gcgtgctcgt catcatgaag aacggctacg cctccgcgac
     8341 cgggacgcag gagctcatct cttcgccgcc ggaggaagcg cgccgggaag cgctcggcca
     8401 gagcgccgcg gatgccgatc gcaccatcga aaacacgctg accggcctcg gcgtgaagtg
     8461 gctgcgcacg gtgaataact acgacgtcgc caccatgacg aagacttatc gcgaggccat
     8521 gacgacgccc gacaagggcc tcaaggtgat cgtcgccgaa ggcgagtgcc agctggagcg
     8581 gcaaaggcgg ctcaagcccg ccgtggtcga acggctggcc cggaaggaaa gacacgtgag
     8641 ggtgaagtac ggggtggacg aggacacgtg caccggcgac cactcgtgca tccggctctc
     8701 gggctgcccc acgctcacga tcaaggacaa ccccgacccg ctgcgccgcg atccggtcgc
     8761 caccgtgatc gagggctgcg tgggctgcgg cctgtgcggc gagaacgcgc atgccgcggt
     8821 gttgtgtcct tcgttctatc gcgccgaagt catccagaac cccaccgcgt gggacaagct
     8881 ggtccatcgc atcgcatgag cctgcccgag cgtccgatca cgatcctcat cgccgcgctg
     8941 ggcggcgaag gcggcggcgt catggccgac tggctgatgg aggccgcgac gcgaagcggt
     9001 ttcccggcgc aggcgacctc gatccccggg gtggcgcagc gcaccggggc gaccacctac
     9061 tatctcgaga tctaccctgc gcagcgcgaa gtcctcggtg acaaagagcc ggtgctttcg
     9121 ctcacgccga gtcccggcaa tgtcgacgtg atggtcgcct ccgagctcat cgaggcggga
     9181 cgcgccatgc tgaacgggta tgtgagtcct gagcgcacca cgctcatcgc ctccacgcat
     9241 cgcatctacg ccacggtcga gaagatgcag atggccgatg gccgcttcga gtccgagcgc
     9301 gtgctcgaag cggcgcgcga gctcgcaaag cacgccgtgc tcttcgacat gcgcaagctc
     9361 gcgcaggaga gcggcaccgt gatcaacgcg gtgctgttcg gagcaatggc ggggagcggc
     9421 gtgctgccgc tcactcgcca agcgtgcgag gacgcaatcc gccatggcgg ccgcggcgcc
     9481 gaagctagcc tgcgcggttt tgccgcgggc tacgaaatcg ccgtaggcac gcgtgcggcg
     9541 cccgcggaag ccgcgccgcc aaagcaggcg cgcgagctgg acgaggtggt gacgctcgcg
     9601 gtcgagcggc tgcgcgacta ccagggcgag tcgtactcga acctctacct cgaacgaatc
     9661 aagcccttcc aggcggaagc gaagctggcc gcggaagtcg cgcggcaact cgccctatgg
     9721 atgagctacg aggacatcat ccgcgtcgcg gacttgaaga cccgcgccac gcgcttcgag
     9781 cgggtgcgca aggaggtcgg cgcgagggac ggcgagccgg tggtggtgat cgactacctc
     9841 aagccggggg tcgagga
//
LOCUS       DASTPD010000223         7012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_115595, whole genome shotgun sequence.
ACCESSION   DASTPD010000223 DASTPD010000000
VERSION     DASTPD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115595"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VFR57_07345"
     CDS             complement(<1..128)
                     /locus_tag="VFR57_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259212.1"
                     /translation="MNLELKGRSVLVTGGSKGIGLACAKALAAEGCRVHIASRNKET"
     gene            complement(125..1576)
                     /locus_tag="VFR57_07350"
     CDS             complement(125..1576)
                     /locus_tag="VFR57_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEU0259213.1"
                     /translation="MPYDLLIRNVRVVRPSSVEDADIAITAEKFAKVGRNLGDAKSVY
                     DGRGWLAFPGVVDAHMHTGIYSPLEEDAVSESRAAAQGGVTSSLNYFRTGQYYLNRGG
                     PYRDFFPEVLKTSAGRFHIDYGFHLAPMDAAHIAEIPELVEKHGVSSFKIFMFYGSHG
                     LHGRSDSQRDFLMIGPEERYDYAHFEFVMRAVQAARERFRDKAGQISLSLHCETAEIM
                     TAYTKIVESEGKLDGLAAYHAARPPHSEGLAVFIASYLANEAALANINLLHLSSRKAL
                     EAALKMAEVFPHIDFRREVTIGHLVLDVNSKAGVLAKVNPPIRPREDVEFLWEKVLEE
                     KVDWVVSDHACCRHETKAAKHALGNIWLAKSGFGGTEYLLAALVSEGTKRGLSLNRVA
                     ALTSGNPAKRFGLNSKGDIAPGLDADLALVDPKHTWTIRAKDSESTQGYTPFEGIELS
                     AKVKATFLRGSLICENGNVLGKPKGRYMHRPTP"
     gene            complement(1558..2028)
                     /locus_tag="VFR57_07355"
     CDS             complement(1558..2028)
                     /locus_tag="VFR57_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEU0259214.1"
                     /translation="MFGDDIDTDVIVPGRYMKYSIEEIAQHCFEPIAPHFAKEVKRGD
                     VVVGGRNFGAGSSREQAPAALKQLGVAALIAESFAGLFYRNSLNLGLPAVVCKEAKRI
                     RDGDRLEIDLAQGVVATPRENLSFEPIPPHLMQMVRDGGLLPHLEKRLKCRTTS"
     gene            complement(2037..3272)
                     /locus_tag="VFR57_07360"
     CDS             complement(2037..3272)
                     /locus_tag="VFR57_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0259215.1"
                     /translation="MTLAQKLVARAAGRDRVTPGEIVTCKVDLAMMHDSGGPRRVKPM
                     LERLGAKVWDPSKVVVITDHYVPTYDDESRKIIRLARDWVRDAGIDNFYDEQGICHVV
                     LPERGHLRPGMFAVGGDSHSPTGGAFAAYMFGVGATEMLGVLVTGEIWLKVPHTIAMD
                     FDGALGRGVSAKDVMLFLIGRFGMDGGEYQAVEYRGPAIRSLPMQERMTLCNMTAELG
                     GQTGLIASDETTAAYLGTKVETWESDIGAPVLAHHRFNASELAPQVAAPHSPANAKPV
                     DEFLSVKPTVAYIGACTGAKLADLRMAASVLRGRKAKMRLMVAPASARDVKAAEEEGT
                     LAVLRDAGAEFLGLGCGICAGYGGNVLGENVVAISTTARNFKGRMGAPSSQVFLASPY
                     TVAATAVAGRICDPRELLH"
     gene            complement(3272..3631)
                     /locus_tag="VFR57_07365"
     CDS             complement(3272..3631)
                     /locus_tag="VFR57_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0259216.1"
                     /translation="MNKAEEIVRQYLAAMERRDLAAARALLAPGFHMVFPGARRFDTL
                     EALVEWARGRYRSARKTYDRYETCGDAVYCYGTLNGELLDGSSYSGIRFIDRFTVRDG
                     KLADQNVWNDMAEVLGR"
     gene            complement(3618..4385)
                     /locus_tag="VFR57_07370"
     CDS             complement(3618..4385)
                     /locus_tag="VFR57_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0259217.1"
                     /translation="MTEKFKIWFQGATDRVHMAPYIAKVEAHLKAILDPEFSATFHTT
                     TPPATTTHAITEFRIARNLIRNAVEAERQGYAAMAITHFQDAGLMEVKSVVDFPVLGL
                     GETTLFHACTLGRKLGLVTINPVFIPWHEEQVIRYGLQQRVVGVRAVNATVKDFIDAF
                     ADQGAYDALYPKWERECRALLDAGADVIVPAGGLPMMLYGGKLFEGAPVVNGVTLIAK
                     SAEMAIKLRKLGVGQVSRRSNLVKPPDKALKEFLEQG"
     gene            complement(4378..5154)
                     /locus_tag="VFR57_07375"
     CDS             complement(4378..5154)
                     /locus_tag="VFR57_07375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0259218.1"
                     /translation="MNVLSQLVEAFSSGKIRVVDLTQTLAPEFPSISLPPEMGQAWPF
                     RIEEVSRYDERGPAWYWNNFSCGEHTGTHFDAPIHWISGKDLPHNATDSIPPDRFIGP
                     GCVIDCTKEAAADPDFLLTKPFLLEWEKKHGRIPPRAWVLMRTDWSKREDPVAYQNYD
                     DEGQHTPGPDVDAVKFLVAERDVLGFGTESIGTDAGQAYHLRPPYPCHYYMHGAGRYG
                     LQCLTNLDQLPPTGAVVIAAPLKIRNGSGSPLRVLALIHD"
     gene            complement(5151..6374)
                     /locus_tag="VFR57_07380"
     CDS             complement(5151..6374)
                     /locus_tag="VFR57_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259219.1"
                     /translation="MQRPKRKNPVLRTRLPTLPPAARSRVALGLTAAAAIGRFELQQC
                     RDCGTVLYPPREACQKCLSVRLDWKPQPTGGELIAQTVLHHSNDLFFRERLPWRLGLV
                     KLDCGPTVVAHLHAGALEKVRVRAMLDRAGQAVIVALPQKEHCDMANDRQLREFSCDP
                     RNRKILVTDGKTAVGQSLVQGFAKSGAEIIWVGYAEPWKKFSGFDELEKISQVTLVPL
                     DVTDSKNVKELAAEIAGKVDIVVNNAEHHRTFGISNRQGVETARAEMDVNYFGLLRLA
                     QEFGPAMRSRGADGAASAVAWVNLLSVYALANFPPHGTFSASKAAAHSLAQCLRAEMR
                     PAGVRVVNVFPGPIDDEWNQLLPPPKIAPAALAKSIVAALCEGIEDVYPGDVAQEWLE
                     RWRDNPKALEREISA"
     gene            complement(6355..>7012)
                     /locus_tag="VFR57_07385"
     CDS             complement(6355..>7012)
                     /locus_tag="VFR57_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU0259220.1"
                     /translation="QRANALKYPHALFKKPLSMEEYLAARPIAEPLHLFDCVMPCAGA
                     EGFLVLAEDRARSLGLPCVRLQGTIERHNAFASDPVQMRAGWALDRDDLYAQAGCKPG
                     DIDFVQTYDDYPVISLMQLEDLGFCEKGEGPAFVRQHSFTHDGSFPLNTSGGQLSVGQ
                     AGAGGGFLGMVEAIRQLTGQAAARAVKDAKLGLVSGFGMINYDRGLGSGAAILAAAQA
                     "
BASE COUNT         1161 a         2270 c         2325 g         1256 t
ORIGIN      
        1 gtttccttgt tgcgcgaggc gatgtggacg cggcagcctt cggcggcgag cgctttcgcg
       61 cacgcaagac cgatgccctt cgagccgccg gtgacgagga ccgatctgcc tttcagttca
      121 aggttcatgg ggtcggacga tgcatgtaac ggcctttcgg tttgccgagc acgttgccgt
      181 tctcgcaaat caggctgccg cgcaggaacg tggccttcac cttcgcggag agctcgatgc
      241 cctcgaacgg cgtatagccc tgggtggatt cggaatcctt tgcgcggatc gtccacgtgt
      301 gcttcggatc gacgagcgcg agatcggcgt ccaggcccgg cgcgatgtcg cccttggagt
      361 tcaagccaaa gcgcttcgcg gggttaccgc tggtcagcgc agccacccta ttcagggaaa
      421 gccctcgttt tgtcccctcg gacacaagcg cggccagcag atactccgtg cccccgaaac
      481 ccgatttggc aagccagatg ttgccgagcg cgtgttttgc cgccttggtc tcatgccggc
      541 aacaggcatg gtcgctcacc acccagtcga ccttctcctc gagcaccttc tcccagagga
      601 actccacgtc ctcgcgcggg cggatgggag ggttgacctt ggcgagcacg cctgccttgg
      661 agttcacgtc caggacgagg tgaccgatgg tgacctcgcg acgaaaatcg atatgcggaa
      721 acacctccgc catcttgagc gctgcttcca gcgcttttct ggaggagagg tggagcaggt
      781 tgatgttggc aagcgccgcc tcgttcgcga gataggaggc aatgaagacc gcgagtccct
      841 ccgaatgcgg cggccgcgcc gcgtggtagg cggcaaggcc gtccagcttg ccttcgctct
      901 ccacgatctt tgtgtacgcc gtcatgatct cggcggtctc gcaatgcagc gacaggctga
      961 tctgtcccgc tttgtcgcga aagcgctcgc gcgcggcctg caccgcgcgc atcacgaact
     1021 cgaaatgcgc gtagtcgtag cgttcttcgg gtccgatcat aaggaagtcg cgctggctgt
     1081 cggaacggcc gtgcaatccg tgcgagccgt agaacatgaa gatcttgaat gacgacacgc
     1141 cgtgcttttc cacgagctcg ggaatctcgg cgatgtgcgc cgcgtccatc ggcgcaaggt
     1201 gaaagccgta gtcgatgtgg aagcggcccg cggaagtctt cagcacctcc gggaaaaaat
     1261 cgcgatacgg cccgccgcgg ttcaggtagt actggccggt gcggaagtag ttcaggctcg
     1321 aggtgacgcc gccctgcgcc gctgcgcggc tctcgctgac cgcatcttcc tcgagcggcg
     1381 aatagatgcc ggtatgcata tgcgcatcga ctacgccagg gaaggcaagc cagcccctgc
     1441 cgtcgtagac gcttttcgcg tcgcccagat tgcggcccac ctttgcgaac ttctctgcgg
     1501 tgatcgcgat gtcggcgtcc tccaccgagg agggccgcac cacgcgcacg ttgcggatca
     1561 ggaggtcgta cggcatttca gtcgtttctc gaggtgggga aggaggccgc cgtcgcgcac
     1621 catctgcatg agatgcggcg gaattggctc gaaggacagg ttttcgcgcg gcgtggcgac
     1681 gacgccttgc gcgaggtcga tctccagccg gtcgccgtcg cggatgcgct tcgcttcctt
     1741 gcacaccacc gcgggcaagc cgaggttgag cgagttacgg tagaacaggc cggcgaagga
     1801 ttcggcaatc agcgccgcga cgcccagctg tttcagagcg gcgggcgcct gctcgcgcga
     1861 ggaacccgcg ccgaaattgc ggccgccgac gaccacgtcg ccgcgcttta cctccttcgc
     1921 gaaatgcggt gcgatcggct cgaagcagtg ctgcgcgatc tcctcgatcg aatacttcat
     1981 gtaacgaccc ggaacgatca catccgtgtc gatgtcgtcg ccgaacaccc aggctctcag
     2041 tggaggagct cccgcgggtc gcaaatgcgc ccggcgactg ccgtcgcggc gacggtatat
     2101 ggcgaagcca ggaaaacctg agaggagggc gctcccatgc ggcccttgaa gttgcgcgcg
     2161 gtggtcgaga tcgccaccac gttctcgccg agcacgttgc cgccgtagcc ggcgcagatg
     2221 ccgcacccaa ggccgagaaa ctcggcgccg gcgtcgcgca gcaccgcgag tgtcccttcc
     2281 tcctcggccg ccttcacatc ccgcgcgctg gcgggcgcaa ccatcaggcg catcttcgcc
     2341 ttgcggcccc tgagcaccga agcagccatg cgcaggtccg cgagcttggc cccggtgcac
     2401 gcgccgatgt aggcaacagt gggttttacc gagaggaact catcgaccgg cttcgcattc
     2461 gcgggcgagt gcggcgccgc tacttgcggt gcgagctccg aggcgttgaa gcgatggtgc
     2521 gcgaggaccg gggccccgat atcgctttcc caggtttcca cctttgttcc caggtacgcc
     2581 gccgtcgttt cgtcggaagc aatgaggcct gtctggccgc caagttcggc ggtcatgttg
     2641 cagagggtca tgcgctcctg catcggcagg ctgcggatcg ccggcccgcg gtactcgacg
     2701 gcctggtatt cgcccccatc catgccaaag cggccgatga ggaagagcat cacgtccttg
     2761 gccgacacgc cgcgcccgag cgcgccgtcg aaatccatcg cgatggtgtg cgggacctta
     2821 agccagatct cgcccgtgac gagcacgccg agcatttcag tcgcgccgac gccgaacatg
     2881 tacgcggcga aggcgccgcc ggtcggcgaa tggctgtcgc cgccgaccgc gaacatgcct
     2941 gggcgcagat ggccgcgttc cggcagcacg acatggcaga taccctgctc gtcgtagaag
     3001 ttgtcgatgc cagcgtcgcg cacccagtca cgcgcgaggc ggatgatctt gcggctctca
     3061 tcgtcgtagg taggcacgta gtggtcggtg atcacgacca ccttcgaggg atcccaaact
     3121 ttggcgccga gacgctcgag catcggcttc acgcgccgcg gcccacccga gtcgtgcatc
     3181 atcgcgagat ctaccttgca cgtgacgatc tcgcccggcg ttacccggtc acggcccgcg
     3241 gcgcgcgcca ccagcttttg cgccagggtc atcaccgccc gagtacttcc gccatatcgt
     3301 tccagacatt ctggtcggcg agcttgccgt cccgcacggt gaagcggtca atgaagcgga
     3361 taccggagta agacgaaccg tccagcagct cgccattgag cgtgccgtag cagtagaccg
     3421 cgtcgccgca ggtctcgtag cggtcgtagg tcttgcgcgc agagcggtac cgccctctgg
     3481 cccactcgac gagcgcttcc agcgtgtcga accgccgagc gccgggaaac accatgtgaa
     3541 agccgggggc gagcagcgcc cgcgcggcgg cgagatcgcg tcgctccatc gcggcgagat
     3601 actgccggac gatctcttca gccttgttca aggaattcct tcagcgcctt gtcgggcggt
     3661 ttcacgaggt tcgagcgccg gctgacctgg ccgacaccga gcttgcgcag cttgatggcc
     3721 atctccgcgc tcttcgcgat cagcgtcacg ccgttcacca ccggcgcgcc ttcgaagagc
     3781 ttgccgccgt agagcatcat cggcaggccg ccggccggga cgatgacatc ggcgcctgcg
     3841 tcgaggagcg cccggcactc gcgctcccac tttggataga gcgcgtcgta ggcgccctgg
     3901 tcggcgaaag cgtcgatgaa gtccttcacc gtcgcgttca ccgcgcgcac gccgaccacg
     3961 cgctgttgga ggccgtagcg aatgacctgc tcctcgtgcc aggggatgaa cacggggttg
     4021 atggtgacga ggccgagctt gcgaccgagc gtgcatgcgt gaaagagcgt ggtctcgccg
     4081 aggccgagca cggggaagtc gacgaccgac ttcacctcca tgaggcccgc gtcctgaaaa
     4141 tgcgtgatcg ccatggccgc gtagccctgg cgctccgcct ccacggcatt gcggatcagg
     4201 ttgcgcgcga tgcggaactc ggtgatcgcg tgcgtcgtcg tggccggtgg tgtcgtggtg
     4261 tggaaggtgg cggagaactc cgggtcgagg atcgccttga gatgcgcctc gaccttggcg
     4321 atgtacggcg ccatatgaac gcggtcggtc gcgccctgga accagatctt gaatttttca
     4381 gtcatggatg agcgcgagaa cccttaatgg cgagccgctg ccattacgga tcttgagcgg
     4441 cgcggcgatg accaccgcgc cggtcggcgg caattggtcg aggttggtga ggcactgcag
     4501 gccgtagcgg ccggccccgt gcatgtagta atggcacggg tagggcgggc gcaggtgata
     4561 ggcctggccg gcatcggtgc cgatcgactc ggtgccgaag ccgagcacgt cgcgctcggc
     4621 gacgaggaac ttcacggcgt cgacatccgg gccgggcgta tgttgtcctt cgtcgtcata
     4681 gttctggtac gccaccggat cttcgcgctt ggaccagtcg gtgcgcatga gcacccacgc
     4741 tctgggcggt atgcgcccgt gcttcttctc ccattcgagg aggaacggct tggtcagcag
     4801 gaagtccgga tccgccgcgg cttccttggt gcagtcgatc acgcatcccg gtccgatgaa
     4861 gcgatcgggg ggaatgctgt cggtcgcgtt gtgcggcaga tccttgccgg agatccagtg
     4921 gatcggcgcg tcgaagtggg tacccgtgtg ctcgccacag gagaagttgt tccagtacca
     4981 cgccggtccg cgctcgtcgt agcgcgacac ctcctcgatg cggaatggcc aggcctggcc
     5041 catctccggc ggcagcgaaa tgctggggaa ttcgggcgcc agcgtctgcg tgaggtcgac
     5101 gacccggatc ttgccgctcg aaaacgcttc gaccaactgg gagaggacgt tcatgccgaa
     5161 atttcgcgct ccaatgcttt cggattatcg cgccagcgct cgagccattc ctgcgccacg
     5221 tcgccggggt agacgtcctc gataccttcg cacagcgccg cgacgatgct cttcgcgagg
     5281 gcggccggcg cgatcttcgg cggcggcagg agctggttcc actcgtcgtc gatcggcccg
     5341 gggaagacgt tgaccacgcg cacgccggcc ggtcgcatct ccgcgcgcag gcactgcgcg
     5401 agggagtgag cggccgcctt ggaggccgag aacgtgccat gcggtggaaa gttcgcgagc
     5461 gcgtagacgg aaagaaggtt gacccacgcg acggcgctcg cggcgccgtc ggcgccgcgc
     5521 gagcgcatcg ccggaccgaa ctcctgcgcg aggcgcagca gcccgaagta gttcacgtcc
     5581 atctcggccc gggcggtctc cacaccctgg cggttcgaga tgccgaaggt gcggtgatgc
     5641 tcggcattgt tgaccacgat gtcgaccttg ccggcgatct cggcggcgag ctctttcacg
     5701 tttttagagt cggttacgtc gagcggcacg agcgtgacct gggaaatctt ctcgagctcg
     5761 tcgaagcccg aaaacttttt ccatggctcg gcgtagccca cccagatgat ctcggccccg
     5821 cttttcgcaa acccctggac gagtgactga cccacggcgg ttttgccgtc ggtgaccagg
     5881 atcttgcgat tgcgcgggtc gcagctgaat tcacgcaact gccggtcgtt tgccatgtcg
     5941 caatgctcct tctggggcag cgctacgatg accgcctgcc cggcgcggtc gagcatcgcc
     6001 cgaactctga ccttctccag cgctcctgcg tgcaggtgcg ccaccacagt ggggccgcag
     6061 tcgagcttca ccagtcccag gcgccacggc aggcgctcgc ggaagaaaag gtcgttacta
     6121 tgatgcagca ccgtctgcgc gatcagctcg ccgccggtcg gctgcggttt ccagtcgagc
     6181 ctgaccgaga ggcacttctg gcatgcctcg cgcggcgggt atagcaccgt gccgcagtcg
     6241 cggcattgct gcagctcgaa gcgcccgatc gccgccgcgg cggtgaggcc gagcgcgacg
     6301 cggctgcgcg ccgccggcgg aagcgtcggc agccgcgtgc gaaggaccgg gttcttacgc
     6361 ttgggccgct gcaaggatcg ccgctccgga gcccaggccc cggtcgtagt tgatcatgcc
     6421 gaagccgctg acgagaccca gtttcgcgtc cttgaccgcg cgtgctgccg cttggccggt
     6481 gagctggcgg atggcctcga ccatgccgag gaatccgcct ccggcgcccg cctgaccgac
     6541 cgaaagctgg cctcctgacg tattgagcgg aaagctgccg tcgtgggtga agctgtgctg
     6601 gcgcacgaac gccggtcctt cgcccttctc gcagaagccc aggtcctcca gttgcatgag
     6661 cgagatcacc ggatagtcgt cgtaggtctg gacgaagtcg atgtcgccgg gcttgcagcc
     6721 cgcctgtgcg taaaggtcgt cccggtcgag cgcccagccg gcgcgcatct ggaccgggtc
     6781 ggaagcgaac gcgttatggc gctcgatcgt gccttggaga cgaacgcacg gcaaccccag
     6841 cgaacgcgcg cggtcctccg ccaggacgag aaatccttcg gcgcccgcgc agggcatgac
     6901 gcagtcgaaa aggtggagcg gctcggcgat cgggcgcgcc gcgaggtact cctccatgct
     6961 gagcggcttc ttgaagagcg cgtgcggata cttgagcgcg ttcgcgcgct gc
//
LOCUS       DASTPD010000224         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1343207, whole genome shotgun sequence.
ACCESSION   DASTPD010000224 DASTPD010000000
VERSION     DASTPD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343207"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFR57_07390"
     CDS             <1..455
                     /locus_tag="VFR57_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0259221.1"
                     /translation="EDLDGYAEVRRALATPVAGGENEYTLFGFKELLARRAVDVAQPD
                     LCIAGGFTGCRHIVALAHAHGVQVNPHVWASAVGQAASLQLIAAIPPANHALFAKDIL
                     LEFDTSSHPFREHLTDMPLRQKQGWVEIPEKPGLGIEVSKDVIKRFSA"
     gene            459..1892
                     /locus_tag="VFR57_07395"
     CDS             459..1892
                     /locus_tag="VFR57_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0259222.1"
                     /translation="MKPANLLIIMSDEHDPRWMGCSGSALMHTPNLDRLAARGLRFTD
                     AYTTCPICVPARAAFAVGKYIHQIGFWDNADPYDGSVPSWHHRLRDQGHRVVSVGKLH
                     FRSTDDDNGFSEEIVPMNVFEGKGDLYGLLRGDDMPPRADGKKLAALAGPGESEYTWY
                     DREIASRAQIWLREEARKYPDRPWVLFVSFVAPHFPLTAPPHWYYRYPFERIPMPKLY
                     REKNLHPYVRDQAENTGYDKYFADEAAVKRARAGYLGLVSSLDENIGHILDTLKDCGF
                     ENNTRILYTSDHGDNLGARGLWGKQVLYEEAAKVPMIVAGPDVPAGVRREPVSHVDVY
                     PTVMECVGAEAPDASGFSLLSRIPERTVLSEYHASSSRSGEYMIRHGKWKYVYFVRYP
                     DRPELFDLDSDPEELKDLGGDPKHEQAKKECHEKLLDMLNPKEVDRRAKRRQAELVAA
                     HGGREAVIRRGSFAYSPPPGFKAEFDQ"
     gene            complement(1886..2194)
                     /locus_tag="VFR57_07400"
     CDS             complement(1886..2194)
                     /locus_tag="VFR57_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259223.1"
                     /translation="MLKKLAVLAAALAVSAPAFADRGHGYGRGHAYGHGYGHPERVVV
                     VHKPHYVVHRPAPVVHHYYRPAPRPVYVVEQHNHVNPVAFLAGAVIGAAIVHHAVTHY
                     "
     gene            2313..3278
                     /locus_tag="VFR57_07405"
     CDS             2313..3278
                     /locus_tag="VFR57_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259224.1"
                     /translation="MSRLVLVLLACLLAAAARAQPYPSKPITMIVPFPPGGVADIVGR
                     PLAAQMEKSLKQPVVVTNRTGAGGAVGMAAVAKSVPDGYTILMGLSSISIFPVSDRIM
                     GRTPAYEMKDFAPIALITADPTVLVVGADSPWKTLPEFVASAKAYPGKINYSSSGVYG
                     TLHVAMEIFAHAAGIKLFHVPYQGGGPAVTALLGGQVHALASGPAAAIGQIKAGKMRA
                     LASWSTERLPLLPEIPTFKELGYDAEFYIWSGVFAPAATPAPVIDRLRIAVREAATSA
                     EFKSTMEKVSTPVSYLDAPAFRSYWERDAARLKAALEKIGRVEEK"
     gene            complement(3279..>3767)
                     /locus_tag="VFR57_07410"
     CDS             complement(3279..>3767)
                     /locus_tag="VFR57_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259225.1"
                     /translation="MLAACALWVHGEPPLVLDMRAVRDFDHVVALFRRHGRWGAISKT
                     NGIGLRWRDPVYGSLRELAMSYFHEYTNRRDHKTLREYSVPFDLRRVDPRIWVSGDKQ
                     AWFVAEELDRLKHFKLVDGRHLPAVTRRDPFERRASALLQYRRPRALLQKLARIKKKG
                     RK"
BASE COUNT          666 a         1244 c         1238 g          619 t
ORIGIN      
        1 aggaggatct cgacggttac gccgaagtgc gccgcgcgct cgcgacgccg gtcgcgggcg
       61 gagagaatga atacacgctc ttcgggttca aggaattact tgcgagacga gcggttgatg
      121 ttgcccagcc cgatctatgc atcgccggcg gcttcaccgg ctgccggcac atcgtcgcgc
      181 tcgcgcacgc gcacggcgtg caagtgaacc cccacgtgtg ggcttccgcg gtcgggcagg
      241 cggcttccct gcagctcatc gctgccattc ctccagcaaa ccatgccttg tttgccaagg
      301 acatcttgct ggagttcgat acctcttcgc accccttccg cgagcacctg acggacatgc
      361 cgctcagaca gaagcagggc tgggtggaga tccccgagaa gcccggcctg ggcatcgagg
      421 tttcaaagga tgtgatcaag cgcttttccg cctgacgcgt gaaaccggca aacctgctga
      481 tcatcatgtc ggacgagcac gacccgcgct ggatgggttg ctcgggcagc gcgctcatgc
      541 acacgccgaa tctcgaccgc ctcgcggcgc gcggcctgcg cttcaccgac gcctacacga
      601 catgccccat ctgcgtgccg gcgcgcgctg cgttcgcggt cggcaagtac attcaccaga
      661 tcggcttctg ggacaacgcc gatccgtacg acggcagcgt gccgagctgg caccaccggc
      721 tgcgcgacca gggccaccgc gtggtctcgg tcggcaagct ccacttccgc tcgaccgacg
      781 acgacaacgg tttctcggaa gagatcgtgc cgatgaatgt attcgagggc aaaggcgatc
      841 tctatggcct cttgcgcggc gacgacatgc cgccgcgcgc cgacggcaag aagctcgccg
      901 cgctcgcggg gccgggcgag tccgagtaca cctggtacga ccgcgagatc gcctcgcgcg
      961 cccagatctg gctgcgcgag gaggcgcgga aatatcccga ccggccgtgg gtgctgttcg
     1021 tctccttcgt cgcgccgcat ttcccgctca cggcgcctcc gcactggtac taccgctatc
     1081 ccttcgagcg catcccgatg ccgaagctct accgggaaaa gaatctccac ccgtacgtgc
     1141 gcgaccaggc cgagaacacg ggctacgaca aatacttcgc ggacgaggcg gcggtgaagc
     1201 gtgctcgcgc cggctacctc ggcctggtga gctcgctgga cgaaaatatc gggcacatac
     1261 tcgataccct gaaagattgt ggctttgaaa acaacacgag gatcctttac acctcggatc
     1321 acggagataa cctcggcgcg cgcgggctct ggggcaagca ggtgctctac gaggaagcgg
     1381 ccaaggtgcc gatgatcgtt gccggcccgg acgtccccgc gggtgtgcgc cgcgagccgg
     1441 tgagtcacgt cgacgtctat ccgacggtca tggagtgcgt gggagcagaa gcgcccgatg
     1501 cctcgggatt ttcactgctc agcagaatcc cggagagaac cgtgctgtcg gagtaccacg
     1561 cgtcttcgtc ccgctccggc gagtacatga tccggcacgg gaagtggaag tacgtgtact
     1621 tcgtgcgcta ccccgaccgg ccggagctct tcgacttgga tagcgaccct gaagagctca
     1681 aggatcttgg cggcgatccc aagcacgagc aggccaagaa agaatgccac gaaaagcttc
     1741 ttgacatgtt gaaccccaag gaggtcgacc ggcgcgcgaa gcggcggcaa gcggagcttg
     1801 tcgccgctca cggcggccgg gaggcggtga tcaggcgtgg gagtttcgcc tactcgccgc
     1861 ctcccgggtt caaggccgag ttcgatcagt agtgcgtcac ggcgtgatgc acgatcgccg
     1921 caccgatcac cgccccggcg aggaaggcga ccgggttcac gtggttatgc tgctcgacca
     1981 cataaaccgg gcgcggtgcc gggcggtagt agtgatgcac gaccggggcc gggcgatgca
     2041 ccacatagtg gggtttgtgg accactacca cgcgctcggg gtggccgtag ccatggccat
     2101 aagcgtggcc gcgtccgtat ccatggccgc ggtcggcgaa agccggcgcc gaaaccgcga
     2161 gtgcagcagc aaggacggca agcttcttca acatctgggg cctcctttca agttgagtgc
     2221 cactgcattt cacgcgccca ggcgtcgaaa gcaaaccgat aaagtgcaac atgtgtaaca
     2281 gcctgttacc ttgacgatcc gcgaggagac ctatgtcacg actcgtcctt gtcctgcttg
     2341 cctgcctgct tgctgccgcc gcgcgggcgc agccgtaccc ttcgaagccg atcacgatga
     2401 tcgtgccctt cccgccgggc ggggtcgccg acatcgtcgg ccggccgctc gccgcgcaga
     2461 tggagaagtc gttgaagcag ccggtcgtag tcacgaaccg caccggcgcc ggcggcgcgg
     2521 tcggcatggc ggcagtggcg aagagcgtgc ccgacggcta caccatcctg atggggctct
     2581 cctcgatctc gattttcccg gtgtcggacc gcatcatggg gcgcacgccg gcgtatgaga
     2641 tgaaggactt cgcgccgatc gcgctcatta cggccgaccc caccgtgctc gtcgtaggcg
     2701 cggacagccc gtggaaaacg ctcccggagt tcgtcgcgag cgcgaaagcg taccccggga
     2761 aaatcaacta ctcgtcctcg ggcgtgtacg ggacgctgca cgtcgcgatg gagatcttcg
     2821 cccacgccgc aggcatcaag ctcttccacg tgccctatca gggcggcggg ccggccgtga
     2881 ccgcgctcct cggcggccag gtgcatgcgc tcgcctcggg cccggccgcc gcgatcggcc
     2941 agatcaaggc cggcaagatg cgcgcgctcg cgagctggag caccgagcgg ctgccgctgc
     3001 tgcccgagat tcccaccttc aaggagctcg gctacgacgc cgagttctac atctggtcgg
     3061 gcgtgttcgc gccggccgcg acgccggcgc cggtgatcga ccggctgcgc atcgcggtgc
     3121 gcgaagcggc cacctcggcg gagttcaagt cgacaatgga gaaggtctcg accccggtca
     3181 gctacctcga cgcgccggcg ttccgcagct actgggagcg cgacgccgcg cgcctcaaag
     3241 cagcgctgga gaaaatcgga agagtcgagg aaaagtagtt acttcctgcc cttcttcttg
     3301 atccgcgcca gcttctggag cagcgcccgc ggacggcggt actggaggag cgcgctcgcg
     3361 cgccgctcga acggatcccg gcgcgtcacc gccggaaggt gccggccgtc gacgagcttg
     3421 aagtgcttga ggcgatcgag ctcttcggcg acgaaccagg cctgcttgtc gccgctcacc
     3481 cagatgcgcg ggtccacgcg gcgcaggtcg aacggcaccg agtattcgcg cagcgtcttg
     3541 tggtcgcggc ggttggtgta ctcgtggaag tacgacatcg cgagctcgcg caggctgccg
     3601 tacaccgggt cgcgccagcg cagcccgatg ccgttcgtct tggagattgc gccccagcgc
     3661 ccgtggcggc ggaagagcgc cactacgtgg tcgaagtcgc gcacggcgcg catgtcgagc
     3721 acaagcggcg gctcgccgtg cacccagagc gcgcacgccg cgagcat
//
LOCUS       DASTPD010000225         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1753355, whole genome shotgun sequence.
ACCESSION   DASTPD010000225 DASTPD010000000
VERSION     DASTPD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753355"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFR57_07415"
     CDS             complement(<1..852)
                     /locus_tag="VFR57_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259226.1"
                     /translation="MKHLIAALTLFVPTLAAAQGNLVLYCAPQEEWCRAMVTAFERKT
                     GVKVAMTRKSSGEIYAQVKAEAANPRGDIWWGGTGDPHLQAAEEGLTVEYRSPMEKEL
                     APWAIRQARDAKYRTIGVYAGVLGFGYNTELLAKKKLPEPKCWSDLASDKYRDEVQVA
                     DPNSSGTSYTMLATLVQLHGEDKGFDLMKRMHRNINQYTRSGAAPAKAAASGETLIGI
                     TFMHDLVTFAVQGNPIKVVAPCEGTGYEVGSMSIIKGGKNPKEARMWYDWALTPEAQA
                     LGAETKAF"
     gene            complement(849..1661)
                     /locus_tag="VFR57_07420"
     CDS             complement(849..1661)
                     /locus_tag="VFR57_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0259227.1"
                     /translation="MNEGELDAREAALAAVAREAGELARKYFESGVSTQLKGKQDFIT
                     VADGAVERLIIERLKGRFPRDAFLGEEGGAEGTGDAVWVIDPIDGTANFAHRIPHFCV
                     SIAFISAGQLLLGAIAAPVYGELYRARLGGGAFLNDRAMRVADVSDIRRAIIELGWSA
                     RRPVRAYTGLLERVLDAGAIFMRAGSGALGLAYVAAGRTHGYCELHINSWDTLAGLLL
                     VREAGGWTNDYLAGEALQRGNAVLACAPGLREALVAVTGVGQATENLKRRMS"
     gene            complement(1658..>2350)
                     /gene="iolE"
                     /locus_tag="VFR57_07425"
     CDS             complement(1658..>2350)
                     /gene="iolE"
                     /locus_tag="VFR57_07425"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEU0259228.1"
                     /translation="VSGWYSARLLERSVEDEIKAMQPHTTLLKKLGATAMVFCEVSRC
                     VHGHRKVPLSKRPQLAGKEWMPFLERLDAVARHLEDDGMKLAYHHHMGTVIQSEDEVE
                     RMMDGTKAVGLLLDTGHLTFAGGDPVRALKRHAKRIVHVHCKDIRKDRLEQATKKDWS
                     FLDSVVGDVFAVPGDGSIDFEPILKNLAHAGYAGWLVVEADQDPAIAHPLTHARIGHD
                     CLRPLAQRYWKA"
BASE COUNT          380 a          790 c          747 g          433 t
ORIGIN      
        1 gaacgccttg gtctcggctc cgagcgcctg cgcttccggc gtcagcgccc agtcgtacca
       61 catgcgcgct tccttcgggt tcttgccgcc cttgataatg ctcatcgaac cgacctcgta
      121 gccggtgccc tcgcagggag cgaccacctt gatcgggttg ccctgcaccg cgaacgtgac
      181 gaggtcatgc atgaaggtga tgccgatcag cgtctcgccg ctggccgccg ccttggcggg
      241 cgcggcgccc gatctcgtgt actggttgat gttgcggtgc atccgcttca tgaggtcgaa
      301 acccttgtcc tcgccgtgca gttgcaccag cgtcgcgagc atggtgtacg aagtgccgga
      361 agaattgggg tcggcgacct ggacctcgtc gcggtacttg tcggatgcaa ggtcgctcca
      421 gcacttcggc tcgggcagct tcttttttgc cagcagctcg gtgttgtagc cgaagccgag
      481 cacgccggcg taaacgccga tggtgcggta cttggcgtcg cgcgcttggc ggatcgccca
      541 aggcgcgagc tccttctcca tcggcgagcg gtattccacc gtcaggcctt cttccgcggc
      601 ctgtagatgt gggtcgccgg tgccgcccca ccagatgtcg ccgcggggat tcgccgcctc
      661 ggccttgacc tgggcgtaga tttcgccgct cgacttgcgg gtcatcgcga ccttgacgcc
      721 ggtctttcgt tcgaacgcag tgaccatggc acggcaccat tcctcctgag gtgcgcaata
      781 caggaccaag ttgccttggg ccgctgccag cgtcggaacg aacagcgtca aggctgcgat
      841 taggtgcttc atgacatcct cctcttcagg ttttcggtag cttgcccgac tccggtcact
      901 gcgaccagcg cctcgcgcag gcccggagcg caggcgagga cggcgtttcc gcgctgcagc
      961 gcctcgcccg cgaggtagtc gttggtccag ccgcccgcct cgcgcaccag gagcaagccg
     1021 gcaagggtgt cccaggaatt gatatgcagc tcgcagtagc cgtgcgtccg cccggcggcg
     1081 acgtaggcga ggcccagcgc gccggagccg gcgcgcatga atatcgcccc ggcgtcgagc
     1141 acgcgctcga gcaacccggt ataggcgcgc accggacggc gcgcggacca gccgagctcg
     1201 atgatcgcgc gccggatgtc gctcacgtcg gcgacgcgca tcgcgcggtc gttcaggaac
     1261 gcgccgccgc cgaggcgcgc gcggtagagc tcgccgtaca ccggcgcagc gatcgcgccg
     1321 aggagaagct ggcccgccga gatgaacgcg atcgaaacgc agaaatgcgg aatgcggtgc
     1381 gcgaaattcg cggtaccgtc gatcggatcg atcacccaga cggcgtctcc cgttccctcg
     1441 gcgccgccct cctcgccgag aaacgcgtcg cgcgggaagc gccccttcag gcgctcgatg
     1501 atgagacgct cgaccgctcc atcggcgacc gtaatgaaat cctgcttacc ctttagctgg
     1561 gtggaaacac ctgactcgaa gtacttcctg gcaagctccc ccgcctctcg ggcgacagcg
     1621 gcgagcgcag cctccctcgc atcgagctcc ccctcgttca tgctttccag tatcgctgcg
     1681 cgagtggcct gaggcaatca tggccgatgc gcgcatgggt aagagggtgc gctattgcgg
     1741 gatcctggtc ggcttccaca accagccacc cggcatatcc ggcgtgcgcc aggttcttga
     1801 ggatcggctc gaaatcgatc gagccgtcac cgggaaccgc gaacacatct ccaaccaccg
     1861 agtcgagaaa gctccagtcc ttcttcgtcg cctgctcgag cctgtccttg cggatgtcct
     1921 tgcaatggac atgcacgatg cgcttcgcgt ggcgcttgag cgcacgcacc ggatcgccgc
     1981 cggcaaacgt caggtggccg gtgtcgagca agaggccaac ggctttcgtg ccatccatca
     2041 tgcgctcgac ctcgtcctcg gactggatca ccgttcccat gtggtgatgg taggcgagct
     2101 tcatcccgtc gtcttcgagg tgacgcgcaa ccgcgtcgag ccgctcgaga aatggcatcc
     2161 attccttgcc ggcgagctgg ggtcgcttgg acagaggcac cttgcggtgc ccgtgcacgc
     2221 agcgcgatac ctcgcaaaag accatggcgg tggcgccaag cttcttgagg agagtggtat
     2281 gcggctgcat cgccttgatc tcgtcttcca ccgagcgctc gagcagcctc gcgctgtacc
     2341 agccggagac
//
LOCUS       DASTPD010000226        12125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1143478, whole genome shotgun sequence.
ACCESSION   DASTPD010000226 DASTPD010000000
VERSION     DASTPD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143478"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFR57_07430"
     CDS             <1..768
                     /locus_tag="VFR57_07430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU0259229.1"
                     /translation="YHGAPTAVTLIVGSAPKLAAFAMAIRLLVNGLIDLAQDWQQMLA
                     LLALLSMALGNITAIAQSNLKRMLAYSTIAHMGFMLLGLLSGVVEGNRLNVPGAYSSA
                     LFYMIVYVLMTLGAFGVLLYLSRAGFECENLDDLKGLNRTNPWYAFLMLILMFSLTGI
                     PPTAGFYAKLAVLSAAVNAGQIWLAVSAVIFSLIGAFYYLRIVKLMYFDEAKERVRAT
                     GSTGFAIALSANGLALLVLGIAPWPLMNMCFEAIKAL"
     gene            746..1294
                     /locus_tag="VFR57_07435"
     CDS             746..1294
                     /locus_tag="VFR57_07435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0259230.1"
                     /translation="MKPSKPSDLTEHFVSGSRVFDGNLLKVHRDTVRLPDGTQGTREY
                     IRHPGAVAILALFDDGSVLLERQFRYPHKRDFIEVPAGKLEAGEPPLETGKRELLEET
                     GYVAAEWTPLGVIHTAIAYTDEAIHLFLAKTLTKSKAKLDAGEFLEALRVPYDDALAM
                     IREGRISDCKTVAALLWFKTLA"
     gene            complement(1291..1920)
                     /locus_tag="VFR57_07440"
     CDS             complement(1291..1920)
                     /locus_tag="VFR57_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0259231.1"
                     /translation="MGTPLAAAGAPEDSMLNIAIIVGSTRPGRNGEAVARWVHAIASK
                     RKDAQYELVDIAEFNLPLLDEPVPASMNKYSKPHTKAWAQRVAPFDGFVFVTPEYNHG
                     TSGALKNAIDYLYVEWNNKAAGFVAYGSAGGTRAVEQLRLVMAELQIADVRAQVALSL
                     FTDFENFSTFKPGAHQEKAVGTMLDQVVAWSGAMRALRTKAGELQRLAA"
     gene            1933..2628
                     /locus_tag="VFR57_07445"
     CDS             1933..2628
                     /locus_tag="VFR57_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0259232.1"
                     /translation="MIELRRSTERGHANHGWLDSYHSFSFADYYDPRHMGFGPLRVIN
                     EDRVQPGSGFGTHGHRDMEIITYVLEGALGHKDSMGNGSTIAPGDVQRMSAGSGVRHS
                     EFNYEKAGVTHFLQIWIEPNVTGIAPSYEQKRFEPAEKRGRLRLIASADGSDGSVTIH
                     QDALVYAGLFDGTERARLELQNELGYVHVARGTITVNGQTLRAGDALKTDERAVEIKD
                     GKDAEVLVFDLPA"
     gene            complement(2641..2799)
                     /locus_tag="VFR57_07450"
     CDS             complement(2641..2799)
                     /locus_tag="VFR57_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259233.1"
                     /translation="MRDSIIYLVLTGGAITAADISGLFTYARAAEVAACIARNIDFQH
                     LSVLSICY"
     gene            3039..3977
                     /locus_tag="VFR57_07455"
     CDS             3039..3977
                     /locus_tag="VFR57_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259234.1"
                     /translation="MPQWARGQADQRVHRLGWLSASKLQHKEPYGLAFVQRLRELGFL
                     DGGNLTIEYRHTDSRIEMLPSAAAELARLNVDIFFGAGPEASLVALKQVGANKPIVFV
                     AIDFDPIATGHVVNPARPGGRLTGVTAVQSVLPGKRLELVKELLPATRKVAVLGNDQT
                     TGQLAVVEEAAKRLALELHVIHFKRLPFDYEGAIAEAVRAKSEVLFVLGSALFVPARR
                     LIPELALKARLPSVFHHAQWVEVGGLMSYGFSFPQLWRSAADMVAKIMRGTKAGDIPM
                     EQPTTYELAVNARTAKALGIRIPESIRIRVDRVIDH"
     gene            4246..4431
                     /locus_tag="VFR57_07460"
     CDS             4246..4431
                     /locus_tag="VFR57_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259235.1"
                     /translation="MAAAFAVATVSPVAFAQTKDGKADASKTAALEAACKGKKAGDVV
                     KVDGKDTKCPEAKAAKK"
     gene            4533..5378
                     /locus_tag="VFR57_07465"
     CDS             4533..5378
                     /locus_tag="VFR57_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU0259236.1"
                     /translation="MKSETDLLRRLTDGARAFSANQRSLAKYVQTHYQTVAFSTVSDL
                     AKQSGVSQATIVRFAKALGFKGYPGFQKEVRRLVRADLKGTERFKLSPAAASPLDLVL
                     AKEMENLGALRDAFDAHAFRAALKSLRSASEVLIVGARATAPLATHMAFGLAKLGMRA
                     ARALAVTSDTYDQTNRLDRRACVVVIGFPRYLRELVELLDFAKSAKLATLAITDSAFS
                     PLRGDVSLYAPAESASFMAFHSAPLVLINALLHELSFADRGATVAALDRFEAVADHRN
                     FFHKE"
     gene            5384..6397
                     /locus_tag="VFR57_07470"
     CDS             5384..6397
                     /locus_tag="VFR57_07470"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0259237.1"
                     /translation="MKRIVAALLAAFAGSVAAQSGKPINLALGSVSGSSSIYAFSVAL
                     ANTVRKHDPGLNVTTVEGGGAFDHAKLIKQGVLDWSVSGGPAVVAAVREGGGNFKKEG
                     AWEPARLMFMRNIGISRVYVRADVAKKENIRTWSDLAGKRFSPGIPGTRDMARAMEAN
                     QLLGSKINMIPGSFDDALRRIKEGGLVGMLKGGPHDRFDSGMLEVHHGTPLTVIGFTK
                     DQADKITANDPYNTFLLTPAGGVREAPGTGPFYEMSAPVMVMSSSRMSQETGYRIMKA
                     VMKGWKEIGEAYPPSQGIHPIEDAFKQTPEVKEVLFHAGVIQMAKELNISIPQRLIPP
                     EYK"
     gene            6394..8202
                     /locus_tag="VFR57_07475"
     CDS             6394..8202
                     /locus_tag="VFR57_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEU0259238.1"
                     /translation="MIFWAGLALALFQLFVPVLSDLFDMQLRALHVALATCVILLAVP
                     LSRVWDYALMAVVIAANAVIFIHWQDIITYPGNATTWELVLGASLIAILIDAARRAAG
                     WAIPVMVILMFVYVFAGAYMPGMWAHPGFPLEHVIETLYYSASGIYGSLTGTSATFIA
                     MFILFGALLEVTGGGKTFMDFALWAAGRMKGGPAQVCVAASAGFGMISGSAVANVSVT
                     GNYTIPLMRKLGYHPNFAGGVESMSSIGGSLSPPIMGIASFIMADLLAVPYIEVMKYA
                     IIPCVLFYTGIIAGIHFEASRIGLAPLPPQDIPRLRDIATWANTVPLIIPVIVLMGLL
                     FADYTLLTSGFYSCLAAIALYVFSSRTGIRERAVATVRALSEGGVAVAQIAPLLIAVA
                     MFTALLGLTGVAAKISAVILEMGGDYLFVSLLIAAIIPLLLGAPLPVAATYILSAALI
                     APALIRLKLDPAAVHMFLLYWATLASVTPPTCTASVVAARIAGGDWFKTSLVGMRLGI
                     VAFIVPFFFVVNPSLIWKGPALHIVFHAVAGLAGAMFLAGGFFGYVVGHLSWIMRGVY
                     LVAGGLLLAPDRRLVAAGAVLAALALLTEFLLRRRK"
     gene            8199..9545
                     /locus_tag="VFR57_07480"
     CDS             8199..9545
                     /locus_tag="VFR57_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEU0259239.1"
                     /translation="MKQNLRVMISTGHLGTAPSSPESYYRGLETNPDYVVADGGSSDP
                     GPVYLGEDINLGQFAREEMELFVPSTRKKGIPLIIGSAGDSGSNRGVDEFVGMVKEIA
                     REQRLPKFKVGYFYSEVPKALVKSKLGKSEGLGGFPALTGEEVDNATRIVAVAGAHPY
                     MKLLDMGADVIIGGRSGDIAWTAGPCIRAGFPEGLAYHMGKMIECASLVAEPFMGKET
                     VIGTITKDDIRITPYHPDQRCTIASVAGHSMYERETPYFEHALGGTLEMRECHYEQAD
                     ARTVRITGAKWLPAKELRVKLEGAKKIGERYMGFVGVRDPHVVKNIDKAIEWSKTAVA
                     KRFPDTKYELYFHVFGRDAILKELEPVKAQPAHELGLVVEGVAATDALAEKITDFATR
                     MFFLSRIPGVKGTAGTAATTKKTMKSSPGYMWNVNHTIPIDDPLELFPLHMTEAGV"
     gene            9542..9850
                     /locus_tag="VFR57_07485"
     CDS             9542..9850
                     /locus_tag="VFR57_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEU0259240.1"
                     /translation="MRLYDLASTIRSKNAGVNQITFDIIFPDRKTYERAVRSGAITRD
                     SIAKLYKIPESRISDFVTFDVGNAIKFTIYRLRPSGSPGDWDILGCQQYGPLLDLEIP
                     "
     gene            complement(9847..>12125)
                     /locus_tag="VFR57_07490"
     CDS             complement(9847..>12125)
                     /locus_tag="VFR57_07490"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEU0259241.1"
                     /translation="KDHGDLTNFNISIGVTDAFMQAVEDDTDVELVHKAEPNTDMKSA
                     GAFQREDGLWVYRKVRARELWDEIMRSTYDHAEPGILFLDRINIDNNLRYCESIEATN
                     PCAEQPLPPYGCCCLGSIDLTKFVRNEFSENASFDFERFGKVVALAIRMLDNVLEVTA
                     WPLEQQHKEAMAKRRVGLGFTGLGDALIMLALKYDTAEARAMAARISEAMRDHAYRAS
                     GELAKERGAFPLFNADLYLSGPGFAGRLPEEIKKLIRSNGIRNSHLLSIAPTGTISLA
                     FADNASNGIEPPFSWTYIRRKRVDSGWQEYTVEDHAHRRYRAMGGDVEALPPYFVTAL
                     EMSAKAHQQMVAAVAPFIDTSISKTVNVPEDYPYSEFQDLYFDAWKSGLKGLATYRPN
                     KVLGSVLSVTAPQDFAQDDVNRRISVKSVPAPVLASLRWPGRPDLSGGNPAWTYMIEH
                     PQYRFAVFVGHVENGRPHAFEVWVNGSEQPRGLGALAKTLSMDMRANDSDWLKLKLET
                     LAKAGGDEPFEMAFPPRGDKKLMPSVVSAFAQIVRWRVEQLNLGLAEETFQLKAEQAT
                     SPVLDAMFALKEPKTGTDGTMSWTVDVFNPRTGDDFVLGLKEITLPDGITRPYSVWLS
                     GVYPRALDGLCKILSLDMRVLDPAWVGMKLKKLLEFPEPLGDFMAFVPGSRKQQNFPS
                     TVSYVARLILHRYAMLGILDEDGRPKKQMGILEAPAAPATAPVMSGAKCPECANPTLI
                     RKDGCDYCTACGYVGVCG"
BASE COUNT         2093 a         4004 c         3859 g         2169 t
ORIGIN      
        1 taccacggcg cgcccaccgc ggtgacgctg atcgtcggct cggcgcccaa gctcgccgcc
       61 ttcgccatgg cgatacggct gctggtcaac gggctcatcg acctggcgca ggactggcag
      121 cagatgctcg cgctgctggc gctgctctcc atggcgctcg gcaacatcac cgcgatcgcg
      181 cagtcgaacc tgaagcgcat gctcgcctac tcgaccatcg cgcacatggg cttcatgctg
      241 ctcgggctgc tctcgggcgt ggtcgaaggc aaccgcctga acgtgccggg cgcctattcg
      301 agcgcgctgt tctacatgat cgtgtacgtg ctgatgacgc tcggcgcttt cggcgtgctg
      361 ctctacctct cgcgcgccgg cttcgagtgc gagaacctcg acgacctgaa gggactgaac
      421 cgcactaacc cgtggtacgc gttcctcatg ctgatcctca tgttctcgct caccggcatc
      481 ccgccgaccg ccggcttcta cgcgaagctc gcggtgctct ccgcggcggt gaacgccggg
      541 cagatctggc tcgcggtctc ggcggtgatc ttttcgctca tcggcgcgtt ctactacctg
      601 cgcatcgtca agctcatgta cttcgatgag gcgaaggagc gcgtgcgcgc cacgggctcc
      661 accggcttcg cgatcgcgct atcggcgaac ggcctcgcgc tcctggtcct gggcatcgcg
      721 ccctggccgc tcatgaacat gtgctttgaa gccatcaaag ccctctgacc tgaccgagca
      781 cttcgtgagc ggcagccggg ttttcgacgg caacctgctc aaggtgcatc gcgacacggt
      841 gcgcctgccc gacggcacgc aaggcacgcg ggaatacatc cgccaccccg gcgcggtcgc
      901 gatcctcgcg ctgttcgacg acggctcggt gctgctcgag cgccagttcc gctacccgca
      961 caagcgcgac ttcatcgagg tgcccgcagg caagctcgag gccggcgagc cgccgctcga
     1021 gaccggcaag cgcgagcttc tcgaggagac cggctacgtc gcggccgagt ggacgccgct
     1081 cggcgtgatc cacacagcga tcgcgtacac cgacgaagcg atccacctct ttctcgccaa
     1141 aacgctaacc aaatccaagg ccaagctgga cgcgggcgag tttctcgagg ccttgcgcgt
     1201 gccctacgac gacgcgcttg cgatgatccg cgagggccgc atcagtgact gcaaaaccgt
     1261 ggcggcgctg ctttggttta agacgctcgc ctaagccgcc aggcgctgaa gctcccccgc
     1321 cttcgtacgc agcgcccgca tcgccccgct ccaggcgacg acctggtcca gcatggtgcc
     1381 gaccgccttt tcctgatgcg cgccgggctt gaaggtgctg aagttctcga agtcggtgaa
     1441 cagactgagc gccacctgcg cgcgcacgtc ggcgatctgc agctcggcca tgacgaggcg
     1501 cagctgctcg accgcgcggg tgccgccggc gctgccgtaa gcgacgaagc ccgccgcctt
     1561 gttgttccac tcgacgtaga ggtagtcgat cgcgttcttg agcgcccccg aggtgccgtg
     1621 gttgtactcg ggggtgacga agacgaagcc gtcgaacggc gcgacgcgct gcgcccacgc
     1681 cttggtatgt ggcttggagt acttgttcat cgacgccggc accggctcgt ccaggagcgg
     1741 caggttgaac tccgcgatgt cgacgagctc gtactgcgcg tccttgcgct tcgaggcgat
     1801 ggcgtggacc cagcgtgcga cggcttcgcc gttgcgaccg ggacgggtgc tgcccactat
     1861 gatggcgatg ttgagcatgg aatcctccgg agctcccgcc gccgcaagcg gcgttcccat
     1921 ggacctttcg aaatgatcga acttcgccgc agcaccgagc gcggccacgc gaaccacggc
     1981 tggctcgact cgtatcacag cttttccttc gctgattact acgacccgcg ccacatgggc
     2041 ttcgggcccc tgcgcgtgat caacgaggac cgcgtgcagc ccggctcggg cttcggcacg
     2101 cacggccacc gcgacatgga gatcatcacc tacgtgctcg agggcgccct tggccacaag
     2161 gattcgatgg gcaacggctc caccatcgcg cccggcgacg tgcagcgcat gagcgccggg
     2221 agcggcgtgc ggcatagcga gttcaactac gagaaggcgg gcgtcacgca cttcctgcag
     2281 atctggatcg agccgaacgt caccggaatc gcgccgtcgt acgagcaaaa gcgcttcgaa
     2341 ccggccgaga aacgcggccg cctgcgcctc attgcctccg cggatggaag cgacggctcg
     2401 gtcaccatcc accaggatgc gctggtctat gccggacttt tcgacggcac cgagcgtgct
     2461 cggctcgagc tacagaacga gttgggctac gtgcacgtcg cgcgcgggac cattaccgtg
     2521 aacgggcaga cgctcagggc cggtgatgcg ctgaagacag acgagcgcgc cgtcgagatc
     2581 aaggacggca aggacgccga ggtgctggtg ttcgaccttc cggcttaaac cggtgctctc
     2641 tcaatagcag atcgaaagca ccgacaggtg ttgaaagtca atatttctgg ctatgcaggc
     2701 cgccacctcg gcagcgcgcg cataggtgaa caagccggat atatcggcgg cggtgatagc
     2761 accgcctgta agcaccaggt agatgatcga atcgcgcatc ggctattaaa actacggacc
     2821 aggtccagcg ggcacattac caagccttct gcgcattcac tgttagtcat tgttcgtcgg
     2881 gcgtagtaaa cctttgtaat ctagggcccc tcgtccttgg caaccgcgtt catcgctcgg
     2941 tcaccgaggg gatatcatct gccacccatc tctcgtaacg agctgccata agaccgctct
     3001 cccgccgcac cttcgtcgcg cttacggcag cgctcgcggt gccccaatgg gctcgcggcc
     3061 aagccgatca gcgcgtccat cgtctggggt ggctcagcgc cagcaagcta cagcacaagg
     3121 agccgtatgg cctcgcattc gtgcagcgcc tacgggagct cggcttcctg gatggtggca
     3181 acctgacaat cgaatatcgg cacactgaca gcaggattga gatgctgccg tcggcggcag
     3241 ccgagctggc ccggctgaac gtcgacatat tctttggcgc agggccggaa gcatcgctcg
     3301 tcgctttgaa gcaggtcgga gcgaacaagc cgatagtctt cgtggccata gactttgacc
     3361 caatcgccac cggccacgtc gtcaatcccg cgcgccccgg cggccggttg acaggcgtca
     3421 ccgcggtgca gtccgtgctg cccggcaagc gtctggagct cgtaaaagag ttgcttcccg
     3481 cgacgcgaaa ggtggcggtg ctcggcaatg accagaccac cggccaactt gccgtagtcg
     3541 aggaggccgc taaacggctc gcgctggaac tgcacgtgat tcacttcaag cgccttccat
     3601 tcgactacga aggcgccatc gcggaggcgg tgcgggcgaa atccgaagtg ctcttcgttc
     3661 taggatccgc cctgttcgtg ccggcgcggc gcctgattcc cgagctagct ttgaaggccc
     3721 gcctgcccag cgtgttccac cacgcgcagt gggtcgaggt cggcggtctc atgtcctatg
     3781 gcttcagctt cccgcagctg tggcgcagtg ccgccgatat ggtggcgaag atcatgcgcg
     3841 gaaccaaagc gggtgacatc ccgatggagc agcccactac gtatgagctt gcggtcaatg
     3901 cccggactgc caaagcgctc ggcatccgca tcccagagtc gatccgcatc cgggtcgacc
     3961 gggtgatcga ccactagccg gttcgcttga tttcggccga tcattacccg ccgctcgcct
     4021 ggaagctgac gggctggaac gacaggcgga gttccatatc gtcgggcttc tttacatttc
     4081 gcccgtgcgg cgcttgaacc gtgagtatcg cgcggcttgg caggacctga tttcaggctt
     4141 gcggtcaacc aaccgtcctg ctcgcccgtt atgggcgttg accgagaacc ggtcgttccc
     4201 atcaaccagg aggattactg agtgagcaaa ctaactagtg ctgttttggc cgccgccttc
     4261 gccgtggcaa ccgtttcccc ggtcgcattc gcgcagacga aagacggtaa ggcggacgcg
     4321 tcgaagaccg ccgcccttga ggccgcctgc aagggcaaga aagccggcga tgtcgtcaag
     4381 gtagatggca aagatacgaa gtgcccggaa gcaaaggccg cgaagaagta atcaagctgt
     4441 ttcgtccgac aaaggagggc tcggccctcc ttttttttga catcgattgt ttcaccggcg
     4501 tagcatgatg aaggatttat ttcaccgccc gcgtgaaaag cgaaaccgac ctgctgcgcc
     4561 gcctgaccga cggggcgcgt gctttctcgg ccaaccagcg ctcgctcgcc aagtacgtcc
     4621 agacccatta ccagacggtc gcattctcga ccgtcagtga cctcgccaag cagtctggcg
     4681 ttagccaagc aacgatcgtg cgcttcgcca aggcgctcgg cttcaagggc tatccgggtt
     4741 tccagaagga ggtgaggcgc ctcgtgcgcg ccgacctcaa gggcaccgag cgcttcaagc
     4801 tcagcccggc ggccgcgagc ccgctcgatc ttgtgctcgc caaggaaatg gagaacctcg
     4861 gcgcgctgcg cgatgctttc gacgcccacg cgttccgagc cgccctcaag agcctgcgct
     4921 ccgcgtcgga ggttctcatc gtaggcgcgc gtgccactgc gccgctcgcc acgcacatgg
     4981 ccttcgggct cgcgaagctc ggcatgcgcg cggcccgcgc cctagccgtg acgagcgata
     5041 cctacgacca gaccaatcgc ctggaccggc gcgcctgcgt cgtggtgatc ggattcccgc
     5101 gctacctgcg ggagctcgtc gagcttctcg acttcgccaa gagcgcgaag ctcgcgacgc
     5161 tcgccatcac cgacagcgct ttctcgccgc tgcgcggcga cgtcagcctc tacgcgccgg
     5221 cggaatcggc ttcgttcatg gctttccact ccgcgccgct cgtcctcatc aatgcgttac
     5281 tgcacgagct gagcttcgcc gaccgcggag ccacggtggc cgctttggac cgcttcgagg
     5341 cggtcgcgga ccaccgcaac ttcttccaca aggagtaaat ggcatgaaga gaatcgtcgc
     5401 ggcgcttctt gccgcgttcg cgggctccgt agcggcgcag agcggcaagc cgatcaacct
     5461 cgcgctcggc agcgtttcgg gctcttccag catctacgcg ttctcggtcg cgctcgccaa
     5521 cacggtgcgc aagcacgatc ccggcctcaa cgtcaccacg gtcgagggcg gcggcgcctt
     5581 cgaccacgcc aagctcatca agcagggtgt gctcgactgg tcggtgagcg gcggcccggc
     5641 ggtggtcgcg gcggtacgcg agggcggcgg caacttcaag aaggagggcg cgtgggagcc
     5701 ggcgcggctc atgttcatgc gcaacatcgg catctcgcgc gtgtacgtgc gcgcggacgt
     5761 cgcgaagaag gagaacatcc gcacctggtc cgacctcgcc ggcaagcgct tttcgccggg
     5821 tatccccggc acgcgcgaca tggcgcgcgc catggaggcg aaccagctcc tcggcagcaa
     5881 gatcaacatg atccccggct cgttcgacga cgcgctgcgg cgcattaagg agggcgggct
     5941 ggtgggcatg ctcaagggcg ggccgcacga ccgcttcgac tccggcatgc tcgaggtgca
     6001 ccacggcacc ccgctcacgg tgatcggctt caccaaggac caggccgaca agatcaccgc
     6061 aaacgacccg tacaacacct ttctgctcac gcccgctggc ggggtacgtg aggcgccggg
     6121 cacggggccg ttctacgaga tgagcgcgcc cgtaatggtc atgtcgagct cgcgcatgtc
     6181 gcaggagacc ggctaccgga tcatgaaggc ggtcatgaaa ggctggaagg agatcggcga
     6241 agcatatccg ccgagccagg gcatccaccc gatcgaggac gcgttcaagc agacgcccga
     6301 agtcaaggaa gtgctcttcc acgccggcgt gatccagatg gcgaaagagc tgaacatcag
     6361 tatcccgcaa cgcctgatcc cgcccgaata caaatgattt tctgggcagg gctcgcgctc
     6421 gccctgttcc agctgttcgt gccggtcctc tcggacctgt tcgacatgca gttgcgcgcg
     6481 ctgcacgtcg cgctcgcgac ctgcgtgatc ctgctggccg tgccgctttc ccgggtgtgg
     6541 gactacgcgc tcatggcagt ggtgatcgcg gcgaacgcgg tgatcttcat tcactggcag
     6601 gacatcatca cttaccccgg caacgccacg acctgggagc ttgtcctcgg cgcgagcctg
     6661 atcgcgatcc tcatcgacgc ggcgcgccgc gcggcaggct gggccatccc ggtgatggtg
     6721 atccttatgt tcgtctacgt cttcgccggc gcctacatgc cgggcatgtg ggcgcacccc
     6781 ggcttcccgc tcgagcacgt gatcgagacg ctgtactact cggcttccgg tatctacggc
     6841 tcgctcaccg gcacctcggc aacgttcatc gcgatgttca tcctgttcgg cgcgctgctc
     6901 gaggtcacgg gcggcggcaa gaccttcatg gacttcgcgc tctgggcggc ggggcgcatg
     6961 aaaggcgggc cggctcaggt ctgcgtcgcc gcgagcgcgg gcttcggcat gatctcggga
     7021 agcgcggtgg cgaacgtgtc cgtgaccggg aactacacca tcccgctcat gcgcaagctc
     7081 ggctatcacc cgaacttcgc cggcggcgtc gagtcgatgt cctcgattgg cggcagcctc
     7141 tcgccgccga tcatgggcat cgcgtccttc atcatggccg acctcctcgc ggtgccctac
     7201 atcgaggtga tgaagtacgc gatcatcccg tgcgtcctct tctacacggg catcatcgcc
     7261 ggcatccact tcgaggcgag ccgcatcggc ctcgcgccgc tgccgccgca ggacattccg
     7321 cgcctgcgcg acatcgccac ctgggcgaac accgtcccgc tcatcatccc cgtgatcgtg
     7381 ctgatggggc tgctcttcgc cgactacacg ctcctcacgt ccggctttta ttcctgcctc
     7441 gcggcaatcg cactctacgt tttctcctcc cgcaccggca tccgtgagcg cgccgtcgct
     7501 acggtaagag cgctctccga gggcggggta gcggtggcgc agatcgcgcc gcttctcatt
     7561 gcggtggcga tgttcacagc gcttctcggc ctcaccggcg tggcggcgaa gatcagcgcc
     7621 gtcatcctcg aaatgggtgg cgactacctc ttcgtctcac tcctcatcgc ggcgatcatt
     7681 ccgctccttc tcggcgcgcc gctgccggtg gcggcgacct acattttgtc ggcagcgctg
     7741 atcgccccgg cgctaatacg gctcaagctc gatccggcgg cggtgcacat gtttctgctt
     7801 tactgggcga cgctcgcttc cgtcacgccg ccgacatgca cggcgtcggt ggtcgcagcg
     7861 agaatcgccg gcggcgactg gttcaagacc tcgctcgtcg gcatgcgcct gggcatcgtg
     7921 gcgttcatcg tgccgttctt cttcgtcgtg aatccctcgc tgatctggaa gggcccggcg
     7981 ctccacatcg tcttccacgc ggtggccggg ctcgccggcg cgatgttcct ggccggcggc
     8041 tttttcggct acgtggtggg acacctgagc tggatcatgc ggggcgtgta cctcgttgcc
     8101 ggtggcctcc tgctcgcgcc cgacaggcgc ctcgtcgccg ccggcgccgt gctcgcggcc
     8161 ttggcgctcc taaccgaatt cctgctcaga aggcgtaaat gaaacagaac ctgcgcgtca
     8221 tgatttccac cggccatctc ggcacggcgc cctcgagtcc ggaaagctac taccggggcc
     8281 tcgagaccaa tccggattac gtcgtcgcag acggcggcag ctcggatccc gggccggtgt
     8341 acctgggcga ggacatcaat ctcggccagt tcgcgcgcga ggagatggag ctcttcgtgc
     8401 cgtcgacgcg gaagaaagga attccgttaa tcatcggctc ggcaggcgac agcggctcca
     8461 atcgcggcgt cgacgagttc gtcggcatgg tgaaagagat cgcgcgcgag cagcggctgc
     8521 cgaaattcaa ggtcggctac ttctactccg aggtgccgaa ggcactcgtc aaatcaaagc
     8581 ttggaaaaag cgagggcctc ggcggcttcc ctgcgctgac cggagaggag gtggacaacg
     8641 ccacgcgcat cgtcgcggtt gccggcgcgc atccctatat gaagcttttg gacatgggcg
     8701 ctgacgtgat catcggcggc cgctccggtg acatcgcctg gaccgccgga ccgtgcatcc
     8761 gcgccggctt ccccgaaggc ctcgcgtacc acatgggcaa gatgatcgag tgcgcctcgc
     8821 tcgtcgccga gccgttcatg ggtaaggaaa cggtgatcgg cacgatcacg aaagacgaca
     8881 tccgcatcac gccgtaccac cccgaccagc gctgcaccat cgcttccgtc gccggccact
     8941 cgatgtacga gcgcgagacg ccgtacttcg agcacgcgct cggcggcacg ctcgagatgc
     9001 gcgagtgcca ctacgagcag gccgacgcgc gcaccgtgcg catcaccggg gccaaatggc
     9061 tgccggccaa ggaattaaga gtgaagctgg aaggcgcgaa gaaaatcggc gagcgctaca
     9121 tgggcttcgt cggcgtgcgc gacccgcacg tcgtgaaaaa catcgacaag gcgatcgagt
     9181 ggtcgaagac ggcggtcgcc aagcgcttcc ccgatacgaa gtacgagctc tatttccacg
     9241 tcttcggccg cgacgcgatc ctgaaggagc tcgagcccgt caaagcgcag cccgcgcacg
     9301 agctcgggct ggtggtggag ggcgtggccg ccaccgacgc gctcgccgag aagatcaccg
     9361 atttcgcgac ccgcatgttc ttcctctccc gcatcccggg agtgaaaggc accgccggaa
     9421 ccgccgccac gaccaagaag acgatgaagt catcgccggg ctacatgtgg aacgtgaacc
     9481 acaccatccc gatcgacgac ccgctggagc ttttcccctt gcacatgacg gaggccggcg
     9541 tatgaggctc tacgacctcg catcgacgat ccgtagcaag aacgccgggg tgaaccagat
     9601 caccttcgac atcatctttc ccgaccgcaa gacctacgag cgcgcggtgc gctcaggcgc
     9661 catcacgcgc gactcgatcg cgaagctgta caagatcccg gagtcgcgca tctccgattt
     9721 cgtgaccttc gatgtcggca acgcgatcaa gttcaccatc taccgcctgc gcccgagcgg
     9781 cagcccgggc gactgggaca tccttggctg ccagcagtac gggccgctac tcgacctcga
     9841 aattccctag ccgcagacgc caacgtagcc gcaggcggtg cagtaatcgc agccgtcctt
     9901 gcggatgagg gtcgggttgg cgcactcggg gcatttggcg ccggacatca ccggagccgt
     9961 cgccggtgcg gcgggcgctt caaggatgcc catctgcttc ttcgggcggc cgtcttcgtc
    10021 gaggatgccg agcatcgcgt agcggtgcag gatcaagcgc gcgacgtagg agacggtcga
    10081 agggaagttc tgctgcttgc ggcttcccgg cacgaaggcc atgaagtcgc cgagcggctc
    10141 gggaaactcc agaagcttct tcagcttcat cccgacccag gccgggtcga gcacgcgcat
    10201 gtcgagcgaa aggatcttgc acaggccatc gagcgcgcgt gggtagaccc cggaaagcca
    10261 cacggagtat ggacgcgtga tgccgtcggg cagggtgatt tcctttaggc ccagcacgaa
    10321 gtcgtcgccg gtgcgcgggt tgaagacgtc gaccgtccag ctcatcgtgc cgtcggtgcc
    10381 ggtcttcggc tccttcagcg cgaacatggc gtcgagcacc ggtgaggtcg cctgctcagc
    10441 cttcaactgg aaggtttctt cagccaagcc caggttcaac tgctcgacgc gccagcgcac
    10501 gatttgggcg aatgccgaga cgacggaggg catcagcttc ttgtcgccgc gcggcgggaa
    10561 ggccatctcg aaaggctcgt cgccgccggc cttggcgagg gtctcgagct tgagcttcag
    10621 ccagtcgctg tcgttggcgc gcatgtccat cgaaagcgtc ttggcgagcg cgccgaggcc
    10681 gcgcggctgc tcgctgccat tgacccagac ctcgaacgca tgcgggcgcc cattctcgac
    10741 atggcccacg aacaccgcga agcggtattg cgggtgctcg atcatgtagg tccacgccgg
    10801 gttgcccccg gagagatccg ggcggccggg ccagcgcagc gacgcgagca ccggcgcggg
    10861 cacggacttc accgagatgc ggcggttgac gtcgtcttgg gcgaagtcct gcggcgccgt
    10921 gacgctcagc acggaaccga gaaccttgtt cggccggtag gtggcgaggc ctttcaggcc
    10981 cgacttccac gcgtcgaaat agaggtcctg gaactccgag tacggatagt cctcgggaac
    11041 gttgaccgtc ttggagatgc tcgtgtcgat gaacggcgcg accgcggcga ccatctgctg
    11101 gtgcgccttg gcgctcatct cgagcgcggt gacgaagtag ggcgggagcg cctctacatc
    11161 gccgcccatg gcgcgatagc gccggtgggc gtggtcctca acggtgtatt cctgccagcc
    11221 gctatctacg cgcttgcggc ggatgtaggt ccacgagaag ggcggctcga taccgttcga
    11281 ggcattatcg gcgaaggcga ggctgatcgt gcccgtcggc gcgatggaga gaaggtgcga
    11341 gttgcggatg ccgttcgagc ggatgagctt cttgatctcc tccggcaggc ggccggcgaa
    11401 gccgggaccc gacaggtaaa gatccgcgtt gaagagcggg aacgcgccgc gctctttcgc
    11461 aagctcgccg gaggcccggt aggcgtggtc tcgcatcgcc tcggagatgc gcgccgccat
    11521 ggcgcgcgcc tcggcggtgt cgtactttag cgcgagcatg atgagcgcat cgccgaggcc
    11581 ggtgaagccg aggcccacgc ggcgcttcgc catcgcttcc ttgtgctgct gctcaagcgg
    11641 ccacgcggtc acttcgagca cgttgtcgag catgcggatc gccagggcga ccactttgcc
    11701 gaagcgctcg aagtcgaaag acgcattctc cgaaaattcg ttgcgcacga acttcgtgag
    11761 gtcgatggag cccaggcagc agcagccgta cggcggcagc ggctgctcgg cacacgggtt
    11821 cgtcgcctcg atcgactcgc agtagcgcag gttgttgtcg atgttgatgc ggtcgagaaa
    11881 caggatcccc ggctcggcgt gatcgtaggt cgagcgcatg atctcgtccc acagctcgcg
    11941 cgcgcgcacc ttgcggtaca cccacaggcc gtcctcgcgc tggaaagcgc cggccgactt
    12001 catgtcggtg ttcggttcgg ccttgtgcac cagctccacg tcggtgtcat cttccaccgc
    12061 ctgcatgaag gcgtcggtga cgccgatgga gatgttgaag ttggtcaggt cgccgtggtc
    12121 cttgg
//
LOCUS       DASTPD010000227        12226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_938328, whole genome shotgun sequence.
ACCESSION   DASTPD010000227 DASTPD010000000
VERSION     DASTPD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938328"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFR57_07495"
     CDS             <1..241
                     /locus_tag="VFR57_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0259242.1"
                     /translation="AIRFEDMTEEESAPLLDFLNRHSQRPEFQIRFRWRKGSLALWDN
                     RSTQHIAVNDYAGYRRVMDRVTIAGDRPYFRGTHA"
     gene            234..1190
                     /locus_tag="VFR57_07500"
     CDS             234..1190
                     /locus_tag="VFR57_07500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259243.1"
                     /translation="MPKKSFLAALLFLPLLAFAQAWPAKPVKLIAPYPPGGQTDLVSR
                     WLGEKLAPTLGQPIIVENRAGAQGAVGLQAAKAAGPDGHAFVYTNVSNLSIAPNLADN
                     LPYDAQRDFTPVTQLGLTVLAMVVPTGLNVRTLKDFVEYAKANPRKMSFASFGNGSTS
                     HVYGEMLKAAAGIEMTHVPYKGAGPAVQDIMAGHASTGIHDLGSVSPHIASGRLVALA
                     VTGPTRWPLLPDVPTFVELGYALDIAGWNAIHAPAGTPRAIVERMSTEINRVIRSPEG
                     REQMLKFGLLATGTTPEELGAVIRRDTPRWGEVIRKAGIKAD"
     gene            complement(1195..1806)
                     /locus_tag="VFR57_07505"
     CDS             complement(1195..1806)
                     /locus_tag="VFR57_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259244.1"
                     /translation="MRTVTVAVAIATSVTFVQSMAQEKPKAGSESAVVTSPGKGAAAR
                     LTTITASVEAVDAANRTVTLKGPRGNVHTLPIGPQVKNFDKIKVGDRVAVRYYEALSL
                     ELKKGGSGIRERSEREGTAQAKPGEQPAAGAARQVTAVADVVAIDAKRQVVTLRGPKR
                     TVELKVRDPKQLELVKVGDQVEATYTEALAVSVEPAPPAKQKQ"
     gene            complement(1900..2259)
                     /locus_tag="VFR57_07510"
     CDS             complement(1900..2259)
                     /locus_tag="VFR57_07510"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HEU0259245.1"
                     /translation="MTNMGIATPQLTTDLAKNLGVTEAQATGGVGAMLQQATEKLSAA
                     DFDTVRKSVPGIDQYLQASQQSLGGAKLSDIGGLPGAFKKLGLKPEMVAQFKPYVLDY
                     VGKYSPSARSTLAAALP"
     gene            2714..4360
                     /locus_tag="VFR57_07515"
     CDS             2714..4360
                     /locus_tag="VFR57_07515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0259246.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGIRVTNIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSSKMRRFNNFTTGQIYESVIKKE
                     RHGDYLGGTVQVIPHITDEIKNFIKRGAGNADVAIVEVGGTVGDIESLPFLEAIRQMG
                     LDLGRNNVCYIHLTLVPYIQSAGEIKTKPTQHSVKELREIGIQADAVLCRTIQPLPED
                     ERRKIALFTNVQKEAVISAWDVDHIYKIPMMLHEQMLDEIVCHKLQILARAADLSPWK
                     KLVHALEHPEHEVDIAFVGKYVDLQDSYKSLNEALRHAGIHTSSKVNIHYLDSEEFEK
                     GASPVPLEKMDAILVPGGFGKRGTEGKMKAIRYARENGVPYLGICLGMQLATIEFARN
                     VCGLAGANSTEFDADTPHPVVALITEWLDRTGRIEKRTEKSDLGGTMRLGSQKCPVEP
                     GTLAASIYGAEVNERHRHRYEVNNIYVPQLEAKGYKVAARTPSENLPEIMELPGHPFF
                     IGVQFHPEFTSTPRYGHPLFKAFVSAALRHRASSKLRAVA"
     gene            4357..5199
                     /gene="kdsA"
                     /locus_tag="VFR57_07520"
     CDS             4357..5199
                     /gene="kdsA"
                     /locus_tag="VFR57_07520"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU0259247.1"
                     /translation="MKLCGFEVGLDRPLFLIAGPDTLESEQLCLDVAGKLKEHTAQLG
                     MPYVFKGSFDKANRTSGQSYRGPGMEEGLRILSAVKQRIGVPVLTDVHEDTPIKDVAA
                     VVDVIQTPAFLCRQTNFIRSAAAAGKPVNIKKGQFLSPWEMKNVVEKAASTGNRQLLV
                     CERGFSFGYNNLVVDMRGLAAMRDTGYPVVFDATHSVQLPGGQGSASGGQREFISVLA
                     RAAVAAGVSGVFMETHPKPDEALCDGPNSWPLALMPELLDTLADIDRLVKKRGFVEAK
                     VKSK"
     gene            5196..6479
                     /gene="eno"
                     /locus_tag="VFR57_07525"
     CDS             5196..6479
                     /gene="eno"
                     /locus_tag="VFR57_07525"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0259248.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     AREAVELRDGDKGRYGGKGVLKAVEFVNTEISEAIVGLDASEQAFVDKTLIDLDGTEN
                     KSRLGANAVLAVSMAVAKAAAEESGLPLYRYLGGAGGMRMPVPMMNVVNGGAHANNNL
                     DFQEFMIVPVGAQSFREALRCGAEIFAALKKIIDSKSMSTAVGDEGGFAPNLPNHAAA
                     IEMIVEAARNAGYTAGQDVMMALDCAASEFHSGGKYELESEKKTLGSSEFADYLAELA
                     ARYPIVSIEDGMAEDDWEGWKRLTEKLGGKIQLVGDDLFVTNTRILRQGIRQGVANAI
                     LIKVNQIGTLTETFAAIELAKRAGYGTVISHRSGETEDTTIADIAVGSNALQIKTGSL
                     SRSDRVAKYNQLLRIEEDLGDSVSYPGREAFGKLG"
     gene            6476..6772
                     /gene="ftsB"
                     /locus_tag="VFR57_07530"
     CDS             6476..6772
                     /gene="ftsB"
                     /locus_tag="VFR57_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEU0259249.1"
                     /translation="MRILGAIFVALIVLIQYPLWLGKGGWLRAWEVERQVDAQKAKNR
                     QLEARNASLAAEVRDLKTGTEAIEERARHELGMLRPDEIFYQYVPDKKPGFEPH"
     gene            complement(6769..7215)
                     /locus_tag="VFR57_07535"
     CDS             complement(6769..7215)
                     /locus_tag="VFR57_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259250.1"
                     /translation="MRLALAGLALAFIAGCSGLRAYPDTSGSKNLTIRTNASKVRAEL
                     DVHSVDPQCRAPYIGTVGLDRPTVAVGIPTDAWSYLKFDFASSSLLRGSQRVTKATLL
                     RARAGHRYEVDVKYRDDIYDVVIRELSPRGATREVPLRDLASCEKS"
     gene            complement(7212..7715)
                     /locus_tag="VFR57_07540"
     CDS             complement(7212..7715)
                     /locus_tag="VFR57_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259251.1"
                     /translation="MKRIAVVASLGAVACAPMPQTAEEFRKAVPGAVMAKADSYEVNR
                     PMKDVAATFRRKAPECLNVSVRTVSQTTTSYQNILTEYKPTVVVNGERAELHVQQLHK
                     TGVMYPSKPPEGGIYLLVADAYPLPGNRTRIQTYGPSKGYDVLYRAVRGWASGDNVGC
                     PDMTKIG"
     gene            complement(7821..8360)
                     /locus_tag="VFR57_07545"
     CDS             complement(7821..8360)
                     /locus_tag="VFR57_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0259252.1"
                     /translation="MVSRDVPVPRWPLSELGRRRMRAGLRQPWVRDITSIYCSTEQKA
                     IDGATILSEHVGVPFAEVAELGENDRSATGFLPPDEFERVADEFFARPEVSVRGWERA
                     VDAQARIVKAVERIAEKENGVIAIVAHGAVGTLLYCHLAGEPIARRWDQPSNGGGHYF
                     PFTLSPRAALSWWQPIDQE"
     gene            complement(8389..9639)
                     /locus_tag="VFR57_07550"
     CDS             complement(8389..9639)
                     /locus_tag="VFR57_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0259253.1"
                     /translation="MEWISDPAVWVGLLTLVVLEIVLGVDNLIFIAILADKLPPQQRD
                     RARVVGLSLALVMRLGLLASITWVMSLTTPILNFWRFEISWRDVILILGGVFLLIKAT
                     TEIHERLEGGHKHGKGAKHAAFWPIIGQIVVLDIVFSLDSVITAVGMVDELPVMMAAV
                     IIAVAAMLFASKPITRFITARPTLIILCLGFLLMIGLVLVVDGFGFHIPKGYVYAAIG
                     FSVLIEILNQLAARGRRQAAASGMSSRQQITDAVLRFLGGVPLPALAIPEGKVFGPEE
                     RGMVGGVLTLGEREVRSIMTPRDAVHSLDLKEQDVLAKMRGSPHREMPVLDGSPDNVI
                     GIIRKEDVLALCVEGKPIDLKSILRPARTVLLRHTVLATLNRFKRTTTELAIVVDEQG
                     RFQGVVTRTDLLEAIAGEFPDEGE"
     gene            9680..10525
                     /locus_tag="VFR57_07555"
     CDS             9680..10525
                     /locus_tag="VFR57_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0259254.1"
                     /translation="MKLTAEQIQRFDKDGYLFFPSLFTPEEIKVLTDEVPSLYAQRRP
                     ENVREKTGDVVRTNFAAHMYSYPFAKLARHPRMVEPIKQLFGEDVYMHQFKINGKAAF
                     DGDVWQWHQDYGTWRADDQMPEARAMNVAIFLDGVNEFNGPLMFIPGSHKLGVLDAEH
                     DVTTTSYPLWTINHQTIGKLVERGGIDAPKGPAGSMILFHGCLVHASTSNLSPWNRVS
                     VYLSLCAVSNHIRRFKRPGYIAHRDFTPIRSLPDDCLVKHYDVPLPWKDGTPPEELEG
                     VLKAA"
     gene            10551..11915
                     /locus_tag="VFR57_07560"
     CDS             10551..11915
                     /locus_tag="VFR57_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEU0259255.1"
                     /translation="MNLYAKLQQRKDKPLRIGLIGAGKFAAMYIAQVPKTPGVHLVGI
                     ADIAPDYAKGNLQRIGWAPEQYGAASLDAALKEKRTHLGDNWEALVAHPAIDIVIEAT
                     GNPPAAVEHALAAFRNKKHVVMVTVEADAFCGPLLAKKAAEAGVVYSLAYGDQPALIC
                     DLVDWARAAGFPVVAAGRGHKWLPHFAQSTPETVWGHYGLTPEEARVGGLNPKMFNSF
                     LDGSKPAIETTAVCNATGLTPAPGGLLYPPGSVDEIPALMRPRSEGGVLHHKGQVEVV
                     SSLTADGKAIDYDIRFGVFVVFEGETEYIKRCFHEYGVKTDPSGRYACMYKRWHLIGL
                     EVGISVASVGLRGEPTGCATGWRADVVAVAKKPLQAGEMLDGEGGYTVVGRLMPAADS
                     LAGGALPLGLAHGWKVLRPVPAGQPVKWSDVAVDANQTAVRVRREMEQAMSARAASVQ
                     EKVA"
     gene            complement(11912..>12226)
                     /locus_tag="VFR57_07565"
     CDS             complement(11912..>12226)
                     /locus_tag="VFR57_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEU0259256.1"
                     /translation="MVGQRHSLANFFLKHRIPSAAGWVAFAESGHLVTYGPERHAVWR
                     RLAYFVDRIIKGTRPGALPVELPSVMELAVNRRTATAMGLSLPQGVLFRADRVIDTAP
                     PV"
BASE COUNT         2282 a         3915 c         3943 g         2086 t
ORIGIN      
        1 cgcgatccgc ttcgaggaca tgacagagga agaatccgcg ccgctgctcg atttcttgaa
       61 ccgccacagc cagcgccccg aattccagat ccgctttcgc tggcgcaagg gctcgctcgc
      121 gctctgggac aaccgctcga cgcagcacat cgcggtcaac gattacgcag gctaccgccg
      181 cgtgatggac cgtgtcacga tcgccggaga ccggccgtat ttcaggggga ctcatgccta
      241 agaagagctt tctcgccgcg ctcctgtttc tccccttgct cgcattcgcg caggcgtggc
      301 cggcgaagcc ggtgaagctg atcgcgccgt atccgccggg gggccagacc gacctggtgt
      361 cgcgctggct cggcgagaag ctcgctccaa ctttgggtca gccgatcatc gtggagaacc
      421 gcgccggcgc gcagggcgcg gtcggtcttc aggccgccaa ggcggcggga ccggatggcc
      481 atgcgttcgt gtacaccaat gtgtcgaatc tctcgatcgc gccgaacctc gccgacaacc
      541 tgccgtacga cgcgcagcgc gacttcacgc cggtcacaca gctcgggctc accgtgctag
      601 cgatggtggt gccgactgga ctcaatgtgc ggacgctgaa ggacttcgtc gaatacgcca
      661 aagcgaaccc gcgaaagatg agcttcgcct cgtttggtaa tgggtcgacc tcgcacgtgt
      721 acggcgagat gttgaaggcc gcggccggca tcgagatgac ccacgtgccg tacaagggcg
      781 cgggccctgc ggtgcaggac atcatggccg ggcacgcgag cacgggcatc cacgatcttg
      841 gaagcgtcag cccgcacatc gcatccggtc ggctcgtcgc gctcgcggtc accggaccga
      901 cgcgctggcc gctcctgccg gacgtgccga ccttcgtgga gctcggctat gcgctcgaca
      961 tcgccggctg gaacgcgatc catgcgcccg ccggcacgcc gcgggcgatc gtcgagcgca
     1021 tgagcacgga gatcaaccgc gtgatccgat cgccggaggg ccgcgagcag atgctgaagt
     1081 ttgggctgct cgcgaccggc acgacgcccg aggaactcgg cgctgtgatc cgccgcgaca
     1141 cgccgcgctg gggcgaggtg attcgcaagg ccggaatcaa ggccgactga gctcctactg
     1201 tttctgtttg gcgggcggcg ccggctcgac ggacactgcc agcgcctcgg tgtaggtcgc
     1261 ctcaacctgg tcgccgactt tgacgagctc aagctgcttc gggtcacgca ccttgagctc
     1321 gacggtacgc ttcggaccgc gcaacgtaac gacctggcgc ttcgcgtcga tcgcgaccac
     1381 atcggcgacg gcggtgacct gtcgcgccgc tcccgccgca ggctgctcgc ccggcttcgc
     1441 ctgcgcggtt ccctcgcgct cgctgcgctc gcggatgccc gagccgcctt tcttgagctc
     1501 gagcgagagg gcctcgtaat agcgcacggc gacgcgatcg ccgaccttga tcttgtcgaa
     1561 gttcttcacc tgcggtccga tcggcagcgt gtgtacgttg ccgcgcggtc ccttcaaggt
     1621 gacggtacgg ttcgcagcgt cgacagcctc gacggaggcc gtgatcgtcg tgagcctcgc
     1681 tgccgcgcct ttgcccggcg aagtgacgac ggctgactcc gagcccgcct tcggcttctc
     1741 ttgcgccatg gattgcacga aggtgacgct cgtggcgatt gcgacggcaa cggtgacggt
     1801 acgcatgagg cgttctcctt aggtggacac gggccccaac gtagcgcggc gcgtgcgcgc
     1861 gcaccatcgg accaaggttc atcggcgcgg cgagttcgtc tagggcaggg cggcggcgag
     1921 tgtactcctc gcgctggggc tgtacttgcc cacgtagtcc agcacgtacg gcttgaactg
     1981 ggcaaccatt tccggcttga gccccagctt tttgaacgca cccggcagcc cgccgatatc
     2041 ggacagcttc gcacccccga gtgattgctg cgacgcctgc aagtactggt cgatgccggg
     2101 cacggatttc ctcacggtat cgaaatcggc cgcactcagc ttctcggtgg cctgctgaag
     2161 catcgcgccg accccgccgg tcgcttgtgc ctccgtaacg ccgaggttct tggcgagatc
     2221 cgtcgtgagc tgcggcgttg cgattcccat gttagtcatt tgcttctttg tggtttcaca
     2281 gccggagagc aacgcgacta acagcatgag catggagcag gctaatgggc gatacatagg
     2341 acctccttta gaggcgtgag aattgtagga gaggagcatg cgggctttgt tcgcgatgga
     2401 ctgatgttcc acgtgtatcg cggaggtcta ttatattttt gtgtggcgcc gcttgtactg
     2461 cggcgctcta ctcacgcgcc ccggcggtgg acgatggcgc agggtgctcg ttgttggctg
     2521 atgccattgg gcaccggggt cctcgcccgg ggcagagccg gcgttggaaa aggatgcagc
     2581 cctaaggcgc tcggcggcaa ttccgggtac catggcggtc catggttcat agccatggag
     2641 gcaacgccac cggtcgtacg ccgtgcacta caaacccggc cttctcccta caggtattgc
     2701 ccgagcccgg tagatgacca agtacgtttt cgtaaccggc ggcgtagttt cctctcttgg
     2761 gaagggcatc gccgccgcgt cccttgccgc aatcctcgaa tcccgcggca tccgcgtcac
     2821 caatatcaag ctggacccgt acatcaacgt cgaccccgga acgatgtcgc cgttccagca
     2881 cggcgaagtg ttcgtgaccg aggatggcgc cgagaccgac ctggacttgg gccactacga
     2941 gcgcttccag agctcgaaga tgcgccgctt caataacttc accaccggcc agatctacga
     3001 gtcggtgatc aagaaggagc ggcatggcga ttacctcggc ggcacggtgc aggtcatccc
     3061 gcatatcacc gacgagatca agaacttcat caagcgcggc gccggcaatg cggacgtcgc
     3121 gatcgtggaa gtgggtggaa cggtgggcga catcgagtca ctgcccttcc tcgaggcgat
     3181 ccggcagatg ggtttggatc tcgggcggaa taacgtctgc tacattcact tgaccctcgt
     3241 gccgtacatc cagtcggccg gcgagatcaa gaccaaaccc acccagcact cggtgaagga
     3301 gctgcgcgag atcggcatcc aggccgacgc ggtcctgtgc cgcacgatcc agccgctgcc
     3361 ggaagatgaa agacgcaaga ttgcgctctt cacgaacgtt cagaaggagg cggtgatctc
     3421 cgcctgggac gtcgaccata tctacaagat ccccatgatg ctgcacgagc agatgctcga
     3481 cgagatcgtc tgccacaagc tgcagatcct ggcgcgcgca gcggatctct cgccctggaa
     3541 gaagctggtg cacgccctcg agcaccccga gcacgaggtc gacatcgcgt ttgtcggcaa
     3601 gtatgtcgac ctgcaggatt cctacaagtc gctgaacgaa gcactgcggc acgcgggcat
     3661 ccacaccagc tccaaggtca acattcacta cctcgactcc gaggagttcg agaagggcgc
     3721 gagccctgtg cctctggaga agatggatgc gatcctcgtg ccgggggggt tcggcaagcg
     3781 cggcaccgaa ggcaagatga aggcgatccg ctatgcgcgc gagaacggcg tgccgtatct
     3841 cggcatctgc ctcggcatgc agcttgcgac catcgagttc gcgcgcaatg tgtgcggcct
     3901 ggccggcgct aattccaccg agttcgacgc cgacacgccg catcccgtcg tcgcgctcat
     3961 taccgagtgg ctcgatcgca ccgggcgcat cgagaagcgc accgagaagt ccgatctggg
     4021 cggcaccatg cgcttaggca gccaaaagtg tcctgtcgag cccggcacgc tcgccgcatc
     4081 gatctacggc gcggaagtga acgagcgcca tcgccaccgg tacgaggtca acaacatcta
     4141 cgtcccgcag ctcgaggcga agggctacaa ggtggccgcg cgcacaccga gcgagaacct
     4201 gccggagatc atggagctcc ccgggcaccc gttcttcatc ggggtgcagt tccatccgga
     4261 gttcacctcg acgccgcgct acggtcaccc gcttttcaag gcattcgtct ccgcggccct
     4321 gcgccaccgg gccagctcca agctccgggc cgtagcgtga agctttgcgg cttcgaagtc
     4381 ggcctcgacc ggccgttatt cctcattgcc gggcccgata cgctggaaag cgagcaactc
     4441 tgcctcgacg tcgccggcaa gctgaaggag cacaccgcgc agctcggcat gccctacgtc
     4501 ttcaagggct ccttcgacaa ggcgaaccgc acgtcgggcc agagctatcg cggcccggga
     4561 atggaagaag gcctgcgcat cctttcggcg gtgaagcaac gcatcggggt gccggtgctc
     4621 accgacgtgc acgaagacac gccgatcaag gacgtcgcgg ccgtcgtcga cgtgatccag
     4681 acgcccgcgt tcctctgtcg tcagaccaac ttcatcagga gcgcggccgc ggccggcaag
     4741 ccggtgaata tcaagaaagg acagttcctc tcgccctggg agatgaagaa cgtcgtggag
     4801 aaggcggcga gcaccggcaa ccggcagctg ctcgtgtgcg agcgcggctt cagcttcggt
     4861 tacaacaatc tcgtggttga catgcgcgga ctggcagcga tgcgcgacac cggctatccg
     4921 gtcgtctttg acgccacgca ttcggtccag cttccgggcg ggcagggcag cgcctccggc
     4981 ggccaacgtg aattcatctc cgtgcttgcg cgcgccgcgg tcgcggcggg agtttcgggc
     5041 gtcttcatgg agacgcatcc gaaacccgac gaggcgctgt gcgacggccc gaactcgtgg
     5101 ccgctcgcgc tcatgcccga attgctcgat acgcttgcgg acatcgaccg actggtcaag
     5161 aaaagaggat tcgtggaagc gaaggtcaaa tcgaaatgag ctccatcgtc gacgtggtgg
     5221 caagggagat cctcgactcg cgcggcaacc cgacggtcga ggccgacgtg ctgctcgaat
     5281 ccggcgtgat gggacgcgcg gccgtccctt ccggcgcatc caccggcgcg cgtgaggccg
     5341 tcgaattgcg cgatggcgac aaaggccgct atggcggcaa gggtgtgctt aaagcggtgg
     5401 agttcgtgaa caccgaaata tcggaggcca tcgtcggact cgacgcgagc gagcaggcct
     5461 tcgtcgacaa gacactcatc gatctcgacg gcaccgagaa caagtcgcgc ctcggcgcga
     5521 acgccgtgct ggcggtgtcg atggccgtgg cgaaagccgc ggccgaagaa tccggactgc
     5581 cgctctatcg ctacctcggc ggcgccggcg gcatgcgcat gccggtgccg atgatgaacg
     5641 tggtgaacgg cggcgcgcac gcgaacaaca acctcgactt ccaggaattc atgatcgtcc
     5701 cggtgggcgc gcagtccttc cgcgaggcgc tgcgctgcgg cgccgagatt ttcgccgcgc
     5761 tcaagaagat catcgactcg aaaagcatgt ccaccgcggt gggcgacgag ggcggctttg
     5821 cgccgaacct gccgaaccac gccgcggcga tcgagatgat cgtcgaagcg gcgcggaacg
     5881 ccggctatac ggccggccag gacgtgatga tggcgctcga ctgcgctgct tctgaattcc
     5941 acagcggcgg gaaatacgaa cttgagtccg agaagaagac gctcggctcg tccgagttcg
     6001 ccgactatct cgccgagctc gccgcgcgct acccgatcgt gtcgatcgaa gacggcatgg
     6061 ccgaggacga ctgggaaggt tggaagcgcc tgaccgagaa gcttgggggc aagatccagc
     6121 tcgtgggcga cgacctcttc gtcaccaaca cgcgcatcct gcgccagggc atccgccagg
     6181 gcgtagccaa cgcgatcctc atcaaggtga accagatcgg cacgctcact gaaaccttcg
     6241 ccgcgatcga gctcgccaag cgcgccggct acggcaccgt gatctcgcac cgctccggcg
     6301 agacggaaga caccaccatc gccgacatcg ccgtcggttc caacgcgctt cagatcaaga
     6361 ccggatcgct gtcgcgctcg gaccgcgtcg cgaagtacaa ccagctcctg cgcatcgagg
     6421 aagacctcgg cgactcggtg agctatcccg ggcgggaggc gttcggcaag ctcggatgag
     6481 aatcctcggc gccatcttcg tcgccctgat cgtgcttatc cagtatccgc tatggttggg
     6541 caagggcggc tggctgcgcg cctgggaagt cgagcgtcag gtcgacgcgc agaaagcgaa
     6601 gaaccggcag ctcgaggcgc gcaacgcctc gctcgccgcc gaagtgcgtg acctgaagac
     6661 cggaaccgaa gcgatcgaag agcgcgcgcg ccacgagctc ggcatgctgc gcccggacga
     6721 gattttttac cagtacgtcc cggacaagaa accggggttc gagccccact aggacttctc
     6781 gcaggacgcg agatcgcgaa gcggaacttc gcgcgttgcg ccgcgtggcg acagttcgcg
     6841 aatcacgacg tcgtagatat cgtcgcgata tttgacgtca acttcgtagc gatgaccggc
     6901 gcgagctcga agcaaggtcg ccttggtcac tcgctgcgag ccgcggagaa ggctggagct
     6961 ggcgaagtcg aacttgaggt agctccatgc atcggtcggg atgccgacag caacggtcgg
     7021 cctgtccagc cctacggtgc cgatgtacgg ggcgcggcac tggggatcga cgctgtggac
     7081 gtcgagctcg gcacgtacct tcgacgcgtt cgtcctaatg gtcaggttct tggaaccgct
     7141 tgtatccggg tacgcccgca atccgctgca gcctgcgata aacgccagcg cgagcccggc
     7201 gagcgcgagc ctcatccgat cttcgtcatg tccgggcaac cgacattatc gccgcttgcc
     7261 catccacgca ccgcgcgata cagcacgtcg tagcccttcg acggtccata ggtctggatc
     7321 cgcgtgcggt taccgggcag aggatacgcg tcggccacga gcaggtagat gccgccctcg
     7381 ggtggtttgc tcggatacat gacgccggtt ttatgcagct gctgcacatg gagctcggca
     7441 cgctcgccgt tcaccacgac cgtgggttta tattcggtaa gaatgttctg gtaactggtg
     7501 gtcgtttgcg agacggtgcg caccgagacg ttcaggcact ccggcgcctt gcggcggaag
     7561 gtggccgcca cgtccttcat gggacggttc acttcatagc tgtcggcctt cgccatcacc
     7621 gcgcccggca ccgccttgcg gaactcctcg gcggtctgcg gcatcggcgc gcaagccacg
     7681 gcgccgagtg aagccactac ggcgattctt ttcataagcc cccctgttct ttgttattgg
     7741 tcgatcgctt gccattgcac tgtagcgcag ccggcaagag gggaaggcgg aagtaattcg
     7801 cgcctcgttg ctcgccgatc ctactcttgg tcgatcggct gccaccacga tagcgcggca
     7861 cgtggcgata gcgtgaaagg aaagtaatgg ccgccgccgt tggacggctg atcccagcgt
     7921 cgcgcgatcg gctcgccggc gagatggcag tagagcaggg taccgaccgc gccgtgcgcc
     7981 acgatggcaa tcacgccgtt ctccttctcg gcgatgcgct cgaccgcttt cacgatgcgc
     8041 gcctgcgcat cgacggcgcg ctcccagccg cgcacggaaa cctccggacg cgcgaaaaac
     8101 tcgtcggcga cgcgctcgaa ctcatccggc ggaagaaagc cggtcgccga acggtcgttc
     8161 tcgcccagct cggccacttc cgcaaatggg acgcccacgt gctcggaaag aatggttgcg
     8221 ccatcgatcg ccttctgctc ggtgctgcag tagatcgacg tgatgtcgcg cacccatggc
     8281 tgccgcaagc cagcgcgcat gcggcgccta ccgagctcgg agagcggcca ccggggcacc
     8341 ggcacgtcgc ggctcaccac gacgttcgga tgcgtgatga agtaaaagct actcgccttc
     8401 gtcgggaaac tcgccggcga tggcttcgag aaggtcggtg cgcgtcacga cgccctggaa
     8461 acgtccttgt tcgtcaacca caatcgcgag ctcggtcgta gtgcgcttga agcggttcag
     8521 cgtcgcgagc accgtatggc ggagcaatac ggtgcgcgcg gggcgcagga tgcttttgag
     8581 gtcgatcggc ttgccttcga cgcagagcgc gagcacgtcc tctttgcgga tgatgccaat
     8641 cacattgtcc ggcgagccat cgagcaccgg catctcgcga tgcgggcttc cgcgcatttt
     8701 cgcgaggacg tcctgctcct tgaggtccag cgagtggacg gcgtcgcgcg gcgtcatgat
     8761 ggagcgcacc tcgcgctcgc ccagagtaag gaccccgccg accatgccgc gctcctcggg
     8821 gccgaatacc ttgccttccg ggatggcgag ggccggaagc ggcacgccgc cgaggaacct
     8881 gagcacagcg tcggtaatct gctggcgcga actcatgccg gacgccgcgg cctggcgccg
     8941 cccgcgcgcg gcgagctgat tcagaatctc gatcagcacc gagaacccga tcgcggcata
     9001 cacatagccc ttcgggatgt ggaagccgaa gccgtcgacc acgagcacca ggccgatcat
     9061 caggaggaac ccgaggcaca ggatgatcag tgtcgggcgc gcggtgatga accgcgtgat
     9121 cggcttggag gcaaagagca tcgccgcgac ggcgatgatc accgcggcca tcatgacggg
     9181 cagctcgtcc accatgccga cggccgtgat gaccgaatcg agcgagaaca cgatgtcgag
     9241 caccacgatc tgcccgatga tcggccagaa ggctgcatgc ttggccccct tgccgtgctt
     9301 gtggccgccc tcgagccttt cgtggatctc ggtggtcgcc ttgatgagga ggaacacgcc
     9361 gccgaggatc aggatcacgt cgcgccaaga gatctcgaaa cgccagaagt tcaggatggg
     9421 cgtggtgagg gacatcaccc atgtgatgct cgcaagcagc cccagacgca tgacgagcgc
     9481 gagcgagagc ccgaccaccc gggcgcggtc gcgctgctgc ggcggcagct tgtcggccag
     9541 gattgctatg aaaatcaggt tgtcaacgcc gaggacgatc tccaaaacga cgagcgtgag
     9601 aagcccgacc cagacggccg gatcagagat ccattccatg gattacctcg gtggagtagt
     9661 cggcgcttat actatttgaa tgaaactgac cgcggaacaa atccagcgat tcgacaagga
     9721 cggatatctc ttcttcccga gccttttcac cccggaagag atcaaggtcc tgaccgacga
     9781 agtgccttcg ctctatgcgc agcggcggcc ggagaacgtg cgcgaaaaga ccggcgacgt
     9841 cgtgcgcacg aacttcgccg cacacatgta cagctatccg ttcgccaagc tcgcgcgcca
     9901 tccgcgcatg gtcgagccga tcaagcagct tttcggcgaa gacgtgtaca tgcaccagtt
     9961 caagatcaac ggcaaggctg cgttcgacgg cgacgtctgg caatggcatc aggactacgg
    10021 cacctggcgc gccgacgacc agatgcccga ggcgcgcgcg atgaacgtcg cgatcttcct
    10081 cgatggagtg aacgagttca acggtccgct catgttcatt ccgggcagcc acaagctcgg
    10141 cgtactggac gccgaacacg acgtgaccac gacgagctat ccgctctgga ccatcaacca
    10201 ccagacgatc ggcaagctgg tcgagcgcgg cggaattgac gcgccgaaag gtccggcggg
    10261 ctccatgatc cttttccacg gctgcctggt gcatgcctcg acctcgaatc tctcgccgtg
    10321 gaaccgcgtg agcgtgtatc tctcactctg cgccgtttcg aatcacatcc gccgctttaa
    10381 gcgtcccggc tacatcgcgc atcgcgactt cacgccgatc cgctccctgc ccgacgactg
    10441 cctcgtcaag cactacgacg tgccgctgcc ctggaaggac ggcacaccgc ccgaggagct
    10501 cgagggcgtt ttgaaagcgg cttagggaca gacccgggat agacccttct atgaacctct
    10561 atgcgaagct ccagcagcgc aaggacaagc cgctgcgcat cggcctgatc ggcgcgggca
    10621 agttcgccgc gatgtacatc gcgcaggtgc cgaaaacccc aggcgtgcat ctcgtcggaa
    10681 tcgccgacat tgcgccggat tacgcgaagg gcaacctcca gcgcataggc tgggcgcccg
    10741 agcagtacgg tgcggcctcg ctcgacgctg cgctcaagga aaagagaacg cacctcggcg
    10801 acaactggga agccctggtc gcgcatccgg cgatcgacat cgtgatcgaa gccaccggaa
    10861 atccgcccgc cgcggtggag catgcgctgg ctgctttcag gaacaagaag catgtcgtca
    10921 tggtgacggt ggaagccgat gcgttctgcg ggccactgct cgcgaagaag gcggccgaag
    10981 ccggcgtggt gtacagcctt gcctatggcg accagccggc gctgatctgc gacctcgtgg
    11041 actgggcgcg cgcggctggg tttccggtcg tcgccgcggg caggggacac aagtggctgc
    11101 cgcatttcgc gcagtccaca ccggagaccg tctggggtca ttacgggctg acgccggaag
    11161 aggcccgggt cggcgggctg aaccccaaga tgttcaattc gttcctcgac gggtcgaagc
    11221 ccgcaatcga gaccacggcg gtgtgcaacg caacgggttt gacaccggca ccgggcggac
    11281 tgctctatcc ccccggttcc gtcgatgaaa taccggcgct catgcgtccg cgaagcgaag
    11341 gcggagtcct gcatcacaag ggacaggtcg aggtcgtctc ctcgcttacc gcggacggaa
    11401 aggccatcga ctacgacatc cgcttcggcg tgttcgtggt gttcgaaggc gagacggaat
    11461 acatcaagcg ctgcttccac gagtacgggg tgaagaccga tccttcggga cgctatgcgt
    11521 gcatgtacaa acgctggcac ttgatcggcc tcgaagtcgg catctctgtc gcctccgtgg
    11581 gactgcgcgg cgagccgacc ggatgcgcga ccgggtggcg cgccgatgtc gtcgccgtgg
    11641 ccaagaaacc cctgcaggcc ggagaaatgc tcgacggtga gggcggctac acagtcgtgg
    11701 gcaggctcat gcccgcggcg gattcacttg cgggcggggc attgccactg ggtttggcgc
    11761 acggctggaa ggtgctgcgc cccgttcctg cgggccagcc ggtgaagtgg agcgacgtcg
    11821 ccgtcgacgc gaaccagacc gcggtgcgcg tgcggcgcga gatggagcag gcgatgagcg
    11881 ctcgcgcagc ttcggtacaa gaaaaagtcg cctagaccgg cggcgcagta tcaatcacgc
    11941 ggtcggcgcg aaacagcacg ccttgcggca gtgaaagacc catggccgtg gccgtgcgcc
    12001 gattcacggc gagctccatg acgctgggca gctcgaccgg cagggcgccg gggcgcgtgc
    12061 ccttaatgat ccggtcgaca aaataggcca accgccgcca cacggcgtgc cgttccggcc
    12121 cgtacgtcac aaggtgaccg ctctccgcga aggccaccca accggcggcg cttggaatgc
    12181 ggtgtttcag aaagaagttg gcgagcgagt ggcgctgtcc gaccat
//
LOCUS       DASTPD010000228         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_735686, whole genome shotgun sequence.
ACCESSION   DASTPD010000228 DASTPD010000000
VERSION     DASTPD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735686"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..722
                     /locus_tag="VFR57_07570"
     CDS             294..722
                     /locus_tag="VFR57_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259257.1"
                     /translation="MGSRHRILVAAQPAAWPVLKNLLEELADLVPVHGMTEAMQVLEQ
                     DPVGIDLIICTLTFSDHRMLEFLLQVKGNPKTSAIPFLVCRTLVGRLSEELVVRMGAL
                     ARQFEADFINLPRFPADEARKELSAAVTKCFASNRRRPKR"
     gene            complement(772..1389)
                     /locus_tag="VFR57_07575"
     CDS             complement(772..1389)
                     /locus_tag="VFR57_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259258.1"
                     /translation="MFLILLGGALALGASLFVAASFTAGSFVSVRTVAAIIGWLFVLA
                     TLGCLLGLRQSSFARLTAGAVTFCSGLLLVYVAHFEWTAIQPMPAAYAAAKPLHADPK
                     LIQAPALKLAAALGMSETAAWERAKTAPPTKPIYAVASASQPRAQPAPCAALAGIESL
                     QCLRCPEKSGVALIVCRESARLEFCEGQHFADATCPSPYPHSYPG"
     gene            1464..2429
                     /locus_tag="VFR57_07580"
     CDS             1464..2429
                     /locus_tag="VFR57_07580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEU0259259.1"
                     /translation="MRRFLFVLAFVSAAALAQDKAAETLNAVVGISAKIQKDARSAET
                     LGLQRSGSGAIVRDGYVLTIGYLVIEAEAVQVTGADGRTLPATVAAYDHASGFALLKV
                     IGSMPAKPLALGDSAALGERDPAMAVSASSRDAPAIVYVVSRRAFSGNWEYLLDSAIF
                     TYPPVMDWSGAPLIGASGELLGIGSLIVPDAGAPGTQSPGNMFVPVDVLKPILEDLIA
                     RGRRNGPARPWLGLNADEVRGRLFVGRVSPDGPAARAGLTSGDILLAVGNEEVSTLAD
                     FYRKLWGRGPAGTEVPLKVLKGARVVEVKVRSIDRVEYFRPSRTY"
     gene            2506..2727
                     /locus_tag="VFR57_07585"
     CDS             2506..2727
                     /locus_tag="VFR57_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEU0259260.1"
                     /translation="MIDTPPTEEEKVQIRVRLQALEVEHHDLDDVIGRLVVDPTQDRL
                     QLQRLKKRKLLLKDQIQRLRARLIPDIIA"
     gene            2724..3179
                     /gene="dtd"
                     /locus_tag="VFR57_07590"
     CDS             2724..3179
                     /gene="dtd"
                     /locus_tag="VFR57_07590"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU0259261.1"
                     /translation="MIALIQRVAEARVAVGGETVGAIGAGLLVLLGVERDDREAEAAR
                     LAERVLGYRVFADSAGKMNLSVKDTSGGLLVVPQFTLVADTNSGTRPSFSSGASPQEG
                     ERLYGRFVEETRTRHARVETGRFAAHMMVSLVNDGPVTFWLQVSPKSRS"
     gene            complement(3115..3549)
                     /locus_tag="VFR57_07595"
     CDS             complement(3115..3549)
                     /locus_tag="VFR57_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0259262.1"
                     /translation="MPAKAYPKSFSVSHLRGSRFERRGLRSYFEYRDLGIERATRGRV
                     VAHVIRARPGKAPHGEWHAHQCNVQFVYVLKGWVLFEYEGVGRVMMKAGSCFYQPPNI
                     RHRELRHSKDIEMLEIVAPAQFKTYSLEKPAARKSPARRSRG"
     gene            3548..>4357
                     /locus_tag="VFR57_07600"
     CDS             3548..>4357
                     /locus_tag="VFR57_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU0259263.1"
                     /translation="MFEQFIGTKPVEERHRFDVSSLEHYLAQHIPELRGPLAVEQFKG
                     GQSNPTYKVSDAAGRRFVLRRKPPGKLLPSAHAVDREFRVIHALYPTGFPVAKPHVLC
                     EDALVIGTPFYVMDFVDGRVLWDQSLPGMTKAERAAIWDELNRVIAQLHTIDYRTVGL
                     EDFGKPGNYIERQISRWTKQYQASETERVEAMDNLIAWLPKNIPPSASTTVVHGDYRL
                     DNAIFHPQEPRILAVLDWELCTLGDPLADFAYHCMSWHIPPGQFRGIAGLDL"
BASE COUNT          781 a         1373 c         1440 g          763 t
ORIGIN      
        1 agttaactac ttaggcgata tgcgatctct ttacttcaat ggataggtta gtgcattaaa
       61 ggaatagatc agctgcctta caagggcgcg ctttctccgc gcgcgtacgc acgctagacc
      121 gtttgcatga ctttagtttg accatggcgg gggctaatcc ccgcaacaac cgggcaaaag
      181 gcgggtacat tgggcttaat accgacatgc atggtcacca ggagtgcgct gaatagcctg
      241 gccttcctag aaaaagccag cgccgacggg tataaacgaa gggaaggcgc ggtatgggaa
      301 gcaggcatcg gatattggtt gcagcgcagc cggccgcctg gccggtattg aagaacctgc
      361 tcgaagagct cgccgacctg gtgcccgtac acggcatgac cgaggcgatg caggtgctgg
      421 agcaggatcc ggtcgggatc gatctcatta tctgcacact taccttcagc gatcatcgca
      481 tgctggagtt cctgctacag gtgaagggca atccgaaaac gagcgcgatt ccgtttttgg
      541 tttgccggac gctcgttgga cgcctgtccg aagagctggt cgtgcgtatg ggtgcgctgg
      601 cccggcagtt cgaggcggat ttcatcaacc tgccgcgctt cccggccgac gaggccagaa
      661 aagaattgag cgctgcggtc acgaaatgct tcgcgtcaaa taggcgaagg ccgaagcgct
      721 agccgcggcg cgcggcgcag ccgagaagcc gcatgtgcgg ctagaccgac ttcacccggg
      781 gtacgagtgt ggatacggcg atgggcaggt cgcgtcggcg aagtgctgac cttcgcagaa
      841 ctcgagccgc gcgctttccc ggcagacgat cagtgcaacg ccgctcttct ccgggcagcg
      901 caggcattgc agcgactcga ttccggcaag cgcggcgcac ggcgccggct gcgcccgcgg
      961 ttgcgatgcc gatgccaccg catagattgg cttggtcgga ggcgcggtct tcgctctctc
     1021 ccaagcggcg gtctcgctca taccgagcgc cgccgcgagc ttgagcgcgg gtgcctggat
     1081 cagtttcggg tccgcatgca gcggtttggc cgccgcgtaa gcggccggca tgggctggat
     1141 cgcggtccat tcgaaatggg ccacgtacac gagaagcagc ccggagcaga acgtgactgc
     1201 gccggccgtc agccttgcga agctcgactg tcgcagcccg agcaggcagc caagggttgc
     1261 gagcacgaag agccagccga tgatcgcggc caccgtgcgc acggacacga agcttcccgc
     1321 ggtgaaactc gccgctacga acaagctggc gccgagcgca agcgctccgc cgagaaggat
     1381 caggaacatg ggtgagccga aatattagct cattgcgcgt tcggttccgg cgggcgcatg
     1441 atcacgcaaa ccggaggccg atcatgcgtc gattcctttt tgtgcttgcc ttcgtttccg
     1501 cagccgcgct cgcccaggac aaggccgcgg aaacgctgaa cgcggtcgtc ggaatcagcg
     1561 cgaagattca aaaagatgca cgcagcgccg agacgctcgg cctgcagcgc agcggcagcg
     1621 gcgcgatcgt gcgcgacggt tacgtgctta cgatcggcta tctcgtcatc gaggcggagg
     1681 cggtccaggt aaccggggcg gatggccgca cgctgccggc taccgtggcg gcgtacgacc
     1741 acgcgagcgg tttcgcgctt cttaaagtga tcggcagcat gccggcgaag ccgcttgcgc
     1801 ttggcgactc ggcggcgctc ggcgagcggg accccgcgat ggcggtgagc gcgtcctcgc
     1861 gcgacgcgcc ggcgattgtc tatgtggtgt cgcgccgcgc gttctcgggc aactgggaat
     1921 acctgctcga ttcggcgatc ttcacctacc cgccggtcat ggactggtcg ggcgccccgc
     1981 tcatcggcgc aagcggcgag ctgctcggca tcggctcgct catcgttccg gacgccggcg
     2041 cgccgggcac ccagtcgccc ggcaacatgt tcgtaccggt cgacgtcctg aagccgattc
     2101 tcgaggacct gatcgccagg ggccggcgca acggcccggc gcggccgtgg cttggcctga
     2161 atgccgacga ggtgcgcggc cgcctctttg tcggccgggt ctcgcccgac ggtccggccg
     2221 cgcgcgcagg gctgacaagc ggggacatcc tcctcgcggt tggcaacgaa gaagtgagca
     2281 cgctggctga cttctaccga aagctttggg gccggggccc ggcaggcacc gaggtgccgc
     2341 tcaaggttct gaagggcgcg cgcgtggtgg aggtcaaggt gcgctccata gaccgggtgg
     2401 agtactttcg gccgtcgagg acctactagc cgaggcggcc ggaggactac cgccgcgcgc
     2461 gctataggga taggattggc gccatcggca tgatgctctg aaagcgtgat cgatactcct
     2521 ccgaccgaag aggaaaaggt tcagatccgc gtgcgcctcc aggcgctgga ggtcgagcac
     2581 cacgatctcg acgatgtaat tggacgcctc gtcgtcgatc ccacgcagga tcggctgcag
     2641 ctccagcggc tgaagaaacg caagctcctc ctcaaagatc agatccagcg cctgcgcgcc
     2701 aggctcatcc ccgacatcat cgcgtgatcg cgctgatcca gcgggtcgcc gaggcgcgcg
     2761 tcgccgtcgg cggcgaaacc gtgggcgcga tcggcgccgg gcttctcgtt ctcctcggtg
     2821 tcgagcgcga cgaccgcgaa gcggaagccg cgcgcctcgc cgagcgcgtg ctcggctacc
     2881 gcgtgtttgc ggacagcgcg ggcaagatga atctttcggt caaggatacg agcggcggcc
     2941 ttctcgtcgt gccgcagttc accttggtgg ccgataccaa cagcggcacg cgaccgagct
     3001 tttcttcggg cgcatcgcca caagagggcg agcgccttta tgggcggttt gtggaagaaa
     3061 ccagaacccg gcatgcgcgc gtcgaaaccg gacgcttcgc cgcgcacatg atggtcagcc
     3121 tcgtgaacga cgggccggtg actttctggc tgcaggtttc tccaaagagt aggtcttgaa
     3181 ttgcgccggc gcgacgatct cgagcatctc gatgtccttc gagtggcgga gctcgcggtg
     3241 gcggatgttg gggggctggt agaagcagct gccagctttc atcatcacgc ggccgacgcc
     3301 ttcgtactcg aatagcaccc agcccttgag cacgtagacg aactgcacgt tgcactggtg
     3361 cgcgtgccat tcgccgtgcg gcgcctttcc agggcgcgcg cggatcacgt gcgcgacgac
     3421 cctgccgcgc gtcgcgcgct cgatgccgag atcgcgatac tcgaagtagc tgcgcaggcc
     3481 gcggcgctcg aatctcgagc cgcgcaggtg cgagacgctg aagcttttgg ggtaagcttt
     3541 tgccggcatg ttcgagcagt ttatcggcac caagcccgtc gaggaacgcc accgcttcga
     3601 tgtttcatcg ctcgagcact acctcgcgca gcacattccc gagctgcgcg gtccgctcgc
     3661 ggtcgagcag ttcaagggcg gccagtccaa cccgacttac aaggtcagcg acgcggcggg
     3721 caggcgcttc gtgctgcgac gcaagccgcc gggaaagctg ctgccgtccg cgcacgccgt
     3781 cgatcgcgaa tttcgcgtga tccatgcgct ttatccgacc ggcttcccgg tcgcgaagcc
     3841 gcatgtgctg tgcgaagacg cgctcgtcat cggcacgccg ttctacgtca tggatttcgt
     3901 cgacggacgc gtgctgtggg atcagtccct tcccggcatg acgaaggccg agcgcgcggc
     3961 gatctgggat gagctgaacc gcgtcatcgc ccagcttcac accatcgact atcgcaccgt
     4021 cggcctcgag gacttcggca agccaggcaa ctacatcgag cggcagatct cgcgctggac
     4081 caagcagtac caggcctcgg agaccgagcg tgttgaagcg atggacaacc tgatcgcgtg
     4141 gctgccgaag aacattccac cgagcgcaag cacgaccgtg gtgcacggcg actatcgcct
     4201 cgacaacgca atcttccacc cccaggagcc gcgcattctc gcggtgctcg attgggagct
     4261 ctgcacgctc ggcgatccgc tcgccgactt cgcgtatcac tgcatgagct ggcacattcc
     4321 gcccggacaa ttccgcggca tcgccgggct cgatctc
//
LOCUS       DASTPD010000229         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_326111, whole genome shotgun sequence.
ACCESSION   DASTPD010000229 DASTPD010000000
VERSION     DASTPD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326111"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFR57_07605"
     CDS             complement(<1..254)
                     /locus_tag="VFR57_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259264.1"
                     /translation="MDTFLQQVVNGLTAGSLYAVVALGYTMVYGIIQLINFAHGEVVM
                     IGAMVAFSIINLLVPTGLPPFVIVLIGTGCAVPICMLVGYG"
     gene            complement(313..1494)
                     /locus_tag="VFR57_07610"
     CDS             complement(313..1494)
                     /locus_tag="VFR57_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0259265.1"
                     /translation="MKNTQIGICVIAAAVALVGCGQQSGGGGAEVRIGHVAPLTGGIA
                     HLGKDNENGARLAIEEANAAKLKIDGKDVKFTLVAEDDQADPKVGTTVAQKLVDAKVV
                     GVVGHLNSGTSIPASPIYNQAGIPVISGSATNPKLTEQGFKTQFRVVARDDQQGPAVA
                     SYLAANNKPKLVAVIDDATAYGEGIANEVEKTLKAANIEVLPREKGTDKTTDWKAILT
                     KLRGRNPDAVFYGGMDATGGPLLKQGRELGIKAVFSFGDGACTDKMKELAGEAAEGLL
                     CSQAGIPPQAASKKFLDAYKKKFNADPILYSPFTYDAANLLIEAMKKANSTDPKKYLG
                     ELQKISFTGASGPIAFDDKGDRKDAEITMFSMKGGKLEPIAVIKGGKTMTYDEFAKAA
                     K"
     gene            complement(1551..1862)
                     /locus_tag="VFR57_07615"
     CDS             complement(1551..1862)
                     /locus_tag="VFR57_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0259266.1"
                     /translation="MKLAGTCIALAALAAAAFPAAASEELAKKHACLACHTVDKKMVG
                     PSYKDVAAKYRADKEAPKKLAAKVKNGSQGVWGNVPMPPNTAVPDADVNALVKWILSQ
                     K"
     gene            complement(1859..3841)
                     /locus_tag="VFR57_07620"
     CDS             complement(1859..3841)
                     /locus_tag="VFR57_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0259267.1"
                     /translation="MNRLQGETSPYLRQHAANPVHWYPWGPEALERARAEDKPILLSI
                     GYSACHWCHVMAHESFEDPGVAEVMNRHFVNVKVDREERPDLDQIYQTAHQMLARRTG
                     GWPLTMYLAPDGTPFFGGTYFPKEPRYGLPGFADLCERVAALWREKRAEIAAQNAEVL
                     NALARTLPQRAAQRNELDGEPVRSLLEMLKANFDARFGGFGAAPKFPHPTDLELCLRK
                     GETEIALTTLSHMCEGGIYDQLGGGFYRYSVDAGWRIPHFEKMLYDNGPLLGLLADAW
                     LVNGEQRYAQCAAATAGWLMREMQSPEGGYYSSLDADSEHEEGKFYVWSTDEVQRLLS
                     DEEYAAFAPRYGLDDSPNFENRHWHLHVAKRLESPALGTAKQKLFSAREKRVRPGRDE
                     KVLVSWNALAISGMAHAGRALGNPTWIESARRALRFIRSTMWGNRRLAATYKDGRAHL
                     NAYLDDYALLIAALLEFLQERFSVDELNFAVELAEVLLDQFEDRAEGGFYFTAREHER
                     LIHRPKPGHDHATPSGNAVAAWVLDRLHALTGEARYAAAAERTLELFYPAMREHPGGY
                     AMMAIALDQHLAQPKTLVLRGRPDAVEAWKAQLAREYMPDTLLLAIPDGVPGVPPLLD
                     KPARAGEVNGWLCQGVSCLEPSNDLIHLRQVLKEKT"
     gene            complement(3852..4223)
                     /locus_tag="VFR57_07625"
     CDS             complement(3852..4223)
                     /locus_tag="VFR57_07625"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEU0259268.1"
                     /translation="MIAQFRRFIAVLLAITLVVPLPARAAMLSTESALGPDRERIAQI
                     LDRADAQAQLQAFGVGAAEVKARVAALTDEEAAELAARIGELPSGGVGILGAVLIVFL
                     VLLLTDILGFTKVFPFTRPAK"
     gene            4255..4827
                     /locus_tag="VFR57_07630"
     CDS             4255..4827
                     /locus_tag="VFR57_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEU0259269.1"
                     /translation="MKSLALEVLHFWFGEGAEYGKRHKRWFEKNNVFDAEITERFGSL
                     HEEMLAEKHRDWLEEPRRCLARIIVLDQFPRHIHRGTARAFSSDALALEAAKHFVGKG
                     WDRELLAVERMFAYLPFEHSESLEDQERACTLCEPLLAFPEVADTYRYALAHRDIIRR
                     FGRFPHRNAALGRASTREEIEFLNEPGSSF"
     gene            complement(4824..>5585)
                     /locus_tag="VFR57_07635"
     CDS             complement(4824..>5585)
                     /locus_tag="VFR57_07635"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0259270.1"
                     /translation="DLKPSGRFVAVDFHRAGGVPQVLKILLENGVLHGECMTIHGKTM
                     REMLKDVPSQPRADQEVIRQWSKPLYPEGHLAILKGNLATEGCVAKITGLKSASITGP
                     ARVFDSEPAALKAILARRIKPGDVIVIRYEGPKGGPGMQEMLAPTSALIGQGLGESVG
                     LLTDGRFSGGTWGMVVGHVAPEAYVGGTIALVKERDSITIDAKKRLLQLNVSPKELAA
                     RRKKWKQPRPRYTRGLLAKYMKLVSTASRGAITDG"
BASE COUNT          912 a         1812 c         1775 g         1086 t
ORIGIN      
        1 ccatagccga ccagcatgca gatcggcacc gcgcatccgg tgccgatcag gacgatgacg
       61 aacggcggaa gcccggtcgg cacgagcagg ttgatgatgc tgaacgccac catcgcgccg
      121 atcatcacca cttcaccgtg ggcgaaattg atgagctgga tgatgccgta caccatggtg
      181 tagccaagcg ccaccaccgc gtacaggctt cccgcggtga gcccgttgac cacctgctgc
      241 aggaaggtat ccatgcaaaa aaaacggccc tacgcgggcc gtttttcttt gccagaactt
      301 atggcgccgc gcctacttcg ccgccttcgc aaactcgtcg taggtcatgg tcttgccgcc
      361 cttgatcacc gcgatcggct cgagcttgcc gcccttcatg ctgaacatgg tgatctcggc
      421 gtccttgcgg tcgcccttgt cgtcgaacgc gatgggcccg ctcgcgccgg tgaagctgat
      481 cttctgcagc tcgcccaggt acttcttggg atcggtggag ttcgccttct tcatcgcctc
      541 gatcagcagg ttggccgcgt cgtaggtgaa tggcgagtac aggatcgggt cggcgttgaa
      601 cttcttcttg taggcgtcga ggaacttctt ggacgccgcc tgcggcggga tgccggcctg
      661 cgagcacagc aggccttcgg ccgcctcgcc cgccagctcc ttcatcttgt cggtgcacgc
      721 accgtcgccg aacgagaaca ccgccttgat gccaagctcg cgtccctgct tgagcagcgg
      781 gccgccggtg gcgtccatgc cgccgtagaa caccgcgtcc gggttgcggc cgcgcagctt
      841 ggtgaggatc gccttccagt cggtagtctt gtcagtgccc ttctcgcgcg gcagcacttc
      901 gatgttggcc gccttgagag tcttctcaac ctcgttcgcg atgccttcgc cgtaggcggt
      961 ggcgtcatcg atcaccgcga cgagcttcgg cttgttgttc gcggccagat agctcgccac
     1021 cgcgggccct tgctgatcgt cgcgggcgac tacgcggaac tgcgtcttga acccctgttc
     1081 ggtgagcttg ggattggtcg ccgagcccga tattaccggg atgccggcct ggttgtagat
     1141 cggggaggca ggaatcgaag ttccggagtt caggtgtccg acgacgccga ccaccttggc
     1201 gtcgacgagc ttctgtgcca cggtagtgcc gaccttggga tcggcctggt cgtcctcggc
     1261 gaccaacgta aatttcacgt ccttgccatc aatcttgagc ttggccgcgt tcgcttcctc
     1321 tatggcgagg cgagcgccgt tctcgttgtc cttgccgagg tgcgcgatgc cgccggtgag
     1381 cggcgcgaca tggccgatcc tcacttcagc tccgccgccg ccgctttgct gtccgcagcc
     1441 gaccagcgct accgctgcag caatgacgca aatgccaatt tgagtgttct tcatgagttc
     1501 tcccgggaat tcgtgcgagg ggttatgcgt gcgccccact atcatacctc ttacttctgc
     1561 gagagaatcc acttcaccag cgcgtttacg tcggcgtccg gcaccgcggt attcggcggc
     1621 atcggcacgt tcccccacac cccctggctg ccgttcttga ccttcgccgc gagcttcttt
     1681 ggcgcttcct tgtccgcgcg gtacttggcc gccacatcct tgtaggacgg cccgaccatc
     1741 ttcttgtcca cggtgtggca ggcaagacag gcgtgcttct tcgccagttc ctcgctggcc
     1801 gcggcgggga aggcggctgc cgcaagcgcc gcaagcgcaa tacatgtccc tgcaagcttc
     1861 atgtcttctc cttaaggact tgtctcaagt gtattagatc gttacttggc tccaggcagc
     1921 taacgccctg gcacaaccat ccgttgacct cgcccgcgcg cgctggcttg tcgagaagcg
     1981 gcggcacgcc cgggacaccg tcggggatgg cgagcagcag cgtgtcgggc atgtattcgc
     2041 gcgcgagttg cgccttccac gcctccacgg catccggcct gccgcgcagg acaagggtct
     2101 tcggctgcgc gaggtgctgg tccagggcga tcgccatcat ggcgtatccg ccggggtgct
     2161 cgcgcatggc cggatagaag agctcgagcg tacgctcggc cgcggccgcg tagcgcgcct
     2221 cgcccgtgag cgcatgcagc cggtcaagga cccacgcggc cactgcgttg cccgagggcg
     2281 ttgcgtgatc gtggccaggc ttcggccgat ggatcaatcg ctcgtgctcc cgcgcggtga
     2341 agtagaagcc gccctcggcg cggtcctcga actgatcgag aagcacctcg gccagctcga
     2401 cggcaaagtt gagctcatca accgagaacc gctcctgcag gaactcgaga agcgcggcga
     2461 tcaggagcgc atagtcgtcg agataagcgt tgagatgcgc ccggccgtcc ttgtaggtcg
     2521 cggcgagccg gcggttaccc cacatcgtcg agcggatgaa cctgagcgcg cgacgtgcag
     2581 actcaatcca ggtcgggttg ccgagcgcgc gcccggcatg cgccatgccg ctgatggcga
     2641 gcgcgttcca ggaaacgagt actttctcgt cgcggccggg gcgcacgcgc ttctcgcgcg
     2701 cagaaaagag cttttgcttg gcggtcccca gagctggact ttcaagcctt ttcgccacgt
     2761 gaagatgcca atgccggttc tcgaagttgg gggaatcgtc gaggccgtag cgcggcgcga
     2821 acgccgcgta ttcctcgtcg ctgaggagcc gctgcacttc gtcggtgctc cagacgtaga
     2881 acttgccttc ttcgtgctcg ctgtcggcgt cgagcgagga gtagtagccg ccttcgggcg
     2941 actgcatttc ccgcatcaac cagccggcgg tggcggcggc gcactgcgcg tagcgctgct
     3001 cgccgttcac aagccaggca tcggcaagca ggccgagcaa ggggccgttg tcgtagagca
     3061 tcttctcgaa gtgcgggatc ctccacccgg catcgacgct gtaacggtag aacccgccgc
     3121 cgagctgatc gtatatgccg ccttcgcaca tgtggctgag cgtcgtcagt gcgatttcgg
     3181 tctcaccctt gcgcaggcaa agctccaagt cggtggggtg cggaaatttc ggcgccgcgc
     3241 cgaagccgcc gaagcgcgca tcgaaatttg ccttcagcat ttcgagcaag ctcctcactg
     3301 gctcgccgtc gagctcgtta cgctgagcgg cgcgctgcgg aagggtgcgc gcgagcgcgt
     3361 tcagcacctc ggcgttctgc gccgcgattt cggcgcgctt ctcgcgccag agtgccgcga
     3421 cgcgctcaca gagatcggcg aacccgggca ggccgtagcg tggctccttc gggaagtacg
     3481 tgccgccgaa gaacggcgtt ccgtcgggcg ccaagtacat ggtgagcggc cagccgccgg
     3541 tgcgccgggc gagcatctgg tgcgcagtct ggtagatctg gtcgaggtcg ggccgctcct
     3601 cgcggtccac cttcacgttg acgaagtgcc ggttcatcac ctcggcgacg ccggggtcct
     3661 cgaaactctc gtgcgccatg acgtggcacc agtggcatgc cgagtaaccg atcgagagca
     3721 ggatcggctt gtcctcggcg cgtgcgcgct ccagcgcctc gggtccccag ggataccagt
     3781 ggactgggtt tgctgcgtgc tgccgcaggt agggcgaagt ctcgccctga agtctgttca
     3841 ctccacgcgg ttcacttcgc cgggcgcgta aacgggaaga ccttggtgaa gcccaggata
     3901 tcggtgagga gcagcaccag gaatacgatg aggacggcac cgaggatgcc caccccaccc
     3961 gaaggcaact cgccgatgcg tgcggcaagc tccgccgctt cttcgtcggt gagcgcggcg
     4021 acgcgggcct tgacctcggc cgcgcctacg ccaaaggctt ggagctgcgc ctgcgcatcg
     4081 gctcgatcca ggatctgcgc gatacgttcc cggtccgggc cgagagcgct ctcggtgctc
     4141 agcatcgcgg cgcgcgccgg aagcggcacg acgagcgtaa tggcgagcaa gactgcaatg
     4201 aagcggcgga actgagcgat catgacgtct cccttcttgg ttggctcttg gtcgatgaaa
     4261 agcttagcac ttgaggtatt gcatttctgg ttcggggaag gcgccgagta cggaaagcgg
     4321 cataagcgct ggttcgaaaa gaacaacgtc ttcgacgccg agattacgga gcgcttcggt
     4381 tccttgcacg aggagatgct tgcggaaaag caccgggact ggctggaaga gccacgccgc
     4441 tgcctcgcgc gcatcatcgt gctcgatcag tttccgcgcc acatccatcg cgggactgcc
     4501 cgcgcctttt ccagcgacgc gctcgccctc gaggccgcga agcacttcgt cggcaagggc
     4561 tgggaccgcg aattgttggc ggtagagcgc atgttcgcct acctgccgtt cgagcacagc
     4621 gaatcactcg aggaccagga acgggcgtgc acgctctgtg aaccgctgct tgctttccca
     4681 gaggtcgccg acacctatcg ctacgccctc gcgcaccgcg acatcatccg ccggttcggg
     4741 cgcttcccgc atcgcaatgc ggctctcggg cgcgccagca cccgcgaaga gatcgagttc
     4801 ctgaacgaac ctgggtcgag cttctagccg tctgttatcg cgccccgcga agcggtggac
     4861 acgagcttca tgtacttcgc cagtaatccc cgcgtgtagc gcggcctggg ctgtttccat
     4921 ttcttgcggc gcgcggcgag ctccttgggc gaaacgttaa gctgcagcaa gcgcttcttg
     4981 gcgtcgatgg tgatggagtc gcgttccttg acgagggcga tcgtgccgcc gacgtaggcc
     5041 tcgggtgcga catgaccgac gaccattccc caagtgccgc cggagaatcg cccatccgtc
     5101 agaaggccga ccgattcgcc caatccctgc cctataagcg ccgaagtggg cgctaacatt
     5161 tcctgcattc ccggtccgcc tttgggccct tcatagcgaa tcacgattac atcgcctggc
     5221 ttgatccgcc tggcgaggat ggctttcagc gccgccggct ccgaatcgaa cacacgcgcc
     5281 gggccggtga tcgacgcgct cttcaggccg gtgatcttcg ccacgcaacc ttccgtcgcg
     5341 aggttgccct tgaggattgc gagatggccc tcggggtaga gcggtttgga ccactggcgg
     5401 atcacttcct ggtcggcgcg cggctgcgaa ggcacgtcct tcagcatttc gcgcatagtc
     5461 ttgccgtgga tcgtcatgca ctcgccgtgc aggacgccgt tctcgagcag gatcttcagc
     5521 acctgcggta cgccgccggc gcggtggaaa tccaccgcca cgaagcgccc ggagggcttc
     5581 aagtc
//
LOCUS       DASTPD010000230         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_532400, whole genome shotgun sequence.
ACCESSION   DASTPD010000230 DASTPD010000000
VERSION     DASTPD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532400"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFR57_07640"
     CDS             <1..273
                     /locus_tag="VFR57_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0259271.1"
                     /translation="NVVLNTAFGQPTVAVDRHIFRVANRTGLAPGKDSLEVEEKLIKV
                     TPPEYLKNAHHWLILHGRYVCLARKPGCPQCLIRDLCEFRPKTKEK"
     gene            279..704
                     /locus_tag="VFR57_07645"
     CDS             279..704
                     /locus_tag="VFR57_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEU0259272.1"
                     /translation="MFNPTREQVREMFFSAWRRYREGLPLAGMEGLALEVILLHPEYH
                     EALEDPDRTRDKDYIDESNPFLHMSLHVALEEQLSIDQPPGIVARWRQLLERYGDRHD
                     ALHQALECLAETVWRAQRDSAPPDAQSYLECLANRALRR"
     gene            803..1405
                     /locus_tag="VFR57_07650"
     CDS             803..1405
                     /locus_tag="VFR57_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0259273.1"
                     /translation="MNSRSSLLAFVMAACAAIMACASPARAEDVSDSIILVAKRHLQD
                     KLYGSTILIARPIGGERHVGFIVNKPTQMTLGKLFPQHEPSRKVADPVFLGGPVSPEV
                     IFALVQSRESPGGRSIRILDDLYLAIDSAIVDRVIEKQPSQARFLAGMVLWRPGELNE
                     ELRRGFWCMQSARAEIVLRKPTDTMWEDLVGRCERQANAV"
     gene            complement(1410..1736)
                     /locus_tag="VFR57_07655"
     CDS             complement(1410..1736)
                     /locus_tag="VFR57_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0259274.1"
                     /translation="MNALLSIVLVFGVSTSLYAKGNAEAGKAKAAQVCAACHGPDGNK
                     PSDPTQPILAGQYYDYLVRALTDYKIGRRNNPIMKGFAGQLSKQDIEDLAAWFSSQKS
                     NLHDQR"
     gene            complement(1733..2083)
                     /locus_tag="VFR57_07660"
     CDS             complement(1733..2083)
                     /locus_tag="VFR57_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0259275.1"
                     /translation="MLGSILRVMPATILGAALACGALAQDKQAPAVAKVGQGRDNALA
                     MCVGCHGIPGYKTAFPEVYHVPKIAGQQPGYIVNALKAYKSGERSHPSMRGIAAGLSE
                     EDMKQLADYYGGGK"
     gene            2154..2996
                     /locus_tag="VFR57_07665"
     CDS             2154..2996
                     /locus_tag="VFR57_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0259276.1"
                     /translation="MRFKGSASYVATEDLTLAVNAAITLQRPLLVKGEPGTGKTMLAV
                     EVARALGVPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVHDIHNYIVRGMLWQ
                     AFTSDAPVVLLIDEVDKADIEFPNDLLRELDRMEFYVYETRELVKAKHRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKQTMEKIVSVHFPNLKKSLLREAMEAFFELREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSKDRKTIIPPLHGALLKNEQDVHLFERLVFMT
                     RAKA"
     gene            2983..3633
                     /locus_tag="VFR57_07670"
     CDS             2983..3633
                     /locus_tag="VFR57_07670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0259277.1"
                     /translation="MPRPEVTLTFDNGPEPGVTEQVLAVLKQANVSATFFVLGSKLAD
                     PVRRRLARQAHAEGHWIGNHTMTHRTPLGSVAEPGVARTEILDTEALIGALAHPDRLF
                     RPFGGGGELGPHLLNQEALEVLTAGKMTCVLWNAVPRDWADPDGWAEVALEQCLAHRH
                     TLLVLHDLPTGAMRRLGAFIASVLQHGGVFRQDFPPECVPIRRGEAVASLEPYVSA"
     gene            3661..4512
                     /locus_tag="VFR57_07675"
     CDS             3661..4512
                     /locus_tag="VFR57_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259278.1"
                     /translation="MGYHNNPTEPRLRLPGLSCDAHFHVLGPKDKFPFDAKAPYTPFD
                     APKDVLIDRHMFLGIERGVVVQSAAHGFDHSAAADFLAEYQGSYVGVALARVNTDMAE
                     LKKLHGQGFRGVRFNFVQHLGGGPALSDVMKLASKLAVLGWHLQLYVDPSMILKMTPE
                     LKLSPVPVVIDHMGHIDASHGLDQPGFTHLLKLMEDKRFWVKVSGLERASRQGSPYAD
                     AVPFARKLVTEFGDRVVWGSDWPHPNLSSVPDDGVLVDLIAEFANEAQRQALLVDNPQ
                     RLYKFRA"
     gene            4509..>5350
                     /locus_tag="VFR57_07680"
     CDS             4509..>5350
                     /locus_tag="VFR57_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259279.1"
                     /translation="MRRLVFFLGALFALAAWAQPYPSKPVRLIVGFPAGGPADIFGRT
                     LAQGMAPELGQPVVVENVSGVGGVLGVDRAAKAPPDGYTLCINSASPLVIAPYSLSKI
                     PYDVKKDFAFITLVVRVPEVVAVHPSLPVNSLSELIGYARANPGKVNFGSAGSGSITH
                     LAGELLKAEAKVELVHVPYKGAAPAVNDLLGGQVQMGIFDVPVLLGHIRSGKLKAFAL
                     TSRQRAASLPEVPTTAELGYPNVNSDNWYGLVAAAGTPAEVQRRIHAAAVAALKSPAV
                     EEQFA"
BASE COUNT          933 a         1790 c         1705 g          922 t
ORIGIN      
        1 aacgtggtgc tgaacacggc gttcggccag ccgaccgtgg cggtcgacag gcacattttc
       61 cgggtcgcga atcgcaccgg cctcgcaccg gggaaagatt ccctggaagt cgaggagaag
      121 ctgatcaagg tcacgccgcc ggagtacctg aagaacgcgc accactggct gatcctgcac
      181 gggcgctacg tctgccttgc gcgcaagccc ggctgcccgc agtgcctgat ccgcgacctc
      241 tgcgagttca gaccgaaaac caaggagaag taaggcggat gtttaaccct acccgcgagc
      301 aggtccggga gatgttcttc tcggcgtggc gcaggtaccg ggaaggcctc ccgcttgcgg
      361 gcatggaagg gcttgcgctc gaggtgatcc tgcttcaccc ggagtaccac gaggcgctcg
      421 aagatcccga ccgcacacgg gacaaggact acatcgacga gtcgaaccct ttcctgcaca
      481 tgagcctgca cgtcgcgctg gaggagcaac tttcgatcga ccagccgccg ggcatcgtgg
      541 cgcgatggag gcagctcctc gagcgctacg gcgaccggca tgacgcgctg caccaggcgc
      601 tcgagtgcct ggccgagacc gtgtggcgcg cgcagcgcga cagcgcgccc cccgacgcgc
      661 agtcttacct ggaatgcctt gccaaccggg cgttacgccg gtagttctcc cgctacgccc
      721 gcaagaacta cgcgtcgctc ggtgcggcaa gcatattgct tggcgcatga tgacccgaac
      781 aacactggag agaccgcacg ccatgaactc acgctcttcc ctattggcat ttgtcatggc
      841 agcatgcgcg gccatcatgg cgtgcgcgtc gccggcgcgc gcggaggacg tatcggattc
      901 gatcatcctc gtcgccaagc ggcacctgca ggacaagctc tacggctcga ccatcctcat
      961 cgcgcgcccg atcggcggag agcgccacgt cggcttcatc gtcaacaagc ccacccagat
     1021 gacgctcggc aagcttttcc cgcagcatga gccgtcgcgc aaagtggcgg acccggtgtt
     1081 cctcggcggc ccggtgagcc cggaggtgat ctttgcgctg gtgcagagcc gcgagagccc
     1141 cggcggccgc tcgatccgga tcctggacga cctctatctc gccatcgaca gcgccatcgt
     1201 cgatcgcgtg atcgaaaagc agccctcaca ggcgcgcttc ctcgccggca tggtgctgtg
     1261 gcgtccgggg gagctgaacg aggaactgcg gcgcggcttt tggtgcatgc agagcgccag
     1321 ggccgagatc gtccttcgca agccgaccga caccatgtgg gaagaccttg tcgggcgctg
     1381 cgagcgccaa gcgaacgcgg tctaggagtt caacgctggt cgtgcaggtt ggacttctgg
     1441 ctcgagaacc acgccgccag gtcctcgatg tcctgctttg aaagctggcc ggcgaagccc
     1501 ttcatgatgg gattgttgcg ccggccgatc ttgtagtcgg tcagcgcacg caccagatag
     1561 tcgtaatact gcccggccag gatcggctgg gtggggtccg agggcttgtt gccgtccggg
     1621 ccgtggcacg cggcgcacac ttgcgcggct tttgccttgc cggcctcggc atttcctttg
     1681 gcatagaggg aagtggaaac gccgaaaacc aacacgatgg aaagaagcgc gttcatttgc
     1741 cgcccccgta gtaatcggca agctgcttca tgtcttcttc gctcagcccg gccgcgatgc
     1801 cgcgcatcga cgggtgcgag cgctctccgg acttgtaggc cttgagcgcg ttcacgatgt
     1861 agcccggttg ctggcctgcg atcttcggca cgtggtagac ctccgggaac gcggtcttgt
     1921 agccgggaat gccgtggcag ccgacgcaca tcgcgagcgc gttgtctcgc ccctgcccga
     1981 ccttcgccac ggcaggcgcc tgcttgtcct gcgcaagcgc gccgcaggcg agcgcggcgc
     2041 caagaatcgt cgcaggcata accctgagaa tggacccgag catgcttctc ttcttggaga
     2101 ttgttgagag cccaaattgt agccaaaaaa ctcgctaggc tagaatcggc cgcatgcgtt
     2161 tcaagggatc ggcgtcctac gtcgccaccg aggacctgac gctcgccgtc aacgcggcga
     2221 tcacgctgca gcggccgctg ctcgtcaagg gagagcccgg caccggaaag acgatgctcg
     2281 cggtggaggt ggcgcgcgcg cttggcgtgc cgctgctcga atggcacatc aagtcgacca
     2341 ccaaggcgca gcaaggactg tacgagtacg acgcggtaag ccggctgcgc gactcgcaac
     2401 tcggcgacgc gcgcgtgcac gacatccaca actacatcgt cagggggatg ctgtggcagg
     2461 cgttcacctc cgatgcgccg gtggtgctcc tcatcgacga ggtcgacaag gccgacatcg
     2521 agttcccgaa cgacctcctg cgcgagctcg accgcatgga gttttacgtc tacgagacgc
     2581 gggagctcgt gaaggcgaag caccggccga tcgtgttcat cacctcgaac aacgagaagg
     2641 agctccccga cgcgttcctg cgccgctgct tcttccatta catccgcttc cccgataagc
     2701 agacgatgga gaagatcgtc tcggtgcact tccccaacct caagaagtcg ctgctgcgcg
     2761 aggcgatgga agcctttttc gaactgcgcg aggtgcccgg gctgaagaag aagccttcga
     2821 cctcggagct tctcgactgg ctgaagctgc tgctcgccga agacatcccg cccgaggcgc
     2881 tgcgctcaaa ggaccgcaag acgatcattc cgccgctgca cggcgcgctt ctcaagaacg
     2941 agcaggacgt gcaccttttc gagcggctcg tgttcatgac tcgtgccaag gcctgaggtc
     3001 acgctcactt tcgacaacgg acccgagccc ggcgtcactg agcaggtctt ggcagtcctg
     3061 aagcaagcga acgtcagcgc cacctttttc gtcctcggct cgaagctcgc cgatcccgtg
     3121 cgccggcgcc tcgcgcggca ggcgcacgcc gaaggccact ggatcggcaa ccacaccatg
     3181 acgcaccgaa cgccgctcgg ctccgtagcc gagccgggcg tcgcgcgcac ggagattctc
     3241 gataccgagg cgctgatcgg cgccctcgcg catccggacc gcctgtttcg tcctttcggc
     3301 ggaggcggcg agctcggccc gcacttgctg aaccaggaag ccctcgaagt gctgaccgca
     3361 ggaaagatga cctgtgtgct ctggaacgcg gtaccgcggg actgggccga ccccgacggc
     3421 tgggccgagg tggcgctcga gcagtgcctc gcccaccggc atacgctgct cgtgctccac
     3481 gacctgccga cgggcgcgat gcgacgcctc ggcgccttca tcgccagcgt gctccagcac
     3541 ggcggcgtct ttcgccagga ctttccgcca gagtgcgtcc cgatccggcg cggggaggcc
     3601 gtcgcctcgc ttgagcctta cgtcagcgct tgatctttag acatacaatc gcgcggcaac
     3661 atgggctatc acaacaaccc caccgaaccc aggctgaggc ttcccgggct ctcgtgtgat
     3721 gcccattttc acgtcctcgg gccgaaggac aaattcccgt tcgacgccaa ggcgccgtac
     3781 acgcccttcg atgcaccgaa agacgtgctc atcgaccgtc acatgtttct cggcatcgag
     3841 cgcggcgtgg tggtgcagtc ggcagcgcac ggcttcgacc actcggcggc ggccgacttc
     3901 ctcgccgagt atcagggcag ctatgtcggc gtcgcgctcg cgcgcgtgaa tacggacatg
     3961 gcagagctca agaaactgca cggtcagggg ttccgtggcg tgcgcttcaa tttcgtgcaa
     4021 caccttggcg gcgggccggc gctctccgac gtgatgaagc tcgcaagcaa gctcgcggtg
     4081 cttggctggc accttcagct ctacgtcgac ccgtcgatga tcctcaagat gacgcctgag
     4141 ctcaagcttt cgccagtgcc ggtggtgatc gaccacatgg ggcacatcga cgcctcgcac
     4201 gggctcgacc agccgggatt cacgcatctt ctcaagctga tggaggacaa gcgcttctgg
     4261 gtcaaggtga gcggcctgga gcgcgcctct cgtcagggct cgccctacgc cgacgccgta
     4321 cccttcgcgc gcaagctggt caccgagttc ggcgaccgcg tggtctgggg cagcgactgg
     4381 ccgcacccca atctgagctc ggtgccggac gacggggtcc ttgtcgatct catcgccgag
     4441 ttcgcgaacg aggcgcagcg ccaggcgttg ctcgtggaca acccgcagcg gctgtacaag
     4501 ttccgcgcat gagacgcctt gttttcttcc ttggcgcgct tttcgcgctg gccgcgtggg
     4561 cgcagcccta cccgagcaag ccggtgcggc tcatcgttgg cttcccggcc ggcggaccgg
     4621 ccgacatctt cggccgcacg ctcgcgcaag gcatggcgcc cgagcttggc cagccggtcg
     4681 tggtggagaa cgtctccggg gtcggcgggg tgctgggcgt cgaccgcgcc gcgaaagccc
     4741 cgcccgacgg ttacacgctt tgcatcaaca gcgcctcgcc gctcgtgatc gcgccctact
     4801 cgctctccaa gataccgtac gacgtgaaga aagacttcgc gttcatcacg ctggtggtgc
     4861 gcgtgcccga ggttgttgcg gtacacccgt cgctgccggt caactcgcta tccgagctca
     4921 ttggctacgc cagggcaaac cccggcaagg tgaacttcgg ctcggccggc agcgggagca
     4981 tcacccacct cgccggcgag ctgctcaagg ccgaagcgaa ggtcgagctg gtccacgtcc
     5041 cctataaggg cgcggccccg gcggtaaacg acctcctcgg cggccaggtg cagatgggga
     5101 ttttcgacgt gccggtcctg ctcggccaca ttcgcagcgg caagctgaag gcgttcgcac
     5161 tgacgagcag gcagcgcgct gcctcgcttc ccgaggtgcc gaccacggcc gagctcggct
     5221 atcccaacgt caactccgac aactggtatg gcctggtcgc ggcggccggc acccccgccg
     5281 aggttcagcg gcgcattcac gccgccgcgg tcgcggcgct caagtcaccg gcggtggagg
     5341 agcaattcgc
//
LOCUS       DASTPD010000231         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1349283, whole genome shotgun sequence.
ACCESSION   DASTPD010000231 DASTPD010000000
VERSION     DASTPD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349283"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFR57_07685"
     CDS             complement(<1..229)
                     /locus_tag="VFR57_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0259280.1"
                     /translation="MSVYTPVSEAELREWLRNYSVGRLASCEPIKAGIENTNYFVTTT
                     QGRYVLTLFERLPAEELPFYLELMAHLARHGI"
     gene            complement(243..575)
                     /locus_tag="VFR57_07690"
     CDS             complement(243..575)
                     /locus_tag="VFR57_07690"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEU0259281.1"
                     /translation="MRLALLVGLLFILSASPAHAQGRPPKLEPLPEPPPPPPMSVDEP
                     AVRIPVQEGDKVEEVREGGRVVMLKVTPPHGKPYYLVDTTGNGNWMRRDSLDDGVRVP
                     MWPIHTWD"
     gene            complement(591..1307)
                     /locus_tag="VFR57_07695"
     CDS             complement(591..1307)
                     /locus_tag="VFR57_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU0259282.1"
                     /translation="MNQDRQPKLAELAKEVPAREAWRVFGIMSELVEATERLVGIHPA
                     VSVFGSARVKPGTPYYQLAEETARRLSDAGFAVVTGGGPGLMEAASRGAFGGGGLAVG
                     LNIDLAGEQPASRFQHISLRFKHFFTRKAMFAKVATAFIVLPGGYGTLDELFEALTLV
                     QTGKTRQMPIILVHEPYWRGLLAWMRERLAAEGMIDPADIDLVQVYDEPQQVVDAIFA
                     FYEKRGFAPSPAEREVLLNL"
     gene            1336..>3264
                     /locus_tag="VFR57_07700"
     CDS             1336..>3264
                     /locus_tag="VFR57_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU0259283.1"
                     /translation="MLVDGSSYLYRAFHALPGLKSPKTGEPTGAIYGVLNMLRKLAAD
                     YKPAALACVFDAKGRTFRDADYADYKANRSPMPDELGVQVAPLHEAVRALGWPVLIIE
                     GVEADDVIATLAETAKRDGWRTVISTGDKDFAQLVDECVTLVNTMSNELLDVAGVKSK
                     FGVPPEKFLDYLTLIGDQIDNIPGVDKVGPKTACKWIEQYGSLEGVLAHADEISGLAG
                     ENLRKMRDWLPKARQLLTIKRDVPLSVSIHDLHLNGNPAKQRELYERFGFKTWLREVE
                     GSASPSPEPIAPATVEPRKYRALLTEDEFKKLVLKIEAADIVGFDTLCDGDEPMAARL
                     TGMSFAFGDEAVYLPLTHDYPGAPKQLDLSTAFALLKPWLERADCRKVGEDVKFDTHV
                     LANHGVRLAGCVHDTLLESYVLEVHEKHDLAMLAQRHCGRAVPSREEVTGKGVGRIAF
                     ASVDIVRATEYAAQRTDCSLSLHQLLFGKIQGDDKLSFVYNKIEMPVLPVLWRMERNG
                     VLLDRARLEAQSHELGKEILQKEREAYAAAGQPFNLGSPKQIQEILFDRQKLPVKKKT
                     PSGQPSTDEDSLAELALDHPLPRLILEHRALSKLKSTYTDKLPKSINERTGRVHTTYS
                     QITAVTGRLSSNEPNLQNI"
BASE COUNT          617 a         1079 c         1030 g          538 t
ORIGIN      
        1 gaatgccgtg gcgcgcgaga tgggccatca gctcgaggta gaagggcagc tcctcggccg
       61 gcagccgctc gaacagcgtc aggacgtagc ggccctgggt ggtggtgacg aagtagttgg
      121 tgttttcaat gcccgccttg ataggctcgc atgatgcgag ccggccgacg gagtagttcc
      181 tgagccattc gcgcagctcg gcctcggaga cgggggtgta gacggacaag gcggggagag
      241 gctcagtccc acgtgtggat cggccacatc ggcacgcgca ccccgtcgtc gagcgaatcg
      301 cggcgcatcc agttgccgtt gcccgtggtg tcgacaaggt agtagggctt gccgtgcggc
      361 ggcgttacct tgagcatcac cacgcggccg ccttcgcgca cttcctcgac cttgtcgcct
      421 tcctgcaccg ggatgcgcac tgcgggctcg tcgaccgaca tcggcggagg cggcggaggc
      481 tcgggcagcg gctcgagctt gggcggacgc ccttgcgcat gtgccggcga cgcggacagg
      541 atgaagagaa gccctaccag cagcgcaagc cgcattggat cgcgattcta ctagaggttc
      601 agcagcacct cgcgctcagc aggcgacggc gcgaagcctc gcttctcgta gaacgcgaag
      661 atcgcgtcca ccacctgctg cggctcgtca tagacctgca cgaggtcgat gtctgcggga
      721 tcgatcatcc cttcggcggc gagccgctcg cgcatccagg cgagaaggcc gcgccaatac
      781 ggctcgtgca ccaggatgat cggcatctgg cgcgtcttgc cagtctgcac cagggtgagg
      841 gcctcgaaca gctcatcgag cgtgccgtag ccgcccggaa gaacgatgaa cgcggtcgcg
      901 accttggcga acatggcctt gcgcgtgaag aaatgcttga agcgcaggct gatgtgctgg
      961 aagcggctcg cgggctgctc gcccgccagg tcgatgttca ggcccaccgc gagtccgccg
     1021 ccgccgaacg cgccccggct cgccgcctcc atgaggcccg ggccgccgcc ggtgacgacg
     1081 gcgaagccgg cgtcggaaag ccggcgcgcc gtttcttccg cgagctggta gtacggcgtt
     1141 cccggcttga cgcgcgcgct gccgaacacc gagaccgcgg gatggattcc caccagccgc
     1201 tcggtcgcct cgaccaactc tgacataatg ccgaaaaccc gccacgcctc gcgcgcggga
     1261 acctctttcg ccagctccgc cagcttcggt tgcctgtcct gattcatgtc gggaaaagat
     1321 cctgagaaaa cgttattgct ggtcgacggc tcgtcatacc tgtaccgcgc ttttcatgcg
     1381 cttccggggc tgaagagccc aaagaccggc gagccgaccg gcgcgatcta cggggtgctg
     1441 aacatgctgc gcaagctcgc agccgattat aagccggccg ctctcgcctg cgtgttcgac
     1501 gccaagggca ggaccttccg cgatgcggac tatgccgatt acaaggcgaa ccgctcgccg
     1561 atgcccgacg agctcggtgt gcaggtcgcg ccgctgcacg aggcggtgcg cgcgctcggc
     1621 tggccggtgc tcatcatcga aggagtagag gcggacgacg tcatcgccac gctcgccgag
     1681 actgccaagc gcgacggctg gcgcacggtg atctcgacag gcgataagga cttcgcccag
     1741 ctcgtcgacg agtgcgtgac gctcgtcaat accatgtcga acgagctgct cgacgtcgcg
     1801 ggagttaaga gcaaattcgg cgtgccgccc gagaagttcc tcgactattt gacgctcatc
     1861 ggcgatcaaa tcgacaacat ccctggggtc gacaaggtcg gccccaagac cgcgtgcaag
     1921 tggatcgagc aatacggctc gctcgaaggc gtgcttgcgc acgccgatga gatcagcggg
     1981 ctcgccggcg agaacctgcg caagatgcgc gactggctgc cgaaggcgcg tcagctcctc
     2041 accatcaaac gcgacgtgcc gctttccgta tcgattcacg accttcacct caacggcaac
     2101 cccgcgaaac agcgcgagct ctacgagcgc tttggcttca agacctggct gcgcgaggtg
     2161 gagggctctg cgtcgccgtc gcccgagccc atcgctccgg caaccgtcga gccacgtaag
     2221 taccgcgctt tgctcacgga ggatgaattc aagaagctcg tgctgaagat cgaggccgcc
     2281 gatatcgtcg gattcgacac gctttgtgac ggcgacgagc cgatggctgc gcgcctcacc
     2341 ggcatgtcgt tcgcattcgg cgatgaagcc gtctacctgc ctcttactca tgactatcca
     2401 ggtgccccga aacagctaga tctttccacc gcatttgcgc tgctgaagcc gtggctcgag
     2461 cgggcggact gcagaaaggt cggcgaggac gtgaagttcg acacccacgt cctcgcaaac
     2521 cacggcgtgc gcctcgcggg ctgcgtgcac gacacgctgc tcgagtccta cgttctcgaa
     2581 gtccacgaga agcacgacct cgccatgctt gcgcagcgcc attgcggccg tgccgtgcca
     2641 agcagggagg aggtgaccgg aaagggtgtg gggcgcattg cgttcgcttc cgtcgacatc
     2701 gtgcgcgcca ccgagtacgc cgcgcagcgc accgactgct cgctctcgct tcaccagctg
     2761 ctcttcggga agattcaggg cgacgacaag cttagcttcg tctacaacaa gatcgagatg
     2821 ccggtgctgc cggtactctg gcgcatggag cgcaacggcg tgctactcga ccgcgccagg
     2881 ctcgaagccc agagccacga gctgggcaag gaaattcttc agaaagaacg ggaagcctat
     2941 gcagctgcgg gccaaccctt caacctcggc tcgcccaagc agatccaaga gatcctgttc
     3001 gaccggcaga agctcccagt aaagaagaag acgccctcgg gccagccgtc aaccgacgag
     3061 gactcgctcg ccgagctcgc gctagaccac ccgctgccgc gtcttatcct cgagcaccgc
     3121 gcgctctcca agctcaagtc cacgtacacc gacaagctgc cgaagagcat caacgaacgc
     3181 accggccgtg tgcacaccac gtactcgcag atcaccgcgg tcaccggcag gctctcgtcc
     3241 aacgagccca acctgcagaa catc
//
LOCUS       DASTPD010000232         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_941090, whole genome shotgun sequence.
ACCESSION   DASTPD010000232 DASTPD010000000
VERSION     DASTPD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941090"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..588
                     /locus_tag="VFR57_07705"
     CDS             124..588
                     /locus_tag="VFR57_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0259284.1"
                     /translation="MTSHSKWKFDGVRVVHSNELDINTPQTPGMNRAAAITHARAGAE
                     KLWAGTVVIHPKAKTGAHHHGPVESVIYVVSGKARMRWGEKLEFVAEAGPGDFIYVPP
                     YVPHQEINASDTEPLSCVLCRSGQEPVVVNLPDLPVAEKPEEVRWVDDIHKK"
     gene            complement(635..1468)
                     /locus_tag="VFR57_07710"
     CDS             complement(635..1468)
                     /locus_tag="VFR57_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0259285.1"
                     /translation="MIIEPLAGALGAEIRGIDLKEPAERNYHAIHEAFLKYSVLVFRD
                     QELQPADLMRVGTRFGEPCYYPFVTGIDGYPFIFEVVKEENETVNFGGNWHSDTTYLP
                     QPPRATLLYALETPTHGGDTLFANTTAAYDALSEAMRTFVDKLVGVNSASLKVTGGRR
                     KLHSPIGGMKVHDTEKADELEAEHPVARTHPGTGRKALYVSRSHTIRFRDMSEEESRP
                     LIDFLQAHQIRPEFTCRVRWSPGTLTVWDNRCTQHCAVNDYHGQRRRMRRLTVGAETP
                     R"
     gene            1480..2535
                     /locus_tag="VFR57_07715"
     CDS             1480..2535
                     /locus_tag="VFR57_07715"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0259286.1"
                     /translation="MTKRIAIVGAGALGGYVGGTLAHLGFDITLIDPWPEHVEIIRSR
                     GLELDGVTPEEKFAVREAKTLHVTEVQSLAKTPIDVAMVAVKSYDTHWATALIAPYLS
                     ERGYVVSLQNCMNEETIAGVVGWGRTVGAVASFISVDLYEAGRIRRTVPKGGDKHTVF
                     RIGEPHGAITPRVEELVEWFRGIDSSKVTTNLWGERWSKLVQNAMGNGVTAATGLTSG
                     ACLHHPAIRRFQIELAAEAVRVGQALGYQLEKIRGAAPELYVRAADGDSQALSELEAL
                     IVPKAGSNPRADIQRPSMAQDILKGRRTEIDAMNGYIARKGAEVGVPAPSHKKLAEIV
                     TRIERGEVKPSPSLLSI"
     gene            complement(2504..>3106)
                     /locus_tag="VFR57_07720"
     CDS             complement(2504..>3106)
                     /locus_tag="VFR57_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0259287.1"
                     /translation="GNVLTMLLAGEDTTANTLAWMIYLLYRHPEALRRAREEVLGKDV
                     HHYESMTALPFVDACINETMRLKPVAPILLLQPTRDTVIAGIEVPKGTILMCLMRAGA
                     IDERRFPDARAFDPARWLSGATAASPKRVSMPFGAGPRLCPGRYLAILEMKMVMTMLL
                     NNFEIESVGTPDGGEAREHLAFTMYPEDLRLKLKATKARA"
BASE COUNT          544 a          971 c         1039 g          552 t
ORIGIN      
        1 ggacctgcgg gacatggcgg cgcgcgagcc ctgtcagttc gagcgccacg gctacttcgt
       61 cgcggactcc gatgggacga gcttcaaccg caccgtgacg ctcagggact cctggagcaa
      121 atcatgactt cccattccaa atggaaattc gacggagtgc gggtcgtgca ctccaacgag
      181 ctcgacatca acacgccgca aacgccgggc atgaaccgcg ccgcggccat tacacatgcg
      241 cgcgccggag cggagaagct ctgggccgga acggtggtga ttcatccgaa ggcgaaaact
      301 ggggcccacc atcatggacc cgtggagagc gtgatctatg tcgtgagcgg caaggcgcgc
      361 atgcgctggg gcgagaagct cgagttcgtg gccgaggccg gccccggcga cttcatctac
      421 gtgccgccct acgtgccgca ccaggaaatc aacgctagcg ataccgagcc gctctcgtgc
      481 gtgctttgcc gcagcgggca ggagccggtc gtcgtcaacc tgccagactt accggtggcc
      541 gagaaaccgg aagaagtccg ctgggtcgac gatattcaca agaaatagcc tggggtcaga
      601 ccccgcttca ctcggttggg tccgaccccg attttcacct tggggtttcc gctccgacgg
      661 tcaggcgccg catgcggcgg cgctggccgt ggtagtcgtt aaccgcacag tgctgcgtgc
      721 agcggttgtc ccatacggtc agcgttccgg gcgaccagcg cacgcggcag gtgaactcgg
      781 gccggatctg gtgcgcctgc aggaagtcga tcagcggtcg gctctcctct tcgctcatgt
      841 cgcggaaacg gatggtgtgg ctcctcgaca cgtaaagcgc cttgcgcccc gtcccggggt
      901 gcgtgcgcgc caccgggtgc tcggcctcga gttcgtcggc cttctcggtg tcgtgcacct
      961 tcatgccgcc gatcggcgag tgcagcttgc ggcggccgcc ggtcaccttg aggctcgcgc
     1021 tgttcacgcc gaccaacttg tctacgaaag tgcgcatcgc ctccgacagc gcgtcgtagg
     1081 ccgcggtggt attcgcgaac agtgtatcgc cgccgtgcgt cggcgtttcg agcgcataga
     1141 gcaacgtcgc gcgcggcggc tgcggcaggt atgtcgtgtc cgaatgccag ttgccaccga
     1201 agttgaccgt ctcgttttct tccttcacga cctcgaagat gaaagggtag ccgtcgatgc
     1261 cggtgacgaa cgggtagtag cacggctcgc cgaagcgggt gccgacgcgc atgaggtcgg
     1321 cgggctgtag ttcttggtcg cggaaaacca gtaccgagta ctttagaaac gcttcgtgaa
     1381 tcgcgtggta gttgcgctcg gcgggttctt tcaaatcgat tcctcggatc tccgcgccaa
     1441 gggccccggc aagcggctcg attatcatcg ggcaactata tgacaaaacg cattgcgatc
     1501 gtcggcgcgg gagcgctcgg cggctatgtc ggcggcaccc tcgcccacct cggcttcgat
     1561 atcacgctga tcgatccctg gcccgagcac gtggagatca ttcgcagccg cgggctcgag
     1621 cttgacgggg tgactccgga ggagaaattt gcagtgaggg aagcgaaaac tctgcatgtc
     1681 acggaggttc aatcgctcgc caagacaccg atcgatgtag ctatggtggc ggtgaagtcg
     1741 tacgacaccc attgggccac ggcgctgatc gcgccgtacc tctcggagcg cgggtacgtg
     1801 gtttcgctgc agaactgcat gaacgaggag acgattgctg gcgtagtggg ctgggggcgg
     1861 acggtgggtg ccgtggcctc gttcatctcg gtggacctgt acgaggccgg ccgcatccgc
     1921 cgcacggttc ccaagggcgg cgacaagcac accgtcttcc gcatcggcga gccgcacggt
     1981 gcaatcacgc cgcgcgtcga ggagctggtc gagtggttcc gcggcatcga cagttccaag
     2041 gtcacgacca atctctgggg ggagcgctgg tcgaagctcg ttcagaacgc catgggcaac
     2101 ggggtaacgg cggcaaccgg actgacctcg ggcgcctgcc tgcaccaccc ggcgatccgg
     2161 cgcttccaga tcgagctcgc ggccgaggcg gtgcgcgtcg gccaagcgct cggctaccag
     2221 ctcgaaaaga tccgcggcgc tgcgcccgag ctctacgtac gcgccgcgga cggagactcg
     2281 caagccctgt cggagctgga agctctcatc gtgccgaaag ccggctccaa tccccgcgcg
     2341 gatatccagc gtccttccat ggcgcaggac atcctgaagg gaaggcgcac cgagatcgac
     2401 gcgatgaacg gctacatcgc gcgcaagggc gctgaggtcg gcgtgcctgc gccgagtcac
     2461 aagaagctcg ccgaaatcgt cacgcgcatc gagcgcggcg aggtcaagcc ctcgccttcg
     2521 ttgctttcaa tttgagccga aggtcttcgg ggtacatcgt gaaagcgagg tgctcgcgcg
     2581 cctcgccgcc gtccggggtg ccgacgctct cgatctcgaa attgttgagg agcatcgtca
     2641 ttaccatttt catctcgagg atcgcgaggt aacgcccggg acacaggcgc ggcccggcgc
     2701 cgaaaggcat ggatacgcgc ttcggtgacg ccgcggtcgc gcccgagagc cagcgtgcgg
     2761 gatcgaacgc ccgcgcgtcg gggaagcgcc gctcgtcgat cgcgcccgcg cgcatcaggc
     2821 acatgaggat cgttcccttg gggacttcga taccggcgat caccgtgtcc cgtgtgggct
     2881 gcaggaggag gatcggcgcc accggcttga gccgcatggt ttcgttgatg cacgcatcga
     2941 cgaagggcag cgccgtcatg ctttcatagt gatggacgtc cttgccgagg acttcttcgc
     3001 gcgccctgcg cagcgcttcg ggatgccggt acaggaggta gatcatccag gcgagggtgt
     3061 tggcagtcgt gtcctcgccc gcgagcagca tggtcagcac gttccc
//
LOCUS       DASTPD010000233         6861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1350247, whole genome shotgun sequence.
ACCESSION   DASTPD010000233 DASTPD010000000
VERSION     DASTPD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350247"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFR57_07725"
     CDS             <1..747
                     /locus_tag="VFR57_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0259288.1"
                     /translation="ARAGKAIFCEKPIDLSLSKVDSCLQEVEKAGVPMFVGFNRRFDP
                     SFSALKARLDAGEIGAVEQVVISSRDPGPPPLSYIKVSGGMFRDMTIHDFDMARWLLG
                     EEPVEVFAMGECLVDRKIGAAGDIDSAMVLLRTGSDRMCHINNSRRAAYGYDQRIEVF
                     GARGMLRAENLGETTVERFGPRGTVADRPLDFFLERYADAYRAEMAAFVRALTQSTPM
                     PVGAKDGRQALVLAEAALQSHKKKQAIRIG"
     gene            740..1732
                     /gene="iolG"
                     /locus_tag="VFR57_07730"
     CDS             740..1732
                     /gene="iolG"
                     /locus_tag="VFR57_07730"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEU0259289.1"
                     /translation="MVNFALFGAGRIGRMHAENLAQRSDARLLYVVDTKPEAARALAE
                     PLGARQAAAEEAFSDPQVHAVLIASSTGSHADLSIAAARAGKAIFCEKPVDLTLPKVD
                     LCIKEVRKARVPMFVGFNRRFDPSFRELKKRLDAGVIGKLEQVIITNRDPGLPELRFL
                     ASSGGLFLDFTIHDFDMARWLLGEEPVEVFAWGAVLVDPRVRTEGKDIDTAMLLLRTA
                     SGKMCHINNTRRAVYGYDQRIEVLGAKGMLRAANLAPTSVERFGAEATCVDNPWPNFQ
                     TRYAAAYAAELSSFIRGVEARKPVEVGPEDSRQVLVLAEAALKSSQTGRPVRLK"
     gene            1766..2815
                     /locus_tag="VFR57_07735"
     CDS             1766..2815
                     /locus_tag="VFR57_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0259290.1"
                     /translation="MDQAERKDTAQHALVYVAIAVAVVVILVLLWYAIDVVLLAFVGV
                     LAAILLRAPADWLAQRFGWSEGWSLALVGAVLLGVVIAGGVLFGRGILSQALALVDRI
                     PEIIEGFRKELGKSELGGRLVAFADMSGMFSSGGVQVLGRGLGLIGSTFGVVANVLIV
                     VFFAVFMAAQPRMYVEGALHLVARKKRDRVREVLYEIGRVLRRWLVGQTALAVCVAIL
                     TGAGLVLLGAPFPFALALLAGLMEFVPYIGPFLAAIPAVMVAFANGPELALYVALLFI
                     GVQLVESYVLAPLVQQRAVHLPPGAILFAQVLMGAIVGALGVAVATPLAAAVMVAVGM
                     LYVEDALGDQNATPR"
     gene            complement(2812..3075)
                     /locus_tag="VFR57_07740"
     CDS             complement(2812..3075)
                     /locus_tag="VFR57_07740"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEU0259291.1"
                     /translation="MSRACAALALTLMAGCAAFSPTEADCRGMNWHARGYADGFGGHP
                     PQDLRLDAACRRVGVAVDANRYLAGWRDGHDEWYRIMGSMGLD"
     gene            complement(3072..3338)
                     /locus_tag="VFR57_07745"
     CDS             complement(3072..3338)
                     /locus_tag="VFR57_07745"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEU0259292.1"
                     /translation="MRSTVFLLACVLAGCALFPLEEADCVGVDWRKRGYADGYAGHPQ
                     QFLRLQQECRRFGVEVNETEYFAGWKDGYDEWYRLIGSMDRIRR"
     gene            complement(3377..3691)
                     /locus_tag="VFR57_07750"
     CDS             complement(3377..3691)
                     /locus_tag="VFR57_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259293.1"
                     /translation="MIGVFGSPAAAEQARGVLLEAGVPEIRIALSATLTDDGIGAEAP
                     GQSFENQPGQPRADTKVARYGEAVRTGACVLSVFTRSEEEKQHLEQLLLRNRAHHIAI
                     RR"
     gene            3867..5858
                     /locus_tag="VFR57_07755"
     CDS             3867..5858
                     /locus_tag="VFR57_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0259294.1"
                     /translation="MSPTIPKRVIIGFSLGFAILAVNALIAYQTIISLKQATRSVEEG
                     LQVAELLRGVRSAVADSEAGQRSYIISERKAYLEHSGELLRVADRRLSDIRALIGDDA
                     VELKRIDSLQSSIAARTAEFWNALALLESGDRRAALKAISTEESRRNLDDTYELFRQF
                     RAKQGELFMQRTRQLQEHSRFSLATQYIATFFGLVFLGLIYYLVYREITVRRQTEEKL
                     KIVATHDALTALPNRTLLHERLSQALARAQRHGRPLAVLLVDLDRFKHVNDALGHGAG
                     DTLLQVAARRIYDCLRETDTMARQGGDEFVVLMDELSDRERITRISKGILDATVEPFV
                     IEGQEIHVSASIGISVYPDDGRTLLRNADIALYRAKGKGGNNYQFYSAQIDNYSAERL
                     ALESGLRRALERDELRLHYQPKVSLAAGRICGMEALLRWQHPQMGPVAPDRFIPIAEE
                     SGLIQPIGAWVLKTVCMQNRAWRQQGMPRFPIAVNLSPRQFAEESLLHDIKSALADSG
                     LEGSDLELEITESMVMNDPEQAVTTLRQLKDLGIRVAIDDFGTGYSSLAYLKRFPIDS
                     VKVDRSFIEDIPQDVDSMAIVQAIIAMAHGLRLKVVAEGVESEVQVSFLRAQGCDEIQ
                     GHYFSAAGPASEISGIMRKTLRPGTTIFLSEHRRRRASA"
BASE COUNT         1221 a         2217 c         2201 g         1222 t
ORIGIN      
        1 gcgcgcgccg gcaaggcgat cttctgcgag aagccgatcg acctttcgct ttcgaaggtg
       61 gactcgtgcc ttcaggaagt tgagaaggcc ggcgtgccga tgttcgtcgg cttcaaccgc
      121 cgcttcgacc cgagcttcag cgcgcttaag gctcgcctcg acgccggcga gatcggcgcg
      181 gtcgagcaag tggtgatctc gagccgcgac ccggggccgc cgccgctttc gtacatcaag
      241 gtctcgggcg gcatgttccg cgacatgacg atccacgact tcgacatggc gcgctggctg
      301 ctcggcgagg agccggtcga ggtgttcgcc atgggagagt gcctggtcga tcgcaagatc
      361 ggcgccgccg gggacatcga ctccgcgatg gtgctgctgc gcaccgggtc agaccggatg
      421 tgccacatca acaattcgcg ccgcgcggcg tatggctacg accagcgcat cgaggtgttc
      481 ggcgctagag gaatgctgcg cgcggagaat ctcggcgaga ccacggtcga gcgcttcggc
      541 ccgcgcggca cggtcgccga ccggccgctc gatttcttcc tcgagcgtta cgcggacgcc
      601 tatcgcgccg agatggccgc attcgtccgt gcccttacgc aaagcacgcc catgcctgtc
      661 ggtgcgaaag acggccgcca ggccctggtt ctcgccgaag ccgctctgca gtcgcacaag
      721 aagaagcagg caatccgcat tggttaactt cgccctgttc ggcgccggac gcatcgggcg
      781 catgcatgcc gagaatctcg cgcagcgcag cgacgcgagg ctgctctacg tcgtcgacac
      841 caagcccgag gcggcgcgtg cgctcgccga gccgctcggc gcgaggcagg ccgccgcgga
      901 agaggcgttc tccgatcccc aagtccatgc cgtcctgatc gcgtcgtcga ccggcagcca
      961 cgccgatctc tcgatcgccg cggcgcgcgc cggcaaggcc atcttctgcg agaaaccggt
     1021 ggatctcacg ctgccgaagg tggacctgtg catcaaggaa gtaaggaaag cgcgggtgcc
     1081 gatgttcgtc ggcttcaacc ggcgcttcga cccgagcttc cgcgagctca agaagcgcct
     1141 tgatgccggc gtgatcggca agctggaaca ggtgatcatc accaaccgcg acccggggct
     1201 gcccgagctg cgcttcctcg cgtcctcggg cgggcttttc ctcgacttca ccatccacga
     1261 cttcgacatg gcgcgctggc tgctcggcga ggagccggtc gaagtgttcg cctggggcgc
     1321 ggtgctggtc gatccgcgcg tgcgcaccga ggggaaagac atcgacaccg cgatgcttct
     1381 gctgcgtacc gcgagcggca agatgtgcca catcaacaac acgcgccgcg cggtctacgg
     1441 ctacgatcag cgcatcgagg tgctcggcgc gaaaggcatg ctgcgcgcag cgaaccttgc
     1501 gccaaccagc gtcgagcgct tcggcgccga ggcgacctgc gtggacaatc cctggcccaa
     1561 tttccagacg cgctatgccg cggcctacgc tgcagagctc tcatcgttca tccgaggcgt
     1621 cgaggcgcgc aagccggtcg aggtcgggcc cgaggacagc cggcaggtgc tggtcctcgc
     1681 cgaggctgcg ctgaaatcca gccagaccgg tagaccggtc agattgaaat agaacctcga
     1741 aggggcggcc ggggtccatc aaaccatgga ccaggccgag cgcaaggaca ctgcccagca
     1801 cgcgctggtt tatgtcgcga tcgcggtggc cgttgtagtg atcctcgtcc ttctgtggta
     1861 cgcgatcgac gtagtcctgt tggcgttcgt cggcgtgctt gccgcgatcc tgctgcgcgc
     1921 gccggccgac tggctcgcgc agcgcttcgg ctggagcgaa ggctggtcgc tcgcgctggt
     1981 cggcgcggtg cttctcgggg tagtcatcgc cggcggtgtg ctcttcggcc gcggcatcct
     2041 gtcgcaggcg ctcgcactcg tcgatcgcat cccggagatc atcgagggct tcaggaaaga
     2101 gctcggcaag agcgagctcg gcggccggct cgtcgcgttc gcggatatga gcggcatgtt
     2161 ctcgagcggc ggcgtccagg tcctcggccg cggactcggg ctgatcggat cgactttcgg
     2221 cgtggtggcc aacgtgctga tcgtggtctt cttcgcggtg ttcatggccg cgcagccgcg
     2281 catgtacgtc gaaggcgcgc tgcatctagt cgcgcgcaag aaacgcgacc gcgtgcgcga
     2341 ggtcctctac gaaatcgggc gcgtgctgcg ccgctggctc gtggggcaga ccgcgctcgc
     2401 cgtctgcgtc gctatcctca ctggcgccgg cctggtacta ctcggtgcgc cgtttccttt
     2461 cgcgctcgcg ctgctcgccg gcctgatgga gttcgtgccg tacatcggcc cgttcctcgc
     2521 cgcgataccc gcggtgatgg tagctttcgc aaacgggcct gagctcgcgc tttacgtcgc
     2581 gctgctcttc atcggcgtgc agctcgtcga gagctatgtg ctcgcgccgc tcgtgcagca
     2641 gcgggcggtg cacctgccgc cgggggcgat cctcttcgca caggtgctga tgggcgccat
     2701 cgtcggcgcg cttggcgtcg cggtcgcgac cccgcttgcg gcggcggtca tggtggcggt
     2761 tggaatgctc tacgtcgagg acgcgctcgg cgaccagaac gccacgcccc gctaatccag
     2821 ccccatcgag cccatgatcc ggtaccactc atcgtggcca tcacgccaac cggcgaggta
     2881 acggttcgcg tccactgcga ctccgacgcg gcggcacgcc gcgtcgagcc tcaaatcctg
     2941 cggcggatgg ccgccgaagc cgtcggcgta gccgcgcgcg tgccagttca tgccacggca
     3001 gtcggcctcg gtgggactga acgcggcgca gcccgccatc agcgtaagcg ccagagctgc
     3061 gcaggctcga ctcatcgacg tatgcggtcc atcgacccga tcagccgata ccactcgtcg
     3121 taaccgtcct tccagccggc gaaatattcg gtctcattga cctcgactcc gaaacggcgg
     3181 cattcctgct gcaggcggag gaactgctgc ggatggccgg cgtagccgtc ggcgtagccc
     3241 cttttgcgcc agtcgacgcc cacgcagtcg gcttcctcga gcggaaaaag cgcgcagccc
     3301 gcgagcacgc aggcaagcaa aaaaactgta cttcgcatga ccggtaaaca gcctggcccg
     3361 ggcgaggatt ccgtgcttag cgccgaattg cgatgtggtg cgcccggttt cgtagcaata
     3421 gctgctcgag gtgctgcttc tcttcttccg agcgggtaaa tacgctcagc acgcaagcgc
     3481 cggtgcgaac ggcttcgccg taccgggcaa ccttagtgtc ggcgcggggc tggccgggct
     3541 ggttttcgaa cgactgcccc ggcgcctcgg cgccgatccc atcgtcggtc agggtcgcgc
     3601 tgagcgcgat gcgaatctcg ggtacccccg cctcaagcag cacgcctctt gcctgctcgg
     3661 cggcggccgg gctgccgaag acgccgatca cggtttgtga cccgagaact cccattcccc
     3721 gtcccgtgca acatccgtgc cgtggcgtct aagcaacgac atctttagcc atgtcagcaa
     3781 tcgtcagtgt gacaaattat gcgtgtgtaa ggctcgcccc gggaacatag gatgcgcatc
     3841 atcgcgcact gcacggaggt cgttcaatga gcccaacgat tcccaagcga gtcatcatcg
     3901 gattcagcct ggggttcgcg atcctcgccg ttaatgccct gattgcctat caaaccatca
     3961 tttcgctcaa gcaggcgacc cgttccgttg aggagggctt gcaggttgcg gaactgctga
     4021 ggggcgtacg ctccgccgtg gcagattccg aagcggggca gcgcagctac atcatcagcg
     4081 agaggaaggc gtacctagag cattccggcg aacttcttcg ggttgccgat cgccggctga
     4141 gcgacatccg cgcactgatc ggcgacgacg ctgtcgagct gaagaggatc gattcactgc
     4201 agtcatcgat tgcggcacgc accgctgaat tctggaatgc gctcgcgctg ctggagagcg
     4261 gcgatagacg ggccgccctc aaggccattt cgaccgaaga gtccaggcga aacctggacg
     4321 atacctacga gcttttcaga cagtttaggg cgaagcaggg cgagctgttc atgcagcgga
     4381 cccgacagct tcaagaacat tcccgcttca gcctcgcgac gcagtacatc gccacctttt
     4441 tcggcctggt ctttctcggc ttgatctatt acttggtcta ccgggaaatc acggtgcgca
     4501 ggcagacgga agaaaagctc aagatcgttg ccactcatga cgcgctgacg gcgctgccca
     4561 atcgcacctt gctgcacgag cgcttgtcgc aggcgcttgc gagggcgcag cgccacggcc
     4621 ggccgctcgc cgtcctcctc gttgatctgg accgcttcaa gcacgtgaac gatgcgctgg
     4681 gccacggggc cggcgacacg ctgcttcagg ttgcggcgcg gcggatatac gactgcctcc
     4741 gggagaccga tacgatggcc cggcaggggg gcgatgagtt cgtggtgcta atggacgagc
     4801 tctcggatcg cgaacggatt acacgcatat cgaaggggat cctggatgcc acggtcgaac
     4861 ccttcgtcat cgaagggcag gaaatccatg tgagcgccag catcggcatc agtgtctacc
     4921 cggatgacgg caggaccctg ctgaggaacg cggacatcgc gctgtaccgc gccaagggga
     4981 agggaggcaa caactaccag ttctattcgg cgcagatcga caactattcc gccgagcgcc
     5041 tggcgctgga atccgggctg cgccgcgcgc tcgagcgcga tgaattgagg ctgcactatc
     5101 agcccaaggt gagcctcgcc gccgggcgca tctgcggaat ggaggcgctg ctgcgctggc
     5161 agcatccgca gatggggccg gtcgcgccgg accggttcat tccgatcgcc gaggaaagcg
     5221 gcctcataca gccgatcggc gcctgggtgc tgaagaccgt gtgcatgcag aaccgggcgt
     5281 ggcggcagca ggggatgccg cgctttccca tcgcggtaaa cctgtcgccc cgccagttcg
     5341 ccgaagagag ccttctccat gacatcaagt cagcgctcgc cgattccggg ctggaagggt
     5401 cggacctgga actcgagatc accgagagca tggtgatgaa tgaccccgag caggcggtca
     5461 ccaccctgcg tcagctgaag gacctgggaa tccgcgtggc gattgacgat ttcggcaccg
     5521 gctattcatc gctcgcgtac ctcaagcgct tcccgattga cagcgtgaaa gtggatcgtt
     5581 ccttcatcga agatatcccg caggacgtgg acagcatggc tatcgttcag gcgatcatcg
     5641 ccatggcgca tggcctgcgc ctcaaagtgg tcgctgaggg agttgaaagc gaagtgcagg
     5701 ttagcttctt gcgcgcccaa gggtgcgatg agatacaagg gcactatttc agcgcggcgg
     5761 ggcccgcaag cgaaatttcc ggcatcatgc gcaagacctt gcggcccggc actaccattt
     5821 tcctttcgga gcatcgtcgt cgcagggcat cggcctaggc tgtatttttc aaactcgggt
     5881 ccttctcggc cggcacataa agcaagcgag cggcaccaag cggccgccta acagcgggac
     5941 tactgcggcg gccgcagaga gccgccacag gcaccactag agcggctcat tgatttgatc
     6001 acctgcgagc aaaaaaagtc gaccgctaaa gtccgcgcgc tcttcgccaa gactcgcaaa
     6061 ccggggacat cgaaaatcta gtcggctcgc tcgcgcgtca gaaccacagt gcccgaacga
     6121 ggagcgccac agtactaact gaccacacca acggcagggt ccacggatag aggcgatcgg
     6181 tggccggccg caggccatct tgaccgtccc gctgcgctgg aaacagactc gcttcaaggc
     6241 tcacgcggta atgatgctcc caagcatggc attcaaagaa caactcttgt gttcgtcggc
     6301 gcgcggcgtg acaaatatcc ggacagccgc acacgacatt tcaagtacga gcgcgagtac
     6361 gacgcgcgga taagaggcgc ccgaggttgt gggtcagcgc ggcccactga ggggtcaggc
     6421 gtctagcgcc gagcgctcgc gagcactacg tagatcacct cgacatctcc ttcgaggcgc
     6481 ccctcgaagt caaccgcaat ccgccgcgat cgacccgagt cgcccagttc gcgggctgcc
     6541 gtctcgtcgt ccttgcccct caagtacaaa acgcggactc ttcgcttgcc acgccggtta
     6601 gcgagcggca gcgcgccacc ccgtgcagat ttggtttcca tagttagccg ctcctctgtg
     6661 acaggttcgg ctttaagcgc ctgctctgtg gatgacgcta gataagccac cagacgaccg
     6721 ccgcccagaa cggtgccgac agcatcacgg ctaccatcac gcctcgggcc ggggccaagg
     6781 ggtcggtgtc gtcgtactcc tggacgagct ttagtacttc aagttcagtt tcgggctggc
     6841 tcttcaccga cggcaagcgg c
//
LOCUS       DASTPD010000234         6861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_941821, whole genome shotgun sequence.
ACCESSION   DASTPD010000234 DASTPD010000000
VERSION     DASTPD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941821"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFR57_07760"
     CDS             <1..752
                     /locus_tag="VFR57_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259295.1"
                     /translation="GDSGGFCSPVASAPPVQESEDLVNLILSFIHPSKPSRQVGPVTS
                     VRIDAESMHDGTGGKLIARHREHHWEVEGERFFRVDMTTRVRIHFERARPAADAPTKS
                     RSFGPYERFSAVDGIAYADDRVFAFVDARVGDWYCYGDGRHWSVMVVADASRRAAKDT
                     LRTVIGLAPFLAGVIGLWEGAKLLYLGRAQSIRDRLETFANEPRTLDPSRVSAVTWEC
                     HPDPAAREAELYAEYLDGRQTVELARSRRDQ"
     gene            749..1372
                     /locus_tag="VFR57_07765"
     CDS             749..1372
                     /locus_tag="VFR57_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU0259296.1"
                     /translation="MKPLNAIAGLIAFVVLSLGTPATAQEAVEVGKPAPGFKVSDAEG
                     RVRQLPEFAGKPVVLEWTSPSCPFVRAQYQSGVMQELQKMAAQQGAVWLTVLSTHPSR
                     RDYLPPEKASALHRGRGGASSALLMDADGTLGKAYGAVVTPQIFIIGADGKLVYAGAT
                     GDKSTTNAKEVRSSRNFIREALADLAAGRKIATPSSRPFGCTVAYEG"
     gene            1426..1501
                     /locus_tag="VFR57_07770"
     tRNA            1426..1501
                     /locus_tag="VFR57_07770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1459..1461,aa:Arg,seq:cct)
     gene            complement(1508..1891)
                     /locus_tag="VFR57_07775"
     CDS             complement(1508..1891)
                     /locus_tag="VFR57_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0259297.1"
                     /translation="MPRRLRVLVVDDNPDQVKSVLLLLETEGYEARGVYNAHSILSEV
                     LEFDPDVVIMDIKMPERSGWEAASELRQRVTNLRLLLIAVSGHYTRGVDKVLSEMSGF
                     DHVLTKPIDPATLLGLIAKHTSQVA"
     gene            complement(1946..3460)
                     /locus_tag="VFR57_07780"
     CDS             complement(1946..3460)
                     /locus_tag="VFR57_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0259298.1"
                     /translation="MFELLGAGLADMMQVKYLVPLFVGTLVGVVGGALPGITITMTVI
                     VVLPFTFGLEPLQGLACMIGVYVGGETGGLITSCLLGIPGKPSAVATTFDGFPMARKG
                     EPGRALWIGIWASIFGGLLGAIVLVAATGPLAQLALQFGPWEYFALFVLALSMVAGLA
                     ESSLLKGLLSGALGCVVTILGNDPVMGQPRLTLGIGWLEGGIPFLPVLIGVFAFAQIM
                     SDVERFSRTPEAVNTAVNLVVSHAKVLAEILARPFLLLWSTFIGLVIGVLPAIGGSAA
                     NMLAYDQAKKFSKRPEKFGTGIPEGIIASEASNNANVAGSLVTIMAFGIPGDAVTAVM
                     LGALTIHGIQSGPLFISQNAPLAYGMFAAYILAHPLVLILLFLGVRWVLRIVIVPKSI
                     LFPVVLVLCVVGAYALGNTMATVYVLLASGVFGYLMVKFGFPLAPFILGVILGDQIEI
                     NLVRSLMTDSDLWLFVTRPISALLLAMAVGSIVLSVWQHRRMQKRAATEGAADF"
     gene            complement(3460..3924)
                     /locus_tag="VFR57_07785"
     CDS             complement(3460..3924)
                     /locus_tag="VFR57_07785"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0259299.1"
                     /translation="MKLSKDGWAGLAVAAASLFLFWLTLGLKDNPLVPIGPGYYPRIV
                     LGVTAAFALALLLWDALHPKPSPPRERLNYVLVLEMFAVFGLYVGALPYVGFRISTFF
                     YIAATNALLDRPERGKGWLRVLAVALITTIVVYYAFERYLTVLLPRGRWTNF"
     gene            complement(3921..4859)
                     /locus_tag="VFR57_07790"
     CDS             complement(3921..4859)
                     /locus_tag="VFR57_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259300.1"
                     /translation="MKKLLIAAALAATSWTVHAAYPERPVRLIVPWAAGGDTDNIFRP
                     FAQVWQKHLGQTVVIANVGGASGTRGAKEAKDAPPDGYTLYAVHDYIHSTYWAGVADV
                     QYADFEPICLISSTPSVLTASPKTPWKTLKELLDDAKKRPGQITVGATLASTSHFFPA
                     LIEKASGAKFKYVSYEGLAPRMNAILGGHVDLTDSNLTQKGKVEAGQLKFLAIATEKR
                     HPEMPSVPTLKELGVNVVYDVNRGIMVPKGTPAEVIGKLGSSCAAAAKEPEFAQAMKL
                     QGTDVRYMDRNAYASWLKQNDDLNRNLAKDLGLLKR"
     gene            complement(4905..5933)
                     /locus_tag="VFR57_07795"
     CDS             complement(4905..5933)
                     /locus_tag="VFR57_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0259301.1"
                     /translation="MKRILLLGAGHAHLVVLRSLVQEPLHRARITLVTPHAKQVYSGM
                     LPGILAGHYRRADAEVDVAALAERAYVELEQGLVERLDLKQRMVLTKGGKSFAYDVAS
                     LNVGSQIARGFRGAQLALAVKPYEEFLARLRMAERIAVVGGGVAGAEIAMALRYRGAQ
                     VTLYAENPVLSPRVAKQLGKRKVDYRPGMAVTAIEPGLVVVSGSARQDFELALLATGA
                     TAMPWLRSSGLATDERGFALVGATLQSISHPEVFAVGDCATLRETPHPKSGVYAVRHG
                     ETLVENLRRIVSDAAPAAYVPQEKALALVTCGARYAIAERGDWGAQGFSLWWWKDRID
                     RRWVRSFK"
     gene            complement(5930..>6861)
                     /locus_tag="VFR57_07800"
     CDS             complement(5930..>6861)
                     /locus_tag="VFR57_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0259302.1"
                     /translation="ALLPSWAFGVPILGYRGKFDAEKAYYLLEKYGVRNSFLFPTALK
                     LMMKAVPSPRAKFDLGLRSVMSAGESVGVTVFEWAREELGVTINEMFGQTEINYVVGN
                     CQSAWPAKPGSIGRPYPGHRVAVIDEQGKELAPGELGEVAVNRFFNGQRDPVFFLEYW
                     KNESATREKFIGDWGCTGDQAKRDEDGYLWYQGRSDDVIKSSGYRIGPAEIESCLVKH
                     PSVLNAAVIGKPDATRGAIVKAFVVLQPGIRPGEKLVEDIQNHVRGRLAPYEYPREIE
                     FLDALPMTTTGKVQRKELRKLEQGKTPSKLSEA"
BASE COUNT         1237 a         2199 c         2247 g         1178 t
ORIGIN      
        1 tcggcgatag cggcggcttt tgttcccccg tagcatcagc accgccggta caagaatcgg
       61 aagacctagt gaacctaatc ctttccttca ttcatccgag taaacccagc cgccaagtcg
      121 gaccggtcac gtcagtgcgc attgacgccg aaagcatgca cgacggcacg ggcgggaaat
      181 tgatcgcacg gcatcgcgag catcactggg aagtggaggg cgagcgcttc tttagggtcg
      241 acatgacgac ccgggtcaga attcatttcg aacgcgcgcg gccggctgcg gatgccccga
      301 ccaaatcgcg cagcttcggt ccttacgaaa ggttctcggc ggtcgacggc atcgcctatg
      361 cggatgaccg cgtgttcgcg ttcgtcgatg cacgggtcgg cgactggtat tgctacggcg
      421 atggtcggca ctggtcggtc atggtggtgg ccgatgccag tcgccgcgct gcgaaggata
      481 ccctgcgcac ggtgattggt cttgcgccgt tcctcgccgg ggtgatcggg ctttgggaag
      541 gcgccaagct tctatacctt ggtcgagcgc aatccatacg cgaccgtctc gaaacatttg
      601 ccaacgaacc gaggacgctc gatccgagcc gtgtgtcggc tgtgacctgg gaatgccatc
      661 ctgatcccgc tgcccgcgaa gcggagctct acgctgaata ccttgacggc cgacaaaccg
      721 tcgagctcgc gcgaagtagg agggaccaat gaagccactg aatgccattg ccggattgat
      781 tgcgttcgta gttctcagcc tcggcacgcc cgctaccgcg caagaagctg ttgaggtggg
      841 aaagcccgct cccggcttta aggtgtcgga tgccgaagga cgcgtgaggc agctccccga
      901 gttcgcgggc aagccggtcg tcctggagtg gacgagcccg tcctgcccct tcgtgcgcgc
      961 gcagtatcag agcggcgtca tgcaggagct gcagaaaatg gccgctcagc agggagccgt
     1021 ctggctgacc gttttatcga cccacccgtc tcgccgcgac tatctacccc ctgagaaggc
     1081 gagcgccctt caccgtggtc gcggcggcgc gtcgagcgcc ctgctcatgg acgcggacgg
     1141 aacattaggc aaggcgtatg gcgccgtcgt cacgccgcag atcttcatca tcggggccga
     1201 cggcaagctg gtgtacgccg gcgctacggg agacaagtcg acgaccaacg cgaaagaagt
     1261 gcgctcctcg cgcaatttca ttcgcgaagc gctggccgac ctcgccgcgg gtcgaaagat
     1321 cgcgacgccg agcagccggc cgttcggctg cactgtcgcc tacgaaggtt aggcgggcgc
     1381 cgaccagcgc ttcaccatga ggcccgccgc tagaatggcg gccgggcccc ggtagctcag
     1441 tggatagagc agtcccctcc taagggacag gtcgcacgtt cgattcgtgt ccggggtgcc
     1501 aggcctttca ggctacctga gaggtgtgct tagcgataag cccgagaagc gtagccggat
     1561 cgatcggctt cgtcagcacg tggtcgaagc cgctcatttc cgaaaggact ttgtctacac
     1621 ccctggtgta atggccgctc accgcaatga gtaacagccg caggttcgtg actcgttggc
     1681 gcagctcgct cgccgcctcc cagccgcttc tttccggcat cttgatgtcc atgatcacga
     1741 catccggatc gaattccagg acctctgaaa ggatgctgtg agcgttgtag acccctctgg
     1801 cctcgtaccc ctcggtctcg agaagaagga gcacggactt cacctgatca gggttgtcgt
     1861 cgacaacaag gacacgcagg cgacgcggca tggcggctat tcattccacg ttttacgctc
     1921 ttcgtctatc gtgccacgtc tttatctaga aatccgcggc gccttcggtc gcggccctct
     1981 tttgcatccg ccggtgctgc cagaccgaca gtacgatgga tccgacggcc atggcgagga
     2041 gcagcgccga gatcggccgg gtaacgaaca gccacaagtc ggaatcggtc atcagcgagc
     2101 gcacgaggtt gatctcgatc tggtcgccga ggatcacgcc caggatgaac ggcgcgagcg
     2161 ggaagccgaa cttgaccatc aggtagccga agacgccgct tgcgaggagc acatagacgg
     2221 tcgccatggt gttgccgagc gcataagcac cgacgacgca gagcacgagg accacgggaa
     2281 agaggatcga tttcggcacg atgacgatcc ggagcaccca gcgcacgcca aggaaaagca
     2341 ggatgaggac gagcgggtgc gcgaggatgt aggcggcgaa catgccgtag gcgagcggcg
     2401 cgttctgcga gatgaagagc gggccggact ggatgccgtg gatggtgagc gcgccgagca
     2461 tcaccgcggt gaccgcgtcg cccggaatgc cgaacgccat gatggtgacg agcgaacccg
     2521 ccacgttggc gttgttcgac gcttccgagg cgatgatgcc ctcgggaatg ccggtgccga
     2581 atttttccgg gcgcttggag aacttcttcg cctggtcgta ggcgagcatg ttggccgcgc
     2641 tgccgccgat cgcggggagc acgccgatca ccaggccgat gaacgtggac cacagaagca
     2701 ggaaagggcg cgcgaggatt tcagccagca ctttggcgtg actcaccacc aggttgaccg
     2761 cggtgttcac ggcttccggc gttcgtgaga agcgttccac gtcgctcatg atctgcgcga
     2821 acgcgaacac gccgatcagc acgggcagaa acggaatgcc gccctccagc cagccaatac
     2881 cgagcgtaag gcgcggctga cccatcaccg ggtcgttgcc gaggatcgtg accacgcagc
     2941 cgagcgcgcc ggagagcagg cccttcagga gcgagctctc cgcgagccct gcgaccatcg
     3001 acagcgcgag cacgaaaagc gcgaagtact cccacgggcc gaactgcagc gcgagctgcg
     3061 cgagcggccc ggtcgccgcg acgaggacga tagccccgag cagtccgccg aagatcgagg
     3121 cccagatccc gatccagagc gccctgcccg gctcgccttt gcgcgccatc gggaagccgt
     3181 caaaggtcgt cgccaccgcc gagggcttgc ccgggatgcc gagcaggcat gaggtgatga
     3241 ggccgccggt ctcgccgccg acgtagacgc cgatcatgca tgcgagccct tgcagcggct
     3301 cgaggccgaa ggtgaacggc aggaccacga tcacggtcat ggtgatcgtg atccccggca
     3361 gcgcgccgcc gaccacgccg accagcgtgc cgacgaaaag cggcacaagg tacttaacct
     3421 gcatcatgtc cgcgaggccg gcgcctagga gctcgaacat cagaagttcg tccagcgccc
     3481 gcgcggcagg agcacggtca ggtagcgctc gaaggcgtag tacacgacga tggtggtgat
     3541 cagcgcgacg gcgagcacac gcagccagcc ctttcccctt tccggtcggt cgaggagcgc
     3601 gttcgtcgcg gcgatataga agaaggtgga gatgcgaaaa ccgacgtagg gaagcgcgcc
     3661 cacgtagagc ccgaacacgg cgaacatctc gagcacgagg acatagttaa gccgctcgcg
     3721 cggcgggctc ggcttggggt gcagcgcgtc ccaaagcagg agcgccagcg caaaggcggc
     3781 ggtgacgccg agcacgatgc gcgggtagta gcccggcccg atcggaacga gcggattgtc
     3841 cttgaggccg agcgtgagcc agaaaagaaa aaggctcgcc gcagccacgg cgagccccgc
     3901 ccagccgtct ttcgacagct tcaacgtttg agtagaccca ggtcttttgc gaggttgcgg
     3961 ttgaggtcgt cgttctgctt gagccagctt gcgtaagcgt tgcggtccat gtagcgcacg
     4021 tcggtgccct gcagcttcat ggcctgcgcg aactcgggct ccttcgcggc cgccgcgcag
     4081 ctcgaaccga gcttgccgat cacttcggcc ggcgtgcctt tcggcaccat gatgccgcgg
     4141 ttcacgtcgt agaccacgtt gacgcccagc tcctttagcg tgggcacgct cggcatctcg
     4201 gggtggcgct tctcggtggc gatggcgagg aacttgagtt ggccagcctc gaccttgccc
     4261 ttctgcgtga ggttggagtc ggtgaggtcg acgtggccgc cgaggatcgc gttcatgcgc
     4321 ggcgcgaggc cctcgtagga aacgtatttg aacttcgccc cgctcgcttt ctcgatgagc
     4381 gccgggaaga agtggctggt cgaggcgagc gtcgcgccga ccgtgatctg gccggggcgc
     4441 tttttcgcgt cgtcgaggag ctccttcagt gtcttccacg gcgttttggg gctggccgtc
     4501 agcaccgagg gggtcgatga gatgaggcag atcggttcga aatccgcgta ttgcacgtcg
     4561 gcgacacccg cccagtacgt cgaatggatg tagtcgtgca ccgcgtacag cgtgtaaccg
     4621 tcaggcggcg cgtctttcgc ttctttcgct ccgcgcgtgc ccgatgctcc gcccacattc
     4681 gcgatgacga cggtctggcc cagatgcttc tgccacacct gcgcgaacgg gcggaagatg
     4741 ttgtcggtgt cgccgccggc ggcccacggc acgatgagcc gcaccggccg ctcggggtac
     4801 gcggcgtgca cggtccatga ggtcgcggcg agcgcggccg ctatcagcag tttcttcatt
     4861 cagtcctcct ccaaaggagc cgcaatgata cgtcgaagca acgcttattt gaacgaccga
     4921 acccagcggc ggtcgatgcg gtccttccac caccagaggg aaaagccctg cgcgccccag
     4981 tcgccgcgct ccgcgattgc atagcgagcg ccgcacgtga cgagcgcgag cgctttctcc
     5041 tgcggcacgt aggccgcggg cgcggcgtcg ctcacgatgc gccgcaggtt ctcgaccagg
     5101 gtctcaccgt ggcgcaccgc gtacacgccg gatttcggat gcggcgtctc gcgcagcgtc
     5161 gcgcagtcgc cgaccgcgaa gacttcggga tgcgaaatgc tttgcagcgt cgcgccgacg
     5221 agcgcgaagc cgcgctcgtc ggtggcgagc ccggaggagc gcagccaggg catcgccgtc
     5281 gccccggtcg cgagcagcgc gagctcgaaa tcctgccgtg cgctcccgga gaccacgact
     5341 aagccgggct cgatcgcggt caccgccatt ccgggccggt agtccacctt gcgtttgccc
     5401 agctgtttgg ccacgcgcgg cgagagcacc ggattctcgg cgtaaagggt gacctgtgcg
     5461 ccgcgatagc gcagcgccat cgcgatttcc gcgccggcca cgccgccgcc cacgaccgcg
     5521 atgcgctcgg ccatacgcag gcgggcgagg aactcctcgt acggtttaac cgccagcgcg
     5581 agctgcgcgc cgcggaagcc gcgcgcgatc tgtgagccga cgttgagcga tgccacgtcg
     5641 taggcaaagc ttttacctcc tttcgtgagc accatgcgct gcttgagatc cagccgctcg
     5701 accagcccct gctcgagctc cacgtaggct cgctcggcga gcgcggcgac gtcgacctcc
     5761 gcatcggcgc gccggtaatg gccggcaagg atgcctggca acatcccgga gtagacctgt
     5821 ttcgcatgcg gcgtcacgag cgtgatgcgc gcgcggtgaa gcggctcctg cacgaggctg
     5881 cgcaaaacca ccaggtgcgc gtggcccgca ccgagaagca gaatgcgctt cacgcctcgg
     5941 acagcttgga gggtgtcttt ccttgttcga gcttgcgcag ctccttgcgc tggaccttac
     6001 cggtcgtcgt catgggcagc gcatcgagga actcgatctc gcgcgggtac tcgtagggcg
     6061 cgaggcggcc gcgcacgtgg ttctggatgt cctcgacgag cttctcaccg ggccggatgc
     6121 cgggttgcag aactacgaag gccttgacga tggcgccgcg cgtggcgtcg ggcttgccga
     6181 ttaccgcggc gttcagcacc gacgggtgct tgacgagaca gctttcgatc tctgccgggc
     6241 cgatgcggta gcccgagctc ttgatcacgt cgtcggagcg cccctggtac cagagatagc
     6301 cgtcctcgtc gcgctttgcc tggtccccgg tgcagcccca gtcaccgatg aacttctcgc
     6361 gcgtcgcgct ttcgttcttc cagtactcga ggaagaacac cggatcgcgc tgcccgttga
     6421 agaaccggtt caccgcgact tcaccgagct cgcccggcgc caattccttg ccctgctcgt
     6481 caatcacggc gacgcggtgg ccgggatacg gccgcccgat cgaccccggt ttagccggcc
     6541 aagcgctctg gcagttgccc accacgtagt tgatctcggt ctggccgaac atctcattga
     6601 tggtgacgcc gagctcctcc cttgcccatt caaacaccgt cacgccgacc gattcgcccg
     6661 cgctcatgac cgagcgcaag cccaggtcga actttgccct cggcgagggc accgccttca
     6721 tcatcagctt gagcgccgtg ggaaagagga aggaattgcg cactccgtat ttctcgagca
     6781 ggtagtaggc cttctcggcg tcgaacttgc cgcggtagcc gaggatcggg acgccgaacg
     6841 cccaggaggg cagcagggcg t
//
LOCUS       DASTPD010000235         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_534194, whole genome shotgun sequence.
ACCESSION   DASTPD010000235 DASTPD010000000
VERSION     DASTPD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534194"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFR57_07805"
     CDS             complement(<1..609)
                     /locus_tag="VFR57_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0259303.1"
                     /translation="MRVLVVEDDPMIGRAVADGLQGEGHAVDLVRDGAAAELALLHGP
                     YDLAVLDLGLPRKDGFEVLKSLRHARVDVPVLIITARDAVADRIAGLDHGADDYLVKP
                     FDLDEFLARARAVVRRRAGRVSPEIAYGALTLDPVKRAVTFRGARVELSAREFAVLEA
                     LMREPGAVISRTKLEDSVYGWGEEIGSNSVEVHLHHLRRKLDP"
     gene            691..2031
                     /locus_tag="VFR57_07810"
     CDS             691..2031
                     /locus_tag="VFR57_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259304.1"
                     /translation="MRLRILLSTLVLCTVAAAHSAPPREQFVPANFYWVGPYAAGGSG
                     ISGGMLDYLEMLNLRDGGINGVRFTWQKCETEYNNARGVECYERSKRQGPTGATLIHP
                     LSTDITYSLIERATADKIPVVSIGYGRADAADGRVFPYVFPLITTYWDQAAAIVRYVG
                     EQVGGGKANPMQSLKGKKIVLLYHDSAYGKEPIPVLTELAGKYGYALSTIPVPHPGNE
                     QQSQWLQVRQIRPDWVILWGWGVMNPTALKAAARIGYPRNRIVGVWWSGAEEDVLPAG
                     NAAEGYIAAGFNVAGADYPVIREIRKHVYADGGGEMNDASRVGSVYYNRGVVFGIITA
                     EAVRVAQERFGKSKPVTGEQVQWALEHLNIDAKRLKQLGAEGFMPPLKTSCADHEGSG
                     VVRFMRWDGVRWQTITGWMAPMPEDRKLVRQRYADSAMQYAREKGITPRKCPAP"
     gene            complement(2173..2298)
                     /locus_tag="VFR57_07815"
     CDS             complement(2173..2298)
                     /locus_tag="VFR57_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259305.1"
                     /translation="MLRAQWEYAHFVRAVLQFAALAALVLSVLAETRRHALSPFS"
BASE COUNT          484 a          901 c          944 g          433 t
ORIGIN      
        1 cgggtcgagc ttgcgccgca agtggtgcag gtgcacttcg accgagttcg agccgatttc
       61 ctctccccag ccgtacacgc tgtcctcgag cttggttcgt gagatgaccg cgccgggctc
      121 gcgcatcagc gcttcgagca ccgcgaattc gcgcgcggaa agctccacgc gtgcgccgcg
      181 gaaggtcacc gcgcgcttca ccgggtcgag cgtcagcgcg ccataggcga tctctggcga
      241 aacgcggcca gcgcgccggc gcacgacggc gcgcgcgcgt gcgaggaact cgtcaaggtc
      301 gaagggcttc accaggtagt cgtcggcgcc gtggtcgagg ccggcgatgc ggtcggccac
      361 cgcgtcgcgc gcagtgatga tcagcaccgg cacatcgaca cgcgcgtgcc gcaggctctt
      421 cagtacttcg aagccgtcct tgcgcggcaa gccgaggtca agcaccgcga ggtcgtaggg
      481 gccgtggagc agcgcgagct ccgcggcggc gccgtcgcgc accagatcca cggcatggcc
      541 ttcaccttgc agtccatcgg ccactgcacg gccgatcatc gggtcgtctt cgactacgag
      601 gactcgcatg gcacgagtat aggtcgctcc atgtttaagg cgcgcttaag ccttgcgggt
      661 tcaccctttt ctcccattcg cgtgaatgcc atgagactgc ggatccttct ttcaaccctg
      721 gtgctgtgca ctgtcgcggc ggcgcactcg gctcccccca gggagcaatt cgtaccggcg
      781 aacttctact gggtcgggcc ctatgctgcc ggcggctcgg gcatctccgg cggcatgctc
      841 gactacctcg agatgttgaa cctgcgcgac ggtgggatca acggcgtcag gttcacctgg
      901 cagaagtgcg agaccgagta caacaacgcg cgcggggtcg agtgctacga gcgctcgaag
      961 cgccaggggc ccaccggcgc gacgctgatc catccacttt cgaccgacat cacctactcg
     1021 ctgatcgagc gcgccacggc ggacaagatc cccgtcgtct cgatcggcta cggccgcgcc
     1081 gacgccgccg acggacgcgt gtttccgtac gtgtttccgc tcatcaccac ttactgggac
     1141 caagcggcag ctatcgtgcg ctacgtcggc gagcaggtgg gcggggggaa ggcaaacccc
     1201 atgcagtcgc tgaaaggaaa gaagatcgtg ctcctgtacc acgactcggc gtacggcaag
     1261 gagccgatac cggtactcac cgagcttgcc ggcaaatatg gatacgcctt gagcaccatt
     1321 cccgtgccgc atccgggcaa cgagcagcaa tcgcagtggc tgcaagtgcg gcagatccgc
     1381 cccgactggg tgattctctg gggctggggt gtgatgaatc cgaccgcgct caaggcggca
     1441 gcgcgcatcg gctatccacg caaccggatc gtgggcgttt ggtggtcagg cgcggaggag
     1501 gacgtgctgc ccgccggcaa cgcggccgag ggctacatag ccgcgggttt caacgtcgcg
     1561 ggcgcggatt acccggtgat ccgcgagatc cgcaagcacg tctacgccga cggtggcggc
     1621 gagatgaatg atgcctcgcg cgtcggttcg gtgtactaca accgcggcgt tgtcttcggc
     1681 atcatcaccg cggaagcggt gcgcgtcgcg caggagcgct tcggaaaaag caagccggtt
     1741 accggcgagc aagtgcagtg ggcgctcgag cacctgaaca tcgacgccaa gcgcctgaag
     1801 caactgggtg ccgagggctt catgccgccg ctcaagacga gctgcgccga ccacgagggg
     1861 tcgggagtgg tgcgtttcat gcgctgggac ggcgtgcgct ggcagacgat caccggctgg
     1921 atggcgccga tgcccgagga ccgcaagctc gtgcggcaga ggtacgccga ctcggcgatg
     1981 cagtacgcga gggagaaggg catcacgccg cgcaagtgtc cggctccctg atcttcttcg
     2041 acaagctgcg caccatgctg cggcttgccc gcaatgcggg cgccgctgcg catctccgcc
     2101 aggagtgaag ccaccgccgc ggcgcgcgtg ctatggcacg cgcgacgagc tgaagcgcgc
     2161 gcctgagccg gcttaggaaa acggggaaag cgcgtgccgc cgggtctcgg ccaacaccga
     2221 gagcacgagc gcagcgagcg cggcgaactg cagcacggcg cgcacgaaat gcgcatactc
     2281 ccactgtgcg cgcagcactc cccagtcggg cggcagcgta tgccgtgaac tgcgcgatcg
     2341 ccgcgttgac cgggcgccgc gccggcccac cacaacaccg cgtgcgcgcc gatgcccttt
     2401 ggaggtcacc aaggaccagc agccacaagg gctcggagaa atggtcaagc gatcgatcat
     2461 cgggacggtg cgcggtaccg gcgagatcgt gaacgcggtc accgacaccg tggccggcac
     2521 gcgctcggcg ctacctagtg ccgggtaggt tttcgctcac cggctggagc ggttcgccgg
     2581 ggaccggtgg gcgatacggg ggcggcggcg accgctgtga tcggagtgct cctcttctag
     2641 aggccaccga tcacgctttg cattccagcg taagtgagaa gaagcgctgg gggtatccga
     2701 ccgacgcggc atcgggcacc cgggttcccc gatccagtac cgtcggtgtg ccgcggaaga
     2761 gc
//
LOCUS       DASTPD010000236         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_124041, whole genome shotgun sequence.
ACCESSION   DASTPD010000236 DASTPD010000000
VERSION     DASTPD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124041"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..518)
                     /locus_tag="VFR57_07820"
     CDS             complement(129..518)
                     /locus_tag="VFR57_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259306.1"
                     /translation="MIDRIDHIVLTTRDREGCIRFYRDVLGMELVSFRTPTEERFAFK
                     FGSQKINLHEWGREFAPRAHVAAPGTLDLCFIASKPLERVIENLQKNRIPILEGPVAK
                     TGALGPMRSVYVRDPDLNLVEISVYNK"
     gene            complement(515..1687)
                     /locus_tag="VFR57_07825"
     CDS             complement(515..1687)
                     /locus_tag="VFR57_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0259307.1"
                     /translation="MSGPLAGLRVLELGSLVAGPFCGKTFADFGAEVVKIEPPGEGDP
                     LRKWRRMRNGTSLWWQVQSRGKQSVTCDLRRPEGQEIVRRLARRAHIVVENFRAGALE
                     KWNLGWEVFSHDNPTLVLVRISGYGQTGPYRERPGFAAIAEGMAGFRYVTGFPDRPPV
                     RPNLSIGDTIASLHGVIGALMALHSGKGQVVDVALYEAMFNCMESLIADYDGDGYVRE
                     RTGSTLPGIAPSNLYPCREGYVLIAGNADSLYYRLMTAIGRADLRDDPALARNDGRAA
                     QMERIDEAICAWTRTRTLDEVLNIMEKAQVPAGRIYSAADIAADPHFAARGMIEQVIA
                     GDGEPLKVPGIVPKLSATPGAIRSPAPALGEHTDAVLLAAGYSSEEISGLRKQGIL"
     gene            complement(1684..2121)
                     /locus_tag="VFR57_07830"
     CDS             complement(1684..2121)
                     /locus_tag="VFR57_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEU0259308.1"
                     /translation="MPEEIRTVGRGLAFEDLRLGARFRTHRRTIREADLVAFMTLTGL
                     TEELFAVKEEKQLVPGALVYSFAEGLLLPTMQDTGLAFLNARLDIKGPTYVGDTMHVE
                     CEVTEHRPASKGERGLVRFLNRVITQDNNVVLEYTPLRLLKRR"
     gene            complement(2106..>2384)
                     /locus_tag="VFR57_07835"
     CDS             complement(2106..>2384)
                     /locus_tag="VFR57_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259309.1"
                     /translation="EYVAKLKSQIDNWNAEAAKWEAKAKQAQAGMQADYERQLAQFRT
                     RRDTAMNEVQRLQNASADAWKDMMQGADAAFKSMQAAFEKARSEFQKK"
BASE COUNT          377 a          794 c          765 g          448 t
ORIGIN      
        1 gagctcatgt tgtagccgat cgagccttgc ggggcgctgc caaaacccaa tacctgtgcg
       61 ttcacggccg cgggcagcgc ggcggcgcac aacgcgacaa ccagtctttt catgggggtc
      121 ctcctccgct atttattgta gaccgagatc tccaccaggt tgaggtcggg atcgcgcacg
      181 tacaccgagc gcatcggtcc cagcgcgccg gtcttcgcta ccgggccctc caggatcggg
      241 atgcgattct tctgcagatt ctcgatgacc ctctccaggg gcttggaggc gatgaagcac
      301 aggtcgagcg ttcccggcgc ggcgacatgc gcgcgcggcg caaattccct gccccattcg
      361 tgcaggttga tcttctggct tccgaacttg aaggcgaaac gctcttccgt cggagttcga
      421 aacgacacca gttccatccc cagaacgtcg cggtagaagc ggatgcagcc ttcacggtcg
      481 cgcgtggtga gcacgatgtg gtcaatgcgg tcgatcacaa gattccctgc ttccgcagcc
      541 cggaaatttc ctctgacgaa taccccgccg ccaggagcac tgcgtcggtg tgctcgccga
      601 gcgctggcgc gggcgagcgg attgcgcccg gcgtggcgga gagcttgggc acgatgcccg
      661 gcaccttgag cggctcgcca tcgccggcga tcacctgctc gatcatgccg cgcgccgcga
      721 agtgcggatc ggccgcgatg tcggccgcgc tgtagatgcg tcccgcgggg acctgcgcct
      781 tctccatgat gttcaacacc tcatcaagag tccgggtacg cgtccatgcg cagatcgctt
      841 catcgatgcg ttccatctgc gcggcgcgtc cgtcgttgcg tgcaagcgcc gggtcgtcgc
      901 gcaggtccgc gcgtccgatc gccgtcatca gccgatagta gaggctgtcg gcgttgcccg
      961 cgatcaagac gtagccttct cggcagggat agaggttgga gggcgcaatt cccggcagcg
     1021 tgctgccggt gcgctcgcgc acgtagccgt cgccgtcgta gtcggcgatc aggctctcca
     1081 tgcagttgaa catcgcctcg tagagtgcga cgtcgaccac ctgccctttt ccggaatgca
     1141 acgccatcag ggcgccgatc acgccgtgga gcgaagcgat ggtgtcgccg atcgacaagt
     1201 tcggccgcac cggcggccgg tcggggaagc cggtgacgta gcggaaaccc gccatcccct
     1261 cggcgatcgc ggcgaagccc ggacgctcgc ggtaggggcc cgtctgcccg tagccggaga
     1321 tgcgcacgag cacaagcgtc gggttgtcgt gcgaaaacac ctcccagccg aggttccact
     1381 tctcgagcgc gccggcgcgg aagttctcga ccacgatgtg cgcgcggcgc gcaagacggc
     1441 gcacgatctc ctggccctcg ggccggcgca ggtcgcacgt gaccgattgc ttcccgcgcg
     1501 actgcacctg ccaccagagc gaggtgccgt tgcgcatgcg gcgccacttg cgcagcggat
     1561 cgccctcacc cggcggctcg atcttgacca cttcggcgcc gaagtccgcg aatgtcttgc
     1621 cgcagaaagg cccggcgacg agactgccga gctcgaggac ccgcaagccc gccagcggtc
     1681 cgctcatctc cttttcagca gcctgagcgg ggtgtactcg agcactacgt tgttgtcctg
     1741 cgtgatgact ctgttcaaga accgcacgag gccgcgctcg cctttcgacg cggggcggtg
     1801 ctcggttacc tcgcattcga cgtgcatcgt gtcgccgacg tacgttggcc ccttgatgtc
     1861 gagcctggca ttgaggaaag cgaggccggt gtcctgcatc gtggggagca gcaggccctc
     1921 ggcgaaggaa tacacgagcg cgccgggaac gagttgcttc tcttccttga cggcgaaaag
     1981 ttcctcggta agcccggtga gcgtcatgaa cgcgaccagg tccgcttcgc ggatggtgcg
     2041 gcggtgcgtg cgaaagcgcg cgccgagcct caggtcctcg aacgcaaggc cacgcccgac
     2101 ggttcttatt tcttctggaa ttcgctcctc gccttctcga acgccgcctg catgctcttg
     2161 aacgccgcgt cggcgccctg catcatgtcc ttccaggcgt cggcggaggc gttctgcagc
     2221 cgctgcacct cgttcatcgc cgtatcgcgc cgggtgcgaa actgggcaag ctgacgctcg
     2281 taatcggcct gcatgccggc ttgcgcttgt ttcgccttcg cttcccactt ggcggcctcg
     2341 gcgttccagt tgtcgatctg cgacttgagc ttcgcgacgt actc
//
LOCUS       DASTPD010000237         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_534343, whole genome shotgun sequence.
ACCESSION   DASTPD010000237 DASTPD010000000
VERSION     DASTPD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534343"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1788)
                     /locus_tag="VFR57_07840"
     CDS             complement(106..1788)
                     /locus_tag="VFR57_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0259310.1"
                     /translation="MKPDLKTRHGGKILADALAAQGVKLAFGVPGESYLPVLDGLHDL
                     QDRLRFVICRQEGGASYMAEAYGKLTGEPGVLFVTRGPGATNGAIGVHTGLQDSTPMV
                     VFIGQVPNEFAEREAFQEIDYRRMYGQMAKWVGQIDRVERIPEYVSHAFHTAVAGRPG
                     PVVLALPEDTLFAQAAVADALKHHTVRPAPSNSEIQELKNLLEQAKTPFVLLGGGGWS
                     RDACVRLQKWMEANGLPVGTSFRGQDLFDNRSPNYAGDVGIGINPKLARRVKEADVLL
                     VIGARLGEMTTSAYTLVDVPVPQQTLVHVHAGAEELGRVFRPALAINSGMAQFVASLE
                     GLSFRNPSWKERTAAARKEFLEWTEPRPMPGEMQYGEVIRWLSERLPEDAIIAGGAGN
                     FAGWLHRHFRYKGFRTQLGSTNGSMGYGYPAAVAAKLAQPQRTVFALCGDGDFLMTGQ
                     EIATAVQYGANFVAVVVNNGLYGTIRMHQEREYPGRVYGTELRNPDFAAYARAFGGHG
                     ETVERTADFAAAFERAVASNKPALIEVKIDPDAITPATTLSAIREKSLKSKQ"
     gene            complement(1785..2711)
                     /locus_tag="VFR57_07845"
     CDS             complement(1785..2711)
                     /locus_tag="VFR57_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259311.1"
                     /translation="MPVHPQVALLLEAVRQSPLPTLDKVSAFVARRLYSERCAKVAPQ
                     TPEAHTRLLLTPSGVPIRAYRPAGADKTQALPALVYFHGGGWTIGDLDTHDVLCRQLA
                     NGARCEVFSVEYRLAPESPFPAAVEDCIAATEFVAQSSKSDRIAVGGDSAGGNLAAVV
                     ALHARDTGGPALCFQLLIYPATDQHMRTESVRRNGDGYLLTRVLMELFRANYLPRPAD
                     YADWRASPALAHSHAGLPPALVITAGFDPLLDEGRQYAELLARAGVEVAYRACPDMVH
                     GFIVMGGVLDTANAAVADCCAALRRAFEKVTA"
     gene            complement(2714..3055)
                     /locus_tag="VFR57_07850"
     CDS             complement(2714..3055)
                     /locus_tag="VFR57_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU0259312.1"
                     /translation="MYVEERMYRLKIGAVPEYLKHYQESGMAVQLKHLPHMVGYYFTE
                     VGGLNMVVHMWAYDSLDQRDKCRAAMQADPAWQAYLAKTRPLMETQETRIMKCAPFFV
                     DRLKKMLAAVK"
     gene            3080..3550
                     /locus_tag="VFR57_07855"
     CDS             3080..3550
                     /locus_tag="VFR57_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0259313.1"
                     /translation="MDDINTLRRILKESHVIAVVGLSADWYRPSYFAAKYMQEHGYRV
                     VPVNPKYESILGEKCYRSLRDIAERVDIVDVFRKTQDVMPIAEDAIAIGAKVLWQQLG
                     VRNEAAAAKARAAGLEAVLDRCVKIEHGRLFGGLNWVGVNTKVISAKRPRWLAY"
     gene            3588..4382
                     /locus_tag="VFR57_07860"
     CDS             3588..4382
                     /locus_tag="VFR57_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0259314.1"
                     /translation="MRILEGKFGRLVLSDLAAGEAFSAQADPAIVMQPGEAELVFLNP
                     GESHTAAAATRILALHAATNWLCASFPAVFGAGDGQPFEQAREPITPRIRRLADTLAV
                     EILNDRFVSAERLDFMLQELLLSIVETYLARRRTASPAWRGSRFEDSRIRRAIALLRA
                     RPNKELNMDELASQVGLSRSRFYDLFQLCTGFSPRAYLDMLCVETAISRLTSGHGKIS
                     EVSAELGFSAQSNFTRFFLNQVGVPPSEYRRAATRDASEENPPSGV"
     gene            4418..>4883
                     /locus_tag="VFR57_07865"
     CDS             4418..>4883
                     /locus_tag="VFR57_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0259315.1"
                     /translation="MPDRKFGIETLCLHAGQIPDPATGARAAPIYQTTSFVFDSAEHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERMAAIEGARSALACATGQAAEATALLTLCSGG
                     DHIVSASTLYGGTHTLLHVNLKKLGIETSFVSPDDPENFSKAIRKNTKLLYAE"
BASE COUNT          830 a         1628 c         1580 g          845 t
ORIGIN      
        1 tctacgaggc aaggccggca gtatgccgcg agtatccgga cagcccgcgc tgcgggtact
       61 acgagttcct gaagttcgag cgcgcccacc aggaagaccc gaagttcatt gctttgactt
      121 gagcgatttt tcccggattg cgctgagcgt cgttgccggc gtgatcgcgt cagggtcgat
      181 cttgacctcg atcagcgcag gtttgttgga agcaacggcg cgctcgaagg cggccgcgaa
      241 gtccgcggtg cgctccaccg tctcgccgtg gccgccgaat gcacgcgcgt acgcagcgaa
      301 gtcggggttg cgcagctcgg tgccatacac tcggcccggg tattcgcgct cctggtgcat
      361 ccggatcgtg ccgtagagcc cgttgtttac cacgaccgcg acgaaattag cgccgtactg
      421 caccgccgtg gcgatttcct gtccggtcat caggaagtcg ccgtcgccgc agagcgcgaa
      481 cacggtgcgc tgcggctgtg cgagcttggc ggcaaccgcg gccggatagc cgtaccccat
      541 cgagccgttg gtcgagccga gctgagtgcg gaatcccttg tagcgaaaat gccggtgcag
      601 ccagccagcg aagttgccag cgccgccggc gatgatggcg tcctcgggca agcgctcgga
      661 gagccagcgg atcacctcgc catactgcat ctcgcccggc atcggccgcg gctcggtcca
      721 ctccaggaat tccttgcgcg cggctgcagt tctttccttc caggatggat tcctgaatga
      781 aagaccttcc aacgaagcta caaactgcgc cattccggag ttaatggcga gcgccggccg
      841 gaacacgcgc ccgagttcct ccgcgccggc atgcacgtgg accagcgtct gctgcggcac
      901 cggcacatcg acgagcgtgt acgcgctcgt cgtcatctcg cccaggcgcg cgccaatgac
      961 gagaagcacg tcggcttcct tgacgcgccg cgcgagcttc ggattgatgc cgatgccgac
     1021 gtcgccggca taattcgggc tgcgattgtc gaagagatcc tggccgcgga aagacgtgcc
     1081 gacgggcagc ccgttcgcct ccatccattt ctgcagccgc acacacgcat cgcgcgacca
     1141 gccgccgccg ccgagaagga cgaacggggt ctttgcttgt tctagaagat tcttcaactc
     1201 ctggatttct gaatttgagg gtgccggccg taccgtgtgg tgcttgagcg cgtcggcgac
     1261 cgcggcttgc gcaaacagcg tgtcttcggg cagcgcgagc actaccggcc ccggccggcc
     1321 cgcgaccgcg gtgtggaagg catggctaac gtattccggg atgcgctcga cacggtcgat
     1381 ctggccgacc cacttcgcca tctggccgta catgcgccgg tagtcgattt cctggaacgc
     1441 ttcgcgctcg gcgaattcgt tcggcacctg gccgatgaag accaccatcg gcgtcgaatc
     1501 ctgcaagccg gtgtgcacgc cgattgcgcc gttggtcgcc ccgggtccgc gcgtcacgaa
     1561 gagaaccccg ggctcgccgg tgagcttgcc gtaggcttcc gccatgtagg aagcgccacc
     1621 ctcctggcgg cagatgacga agcgcagccg gtcctgcagg tcgtgcaacc cgtcgagcac
     1681 cggcaggtag ctctcgccgg ggacgccgaa tgcaagcttc acgccctgcg cggcgagcgc
     1741 gtcggcgagg atcttgccgc cgtggcgagt cttcagatcg ggtttcatgc tgtgactttc
     1801 tcgaaagcgc ggcgcagcgc cgcgcagcaa tcagccactg cagcgttcgc ggtgtcgagc
     1861 acgccgccca tcacgatgaa gccgtggacc atgtcggggc acgcgcgata cgcgacctcg
     1921 accccggcgc gcgccagcag ctcggcgtac tggcggcctt cgtcgagcag cggatcgaag
     1981 cccgcggtga tgacgagcgc cggcggcagc ccggcgtggc tgtgggcgag cgcgggcgac
     2041 gcgcgccagt cggcgtagtc ggccggtcgc ggcaggtaat tcgcccggaa aagttccatc
     2101 agcacccggg tgaggaggta gccatcgccg ttgcgtcgca ccgattccgt gcgcatgtgc
     2161 tggtcggtcg ccggatagat aaggagctgg aagcaaagcg ccggtccgcc cgtatcccgt
     2221 gcatgcaacg ccactacggc ggcgagattt ccgcccgcgc tgtcgccgcc aaccgcgatg
     2281 cggtccgatt tggagctctg cgcgacgaac tcggttgcgg cgatgcaatc ctccaccgcg
     2341 gcgggaaacg gcgactccgg cgcgaggcgg tactcgacgg agaaaacttc gcagcgcgcg
     2401 ccgttggcga gctggcggca aagcacgtcg tgcgtgtcga ggtcaccgat ggtccagccg
     2461 ccgccgtgga agtacacgag cgcgggtagc gcttgcgtct tgtcggcgcc ggccgggcgg
     2521 tacgcccgta tcggcacgcc gctcggcgtg agaagcagcc gggtgtgcgc ctcgggcgtc
     2581 tgcggcgcga cttttgcgca ccgctcgctg tagagacggc gcgcaacgaa ggccgaaacc
     2641 ttgtcgagcg tcggaagggg ggactgccgt acggcttcga ggagaagcgc gacctgcgga
     2701 tgaaccggca tcgctacttc acggccgcaa gcattttctt gagccggtcc acgaagaagg
     2761 gcgcgcattt catgatgcgc gtctcctgcg tctccatcag cgggcgggtc ttggcgaggt
     2821 acgcttgcca cgccgggtca gcctgcatcg cggcgcggca tttatcccgc tggtcgaggc
     2881 tgtcgtaggc ccacatgtgc accaccatgt tcagcccgcc gacctcggtg aaatagtagc
     2941 ccaccatgtg cggcaggtgc ttcagctgaa ccgccatgcc cgactcctga taatgcttga
     3001 ggtactcggg cacggcgcca atcttcagtc gatacatccg ctcttcgacg tacatgcttt
     3061 cgattatagt gaacaagcca tggacgacat caacacgctt cggcgcatcc tgaaggaaag
     3121 ccatgtgatc gcggtcgtcg gcctgtcggc cgactggtat cgcccgagct actttgccgc
     3181 gaagtacatg caggagcacg gctaccgggt agtccccgtg aaccccaagt acgagtcgat
     3241 cctgggcgag aagtgctacc gctcgctgcg cgacatcgcc gagcgggtgg acatcgtgga
     3301 tgtcttccgc aagacacagg atgttatgcc gatagccgag gacgccatcg ccatcggcgc
     3361 caaagtgctc tggcagcaac tcggtgtgcg caacgaggcg gcggcggcca aggcccgggc
     3421 cgcaggtctc gaagctgtgc tcgaccgatg cgtaaagatc gagcacgggc ggcttttcgg
     3481 ggggttgaac tgggtcgggg tcaatacgaa ggtcatatcc gccaaacgcc cccgctggct
     3541 ggcttattag acccccggta ggatatcctt cccgcatggg gaggctggtg aggatcctgg
     3601 agggcaagtt cgggcgcctg gtcctgagcg accttgccgc cggcgaagcg ttctccgccc
     3661 aggccgaccc ggccatcgtg atgcagcccg gcgaggcgga gctcgtcttc ctgaacccgg
     3721 gcgaaagcca taccgcagcc gccgcgacac ggattctggc tctgcacgcc gcgaccaatt
     3781 ggctgtgcgc gagcttcccg gcagtcttcg gcgccgggga cggacaaccc ttcgagcagg
     3841 cgcgcgagcc gatcacgccg cgcatacgcc gtctcgccga cacgctggcg gtggaaatcc
     3901 tgaacgatcg cttcgtgtct gccgagcgcc tcgacttcat gctgcaggag ctgctgctct
     3961 ccatcgtcga gacctacctc gcgcgccgac gcaccgcgtc tccggcctgg cgcgggtcgc
     4021 gcttcgagga cagccgcatc cggcgggcga tcgcgctgct gcgcgccagg ccgaacaagg
     4081 agctcaacat ggacgagctc gcgagccagg tggggctctc gcgctcgcgc ttctacgacc
     4141 ttttccagct ctgcaccggt ttctcgccgc gggcctatct cgacatgctg tgcgtcgaga
     4201 ccgcgatctc gcgcctgact tccggccacg gcaagatctc cgaggtctcg gccgaactcg
     4261 gcttctcggc gcagagcaac ttcacgcgtt tcttcctgaa ccaagtgggc gtaccgccct
     4321 ccgagtatcg ccgcgccgcc acgcgggacg cttcagaaga aaatcccccg tcgggggtct
     4381 gaggctcttt cccccggcgc accaggcaaa ccctcccatg cctgatcgca agttcggcat
     4441 cgagacgctt tgcctgcacg ccgggcagat cccggatccg gccaccggcg cgcgcgccgc
     4501 accgatctac cagacgacgt cgttcgtgtt cgactcggcc gagcatgcgg cgagtctttt
     4561 caatttgcag acgttcggca acgtttattc gcgcatctcg aacccgacgg tggcggtatt
     4621 cgaggagcgc atggccgcga tcgagggcgc ccgctcggcg ctcgcctgcg ccaccggtca
     4681 agccgccgag gcaaccgccc tcctgacgct ctgctccggc ggcgaccaca tcgtctcggc
     4741 ttcgacgctc tacggcggca cgcacacgct gttgcacgtg aacctcaaga agctcggcat
     4801 cgagacgagc ttcgtcagcc ccgacgatcc ggaaaacttc agcaaagcca tccggaaaaa
     4861 caccaagctg ctctacgccg aaa
//
LOCUS       DASTPD010000238         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1148829, whole genome shotgun sequence.
ACCESSION   DASTPD010000238 DASTPD010000000
VERSION     DASTPD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148829"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFR57_07870"
     CDS             <1..398
                     /locus_tag="VFR57_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0259316.1"
                     /translation="ETDLAIDYCEDVPPLPLEAASRIAELMREAGLTAKVSSIHVNGW
                     FGDYDKLAMTRRLFAERFAMDLSAANREVVFAGDSPNDSSMFGFFENSVGVANVRRFE
                     SHLSDAPKYVTGAACGAGFAELAEHLLRR"
     gene            514..1737
                     /locus_tag="VFR57_07875"
     CDS             514..1737
                     /locus_tag="VFR57_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0259317.1"
                     /translation="MPGPLEGVRVLDLTRVLAGPWAAQNLADLGAEVIKLERPKKGDD
                     SRAFGPPWLKDAQGCDTSESAYFACANRGKKSLTLDLAHPRGQAIARELAAKCDVLLE
                     NFKFGDLDRYGLGYSQLREVNPGLIYCSVTGFGHTGPWRERPGYDFMIQGMGGLMSVT
                     GERDDRPGGGPQKAGIPIADLITGMYASIAVCAALAHRANTGVGQHLDLALFDSLVAV
                     LANQGGNYLATGKPPGRLGNDHPNIAPYQVFRTADGSLILACGNDKLFRKFCEVAGCL
                     ALADDARFASNGKRVENRVELTRLLNEVFLKRPTKEWIARLEAAGVPCGPINDLSQVF
                     SEPQALARGLRMELPHPSAGGVSLVRSPMRFSQTPVEHGVPPPTLGQHTDEVLRDILG
                     KDVSDIALLRSEGIV"
     gene            complement(1734..>2062)
                     /locus_tag="VFR57_07880"
     CDS             complement(1734..>2062)
                     /locus_tag="VFR57_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259318.1"
                     /translation="KLIPLAITSAKRNPVLPNVPTVQEAGVPGYEVRTWYAVWAIKGT
                     PPAIKERMYKEIVTAMNHPDLKRIWAEQGADPGGMPPAEMEKLVKSEITKWAKVVKDA
                     GARVDN"
BASE COUNT          347 a          665 c          679 g          371 t
ORIGIN      
        1 gggagaccga tctcgcgatc gattactgcg aggacgtgcc gccgctgccg ctcgaggcgg
       61 cgagccgcat cgcggagctg atgcgcgagg cgggccttac cgccaaggtg agctcgatcc
      121 atgtcaacgg ctggttcggc gattacgaca agctcgccat gacgaggcgg cttttcgccg
      181 agcgcttcgc tatggacctc agcgccgcga accgcgaggt ggtgttcgcc ggcgattcgc
      241 ccaacgactc gtcgatgttc ggctttttcg aaaactcggt cggggttgca aacgtccggc
      301 ggttcgaatc gcacctctcc gacgcgccaa agtacgtgac gggcgcggcc tgcggcgcgg
      361 ggttcgcgga gctcgccgag caccttctgc gtcgctgagg tgcgactcgc gtcatagcgc
      421 gcgacgcgag gctgagcgtg ccggcttgcc cgcggtcgtg aacggcgtgc gtagcctgca
      481 caacaacatc acggtcaagt agactgcggc ccgatgccgg ggcccctgga aggagttcgc
      541 gttcttgacc tgacgcgcgt gctggcgggc ccctgggccg cgcagaacct ggccgacctg
      601 ggcgcggagg tcatcaagct cgagcgcccc aagaaaggcg acgattcgcg cgcgttcggt
      661 ccgccctggc tcaaagacgc gcagggttgc gacacctcag aatcggcgta cttcgcctgt
      721 gccaaccgag gcaagaaatc gctgacgctc gacctcgcgc atccgcgcgg ccaggcgatc
      781 gcacgagagc tggcggcgaa atgcgatgta ttgctcgaga atttcaagtt cggcgacctc
      841 gaccgctacg gccttggcta ctcacagctg cgcgaggtga accccggcct tatctactgc
      901 tcggtgactg gcttcgggca tacgggtcct tggcgcgagc ggccggggta cgacttcatg
      961 atccagggaa tgggcgggct gatgagcgtt acgggcgagc gcgacgaccg tcccggggga
     1021 gggccgcaga aggccggcat tccgatcgcc gacctgatta ccgggatgta cgcgagcatc
     1081 gcagtctgcg ccgcgctcgc gcaccgcgcg aacactggcg ttggccagca tctcgatctg
     1141 gcgctgttcg attcactcgt cgcggtgctc gcgaatcagg gggggaatta cctggcgaca
     1201 ggcaagcctc ccgggcgcct gggcaacgac caccccaaca tcgctcccta ccaggttttc
     1261 cgcaccgccg acggaagcct cattctcgcc tgcggtaatg acaaactgtt ccgtaaattc
     1321 tgcgaggtcg ctggctgcct ggcgcttgcc gatgacgctc gcttcgccag caacggcaag
     1381 cgcgtggaga accgggtcga gctgacccgg ctgcttaacg aggttttctt gaaaaggccc
     1441 accaaagaat ggatcgcacg cctcgaagca gcaggggtgc cgtgcgggcc gatcaacgac
     1501 ctttcgcagg tcttcagcga gccgcaggcg ctcgcgcgcg ggctgaggat ggagctgccg
     1561 catccgagcg ccggaggggt atcgctcgtc agaagcccga tgcgcttctc gcaaacacca
     1621 gtcgagcacg gcgtgccgcc cccgacgctg gggcagcaca ccgacgaagt gctaagggat
     1681 attctgggaa aggatgtttc cgatattgct ctgctgcgct cggagggaat tgtctagttg
     1741 tcaacccggg cgccggcgtc tttgacgacc ttcgcccact tcgtgatctc gctcttcacg
     1801 agcttctcca tctccgccgg cggcatgccg ccgggatcgg cgccctgctc ggcccagatc
     1861 cgcttgagat cgggatggtt catcgcggtg acgatctctt tgtacatgcg ctccttgatc
     1921 gccggcggcg tgcctttgat cgcccacacc gcgtaccagg tacgcacctc gtagcccgga
     1981 actccggctt cctgcaccgt cggcacattc ggcaggaccg gattgcgctt cgcgctggta
     2041 atggcgagcg ggatgagctt gc
//
LOCUS       DASTPD010000239        12574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_535721, whole genome shotgun sequence.
ACCESSION   DASTPD010000239 DASTPD010000000
VERSION     DASTPD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535721"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFR57_07885"
     CDS             complement(<1..273)
                     /locus_tag="VFR57_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0259319.1"
                     /translation="MRALVLVVALAVAAVAQARGNVEAGKQKAAQVCAACHGPEGNKP
                     SDPTQPILAGQYYDYLVRAITDYKNGRRNNPIMKGFAAQLSKQDIED"
     gene            complement(270..644)
                     /locus_tag="VFR57_07890"
     CDS             complement(270..644)
                     /locus_tag="VFR57_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0259320.1"
                     /translation="MSGLLIRILIGIAVAFAAHAQPKPPADKAPTVNEKKPPTAMKQG
                     QNFENAVALCTGCHGISGYKTAFPDVYHVPKIGGQQATYLVNALKAYKSGERSHPSMR
                     AIAATLTEEDMKKLADFYAGAK"
     gene            695..1540
                     /locus_tag="VFR57_07895"
     CDS             695..1540
                     /locus_tag="VFR57_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0259321.1"
                     /translation="MRFKGSQSYVSTEDLTLAVNAAITLQRPLLVKGEPGTGKTMLAV
                     EVARSLGVPILEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVQDIHNYIVKGMLWQ
                     AFNAEQPVVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRELVKARHRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMERIVQVHFPTLKKSLLREAMEVFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSKDRKTIIPPLHGALLKNEQDVHLFERLVFMT
                     RAKSS"
     gene            complement(1515..2009)
                     /locus_tag="VFR57_07900"
     CDS             complement(1515..2009)
                     /locus_tag="VFR57_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0259322.1"
                     /translation="MDYLQTLMRYKAWANELVFDAAAKLPEAELRAPRKIVFGSMLRT
                     LNHVYAMDDVWRAHLEGRPHGYTTRNPEACPPFPELRDAQKAMDAWFVRYADSLSRED
                     ETVEFRFIGGGSGAMKRREILLHVANHGTYHRGNVAAMMYQAGTAPPTTDLPVFLRSF
                     SRGS"
     gene            2030..2671
                     /locus_tag="VFR57_07905"
     CDS             2030..2671
                     /locus_tag="VFR57_07905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0259323.1"
                     /translation="MKVTLTFDNGPEPGVTDRVLEVLKSEGIAATFFVLGTKLADPAR
                     RALTVRAHAEGHWIGNHTMTHGTPLGRLQEPGAARREILQAQELLSDLAHPDRLFRPF
                     GGGGALGPHLLNAEALEVLKEERMTCVLWNAIPRDFADPDGWVERALEQCEAHPWALV
                     VLHDLPNGAMGHLPRFIAALRERGAIFEQGFPMECLPITRGKVNADLRPYVTS"
     gene            complement(2815..3084)
                     /locus_tag="VFR57_07910"
     CDS             complement(2815..3084)
                     /locus_tag="VFR57_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0259324.1"
                     /translation="MPKRSRSCGSRTARTRSSTEKACAPIPSTVTSGSPPDWHDLQAH
                     FIYVLKGWITFRFEGVEGEVTVTAGSALSQPAGVPHNVIARLWRP"
     gene            3173..4027
                     /locus_tag="VFR57_07915"
     CDS             3173..4027
                     /locus_tag="VFR57_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259325.1"
                     /translation="MSYHNNPTEPKLKLPAGACDAHFHVFGPRDKFPYAKDAPYVPEV
                     DAPKDVLIDRHMFLGIDRGVVVQSAAHGLDNSATADLLAETKGSYVGVALVPVNADMS
                     LLKKLHEQGFRGARFNYLQHLGGGPAMPELMKLATRLAVLNWHLQLHVESSMIVKMTP
                     ELKLSPVPVVIDHMGRVDASRGLDQPGFRHLLKLMEDKRFWVKVSGLERASRQGSPYA
                     DAVPFARKLVAEFGDRVIWGSDWPHPNLDSIPDDGVLVDLIAEFANEAQRQALLVDNP
                     MKLYKFHA"
     gene            4024..4974
                     /locus_tag="VFR57_07920"
     CDS             4024..4974
                     /locus_tag="VFR57_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259326.1"
                     /translation="MKRLLLGLIFLPLLAFGQAYPNKPVRLIVGFPAGGPADIFGRAL
                     AHGMASELGQPVVVENIAGVGGVLGVDRVAKSAPDGYTLCINSASPLVIAPYSLTKIP
                     YDVKKDFAFVTLVVRVPEVVAVHPSVPAGTLADLISYARANPGKVNFGSAGSGSITHL
                     AGELLKAEAKVDLVHVPYKGAAPAVNDLLGGQVQMGIFDVPVLLAHIRSGKLKALAIT
                     SATRAPSLPEVPTTGELNYPNVNSDNWYGLIMAAATPADIQRRVHSAAVAALKSKAVE
                     EQFSKVGGIASPGSPQEYAKFIASEQTKWSKIVQAIGFKE"
     gene            complement(4964..5380)
                     /locus_tag="VFR57_07925"
     CDS             complement(4964..5380)
                     /locus_tag="VFR57_07925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0259327.1"
                     /translation="MKWIDLSRTMVMPAHCDTYGHMNVRHYAAFFDDAGWHMLAMAGI
                     SLADIRKRGLGTVMVTLTIDFHHEITAGQLALVRGAFTKLGTKSFAYELRLYEADSMT
                     HCATEKAVEVCFDTTERKGIALPDDMRRKLEALATP"
     gene            5409..5552
                     /locus_tag="VFR57_07930"
     CDS             5409..5552
                     /locus_tag="VFR57_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259328.1"
                     /translation="MNRKRNSEKLEPQEQPIEPEMQKRPDEAEHSRDPATEPPKEKRR
                     DDL"
     gene            5567..6349
                     /locus_tag="VFR57_07935"
     CDS             5567..6349
                     /locus_tag="VFR57_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0259329.1"
                     /translation="MFRPNALKRRLAAGRRALGCWTVLGSPPVIELLAYCGFDYLLLD
                     QEHGFGEPSALLHSLQAMAATPNCTSVVRVPSNDAHYLKRVLDAGIEGVMVPNVETAD
                     DARAVVAACRYPPRGRRGSALGSARASDYGISSAKYKSEAANELLIVVQIESPKAVEN
                     IGAIAAVEGIDVLFIGPHDLSGTVGQLGDLKHPEVAKLIARAEEAITKSGKPMGTVPH
                     PGTTWREMFQRGYQMINAGSDVGRLRDAALADIKEFRSVYPE"
     gene            6333..7007
                     /locus_tag="VFR57_07940"
     CDS             6333..7007
                     /locus_tag="VFR57_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0259330.1"
                     /translation="MFTLNSLPPPLEAQLVAKLLKAEPATIGHFRNTGFMDPAIRAMQ
                     SDVRIAGPAVTVRAPGMDGTIVGYALGQVRRGDVLVIDRAGDHRHAAFGGLVAYACKV
                     AEIAGVIIDGMATDIGEVRKYGVPLWCRGLSAVTTKRIGLAGEFCVPVSCGGVSVHPG
                     DIIIADECGIVVLPRPEAEGAAERAIGMQDAEVNSRKRLDSGEKLPDISGATKVLQEA
                     MAKQKS"
     gene            complement(6991..8178)
                     /locus_tag="VFR57_07945"
     CDS             complement(6991..8178)
                     /locus_tag="VFR57_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0259331.1"
                     /translation="MPSDARLAQITDRLTPDLVALRKQLHQHPELAFEEHETAKAVTA
                     FLGKHKIAARTGIGKTGVIAMVEGAKPGPTIGIRADMDALPIHEQTGLPFASKVAGKM
                     HACGHDVHTVIALGAAVALAEMRDSLRGKVKFIFQPAEETLSGARAMIADGALDDPKM
                     DLILGYHNWPAVPAGKVGYNAEAVMSSADAFDVMLKGRDGHGAHPHLAVDALAAGAYF
                     VSQLQTIVSREVKPLSPAVVTVGEFHAGTARNVIAGTAHLKGIVRTMEAGLSEKIEAA
                     MRRMLEGMKLGMRIDYELKWDRVAPALRNNKDVLAKLLSSARNELGADNVLELPEPSM
                     GSEDFAWFAEKVPAAHLRIGSKIDGLDTAIHRADYDCNELAIPIGVRVVARAALDLAS
                     SSA"
     gene            8194..9060
                     /locus_tag="VFR57_07950"
     CDS             8194..9060
                     /locus_tag="VFR57_07950"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0259332.1"
                     /translation="MVVRPLQGEFAAEVIGLRLPVDDATFRDVELAWLRHPILVFRDL
                     QMTPQQHIAFTRRFGPPHIMEPLQYNLPGYPEIFVVSNVEEGGKALGMKRAGWGWHTD
                     GEDKAIPNAGSMLYALELPLEGGDTLYADGYAAYEALPADVKDRIRGRRACFSRIKLH
                     HVHYPHLPALTEEDKRKRPDVWHPLERRHPKSGRTALYIGRWACAIEGMPEDEGARLV
                     RWLQEFSVRPEFVYRHRWRLHDAVLWDNRCAQHCATPFDDAKYRRHMLRTTLEGDAPM
                     MATSPARSLASP"
     gene            complement(9026..10003)
                     /locus_tag="VFR57_07955"
     CDS             complement(9026..10003)
                     /locus_tag="VFR57_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259333.1"
                     /translation="MSEPALRLAGIEKCYGATVAVAGVDLEVREGEFLTLLGPSGCGK
                     TTTLGLIAGFFAPSAGEIFLKGRPVAGLPSFKRDIGVVFQDYALFPHMTAGQNVAFGL
                     KMRKTAPDEIGQRVKEALDLVQLRGLAERRPLELSGGQRQRVALARALVIRPAVLLLD
                     EPLSNLDLKLREEMRVEIAGIQRTLGITTVFVTHDQGEALVMSDRIAVMNAGRIEQIG
                     RPAEIYEKPASRFVAEFIGRMNFFQANGKTVAIRPERARLSREQPSSGFARQGTVRNV
                     LYLGATLEYHLDLQGERALVEAPNDGAGPRFQVGDPVWFTATRESALEM"
     gene            complement(9996..10799)
                     /locus_tag="VFR57_07960"
     CDS             complement(9996..10799)
                     /locus_tag="VFR57_07960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259334.1"
                     /translation="MSPWSALRAVVIFVYVFMLGPILITAAVSFNESNRSQFPPRGFS
                     LQWWGRALSAEWVDPLWFSLKLGTITALISTLLALPLAFVLARHRFPGRDAMVALTLG
                     PLMLPALVTGVGLLQLFQYAGLREHIGFTALLVGHVVICLPFAVRTVAISVHTLPPNV
                     ELAAASLGASRWRTLLHVVFPLIKSGIVAGAVFAFVHSFTDVNLSLFLVRPGEVPITV
                     KILGFLEFGFAPTLAAVSVITLLLPLVLVVIVERVTGLGDFIYGQRDRV"
     gene            complement(10796..11644)
                     /locus_tag="VFR57_07965"
     CDS             complement(10796..11644)
                     /locus_tag="VFR57_07965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259335.1"
                     /translation="MARDHGLRIWLLVAAPMALLLVFFVIPNALLLSTSFLRSEAQQL
                     TGELTLENYTFLFTRPIYIGAFVRTFAVGASVGLLCVLLGYPLAYFLVRTKSRWKGVL
                     IALSLAPLLASVVVRTYGWYIILNRFGVANDVLLGLGLTSERIAFMPSTGAIVIGLAH
                     ALLPYTVLTIMGSLNGINPNLEQAAMSLGATRTRTFFHVVLPLSLPGIAGGFILAFSI
                     AISAYATPAILGGPATQVMATAIYGFMTQLLDWSIGAALAVILILSSLLLLYAASRIG
                     AKQAAI"
     gene            complement(11623..>12574)
                     /locus_tag="VFR57_07970"
     CDS             complement(11623..>12574)
                     /locus_tag="VFR57_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0259336.1"
                     /translation="YQELVERVVIRPFEKKFGVKVIHDATGTASQDYAKIRAARGAPG
                     FDVAGLLTPPEVILGVKEGLLEKLTEREVPNLKHLWDKTWQVIPGGSGAPHTLQYAAL
                     VYNREKIERPRSWADYWEPQKRYGDKIKGRVINYNPANLLSVYALIHAAQLGGGGVDN
                     MEPAWQRLKAQKPHVGVVVTGSAEAVPHFENGQVWISPYWSGRSGYYIDRGLPFDMVI
                     PQEGVIGLYDVACVPVGAKNKKLAYEFLNWRLDKEIQRQWALAYFTSPTRSDVSDLPP
                     KFAATQIVTKEQMAKTQFPDSDVIGARRKDWTLKWQEIMA"
BASE COUNT         2246 a         4121 c         4080 g         2127 t
ORIGIN      
        1 gtcctcgatg tcctgcttgg aaagctgcgc ggcgaagccc ttcatgatgg gattgttgcg
       61 ccggccgttc ttatagtcgg tgatggcgcg caccaggtaa tcgtagtact gccccgcgag
      121 gatcggctgt gtcggatcgg acggcttgtt gccctcgggg ccgtggcagg cggcgcagac
      181 ctgcgcggcc ttctgctttc cggcttccac gttgccgcgc gcttgcgcaa cggcggcgac
      241 tgccagcgcg acaaccagga cgagcgccct catttcgccc ccgcgtagaa gtcggccagt
      301 ttcttcatgt cttcctcggt gagcgtcgcg gcgatggcac gcatggacgg atgcgaccgc
      361 tcgccggatt tgtaggcctt gagcgcgttg accaagtaag tcgcctgctg gccgccgatc
      421 ttcggtacgt gatagacgtc ggggaaagcg gtcttgtacc cggaaatgcc gtggcagccg
      481 gtgcacagcg ccaccgcgtt ctcgaagttc tgtccctgct tcatcgccgt cggcggcttc
      541 ttttcgttga ccgtcggcgc cttgtcggcg ggcggtttcg gctgggcgtg ggcggcgaac
      601 gccaccgcga tgccgattaa gattcttata aggaggccgg acatggcagc ggcgcattct
      661 agccgaaaac actccccggg ctagaatccg cggcatgcgt ttcaaaggct cgcagtccta
      721 cgtctccacc gaagacctga ccctcgcggt gaacgccgcg attaccctcc aacgcccgct
      781 cctcgtcaag ggcgagccgg gaaccggaaa gacgatgctc gcggtggagg tggcacgctc
      841 gctcggcgtc cccatcctcg agtggcacat caagtcgacc accaaggcgc agcagggcct
      901 ctatgaatac gacgcagtga gccggctgcg cgactcacag cttggcgacc cgcgcgtgca
      961 ggacatccat aactacatcg tgaagggcat gctctggcag gcgttcaacg ccgaacagcc
     1021 ggtggtgctc ctgatcgacg agatcgacaa ggccgatatc gagtttccga acgacctgct
     1081 gcgcgagctc gaccgcatgg agttctacgt ctacgagacg cgcgagctcg tcaaggcgag
     1141 gcaccgcccg atcgtcttca tcacctcgaa taacgagaag gagctgccgg acgcgttcct
     1201 gcgccgctgc tttttccact acatccgttt cccggacaag gagacgatgg agagaatcgt
     1261 ccaggtgcac tttccgaccc tcaagaagtc actcctgcgc gaggcgatgg aggtcttctt
     1321 cgaggtgcgc gaggttcccg ggctgaagaa gaaaccttcg acttccgagc ttctcgactg
     1381 gctgaagctg ctcctagccg aggacatccc gccggaagcg ctgcgttcca aagaccgcaa
     1441 gaccatcatc ccgccgctgc acggggcgct cctgaagaac gagcaggacg ttcacctgtt
     1501 cgagcgccta gtgttcatga cccgcgcgaa aagctcctaa gaaataccgg caggtcggtg
     1561 gtcggcggcg ccgtgccggc ctgatacatc atcgccgcga cattgccgcg gtggtacgta
     1621 ccatggttcg ccacgtggag gaggatctcc cggcgcttca tcgctccgct gccgccgccg
     1681 ataaaccgga actccaccgt ttcgtcttcg cgggacaggg aatcggcata tcgcacgaac
     1741 cacgcatcca tcgccttctg cgcgtcgcgt agctccggga acggcggaca ggcttcgggg
     1801 ttgcgggtgg tatagccgtg cggccgtcct tccagatggg cgcgccaaac gtcatccatt
     1861 gcatagacgt ggttcagggt acgcagcatg ctgccgaaca cgatcttgcg tggcgcgcgg
     1921 agctcggctt ccggcagctt cgccgccgcg tcgaatacga gctcgttcgc ccacgccttg
     1981 tagcgcatca gcgtttgcag atagtccatc gagctagagt accgccaaag tgaaagtcac
     2041 cctcaccttc gacaacgggc ctgaacccgg cgtcaccgac cgcgtcctcg aagtcctgaa
     2101 gagcgaaggc atagccgcca ccttcttcgt gctcggcacg aagctcgccg atccggcgcg
     2161 tcgcgcactc accgtccggg cgcatgccga aggccactgg atcggcaacc acaccatgac
     2221 gcacggtacg ccgctcggcc gtttgcagga accgggcgcc gcgcggcgcg agatccttca
     2281 ggcgcaagag cttctcagcg acctcgcgca tcccgatcgc ctgttccgac ccttcggggg
     2341 cggcggcgcc ctcggccctc acctcctcaa cgccgaagcg ctcgaggtgc tgaaggaaga
     2401 gcgcatgacc tgcgtgctct ggaacgccat cccgcgcgat tttgccgacc ccgacggctg
     2461 ggtcgagcgc gccctcgagc agtgcgaagc gcacccctgg gcgctcgtcg tcctgcacga
     2521 cctgcccaac ggcgcgatgg ggcacctgcc ccgcttcatt gcggcgctgc gcgagcgcgg
     2581 cgcgatcttc gagcagggct ttcctatgga gtgccttcca attacgcgcg gcaaggtcaa
     2641 cgcagatctg aggccttacg ttacgtcgta gtgccgccgc caggcgcttt ccaagcgccg
     2701 catcggtttc attccctaga gcgtgagatt gacggcgccg ccgtcgatca gcaggttctg
     2761 cccgacgatg aagcccgcgt gcgcgctcgt gcgaacgcgc ctggcgctgc gtgctcaagg
     2821 tcgccagagg cgcgcgatca cgttgtgcgg cacgcccgca ggctgcgaca gcgcggagcc
     2881 ggcggtcacc gtgacctcgc cctccacgcc ctcgaagcgg aacgtgatcc agcctttgag
     2941 gacgtagatg aagtgcgcct gcaggtcgtg ccagtcgggc ggcgagcccg aggtcacggt
     3001 actcggaatc ggcgcgcagg ccttctcggt ggaacttcgc gtcctcgccg tgcgcgagcc
     3061 gcagctccga gagcgcttcg gcaagcggtt tcgggccagc gtacgcgggc tttgccatgc
     3121 caggaactcc ttacaccggg cgggagcttg cccataaaat cggctcgcga tcatgagcta
     3181 ccacaacaat ccgacagaac cgaagctcaa gctccccgcc ggggcgtgcg acgcgcattt
     3241 ccacgtcttc ggtccgcgcg acaagttccc gtacgcgaag gacgcgccct acgtaccgga
     3301 ggtcgatgcg cccaaggacg tgctcatcga tcggcatatg tttctcggca tcgaccgcgg
     3361 tgtggtggtg cagtccgccg cccatggcct cgacaattcg gcgacggccg atctcctcgc
     3421 cgagacaaag ggaagctacg tcggtgtggc gctcgtcccg gtgaatgcgg acatgtcgct
     3481 tctcaagaag ctgcacgagc agggcttccg cggcgcgcgc tttaattatc tgcagcatct
     3541 cggtgggggg cctgcgatgc ccgagttgat gaagctcgcc accaggctcg cggtgctcaa
     3601 ctggcacctg cagctccacg tcgagagctc gatgatcgtg aagatgaccc ccgagctcaa
     3661 gctttcgccg gtgccggtgg tgatcgatca catgggacgc gtcgacgcct cgcgcggcct
     3721 cgaccagccg ggcttcaggc atcttctcaa gctcatggag gacaagcgct tctgggtgaa
     3781 agtgagcggc ctcgagcgcg cctcgcgcca gggctcaccc tatgccgatg cggtgccctt
     3841 cgcgcgtaag ctcgtcgccg agttcggcga tcgcgtgatc tggggcagcg actggccgca
     3901 tccgaacctc gattcgattc ccgacgacgg cgtgctcgtc gacctgatcg ccgagttcgc
     3961 caacgaggca cagcgccagg cactccttgt cgacaacccg atgaagctct ataaattcca
     4021 cgcatgaagc gactgctgtt aggcttgatt tttcttcccc tcctggcctt cgggcaggcc
     4081 tatccgaaca aaccagtccg gctcatcgtc ggctttccgg ccggtgggcc tgcggacatc
     4141 ttcggccgcg cgctcgcgca cggcatggca agcgagctgg gtcagccggt cgtggtggag
     4201 aacatcgcgg gcgtcggcgg cgtgctcggt gtcgatcgcg tggcgaaatc cgctcccgac
     4261 ggctacacgc tctgcatcaa cagcgcgtca ccgctcgtga tcgcgcccta ctcgctcacc
     4321 aagattccct acgacgtaaa gaaggacttt gccttcgtca cgctcgtggt gcgcgtgccg
     4381 gaggtcgtgg cggtccatcc gtccgttccg gcgggcacgc tggcggatct catctcgtac
     4441 gcaagagcga atcctggcaa agtgaacttc ggctcggccg gtagcggcag cattacccac
     4501 ctcgcgggcg agcttctcaa ggccgaagcg aaagtcgatc tcgtccacgt cccctataag
     4561 ggcgccgcgc cggccgtgaa cgacctgctt ggcggccagg tgcagatggg catcttcgac
     4621 gtgccggtgc tcctcgccca catccgcagc ggcaagctga aagcgctcgc catcacgagc
     4681 gcgacgcgcg cgccctcgtt acccgaagtg cccacgactg gcgagctcaa ctacccgaac
     4741 gtcaactcgg acaactggta cgggctcatc atggccgccg cgacgccagc cgacatccag
     4801 cggcgcgttc acagcgcggc ggtcgccgcg ctcaaatcaa aagcggtgga ggagcagttc
     4861 tcgaaggtgg gcggcatcgc ttcgcccggc tcgccgcagg aatacgccaa attcattgcg
     4921 tccgagcaga cgaagtggtc gaagatcgtg caggcgatcg gcttcaagga gtagcgagcg
     4981 cctcgagctt tcgccgcatg tcgtcgggga gcgcgatgcc cttgcgctcc gtggtgtcaa
     5041 agcacacttc gaccgccttc tccgtcgcac aatgcgtcat cgaatccgcc tcgtagaggc
     5101 gcaactcgta agcgaagctc ttcgtcccta atttggtgaa ggcgccgcgc acgagcgcga
     5161 gctgtccggc ggtgatctcg tgatggaagt cgatggtgag cgtcaccatc accgtgccga
     5221 gtccgcgctt gcggatgtcg gcaagcgaga ttcccgccat cgcgagcatg tgccagccgg
     5281 cgtcgtcaaa gaacgcggcg tagtggcgca cgttcatatg gccgtaggtg tcgcagtgcg
     5341 ctggcatcac catcgtgcgc gagaggtcga tccatttcat atgggcatga tcattgcggc
     5401 agccgggcat gaaccgcaag cgaaattccg aaaagctcga gccgcaggaa cagcccatcg
     5461 agcccgagat gcagaaaagg cccgatgagg ccgagcacag tcgcgacccg gcgaccgagc
     5521 cgcccaaaga gaagcggcgc gacgatctct aggtaaactt cgggcggtgt tccgcccgaa
     5581 cgcactgaag cggcgcctcg ccgcgggccg gcgcgcgctc ggctgctgga ccgtgctggg
     5641 gagcccgccg gtcatcgagc tcctcgccta ctgcgggttc gactatctgt tgctcgacca
     5701 ggagcacggc ttcggcgagc cttcggcgct tctgcattcc ctgcaagcga tggccgccac
     5761 gccgaactgc acctcggtcg tgcgcgtgcc atccaatgat gcgcattatc tgaagcgcgt
     5821 gctcgacgcg ggcatcgaag gggtgatggt gccgaacgtg gaaacggcag acgacgcgcg
     5881 cgccgtcgtg gccgcgtgcc gctacccgcc ccggggccgc cgcggttccg cgctcggatc
     5941 ggcgcgcgct tccgactacg ggatttcttc agcgaaatac aagagcgagg cagcgaacga
     6001 gttgctgatc gtggtgcaga ttgagtcgcc gaaagcggtc gagaacatcg gcgcgatcgc
     6061 ggcggtcgaa ggcatcgacg tcctcttcat cggtccgcac gatctctcgg gtaccgtcgg
     6121 gcagctcggc gacctcaagc acccggaagt cgcgaaactc atcgcgcgcg ccgaggaagc
     6181 gatcacgaaa agcggcaagc cgatgggcac cgtgccgcat cccggcacca cgtggcgcga
     6241 gatgttccag cgcggctacc agatgatcaa cgcggggagc gacgtcgggc gtctgcgcga
     6301 cgcggcgctc gccgatatca aggagttccg tagtgtttac cctgaatagc cttccaccac
     6361 ccctggaggc gcagctcgtc gcgaagttgc tgaaagccga gcccgcgacc atcggccact
     6421 tccgaaacac cggcttcatg gacccggcga tccgcgccat gcagtccgac gtgcgcatcg
     6481 ccggcccggc ggtcacggta cgcgcgccgg gcatggacgg cacgatcgtc ggctacgcgc
     6541 tcggacaagt gcggcgcggc gacgtgctcg tcatcgaccg cgcgggcgac caccgccacg
     6601 ccgcgttcgg cgggctggtc gcctatgcgt gcaaggtggc ggaaatcgcc ggcgtaatca
     6661 tcgacggcat ggcgaccgac atcggcgagg tgcgcaagta cggcgttccg ctttggtgcc
     6721 gcgggctctc ggccgtcacg acgaaacgca tcggcctcgc gggcgagttc tgcgtgccgg
     6781 tcagttgcgg cggcgtctcc gtgcatccgg gcgacatcat catcgcggat gaatgcggca
     6841 tcgtcgtgct gcctcgtccg gaggcggagg gcgcggccga gcgggccatc ggcatgcagg
     6901 acgccgaagt caattcgcgc aagcgcctcg actcgggcga aaagctgccc gatatctcag
     6961 gcgcgacgaa agtgcttcaa gaagcgatgg ctaagcagaa gagctagcaa gatcgagagc
     7021 cgcgcgcgcg acgacacgca cgccgatcgg aatggcgagc tcgttgcagt cgtagtcggc
     7081 gcggtggatc gccgtgtcga ggccgtcgat cttcgagccg atgcgcaggt gcgccgccgg
     7141 gactttctcg gcgaaccagg cgaagtcctc gctccccatc gaaggctcgg gcagctcgag
     7201 cacgttgtcc gctccgagct cgttgcgcgc gctggaaaga agctttgcga gcacgtcctt
     7261 gttgttcctg agcgccggcg cgactcggtc ccacttgagc tcgtaatcga tgcgcatgcc
     7321 gagcttcatg ccctcgagca tgcggcgcat cgcggcttcg atcttctccg aaagcccggc
     7381 ctccatggtg cgcacgatgc ccttcaagtg cgcggtgccg gcgatgacgt tgcgcgcggt
     7441 gccggcgtgg aactcgccca cggtcacgac cgccggcgat agcggcttca cctcgcggct
     7501 tacgatcgtc tgaagttgag aaacgaagta cgcgcccgcg gcgagcgcat cgaccgcaag
     7561 gtgcggatgc gcgccgtgac catcgcggcc cttgagcatc acatcgaacg cgtcggccga
     7621 cgacatcacc gcctcggcgt tgtagccgac cttgcccgcc ggcacggccg gccagttgtg
     7681 atagccgagg atcaaatcca tcttcggatc gtcgagcgca ccgtcggcga tcatcgcgcg
     7741 cgcgccggag agcgtttcct cggccggctg gaagatgaac ttcaccttgc cgcgcagcga
     7801 atcgcgcatt tcggcgagcg cgaccgccgc gccgagcgcg atcacggtgt gcacatcgtg
     7861 cccgcacgcg tgcatctttc ccgccacttt ggaggcgaat ggcagtccgg tctgctcgtg
     7921 gatcggcagc gcgtccatat cggcgcggat gccgatcgtc ggccccggct tcgcgccctc
     7981 caccatcgcg atcacgccgg tcttgccgat gccggtgcgc gcggcgatct tgtgcttgcc
     8041 gagaaacgcc gtgaccgctt tcgccgtctc gtgctcctcg aacgcgagct cgggatgctg
     8101 atgcagctgc ttgcgcagcg cgacgaggtc gggagtgaga cggtccgtga tctgggcgag
     8161 gcgcgcgtcg gagggcatgg cagaattatc cgaatggtgg ttcgtccgct gcaaggggag
     8221 ttcgctgccg aagtgatcgg cctgcgcctg ccggtcgacg atgccacctt ccgcgacgtc
     8281 gagctggcct ggctgcgcca tccgattctg gtgttccgcg atctgcagat gacgccgcag
     8341 cagcacatcg cctttactcg gcgcttcggc ccgccgcaca tcatggagcc gctgcagtac
     8401 aacctgcccg gctacccgga gatattcgtc gtctccaacg tggaggaagg cggcaaggcg
     8461 ctcggcatga agcgcgccgg ctggggttgg cacaccgacg gcgaggacaa ggcgatcccg
     8521 aatgccggct cgatgctcta cgcgctcgag ctaccgcttg aaggcggcga cacgctctac
     8581 gccgacgggt atgcggcgta cgaggcgctt cctgctgacg taaaggacag aatccggggc
     8641 cgtcgcgcct gcttcagccg gatcaagctc catcacgtcc actacccgca cctcccggcg
     8701 ctcaccgaag aggacaagcg caagcgaccc gacgtatggc acccgctcga gcgccggcac
     8761 cccaagtccg gccgcaccgc cctctacatc ggccgctggg cgtgcgccat cgaaggcatg
     8821 cccgaggacg agggcgcacg gctcgtgcgt tggctgcagg agttctccgt gcgccctgaa
     8881 ttcgtctacc gccaccgctg gcgcctgcac gacgcggtgc tctgggacaa ccgctgtgcc
     8941 cagcactgcg ccacgccgtt cgacgacgcg aagtatcgcc gtcacatgct gcgcaccacc
     9001 ctcgagggcg acgcgccgat gatggctaca tctccagcgc gctctctcgc gtcgccgtaa
     9061 accacaccgg gtcgccgacc tgaaagcgcg gcccggctcc gtcgttcggc gcttcgacga
     9121 gcgcgcgctc gccttgtaag tcgaggtggt actcgagcgt cgccccgaga tataggacgt
     9181 tgcgaaccgt tccctggcgc gcgaagccgc tcgagggctg ctcgcgcgag agccgcgcgc
     9241 gttcaggccg gatggcgacc gtcttgccgt ttgcctgaaa gaaattcatg cggccgatga
     9301 actcggcgac gaatcggctc gccggcttct cgtagatctc cgccggccgg ccgatctgct
     9361 cgatacggcc cgcgttcatc acggcgatgc gatccgacat gacgagcgcc tcgccctggt
     9421 cgtgcgtgac gaacaccgtg gtgatgccga gcgtgcgctg gatcccggcg atctccacgc
     9481 gcatctcttc gcgtagcttg agatcgaggt tggagagcgg ctcgtcgagc aagagcaccg
     9541 cgggccggat gacgagcgcg cgtgcaagcg cgacgcgctg gcgctgcccg cccgagagct
     9601 cgagcgggcg gcgctcggcg aggccgcgca gctggactag gtcgagcgcc tctttgacac
     9661 gttggccaat ctcatccggc gcggtctttc tcatcttcaa gccgaaagcg acgttctgcc
     9721 ccgcggtcat gtgcggaaag agcgcgtaat cctggaacac gacgccgatg tcgcgcttga
     9781 aggacggaag gccggcgacc ggccgccctt tgaggaagat ctcacccgcg ctcggcgcga
     9841 aaaagccggc gatgagcccg agcgtggttg tcttgccgca gcccgaagga ccgagcagcg
     9901 tcaggaactc gccttcgcgc acttccaagt cgacgcccgc cacggcgacg gtggccccgt
     9961 agcacttctc gatgcccgca agccgcaggg ccggctcaga cacgatcgcg ctgaccgtag
    10021 atgaaatcgc cgaggcccgt cacacgctcc acaatcacga caagaaccag cggcaagagg
    10081 agcgtgatca ccgagaccgc ggcaagcgta ggcgcaaagc cgaattcgag aaaaccgagg
    10141 atcttcacgg tgatcggcac ctcgccgggc cgtacgagga aaagagagag attgacgtcg
    10201 gtaaacgagt gcacgaaggc gaacaccgcg cccgcgacga tgccactctt gatgagggga
    10261 aatacgacgt gcaacagcgt gcgccagcgc gatgcgccga gactggcggc cgcgagctct
    10321 acgttcggcg ggagcgtatg cacgctgatc gccaccgtgc gcaccgcgaa cggcaggcag
    10381 atcaccacgt gcccaacgag aagcgccgta aatccgatgt gttcgcgaag acccgcgtat
    10441 tggaagagct ggagcagccc gacgccggtc acgagcgccg gaagcatgag cgggccgagc
    10501 gtgagcgcca ccatggcgtc gcgtccgggg aatcgatgac gcgcaagcac gaaggcgagc
    10561 ggcagcgcca gcagcgtgct aatgagcgcg gtgatcgtgc ctagcttgag actgaaccac
    10621 agggggtcga cccactccgc cgacagcgcc ctgccccacc attgcagcga aaacccgcgc
    10681 ggcgggaact gcgagcggtt cgattcgttg aaggacaccg ctgcggtgat gaggatcggc
    10741 ccaagcatga agacgtacac gaagatcacc accgcgcgca gcgcactcca ggggctcata
    10801 tcgcggcctg cttcgctccg atccgagagg cggcatacaa aagcagaagc gaagaaagga
    10861 tgagaatcac cgcgagcgcg gcgccgatcg accagtcgag gagctgggtc atgaagccgt
    10921 aaattgcggt ggccattact tgcgttgcag ggccgccaag gatcgccggc gtggcgtagg
    10981 cgctgatcgc aatcgagaac gcaaggataa agccgccggc gatgccggga aggctgagcg
    11041 gaagcacgac gtgaaagaag gtgcgcgtgc gggtcgcgcc gaggctcatc gcggcctgct
    11101 cgaggttggg attgatgccg ttcagcgacc ccatgatggt gagtacggtg tacggcaaaa
    11161 gcgcatgcgc gaggccgatg acgatggcgc cggtgctcgg catgaaggcg atgcgctcgc
    11221 tcgtcaggcc aaggccgagc agtacgtcgt tcgccacgcc gaagcggttg aggatgatgt
    11281 accagccgta ggtccgcact acgacgctcg cgaggagcgg cgcgagtgag agcgcgatga
    11341 gcacgccctt ccatcggctt ttcgtgcgca ccaggaagta cgcgagcgga taaccgagca
    11401 gcacgcacag cagtcccacc gaggcgccga cggcaaaggt gcgcacgaaa gcgccgatgt
    11461 agatcggccg cgtgaaaagg aacgtgtagt tctcgagcgt gagctcgccg gtgagctgct
    11521 gcgcctctga tcttaggaag ctcgtcgaga gaagcagcgc gttcggaatg acgaaaaata
    11581 cgagcagcag cgccatcggc gctgccacga gaagccagat tctcaggcca tgatctcttg
    11641 ccacttgagc gtccagtcct tgcggcgggc gccgatcacg tccgaatccg ggaactgggt
    11701 cttggccatc tgctccttgg tcacgatctg cgtggcggcg aacttgggcg gcaggtcgct
    11761 gacgtcgctg cgtgtcgggc tggtgaaata agcgagcgcc cattggcgct ggatttcttt
    11821 gtcgaggcgc cagttgagga actcataggc gagcttcttg tttttcgcgc cgaccggcac
    11881 gcaggccacg tcgtaaagtc cgatcacgcc ttcctgggga atcaccatgt cgaacggcag
    11941 gccgcggtcg atgtagtagc cggagcggcc gctccagtaa ggcgagatcc agacctggcc
    12001 gttctcgaaa tgcggcacgg cttcggccga gccggtgacc accacgccga cgtgcggctt
    12061 ctgcgccttc aagcgctgcc aggcgggctc catgttgtcg acacccccgc cgcccagctg
    12121 cgcggcgtga atcagcgcgt agaccgagag caggttcgcc gggttgtagt tgatgacgcg
    12181 gcccttgatc ttgtcgccgt agcgcttttg cggttcccag taatcggccc acgagcgcgg
    12241 gcgctcgatc ttctcgcggt tatagacgag cgcagcgtac tgcagcgtgt gcggggcgcc
    12301 cgagccaccg ggaatcacct gccaagtctt gtcccacagg tgcttgaggt tcggcacttc
    12361 gcgctcggtc agcttctcca gcaacccttc ctttacgcca aggatcacct cgggcggcgt
    12421 gaggagaccc gccacgtcga atcccggtgc gccgcgcgcg gcgcggatct tggcgtagtc
    12481 ctgcgacgcg gtgccggtcg catcgtggat cactttcacc ccgaacttct tttcgaacgg
    12541 ccggatcacg acgcgctcga caagctcctg gtac
//
LOCUS       DASTPD010000240         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1352540, whole genome shotgun sequence.
ACCESSION   DASTPD010000240 DASTPD010000000
VERSION     DASTPD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352540"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFR57_07975"
     CDS             complement(<1..349)
                     /locus_tag="VFR57_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU0259337.1"
                     /translation="MRLLQMSAMEFAQEMEQALATNPFLEENPDAAASTPRDASVEEV
                     VLPQQGSSGAAPVERDQEDWGGVSDAQPTLQEHLREQLRMSRVSDRDRALAEMVVDSL
                     DDDGYFKATFDELA"
     gene            531..>2610
                     /gene="ligD"
                     /locus_tag="VFR57_07980"
     CDS             531..>2610
                     /gene="ligD"
                     /locus_tag="VFR57_07980"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU0259338.1"
                     /translation="MQVAAIMPDRLRHYREMRDFDVTPEPRGKKARPRRKRLREAFLR
                     YYIQRHDATRLHYDLRLELEGVLKSWAVPKGPSLDPADKRLAMQTEDHPLEYGEFEGV
                     IPEKQYGAGDVLLWDKGVWTPEDTDPAAALAKGRLHFRLDGEKLRGSWILTRTSRGDE
                     RAAWLLIKRHDEEARPGIDITLEQPESVKRASKAAGRASRAALPQFVTPQLATLVTRP
                     PKTGDWVYEVKHDGYRMLGRFSENQVRLFTRSGRDWTDKLPHLARELQRLSLDGAWLD
                     GEIVVLRPDGRSSFQALQNAFEGGADAKIVYYVFDAPFLDGRDLRQLPLRERKARLRK
                     ALKASGAVRFSEDLAGATHEILEHASKLGLEGLIGKQADSVYVAGRTRSWIKLKCRLT
                     QDFVIAGYTKPGGSRHGFGALVLGVYERPGRLVYAGKVGTGFDEDLLRRLTRKFTDFK
                     KPDSALANPPRERDIQWLRPALVAEVEFTERTDDGVIRQGSFLGLREDIPAKSVGMEK
                     PQAAPESEPSRTIKITHSDRLIWPELGIRKIDLVRYFEEIGEWFVPHVANRPLTLVRC
                     PDGIGEECFYQRHLNMGASPGDVLTFKRARSSKGYYIYVNSLPGVLRVVQNGAVEFHT
                     WGATVPAAKHPDRITIDLDPDPDPELRWRQVVEGATLTRALIEGLKLRCFLKTTGGKG
                     LHVVFPIKRRH"
BASE COUNT          480 a          836 c          849 g          445 t
ORIGIN      
        1 cggcgagctc gtcgaaagtc gccttgaagt agccatcatc gtccaaactg tcgaccacca
       61 tctctgcgag cgcgcggtcg cggtcgctta cccgcgacat gcgcagctgc tcgcgcaggt
      121 gctcttggag cgtcggttgc gcgtccgaaa cgccgcccca gtcctcctgg tcgcgctcga
      181 ccggggcggc gcccgacgaa ccttgctgag gcaggacgac ctcttcgacc gaggcgtccc
      241 gcggcgtaga cgccgctgcg tccggatttt cctcaaggaa cggattggtg gcgagcgcct
      301 gctccatctc ctgcgcgaac tccatcgcag acatctggag caagcgcaac gcttgttgaa
      361 cctgcggcgt cagcgtaagg tgctgccgca gtcttaatgc tattgctgga cccatggttt
      421 tccgtagatc tgcaaaggcc atgccagcgc gctttgcgcc tagggggcca cggcgtcccc
      481 gctgtaataa ttgcgcgcgc cgcgcagcaa ttaccgtacg ggctcgcggt ttgcaggtcg
      541 ctgccatcat gcccgatcgc ctgcggcact accgcgagat gcgcgatttc gacgtcacgc
      601 ccgagccccg cggcaagaaa gctcgcccgc gcagaaagcg gcttcgcgag gcctttcttc
      661 gttactacat ccagcgccac gatgcgaccc ggctgcacta tgacttgcgc cttgagctcg
      721 aaggcgtgct caaaagctgg gccgtgccca aaggcccgag ccttgatcct gccgacaagc
      781 gcctcgccat gcagaccgag gaccacccgc tcgagtacgg tgaattcgaa ggcgtgatcc
      841 cggaaaagca gtacggcgcg ggcgacgtgc tgctgtggga caagggcgtg tggaccccgg
      901 aagacacgga tccggcggcg gcgctcgcca aggggcggct ccacttccgc ctcgacgggg
      961 agaagcttag gggctcttgg atcctgacgc gcacgagccg cggggacgag cgagccgcat
     1021 ggctgctcat caagaggcac gatgaagaag cacggccggg catcgacatc actcttgagc
     1081 aacccgaaag cgtgaagcgc gcgtcgaaag ccgccgggcg cgcaagccgc gccgcacttc
     1141 cgcagttcgt cactccgcag ctcgcgaccc tcgtcacgcg gccgccgaaa accggcgatt
     1201 gggtttacga ggtaaagcac gacggctatc gaatgctcgg cagattttct gagaaccagg
     1261 tccggttgtt cactcgcagc ggaagagatt ggaccgacaa actcccgcac ctcgcgcgcg
     1321 agctgcaaag gctcagcctc gatggcgcgt ggctcgacgg cgagatcgtg gtgctgcgcc
     1381 ccgacgggcg ctcgagcttc caggcgctgc agaacgcgtt cgaaggcggc gcggatgcca
     1441 agatcgtcta ctacgtgttc gatgctccgt ttctggacgg gcgcgacttg cgtcagttgc
     1501 cgttacggga acgaaaggct cgtctgagga aagctctgaa ggcaagcggc gccgtccggt
     1561 ttagcgaaga cctcgcagga gccacgcacg agatccttga gcacgcctcc aagctgggcc
     1621 tcgaggggct gatcggcaag caggccgatt cggtttacgt tgccgggcgc acgaggagct
     1681 ggatcaagct caagtgccgc cttacccagg acttcgtgat tgccggctac accaagcccg
     1741 gcggctcgcg ccatggcttc ggcgcgctgg tgctcggcgt ctacgagcgg cctggcaggc
     1801 tggtttatgc gggcaaggtg gggaccggct tcgacgagga tttgttgagg agattgaccc
     1861 ggaaattcac tgatttcaag aaacccgatt ctgcgcttgc gaatccgccg cgcgagcggg
     1921 acatccagtg gctgcggccc gcgcttgtcg ccgaagtgga attcaccgag cgtaccgacg
     1981 acggcgtaat ccggcagggc tcgttcctgg ggctgcgcga ggacattccg gcgaagtcgg
     2041 tgggaatgga gaagccgcaa gctgcgccgg aaagcgagcc ttcgaggacg atcaagatca
     2101 cgcattccga ccggctgatc tggccggagc tcgggatccg caagatcgac ctcgtgcgct
     2161 attttgagga gatcggcgaa tggttcgtgc cgcacgtggc gaaccgcccg ctgaccctcg
     2221 tgcgctgtcc cgacggcatc ggagaggaat gcttctacca gcgccacctc aacatggggg
     2281 cgagcccggg cgatgtgctc accttcaagc gggcgcgctc gagcaagggc tactacatct
     2341 atgtgaacag cctgccgggc gtgctccgcg tggttcagaa cggtgcggtc gaattccaca
     2401 cctggggcgc cacggtgcct gccgccaagc acccggaccg catcaccatc gacctcgacc
     2461 ccgatcccga tcccgagctg cggtggcgcc aggtggtgga aggcgcgact ctgacccgag
     2521 cgctcatcga gggcctcaag ctgagatgct ttctaaagac gaccggtggc aagggcctcc
     2581 acgtcgtctt tccgatcaag cggcgccact
//
LOCUS       DASTPD010000241        13556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1353943, whole genome shotgun sequence.
ACCESSION   DASTPD010000241 DASTPD010000000
VERSION     DASTPD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353943"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFR57_07985"
     CDS             complement(<1..436)
                     /locus_tag="VFR57_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0259339.1"
                     /translation="MKNIVLAAVLAALAAPAAAQTAGAPPSPSGKFGFVHTERILRDS
                     APAMRAQKKIEAEFQKRDRELAGIAADLKRMQAELDKEAMTMTESQRRTKEREFGELN
                     RDFQRKQREFREDLNQRRNEELAQVVEQANRVIRQIAEQEKFD"
     gene            complement(436..2721)
                     /gene="bamA"
                     /locus_tag="VFR57_07990"
     CDS             complement(436..2721)
                     /gene="bamA"
                     /locus_tag="VFR57_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0259340.1"
                     /translation="MLRNTGVIAAVVAFCLAVASASAAAQEFQPFVVKDIRVEGLQRT
                     EPGTVFSYLPVKVGETMNTEKARAALRALYATGFFQDVRLETENDVLVVFVQERPAIA
                     QIDFSGMKEFEPDNVRKVLRELGMAEGRIFDRSVLDAAEQELKRQYLSRGMYAAQVQT
                     TVTPLERNRVGINIAVTEGPVAKIRGINIVGAQAFPERELLGLFVLRTPGWLTWYTQH
                     DRYSRERLSADLETLRSFYLNRGYLDFSLESTQVQITPDRRDIHITVNIVEGETYTVS
                     DVSLAGQLLLPREELEMLIQLKPGDVFSREKLAASTKAISERLGNEGYAFANANAIPN
                     VDKAKRTVAFSIVVDPGRRVYVRRVDVAGNSKTRDEVIRREMRQLEGAYYDASKIQLS
                     RRRIDRTNFFSEVTVETLPVESSPDQVDVVYTVKEKATGSLLLGVGFSSVERIALSAS
                     ITQANAFGTGKYLSANINSGSVNQVYSLSYMDPYYTVDGVSRGFDVYRRVTDASDLAV
                     GPYKTDSLGGGIKFGYPVAEQVSIDVGANLESVDLEIFSNSPLSYLNFVREFGSQYTY
                     VSGTAGWTRDARDSLILTTAGSLQRASIELAGGDLQYYRLGYQHQWYTPLSRTLTLHL
                     RADIGYGGGYNDKPLPFFKNYFAGGPGTVRGYRPFSLGPLDELGNSTGGNRKLTGTAE
                     ILFPMPGAEQDRSLRLAGFVDGGQVYVDKIELSELRYSTGIALFWSSPMGPLRLSWAY
                     PLNAKDADRKQQLQFTFGTGF"
     gene            complement(2722..4086)
                     /gene="rseP"
                     /locus_tag="VFR57_07995"
     CDS             complement(2722..4086)
                     /gene="rseP"
                     /locus_tag="VFR57_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU0259341.1"
                     /translation="MTLLHTIVAFIIALGLLIVVHEYGHYIVARWCGVKVLRFSVGFG
                     RPLFSWRRGRDRTEWVIAAIPFGGYVKMLDEREGPVDAAEVHRAFNRQSVWRRFSIVV
                     AGPLANFLFAIMAYAGLFMYGLPEARPVLGAPPAGSTAALAGLRSGDTVHAVDGEAIT
                     TWQELRWRVLQSALQRAPLRMEVVNEKGHLRDVLLDLRSFPADDVEADALERIGLRLY
                     RPPLDPVIGQVVRSGAAERAGLAPGDRVVLADGKPVDSWEALVAAVQAKPELPLNLTI
                     ERDGAQQTVEVVPASVAAGERRIGRIGAAPQLPPSHAERMLIRVQHGIGESLWKALLK
                     TGDIAVFSLKMLGKMLLGEVSWKHLSGPVTIADFAGQSAAMGWVSYLTFLALISISLG
                     VLNLLPIPLLDGGHLMYYAIEIVKGKPVSERAMELGQRVGLALLLVMMAFAFYNDLNR
                     LLTG"
     gene            complement(4097..5248)
                     /locus_tag="VFR57_08000"
     CDS             complement(4097..5248)
                     /locus_tag="VFR57_08000"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0259342.1"
                     /translation="MNLAIFGATGSIGASTLDVAARHPDRYRVFALTANGSTAQLAEL
                     CARHRPRFAVLSGVREEGSLRRTFAGFGTELLFGAQALERLARLPEVDTVMAGIVGAA
                     GLASTLAAVCAGKRVLLANKEALVMAGPLVLRSARQSGAQLVPVDSEHNAVFQCFSRK
                     EFVRKIVLTASGGPFRAAAPESLAGVTPEQACAHPNWTMGRKISVDSATMMNKGLEVI
                     EARWLFDLPPERIEVLIHPQSIVHSLVEYADGSVIAQLSTPDMRVPIAHALAYPERVE
                     SGAQSLDLVSIRNLSFEKPDAARFPCLALAYSALRAGGAAPAVLNAANEIAVEAFLAG
                     RLAFTGIAGVIADTLAALPAGQAHELAQVMEADARARELARMRILARAA"
     gene            complement(5245..6027)
                     /locus_tag="VFR57_08005"
     CDS             complement(5245..6027)
                     /locus_tag="VFR57_08005"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0259343.1"
                     /translation="MRDLLRRTGTAAVLLAALVAALFLLPRPGILALMALVAGAGALE
                     WARLCAINAPLYAGAVVLALFVAFSLDVSQAVFLLATAFWLFAAPAWLRLRVEPEHRP
                     ALAAAGFVVLVPAALAMVVLEPGEALLVLAVAWIADTGAYFAGTRWGRRKLAPAISPG
                     KTWEGAAGGLIGAAAYAMILSFILAGAQATRMAAFVGAAALLVTVGIVGDLFESAAKR
                     QAGVKDSGALLPGHGGVLDRIDSATAILPVAALVVPFLKSTP"
     gene            complement(6020..6799)
                     /gene="uppS"
                     /locus_tag="VFR57_08010"
     CDS             complement(6020..6799)
                     /gene="uppS"
                     /locus_tag="VFR57_08010"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU0259344.1"
                     /translation="MTQSPGEPSSTREIPNAAELPRHVAIIMDGNGRWAQRRRLPRIA
                     GHRRGADAVRATVRACAERGIEYLTLFAFSSENWRRPAEEVALLMKLFKSALENEVEQ
                     LHGNGVRVKVVGDTQRFDPQIRALIERADKLTAQNRRLTLTIAANYGGRWDILQAMTR
                     LLRESSPSLDRVDEELVTPYLAMSYAPEPDLFIRTGGEQRISNFLLWQLAYTELYFTD
                     LLWPDFDAAALDEAIASYRRRERRFGRTGEQVQQSKRADVA"
     gene            complement(6796..7356)
                     /gene="frr"
                     /locus_tag="VFR57_08015"
     CDS             complement(6796..7356)
                     /gene="frr"
                     /locus_tag="VFR57_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0259345.1"
                     /translation="MVIAELKKTTEQKMDKSVQAFKADLAKVRTGRAHTGLLDHITVD
                     YYGTQTPLNQVAKVVLIDARTIGVTPFEKKMAQAIEKAIRDSDLGLNPASSGETVRVP
                     MPPLTEERRKELIKVVKHEAENARVAVRNLRRDAIHQLKEALKKHEVSENDERRALEE
                     MQKMTDRHVAAIDRLLQDKEKELLAV"
     gene            complement(7373..8086)
                     /gene="pyrH"
                     /locus_tag="VFR57_08020"
     CDS             complement(7373..8086)
                     /gene="pyrH"
                     /locus_tag="VFR57_08020"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0259346.1"
                     /translation="MTPKYKRVLLKLSGEALMGEDPFGINRQTIETIVGEVAQVSRLG
                     VELGVVIGGGNIFRGIAPGAAGMDRATADYMGMLATVMNALALSDAMRQAGIASRVQS
                     ALNIEQVVEPYIRGKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGSEMGAEIVLKG
                     TKVDGVYTADPKRDPRAERYARISFDEAIARNLRVMDATALTLCRDQKLPIAVFSIFK
                     PGALRRVVLGEDEGTLVHV"
     gene            complement(8083..8964)
                     /gene="tsf"
                     /locus_tag="VFR57_08025"
     CDS             complement(8083..8964)
                     /gene="tsf"
                     /locus_tag="VFR57_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0259347.1"
                     /translation="MAEISAGLVKELRERTGLPMMECKKALAETGGDLKKAEELLRIK
                     GSAKATKAADRIAAEGVIGAWLSADGKVGSLVELNSETDFVAKNDDFIGFAKSLAEIV
                     GMRNPSSVGELSLLDLLGSKVEARRQALIQKIGENMTIRRFTRMEAKGKLALYIHAGS
                     RIGVLVDYEGPEDVGKDVAMHIAFAKPAYLSKVQVPQEIVLDERRVQEARAKESGKPA
                     DIIAKIVEGAVNKALNEKTLLGQPFVRDDKRTVEKMLVAKKAKVHGYAFFVVGEGIEK
                     KSTDFAAEVMAQAGKAH"
     gene            complement(9034..9783)
                     /gene="rpsB"
                     /locus_tag="VFR57_08030"
     CDS             complement(9034..9783)
                     /gene="rpsB"
                     /locus_tag="VFR57_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0259348.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMAPYIFGHRNKIHIINLEK
                     TLALYQEALKFARQLTANRGTILFVGTKRQARDIVREEAIRCQMPFVDHRWLGGMLTN
                     FKTVKQSIKRLKEMEAMQQDGSLERISKKEALGLTRELAKLQRSLGGIKDLGALPDAL
                     FVIDVGYQKGAVAEANKLGVPVIGVVDTNHNPEGIGYVIPGNDDSSRAIRLYARGIAD
                     AVLEGRTQSLEEVVAASRDEFVEVEEDEASQ"
     gene            9893..10681
                     /gene="map"
                     /locus_tag="VFR57_08035"
     CDS             9893..10681
                     /gene="map"
                     /locus_tag="VFR57_08035"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0259349.1"
                     /translation="MAVVLKSPEELGRMRVAGRLASEVLDYITPHVRAGVSTGKLNDL
                     CHHYMVDVQGTVPAPLNYAPPGYRPFPKSICTSVNHQVCHGVPGERVLKPGDILNIDI
                     TVIKDGFHGDCSRMFYVGEPSIQAKRLVEVTFECMWLGIRQVRPGARLGDVGAAIQSH
                     AERHGFSVVREFCGHGIGRRFHEEPQVLHYGKPGVGVALEPGMTFTIEPMINAGKPGI
                     RELADGWTIVTKDHSLSAQWEHTVAVTGSGHEVLTKSNGTPAPP"
     gene            10678..13218
                     /locus_tag="VFR57_08040"
     CDS             10678..13218
                     /locus_tag="VFR57_08040"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEU0259350.1"
                     /translation="MKFDLRAEVAAERQALREAYLRRPNPRALLAKHTQLVDRTAKAL
                     WGEAAVATEAALVATGGYGRGELFPSSDIDLLVLLGTDPSEAEREALERLIGRLWDIG
                     LEIGHSVRTVEGCAEAAAGDVTIRTTLLESRFLVGSRALFRRLEQALVFDPVAFFKAK
                     KLEQEQRHAKHQDSPYSLEPNLKEAPGGLRDLQIIQWIARASGMGRRWTDLVAHKLIQ
                     RDEARQLSRLERHLHDLRIRLHYLAGRREDRLVFDLQTALAKQLGYEDTPARLASEAM
                     MQRYYRTAQGVTQLNTILLQNLEARLAPRPDTAPRPLNERFEVRSELLHIRQEGTFAR
                     TPSAILECFLLMMQHPELRGMTARTLRALWRARSLVNGKFREDSIARLLFLHILQQPR
                     GIVHEFRRMNQYGILGRYLPEFGRIVGQMQHDLFHVYTVDQHILMVVRNLRRFTMPEF
                     AHEFPLCTELMAGFERRWLLYVAALYHDIAKGRGGDHSELGTRDARAFARNHGLAQQD
                     RELVEFLVQRHLAMSHVAQKQDVYDPEVVRAFADLVRTERRLVALYLFTVADVRGTSP
                     KVWNAWKGKLLEDLFRATRRVLTGEPLARDAALAEKQGEAARLLRLYALSDAVKEKLW
                     ASVDTAYFLRHDAQEIAWHTRNLHYRVDTDKPVVKARLAPFGEGLQVMVYTRDREALF
                     ARICGYFERAGFSIAEAKVHTTRNGYALDTFVVMGEGRDAHYQDRIAMIETELAAEVQ
                     SGAPLGPPRGGRLSRRVRHFPISPRVDVRPDERGAYHVLSIVAADRPGLLYGVARILA
                     RNRINLQTARINTLGDRAEDVFLISGESLGDSKTVLQLEQDLLEELQI"
     gene            complement(13215..>13556)
                     /locus_tag="VFR57_08045"
     CDS             complement(13215..>13556)
                     /locus_tag="VFR57_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259351.1"
                     /translation="FLHISKQEQKKRLLERESDPKTAWKLNANDWKEREYWDEYTEAY
                     EDAISKTAAPHAPWTVVPADAKWYRNLVIAESVVEALRGRRADWKKTLDAMARKRRAE
                     LAAYRAPQARG"
BASE COUNT         2249 a         4537 c         4399 g         2371 t
ORIGIN      
        1 tgtcgaactt ctcctgctcc gcgatctgcc ggattacccg gttcgcctgc tcgacgacct
       61 gcgcgagctc ctcgtttcgg cgctggttca ggtcctcgcg gaactcgcgc tgctttctct
      121 ggaagtcgcg gttgagctcg ccgaactcgc gctccttggt gcggcgctgg ctctcggtca
      181 tggtcatggc ttccttgtcc agctccgcct gcatgcgctt caagtcggca gcgatcccgg
      241 cgagctcgcg gtcgcgcttc tggaactcgg cttcgatctt cttctgcgcc cgcatcgcgg
      301 gggccgagtc gcgcaggatg cgctcggtat gcacgaagcc gaacttgccg ctaggggacg
      361 gcggagcgcc agcagtctgc gcagccgccg gtgccgccag cgccgcgagg actgccgcga
      421 ggacgatgtt cttcattaga accctgttcc gaacgtgaat tgtaactgct gcttccggtc
      481 cgcgtctttc gcgttcagcg ggtaggccca agacaggcgc agcggtccca tcggcgagga
      541 ccagaacagc gcgattcccg tcgagtagcg tagctcgctg agttcgattt tgtcgacgta
      601 cacttgcccg ccgtcgacga aaccggcgag gcgcagcgat cggtcctgct cggcgcccgg
      661 catcgggaaa aggatttccg ccgtgccggt aagtttgcgg ttgccgccgg tcgaattgcc
      721 gagctcgtcg agcggaccca gtgagaacgg gcggtagccg cgcaccgtgc cggggccgcc
      781 agcgaaatag ttcttgaaga acggcaacgg cttgtcgttg tagccgccgc cgtagccgat
      841 atcggcacgc aggtgcaggg tcagcgtgcg cgacagcggg gtataccact gatgctggta
      901 gccaagccgg tagtactgca ggtcgcctcc tgcgagctcg atcgaagcgc gctggaggct
      961 gcccgcggtg gtgaggatta ggctgtcgcg cgcatcgcgc gtccagcctg cagtgcccga
     1021 tacgtaagtg tactggctgc cgaactcgcg cacgaagttg aggtacgaca gcgggctgtt
     1081 tgagaagatc tccaggtcca ccgattccag gttggcaccg acgtcgatcg atacctgctc
     1141 ggcgaccggg tagccgaact tgatgccgcc gccgagcgag tcggtcttgt acgggccgac
     1201 cgcgaggtcc gaggcgtccg tgacgcgtcg atagacgtcg aagccgcggc tcacaccgtc
     1261 gaccgtgtag tacgggtcca tgtaggaaag cgagtacacc tggttcacgc tgccgctgtt
     1321 gatgttggcc gacaggtact ttccggtgcc aaacgcgttc gcctgcgtga tggaggccga
     1381 cagtgcgatg cgctccacgc tggagaagcc cacaccgaga agcagcgacc cggtcgcctt
     1441 ctccttcacc gtatagacca cgtccacctg gtcggggctg ctctcgaccg ggagggtctc
     1501 gacggtaacc tcgctgaaga aattggttcg atcgatgcgc cgtcgcgata gctggatttt
     1561 ggacgcgtcg taataagcgc cctccagctg tcgcatttcg cggcggatca cttcgtcgcg
     1621 cgtcttgctg ttgccggcga cgtcgacgcg ccgcacgtag acccggcgcc ctgggtccac
     1681 cacgatgctg aaggcgacgg tgcgcttcgc cttgtcgacg ttcggaatcg cgttcgcgtt
     1741 cgcgaaggcg taaccctcgt tgcctaggcg ctcggagatc gccttggtgc tcgcggcgag
     1801 cttctcgcgc gagaatacat cgccgggctt gagctggatc agcatctcca gctcttcgcg
     1861 cggcaggagc agttggccgg cgaggctcac gtcggatacg gtgtaggttt caccttccac
     1921 gatgttgacg gtgatatgga tgtcgcgtcg gtcgggcgtg atctggactt gcgtcgattc
     1981 caggctgaag tcgaggtagc cgcgattcag gtaaaaggag cgcagcgtct cgagatcggc
     2041 gctcagtcgc tcgcgcgaat agcggtcgtg ctgggtgtac caggtgagcc agccgggggt
     2101 gcgcaggacg aacagcccga gcagctcgcg ctcgggaaag gcctgcgcac cgacgatgtt
     2161 gatgccgcgg atcttggcca ccgggccctc ggtcaccgcg atattgatgc cgacgcggtt
     2221 gcgctcgagc ggcgtcaccg tggtctgcac ttgcgcggca tacatgccgc gcgacaggta
     2281 ctgacgcttc agctcctgct ccgcggcgtc gagcaccgag cggtcgaaga tgcggccctc
     2341 cgccatgccg agctcgcgca gcaccttgcg cacgttatcg ggctcgaact ccttcatgcc
     2401 ggaaaagtcg atctgagcga tcgccgggcg ctcctgcacg aacaccacca ggacgtcgtt
     2461 ttccgtctca agccgcacgt cctggaagaa accggtggcg taaagcgcgc gcagcgcggc
     2521 acgcgctttt tcggtattca tggtctcgcc gaccttgacc ggcaggtagc taaagacggt
     2581 gcccggctcc gtacgctgca gcccttcgac gcggatatcc ttgaccacaa agggctggaa
     2641 ctcctgggct gccgcgctgg cagacgccac ggccaggcaa aaggcgacca ccgcggcgat
     2701 cacgccggtg ttgcgcagca tctagccggt caggaggcga ttcaggtcgt tgtaaaaggc
     2761 aaaagccatc atgacaagca gcagcgcaag gccgacgcgc tgtccgagct ccatggcgcg
     2821 ctcggatacc ggcttcccct tcacgatttc aatcgcgtag tacatgagat gcccgccgtc
     2881 gagcagcgga atcggcagca ggttgagcac ccccaggctg atgctgatca aggcgaggaa
     2941 cgttaggtac gagacccagc ccatggcggc cgactggccg gcgaagtcgg caatcgtgac
     3001 cgggccggaa agatgcttcc acgacacctc gccgagcagc atcttgccga gcatcttcag
     3061 gctgaacacc gcgatgtcgc cggtcttcag cagcgccttc cataagctct cgccgatccc
     3121 gtgctgcacg cgaatcagca tccgctcggc gtgcgacggc ggcaactgcg gagccgcgcc
     3181 gatgcggccg atgcgccgct cgcccgctgc gaccgaggcc ggcacgacct cgacggtttg
     3241 ctgcgcgcca tcgcgctcga tcgtcaggtt cagcggcagc tcgggcttgg cttgcaccgc
     3301 cgctaccagc gcttcccagg agtccacggg tttcccgtcc gcgaggacga cccggtcgcc
     3361 gggcgcgagg ccggcacgct ccgccgctcc gctgcgcacc acctggccaa tcacgggatc
     3421 gagtggtgga cggtacagcc gcaacccgat gcgctcgagc gcgtcggcct ccacatcgtc
     3481 cgccggaaac gagcgcaaat cgagcaatac gtcgcgcaga tggccttttt cgttgacgac
     3541 ttccatgcgc aacggggccc gctgcagcgc agattgcaga acccgccagc gcagctcctg
     3601 ccacgtggtg atcgcttcgc cgtcgaccgc gtgcacggtg tcgcctgagc gcagcccggc
     3661 caaggccgcg gtcgagccgg cgggcggcgc accaagcacg ggacgcgctt ccggcagccc
     3721 gtacatgaaa agcccggcat aggccatgat ggcgaacagg aaattggcga gcggcccggc
     3781 gaccacgatg ctaaagcggc gccacacgct ctggcggttg aacgcgcggt gcacctcggc
     3841 cgcgtcgacc ggtccttcgc gctcgtcgag catcttgacg tagccgccga acgggatggc
     3901 ggcgatgacc cattcggtgc gatcgcggcc gcggcgccag ctgaacagcg ggcggccgaa
     3961 gccgaccgag aagcgcagca ccttcacgcc gcaccagcgc gcaacgatgt agtggccgta
     4021 ctcgtgcacg acgatcagca gtccgagcgc gatgatgaac gcgacgatgg tgtggagcag
     4081 tgtcatcggg ccgaagtcag gcagcgcgcg ccagtattcg catgcgcgcg agctctctcg
     4141 cgcgtgcgtc ggcttccatt acttgggcga gctcgtgcgc ctgtcctgcg gggagcgctg
     4201 cgagcgtatc ggcgatgacc ccggcgatgc cggtgaacgc aagccggccg gcaaggaagg
     4261 cttccaccgc gatctcgttg gcggcgttga gcaccgcggg tgctgcgccg ccggcgcgca
     4321 gggcggaata ggcgagcgca aggcacggaa agcgcgcggc atccggcttc tcaaatgaca
     4381 gatttcgtat cgaaacgaga tcgagggact gagctcccga ctccacgcgc tcgggatagg
     4441 caagcgcgtg cgcgatcggc acacgcatgt cgggagtgga aagctgcgcg atcaccgagc
     4501 cgtcggcgta ctcgaccagc gaatgcacga tgctctgcgg atggatgagc acctcgatgc
     4561 gctcgggcgg aaggtcgaac aaccagcgtg cctcgatgac ctcgaggccc ttgttcatca
     4621 tggttgcaga gtccaccgag atcttgcggc ccatggtcca gttcgggtgc gcgcatgcct
     4681 gctccggcgt cacgcccgcc aggctctccg gcgcggctgc gcggaacggg ccgcccgaag
     4741 cggtaagcac gatctttcgg acgaactcct tcctcgagaa gcactgaaat acggcgttat
     4801 gctcgctgtc caccggcact agctgcgctc cggactggcg ggccgagcgc agaacgagcg
     4861 gcccggccat aaccagcgcc tctttgttcg cgagcagcac gcgcttgcct gcgcacactg
     4921 cggcgagagt cgatgcaagc cccgcggcgc ccacgatgcc agccatgacc gtatcgactt
     4981 cgggaagccg cgccagcctc tcgagcgcct gggcgccgaa cagaagctcg gtgccgaatc
     5041 cggcaaacgt gcgccgaagg ctgccctctt cccggactcc cgacagcacg gcaaagcgcg
     5101 gccggtgacg cgcgcacagc tcggcgagct gcgcggtcga gccgttagca gtgagcgcga
     5161 atacccggta gcgatcaggg tggcgtgcgg ctacgtcgag cgtgctcgcg ccgatggatc
     5221 cggtagcgcc gaagatggcg agattcacgg cgtgctcttc aggaagggca cgaccagggc
     5281 cgccacggga aggatcgccg tcgcgctgtc gatgcgatcg agcacgccgc cgtgcccggg
     5341 cagcagcgct ccgctgtcct tgaccccggc ctgccgcttg gcagcagact cgaacaggtc
     5401 gccgacgatg cctaccgtta cgagcagcgc cgccgcgccg acaaaagccg ccatccgggt
     5461 cgcctgagcc cctgcgagaa tgaacgaaag aatcatagcg taggcggcag cgccgatgag
     5521 cccgccggct gcgccttccc aggtcttgcc tggactgatc gccggggcga gcttgcgccg
     5581 gccccaccgc gtgccggcga aatacgcgcc cgtgtcggca atccatgcga ccgcaaggac
     5641 gagtagcgct tcgccgggct cgagcaccac catcgcgagc gcggccggca cgagcacgac
     5701 aaacccggca gccgccagcg cgggccggtg ctccggctct acccgcagcc gcagccatgc
     5761 cggcgcggcg aaaagccaga acgcggtggc gagcaggaac accgcctgcg atacgtcgag
     5821 cgagaaagcg acgaacaagg cgagcaccac cgcgccggcg taaagcggcg cgttgattgc
     5881 gcacaggcgc gcccactcga gcgccccggc gccggccacg agcgccataa gcgcgaggat
     5941 gccggggcgc gggagtagga aaagcgccgc gaccagcgcg gcgagcagca cggcggcggt
     6001 gccggtccgc ctcaggaggt cacgcaacgt cggcgcgctt cgattgctgc acctgctcgc
     6061 ccgtgcgccc gaagcgccgc tcgcgcctgc ggtaggaagc gatggcctcg tcgagcgcag
     6121 cggcgtcgaa gtccggccac agcaggtcgg tgaaatacag ctcggtgtat gcgagctgcc
     6181 acagcaggaa gttgctgatg cgctgctctc ctccggtgcg gatgaagaga tccggctcgg
     6241 gcgcatagct catcgcgagg tacggcgtga cgagctcttc gtccacgcga tcaagcgaag
     6301 gcgagctctc tctcagcagc cgggtcatgg cctgcaaaat gtcccacctg ccgccgtagt
     6361 tggccgcgat ggtgagggtg aggcggcggt tttgcgcggt cagcttgtcg gcgcgctcga
     6421 tcagcgcccg gatctgcgga tcaaagcgct gcgtgtcccc gacgactttc acgcgcacgc
     6481 cgttgccgtg gagctgctcg acttcgttct ccagcgcgct cttgaaaagc ttcataagga
     6541 gcgccacctc ctcggccggg cggcgccagt tctccgagct gaaggcgaac agcgtcaggt
     6601 actcgatgcc gcgctcggcg caggcgcgca ccgtggcgcg caccgcgtcg gcgccgcgcc
     6661 gatggccggc gatgcgcggc agccgccggc gctgcgccca gcggccgttg ccgtccatga
     6721 tgatggcgac gtgacgcggc agctcggctg cgttcgggat ttcacgggtg gagctcggct
     6781 ccccgggcga ttgggtcata ccgcgagcag ctccttctcc ttgtcctgca gcagccggtc
     6841 gatcgcggca acatgccggt cggtcatctt ctgcatctcc tcgagcgcgc gccgctcgtc
     6901 gttctccgag acctcgtgct tcttgagcgc ctccttgagc tggtggatgg cgtcgcggcg
     6961 caggttgcgg accgccacgc gcgcgttctc ggcctcgtgc ttgaccacct tgatcagttc
     7021 cttgcgccgc tcctcggtga ggggtggcat cggcacgcgc acggtttcgc cgctggatgc
     7081 ggggttcaag ccgaggtcgg agtcgcggat cgctttctcg atcgcctgcg ccatcttctt
     7141 ctcgaacggg gtgacgccga tggtgcgcgc gtcgatcagc accaccttgg cgacctggtt
     7201 cagcggcgtc tgcgtgccgt agtagtcgac cgtgatgtga tcgagcagcc ccgtgtgcgc
     7261 gcgcccggtg cgcaccttcg ccaggtcggc tttgaacgcc tggacgctct tgtccatctt
     7321 ctgctccgtc gtcttcttga gttctgcaat caccatagcg acctccccgc catcatacgt
     7381 gaaccagcgt gccctcgtcc tcgccgagga ccacgcggcg cagtgcgccg ggcttgaaga
     7441 tgctgaatac cgcgatcggc agcttctggt cgcggcacag cgtgagcgct gtggcatcca
     7501 tcacgcgcag attgcgcgcg atcgcctcgt cgaagcttat tcttgcgtag cgctcggcgc
     7561 ggggatcgcg cttcggatcg gcggtataca cgccgtcgac tttcgttccc ttgagcacga
     7621 tctcggcgcc catctcgctg ccacgcagcg ccgcagcggt atccgtggtg aagaacgggt
     7681 tcccggtccc agccgcgaag atcaccacct tgccctcctc aaggtagcgg atcgccttgc
     7741 ccctaatata gggctccacc acctgctcga tgttgagcgc cgactgcaca cggctcgcga
     7801 tcccggcctg gcgcatcgcg tccgagagcg cgagcgcgtt catcacggtg gcgagcatcc
     7861 ccatgtagtc ggcggtcgcc cggtccatgc ccgccgcgcc gggcgcgatg ccgcgaaaaa
     7921 tgttgccgcc gccgatcacc acgccgagct ccacgccgag gcgcgacacc tgcgccacct
     7981 cgcccacgat cgtctcgatc gtctgccggt tgatgccgaa cgggtcctcg cccatcagcg
     8041 cttcccccga cagcttgagc agaacccgtt tgtacttggg agtcaatgcg cctttccggc
     8101 ctgcgccatc acctcggcgg cgaaatcggt cgacttcttc tcaatcccct cgcccaccac
     8161 gaagaacgcg tagccgtgca ccttggcctt tttagcgacg agcattttct ccaccgtccg
     8221 cttgtcgtcg cgcacgaagg gctgtccgag cagcgttttc tcgttgagcg ctttgttgac
     8281 cgcgccctca acgatcttcg caatgatgtc ggcgggcttt ccggactcct tcgcgcgggc
     8341 ttcctggact ctccgctcgt cgagcacaat ctcctgaggg acctggacct tgctgagata
     8401 cgccggtttg gcgaaggcga tatgcatcgc gacgtccttg ccgacgtcct ccgggccctc
     8461 gtaatcgacc agaaccccga ttctgctccc ggcgtggatg tacagcgcga gcttgccctt
     8521 tgcctccatt cgcgtgaagc ggcggatggt catgttctcg ccgatcttct ggatgagcgc
     8581 ctggcgccga gcttccacct tgctaccgag aagatcgagc aagctcagct ctcccacgct
     8641 ggacggattg cgcatcccca cgatctcggc aagcgacttc gcgaaaccaa tgaaatcatc
     8701 gttcttggcg acaaagtcgg tttcgctgtt caactccacg agcgagccga ccttcccatc
     8761 ggccgacagc caggcgccga tcacgccttc ggcagcgatg cgatcggctg ccttggtggc
     8821 cttggcgctg cccttgatgc gcagcagctc ctcggccttt ttcagatcgc cgccggtctc
     8881 ggcgagcgct ttcttgcatt ccatcatcgg aagcccggtg cgttcgcgca gctccttcac
     8941 caggccggcg ctgatctctg ccattgcctc acgctccttc gggttgaaaa aaaggggccg
     9001 aagccccttt tgcggatatg catgcgaacc aatctactgc gaggcctcat cttcttccac
     9061 ctcgacaaac tcgtcgcgcg aggcggcgac gacctcctcc agggactggg tgcggccctc
     9121 gaggaccgcg tccgcgatgc cgcgggcata gagccgaatc gcgcggctcg agtcgtcgtt
     9181 tccgggaatg acgtagccga ttccctcggg gttatggttg gtgtcgacca caccgatgac
     9241 cggcacgccc agcttgttcg cctcggcgac cgcgcctttc tggtaaccga cgtcgatcac
     9301 aaagagcgca tcgggtagcg cgccgagatc cttgatgccg ccgagcgaac gctgcagctt
     9361 ggcgagctcg cgcgtgagcc cgagcgcctc cttcttggaa atccgctcga gactgccgtc
     9421 ctgctgcatc gcctccatct ccttgagccg cttgatggac tgcttcacgg tcttgaaatt
     9481 ggtcagcatg ccgccgagcc agcggtggtc gacgaagggc atctggcagc ggatcgcttc
     9541 ctcgcgcacg atatcgcgcg cctggcgctt agtgccgacg aacaggatcg ttccccggtt
     9601 cgccgtgagc tggcgagcga acttgagcgc ttcctggtaa agcgccaggg tcttttccag
     9661 gttgatgatg tggatcttgt tgcggtggcc gaagatgtac ggcgccatct tcgggttcca
     9721 gtaccgggtc tgatgcccga agtggacccc ggcttccagc atttgacgca tcgtcaccga
     9781 cactcacttc tccttagggt tgggtctgcc gcgcggctct gcccggacat ccgggaccct
     9841 tttcgagcct gcgggaagat tttgagcgcg aaatcatagc ataatcagtc tgatggccgt
     9901 agtcctgaag agcccggaag agctggggcg catgcgtgtc gccggtcggc tcgcctccga
     9961 ggtgctcgat tacatcaccc cgcacgtgcg agccggagtg agcaccggga agctgaacga
    10021 cctttgccac cactacatgg tggacgtgca gggaaccgtg cccgcgccgc ttaactacgc
    10081 gccgccgggc taccggccgt tcccgaagtc gatctgcacc tcggtgaacc accaagtctg
    10141 ccacggcgtg ccgggcgagc gcgtgctcaa gccaggcgac atcctgaaca tcgacatcac
    10201 cgtcatcaag gacggcttcc atggcgactg cagccgcatg ttctacgtgg gcgagccgag
    10261 catccaggcg aagcgcctgg tcgaggtgac ctttgaatgc atgtggctgg gcatccggca
    10321 ggtgcgcccg ggcgcgcgcc tgggtgacgt cggcgccgcg atccagagcc acgccgagag
    10381 gcacggcttc tccgtagtgc gcgagttctg cggccacggc attgggcgca gattccacga
    10441 agagccgcag gtcctgcact acggcaagcc gggcgtcggc gtggcgctcg agcccggcat
    10501 gacgttcacg atcgagccga tgatcaatgc cggcaagccg gggatccgcg agctcgccga
    10561 tggctggacc atagttacga aggaccacag cctttcggcc caatgggagc acaccgtcgc
    10621 cgtcaccgga tccggccacg aggtcctgac caagtccaac ggcactccgg cgccaccgtg
    10681 aagttcgatt tgcgcgccga ggtggcagcc gagcggcagg cgctgcgtga agcctacctg
    10741 cggcgcccga acccgcgtgc gctacttgcg aagcacacgc agctcgtaga ccgcaccgca
    10801 aaggccctct ggggcgaggc cgcagtggcg actgaagccg ccctcgtcgc caccggcggc
    10861 tacggccgcg gggagctctt ccctagctcc gacatcgatc tgctggtgct gcttggcacc
    10921 gatccgagcg aagccgagcg cgaagcgctc gagcgcctga tcggtcggct gtgggacatc
    10981 ggtctggaga ttgggcacag cgtgcgcacg gtggaaggtt gcgccgaggc ggcggccggt
    11041 gacgtcacca tccgcaccac gctgctcgag tcgcggtttt tggtcggcag ccgcgcgctt
    11101 ttccgccggc tcgagcaggc gcttgtgttc gacccggtcg cgttcttcaa ggcgaagaag
    11161 ctcgagcagg agcagcgcca tgccaagcac caggactcgc cctattcgct cgaacccaac
    11221 ctgaaggagg cacccggggg actgcgcgat ctccagatca tccagtggat cgcccgagcg
    11281 agcgggatgg gccgccgctg gacggacctt gtggcgcaca agctgatcca gcgcgacgag
    11341 gcgcggcagc tttcgcgcct cgagcgccac ctgcacgatc tcaggatccg cctgcattac
    11401 ctcgccggcc ggcgcgagga ccgcctcgta ttcgacctgc agacggcgct cgcaaagcag
    11461 ctcggctacg aggacacgcc ggcgcggctc gcaagcgaag cgatgatgca gcgctactac
    11521 cggactgcgc agggcgtcac gcagctcaac acgatcctcc tgcaaaatct cgaggcgcgg
    11581 cttgcgccgc ggcccgacac cgcgccgcgc ccgctgaacg agcgcttcga ggtaagaagc
    11641 gagcttctcc acatcaggca ggaagggacg ttcgcgcgca cgccaagcgc gattctcgag
    11701 tgcttcctgc tcatgatgca gcaccccgag ctgcgcggaa tgacggcgcg aacgctgcgc
    11761 gcgctgtggc gggcacgcag cctggtgaat ggaaaattca gagaggattc aatcgcaaga
    11821 ctattgtttt tgcacatcct gcagcagccg cgcgggatcg tgcacgagtt tcgccgcatg
    11881 aaccagtacg gcatcctcgg gcgctacctg cccgagttcg gccgtatcgt cgggcagatg
    11941 cagcacgatc tgttccacgt ctacacggtg gatcagcata tcctgatggt ggtgcgcaac
    12001 ctgcggcgct tcaccatgcc cgagttcgcg cacgagttcc ctctgtgcac cgagctcatg
    12061 gcgggattcg agcgccgctg gctcctttat gtcgctgcgc tctaccacga catcgccaag
    12121 ggtcgcggcg gcgaccattc cgagcttggc acgagagacg cgcgggcttt cgccagaaac
    12181 cacgggctgg cgcaacagga tcgcgaactc gtcgagtttc tcgtccagcg ccacctcgcc
    12241 atgtcgcatg tggcccaaaa gcaggacgtc tacgacccgg aggtggtgcg ggcgttcgcc
    12301 gatctggtgc gcaccgagcg gcggctcgtg gcgctgtatc tgttcaccgt ggccgacgtg
    12361 cgcggcacga gccccaaagt ctggaacgcg tggaaaggca agcttctcga ggatctgttc
    12421 cgcgcgaccc ggcgcgtgct caccggcgag ccgcttgcgc gcgatgccgc gcttgccgag
    12481 aagcagggcg aggccgcaag gctattgcgg ctctatgcgc tttcggacgc cgtcaaggaa
    12541 aagctctggg cgagcgtcga cacagcgtac ttcctgcgcc acgatgcgca agagatcgcc
    12601 tggcacacgc gcaacctgca ttaccgggtc gatactgaca agcccgtggt gaaggcgcgc
    12661 ctcgcgccct tcggcgaagg attgcaggtc atggtctaca cgcgtgaccg cgaggccctg
    12721 ttcgcacgca tctgcggtta cttcgagcgc gccggcttca gcatcgcgga agcgaaggtg
    12781 cacacgacgc gcaacggcta cgcgctcgat accttcgtcg tcatgggcga agggcgcgac
    12841 gcgcactatc aggaccgcat cgccatgatc gagaccgagc ttgccgccga ggtgcagtcc
    12901 ggcgctcccc tgggcccgcc gcgcggcggg cgcctgtcgc gccgggtgcg ccatttcccg
    12961 atctcgccgc gagtggacgt gcgccccgac gagcgcggtg cctaccacgt gctctcgatc
    13021 gtcgcagccg accggccggg cctgctctac ggcgtcgcgc gcattctggc ccgtaaccgg
    13081 atcaacctgc agaccgcccg catcaacacg ctcggcgacc gcgcggagga cgttttcctc
    13141 atctccggcg agtcgcttgg cgactcgaag acggtgttgc agctcgagca ggacctgctc
    13201 gaggagctgc agatctagcc ccgcgcctgc ggcgcgcggt aggcggcgag ctcggcgcgg
    13261 cgcttgcggg ccatcgcgtc gagggtcttc ttccagtccg cgcggcgccc acgcagcgcc
    13321 tccacgaccg attccgcgat aacgaggttg cggtaccact tggcgtccgc aggtacgact
    13381 gtccagggcg catgcggagc cgcggttttc gagatcgcgt cctcgtaggc ctcggtgtac
    13441 tcgtcccagt actccctctc cttccagtcg ttcgcgttca gcttccaggc agtcttcgga
    13501 tcgctctcgc gttcgagcag gcgcttcttt tgctcctgct tgctgatgtg gaggaa
//
LOCUS       DASTPD010000242         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_537224, whole genome shotgun sequence.
ACCESSION   DASTPD010000242 DASTPD010000000
VERSION     DASTPD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537224"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFR57_08050"
     CDS             complement(<1..990)
                     /locus_tag="VFR57_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0259352.1"
                     /translation="MQTRRFDAIVVGAGGSGMRAALELARANLKVAVLSKVFPTRSHT
                     VAAQGGIAAPLANTTEDNWHWHMYDTVKGADYLGDQDAIEYMCRRAIEVVVELEHMGM
                     PFDRIENGKIYQRPFGGHTQNYGTPKMAMRACAAADRTGHAMLHTLYQQNVRANTQFL
                     VEWMALDLIIDPQDGACVGVTALEMETGELFLLQAKATLLATGGAGRIYWATTNAFIN
                     TGDGLGMVARAGLPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGYLLNKNGERFMERY
                     APNAKDLASRDVVSRAMTTEIKEGRGCGPDRDYLELKLSHLKPEDIIRRLPGIR"
     gene            complement(990..1334)
                     /gene="sdhD"
                     /locus_tag="VFR57_08055"
     CDS             complement(990..1334)
                     /gene="sdhD"
                     /locus_tag="VFR57_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEU0259353.1"
                     /translation="MVKRLVVGAHYGLRDWLAQRITAALMVVYTLFAIGVVLKHQPIT
                     HAVWQDLFSRGWMRVPTLLFAVCLVWHAWVGVRDILMDYIKPDGLRLTLQVLFVLLLA
                     AYLGWAIQILWR"
     gene            complement(1328..1732)
                     /gene="sdhC"
                     /locus_tag="VFR57_08060"
     CDS             complement(1328..1732)
                     /gene="sdhC"
                     /locus_tag="VFR57_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0259354.1"
                     /translation="MASASAKKIRPKYLNLPAILFQIRLPVPGWVSILHRVSGALLVI
                     PFTAWALYMLDVSLRSEEGFQSIARYFELPLVKLGMLLFIWAYCHHFCAGIRYLFLDL
                     DKGIELRPARITSWIVIVAGLALTAVLGALLW"
     gene            complement(1787..>2224)
                     /locus_tag="VFR57_08065"
     CDS             complement(1787..>2224)
                     /locus_tag="VFR57_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259355.1"
                     /translation="RRGVVFDVGHGGGSFDYTIAEPAIAQGAPPDTISSDIHVFSGNT
                     PGMPYLTWVMSKFLNLGFTLEQVVAMATVNPARVIGRLPKLGTLELGAPGDVSILELV
                     EGPVRFVDTRKNERQGKVHLRPYQTVVAGVSFGRPYNAPFSVR"
BASE COUNT          401 a          716 c          735 g          372 t
ORIGIN      
        1 gcgaatgccc ggcaggcgcc ggatgatgtc ctcgggcttc agatgcgaga gcttgagctc
       61 gaggtagtcc cgatccggcc cgcagccgcg gccttccttg atctcggtgg tcatggcgcg
      121 cgacaccacg tcgcggctgg cgaggtcctt ggcgttcggc gcgtagcgct ccatgaagcg
      181 ctcgccgttc ttgttgagga ggtagccgcc ctcgccgcgc acaccctcgg tgatgagcac
      241 gcccgcgccc gccaccccgg tcggatggaa ctgccagaac tccatgtcct cgagcggcag
      301 ccccgcgcgc gccaccatgc ccaggccgtc gccggtgttg atgaaagcgt tggtggtcgc
      361 ccagtagatc cgtcccgcgc cgccggtcgc gagcaaggtc gctttcgcct gcaacaagaa
      421 gagctcgccg gtttccatct cgagcgccgt gaccccgacg caggcgccgt cctgcggatc
      481 gatgatgagg tcgagcgcca tccattcgac gaggaactgc gtattcgcgc gcacgttctg
      541 ctggtagagc gtgtgcagca tggcgtggcc ggtgcggtcg gccgctgcgc acgcgcgcat
      601 cgccattttc ggggtgccgt agttctgcgt atgcccgccg aaggggcgct ggtagatctt
      661 gccgttctcg atgcgatcga agggcatgcc catgtgctcg agctcgacca cgacctcgat
      721 cgcgcgccgg cacatgtact cgatggcgtc ctggtcgccg agatagtcgg cgcccttcac
      781 cgtgtcgtac atgtgccaat gccagttgtc ttcggtggtg ttcgcaagcg gcgcggcgat
      841 gccgccctgc gccgcgaccg tatgcgagcg cgtcgggaac acctttgaga gcaccgcgac
      901 cttgaggttg gcgcgcgcga gctcgagcgc cgcgcgcatg cccgagccgc cggcgcccac
      961 cacgattgcg tcgaatctgc gcgtctgcat cagcgccaga gaatctggat cgcccagccg
     1021 aggtaggcgg caagcagaag cacgaacaac acctggagcg taaggcgcaa gccgtcgggc
     1081 ttgatgtagt ccatcaggat gtcgcgcacg ccgacccacg catgccagac caggcacacg
     1141 gcgaagagca gagtcgggac gcgcatccag ccgcgtgaaa aaaggtcctg ccagaccgca
     1201 tgggtgatcg gctggtgctt gagcaccacg ccgatggcga acagcgtgta cacgaccatg
     1261 agcgccgcgg tgatgcgctg tgcgagccag tcgcgcaatc cgtagtgcgc gccgaccacg
     1321 agccgcttca ccaaagcagc gctcccagca ccgcggtgag cgcaaggcct gcgacgatga
     1381 cgatccagct cgtaatacgc gcgggtctta gctcgatgcc cttgtcgaga tccaggaaga
     1441 gatagcggat gcccgcgcag aagtgatggc aataggccca gatgaagaga agcatgccga
     1501 gtttcacgag cggcagctcg aaatagcgcg cgatcgactg gaagccttct tcgctgcgca
     1561 ggctcacgtc cagcatgtac agcgcccacg cggtgaacgg aatcacgaga agcgcgccgg
     1621 agacgcggtg caggatcgaa acccaccccg gcaccggcag gcggatctgg aacaggatgg
     1681 cgggcaggtt gaggtacttc ggacggattt tttttgcgga ggcgctcgcc atagaaagcg
     1741 cccgggcgct gcttttcacc tgtttccggg caattgatta tagcggtcag cgcacgctga
     1801 acggcgcgtt atacggtctc ccgaatgaaa cccccgcgac gacagtctgg tacgggcgca
     1861 ggtgcacctt gccctggcgc tcgttcttgc gcgtatcgac gaagcgaacc ggtccttcga
     1921 cgagctcgag gatggaaacg tcgccgggcg ccccgagctc gagcgttccg agcttcggca
     1981 atcgtccgat gacgcgcgcg ggattgaccg tcgccatggc gacaacctgc tccagcgtga
     2041 agccgaggtt caaaaatttg ctcatcaccc acgtgaggta cggcattccc ggcgtgttgc
     2101 cggaaaagac atggatgtcg gaggagatgg tgtcgggcgg cgccccctgc gcgatcgccg
     2161 gctccgctat cgtgtagtcg aagctgccac cgccgtggcc gacgtcgaac acgaccccgc
     2221 ggcg
//
LOCUS       DASTPD010000243         6924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_945042, whole genome shotgun sequence.
ACCESSION   DASTPD010000243 DASTPD010000000
VERSION     DASTPD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945042"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFR57_08070"
     CDS             complement(<1..279)
                     /locus_tag="VFR57_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU0259356.1"
                     /translation="MKPRHKRILAIVGGLAALGVATALVLTAFQENLVFFFTPSQVVA
                     NEAPQGRTFRIGGMVEKGSVQRSGIEVRFVVTDTAKSIPVVYSGALPDL"
     gene            complement(263..388)
                     /gene="ccmD"
                     /locus_tag="VFR57_08075"
     CDS             complement(263..388)
                     /gene="ccmD"
                     /locus_tag="VFR57_08075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEU0259357.1"
                     /translation="MSEFLAMGGYGWYVWGSYGVTFACLAIEVLLLWRRSRETEA"
     gene            complement(385..1128)
                     /gene="ccmC"
                     /locus_tag="VFR57_08080"
     CDS             complement(385..1128)
                     /gene="ccmC"
                     /locus_tag="VFR57_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEU0259358.1"
                     /translation="MRWFWYASPQTFFPLAGRLTRWSAAAAAVLAAAGLYIGFFVAPT
                     DAQQGEAYRIIFIHVPAAWMSMFIYVVMAFWAGVGLALNTRLSGMMAAALAPTGALFT
                     FIALWTGAFWGRPTWGTYWVWGDARLLSELILLFLYFGVMALRAAIDDPRRADRAAAV
                     LAIVGVVNIPIIYYSVQWWNTLHQGASVSLTAAPRMAATMLTGMLIMALAFWMYSIAV
                     TLMRVRCIIRERETPRSPAARDPLVRGSP"
     gene            complement(1125..1793)
                     /gene="ccmB"
                     /locus_tag="VFR57_08085"
     CDS             complement(1125..1793)
                     /gene="ccmB"
                     /locus_tag="VFR57_08085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU0259359.1"
                     /translation="MLAAARCVVYRDLLLAMRRPGDVATALLFFIIVASLFPLGIGAE
                     PNLLRAVAPGVIWVAALLSSMLSLARLFAADYDDGTLEQMLLGAAPLGVVVAAKAFAH
                     WLVSGLPLVAISPLVALQYDLSPALYGVLALSLLLGTPVLSLIGAIGAALILGTRGGG
                     VLLALLVLPLYVPVLIMGAGSVEMAEAGLGAAGQVLLLGAMLVVAAAFAPWAIAVALR
                     ISSE"
     gene            complement(1795..2400)
                     /gene="ccmA"
                     /locus_tag="VFR57_08090"
     CDS             complement(1795..2400)
                     /gene="ccmA"
                     /locus_tag="VFR57_08090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEU0259360.1"
                     /translation="MLEAAALECERGGRTLFRGLSFALRAGEALRVAGANGSGKTSLL
                     RILCGLTAPSAGEVRWQGSPIGALRENYARHLVYLGHAPAVKGDLTATENLAITCTLA
                     GLQIRKADLHTALERLGLAAHAVPVRKLSQGQRRRAALARLCVSAAPLWLLDEPFSAL
                     DAGGIDVLRTLVLEQLEHGGVVVFTTHQDPGIEATRVVDLG"
     gene            complement(2424..2993)
                     /locus_tag="VFR57_08095"
     CDS             complement(2424..2993)
                     /locus_tag="VFR57_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEU0259361.1"
                     /translation="MKQWIKRVGPWTIVGLFAAGIIFWGGFNWSFELTNTEPFCVSCH
                     EMQENVFKEYQHTVHYTNRSGVRATCPDCHVPKEWGPKIVRKIYASNELLHKVLGSID
                     TPEKFQAKRLKLAESEWLRMKANDSQECRNCHKFEYMDFSEQGRRAVRTHSRAFDEGE
                     TCIECHKGIAHQLPPIYQEIGAPKAVAAR"
     gene            complement(2990..3337)
                     /locus_tag="VFR57_08100"
     CDS             complement(2990..3337)
                     /locus_tag="VFR57_08100"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEU0259362.1"
                     /translation="MKPIRGKVPIPQTNPSDTYRMERHDRTIRRAHKWQPPIIPHHIR
                     GYQITKNVNTCMTCHSRKAAPGSGATQVGKSHYLDREGKEHPNISTRRYFCLQCHVPQ
                     FDAEPLVGNTYTP"
     gene            complement(3400..5868)
                     /gene="napA"
                     /locus_tag="VFR57_08105"
     CDS             complement(3400..5868)
                     /gene="napA"
                     /locus_tag="VFR57_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HEU0259363.1"
                     /translation="MKLDRRAFLKANAVAAAASVSGIPVKALAADAPKESDIKWDKAA
                     CRFCGTGCSVLVGTREGRVVATQGDPESEVNRGLHCVKGYFLSKIMYGADRLKAPLLR
                     KKNGVYDKNGEFTPVSWNEAFDVMAAKWKEALKAGGPTTVGMFGSGQWTIWEGYAAAK
                     LFKAGFRSNNLDPNARHCMASAVAGFMRTYGIDEPAGCYDDLEHADAFVLWGSNMAEM
                     HPVLWTRITDRRLMNPHVKVAVLSTYQHRGHELADLHMIFTPQTDLAILNFIAHHIIS
                     TGRVDKDFVGKHVNFALGNADIGYGLRPEDEREKRAKNAGDAEAMKPISLDEYAKFVS
                     EYSVEKVSKLSGVPARLLVELAELYADPKRKVVSLWTMGFNQHTRGTWVNQLCHNVHL
                     LTGKIAEPGNHPFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHREVAEKIWHLPA
                     GTIPEKPGYHAVMQNRMLKDGRLNAYWVQCTNNMQTAANLMQETWPGYRNPKNFVVVS
                     DPYPTVTAMAADLILPTSMWVEKEGAYGNTERRTQFWRQQVKAPGEAKSDVWQIVEFS
                     KRFKVEEVWPAELLKQKPEYRGKTLYDVLFANGQVNKFPGGDNDESRHFGFYLQKGLF
                     EEYAQFGRGKAHDLADFDTYHKVRGLRWPVVDGKETKWRFREGYDPYVKKGEGVRFYG
                     KPDGKAVVVAVPYEPPAEVPDKEYDLWLCTGRVLEHWHSGSMTRRVPELHRAQPEALV
                     FMHPNDAKKRRLLNGQEVRVMSRRGEVRARIETRGRNEPPEGLVFVPFFDEGRLVNKL
                     TLDATCPISRETDYKKCAVKVTKV"
     gene            complement(5855..6121)
                     /locus_tag="VFR57_08110"
     CDS             complement(5855..6121)
                     /locus_tag="VFR57_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEU0259364.1"
                     /translation="MRGRRQILGGDAVEIVSAIVSALPGQRERLRAAMLGLPGVEIHG
                     ETRDGRFIVTIESTGKASAGDTLLALHRLEDVLAAALVSHYEAR"
     gene            complement(6185..>6924)
                     /locus_tag="VFR57_08115"
     CDS             complement(6185..>6924)
                     /locus_tag="VFR57_08115"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU0259365.1"
                     /translation="GEAATGIANLIVSAMVEEGLAAPEARKRCWLFDSHGLVVASRRD
                     LAEHKRPFAHADQSIADFPSAVRELCPSAIIGVAAVGGTFTGEVLQDMARINRRPIVL
                     ALSNPTSKSECTAEQAYRYTDGRALFACGSPFDAVVLEDRTYVPRQGNNSYIFPGVGL
                     GVIASGARRVTDEMFMAAARTLAKLVTEEDLAQGSLYPALPRIREVSAEIGAAVAQVA
                     HRRGLATMTPPADVLAYVKAQMYNPRY"
BASE COUNT         1161 a         2332 c         2236 g         1195 t
ORIGIN      
        1 aaggtcgggc agcgcgccgc tgtacacgac cggaatgctc ttggcggtgt cggtgacgac
       61 gaaacgcacc tcgatgcccg agcgctgcac gctgcctttc tccaccatgc cgccgatacg
      121 gaaggtgcgg ccctgcggcg cctcattcgc cacgacctgc gaaggcgtga agaagaacac
      181 gaggttttcc tggaacgccg tcaggacgag cgcggtcgcc acgccgagcg cggccaggcc
      241 gccgacaatc gcgaggatgc gcttatgcct cggtttcacg gcttcttctc cacaacaaga
      301 gcacttcgat cgccaggcag gcaaatgtca ccccgtacga tccccacacg taccaaccgt
      361 acccgcccat cgcaagaaat tcgctcatgg cgaccccctg acaagggggt cgcgagccgc
      421 aggcgagcgg ggggtttcgc gctcccggat gatgcagcgc acgcgcatga gcgtaaccgc
      481 gatgctgtac atccagaaag ccagcgccat gatcagcatt ccggtgagca tcgtagcggc
      541 catcctgggc gcggcggtga ggctcactga cgcgccctgg tgcagcgtgt tccaccactg
      601 caccgagtag tagatgatgg ggatgttcac cacgccgacg atcgctagca ccgccgccgc
      661 ccggtcggcg cgccgcggat cgtcgatcgc cgcgcgcagc gccatcacgc cgaagtagag
      721 gaagagcagg atcagctccg acaggagacg cgcatcgccc cagacccagt acgtgcccca
      781 ggtcggccgt ccccagaacg cgccggtcca cagcgcgatg aacgtgaaga gcgccccggt
      841 gggcgcgagc gccgcggcca tcatccccga aagccgcgtg ttgagcgcga gcccgacccc
      901 cgcccagaac gccatcacca cgtagataaa catcgacatc cacgccgccg gcacgtggat
      961 gaagatgatg cggtaggcct cgccctgctg cgcgtcggtg ggtgcgacga agaagccgat
     1021 gtagagcccg gcagccgcga gcaccgcggc cgcggcggcg ctccagcgcg tgagccttcc
     1081 cgcgagcggg aagaaagtct gcggcgaggc gtaccagaac catctcattc cgacgaaatc
     1141 ctcagggcca cggcaatcgc ccagggggcg aacgcggcgg cgacgacaag catggcgccg
     1201 agcagcagca cctgaccggc tgcgcccagc ccggcttccg ccatctcgac gctccccgcg
     1261 cccatgatca gcactggaac gtagagcggc aagaccagca gagcgagcag gacgccgccc
     1321 cctcgcgttc cgagaatgag cgcggcgcca atggcaccga tcaggctcag caccggcgtg
     1381 ccaagcagca atgataaggc aagcacgccg tagagcgcgg gcgagaggtc gtactggagc
     1441 gcgacgagcg gcgagatcgc gacgagtgga agccccgaca ccagccagtg cgcgaacgcc
     1501 ttcgccgcca cgacgacgcc gagcggtgcg gcgccgagca gcatctgctc gagcgtaccg
     1561 tcgtcgtagt cggcggcgaa gaggcgcgcg agcgacagca tggacgacag cagtgccgcg
     1621 acccagatca cgcccggcgc gaccgcgcgc agcaggtttg gctcggcgcc gataccgagg
     1681 gggaacaggc tcgccacgat gatgaagaaa aggagcgcgg tggccacgtc accgggccgc
     1741 cgcatggcga gcagcaggtc gcggtacacg acgcagcgag ccgcggcgag cacgtcagcc
     1801 gaggtccacc acgcgcgttg cctcgatgcc cggatcctgg tgcgtggtga agacgacgac
     1861 tcctccgtgc tcgagctgct cgagcacgag cgtcctcagc acgtcgatgc cgcccgcgtc
     1921 gagcgccgag aacggctcgt cgagcagcca cagcggagcc gccgacacgc agagccgcgc
     1981 gagcgccgcc cggcggcgct gaccttgcga gagcttcctt accgggaccg cgtgcgccgc
     2041 cagcccaagg cgctcgagcg cggtatggag gtcggccttg cgtatttgca gacctgcgag
     2101 cgtgcatgtg atcgccagat tctcggtcgc ggtgaggtcg ccctttaccg ccggggcatg
     2161 gccgagatag accagatgcc tcgcatagtt ctcccgcagc gctccgatcg ggcttccctg
     2221 ccatcgcact tcgcccgcgc tgggcgccgt gagcccgcac aggatgcgaa gcaggctcgt
     2281 cttgccgctg ccgttggcgc cggcgacgcg cagcgcttca cccgcgcgca gcgcgaacga
     2341 cagtccgcgg aacagcgttc ttccgccacg ctcgcattcg agagccgccg cctccagcac
     2401 atggttattg ttgcagaaac cggctagcgc gccgccacgg cctttggagc gccgatctcc
     2461 tggtagatcg ggggcagctg gtgcgctatc cccttgtggc actcgatgca ggtctcgccc
     2521 tcatcgaacg cccggctgtg ggtgcgcacc gcgcgccgcc cctgctccga gaagtccatg
     2581 tactcgaact tgtggcagtt gcggcattcc tgggaatcat tcgccttcat gcgaagccat
     2641 tcgctctcgg caagcttcag gcgcttcgcc tggaacttct ccggcgtgtc gatgctgccg
     2701 agcaccttgt gcagcagctc gtttgacgcg tagatcttgc gtacgatctt cggcccccat
     2761 tctttcggca cgtgacagtc cgggcaggtc gcgcgcactc cggagcggtt ggtgtaatgc
     2821 acggtgtgct gatattcctt gaacacgttc tcctgcatct catggcacga gacgcagaac
     2881 ggctcggtgt tggtgagctc gaacgaccag ttgaacccgc cccagaaaat gatccccgcg
     2941 gcgaacaggc ccacgatggt ccacggcccg acgcgcttga tccattgctt catggcgtgt
     3001 aggtattccc taccagcggc tcggcgtcga attgcggcac atggcactgc aagcagaagt
     3061 agcgccgcgt cgagatattg ggatgctcct tgccttcgcg atcgagatag tgcgacttgc
     3121 cgacctgcgt ggcgccgctc ccgggcgcgg ccttgcggct gtggcaggtc atgcaggtat
     3181 tcacgttctt ggtgatctga tagccccgga tgtgatgcgg gatgatcggc ggctgccatt
     3241 tgtgcgcgcg ccggatcgtg cggtcgtggc gctccatgcg gtaggtatcg gacggattgg
     3301 tctgcgggat cggcaccttc ccgcgaatgg gcttcactac ttcttcctgt gaaaaagtca
     3361 gcgtgaaggt caaggccagc acaacagcga ggaggagcct catacctttg tgaccttgac
     3421 ggcgcacttc ttgtagtcgg tctcgcgcga gatcgggcag gtggcgtcca gggtgagctt
     3481 gttgacgagg cgcccttcgt cgaaaaacgg cacgaacacc agcccctcgg gcggctcgtt
     3541 gcggccgcgg gtctcgatgc gcgcgcggac ctcgccgcgg cgcgacatca ccctgacttc
     3601 ctggccgttg aggagccttc tcttcttcgc gtcgttcgga tgcatgaaaa cgagcgcctc
     3661 gggctgcgcg cggtggagct ccggcacgcg ccgggtcatg gagccggagt gccagtgctc
     3721 gagcacgcgc ccagtgcaca gccacaggtc gtattccttg tccggcacct cggccggcgg
     3781 ctcgtagggc acggcgacga cgaccgcttt cccgtccggc ttgccgtaga agcgcacacc
     3841 ctcgcccttc ttcacgtacg ggtcgtagcc ctcgcggaag cgccacttcg tttccttgcc
     3901 gtcgaccacc ggccagcgca gcccgcgcac cttgtgatat gtgtcgaagt cggccaggtc
     3961 gtgcgccttg ccgcggccga actgcgcgta ttcctcaaac aggcccttct gcaggtagaa
     4021 cccgaaatgg cgcgattcgt cgttgtcgcc gcctggaaac ttgttcacct gcccgttggc
     4081 gaaaaggacg tcgtacagcg tcttgccacg gtattcgggc ttctgcttca gcagctccgc
     4141 tggccacacc tcctcgacct tgaagcgctt ggagaactcg acgatctgcc agacatcgga
     4201 cttcgcctcg cctggcgcct ttacctgctg gcgccagaac tgggtgcgcc gctcggtgtt
     4261 gccataggcg ccctccttct ccacccacat cgaggtcggg agaatgaggt cggcggccat
     4321 cgcggtcacc gtcggatacg ggtcgctcac gacgacgaag ttcttcgggt tgcggtagcc
     4381 gggccaggtc tcctgcatca ggttggccgc ggtttgcatg ttgttcgtgc actgcaccca
     4441 gtaagcgttc agcctcccgt ccttcagcat ccggttctgc atcaccgcgt gatagccggg
     4501 cttttccggg atcgttcccg cgggcaggtg ccagatcttc tccgcgacct cgcggtgctt
     4561 cgggttcgcg actaccatgt cggccggcag ccggtgcgag aaagtgccga cctcgcgcgc
     4621 cgtgccgcat gccgagggct ggccggtgag cgagaatgga tggtttcccg gctcagcgat
     4681 cttgccggtg agaaggtgga cgttgtggca gagctggttc acccaggtgc cgcgcgtgtg
     4741 ctggttgaag cccatggtcc agagcgacac caccttgcgc ttcgggtcgg cgtacagctc
     4801 cgcaagctcg accagcagcc gcgcgggaac gcccgagagc ttcgatacct tttcgacgct
     4861 gtattcagaa acaaacttgg cgtattcgtc gagcgagatc ggcttcatcg cctcggcatc
     4921 gcccgcgttc ttcgcgcgct tctcgcgctc gtcctcgggc cgcaagccgt agccgatgtc
     4981 ggcgttgccg agcgcgaaat tcacgtgctt gccgacaaag tccttgtcga ccctgccggt
     5041 cgagatgatg tggtgggcga tgaagttgag gattgcgagg tcggtctgcg gcgtgaaaat
     5101 catgtgcagg tcggcgagct cgtggccgcg gtgttgatag gtcgagagca ccgccacctt
     5161 cacgtggggg ttcatgagcc ggcggtccgt gatgcgcgtc cacagcaccg ggtgcatctc
     5221 ggccatgttc gagccccaga gcacgaacgc gtcggcgtgc tcgaggtcgt cgtagcaccc
     5281 tgccggctcg tcgatgccgt aagtacgcat gaacccggcg accgccgagg ccatgcaatg
     5341 gcgcgcgttc ggatcgaggt tgttcgaccg gaagcctgcc ttgaagagct tcgccgcggc
     5401 gtagccctcc cagatcgtcc attgccccga gccgaacatg ccgaccgtgg tcgggccgcc
     5461 tgccttcagc gcttccttcc acttcgccgc catgacgtcg aaggcttcgt tccacgagac
     5521 cggcgtgaac tcgccattct tgtcgtagac gccgttcttc ttgcgcagca ggggcgcctt
     5581 cagccggtcg gcgccgtaca tgatcttcga caggaagtaa cccttaacgc agtgcagccc
     5641 gcggtttacc tccgactcgg gatcgccttg ggtcgccacc acgcgcccct cgcgcgttcc
     5701 cacgagaacg ctgcagcccg tgccgcagaa gcggcacgcc gccttgtccc atttgatatc
     5761 gctttccttg ggcgcgtcgg ccgcaagcgc ctttacgggg atgcctgaaa ccgaggcggc
     5821 cgcggccacg gcgttagcct tgaggaaggc gcgcctatcg agcttcatag tgcgagacga
     5881 gcgccgcggc gagcacatcc tccagccgat gcaaggcgag gagcgtgtcg ccagcgctcg
     5941 cctttccggt cgattcgatc gtcacgatga accggccgtc gcgcgtctcg ccgtggatct
     6001 ccacgcccgg caggccgagc atcgcagcgc gcaaccgctc gcgctggccc ggcagcgccg
     6061 agacgatcgc actgacgatc tctacggcgt ctccgccgag aatctgcctc ctgccccgca
     6121 tcggcgcatt gcaccagagc gctgctcgac cagcttgatc tgtatcaaat ctcgttgatc
     6181 ctgatcaata ccgtgggttg tacatctgcg ccttcacata ggcgagcacg tcggcaggcg
     6241 gcgtcatggt ggcgagcccg cggcggtgcg ccacctgcgc cacggctgcg ccgatttcgg
     6301 ccgagacttc gcggatgcgc ggcagcgccg gataaaggct tccttgcgcg aggtcctctt
     6361 cggtgacgag cttcgccaac gtgcgtgccg ccgccatgaa catctcgtcg gtgacgcgcc
     6421 gcgcgccgct cgcgatcacc ccgaggccga cgccggggaa gatgtacgag ttgttgccct
     6481 gccgcggtac ataggttctg tcctcaagaa ccactgcatc aaatgggctc ccgcaggcga
     6541 agagcgcccg cccgtcggtg tagcggtagg cctgctccgc ggtgcactca gacttggagg
     6601 tggggttcga gagcgccaga acgatcgggc gccggttgat gcgcgccatg tcctgcagca
     6661 cctcgccggt gaacgtgccg ccgaccgcgg cgacgccgat aatcgcgctc gggcacagtt
     6721 cgcgtaccgc gctcgggaag tcggcgatcg actggtcggc gtgcgcgaat ggccgcttgt
     6781 gctcagcgag gtcgcgccgg ctcgctacca ccaggccgtg cgaatcgaac agccagcagc
     6841 gcttgcgcgc ctccggcgcc gcgaggccct cctcgaccat tgcagagacg attagattgg
     6901 cgatcccggt cgccgcctcg ccgg
//
LOCUS       DASTPD010000244         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_537342, whole genome shotgun sequence.
ACCESSION   DASTPD010000244 DASTPD010000000
VERSION     DASTPD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537342"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFR57_08120"
     CDS             complement(<1..865)
                     /locus_tag="VFR57_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259366.1"
                     /translation="MKRRQFNQALAATAALAAVPYVRAQAQKLRVGVVLPKSGVQGFI
                     GQSCQKGAELAPGVIKEMLGVDIEIMSADNESNVDTSRTRTERLVQEGAHVLVGAFDS
                     GQTAAMAQVAEQRGIPHVINVAAAPQITEQGYKFVFRNFQTAPDIVREGLALIGELFQ
                     ATNTVPRTAVFMHVNDTFGQAMAKGIGAFMPRLTQLPFKIVDTISYDVAAKDLAVEVS
                     KAKASNADFLLLVCRLNDAILLRREMVKQRWNPMGIISPGSPGMYEEQYFKTLGKLSE
                     HSISNVPWYNPN"
     gene            923..1435
                     /locus_tag="VFR57_08125"
     CDS             923..1435
                     /locus_tag="VFR57_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0259367.1"
                     /translation="MRKRRFAARNLARRTSLPRGPRPSSVVRVTGIDYLDLDQHLGYA
                     LRRAQVAAFDAFHRATAGAGITPPRFTALVILQANPGISQSILGEVLGTARSGAMLLT
                     DWLEAQDLAERRHRSDDGRAWGLYLTGKGQRLIQRLRRRVRAYDRRFAARIRPGERRK
                     LLRLLEKLAR"
     gene            1432..3138
                     /locus_tag="VFR57_08130"
     CDS             1432..3138
                     /locus_tag="VFR57_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEU0259368.1"
                     /translation="MSRRQRAVRLGPRDVRVERRAGGVVHLCSPHAVPPFPSKITKRL
                     EYWAKAAPDRVFLAQRAGEDWRRLTYKDALERARRVGQYLLTKELSVERPLVVLSGND
                     IEHALLHLGAMYVGIPYAPISPAYSLLSTDFAKLRAILDLLTPGLAFVSDKPVFSKAL
                     NVVSCEVIDKLQEPQPTPAVDKAHASVGPDTIAKFLFTSGSTGTPKAVINTQRMWCSN
                     QAMILSALPFFEDEPPVLLDWAPWHHTAAGNHDLGLALYNGGSYYIDKGRPLPAEIET
                     TVRNLREVAPTWYFNVPKGFEMLLPFLKKDPQLRAHFFSRLKVLWFAGAALPQHVFDE
                     MKELAFRACGEEIAFLTGFGATETAPATLARVWPAENAANMGLPPPGAELKLVPMEGR
                     YEMRVKGPHVTPGYWRAPDLTERAFDAEGYYCIGDTFTFDHGEPASGLLFAGRIAEDF
                     KLLTGTWVHVGELRARFIRHFAPLVRDVVFAGEGRSDIGALIFLAGKASPEQLRDKLA
                     SFPNSGSSNRIAYAMVLEEPPSLDAGEMTDKGSINQRAVLASRKQLVEELFSDSERIL
                     RA"
     gene            3135..>3316
                     /locus_tag="VFR57_08135"
     CDS             3135..>3316
                     /locus_tag="VFR57_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259369.1"
                     /translation="MNVKDFVAIDVHTHAWKSALRVDDKLTDSQEAMGRYFRYQPQHQ
                     TVPEMAEMYRKLRMAFV"
BASE COUNT          557 a         1100 c         1075 g          584 t
ORIGIN      
        1 cgttcgggtt gtaccaggga acgttggaga tcgagtgctc cgaaagcttg ccgagcgtct
       61 tgaaatactg ctcctcgtac atgccgggcg atcccgggct gatgatgccc atcgggttcc
      121 agcgctgctt caccatctcg cgtcgcagca ggatcgcgtc gttcagccgg cacacgagca
      181 gcaggaaatc cgcgttcgaa gccttggcct tcgacacctc gacggcgagg tccttggcgg
      241 cgacgtcgta ggagatggtg tccactatct tgaacggtag ctgggtgagc cgcggcatga
      301 acgcgccaat gcccttcgcc atggcctggc cgaaagtgtc gttcacgtgc atgaataccg
      361 ccgtgcgcgg cacggtgttc gtcgcctgga agagctcgcc gatcagcgcg aggccttcgc
      421 gcacgatgtc gggcgcggtc tggaaattgc ggaatacgaa cttgtagccc tgctcggtga
      481 tctgcggtgc ggcggcgacg ttgatgacgt gcgggatacc gcgctgctcg gcgacctgcg
      541 ccatcgcggc ggtctggccg gagtcgaacg cgccgaccag cacgtgcgcg ccctcttgga
      601 ccaggcgctc ggtgcgcgtg cgggaggtat ccacgttgga ctcgttgtcg gcgctcatga
      661 tctcgatgtc gacgccgagc atctctttga tcacgccggg ggccagctcg gcccccttct
      721 ggcacgactg cccgatgaag ccctgcacgc ctgatttcgg cagcaccacg ccgacgcgca
      781 gcttctgcgc ctgggctctg acatagggaa ccgcggcaag cgcggccgta gcggcgagcg
      841 cttggttgaa ctgacggcgt ttcatcaccc ctcctccaga gtatgagcga tattgttata
      901 taggactaca atctcccggt caatgaggaa gcgccgcttt gccgcgcgta acctggcgcg
      961 gcggacctca ctcccccgcg gcccgcgccc gagcagcgtc gtgcgcgtga ccggcatcga
     1021 ctacctggac ctggaccagc acctgggcta cgcactgcgc cgcgcccagg ttgccgcgtt
     1081 cgatgcgttc caccgggcga cggcgggcgc tggcatcacg ccgccgcgct tcacggcgct
     1141 cgtcattctg caggcgaacc cggggatcag ccagtcgatt ctcggcgaag tgctcggcac
     1201 ggcgcgctcg ggcgcgatgc tcctcaccga ctggctcgaa gcgcaggacc tcgcggagcg
     1261 gcgccaccgc agcgacgacg ggcgcgcctg gggcctgtac ctcaccggca agggacagcg
     1321 cctcatccag cggctgcgac gccgggtgcg cgcgtacgac cgccgtttcg cggcgcgcat
     1381 ccgccccggc gagcgccgca agctgctgcg gctcctggag aagctcgcgc gatgagccgg
     1441 cggcagcgcg ccgtccgtct cgggccacgc gacgtgcggg tcgagcgccg cgcgggtggc
     1501 gttgtgcatc tctgctcccc ccatgccgtt ccgccttttc caagcaagat cacaaaaaga
     1561 ctggagtact gggcgaaggc tgcgccggat cgcgtcttcc ttgcccagcg cgcgggcgag
     1621 gactggcggc gccttaccta caaggacgcg ctcgagcggg cgcggcgcgt aggccaatac
     1681 ctgctgacga aagagctttc cgtcgaacgc ccgctcgtgg tgctttcggg caacgacatc
     1741 gagcatgcgc tcctgcacct cggcgcgatg tacgtcggca ttccgtatgc gccgatctcg
     1801 ccggcgtact cgctgctttc caccgacttc gccaagctgc gcgcgatcct agacctgctg
     1861 acaccggggc tcgcgtttgt ttccgacaag ccagtctttt ccaaagcgct gaacgttgtc
     1921 agctgcgaag tcatcgacaa gctccaggaa ccgcagccga ctccggccgt ggacaaggcg
     1981 cacgcgagcg ttgggcccga taccatcgcg aagtttctgt tcacgtccgg atccaccggc
     2041 acgccgaagg cggtgatcaa cacgcaacgc atgtggtgct cgaaccaggc gatgattctg
     2101 tcggcgctgc cgttcttcga ggacgaacct ccggtgctac tcgactgggc gccctggcat
     2161 cacaccgcgg cgggtaacca cgacttgggc ctcgcgctct ataacggcgg ctcgtactac
     2221 atcgacaagg gcaggccgct gccggcggag atcgagacca cggtgcgcaa cctccgcgaa
     2281 gtcgccccta cctggtactt caacgtgccg aagggcttcg agatgctctt gccgtttctt
     2341 aagaaagatc cccaactgcg cgcccatttt ttcagccggt tgaaagtgct ctggttcgcc
     2401 ggcgctgcgc tgccgcagca cgtgttcgac gaaatgaagg agcttgcatt ccgcgcctgc
     2461 ggcgaggaaa tcgctttcct caccgggttc ggcgccaccg agaccgcgcc cgcgacgctc
     2521 gcgcgcgtgt ggcccgccga gaacgcggcg aacatgggat tgccgccgcc gggggccgag
     2581 ctcaagctcg tccccatgga aggcaggtac gaaatgcgcg tgaaggggcc ccacgtgacg
     2641 cccggctatt ggcgtgcgcc ggacctcacg gagcgagcgt tcgacgccga aggttactac
     2701 tgcatcggcg acacgtttac cttcgaccac ggcgagccgg cgagcggact gctctttgcc
     2761 ggacgcatcg ccgaggactt caagctcctg accggtactt gggtgcacgt cggcgagctg
     2821 cgcgcgcgct tcatccggca cttcgcgccc ctcgtgcgcg acgtggtatt cgctggagag
     2881 ggccgcagcg acatcggcgc tctcatcttt cttgccggca aggcgtcacc cgagcagctg
     2941 cgggacaaac tggcttcgtt tccgaacagc ggcagctcca accgcatcgc gtacgcgatg
     3001 gtcctggaag agccgccctc gctggacgcg ggcgagatga ccgacaaggg atcgatcaac
     3061 cagcgggccg tcctcgcaag caggaagcaa ctggtggagg agcttttctc ggactcggag
     3121 cgaatattgc gcgcatgaac gtcaaggatt tcgttgccat cgatgtgcat acccacgcct
     3181 ggaagtcggc gctgcgggtg gacgacaagc tcaccgattc gcaggaggcg atggggcgct
     3241 atttccgcta ccagccgcag caccagacag tgcccgagat ggctgagatg tatcgcaagc
     3301 tcaggatggc attcgt
//
LOCUS       DASTPD010000245         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1762888, whole genome shotgun sequence.
ACCESSION   DASTPD010000245 DASTPD010000000
VERSION     DASTPD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762888"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2355)
                     /locus_tag="VFR57_08140"
     CDS             complement(<1..>2355)
                     /locus_tag="VFR57_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259370.1"
                     /translation="GSGDDCFSPEKNPALKREIKLARRSMVPDNYIKRVIQFAKQGYK
                     DINFPTYDTDWDSDAYLTVSGQNSNNSVRVTDHFLKAVEADGKWDLTYRKNGKVAETL
                     DARALWEKIGYAAWASADPGLQYHTTVNDWHTCPTSGEIRASNPCSEYMFLDDTACNL
                     ASLNLLTFRDEKTGKFQVESYEHGVRLWTVVLEISVMMAQFPSKQIAERSYEFRTLGL
                     GYANIGGLLMTSGIPYDSDAGRAICGALTAIMTGVSYATSAEMAEKLGPFPGYKKNRE
                     HMLRVMRNHRRAACGERSGYEKVATHPVPLDNASCPDQTLIAHARAAWDRVLALGEKH
                     GYRNAQATVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPEALRT
                     LGYSEADIAEIEAYAVGHGTLSQAPAINHTTLKAKGFTDEAIAKIEKALPTAFDIKFA
                     FNKWTLGEDFCRDKLGIPAEDLASPTFDLLAALGFGKREIEAANIHVCGAMTVEGAPH
                     LKAEHYPVFDCANPCGRTGKRYLSVESHIRMMAAAQPFISGAISKTINMPNEATVEDC
                     KAAYLLSWKLALKANALYRDGSKLSQPLNAQLIADEDDEDDAVEAFLERPAAARAAGL
                     AEKVVEKIVERVTVLRERERMPDRRKGYTQKAVVGGHKVYLRTGEYDDGRLGEIFIDM
                     HKEGAALRSIINNFAIAVSLGLQYGVPLEEYVDAFTFTRFEPQGPVQGNDSIKYATSI
                     LDYVFRELAVSYLERFDLAHVDPSEAGGFDALGKGVEEGKPAPAT"
BASE COUNT          381 a          694 c          809 g          471 t
ORIGIN      
        1 ggtcgcgggc gccggcttgc cttcctccac gcccttgccg agcgcgtcga agccgccggc
       61 ttcgctcggg tcgacatgcg cgagatcgaa ccgctccaga taggagacgg cgagctcgcg
      121 gaacacgtag tcgaggatcg aagtcgcgta tttgatcgag tcgttgccct gcaccgggcc
      181 ctgcggttcg aagcgggtga aggtgaaggc gtcgacatat tcctcgagcg gcacgccata
      241 ttgcaggccg agcgaaaccg cgattgcgaa attgttgatg atggagcgca gcgccgcgcc
      301 ttccttgtgc atgtcgatga agatctcgcc gagccggccg tcatcatatt cgccggtgcg
      361 gaggtagacc ttgtgaccgc cgacgaccgc cttctgggta tagcccttgc ggcggtcggg
      421 catgcgctcg cgctcgcgca gcacggtgac acgctcgacg atcttctcga cgactttttc
      481 cgcgaggccg gctgcgcgtg ccgcggcggg acgctcgagg aaggcctcga ccgcatcgtc
      541 ctcatcatcc tcatcggcaa tgagctgcgc gttgagcggc tgggagagct tggaaccgtc
      601 gcggtagagc gcgttggctt tcagcgccag cttccatgaa agcaggtagg ccgccttgca
      661 gtcttcgacc gttgcctcat tcggcatgtt gatggtcttg gagatggcac ccgagatgaa
      721 gggctgcgcc gccgccatca tgcgaatgtg gctctcgacc gagagatagc gcttgccggt
      781 gcggccgcac ggattggcgc agtcgaacac cgggtagtgc tcggctttga ggtgaggagc
      841 gccttcgacc gtcatcgcgc cgcagacatg gatgttggcg gcctcgatct cgcgtttgcc
      901 gaagccaagc gcggcgagga ggtcgaaggt gggcgaggca aggtcctcgg cgggaatgcc
      961 gagcttgtcg cggcagaagt cttcgcccag cgtccacttg ttgaaggcga acttgatgtc
     1021 gaaggccgtc ggcagcgcct tctcgatctt ggcgatcgct tcgtcggtga agcccttggc
     1081 cttgagcgtg gtgtggttga tcgccggcgc ctgcgacagc gtgccgtggc cgaccgcata
     1141 ggcctcgatc tcggcgatgt cggcttcgct gtagccaagc gtgcgcagcg cttccggcac
     1201 cgcgcggttg atgatcttga agtagccgcc gccggcaagc tttttgaact tcaccagcgc
     1261 gaagtcgggc tcgatgccgg tggtgtcgca atccatcacc aagccgatgg tgccggtggg
     1321 cgcgatgacc gttgcctggg cgttgcggta gccgtgcttc tcgccgaggg cgaggacgcg
     1381 gtcccaggcc gccctggcat gtgcgatcag cgtctgatcg gggcaggagg cattgtcgag
     1441 cggcacgggg tgcgttgcga ccttctcgta gccgctgcgt tcgccgcagg cggcgcggcg
     1501 atggttgcgc atcacgcgca gcatatgctc gcggttcttc ttgtagccgg ggaaaggccc
     1561 gagcttttcg gccatttcgg ccgaggtcgc gtaggacacg ccggtcatga tcgcggtcag
     1621 cgcgccgcag atggcgcggc cggcatcgga atcatacggg atgccggagg tcatcagcag
     1681 gccgccgata ttggcatagc cgagaccgag cgtgcggaat tcgtaggagc gttcagcgat
     1741 ctgcttggac gggaactgcg ccatcatcac cgagatttcg aggacgacag tccacagtcg
     1801 gacgccgtgt tcgtaactct cgacctggaa cttgccggtc ttctcgtcgc ggaaggtgag
     1861 caggttcaac gacgccagat tgcaggcggt gtcgtcgagg aacatgtatt ccgaacacgg
     1921 attgctggcg cggatctcgc ccgaggtcgg gcaggtgtgc cagtcgttga ccgtggtgtg
     1981 atattgcagc cccggatcgg ccgaagccca cgcggcgtaa ccgatctttt cccagagcgc
     2041 gcgcgcatcg agcgtttccg cgaccttgcc gttcttgcgg taggtgaggt cccacttgcc
     2101 gtcggcctca accgccttaa ggaaatggtc ggtgacgcgc acggaattgt tcgagttctg
     2161 ccccgagacg gtgagatagg cgtcggagtc ccagtcggta tcgtaggtcg ggaagttgat
     2221 gtctttgtag ccctgcttgg cgaactgaat cacgcgcttg atgtagttgt cgggcaccat
     2281 cgagcgacgc gcgagcttga tctcgcgctt gagcgccggg ttcttttccg gcgagaagca
     2341 gtcgtcgccc gagcc
//
LOCUS       DASTPD010000246         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_127367, whole genome shotgun sequence.
ACCESSION   DASTPD010000246 DASTPD010000000
VERSION     DASTPD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127367"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFR57_08145"
     CDS             complement(<1..313)
                     /locus_tag="VFR57_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259371.1"
                     /translation="MHGFLCSHEDWRLQLKELKQKHEVVACDLRGHGSTPGRPHECSI
                     EHFGGDVIALANNLELPPFILIGHSMGCRVVLEANRIAPERVAGIVLIDGSRAGSGDP
                     DG"
     gene            complement(361..2412)
                     /gene="fusA"
                     /locus_tag="VFR57_08150"
     CDS             complement(361..2412)
                     /gene="fusA"
                     /locus_tag="VFR57_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0259372.1"
                     /translation="MAHSIKRIRTMALVGQSGAGKTTLAEALLATAGAIPAAGSVERG
                     TTVCDYTPLEKQLQHSLKLAVASFDFQDHRIHLLDTPGYPDFLGHALSAFAAVETAAI
                     VINAQNGLEMMTGRFLHWAAKRGLDRLIIVNKIDADNVDLEGLLERIQQAFGKECLPL
                     NLPAGNRSKVSDCFFAPSGEADFSSVGEAHGKLVDQVVEVDEKLMEHYLEKGEVSPEQ
                     LHEPLERALREGHLIPVVFTSAKTGIGVAELLDVIVKLLPNPTEGNPPSYVVTKAGNG
                     QGSDLQPVPDPGKHALAHVFKIEIDPYIGRVAVFRVHQGRLTAGSQLYIGEGRKPIKA
                     GHLYLLRGKTQVEVHEAIPGDICAIAKIDEIQFDHILHDSAEDANVHAKPLDLPTPVF
                     GLAVQPKKRGDEQKVSDVLHKILAEDPCFRIEHNSQANETVMRGLGEMHLKAVLEKMS
                     SQYKLDLDTRPPSIPYRETIAARAEGHSRHKKQTGGAGQFGEVFLKVEPLPRGKGFEF
                     VDSVKGGAIPNQFIPSVEKGVRAVLDSGFLAGYPLQDVRVIVYDGKSHPVDSKDVAFQ
                     SAGRKAFLDALAKARPIVLEPIVNVEIVAPEDRMGDIAGELSGHRGQIKGSDSPRPGL
                     VQITAQAPLSELEHFPARLKSLTAGHGSYSLEFSHYDPAPPQLQQKLVSAHKPAASAE
                     E"
     gene            complement(2442..2882)
                     /locus_tag="VFR57_08155"
     CDS             complement(2442..2882)
                     /locus_tag="VFR57_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEU0259373.1"
                     /translation="MALDPHLPEIAGVIQLAIAPVFMLTALGTVINALNLRLGRAVDR
                     RRELEERLAAMSQAELPSAQDELATIARRIRFVYLAIVFAVISALFTALLIAGAFVGA
                     FVRTDLSFTIAGMFVCAMVALIVCLLLFLREIFLAVSTPRHVPR"
     gene            complement(2909..>3734)
                     /locus_tag="VFR57_08160"
     CDS             complement(2909..>3734)
                     /locus_tag="VFR57_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEU0259374.1"
                     /translation="WVIAERADFPADLAKVREFVLKVIGLKVGQSEPLGEKDRARLNL
                     DASGTRVEFLGTDGKLLGAFVVGKKHFKREVENPDKAIPDGRFIVLPDEAGTAYLVAE
                     PLTQATAKTAEWVDRGSFQVEKVKRLEVRYPGGGGWRIERAADNADWRLVGAKPGEKL
                     DIPRANAASYSLQLLELADVASKDATTTVEISGLDKPISIDATTLEGASYAIKVGKVV
                     GDNYYVTFTASGIKPDAKDAERVKIQSQHVLLIPKSKLEDTLKQRADLLEKKEKKK"
BASE COUNT          585 a         1207 c         1247 g          695 t
ORIGIN      
        1 cgccatcggg gtcgccgctg ccggcgcggc tgccgtcgat cagcacgatg ccggcgacgc
       61 gctcgggggc gatgcggttt gcttcgagca cgacgcggca gcccatgctg tggccgatca
      121 agatgaacgg cggtagctcg aggttgttcg cgagggcaat gacatctcct ccaaaatgct
      181 caatggagca ttcgtgcggc cggccggggg tgcttccgtg gccgcgcagg tcgcaggcga
      241 ccacttcatg tttctgcttg agttccttga gctgaagccg ccagtcctcg tggctgcaga
      301 gaaagccgtg cacgaagacc aggggcgggg caccccgccc ctggtgcacg aagtgcaggc
      361 ctattcctcg gcgctggcgg ccggcttgtg cgcggagacg agcttttgct gaagctgcgg
      421 cggcgcagga tcgtagtggc tgaactccag gctgtaggag ccgtggcccg cggtgagcga
      481 cttcaaccgc gccggaaagt gctcgagctc cgagagcggc gcctgcgccg taatctgcac
      541 tagtcccggc cgaggggaat ccgagccctt gatctggccg cgatggccgg agagctcgcc
      601 cgcgatgtcg cccatgcggt cttccggcgc gacgatctcc acgttgacga tcggctcgag
      661 cacgatgggc cgcgccttgg cgagcgcgtc gaggaacgcc ttgcggccgg cggactggaa
      721 ggcaacgtcc ttggaatcga ccggatggct cttgccgtcg tacacgatca cgcgcacgtc
      781 ctgcagcggg tagccggcga ggaaacccga gtcgagcacc gcgcgcacgc ctttctcgac
      841 cgacgggatg aactggttcg ggatcgcgcc gcccttcacg ctgtcgacga actcgaagcc
      901 cttgccgcgc ggcaggggtt ccaccttaag gaacacctcg ccgaactggc ccgcgccgcc
      961 ggtctgcttc ttgtgccggc tatggccctc ggccctggcg gcgatggtct cgcggtacgg
     1021 gatcgacggc gggcgcgtat cgaggtcgag cttgtattgc gaggacattt tctccagcac
     1081 cgccttgagg tgcatctcgc ccaggccgcg catcacggtc tcgttcgcct gcgagttgtg
     1141 ctcgatacgg aagcacgggt cctcggccag gatcttgtgc agcacgtcgg acaccttctg
     1201 ctcgtcgccg cgcttcttcg gctgcaccgc caggccgaac accggcgtcg gcaggtcgag
     1261 cggcttggcg tgcacgtttg cgtcctcggc cgagtcgtgc aggatgtggt cgaactggat
     1321 ttcgtcgatc ttggcaatcg cgcagatgtc tcccgggatc gcctcgtgaa cctcgacctg
     1381 cgtctttccg cgcagcaggt agaggtgccc ggccttgatc ggcttgcggc cttcgccgat
     1441 atagagctgg ctgcccgcgg tgagccggcc ctggtgcacg cggaacaccg cgacgcggcc
     1501 gatgtacgga tcgatctcga tcttgaacac gtgcgctagc gcgtgcttgc ccggatcggg
     1561 caccggctgc aggtcgctgc cctggccatt gcccgctttg gtgacgacgt aggaaggcgg
     1621 gttgccctcg gtcgggttcg gcagcagctt cacgataacg tcgagcagct ccgcgacgcc
     1681 gatgccggtc ttcgcggagg tgaacactac cgggatgaga tggccctcgc gcagcgcgcg
     1741 ctcaagcggc tcgtgcagct gctcggggct cacttcgccc ttctcgaggt agtgctccat
     1801 cagcttctcg tccacctcga ccacctggtc gacgagcttg ccgtgcgctt ctccgaccga
     1861 ggagaagtcg gcttcgcccg agggcgcgaa gaagcagtcc gagaccttcg agcggttgcc
     1921 ggcgggcagg ttgagcggca agcattcctt gccgaacgcc tgctggatgc gctcgagcag
     1981 gccctcgagg tcgacgttgt cggcgtcgat cttgttgacg atgatgaggc ggtcgaggcc
     2041 ccgctttgcc gcccagtgca aaaagcggcc ggtcatcatc tctaggccgt tctgcgcgtt
     2101 gatcacgatc gccgccgttt ccacggccgc gaacgcggac agagcatgcc cgaggaagtc
     2161 gggatagccc ggcgtgtcca gaaggtgaat gcggtggtcc tggaaatcga agctcgcgac
     2221 cgcaagcttc agggaatgct gcagctgctt ctcgagcggg gtgtagtcgc agacggtagt
     2281 gccgcgctct acgctgcccg ccgcggggat cgctcccgct gtggcgagca gcgcttcggc
     2341 cagggtggtc ttgcctgcac ccgactggcc gacgagcgcc atcgtcctta tgcgtttgat
     2401 cgaatgtgcc acgggcctgc ctcctcttct cgaaatttat cttaccgcgg cacatggcgg
     2461 ggggtcgaga ccgccagaaa gatctccctc aggaagagca gcaggcacac gatgagcgcc
     2521 accatcgcgc agacgaacat gccggcgatg gtgaatgaca ggtcggtgcg cacgaacgcg
     2581 ccgacgaagg cacccgcgat cagcagcgcg gtgaaaagcg ctgaaatcac ggcgaaaacg
     2641 atcgcaaggt agacgaagcg gatgcgccgc gcaattgtgg cgagctcgtc ctgcgccgaa
     2701 ggcagctcgg cctgcgacat ggcggcgagc cgctcctcga gctcgcggcg ccggtccacc
     2761 gcccggccga gccgcaggtt gagcgcgttg atcaccgtgc cgagcgccgt cagcatgaac
     2821 accggggcga tcgcgagctg aatcaccccc gcgatctcgg gcagatgcgg gtccaacgcc
     2881 acgccgcgag tctagcgcgg cggcaagcct acttcttttt ttccttcttc tcgagcaggt
     2941 cggcgcgctg cttcagcgtg tcctcgagct tggacttcgg gatgaggagg acatgctgcg
     3001 actggatctt cacgcgctct gcgtccttcg cgtcgggctt gatccccgag gcggtgaacg
     3061 tgacgtagta attgtccccg acgaccttgc cgaccttgat cgcgtaggag gcgccttcga
     3121 gcgtggtcgc gtcgatgctg atcggcttgt cgaggccgct tatttctaca gtcgtggtcg
     3181 cgtccttgga ggcgacgtct gcgagctcca gcagttgcag cgaatacgag gctgcatttg
     3241 ccctcgggat gtcgagcttt tcgccaggct tcgcgccaac aagcctccag tccgcgttgt
     3301 ccgctgcgcg ctcgatccgc catccgccgc ctccggggta gcgcacttcc agtctcttga
     3361 ccttctccac ttggaaagag ccgcggtcga cccattcggc cgtcttggcc gtggcttgcg
     3421 tgagcggctc ggcgaccaga taggcggtgc cggcttcgtc gggcaggacg atgaagcgtc
     3481 cgtccgggat ggccttgtcc gggttttcca cctcgcgctt gaagtgcttc ttgccgacca
     3541 cgaaggcacc gagcagcttg ccgtcagtgc caaggaattc gaccctggtc cccgaagcgt
     3601 cgaggttgag gcgcgcgcgg tccttctcgc cgagcggctc gctctggccg accttcaggc
     3661 cgatcacttt gagcacgaac tcgcgcacct tcgcgagatc cgcagggaaa tcggcgcgct
     3721 cggcgatcac ccag
//
LOCUS       DASTPD010000247         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1558723, whole genome shotgun sequence.
ACCESSION   DASTPD010000247 DASTPD010000000
VERSION     DASTPD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558723"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFR57_08165"
     CDS             complement(<1..1252)
                     /locus_tag="VFR57_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0259375.1"
                     /translation="MNFAPQKVEMQRRADGTILLRSPQKLGSYARCVTEWLVRWSDKA
                     PDRTFLAERVGERWKKLNYRETYGAVRRIAQALLDRGLGPERPVAILSDNGLDHALLA
                     LGAMHVGIPVAPVSPAYSLMSQDFGKLKYIFELVQPGLVFAADAQKFAPALAAVGAKS
                     TSVAELLETNPGSTQEREHAKVRPESVAKILFTSGSTGMPKGVVNTHRMLCANQQMLA
                     QAWPLVEARPPVVVDWLPWNHTFGGNHNFNLVLRNGGTMYVDAGKPVPGLVDITARNL
                     KEVAPTMYFNVPRGYDLLLPLLEKDAELRCNFFRDLEVLFYAAAALPQNLWDKLQKVA
                     KAEGRSPAMLSAWGSTETAPLATSVHFPMERAGVIGLPVAECELKLVPAAGKLEVRVR
                     GPNVTPGYHKRPDLTKLAFDEEGFY"
     gene            complement(1249..2196)
                     /locus_tag="VFR57_08170"
     CDS             complement(1249..2196)
                     /locus_tag="VFR57_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259376.1"
                     /translation="MRFILLALLVASSQLGQAQQYPAKPLRMLVGYSAGGGADALARL
                     IAVKIADGLGQQVLVENRPGAGATIAAAAVASSPADGYTIFFADTALLIAPAIYQKLS
                     FDPVKSFAPVSGACTTPLVIAVNNEVKAASVAELISALKANPGRLSYGSPGIGTVHHL
                     AMETFKRQAGVDIVHVPYKGASAIIPDVISGQLQIGIISAPPALAQSKAGRLRPIALT
                     SPVRVAGADWPALADTLPGFDASPRLFVLAPAGTPAGVVTKLNETLKTALASKDLLDA
                     FSAQGAFADWSTPQALGVVMAQESLRWAQVARDAGIKAE"
     gene            2247..3101
                     /locus_tag="VFR57_08175"
     CDS             2247..3101
                     /locus_tag="VFR57_08175"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU0259377.1"
                     /translation="MEEQLRFMDQITSTAIDLSFRFGPKLLVAVLVLIAGVVAGRWAA
                     RGAERALARFELEPPVVALIRRVTYVVVFGLFVIMALQNLGVELLPLIAGLGIAGAGI
                     ALAMQGVLGNAVAGLTIIFSRPFRVGDYISIANEEGEVLEIQLFSTTLGHVDRSRVVI
                     PNRKIAGEILHNYSRIRQLAIDVMVTNDTDIATALAVAVEATRGNARVLKEPAPFAAV
                     VKLEAGGVVLGARAWTSVSDYAPAGGEVRQAILGTFRQRGISAPVPQREIRMVGTDAY
                     IGQRRDAA"
     gene            complement(3098..3466)
                     /locus_tag="VFR57_08180"
     CDS             complement(3098..3466)
                     /locus_tag="VFR57_08180"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEU0259378.1"
                     /translation="MLEAERAGAKALVVFMDDWPRNGPEWKVLRRIHDDEAHNCALIG
                     KLLEKTGTPYSHATGKFYEKAVAVKGAKERIAFLVRGLKWAVKRFEAALPTLDREAQD
                     VFTRMRDSHLRSIAACERLI"
     gene            complement(3480..4892)
                     /gene="boxB"
                     /locus_tag="VFR57_08185"
     CDS             complement(3480..4892)
                     /gene="boxB"
                     /locus_tag="VFR57_08185"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEU0259379.1"
                     /translation="MSSINYAEKIPNNVNLVSDRTLQRALEHWQPHFLEWWKTMGPSD
                     FQAAAVYLRTAISVDAQGWAHYGAVKMPDYRWGIFLADPLPGRTIGFGDQMGEPAWQQ
                     VPGEYRSTLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHAYFGRDGREEAEELLARHSGDADKPRILTTFNEPISDWLSFYCFTYFTDRDGKY
                     QLKSLAESAFDPLSRTCRFMLTEEAHHMFVGETGIGRVVKRTLEVMKELGTEDPARVR
                     AAGAVDLPTLQKYLNFWCSSSLDLFGSEISSNSAANFAHGLKGRPDESQYEDHVLREK
                     SMALLTPEGEQQVPMLNALNEVMRESYLKDCEIGLKRWNRQIERAGFPFRLTLPSSRF
                     RRSIGAWAGQPVDPQGKMLSKEEYEKRLPDWIPSEADRTFVHSLQQKVVAPGKMAAWI
                     APPERGINNLPVEYEYVKLQ"
     gene            complement(4954..6522)
                     /gene="boxC"
                     /locus_tag="VFR57_08190"
     CDS             complement(4954..6522)
                     /gene="boxC"
                     /locus_tag="VFR57_08190"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEU0259380.1"
                     /translation="MISFETQPDRYNHWKLAFDGPVATLNLDVAEDKPLEPGYKLKLN
                     SYDLGVDIELADALNRIRFEHPEVKTVVITSGKSRMFCSGANIYMLGHSSHASKVNFC
                     KFTNETRNGLEDSSAHSGLKFIAALNGTTAGGGYELALACDEIAMIDDRSSTVSLPEV
                     PLLGVLPGTGGLTRLVDKRKVRRDLADVFCTTAEGVRADRAKQWRLVDHIAKPQQFAE
                     MVKARALELGNKSDRPGGEGVALTPLGPNRLVEVDLDRGARIATLTVKGPEAVKVEKS
                     ADWYPLRLARELDRAILDLRHNHLDIGLWVLKSKGDVKKMLELDAALQKSKDDWFARE
                     TIGLLRRTLSRLDISSRSIFAIIEKDSCFAGTLLELVLAADRSYMLDVDQGPSVALSE
                     MNFGAYPMANGLSRLQTHYCGELPDLAKEKILSTTEARDLGLVTSTPDDIDWDDEVRI
                     AIEERASLSPDALTGMEASLRFAGPETMLTRVFGRLTAWQNWIFVRPNAVGEQGALKV
                     YGTGAKAKFNWERV"
BASE COUNT         1075 a         2110 c         2231 g         1207 t
ORIGIN      
        1 ggtaaaagcc ttcctcgtcg aaagcgagct tcgtgaggtc ggggcgcttg tggtagccgg
       61 gcgtgacatt cggtccccgc acgcgcactt cgagcttgcc cgccgccggt acgagcttga
      121 gctcgcactc cgcaaccggc aggccgatca cccccgcgcg ctccatcgga aaatggacgg
      181 aagtggcgag cggcgcggtt tcggtcgagc cccaggccga aagcatggcc ggcgagcggc
      241 cttcggcctt ggcgactttc tgcagcttgt cccagaggtt ttgcggcagc gccgccgcgg
      301 cgtaaaagag aacttcgagg tcgcggaaga aattgcagcg cagctcggcg tctttctcga
      361 ggagcggcag cagcaggtcg tagccgcgcg gcacgttgaa atacatggtc ggcgcgactt
      421 ctttcagatt gcgcgccgtg atgtccacga ggcccgggac gggcttgccc gcgtcgacgt
      481 acatcgtgcc gccgtttcgc agcacaaggt tgaagttgtg attgccgccg aaggtgtgat
      541 tccagggcag ccagtcgacc accacgggcg ggcgcgcttc gacgagcggc caggcctgcg
      601 cgagcatctg ctggttcgcg cacagcatgc ggtgcgtatt gaccacgcct ttcggcatgc
      661 cggtcgagcc cgaagtgaag aggattttgg cgacgctctc gggccgcacc ttcgcatgct
      721 ctcgctcctg cgtggagccg ggattggtct ctaggagctc ggcgaccgat gtcgatttcg
      781 cgcctaccgc cgccagcgcc ggcgcgaact tctgcgcgtc ggcggcgaac acgaggccgg
      841 gctgcacgag ctcgaagatg tacttcagtt tcccgaaatc ctgcgacatg agcgagtagg
      901 cgggcgacac tggcgccacc ggaataccca cgtgcatggc gccgagggcg agcagcgcat
      961 ggtcgagacc gttgtccgac aggatcgcca ccggtcgctc cgggcctagg ccccgatcga
     1021 gcagcgcttg ggcgatgcgg cgcaccgcgc cgtaagtctc gcgatagttg agctttttcc
     1081 agcgttcacc gacgcgttcg gcaaggaagg ttcggtccgg cgccttgtcg gaccatcgca
     1141 cgagccattc ggtgacgcag cgcgcgtagg agccaagctt ctggggcgaa cgcaggagta
     1201 tcgtgccgtc cgcacgcctt tgcatctcga ccttctgcgg cgcgaagttc attctgcctt
     1261 gatgcccgcg tctctcgcga cctgcgccca gcgcagcgac tcctgcgcca tgacgacgcc
     1321 gagcgcctgc ggcgtggacc agtcggcgaa cgcgccttgg gcgctgaagg cgtcgaggag
     1381 atccttggaa gcgagcgcgg ttttcagcgt ctcgttgagc ttcgtgacca cgccggctgg
     1441 cgtgccggcg ggtgcgagca cgaaaaggcg cggtgacgcg tcgaagccgg gcagcgtatc
     1501 ggcgagcgct ggccagtcgg cgccggccac ccggaccggg ctcgtcagcg cgatcggacg
     1561 caggcgtcca gccttcgact gcgccagcgc gggcggcgcg ctgatgatgc cgatttgcag
     1621 ctgtccggag atcacgtccg gaatgatcgc ggaggcgccc ttgtacggga catgcacgat
     1681 gtcgacgccg gcctgacgct tgaacgtttc catggcgagg tgatgcacgg taccgatgcc
     1741 gggcgagccg tagctgagac ggccggggtt ggctttcagc gcggagatca gctcggcgac
     1801 gctcgccgcc ttcacctcgt tattgaccgc gatgacgagc ggggtggtgc acgcgcccga
     1861 gaccggcgcg aagctcttca ccgggtcgaa gctaagcttc tggtagatcg ccggcgcaat
     1921 gaggagcgcc gtgtcggcga agaagatggt gtagccgtcc gccggcgagc tggcgaccgc
     1981 agcggccgcg atggtcgcgc ccgcgcccgg ccgattctcc accagcacct gctggccgag
     2041 gccgtcggcg atcttcacgg cgatcaggcg cgcgagcgcg tcggcgcctc cgcccgcgct
     2101 gtagccaacg agcatgcgca gcggcttcgc gggatattgc tgtgcctggc cgagttggga
     2161 cgacgccacg agcagcgcga gcaggatgaa gcgcatctga gcgctgtatc attgcgcatc
     2221 accacgtgtc aataacggga ggggtcatgg aagagcagct gcgctttatg gatcaaatca
     2281 cgagcacggc gatcgatctc tctttccgat tcggcccgaa gctgctggtc gctgtcctcg
     2341 tgctcatcgc cggcgtcgtg gcgggacgct gggccgcgcg gggcgctgag cgtgcgctcg
     2401 cccgcttcga gctcgagcct ccggtcgtcg cgctgattcg gcgcgtgacc tacgtcgttg
     2461 tgttcggcct tttcgtcatc atggcgctgc agaacctcgg tgttgagctc ttgccgctca
     2521 tcgccggact gggtatcgct ggcgccggta tcgccctcgc catgcaagga gtgctcggca
     2581 atgcggtcgc cggcctgacg atcatctttt cgcgcccctt tcgcgtcggc gactacatct
     2641 cgatcgcgaa cgaagaaggc gaggttctgg agatccagct cttcagcact acgctcggcc
     2701 acgtggaccg ctcgcgcgtc gtgatcccga accgcaagat cgccggcgag atcctgcata
     2761 actacagccg catccgccag cttgccatcg acgtcatggt gacgaacgac acggacatcg
     2821 ccacggcgct cgcagttgcc gtggaggcga cgcgcggcaa cgcgcgcgtg ctgaaggagc
     2881 ccgcgccgtt cgcggctgtg gtgaagctcg aggcgggcgg cgtggtgctc ggcgcgcgcg
     2941 cctggacctc cgtgtccgac tacgccccgg caggaggcga ggtgcggcag gcgattctcg
     3001 gaaccttccg ccagcgcggt atctcggcgc cggtacccca gcgcgagatc cgcatggtcg
     3061 gcaccgacgc ctacatcgga cagcgccgcg acgccgccta gatcagccgc tcgcaggcgg
     3121 cgatgctgcg caggtgcgaa tcccgcatcc gggtgaagac gtcctgcgcc tcgcggtcga
     3181 gcgtggggag cgcagcctcg aatcgcttga ccgcccattt cagcccgcgc acaaggaacg
     3241 cgatgcgttc cttggcccct ttcaccgcga ccgctttctc gtagaacttg ccggtggcgt
     3301 ggctgtaagg cgtcccggtt ttctcaagaa gcttgccgat gagcgcgcag ttgtgcgctt
     3361 cgtcgtcgtg aatcctgcgc agcaccttcc actcggggcc gttgcgcggc cagtcgtcca
     3421 tgaagacgac gagcgctttc gcgcccgcgc gctcggcctc gagcatttcg ttgagaaagc
     3481 tactggagtt tgacgtactc gtactccacg ggcaggttgt tgattccgcg ctcaggcggc
     3541 gcgatccacg ccgccatctt gccgggtgcg acgaccttct gctggagcga gtgcacgaat
     3601 gtcctgtctg cctcggacgg aatccagtcc ggcaggcgct tttcgtattc ctccttcgaa
     3661 agcatctttc cctgcgggtc caccggctgg cccgcccatg ccccaatgga gcggcggaag
     3721 cgcgaggagg gcagcgtaag gcggaaaggg aagccggcgc gctcgatctg gcggttccag
     3781 cgcttgaggc cgatctcgca gtccttgagg taagactcgc gcatcacctc gttcagcgcg
     3841 ttcagcatcg gcacctgctg ctcgccttcc ggagtcagca gcgccatgct tttctccctc
     3901 aagacatggt cctcgtactg cgactcgtcg gggcggcctt taaggccgtg cgcgaagttc
     3961 gcggccgaat tggacgagat ctccgagccg aacaaatcga gcgagcttga gcaccagaag
     4021 ttcagatact tctgcagcgt gggcaggtcg accgcgccag cggcgcgaac cctggccggg
     4081 tcctcagtgc cgagctcctt catcacctca agcgtgcgct tcaccacgcg gccgatgccc
     4141 gtctcgccga cgaacatgtg gtgcgcctcc tcggtgagca tgaagcggca ggtgcgcgag
     4201 agcggatcga acgcgctctc ggcgaggctc ttcagctgat acttcccgtc gcggtcggtg
     4261 aaataggtaa agcagtagaa cgacagccag tcggagatcg gctcgttgaa ggtggtgagg
     4321 atgcgcggct tgtcggcgtc gcccgagtgg cgagcgagca gctcttccgc ttcctcgcgg
     4381 ccgtcgcgcc cgaagtaggc gtgcagcagg tacaccatcg cccagaggtg gcggccctcc
     4441 tcaacgttga cctggaacag gttgcgcagg tcgtacagcg acggacaggt gtggccgagg
     4501 agccgctgct gctcgaccga ggcgggctcg gtgtcacctt gcgtcacgat cagccgccgc
     4561 agcgtcgagc ggtactcgcc cggcacctgc tgccacgccg gctcgcccat ctggtcgccg
     4621 aagccgatgg tgcgacccgg tagcggatcg gcgaggaaga tgccccagcg gtagtcgggc
     4681 atcttcaccg cgccgtaatg cgcccagccc tgcgcgtcca ccgagatcgc ggtgcgcaga
     4741 taaacagcgg ccgcctggaa gtcgctcggc cccatcgtct tccaccactc gaggaagtgc
     4801 ggctgccagt gctcgagcgc gcgctgcagc gtgcggtcgc tcacgaggtt gacgttgttc
     4861 ggaatcttct ctgcgtaatt gatgctgctc atcgcacacc tctgaaaaag gggtcaggac
     4921 ccttttcgtg gaaaaagggt cggatacctc gcgctaaacg cgttcccagt tgaatttcgc
     4981 cttggcgcca gtcccataaa ccttgagcgc gccttgctcg ccgacggcgt tgggtcggac
     5041 aaagatccag ttctgccatg cggtgaggcg tccaaacacg cgtgtgagca tcgtctccgg
     5101 cccggcgaag cgcaggctcg cttccatgcc ggtgagcgcg tcgggcgaga ggctcgcgcg
     5161 ctcctcgatg gcgatgcgca cttcgtcgtc ccagtcgatg tcgtccggcg tcgaagtgac
     5221 aaggccgaga tctcgcgctt ctgtggtcga aaggatcttc tccttggcga gatcgggcag
     5281 ctcgccgcag tagtgcgtct gcagtcgcga cagcccgttc gccatcggat acgcgccgaa
     5341 gttcatctcc gacagcgcaa cgctcggacc ctggtccacg tccagcatgt agctccgatc
     5401 ggcggcgagc acaagctcga gcagggtgcc ggcgaagcag gagtctttct cgatgatggc
     5461 aaagatcgag cgcgaggata tgtccaggcg cgaaagcgtg cggcgcagca aaccgatggt
     5521 ctcgcgcgcg aaccagtcgt ccttgctctt ctggagcgca gcgtcgagct ccagcatctt
     5581 cttcacgtcg cccttcgact tgagcaccca caggccaatg tcgaggtggt tgtggcgcag
     5641 atcgagaatg gcgcggtcga gttcgcgcgc aagccgaagc gggtaccagt ccgcgctttt
     5701 ttccaccttc actgcctccg gacccttgac ggtcagcgtg gcgatgcgcg cccccctgtc
     5761 gagatccacc tcgacgaggc ggttcggtcc gagcggagtg agcgccacac cttcgccgcc
     5821 ggggcgatcg gacttgttgc ccagctcgag cgcgcgcgct ttcaccatct cggcgaattg
     5881 ctgcggtttg gcgatgtggt cgacgaggcg ccactgcttc gcccgatccg cgcgcacgcc
     5941 ttcggcggtg gtgcagaaaa catccgcgag atcgcggcgc actttgcgct tgtcgacgag
     6001 gcgggtcagg ccgccggtgc cgggcagcac tcccagaagc ggaacttccg gaaggctcac
     6061 ggtggacgag cggtcgtcga tcatcgcgat ctcgtcgcaa gcaagcgcga gctcgtagcc
     6121 gccaccggcg gtggtgccgt tcagcgcggc gatgaacttg agtcccgagt gggccgaaga
     6181 atcttcgagg ccgttgcgcg tctcgttggt gaacttgcag aagttcacct tggaggcgtg
     6241 gctcgagtgg ccgagcatgt agatgttggc gccggagcaa aacatgcggc tcttgccgct
     6301 cgtgatgacg acggttttga cctcgggatg ctcgaagcgg atgcggttca gcgcgtcggc
     6361 gagctcgatg tcgacgccga ggtcgtagct gttcagcttg agcttgtatc ctggctcgag
     6421 cggcttgtcc tcggccacgt cgaggttgag cgtggcgacc ggtccgtcaa aggcgagctt
     6481 ccagtggttg tagcgatcgg gttgggtctc gaaagagatc atgtctttac cagagagagg
     6541 agctgggaga ggctcgcgcg cagcgtcttg ccggaagtat cgagcgtgac atcggcgcgg
     6601 ctgtagaggc ggtcgcgatc ggc
//
LOCUS       DASTPD010000248        14239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_538371, whole genome shotgun sequence.
ACCESSION   DASTPD010000248 DASTPD010000000
VERSION     DASTPD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538371"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFR57_08195"
     CDS             <1..399
                     /locus_tag="VFR57_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0259381.1"
                     /translation="AVVSGVPVTDMLRFDRFTGGRAWVPEYGSPRDPQQFQNLVRYSP
                     LHNVKPGTCYPATLVTTAEFDDRVVPNHAYKFAAAVQAAQPCPRPVLLRYEHKASHGY
                     TPTQRWIAELADVWAFAAEHTGMRRPALAH"
     gene            complement(422..772)
                     /locus_tag="VFR57_08200"
     CDS             complement(422..772)
                     /locus_tag="VFR57_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259382.1"
                     /translation="MKVKRIVSNVASADIEKATAFYRDILGLKVLMDLGWIRTYGSNA
                     KMTIQVSVMSEGGSGTAVPEMSIEVDDLDDALTRVREAGIAIEYGPASEPWGVRRFFV
                     RDPFGKLINILQHE"
     gene            complement(802..1674)
                     /locus_tag="VFR57_08205"
     CDS             complement(802..1674)
                     /locus_tag="VFR57_08205"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU0259383.1"
                     /translation="MSFPASATEQGVQARISELLVAGSLEISPRELHRAGEVASQLPA
                     NTCVYIPSLPGLPLSRTLEAISALRAAGLDPVPHVSARRILNEAEFRSFLKEAVARHG
                     VHRVLLLGGDEDKPKGPFSDSLQILRSGMLRDSGIREIGVAGYPEGHPRISATALIEA
                     FEEKRRLARDQGLGIYVVTQFSFAPHRVVEYCAGLARTASEVSVYAGVAGPTDPAALA
                     RYAQRCGVSVSLRALKTLGTGIARLVTNSDPKDQLVALARYSLQREQSNLVGVHLYSF
                     GGAAKTAAWIREHI"
     gene            complement(1649..3052)
                     /locus_tag="VFR57_08210"
     CDS             complement(1649..3052)
                     /locus_tag="VFR57_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259384.1"
                     /translation="MDIAAGGSLLGSISEFCRRTGMAESTFGRRAVNDGKFVARLRDG
                     ARVTPETLDRVNQFMVKHGAPGAGDAPRELLPLIRPGAVQAVALQSRDAPPEKNFRFF
                     DNRQKYLLFVNTCSEKDVVAKRVGLELGHIHPRPPSVRLFDAGMGDGTVLARVMREMH
                     RRFPTVPFYIVGKEISLEDVRLSLRKMPDRFYEHPATVLVVTNMYYTEAPWLTPRTVP
                     AATSMVWHEVALTGATSHDFSEQITALEPFLAKNWQARHSPKTGNPIYDHPVALVLYR
                     DDYRFMLDEVIPERGQGKADYDMVIASQPYRLRAPLEFKAEKVVAPLVRALGKGGRLL
                     GIHSHGDDPGLEIIQKVWPGENPYTQTRHDILRATKSQLGKEARHYNFNAYADARALF
                     RYEMHTLPTEISASIGTSTLFAAWNAAIYVAQMDDVRLSEVLRDTRYLDATREVLQKH
                     GSLWFLDESYVVSRKRD"
     gene            3131..4486
                     /locus_tag="VFR57_08215"
     CDS             3131..4486
                     /locus_tag="VFR57_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0259385.1"
                     /translation="MNQILELIGETPLVEVRRLAVGRCRLFLKLENQNPGGSIKDRVA
                     LAMIEAAERDGKVQPGSLIVEATAGNTGLGLALVAAQKGYRLLIVVPDKMSQEKIFHL
                     KAMGAEVQLTRSDVTKGHPEYYQDMAARLAREHGAFYVNQFANEANPRAHEEGTGPEI
                     WEQMEHKVDAVVAGVGTGGTLTGLSRYFARISPQTEMVLADPVGSILVEYVRTGKVGK
                     AGSWLVEGIGEDFIPSVADLSRVKKAYAISDGEAFLTCRELLKKEGIMAGTSSGTLIA
                     AALRYCREQSEEKRVVSFVADSGNKYLSKVYNDYWMIDQGFIQREQYGDLRDLIARRH
                     REHAVATVNADETVLAAHQRMKLYDISQLPVLKDGKIVGIVDEEDILLEVYDNPAHFR
                     EPVTEAMESRLVTVPPSAPIEQLMEIFNRGLVAIVVDGDEFFGLITRIDFLNWLRRRA
                     Q"
     gene            4483..5640
                     /locus_tag="VFR57_08220"
     CDS             4483..5640
                     /locus_tag="VFR57_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0259386.1"
                     /translation="MKFETKVIHAGQEADPRTGAVMTPIYATSTYAQSSPGEHQGFDY
                     ARTRNPTRDALEACIAELESGKAGFAFASGMAAIGTVLELLDSGSHIVAMNDLYGGSY
                     RILERVRKRSANLAASFVDLTSLQAFENAIRPETRMVWVETPTNPLLKIVDLEAVAAI
                     ARKRRLISVCDNTFASPWIQRPLELGFDIVVHSTTKYLNGHSDVIGGAAVLKEKGELA
                     DRLSFLQNAVGGVPSPFDAFLTLRGIKTLALRMERHCGNAMHIAAWLEKHPKVARVIY
                     PGLATHPQHKLAARQMNARYGGMVTAVLKGGKEGALSASKRFLERCKLFTLAESLGGV
                     ESLIEHPAIMTHASLPEDVRAGLGIDDGLVRLSVGIESVDDLLAELKYALE"
     gene            5693..6556
                     /locus_tag="VFR57_08225"
     CDS             5693..6556
                     /locus_tag="VFR57_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0259387.1"
                     /translation="MLRMPGKSYAGPLPPLSGEARALSLRLRLHVAALAAGERNADLE
                     RPARYIEQAFAAQGYKTQAQTFRSGGRTVRNIEAGAGPIIVGAHYDTVPGSPGADDNA
                     SGIAVLLELAAMKLPVRFVAFANEEAPYFLGEEMGSFVYARERAAGVRAMFSLEMLGY
                     YRDHPGSQRYPAPLGFFYPDRANFIAFVADLGARRLLHRSIEAFRKHAQFPSEGVAAP
                     SFIPGVTWSDHWSFRQHGYPAIMITDTAFYRYPHYHLPSDTPDKLDYERMARVALGLA
                     AMLKELANESR"
     gene            6543..7358
                     /locus_tag="VFR57_08230"
     CDS             6543..7358
                     /locus_tag="VFR57_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0259388.1"
                     /translation="MRVGDLEVNRLGFGAMRVLGTPDIWGPPKDPANARKVLRRAYEL
                     GANFFDTAESYGPHTNETVIAEAMRPYPKDLVIATKCGLVRPKPSRWDEDARPEKLRG
                     DLEGSLKRLKLDCLHLWQLHAPDPRVPLEESLGVMADAQRAGKVRHIGVSNFSVKELA
                     RARKVAKIVSVQNRYSLDDRNEEDVLAHCEKLGIAFLPWYPLGAGGAVRASKIKRVAK
                     RLGAAPAQVAIAWLLAKSPVMLPIPGTSSLAHLEENMAAAKIQLSREDLAELD"
     gene            7527..7733
                     /locus_tag="VFR57_08235"
     CDS             7527..7733
                     /locus_tag="VFR57_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259389.1"
                     /translation="MLHLFLAINVGLIVASVLSWTGEPSALVRRDYGVLSWVITGFGA
                     TAAFWSVRDFVAWRSLRRASVAAT"
     gene            7730..8680
                     /locus_tag="VFR57_08240"
     CDS             7730..8680
                     /locus_tag="VFR57_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259390.1"
                     /translation="MKTILLALMLVTGAAAAQDQYPSKPIRILVGYAAGGGNDIIVRV
                     LQPEMQKGLGQPVIVENKPGAQSIVAAELAARAAPDGYTIFMGPSGPMTINPATYAKL
                     PYHPQRDFAPISMICEFPLIVTVDAKLPVKSVKELIDYAKANPGKSNYASSAGIFQIT
                     TELFKQKTGTSIEMIPYKSSGESVQAVVGGTVMMTIVDPPPATGPLKAGALRGLAVTS
                     GKRHPAWPDIPTMAEAGVPDMEVPVWTAFFAPAKTPPAIVARLQKEVARAVQTPEVRE
                     RFAQMALEPVGGSSEDLGKRVARDIEKWTAVAKAANIKND"
     gene            complement(8705..9676)
                     /locus_tag="VFR57_08245"
     CDS             complement(8705..9676)
                     /locus_tag="VFR57_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0259391.1"
                     /translation="MMRLPWFQYRAPQSVAEAARILAGEGDAMLIAGGTDLLPNMKRR
                     QMAPKMLVSLSSIDALKRNGSVYGAGLTLTQVVNSNAPKALRQAAHQVATVHLRNMGT
                     LGGNLCLDTRCNYYNQNYEWRKAIDFCLKKDGDVCWVATASKRCVAASSTDCAPALLA
                     LGARVHLVSSSGEREIGLEELYNNDGMDYLKRRPDEILTEVLIPPSSASTYWKLRRRG
                     AFDFPVLGVAAAASPQGVRIALGAVASRPFLVHKANDFLEGKELTDEVIDEAAKLVAS
                     RAKPMDNADLDLYWRKDVAVELARYALRELRGDDMRETRLRIARQLV"
     gene            complement(9673..12099)
                     /locus_tag="VFR57_08250"
     CDS             complement(9673..12099)
                     /locus_tag="VFR57_08250"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0259392.1"
                     /translation="MKVLGKPRRRVDGRAKVTGQTRFADDIALPRMLHMKLLRSPQPH
                     ALIESIDIARAKAHPGVHLVLTGQDFPVTFGILPVSQDEYPLAPQHVRYVGDPVAAVV
                     ARDEQTAAEACDLMEVKYRPLQTISDPVEALQNPEPRIHAYSELGNIHRLQAFEFGDV
                     DEALERSDHVFEDLFFYEGNTHLPIEQHASVAAVDGEGKLTLWSSTQVPHYVHRSLAR
                     VLQLPASHIRVIATPNGGGFGGKCDVCNHEMVVGKAAILLGRPVKICLNREEVFYMHR
                     GRHPVLMKFRTGVTKDGKLTGMHLQTLIDGGGYGSYGTASTFYTGALQTVTYEVPRYK
                     FEAARVFTNKPPCGPKRGHGTPQPRFGQEVQLDKIAEKLAIDPAELRLNMVAKPNSLT
                     ANWLKIGSIGLEQCIRTVVERSAWKSRFRKLGPGRGLGLACGSYLCGAGLPIYFNKMP
                     QSGVQLLVDRSGQVTAFCGATEIGQGSDDVLAAIIAEVLGIDPFDIRCVTGDTGITPV
                     DLGSYSSRVTVMMGNAAIDASNKVKNLIAQATAEQLEVAPDKLVFANGRVAFDGRSVT
                     FAEAVSFAEEKFGTLGALGSYKPPRAPGRYKGAGVGPSPAYSYTACVVEAEVDPATGW
                     ITVPKIWIAHDIGRTINPVLARGQVEGSVYMGLSEALMEEQVFRRLPPKLSRALVHKI
                     PSLLEYKSLTSLDMPEVDTVLIEDPDPNCPFGAKEVGQGPLLPVMPAVANAIYDAVGV
                     RIDELPITPDKVLRALELKAAGKTPRVGPTSFPAVPYPEPVIVPPPWEGGDGNAVKGH
                     ERTKLKVSAA"
     gene            complement(12083..12568)
                     /locus_tag="VFR57_08255"
     CDS             complement(12083..12568)
                     /locus_tag="VFR57_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0259393.1"
                     /translation="MRQVELSLTVNGEPAAVFFAPYKTLLEVLREDLNLTGTKHGCEL
                     GECGACAVLVDGEPKLACLVLALDCQGRRVDTVEGLADGPKLHPLQAAFADLGAAQCG
                     YCTPGILMTAKALLEKEPEPSRERIKEAISGNLCRCTGYQQIFEAIEDAAKRMRDEGS
                     R"
     gene            12696..13349
                     /locus_tag="VFR57_08260"
     CDS             12696..13349
                     /locus_tag="VFR57_08260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0259394.1"
                     /translation="MALRIGDTAPNFQAQTTQGPIDFHQWIGESWAILFSHPKDFTPV
                     CTTELGYMARIKPEFDRRNAKIIGLSADPVEDHAKWAKDIEETQGAAVNYPMIGDPDL
                     KIAKLYDMLPAEATPGKRTPADNATVRTVFMVGPDKKIKLMLFYPMTTGRNFDEILRA
                     LDSMQLTAKHKVATPAQWKQGQDVIIAGSVSDEDAKKLFPQGWKAPKPYLRITQQPK"
     gene            complement(13481..>14239)
                     /locus_tag="VFR57_08265"
     CDS             complement(13481..>14239)
                     /locus_tag="VFR57_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0259395.1"
                     /translation="VIAIQNARLFREIQEKSAELEVANKHKSDFLANMSHELRTPLNA
                     IIGFSEALMDRMFGDLNEKQLEYQKDIHESGKHLLSLINDILDLSKIEAGRMELELSN
                     FHLPTAVSNAVILVRERAMRHGIELVMDIDQRLGEFRADERKVKQILLNLLSNAVKFT
                     PDGGRVDVSAKLDTDKVEIAVKDTGVGIAPEDQASLFEEFKQLGKDSSRKAEGTGLGL
                     ALTKRLVELHGGQVLVDSAVGKGSTFRVLLPARV"
BASE COUNT         2495 a         4613 c         4604 g         2527 t
ORIGIN      
        1 gcggtcgtat ccggcgtgcc ggtcaccgac atgctgcgct tcgaccgctt caccggcggg
       61 cgcgcctggg tccccgaata cggctcgccg cgcgatcctc agcaattcca gaacctggtg
      121 cgttactcgc cgcttcacaa cgtcaagccc ggtacctgct atcccgcgac actcgtgacg
      181 acggccgaat tcgacgaccg cgtcgtgccg aaccacgcct ataagttcgc cgccgcggtt
      241 caggccgcgc agccctgccc tcgtccggtg ctgctgcgct acgagcacaa ggcgtcgcac
      301 ggctacacgc cgacccagcg ctggatcgca gagctcgccg acgtctgggc cttcgccgcc
      361 gagcacaccg gcatgcggcg gcccgcgcta gcgcactagc ttcggcaagg attgtccggc
      421 gttactcgtg ctgcagaatg ttgattaatt ttccgaatgg atcgcgcaca aaaaatctac
      481 gcactcccca cggctcactc gccgggccgt attcgatcgc gattcccgcc tcgcgtaccc
      541 tcgtcagagc gtcgtcaaga tcgtcgactt caatcgacat ctccgggaca gccgtgccag
      601 aaccgccttc tgacattacg ctaacctgaa tcgtcatctt tgcgttcgaa ccgtaggtcc
      661 gtatccagcc aagatccata agcaccttga gcccgagaat gtccctatag aaagcggttg
      721 ccttctctat gtccgcactc gcgacatttg agacgatgcg cttgaccttc accgatgcga
      781 ccgccgagtc agcgggtcaa ctcagatgtg ctcgcggatc caggcggcgg ttttggcggc
      841 gccgccgaag ctgtagaggt gcacccccac caggttgctc tgctcgcgct gcaggctgta
      901 gcgcgcgagt gcgacgagct ggtccttcgg atcggagttg gtgacgagcc gcgcgatacc
      961 ggtgccgagc gtcttcagtg cgcgcagcga gacgctgacg ccgcagcgct gcgcgtagcg
     1021 cgccagcgcg gccgggtcgg tggggccagc gacgccggcg tagaccgaaa cttcggaagc
     1081 ggtgcgcgcg aggccggcgc agtattcgac cacgcgatgc ggcgcgaagg agaattgtgt
     1141 aacgacgtag atgccgaggc cctggtcgcg cgcgaggcgg cgcttttctt cgaacgcctc
     1201 gatgagcgcg gtggctgaga tgcgcggatg gccttccggg tatccggcga cgccgatctc
     1261 gcggatgccg gagtcccgaa gcattcccga gcgcaggatc tgcaggctgt cgctaaaggg
     1321 acctttcggc ttgtcttcgt cgccgccaag caggagcact cggtgaaccc catggcgcgc
     1381 gaccgcctct ttgaggaagg agcggaactc cgcttcgtta aggatgcggc gggcggagac
     1441 gtgcggcacg ggatcgaggc cggcggcgcg caacgccgag atcgcttcga gcgtgcgcga
     1501 aagcggcaag ccgggaagcg acgggatata cacgcacgta ttcgccggta gctggctcgc
     1561 aacttcgccc gcgcggtgga gctcgcgcgg cgaaatctcg agcgagcccg cgacgagcag
     1621 ttcggagatg cgcgcctgca cgccctgctc agtcgcgctt gcgggaaacg acataggact
     1681 cgtcgagaaa ccagaggctg ccatgttttt gcaagacctc gcgcgtggcg tccaggtagc
     1741 gggtgtcgcg caggacttcg gagagccgca cgtcgtccat ctgcgcgaca tagatggcgg
     1801 cgttccacgc ggcgaacagc gtcgaggtgc cgatgctcgc cgagatctcg gtgggcagcg
     1861 tgtgcatctc gtagcggaaa agggcgcgcg cgtcggcgta ggcgttgaag ttgtagtgcc
     1921 gcgcctcctt gccgagctgg ctcttggtgg ctcgcaggat gtcgtggcgc gtctgcgtgt
     1981 acgggttctc acccggccag actttctgaa tgatctccag ccccgggtcg tcgccgtgcg
     2041 agtggatgcc gagcagcctg ccgcccttgc caagcgcgcg caccagcggc gcgaccacct
     2101 tctcggcctt gaactcgagc ggcgcgcgca ggcgataggg ctgcgaagcg atcaccatgt
     2161 cgtaatcggc cttgccctgc ccgcgctccg ggattacctc gtccagcatg aagcgatagt
     2221 cgtcgcggta gaggacgagc gctaccgggt ggtcgtagat cggattgccc gtcttcgggc
     2281 tgtggcgcgc ctgccagttc ttcgcgagga agggctcgag cgcggtgatc tgttcggaaa
     2341 agtcgtgcga cgtagcgccg gtgagcgcga cttcgtgcca gaccatggag gtcgccgccg
     2401 gcaccgtgcg cggcgtaagc cacggcgctt ccgtgtaata catgttcgtg acgacgagca
     2461 cggtggccgg gtgctcgtag aagcgatccg gcattttgcg caggctgagg cgcacgtcct
     2521 cgagactgat ttccttaccg acgatgtaga acggcacggt aggaaaacgc cggtgcatct
     2581 cgcgcatgac ccgtgcgagc accgtgccgt cgcccatgcc ggcgtcgaaa aggcgcaccg
     2641 acggtggtcg cggatggatg tggccgagct cgagtcccac acgcttcgcg actacgtcct
     2701 tctcgctgca cgtgttcacg aagagcaggt acttctgacg gttatcgaag aagcgaaagt
     2761 tcttttcggg cggcgcgtcg cggctctgta gcgcgaccgc ctgcacggcg cccggacgga
     2821 tcaagggcag caactcccgc ggcgcgtccc ctgcgccagg cgcgccatgc ttcaccatga
     2881 actggttgac ccggtccagg gtctcaggag tgacccgggc accgtcgcgc aggcgcgcga
     2941 cgaacttgcc gtcattgacg gcgcgccggc cgaaggtgga ctccgccatg ccggtgcgtc
     3001 ggcagaattc cgagatgctt cctaacaggc ttccgcctgc ggcgatgtcc atcgctcctc
     3061 cgctgtctat agtgggcaac gccatggtgg gacagcaacc acacgcgctt atgggatacg
     3121 tcccaccatt atgaatcaga tacttgaatt gataggcgag acgccgctcg tggaggtgcg
     3181 ccggctggcc gtcggccgct gccgcctctt cctcaagctc gagaaccaga atcccggcgg
     3241 gtcgatcaag gaccgtgtcg cgctcgccat gatcgaagcg gccgagcgcg acgggaaagt
     3301 ccagcccggc tcgctcatcg tggaggcgac ggcgggcaac accggcctcg gcctcgcgct
     3361 agtcgcggca caaaagggtt atcggctact catcgtcgtg cccgacaaga tgagccagga
     3421 gaagatcttc cacctgaaag cgatgggcgc tgaagtacag ctcacccgtt cggacgtgac
     3481 caaaggccat cccgagtact accaggacat ggcggcgcgg cttgcgcgcg agcatggcgc
     3541 gttctacgtg aaccaatttg ccaacgaagc gaatccgcgc gcgcacgagg aaggcaccgg
     3601 gccggagatc tgggagcaga tggagcacaa ggtcgacgcc gtggtcgccg gcgtcggcac
     3661 gggaggtacg ctcaccgggc tgtcacgcta tttcgcgcgg atctcgccgc aaaccgagat
     3721 ggtgctcgcc gacccggtcg gctcgatcct tgtcgagtac gtgcgcacgg ggaaggtggg
     3781 caaagccggc tcgtggctcg tcgaagggat cggcgaggat ttcattccct cggtcgccga
     3841 tctttcgcgg gtcaagaagg cgtacgcgat ctcggatggt gaagcgttcc tcacctgccg
     3901 cgagctcctg aaaaaagaag gcatcatggc cggcacctcg tccggcacgc tgatcgcagc
     3961 ggcgctgcgc tactgccgtg agcagagcga ggagaagcgc gtcgtctcgt tcgtggccga
     4021 cagcggcaac aaataccttt cgaaggtcta caacgactat tggatgatcg accagggatt
     4081 cattcagcgc gagcaatacg gcgacctgcg cgacctgatt gcccggcgcc accgcgagca
     4141 cgcggtcgcc acggtgaatg ccgacgagac ggtcctcgcg gcgcatcaaa gaatgaagct
     4201 ctacgacatt tcgcagctgc cggtgctgaa ggacggaaag atcgtcggca tcgtcgacga
     4261 ggaagacatt ctgctcgagg tgtacgacaa tccggcgcac ttccgggagc cggtcaccga
     4321 ggcgatggaa agccgcctcg tcaccgtgcc ccccagcgcg ccgatcgagc agctcatgga
     4381 aatcttcaac cgcgggctcg tcgccatcgt cgtcgacggc gatgaatttt tcggcctcat
     4441 cacgcgcatc gattttctga actggctacg gagaagagcc caatgaagtt cgagaccaag
     4501 gtgattcacg cgggccagga agccgacccg cgcacgggtg ccgtgatgac gccgatctac
     4561 gcgacgtcga cttacgcgca gtcgagtccg ggagagcacc agggcttcga ctacgcgcgc
     4621 acgcgcaatc ccacgcgcga cgccctggaa gcctgcatcg ccgaactcga gagcggcaaa
     4681 gcgggcttcg ccttcgcctc tggcatggcg gcgatcggca cggtgctcga gctactcgat
     4741 tccggctcgc acatcgtggc gatgaacgac ctctacggcg gcagctaccg gatactggag
     4801 cgcgtgcgca agcgctcggc gaaccttgcg gcgagcttcg tcgatctcac gtcgcttcaa
     4861 gcgttcgaga acgccatccg gcccgaaacg cgcatggtgt gggtcgagac gccgaccaac
     4921 ccgctcctca agatcgtcga tctggaggcg gtggcggcca tcgcgcgcaa gcgcaggctc
     4981 atctccgtgt gcgacaacac cttcgcctcc ccctggatcc agcggccgct cgagctgggc
     5041 ttcgacatcg tggtgcactc cacgacgaag tatctgaacg gccattccga cgtcatcggc
     5101 ggggcggctg tccttaaaga gaaaggcgag ctcgccgatc ggttgtcttt tcttcagaac
     5161 gcggtgggcg gcgtgccgag ccccttcgac gccttcctca cgctgcgcgg catcaagaca
     5221 cttgcgctgc gcatggagcg ccattgcggt aacgcaatgc atatcgccgc ctggctcgaa
     5281 aagcacccga aggtcgcgcg cgtcatctat cccggtcttg cgactcatcc gcagcacaag
     5341 ctcgccgcgc gtcagatgaa cgcccgctac ggcggcatgg tgaccgcggt gctgaaaggc
     5401 ggcaaggaag gtgcactgag cgcttcgaag cgcttcctcg agcgctgcaa gctcttcacg
     5461 ctcgccgaga gcctgggcgg agtggagagc ctgatcgagc acccggcgat catgacgcac
     5521 gcctcgctgc ccgaagatgt gcgcgccggc cttggcatcg acgacggcct agtgcgcctg
     5581 tcggtcggca ttgaaagcgt cgacgacctc ctcgccgagt tgaagtacgc gttggaatag
     5641 cgttgttgcg ctcgcactcc tcggcagtgc gctagccggc tttctcctat tcatgctccg
     5701 catgcccgga aagtcgtacg cggggccgct accgccgctc tccggcgaag cgcgggcgct
     5761 ctcgcttcgt ttgcgtctgc acgtggcagc gctcgccgcg ggggagcgca acgccgacct
     5821 ggagagaccg gcgcgctaca tcgagcaggc gttcgccgcg cagggctaca agacgcaggc
     5881 gcagaccttc cgcagcggcg gtcgcaccgt gcgcaacatc gaggcgggtg ccggtcccat
     5941 catcgtgggc gcgcattacg acaccgttcc gggctcaccc ggcgccgacg ataatgcctc
     6001 gggcatcgcg gtgcttctcg agcttgcggc gatgaaactg ccggttcgtt tcgtcgcttt
     6061 cgcgaacgaa gaagcgcctt actttctcgg cgaggaaatg ggtagctttg tctacgcccg
     6121 cgagcgtgcg gccggcgtgc gggcgatgtt ttcgcttgaa atgctcggct actaccgcga
     6181 tcacccgggc agccagcgtt accctgcacc gctcggattt ttctatccgg accgagccaa
     6241 cttcatcgct ttcgtcgccg atctgggcgc gcggcggctt ttgcaccgtt cgatcgaagc
     6301 ctttcgcaag cacgcgcagt ttccgtcgga aggcgtcgcc gccccttctt tcattcccgg
     6361 cgtgacctgg tccgatcact ggtcgtttcg ccagcacggc tatccggcga tcatgatcac
     6421 cgacaccgcg ttctaccgtt atccccacta ccacctgccg agcgacacgc cggacaagct
     6481 cgactacgag cgcatggcgc gcgtcgccct cgggcttgcc gcgatgctga aggagcttgc
     6541 caatgagagt cggtgatctc gaagtgaatc gcctgggctt cggcgcgatg cgtgtgctcg
     6601 gcacgccgga catctgggga ccgcccaagg atccggcgaa tgcgagaaaa gtgctgcgcc
     6661 gggcgtacga gctgggcgcg aacttcttcg ataccgctga gagctacggc ccgcacacca
     6721 atgagactgt gatcgccgag gcgatgcgcc cgtacccgaa ggacctcgtc atcgccacga
     6781 aatgcggcct ggtgcggccg aagccgagcc gctgggacga ggacgcgcgg ccggagaagc
     6841 tgcgcggaga cctcgaagga agcctgaagc gcctgaagct cgactgcctg cacctgtggc
     6901 agctgcacgc gcccgatccc agggtgccgc tcgaagaatc gctcggcgtg atggccgatg
     6961 cgcagcgcgc cggcaaggtg cggcacatcg gcgtgtcgaa cttcagcgtg aaagagctcg
     7021 cgcgcgcgcg caaggtggcg aaaatcgtgt cggtgcagaa ccgctacagc cttgacgacc
     7081 gcaacgagga agacgtgctc gcgcattgcg agaagctcgg gatcgccttc cttccctggt
     7141 acccgctcgg cgcgggaggc gccgtgcgcg cctcgaagat caagcgcgtt gcgaaaaggc
     7201 tcggcgccgc gccggcgcag gttgcgatcg catggctact tgccaaatcg ccggtgatgc
     7261 tgcccattcc cggcacgagc tcgctcgcgc atctcgagga gaacatggcg gcggcgaaga
     7321 tccagctctc tcgcgaagac ttggccgagc tcgactgatg gaagcggtgc tgccgattct
     7381 cctcgtcgcc cagggcgtgc tcgccggcgt ggacacggtc ctcaaccacg agctcctcgc
     7441 gcgcctgccg aagcgacatg cggttctcga cgagctccga gccaggcgta cgccgatctc
     7501 ggtcgcgaga agagcggaac gaccgcgtgc tgcacctttt ccttgccatc aacgtaggac
     7561 tgatcgtggc gtcggtgctc tcctggactg gcgagccgag cgcgctggtg cgccgcgatt
     7621 acggcgtgct ttcctgggtc atcacggggt tcggcgcgac ggccgcgttc tggtcggtgc
     7681 gcgacttcgt cgcatggcgc agcctgcggc gtgctagtgt tgcggcgaca tgaaaaccat
     7741 ccttctcgct ctgatgttgg tgaccggcgc ggccgccgcg caggaccaat atccctcgaa
     7801 gccgatccgc attctggtgg gctatgccgc gggcggcggc aacgacatta tcgtgcgcgt
     7861 actgcagccg gaaatgcaga aagggctcgg ccagccagtc atcgttgaga acaagcccgg
     7921 cgcgcagtcg atcgttgccg ccgagctcgc cgcgcgcgcc gcgccggacg gctacaccat
     7981 cttcatgggc ccgagcggcc cgatgaccat caatccggcg acctatgcca agctgcccta
     8041 ccacccgcag cgcgacttcg cgccgatctc catgatctgc gagtttccgc tcatcgtcac
     8101 cgtggacgcc aagctgccgg tcaagagcgt gaaggaactc atcgactacg cgaaggcgaa
     8161 tcccggcaaa tcgaactatg cgtcgagcgc gggcatcttc cagatcacca ccgagctgtt
     8221 caaacagaag accggcacct cgatcgagat gatcccgtac aagtcgagcg gcgaatcggt
     8281 gcaggccgtg gtgggcggga cggtgatgat gaccatcgtc gatccgccgc cagcaacggg
     8341 cccgctcaag gccggcgcgc tgcgcgggct ggctgtcact tcgggcaagc gccatccggc
     8401 gtggcccgac attcccacta tggcggaagc cggcgtgccc gacatggaag tcccggtgtg
     8461 gacggctttc ttcgcgcccg ccaagactcc gccagcgatc gtcgcccgcc tgcaaaaaga
     8521 ggtcgcccgc gccgtgcaga cgccggaagt gcgcgagcgc ttcgcgcaga tggcgctcga
     8581 gccggtcggc ggcagctccg aggatctcgg gaaacgagtc gcgcgcgaca tcgagaagtg
     8641 gaccgccgtc gcgaaggcgg cgaacatcaa gaacgactga aaccggggtc agagcccgat
     8701 tgacttacac cagttggcgg gcgatgcgca gccgcgtctc gcgcatgtcg tcgccgcgca
     8761 gctcgcgcag cgcatagcgc gcgagttcta ccgcgacgtc cttgcgccag tagagatcga
     8821 ggtcggcatt gtccatcggc ttcgcccgcg aggcgacgag ctttgcggct tcgtcgatga
     8881 cttcgtcggt gagctcctta ccctccagga aatcgttcgc cttgtggacg aggaacggtc
     8941 gcgaggccac cgcgccgagc gcgatgcgca cgccttgggg tgacgccgct gcggcaacgc
     9001 cgaggaccgg gaaatcgaag gcgccgcgcc ggcgcagctt ccaataggtg ctcgccgagc
     9061 tgggcggtat cagcacttcg gtgaggattt catcgggccg gcgtttcagg tagtccatgc
     9121 cgtcgttgtt gtatagctct tcgagaccga tttccctttc cccactggaa ctaacaaggt
     9181 gcacgcgggc gccgagtgcg aggagcgccg gcgcgcagtc ggtggatgaa gcggcgacgc
     9241 agcgcttgct cgcggtcgcg acccagcaga catcgccatc cttcttgagg cagaagtcga
     9301 tcgccttacg ccactcgtag ttctggttgt agtagttgca gcgcgtatcg aggcacaggt
     9361 ttccgccgag cgttcccatg ttgcgcaggt gcacggtggc cacctgatgc gcggcctggc
     9421 gaagtgcttt cggcgcattc gagttcacga cctgcgtgag cgtgaggccc gcgccgtaga
     9481 cgctgccgtt tcgcttgagc gcatcgatgg aagaaagcga aacgagcatc ttcggcgcca
     9541 tctggcggcg cttcatgttc ggcaggagat cggtgccgcc ggcgatcagc atcgcgtcgc
     9601 cctcgcccgc gaggatgcgc gccgcctcag ccaccgattg cggcgcgcga tactgaaacc
     9661 acggcaggcg catcatgcgg cgctgacctt cagcttcgtg cgctcgtggc ctttgacggc
     9721 gttgccgtcg ccgccttccc atggcggcgg cacgatgacc ggctcggggt acggcacggc
     9781 cggaaacgaa gtcggcccca cacgcggcgt cttgcctgca gctttcagct cgagcgcgcg
     9841 caataccttg tcaggcgtga tcggaagctc gtcgatgcgc acgccgaccg cgtcgtagat
     9901 cgcgttcgcg acagccggca tcaccggcag aagcggtccc tgccccactt ccttcgcgcc
     9961 gaacgggcag ttcggatccg gatcctcgat gagcacggta tccacttccg gcatgtcgag
    10021 cgaagtgagg ctcttgtatt cgaggagcga aggaatcttg tggacgagcg cgcgcgagag
    10081 cttgggcggc aggcggcgaa acacctgttc ttccataagc gcctcggaca ggcccatgta
    10141 gacgctgccc tcgacctgcc cgcgcgcgag caccgggttg atggtgcggc cgatgtcgtg
    10201 cgcgatccag atcttcggca cggtgatcca gccggtggcg ggatcgacct cggcttccac
    10261 cacgcacgcg gtgtacgagt aagccggcga aggtcccacg ccggcgccct tgtagcgtcc
    10321 cggcgcgcgc ggcggcttgt aggagccgag cgcaccgagc gttccgaatt tttcctcggc
    10381 gaagctgacg gcctcggcga acgtgacgct cctgccgtcg aaggccacgc ggccattggc
    10441 gaacacaagc ttgtctggcg cgacttccag ttgctccgcc gtggcctgcg cgatcaggtt
    10501 tttgactttg ttcgaagcat cgatcgcggc attgcccatc atcacggtaa cgcggctcga
    10561 gtacgagccg aggtccaccg gcgtgatgcc ggtgtctccg gtcacgcagc gaatatcgaa
    10621 cggatcgatg ccgagcactt cggcgatgat tgcggcgagc acgtcgtcgg agccctggcc
    10681 gatctcggtc gcgccgcaga acgccgtgac ctggccgctg cggtcgacga gaagctgcac
    10741 tcccgactgg ggcatcttgt tgaagtagat cggcagcccg gctccgcaaa ggtacgaacc
    10801 gcacgcaagg ccgaggcccc ggccagggcc gagcttgcgg aaccggctct tccacgcgga
    10861 gcgttccacc accgtgcgga tgcactgctc gaggccgatc gagccgatct tcagccagtt
    10921 cgccgtgagc gaattgggct tcgccaccat attcaatcgc agctcagccg ggtcgatcgc
    10981 cagcttctcg gcgatcttgt cgagctgcac ttcctgtccg aagcgcggct gcggcgtgcc
    11041 gtggccgcgc ttcgggccgc acggcggctt gttcgtgaaa acgcgcgccg cttcgaactt
    11101 gtagcgcggc acttcgtacg tcacggtctg aagcgcgccg gtatagaagg tactcgccgt
    11161 cccatagctg ccgtagccgc cgccgtcgat cagcgtctgt aggtgcatgc cggtcagctt
    11221 cccgtccttc gtcacgccgg tgcgaaactt catgagcacc gggtggcggc cgcgatgcat
    11281 gtagaacact tcctctcgat tcaggcagat cttgacgggg cgaccgagca ggatcgcggc
    11341 cttgcccacg accatctcgt ggttgcacac atcgcacttg ccgccgaagc cgccgccatt
    11401 cggcgtcgcg atcacgcgga tgtgcgaggc gggcagctga agcacgcggg cgagcgagcg
    11461 gtgcacgtag tgcggcacct gggtcgacga ccagagcgtg agcttgcctt caccgtcgac
    11521 cgcggcgacg gatgcgtgct gctcgatcgg caggtgggtg ttgccctcgt agaagaaaag
    11581 atcctcgaag acgtggtctg atctttccag cgcttcgtcg acgtcgccga actcgaacgc
    11641 ctgcagccgg tggatgttgc cgagctcgct gtaggcatgg atgcgcggct cggggttctg
    11701 gagcgcctcg accggatccg aaatcgtctg cagcggccgg tacttcactt ccatcagatc
    11761 gcacgcttcg gctgcagtct gctcgtcgcg cgccaccacc gccgccaccg ggtcgccgac
    11821 gtagcgcaca tgctgcggcg caagcgggta ttcgtcctgg ctcaccggca gaatgccgaa
    11881 ggtcaccggg aaatcctggc ccgtaagcac gaggtggacg cccgggtgcg ctttcgctct
    11941 tgcgatgtcg atcgactcga tgagcgcgtg cggctgcggg ctgcgcagga gcttcatgtg
    12001 cagcatgcgc ggcagggcta tgtcgtcggc gaagcgcgtc tgacccgtca ccttggcgcg
    12061 cccgtcgacg cggcggcgcg gtttaccgag aaccttcatc gcgcattctc ttcgccgcgt
    12121 cttcgatcgc ttcgaaaatc tgctggtagc cggtgcagcg gcatagattg cccgagatcg
    12181 cttccttgat gcgctcgcgc gagggctccg gttccttttc cagaagggcc ttcgcggtca
    12241 tgagaatgcc cggcgtgcag tagccgcact gcgcggcgcc gaggtccgcg aacgcggcct
    12301 ggagcgggtg gagcttcggc ccgtcggcga ggccttcgac cgtgtcgacg cgccggccct
    12361 ggcagtcgag cgcaagcacc aggcacgcga gcttcggctc gccgtcgacg agcaccgcgc
    12421 aggcgccgca ctcgccgagc tcacagccgt gcttggtgcc ggtgaggttc aggtcctcgc
    12481 gcagcacttc gagaagcgtc ttgtaggggg cgaagaaaac cgcggccggc tcgccgttca
    12541 cggtgagact gagctcgacc tggcgcattg tttgggaccc aaacaaacgc cgctactcta
    12601 cacggtttcc tgcgtgagcg atgccgtggt ggacgcgctc gccccgcatt gagtagaatt
    12661 tttgccccgc atcgttttct tcccgaggta cccccatggc acttcgcatc ggcgacaccg
    12721 ctcccaactt ccaggcgcag accacccaag gcccgatcga cttccaccag tggatcggcg
    12781 agagctgggc catcctgttc tcgcatccga aggacttcac gcccgtctgc accaccgagc
    12841 tcggctacat ggcacgcatc aaacccgagt tcgatcggcg caacgccaag atcatcggcc
    12901 tctccgctga cccggtagag gaccacgcca agtgggcgaa ggacatcgag gagacgcaag
    12961 gcgcggcggt gaactacccc atgatcggcg atcccgacct gaagatcgcc aagctctacg
    13021 acatgcttcc tgccgaggcg accccgggga agcgtacgcc ggccgacaat gcgaccgtgc
    13081 gcaccgtgtt catggtcggg cccgacaaga aaatcaaact catgctgttc tacccgatga
    13141 ctacgggccg caacttcgac gagatcctgc gcgcactcga ttccatgcag ctgacggcga
    13201 agcacaaggt cgccacgccg gcgcagtgga agcagggcca ggacgtcatc atcgctggct
    13261 ccgtcagcga cgaggacgcg aaaaagcttt tcccgcaggg atggaaggcg ccgaagcctt
    13321 atttgcgaat cactcaacaa ccgaaatagg cgaggtccgg ccccttttct gaaagaaggt
    13381 cctgacccct tttggcgctc gggagtattt ggcggaagtc caccgagcga ctttcgcgcc
    13441 gaattgcaga agaggagcgg catcagctcg cacggccttc ttaaacacgc gccggtagca
    13501 agacgcggaa ggtagaaccc ttaccgacgg cgctgtcgac caagacttgc ccgccgtgca
    13561 gctcgaccag gcgctttgta agggcgaggc ccaagcccgt gccttcggcc ttgcggctgc
    13621 tgtccttgcc aagctgcttg aactcctcaa agagcgaggc ctgatcctcg ggcgctatcc
    13681 ccactccggt gtccttcacc gcgatctcga ctttgtcggt gtcgagcttc gcgctcacgt
    13741 ccacccgccc accgtcaggc gtgaacttga cggcgttcga tagcaggttg agcaggatct
    13801 gcttcacctt gcgctcgtcg gcccgaaact ccccaaggcg ctggtcgatg tccatgacta
    13861 actcaattcc gtggcgcatc gcccgctcgc ggacgaggat tactgcgttg gaaactgccg
    13921 taggtaggtg gaagttcgat agctccagtt ccatgcggcc agcctcgatc ttagagaggt
    13981 cgaggatgtc gttgatgagc gaaagcaggt gcttgccaga ctcgtggatg tccttctggt
    14041 attcgagctg cttctcgttt aggtcgccga acatgcggtc catcaacgct tctgaaaagc
    14101 cgatgatcgc gttgagcggt gtgcgcagct cgtgcgacat gttggcgagg aagtcggatt
    14161 tgtgcttgtt cgcaacctcg agctccgcgc ttttctcctg gatctcgcgg aacaggcgcg
    14221 cgttctggat cgcgatcac
//
LOCUS       DASTPD010000249         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_128056, whole genome shotgun sequence.
ACCESSION   DASTPD010000249 DASTPD010000000
VERSION     DASTPD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128056"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VFR57_08270"
     CDS             <1..1746
                     /locus_tag="VFR57_08270"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0259396.1"
                     /translation="NEVLRKMVLGMVEDVRVVLIRLASRTQTLRYFAKSSSAERLSYA
                     RETLDIYAPLANRLGAWQLKWELEDLSFRFLEPELYKRIAQMLDEKRLERERYIADTI
                     EALARELGTVDVKAQITGRPKHIYSIWNKMRTKQLDFSELYDVRALRVIVPEVKDCYA
                     ALGVVHNLWQPIPKEFDDYISRPKGNLYQSLHTAVVGPGGKTVEVQIRTEEMHRQAEY
                     GVAAHWQYKEKTKPSQQFDQKVAWLRQLLAWRDEVPVQKPQLDDTIYVLTPQGKVIDL
                     PAGSTPIDFAYALHSDLGHRCRGARVDGHIVKLDTPLANGQRVEIVAAKSGGPSRDWL
                     NLERGFVKSARARHKIRQWFNAKAFAETVAAGRAVVDKELRRVGATQASLEGLAAKLG
                     FGKPDELFAAVARDEVNLRQLQIALKGAAPAAPEVKKPRKAPSAKAAMLVVGMGGLAT
                     QLARCCKPVPPDPIRGFVTRGKGVSIHRDGCESLKRLAEQHPERVIETAWGRSEAAYA
                     VDMVVTAADRRGLLRDIGDALARESINVTAVRTQSRDELAFMRFSFNVADAAQLKRAL
                     ALVRSVKGVIRVARA"
     gene            1861..2580
                     /locus_tag="VFR57_08275"
     CDS             1861..2580
                     /locus_tag="VFR57_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259397.1"
                     /translation="MVAGFCATAWLMGVLEPSTRTERQRFVLLALAAPALGLVADFAF
                     RAGRMTAYVLGAAFGGAALWAFGAAIMQKPPVPAFILGGGIVLFVAWTVALTASLHAN
                     SVRGGAAALGLGLGAGFAALLSGGPLAHLGIALGAAAGGFLVLQMLLGQRIDAGLVFC
                     LAAGVQGALVAVAALTLGRLDWIALAILAAVPIAARIPLPTRWPVWGQTLLTSLYTLA
                     CGGAAVAATYVASRGWPSWLQ"
     gene            2603..3016
                     /locus_tag="VFR57_08280"
     CDS             2603..3016
                     /locus_tag="VFR57_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0259398.1"
                     /translation="MNSAAQSQTLLIGRVLLALVFLVAGIRIILSYSGNIGYFTKLGF
                     PAPEVMTVLAILIEIGGGVLLILGWQTRRISWLLVLFVIIATAMAHRFWEFDAAQYGN
                     QLNHFLKNVAIIGGLLYVIVFGAGRLSLDERSATS"
     gene            complement(3022..3351)
                     /locus_tag="VFR57_08285"
     CDS             complement(3022..3351)
                     /locus_tag="VFR57_08285"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU0259399.1"
                     /translation="MLNAAHYSLSGLAAAARHEDAFRQELILVALLTPAALWLGDTGV
                     ERALLIGSLVLVLIVELLNSAVEATVDRISFENHRLAKRAKDIGSAAVMLSLANTGVV
                     WLLIILS"
     gene            complement(3390..5570)
                     /gene="ppk1"
                     /locus_tag="VFR57_08290"
     CDS             complement(3390..5570)
                     /gene="ppk1"
                     /locus_tag="VFR57_08290"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU0259400.1"
                     /translation="MESEGLGKTPERIVPPASPDITIPVPPSLSGKPVHPVNRRTTLP
                     AQFLNRELGILAFNRRVLAQAADDTVPLLERLRFITIVSSNLDEFFEIRVAGLKEQIK
                     LGLPEPGPDGRAPTEVFDAVSREAHTLVAEQYQLLNQTVLPALAAQGIVFPKREEWNA
                     AQRSWIREYFFRELLPVLTPIGLDPAHPFPRVFNKSLNFAVELDGRDAFGRNSGAAIV
                     QAPRALPRAIRLPQAIAGEAEYAFVFLSSILHAHMDELFSGMKVQGCYQFRVTRNSDL
                     FVEEEEIKNLHTALQGELFHRHFGDAVRLEVADNCSQRMVEFLLQQFGLREADLYRVE
                     GPVNLYRLREVPDQVERPEVKYPPFQPGLPPALDTGDMFDVLKKQDILLHHPFQSFLP
                     VIDFIRTTANDPNVIAIKQTVYRTGTDSELMEILVEAARKGKEVTVVVELMARFDEEA
                     NLNWAARLEEVGAHVVYGVVGHKTHAKMALVVRREEGALRRYAHLATGNYHPRTARLY
                     TDFGLLTANEEICADVNEVFKQLTGLGRAGKMKHLWQSPFTLHKRILAAIRNETAQAA
                     EGKPARIIAKMNALLEPQIIEELYAASAAGVKIELIVRGVCALKPGIEGLSENIRVRS
                     VVGRFLEHTRIFFFHNGGAEDVYLASADWMDRNFFRRIELCFPVLDPALKRRVIREGL
                     QPYLDDNCQVWVMNADGGYERLKPRRGRRRSAQEELLFTLGIPT"
     gene            5764..5931
                     /locus_tag="VFR57_08295"
                     /pseudo
     CDS             5764..5931
                     /locus_tag="VFR57_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
     gene            5956..>6467
                     /locus_tag="VFR57_08300"
     CDS             5956..>6467
                     /locus_tag="VFR57_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU0259401.1"
                     /translation="MARHETLAAVDLGSNSFHLQIGRVVDGQIYPLDAVREVVRLGGG
                     LTAEKRIDRATQAAAFEALAKFGERLRGFPKAAVRAVGTNALRVAKNAPQFLREARAT
                     LGFPIEVISGREEARLIYLGVANSMPASRSETPARRLVVDIGGGSTEFIIGTGLEPLL
                     TESLYMGCVS"
BASE COUNT         1078 a         2068 c         2174 g         1147 t
ORIGIN      
        1 aacgaggtac tgcgcaagat ggtcctcggc atggtggaag acgtgcgcgt cgtgctgatc
       61 cggctggcga gccgcaccca gacgctcagg tacttcgcca agagctcgtc tgccgaaagg
      121 ttgtcatatg cacgcgagac cctggacatc tacgcgccgc ttgccaaccg gctcggggcg
      181 tggcagctca agtgggagct cgaagacctg tcgttccggt ttctcgagcc cgagctctat
      241 aaacgcatcg cgcagatgct ggatgaaaag cgcctcgagc gcgagcgcta catcgcggac
      301 accatcgagg cgctcgcgcg cgagctgggg acggttgacg tcaaggccca gattaccggc
      361 aggccgaagc acatctactc gatctggaac aagatgcgca ctaagcagct ggatttctcc
      421 gagctctacg acgtgcgcgc gcttcgcgtg atcgttcccg aagtgaagga ctgctacgcg
      481 gcgctgggcg tggtgcacaa tctctggcag ccgatcccga aagagttcga cgactacatc
      541 tcgcgcccga agggcaacct ctaccagtcg ctgcacaccg cggtagtggg tccgggcggc
      601 aagacggtgg aagtgcagat ccgtaccgag gagatgcatc gccaggccga gtacggcgtc
      661 gcggcgcact ggcagtacaa agagaaaacc aaaccttccc agcagttcga ccagaaggtc
      721 gcctggctgc ggcagctgct cgcctggcgg gatgaggtgc ctgtccagaa accgcagctc
      781 gacgacacca tctacgtcct caccccgcag ggcaaggtca tcgacctgcc ggcgggctcg
      841 accccgatcg attttgccta tgcgctgcac agcgacctcg gtcaccgctg ccgcggcgcg
      901 cgcgttgatg gccacatcgt caagctcgac acgccgctag cgaacggcca gcgtgtcgag
      961 atcgtggccg cgaagagcgg cggcccctcg cgcgattggc tcaacctcga gcgcggcttc
     1021 gtgaagagtg cgcgcgcgcg tcacaagatc cggcagtggt tcaatgccaa ggcgtttgcc
     1081 gagacggtgg ctgcgggccg tgccgtggtc gacaaggagc tgcgccgggt gggcgctacg
     1141 caggcgagcc tggaggggct cgcggcaaag ctcggtttcg gcaagcccga tgagcttttc
     1201 gccgcggtgg cgcgcgacga ggtcaacctg aggcagctgc agatcgcatt gaaaggcgct
     1261 gctccggcgg ctccggaagt caagaaaccg agaaaggcgc cgagcgcgaa agcggccatg
     1321 ctcgttgtcg gcatgggcgg gcttgcgacg cagctcgcgc gctgctgcaa acccgtgccg
     1381 cccgacccga tccgcggctt cgtcacgcgc ggcaagggtg tttcgatcca tcgcgacggc
     1441 tgcgagagcc tgaagcggct cgccgagcag cacccggagc gcgtgatcga gacggcctgg
     1501 ggcagaagcg aggcggctta cgcggtcgac atggtggtga ctgccgccga tcgccggggg
     1561 ttgttgcgcg atatcggcga tgcgctggcg cgtgagagta tcaacgtcac cgcggttcgc
     1621 acgcaatcgc gcgacgagct cgctttcatg cgcttcagct tcaacgtcgc ggacgcggcg
     1681 cagctgaagc gcgcgctggc gctcgtgcgc agcgtcaagg gcgtgatccg tgtggcgcgc
     1741 gcgtgacgaa cgaagctgtc gatcattaac gatcctttct ttcaggcggg gcttgcaccc
     1801 ttcctggcag gattcatagt cgcgctcgtc ctgttcaagc tgcgcctcgg cggcctcgcc
     1861 gtggtcgcgg gcttttgcgc caccgcgtgg ctgatggggg tgctcgagcc ctcgacccgc
     1921 accgagaggc aaaggttcgt gctgctcgcc ctcgccgcgc cggcgctcgg tctcgttgca
     1981 gatttcgcct tccgcgccgg ccgcatgacg gcgtacgtcc tcggtgcggc gttcggcggt
     2041 gccgcgctat gggcgttcgg ggcagcaatc atgcagaaac cgccggtgcc ggcgttcatc
     2101 cttggcggcg gcatcgtctt gttcgtcgcg tggacggttg ccctcaccgc ttcgctgcac
     2161 gccaactcgg ttcgcggagg cgccgccgcc ctggggctcg gcctcggcgc cggattcgcc
     2221 gcgctcttga gcggcggtcc tttggcgcat ctcggaatcg cgctcggcgc ggcagcaggc
     2281 ggatttctgg tgctgcagat gctgctcggg cagcgcatcg acgccgggct cgtgttctgc
     2341 ctcgcggcgg gcgtgcaagg cgcactcgtc gccgtggctg cgctcacgct cggccgcctt
     2401 gactggatcg cgctcgcaat cctcgcagcc gtgccgattg cggcacgcat cccactgccg
     2461 acgcgctggc cggtatgggg acagaccctt ctcacctcgc tctatacgct cgcatgcggc
     2521 ggtgcagcgg tggcggcgac gtacgtcgca agccgcggct ggccgtcgtg gctacaataa
     2581 cctcacaagg agggaggact acatgaactc agctgcgcaa tcgcagacgt tgctcatcgg
     2641 acgcgtgctg ctcgctttgg tttttcttgt cgccggcata cgcatcatcc tgtcctattc
     2701 cgggaacatc ggatacttca ccaagctcgg cttcccggcg cccgaagtaa tgacggtgct
     2761 cgctatcctg atcgaaatcg gcggaggcgt gctcctcatc ctgggctggc agacgcgccg
     2821 catttcctgg ctgctggtgc tcttcgtgat catcgccacc gccatggcgc accgcttctg
     2881 ggaattcgac gcggcacagt atggaaacca gctgaaccac ttcctcaaga acgtggcaat
     2941 cattggcggg ctgctgtacg tgatcgtctt cggtgcgggg cggttgagcc tcgacgagcg
     3001 ttcggcaacc agctagccgg tctatgaaag gataatcagg agccagacga cgccggtatt
     3061 ggcgagcgac agcatgactg cggcgctgcc gatgtccttt gcgcgcttcg ccagccggtg
     3121 attctcgaag gagatgcggt cgaccgtggc ttccaccgcc gagttgagca gctcgacaat
     3181 gaggacaagg accaggctgc cgatcaggag ggcgcgctcg acgcccgtgt cgccgagcca
     3241 gagcgccgcg ggggtgagga gagcgaccag gatgagctct tgccgaaacg cgtcttcgtg
     3301 ccgggccgct gcggcaaggc cggaaaggga gtaatgcgcc gcgttgagca cgcggcgcag
     3361 cccggtcttg cccttgtgcg ggctttcttc taggttggta tcccgagcgt gaatagcagt
     3421 tcttcctggg cggagcggcg tcggccacgg cgcggcttga gtctctcgta tccgccgtcg
     3481 gcgttcatca cccagacctg gcagttgtcg tcgagataag gctgcaggcc ctcgcggatc
     3541 acccggcgct tgagcgccgg atcgagcacc gggaagcaca gctcgatgcg gcggaagaaa
     3601 ttgcggtcca tccagtccgc gctcgcgaga tacacatcct cggcgccgcc gttgtggaag
     3661 aagaagatgc gcgtgtgctc gaggaagcga ccgaccaccg agcgcacccg gatgttctcc
     3721 gagagccctt cgatgccggg cttgagcgcg cacacgccgc gcacgatgag ctcaatcttc
     3781 acgccggcgg cggaggcggc gtaaagctcc tcgatgatct ggggctcgag cagcgcgttc
     3841 atcttggcga tgatgcgcgc gggcttccct tcggcagcct gcgccgtctc gttgcggatc
     3901 gccgcgagaa tccgcttgtg cagcgtgaag ggcgattgcc agagatgctt catcttgccg
     3961 gcgcgtccga ggccggtaag ctgcttgaac acctcgttca cgtcggcgca tatctcctcg
     4021 ttcgccgtca gcagcccgaa atcggtgtaa agcctggcgg tgcgcgggtg ataattcccg
     4081 gtcgcgaggt gagcatagcg tcttagcgcg ccttcctcgc gccgaaccac gagcgccatc
     4141 ttggcgtgcg tcttatgccc gaccacgccg tacaccacgt gcgcgccgac ctcctccagg
     4201 cgcgctgccc agttgaggtt cgcctcctcg tcgaagcgcg ccatcagctc caccaccacg
     4261 gtgacttcct tgcccttgcg cgcggcctcg accaggatct ccatcagctc cgaatccgtg
     4321 ccggtgcggt acaccgtctg cttgatcgcg atcacgttag ggtcgtttgc cgtggtgcgg
     4381 atgaagtcga ttaccggcag gaacgactgg aaggggtggt gcagcagaat gtcctgtttt
     4441 ttcagcacgt cgaacatatc gccggtgtcg agcgcgggcg gcaggcccgg ctggaacggc
     4501 gggtatttga cctccgggcg ctcgacctgg tcgggtactt cgcgcagccg gtagaggttg
     4561 accggaccct cgacgcggta caggtcggcc tcgcgcaggc cgaactgttg cagcaggaac
     4621 tccaccatgc gctgcgagca gttatcggcg acctcgagcc gaaccgcgtc gccgaaatga
     4681 cggtgaaaga gctccccctg cagcgccgtg tgcaggttct tgatctcttc ctcctcgacg
     4741 aagaggtcgg agttgcgcgt cacgcggaat tggtagcagc cctgcacttt catgccggag
     4801 aagagctcgt ccatgtgtgc gtgcaggatg gacgacagaa aaacgaacgc gtattcggcc
     4861 tcgccggcga tcgcctgcgg caggcggatc gcgcgcggaa gcgcgcgcgg cgcctgcacg
     4921 atcgccgccc ccgagttgcg gccgaacgcg tcgcggccgt cgagctcgac agcgaagttc
     4981 aggctcttgt tgaacacgcg cgggaacggg tgcgccggat ccaggccgat gggggtgagg
     5041 accggcagca gctcccggaa gaaatactcg cggatccagc tgcgctgcgc ggcgttccat
     5101 tcttcgcgct tcgggaacac gatcccctgc gccgccaggg ccggaagcac cgtctggttg
     5161 aggagctggt actgctccgc cacgagcgtg tgcgcctccc ggctgaccgc atcgaaaacc
     5221 tcggtgggcg cgcgcccgtc ggggccgggc tcgggcaaac cgagcttgat ttgctccttg
     5281 aggcccgcca ccctgatctc gaagaactcg tccagattgg acgacacgat agtgatgaag
     5341 cgcagccgct caagcagcgg aaccgtatcg tcggctgctt gcgccagcac gcggcggttg
     5401 aatgccagga tgccgagctc gcggttaagg aactgcgcgg ggagggtggt tcggcggttc
     5461 accgggtgca ccggcttccc cgagagggaa ggcggaaccg ggattgtaat gtccgggctt
     5521 gccggaggaa cgatgcgctc cggcgtcttt cccagtcctt cggattccat gcgaaccgcc
     5581 ccctgagcgc aattctgcac atccgcggcc tgtttttgat gactgttttg ttaccgccgc
     5641 caatcccatg aagcaacgcg gaaatatccc ggaaagaccc cgccgatgca ggcaaaccag
     5701 gccgtcgagg cgctcgaccc gaatgcggcc gccgcgcgct ggtgcggcgc gacctcgaag
     5761 tgagatccgc gggcgatctc cgcctgcctg gatatcctct tcgtcgccaa gtcgctcgag
     5821 cgcatcggcg atcacgcgaa gaacatcggc gagcacgtgg tgtacgcgtc gatggggaaa
     5881 gacgtgcggc acgccagcgt tcacgagctg gagcgactgt tacagaaata gagccgttct
     5941 gctaacgtgg gcgcgatggc gcgccacgag acgcttgccg cagtcgacct cggttcgaac
     6001 agcttccacc tccagatcgg ccgcgtcgtg gacggacaga tctatccgct cgacgcggtg
     6061 cgcgaggtgg ttcggctcgg cggcgggctg accgcggaga agcgcatcga ccgtgccacg
     6121 caggcggcgg cgttcgaggc gcttgccaag ttcggcgagc ggctgcgcgg ttttcccaag
     6181 gccgcggtgc gcgcggtcgg caccaacgcg ctgcgcgtgg cgaagaacgc gccgcagttc
     6241 ctgcgcgaag cgcgcgcgac gctcggcttc ccgatcgagg tgatctcggg ccgcgaggag
     6301 gcgcgtctca tctacctcgg cgtcgcgaat tccatgcccg catcgcgatc ggagactccc
     6361 gcacgtcggc tcgtggtcga catcggcggc ggctcgacgg aattcatcat aggcacgggc
     6421 ctggagccgt tgctcaccga gtcgctctat atggggtgcg tgagcta
//
LOCUS       DASTPD010000250         6877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_334301, whole genome shotgun sequence.
ACCESSION   DASTPD010000250 DASTPD010000000
VERSION     DASTPD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334301"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFR57_08305"
     CDS             complement(<1..448)
                     /locus_tag="VFR57_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259402.1"
                     /translation="MLVRVLAVCCLVGYAHDALAARVEIPLRVSLDTLGEALGAQLVP
                     YRDGRCRYLELQAPKLEFAQGRLRLVAPGTGSLAVEIAGKCQGTAWKGTIHLALVPYV
                     EDAARLRLRVVDSRLVDASGGLGPLLWDAAKQHIHARLERFAYELGA"
     gene            588..767
                     /locus_tag="VFR57_08310"
     CDS             588..767
                     /locus_tag="VFR57_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259403.1"
                     /translation="MRQLLRGARTLSPYLLTEALLPGGTLIALGLWLYQNRGRLPKWG
                     RASGNSDASSTSTAL"
     gene            810..1412
                     /locus_tag="VFR57_08315"
     CDS             810..1412
                     /locus_tag="VFR57_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEU0259404.1"
                     /translation="MAKSSKAWLRRHITDPYVKQARKQGYRSRAAFKLLEIDSKENLF
                     RPGQTVIDLGAAPGGWSQVSAEKIGRGGRVIAIDLLEIAPISGVTVHRGDFRQINLQL
                     AFNAQADVVLSDLSPSLSGIPNVDQARWLELAEGALQLCRKVLKADGVFVVKAFHGEA
                     FDALMRRMKGFFSKVKVVKPSASRGESAETYVVARALRPL"
     gene            1461..3344
                     /gene="ftsH"
                     /locus_tag="VFR57_08320"
     CDS             1461..3344
                     /gene="ftsH"
                     /locus_tag="VFR57_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0259405.1"
                     /translation="MLKNLVIWLVIGLVLMTVFNQFNTRQSAQAPMEYSQFLEEVKAG
                     RIAKVTIEGRQLKATTSEGKRVTSYSPGDIWLVSDLLKYGVKIEAKPEEEPSLLMNIF
                     VSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNTVTFADVAGCEEAK
                     EEVSELVDFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFSISGS
                     DFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQ
                     LLVEMDGFESTMGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREEILKVHM
                     RKVPLAPDVKPDILARGTPGFSGADLANLVNEAALFAARGNKRLVDMDDFERAKDKII
                     MGAERRSMVMPEEERRNTAYHESGHAIVARLLPKTDPVHKVTIIPRGRALGVTMQLPE
                     TDRYSQDRDRLMNMIAVLFGGRIAEEIFMNQMTTGASNDFERATEIARRMVTQWGMSD
                     ELGPMVYGENEGEIFLGRSITTHKNVSEATMQKVDAEIRRIIDQQYRLARKLLEDNRD
                     KVEAMAKALLELETIDADQIGDIMAGKPPRPPKPSGSAQSAPKPPAGEGGAEGAAAPT
                     PAA"
     gene            3418..4188
                     /gene="folP"
                     /locus_tag="VFR57_08325"
     CDS             3418..4188
                     /gene="folP"
                     /locus_tag="VFR57_08325"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0259406.1"
                     /translation="MGVVNVTPDSFSDGGRFLDRAAAISQARRLSDEGADIVDIGGES
                     TRPGADQVSEEEELRRVIPVLDKIADLCVSVDTRHPRVMREALQAGASMINDVDALRA
                     PGALEAVAGSSCGVCLMHKKGEPATMQREPHYDDVVGEVKAFLGQRLDAARRGGVAAE
                     RIVVDPGFGFGKTVAHNLELLRRLNALAALNAPMLVGLSRKSTLGNITGRPVEQRLAG
                     SLAMALLALQGGAAILRVHDVKETKDVVAVWRAYQNDA"
     gene            4238..5575
                     /gene="glmM"
                     /locus_tag="VFR57_08330"
     CDS             4238..5575
                     /gene="glmM"
                     /locus_tag="VFR57_08330"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0259407.1"
                     /translation="MKRKHFGTDGVRGTIGESPMTPDFVLRLGYAAGKVMSAQGGHSA
                     VLIGKDTRISGYMVESALEAGFSAAGIDVHMCGPMPTPAVAYLTRALRLAAGVVISAS
                     HNPYPDNGIKFFSGDGFKLPDDTEARIEVEMEQPMGCNEAAKLGKVWRVDDAPGRYIE
                     FCKSTFPTQLDLKGLRIVVDCAHGAAYHVAPHVFHELGADVTPIGVEPDGFNINDGFG
                     ATSPDCLKKHVLLQKADLGIALDGDGDRVLMVDAAGRSYDGDQLLFAIAKHRSGRQKV
                     AGVAGTLMTNYAFEKAMARLGIPFARARVGDRYVLELMRERGWQLGGENSGHLICLDK
                     HTTGDGIVSALQVLAAMRESRRALAELTAELVMFPQVLVNVAVPRGFQWEKHEAIKKA
                     QSDVERSLDGKGRVLLRPSGTEPVLRVMVEGEPREAIESAAKSIADAVKQAAR"
     gene            5608..6726
                     /locus_tag="VFR57_08335"
     CDS             5608..6726
                     /locus_tag="VFR57_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259408.1"
                     /translation="MAQTLAWGSTYYLPAILANSIAGELGVATAWVFTAFSCGLLLSA
                     TLGPAAGRLIDVYGGRRVLPVSNLVFAAGLALLGSCQGVAGMFASWLVIGAGMGAGLY
                     EAAFSTLAGIYGQEARRSITGITLIAGFASTVCWPISAYLDVTIGWRATCYVWAAAHV
                     ALGLPINLLLPRPRRAEKSGQSQARASSGRGRLAMMAGLAFVFAATWFCSTAMAAHLP
                     RLLQESGASLAAAVAAAALVGPAQVAARVLEFWFMRRFHPIVSARVASLAHPIGAAGL
                     IAAGSPAASGFAVLHGAGNGVMTISMGTLPLALFGAAGYGLRQGLMIAPARFVSASAP
                     FLFDLLLSRYGTASLLVTGGLGVAAFTVLTLIRDRKAA"
BASE COUNT         1191 a         2214 c         2291 g         1181 t
ORIGIN      
        1 aagcgccaag ttcgtaggcg aagcgctcaa gccgcgcgtg aatatgctgc ttggccgcat
       61 cccataagag cggaccgagc ccaccgctcg catcgacgag ccgcgaatca acgacgcgca
      121 accggaggcg cgccgcatcc tcgacgtacg gcaccagcgc gaggtggatc gttcctttcc
      181 acgccgtgcc ttgacacttg cctgcgatct ctaccgcgag cgagcccgtg ccgggcgcca
      241 ctaggcgcag gcgtccctgt gcgaactcca gcttgggcgc ctgcagctcg agatagcggc
      301 agcgtccatc acggtaagga acgagctgcg cgcccaatgc ctcgcccagc gtgtcgagcg
      361 atacgcgcaa cgggatctca acgcgcgctg ccagggcgtc gtgcgcgtag ccgacgaggc
      421 aacagactgc aagaacccgc accagcatcc tacgtagtcc ggactatagc ctgttaactg
      481 tccatctccc gggctggtca gaattgatca atccggcttg tttcggctgt aaccggaagc
      541 gattgccccg aaatacggcc gagacgcgcg ctcctcacca tgaagccatg agacagcttc
      601 tccgcggcgc gcgcacgctg agcccctacc tcctcaccga ggcgctgctg cccggaggca
      661 cgctgatcgc gctcggcctt tggctctatc agaacagggg gcgcctgccg aagtggggcc
      721 gagcgagcgg caactccgat gcctcgtcga cgagcacggc actctgatcg cgctgctcct
      781 gtagctgttc cggcgtaaat tcgccgtcaa tggcgaaatc cagcaaagct tggctgcgcc
      841 ggcacatcac tgacccgtac gtaaagcagg cgcgcaagca aggctaccgc tcgagagccg
      901 ctttcaagct gctggagatc gattcaaaag agaacctgtt ccgtccaggt caaaccgtga
      961 tcgatcttgg cgccgcgcct ggcggctggt cgcaggtcag cgctgagaag atcgggcgag
     1021 gcgggcgagt gatcgccatt gacctgctgg aaatcgcccc catctccggg gtaaccgtcc
     1081 acagagggga tttccgccag attaacctgc aacttgcctt caatgcgcag gccgacgttg
     1141 tgctctccga cctctctccc agcttgtcgg gcattcccaa cgtcgaccag gcgcgctggc
     1201 tcgagctggc ggaaggagcg ctccagcttt gccgcaaggt gttgaaggct gacggtgttt
     1261 tcgtggtcaa agcctttcat ggcgaggcgt tcgacgcgct catgaggcgt atgaaggggt
     1321 ttttcagcaa ggtcaaagtt gtaaaaccct ctgcttcccg gggcgaatcg gcagagactt
     1381 acgtggtggc acgggcattg cgccccctgt aatatgggtc ttgggggtgc cgcggaataa
     1441 ggagcaagta attgaacaac atgcttaaaa acctggtcat ctggctcgtt atcgggctgg
     1501 tgctgatgac cgtgtttaac cagttcaata cccgccagtc ggctcaggcg ccgatggagt
     1561 attcccagtt cctcgaggaa gttaaggcgg gccgcatcgc caaggtcacc atcgagggcc
     1621 gccagctcaa ggccaccacc agcgaaggca agcgcgttac cagctactcg ccgggcgaca
     1681 tctggctggt gagcgatctc ctcaagtacg gcgtgaagat cgaggccaag ccggaagaag
     1741 agccttcgct cctcatgaac attttcgttt cgtggttccc gatgctgctt ctcatcggcg
     1801 tctggatctt cttcatgcgt cagatgcagg gtggcggccg tggcggcgcg ttttcgttcg
     1861 ggaagtcgcg tgcgcgcatg ctcgacgagt ccacgaacac cgtgaccttc gccgacgttg
     1921 ccggctgcga ggaagcgaag gaagaagtgt ccgagctggt cgatttcctg cgcgacccgt
     1981 ccaagtttca gaagttgggc ggccgcattc cgcgcggtgt gctgatggtc ggcaatcccg
     2041 gcaccgggaa gacgctgctc gcgcgcgcta tcgccggcga ggccaaggta ccgttcttct
     2101 cgatctcggg ttccgacttc gtcgagatgt tcgtcggcgt gggcgcggcg cgcgtgcgcg
     2161 acatgttcga gcaggcgaag aagcacgcgc cgtgcatcgt gttcatcgat gagatcgacg
     2221 cggtcggccg ccagcgcggt gcgggcctcg gcggcggcaa cgacgagcgc gagcagacct
     2281 tgaaccagct ccttgtcgag atggacggct tcgagtccac catgggcgtg atcgtcatcg
     2341 ccgcgaccaa ccggccggac gtgctcgacc cggcgctgct gcgtccgggc cgcttcgatc
     2401 gccaggtggt cgttcctctc cccgacatca gaggaagaga ggagattctc aaggtgcata
     2461 tgcgcaaggt gccgcttgcg ccggacgtga agcccgatat cctcgcgcgc ggcacgcccg
     2521 ggttctcggg cgcggacctc gccaacctgg tgaacgaggc cgcgctcttc gccgcgcgcg
     2581 gcaacaagcg cctggtggac atggacgact tcgagcgcgc caaggacaag atcatcatgg
     2641 gcgccgagcg acgttcgatg gtgatgcctg aggaggagcg gcgcaacacc gcataccacg
     2701 aatccgggca cgcgatcgtt gcgcgcctgc ttcccaagac cgacccggtg cacaaggtga
     2761 cgatcattcc gcgcggccgg gcgctcggcg tgaccatgca gctcccggag accgaccgct
     2821 acagccagga ccgcgaccgg ctcatgaaca tgatcgcggt gctcttcggc ggccgcatcg
     2881 ccgaagagat cttcatgaac cagatgacca ccggagcctc gaacgacttc gagcgcgcga
     2941 ccgagattgc gcgccgcatg gtcacgcagt ggggcatgtc cgacgagctc ggcccgatgg
     3001 tctacggcga gaacgagggc gagatcttcc tcggccgctc gatcaccacg cacaagaacg
     3061 tgtcggaggc gaccatgcag aaggtggacg ccgagatccg ccgcatcatc gaccagcagt
     3121 accgccttgc gcgcaagctc ctcgaggaca accgcgacaa ggtcgaggca atggcgaagg
     3181 cgctgctcga gctcgagacc atcgacgccg accagatcgg cgacataatg gccggcaagc
     3241 cgccgcggcc gccgaagcct tcgggcagcg cgcagtcggc gcccaagccg ccggcgggcg
     3301 agggcggtgc cgaaggggcc gcggcgccga cgcctgcagc gtaacggagt tgccacggaa
     3361 aaatctcccg gcctgcggtg cgggagattt ttctttgatc tgaagaactc gctggtgatg
     3421 ggcgtggtga acgtcacgcc cgattccttt tccgacggcg gtcgcttcct cgatcgtgcg
     3481 gccgcgatct cgcaggcgcg gcggctctcg gacgagggcg ccgatatcgt ggacatcggc
     3541 ggcgaatcga cccggccggg cgccgaccag gtctccgagg aggaggaatt gcgccgcgtc
     3601 atccccgtcc tcgacaagat tgcggacctg tgcgtctcgg tcgacacgcg ccatccccgg
     3661 gtgatgcgcg aggcgctgca ggcgggcgcg tccatgatca acgacgtcga tgcgctgcgc
     3721 gcacccggcg cgctggaggc cgtagccggc agcagttgcg gcgtgtgtct catgcacaag
     3781 aaaggcgagc ctgcgacgat gcagcgggaa ccgcactacg atgatgtcgt aggcgaggtg
     3841 aaggcttttc ttggacaacg ccttgatgcg gcgaggcgcg gcggtgttgc ggccgagcga
     3901 atcgtcgtcg atcccgggtt cggcttcggc aagacggtcg cgcacaacct tgaactgctg
     3961 cgtcgtctga acgcactcgc ggcgttgaac gcgccaatgc tcgtcggctt gtcccggaag
     4021 tcgacgctgg ggaatatcac cggccgaccc gtcgagcagc gccttgccgg gagcctcgcg
     4081 atggctttgc tcgctttaca gggtggcgca gcgatactgc gcgtgcacga cgtgaaggaa
     4141 acgaaggacg tcgtggcggt ctggcgggca taccagaacg acgcgtagcg atcgcacgcg
     4201 ctttgctagc cccgcgtggc ggcgcgacaa tagcaaaatg aagcggaaac atttcggtac
     4261 cgacggcgtt cgcggcacga taggcgagag tcccatgacg ccggatttcg tgctgcggct
     4321 gggatatgcc gccggcaagg tcatgtccgc gcaaggcggg cattctgctg tcctcatagg
     4381 caaggacacg cgcatctccg ggtacatggt cgagtctgcc ctcgaggccg ggttctcggc
     4441 ggccggcatc gacgtgcaca tgtgcggccc gatgcctacg ccagcggtcg cgtacctgac
     4501 gcgcgcgctg cgtctcgccg cgggcgtggt gatcagcgcc tcgcacaacc cgtaccccga
     4561 caacggcatc aagttcttct ccggcgacgg gttcaagctt ccggacgaca ccgaggcgag
     4621 gatcgaggtc gagatggagc agccgatggg atgcaacgag gcggcgaagc tcggcaaggt
     4681 atggcgcgtc gacgacgcgc ccgggcgcta catcgagttc tgcaaatcga cctttccaac
     4741 ccagctcgat ctaaaagggc tcaggatcgt tgtcgactgc gcgcacggcg ccgcgtacca
     4801 cgtcgcgccg cacgtgttcc atgagctagg cgccgacgtg actccgatcg gcgtggagcc
     4861 cgacggcttc aacatcaatg acgggttcgg cgccacctca cccgattgcc tcaagaaaca
     4921 cgtccttttg cagaaggcag acctgggcat tgcgctcgat ggagacggcg accgcgtcct
     4981 catggtggac gcggccgggc ggagctacga cggcgaccag cttctcttcg cgatagccaa
     5041 gcaccgctcc ggcaggcaga aagtggcggg cgtagcgggg acgctgatga ccaactacgc
     5101 cttcgagaag gcgatggcgc ggcttggaat tcccttcgcg cgcgcgaggg tcggcgaccg
     5161 ctatgtgctc gaactgatgc gcgagagagg atggcagctc ggcggcgaga actccggcca
     5221 ccttatctgc ctcgacaagc acaccacggg cgacggcatc gtgtcggcgc tgcaggtgct
     5281 cgcggccatg cgggaatcgc gccgagcgct tgccgagctc acggccgagc tcgtgatgtt
     5341 cccgcaggtg ctggtgaacg tcgcggttcc gcgcgggttc cagtgggaga agcatgaagc
     5401 gataaagaag gcgcagtccg atgtcgagcg ctcgctcgac ggcaaaggcc gtgtgctgct
     5461 tcgtccgtcg ggcaccgagc cggtgctgcg cgtcatggtc gaaggcgagc cgcgcgaggc
     5521 catagagtcg gcggcgaaga gcatcgcgga cgccgtgaag caagcggccc gctgaaaaat
     5581 cccgcccatg tagtaatcgg cctcgggctg gcgcagacgc tcgcctgggg ttcgacctac
     5641 tatctgccgg cgatcctcgc gaactccatc gccggcgagc tcggcgtcgc gacagcgtgg
     5701 gtctttaccg cattctcctg cggcctgctt ctttccgcga cccttggtcc ggcggcgggt
     5761 cgcctgatcg acgtctacgg cgggcggcgc gtcctcccgg tctccaatct cgtatttgcc
     5821 gccggccttg ctctgctcgg aagttgccaa ggcgtggctg ggatgttcgc gagctggctg
     5881 gtgatcggcg ccggcatggg cgcggggcta tacgaggcgg cattctccac gctggcggga
     5941 atctatgggc aggaggcccg ccgctccatt accggtatca ccctgatcgc cggcttcgcc
     6001 agcacggtgt gctggccgat ttccgcgtac ctggacgtca ccatcggctg gcgggcgacc
     6061 tgctacgtct gggcggcggc gcacgtcgcg ctcggcctgc cgatcaacct cctgctgccg
     6121 cggccccggc gcgcggagaa gagtggccaa tcccaggcga gggcgagctc gggccgtggc
     6181 cggctcgcca tgatggcagg cctggcgttc gtcttcgcgg cgacctggtt ttgcagcacc
     6241 gcgatggcgg cgcatctccc gcggctatta caggagtcgg gcgcaagcct ggcggccgcg
     6301 gtcgcggccg cggcgctcgt cggcccggcc caggtcgcgg cgcgtgttct ggaattctgg
     6361 ttcatgcgcc gcttccaccc aatcgtctcc gcgcgcgtcg caagcctcgc acatcccatc
     6421 ggcgccgccg ggctgatagc ggcaggctcg cccgccgcga gcggcttcgc cgtcctgcac
     6481 ggcgcgggaa acggcgtcat gaccatctcg atgggcacgc tcccgctcgc gcttttcggc
     6541 gcggcggggt acggcctgcg ccagggactg atgatcgcgc ccgcccggtt cgtttcggcg
     6601 agcgcgccct ttcttttcga cctgctcttg tcccgctatg gcacggcatc cctgcttgtg
     6661 accggcgggc tcggcgttgc ggctttcacg gtgcttacgc tcatccgcga ccggaaggct
     6721 gcttgacagg gcggcaaagg gcgcttcgcc ggctacagcg cgggcagtca cccaaggggg
     6781 tagtgaaccc ctttgcgctg gagctgctcc gaaagaaccg catcgacact cctacagcgc
     6841 actccaagag ctgggacgaa ttttcgcgcc cgggcgc
//
LOCUS       DASTPD010000251         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1764566, whole genome shotgun sequence.
ACCESSION   DASTPD010000251 DASTPD010000000
VERSION     DASTPD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764566"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFR57_08340"
     CDS             <1..509
                     /locus_tag="VFR57_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0259409.1"
                     /translation="SRFDGVRYGHRAQEHADLVDMYCRTRAEGFGNEVKRRILVGTYV
                     LSHGYYDAYYLQAQKVRRLIAQEFSRAFERCDAILGPTAPTTAFEIGAKAEDPVQMYL
                     GDIFTIPAPLAGLPAVSIPCGFDAAGLPVGLQLMGRHFSEALLLRAAHRYQQATDWHL
                     RVPTETPL"
     gene            506..1234
                     /locus_tag="VFR57_08345"
     CDS             506..1234
                     /locus_tag="VFR57_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259410.1"
                     /translation="MRLLFGIALLAVSWAAFPQNKAERHELIGKVGTRTALLTLHTGR
                     RADGSWQVAGEYVVLTTLARRFVEGESSPELGVTVLKEGTTPILFGRPPIAELRGTWR
                     DGVFKGTRYGPGGQKREQFEFSEEFPAMDSYSTDVRCEAKEGRYHAQLSLSVERGKLK
                     SLDWHAVEPGGQTCKVVASGQQPLRGGLRFVSNGCRLTLRDAGELIALSAENCSAHCG
                     AEAHLEPLLVDRRGNCELLRLGAR"
     gene            1239..2669
                     /gene="gatB"
                     /locus_tag="VFR57_08350"
     CDS             1239..2669
                     /gene="gatB"
                     /locus_tag="VFR57_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0259411.1"
                     /translation="MSWEIVVGIETHAQLLTRSKIFSGAPTAFGAPPNAQASAVDIAL
                     PGVLPVLNRVAVEHAIRFGLAVRGTINRRSIFERKNYFYPDLPKGYQISQYEVPIVQG
                     GYIEIPRKIRITRAHLEEDAGKSLHEVFHGMTGIDLNRAGTPLLEIVSEPDLRGAQEA
                     AAYARALHRLVTWIGICDGNMQEGSFRCDANVSVRRPSERLGTRCEIKNLNSFRFMEH
                     AIEFEARRQIKILEAGGTLEQETRLYDPECDETRPMRTKEEAHDYRYFPDPDLLPLVI
                     EEKMIEDLRRSLPELPEAKRARYVEKGVPAQAADLLVASRDRAEFFEQSIAAADVIAF
                     PSLYESTANAIVGDLAAYLNEQGRPFSDSSVGPQDVALIQTRVADGTISGKISKELLK
                     AKIQGERGSIDELIDKRGLRQISDAGAIEKLVDEVLAKNAKQVEDYRAGKEKAFHSLV
                     GQVMKATRGKANPAQVNEILRKKLKS"
BASE COUNT          522 a          849 c          883 g          464 t
ORIGIN      
        1 tgtcgcgctt cgatggcgtg cgctatggtc acagagcgca agagcatgcg gatctcgtcg
       61 atatgtactg ccgcacgcgc gccgagggct tcggcaacga ggtgaagcgc cgcatcctgg
      121 tcggcaccta cgtgctgtcg cacggctatt acgacgccta ctatctgcag gcgcagaagg
      181 tgcgccgtct gatcgcacag gaattttctc gagcgttcga gcgctgcgac gcgatccttg
      241 gtccaacggc gccgaccacg gcattcgaaa ttggcgcgaa agccgaggac ccagtacaga
      301 tgtacctcgg cgatatcttc accattcccg cgccgctcgc aggcctcccc gcggtgtcga
      361 ttccctgcgg ctttgatgcc gccgggttgc cggtaggcct gcagctcatg ggacggcatt
      421 tttccgaagc gctcttgctc cgcgccgcac accgttacca gcaggcgacc gactggcatt
      481 tgcgcgtgcc gacggagacg ccgttgtgag gctccttttc ggcatcgcct tgctcgccgt
      541 ttcgtgggcc gcctttccgc agaacaaggc tgaaagacac gagctcatcg gcaaagtcgg
      601 cacccggacc gcgctcctca cgctgcacac ggggcggcgc gccgacggct cctggcaggt
      661 ggccggcgag tacgtggtgc tcaccacgct ggcgcggcgc tttgtcgagg gggaaagcag
      721 cccagagctt ggcgtcacgg tcctgaagga aggcaccacg cccatcctct tcggccgacc
      781 gccgatcgcc gagctgcgcg gtacctggcg cgacggcgtt ttcaagggca cacgctatgg
      841 gcccggcgga cagaagcgcg agcaattcga gttcagcgaa gagtttcccg cgatggacag
      901 ctacagcacc gacgtgcgct gcgaggcgaa agagggccgc tatcacgccc agctctcact
      961 ttcggtggaa cgagggaagc tgaagagcct cgactggcac gcggtcgagc ccggcgggca
     1021 aacctgcaaa gtggtcgctt ccggacagca gccgctgcgc ggcggcctgc gcttcgtgtc
     1081 caacggttgt cgcttgacgc tgcgcgacgc gggcgagctc atcgcgctga gcgccgagaa
     1141 ttgctccgcg cactgcggcg ccgaggcgca tctcgagccg ctgctcgtcg accgccgggg
     1201 caactgcgag ctgctgcgcc tgggcgcgag gtgaccgagt gagttgggag atcgttgtcg
     1261 gcatcgagac gcacgcgcag cttctcaccc ggagcaagat cttctcgggc gcgcctaccg
     1321 cgttcggcgc gccgccgaac gcgcaggctt ccgcggtcga tatcgcgctt cccggggtgc
     1381 tgccggtcct caaccgggtt gcagtcgagc acgcgattcg tttcgggctc gcggtgagag
     1441 gaaccatcaa ccgtcgctcg atcttcgaac gcaagaacta cttctaccca gatctgccca
     1501 agggctacca gatcagccag tacgaagttc caatcgttca gggcggatac atcgagattc
     1561 cgaggaagat aagaatcacc cgcgcccacc tcgaggaaga cgcggggaag tcgctgcacg
     1621 aggtgttcca cggcatgacc ggcatcgacc tcaaccgcgc cgggaccccg ctgctcgaga
     1681 tcgtgtccga gcctgacttg cgcggcgcgc aggaagccgc cgcgtatgcg cgcgccctgc
     1741 accggctggt cacctggatc ggcatctgcg atggcaacat gcaggaaggc tcgttcaggt
     1801 gcgacgccaa tgtttccgtg cgtcgtccga gcgagaggct cggtacgcgc tgcgagatca
     1861 agaatctcaa ttcgttccgc ttcatggagc acgcgatcga attcgaggcg cggcgccaga
     1921 tcaagatcct ggaagccggc ggtacgttgg agcaggaaac ccgactctac gaccccgagt
     1981 gcgacgagac gcgcccgatg cgcacgaagg aagaggcgca cgattaccgt tacttcccgg
     2041 acccggacct gctacccctg gtcattgaag agaagatgat cgaggatctg cgccgctcgc
     2101 tgcccgagtt gccagaggcg aagcgcgcgc gctacgtcga gaaaggtgtt ccggcgcagg
     2161 cagccgatct gctcgtcgcc agtcgcgatc gtgccgagtt tttcgagcaa agcatagctg
     2221 ccgcggatgt aatcgctttt ccctcgctct acgagtccac ggcgaatgcg atcgtgggtg
     2281 acttggccgc atatctgaat gagcaaggtc gaccattttc ggatagttcg gtagggcccc
     2341 aggatgtcgc gctgatacag actagggtcg cagacggcac gatatcggga aagatctcga
     2401 aggaacttct aaaagcgaag attcaaggtg aaagaggctc gatcgacgag ctcatcgaca
     2461 agcgcggcct acgccagatc agcgacgccg gagcgatcga gaagctggtg gacgaggtgc
     2521 tggcgaaaaa tgccaagcag gtcgaggact accgtgccgg caaggagaag gccttccact
     2581 cgctcgtcgg acaggtgatg aaggcgaccc ggggcaaggc gaaccccgcg caggtcaacg
     2641 agattctgcg gaaaaagctg aaaagctagc gcggcttgcc gatcgcctcg cggtagcggc
     2701 gtcgatcctc gtcgtagc
//
LOCUS       DASTPD010000252         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_334905, whole genome shotgun sequence.
ACCESSION   DASTPD010000252 DASTPD010000000
VERSION     DASTPD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334905"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFR57_08355"
     CDS             <1..810
                     /locus_tag="VFR57_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0259412.1"
                     /translation="IAEKRRAFQQLHKAGCFVIPNPWDVGSARFLQSLGFKALATTSS
                     GFAWSQGRPDGGISRDMALAHLHDIVGATDLPVNADFESGFALDAAGVAESVRLAIET
                     GVAGLSIEDSTGDAAKPLYELDAAVERIRAARKAIDKAGGDVLLVGRAECFLVGRPDL
                     DETTRRLKAYAQAGADCLYAPGIHTPDQIRAVVTGVGPKPFNLLVGSATKLTMQEISA
                     LGVRRVSVGGALARSAWGGFMRAARLIAEQGKFDGFASAASGQELNSFFRQ"
     gene            complement(991..2001)
                     /locus_tag="VFR57_08360"
     CDS             complement(991..2001)
                     /locus_tag="VFR57_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU0259413.1"
                     /translation="MRQPRIQTTVVGSYPLPDWLARAPSEQALLDATRVVLHTQERAG
                     IDLVCDGELYRFDVNHPETNGMIEYFVRPMSGVRSDIGFSEWLDYSRGSGMRFRTRPP
                     GVVEGPIGPGTLNLKIPCARAKALASRALKFTVTGPHMLAKTLHDRHYKSPEKLAHAI
                     ADVLAAQVRHLDADVVQLDEANLPGHPDEWKWAASAINKVLKAVPKKAKAAVHLCFGN
                     YGGQTIQKGTWAKLIDYLNALEVDHLVMETAHRPPDELAVFKDLRKDIGFGLGVVDIK
                     RTDIEKPDDIARAIERAEKVLGKGRVKYIHPDCGFWMLPRNVADGKICALPAGRDLYL
                     GL"
     gene            2026..3036
                     /locus_tag="VFR57_08365"
     CDS             2026..3036
                     /locus_tag="VFR57_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259414.1"
                     /translation="MSHFADVEQLEEYMTRPSAELEAELSGIGDILVLGVGGKMGPTL
                     ARMAKRAAPKSRVIGVARFSDKGLREKLEHQGIECIACDLLDHAAVGKLPRAKNVVFM
                     AGHKFGATGDPSFTWAMNVAVPFMAAETFRDSRIVAFSTACVYPYVDVHGAGAPETMA
                     TTPPPGDYANSCVGREQLFLHGSRRYGTPGRLLRLSYAIDMRYGVLHDVASSVFNGNP
                     VALGMGYANVIWQGDANEHALRLLARCTIPASPINISGPKVSVRALAEEYGRRFSKKP
                     DFSGKEADTAWLIDTSQSRALLGAPRVPLGTMIDWVADWVARGQPSLGKPTHFETRDG
                     KY"
     gene            3020..3847
                     /locus_tag="VFR57_08370"
     CDS             3020..3847
                     /locus_tag="VFR57_08370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0259415.1"
                     /translation="MTESTEHPLGLEHLGGCLALSRAAHWNQNEADWRLMLTIGHGWG
                     ISVAAGVLAASTLVLPFEHFAWISMVLVLPEHRRKGYATRLLRVAIADLEKRGLAPVL
                     DATPAGREVYRLEGFRDTWGFKRYSLQDESSLETRNLARPFTEKDWKSVLELDRKAFG
                     ASRERLLRDLAHRLPRAALVHDRGYVLGREGREAFQIGPVVARDEDTAIGLVSQALSA
                     MKPPLYVDIVDHAPRLRGWLEARGFAFQRPFTRMLRGLAAAPGDEKLVYCVAGPELG"
     gene            3844..>4261
                     /locus_tag="VFR57_08375"
     CDS             3844..>4261
                     /locus_tag="VFR57_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0259416.1"
                     /translation="MTCDFIVIGAGHNGLACAAYLAKAGAKVLVVERAARVGGACHTE
                     EATLPGFKHNICSVVHTHVPLSPVYRDLELERHGVRYVYPDYLRGTIFPDHRSIVMYR
                     ETERMAAELGRYSARDANTFRQLVADYGEFIDSTYLP"
BASE COUNT          690 a         1356 c         1426 g          789 t
ORIGIN      
        1 attgccgaga agcggcgcgc ctttcaacag ttgcataagg ccgggtgctt cgtcattccg
       61 aacccctggg acgttggcag cgcgcgtttt ctacaaagtc ttggattcaa ggcgcttgcc
      121 actacaagct ctggcttcgc ctggtcgcag gggaggcccg atggtggcat ttcgcgcgac
      181 atggcgcttg cgcacctgca cgacattgtc ggggcaacag acttgccggt gaatgctgac
      241 ttcgaaagtg gcttcgcact cgatgcggct ggcgtcgcgg aaagcgttcg tcttgcaatc
      301 gagacagggg tggcgggact ttcaatagaa gattcgaccg gcgatgcggc aaaaccgctc
      361 tatgagctcg acgctgccgt cgagcggatt cgcgcggcac gaaaagcgat cgataaggcg
      421 ggcggcgatg tgctgctggt cggacgcgcc gaatgctttc ttgtcggtcg acccgatctc
      481 gacgaaacga cccggaggct caaggcctac gcgcaggctg gtgccgactg cctctacgcg
      541 ccgggtatcc acacgcccga tcagatcagg gcggtcgtga ctggcgtcgg gccgaagccg
      601 ttcaacctgc ttgtgggctc ggcgaccaag ctcacgatgc aggaaatctc tgcgctcggg
      661 gtgcggcgag tcagcgttgg gggcgcactt gcacgctcag cctggggcgg atttatgcgc
      721 gccgccagat tgattgcaga acaaggcaaa ttcgatggtt tcgccagcgc ggcctctggc
      781 caggaactca attcattctt tcgccaatga ccgtgagctc ctttcgcgaa taggcgcttc
      841 gtcctcgttc gtcaagctcg acggcggctc tgattgcttc tttccacagg gcgtttggat
      901 ggcgcggtac gttgactcgg ggagcagttg gctcgcggac ctgttcccga ctcggcgtcc
      961 ggtcgggaac ctgggcttcg attttccggg tcagagtccc aggtacaggt cgcgccccgc
     1021 gggaagggcg cagatttttc cgtcggcgac gttgcgtggc agcatccaga agccgcagtc
     1081 tgggtggatg tacttcaccc ggcctttgcc gagaactttc tcggcgcgct cgatggcgcg
     1141 cgcgatgtcg tcgggcttct cgatgtcggt gcgcttgatg tctaccacgc cgaggccgaa
     1201 gccgatgtcc ttacgcaggt ccttgaatac cgcgagctcg tcgggaggac ggtgcgcggt
     1261 ctccatcacg agatggtcga cctcgagcgc gttcaggtaa tcgatcagct tcgcccaggt
     1321 gcctttctgg atcgtctggc cgccgtagtt gccgaagcac aggtgcaccg cggctttggc
     1381 tttcttcgga acggctttca ggaccttgtt gatcgcggat gccgcccatt tccattcgtc
     1441 gggatggccc ggcaggttcg cttcgtcgag ctgcactacg tcggcgtcga ggtgcctcac
     1501 ctgcgcggcg agcacgtcgg cgatcgcgtg cgcgagcttc tccgggctct tgtagtgccg
     1561 gtcgtgcagc gtcttcgcga gcatgtgcgg cccggtaacc gtgaacttga gcgctcgcga
     1621 ggcgagcgcc ttggcgcgcg cgcacgggat cttgaggttg agcgttccgg ggccgatcgg
     1681 tccctcgacc acaccaggcg gccgtgtgcg aaagcgcatg cccgagccgc gcgagtagtc
     1741 gagccattcc gagaagccga tgtcgctgcg cacgcccgac atcggccgca cgaaatactc
     1801 gatcatgccg ttggtttcgg gatggttgac gtcgaagcgg tagagctcgc catcgcacac
     1861 gagatcgatt cccgcccgct cctgcgtgtg cagcacgacg cgggttgcat cgaggagcgc
     1921 ctgttccgac ggcgcgcgcg cgagccagtc gggcagcgga tacgagccga ccaccgtggt
     1981 ctggatgcgc ggctgtctca tttcttgaaa ttatagaatt cggccatgag ccacttcgcc
     2041 gatgtcgagc agctcgaaga gtacatgacg cggccgtcgg cggagctcga ggccgagctc
     2101 tcgggcatcg gcgacatcct cgttctcggc gtcggcggca agatgggccc gacgctcgcg
     2161 cgcatggcga agcgcgccgc gccgaagagc cgcgtgatcg gcgtcgcccg cttttccgac
     2221 aagggcctgc gcgagaagct ggagcaccag gggatcgaat gcatcgcctg cgatcttctc
     2281 gaccacgccg cggtcggaaa gctgccgcgc gcgaagaacg tggtcttcat ggctggccac
     2341 aagttcggcg cgacgggcga tccctcgttc acctgggcca tgaacgtcgc ggtgccgttc
     2401 atggccgccg agacgttccg cgattcgcgc atcgtcgcgt tttccacggc ttgcgtttat
     2461 ccatacgtgg atgtgcatgg cgccggggcg ccggaaacga tggccaccac gccgccgccc
     2521 ggcgactacg ccaactcgtg cgtcgggcgc gagcagctgt tcctgcacgg ctcgcggcgc
     2581 tacggcacgc ctgggcggct cctgcggctt tcgtacgcga tcgatatgcg ctacggggtg
     2641 ctgcacgatg tcgcctccag cgtcttcaac ggcaacccgg tcgcgctcgg catggggtac
     2701 gcgaacgtca tctggcaggg cgacgccaat gagcacgcgc tgcggctcct cgcccgctgc
     2761 accataccgg cgagcccgat caacatcagc ggtcccaagg tgagcgtgcg cgcgctggcg
     2821 gaggagtacg gccggcgctt ctccaagaag ccggacttct cgggcaagga agcggatact
     2881 gcgtggctga tcgacacttc gcagtcgagg gcgctcctcg gcgcgccgcg cgtgccgctc
     2941 ggcacgatga tcgactgggt cgcggactgg gtcgcccgag gccagccgag cctaggcaag
     3001 cccacgcatt tcgagacgcg tgacggaaag tactgagcac ccgctcgggc tcgagcacct
     3061 gggcggctgc ctcgcgcttt cccgggcagc gcactggaac cagaacgagg ccgactggcg
     3121 gctcatgctt acgatcggcc acggctgggg catcagcgtc gcggccggcg tgctcgccgc
     3181 ttcgacgctc gtgcttccct tcgagcactt cgcctggatc agcatggtgc tcgtactgcc
     3241 ggagcaccgg agaaagggct atgcgacgcg gctacttcga gtggcgatcg ccgacctcga
     3301 gaagcgaggt ctcgccccgg tgctcgatgc caccccggcc gggcgggagg tctaccgtct
     3361 cgaaggcttt cgcgacacct gggggttcaa acgctactct ttgcaagacg aatcctcctt
     3421 ggaaactcgg aatttggccc gcccgttcac tgagaaagac tggaagagcg tattggagct
     3481 ggaccgcaag gcctttggcg cgagccgcga gcggctgttg cgcgacctgg cacatcggct
     3541 tccacgggcg gcgctcgtac acgaccgcgg ttatgtgctc ggccgcgaag gaagggaagc
     3601 gttccagatt ggcccagtgg tcgcgcgcga cgaggacacc gcgatcgggc tcgtctcgca
     3661 ggcgctaagc gcaatgaagc cgccgctcta cgtcgatatc gtcgaccacg cgccgcggct
     3721 gcgcggctgg ctggaagcgc gcggcttcgc cttccagagg cccttcacgc gcatgctgcg
     3781 cggcctggcc gccgcgccgg gagatgaaaa gctggtctac tgcgtggcag gaccggagct
     3841 cgggtgacct gcgacttcat cgtcatcggc gcgggccaca acggccttgc ctgtgccgcc
     3901 tatctcgcga aggccggcgc caaggtcctc gtcgtggagc gcgccgcgcg cgtgggcggg
     3961 gcctgccata ccgaagaagc gacgctacct ggcttcaagc acaacatctg ctcggtggtg
     4021 catacgcatg ttccgctcag cccggtctat cgggacctag agctcgagcg tcatggcgtg
     4081 aggtacgtgt acccggacta cctgcgcggc acgatcttcc cggatcacag gagcatcgtg
     4141 atgtaccgcg aaaccgagcg catggccgcc gagctcggcc ggtactcggc gcgcgatgcg
     4201 aacaccttcc gccagctcgt tgcggactac ggggagttca tcgactcgac ctacctgccg
     4261 c
//
LOCUS       DASTPD010000253         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1153049, whole genome shotgun sequence.
ACCESSION   DASTPD010000253 DASTPD010000000
VERSION     DASTPD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153049"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFR57_08380"
     CDS             <1..526
                     /locus_tag="VFR57_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259417.1"
                     /translation="PVVMGCSIGGRIVLDLALKYARELRALIGLQSGAYVERYYDASW
                     LDHPEVHGGRACGAVAYGLMSPLSPEKERWETVWHYMQGGPGVFTGDLYFYQVEGDIR
                     GRIGDIDTSICPLYLLTGEYDYSASPRDTEAVVARVKGAKMTVMKGLGHFPMSENPDA
                     FVGYLRPVLDEIRT"
     gene            523..1953
                     /locus_tag="VFR57_08385"
     CDS             523..1953
                     /locus_tag="VFR57_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0259418.1"
                     /translation="MKRLLVALATAVSLGHAQADEALLEAMAQPWTGDLDGMIERRTV
                     RALVVPSRMQYWIDRGRQSGAEYELLKKFEEELNAKYKLKRKHIGIHVHFIPTARDEL
                     IPALVQGRGDIAAGILTITPERLQQVDFGAPFFRNVAEIVVTGPASPTLASIEDLAAQ
                     KLFVRRSSSYWTHLEALSERLRQQGRASLELKAAPEDLQDDDLLEMLNAGLVGVAVVD
                     RYKAVTWARVFKRIKPREDLKVHEGGDIAWMIRKDSPNLKAEISAFAQKHGQDSAFGK
                     NLVKKYTGSTRFVRAATSKEEMRKFDQTAEIFRKYSGRYDMDYLLVMAQGYQESRLDQ
                     NAKSPVGAIGVMQVMPATGRDMKTGDITQLDPNIHAGVKYIRYVQDTFFENQPMDAVN
                     KTLFSFAAYNAGPGRVQELRARAEKRGLNPNVWFNNVELIAAERIGAETVTYVSNIYK
                     YYVAYKLAVEIRAERDRERDALKKKP"
     gene            1974..>2291
                     /locus_tag="VFR57_08390"
     CDS             1974..>2291
                     /locus_tag="VFR57_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259419.1"
                     /translation="MLGGAAAIGAAPGAAQAQATAVPRSGWSFEFTPYLWGAAMEGSV
                     GAGELPTMNIDMSFSDVLDHLDAGLMGAFEARKGRWGALLDAIYMKIEDSATATRTGA
                     GPVG"
BASE COUNT          439 a          722 c          778 g          352 t
ORIGIN      
        1 cccggtggtg atgggctgct cgattggcgg gcgcatcgtg ctcgacctcg cgctcaagta
       61 tgcgcgcgag ctgcgcgcgc tgatcgggtt gcagagcggc gcctacgtgg agcgctacta
      121 cgacgcttca tggctcgacc atcccgaagt gcatggcggc cgcgcctgcg gcgcggtagc
      181 ttatgggctt atgtcgccgc tcagcccgga gaaagagcgg tgggagaccg tgtggcacta
      241 catgcagggc ggccctggcg tgttcacggg ggacctctac ttctaccagg tggaaggcga
      301 tatccgtggc cgcatcggcg atatcgacac cagcatctgc ccgctttatc tgctcaccgg
      361 cgaatacgac tactcggctt cgccgcgcga tacggaagct gtcgtggcgc gggtgaaagg
      421 ggcgaagatg acggtcatga aaggcctcgg ccatttcccc atgagcgaga accccgacgc
      481 gttcgtaggt tacctgcgac cggtgcttga cgagatccgc acatgaagcg cctgctggtc
      541 gcactggcaa cggcggtcag cctcggccat gcgcaggccg acgaggcgct gttggaggcg
      601 atggcacagc catggacggg ggacctcgac ggcatgatcg agcgccggac ggtgcgcgcg
      661 ctggtggtgc cttcccgcat gcagtactgg atcgaccgcg ggcgccagag cggcgcagag
      721 tacgagctcc tgaagaagtt cgaggaggag ctcaatgcga agtacaagct caagcgcaag
      781 cacatcggca tccacgtgca cttcatcccg accgcgcggg acgagctcat ccctgcgctg
      841 gtgcaaggcc gcggcgacat cgccgccggc atcctcacca ttacgcccga gcggctgcag
      901 caggtcgact ttggcgcgcc gtttttccgc aacgtcgccg aaatcgtagt gaccggcccc
      961 gcttcgccaa cgcttgcgtc gatcgaggat ctcgcggcgc agaagctctt cgtgcgccgc
     1021 tcgtcgagct actggacgca cctcgaagcg ctcagcgagc gcttgcgcca gcagggccgc
     1081 gcatccctcg agctgaaagc ggcgcccgag gacttgcagg acgacgatct gctcgagatg
     1141 ctgaacgcgg gcctggtggg tgtcgcggtc gtcgaccgct acaaggccgt cacctgggcg
     1201 cgcgttttca agcgcatcaa gccgcgcgag gacctgaagg tccacgaggg cggggacatc
     1261 gcatggatga tccgcaaaga cagcccgaac ctcaaagccg agatctccgc gtttgcgcag
     1321 aagcacggac aggacagcgc cttcggcaag aacctggtga agaagtacac cggcagcacg
     1381 cgcttcgtcc gcgccgcgac gtcgaaagag gagatgcgca agttcgacca gacggcggag
     1441 atcttccgca agtacagcgg gcgctacgac atggactacc tcctggtgat ggcgcagggc
     1501 taccaggagt cgcgcctcga tcagaacgca aagagcccgg tcggcgcgat cggcgtgatg
     1561 caggtgatgc cggccacggg ccgggacatg aagacggggg acattaccca gctcgacccg
     1621 aacatccacg ccggggtcaa atacatacgc tacgtgcagg acaccttctt cgagaaccag
     1681 cccatggatg cggtcaataa gacgctcttc tcgttcgctg cctacaacgc cgggcccggc
     1741 cgggtgcagg agctgcgcgc gcgcgccgag aagcgcgggc tgaatccgaa cgtctggttc
     1801 aacaacgtcg agctgatcgc cgccgagagg atcggcgcgg aaaccgtgac ctacgtttca
     1861 aacatctaca agtattacgt tgcctacaag ctcgcggtcg agatccgggc cgagcgcgac
     1921 cgggagcgcg acgcactgaa gaagaaaccc tgaaaatcgc cgcgatcgcc gtcttgctgg
     1981 ggggcgccgc cgcgatcggt gcggcgccgg gcgccgcaca agcgcaagcc acggcggtgc
     2041 cgcgcagcgg ctggagcttc gaattcacgc cttacctctg gggcgcggcg atggaaggaa
     2101 gcgtcggggc gggagaactg ccgacgatga atatcgacat gagcttctcc gacgtcctcg
     2161 accacctgga cgcggggctg atgggggcgt tcgaggcgcg caaggggcgc tggggggcgc
     2221 tgctcgacgc gatctacatg aagatcgaag actcggcgac ggccacccgc accggcgccg
     2281 gtcccgtcgg c
//
LOCUS       DASTPD010000254         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1561067, whole genome shotgun sequence.
ACCESSION   DASTPD010000254 DASTPD010000000
VERSION     DASTPD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561067"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..437)
                     /locus_tag="VFR57_08395"
     CDS             complement(171..437)
                     /locus_tag="VFR57_08395"
                     /inference="COORDINATES: protein motif:HMM:NF023369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEU0259420.1"
                     /translation="MDSDRPTLMYESDITKFLRQYLEEHPEEVESQRKGRAVWWDKTP
                     DERSPTPSMQHAPRAGGNEHTFEPAGGYEWSFGVDDNAEPGDKE"
     gene            complement(413..1564)
                     /gene="prpC"
                     /locus_tag="VFR57_08400"
     CDS             complement(413..1564)
                     /gene="prpC"
                     /locus_tag="VFR57_08400"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEU0259421.1"
                     /translation="MSSSPAAAPKPKKSVALSGVPAGNTALCTVGRSGNDLHYRGYDI
                     LDFAEQAEFEEVAYLLVHEKLPTRDELRKYKSSLRRMRGLPKALKGALERVPKRAHPM
                     DVLRTGVSVLGTIEPEKDDHGKAGAQRIADRLMASMGSILLYWYHYSHRGKRIETETD
                     DDSIGGHFLRLLHNKKPSEAAVRAMHTSLILYAEHEFNASTFACRVVAGTGSDLYSAI
                     CAGIGALRGPKHGGANEFAFEVLSRYKNPDEAEADIVRRLQAREIVIGFGHPVYTTGD
                     PRNQVIKGVAKRLSQQAKSMKLFDIAERLEHVMRREKDMFANLDWYSAVSYHRLGVPT
                     AMFTPVFVISRTSGWSAHIIEQRADGKIIRPGANYTGPDNLQWIPIDQR"
     gene            complement(1536..2414)
                     /gene="prpB"
                     /locus_tag="VFR57_08405"
     CDS             complement(1536..2414)
                     /gene="prpB"
                     /locus_tag="VFR57_08405"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEU0259422.1"
                     /translation="MSAGKRFREALSQEKPLQIVGTINAYHALMAKRAGYRAIYLSGG
                     GVAAGSLGMPDLGISNLDDVLTDIRRITDVCDLPLLVDVDTGFGASAFNVARTTRSLI
                     KFGAAAMHIEDQVGAKRCGHRPGKELVSKDEMADRIKAAVDARTDSGFVIMARTDALA
                     NEGLESALSRAISYVEAGADMIFPEAITDLPMYKRFAEAVKVPILANITEFGKSPLFT
                     VEELRSANVAMILYPLTAFRAMNKAALRAYEQLRRTGTQKALIADMQTREELYDLLNY
                     HSYEKKLDELFSRGRS"
     gene            complement(2411..>2600)
                     /locus_tag="VFR57_08410"
     CDS             complement(2411..>2600)
                     /locus_tag="VFR57_08410"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399917.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU0259423.1"
                     /translation="GIPSDGSYGIPEGVIFGYPVTCRGGQYQIVKGLEISEFSRKRID
                     ATLKELHEERDSVKTLLS"
BASE COUNT          417 a          815 c          866 g          502 t
ORIGIN      
        1 tagggccgcc gcggcgagca ggaagtaccc gagcatctcg aaaagctcgg tgacccagcc
       61 gggccacgaa gtgggcggcg cgctaaagac gaccagtagc agcaccgcca ggatcgcggt
      121 gcgcgcccag acgatgcggt ggctggcgaa atgctcgaac atgcggatcc tcattccttg
      181 tcgccgggct cggcgttgtc gtcgacgccg aagctccact cgtagccgcc ggccggctcg
      241 aaggtgtgct cgttgccgcc cgcgcgcggg gcgtgctgca tggacggggt gggggagcgc
      301 tcgtccggag tcttgtccca ccagacggcg cgacccttcc tttgcgactc gacctcttcg
      361 ggatgctcct cgagatactg gcgcaggaac ttcgtgatgt cggattcgta catcagcgtt
      421 ggtcgatcgg aatccattgc aggttatcgg gcccggtgta attggcgccg ggacggatga
      481 ttttgccgtc ggcgcgctgc tcgatgatgt gcgccgacca gccgctggtg cgcgagatca
      541 cgaacaccgg agtgaacatt gccgtcggca cgccgagacg gtggtaggac accgcgctgt
      601 accagtccag gttggcgaac atgtccttct cccgccgcat cacgtgctcg agccgctcgg
      661 cgatgtcgaa cagcttcatg ctcttcgcct gttgcgaaag acgcttcgcc acgcccttga
      721 tcacctggtt gcgcggatcg ccggtcgtgt acacggggtg gccgaagccg atcacgatct
      781 cgcgcgcctg caggcggcga acgatgtcgg cttcggcctc atccggattc ttgtatctcg
      841 acaagacctc gaaggcgaac tcgttggcgc cgccgtgctt cgggccgcgc agcgctccga
      901 tgccggcgca gatcgcgctg tataggtcgg agcccgtgcc cgcgaccacg cggcaggcga
      961 aggtgctggc gttgaactcg tgctcggcgt acaggatgag cgacgtgtgc atggcccgga
     1021 ccgccgcctc gcttggtttc ttgttgtgca gaagcctcag gaagtggccg ccgatggagt
     1081 catcgtcggt ctcggtttcg atgcgcttgc cgcggtggct gtagtggtac cagtagagca
     1141 ggatcgagcc catagaggcc atcagccggt cggcgatgcg ctgcgcgccc gccttgccgt
     1201 ggtcgtcctt ctccggttcg atcgtgccga gcaccgaaac cccggtgcgc agcacgtcca
     1261 tcggatgcgc cctcttcggc acgcgctcga gcgccccctt gagcgccttg ggtagtccgc
     1321 gcattcttct gagcgagctc ttgtatttcc tcagctcgtc gcgggtcggc agcttctcgt
     1381 gcacgagaag gtaggcgacc tcctcgaact cggcctgctc ggcgaaatcg aggatgtcgt
     1441 agccgcggta gtgcaggtcg ttgccgctcc ggccgacggt gcaaagcgcc gtattgccgg
     1501 ccggaacgcc ggaaagggcg accgattttt tcggcttagg agcggccgcg ggagaagagc
     1561 tcatcgagct ttttctcgta ggagtggtag ttgaggaggt cgtagagctc ttcgcgcgtc
     1621 tgcatgtccg cgatcagcgc cttctgcgta ccggttctgc gaagctgctc ataggcgcgc
     1681 agcgccgcct tgttcatcgc ccggaacgcg gtgaggggat agaggatcat ggcaacgttc
     1741 gccgagcgca gctcctcgac cgtgaaaagc ggcgactttc cgaactctgt gatgttggcg
     1801 aggatcggca ctttcacggc ttccgcgaag cgcttgtaca tcggcagatc ggtgatcgcc
     1861 tcgggaaaga tcatgtccgc ccccgcctcc acgtaagaaa ttgcacgcga aagggcagat
     1921 tccaaaccct cgttggccag cgcgtcggtg cgcgccatga tcacgaaccc cgagtcggtg
     1981 cgcgcatcca ccgccgcctt gatgcggtcg gccatctcgt ctttggagac gagctccttc
     2041 ccggggcggt ggccgcagcg cttcgccccg acctggtcct cgatgtgcat cgccgcggcg
     2101 ccgaacttga tgagcgagcg cgtagtgcgc gcgacgttga acgcgctcgc gccgaagccg
     2161 gtatcgacgt cgacgagaag cggcaggtcg cacacgtccg tgatccggcg gatgtcggtg
     2221 agtacgtcgt cgaggttcga gatgccgagg tccggcatgc cgagcgagcc ggcggccacg
     2281 ccgccgcccg agagataaat ggcgcgatat cccgcgcgct ttgccatcaa cgcgtggtat
     2341 gcgttgatcg tgccgacgat ctgaaggggc ttttcttggg agagggcctc gcggaaccgc
     2401 ttgcccgcgc tcatgacaga agggttttca cgctgtcgcg ctcttcgtgc agctccttca
     2461 gcgtagcgtc gatgcgcttg cggctgaatt ccgaaatctc gaggcccttc acgatctgat
     2521 attggccgcc gcggcaggtg accgggtagc cgaagatcac gccctccgga atgccgtagc
     2581 tgccgtccga agggatgccc
//
LOCUS       DASTPD010000255         9484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1561350, whole genome shotgun sequence.
ACCESSION   DASTPD010000255 DASTPD010000000
VERSION     DASTPD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561350"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VFR57_08415"
     CDS             complement(<1..922)
                     /locus_tag="VFR57_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0259424.1"
                     /translation="MPDRYEDLYRSYRWEVPARYNIARACCGSWAGDRTRFALYWEDE
                     SGAMAAYSFWDIQQAANRLSNALAALGVNRGDRVAIILPQRPETAIAYMAVFQMGAVA
                     LPLSHLFGPEALEYRMEHAEASIAIVEPSTIANLWAIRDRLTHLRHVIGVGGARERNV
                     QPWEDLVAKAASRFSPVDTAADDPALIIYTSGTTGPPKGALKAHRVLIGNVPGFVHSH
                     DFFPKPGDMFWSPADWAWTGGLMDALLPAWMFGIPILGYRGRFDPEKAYYLLEKYGVR
                     NSFLFPTALKLMMKAFPSPKAKFDISLRSIM"
     gene            946..1584
                     /locus_tag="VFR57_08420"
     CDS             946..1584
                     /locus_tag="VFR57_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0259425.1"
                     /translation="MAAEAFVFDAYGTLYDVHSVAARCESYWPGKGASVSQLWRAKQL
                     EYTWQRSLMRRYEPFSSVTRAALSYACAAFRLELEGAQVDALMEEYTRLALYPEARAA
                     LSRLRGKKAILSNGSPDMLLPLVENSALPFDAVMSVDEVKVFKPAPEVYELAVRRLRT
                     ESVAFISSNCWDALGAKSYGFTVYWINRARAPIDRLGFEPDAVLTSLDEVLQ"
     gene            1568..2095
                     /locus_tag="VFR57_08425"
     CDS             1568..2095
                     /locus_tag="VFR57_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0259426.1"
                     /translation="MKFCSNCAAAVVKRVPPGDTLPRWVCDHCGEIHYQNPKLVVGSI
                     PEHDGKVLLCRRAIEPRYGYWTLPAGFMENDETAAEAALRETLEEAGTRVELGAPFTM
                     ISVPHVNQVHLFYRARVLGLEFKPGEESLEVALWDERQIPWKEIAFRTVGLTLKHWFA
                     DRGRGAFGFHAEDLK"
     gene            2122..3405
                     /locus_tag="VFR57_08430"
     CDS             2122..3405
                     /locus_tag="VFR57_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0259427.1"
                     /translation="MKKVLPWLLVIAAPAVLAQAPATPKIDNANTAWMLVSAALVLFM
                     TLPGLALFYAGLVRAKNVLSVLMQCFSITCVVTLAWFAVGYSIAFGDANAWWGGVGRA
                     FFSGMQVATVKGTIPETLFAMYQLTFAIITPALVIGAYAERVRFGAMLLFSVLWLLIV
                     YCPLAHWVWGDGWLQKRGVMDFAGGLVVHLNAGVAALVCALVLGRRRGFPETQMAPHN
                     MTMCATGAGMLWVGWFGFNAGSALAADGSAAMAMLVTHIAAATGAFVWMLCEWVRYGK
                     PSALGIVTGMVAGLGTITPASGFVGPIGALVIGAAAGLVCFFATNYMKRALNIDDSLD
                     VFPVHGVGGLMGTLLTGIFVSSAFNGIGYPEKVTMGEQFVTQLVGVVATAVWSGALTW
                     ILLKLVDAIAGMRVAADEETEGLDTVLHNEKGYNL"
     gene            3409..4098
                     /locus_tag="VFR57_08435"
     CDS             3409..4098
                     /locus_tag="VFR57_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0259428.1"
                     /translation="MERRGFIKFCAASVAALKAGEVAADARVQPYARAKLMDEKGAPL
                     RAGSIPADRNLIFHYPYAATPCFLLNLGKPVRPARLKTHSGEAYEWKGGVGAQRSIVA
                     YSAICAHRLSYPTKDISFISYRTEKSARNRIANVIHCCSEHSQYDPAEGARVVAGPAP
                     QPLAAIVLEWSQATDEIEAVGTLGGEMFREFFEKFGFKLEIEHGAPRATVAGTCVVQE
                     LDNYCKQQVKC"
     gene            4092..4766
                     /gene="aat"
                     /locus_tag="VFR57_08440"
     CDS             4092..4766
                     /gene="aat"
                     /locus_tag="VFR57_08440"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU0259429.1"
                     /translation="MLAWLQGKDPFPPIERALKNPNGLLAAGGALTVERLLEAYRHGV
                     FPWYSGSEPILWWSPDPRMVLYCDELKVSRSLAKNIRNRGYEVRVDSAFAAVLDGCAE
                     RPQTWLGPDMKKAYVRLHRAGHAHSFETWRDGEMVGGLYGVAIGRMFYGESMFSRATD
                     ASKVALVSLVAELRARRFPLIDCQMHTPLLASLGAREIPRRAFLRELTALVHYPQQPG
                     KWNVKT"
     gene            4753..5484
                     /locus_tag="VFR57_08445"
     CDS             4753..5484
                     /locus_tag="VFR57_08445"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEU0259430.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPVGQFNTNRSQRRAWKQHYALTAHP
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLIEFRDAGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRNASFGTYNVLWQIEQCRSLALPYLYLGYWIRESPK
                     MAYKSKFEPIEGFVGGAWRRLGAKV"
     gene            5481..6473
                     /locus_tag="VFR57_08450"
     CDS             5481..6473
                     /locus_tag="VFR57_08450"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU0259431.1"
                     /translation="MSLYGRARPLLFSLDPETAHRLGLRLAALVPRATFRAPVKALGL
                     EFPNPVGLAAGLDKDAAHIDALARLGFGFIEVGTVTPQAQPGNPKPRLFRLPQAQALI
                     NRFGFNNVGVDEFLANVRRTRWRGILGINIGKNATTPAERAVEDYEQCLAKVYAAASY
                     ITINISSPNTAGLRALQEKDSLEHLLARLVSLRQRMIVQHGKHVPLVLKVAPDLDADD
                     LRAIAEAVKRHGIEAVIATNTSTSREGVERLPHAAEAGGLSGAPILKKSTAVLRELAR
                     SLPQTTLIGAGGILSGADAQQKFAAGASLVQLYTGLIYRGPSLVGECVSAYRAK"
     gene            6593..6781
                     /locus_tag="VFR57_08455"
                     /pseudo
     CDS             6593..6781
                     /locus_tag="VFR57_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            6919..7377
                     /locus_tag="VFR57_08460"
     CDS             6919..7377
                     /locus_tag="VFR57_08460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0259432.1"
                     /translation="MSVVRAYREDDLDAVAAIHSVSFPRQLRSRVWIAASFGAYPKTR
                     MYVAESDARVIGFAMWTEKSGFRAHAVLELEQIAVAPEHRRRGIAEALIRRSLSEVEK
                     DLETLKAVVVTTRADNAAQALYRKTLGAEVEATIASLYSGDEVLMVARLR"
     gene            7374..8048
                     /gene="nth"
                     /locus_tag="VFR57_08465"
     CDS             7374..8048
                     /gene="nth"
                     /locus_tag="VFR57_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0259433.1"
                     /translation="MNPQKRLEIFRRLHAANPKPTTELRYTTPYELLVAVVLSAQATD
                     KSVNLATAELFRKANTPEKMVKLGVAGLEEYIKRIGLYRMKAKNVIALSQILLERHCG
                     AVPQNREALEALPGVGRKTANVVLNTAFGQPTVAVDTHIFRVSNRTGIAPGKDPFEVE
                     LKLLKFTPPEYLKNAHHWLILHGRYVCLARKPGCPQCLIRDLCEFRPKTKDVPADPRP
                     GKRHVL"
     gene            8005..8430
                     /locus_tag="VFR57_08470"
     CDS             8005..8430
                     /locus_tag="VFR57_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEU0259434.1"
                     /translation="MFQPTRDQARDTFFEAWRKYRAGQPVVGIEALALEVILQHPEYH
                     AALSDPERTRQREYVDESNPFLHMSLHVALEEQLSIDQPPGIVARFQALVHRYGERHD
                     ALHHALECLAEMVWRSQRDQAPPDARAYLDCLAARALRK"
     gene            8545..9150
                     /locus_tag="VFR57_08475"
     CDS             8545..9150
                     /locus_tag="VFR57_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0259435.1"
                     /translation="MKERSTIPALFMAACAILAIAWAAPARPAGLDDSIILIAKRQLQ
                     DKLYSSTILLARPIGGGRHVGFIINKPTQVTLGKLFPTHEPSRKVTDPVFLGGPVSPE
                     VIFALVQGKSSPGGRSIRILDDLYLAIDSDVVDRVIEKQSSHARFFAGMVLWRPGELE
                     DEVKKGLWYMQDARADLVLRKPTDSMYEELVGRSERKANAI"
     gene            complement(9287..>9484)
                     /locus_tag="VFR57_08480"
     CDS             complement(9287..>9484)
                     /locus_tag="VFR57_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259436.1"
                     /translation="SDPTQPILAGQYYDYLVRAITDYKNGRRNNPIMKGFAAQLSKQD
                     IEDLAAWFSSQKSNLHEQRKD"
BASE COUNT         1655 a         3169 c         3005 g         1655 t
ORIGIN      
        1 tcatgatcga gcgcagcgaa atatcgaact tcgctttcgg cgaaggaaag gctttcatca
       61 tcagcttgag ggcggtcggg aagaggaacg aattgcgcac gccgtacttc tcgagaaggt
      121 agtacgcctt ttcggggtcg aagcggccgc ggtagccgag gatcgggatg ccgaacatcc
      181 acgcaggcag cagcgcgtcc atgagcccgc cggtccaggc ccagtcggcc ggcgaccaga
      241 acatgtctcc cggcttcgga aaaaagtcgt gcgaatgcac gaaacccggc acgtttccga
      301 tcagcacgcg atgcgccttg agcgcgccct tcggtgggcc ggtcgtgccg ctcgtatata
      361 tgatgagcgc cggatcgtcc gccgcggtgt ccacggggga aaaacgcgac gccgctttgg
      421 cgaccaggtc ctcccagggc tgaacattgc gctcacgcgc accgcccacg ccgatcacgt
      481 gacgcagatg cgtgagccga tcgcggatcg cccagaggtt cgcgatcgtg ctcggctcca
      541 cgatcgcgat cgacgcttcg gcgtgctcca tgcggtactc gagcgcttcg ggcccgaaga
      601 gatgcgaaag cggaagcgca accgcgccca tctggaacac cgccatatac gcgatcgccg
      661 tttccgggcg ttgtggaagg atgatggcga cgcggtcgcc gcggtttacg ccgagcgcgg
      721 caagcgcatt ggaaagccgg ttcgccgcct gctggatatc ccagaacgag tacgccgcca
      781 ttgcgccgga ctcgtcttcc cagtagagcg cgaagcgcgt gcggtccccc gcccagctcc
      841 cacagcacgc gcgcgcgatg ttgtagcgcg cgggcacctc ccaacgatag ctccgataga
      901 gatcctcgta gcggtcgggc atgcggagat tctatactcc ggcgcatggc ggccgaggcc
      961 ttcgtcttcg acgcgtacgg cacgctctac gatgtgcact cggtggccgc gcgctgcgaa
     1021 tcgtactggc cgggaaaagg cgcgtcggtc tcccagctct ggcgcgcgaa gcaactcgag
     1081 tacacgtggc agcgcagcct catgcgccgc tacgagccgt tctccagcgt gacacgcgcc
     1141 gcgctttcgt atgcctgcgc ggcgttcaga ctggagctcg aaggagcgca ggtcgatgca
     1201 ttgatggagg agtacacccg gctcgcgctg tacccggagg cgcgagcagc gctttcgcgg
     1261 ctaaggggaa agaaggcgat cctgtccaac ggctcgcccg acatgctgct tccgctcgtt
     1321 gaaaactcgg cgctgccttt cgacgcggtg atgagcgtcg acgaggtgaa agtcttcaag
     1381 cccgcgccgg aggtatacga gctcgctgtg cgccggctca ggaccgaaag cgtcgcgttc
     1441 atttcctcca attgctggga cgcgctcggc gccaagtcct atgggtttac cgtctactgg
     1501 ataaatcgcg cgcgggcacc aatcgaccgc ctgggattcg aacctgacgc tgtgctgacg
     1561 tcgctcgatg aagttctgca gtaattgcgc ggctgcggtg gtgaagcgcg tgccgcctgg
     1621 cgacaccctg ccgcgctggg tctgcgacca ctgcggcgag atccactacc agaaccccaa
     1681 actcgtcgtc ggctcgatcc ccgaacacga cggcaaagtg ctgctctgcc gccgtgccat
     1741 cgagccgcgc tacggctact ggacgctgcc cgcgggattc atggagaacg acgaaaccgc
     1801 ggccgaggcg gcgctgcgcg agacgctcga ggaagccggc acgcgcgtgg agctcggtgc
     1861 gccgttcacc atgatttcgg tgccgcatgt gaaccaggtg catctcttct accgggctcg
     1921 cgtgctggga ctcgaattca agcccggcga ggaaagcctc gaggtcgcgc tttgggacga
     1981 gcgccagatt ccctggaagg aaatcgcctt ccgcaccgtc gggctcacgc tcaagcactg
     2041 gttcgccgac cgcggccgcg gcgctttcgg cttccatgca gaggacctaa aataaatcgc
     2101 aacaacaggg ggaggtccca gatgaagaaa gttctgccgt ggctgctcgt gatcgccgcg
     2161 cctgccgttc tcgcccaggc gccggcgacg ccgaaaatcg acaacgccaa caccgcctgg
     2221 atgctcgtct cggcggcgct cgtgctcttc atgacgctgc cggggctcgc gctgttctac
     2281 gccggtctgg tgcgcgcgaa gaacgtgctc tcggtcctca tgcagtgctt ctccatcacc
     2341 tgcgtagtga cgctcgcctg gttcgccgtc ggctacagca tcgccttcgg cgacgcgaac
     2401 gcctggtggg gcggggtcgg cagggcgttc ttttccggaa tgcaggtcgc gacggtaaag
     2461 ggcacgatcc cggagacgct gttcgcgatg taccagctca ccttcgccat catcaccccg
     2521 gcgctcgtca tcggcgcgta cgccgaacgg gtgcgcttcg gcgcaatgct acttttcagc
     2581 gtgctgtggc ttctcatcgt gtattgcccg ctcgcgcatt gggtatgggg cgacggctgg
     2641 ctgcaaaagc gcggcgtcat ggatttcgcc ggcgggctgg tggtccatct caacgccggc
     2701 gtcgccgcgc tcgtgtgtgc gctcgttctc ggccgccgcc gcggcttccc ggaaacgcag
     2761 atggcgccgc acaacatgac catgtgcgcc accggcgcgg ggatgctctg ggtcggctgg
     2821 ttcggcttca acgcgggcag cgccctcgcc gccgacggct cggccgccat ggcgatgctg
     2881 gtcactcaca tcgccgcggc caccggagcg ttcgtctgga tgctctgcga gtgggtgcgc
     2941 tacggcaaac cctcggcgct cggcatcgtc acggggatgg tcgcgggtct gggcacgatt
     3001 acccccgcct ccggcttcgt cgggccgatc ggcgcgctcg tcattggtgc ggcggcgggc
     3061 ctcgtctgct tcttcgccac caactacatg aagcgcgcgc tcaacatcga cgactcgctc
     3121 gacgtcttcc ccgtgcacgg ggtgggcggt ctcatgggca cgctgctgac ggggatcttc
     3181 gtatcgagcg ccttcaacgg catcgggtac ccggaaaagg tgacgatggg cgagcagttc
     3241 gtgacccagc tcgtcggcgt cgtcgccacc gcggtctggt ccggcgcgct cacctggatc
     3301 ctgctcaagc ttgtcgacgc gatcgcgggt atgcgcgttg ctgctgatga ggaaaccgag
     3361 ggactggaca ccgtgctgca caacgagaag ggctacaacc tgtaaggcat ggagcggcgt
     3421 ggcttcatca aattctgcgc agcatcggtc gccgccctca aagcgggcga ggtcgcggcc
     3481 gatgcgcgcg tgcagccgta cgcgcgcgca aagctcatgg acgagaaagg cgcgccgctg
     3541 cgcgccgggt caatcccggc ggaccgcaac ctcatcttcc actatccgta cgccgcgacc
     3601 ccctgttttc tcctgaacct cggtaagccg gtccggccgg cgcggctgaa gacgcactcc
     3661 ggcgaggcct acgagtggaa aggcggcgtc ggcgcgcagc gctcgatcgt cgcttactcg
     3721 gcgatctgcg cccaccgcct gtcgtatccc acgaaggaca tcagcttcat cagctaccgt
     3781 acggagaaaa gcgcgcgcaa ccgcatcgcc aacgtcatcc actgctgctc cgagcacagc
     3841 cagtacgacc cggcggaagg cgcgcgcgtc gtcgctggcc ccgcgccgca gcccctcgcg
     3901 gcgatcgtgc tcgaatggag ccaggccacc gacgaaatcg aggcggtcgg cacgctgggc
     3961 ggcgagatgt tccgcgagtt ctttgagaag ttcggcttca agctcgagat tgagcacggc
     4021 gcgccgcgcg cgacggtggc cggcacctgc gtggtgcagg agctcgacaa ttactgcaaa
     4081 caacaggtga agtgctagcc tggcttcagg gcaaggaccc cttcccgccg atcgagcggg
     4141 ccctcaaaaa cccgaacggc ctgctcgccg cgggcggcgc gctgaccgtc gagcgcctgc
     4201 tcgaggccta ccggcacggc gtcttcccct ggtactcggg tagcgagccg atcctgtggt
     4261 ggtcgcccga cccgcgcatg gtgctctact gcgacgagct gaaggtgtcc cggtcgctcg
     4321 cgaagaacat ccgcaacagg ggctatgagg tgcgcgtaga cagcgccttt gccgcggtgc
     4381 tcgacggttg cgccgaacgg ccgcagactt ggctcggacc ggacatgaaa aaggcctatg
     4441 tgcgcctgca ccgggccggc cacgcccatt ctttcgagac ctggcgcgac ggcgagatgg
     4501 tcggcggact ctacggcgtc gccatcgggc gcatgttcta cggcgagtcc atgttctcgc
     4561 gcgcgaccga cgcttccaaa gtggcgcttg tttcgctggt cgcggaactc cgcgcgcggc
     4621 gcttccctct catcgactgc cagatgcaca cgccgctcct cgcctcgctc ggcgcccggg
     4681 agatcccgcg gcgcgccttc ctgcgcgaat tgacggcgct ggtacactac ccgcagcagc
     4741 cgggaaagtg gaatgtcaag acttaacgac ctgccgcacg cagtactgca gttctatgtg
     4801 acggcgccct atccgtgcag ctacctgccc gaccgcatgg cgcgctcgca ggtggcgacg
     4861 ccaagccatc tcatcaacac cgagctctac ggcgatctgg tgaaagccgg cttccgtcgc
     4921 agcgggattt tcacctaccg gccgcattgc gacgcgtgcc gcgcctgcgt tccggtgcgc
     4981 attcccgtgg gccagttcaa caccaaccgg tcgcagcggc gcgcgtggaa gcagcactac
     5041 gcgctgaccg cgcacccgca gccgctcaag tttcgcctcg agcattacgc gctgtacctg
     5101 cgctaccagt ccaagcgcca ttccggcggc ggcatggaca acgacagccg cgaccagtat
     5161 tcgcacttcc tgctgcagag ccgcgtcgat acgcgcctca tcgaattccg cgacgcgggc
     5221 cagctcgtca tggtgtcgat cgtcgactac ctgcccgacg gcctgtcctc ggtttatacg
     5281 ttctacgagc ccgaccgccg caacgcgagc ttcggcacgt acaacgtact ctggcagatc
     5341 gagcaatgcc ggagccttgc gctgccctac ctgtacctcg gctattggat tcgcgagagc
     5401 ccgaaaatgg cctacaagtc caagttcgag ccgatcgagg gcttcgtcgg cggcgcctgg
     5461 cggcgcctcg gcgcgaaggt gtgagcctct acgggcgcgc ccgaccgctc cttttctcgc
     5521 tagatcccga aaccgctcat aggcttggcc tgcgcctcgc cgcgcttgtc ccccgcgcaa
     5581 cctttcgcgc cccggtcaag gctctgggcc tggagtttcc gaaccccgtg ggcctggcgg
     5641 cgggtctgga caaagacgcc gcgcacatcg atgcgctcgc gcgcctgggc ttcggcttca
     5701 tcgaggtggg caccgttacg ccgcaggcgc aacctggaaa cccgaagccg cggctctttc
     5761 gcctacccca ggcgcaagcg ctcatcaacc gcttcggctt caacaacgtc ggcgtcgacg
     5821 agtttctcgc caacgtgcgg cgcacgcgct ggcgcggcat tctgggcatc aacatcggaa
     5881 agaatgccac cacgcccgcc gagcgcgcgg tcgaggacta cgagcagtgc ctggcaaagg
     5941 tgtacgccgc cgcttcgtac atcacgatca acatctcctc tcccaacacc gctggcttgc
     6001 gcgcgctgca ggaaaaggac tcgctggagc atcttctcgc ccgcctcgtt tcgctccgcc
     6061 aaagaatgat cgtgcagcac ggcaaacacg tgccgctcgt cctgaaggtg gcacccgatc
     6121 tcgacgcgga tgacctgcgc gcgatcgccg aggcggtgaa gcgccacggc atcgaagcgg
     6181 tcattgccac gaatacgtcg acgtcgcgcg agggtgtgga acgcttgccg cacgctgccg
     6241 aagccggcgg gctatccggg gcccccatcc tgaagaaatc caccgccgtg ctgcgcgagc
     6301 tcgcacgttc cttgccgcaa acgacgctca tcggcgccgg cggaatcctg agcggcgcgg
     6361 atgcgcagca gaagttcgcc gccggcgcga gcctggtgca gctctacacc ggcctcatct
     6421 accgtggccc gtcgcttgtg ggcgaatgcg tatcggcata ccgcgcgaag taaaggacgg
     6481 cgagttccgc gtcgccctgt ccctcacggg ttcgtccaag gcgattccga ccccgaactc
     6541 aggacgggta ttcaggtgaa agcggtgcat actcgccgct atgaaaagag ccgcggtcgc
     6601 cctgatcgac gaaggccgct gcatcggctg cactttatgc atcgacgcct gcccagtcga
     6661 tgccgtcatt ggtgcgtcgc agctcatgca tacggtggtc gaaccctggt gcatcggctg
     6721 cgagctgtgc gtcgcgccct gcccgatcga ttgcatccgc atggtgccgg ccaagggtga
     6781 gtggaacgcc agcttgaagc gcgctgccgg cgaccgcggc cgcgctcgca cgaagaggct
     6841 ccaacgcgcg tccgtcccac ccggcccggt ggaccgcagc gcggtcctcg cagccgcttt
     6901 ggcgcgcaag aaaacctgat gtccgtggtt cgcgcctacc gcgaagacga ccttgacgcg
     6961 gtggcggcca ttcactcggt ctcgttcccg cggcagcttc gttcgcgcgt ctggatcgcg
     7021 gcaagtttcg gcgcctaccc gaagacgaga atgtatgtgg ctgagagcga tgcccgtgtc
     7081 atcggcttcg cgatgtggac cgaaaagagc ggctttcgcg cgcacgcggt gctcgagctc
     7141 gagcaaatcg ccgtggcgcc cgagcatcgg cgccgcggga tcgccgaagc gctcatccgc
     7201 cgctcgcttt cggaagtgga gaaggacctg gaaacgctga aagcggtcgt cgtcacgaca
     7261 cgcgcggaca acgccgcgca agcgctgtat cgcaagacgc tcggcgcgga ggtggaagcg
     7321 accatcgcgt cgctgtactc gggggacgaa gtgctgatgg tggctcgtct tcgatgaatc
     7381 cgcagaagcg gctcgaaatc tttcggcgcc tgcacgcggc gaatcccaag cccaccaccg
     7441 agctccgcta cacgacgccg tacgagctcc tagtcgcggt ggtactgtcg gcgcaggcga
     7501 ccgacaagtc ggtcaacctc gcgaccgcgg aactctttcg caaggcgaat actccggaaa
     7561 agatggtgaa gctcggcgtc gccggactcg aggagtacat caagcgcatc ggtctctacc
     7621 gcatgaaggc gaagaacgtg atcgcgcttt cgcagatcct gctcgaaaga cactgcggcg
     7681 cagtgccgca gaatcgcgaa gccctcgagg cactgcctgg cgtggggcgc aagaccgcga
     7741 acgtcgtcct gaacacggcg ttcgggcagc cgaccgtagc ggtcgataca cacatcttca
     7801 gggtctccaa ccgcaccgga atcgcgcccg gtaaggaccc cttcgaggtc gagctgaagc
     7861 tcctcaagtt cacgccgccc gagtatctga agaacgcgca ccactggctc atcttgcatg
     7921 gccgctacgt ttgcctggcg agaaagccgg ggtgcccgca atgcctaatt cgcgacctgt
     7981 gcgagttccg accgaagacc aaggatgttc cagccgaccc gagaccaggc aagagacacg
     8041 ttctttgaag cgtggcgcaa gtatcgggcc ggccagccgg tggtcggcat tgaggcgctc
     8101 gccctcgagg ttatcctgca gcacccggag taccacgccg ccctgagcga tccggagcgc
     8161 acgcgccagc gcgaatacgt tgacgaatcg aatccttttt tgcacatgag cctgcacgtc
     8221 gcgctcgaag agcagctctc catcgaccag ccgcccggta tcgttgcgcg cttccaggcg
     8281 ctggtccacc gctatggcga acgccacgac gcgctgcacc acgcgctcga atgccttgcg
     8341 gaaatggtct ggcgctcgca gcgcgatcag gcgccgcccg atgcgcgtgc ttatctcgac
     8401 tgtcttgcgg cgcgcgctct acgcaagtag ccggccgttg acaggcattg aattgagccg
     8461 acggaactaa cgcacgagcc aaggtggcgg catattccct gctgcatgat ggctcgtacg
     8521 aatccatcgg aaagagatcg cgccatgaaa gaacgctcca ccatcccggc acttttcatg
     8581 gcggcctgcg ccattcttgc gatcgcgtgg gcggcgcccg cgcggcccgc cggcctcgac
     8641 gactcgatca tcctcattgc gaagcgccag ctgcaggaca agctctacag ctcgacgatc
     8701 ctgctcgcgc gcccgatcgg cggcggccgg cacgtcggct tcatcatcaa caagcccacg
     8761 caggtcacgc tcggcaagct cttcccgacg cacgagccct cgcgcaaagt taccgaccct
     8821 gtgttcctgg gcggcccggt gagcccggaa gtaatcttcg cgctcgtcca gggcaagagc
     8881 agcccgggcg ggcgctcgat ccgtatcctg gacgaccttt acctcgcgat cgactccgac
     8941 gtcgtcgacc gcgtcatcga aaaacagtct tcgcacgcgc gcttcttcgc cggcatggtg
     9001 ctgtggcgtc ccggcgagct tgaggacgaa gtgaagaaag gattgtggta catgcaggac
     9061 gcccgcgccg atctcgtgct tcgcaaaccg accgactcga tgtacgagga gctcgtcggc
     9121 cgctccgagc gcaaagccaa cgcgatctag aaaaaggggt caggaccctt tttctgtaaa
     9181 aggggccgga ctcctcgtct ttcggtcgtt ccgatcgtca gccggaatcg ctgaccaaga
     9241 tccgacccct tttatggaaa aagggtcctg accccctttt atgggtctag tccttgcgct
     9301 gctcgtgcag gttggacttc tggctggaga accacgccgc caggtcctcg atgtcctgct
     9361 tggaaagctg cgcggcgaag cccttcatga tgggattgtt gcgccggccg ttcttatagt
     9421 cggtgatggc gcgcaccagg taatcgtagt actgccccgc gaggatcggc tgtgtcggat
     9481 cgga
//
LOCUS       DASTPD010000256         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_336990, whole genome shotgun sequence.
ACCESSION   DASTPD010000256 DASTPD010000000
VERSION     DASTPD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336990"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFR57_08485"
     CDS             <1..557
                     /locus_tag="VFR57_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU0259437.1"
                     /translation="IESAGAKTKKAQTIKSHHNVGGLPKSLKLKLLEPLRELFKDEVR
                     ELGLALGLPREMVFRHPFPGPGLGVRILGSINKDQVELLQRADDIFIQELRTSGWYDR
                     TAQAFAVFLPVRSVGVMGDGRTYENVVALRAVQTTDFMTADWAPLPHELLARVSNRII
                     NEVRGINRVTYDISSKPPATIEWE"
     gene            complement(568..1797)
                     /locus_tag="VFR57_08490"
     CDS             complement(568..1797)
                     /locus_tag="VFR57_08490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259438.1"
                     /translation="MASPFGVRSFRFQWPADLATSWAFEMEALILGWYVLSTTGSVQQ
                     LVIVGALAWLGSLFSPFFGLAGDRIGHRTLLCVTRGVYAVLAGVLAALTLNNALEPWH
                     VFAIAALAGLMRPSDMVMRHVLVGQTMQPGMLLAALGISRTTSDTARVAGALTGTAGV
                     TLVGMGPAYAVVTGMYIAAFALSLGVAGAPPAKRAAPAHPLADLKEGFSYVWRKPDLL
                     GAFTMAFLVNLLAYPVFLGLLPYVAKDVYAIGQSGLGYLAGAFWTGALAGSLSVGSSR
                     FRLRAARAMLWSGALWFAALMVFGQTTTLSVGVPLLFATGFVQSFCLTPLAAVMLRSS
                     DESMRGRVMGVRMLAIWGLPLGLLTAGPMIAAVGYPATTFVYAAVGLAATLATGYRWR
                     HALWSRSAAANAAYAGS"
     gene            1883..2287
                     /locus_tag="VFR57_08495"
     CDS             1883..2287
                     /locus_tag="VFR57_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259439.1"
                     /translation="MVVELPDPEEPDPVAPLEAPLLGEVLGEVVLLPEPVAPELEPDL
                     LKCASHSEREIWPSLLASIDEKVGAAALKLVLPDDIPDEPEDIPDEPLEPDAEGVVLL
                     PEVELCAIATLDRARSAAAVALVTSFNIDQSS"
     gene            2401..2811
                     /gene="tadA"
                     /locus_tag="VFR57_08500"
     CDS             2401..2811
                     /gene="tadA"
                     /locus_tag="VFR57_08500"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU0259440.1"
                     /translation="MDDDAFMRRALQLARRAEQEGEVPVGAVVVFGNDVAGEGWNRPI
                     AAHDPTAHAEIQAMRAAAHKVANYRLTGATLYVTLEPCDMCIGAMFHARIGRVVYGAT
                     DPKKLALKNQVALEGGVLAAECGALLSAFFAGKR"
     gene            2808..3269
                     /locus_tag="VFR57_08505"
     CDS             2808..3269
                     /locus_tag="VFR57_08505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0259441.1"
                     /translation="MTIEIDLARQELRYRDRIYPVSTSKFGPGEKNGSFRTPRGSHIV
                     RAKIGAGQPLGAVFVRRRPTGEVWTPELHSRYPGRDWILTRILWLSGCEPGKNRLGDV
                     DTMRRYIYIHGTPDSAELGKPGSIGCVRMRNRDVAELFDLVPPYTPVEISG"
     gene            complement(3259..>3615)
                     /gene="mtnA"
                     /locus_tag="VFR57_08510"
     CDS             complement(3259..>3615)
                     /gene="mtnA"
                     /locus_tag="VFR57_08510"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="isomerizes methylthioribose-1-phosphate into
                     methylthioribulose-1-phosphate; involved in methionine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEU0259442.1"
                     /translation="VDVVVVGADRIAANGDVANKIGTYALAVLAKRHGIPFYVAAPLS
                     TFDAKIPDGSHIPIEERPAAEVTGYGDTRWAPQGVAVRNPAFDVTPAELITGIICERG
                     IASPPNRQTIASLFNR"
BASE COUNT          689 a         1180 c         1230 g          516 t
ORIGIN      
        1 tgatcgagtc ggcgggtgcc aagaccaaga aggcgcagac catcaagtcg caccacaacg
       61 tcggtgggct gcccaagtcg ctcaagctca agctgctcga gccgctgcgc gagctgttca
      121 aggacgaggt gcgcgagctc ggcctcgccc tcggcctgcc gcgcgagatg gtgttccgcc
      181 acccgttccc ggggcccggg ctcggggttc gcatactcgg ctcgatcaac aaggatcagg
      241 ttgaactcct ccagcgcgcc gatgacatct tcatccagga gctgcgcacc tcgggctggt
      301 acgacagaac cgcccaggcg ttcgcggtgt tcctgccggt gcgctcggtc ggcgtgatgg
      361 gcgacgggcg cacctatgaa aacgtcgtgg cgctgcgagc cgtgcagacc acggacttca
      421 tgaccgccga ctgggcgccg cttccgcacg agctcctcgc gcgagtctcg aaccggatca
      481 tcaacgaggt gcgcggtatc aaccgagtca cctacgacat ctcctccaag ccgcccgcaa
      541 cgatcgagtg ggagtgagag atgcgagcta ggaccccgcg tacgcagcgt ttgcagccgc
      601 cgagcgcgac caaagcgcgt ggcgccatcg gtagccggtg gcaagcgtcg ccgcgagccc
      661 cactgcggca tagacgaagg tggtcgcagg gtagccgacc gcggcgatca tcggcccggc
      721 ggtgagcaac ccgagcggca acccccagat tgccagcatg cggaccccca tcactcggcc
      781 gcgcatggac tcatcggaag aacgcagcat gacggcggcg agcggggtaa ggcagaagct
      841 ctgcacgaag ccggtggcga agagcagggg aacgccgacg gagagtgtcg tcgtctgccc
      901 gaagaccatg agagcggcaa accaaagcgc gccgctccac agcatggccc gcgccgcacg
      961 cagccggaag cggctggatc ccacgctgag cgagcccgcc agcgctcccg tccagaatgc
     1021 cccggcgagg tagcccaagc ctgactgccc gattgcatag acatcctttg caacgtaggg
     1081 cagcaggccg agaaagacgg ggtacgcgag caagttaaca aggaaagcca tggtgaacgc
     1141 ccccagcagg tctggcttgc gccagacata gctgaagcct tccttgagat cggccagcgg
     1201 atgcgctggc gccgcgcgct ttgccggcgg agcgcccgcg acgccgagcg aaagcgcgaa
     1261 cgcggcgatg tacatgccgg taacgactgc gtacgccggg cccatcccga cgagcgtcac
     1321 gccggccgtg ccggtgaggg ctcccgcaac gcgcgcggtg tcggaggtgg tgcgcgagat
     1381 gccgagcgcg gcgagcagca tgcccggctg catagtctgc ccgacgagca catgccgcat
     1441 caccatgtcc gaaggacgca tcagcccggc gagggcggcg atcgcgaaca catgccaggg
     1501 ttcgagcgcg ttgttcagcg tcagtgctgc gagcaccccg gccagcacgg cgtacacgcc
     1561 gcgcgtcacg cagaggagcg tgcggtggcc gatgcggtct ccggcgagac cgaagaacgg
     1621 cgagaaaagc gacccgagcc acgccagcgc gccaacgatc acgagctgct gcaccgagcc
     1681 ggtggtggag agcacatacc agccgaggat cagcgcctcc atctcgaacg cccacgacgt
     1741 ggcgaggtca gccggccact ggaaacggaa gctgcgcacg ccgaagggcg aagccatcgg
     1801 cgaatgctac agggaacgca aaacgggccg cccgtccggc gcggcccgtt attgctgcga
     1861 ctacagccct gatgcgcgtt acttggtggt ggagcttcca gaccccgaag aacccgatcc
     1921 cgtcgccccg ctggaggcgc cgctgctcgg agaggtgctc ggagaagtcg tgctgctgcc
     1981 tgagcccgtc gcgccggaac tcgagcccga tttattgaaa tgcgcatccc actccgaacg
     2041 cgaaatctgg ccgtcgttgt tggcatccat cgacgagaag gtgggcgccg ccgcgctcaa
     2101 gctggtgctg cccgacgaca tccccgatga gccagaggac atcccggacg aaccgctgga
     2161 gcccgacgcg gaaggcgtcg tgctgctgcc cgaggtcgag ctttgtgcca tcgcgacgct
     2221 ggacagggcg agaagcgcag ccgctgtcgc actggtaacg agtttcaaca tcgatcaatc
     2281 ctcctaaagg aaaggtgagg ggtaatgcaa accggagcgt cgcaagcacc gtgccggagg
     2341 aaacatcggg catggcgaag agtccacgct cccgccctga taaaaattac aatcgcctgc
     2401 atggacgacg atgcattcat gcgccgggcc ttgcagctcg cgcgccgcgc cgagcaggag
     2461 ggcgaggtgc cggtcggcgc ggtcgtcgta ttcggaaacg acgtggcagg ggagggctgg
     2521 aaccggccga tcgcggcgca cgacccgacc gcgcatgccg agattcaggc gatgcgcgcc
     2581 gcggcacaca aggtggccaa ctaccgtctg accggggcga cgctttacgt gacgctggag
     2641 ccttgcgaca tgtgcatcgg tgcgatgttc catgcccgga tcgggcgtgt ggtgtacggg
     2701 gcgacggatc cgaagaagct ggcactgaag aaccaggtcg cgctggaagg cggcgtgctc
     2761 gctgccgaat gcggcgcgtt gctgagcgct ttcttcgccg gcaagcgatg acgatcgaaa
     2821 tcgacctcgc ccgccaggag ctgaggtacc gcgaccggat ctatcccgtt tccacttcga
     2881 agttcggacc gggagagaag aacggcagct tccgcactcc gcgcggaagc cacatcgtgc
     2941 gcgccaagat tggagccggg cagccgctcg gggcggtgtt cgtgcgccgg cggccaaccg
     3001 gcgaggtgtg gacgcccgag ctgcacagcc gctacccggg gcgcgactgg atcctgaccc
     3061 ggatcctctg gctatcgggt tgcgagcccg gcaagaaccg cctcggcgac gtggacacga
     3121 tgcggcgcta catctacatc catggcacgc cggacagcgc agagctcgga aagccgggct
     3181 cgatcggctg cgtgcgcatg cgcaatcgcg acgtcgccga gctgttcgac ctagtaccgc
     3241 cctatacgcc cgtggaaatt agcggttgaa aagcgaagcg atggtttggc ggttgggagg
     3301 tgaggcgatt cccctctcgc agatgatccc cgtgatgagc tccgcgggcg tgacgtcgaa
     3361 cgccgggttg cgcacggcga cgccttgcgg cgcccagcgc gtatccccgt atccggtcac
     3421 ctcggccgcc ggacgctcct cgatcgggat gtgcgagccg tccgggattt tggcatcgaa
     3481 ggtggaaagc ggcgcggcga cgtagaacgg aatgccgtga cgcttcgcca gcaccgccag
     3541 cgcgtaggtg ccgatcttgt tcgccacgtc gccgttggcc gcgatcctgt cggccccgac
     3601 caccacgacg tcgac
//
LOCUS       DASTPD010000257         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1358950, whole genome shotgun sequence.
ACCESSION   DASTPD010000257 DASTPD010000000
VERSION     DASTPD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358950"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFR57_08515"
     CDS             complement(<1..1155)
                     /locus_tag="VFR57_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259443.1"
                     /translation="MTTRVVSAKYVLGERDGAQVLLQEHYVYVDGSRIEAVTRDAPAP
                     GQEVIRYEHGFVMPGFVNLHAHCIRGGLFRGIADDLEMEPFVPKLVYNIILPLTALAA
                     KELTAQELRAVTALGMCDLLRGGTTTLLDMWHHGQEVFFDVARELGLRTVGAPFIMSA
                     SNSRLGPEGYPVWDFGNDGHAQLQKSIALFKARDEGHDGLAQVAIGPHATDTCLPELL
                     RDCRKTADELGCILTTHFAQTPQEVSLLRTKYGMGAAEYAQHAGLLGRNVVLAHAGCA
                     SDAELKILAETRTSVANCAFSFAREGISFPYARFARAGINTGIGTDSHGMDFISEARV
                     TGLLSKQHFGRAHFGTARELVRAGTVAGADALGRPDLGRLSPGARADLLVY"
     gene            complement(1152..2096)
                     /locus_tag="VFR57_08520"
     CDS             complement(1152..2096)
                     /locus_tag="VFR57_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259444.1"
                     /translation="MKKLVLAALLALPLLAEAQYPTKPIRAVVAFGTGGTTDVTARII
                     AAALAQSLGQQVVIDNKPGADGIIAGSDVVKSAPDGYTIWFATYTQISALPSLRKNVP
                     FDPIADLTPIGGIGNFSFFWAVNPELPIKNLKELAAYGRANPGKLNAGSSGAAATMAP
                     TAFARAEKFDLQVVAYKSETTALNDLVTGRIQLMLASGTLAQHLRDGRARGIATLGNQ
                     RSKALPELPTMAESGVAPFPAVPWMGLFGPGKMPREIVERLSRELLAVLKNPDVRERV
                     ERQAIDINPLTQEAISALAKEQTEVWRRITKEAGYIPE"
     gene            complement(2153..2494)
                     /locus_tag="VFR57_08525"
     CDS             complement(2153..2494)
                     /locus_tag="VFR57_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259445.1"
                     /translation="MSAVVWALSMPLTGKMEPWDAESPYYFVALAVAGAVSGAIVPRH
                     LWAHYLGAVLGQTAYELAFLKLGPLFPLGLVFLAGYSLIFLASAALVASFRKNPPGEG
                     KPPQTPLHGRP"
     gene            complement(2540..3244)
                     /locus_tag="VFR57_08530"
     CDS             complement(2540..3244)
                     /locus_tag="VFR57_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0259446.1"
                     /translation="MMLAEALKAKIKVCMFDQYGTVVDMQKGLTEVVTPYLKEKRWNG
                     RPDAFVTWWRRTHFENSMIDALLHREHTPYREIGHRAVAYTLERAGIPYTMDEVRYLV
                     AQIERLKPFPDVPEALARLKTRYKIVVLSNGDPDMLETAKAFHKIPFDAVISVAVANS
                     FKPHVATYTKAAELMGVRMDEVLFVANHAFDCIGAKSAGMHAAFIDRRKRPFGDTPHQ
                     PDIVVQDMKTLAEMIG"
     gene            complement(3354..4067)
                     /locus_tag="VFR57_08535"
     CDS             complement(3354..4067)
                     /locus_tag="VFR57_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0259447.1"
                     /translation="MLRRPEFGRIRSALAASSHFQSLDGSDLDRLAQLGRLHHLRHGE
                     RAARNGAYEDRFYLILSGAVRVSSQMPGAKEFVFAVLGPGSYFGLATAVRHAAFTADA
                     RAFGPTDLAVFPGSSLVELLDERPRLWKHVCGLLSRRLRLALLVLRDNSVAPLPERIA
                     RRLLGHALSSDLRGSREIDLRMTQADLALMLGASRARTNAALKALERTGLVRVGYRGI
                     RILDLAALRRLAGPGIHAY"
     gene            4176..4802
                     /locus_tag="VFR57_08540"
     CDS             4176..4802
                     /locus_tag="VFR57_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0259448.1"
                     /translation="MRQGPAKSDLTVMLGRALDLLDRALVIVTAAGELVFRNRQAARL
                     LAAGHGGLRITGGALSSNSTRVRVALAEAISLACNALQPTGVCLPQPCSPPERWLRLA
                     VSPIHAGGSARRAGCAAVWIVRSGYPSRPNEVLLAALFGLTPAEARLAGAMLTGCAAN
                     EYARQAGVGVVTVRSQLHSIFVKTGVRRQAQLVALLSKVPALTLTDQA"
     gene            4799..4993
                     /locus_tag="VFR57_08545"
     CDS             4799..4993
                     /locus_tag="VFR57_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259449.1"
                     /translation="MKPDPPRGRHGIEMRTLPCHVAREFDEKADDGDFARGKMRASAS
                     SWVIDGESDPIPVVAIALPD"
     gene            4998..5195
                     /locus_tag="VFR57_08550"
     CDS             4998..5195
                     /locus_tag="VFR57_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259450.1"
                     /translation="MDSTDLFHAVLFRAAELLGGTEPLARRLHVSAEELTRWLAGEGI
                     VDIGILPRAIGVLLDPSPPPN"
     gene            5709..5915
                     /locus_tag="VFR57_08555"
     CDS             5709..5915
                     /locus_tag="VFR57_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0259451.1"
                     /translation="MIGDARLTAREIDVLRLLSLGYTHASAASRLGLSAHTVTSHVKK
                     IYAKLGVHTAGAAVMRAMKLGIIE"
BASE COUNT         1044 a         2022 c         2098 g         1119 t
ORIGIN      
        1 gtacaccagc aggtccgccc gggcgcccgg cgacaggcgg ccgaggtcgg ggcggccgag
       61 cgcatcggcg cccgccaccg tccctgcgcg cacgagctcg cgcgcggtac cgaagtgcgc
      121 tcttccgaag tgctgcttgg agagcaggcc cgtgacacgg gcctcgctga tgaagtccat
      181 gccgtgcgag tcggtcccga tgccggtgtt gatgccggcg cgcgcgaagc gtgcgtacgg
      241 gaaactgatg ccttcgcgcg cgaagctgaa cgcgcaattg gcaacgctgg tccgcgtttc
      301 ggccaggatc ttcagctccg cgtcgctcgc gcagccggcg tgcgccagga ccacattgcg
      361 gccgaggagc ccggcgtgct gcgcgtactc cgccgcgccc attccatact ttgttcttag
      421 aagagaaacc tcctggggcg tctgcgcgaa gtgcgtggtc aggatgcagc ccagctcgtc
      481 cgccgttttg cggcagtcgc gcaggagctc gggcaggcag gtgtcggtcg cgtgcggccc
      541 gatcgccacc tgagcgaggc catcgtgccc ctcgtcgcgc gccttgaaga gcgcgatgga
      601 cttctgaagc tgcgcatgac cgtcgttgcc gaagtcccac accggatagc cctccgggcc
      661 gaggcgcgag ttcgacgccg acatgatgaa cggcgcgccg acggtgcgca ggccgagctc
      721 gcgcgccacg tcgaagaaca cctcctgccc gtggtgccac atgtcgagga gcgtcgtcgt
      781 cccgcctctt aggagatcgc acatcccgag cgcggtgacc gcgcgcagct cctgcgcggt
      841 cagctccttc gccgcgagcg cggtgagcgg cagaatgatg ttgtagacga gcttcgggac
      901 gaagggctcc atctcgaggt cgtcggcgat gccgcggaag agccccccgc ggatgcagtg
      961 cgcgtgcagg ttgacgaagc ccggcatgac gaagccgtgc tcgtagcgga tgacttcctg
     1021 ccctggcgcg ggcgcgtcgc gcgtgaccgc ctcgatgcgc gaaccgtcga catagacgta
     1081 gtgctcttgc agcaggacct gcgcgccgtc ccgctcgccg aggacgtact tcgcgctgac
     1141 gacccgcgtg gtcactcggg gatgtagccg gcctctttcg tgatgcgccg ccagacttcg
     1201 gtctgctcct tcgccagggc gctgatcgct tcctgagtga gcggattgat gtcaatggcc
     1261 tgacgctcga cgcgctcgcg cacatccgga ttcttgagca ccgcgagcaa ctcacgcgaa
     1321 agccgctcga cgatctctcg cggcatcttc cccggtccga agaggcccat ccacggcacg
     1381 gccgggaagg gtgcgacgcc cgattccgcc atggtcggca gctccggcag cgctttgctg
     1441 cgctggttgc ctagcgtagc gattccgcgc gcgcggccgt cgcgcagatg ttgcgccagc
     1501 gttccgctcg cgagcatgag ctggatacgg ccggtgacga ggtcgttaag cgccgtggtc
     1561 tcgctcttgt acgcaacaac ctgcaggtcg aacttctcgg cgcgggcgaa cgccgtcggc
     1621 gccatcgttg ccgcggcgcc cgagctcccc gcgttcagct tcccgggatt ggcgcgacca
     1681 taggctgcga gctccttgag gttcttgatc ggcagctccg gattcaccgc ccagaagaag
     1741 ctgaaattac cgatgccgcc tatgggcgtg aggtcggcta tggggtcaaa cggaacattc
     1801 ttgcgcagcg aaggcagcgc ggagatttgc gtgtacgtcg cgaaccagat cgtgtagccg
     1861 tccggcgcgc tcttcacgac atccgatccc gcgatgattc catccgcgcc cggcttgttg
     1921 tcgatcacca cctgctggcc gagcgactga gcgagcgcgg ccgcgatgat gcgcgccgtc
     1981 acgtccgtgg tgccgccggt tccgaaggcg accaccgctc ggatgggttt cgtcggatat
     2041 tgcgcctcgg cgagaagcgg aagcgcgagg agggcggcaa ggacaagttt tttcatgagg
     2101 ggtttcttgg cgtcactacg aagcggcgag ccgatgataa agcgaaagcg cgctatggac
     2161 ggccgtggag cggcgtttgc ggcggctttc cttcaccggg cgggtttttt ctgaacgacg
     2221 ccacaagcgc cgcgcttgcc agaaagatca ggctgtagcc ggccaagaac accaatccaa
     2281 ggggaaagag tggaccgagc ttgagaaaag caagctcgta ggcggtctgc ccgagcaccg
     2341 ctcctaggta gtgggcccat agatgccgag ggacaatggc gcccgagacg gcgcccgcca
     2401 cggcaagtgc gacaaagtag tacggcgact ccgcatccca cggttccatt ttcccggtca
     2461 gcggcatgga gagcgcccag acaacggcgc tcaatgcagc ggcaacaagt acggctagaa
     2521 ccgcgcggct attcacgctt catccgatca tttcggcgag ggtcttcatg tcctgcacga
     2581 caatgtccgg ctggtgcggg gtgtcgccga aggggcgctt gcgccggtcg atgaacgccg
     2641 catgcatgcc tgctgacttt gctcctatgc agtcgaacgc gtggttggcg acgaagagaa
     2701 cctcgtccat tctcactccc atgagctcgg ccgccttggt gtaggtcgcc acgtggggct
     2761 tgaacgaatt agcgactgcc accgaaatca cggcgtcgaa cggaatctta tggaaggcct
     2821 tcgcggtctc cagcatgtcg gggtcgccat tcgagagcac cactatcttg taacgggtct
     2881 tcaagcgtgc tagcgcctca ggcacgtctg ggaacggctt gagccgctcg atctgggcga
     2941 ccaggtagcg cacctcatcc atcgtgtagg gaatgccggc gcgctccagg gtgtacgcga
     3001 ccgctcgatg tccgatctca cggtatggcg tgtgctcgcg gtgcaggagc gcatcgatca
     3061 tggagttctc gaagtgggta cgccgccacc acgttacgaa agcatccggc cggccgttcc
     3121 agcgtttttc cttgaggtag ggagtaacca cctcggtcaa gcctttctgc atgtccacga
     3181 ccgtgccgta ctggtcgaac atgcagacct tgatcttcgc cttgagcgcc tctgccagca
     3241 tcattaaacg cctcgtcttc gtttgggtgg cctgacgtcg cgttttgccg caccgtcaac
     3301 cttcttcacg ctatgtgctg agggcgcgcg gtccgcgctg cggtcatgaa aatctaatag
     3361 gcgtgtatcc ccgggccggc tagcctgcgc agcgcggcga ggtcgaggat tcggatgccg
     3421 cgatatccga cccgcacgag gccggtgcgc tcgagcgctt tcagcgcggc attcgtgcgc
     3481 gcgcggcttg cgccgagcat gagcgcgagg tcggcctggg tcatgcgcag gtcgatctcc
     3541 ctcgagcctc gcaggtcgct gctcagggca tggccgagca ggcgccgcgc gatgcgctcg
     3601 ggaagcggcg cgacgctgtt gtcgcgcaat accagaagcg cgaggcgcag gcggcgcgaa
     3661 aggagcccgc agacgtgctt ccacagccgc gggcgctcgt cgagcagctc gaccagggag
     3721 gatccgggaa agaccgcgag atccgtggga ccgaacgcgc gcgcatcggc cgtgaaggcc
     3781 gcgtgccgaa cagcggtcgc cagaccgaag tagctacccg gcccgagcac cgcgaagacg
     3841 aattctttgg cgccgggcat ctggctgctc acgcgcaccg cgccgctcag gatcaggtag
     3901 aagcggtcct cgtacgcgcc gttgcgtgcc gcgcgttcgc cgtgccgcag gtggtgcagg
     3961 cggcccagct gtgccagccg gtcgaggtcc gagccgtcga gcgactggaa gtgcgagctc
     4021 gcggcgagcg cgctgcggat acggccgaac tccgggcgcc tcaacattgg tcgatcgtct
     4081 tcccggggac attctgcggc attttccgcg cggattgttc ccaccggcgc aatcgtccag
     4141 gccggcggca cagcgccggc tccaccattt gatggatgcg ccaaggcccc gccaaaagcg
     4201 atctcacggt gatgctggga agagcgctcg acttgctcga ccgcgcgctc gtcatcgtca
     4261 ccgcggctgg cgagctcgtg ttccgcaatc gccaagccgc gaggttgctc gcggccggcc
     4321 acggcgggct gcgcatcacc ggcggcgcgc tttcgtcaaa ttccaccagg gtgcgcgtag
     4381 cgctcgctga ggcgatcagc ctagcgtgca acgcgctgca gccgacgggc gtttgcctgc
     4441 cgcagccctg ttctccgccc gagcgttggc tccgcttggc cgtctcgccg atccatgccg
     4501 ggggctcggc gcggcgtgcg gggtgcgccg ccgtctggat cgtgcgatcc ggctatccca
     4561 gccggccgaa cgaagtattg ctcgcggcgc tatttggcct gactccggca gaggcccgcc
     4621 tcgcgggcgc gatgctgact ggttgcgccg cgaacgagta cgcccgccaa gccggcgtgg
     4681 gtgtcgtgac ggtccgctcg cagcttcaca gcatcttcgt caagactggt gtgcggcgcc
     4741 aggcgcagct cgtcgcgctg ctctcaaagg tgccggcgct gacgctcacg gaccaagcat
     4801 gaagcccgat cctccgcgcg gtcgtcacgg gatcgagatg cggactctgc cgtgccatgt
     4861 ggcgcgcgag ttcgacgaga aggcggacga cggcgacttc gcaagaggaa agatgcgcgc
     4921 aagcgcgtcg tcgtgggtga tcgatggtga aagtgacccc ataccggtgg tggcgatcgc
     4981 gctacctgac tgaatccatg gactcgacag acctcttcca tgcggtgctg tttcgcgccg
     5041 cagagctcct cggcggcact gagccgctcg cgcgtcggct gcacgtctcg gcggaggagc
     5101 tcacgcgctg gcttgccggg gagggcatcg tggacatcgg catcctgcct cgcgccatcg
     5161 gcgtgcttct cgacccgagt ccgccgccga actgaatctt gccttcctac gtaccgtact
     5221 ggccgtcgag gtggcccgcg gcaacagcgc cgtcgtatgt ctacgcgtta tgacggctgc
     5281 gccggcatta cagcttcgac ggcgtgccaa tcgagccgaa gtcaggccaa tctttgccgt
     5341 tcgtcctaag accctagacc ggtcaccatt tgccggagac gcatgtggga cgggggcgcg
     5401 tgctttgccg cgcccgagca aacgttcccg gtattaacgc cccatccaac aaccagtgga
     5461 tgcgctcacc aggcttcttg tggcagcgtg agatacaggg aaggtgggcc gtagcgaccc
     5521 accaataggg gtggccgatg agccagaaaa cgtcgatcaa tgtcgagccg cttacgtgcg
     5581 cgatcggtcc ggagtcgcgt aacgaaccta gggggcaact gggggatctc cgagcgctgg
     5641 attcgcggtg cctgggttgc tggcgactcc taagaccagg tgatgctgcg gcgtcgccag
     5701 cgcgtgtaat gatcggcgac gctcgcctga cggctcgaga aatcgacgtc ctgcgcctct
     5761 tgtcgctcgg ctacacgcac gcaagcgccg cgagcaggct cggcctctcc gcgcataccg
     5821 tcacaagtca tgtaaagaag atctacgcca agctgggcgt gcacaccgcc ggcgccgctg
     5881 tgatgcgcgc gatgaagctc ggaatcatcg agtaagggga cgccaagcgg aggagccccg
     5941 ttcgggcggg ggcttttcat ttcggccgct ttctggctcg gccagaagca ttgaagggtg
     6001 gtgtaagaaa tttctgtccg ggcggggcaa cgatcttctg ccgtcttacc cagtaacggc
     6061 cctaccgctc tgtccgcttt gccatgtcga tcggactact gcctacgtac gtccgcagcg
     6121 cttcgacgtt aggtttgccg tgatcgcaga acgccgctat tcgttctaga aggtggagcc
     6181 ggtcgcgcgt aggaaccatc gtaaaggggc ggccaggcag gaaacggccg cctttacagt
     6241 aggccaggct cagataagtg ccagtcggcc ctcgccgcgt ggt
//
LOCUS       DASTPD010000258         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_339378, whole genome shotgun sequence.
ACCESSION   DASTPD010000258 DASTPD010000000
VERSION     DASTPD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339378"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFR57_08560"
     CDS             <1..1293
                     /locus_tag="VFR57_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0259452.1"
                     /translation="AVPQAFIFNTRKTVYQEQIGATLSHRVSSSSTVQATVYGGSRWV
                     EQYLGFAGGAPPPTTSGGVVQLDRGYGGGALRFSTGEVLRVSLGAEYDRMEERRKGFV
                     NNNGVQGELRRNEDDSVWSTDFYAQAEWRFAERWSAHGGVRASRVEFESTDFFIVPGN
                     PDDSGSRAYSATTPVAGLLYRYSKTVSFYGNLGRGFETPTFAELAHQNPPASGLNFAL
                     EPARSRHAEAGVKAIIPNRARLNAALFGIETEDEIVVDVNMSGRTTFKNAARTERRGF
                     ELAAETLMPGPFGAQFAYTHLDATFKEGFTSTVMGNTVTVPAGNMLPGVPKSQAYARL
                     SYRQPRFYTYLEALYRSRVPVDDVNSEFADAYSVFNLVGGLVQQGPGWRISEYVRLDN
                     FTDRNYAGSVIVNEGNRRFYEPAPPRNISAGIQASLQF"
     gene            1324..2553
                     /locus_tag="VFR57_08565"
     CDS             1324..2553
                     /locus_tag="VFR57_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0259453.1"
                     /translation="MKTIIAVAVAAAFAAQALAQDKPKPPPAWHQGKPPAMAESKLAP
                     HAGKMTETPPSDILVSKLKVPAGFKVDLWASGLPGGRAMAVSDDGKKVYVGTRIIGRV
                     YEVSDDGGKRTVRVVVDKLTQPAGVAFKDGALYVFAIDKVLKYDGIASKPNVEPQDLT
                     AKFDLPPEQHHNWKYVAFGPDGKLYVPFGSPCNICEPPSKEYGQIRRYNADGSGKEVI
                     ATGVRNSVGFDWHPKTKELWFTDHGRDWAGDKGFEDELNKVSKPGAFYGFPYCHAMGQ
                     PDPDVKKPDPCKGVTRPVALLGPHAAGMGIKFYTGKMFPAEYQNTAFIARKGSWNREK
                     PFGFDVVNVREGEGGKVSIRPFMTGFQEGGEGYKFWGRPAYIAQMPDGALLVSDEQVG
                     AIYRVSYQKPKAPAKKQ"
     gene            2554..3138
                     /locus_tag="VFR57_08570"
     CDS             2554..3138
                     /locus_tag="VFR57_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0259454.1"
                     /translation="MFFKWKGRWALLTVLFSFSCFSQERLQLCAACHGEDGNSANPQI
                     PSIAGQPKLFLENQLILFREELRRSDQMLPVVKGLKDPEVVKLAEHFSKLPAKGMESG
                     AADPKLMKIGVEKARALRCGVCHLSDFSGQNQIPRLAGQREAYLEAEMRAYRDGKRTG
                     GDTIMAATLYGVADADIKALAHFLSRSNARSPGR"
     gene            3074..>3470
                     /locus_tag="VFR57_08575"
     CDS             3074..>3470
                     /locus_tag="VFR57_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259455.1"
                     /translation="MPTSRRSLTFFREATLDLPGVEPGGVLGKLVGDEHHGAVRFAES
                     GDGGTRVSVELSYIPPAGAFGHAVAAIFGADPKSEMDADLLRMKSVIETGHPPMRQSL
                     ARCRARARRLLLWRGPLSRGSRAARKAGCR"
BASE COUNT          658 a         1129 c         1155 g          528 t
ORIGIN      
        1 gcggtgccgc aggcgttcat cttcaatacc cggaagaccg tctaccagga gcagatcggc
       61 gcgacgcttt cgcatcgggt gagctcgagc tcgaccgtcc aggcgaccgt gtacggcgga
      121 agccgctggg tcgagcagta cctcggcttt gccgggggcg cgccgccgcc caccacttcc
      181 ggcggcgtgg tccagctcga ccgcggctac ggcggcggcg cgctgcgctt ctccacgggc
      241 gaggtcctgc gcgtctcgct cggagcggaa tacgatcgca tggaagagcg ccgcaagggc
      301 ttcgtgaaca acaacggcgt gcagggcgag ctgcggcgca acgaggacga ctcggtgtgg
      361 agcaccgatt tctacgcgca ggcggagtgg agattcgccg agcgctggag cgcgcacggc
      421 ggagtgcgcg cgagccgcgt ggagttcgag tcgaccgatt tcttcatcgt gcccggcaac
      481 ccggacgaca gcggcagccg cgcgtactcg gcgacgacgc cggtcgccgg cctgctttat
      541 cggtactcga agaccgtgag cttctacggg aacctcgggc gcggcttcga gacgccgacc
      601 ttcgccgagc tcgcgcacca gaacccgccg gcgagcggcc tcaacttcgc gctcgagcct
      661 gcgcgcagcc ggcacgccga ggcgggcgtc aaggcgatca tcccgaaccg ggcaaggctc
      721 aacgcggcgc tattcggcat cgagaccgag gacgagatcg tggtcgacgt caacatgagc
      781 ggccgcacga cgttcaagaa cgcggcgcgc accgagcggc gcggattcga gctcgccgcg
      841 gaaaccctga tgccaggtcc gttcggcgcg cagtttgcct acacccacct ggatgcgaca
      901 ttcaaggagg gtttcaccag cacggtgatg ggcaacacgg tcactgtgcc ggcgggaaac
      961 atgcttccgg gcgtgccgaa gagccaggcg tatgcccggc tcagctaccg gcagccgagg
     1021 ttctatacct acctggaagc gctataccgc tcccgagtgc ccgtcgacga tgtaaattcg
     1081 gagttcgccg acgcctacag cgttttcaac ctggtcgggg gcctggtgca gcagggcccg
     1141 ggctggcgga tcagcgagta tgtacgactg gataacttca ccgaccggaa ctacgccggc
     1201 tcggtgatcg tcaatgaggg caaccggcgc ttctacgagc ctgcgccgcc gcgtaacatc
     1261 agcgctggca tccaggccag ccttcagttt taatagcatc cccagaggag gcacgaaacc
     1321 accatgaaga caatcatcgc agtagcggta gccgccgcat tcgcggcgca ggcactcgca
     1381 caggacaagc ccaaaccccc tcccgcgtgg caccagggca agccgcccgc gatggcggag
     1441 tcgaagctcg cgccgcatgc cggcaagatg accgagaccc caccgagcga catcctggtc
     1501 agcaagctga aggttccggc ggggttcaag gtcgacctat gggcgagcgg gcttcccggc
     1561 ggacgcgcca tggcggtcag cgacgacggc aagaaggtgt acgtaggcac gcgcatcatc
     1621 ggtcgcgtgt acgaagtaag cgacgacggc ggcaagcgca ccgtgcgcgt cgtcgtggac
     1681 aagctcaccc agcccgcggg cgtcgccttc aaggacggcg cgctctacgt gttcgcgatc
     1741 gacaaggtgc tgaagtacga cggcatcgcg tcgaagccga acgtggagcc ccaggacctc
     1801 acggcgaagt tcgacttgcc gcccgagcag caccacaact ggaagtacgt agccttcggc
     1861 ccggacggca agctctacgt gccgttcggc tcgccgtgca acatctgcga gccgcccagc
     1921 aaggagtacg gtcagatccg ccgctacaac gccgacggct ccggcaagga ggtgatcgcc
     1981 accggcgtgc gtaactcggt gggcttcgac tggcacccga agaccaagga gctgtggttc
     2041 accgaccacg gtcgcgactg ggcgggcgac aagggcttcg aggacgagct gaacaaggtg
     2101 agcaagcccg gcgccttcta cggcttcccg tattgccacg cgatggggca gccggatccg
     2161 gacgtcaaga agcccgatcc ctgcaaggga gtcacccggc ccgtggccct gctcgggccg
     2221 catgccgccg gcatggggat caagttctat acgggcaaga tgttccctgc cgagtaccag
     2281 aacaccgcct tcatcgcgcg caagggctcg tggaaccgcg agaaaccctt cggctttgac
     2341 gtggtgaacg tgcgcgaagg cgagggtggc aaggtctcca tccgaccgtt catgaccggc
     2401 ttccaggaag ggggcgaggg ctacaagttc tggggccgcc cggcgtacat cgcgcagatg
     2461 cccgacggcg cgctgctcgt gtccgacgag caggtcggcg ccatctaccg cgtgagctat
     2521 cagaagccaa aggcgcccgc gaagaagcaa tagatgtttt tcaagtggaa agggcggtgg
     2581 gccctgctca ccgtcctttt ttctttttcc tgcttttcac aggaaagact ccagctgtgc
     2641 gccgcatgcc acggcgagga cggcaactcc gccaatccgc agataccctc gatcgccggg
     2701 cagccgaagc tcttcctcga gaaccagctc atcctctttc gcgaggaatt gcgcaggtcg
     2761 gaccagatgc tgccggtcgt aaagggcctg aaggaccccg aggtcgtcaa gctggccgaa
     2821 cacttctcga agttaccggc gaaggggatg gaaagcggag cggcggatcc gaaattgatg
     2881 aagatcggag ttgaaaaagc aagagcgctt cgctgtgggg tgtgccacct gtccgacttc
     2941 agcggccaga accagatccc gcgcctcgct ggccagcgcg aggcttacct cgaggccgag
     3001 atgcgcgcct accgcgacgg caagcgcacg ggcggcgaca cgatcatggc ggccacgctg
     3061 tatggggtag ccgatgccga catcaaggcg ctcgctcact ttctttcgcg aagcaacgct
     3121 cgatctcccg ggcgttgagc caggaggggt ccttgggaag ctcgtcggcg acgagcacca
     3181 cggcgcggtg cgcttcgcgg agagcggcga cggcggcacg cgcgtcagcg tggagctctc
     3241 ctacatcccg cccgcaggcg ccttcggcca cgcggtcgcc gccatctttg gcgccgaccc
     3301 caagagcgag atggacgccg acctgctgcg catgaaatcc gtgatcgaaa ccggccaccc
     3361 gccgatgcgg caaagcctgg cgcgctgccg cgcgagagct aggcggctct tgctttggcg
     3421 gggcccgctt tctcgaggat ctcgcgcagc acgtaaggca ggatgccgcc
//
LOCUS       DASTPD010000259         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_133560, whole genome shotgun sequence.
ACCESSION   DASTPD010000259 DASTPD010000000
VERSION     DASTPD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133560"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1083
                     /locus_tag="VFR57_08580"
     CDS             277..1083
                     /locus_tag="VFR57_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0259456.1"
                     /translation="MILLSWNVQWCRGMDERVDPQRIAREAKRLADPDVFCLQEIARN
                     FPEMAGSAGEDQVEELKKAMPGYEAFYAPGVDIPGKKGRRQFGNLILTRLPVGRVMRH
                     QLPWPAAPDVQSMPRAAVEVVVQAPFGPLRVTTTHLEYYSPRHRAAQIDRLCELHAEA
                     CVEHAGVSEPGSYESYERAKSAIVCGDFNLKPDDPHHRRMLDGGFVDAWLAMNEGKAR
                     QPTFHLFDGTTPYCCDYVFLTPDLVPRLKSIRIDSETQASDHQPVIVELA"
     gene            1076..1891
                     /locus_tag="VFR57_08585"
     CDS             1076..1891
                     /locus_tag="VFR57_08585"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0259457.1"
                     /translation="MLKVTKLAEALGAEIGGVDLSRPIGDFFPELRAAWLEHLVIRIR
                     GQKLTDPQVLAFSRNFGELDPPGPNPLGRPFLADHPEMNVISNIKENGVPIGGLGDGE
                     AIWHADMTYVERPPMAAILYAIEIPPAGGDTYWANMYLAYETLPAELKQRIEGREAVH
                     DATYNSAGVMRKGFKPGARHPLVRTHPETGRRALFLGRRRNSTIAGMELEESERLLDE
                     LWRHATQPQFTFRQQWRVGDVVVWDNRCTLHRRDAFDPRARRLLHRTQIRATA"
     gene            complement(1875..2264)
                     /locus_tag="VFR57_08590"
     CDS             complement(1875..2264)
                     /locus_tag="VFR57_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEU0259458.1"
                     /translation="MFDWRRPKNLGVMGGRLAPCRSTPNCVSSQADPADQEHYTAPIA
                     FQGGDAIAAVRKAVESMPRARVISADSHYLYAEFRSALLGYVDDVEFHYDGSVIHVRS
                     ASRLGRRDFGVNRARVEELRKRIKPSP"
     gene            complement(2277..>2813)
                     /locus_tag="VFR57_08595"
     CDS             complement(2277..>2813)
                     /locus_tag="VFR57_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEU0259459.1"
                     /translation="ELVPTARQLGVFWGYAPPAFPAVETDLALGEMQTAATAMNIKIR
                     PWLNRDEASLEANLAAAESSGVDALFVSAGGPQSQPKTISRIADFVLKRKLPTACDAA
                     GVYFMQGGVIAYSAEFSELGRRAAAYVDRILRGAKPGEMPIERPARFELVVNRRFAKA
                     LGFAIPPSLAVRADRFVD"
BASE COUNT          518 a          924 c          894 g          477 t
ORIGIN      
        1 catgccgtgt acgggcaccg agtcggcgag ctcttcgagc aggttcttca ataccggcca
       61 ggcagccggc tgtgctgcaa ccaatatccg atgcctgctt cccataccgc gccttccatt
      121 attaagccca atgtacgcgc cttttgcccg gttgttgagg ggattagccc cttccatggt
      181 caaactaaag tcatgcaaac ggacctatcc tatccattga agtaaagaga tcgcatatcg
      241 cctaagtagt taacttatgc cccgcttcct ctctttatga tcttgctcag ctggaacgtg
      301 cagtggtgcc gcggcatgga cgagcgtgtc gatccgcagc gcatcgcgcg cgaagcgaag
      361 cgcctcgccg acccggacgt tttctgcctg caggaaatcg cgcgcaattt tccggaaatg
      421 gcgggtagcg cgggcgaaga tcaggtggaa gagctcaaga aggcgatgcc cggctacgag
      481 gcgttctacg cgccgggcgt ggacattccc ggaaagaaag ggcggcgcca gtttggcaat
      541 ctcatcctga cgcgattgcc agtggggcgc gtgatgcggc accagctgcc ttggccggcg
      601 gcgcccgacg tgcagagcat gccgcgcgcc gccgtcgaag tggtggtgca ggcgccgttc
      661 gggccgctgc gcgtcacgac aacgcatctc gagtactact cgcctcggca ccgcgctgcg
      721 cagatcgacc ggctctgcga gctgcacgcg gaagcgtgtg tcgagcacgc aggagtgagc
      781 gaacccggaa gctacgaatc gtacgagagg gcgaagagcg cgatcgtctg cggcgacttc
      841 aatctgaagc cagacgatcc gcatcatcgc aggatgctcg acggcggctt cgttgacgcg
      901 tggctggcga tgaatgaagg caaagcgcgc cagccgacgt ttcatctttt cgacggcacg
      961 acgccgtatt gctgcgacta cgtatttctc actccggatc tcgtgccgcg gctaaaatcg
     1021 atccgcatcg attccgagac gcaggcctcc gaccaccaac cggtcatcgt ggaacttgct
     1081 taaggtcacg aagcttgccg aagcgctggg cgccgagatc ggcggcgtcg atctctcgcg
     1141 cccgatcggc gacttctttc cagagcttcg cgccgcctgg ctcgagcacc tggtgatccg
     1201 catccgcggc cagaagctca cagacccgca ggtgctcgcc ttcagccgca acttcggcga
     1261 gctcgatccg cccggaccga atccgctcgg ccggccgttc ctcgccgacc atccggagat
     1321 gaacgtcatc tccaacatca aggaaaacgg cgtgccgatc ggcggactgg gcgacggcga
     1381 ggcgatctgg cacgcggaca tgacctacgt cgagcgtccg ccgatggccg cgatcctcta
     1441 cgccatcgag attccgcccg caggcggcga cacgtactgg gccaacatgt acctggccta
     1501 cgagacgctg cccgcggagc tcaagcaaag gatcgaaggc cgcgaagcgg tgcacgacgc
     1561 cacctacaac agcgcgggcg tgatgcgcaa aggattcaag ccgggtgcgc gccatccgct
     1621 cgtgcgcact catccggaga ccggacgccg cgcgctcttt ctcggccggc ggcgcaacag
     1681 caccattgcc ggcatggagc ttgaagaaag cgagcggctg ctcgacgagc tgtggcgcca
     1741 cgcgacgcag cctcaattca ccttccgcca gcagtggcgc gtgggcgacg tggtggtgtg
     1801 ggacaaccgc tgcacgctgc accggcgcga cgcgtttgat ccgcgcgcgc ggcggctcct
     1861 gcaccgcacc cagatcaggg cgacggctta atccgctttc gcagctcctc gacgcgcgcg
     1921 cggttcacgc cgaagtcgcg ccggccgagg cgcgaggccg agcgcacgtg gatcacgctt
     1981 ccgtcgtagt ggaactcgac gtcgtcgacg tagcccagga gcgccgagcg gaattcggcg
     2041 tagagatagt gcgagtcggc ggagatgacg cgcgcgcgcg gcatcgactc gaccgccttg
     2101 cgcaccgcgg cgatcgcgtc gccgccctgg aacgcaatcg gcgcggtgta atgctcctgg
     2161 tccgccggat cggcctgcga cgacacgcag ttgggcgtgc tgcggcacgg cgccagtcgc
     2221 ccacccatca caccgaggtt cttcggtcgc cgccagtcga acatgttcgc tagctcctag
     2281 tcgacgaagc ggtctgcccg cactgcgagc gacggcggaa tcgcgaagcc gagcgccttt
     2341 gcgaaccgcc ggttcacgac cagctcgaat cgcgccggtc gctcgatcgg catctcaccg
     2401 ggctttgcgc cgcgcaggat gcggtcaaca taggcggccg cgcgccggcc aagctccgaa
     2461 aattccgcgg agtacgcgat cacgccgccc tgcatgaaat agacgccggc agcatcgcag
     2521 gcggtcggaa gctttcgctt cagcacgaaa tccgcaattc gcgagatggt tttgggttga
     2581 gattgtggtc cgccagcgct cacgaaaagc gcgtcgaccc cggagctttc ggccgcagcg
     2641 agattcgcct caagcgaagc ctcatcccgg ttcaaccagg gacgtatctt gatgttcatc
     2701 gccgtggccg cggtctgcat ctcgcctagt gcgaggtccg tttcgacggc cgggaaggcc
     2761 gggggcgcat atccccaaaa gacgccgagc tggcgtgccg tcggtactag ctc
//
LOCUS       DASTPD010000260         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1565359, whole genome shotgun sequence.
ACCESSION   DASTPD010000260 DASTPD010000000
VERSION     DASTPD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565359"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFR57_08600"
     CDS             complement(<1..531)
                     /locus_tag="VFR57_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0259460.1"
                     /translation="MSGFVSLAAVVIVAVLIFLVLFEPTVAYRARRPAARIDSCEFVN
                     YVSAIVNARPLPPAEVRVLNDGEQVFAAELRAIREARRSIHLEVYLLVRGRVADEMLA
                     ALEERARNGVRVRLIIDRYGSLLTPKRYFDTLLAAGGEVRWYQPIAWYTLKRLNNRTH
                     RDLLVVDGAVAFVGGIG"
     gene            complement(547..1329)
                     /locus_tag="VFR57_08605"
     CDS             complement(547..1329)
                     /locus_tag="VFR57_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0259461.1"
                     /translation="MTNQSVLWNVDSRGVATVTLSRPEVNNAYDEALIQGLHHAMDAL
                     GAQAGVRAVVVRGAGRHFQAGADLKWIARVAAQSTDENERASRATALAVHRLNTLPVP
                     TVALVQGGCFGGGTGIVAACDIVIAAEDAMFSIAETRWGLMAGIILPQLVDAIGTRQV
                     RRYALSGERFGAQEARRIGLVHEVVPAGELQNAGERLVGQLLQNAPEANAQTKAVALE
                     FAWGAIDEAALERLVVQHAAKRRSREAAEGLASFREKRPARW"
     gene            1513..2865
                     /gene="nhaA"
                     /locus_tag="VFR57_08610"
     CDS             1513..2865
                     /gene="nhaA"
                     /locus_tag="VFR57_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEU0259462.1"
                     /translation="MTKDARYPLEPLFGRILSPFEQFLRRTTAGGIVLIATTIVALAL
                     AMILGADAIHRFTEQPVGLAAGGRFRLELSLHQWVNDGLMAFFFLLVGLELKREILVG
                     ELSSLKDAALPVIAGLGGMVLPALIYLALNAGTPAASGWGIPMATDIAFAVGILVLLA
                     RRVPKNLIIFLTALAIADDLGAVLVIAVFYTADLNLRALGTAAGLFAILVLFNRGGVR
                     HPLPYAMAGVLLWLAVHESGVHATLTGILLAMAIPARPAHTPAHFERRVAELQDAFRA
                     DRRDDETTDDPLSNQRMASIAEAMEHSAAAVQSPLQRIEHGLTPWVTFAVIPVFALSN
                     AGIDLLQVAWREALTHNVTLGVIGGLVIGKFAGIGLFSWAAVRCGIARLPSGVRWRHL
                     LGAAWLAGIGFTMSLFISQLAFGAPKLVEEAKLGILLGSALSAVIGLVWLYMAGSRSH
                     "
     gene            complement(3072..>3904)
                     /locus_tag="VFR57_08615"
     CDS             complement(3072..>3904)
                     /locus_tag="VFR57_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0259463.1"
                     /translation="MNNVREHVEKHYGRGRVLDSVIAALRETGKDLARLRPEDLAPVD
                     EFHIRGREATVELARRASLKPGSHVLDVGCGLGGSARYLAAEHQCQVTGIDLTQEYVD
                     GANALAGMVGLSDKVAFRQASALEMPFDDGAFDVVWTEHVQMNIADKHAFYREISRVT
                     RPRGALLFHDIFQGTGGPLHYPVPWAEESSISFLAAPDEVRKTLEALGFLIRDWVDKS
                     EPSLQWFVSAIEKLKVSGPPPLGLHLLMGDTARTKFENNIRNLRERRFVVCQAHAEKQ
                     "
BASE COUNT          623 a         1343 c         1205 g          733 t
ORIGIN      
        1 gccgataccg ccgacaaagg ccaccgcgcc gtccacgacg agcaggtcgc ggtgcgtgcg
       61 gttgttgagg cgcttcagcg tgtaccaagc gatgggctgg taccagcgta cctcgcctcc
      121 agcggcgagc agcgtgtcga agtagcgctt cggggtgaga aggctgccgt agcggtcaat
      181 aatcagccgc acgcgcacgc cgttgcgcgc gcgctcctcg agcgccgcga gcatttcatc
      241 ggcaacccgg ccacgcacaa gaagatagac ctcgaggtga atgctgcgcc tcgcttcgcg
      301 gatggcgcgc agctcggcgg cgaagacctg ctcgccgtcg ttcagcaccc gtacttcggc
      361 cggaggtaac ggccgcgcgt tgacgatcgc gctcacgtag ttcacgaact cgcacgaatc
      421 gatgcgcgcg gccggccggc gggcgcgata ggcgaccgtc ggctcgaaca gcacgagaaa
      481 gatcagcacc gccacgatca caaccgcggc cagcgaaacg aacccgctca tggcgtcatg
      541 cagcgcctac catcgtgccg gccgcttctc gcggaacgag gccagccctt ctgcggcttc
      601 gcggctccgg cgcttggccg cgtgctggac gacgagccgc tcgagcgccg cctcgtcgat
      661 cgctccccag gcaaactcga gcgcgactgc cttggtctgc gcgttcgcct cgggcgcgtt
      721 ctgcagtagc tggcccacaa gcctctctcc agcattttga agttccccag cgggcacgac
      781 ttcatggacg aggccgatgc gccgcgcttc ctgcgcgccg aagcgctcgc ccgagagcgc
      841 gtagcggcgc acttggcgcg tgccgatcgc gtcgacgagc tgcggcagga taatgccggc
      901 catcagcccc cagcgcgtct cggcgatcga gaacatcgcg tcctcggccg cgatcacgat
      961 gtcgcacgcg gcgacgatgc cggttccgcc gccaaagcag ccgccctgca ccagcgccac
     1021 ggtgggcaca ggcagcgtgt tcagcctgtg caccgccagc gcggtagcgc gcgaggcgcg
     1081 ctcgttctcg tcggtcgatt gcgcggcgac ccgcgcgatc cacttgaggt ctgccccggc
     1141 ctggaaatgc cggcccgcgc cgcgtaccac cactgcgcgt accccggcct gcgcaccgag
     1201 cgcatccatc gcgtggtgca gcccctggat gagcgcctca tcgtaggcgt tgttgacctc
     1261 gggccggctc agcgtcaccg tcgccacgcc gcgcgaatcc acgttccaca agacgctctg
     1321 gttcgtcatt tctcctcctt cgaataccta taatcgcgcg gtgaacgacg ccgcgcacac
     1381 gcccatgatg cagcagtcct ttccccctac gaaaaatcgg cgggaaaagg acaacctaca
     1441 ccacgaccta tactgcctcg tcacccgctc cgtcctccgc gtcacgccca tggcgcgaaa
     1501 cggttgcagc cggtgacgaa ggacgctcgc tacccactcg agccgctgtt tgggcgcatt
     1561 ctttcgcctt tcgagcagtt ccttcgccgc acgactgcgg gtggcatcgt cctcatcgca
     1621 acgacgatcg tcgcgctcgc cctcgcgatg atcctgggcg ctgatgccat tcatcgattc
     1681 accgaacagc cggtggggct tgcggcaggt ggccgcttca gactcgagct ttccttgcac
     1741 cagtgggtaa acgacgggtt gatggcgttt ttcttcctcc tggtgggtct ggagctcaag
     1801 cgcgagatcc tggtcggcga gctctcgtcc ctcaaggacg cggcgctgcc cgtcatcgca
     1861 ggtcttggcg gcatggtgct accggcgctg atctacttgg cactcaacgc ggggacgcca
     1921 gcagcctccg gctggggtat ccccatggcg accgatattg ccttcgcggt cggcattctc
     1981 gttctccttg cccggcgcgt tcccaagaac ctgatcatct tcctcacggc gctcgcgatc
     2041 gccgacgacc tcggcgcggt gctggtcatc gccgtcttct acacggccga tctcaacctc
     2101 cgggcgctcg gcaccgccgc cgggctgttc gcgatcctgg tgctgttcaa ccgcggcggc
     2161 gtgcgccatc cgctgcccta tgcgatggca ggcgtgcttc tctggctcgc ggtgcacgaa
     2221 tccggcgtgc acgccacgct caccggcatc ctgctcgcca tggcgatccc ggcacggccg
     2281 gcgcatacgc ccgcgcactt cgagcgccgc gtcgcagagc tccaggacgc cttccgcgcc
     2341 gaccgccggg acgatgaaac aaccgacgat ccgctcagca accagcgcat ggcctcgatc
     2401 gcggaggcga tggagcattc ggccgcggca gtgcaaagcc cgttgcagcg catcgagcac
     2461 ggccttacgc cctgggtgac ctttgccgtc attccggtat tcgcgctttc caacgccggc
     2521 atcgacctgc tccaggtagc gtggcgcgag gccttgacgc acaacgtgac cttgggggtc
     2581 atcggcggcc tggtcatcgg caagttcgcc ggcattggcc tgttcagctg ggcggccgtc
     2641 cgctgcggaa tcgcgcgcct gccctccggc gtgcgctggc ggcatctgct cggggcggca
     2701 tggctcgcgg gcatcggctt caccatgtcg ctctttatct cgcaactggc cttcggcgct
     2761 ccgaagctcg tcgaagaagc gaagctcggc atcctcctag gctcggctct gtcggcggta
     2821 atcggcctgg tctggcttta tatggccggg tcgcgttcgc attgacgcgt ggcgggctag
     2881 gctttggcta gtgcttgttg accctgcgta cgcgaccacc tgccccgcgc agcacggcga
     2941 agtcgccggg attggtacgg agaggcggag ttgacgaggt ctaacgcgct aactctggtt
     3001 gagcgcgttg ttcggcgtca cgatcccacc tttgttggca caatattgcg caatcttccg
     3061 gctgcaattc cttactgctt ctccgcgtgc gcttggcaca cgacgaaacg tcgttctcgc
     3121 aggttgcgga tgttgttctc gaacttcgtc cttgccgtat cacccatcag caggtgaagt
     3181 cccagcggcg gcgggccaga caccttcagc ttctcgatag ctgatacaaa ccattggaga
     3241 gaaggttcgc tcttgtccac ccagtcgcgg atgagaaagc cgagggcctc cagagtcttc
     3301 ctcacttcgt cgggtgcggc caggaaactg atggatgact cttctgccca gggcactggg
     3361 taatgcagag ggccgcctgt gccttggaat atgtcgtgga agagcagcgc tcccctgggc
     3421 cgtgtcactc gactgatctc gcggtagaag gcgtgcttgt ctgcgatgtt catctgaacg
     3481 tgctcggtcc agacgacatc gaaggcgcca tcatcgaagg gcatctccaa tgcgctagct
     3541 tgtcggaacg cgactttatc gctcaacccc accatccctg cgagggcgtt ggcgccatcc
     3601 acgtattcct gggtgaggtc aattcccgtg acctggcact ggtgctccgc ggccaggtag
     3661 cgtgcagagc ccccaaggcc acatcccacg tccaagacgt gcgagccggg cttaagcgag
     3721 gcgcggcgtg cgagttcgac cgtggcctcg cgcccgcgga tgtgaaactc atcgaccggt
     3781 gcgaggtcct cgggtctcag ccgagcgaga tcctttcctg tctcccgcag cgcggcgatg
     3841 accgaatcga ggacccgccc tcgaccataa tgtttctcga cgtgctctcg cacattgttc
     3901 atca
//
LOCUS       DASTPD010000261         7768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_340120, whole genome shotgun sequence.
ACCESSION   DASTPD010000261 DASTPD010000000
VERSION     DASTPD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340120"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..867
                     /locus_tag="VFR57_08620"
     CDS             490..867
                     /locus_tag="VFR57_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259464.1"
                     /translation="MVRTAIPLSVIALAVLAGCATEAPVRPAPAPVVLTPTPAPVVVT
                     PAPAASAPVVVAQPTPTAIRPGFGRVESIAAVAPSAAAGGASMRRLGIKMEDGTVQYV
                     DTAAPGYAVGDRIELTRDGYIRR"
     gene            complement(869..1000)
                     /locus_tag="VFR57_08625"
     CDS             complement(869..1000)
                     /locus_tag="VFR57_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259465.1"
                     /translation="MRHLQAARELPNDEDIVARVYGSEDFKEGCAPSRSAHPVGAEK"
     gene            1105..1530
                     /locus_tag="VFR57_08630"
     CDS             1105..1530
                     /locus_tag="VFR57_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259466.1"
                     /translation="MRGDSQAEYVAVPHPLIVGSIPTRRTSAESCALKLGTLVLDGVV
                     ELPMRARAVVSFNYVKAPFTVGYLIKRYRYEARGFMKSVLDASFKYTPSFETDVRKTF
                     HRVRRRQVQTSSRVLVTNSQCELKDAVLTLEQFKKSRAY"
     gene            complement(1560..1763)
                     /locus_tag="VFR57_08635"
     CDS             complement(1560..1763)
                     /locus_tag="VFR57_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259467.1"
                     /translation="MKTVLLLIAAASIAGCGVEMAGTAATAGSIKKRELEQGQKTMKD
                     AQQRIDASMQQVQARQRDDSTQE"
     gene            complement(1812..2120)
                     /locus_tag="VFR57_08640"
     CDS             complement(1812..2120)
                     /locus_tag="VFR57_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259468.1"
                     /translation="MHVTDRRKNRLGLKVIHKHRIGDKVFRRVSELVLIDGQPKALLG
                     WIDMGGVRTPIYLCDLDHRKLHRSASSPNTYYYEEITVDPRFEAYGPSALGGDQASPS
                     "
     gene            complement(2195..2935)
                     /locus_tag="VFR57_08645"
     CDS             complement(2195..2935)
                     /locus_tag="VFR57_08645"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEU0259469.1"
                     /translation="MRISAPFGYKEIVPFLKTQKVRLLAAGEVPEFIQRGNAVPISHS
                     EFQPVARHYPIVFASGDAKTYAAVAVLGFTAGENLFCRDGKWKSGAYIPAYARRYPFC
                     MARVNLNEVEQKDRLICVEKSAIDEAGGEAMFDAAGQPTEKWKAIERLLGEYEADLER
                     AREMCATLAEYGLLEAFNMQATLTKERGGRSMQLTGMHRVVEQNLENLNAAQLKNLVR
                     KGYLARIYMHLLSLQNFGRLLDRNGRTA"
     gene            2975..3050
                     /locus_tag="VFR57_08650"
     tRNA            2975..3050
                     /locus_tag="VFR57_08650"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3008..3010,aa:Ala,seq:cgc)
     gene            complement(3115..3621)
                     /locus_tag="VFR57_08655"
     CDS             complement(3115..3621)
                     /locus_tag="VFR57_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259470.1"
                     /translation="MRAAATALLLALAVVVSESALARKGGGGHVGGAKTYGGHVSGGK
                     VYGGHASGGKVYGGHVGHGQSHFKGHHHHGHHHSTTFVGVGFGFAYPWSWYGYYPPPY
                     YYPGYYYPQPVAYPAEPVTYVEQSAPAQTEPAGWWYYCETSRTYYPYVKECPNGWQRV
                     PALPPPPN"
     gene            complement(3659..4834)
                     /locus_tag="VFR57_08660"
     CDS             complement(3659..4834)
                     /locus_tag="VFR57_08660"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEU0259471.1"
                     /translation="MARSSTIAGITALAGLLALALAQGIGRFAFTPILPMMQEDTGLS
                     LADGGRLAAANYVGYLIGALWAMVQPARTDHAVRASLAITAAATVAMAFANDMLAYLA
                     LRALAGVSSAWVLIHVSSWCASRLAVLGRPALNGVVFAGVGAGIVFAGALCIALMTLG
                     AGSRMAWLLLGVAGFLVTATVWPVVKEVGSATKVPFTRYRWTPDALRLVACYAAFGIG
                     YIIPATFVPVMAKAVIQNPAIFGWAWPVFGAAAVASTLMAGAFARRLNGRRLWMASTC
                     VMALGVASALFVQGLTGIIGCALLVGGTFVVATLAGVQVAREVASGGAYVLVAAMTAS
                     FAASQVAGPLIVSFLAERSLGFDAALWSALIALLASAGALMLPLRQTTTEKSGRCNN"
     gene            complement(4844..5173)
                     /locus_tag="VFR57_08665"
     CDS             complement(4844..5173)
                     /locus_tag="VFR57_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259472.1"
                     /translation="MIRSYAVAALLSFTLNAWADGPGTRIRDTPLPSRPAAAVDRDLQ
                     RCEAMRGDEKERCLRALRDAARRDERAPHAGPGPERGPESIGMGSGAGSTGPGVGSGT
                     TGGAGAR"
     gene            complement(5477..6562)
                     /locus_tag="VFR57_08670"
     CDS             complement(5477..6562)
                     /locus_tag="VFR57_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEU0259473.1"
                     /translation="MRTSYTDAAGSARAAGLQYGSDAAAGIARRRAGNAFSYQLNGRA
                     VRERETLARIKSLAIPPAWEQVWINPREDGHLQATGRDARGRKQYRYHRRWREVRDET
                     KYGRMLAFAKALPRIRRRVGQDLALPGLPRDKVLATVVRLLETTRMRVGNDEYARENE
                     SYGLTTLRTRQVRVNGTKLEFRFRGKSGVWHQFALHDRSLAAIVRRMRDLPGYELFQY
                     VDEDGETRAVDSADVNAYLKSIAGEEFTSKDFRTWAGTVLAAEALRECGPKNGLSHAI
                     ERVARQLGNTKAVCRKCYIHPAVIDAYVDGTLAEAMTSRSVETCVVRLLARGRRQRSL
                     VPALKKSLSRLRRRATAKGASHHAQAA"
     gene            6747..>7768
                     /locus_tag="VFR57_08675"
     CDS             6747..>7768
                     /locus_tag="VFR57_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU0259474.1"
                     /translation="MMPLRPMAALLQRLVDKIDPHEGRYRMLFERSPLPMWVVDTQTL
                     RFLAVNDAGLRLYEYSRNEFLAMTASEISRGEEIPFRDFLRREGGEVLHGVFRHAKKT
                     GETIDIEGVGHLVAWRGRPARLVQINDITERLRTQQTLERLNRELEVSHERLRALSRR
                     LFEVQEEERKRLARDLHDDVGQALTALKIQLESIARPGNELTARQRIEEIVDTVQHTL
                     ERVRQLSLSLRPPQLDDLGLAAALRSHLDRQARVAGLHAHFEAEDAPQELAPDAETAC
                     FRVAQEAINNVLRHARARNLWVRLFTAGGRLAISVRDDGRGFDLDSVRARASNGASLG
                     LVGMEER"
BASE COUNT         1329 a         2489 c         2570 g         1380 t
ORIGIN      
        1 gagcgcagga tgttcatgga tttaatcctt aaggtgatgt gatcctgcag caacttgcat
       61 accggatcgc gagggggctg gatctgacca gggtatcgag ttcatcagcg caactgctcc
      121 aatcagcgga ctgcgcgata gaagtgcagc tgcgggagtg cgctcccacg aagaacaaag
      181 acaccacagc gataggtaga cgtgcttgcc ggccagacga tgccagctgg gaatgcccgc
      241 gccctgcgtt tgcagctgcg gcggcctcac ttccgtgatt ggtctaccgc gtcgaactct
      301 catgtagtgg gtgagtaaga acgcttttgc ggttaagcca accagcgcaa gatctcggcg
      361 acaaactgat agtccgaccg ctgattggcc aagctacggt caatcatggc ccgctggcca
      421 ggtaggtaat tcgggtcaga ccccggtatg cagattgctg cttcctgtag tcctttacag
      481 gaggtattca tggtcagaac cgctattccc ctttccgtga ttgcacttgc ggtgctcgcg
      541 ggctgcgcca ccgaagcgcc ggtgcggccg gcgccggcgc cggtcgtact gacgccgacg
      601 ccggctcccg tcgtggtgac acccgcgccg gcggcgagcg cgccggtggt ggtcgcccag
      661 cctacgccga ccgcaatccg tccgggcttc ggtcgcgtcg agtcgattgc cgcagtggcg
      721 ccgtcagccg ccgcgggcgg tgcgagcatg cgccgcttgg gcatcaagat ggaagacggc
      781 accgtgcagt acgtcgacac ggccgcgccg ggctacgcgg tgggcgaccg catcgagctg
      841 acgcgcgacg gctacatccg ccgctaggct acttttccgc tccaacgggg tgcgcgcttc
      901 tcgaaggcgc gcacccttcc ttgaagtcct cgctgccgta gacgcgcgcg acgatgtcct
      961 cgtcgttggg cagctcccgc gcggcctgca ggtgacgcac tggtggccca caccctgatc
     1021 ggcgcgtgcg aggtccatct tgtcggcgat ctcgcgcacg cgcacgaaga tgactccgat
     1081 tccatggagt gaatactgtt ccgcgtgcgt ggcgactccc aagcagaata cgtggccgtg
     1141 cctcatcctt tgatcgttgg ttcgattcca acccggcgca cttctgcaga aagctgtgcg
     1201 ttgaaacttg gtacgttggt actagatggg gtagttgaat tacccatgcg agcgcgtgcg
     1261 gtcgtctcat ttaactatgt caaggctccc tttaccgtcg gttatctcat caagcggtac
     1321 cgctacgagg caaggggttt tatgaaaagc gtcttggatg catccttcaa atacacacca
     1381 agcttcgaaa ccgatgtgcg caagactttt cacagagtcc gccgtcgaca agtacagacg
     1441 tccagccgcg tgctagtgac gaatagccaa tgcgagttga aggatgcggt gctgacgctg
     1501 gaacagttta agaagtcgcg cgcctactag ggtagtcgag caagtttcgc tggccgactc
     1561 tattcctggg tcgaatcgtc gcgctggcgc gcttgtactt gctgcatgct ggcatcaatt
     1621 ctctgctgcg cgtccttcat cgtcttttgc ccctgctcaa gttcgcgttt cttgatcgag
     1681 ccagcagtcg cggcggtacc ggccatctcc acgccgcagc ccgcgatgct tgcagcggcg
     1741 atcagcaaca gaacggtttt catgcgaggc aatatagacg cagacgacgc gccctgcccc
     1801 ggcataatgt ctcaggaagg cgacgcctgg tcgccgccga gcgcgctcgg accataggcc
     1861 tcgaagcgcg ggtccacggt gatttcctcg tagtagtagg tgttcgggct cgacgcggag
     1921 cggtgaagct ttcgatgatc gagatcacag aggtaaatcg gggtccgtac gccgcccatg
     1981 tcgatccagc cgaggagggc cttgggctgg ccgtcgatca gcaccagctc ggacacgcgg
     2041 cgaaaaacct tgtcgccgat ccggtgcttg tggatcacct tgaggccgag gcgatttttc
     2101 ctgcgatcgg tcacgtgcat accctgcaaa ggcctgctga gcaggagtcg tgcccggcgc
     2161 tgtggcgcgg tgacaagctg tcatcgtaaa gcgtctacgc ggtgcggccg ttgcggtcga
     2221 gaagacgccc gaagttctgc agcgagagga ggtgcatgta gatgcgcgcg aggtagccct
     2281 tgcgcacgag gttcttgagc tgcgcggcgt tcaggttctc gaggttctgc tcgaccacac
     2341 ggtgcatgcc ggtgagctgc atagaacggc cgcccctttc cttcgtcaac gtcgcctgca
     2401 tattgaaagc ctcgagcagg ccgtattcgg cgagtgttgc gcacatctcg cgcgcgcgct
     2461 cgagatcggc ttcgtattcg ccgaggaggc gctcgatcgc tttccatttt tccgtcggct
     2521 gcccggctgc gtcgaacatg gcctcgccgc ccgcctcgtc gatggcggac ttttcgacgc
     2581 agatgagccg gtctttctgc tccacctcgt tcagattcac gcgcgccatg cagaacggat
     2641 aacggcgcgc gtaagccgga atgtaggcgc cgcttttcca tttgccgtcg cggcagaaga
     2701 ggttctcgcc cgcggtaaac ccgagcaccg cgacggcggc gtaggtctta gcgtcgccgc
     2761 tcgcgaagac gatgggatag tggcgtgcga ccggctggaa ctcgctgtgg ctgatcggca
     2821 ccgcgttgcc gcgctggatg aattccggga cttctcccgc agcgagcaaa cgcactttct
     2881 gcgtcttcag gaacggtacg atctccttgt agccgaaggg cgcggagatc cgcatcgcgc
     2941 aggcatatta cggtaaaatc ccgcgcctca cgccggggtg gtagctcagc tgggagagcg
     3001 tcgcgttcgc aatgcgaagg tcgggagttc gatcctcctc cactccacca ttacatagcc
     3061 tgtcgcctca gagcccagca cgacaagtag tgcgacggtc gcaattcaca tgcttcagtt
     3121 aggcgggggc ggcagcgctg gcacgcgctg ccagccattg ggacactctt tcacgtaggg
     3181 gtagtaggtc ctggacgttt cgcagtaata ccaccagccg gctggctcgg tttgcgccgg
     3241 agcgctttgc tcgacatacg tgaccggctc ggcagggtat gcgaccggct gcggatagta
     3301 gtagccagga tagtaatagg gcggcgggta gtagccgtac caggaccacg gataagcgaa
     3361 tccgaagccg acaccgacga acgttgtgct gtggtgatgg ccgtgatgat ggtggccctt
     3421 gaagtggctt tggccatggc cgacatgccc gccgtagacc ttgccgccgc tggcatgccc
     3481 tccatagacc ttgccgccac tgacatgccc gccgtaggtc ttggcgccgc cgacatgtcc
     3541 gccgccgccc ttgcgcgcaa gcgccgattc gctaacaact actgcgagcg caagaagtag
     3601 tgctgtggcc gccgcacgca tcgctttagg ctacacccgt cggcgcgcaa gagggtaact
     3661 agttgttaca ccggccgctt ttctcagtgg ttgtctggcg taacgggagc atcagtgcgc
     3721 cggcagaagc aagaagcgcg atgagcgcgc tccacagcgc ggcgtcgaag ccgagcgagc
     3781 gctctgccag gaagctgacg atcaacggac ctgcgacctg actcgccgcg aaggaagccg
     3841 tcatcgccgc gacgagcaca taagcgccgc cgctggccac ttcgcgcgca acctggacgc
     3901 ctgcgagcgt tgcaacgacg aaggtgccgc cgacgagaag cgcgcagccg atgattcccg
     3961 tcaggccttg gacgaaaagc gcgctggcga cgccaagagc catgacgcac gtgctcgcca
     4021 tccacaggcg acgaccgttc aggcggcgtg caaaggcgcc ggccataagc gtggatgcga
     4081 ctgcggccgc gccgaacacc ggccatgccc atccgaagat tgccgggttc tggatcaccg
     4141 ccttcgccat gaccggcacg aacgtcgccg gaatgatgta gccgatgccg aaagcggcgt
     4201 agcacgcgac gaggcgaagt gcgtcgggcg tccagcgata gcgggtgaaa ggaaccttgg
     4261 tcgcgctgcc cacttccttg accaccggcc ataccgtagc ggtgacgagg aaaccggcga
     4321 cgcccaggag cagccacgcc atgcgtgagc cggcgccgag cgtcatgagc gcgatgcaca
     4381 gagcgccggc gaagacgatc ccggcgccga cgccggcaaa caccacccca ttcagcgccg
     4441 gacggccgag aaccgcaagg cgcgacgcgc accatgagct cacgtgaatc agcacccagg
     4501 cgctcgatac cccggcgagc gcgcggagtg ccaggtaagc gagcatgtcg ttggcgaatg
     4561 ccatcgccac agtcgcggcc gcagtgatgg cgagtgacgc gcgcactgca tggtcggttc
     4621 gcgccggctg caccatcgcc cagagcgcgc ctatgaggta gcccacatag ttcgccgcgg
     4681 cgagccggcc gccatcggcg agcgagaggc cggtgtcctc ctgcatcatc ggcaggatcg
     4741 gcgtgaaagc gaagcggccg attccctgcg cgagcgcaag cgcgagcagc ccggcaaggg
     4801 ctgtgatccc cgcgatggtt gagcttcgag cgatgaacga agactaacgc gcgccggcgc
     4861 cgccggtcgt gccgctgccg acgcccggac cggtgcttcc tgcgccggaa cccattccga
     4921 tcgactccgg tccacgctcg ggcccggggc ctgcgtgcgg cgctcgctcg tcccggcgtg
     4981 ccgcatcgcg cagcgcgcgc aggcagcgct ccttctcatc gccgcgcatg gcttcgcagc
     5041 gctgcagatc cctgtcgacc gccgcggcgg gtcgcgacgg gagcggcgtg tcgcggatcc
     5101 gcgtgccggg gccgtcggcc cacgcgttca aagtgaagct caagagagcg gcgacggcgt
     5161 aggacctgat catcagcgca ccgtgccggt cggcggagtg gtttgtggcc aagtggttcc
     5221 aggcggctgg gtgctcccgc cagtggcagc attgccggcc tggaggcatt tgtccctttc
     5281 ggcgccgatc aagttttcgc acgggttgag tccgcccacc ggcctggagc cgagagccgg
     5341 cggcgggcca ccgagggtcg gaggtgttgc cggcgtcact gtgctcgggt tctgggtcgg
     5401 cgggacgctc ggcgtagctt gatccgggtg ggtctgcgcg agcgcggtgg ctgcagcaag
     5461 gaacgacaag gcggcgctaa gcggcttgag catgatggga agctcctttg gccgtggcgc
     5521 ggcggcgcag gcgggagagg gatttcttga gggcaggcac gagcgaacgc tgacggcgcc
     5581 cgcgcgcgag caggcgcacg acgcacgtct cgacgctgcg gctcgtcatc gcctccgcca
     5641 gggtgccgtc gacgtaagca tcgatcaccg cagggtggat gtagcacttg cggcacaccg
     5701 ccttggtgtt gccaagctgg cgcgctacgc gttcgatggc gtgcgacagg ccattcttcg
     5761 gtccgcactc acgcaacgct tcggcggcta ggacggtgcc cgcccaggtg cggaagtcct
     5821 tgctcgtgaa ctcttcgccg gcgatgctct tcaggtaggc attgacgtcg gccgaatcga
     5881 ccgcgcgagt ctcgccgtcc tcgtcgacgt actggaaaag ctcgtagccg gggaggtcgc
     5941 gcatgcggcg cacgatcgcc gcgaggctgc gatcgtgcag cgcaaactgg tgccacacgc
     6001 cgctcttgcc gcggaagcgg aactccagct tcgtgccgtt cacgcgcacc tggcgtgtgc
     6061 gcagcgtggt gagcccgtag gattcgttct cgcgcgcgta ctcgtcgtta cccacgcgca
     6121 tgcgcgtggt ctccagcaag cgcaccacgg tggcgagtac cttgtcgcgc ggcagaccgg
     6181 gcagcgccag gtcctggcct acgcgacgac ggatgcgcgg cagcgccttt gcaaacgcaa
     6241 gcatgcggcc gtacttcgtc tcgtcgcgta cctcgcgcca gcggcggtga tatcgatatt
     6301 gcttgcggcc gcgcgcgtcg cgccccgtcg cctgcagatg gccgtcctcg cgcgggttaa
     6361 tccagacctg ctcccaagcc ggcgggatgg cgagtgactt gattcgcgcc aacgtttcgc
     6421 gctcgcgcac ggccctgccg ttcagctgat aactaaaggc gttgccggca cggcggcgcg
     6481 cgatgcccgc ggctgcgtcg ctcccgtact gcagccccgc ggcccgggca gagccggctg
     6541 cgtccgtgta agaagttctc aggggaaggt tcctcggcgt cctttcttca tgctgcgatc
     6601 aagttagcct tcggccggac ccccggttgg aggcggcgcg cttgtgcggc agccgcctgc
     6661 tcccggtccg tatccgggtg cttgcgtttt ttgcgtcatg ccggcgctac ccgcaaacta
     6721 catgccacga ttaccattcg cgctcgatga tgccgcttcg cccgatggcc gcgctcctgc
     6781 aacggctggt cgacaagatc gacccgcacg aagggcgtta tcgcatgctc ttcgagcgta
     6841 gcccgctgcc gatgtgggtg gtcgatacac agacactgcg tttccttgcc gtgaacgacg
     6901 ctggcttgcg cctctacgaa tattcccgca atgaatttct cgccatgacg gcttccgaga
     6961 tcagccgtgg cgaggagatt ccgttccgcg atttcctgcg ccgcgaaggc ggtgaagtgc
     7021 tgcatggcgt gttccggcac gcaaaaaaaa ccggcgagac gatcgacatc gagggcgtgg
     7081 gacacctggt cgcgtggcgc ggccgtccgg cgcgcctggt gcagatcaac gacatcaccg
     7141 agcgcctgcg cacccagcag acgctcgagc ggctgaaccg cgagctcgag gtttcgcacg
     7201 agcggctgcg cgcgctgtcg cgtcgcctgt tcgaggtgca ggaagaggaa cgcaagcgcc
     7261 tggcgcgcga cctgcacgac gacgtgggac aagccctcac cgcgctgaag atccagctgg
     7321 agtcgatcgc gcggccgggc aacgagctca ccgcgcgcca gcgcatcgaa gaaatcgtcg
     7381 acacggtgca gcacacgctc gagcgcgtgc gccagctttc gctgagcctg cggccgccgc
     7441 agctcgacga cctcggcctg gccgcggcgc tgcgctcgca cctcgatcgc caggcgcgcg
     7501 tcgccgggct gcacgcgcac ttcgaggccg aggatgcgcc gcaggagctc gcgcccgacg
     7561 ccgagaccgc ctgcttccgc gtggcgcagg aagcgatcaa caacgtgctg cgccatgcgc
     7621 gcgcgagaaa tctgtgggtg cgcctgttca ccgccggcgg acgcctggcg atctcggtgc
     7681 gcgacgacgg ccgcggcttc gacctggatt cggtgcgggc gcgcgcgtcg aacggcgcga
     7741 gcctcggact ggtcggcatg gaggagcg
//
LOCUS       DASTPD010000262         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_340418, whole genome shotgun sequence.
ACCESSION   DASTPD010000262 DASTPD010000000
VERSION     DASTPD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340418"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1022)
                     /locus_tag="VFR57_08680"
     CDS             complement(66..1022)
                     /locus_tag="VFR57_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259475.1"
                     /translation="MLQRLLPLSWLPLLATAIAFGTLPLWIRTVGLYPYLGVEVMIWV
                     IYALGFNLLLGTAGLPSFGHGAYFGVGAYAFGLTQFNVSSDIWVCLAAAIAAGALAGG
                     LVALFISHRRGIYYALLTIAFGQIFWFVAIKWHSVTRGEDGLLNIKRPAFLADLSSLY
                     YFCFAIFIIVILFLWRLQRSPYGRVLAAIRQNETRARFVGYHVWTYKWLAFTLSALVA
                     GLAGGLFALAQQSAYPNVMSLHQSGFVVMMALIGGGLVSFWGPVLGVVLFFVARDLLG
                     AYTETWLLWYGLIFMAMVIFKPEGIAGLWRDGMGRWRAKNGV"
     gene            1067..1900
                     /locus_tag="VFR57_08685"
     CDS             1067..1900
                     /locus_tag="VFR57_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU0259476.1"
                     /translation="MAAARSYEELKGSLSRLYPGMSPQLQRIATFALEHPQDMALDTV
                     ATLARGVGVQPSAMVRFAQALGYDGFSDLQRVFRGRLVERSNHSPGLRNYRERIEAFR
                     ADQPRSGPAGVLAQFVADGIASLEQLHEHVAPADMAAAVRDISAARTIYVLGHRRSFP
                     VAYYLAYALTQLELRTQLLDSVGGMLRDPAQLIGGKDALIVVSFKNYTPEVIEVAQGC
                     HARKVPVVAITDNALSPLARHAKVCFQIEEKTSKPFRMLVAPMCLAQALVVAVGEHIA
                     R"
     gene            1998..>2293
                     /locus_tag="VFR57_08690"
     CDS             1998..>2293
                     /locus_tag="VFR57_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0259477.1"
                     /translation="MKYDLICMGRAAVDLYGEQIGARLEDQTSFAKYLGGCPANIAVG
                     SSRLGLKVAMLTRVGDEHNGRFVRETLAAEGVDVSHVATDPKRLTALVFLSIKDR"
BASE COUNT          449 a          778 c          724 g          342 t
ORIGIN      
        1 tcacgtcctg caacaccacg cgcgacccga agcgcttcga caacccctga accgagaaaa
       61 tggtgtcaga caccattttt cgccctccac ctgcccatgc cgtcgcgcca cagaccggcg
      121 attccctcgg gcttgaagat caccatcgcc atgaagatga gcccgtacca gaggagccag
      181 gtctcggtat aggcaccgag gagatcgcgc gcgacgaaga aaagcaccac gccaagcacc
      241 ggcccccaga acgacacgag ccccccaccg atcagcgcca tcatcaccac gaagcccgac
      301 tggtgcaggc tcatcacgtt gggatacgcg gactgctgcg caagcgcgaa tagccccccg
      361 gcaagcccgg cgaccagcgc cgagagagtg aacgcgagcc atttgtaggt ccagacgtgg
      421 tagccgacaa agcgcgcgcg cgtctcgttc tgccggatcg cggcgagcac gcggccgtag
      481 ggtgagcgct gcagccgcca gaggaagagg atcacgatga tgaagatggc gaagcagaag
      541 tagtagagcg agctcaagtc ggcgaggaac gccggccgct tgatgttgag gagcccgtcc
      601 tcgccgcgcg tcacgctgtg ccacttgatg gcgacgaacc agaagatctg gccgaaggcg
      661 atggtgagga gcgcgtagta gatgccccgg cggtgcgaaa tgaagagcgc gaccagacct
      721 ccggcgagcg ccccggccgc gattgctgcc gcaagacaaa cccaaatatc cgaagaaaca
      781 ttgaactgcg tcagtccgaa cgcatacgcg ccgacgccga agtacgcccc gtggccgaag
      841 gagggaagcc cggcggtacc gagaagcagg ttgaagccga gcgcgtagat cacccagatc
      901 atcacctcga ccccgaggta gggatacagg ccgaccgtcc tgatccagag cggcaaggtg
      961 ccgaacgcga tcgcggtcgc gaggagagga agccacgaca ggggaagaag ccgttgcaac
     1021 atttgttcta cagttgtatt gttctggaac atacgttcta aagtcaatgg ccgccgcccg
     1081 cagttatgaa gagctgaagg gaagcctgtc ccggctctac cccgggatgt cgccgcagct
     1141 ccagcgcatc gcgactttcg cgcttgagca cccgcaggac atggcgctcg acaccgtggc
     1201 cacgcttgcg cgcggtgtcg gcgtgcagcc ctcggcgatg gtgcgcttcg cgcaggcgct
     1261 cggctacgac ggcttctcgg acctgcagcg cgtgttccgc ggccgcctgg tcgagcgctc
     1321 caatcactcc cccggcctgc gcaactaccg cgagcgcatc gaggcgttcc gcgccgacca
     1381 gccgcgctcg ggcccggccg gggtgctcgc acagttcgtc gccgacggca ttgcctcgct
     1441 cgagcagctg cacgagcacg ttgcgcccgc agacatggcg gccgcggtgc gcgacatctc
     1501 ggcggcaaga acgatctacg tcctcggcca ccgccgctcc tttccggtcg cctactacct
     1561 cgcctacgcg ctcacgcagc tcgagctgcg cacccagctc ctcgactccg tgggcggcat
     1621 gctgcgcgac ccggcgcagc tcatcggtgg gaaggatgcg ctgatcgtcg tctcgttcaa
     1681 gaactacacg cccgaggtca tcgaggtcgc gcaaggctgc cacgcgcgca aggtgccggt
     1741 ggtcgcgatc accgacaacg cgctctcgcc gctcgcgcgc cacgccaagg tctgcttcca
     1801 gatcgaggag aagacctcca agcccttccg catgctggtc gcgcccatgt gcctcgcgca
     1861 ggcgctcgtg gtagccgtcg gggagcacat cgccagatga gcgcgccggt tttttgcccc
     1921 cctgacaagg ggggagcgag cgaagcgagc ggggggttcg tttactttct gctcacaaaa
     1981 ccaacccccc gctcgccatg aaatacgacc ttatctgcat gggccgcgcg gcggtcgacc
     2041 tctacggcga gcagatcggc gcgcggctgg aggatcaaac gagcttcgcc aagtacctcg
     2101 gcggctgccc ggcaaacatt gcggtgggct cgagccgcct cgggctcaag gtcgcgatgc
     2161 tgacgcgcgt cggcgacgag cataacggcc gcttcgtgcg cgagacgctt gccgccgagg
     2221 gcgtcgacgt atcgcacgtc gccaccgatc cgaagcggct taccgcgctc gtattcctct
     2281 ccatcaagga ccg
//
LOCUS       DASTPD010000263         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1362113, whole genome shotgun sequence.
ACCESSION   DASTPD010000263 DASTPD010000000
VERSION     DASTPD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362113"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFR57_08695"
     CDS             complement(<1..563)
                     /locus_tag="VFR57_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEU0259478.1"
                     /translation="MNAQLILEDGTVFRGRSLGAGRSVSGEVVFNTGMVGYTEALTDP
                     SYRGQIMTLTFPLVGNYGVPRDFESPRIQASALVVSELATEYSHSAAERSLPQWLKDE
                     GIPCIAGIDTRALTKRLRTRGCMLGKVVVTEDVPFEDPNARNLVAEVSTDHREVFLGS
                     GKTVVLVDCGAKGSIVDELRKRDFTVIR"
     gene            complement(560..1609)
                     /locus_tag="VFR57_08700"
     CDS             complement(560..1609)
                     /locus_tag="VFR57_08700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0259479.1"
                     /translation="MRRFLTSAALMAAGFAVALALGEAVVRFALKDQTVMFPRYHTDY
                     RYGEYTLRGIRPNAHFRHTSVDGSWEFTTNSKGLRDARDFPYPKPAGTLRVLALGDSH
                     TQGYEVAQDATFSAVLERYLNSHGKKAEVLNAGVSGYGTAEAAAYLEAEGIKYQPDVV
                     VLGFFANDYEDNLKAGLFGLEDGKLVARSREHIPGVRIQNLIYAVPGVTWLSENSYFY
                     SVAFNTVWLHSKNLLTAHAKEAAVDYAVATKSQSKYDVELAAALVERMRSFCESKGVR
                     FIVVDIPEAPAPYRIKSSVAFPTRGLELVSSDSLLKPYEGAAVFHRPHGHHHITEFTH
                     MLIGVELGKRILEKK"
     gene            complement(1731..>2583)
                     /locus_tag="VFR57_08705"
     CDS             complement(1731..>2583)
                     /locus_tag="VFR57_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259480.1"
                     /translation="YNDIYSKNGKWFMDGIGGAENFSDAGFPFADSDIYGNRISQVYD
                     DGIEAEGANRNVRIWKNWFDQVFVAIGNAATTTGPLYVWRNVSNRMANVYQPEGDPDA
                     ELRGPFIKAGSNHARFNGGRAYYFHNTILQPPAKRYGGGAGWGIYNSGGKLTNFVSRN
                     NVWHIHKEVIIHGQPKFYAISANEQGADADFDLHNGSLNRAGSSAERRGWMSTPVYAS
                     SGKNYPDLGAQPGNFQLRKGSPGYGAAEPIANFNDGFGARPDVGAHQSGQPPMRFGRE
                     AALSSVD"
BASE COUNT          393 a          829 c          885 g          476 t
ORIGIN      
        1 cggatcaccg tgaagtcgcg cttgcgcagc tcgtcgacga tgctgccctt cgcgccgcag
       61 tcgaccagca ccacggtctt gccgctgccg aggaacacct cgcggtgatc ggtgctcacc
      121 tccgcaacca ggtttctcgc gttcggatct tcaaagggaa catcttctgt aacaacaacc
      181 tttccgagca tgcagccgcg ggtgcgcagg cgcttggtga gcgcgcgcgt gtcgatgccg
      241 gcgatgcagg ggatgccctc gtccttcagc cattgcggca ggctgcgctc cgcggcgctg
      301 tggctgtact cggtggcgag ctcgctcacc accagcgcgc tcgcctggat gcgcggcgac
      361 tcgaagtcgc gcggcacgcc gtagttgccg accagcggga aggtgagggt catgatctgg
      421 ccgcggtagg acggatcggt gagcgcctcc gtgtagccga ccatgccggt gttgaacacc
      481 acttcgcccg acaccgagcg cccggcgccg aggctgcggc cgcggaatac cgtgccgtct
      541 tcgaggatga gttgcgcgtt cattttttct ccaagattcg cttgcccagc tccacgccga
      601 tcagcatgtg cgtgaactcg gtaatgtggt gatggccgtg cgggcggtgg aacacggccg
      661 cgccttcgta cggcttgagc aggctgtcgc tcgagacgag ctcgaggccc cgggtcggaa
      721 acgccaccga cgatttgatc cggtacggcg cgggcgcctc gggaatgtcc accacgatga
      781 agcggacgcc cttcgactcg cagaagctgc gcatgcgctc caccagcgcg gcggcgagct
      841 cgacgtcgta cttcgactgg ctcttggtgg cgaccgcgta gtccaccgcg gcttccttgg
      901 cgtgcgcggt gagcaggttc ttggaatgca gccacacggt gttgaacgca acggagtaga
      961 aatacgagtt ctccgacagc cacgtcacgc ccgggactgc atagatgagg ttctggatgc
     1021 gcacgccggg aatgtgctcg cggctgcgcg cgaccagctt gccgtcctcg aggccgaaca
     1081 ggccggcctt gaggttgtcc tcgtaatcgt tggcgaagaa gccgagcacc accacgtcgg
     1141 gctggtactt gatgccctcg gcttccaggt acgcggcggc ctcggcggtg ccgtagccgg
     1201 agacgccggc gttcaggact tccgctttct tcccgtgcga gttcagatag cgctcgagca
     1261 cggcagagaa cgtcgcgtcc tgcgcgactt cgtagccttg cgtgtgcgag tcgccgagcg
     1321 cgagcacgcg cagcgtgccg gccggcttcg ggtacggaaa atcgcgcgcg tcgcgcaggc
     1381 ccttgctgtt ggtggtgaac tcccacgagc cgtccacgct ggtgtggcgg aagtgcgcgt
     1441 tggggcggat gccgcgcagc gtgtactcgc cgtagcggta gtcggtgtgg tagcgcggga
     1501 acatcaccgt ctggtccttc agcgcgaagc gcaccacggc ctcgccgagg gcgagcgcga
     1561 ccgcgaagcc cgcggccatc agcgccgcgg aggtcaggaa gcgtctcatc aagcgaccaa
     1621 aatagcgcgc gacataggag aactcggcgc agccgcagcg ccccgaacgg actatggctt
     1681 ttctgctgtc gtccccgcgc aggcggggac cccgttttat tttctacaaa ctaatcgaca
     1741 gacgataaag cggcctcgcg cccgaagcgc atcggcggct gcccggactg gtgcgcgccc
     1801 acgtcggggc gcgcgccgaa gccgtcgttg aagttcgcga tcggctcggc cgcgccgtag
     1861 cccgggctgc ccttcctcag ctggaagttg ccgggctgcg cgccgaggtc cgggtaattc
     1921 ttgccgctcg aggcgtacac cggcgtggac atccagccgc gccgctcggc gctcgagccg
     1981 gcgcggttga gcgagccgtt gtgcaggtcg aagtcggcgt ccgcgccctg ctcgttcgcg
     2041 ctgatggcgt agaacttggg ctggccgtgg atgatgactt ccttgtggat gtgccagacg
     2101 ttgttgcggc tcacgaagtt ggtgagcttg ccgcccgagt tgtagatgcc ccagccggcg
     2161 ccgccgccgt agcgcttcgc gggcggctgc aggatggtgt tgtggaagta gtaggcgcgc
     2221 ccgccgttga accgggcgtg gttcgagccc gccttgatga acggcccgcg cagctcggcg
     2281 tccgggtcgc cttcgggctg gtacacgttc gccatgcggt tgctcacgtt gcgccacacg
     2341 tagaggggcc cggtggtggt cgccgcgttg ccgatcgcga cgaacacctg gtcgaaccag
     2401 ttcttccaga tgcgcacgtt gcggttcgcg ccctccgcct cgatgccgtc gtcgtagacc
     2461 tgcgagatcc tgttgccgta aatgtcggag tcggcgaacg ggaagcccgc gtcgctgaag
     2521 ttctcggcgc cgccgatgcc gtccatgaac cacttgccgt tcttcgagta gatgtcgttg
     2581 tag
//
LOCUS       DASTPD010000264         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_751671, whole genome shotgun sequence.
ACCESSION   DASTPD010000264 DASTPD010000000
VERSION     DASTPD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751671"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFR57_08710"
     CDS             complement(<1..202)
                     /locus_tag="VFR57_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259481.1"
                     /translation="MKQVVAALAGAVLLVSCATTREQSLVERAVEAMGGYERLAGIKT
                     FTFKGNAKYWEPEQSEVPGGDMR"
     gene            complement(261..590)
                     /locus_tag="VFR57_08715"
     CDS             complement(261..590)
                     /locus_tag="VFR57_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEU0259482.1"
                     /translation="MVRLSGFEWLALAAALLLCPGGSPTGAGPSATSGASAPARSLFD
                     RLDRNRDGYLSSAELASDEARQANWIAVDRDRDGRIGRGEFGLVDTAKAPPNAAAGGT
                     QAPKKPE"
     gene            complement(647..1267)
                     /locus_tag="VFR57_08720"
     CDS             complement(647..1267)
                     /locus_tag="VFR57_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0259483.1"
                     /translation="MSVALRIVALVTGILAACSSAVAQVQDEAVILVAHPAFRDLEYR
                     QTVLIAAPAPNGGHVGVILNRPTRRSLGSLFPEHEPSKRVADPVYYGGPFSRGALVAL
                     VKADNAPGTGSVLLMKSLYLAFRANTIDHVIETTPNEARYFVGYVGWRPGELKSEIER
                     GLWTVQGANLETIFRKDTEGLWEELQQQSRRIRAGSPTDQALALVR"
     gene            complement(1289..1765)
                     /locus_tag="VFR57_08725"
     CDS             complement(1289..1765)
                     /locus_tag="VFR57_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0259484.1"
                     /translation="MAKPGLTDANFSQTVVLASQAADGSTVGVILNRPTLAKHEETGA
                     TLFFGGPVMREVVVALFRSERAPDAAAFHVANGIYLTMHPQNLENLFGDRAARASGGY
                     RLFTGFAGWAPGQLQSELARGDWFVLPISAGVLFRDDTSGMWDELVRKARGRVAMR"
     gene            1943..2158
                     /locus_tag="VFR57_08730"
     CDS             1943..2158
                     /locus_tag="VFR57_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU0259485.1"
                     /translation="MAEEVRAPLAGNIWKVLVEAGAKVEEDDELVIIEALKMENAIYA
                     PRAGTVKEVCVKAGDKVAEDDLLVVIE"
     gene            2189..3349
                     /locus_tag="VFR57_08735"
     CDS             2189..3349
                     /locus_tag="VFR57_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0259486.1"
                     /translation="MNFELSEDQRLIQETARRFAAEQIAPTLEEEERRHEFRADRVAK
                     MGELGFFSCALPEKLGGSGMGFMESVLMAEQIAKVSASWRLPFNMQNLGPALTVAKFG
                     TEAQRKKYVPGWVAGTQLGFFAMTEPNTGSDVASMKTHALDMGDHWEVHGSKMWISQA
                     QVGDAGLLYAYTDRDKGNKGITAFIIEPKKMKGCTARGIETKLGLHCAPTGEFVFEGM
                     KVPKENVLGKPGEGFRMCMWQLNQTRLGCAAGALGVAGGALELAVNYANERKQFGRPI
                     AQHQMIQAQIADMVVEHQAAQVLVYRAAWLKDQAKPSQYETSVAKLAASEAAVHAANE
                     CMKIFGSYGYSTEFPAERFYRDAKSLQIVEGTSNIQKIIISGMASGATPNRE"
     gene            3355..5088
                     /locus_tag="VFR57_08740"
     CDS             3355..5088
                     /locus_tag="VFR57_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0259487.1"
                     /translation="MRPYFERMPQFGKPLKENRLARTQESRAKLEAVEAEIAAAAKAR
                     REVGLPAAEINKRGALTVWQRLEYLVDPGSWHPLHSLYNPMDNEEGTTNVVDGLGRIG
                     GRWAVIIGFDNKVLAGAWLPGQAENVLRVTDLANRLHIPLVWLVNCSGVKLPDQEKFY
                     ADRRGSGTCFFRHAELEQAGIPVLAGIYGTNPAGGGYQGISPTILIAHKDANIAVGGV
                     GIVSGMAPRGGFDVGMLEELIAKTRDFRERPPGRVETHYGETGFFARVYDEEKGVLDG
                     IKEYMRAIPAYEPMFFRVAAPAEPRLAAADLYHILPFNQKEVYSFDDLLARLVDASEH
                     MEYRPGYGPEVYTGLVKVDGMLLGAIGNRQGLLPKGYPEYAAYPGIGGKLYRQGLIKL
                     NEFVTHCGRDRVPVVWFQDTSGIDVGDLAEKAELLGLGQSLIYSIQQTEVPMMLVVLR
                     KGTAAAHYIMGGPTANRHNAFTLGTAATEIYVMHGETAAVATYARRAVKEKDSDRPLE
                     PIAEKMNEMARKYHDTSRPAYCARHGFVDEVVALQALRGYLKAFAGCVYQNPRSICPR
                     HLMMLPRLIKG"
     gene            complement(5092..>5621)
                     /locus_tag="VFR57_08745"
     CDS             complement(5092..>5621)
                     /locus_tag="VFR57_08745"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEU0259488.1"
                     /translation="PATKLLFPIVVTLVTGLIAPKGLPLMGAIMLGNFMRECGVVARL
                     TKASENEIANVVTLFLGLAIGGTMEGRAFLTPQTLAIFGLGFIAICLDTVAGVCFGKL
                     VCWMSGGKVNPLVGAAGISAYPMAARVVQQQGQRYDKRNYLLMHAAGANTGGQIGSVM
                     AAAVMLSVMKGLGMI"
BASE COUNT         1040 a         1816 c         1898 g          867 t
ORIGIN      
        1 agcgcatgtc gccgccggga acctccgatt gctccggctc ccagtacttg gcgttgccct
       61 tgaacgtgaa ggtcttgatg cccgcgagcc gctcgtagcc gcccatggct tccacggcgc
      121 gctcgacgag gctttgctct cgggttgtgg cgcaggaaac gagaagaacg gcgccggcga
      181 gcgccgcgac aacctgcttc atgacgggtc ctcccaggat ggatcgaaac cagcctaggc
      241 gaacacgggc tcgttttcaa ctattccggc ttcttcggtg cctgggtgcc gccggcggct
      301 gcgttgggag gagccttggc tgtgtcgacc aggccgaatt cgccgcgccc gatgcggcca
      361 tcgcggtcgc ggtcgacggc aatccagttg gcttgccttg cctcgtcgct ggcaagctcc
      421 gcagagctca agtagccatc ccggttgcgg tccaggcggt cgaatagcga acgcgcgggc
      481 gcgcttgcgc cgctcgtcgc actcggcccg gcgccggtcg gcgagccgcc tgggcagaga
      541 aggagcgccg cggcaagcgc cagccactcg aatccggaaa gccgcaccat gcgggccaga
      601 atagcccgcc ggcccgacga ctagttacgt cgccggggtg agccggtcag cgcacgagag
      661 cgagtgcctg gtcggtcgga gagccggcgc ggatgcggcg cgattgctgt tgcagctcct
      721 cccacaggcc ctcggtgtcc ttgcggaaga tggtctcgag attcgcgccc tgcaccgtcc
      781 acaggccgcg ttcgatctcg ctcttgagct cacccggacg ccagccgaca tagcccacga
      841 aatagcgcgc ctcgttcggc gtggtctcga tgacgtggtc gatcgtgttg gcgcggaacg
      901 cgaggtaaag gctcttcatc agcagcaccg agcccgtgcc cggcgcgttg tcggccttga
      961 ccagcgcgac gagcgcgccg cgcgagaagg gcccgccgta gtacacgggg tcggcgaccc
     1021 tcttggacgg ctcgtgctcg ggaaagaggc tgccgagcga gcgccgcgtc gggcggttga
     1081 ggatcacccc cacatggccg ccattcggcg cgggcgcagc gatcagcacc gtctgccggt
     1141 actcgaggtc gcgaaacgct ggatgcgcga ccaggatcac cgcctcatcc tgaacttggg
     1201 cgacggcgga actgcaggcg gcgaggatgc ctgtgacaag cgcgacgatg cgaagggcaa
     1261 cgctcatggg gccagtatac ttttcctttc agcgcatcgc cacacggcca cgggcttttc
     1321 tcacaagctc gtcccacatc ccgctcgtgt cgtcgcggaa gagcacgccg gcgcttatgg
     1381 gcagcacgaa ccagtccccg cgcgcaagct cgctctgaag ctgtccgggc gcccagccgg
     1441 cgaatcccgt gaaaaggcgg tagccgccgc ttgctcgcgc cgcacgatct ccgaacaaat
     1501 tttcaaggtt ctgcgggtgc atcgtgaggt atatcccgtt ggccacgtga aacgccgcgg
     1561 cgtcgggtgc gcgctccgac cgaaacagcg ccaccaccac ctcgcgcatc acggggccgc
     1621 cgaaaaacag cgtcgcgccc gtctcttcgt gcttcgccag cgtgggccgg ttgaggatca
     1681 cgccgaccgt gctgccgtcg gcagcctggc ttgccagcac cacggtctgg ctgaagttcg
     1741 cgtcggtgag accaggcttc gccaccagca gcacgccgtt cggcggctcg tgctgagttt
     1801 gcgcgcctag ggagaacgat atcccgaggg cgacccaggc gagccaccgc gccagtctct
     1861 gttcagtgct cagtcaaggc gattatgccg gcagagcctg tttttgatta agctcaagca
     1921 cgaacatcga aagggggaca ccatggccga ggaagtccgg gccccgctgg cagggaacat
     1981 ctggaaggtt ctggtcgagg cgggggcgaa ggtggaggaa gacgacgagc tggtgatcat
     2041 cgaggcgctg aagatggaga acgcgatcta cgcgccgcgc gccggaacgg tgaaggaagt
     2101 gtgcgtaaag gcgggcgaca aggtggcgga ggacgatctg ctggtcgtga tcgaatgaag
     2161 cggtccctag ggtgaaaggc acgaggcgat gaatttcgag ctgagcgaag accaacggct
     2221 gatccaggag acagcgcggc gctttgccgc cgagcagatc gcgccgacgc tcgaggaaga
     2281 ggagcgcagg cacgaattcc gtgccgaccg cgtggcgaag atgggcgagc tcgggttttt
     2341 ctcctgcgcg ctgcccgaga agctgggcgg cagcggcatg ggcttcatgg agtcggtgct
     2401 gatggccgag cagatcgcca aggtatcggc gtcgtggcgg ttgcccttca acatgcagaa
     2461 tctcggcccg gcgcttacgg tggcgaagtt cggcaccgag gcacagagga agaaatacgt
     2521 ccccggctgg gttgccggca cgcagctcgg atttttcgcc atgaccgagc cgaacaccgg
     2581 atccgacgtg gcgagcatga agactcatgc gctcgacatg ggcgaccact gggaagtgca
     2641 tggcagcaag atgtggattt cgcaggcgca agtcggcgac gccggcctgc tctatgccta
     2701 caccgaccgc gacaagggca acaagggcat caccgccttc atcatcgagc cgaagaagat
     2761 gaagggctgc accgcgcggg ggatcgagac caagctcggc ctgcattgcg cgccgaccgg
     2821 cgaattcgtg ttcgagggca tgaaagtgcc gaaagaaaac gtgcttggca agcccggcga
     2881 ggggtttcgc atgtgcatgt ggcagctcaa ccagacgcgc ctcggctgcg ccgccggcgc
     2941 gctcggcgtc gcgggcggcg cgctcgagct cgcggtgaat tacgccaacg aacgcaagca
     3001 gttcggaaga cccatcgcac agcatcagat gatccaggcg cagatcgccg acatggtggt
     3061 cgagcaccag gcggcgcagg tgctggtcta ccgcgcggcc tggctcaagg accaggccaa
     3121 gccgagccag tacgagacct cggtggcgaa gctcgccgcc tcggaggctg cggtgcacgc
     3181 ggcgaacgag tgcatgaaga tcttcggctc gtacggctat tccaccgaat ttcccgccga
     3241 gcgcttctac cgcgacgcga agtcgctgca gatcgtcgaa ggcacctcga acatccagaa
     3301 gatcatcatc tccggcatgg cgagcggcgc cacgccaaac cgggagtagg aacgatgcga
     3361 ccgtacttcg agcgcatgcc gcagttcggc aagccgctca aggaaaaccg cctcgcgcgc
     3421 acgcaggaga gccgcgcaaa gctcgaagcg gtggaagccg agatcgccgc ggcggcaaag
     3481 gcgcggcgcg aggtcgggct gcctgcggcc gagatcaaca agcgtggcgc gctaaccgtc
     3541 tggcagcgcc tcgagtacct cgtcgacccc ggaagctggc atccgctgca cagcctctac
     3601 aacccgatgg ataacgagga ggggaccacc aacgtggtcg acggcctggg ccgcatcggc
     3661 gggcgctggg cggtgatcat cggcttcgac aacaaggtgc tcgccggcgc ctggctcccc
     3721 gggcaggccg agaacgtgct gcgcgtgacg gacctcgcaa accggctcca tattccgctc
     3781 gtctggctgg tgaactgcag cggcgtgaag cttcccgacc aggaaaagtt ctatgccgac
     3841 cgacgcggct cgggcacctg cttcttccgt catgccgagc tcgaacaggc cggaatcccg
     3901 gtgcttgccg gcatctacgg caccaatccg gcgggcggcg gctatcaggg catcagcccg
     3961 acgatcctga tcgcgcacaa ggatgcgaac atcgcggtgg gcggggttgg catcgtctcc
     4021 ggcatggcgc cgcgcggcgg cttcgacgtc ggcatgctgg aagagctgat cgcgaagacg
     4081 cgcgacttca gggagcggcc gccggggcgc gtcgagacgc attacggcga aaccggattc
     4141 ttcgcccgcg tctacgacga ggagaagggc gttctcgacg gcatcaagga atacatgcgg
     4201 gcgatccccg cctacgagcc gatgttcttt cgcgtcgcgg cgccggccga gccgcggctc
     4261 gccgccgcag acctgtacca catcctgccg ttcaaccaga aggaggtgta ttcattcgac
     4321 gacctgctcg cgcgcctggt cgacgcgagc gagcacatgg agtaccggcc gggctacggc
     4381 ccagaggtct acaccggcct cgtcaaggtg gacggcatgc tgctcggcgc gatcggcaac
     4441 cgccaggggc tgctgcccaa aggctacccc gaatacgcag cctatcccgg gatcggcggc
     4501 aagctctacc ggcagggtct catcaagctg aatgagttcg tcacccattg cgggcgcgac
     4561 cgcgtgccgg tggtctggtt ccaggacacg agcggcatcg acgtcggtga cctcgccgaa
     4621 aaggcggagc ttctcggcct tggccagtcg ctcatctact ccatccagca gaccgaggtg
     4681 ccgatgatgc tcgtcgtgct gcgcaaaggc acggcggcgg cgcactacat catgggcggg
     4741 ccgacggcca accgccacaa cgcgttcacc ctcggcaccg ccgcgaccga gatctacgtc
     4801 atgcacggcg agaccgcggc agtggccaca tatgcgcggc gcgcggtcaa ggagaaagac
     4861 tctgaccggc cgctcgagcc gatcgcggag aaaatgaacg agatggcgcg caagtaccac
     4921 gacacctcgc ggcccgcgta ctgcgcgcgc cacggcttcg tcgacgaagt ggtcgcgttg
     4981 caggcactgc gcggctacct gaaggccttc gccggctgcg tctaccagaa cccgcgctcg
     5041 atctgcccgc ggcacctgat gatgctgccg cggctgatca agggctaggc atcagatcat
     5101 ccccaggccc ttcatcaccg agagcatcac ggcggcggcc atcaccgagc cgatctgccc
     5161 gccggtgttc gcgccggcgg cgtgcatgag gaggtagttc ctcttgtcgt agcgctgtcc
     5221 ctgctgctgc accacccgcg cggccatcgg gtaggccgag atgcctgcgg cgccgacgag
     5281 agggttgacc ttgccgccgc tcatccagca cacgagcttg ccgaagcaca cgcccgccac
     5341 ggtatcgagg cagatggcga tgaaccccag gccgaaaatc gcgagggtct gcggcgtcag
     5401 aaaagcgcgg ccttccatgg tgccgccgat ggcgaggccg aggaacagcg tgacgacgtt
     5461 ggcgatctcg ttctccgagg ccttggttag ccgagcgacg acgccgcatt cgcgcatgaa
     5521 attgcccagc atgatcgcgc ccatcagcgg cagccccttg ggcgcgatca gacccgtgac
     5581 gagggtcacg acgatgggaa agagaagctt cgtcgcggga g
//
LOCUS       DASTPD010000265         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1567295, whole genome shotgun sequence.
ACCESSION   DASTPD010000265 DASTPD010000000
VERSION     DASTPD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567295"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..645
                     /locus_tag="VFR57_08750"
     CDS             28..645
                     /locus_tag="VFR57_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259489.1"
                     /translation="MSKSKMVLGLVLTWLICVSPAAFANPWANGAAHAAAQACNPGNA
                     VFCGSLGGGGGAGGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGSAGGAGAGGAGGAGG
                     AGGGSAGTTSASASSAGGAGGSSAGTSQTGSGNSSGGSGSGTSGLGTFVFVAFAGLMI
                     YVMYMDDKCRRTPGCKPDPNLPTTNAVDSFGDKILTAWNTGPYLP"
     gene            complement(770..1264)
                     /locus_tag="VFR57_08755"
     CDS             complement(770..1264)
                     /locus_tag="VFR57_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259490.1"
                     /translation="MRTWRVSFLMGVCLIASTSVAAAPPAPKPLEGVTAGVEVCPQEW
                     EQLCPDGATFFGTLHGKVNGERTSGVFKVEVMHQSPLSLTGETPVLGGSFVVRTTRGE
                     VGGTITGGTLATDDGGETFKVLLTLLVTQPPSTTPLTFKGLLDHSGLKEEIPEPPTIT
                     GRIS"
     gene            complement(1603..2151)
                     /locus_tag="VFR57_08760"
     CDS             complement(1603..2151)
                     /locus_tag="VFR57_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259491.1"
                     /translation="MRRGAAALAVLALLAGCGGNATHAVPRTFDLGVTPPAVKFPALR
                     VASRAVAPFDGVHMHYRLAWRNPSELADYAHSFWAAPPAELLRKQLLRSTGEGPGKCT
                     LEIEVQEFTQVFSAKESSEARIEMRVALATGPSRIASRGVTVIEPNAGGEASSGATAM
                     ARAADRALDVLAAWVAAQPGCR"
     gene            complement(2148..3110)
                     /locus_tag="VFR57_08765"
     CDS             complement(2148..3110)
                     /locus_tag="VFR57_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0259492.1"
                     /translation="MESRAYAFITGLFVVALVAAIVVWANWLAQEPEDRKAYRVIATT
                     PVSGLNAQAQVRYRGMSVGRVTSIKLDPTDRHKILIDIEVNGDIPVTRGTYAQLGMEG
                     ITGIAYVHLLDDSQETAPPPQGASGLAQLPLRPSFLDTMTDSAEGTVKDARELIAALN
                     AVLTPENRNNINLMLASLQKTAANLETASAKLPSTVARADAWLGPENLRLARDSLEKV
                     NETANALPQIASDAERLVADLRTLAAEVEKLATQARELVAEIGQLSAEIKGDTLPQVG
                     AFAESADRSARKIGNLAGDLSRNPDSVLWGREAGRPGPGEPGFQ"
     gene            complement(3116..>3923)
                     /locus_tag="VFR57_08770"
     CDS             complement(3116..>3923)
                     /locus_tag="VFR57_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259493.1"
                     /translation="RSSCAMSADPTEYAIEVEGLVNAVGAGQILHQNLNLKVRRDEVM
                     TLVGGSGSGKTQLLRVILGLKHPLAGSVRVFDVSWYDPSFKKVRDARRRMGMLFQNGA
                     LFSALTVFDNIAFPLRERGGVSEKKIRAIVHAKLAEVELAPHHGTLLPANLSGGMVKR
                     VALARALALAPDLLILDEPTAGLDPDRSGRFVRLIKRLRADHRFAVFMVTHDRDTLYA
                     LTDRVAVLGDRHIIACGTQEEVRELPHPFVQSFFGGERGRLALAPDEAVA"
BASE COUNT          654 a         1266 c         1281 g          722 t
ORIGIN      
        1 cccgagtgaa gaagggagtt ttctgagatg tcgaaaagca agatggttct gggcctcgtt
       61 ctcacttggc tcatttgcgt cagcccggcg gcgtttgcca atccgtgggc gaacggcgcg
      121 gcgcacgccg cggcgcaggc ctgcaatcct ggcaacgcag tgttctgcgg ttcgctcggc
      181 ggtggcggtg gagcgggcgg tggagcaggt gcgggcggcg caggcggcgc aggtgcgggt
      241 ggcgcaggcg gcgcgggcgc gggtggcgca ggcggcgcgg gcgcgggtag cgcaggcggc
      301 gcgggtgcgg gtggcgcagg cggcgcgggt ggagcgggtg gtggttcggc cggcacgact
      361 agcgcgagcg cgagcagcgc gggtggtgcg ggtggtagtt cggccggcac ctcgcagacc
      421 ggcagcggca actcctcggg tggctcgggg tcgggtacca gcgggctcgg caccttcgtg
      481 ttcgtcgcct tcgccgggct gatgatttac gtcatgtaca tggacgacaa atgccgtcgt
      541 actccgggct gcaagccgga cccgaacctg ccgacaacca acgcggtcga cagcttcggc
      601 gacaagatcc tcacggcctg gaacaccgga ccgtatttgc cgtaggcacc aaagcaagcc
      661 aagtagttga attaggaaag cagcgaagcg gctgctctcc cgtctggacg gcctgatcgc
      721 gcaaccacgg gtctactacc ctcgctcagc ttccatcgct atttcggtac taagaaattc
      781 tccccgtgat ggttgggggt tcaggtattt cctcttttag tccgctatgg tctaggaggc
      841 ctttaaacgt taacggcgta gtgctgggtg gctgagtaac tagaagtgtc aagagaacct
      901 tgaacgtctc gccgccgtcg tcggtggcca gtgtgccgcc ggtgatcgtc ccgccaacct
      961 ctccccttgt ggttcgaacg acaaaggagc caccaagcac cggggtctcg ccggtgagac
     1021 ttagaggaga ctgatgcatg acctcgactt tgaacacacc gctggtgcgc tcgccattga
     1081 ctttcccgtg caaggtaccg aagaaggtcg cgccatctgg acacaactgc tcccactcct
     1141 gcggacaaac ttccacgccg gcggttaccc cctcgagcgg cttgggagcg ggaggagcgg
     1201 cggccaccga cgttgatgcg attaagcaca cccccattaa aaagcttaca cgccaggtcc
     1261 gcatatgcac ctccttacag ttagttccgt tcgcacaaat gcccgcgtcg ccatgcttag
     1321 cgctcggaaa cgaacgcaat cggcgctacc tgtttagggt tcctagcatt tgatgtgccc
     1381 tgcaaggcag tcgtcccgag caatcgcgcg cgaatcacct cgtagttcgc ctaaatccag
     1441 gctcggcatc ccggcaatgc ggagcaatga gctgttgccc gctaagcgct ttctacgcgt
     1501 cactcttgca cgacatctta tttcgattcg aatacttgaa ggcgcccgaa gcgaacctga
     1561 tgggctgatc cagagtagta tttgtgggga gcctctcaga ccttatctgc accctggctg
     1621 cgctgcgacc caggcagcga gcacgtcgag cgcgcgatcg gccgcacgcg ccatcgccgt
     1681 tgcacccgac gaagcctcgc cccctgcatt gggctcgatc acggtcacgc cgcgcgaggc
     1741 gatgcgtgac gggccggtcg cgagggcaac ccgcatttca atgcgcgcct cgctggattc
     1801 cttggcagag aaaacctgcg tgaactcctg aacctcgatt tccagcgtgc acttgccggg
     1861 gccttcaccg gtcgagcgca ggagttgctt ccgcagcagt tcggccggcg gggctgccca
     1921 gaagctgtgc gcatagtcgg cgagctcgga cggatttctc cacgccagcc ggtagtgcat
     1981 gtgcacgccg tcgaacgggg caaccgcgcg cgacgcgacg cgcaacgcgg gaaatttcac
     2041 ggctggcggc gtaactccga gatcgaacgt acgcggcacc gcgtgcgttg cattgccgcc
     2101 gcagccagcg agcagcgcga gaaccgcgag cgccgccgcg cctctcctca ttggaacccg
     2161 ggctcgccgg gccccgggcg tcctgcctct cttccccaaa gcacgctgtc cgggtttcgc
     2221 gacaggtcgc cagcgagatt gccgatcttg cgcgcgctcc gatccgccga ttcggcgaac
     2281 gcgccgacct gcggcagcgt atcgcccttg atctcggccg aaagctggcc gatttcggcg
     2341 acgagctcgc gcgcctgcgt tgccagcttt tcgacttcgg cggcgagcgt gcgcagatcg
     2401 gcaaccagcc gctcggcatc gcttgcgatc tgcggcagcg cgttcgccgt ttcgttcacc
     2461 ttctcgagcg agtcgcgcgc gaggcgcagg ttctccggtc cgagccaggc gtccgctcgc
     2521 gcgacggtgc tcgggagctt cgcgctcgcg gtttcgaggt tcgccgcggt cttttgcagc
     2581 gacgcgagca tcaggttgat gttgttgcgg ttctccggag tcaacactgc gttgagcgcc
     2641 gcgatcagtt cgcgcgcatc ctttacggtg ccttctgcgc tgtcggtcat cgtgtcgagg
     2701 aacgaaggcc tgagaggaag ctgcgcgagc ccgcttgccc cctgcggcgg cggagcggtc
     2761 tcctggctat cgtcaagcag gtgcacatag gcgatcccgg tgatgccttc catgccgagc
     2821 tgcgcgtacg ttccgcgcgt caccgggatg tcgccgttca cctcgatatc gatcaggatc
     2881 ttgtggcgat ccgttggatc gagcttgatc gaagtgacgc gaccgacgct catgccgcgg
     2941 taacgcacct gcgcctgcgc attgagccca ctgaccggcg tcgtcgcgat cacgcgatac
     3001 gcctttcgat cctccggctc ctgcgccagc cagttcgccc agacgacgat cgccgcgacg
     3061 agcgcgacga cgaagagccc cgtgatgaat gcgtatgcgc ggctttccat tggtcttatg
     3121 cgacggcctc gtctggcgcg agcgcaaggc ggccgcgttc tcctccgaag aagctctgca
     3181 cgaacggatg cggcagttcc cgcacttcct cctgcgtgcc gcaggcaatt atatgccggt
     3241 cgccgagcac ggcgacgcgg tcggtgagcg catagagcgt gtcgcggtcg tgcgtcacca
     3301 tgaacaccgc aaagcggtgg tcggcgcgca gccgcttgat cagccgcacg aagcgcccgg
     3361 agcggtcggg atcgagcccc gcggtcggct cgtcgaggat cagcaggtcg ggcgcgagag
     3421 caagcgcgcg cgcgagcgcg acgcgcttca ccattccgcc cgaaaggttc gccggcagca
     3481 gcgtgccgtg gtgcggcgcg agctcgactt ccgcgagctt cgcatgcacg atcgctcgga
     3541 tcttcttctc cgaaacgccg ccgcgctcgc gcagcggaaa ggcgatgttg tcgaacacgg
     3601 tcagcgcgga aaaaagtgct ccgttctgga acagcattcc catgcggcgc cgcgcgtcgc
     3661 gcactttctt gaagctaggg tcgtaccacg acacgtcgaa tacgcgcacc gagcccgcga
     3721 gcgggtgctt caatccgagg atcaccctca gcagctgcgt cttgccgctg cccgacccgc
     3781 cgacgagcgt catcacctcg tcgcgccgca ccttgaggtt caggttctgg tgcaggatct
     3841 ggcccgcgcc gaccgcgttg acgaggcctt cgacctcgat tgcgtattcg gtggggtcag
     3901 ccgacattgc gcaagaggat cgc
//
LOCUS       DASTPD010000266         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1364068, whole genome shotgun sequence.
ACCESSION   DASTPD010000266 DASTPD010000000
VERSION     DASTPD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364068"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFR57_08775"
     CDS             <1..817
                     /locus_tag="VFR57_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259494.1"
                     /translation="MSEMRVTRQVLSPMPELLSYWLPLDDAKVLIRHLNEQIAEMLRA
                     APERFVGLGCVPLQDTDSAIRELEFLKRDLNFPGVEIASHVNGVSIGDARFEPFFAAA
                     ERLGAAIFVHALRPVGQERIVGAFPEQAVCFPGDVALACASMITGGIASRHPKLRIAF
                     SHGGGTMSILMPRLVHAWNNFPKAKESLQESPATIARRFYYDELVFEPNAVRFLVETF
                     GESQIVVGTDYPFALGDWKPLETLKKSGLEERVLTGITSANARRFLGLPEGSR"
     gene            complement(765..1532)
                     /locus_tag="VFR57_08780"
     CDS             complement(765..1532)
                     /locus_tag="VFR57_08780"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEU0259495.1"
                     /translation="MELVRSARSWVDAAKRVVVLTGAGISTDSGIQDFRGPQGLWTKN
                     PLAEKLSNIHYYLADREVRKAAWKNRMASPAWTAKPNPGHLALVHLEKRRKLHALITQ
                     NIDELHQQAGNSPDRVIEVHGSMRRFICWGCGMRGPMQLVLERVRAGEEDPECRTCGG
                     ILKSDTISFGQQLVPEVIDRALQVAGEADVFVAVGTTLQVYPVAGAVDIARSAGAKVI
                     IVNAEPTPYDDVADAVLPGSISEILPAILGSGARLQK"
     gene            complement(1536..1931)
                     /locus_tag="VFR57_08785"
     CDS             complement(1536..1931)
                     /locus_tag="VFR57_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259496.1"
                     /translation="MATKPETTNEIKVDPKALYLEEIFTDRRVGTIRRLTPVNKDGAR
                     DPARAVLYVGETQVLTPAGALPIAFEIGAGSLEEAAEKFGQLAKEAIERTVKELQELR
                     RQAASSIVIPQGGMPPMPGVGGGGKIQMP"
     gene            complement(1941..2762)
                     /locus_tag="VFR57_08790"
     CDS             complement(1941..2762)
                     /locus_tag="VFR57_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0259497.1"
                     /translation="MRQVSRIERVIPQGPSSQVEAKQLVIGAEGWEKTSPFLFLSEDW
                     FGRPKGFETHPHRGMQTVTIVLEGALEHRDHTGAHGRLGAGDVQWMTAGHGVLHSEMP
                     CGDETAHTLQLWLNLPAKLKMIPARYQDQRLENTPVRIVPGGKVVVYAGRSGEVSHPH
                     GSDYPMSLLDIRLGAGARLAQEVSTQDRGFVYVLGGRAAIGEKDVKKGEVAWFEPGEG
                     SELVLEAKEDVRGVLFAGEPIYEPAVAYGPFVMNTRQEIEQAFRDYQAGTLVGQR"
     gene            complement(2759..>3617)
                     /locus_tag="VFR57_08795"
     CDS             complement(2759..>3617)
                     /locus_tag="VFR57_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU0259498.1"
                     /translation="TFPLAVGATTVLMAERPTPDSVFARLVDEKPTIFYGVPTLYAAL
                     LASPNFPKKDQLAMRMCTSAGEALPPEIGKRWTERTGAELLDGIGSTEMLHIFLSNRP
                     GDVRPGTTGKPVPGYQLRLVDDQGNVVTKPGELGELQIGGPTSAIMYWNQREKTKNTF
                     QGPWTRSGDKYSLDKDGYYVYGGRSDDMLKVSGIYVSPAEVEAALVSHEAVLEAAVVG
                     AEDESKLVKPKAFVVLKQGNTPNEELKHRLQQHVKDKLAPYKYPRWIEFMSELPKTAT
                     GKIQRFKLR"
BASE COUNT          567 a         1210 c         1148 g          692 t
ORIGIN      
        1 gatgtcggaa atgcgcgtga cccggcaggt gctctcgccg atgccggagc tcctgtcgta
       61 ctggctgccg ctcgacgacg cgaaagtgct gatccgccac ctcaacgagc agatcgcgga
      121 gatgctccgg gccgcgcctg agcgcttcgt cgggcttggg tgcgtgcctc tccaggacac
      181 ggacagcgcg atcagggagc tggaatttct gaagagggat ttgaatttcc ccggtgtaga
      241 gatcgcgagc catgtcaacg gggtctcgat cggcgacgcg cgcttcgagc ctttcttcgc
      301 cgcggccgag cggctcggcg ccgcgatctt cgtccacgcg ctgcgccccg tgggccagga
      361 acgcatcgtt ggcgcattcc ccgagcaggc ggtgtgcttc cccggcgatg tcgccctcgc
      421 ctgcgcctcg atgatcaccg gcggcatcgc ctcgcgccat ccgaagctgc gcatcgcctt
      481 cagccacggc ggcggcacga tgtcgattct gatgccgcgc ctggtgcacg cctggaacaa
      541 ttttccgaag gccaaggagt cgctgcagga atcccccgcg acgatcgcga ggcgcttcta
      601 ctacgacgag ctcgtcttcg agccgaacgc ggttcgcttc ctggtagaga ccttcggcga
      661 gagccaaatt gtcgtcggta ccgactatcc cttcgccctc ggcgattgga agccgctcga
      721 aaccttgaaa aaatcgggcc tggaggaacg agtccttacc ggaatcactt ctgcaaacgc
      781 gcgccgcttc ctaggattgc cggaaggatc tcgctgatcg agccgggaag cacggcgtcg
      841 gcgacatcgt cgtagggcgt gggctccgcg ttgacgatga ttaccttcgc gcccgcggag
      901 cgcgcgatgt ccaccgcgcc cgccaccggg tagacctgta gcgttgtgcc gacggcgacg
      961 aatacgtcgg cctcccctgc cacctgcaac gcccggtcga tcacctcggg cacgagctgc
     1021 tggccgaagg aaatcgtgtc gctcttcagg atcccgccgc aggtgcggca ctccggatcc
     1081 tcctctcccg cgcgcacgcg ctcgagcacg agctgcatcg gcccgcgcat gccgcagccc
     1141 cagcaaatga agcggcgcat cgagccgtgc acctcgatca ctctgtcggg agagttcccg
     1201 gcctgctggt gcagctcgtc gatgttctgc gtgatgagcg cgtggagctt tcgtcttttc
     1261 tccagatgca ccagggcgag atgcccgggg ttgggcttcg cggtccaggc cgggctcgcc
     1321 atgcggttct tccacgcggc cttgcgcacc tcgcggtcgg cgaggtagta gtggatgttg
     1381 gagagcttct cggcgagcgg atttttcgtc cacagcccct gcgggccgcg gaagtcctga
     1441 attcccgaat cggtggaaat ccccgcgccg gtgagaacga cgacgcgctt ggctgcgtca
     1501 acccaagagc gcgctgagcg gacgagttcc aacccttagg gcatctggat cttgccgccg
     1561 ccgcctaccc cgggcatcgg cggcatgccg ccctgcggga tgacgatcga cgaggcggcc
     1621 tggcggcgca actcctggag ctccttcacg gtgcgctcga tagcttcctt ggcgagctgg
     1681 ccgaacttct ccgccgcctc ttcgagcgac ccggcgccga tctcgaaggc gatcggcagg
     1741 gcacccgcgg gcgtcagcac ctgggtctcg ccgacgtaga gcacggcgcg cgccggatcg
     1801 cgcgcgccgt ccttgttcac gggcgtaagc cgacggatcg ttccgacgcg ccgatcggtg
     1861 aagatctcct ccaggtacag cgccttcgga tcgaccttga tctcgttcgt ggtctcgggt
     1921 ttggtagcca tggcgcgatt ttaccgctgc ccgacgagtg ttcccgcctg gtagtcccgg
     1981 aaggcctgct caatttcctg tcttgtattc atcacaaacg gtccgtaggc cacggccggc
     2041 tcgtagatcg gctcgccggc aaagagcacg ccgcgcacgt cttctttggc ttccagcacc
     2101 aattcactgc cttcgccagg ctcgaaccac gcaacctcgc ctttcttcac gtccttttcc
     2161 ccgatggccg cgcggccgcc aagcacgtag acgaaaccgc gatcctgcgt agacacctcc
     2221 tgggcaagcc gtgcgccggc gccgagccgg atatcgagga gcgacatcgg atagtcgctt
     2281 ccgtgcgggt gggacacctc gcccgaccgg cccgcataga caacgacctt tccaccgggg
     2341 acgattcgca caggggtgtt ctcgagcctt tggtcctggt agcgagccgg gatcatcttg
     2401 agcttcgccg gcaggttgag ccagagctgc agcgtgtgcg cggtctcatc gccgcacggc
     2461 atctcgctgt ggagcacgcc gtgccctgcc gtcatccact gaacgtcgcc cgcccccagg
     2521 cggccgtgcg caccggtatg gtcccgatgc tcgagcgcgc cctcgagaac gatagtgacg
     2581 gtctgcatgc cgcgatgcgg atgcgtctcg aagcccttcg gtcgtccgaa ccagtcctcg
     2641 ctcaggaaaa ggaagggcga ggttttctcc cagccctcgg cgccgatgac gagttgcttc
     2701 gcttcgacct gcgagctcgg gccttgcggg ataacgcgct cgatgcggct gacctgcctc
     2761 aacgcagctt gaacctctgg atctttccgg tcgcggtctt gggaagctcg ctcatgaact
     2821 cgatccagcg cggatacttg tagggcgcga gcttgtcctt gacgtgctgc tgcaggcggt
     2881 gcttcagctc ctcgttcggc gtgttgccct gcttgagaac cacgaaagct ttcggcttca
     2941 ccagcttgct ttcatcttcg gctcccacca cggcagcttc gagcaccgct tcgtgcgaga
     3001 cgagagccgc ttccacctcg gccggagaca cgtagatgcc gctcaccttc agcatgtcgt
     3061 ccgagcgccc gccgtagacg taatagccgt ccttgtcgag gctgtacttg tcgccgcttc
     3121 gcgtccatgg accctggaag gtgttcttgg tcttctcgcg ctggttccag tacatgatgg
     3181 ccgaggtcgg gccaccgatt tgcagctcgc cgagctctcc cggcttagtg accacgttgc
     3241 cctggtcgtc caccagccga agctggtagc ccggcacggg cttgcccgtc gtccccggcc
     3301 gcacgtcgcc gggccggttg gagaggaaga tgtgcagcat ctcggtcgag ccgataccgt
     3361 cgaggagctc tgcgcccgtg cgctcggtcc agcgcttgcc gatctcgggg ggaagcgctt
     3421 cacccgcgga agtgcacatg cgcattgcga gttggtcttt cttcgggaag ttcggcgaag
     3481 cgaggagtgc cgcatacagg gtcggcacgc cgtagaaaat ggtcggcttc tcatcaacca
     3541 ggcgcgcgaa aaccgagtcg ggcgtcgggc gctcggccat caggaccgtg gtggcgccga
     3601 ccgcgagcgg gaaggta
//
LOCUS       DASTPD010000267         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1364500, whole genome shotgun sequence.
ACCESSION   DASTPD010000267 DASTPD010000000
VERSION     DASTPD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364500"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFR57_08800"
     CDS             <1..253
                     /locus_tag="VFR57_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0259499.1"
                     /translation="LARALGPHGIRVNAIAPGAIPGGGFQAPPGMVEKIPLGRFGTPA
                     DVAGMAVAVLAERFGAYVTGASIVVDGGIALHNWIDPAR"
     gene            266..424
                     /locus_tag="VFR57_08805"
     CDS             266..424
                     /locus_tag="VFR57_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU0259500.1"
                     /translation="MIWSIVLIVLGLLFLAHNLDFIQWKQLRDLLSIWWPLILIAVGL
                     AQLGRRRK"
     gene            421..1560
                     /locus_tag="VFR57_08810"
     CDS             421..1560
                     /locus_tag="VFR57_08810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0259501.1"
                     /translation="MNFELSEEQQRIREAIGKLCERFPDEYWLKRDLEGGFPADFHQA
                     FARDGWLGIAMPEAYGGGGLGITEAAIMMQAIAESGAGFSGASAVHMNIFGLNPVVVF
                     GTEEQKKRMLPPLIAGKERACFAVTEPNTGLNTQKIKVKAERRESTYVVSGQKVWIST
                     AQVAEKVLLLARAQAGLTLFYTDLDRKYVEVREIEKMGRHAVDSNQVFFDALPVPEAD
                     RIGEEGKGFEYILHGMNPERILIAAELVGLGRCAVKRAADYAKERVVFDRPIGQNQSI
                     QHPLAANWMALEAANLMAFKGASLYDAAKPCGAEANAAKYLAGEACFDACQQAVMTFG
                     GFGYAREYHVERYLRESFVGRIAPVSRELILCFIAERVLGLPKSY"
     gene            1690..1827
                     /locus_tag="VFR57_08815"
     CDS             1690..1827
                     /locus_tag="VFR57_08815"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEU0259502.1"
                     /translation="MKFLGWRKSAPDPASVPAIRRILHLELIGVAIILLAAALMAKGI
                     G"
     gene            complement(1824..2330)
                     /locus_tag="VFR57_08820"
     CDS             complement(1824..2330)
                     /locus_tag="VFR57_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0259503.1"
                     /translation="MERIEKSVEVACPVRTVYNQWTQFEDFPRFMAGVKEVKQLDDTH
                     VHWHAEIWGKDKEWDAEITEQVPDQVIAWRSTSGAPNAGTVRFEPLSHDRTRVRLIME
                     YEPEGVVEKTGDAIGVFSARVENTVEDFKKFIESRGAETGAWRGEVHGGQQTAQGSTR
                     GGGGRTIR"
     gene            2472..2861
                     /locus_tag="VFR57_08825"
     CDS             2472..2861
                     /locus_tag="VFR57_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259504.1"
                     /translation="MILKALTAATAAALLALSGATLAQTSGSSSTGASGTTGSSGASG
                     SSATGASSTDVPAKCVSLTGTEREKCLQEERSGAGSGTTGSGGLSGTSPSTSPSTSPS
                     PSPSSPSTSGSGGMSGSGGTTGSGTTK"
     gene            2868..3089
                     /locus_tag="VFR57_08830"
     CDS             2868..3089
                     /locus_tag="VFR57_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259505.1"
                     /translation="MLRKQLRFGRGFRVIPGNRSSQAAEMVLAPAAEGEKTEKHAIRN
                     TGRGLLRTLNFYSPPAYRKDGKPLPRGES"
     gene            complement(3109..3858)
                     /locus_tag="VFR57_08835"
     CDS             complement(3109..3858)
                     /locus_tag="VFR57_08835"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259506.1"
                     /translation="MTDSLGHRAKFAVLAPSTNTSVQPEFDAMRPWGVTNHHSRLVIP
                     DLRVTGDRSFMAMMDAIRGALFPALKAALTCDPDYVVLGMSAETFWDGREGSRRLKRK
                     LLSTAKRGVAMGSDACRAALRAYGRGVRRLGVITPYMPVGDQQVRRFFTESGYQVVNL
                     VGLKCGSPTLIAHVSKKELRDAIVRVNRGNVDAVVQVGTNLAMGEVAAAAEFWLDKPV
                     IAINTATYWYALRDYGIRDRIPGWGSLLTEH"
     gene            3891..4181
                     /locus_tag="VFR57_08840"
     CDS             3891..4181
                     /locus_tag="VFR57_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259507.1"
                     /translation="MTREEYVAKLKSQIDNWNAEAAKWEAKAKEAQVGMQAEYERQLA
                     QFRTRRDTAMNEVQRLQNASADAWKAMMQGADAALKSMQAAFEKARSEFQKK"
BASE COUNT          734 a         1423 c         1351 g          728 t
ORIGIN      
        1 gctcgcgcgc gcgctcgggc cgcacggcat ccgcgtaaac gcgattgcgc cgggcgcgat
       61 cccgggcggc ggcttccagg cgccgcccgg aatggtcgaa aagattccgc tcggacgctt
      121 cggcacgccg gccgacgtcg cgggcatggc cgtcgctgtt ctcgccgagc gcttcggcgc
      181 ctacgtcacc ggcgcgtcca ttgtcgtcga cggcggcatc gcgcttcaca actggattga
      241 ccctgcgcgc tgaggccgcg acgctgtgat ctggtcgatc gtgctcatcg tgctcggcct
      301 gctcttcctc gcgcacaacc tcgatttcat ccagtggaag caactgcgcg acctgctatc
      361 gatctggtgg ccgctcattc tcatcgcggt cggcctcgcg cagctcggcc gacgcagaaa
      421 atgaacttcg agctgagcga agagcaacag cggattcgcg aggcaatcgg caagctctgc
      481 gagcgctttc ccgacgagta ctggctcaag cgcgacctcg agggcggctt cccggcggat
      541 ttccaccagg cgttcgcgcg cgacggctgg ctcggcatcg cgatgccgga agcctatggc
      601 ggcggggggc tcggtattac cgaggctgcc atcatgatgc aggcgatcgc cgagtcgggt
      661 gcaggattct caggcgcttc cgccgttcac atgaacattt tcggcctgaa ccccgtcgtg
      721 gtattcggca ccgaggagca gaaaaagcgc atgctgcccc cgcttatcgc cgggaaggag
      781 cgcgcctgct ttgcggtgac cgagccgaat acgggtctca atacccagaa aatcaaggtc
      841 aaagctgaaa ggcgcgaaag cacgtacgtc gtctccgggc agaaggtctg gatctccacc
      901 gcgcaggtcg ccgaaaaagt gttgctgctc gcgcgggccc aagccgggct tacgcttttc
      961 tatacggacc tcgaccgcaa atacgtcgag gtgcgcgaaa tcgagaagat gggccggcac
     1021 gcggtcgact cgaaccaggt gttcttcgac gcgctgccgg tccccgaggc cgaccgcatc
     1081 ggcgaggagg gcaaaggctt cgagtacatc ctccacggca tgaaccccga gcgcatcctg
     1141 atcgcggccg agctcgtcgg actcgggcgc tgcgcggtga agcgcgcggc ggactacgcg
     1201 aaggagcgag tggtgttcga ccggccgatc ggccagaacc agtcgatcca gcacccgctc
     1261 gccgcgaact ggatggcgct cgaggcggcc aacctgatgg cgttcaaggg ggcgtcgctt
     1321 tacgacgcgg ccaagccttg cggcgccgag gcgaacgccg ccaagtacct cgcgggcgag
     1381 gcctgcttcg acgcctgcca gcaggcggtg atgaccttcg gcggattcgg ctacgcgcgc
     1441 gagtaccacg tcgagcgcta cctgcgcgag tccttcgtcg gccgcatcgc gccggtgagc
     1501 cgcgagctta tcctctgctt catcgccgag cgcgtactcg gcctgccgaa gtcttattga
     1561 tgagcgcctt catggcgttt ctgcaccacc tggccgcgtt cgcggtagtg gtggcgatcg
     1621 cagtcgagtt cgtatttatt cgcgacgacc cttttcgtcc tgattgcgct cgcctcgatc
     1681 tacccgacgc tgaagttcct cggctggcgc aagagcgcgc cggacccggc gagcgtgccg
     1741 gccatccggc gcatcctgca cctcgagctc atcggcgtgg cgatcatcct gctcgccgcc
     1801 gcactgatgg cgaaaggcat cggctagcgg atcgtacggc caccgccgcc acgcgtactg
     1861 ccctgcgcgg tttgctgccc gccgtgcact tcaccgcgcc atgcgccggt ttcggcgccg
     1921 cggctctcaa tgaacttctt gaagtcctcg acggtattct ccacacgcgc gctgaacacg
     1981 ccgatcgcgt cgccggtctt ctccaccacg ccttctggct cgtactccat gatcagccgc
     2041 acgcgcgtgc ggtcgtgcga tagcggctca aacctcacgg tgccggcgtt cggtgctccc
     2101 gaggtgctgc gccacgcaat cacctggtcg ggcacctgct cggtgatctc cgcatcccac
     2161 tccttgtcct tgccccagat ctccgcgtgc cagtgcacat gggtatcgtc aagctgcttc
     2221 acttccttca cgccagccat gaagcgcggg aagtcctcga actgcgtcca ctggttgtaa
     2281 accgtacgca ccgggcaggc gacttccacc gacttctcga ttctttccat gtctattcct
     2341 ctcgctggat tgacgtttgc ggcgagtgcc gcaagaagcg ttcccttcgg cttcggcagg
     2401 ctagggctgg gcatggtctt tgcatgccgt ctgcggcagt ccccaaccat actctcgagg
     2461 agacgcacgt catgattctg aaagcactta ccgccgcgac cgcagccgcg cttctcgcgc
     2521 tatcgggcgc gacccttgcc cagacgtcgg gctctagcag cactggcgcg agcggtacga
     2581 ccggcagctc gggcgccagc ggcagctcgg ccaccggcgc ttcttccacc gacgttccag
     2641 ccaagtgcgt cagcctgacc ggcaccgagc gcgagaagtg tctgcaagag gagcgcagcg
     2701 gcgccggctc gggcacaacg ggcagcggcg gtctttccgg caccagcccg tccacttcac
     2761 cgtcaaccag cccgtcgccg tcgccgtcca gcccgtccac ttcgggtagc ggcggcatgt
     2821 ccggcagcgg tggcacgacc ggcagcggca ccaccaagta gaggcagttg ctgcggaagc
     2881 agttgcggtt cggccgggga ttccgcgtaa tccccggcaa ccgctcctcg caggccgccg
     2941 aaatggtgct cgctcccgca gccgagggcg agaagacgga gaagcacgcg atccgcaata
     3001 ccgggcgagg cttgctgcgc acgctcaact tctattcgcc gcccgcctat cgcaaagacg
     3061 gcaagccgct tccgcgcggg gaaagctagg gtcgcgttcg ggagccgctc agtgctccgt
     3121 caggagcgat ccccagccgg ggatgcggtc gcggatgccg tagtcgcgca gtgcgtacca
     3181 ataggtagcc gtgttgatcg cgatcacggg cttgtcgagc cagaactccg cggccgcggc
     3241 gacctcgccc atcgccagat tcgtgccgac ctggactaca gcgtcgacat ttccacggtt
     3301 cacgcgcacg atcgcgtcgc gcagttcttt tttcgacaca tgcgcgatca gcgtcgggct
     3361 cccgcatttc aggccgacca ggttcaccac ctggtagccg gattcggtga agaaacggcg
     3421 cacctgttga tccccgaccg gcatgtacgg agtgatcacg ccgagacgcc tgacgccccg
     3481 gccgtaggcg cgcagcgccg cgcggcaggc gtccgacccc atcgctaccc cgcgcttcgc
     3541 ggtcgatagc aactttcgct tcagtcttct ggagccttcc cgcccgtccc agaatgtctc
     3601 ggccgacatg ccgagcacca cgtagtccgg gtcgcatgtg agcgcggcct tgagcgcggg
     3661 gaaaagcgcg ccgcggatcg cgtccatcat cgccatgaac gaacgatcac ccgttacgcg
     3721 cagatccggg atcacgaggc gcgaatgatg gttggtcacg ccccacggac gcatggcgtc
     3781 gaactccggc tgcaccgagg tattggtgga cggcgcaagc actgcgaact tggcacgatg
     3841 gccgagcgaa tcggtcacgg cgttagaatg ccacagcaag gaggcagacc atgacccgag
     3901 aggagtacgt cgcgaagctc aagtcgcaga tcgacaactg gaacgccgag gccgccaagt
     3961 gggaagcgaa ggcgaaagaa gcgcaggtcg gcatgcaggc cgagtacgag cgtcagcttg
     4021 cacagtttcg cacccggcgc gatacggcga tgaacgaggt gcagcggctg cagaacgcct
     4081 ccgccgatgc ctggaaggcc atgatgcagg gcgccgacgc ggcattgaag agcatgcaag
     4141 cggcgttcga gaaggcgagg agcgaattcc agaagaaata agaaccgtcg ggcgcggcct
     4201 tgcgttcgag gacctgaggc tcggcgcgcg ctttcg
//
LOCUS       DASTPD010000268         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_548742, whole genome shotgun sequence.
ACCESSION   DASTPD010000268 DASTPD010000000
VERSION     DASTPD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548742"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFR57_08845"
     CDS             <1..222
                     /locus_tag="VFR57_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEU0259508.1"
                     /translation="DEAWDREFLELIERDPERLASMTVEDFMQRGGNEGAEVIMWLIM
                     RGALDERVRKLHQNYYLPMTTAMAVALFE"
     gene            215..535
                     /locus_tag="VFR57_08850"
     CDS             215..535
                     /locus_tag="VFR57_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEU0259509.1"
                     /translation="MSKYVFDLERSARGLRLNRFLNGLTKAENRRLFLEKPEDAMKVL
                     TEEERGMIRRLDWKALQDCGASFFCLEKLARTKGVSNPEMVAAFRGETLEQFLKTRRV
                     PGAR"
     gene            541..1050
                     /locus_tag="VFR57_08855"
     CDS             541..1050
                     /locus_tag="VFR57_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0259510.1"
                     /translation="MGPAWHERIAKVLKANEVRLVTYIPDNVLAPLISAVHADPWFKA
                     ICPAREEEAAGIVAGAHMAGLRGIVLMQTSGFATLPNVLASLIVPYRIPLLMLISERG
                     RLGDHQLGQAIVCSTMRPVLQTLGIEHFAIERLEDVEFVADGMIRQAYSTQAPAAVVL
                     SPLLTKKGA"
     gene            1047..1613
                     /locus_tag="VFR57_08860"
     CDS             1047..1613
                     /locus_tag="VFR57_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0259511.1"
                     /translation="MNRFDLTKRLVAKLKANEAVIGGIGNTNFDLWAAGQRPENFYML
                     GSMGLAVPIGLGVALAQPARRTFVLEGDGSLLMQLGSLGTVAASAPANLTIVVFDNGM
                     YHITGKQKTLTAGTVDLVAVARAAGLERSEWARDESHFESLVDRALRESGPAFIGARI
                     DGASPAGVTERDPARIRQRFMDALCGKA"
     gene            1616..2434
                     /locus_tag="VFR57_08865"
     CDS             1616..2434
                     /locus_tag="VFR57_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0259512.1"
                     /translation="MLKTLIGDYPVTRQFRTRTDRFEFAQYQGSVATAFKRVVRNLEF
                     DVAEMAIMTFLVAKAFNKPYRLLPFVVMARYQHPYLVFNAEKTSSLQPEGLEGKRVGV
                     RSYSVTTGAWIRGILMEDHGVDISRIRWVTFEEPHVAEFRDPPNVQRAPEGMDINGML
                     LAGELDAAILGAIPTDPRLKPLIRDPEAAGKRWGEKRRAIQLNHLVGVKNTLSKATAE
                     EVYGLLVESRKAAGDPPTLPHGLEANRRNLEIAIDCAYKQNMIPRRFSVEELLE"
     gene            2431..3381
                     /locus_tag="VFR57_08870"
     CDS             2431..3381
                     /locus_tag="VFR57_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259513.1"
                     /translation="MKRILVSLCFVPVLALAQSYPTKPVRIVLPFGPGGIADITTRTI
                     APKLGEGLGQQVLVENLPGAGGIRAAETVARADPDGHTLLLLTNGNAVSQALFKSLPY
                     DPVNDFAMISTVGFFSMVIVTRPESSAKTLQDAIAMAKSNPGKMNIGTITPGGTQHLA
                     GELFRATAGIDALVVPHKTTGEVIVAARNGNVDLAVDFIAPLLSSIKSGALRPLAVTA
                     GKRFPGLPDVPTVIEAGVPGYDVASWNAMAAPAKTPRAVIDRLNQELRKAIAAPEVQK
                     RFGELGVDARTSTPEELRQFFIAESQRWTRVVETAKIPKQ"
     gene            complement(3394..4086)
                     /locus_tag="VFR57_08875"
     CDS             complement(3394..4086)
                     /locus_tag="VFR57_08875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0259514.1"
                     /translation="MNISYKRPDGKTVNAYLAEAKGAKNAPGVVVIQEWWGLNDQIKG
                     VAEKLAAAGYCALVPDLYRGKVAVEAKEAEHLMTGLNFGDAAGQDVRGAVQHLKATGS
                     RKVGVTGYCMGGALTILAAVHVPEADAAAAWYGFPPLEYVDATKIKAPLQGHWATKDA
                     FFAISKVDELEKKLRDAKVKLEFHRYDAQHAFANETAVGPKRPVAAAEYNAQAAELAW
                     KRTLDFFAKNLK"
BASE COUNT          700 a         1378 c         1337 g          702 t
ORIGIN      
        1 gacgaggcgt gggaccggga gttcctcgag ttgatcgagc gcgatcccga gcgcctggcg
       61 agcatgacgg tcgaagactt catgcagcgc ggcggcaacg agggcgcgga agtcatcatg
      121 tggctcatca tgcgcggcgc gctggacgag cgggtgagaa agctgcacca gaactactac
      181 ctgccgatga cgacggcgat ggccgtggcg ctctttgagt aagtacgtct tcgatctgga
      241 gcgcagcgct cgcgggctgc gcctcaaccg gtttctcaac ggcctgacca aagccgaaaa
      301 ccgcaggctc ttcctcgaga agcccgagga cgcgatgaag gtcctgactg aagaggaaag
      361 gggcatgatc cgccgcctgg actggaaggc gctgcaggac tgcggcgcga gcttcttctg
      421 cctggaaaag ctcgcgcgga ccaaaggggt ttcgaacccc gagatggtcg ccgcgttccg
      481 cggagagacg ctggagcagt tcctcaagac gcgccgggtt ccgggagcgc gctaggcgcg
      541 atgggccctg cctggcacga gcgcatcgcg aaggtgctga aggcgaacga ggtccgcctc
      601 gtgacttaca tcccggacaa cgtgctcgcg ccgctcatca gtgcggtgca cgccgatccc
      661 tggttcaagg cgatctgccc tgcgcgcgag gaggaggccg ccgggatcgt cgccggggct
      721 cacatggccg ggctgcgcgg cattgtgcta atgcagacga gcggcttcgc cacgctgccc
      781 aacgtacttg cctccctcat cgttccgtac cgcattccgc tgctgatgct catctcggag
      841 cgcggcaggc tcggcgacca ccagctcggc caggctatcg tctgcagcac gatgcggccg
      901 gtgctgcaga cgctcggcat cgagcatttc gcgatcgagc ggctcgagga cgtggagttc
      961 gtggccgacg gcatgatccg gcaggcgtat tcgacccagg caccggcggc cgtcgtcctg
     1021 tcgccgctat tgacgaagaa gggcgcatga accgcttcga tctcaccaag cgcctggtgg
     1081 cgaagctgaa agcgaacgag gccgtgatcg gcggcatcgg caacaccaac ttcgatctct
     1141 gggcggcggg ccagcgcccg gagaacttct acatgctcgg cagcatggga cttgcggttc
     1201 cgatcgggct gggcgtggcg ctcgcgcaac ctgcccgccg cacgttcgtg ctcgagggcg
     1261 acggctcgct gctcatgcag ttgggctcgc tcggcaccgt ggcggcgagc gcgcccgcga
     1321 acctcacgat cgtggtattc gacaacggca tgtaccacat caccggcaag cagaagacgc
     1381 tcaccgccgg cacggttgac ctcgtcgccg tggcccgcgc tgcgggcctc gagcgcagcg
     1441 aatgggcgag ggacgaatcg cacttcgaat cgttggtgga ccgcgcgctc cgggaaagcg
     1501 gtcctgcgtt catcggcgcg cgcatcgacg gtgcgagccc cgcgggcgtt accgagcgcg
     1561 accccgctcg catccggcag cgcttcatgg atgctctgtg cgggaaagct tgactatgct
     1621 gaaaacgctg attggcgact atccggtcac tcggcagttc cgtacccgca ccgatcgctt
     1681 cgagttcgcg caataccagg gcagcgtcgc gacggctttc aagcgcgtcg tgcgcaatct
     1741 ggagttcgat gtcgccgaga tggcgatcat gactttcctc gtcgccaagg cgttcaacaa
     1801 gccgtaccgg cttctacctt tcgttgtcat ggcgcgctac cagcatcctt acttggtttt
     1861 caatgcagag aagacttcgt cgctgcagcc ggaaggactg gaaggcaagc gggtcggcgt
     1921 gcgctcttat tcggtgacga ccggcgcctg gatccgcggg atcctgatgg aggaccacgg
     1981 cgtcgatatt tcacgcatcc gctgggtaac gttcgaggag ccgcatgtcg cggagtttcg
     2041 cgatccgccc aatgtgcagc gcgcgccgga gggcatggac atcaacggca tgctgctcgc
     2101 gggcgagctc gatgccgcga ttctcggcgc gatcccgacc gatccgcggc tcaagcccct
     2161 gatccgcgac cccgaggccg ccggcaagcg ctggggcgag aagcgccgag cgatccagct
     2221 caaccatctg gtcggcgtca agaacacctt atcgaaggcg acagcggagg aggtctacgg
     2281 gctgctggtg gaaagcagga aagcggcggg cgacccgccg acgctgccgc acgggctgga
     2341 agccaaccgg cgcaacctcg aaatcgccat cgactgcgcc tacaagcaga atatgattcc
     2401 gcgtcgattc tcggtggagg agctcctcga atgaaaagaa tcctcgtctc tctgtgcttt
     2461 gtccctgtgc tggcgctcgc gcagtcctac cccacgaaac cggtacgcat cgtgctgccc
     2521 ttcggtccgg gcggaattgc ggacatcacc acgcgcacca tcgcgccgaa gctgggcgag
     2581 gggctcggcc agcaggtgct cgtcgagaac ctgcccggag caggcggcat ccgcgcagcg
     2641 gaaaccgtcg cgcgcgccga tcccgacggg cacacgctgc tccttctcac caacggcaac
     2701 gcggtgagcc aggcgctctt caagtcgctg ccctacgacc cggtgaacga cttcgcgatg
     2761 atctccacgg tcggcttctt cagcatggtg atcgtcaccc ggcccgagtc gtccgcgaag
     2821 acgctgcagg acgcgatcgc gatggcgaag tcgaaccccg gcaagatgaa cattggcacc
     2881 atcactcccg gcggcaccca gcatctcgcc ggcgagcttt tccgcgccac cgccggcatc
     2941 gacgcgctcg tcgtgccgca caagaccacc ggcgaagtca tcgtcgcggc gcgcaacggc
     3001 aacgtggacc tcgccgtgga cttcatcgcg ccgctgctct cgagcatcaa gtccggcgcg
     3061 ctgcggccgc tcgcggtcac tgcggggaag cgcttccccg gcctgcccga cgtgccgacg
     3121 gtgatcgagg ccggcgtccc cggctacgat gtcgcgtcgt ggaacgcgat ggcggcgccg
     3181 gccaagaccc cgcgcgcggt catcgatcgc ctgaaccagg agctgcgcaa ggcgatcgcc
     3241 gcgccggaag tgcagaagcg cttcggcgag ctcggcgtcg atgcgcgcac cagcacgccc
     3301 gaagagctac gccagttctt catcgccgag tcccagcgct ggacgcgcgt ggtcgagacc
     3361 gcgaagatcc cgaagcagta gccccgattt tcgttacttg aggttcttcg cgaagaagtc
     3421 gagcgtccgc ttccaggcga gctcagccgc ctgggcgttg tactccgcgg ctgccaccgg
     3481 ccgcttcggg ccgaccgcag tctcgttcgc gaacgcgtgc tgcgcgtcat agcggtggaa
     3541 ctcgagcttt accttcgcgt cgcgcagctt cttctccagc tcgtcgactt tcgagatcgc
     3601 gaagaacgca tccttggtgg cccaatgccc ctggagaggg gccttgatct tcgtcgcatc
     3661 gacatactct agcggcggga acccgtacca cgcggccgcg gcatcggcct cggggacgtg
     3721 gaccgcggcc aggatcgtca gcgcgccgcc catgcagtag ccggtcacgc cgaccttgcg
     3781 gctgccggtc gccttgagat gctggaccgc gccgcgcacg tcctggcccg cggcgtcgcc
     3841 gaagttgagc cccgtcatca ggtgctcggc ctccttcgct tccacggcaa ccttgccgcg
     3901 gtagagatcc ggcacgagcg cgcagtagcc ggcagcggcc agcttctccg ccactccctt
     3961 gatctgatcg ttcagtcccc accattcctg gatcaccacc acgccaggcg cgttcttggc
     4021 gcccttcgcc tcggccaggt aggcgttcac ggttttccca tcgggtcgct tgtagctgat
     4081 gttcatggtt tcctcctgta aatcgggctc tgacccc
//
LOCUS       DASTPD010000269         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_956486, whole genome shotgun sequence.
ACCESSION   DASTPD010000269 DASTPD010000000
VERSION     DASTPD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956486"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFR57_08880"
     CDS             complement(<1..222)
                     /locus_tag="VFR57_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0259515.1"
                     /translation="MQRKLHPLEGIVAICALLFVLFVSVGFAQAQDVRKIALRSSSAL
                     VQDAGTGEVVIGKNADAVVSIASITKLMTA"
     gene            complement(413..2224)
                     /locus_tag="VFR57_08885"
     CDS             complement(413..2224)
                     /locus_tag="VFR57_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0259516.1"
                     /translation="MAHSAAPTQRLRGNEWKAVATLLPYLWEYKGRVFLALACLVSAK
                     LANVGVPLVMKEVVDGLDPSLQIVAVPVALLAIYGLLRFSTTLFQELRDVVFVRVTQR
                     AIRRVALGVFRHLHSLSLRFHLERQTGGMTRDIERGTRGISTLLSYLIFSILPVILEF
                     ALVAAVLVARFDWRFAAITFGAVVVYLFFTVMITEWRMEIRRRANELDSRANTRAIDS
                     LLNYETVKYFGNEEFEVRRYDEQLHKYEDAATKNEASLGILNIGQSLIIAIAVTALML
                     LVAEGVAAKSFTIGDLVLVNGLLIQLYIPLNFLGMVYREIKQALLDMDRMFRLLAEHR
                     EVEDRPGAPELPAGPATVEFKDVEFSYEKERQILHRVSFTIPAGHRVAVVGHSGSGKS
                     TLARLLYRFYDVSAGRIEINGVDIRQVKQASLRAAIGIVPQDTVLFNDTILYNIRYGR
                     PEASDAEIYEAARAAQIHRFIESLPKGYEAMVGERGLKLSGGEKQRVAIARALLKNPR
                     ILIFDEATSALDSRAEKAIQAELERIAQGRTTLVIAHRLSTVMDADQILVLVQGRIAE
                     RGTHAQLLDVKGEYARMWALQQQQAQAQEILESTAAE"
     gene            2290..>3268
                     /locus_tag="VFR57_08890"
     CDS             2290..>3268
                     /locus_tag="VFR57_08890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0259517.1"
                     /translation="MAPHALPEGYALQEYRIQKVLGVGGFGLTYLAVDSNLNLKVAVK
                     EYLPVDVAQRGDDQTIGPRSPSTAETFGWGKQRFMDESRTVASFRHPNIVRVMRFFEA
                     NGTAYMVMEFVEGAALAEWIKARRPLAEAQVSAIVEPLLEGLEVIHNAGYLHRDIKPG
                     NIYIREDGTPVLLDFGSARQRATELTAVVSPGYAPFEQYHTQGKQGPWSDIYALAGVL
                     YWMVTGSMPLEAPARVRADEMAAAVQAAERSRYRPEFLAAIDWALAPFEDQRPQSVAE
                     WREALLPSKPVEVKPQKVDTAPPAPRVPAEVLKRAEERLAEYIGPVAQEV"
BASE COUNT          587 a         1054 c         1051 g          576 t
ORIGIN      
        1 cgccgtcatc agcttggtga tggacgcgat cgacaccacc gcatccgcgt tcttgcctat
       61 cacgacctcc ccggtcccgg cgtcctgaac gagcgccgaa gaggagcgca gcgcgatctt
      121 gcgtacatcc tgggcctgcg caaatccgac gctcacgaaa agcacaaaaa gcagagcgca
      181 gatcgcgacg atcccctcta gcgggtggag ttttctttgc atgttgtgat cttccccctc
      241 gatccgcagt ggtttttctt tgcgtcccat gccctgaacg gcacatggac tgccggagtc
      301 tgcctacggc gccaaaaaaa tgcaaactaa aacagtggct agcacaaaat ttcaagaaat
      361 cgcgtgagat tgtccacttg cggcgacagc agcgccgacg ggcgggcgcc ggctattccg
      421 cagcggtgct ttcgaggatt tcctgggcct gcgcctgctg ctgctggagc gcccacatgc
      481 gggcgtattc tcctttaaca tcaagcagtt gcgcgtgcgt tccgcgctcg gcaatgcgac
      541 cctggacgag caccaggatc tggtcggcgt ccatcacggt agacagccgg tgcgcgatga
      601 cgagggtggt gcgcccctgc gcgatgcgct ccagttcggc ctggatcgcc ttctcggcgc
      661 gcgagtcgag cgccgaggtc gcctcgtcga aaatgaggat gcgcggattc ttcaggagcg
      721 cgcgtgcgat ggcgacgcgc tgcttttctc cacccgagag cttcaggccg cgctcgccca
      781 ccatcgcttc atagcctttg ggcagacttt caataaaccg gtgaatctgc gcagcgcgcg
      841 ctgcctcgta gatctccgcg tcgctcgcct cgggccttcc gtagcggatg ttgtagagaa
      901 tagtgtcgtt gaagagcacc gtgtcctgcg gcacgatgcc gatcgccgcg cgcaggctcg
      961 cctgcttcac ctgcctgatg tcgacgccgt tgatctctat tcttcctgcg gaaacatcgt
     1021 agaaacggta gagcagccgc gcgagcgtcg acttgcccga gcccgagtga ccgaccaccg
     1081 ctacgcggtg gccggcggga atcgtgaagg acacgcggtg aaggatctgc cgttcctttt
     1141 cgtaggagaa ctctacgtcc ttgaattcaa ccgtagcggg ccccgccggc agctcgggtg
     1201 cgcccgggcg gtcctcgacc tcgcgatgct cggcgagcag ccggaacatg cggtccatgt
     1261 cgagcagcgc ctgcttgatc tcgcgataca ccatgccgag gaagttgagc ggaatgtaga
     1321 gctggatgag aagtccgttc accagcacga gatcgccgat ggtgaagctt ttcgccgcca
     1381 cgccctcggc gacgagcagc atgagcgccg tcaccgcgat cgcgatgatc aggctctgcc
     1441 cgatgttgag gatcccgagc gaggcctcgt tcttggtcgc ggcgtcctcg tacttgtgca
     1501 gctgctcgtc gtagcgccgc acttcgaatt cctcgttgcc aaagtacttc accgtctcgt
     1561 agttgagcag gctgtcgatc gcgcgcgtgt tcgccctgga gtcgagctcg ttggcgcggc
     1621 gccggatctc catgcgccac tcggtgatca tgacggtgaa gaagaggtac accacgaccg
     1681 cgccgaaggt gatcgcagcg aagcgccagt cgaatctcgc aaccagcacg gccgcgacca
     1741 gcgcgaactc gaggatcacc ggcaagatgg agaagatcag gtacgacaga agcgtggaga
     1801 tgccgcgcgt cccgcgctcg atgtcgcgcg tcatgccccc ggtctggcgc tcgaggtgaa
     1861 agcgcagcga caggctgtgc aggtggcgaa acacgccgag ggcgacgcgc cggatggcac
     1921 gctgcgtcac gcgcacgaag accacgtcgc gcagctcctg gaacagcgtg gtcgagaagc
     1981 gcagcagccc gtagatcgcg agcagcgcca ccggcaccgc gacgatctgc aggctcgggt
     2041 cgagtccgtc caccacctct ttcatgacga gcggcacacc gacgttggcg agcttcgcgc
     2101 tcaccaggca ggcgagcgcg aggaagaccc gccccttgta ttcccacagg tagggaagaa
     2161 gggtggccac ggccttccat tcgttgcccc tcaatcgctg cgtcggagcg gcgctatgtg
     2221 ccatatgccc ggattatcgc aatgtcggtt atgccgaagg cccggactta cgtatagttg
     2281 ccgccaagaa tggcgccgca tgcactgccc gagggctatg ccctccagga atatcgcatt
     2341 cagaaggtgc tcggcgtggg cggcttcggc cttacctacc tcgcggtcga ctccaacctg
     2401 aacttgaagg tggcggtaaa ggagtacctg ccggtcgacg tagcgcagcg cggcgacgac
     2461 cagacgatcg ggccgcgctc gccctcgacc gccgagactt tcggctgggg caagcagcgc
     2521 ttcatggacg aatcgcgcac cgtggcttcg ttccgccacc cgaatatcgt gcgcgtgatg
     2581 cgcttcttcg aggcgaacgg cacggcgtac atggtgatgg agttcgtcga gggcgcagcg
     2641 ctcgctgaat ggatcaaggc ccgccgcccg ctcgccgagg cgcaggttag cgctatcgtc
     2701 gagccgctcc tcgaggggct cgaggtcatc cacaacgccg gctacctgca ccgcgacatc
     2761 aagcccggca acatctacat ccgcgaggat ggcaccccgg tgctgctcga cttcggctcg
     2821 gcgcggcagc gcgcgaccga gctcaccgcg gtggtttcgc ccggctatgc gcctttcgag
     2881 cagtaccaca cgcagggcaa gcaggggccg tggagcgaca tctacgccct tgccggggtt
     2941 ctctactgga tggtgaccgg ttcgatgccg ctcgaggcgc cggcgcgggt acgtgccgac
     3001 gagatggccg cggcggtcca ggccgctgag cgcagccgat accggcccga attcctcgcg
     3061 gcgatcgact gggcgctcgc gcccttcgag gatcagcgcc cgcagtcggt tgccgaatgg
     3121 cgcgaggcct tgctccccag caaacccgtt gaagttaaac cacaaaaggt cgatacggcg
     3181 ccgccggcgc cgcgcgtgcc ggccgaggtg ctgaagcgcg cggaggagcg gctcgccgag
     3241 tacatcgggc cggtggcaca ggaggtgg
//
LOCUS       DASTPD010000270        10687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1365984, whole genome shotgun sequence.
ACCESSION   DASTPD010000270 DASTPD010000000
VERSION     DASTPD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365984"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFR57_08895"
     CDS             <1..484
                     /locus_tag="VFR57_08895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU0259518.1"
                     /translation="CEELMPQSMKIRITSIADLPLFNQDVFDAGIPEPCKRLRGEIAQ
                     ADGVLIASPEYNFSVTAPLKNAIDWASRPPNQSWQDKPVALFSVTPGPLGGARVQYDL
                     RRILGQLWAHVLPRPEVFIGSSATKFADGKLTDETTRKFLADLLAGFKPWIERMRVRK
                     "
     gene            547..1356
                     /locus_tag="VFR57_08900"
     CDS             547..1356
                     /locus_tag="VFR57_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259519.1"
                     /translation="MIRALLAVLLALSTLSGKAHADEGWTFSLTPYLWLPNVNGTLKY
                     AVPPGGGARPEVDTGPNDYLENLSFALMLAGEARKGRWSMFTDLIYLDFDSEESTVKT
                     VNFGGERIDTTLNSSTRSSLTGVAWTFGGSYAALQDPKHRLDVLGGVRYFGLKAASDW
                     QLSAAFSGPNGQVLAQSGSISQKEDLWDVIVGVRGRLKLGEGSWFVPYYLDVGTGSSA
                     LTAQALAGIGYAFKWGEILATYRTVYYDLDEDGLLQDVRFAGPAIGATFRF"
     gene            1368..1922
                     /locus_tag="VFR57_08905"
     CDS             1368..1922
                     /locus_tag="VFR57_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0259520.1"
                     /translation="MPKIFSLLVLAFGSLSVSQLHAADGLYLGAGVGAASVKDSFSTE
                     TFDEEDVAYRAFVGWRFDRVPIIDLAVEAAYTNFGKPSQTIVQGGAAQNVEVKLHGAS
                     LAGLLILPLGPIDLYAKGGVIEWKADQTVNGSTTGRSGTDAFYGAGIGFSLWILAFRA
                     EYERFQIKEVDRVQMLSVSALFQF"
     gene            complement(1931..2641)
                     /locus_tag="VFR57_08910"
     CDS             complement(1931..2641)
                     /locus_tag="VFR57_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259521.1"
                     /translation="MLRLSEVHASYGAATVLHGVSIEMGAGETVALLGPNGAGKSTLL
                     AAVTGTIRSRSGSIVFDGAEIVRAPAHRIVQAGMRLVPEGRLVFSPFSVEDNLRLGAL
                     RLEGELSERFDYVYRLFPRLAERKEQPAGTLSGGEQQMLAIGRALMSRPRLLLLDEPF
                     LGLAPLVVHQIRSALEKLRASGLTLLIVEQKLDIALAFASRAYVLIKGAVVLHDTTAD
                     LVRRANLAELYLSLAPRG"
     gene            complement(2625..3380)
                     /locus_tag="VFR57_08915"
     CDS             complement(2625..3380)
                     /locus_tag="VFR57_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259522.1"
                     /translation="MTDILEARKLTKRFGGLTAVSELSFAVREREILGLIGPNGAGKS
                     TTFNIIAGTYRADSGEISFLREPIAGLPPHEIARRGVMRTFQHNLPFEGMSVLENVLV
                     GAHTRFGGNLFSRKAAAEEREQHAHAERLVEFVGLGELRYASVDRLSFGQGRLLEIAR
                     ALAGAPKLILFDEPAAGLTRAEAQRIATVIRGIAERGIAVLLIEHDMHFLLPLAHRVV
                     VLNFGAKIAEGTPDEVRSHPAVLDAYLGHAAAV"
     gene            complement(3346..4278)
                     /locus_tag="VFR57_08920"
     CDS             complement(3346..4278)
                     /locus_tag="VFR57_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259523.1"
                     /translation="MRSALGWVAAAVLLSAVAFFLSGYYNDVYRKLLLWIALALSYNF
                     LFGISGQVAFSHFAFYGIGAYAVVIFLFQLGMPLPLAILAGVLVCSAIALIVAIPSTR
                     LEGFYLALATLALAQLFIVVLNEGGAVTGGTGGLVNYRLPAIFGVYITGPWYTAVIVI
                     LVLGTYALLRHLDRSWFGRACRAVRDNPEAAAAMGVNVARTKVIAFTLTSVLAGIAGM
                     VYAFVDNTVNPPIFGLENAFLLLFMVIIGGSGRHAGAIVGAVLLYLLPFVLSPLIGHH
                     HALVFGALMVVVILLQPRGLIGLYDRYPRGQKAH"
     gene            complement(4275..5120)
                     /locus_tag="VFR57_08925"
     CDS             complement(4275..5120)
                     /locus_tag="VFR57_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259524.1"
                     /translation="MRDLLELSVSAAASGCIYALVALAYLLMIRPTGVINFAVGEWAM
                     VGAFGGFLLLSKYEWPYALGMAAVLGFMLLVGWITERIAVRPLVERGAPLLAAILALL
                     GMLVVFREGISLGFGPDPHQVPEPVSFEYLKLFVIGATLVVFAAVWLFFERTLWGKAF
                     EAVALNRRAAALMGIDLRRVTVASFAGGAAAAGLAGLLVAPNTSAHYLMGVPLAIQGF
                     TALVIGGVGRVEGALLGGLLVAFAEQLTVRYAPLPSGYAQGVPLLLLMLFLLVRPTGL
                     LRARA"
     gene            complement(5121..6248)
                     /locus_tag="VFR57_08930"
     CDS             complement(5121..6248)
                     /locus_tag="VFR57_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259525.1"
                     /translation="MRKWLAALLAAVSASVVAQETIKIGVITDKVGPAKPYSEPTLEG
                     IQFGANEINAAGGVLGRKIELLIEDDQGRPDISAAMARKLVDAGAVFILSITLTPATQ
                     QQQNVTLEAKTPQMTPMNSGDTLTTQLQNPYFWQTGPLGSIQIATLLSHARSKQLKRV
                     ALITDNSDLGQLLAKFFRAGLEKSGIQVVAEEVVPRGATTAEPQMQKVRAANPEAMFM
                     AGVLTPENVLILRAYRQMALKFPIHSSYNLSVPQYMSVAKGLLNGITFVDAYDPEKPE
                     VKKFEADYRKATGKLPYNIHGYGYDGIKMVAEAIKRAGSADKEKIRAAMQSLEFSGVM
                     GAKGMKYVFPDGKRAGFDPNGMVVRVYEGDKQGRVVHVGQK"
     gene            complement(6259..7413)
                     /locus_tag="VFR57_08935"
     CDS             complement(6259..7413)
                     /locus_tag="VFR57_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259526.1"
                     /translation="MSFEVAPEWVHVQYQESAKFTEVYGFAGSQGTVNCEGVRFVPKG
                     KPSRTLAVFMHPSSTLQLLPMPQALAKAGLHVLCAGSRFARNDTPLIMEKVLLDLGAY
                     MRRAKEQWGYEKVVLCGWSGGGSLALFYQSQAERPSITATPAGDPVEIRNLIPADGMI
                     FQAAHLSRARMLSEWIDPSVVDEGNPDERDLELDIYNAKCPNQPPYSRQFIERFRSAQ
                     LSRMRRRTQWVKQTLEKLKGDELERGFVTYRTMCDVRFMDPAVDPNDRKPRWCYLGRP
                     ETANTGPVGIGRFSTLRAWLSQWSVDDSNADGPTCARSIGVPLLAIENSADDAVPQPH
                     ARIIHDAAGSRDKTFRVIQGATHYYQGQPELLQQAVEVCVGWMAKRNLLD"
     gene            complement(7410..7934)
                     /locus_tag="VFR57_08940"
     CDS             complement(7410..7934)
                     /locus_tag="VFR57_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259527.1"
                     /translation="MDIKSLHHVAYRCKDAKQTADFYTKVLGLEYAMAMAEDRVPSTG
                     EASPYMHIFFRMGDGSYVAFFEVPESPPMGRDANTPAWVQHLALRVPDEETLMSYKAK
                     LEKLGIEVVGPTDHTICKSIYFFDPNGHRLELTVDTTTPEMKKRLAAVSEEMLAEWSK
                     TKKAPHHADWVHKK"
     gene            7964..8350
                     /gene="pcaC"
                     /locus_tag="VFR57_08945"
     CDS             7964..8350
                     /gene="pcaC"
                     /locus_tag="VFR57_08945"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEU0259528.1"
                     /translation="MDKREHKQRYDAGLKTRREVLGPAYVDKAMAGVDEFSQEFVELL
                     NTYCWNDIWNRPGLPRKTRSILNLGMLCALGKEHELKLHIRGAINNGLTKEEIKEVFL
                     QVAIYCGVPAAVVAFRCAKEVFQSEK"
     gene            8371..9186
                     /locus_tag="VFR57_08950"
     CDS             8371..9186
                     /locus_tag="VFR57_08950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259529.1"
                     /translation="MGKLMAARLDKAYAVQSYDLNGKGNCDSAREAAWGADALITMLP
                     DGDAVRDAVLDALPSLRPGAVVVDMSSSDPAATLALGRALRARRVDMVDAPVSGARFK
                     AKDGTLAIMVGGEKKIVRRVMPLLQRLGTEIFHVGGHGAGHAVKALNNYLGAAGTLAG
                     FEALLIAQKFGLNPKPMIAAINASTGRNSTTARKIPQDVLTGRFASGFKLALMTKDVA
                     IAGDLAKTLRVNTPYLRETLKHWRAAQKQLPRNADHTEIYKYQKRVLSSAKRR"
     gene            complement(9173..9961)
                     /locus_tag="VFR57_08955"
     CDS             complement(9173..9961)
                     /locus_tag="VFR57_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0259530.1"
                     /translation="MPSEKLIAKKENGVGWIVLNNPERHNAISLEMWTAAMEAMDDFR
                     ADKSVRVVVVTGAGGKAFASGADISKFKDERQEAAAVAHYQATTQKAYDTVLGMPIPT
                     IAMIRGYCIGGGTALAVCCDIRICTENAKFGVPAAKLGLGYGMNRAKPLVDLIGPAYA
                     KEMFFTGRQFTAQEAEKMGLVNRVVADEQLETVVGEMARTIVENAPLTVKCAKLVVGE
                     VLKDPENRDVGATERAVEACFKSQDYKEGQAAFMEKRKPRFIGA"
     gene            complement(9961..>10687)
                     /locus_tag="VFR57_08960"
     CDS             complement(9961..>10687)
                     /locus_tag="VFR57_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0259531.1"
                     /translation="MSITGLPGQGPVRVGIPIADLCAGIFCAYGIAVALIEREKSGKG
                     QWVKSSLLQAQLFMLDFQASRWLMKGEVAKQAGNNHPTSIPTGVYKTRDGYMNLAVTG
                     TKIWTRMCEAIGVPELATHPQYSSSSLRSQNRDALHEVLEEYFVKKDTAYWIETLNDA
                     GVPAGPIYSVDQAFNDPQVKSLGMVQNVGQVPYLGQPVTLSRTPSKVAAHPPKQGEHT
                     DQVLQEVGFSKDKIEELRKKGIV"
BASE COUNT         1949 a         3387 c         3481 g         1870 t
ORIGIN      
        1 gtgcgaggag ctcatgccgc agagtatgaa gattcgcatt accagcatcg ccgacctgcc
       61 ccttttcaac caggacgttt tcgacgccgg catcccggag ccgtgcaagc gcctgcgcgg
      121 ggaaatcgcg caggccgacg gtgttctgat cgcaagcccg gagtacaact tttcggtcac
      181 cgcaccgctc aagaacgcga ttgactgggc ctcgcggccg ccgaaccagt cctggcagga
      241 caagccggtg gcgctttttt cggtgacgcc gggcccgctg ggcggcgcgc gcgttcagta
      301 cgacctgcgc cgcattcttg gccagctgtg ggcgcacgtg ctgccaaggc ccgaagtgtt
      361 catcgggtcc tccgcgacca agttcgcgga tggaaaactc accgatgaga ccacgcgcaa
      421 gttcctcgcc gacctgctcg ccggcttcaa gccatggatc gagcggatgc gcgtaaggaa
      481 gtagccgcgc cgattgaact aaggttcgat ggctggctgc gcacagcggc cctagaatcc
      541 gccgccatga tccgagccct gttggcagta ctgctggcgc tcagcacgct ctcgggcaag
      601 gcgcacgcgg acgaaggttg gaccttttcg ctgacgccct acctctggct gccgaacgtg
      661 aatgggacgc tcaagtatgc cgttccgccg ggcggcggcg cgaggcccga ggtggacacg
      721 gggccgaatg actacctcga gaacctcagc ttcgcgctca tgctcgccgg agaggcgcgc
      781 aagggccggt ggtcgatgtt caccgatctc atctacctcg attttgacag cgaggaaagc
      841 acagtgaaga ccgtgaactt cggcggagag cggatcgaca cgaccttgaa ttcgagcact
      901 cgatcatcgc tcactggcgt ggcgtggacc tttggtggca gctacgccgc gctgcaggac
      961 ccgaagcata ggctcgacgt gctgggcggc gtgcgctatt tcggcctgaa ggctgcgagc
     1021 gactggcaac tcagcgccgc gttcagcggt cccaatggcc aggtgttggc gcagtcgggc
     1081 agcatctccc agaaggagga cctttgggat gtgatcgtcg gcgttcgcgg ccgcctgaag
     1141 ctcggcgaag gtagctggtt cgttccgtat tacttggacg tcggcaccgg ctcctctgcg
     1201 ctgactgcgc aggcccttgc ggggatcggc tacgccttca agtggggcga gattctcgcc
     1261 acctatcgca ccgtctacta cgatctcgac gaagacggac tgctgcagga cgtgcgcttt
     1321 gccggcccgg ccatcggcgc aacctttcgc ttctagaagt gccttccatg cccaagatct
     1381 tctcgctgct cgtgctggca ttcggaagct tgagcgtttc gcagttgcat gcggctgatg
     1441 gactgtacct tggcgccggc gtgggcgccg ccagcgtcaa ggacagcttc agtaccgaga
     1501 cattcgacga agaggacgtg gcctacaggg cgttcgtagg ctggcgtttc gaccgagtgc
     1561 cgatcatcga cctcgccgtc gaggcggcct atacgaattt cggcaagccg tcccagacca
     1621 tcgtgcaggg tggagcggcg cagaacgtcg aggtcaagct gcacggcgcg agtctggccg
     1681 gtctgctgat tctgccgctc ggaccgatcg atctctatgc gaaaggcggg gttatcgagt
     1741 ggaaagccga ccagacggtc aatggttcga ccaccgggcg gtccggaacc gatgcctttt
     1801 acggcgccgg gatagggttc agtctgtgga tactggcctt tcgtgccgag tacgagcgct
     1861 ttcagatcaa ggaagtcgat cgcgtgcaga tgctttcggt gagcgccctg ttccagtttt
     1921 gagttgccgg tcaaccgcga ggcgccagcg agaggtagag ctcggcaagg ttggcgcggc
     1981 gcactaggtc tgcggtggtg tcatgcagca ccaccgcgcc cttgatgagc acgtaggcgc
     2041 ggctcgcgaa cgcaagcgcg atgtcgagct tctgctcgac gatcagaagc gtgagcccgc
     2101 tcgcgcgcag cttctccagc gcgctgcgaa tctgatgtac gacgagcggc gcgagcccga
     2161 gaaaaggctc gtcgagaaga agcagccgcg gccggctcat gagcgcgcgg ccgatggcga
     2221 gcatctgctg ctcgccgccc gagagcgtgc ccgcgggctg ttccttcctt tcggcgagac
     2281 gcggaaaaag gcggtacacg tagtcgaagc gctcgctcag ttcgccttcg agccgcagcg
     2341 cgcccaggcg caggttatcc tcgacgctga acggcgagaa gacgagccgg ccctccggca
     2401 caaggcgcat accggcctgc acgatgcggt gcgccggcgc gcgcacgatc tcggcgccgt
     2461 cgaagacgat gcttccgctg cggctacgga tcgttcccgt gaccgctgcg agcagcgtgg
     2521 actttccggc gccgttcggc ccgagcagcg ccaccgtctc gcccgcgccc atctcgatgc
     2581 tgacgccgtg cagcaccgtg gcggcaccgt aggaagcgtg gacctcagac agccgcagca
     2641 tggccgagat acgcgtccag gaccgccgga tgcgagcgca cttcatcggg cgtgccttcg
     2701 gcgatcttcg cgccgaaatt gagcaccacc acgcggtgcg cgagcggcag caagaaatgc
     2761 atgtcgtgct cgatcagcag aaccgcgatg ccgcgctcgg cgatgccgcg aatcaccgta
     2821 gcgatgcgct gggcttcggc gcgcgtcagg ccggcggcgg gttcgtcgaa gagaatcaac
     2881 ttgggcgcgc cggcgagcgc gcgggcgatc tccagcagac ggccttgccc gaacgagagc
     2941 cggtccacgc tcgcgtagcg cagctcgccc aggccgacga attccaccag gcgctcggcg
     3001 tgcgcgtgct gctcgcgctc ctcggccgcc gccttgcgcg aaaaaagatt cccgccaaag
     3061 cgcgtgtgcg cgccaaccag cacgttctcg agcacgctca tgccttcgaa cggaagattg
     3121 tgctggaagg tgcgcatcac gccacgccgg gcgatctcgt ggggcggcag cccggcgatc
     3181 ggctcccgga gaaaggagat ttcgccggag tcggcgcgat acgtgccggc gatgatgttg
     3241 aacgtcgtgc ttttcccggc gccgttgggg ccgatcaggc cgaggatttc ccgctcgcgc
     3301 acggcaaagc tcagctccga gaccgcggtc aggccgccga agcgcttagt gagctttctg
     3361 gcctcgagga tatcggtcat agagcccaat taggccgcgc ggttgaagaa ggatgacgac
     3421 gaccatcagc gcgccgaaga cgagcgcgtg gtggtggccg atgagcggcg agagcacgaa
     3481 aggcagaagg taaagcagca ccgcgccgac gatcgcgccc gcgtggcgcc ccgagccgcc
     3541 gatgatcacc atgaaaagca agaggaacgc gttctcgagg ccgaagatcg gtggattcac
     3601 cgtgttgtcc acgaacgcgt acaccatgcc tgcaatcccg gcgagcacgc tcgttagcgt
     3661 gaaggcgatg actttggtgc gcgccacgtt cacacccatg gccgcggcgg cttccgggtt
     3721 gtcgcgcacc gcgcggcacg cgcggccgaa ccacgagcgg tccaggtgcc gcagcagtgc
     3781 gtacgtgccc aggacgagga ttacgatgac cgcggtgtac cagggcccgg tgatgtacac
     3841 gccgaagatc gccggcaggc gatagttgac gaggccgccg gtgccgccgg tgaccgcgcc
     3901 gccctcgttc agcaccacga tgaaaagctg ggcgagcgcg agcgtggcga gcgccagata
     3961 aaagccctcg aggcgcgtcg acgggatggc cacgatgagc gcgatagcgc tgcagaccag
     4021 gacgccggcc aggatggcga ggggcagcgg catgcccagc tggaacagga agatgaccac
     4081 cgcgtatgcg ccgatgccgt agaaggcgaa gtgcgagaag gccacctgcc cggagatgcc
     4141 gaagaggaag ttgtaggaaa gcgcgagcgc gatccacagc aagagcttcc ggtaaacgtc
     4201 gttgtaatag ccgctgagga agaaggccac agccgaaagc agcaccgccg ccgccaccca
     4261 gccgagcgcg ctcctcatgc gcgagcgcgc aaaaggccgg tgggccgcac gagcagaaaa
     4321 agcatgagga gaaggagcgg cacaccttgc gcatagcctg agggcagcgg cgcgtagcgc
     4381 accgtgagct gctctgcgaa ggcgacgagc aatccgccga ggagcgcacc ttccactcgc
     4441 cctactccgc cgatgaccag ggccgtgaaa ccctggatcg cgagcggcac gcccatgagg
     4501 tagtgcgcgg aggtgttagg cgcgaccaac agccccgcga gcccggcggc ggccgcgccg
     4561 ccggcgaacg acgcaactgt gacgcgccgc agatcgatgc ccatgagcgc cgccgcgcgg
     4621 cggttgagcg ccacggcctc gaacgccttg ccccatagcg ttctctcgaa gaaaagccac
     4681 actgccgcga acacgacgag cgtcgcgccg atcacgaaga gcttgaggta ttcgaagctg
     4741 acgggctcgg gcacctggtg cggatcagga ccgaagccga gggagatccc ttcgcggaag
     4801 acaacaagca tgccaagaag cgcgaggatc gcggcaagga gcggcgcgcc acgttccacc
     4861 agcggccgta ccgcgatgcg ttcagtgatc caaccgacga ggagcatgaa gccgaggacc
     4921 gccgccatgc cgagcgcgta cggccattcg tacttcgaga ggaggaggaa cccgccgaaa
     4981 gcaccgacca tcgcccactc gccgaccgcg aaattgatga ccccggtcgg gcggatcatc
     5041 aagagatatg cgagagctac gagcgcatag atgcagcccg acgcggccgc gctgacggag
     5101 agctctaaaa ggtcacgcaa tcacttctgg ccgacgtgga ccacgcggcc ctgcttgtcc
     5161 ccttcgtaca cacgcaccac catgccgttc gggtcgaagc cggcgcgctt gccgtccggg
     5221 aacacgtact tcatgccctt cgcgcccatt acgccggaga actctagaga ctgcattgcc
     5281 gcgcgaatct tttccttgtc ggccgagccg gcgcgcttga tcgcctcggc gaccatcttg
     5341 atgccgtcgt agccgtaacc gtggatgttg tagggcagct tgccagtggc cttccggtag
     5401 tcagcctcga atttcttcac ctcgggcttt tccgggtcgt acgcgtcgac gaacgtgatg
     5461 ccgttcagga gacccttcgc gaccgacata tactggggca ccgacaggtt gtaagaagaa
     5521 tgaatcggga acttgagcgc catctgccgg tacgcacgca ggatgagcac gttctcggga
     5581 gtgagcacgc ccgccatgaa catcgcttcc gggttggcgg cacgaacctt ttgcatctgc
     5641 ggctcggccg tggtggcgcc gcgcggcaca acttcctcgg cgaccacctg gatccccgac
     5701 ttctcgagac cggcgcggaa aaacttcgcg aggagctggc cgaggtcgga gttgtcggta
     5761 ataagcgcca cgcgtttgag ctgtttggat cttgcgtgag acaggagcgt cgcgatctgg
     5821 atcgagccga gcggcccggt ctgccagaag tacggattct gaagctgcgt ggtcagcgtg
     5881 tcgccggagt tcatgggcgt catctgcggc gtcttcgctt cgagcgtcac gttctgctgc
     5941 tgttgcgtcg ccggcgtcag cgtgatgctc aggatgaaca ccgcgccggc atcgaccagc
     6001 ttgcgcgcca tcgcggccga aatgtccggg cggccctggt cgtcctcgat aaggagctcg
     6061 atcttgcgtc ccagcacgcc gccggcggcg ttgatctcgt tcgcgccgaa ctggatgccc
     6121 tcgagcgtcg gctccgaata cggcttcgcc gggcccacct tgtcggtgat aacgccgatt
     6181 ttgatggttt cctgcgcaac aaccgacgcg gacaccgcgg caagcagtgc cgccagccac
     6241 ttcctcatga ccccctcctc agtcgagcag attgcgcttc gccatccatc ctacacaaac
     6301 ttccaccgcc tgctgcagca gctcgggctg cccctggtag taatgggtcg cgccctggat
     6361 cacgcggaac gtcttgtccc ggctgccggc agcgtcgtgg atgatgcgcg cgtgcggctg
     6421 cggcaccgca tcgtccgccg aattctcgat cgccaggagc ggcacgccga tggagcgcgc
     6481 gcacgtcggc ccgtcggcgt tcgagtcgtc gactgaccac tgcgagagcc aggccctgag
     6541 cgtcgaaaac cgaccgatgc ccaccggccc ggtattcgcg gtttcgggtc ttccgagata
     6601 gcaccagcgc ggcttgcgat cgtttggatc caccgcggga tccatgaagc gcacatcgca
     6661 catcgtgcga tacgtgacga agccgcgctc cagctcatcg cccttcagct tttccagggt
     6721 ttgctttacc cactgcgtcc gccgacgcat ccggcttaac tgcgcagagc ggaagcgctc
     6781 gatgaattgc cgggagtaag gcggctggtt cgggcacttc gcgttgtaga tgtcgagctc
     6841 gagatcgcgc tcgtccgggt tcccctcgtc gactaccgaa ggatcgatcc actccgaaag
     6901 catgcgcgca cgtgagaggt gcgcagcctg gaagatcatg ccgtccgcgg gtatcagatt
     6961 tctgatttcc accgggtcgc cggcgggcgt cgccgtgatg gatgggcgtt cggcctgcga
     7021 ctggtagaaa agcgcgagcg agccgccacc cgaccagccg cagagcacga ccttctcgta
     7081 cccccactgc tctttcgcgc gccgcatgta ggcgccgaga tcgaggagca ccttctccat
     7141 gatgagcggc gtatcgttgc gcgcgaagcg cgagcccgcg cacagcacat gaagtcccgc
     7201 cttggcgagc gcctgcggca tcggcaggag ctgaagcgtg gacgacggat gcatgaagac
     7261 ggcgagcgtg cgcgagggct tcccctttgg cacgaaacgc acgccctcgc aattcacggt
     7321 gccttgtgag ccggcgaagc cgtagacctc cgtgaacttc gccgactctt ggtactgcac
     7381 atgcacccat tcgggtgcaa cttcaaagct cacttcttat gcacccagtc ggcgtggtgc
     7441 ggggccttct tggtcttcga ccattcggcg agcatttcct cgctcaccgc ggcgaggcgc
     7501 tttttcattt ccggcgtcgt cgtgtcgacg gtaagctcga ggcggtgccc gttcgggtcg
     7561 aagaaataga tcgacttgca gatcgtgtgg tccgtggggc cgacgacttc gatgccgagc
     7621 ttctcgagct tggccttgta gctcatcagc gtttcttcgt cgggcacgcg cagcgcaagg
     7681 tgctgcaccc aggccggcgt attggcgtcg cgccccatgg gcggcgactc gggtacttcg
     7741 aagaaagcga catagctgcc atcgcccatg cgaaagaaga tgtgcatgta cggcgacgct
     7801 tcgcccgtgc tcggcacacg gtcctcggcc atcgccatcg cgtattcgag tcccagcacc
     7861 ttcgtataga agtccgcggt ctgtttcgcg tccttgcagc ggtacgcgac gtggtgaagg
     7921 gatttaatgt ccataatggg gattatcttc ccaaggagaa tccatggaca agcgcgagca
     7981 caagcaacgc tatgacgccg gcctgaagac gcggcgcgag gttctcggac ccgcttatgt
     8041 cgacaaggcg atggcgggcg tcgacgagtt cagccaggag ttcgtcgagc ttctcaacac
     8101 ctattgctgg aacgacatct ggaaccggcc cggccttccg cgcaagacaa gatccatttt
     8161 gaacctgggc atgctgtgcg cgctcggcaa ggagcacgag ctgaagctcc acatccgcgg
     8221 cgcgatcaac aacggcctga cgaaggaaga gatcaaggaa gtgtttctcc aggtcgccat
     8281 ctattgcggc gtgccggccg cggtggtcgc gttccgttgc gcgaaggaag tgttccaatc
     8341 cgaaaagtag cattcatcgg cctgggcgtc atggggaagc tcatggcggc gcggctggac
     8401 aaggcatatg ccgtgcaatc gtacgatctc aacggcaaag gcaactgcga ctcggcgcgc
     8461 gaagcggcct ggggcgccga cgcgctcatc accatgctgc cggacggcga cgcagtgcgc
     8521 gatgcggtgc tcgacgccct gccctcactc aggccgggag cggtcgtcgt cgacatgagc
     8581 tcgtccgacc ccgccgcgac gctcgctctc ggacgcgcgc tgcgcgcgcg gagggtcgac
     8641 atggtcgatg ccccggtctc cggcgcgcgc ttcaaagcca aggacggcac gctcgccatc
     8701 atggtgggcg gcgagaagaa gatcgtaagg cgcgtgatgc cgctcctgca gaggctcggc
     8761 accgagattt tccacgtcgg cgggcatggc gcgggacacg cagtgaaagc gctcaacaac
     8821 tatcttggcg ccgccggcac gctggcggga ttcgaggcgc tcctcatcgc gcagaagttc
     8881 ggactgaatc cgaagccgat gatcgccgcg atcaacgcct cgaccgggcg caacagcacc
     8941 acggcgcgca agatcccgca ggacgtactc accggccgct tcgcctcggg gttcaagctc
     9001 gcgctgatga cgaaggacgt tgcaatcgcc ggggacctcg ctaaaacgct aagagtgaac
     9061 acgccctatt tgagggaaac cttgaagcac tggcgcgcag cgcaaaagca gctgccgcgc
     9121 aatgcggatc acaccgagat ctacaagtat cagaaaaggg ttctgagctc tgctaagcgc
     9181 cgatgaagcg gggcttgcgc ttctccatga aggcggcctg gccttctttg tagtcctggc
     9241 tcttgaagca ggcttcgacc gcgcgctcgg tggcgccgac gtcgcggttc tcggggtcct
     9301 tgaggacttc gccgacgacg agcttggcgc acttaacggt gagcggcgcg ttctccacga
     9361 tggtcctcgc catctcgccc acgacggtct cgagctgctc gtcggcgacg acgcggttca
     9421 cgaggcccat cttctcggct tcctgcgcgg tgaactggcg gccggtgaag aacatctcct
     9481 tggcgtaggc cgggccgatc aggtcgacga gcggcttcgc gcggttcatg ccgtagccca
     9541 ggccgagctt ggcggcggga acgccgaatt tggcgttttc ggtgcagatg cggatgtcgc
     9601 agcacaccgc gagcgcggtg ccgccgccga tgcaatagcc gcggatcatc gcgatggtcg
     9661 ggatcggcat gccgagcacc gtgtcgtaag ccttctgcgt ggtcgcctgg taatgcgcca
     9721 ccgcggccgc ttcctgacgc tcatccttga acttggaaat atcggcgccc gaagcgaagg
     9781 ccttgccgcc ggcaccggtg acgaccacga cgcgtacgga cttgtcggcg cgaaagtcgt
     9841 ccatcgcctc catggccgcg gtccacattt cgagtgagat ggcattgtgg cgctcggggt
     9901 tgttgaggac gatccagccg acaccgttct ctttcttggc gatgagcttt tcgctgggca
     9961 tcagacgatt cctttcttgc gcaattcctc gatcttgtcc ttggaaaaac ccacttcctg
    10021 caaaacctgg tcggtgtgct cgccctgctt gggcggatgc gcggccacct tgctcggtgt
    10081 gcgcgacagc gtcaccggct ggcccaggta tggcacctgg ccgacgttct gcaccatgcc
    10141 gaggctcttc acctgcgggt cgttgaacgc ctggtcgacg gagtagatgg gcccggccgg
    10201 gaccccggcg tcattgagcg tttcgatcca gtacgcagtg tccttcttta cgaaatactc
    10261 ctcgaggacc tcgtgcagcg cgtcgcggtt ctgtgatctc aagcttgaac tggaatactg
    10321 aggatgcgtc gcgagctccg gcacgccgat cgcctcgcac atgcgcgtcc agatcttggt
    10381 gccggtcacg gcgagattca tgtagccgtc gcgcgtcttg tagacgccgg tcgggatgga
    10441 agtcggatgg ttgttgcccg cctgctttgc gacttcgccc ttcatgagcc agcgcgaggc
    10501 ctgaaagtcg agcatgaaaa gctgcgcctg aaggagcgac gatttgaccc actggccttt
    10561 gcccgacttt tcgcgttcga tcagtgcgac ggcaatgccg tacgcgcaga agatgccagc
    10621 gcagaggtcc gcgatcggga tgccgacgcg caccggtccc tgccccggca gtccggtaat
    10681 cgacatg
//
LOCUS       DASTPD010000271         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_138698, whole genome shotgun sequence.
ACCESSION   DASTPD010000271 DASTPD010000000
VERSION     DASTPD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138698"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFR57_08965"
     CDS             <1..230
                     /locus_tag="VFR57_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259532.1"
                     /translation="MPLQMGDKQSHFVLTAMPQTCAFCLPGGPESMVEVKSKTPVKYT
                     FEPVVLTGKLSVLKDDPTGVFYRLTDAVPAK"
     gene            231..539
                     /locus_tag="VFR57_08970"
     CDS             231..539
                     /locus_tag="VFR57_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259533.1"
                     /translation="MLARLARVFLAAALLAAWQSALQHPVEHVDELGDFVHLAGGHSD
                     EGGEPLCDLLAALTACAAQGPLFLGTTASQYDAPALARRTPRAADAPPFLAQGPPALA
                     "
     gene            583..831
                     /locus_tag="VFR57_08975"
     CDS             583..831
                     /locus_tag="VFR57_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259534.1"
                     /translation="MKLVYWIVAAAFVLSGAAFAQEKHGSKEEQEDAKRHRTMAQAHE
                     AAAKCLEAGKGEKACLEQLRKDCKGVGIGKYCGMKHQH"
     gene            837..1103
                     /locus_tag="VFR57_08980"
     CDS             837..1103
                     /locus_tag="VFR57_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU0259535.1"
                     /translation="MAHYRKALAGAVAVNTAIVGVELAAGWEAGSLSLVVDGVHNLSD
                     ELALLALYLAFVLPQGVSRALLRSGNLFNSLYRGALSSTRSSRP"
     gene            1276..1812
                     /gene="hslV"
                     /locus_tag="VFR57_08985"
     CDS             1276..1812
                     /gene="hslV"
                     /locus_tag="VFR57_08985"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU0259536.1"
                     /translation="MEQFHGTTILSVRRGASVALGGDGQVTLGQVVLKGGARKVRRLY
                     QDRILAGFAGGTADAFTLFERFEAKLDKHQGNLMRAAVELAKDWRTDRVLRRLEAMLA
                     VADKERSLIITGAGDVLEPEYGILAIGSGGPYAQAAARALLENSSLTPKEIVDKALAI
                     AGDICIYTNQQRTIESLE"
     gene            1809..3125
                     /gene="hslU"
                     /locus_tag="VFR57_08990"
     CDS             1809..3125
                     /gene="hslU"
                     /locus_tag="VFR57_08990"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0259537.1"
                     /translation="MSGLISVMTPQEIVHELDKYIVGQGKAKRAVAIALRNRWRRQQV
                     AEPMRTEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDT
                     IVRDLVEISVKQTREQAMRRVRTRAQDAAEERILDVLVGFKQNEESAARQAFRKKLRE
                     GELDEREIDIEVAASQPHMEILAPPGMEELTSQIQGMLSGLGGGRRRLRRMKIREAAQ
                     IITEEEAARLVNDDELKVRAVANAEQNGIVFLDEIDKITSRGEVAGADVSRQGVQRDL
                     LPLVEGTTVSTRYGMVKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELDSLSVDD
                     FERILVATDASQVKQYQALLGTEGVALEFRPEAIRRIAELAFQVNERTENIGARRLYT
                     VLEKLLEEVSFEAGKRGAGTVTIDATYVDARLADLARNEDLARFVL"
BASE COUNT          595 a         1090 c         1109 g          553 t
ORIGIN      
        1 tcatgccgct gcagatgggc gacaagcaga gccatttcgt cctcaccgcg atgccgcaga
       61 cgtgcgcgtt ctgcctgccg ggcggcccgg agagcatggt cgaagtgaag agcaagaccc
      121 cggtgaagta caccttcgag cccgtggttc tgaccggcaa gctgtccgtg ctgaaagacg
      181 atcctaccgg cgtcttctac cgcctcaccg acgccgtccc cgcgaagtag gtgctcgccc
      241 gtctcgcacg ggtcttcctc gcagcagcgc tgctcgccgc ctggcagtcg gcgctgcagc
      301 atccggtcga gcacgtcgac gagctcggcg atttcgtcca cctggccggc ggccattcgg
      361 atgaaggcgg agagccgctg tgcgacttgc tcgctgcgct caccgcttgc gcggcgcagg
      421 gcccgctctt ccttgggacc acggcgtccc agtacgacgc acccgcattg gcgcggcgga
      481 cgccgcgcgc tgcggacgcg ccgccttttc tcgcgcaagg ccctcctgcc ctcgcctgac
      541 gctcggcaac ggtagtttcc ggacaatgca tcaggaggcg acatgaagct tgtgtattgg
      601 atcgtggcgg cagcattcgt gctgtccggc gcggcattcg cgcaggaaaa acacggcagc
      661 aaggaagagc aggaggacgc gaagcgccat cgcaccatgg cgcaggcgca cgaggcagcg
      721 gcgaaatgcc tcgaggccgg caaaggcgag aaggcgtgcc tcgagcagct gcgcaaggac
      781 tgcaagggcg tcggcatcgg caagtactgc gggatgaagc accagcacta ggcgccgtgg
      841 cgcactatcg gaaggcgctc gctggtgcag tcgcagtcaa tacggcgatc gtaggcgtcg
      901 agctcgctgc cggctgggaa gccggcagcc tcagccttgt cgtggacggg gtgcacaacc
      961 tgtccgacga gctggcgctg cttgccctct acctcgcatt cgtgctgccg cagggcgtgt
     1021 cgcgcgcgtt gctgcggtcc ggcaatctct tcaactcgct ctatcgaggc gctctttctt
     1081 cgacccgttc gtcgcggccg tgattgcagc ctggatcgtc ttttccaccg cgcgcgaggt
     1141 gctcgcctcg tgcgcgacga gctcatctgg cccgaaaagc tcgtctgcgg ccatcctgag
     1201 cgcgacgcgc aatagaggga ttgcaattcg gggccggcgg cctcatcttt gcttttctcg
     1261 gcttttccac ttcccatgga acagttccac ggcaccacca ttctctctgt tcgccgcggc
     1321 gcgagcgttg cgctcggcgg cgacggccag gtgacgctcg ggcaggtcgt gctcaagggc
     1381 ggcgcgcgca aggtgcggcg cctctaccag gatcgcatcc tcgccggctt tgccggcgga
     1441 acagccgacg cattcacgct gttcgagcgc ttcgaggcga agctcgacaa gcaccagggc
     1501 aacctgatgc gtgcggcggt cgagctcgcg aaggactggc gcaccgaccg tgtgctgagg
     1561 cgcctggagg cgatgctcgc ggtcgccgac aaggagcgct cgctcatcat caccggcgcg
     1621 ggcgacgtgc tcgagcccga gtacgggatt ctcgccatcg gctccggtgg tccttatgcg
     1681 caggccgcgg cgcgagccct gctcgagaac tcttcgctga ccccgaagga aatcgtggat
     1741 aaagcgttgg cgatcgccgg cgacatctgc atctacacca atcagcaacg aaccatagaa
     1801 agcctggaat gagcggcctc atttccgtga tgacgccgca ggaaatcgtc cacgagctgg
     1861 acaagtacat cgtcggacag ggaaaagcca agcgcgccgt cgctatcgcc ctgcgcaacc
     1921 gctggcgccg ccagcaggtc gccgagccga tgcgcaccga gatcaccccc aagaacatcc
     1981 tcatgatcgg gcccacgggt gtcggcaaga ccgaaatcgc gcggcgcctc gcccggcttg
     2041 cggacgcgcc tttcatcaag gtcgaggcga ccaagttcac cgaggtcggt tacgtcgggc
     2101 gcgacgtcga caccatcgtt agggacctcg tcgagatcag cgtcaagcag acgcgcgagc
     2161 aggcgatgcg gcgggtgcgc acgcgcgcgc aggacgcggc cgaggaacgc atcctcgacg
     2221 tcttggtcgg cttcaagcag aacgaggaat ccgcggcgcg ccaagcgttc cgcaagaagc
     2281 tgcgcgaggg ggagctcgat gagcgcgaga tcgatatcga ggtcgcggcg agccagccgc
     2341 acatggagat ccttgcgccg ccaggcatgg aggagctcac ctcgcagatc caggggatgc
     2401 tctcgggcct gggcggggga agacggcggc tgcgccgcat gaagattcgg gaagcggcgc
     2461 agatcatcac cgaagaagag gcggcccgcc tggtgaacga cgacgagctg aaagtgcgcg
     2521 cggtcgccaa cgccgagcag aacggcatcg tgttcctcga cgagatcgac aagatcacga
     2581 gccgcggcga ggtcgcgggt gccgacgtct cgcgccaggg cgtgcagcgc gacctgcttc
     2641 cgcttgtcga aggcacgacg gtctcgacgc gctacggcat ggtgaagacg gaccacatcc
     2701 tcttcatcgc ttcgggagcc ttccatctcg ccaagccgtc ggatctcatt cccgagctgc
     2761 agggccgctt tccgatccgc gtcgagctcg attcgctctc ggtcgacgac ttcgagcgaa
     2821 tactggttgc gaccgacgca agccaggtga agcagtacca ggctctgctc ggcaccgaag
     2881 gcgtggcgct ggaattccgt cccgaggcaa tccgccgcat cgccgagctc gccttccagg
     2941 tcaacgaaag gaccgagaac atcggcgcgc ggcggcttta cacggtgctg gagaagctgc
     3001 tcgaggaggt atcgttcgaa gccggcaagc gcggtgccgg caccgtgacg atcgacgcga
     3061 cttacgtcga tgcgcgcctt gccgatctcg cccggaacga ggacctcgct aggttcgttc
     3121 tttaggcgat cctctcagtt cttcttccaa gcacttgcgg aacaggcacg cgcgcccggg
     3181 aatgggcagg agcgccggca ggctcgcgca ccagcattca ggctcgttcc tgccgcagtg
     3241 aaacggtgcc ccgcagcggg cgcacttctc agggtttggg ctgttctgca agccgcgaga
     3301 gggccttgcg caggaaaagc atctgcttct tgaagttggt gcagccg
//
LOCUS       DASTPD010000272         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_346657, whole genome shotgun sequence.
ACCESSION   DASTPD010000272 DASTPD010000000
VERSION     DASTPD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346657"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFR57_08995"
     CDS             complement(<1..391)
                     /locus_tag="VFR57_08995"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447749.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0259538.1"
                     /translation="MKPINVGLLGIGTVGGGTWDVLNRNADEIQRRAGRAIRIAVVAD
                     KDLDRAKKIVGQKAKIVDDAFGVVRGNEVEIVVELIGGTAVARDLVLEAIAHGKHVVT
                     ANKALLATHGNEIFAAAQKKGVMVAFEA"
     gene            complement(378..977)
                     /locus_tag="VFR57_09000"
     CDS             complement(378..977)
                     /locus_tag="VFR57_09000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0259539.1"
                     /translation="MIHPKPAMLEGHGVRLEPLSLDHEKPLMEAAKDGRLWELFFTSV
                     PETHNTKKYIEEALKGQERGDMLPWAVRDLKSGAIVGSTRYHDIIAAADRVEIGWTWY
                     AQRVQRSHVNTACKLLLFDYAFESLKCKVVGLRTDNFNFRSQRAIEALGAKKDGVIRH
                     HWPRRDGTIRDSVMYSVLVHEWPDVKRHLEFRLKRNETH"
     gene            complement(1003..>2062)
                     /gene="alaC"
                     /locus_tag="VFR57_09005"
     CDS             complement(1003..>2062)
                     /gene="alaC"
                     /locus_tag="VFR57_09005"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEU0259540.1"
                     /translation="KLVEASQRIDTHGYSVSRGIPRLRKAIADWYRRRYAVDLDPDAE
                     AIVTIGSKEGLAHLMLACLERGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTPGVDFF
                     AELERAIRELMPKPKMLVIGFPSNPTAQCVDLSFFEKIIALARQHDILVVHDLAYADI
                     VFDGGWVNNCAPSIMQVPGAREVAVEFFTMSKSYNMAGWRIGFMVGNRDLVSALARIK
                     SYHDYGSFTPIQVASIVALEGPQDCVEEIRTVYEKRRNVMVKGLHDIGWRVDVPRASM
                     YLWAKIPEPYAGLGSIEFTKRLLEGAHIAVAPGIGFGDYGDDHVRIALIENEHRLRQA
                     VRGIRDMFRKDGLLRGAA"
BASE COUNT          332 a          683 c          635 g          412 t
ORIGIN      
        1 cggcctcgaa cgccaccatc acgcccttct tctgcgcggc ggcgaaaatc tcgttgccgt
       61 gcgtggcgag cagcgccttg ttcgcggtga ccacatgctt gccgtgggcg atcgcctcga
      121 gcacgaggtc tcttgccacg gcggtgccgc cgatcagctc gaccacgatc tcgacctcgt
      181 tgccgcgcac cacgccgaaa gcgtcgtcaa caatctttgc tttttgccca acgattttct
      241 ttgcgcgatc aaggtctttg tccgctacga ccgcgatgcg gatcgccctt ccggcgcggc
      301 gctggatttc gtcggcgttg cgattgagca cgtcccaggt gccgccgccc accgtaccga
      361 tgccgagcag gcctacgtta atgggtttca tttctcttca accggaattc gagatgccgc
      421 ttcacatccg gccactcgtg cacgagcacg ctgtacatga cgctgtcccg gatcgtgcca
      481 tcccggcgcg gccagtggtg gcggatgacg ccgtccttct tcgcgccgag cgcttcaatc
      541 gctcgctgcg agcggaagtt gaagttgtcg gtacgcagtc ccacgacctt gcactttagc
      601 gactcgaacg cgtagtcgaa cagcaacagc ttgcatgccg tgttgacgtg gctgcgctgc
      661 acgcgctgcg cataccaggt ccagccgatc tccacgcggt cggccgcggc gatgatgtcg
      721 tggtaacgcg tcgagccgac gattgcgccg ctcttcagat cgcgcaccgc ccagggcagc
      781 atgtcgcccc gctcctgccc cttgagcgcc tcctcaatgt acttcttcgt gttatgggtc
      841 tcgggcacgc tggtgaagaa cagctcccac agccggccgt ccttggcggc ctccatcagg
      901 ggcttttcgt gatccagaga gagcggttcg aggcgcaccc cgtgcccttc gagcatagcg
      961 ggcttggggt ggatcacgcc gcgcctgctt tttgcggcgc catcacgccg cccctctgag
     1021 cagtccgtcc tttctgaaca tgtcgcggat gccgcgcacc gcctggcgca ggcggtgctc
     1081 gttttcgatg agcgcgatgc gcacgtgatc gtcgccgtag tcgccgaagc cgatgccggg
     1141 cgcgaccgcg atgtgcgcgc cctcgagcag gcgcttggtg aactcgatcg agccgagccc
     1201 ggcgtagggc tcggggatct tcgcccagag gtacatcgag gccctcggca cgtcgaccct
     1261 ccaaccgatg tcgtgcaggc ccttcaccat gacattgcgt cttttctcgt aaaccgtcct
     1321 tatttcctcg acgcagtcct ggggcccttc gagcgcgacg atcgatgcca cctggatcgg
     1381 cgtgaagctg ccgtagtcgt ggtagctctt gatgcgcgcg agcgcgctca cgaggtcgcg
     1441 gttgccgacc atgaagccga tgcgccagcc ggccatgttg tagctcttcg acatggtgaa
     1501 gaactcgacc gccacctcgc gcgcgcccgg cacttgcatg atggaaggcg cgcaattgtt
     1561 aacccagccg ccgtcgaaca cgatgtcggc ataggccaag tcgtgcacga cgaggatgtc
     1621 gtgctgcctc gcgagcgcaa tgatcttctc aaagaaactc agatcgacac attgcgcggt
     1681 cggattcgac gggaacccga tcaccagcat cttcggcttc ggcatgagct cgcggatcgc
     1741 gcgctcgagc tcggcgaaga aatccacgcc cggcgtcatg cgcaccgagc ggatgtcggc
     1801 gccggcgatg attgcgccgt agatatggat ggggtacgac gggttgggca cgagcaccgt
     1861 gtcgccgcgc tcgaggcacg cgagcatgag atgcgcgagt ccttccttcg agccgatggt
     1921 gacgatcgcc tcggcgtcgg ggtccaggtc aaccgcgtag cggcgccgat accagtctgc
     1981 gatcgctttt ctcagcctcg ggatgccgcg cgagaccgag tagccatgcg tgtcgattcg
     2041 ttgcgacgcc tcgaccagct tt
//
LOCUS       DASTPD010000273        22957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1776974, whole genome shotgun sequence.
ACCESSION   DASTPD010000273 DASTPD010000000
VERSION     DASTPD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776974"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFR57_09010"
     CDS             complement(<1..388)
                     /locus_tag="VFR57_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0259541.1"
                     /translation="MRVLVLSKRQYMGKDLLDDAYGRFYELPLELAERGHEVRAVCAS
                     YQPRREGVHDRRRAGAQVSWLSKNVSPIAPWSLPGWTRALEREARHFRPDVIWACSDS
                     FHAIVGVGIQRRLGTPCVVDLYDNFES"
     gene            complement(394..1602)
                     /locus_tag="VFR57_09015"
     CDS             complement(394..1602)
                     /locus_tag="VFR57_09015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0259542.1"
                     /translation="MRLRILVLTSTFPRWPADNEPPFVFELCRRLAAAHEVLVIAPHC
                     RGSATEERLGANLTVRRFRYAPERLQSLAYEGGILERLRQRRWRFLLVPFFIGAELIA
                     ALRAVRREKPDVIHAHWIVPQGLVGLLAALAAPRSKRPALVCTSHGADLFALRGLLAM
                     RLKGCVVRRAAALTVVSTAMMAVALRLGVRAEKVRVMPMGIDARQRFAPAHGAERAPN
                     ELLFVGRLVAKKGVGHLLDAFGLVRESVADARLTIVGAGPLEAELRASARAHGLEAHV
                     SFAGAVLNEDLPAYYRRVAAFVVPSVVAPDGDQEGLGLVIGEALACECPVVASDLPAI
                     RDLIQDGVTGLLARPGDARDLADKLLRLLRNPAAAAGFARRGRGLILERFDWQNVGQR
                     YEELFAAVAG"
     gene            complement(1609..2484)
                     /locus_tag="VFR57_09020"
     CDS             complement(1609..2484)
                     /locus_tag="VFR57_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0259543.1"
                     /translation="MSGPSLSVILPAKNEATNLQSLLPRLKAMAAGLKRDVEIIVVDD
                     GSTDETQVVCAALGVRVLRHPYSSGNGAAIKTGVRAARGQVLAFMDADGQHDPELIPR
                     LLEKLDEGYDMIVGARTAEAHAGAHRLAANTFYNWFASWMIGRPVPDLTSGLRVARAE
                     RFRRFLYLLPNGFSYPTTSTMSFFRCGYPVAYVPVPVQRRVGRSHIRLLRDGLRFLLI
                     IFKIGTLYSPLKLFVPISAAFFLTGLGYYVYTYVTVNRFTNMSALLFVTAVLVFVVGL
                     LSEQITLLNFKDSDK"
     gene            complement(2481..4439)
                     /locus_tag="VFR57_09025"
     CDS             complement(2481..4439)
                     /locus_tag="VFR57_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259544.1"
                     /translation="MSEPRRKAGFFFVHSQLRFEYARVALLAVLAGAAVLFSFGLPGG
                     FLLDDFGNLSNLGQVSEGAIYGAIYLTEGVGSPGRPISYLSFLLQSGSWPHDPQAFKV
                     VNIALHLANGALVYAVAARMLAMMGRSDAAPLALLVAGVWLIHPIQLSTVLYVVQRMT
                     ELAALLCLCAILAYLKGRAFASAGRIKHGYAWMTSAVVLGTLLAVLAKENGALLPVYI
                     AVIELTLMAGLARPARWNLWAAAFLALPPLALASYIVLSPTWLSYGGRDFSMSERVYT
                     EAIILWDYVSKIALPRPRAFGIYFDDYPVAAAPWASLATAAALVGWCVALGFAMVWRR
                     RQPFLSFATLWFLGGHLLESTVIPLELYFEHRNYLPLLGPVLAAAWVAKRFWDTASTP
                     ATRYAYASLGLAGAISFAGITWVEARTWAHPVQQVVVWASERPTSQRAQYALGVQYLY
                     AGRYAEASAAWERAHASAPQESYFILARMLLGCMAHNVPVPETREVVLRIANGALRPV
                     DVELLDNLARQLERGTCRRIPIDDALAVADAFLSNHKAADGPHKWTALYVKGRLYALK
                     RDLDPAIRALEAADEIRPNLGVLNLQVSWLASAQLFDEALDFIEKGRHDRRWKPWQRV
                     LYAGFFDAWEQQVRALAHREQAAARSEQ"
     gene            complement(4454..4891)
                     /locus_tag="VFR57_09030"
     CDS             complement(4454..4891)
                     /locus_tag="VFR57_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU0259545.1"
                     /translation="MKQLQKGFTLIELMIVVAIIGILAAVAIPAYQDYIARSQVGEAV
                     NLLAGGKTPLAEFYADKGQWPNFATSVMGNTTGKYTSTIAISFGDGSTAQNLVLTATM
                     KAAGSVNKAIAGGTVTLETNDGGKIWTCKTGTLQAKYVPGACK"
     gene            complement(5044..5472)
                     /locus_tag="VFR57_09035"
     CDS             complement(5044..5472)
                     /locus_tag="VFR57_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU0259546.1"
                     /translation="MRKNREGFTLIELMIVVAIVGILASVALPAYQDYLARSQVSEAV
                     TLLSGAKNPLSEYIYDKGAPPDAATFNELVPVRQGKYVESMVLTGTISNLVLTATMKP
                     TEVSQPIQSRTVTLSTTDMGKSWTCAPGTVGARFLPSACR"
     gene            complement(5543..8515)
                     /locus_tag="VFR57_09040"
     CDS             complement(5543..8515)
                     /locus_tag="VFR57_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259547.1"
                     /translation="MSGARAFLPLALAVFTASGFAGLMYESVWTHYLKLFLGHSAYAQ
                     PLVLGIFMGGMALGAWAASRLSQRLRDLLLAYALIELVVGIAATVFHPLFVAATGAAF
                     DHVIPALGSPAAAHAFKWALGAALVLPQSVLLGMTFPLMTGGVLRLGPARPGYAVAML
                     YFTNSLGAAVGVLASGFYFISAVGLPGTLVAAGVINLAVAGAVMLLPGRKAALGREPT
                     PTTARISVADLRLLLAVAALTGMSSFMYEIGWIRMLSLVLGSSTHAFELMLSAFILGI
                     AFGGLWVRRRIDAAGSTVRFLAWVQLAMGVAALATIPVYGWTFHAMARTLDALARTES
                     GYLVFNVVSHALCLAVMFPAAFCAGMTLPLITVSLLRQGAGERAIGQVYAANTAGAIV
                     GVLLAVHVGFTVLGLEGLIAAGAAIDLALGVVLLRAAAPAGFRRVPLVAAGVAAAALG
                     AALLVPLDARLMASGVYRMGGLLPEHHALHRQLHGKTATVSITESRGTYSVRTNGKPD
                     GALRLDRGEPAPDEVTMTILGALPLVLAPEARRVANIGFGTGMTTHVLLASPQLERVD
                     SIEIEPAMVEAVSAFRRANGRAYEDPRSHIHYEDAKTFFSVRRERYDVIVSEPSNPWV
                     SGVASLFSAEFYRDVRRHLKDGGLLLQWVQLYEITPELLATIVTALHANFSDYEFWLP
                     GSADLVIVAAHNGSVPAPDARALAHPALRAELERFSIASPEDLALHRVAGRAALAPYF
                     ANFRVPPNSDYFPALAMKAPLARYMQSAAPDFAMLSHAPLPLLPLFDERARRQPEAQR
                     LSSGERPWLPRAASVREAHAAQHFLASGNARFLTGVEAQPAASLVLLRAALVECRVRL
                     PEGVLPHELAALARLVGAQLPPPAAVSLWTGLASTPCGAHLSEADRRWLRLHAAVAAA
                     RPAEMARAAQAILEAESNLTPELVAHALAASMAASILSREGEAALAAFMRHRDVFTRM
                     APEWRPVFRFLIAHAERM"
     gene            complement(8512..10140)
                     /locus_tag="VFR57_09045"
     CDS             complement(8512..10140)
                     /locus_tag="VFR57_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU0259548.1"
                     /translation="MKPADNTHGLRLRAAANRDADLGERLEKLNLIGASLSAERDINR
                     LLELILAAAKSITGADGGTLYRVADDKTLRFEIVRTTSLRYYLGGTSGNPVPFYPIQL
                     YRDGEPNHSMVAAHAALTGRTVNIADAYGAEGFDFSGTRAFDAKTGYRSKSFLTVPMR
                     NHEHETIGVLQLINAADPRSGEIVAFSASDQRLAESLASQAAIVLSNRQLINQLEQLF
                     ESFINLINSAIDEKSPYTGAHCQRVPQLTMMLAEAVNDTHEGPLAKFQMTDKDRYELK
                     IAGLLHDCGKVTTPVHVVDKATKLETIYDRITLIDTRFEVLKRDLEIQALRGELDERK
                     LRVRLRELDDDRRFLHACNMGSERMKDEDVARVKRIAAYRWRDVAGHEANFLSDDEVK
                     NLTIRAGTLTEEERKIINHHIVATIKMLEALPWPKHLANVPEYAGGHHERMDGRGYPR
                     GLKREQMSVQARCMGIADIFEALTARDRPYKKGKTLSESLEILGRMKLNHHVDPDLFD
                     IFVRKKVYRRYAEMFLEPEQIDEVDESRIPGYQP"
     gene            complement(10159..12528)
                     /locus_tag="VFR57_09050"
     CDS             complement(10159..12528)
                     /locus_tag="VFR57_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0259549.1"
                     /translation="MSAVLNHAPAHTDVGARLAFQKQLQAVTNKIHATSNIDEIMLEV
                     SADICQLFHADRLTIYSVGEDKQSIVSKVKTGLNSFKDLKLPIAEHSIAGYVALAKRT
                     ANVRDVYDENELRGLNPNLRFLQEVDKRTGYRTKQMLVAPILEAGTNELIGVIQIINN
                     KAGVPFGGLAEEGVSELAQTLAIAFKQRQKSQIAKSKYDYLIADAVISLGEFELAARK
                     ARKKAVDIEQILVDEFQVNNAAIGAALSKFFGVPYEPFKPDRVKPGDLLKNLKREYVE
                     ANQWVPIDDSRQGLAVLCLDPERIGSSRIAANVFPKARIIYKVTTQKDFKDTVNQFYG
                     PESGAADLGNVEDLLGAMADDAGDILEASNDEASAAADNELVKLVNKVIVDAYNQGAS
                     DIHIEPYPGKAKTEIRFRKDGSLGPYIEVPASYRAAIVARIKIMCDLDISEKRKPQDG
                     KIKFKKFGPLDIELRVATIPTAGGIEDIVMRILAAGEPIPLDKMGFSERNLQHLKTAV
                     SKPYGLFFCCGPTGSGKTTTLHSVLKFLNTPDTKIWTAEDPVEITQKGLRQVQVNKKA
                     GLDFATVMKAFLRADPDIIMVGEMRDKETTGIGIEASLTGHLVFATLHTNSAPESITR
                     LLDMGMDPFNFSDALLGILAQRLAKRLCGCKQTYTPEAAELTSFLREYCEELMNTPRF
                     KADPKGAMEAVYREWVKVYGNDKGQLTFYKPVGCDRCGGSGFKGRCGLHELLIASDRL
                     KKAIQEHARVAEVLAIALEEGMRTLKQDGMEKCLAGITHIKEVRAVCIK"
     gene            complement(12608..13696)
                     /locus_tag="VFR57_09055"
     CDS             complement(12608..13696)
                     /locus_tag="VFR57_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0259550.1"
                     /translation="MRRRVPAAHAAKQPRPGILNASPDQLAALAPLAGLSRERLAELS
                     EVAAIERAPRGADPFSGRTLAKQSVFLLAGELLLSFQAGGTLVVVGGSEETRHALNRR
                     KETVARARAITDVDLLVLEDDLLDILATWDQVAASRGREMAASGAFSLSNLRTIFAPL
                     PPARIDELFRRFERRPCARGEVVIREGDEGDYYYVIESGRFQVRRTVGGAEVVLAELK
                     TGDAFGEEALVSEAKRNATVTALTDARLLRLDRRHFNELLREPLLRRLGYAEALDKVR
                     RGALWLDVRYPSEYQYDKLAGAINVPLAEVRNMFAVLDRSKEYIAYCQSGRRSAAAAF
                     LFAQRGFKVWLLEDGLKKSRESGQNARS"
     gene            complement(13639..14358)
                     /locus_tag="VFR57_09060"
     CDS             complement(13639..14358)
                     /locus_tag="VFR57_09060"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEU0259551.1"
                     /translation="MRTTSFLLDSDILIDAGSGVGDLTLAELSQIDHIFVTHSHLDHV
                     TSIPFLVDTVGGMRSKPITLYATRATIEILKNHLFNWAIWPDFSEIPSAEAPFMRYSE
                     IEVGATITLAGRSITPIPARHTVPAVGYHLDSGAASLVFTGDTGPNDALWRIVNRIAN
                     LKFLIIETAFSNKERQLAEISRHLCPATLAEELAKLERNAEIYITHLKPGEIEPTMLE
                     IEECAGAFRPRMLQNNLVLEF"
     gene            complement(14867..15346)
                     /locus_tag="VFR57_09065"
     CDS             complement(14867..15346)
                     /locus_tag="VFR57_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0259552.1"
                     /translation="MPGGRDERIAILDIDERSLAEVGRWPWSRRFVRIPRRVTPEAPA
                     RRALPRIRPRQKCSHGARSRTLRQAQERRAHDLFADIRGFTGISESLSPEALRDYIDE
                     YLTAMSAVIRERHGGTLDKYIGDAIMAFWGAPIDDADHALNSRLARCGCRVWPSASA"
     gene            complement(15486..16289)
                     /locus_tag="VFR57_09070"
     CDS             complement(15486..16289)
                     /locus_tag="VFR57_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0259553.1"
                     /translation="MAKLILSMDGLVLKEIPLTRERTSIGRKPHNDIQIDNLAVSGEH
                     AVIVTILNDSFLEDLGSTNGTVVNGNPIKKHFLQNNDVIELGKYKLKFVGEAAPAAAA
                     DKADFEKTMVLRPAAVRAAAEVKAPAANPGGGVAAAAVALRAAAPVSASAPQPAEKDA
                     VKPAPPPPAAAPAAPARPAQPLGAIQLLSGANAGRELELTKPLTTLGKPGVQVAVLTR
                     RPQGYFITHVEGANRPIVNGQAIGAAPHALKDHDVIELAGVKMEFFLKV"
     gene            complement(16306..17130)
                     /locus_tag="VFR57_09075"
     CDS             complement(16306..17130)
                     /locus_tag="VFR57_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU0259554.1"
                     /translation="MDLSQSLEIASCTDPGMVRSHNEDSIAADPANGLVVLADGMGGY
                     NAGEVASGMATTVILSEMRQILQSAKPYDIDSRTNQQLAARLVREQVLKANSSIYHAA
                     QSQPQYAGMGTTLVVCLFYDNRVLVAHLGDSRVYMLREGRFSQVTRDHSLLQEQIDSG
                     IITPEQAKKAQHKNLVTKALGIDPTVEPEIREYSTKTGDIYLLCSDGLCDMVDDEDIG
                     MTVQTLGGNLKLAAQQLVQMANDHGGRDNVSVILVRVLREYPGPRGVMAKVFGWLK"
     gene            complement(17194..18123)
                     /locus_tag="VFR57_09080"
     CDS             complement(17194..18123)
                     /locus_tag="VFR57_09080"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0259555.1"
                     /translation="MKIAIIGQQDFGKAVLEAFLARKDEVAGVFCAPEKPGARPDALK
                     VAAQEKGVKVFQFPTLKSEEAKKALKDLNVELGVMAFVLQFAPQDFVRIPKRGTIQYH
                     PSLLPKYRGPSSINWPIIRGDARTGLTIFRPTDGLDEGPVILQKETAIGPDDTLGTVY
                     FDRLFPMGVKAMLEAADLVVAGKHKEAVQDESQATYEGWCRRQEARINWANHVDFVYN
                     VIRGCNPAPGAWCTLEGKELQIYDARKHPVRTFGAVKGKIGEIVEVTDNSFQVTAQGG
                     RIEVMRAKLADGKKVAATELLAAGALRAGQILS"
     gene            complement(18120..19391)
                     /gene="frc"
                     /locus_tag="VFR57_09085"
     CDS             complement(18120..19391)
                     /gene="frc"
                     /locus_tag="VFR57_09085"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEU0259556.1"
                     /translation="MEALKGVRILDMTHVQAGPTCSQLLAWLGADVIKFENPQGDATR
                     GQLRDVPNADSLYFTMLNCNKRSITVNMKTSEGKQVFVDLVKKCDIIMENFGPGVLDR
                     FGFPWEKIKEINPKIVMGSIKGFGSTGPYAEFKAYENVAQAMGGAMSTTGVPDGPPFV
                     TGAQIGDSGTGLHLAIGLLAALRQAEKTGKGQYVEVAMMDGVMNLCRVKFRDHQRLQR
                     QELAEYSVPTYKGMGEVPRAGNDSGGGQLGNAIHCKPHGANDWLYVVVQEAVWEDLAK
                     RIGPEVGMSDMASDARFAKIGERRRNQSLMWTLINKFAEKYTKRELMAILNPLDVPCG
                     PIMSTEDLANDEHVRGRDMWVELDHPQRGKWFNVGMPIKLSDSPARIKRSPTLGEHTD
                     EILREVLGYDEGKIKNLRGAGAFSVPPKKAA"
     gene            complement(19391..21127)
                     /gene="oxc"
                     /locus_tag="VFR57_09090"
     CDS             complement(19391..21127)
                     /gene="oxc"
                     /locus_tag="VFR57_09090"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEU0259557.1"
                     /translation="MGTAEPQVAVEASQSTDGFQLVIDALKLNGIDTIYALPGIPITD
                     LTRRAQADGMRVLSFRHEQNAGYAASIAGFLTQKPGICLTVSAPGFLNGLNALAHATV
                     NCFPMILISGSSEREIVDLQQGDYEEMDQLAIARPVAKAAYRVLHADDIGVGVARAIR
                     AALSGRPGGVYLDLPAKLFAQAIDAATAKKTLIKVVDPAPKQIPAPEAVKRALDLLKS
                     AKKPLIILGKGAAYAQADAEIKALVERTGIPYLPMSMAKGLLPDTHEQCASAARSFVL
                     PGADVVMLIGARLNWLLSHGKGKTWGGKAPKDRGGHKFIQIDISPQEADSNVAIDAPV
                     VGDIGSCVSALLAGLGNWAKPPAEWLNAVAEKKNANVAKMAETLAKNPTPMNFHSALN
                     VVRDIVKANPEAILVNEGANALDFTRSIVDMYKPRKRIDVGTWGIMGVGMGYCVAAAV
                     TTKQPVIAVEGDSAFGFSGMEVETICRYNLPVCVVVMNNNGVYKGTDVNPRGGDVAPT
                     VFVKDARYEKLMEAFGGVGVYAKTPGELRKAMEEALRSKKPTLINAIIDETAGTESGR
                     ITSLNPTAGKKK"
     gene            21220..21846
                     /locus_tag="VFR57_09095"
     CDS             21220..21846
                     /locus_tag="VFR57_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0259558.1"
                     /translation="MTSLASHPQRNSLRLQLRNNALLQGLSPAQWAELEPLLVVADYR
                     KGDPLVRQGDEEMQQLFILEGMLKRVVSNPQGREMILRFAAEGEMDTSYAAWRLRTPV
                     PYSIVAVTKVRTVELAMPLWVEFLEKNTDVKSRFEYEVMKLMSEVMAHTITLHLLDAP
                     GRLARFQRKHPELAGRIPKKELAAYLNLTPETLSRLKHSNRGRVRPGL"
     gene            complement(21841..>22957)
                     /locus_tag="VFR57_09100"
     CDS             complement(21841..>22957)
                     /locus_tag="VFR57_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0259559.1"
                     /translation="VITFLSPVVAGFALEFGPPEFFAVYLLTFCAFVGTSKEPPFKTI
                     AAMMLGFALAAVGTDTVTGTLRLTFGSTHLLTGFDFLVAVIGLFGIGEILLTLEEGLA
                     FRGASAKINVRVVLDTWKELPRYWMTSLRSSAVGCFMGIVPGGATPASFMSYGVARRF
                     SKDGDKFGTGKVEGVVAPETAAHAAGTAALLPMITLGVPGSPTAAVLLGGLMIWGLQP
                     GPLLFVEQKDFVWGLIASMYLGNIAGLIVVLTCVPLFAAILRVPFSVIAPIIMVICAI
                     GSFTVKNAIFDVWMMVVFGILGYLFKKLQYPLAPLVLAIVLGDNAESSFRQAMLMSQG
                     DLSILFSKKLVGFMTTLALLLLFWPLLSLAIAKLRRS"
BASE COUNT         3977 a         7456 c         7495 g         4029 t
ORIGIN      
        1 agctttcgaa gttgtcgtac aggtccacca cgcacggcgt cccgaggcgg cgctgaatcc
       61 caacgccgac gatggcatgg aagctgtcgg agcatgccca tatgacgtcc ggtctgaagt
      121 gcctcgcctc gcgctcgagc gctcgggtcc accccggcaa cgaccacggc gcgatcggcg
      181 acacgttctt ggacaaccac gacacctgcg cgccggcgcg gcggcggtcg tgaacgcctt
      241 cgcggcgcgg ctggtagctc gcgcagacgg cgcgcacttc gtgcccgcgc tccgccagct
      301 ccagcggcag ctcgtagaac ctgccgtacg catcgtcgag gaggtctttt cccatgtact
      361 gccgcttgga gagcaccagg acgcgcacgc cggctatccc gcgaccgcgg caaagagctc
      421 ttcgtacctc tggccgacgt tttgccagtc gaagcgctcg aggatgagcc cgcggccgcg
      481 gcgcgcgaac cccgccgcgg cagcgggatt gcgcagcagg cgcagcagct tgtcggcaag
      541 atcgcgcgcg tcgccgggac gggcgagcag gcctgtgacg ccatcctgga tcagatcgcg
      601 gatcgccgga aggtcgctcg ccacgaccgg gcactcgcag gcgagcgcct cgccgatcac
      661 aaggccgagc ccctcctggt cgccgtcggg cgcgaccacg ctcggcacga cgaaagcggc
      721 aacgcggcgg tagtaggcgg gcaggtcctc gttgagtacc gcgccggcga agctcacatg
      781 ggcctccagc ccgtgcgcgc gggcgctcgc gcgcagctcc gcttcgagcg gtccggcgcc
      841 gacgatcgtc aggcgcgcgt cggcgaccga ttcgcgcacc aacccgaaag cgtccagcag
      901 atggccaacg cccttcttgg cgacgaggcg gccgacgaaa agcagctcgt ttggcgcgcg
      961 ctcggcgccg tgcgccgggg cgaagcgctg gcgcgcgtcg atgcccatcg gcatgacgcg
     1021 tactttctcg gctcgcacgc ccagacgcaa ggcgacggcc atcatggccg tgctgacgac
     1081 cgtgagcgcg gccgcgcgcc gcaccacgca gcccttgagc cgcatggcga gcagtccgcg
     1141 cagcgcgaag agatccgcgc cgtgcgaagt gcataccagc gctgggcgct tggagcgagg
     1201 tgcggcgagt gcggcgagca gaccgacgag gccttgcggc acgatccagt gcgcatggat
     1261 cacatcgggc ttttcgcgcc tgacggcgcg cagcgcggcg atgagctcgg cgccgatgaa
     1321 gaacggcaca agcaggaagc gccagcggcg ctggcgcagc ctctccagaa tcccgccttc
     1381 ataagcgagc gattggaggc gctccggcgc ataccggaac cggcgcacgg tcaggttcgc
     1441 gccaaggcgc tcctcggttg cggagccacg gcagtgcggc gcgatcacca gcacctcgtg
     1501 agccgcggcg aggcggcggc acagctcgaa tacgaacggc ggctcgttgt ccgcgggcca
     1561 gcgcggaaag gtcgaggtga gaacgaggat ccgcagccgg atggctccct acttgtcgct
     1621 gtccttgaag ttgagcaagg tgatttgctc tgatagcagc ccgaccacga atacaaggac
     1681 cgcggtgacg aaaagcagcg cgctcatgtt ggtaaagcgg ttcaccgtca cgtaggtgta
     1741 gacatagtag cccaggccag tgaggaagaa agccgcactg atcggcacga agagtttcag
     1801 cggcgaatac agcgttccga tcttgaagat gatgagcagg aagcgcaggc cgtcgcgcag
     1861 aagccggata tggctcctgc ccacgcggcg ctgcaccggg accggcacgt aggccaccgg
     1921 atagccgcag cggaagaaac tcatcgtgct ggtggtaggg taggagaatc cgttcggcaa
     1981 caggtaaaga aagcggcgga agcgctcggc gcgcgccaca cgcagcccgg acgtgagatc
     2041 gggcaccggg cgcccaatca tccagctggc gaaccagttg tagaacgtat tcgccgcgag
     2101 gcggtgcgcg cccgcatgcg cttcggcagt gcgggcgccg actatcatgt cgtagccctc
     2161 atcgagcttt tcaagcaagc gcggtatgag ctcggggtcg tgctgcccat cggcatccat
     2221 gaaggccagc acctggccgc gcgcggcacg cactccggtc tttatcgctg cgccgttgcc
     2281 agaagaatag ggatgccgca acacgcgcac cccgagcgcc gcacacacca cttgggtctc
     2341 gtcggtggag ccgtcgtcga cgacgataat ctcgacgtcg cgcttcaggc cggcggccat
     2401 tgcctttagt cgcggcagca gcgactgcaa attcgtcgcc tcgttcttcg cgggaaggat
     2461 gacggatagg ctggggccgc tcattgttcc gaccgagctg ccgcctgctc gcgatgcgcg
     2521 agcgcgcgca cctgctgctc ccaagcgtcg aagaaccccg cgtacaggac gcgctgccaa
     2581 ggcttccatc gccgatcatg ccgacccttc tcgatgaaat ccagggcttc gtcgaacagc
     2641 tgggctgacg cgagccagct cacctgcaaa ttgagcacgc cgagattcgg tcgtatctcg
     2701 tcggcggcct ccagcgcgcg gatcgccgga tcgaggtccc gttttagggc gtacaggcgg
     2761 cccttgacat acaaggcggt ccacttgtgc gggccgtcgg cggccttgtg gttggataga
     2821 aaggcgtccg ccaccgcgag ggcatcgtcg atcggaattc tccggcacgt gccgcgctca
     2881 agctgacggg ccaggttgtc aaggagttcc acgtcgacgg gacgcagtgc gccgtttgcg
     2941 atgcgcagaa cgacctcccg ggtttccggt accggcacgt tgtgcgccat gcagccgagc
     3001 agcatgcgcg ccagaatgaa atagctttcc tgcggcgcgc tcgcatgcgc gcgctcccag
     3061 gccgcgctcg cctccgcgta ccgccctgca taaaggtact ggacgccgag cgcgtattgc
     3121 gcgcgctggg aggtcggtcg ctcgctcgcc cagaccacca cttgctgcac cggatgagcc
     3181 caggtcctcg cctctaccca agtaatgccc gcgaaggaaa tcgcgccggc caggccgagg
     3241 gatgcatacg cgtaccgtgt ggcgggcgtg ctggccgtgt cccagaaccg ctttgctacc
     3301 cacgccgcgg ccagaaccgg cccgagcagc ggcagatagt tgcggtgctc gaagtacaac
     3361 tccagcggga ttacggtgct ttcgagaagg tggcccccaa gaaaccacag agttgcgaac
     3421 gacagaaatg gctgccttct gcgccatacc atcgcgaacc cgagggcgac gcaccagccc
     3481 accagggctg ccgcggtcgc caaggaggcc catggcgctg cggcgaccgg atagtcgtcg
     3541 aaatagatcc cgaacgcgcg cggccggggc agggcgattt tggagacgta gtcccaaagg
     3601 atgatcgctt ccgtataaac gcgctcggac atgctgaaat cgcgtccgcc atagctcagc
     3661 catgtcggcg atagcacgat gtaggacgcc aaagcgagcg gcggcaaagc gagaaacgca
     3721 gccgcccaga gattccagcg cgccggtcgc gccagcccag ccatcagcgt gagctcgatg
     3781 accgcgatat ataccggaag caacgccccg ttctccttcg ccagaaccgc gaggagggtg
     3841 ccaagcacga ccgccgatgt catccacgcg tagccgtgct tgattcggcc cgcgctggcg
     3901 aacgctcgac ccttgagata ggcgaggatg gcgcacaaac acaagagagc agcaagctcc
     3961 gtcatgcgct gcacgacgta gagcacggtc gagagctgga tgggatgaat gagccatact
     4021 ccagccacca gaagagcgag cggcgccgcg tccgagcgac ccatcatcgc gagcatcctc
     4081 gcagccacgg cgtaaaccag cgcaccattc gccagatgga gcgcaatgtt caccaccttg
     4141 aatgcttgag ggtcgtgggg ccagcttccg ctctgtagga gaaacgacag gtacgagatc
     4201 gggcgacctg gcgatcccac gccttcggtg agatagatcg cgccgtagat cgccccctcg
     4261 ctgacctggc cgaggttcga cagattgccg aaatcgtcga gcaggaaacc gccgggcagt
     4321 ccaaaggaga agaggaccgc ggcaccggcc agcacggcta gaagcgcgac tcgcgcgtac
     4381 tcgaagcgca gttgagaatg cacaaaaaag aaccccgcct tgcggcgggg ttcgctcaag
     4441 cggtcaggct cggttacttg caggcgcccg gaacgtactt ggcttgcagg gtaccggtct
     4501 tacatgtcca gatcttgccg ccgtcgttgg tctccagagt gaccgtgccg ccggcgatgg
     4561 ccttgttgac gctgcccgcg gctttcatcg tggcagtcag cacaaggttc tgagccgtcg
     4621 agccgtcacc gaagctgatc gcgatggtgg acgtgtactt gccggtcgtg ttccccatca
     4681 ccgaggtggc aaaattcggc cactggccct tgtcagcata gaactcggca agcggggtct
     4741 tgccaccggc cagaaggttc accgcctcgc ccacttgcga gcgcgcgatg tagtcctggt
     4801 acgccggaat cgccacggcg gcgagaatgc cgatgatcgc aacgacgatc atcagttcga
     4861 tcagcgtgaa gcccttctgc aactgtttca tttgtctcac tcccttaaag agccggagcc
     4921 ccggcttgca cctcacctgc aaactgcatg ccatcgggtt ggcggcccga agttacgaac
     4981 ttcgtcacgc tttccaccgt cccaccgacc aaaacaggca agtcgagtgc cgaaattcgt
     5041 cagttaacgg caagccgagg ggaggaaacg ggcgcccacc gtacccggtg cgcaagtcca
     5101 gcttttgccc atgtcggtcg tgctcagcgt caccgtccga ctttggatcg gctggctcac
     5161 ctcagtgggc ttcatcgtcg cggtgagcac aaggttgctg atggttcccg taagcaccat
     5221 gctctcgacg tatttccctt ggcgaaccgg gaccaattca ttgaacgtcg cagcgtcagg
     5281 cggagcaccc ttgtcgtaga tgtattccga aagcggattc tttgcgccgc tcagcagggt
     5341 cacggcctcg ctcacttgcg agcgagccaa gtagtcctga taggcgggaa gcgccaccga
     5401 ggcaagaatg ccgacgatcg cgacgacgat catcagctct atcagcgtga agccttcccg
     5461 gttttttctc atggttgggc cgcttattga aaagcaccag ccgctctaac gcaaatcacg
     5521 tgccagagcc gcgcgcaata gttcacatgc gctcggcgtg cgcgatcagg aagcggaaca
     5581 ccggcctcca ctccggcgcc atgcgggtga atacgtcacg gtgccgcatg aatgcggcga
     5641 gcgcagcctc gccctcgcgc gaaagaatgc ttgcggccat ggaagcggcg agcgcgtgcg
     5701 caacgagttc cggcgtaagg ttcgactccg cttcgagaat ggcctgcgcg gcgcgcgcca
     5761 tctcggccgg gcgagcggca gccaccgcgg cgtgcagccg cagccagcgc cgatcggcct
     5821 cggaaagatg cgccccgcac ggggtcgagg cgaggccagt ccagagagaa accgcggccg
     5881 ggggcggaag ctgcgcgccg acgaggcgcg cgagcgccgc gagctcgtgc ggcagcacgc
     5941 cttcgggaag tcgtacacgg cattcgacta gcgcggcgcg caacaggacg aggcttgcgg
     6001 ccggctgcgc ctcaacgcca gtgagaaacc gcgcgttacc ggaggcaagg aaatgctgcg
     6061 cggcatgcgc ttcccgcacg gatgcggcgc gcggcagcca gggtcgttcc cccgaactca
     6121 ggcgctgcgc ttcgggctgg cgccgcgcgc gctcgtcgaa cagcgggagc agcggcaacg
     6181 gcgcgtgcga aagcatggcg aaatccggcg ccgcgctttg catgtagcgc gcgagcggcg
     6241 ccttcatggc gagcgcggga aagtaatcgg aattgggcgg cacgcggaag ttcgcgaaat
     6301 acggcgcgag cgcggcgcgg ccggccacgc ggtgcagcgc cagatcttcc gggctcgcga
     6361 tactgaagcg ctcgagctcg gcgcgcagcg cggggtgcgc gagcgcgcgc gcatccggcg
     6421 ccggcaccga cccgttgtgc gcggcgacga tgacgaggtc cgcgctgccc ggaagccaga
     6481 attcgtagtc gctgaaattc gcgtgcagcg cagtcacgat ggtggcgagg agctcgggcg
     6541 tgatctcgta cagctgcacc cattgcaaaa gcaggccgcc gtctttgaga tggcgccgca
     6601 cgtcgcgata gaactcggcc gagaacaggc tcgcgacccc gctcacccag gggttggatg
     6661 gctcggacac gatcacgtcg tagcgctcgc gccgtacgga gaagaacgtc ttcgcgtcct
     6721 cgtagtggat gtggctgcgc gggtcctcgt aggcgcggcc gttggcccgg cggaacgccg
     6781 acaccgcctc gaccatggcc ggctcgatct cgatggagtc aacgcgctcg agctgcggcg
     6841 aggcgagcag gacgtgcgtc gtcatgccgg tgccgaatcc gatgttggcg acgcgccgcg
     6901 cctcgggtgc gagcaccagc ggcagcgcgc cgagtatggt catcgtcacc tcgtccggcg
     6961 ccggctcgcc gcggtccagg cgcagcgcgc cgtcgggctt gccgttcgtt cgcacggagt
     7021 acgttccgcg cgactcggtg atgctcacgg tggcggtctt gccgtgcagc tgccggtgca
     7081 gcgcatggtg ctcgggcagc aggccgccca tccggtatac ccccgatgcc atcaggcgcg
     7141 cgtcgagcgg gacgaggagc gcggcgccca gcgcggcggc cgcaacaccc gctgccacaa
     7201 gcggcacgcg gcggaagccg gccggcgccg ccgcgcgcag cagcaccacg ccgagcgcga
     7261 gatcgatggc cgcgccggcc gcgatcaggc cttccagccc gaggaccgtg aagccaacat
     7321 gcaccgcgag cagcacgccg acgatcgcgc cggcggtgtt cgccgcgtac acctgcccga
     7381 tggcgcgctc gcccgcacct tggcgcagca gggacaccgt gatgagcggc agcgtcatgc
     7441 cggcgcagaa ggccgccgga aacatgaccg ctaggcagag cgcgtggctc acgacgttga
     7501 aaaccagata gccggattcg gtgcgcgcga gcgcgtccag cgtcctcgcc atggcatgga
     7561 acgtccagcc gtagaccgga atggtggcga gggcggccac gcccatggcg agctgcaccc
     7621 acgcgaggaa gcgcaccgtg ctccctgcgg cgtcgatccg gcgccgcacc cagaggccgc
     7681 caaaggcaat gccgaggatg aatgcggaga gcatgagctc gaaggcgtgc gtcgagctcc
     7741 cgagcacgag cgagagcatc cggatccagc cgatctcgta catgaaggac gacatgccgg
     7801 tcagcgcggc gaccgcgagc aaaagcctaa ggtcggcgac ggaaatgcgc gccgttgtcg
     7861 gcgtcggttc cctgccgagc gcggcttttc gccccggcaa caacatgacc gccccggcga
     7921 cggcgaggtt gatgaccccc gctgcgacga gcgtgcccgg tagtccgacc gccgaaatga
     7981 agtagaagcc gctcgccagt acaccaaccg ccgcacccag gctgttggtg aagtaaagca
     8041 tcgctacggc atagccaggg cgcgccgggc cgaggcgcag cacgccgccc gtcatgagcg
     8101 ggaacgtcat gccgaggagc accgactgcg ggaggacgag cgcggcgccg agcgcccatt
     8161 taaaggcgtg cgcggccgcg ggcgagccga gcgccgggat cacgtggtcg aaagcggcgc
     8221 cggtcgcggc gacgaacagc gggtggaaga cggtcgctgc aatgccgacg accagctcga
     8281 tgagggcgta ggcaagcaga agatcgcgca ggcgttggga gagccggctc gcggcccagg
     8341 caccgagcgc catgccgccc atgaagatac cgagcacgag aggttgcgcg tacgcggagt
     8401 ggccgaggaa cagcttgaga tagtgcgtcc agaccgactc gtacatcagg ccggcgaagc
     8461 cggaggccgt gaacaccgcg agcgcgagcg gcaaaaacgc gcgcgcgccg ctcatggctg
     8521 gtagcccgga atgcgcgatt cgtcgacctc gtcgatctgc tccggctcga gaaacatttc
     8581 cgcgtagcgc cggtaaacct tcttgcgcac gaagatgtcg aagaggtcgg gatcgacgtg
     8641 gtggttgagc ttcatgcggc cgaggatctc gagcgactcg gagagcgtct tgcctttctt
     8701 gtagggacgg tcgcgcgcgg tgagcgcctc gaagatgtcg gcaatgccca tgcagcgggc
     8761 ctgcaccgac atctgctccc gcttgaggcc cctgggatag cctctcccgt ccatgcgctc
     8821 gtggtggccg cccgcgtatt ccggcacgtt cgcgaggtgc ttgggccacg gcagcgcctc
     8881 gagcatcttg atcgtggcga cgatgtggtg gttgatgatc ttgcgttcct cctccgtgag
     8941 cgtgccggcg cggatcgtga ggttcttcac ttcgtcgtcc gagaggaagt tcgcttcgtg
     9001 tcccgccacg tcgcgccagc ggtaggccgc gatccgcttt acccgcgcga catcctcatc
     9061 cttcatacgc tcgctgccca tgttgcaggc gtgcaggaag cgccggtcgt cgtccagctc
     9121 gcgcagccgc acgcgcaact tgcgctcatc gagctcgccg cgcagcgcct ggatctccag
     9181 atcgcgcttg agcacttcga accgtgtgtc gatgagcgtg atgcggtcgt agatggtctc
     9241 gagcttggtc gccttgtcca ccacgtgcac cggcgtcgtg accttgccgc agtcgtgcag
     9301 gaggcccgcg atcttgagct cgtagcggtc cttgtcggtc atctgaaact tggcgagcgg
     9361 accctcgtgc gtgtcgttca ctgcttcggc gagcatcatg gtgagctgcg gcacgcgctg
     9421 gcaatgcgcg ccggtgtacg gcgacttttc gtcgatcgcc gagttgatca gattgatgaa
     9481 ggactcgaac agctgctcga gctggttgat gagctgccgg ttgctgagca cgattgccgc
     9541 ctgcgaggcg agcgactcgg cgaggcgctg gtcggaggcg gaaaaggcga cgatctcgcc
     9601 gctgcgcggg tcggctgcgt tgatgagctg gagcacgccg atcgtctcgt gctcgtggtt
     9661 gcgcatcggc accgtgagga atgacttcga gcggtagccg gtcttcgcat cgaaggcgcg
     9721 cgtgccggag aaatcgaagc cctcggcacc gtaggcatcg gcgatattga ccgtcctgcc
     9781 ggtgagcgcc gcgtgcgcgg ctaccatgct gtggttcggc tccccatcgc ggtagagctg
     9841 gatcggatag aacggcaccg ggtttccact cgtgccgccg aggtagtaac gcagcgaggt
     9901 ggtgcgcacg atctcgaaac gcagcgtctt gtcgtcggcg acgcgataca gcgtgccgcc
     9961 gtcggcgccc gtgatcgact tggccgccgc gagaatgagc tcgagcagcc ggttgatgtc
    10021 gcgctccgcc gaaagcgagg caccgatcag gttcagcttc tcgagacgct ccccgaggtc
    10081 ggcgtcgcgg ttggccgccg cgcgcaggcg aaggccgtgt gtgttatcag cgggtttcaa
    10141 cgctcgccat tgctcttctt atttgatgca cactgcgcgc acttccttga tgtgggtgat
    10201 gccggccagg catttttcca tgccgtcctg cttgagcgtg cgcatgccct cctccagcgc
    10261 aatcgccagg acttcggcca cgcgcgcatg ctcctggatc gccttcttca ggcgatcgga
    10321 ggcgatcaga agctcgtgca ggccgcagcg ccctttgaag cctgagccgc cgcagcggtc
    10381 gcacccgacc ggcttgtaga aggtgagctg acccttgtcg ttgccgtaga ccttcaccca
    10441 ctcgcggtag acggcctcca ttgcgccctt ggggtcggcc ttgaagcggg gcgtgttcat
    10501 cagctcctcg cagtactcgc gcaggaacga ggtgagctcg gcggcctcgg gcgtgtaggt
    10561 ctgcttgcac ccgcacaggc gcttcgccag ccgctgcgcc aggatgccga gcaaagcatc
    10621 ggagaagttg aaagggtcca tccccatgtc gagcaggcgc gtgatggatt ccggtgccga
    10681 gttcgtgtgc agcgtcgcga acaccaggtg gccggtcagc gaggcttcga tgccgatgcc
    10741 ggtggtctcc ttgtcgcgca tctcgccgac catgatgatg tccgggtcgg cgcgcaggaa
    10801 agccttcatg acggtcgcga agtccagccc cgccttcttg ttcacctgca cctggcgcag
    10861 gcctttctgc gtgatttcca ccgggtcttc ggcggtccag atcttggtgt ccggggtatt
    10921 caggaacttc agcaccgaat gcagcgtcgt ggtctttccc gaaccagtgg gcccgcagca
    10981 gaaaaacagc ccgtaaggct tggacacggc ggtcttgaga tgctggaggt tgcgctcgct
    11041 gaagcccatc ttgtccaacg ggatcggctc gccggcagcg agaatccgca tcacgatgtc
    11101 ctcgatgccg ccggcggtcg gaatggtcgc aacgcgcagc tcgatatcga gcgggccgaa
    11161 tttcttgaac ttgatcttgc cgtcctgggg ctttctcttc tcggagatgt ccaggtcgca
    11221 catgatcttg atgcgtgcca cgatcgccgc gcggtagctt gccggtacct cgatgtacgg
    11281 cccgagcgag ccgtccttgc ggaagcggat ctccgtcttc gccttgccgg gatagggctc
    11341 gatatggatg tcggacgcgc cctggttata ggcgtcgacg atcaccttat tgacgagctt
    11401 gacgagctcg ttatcggccg ccgcggaggc ttcgtcgttc gacgcctcga ggatgtctcc
    11461 ggcatcgtcg gccatcgcac cgagcaggtc ctcgacgttg cccagatccg cggcgcccga
    11521 ctccgggccg tagaactggt tcaccgtgtc cttgaaatcc ttttgcgtgg tgaccttgta
    11581 gatgatgcgc gctttcggaa acacgttcgc cgcgatgcgc gaggagccaa tgcgctcggg
    11641 atcgaggcac agaaccgcga gcccctgcct gctgtcatcg atcggtaccc actggtttgc
    11701 ctcgacgtac tcccgcttca ggttcttcag cagatcgccg ggcttcacac gatccggctt
    11761 gaacggctcg tagggcacgc cgaagaactt ggacagcgcc gcgccgatgg ccgcgttatt
    11821 cacttggaac tcgtccacca ggatctgttc gatgtcgacc gcttttttcc gcgccttgcg
    11881 tgcggcgagc tcaaattcgc ccagcgagat caccgcatcg gcaattaggt agtcatactt
    11941 gctcttcgcg atctgcgact tctgtcgctg cttgaaggcg atggcgagcg tctgcgcgag
    12001 ctcgctcacc ccctcctcgg cgagcccgcc gaacggcacg ccggccttgt tgttgatgat
    12061 ctggatgacg ccgatcagtt cgttcgtccc ggcctcgagg atcggtgcga cgagcatctg
    12121 cttggtgcgg taaccggtgc gcttgtccac ttcctgcagg aagcgcaggt tggggttaag
    12181 accgcgcagc tcgttctcgt cgtagacgtc cctgacgttc gcggtccttt tcgccagcgc
    12241 cacgtaaccg gcgatcgagt gctcggcgat cggcagcttc aggtccttga acgaattcaa
    12301 gcccgtcttc accttgctga cgatggactg cttgtcctcg cccaccgagt agatggtgag
    12361 gcgatcggcg tggaacagtt gacagatgtc cgcgctcacc tcgagcatga tttcgtcgat
    12421 gttgctggtg gcgtggatct tgttcgtcac cgcctgcaac tgcttctgaa acgcgagacg
    12481 cgcgccgacg tcagtgtgcg cgggcgcgtg attcagaaca gcgctcatcc gcccctcccg
    12541 acccctactt gtgattagac ttgatatgtt acggctaggt ctctggattc tcaaggaaat
    12601 ccatgtttta cgagcgtgcg ttttgcccgc tttcgcggct ctttttcaag ccgtcctcca
    12661 gaagccatac cttgaagccg cgctgcgcga agagaaatgc cgccgccgcg ctgcgccgtc
    12721 cgctctggca ataggcgatg tactccttgc tccggtccag cacggcgaac atatttctaa
    12781 cttcggcgag gggcacgttg atcgccccgg caagcttgtc gtactggtac tccgagggat
    12841 atctcacatc gagccagagc gcgccgcggc gcaccttgtc gagcgcttct gcgtagccga
    12901 ggcgacgcag cagcggctcg cgcaatagtt cgttgaaatg ccggcggtcg agccgcagaa
    12961 gtcgcgcatc ggtgagcgcg gtgaccgtag cgttgcgctt cgcttccgag accagcgctt
    13021 cttcaccgaa cgcatcgccc gtcttcagct ccgcgagcac cacctcggcg ccaccgacgg
    13081 tgcgcctgac ctgaaagcgg cccgattcga tgacgtagta gtaatcgcct tcgtcgccct
    13141 cgcggatgac gacctcgccg cgcgcgcacg ggcggcgctc gaaacggcgg aaaagctcgt
    13201 cgatgcgcgc cggaggcaga ggcgcgaata tggtgcgcag gttcgatagc gaaaacgcac
    13261 cgctcgccgc catctcgcga ccgcggctcg ccgccacctg gtcccaggtc gcgaggatgt
    13321 cgagcaggtc gtcctcgagg accaagagat cgacatcggt gatggcgcgg gcgcgggcaa
    13381 cggtctcctt gcgccgattg agcgcgtgac gcgtttcttc gctgccaccg accactacga
    13441 gggtgccccc ggcctggaaa gaaagtagca gctcgccggc gagcaggaac accgactgct
    13501 tcgcgagcgt gcgtccgctg aacggatcgg cgccgcgcgg cgcgcgctcg atcgctgcga
    13561 cctcggagag ctcggcgaga cgctcgcgcg aaagcccggc caggggcgca agcgcagcga
    13621 gctggtccgg gctggcgttc agaattccag gacgaggttg ttttgcagca tgcgcggccg
    13681 gaacgcgccg gcgcattcct cgatctcgag catcgtcggc tcgatctcgc cgggcttgag
    13741 atgggtgatg tagatctcgg cgttgcgctc gagcttggcg agctcctcgg cgagcgtggc
    13801 ggggcacagg tggcgcgaga tctcggcgag ctggcgctcc ttgttcgaga acgcggtctc
    13861 gatgatgagg aacttcagat tggcgatgcg gttgacgatg cgccacagcg catcgttggg
    13921 ccccgtgtcc cccgtaaaca ccaggctggc tgcgccgctg tcgaggtgat agccgaccgc
    13981 cggcacggta tgacgcgcgg gaatcggcgt aatcgagcgc ccggcgagcg tgatggtggc
    14041 acccacctcg atctcgctgt agcgcatgaa gggcgcttcg gcgctcggga tttcgctgaa
    14101 atccggccag atcgcccagt tgaacaggtg gttcttcagg atctcgatgg tggcgcgcgt
    14161 ggcgtacagc gtgatcggct tggagcgcat gcccccgacc gtgtcgacga gaaacgggat
    14221 cgaggtgacg tggtcgaggt gcgaatgcgt gacgaagatg tggtcgatct gcgaaagctc
    14281 cgcgagcgtg agatcaccca cgccgcttcc cgcgtcgatc agaatgtcgc tgtcgagaag
    14341 gaaggacgtt gtgcgcaaat ggcgtccgcc gatccctccc gagcaaccga gcacacgaag
    14401 gcgcacttga ggcgtcttcc tatttctcgt cgaatttcgt gatgccgtcc caatcggacg
    14461 gcggcgggtc gcggcgcatg tcgtgtactt ttctcaagta caccccatag agcccgcacg
    14521 ccggatcggc tcgctgcagc cgttggagaa cggcctcggc ggcgtcccat ttgccgcgcg
    14581 cggtaagcgc gcagtgcctc cctccacgac tcgagctccg cgctctcttt ttccagaggc
    14641 tcatagatgg caatggcctc ctccttgccc ttgacccgga tgcggtcgat ctccctgaat
    14701 accacatctt ggatccggct gcgcgtagcc tctccgacaa ggatgccgac accgtacgtt
    14761 cttgtccggc cctccagccg tgaggcgacg tttacggcgt cgcccacggc cgtgtaggca
    14821 cggcgcacgc gcgagcccat gtcgcccacg cgcaccatgc cggaattcac gccgatgccg
    14881 atggccagac gcggcagccg cacctggcaa gccgcgaatt gagcgcatgg tccgcatcat
    14941 cgatgggcgc accccagaac gccatgatgg cgtcgccgat gtacttgtcg agcgtaccgc
    15001 cgtgccgctc acgaatcaca gcgctcattg cggtgaggta ctcgtcgatg tagtcgcgca
    15061 gggcttcggg gctcaggctt tcggaaatcc cggtgaagcc gcggatatcg gcgaacagat
    15121 cgtgagctcg gcgctcttgg gcttgtcgta gcgttcggga tcgagctcca tgcgaacact
    15181 tctggcgggg acgtattcgc ggaagcgcgc ggcgagccgg cgcttcaggc gtgactcgac
    15241 gaggtatccg tacgaacctg cggctccagg gccagcggcc gacttcggcg aggcttcttt
    15301 cgtcgatgtc caggatcgcg atgcgctcgt cgcgcccgcc cggcatggtc agcgtagccg
    15361 catgtcgtac aggatgttct ccagctggct gataagaaca ggcgtccgtc ggtcgacgga
    15421 tagcagtgca gagcgtgaag aacgacgaga agcgtgatcg cgaagccgag cgcgaccctc
    15481 aggacctaaa ccttgagaaa gaactccatc ttgacgccgg caagctcgat cacgtcgtgg
    15541 tccttgagcg cgtgcggcgc cgcgccgatc gcctgtccgt ttacgatggg ccggttcgcg
    15601 ccctcgacgt gggtaatgaa atagccctgt ggccgccgcg taagcaccgc cacctgcacg
    15661 ccgggcttgc cgagcgtggt gaggggcttg gtgagctcga gctcccggcc ggcattggcc
    15721 ccggaaagaa gctggatcgc gccgagcggc tgcgccgggc gcgcaggggc agcgggtgca
    15781 gcggcgggag gcggcggcgc aggcttgacc gcgtcctttt cggcgggctg cggtgccgac
    15841 gcgctcaccg gcgcggccgc gcgtagagcg accgccgcgg cagcgacgcc gccgccggga
    15901 ttcgcggcgg gcgccttaac ttcggctgcg gctctcaccg ccgccggacg cagcaccatc
    15961 gtcttctcga agtcggcctt gtcggcggcg gcggcgggcg ccgcctcgcc gacgaacttg
    16021 agcttgtact tgccgagctc gatgacgtcg ttgttctgca ggaagtgctt cttgatcggg
    16081 ttgccgttca ccacggtccc gttggtgctg cccaggtcct cgaggaaaga atcgttgagg
    16141 atggtgacga ttacggcgtg ctcgccgctc accgcgaggt tgtcgatctg gatgtcgtta
    16201 tggggctttc ggccgatgct ggtgcgctcc ctggtcaagg gaatctcctt gagcaccagg
    16261 ccatccatgc tgagtatcag tttagccata gccgatcccg gggggttatt ttagccagcc
    16321 gaacaccttg gccatgacgc cgcggggtcc ggggtattcc cgtagaacgc gcacgagtat
    16381 aaccgaaacg ttatcccggc ccccgtggtc gttggccatc tgcaccagct gctgcgcggc
    16441 gagcttgaga tttccaccga gcgtctgcac ggtcatgccg atgtcctcgt catcgaccat
    16501 gtcgcagagg ccgtcggagc agagcaggta gatgtccccc gtcttcgtcg aatactcgcg
    16561 aatctcgggc tcgacggtcg gatcgatgcc gagcgccttg gtcacgaggt tcttgtgctg
    16621 cgccttcttt gcctgctccg gagtgatgat gcccgagtca atctgctcct gcagcaaaga
    16681 atggtcgcgg gtcacctggc tgaagcggcc ttcgcgcaac atgtagacgc gggaatcgcc
    16741 gaggtgcgcg accagcacgc ggttgtcgta gaagaggcac acgacgagcg tggtacccat
    16801 gccggcatac tggggctggc tttgcgcggc atggtagatg gagctattcg ctttcaggac
    16861 ctgctcgcgc accagtcgcg cggcgagctg ctggtttgtg cgcgagtcga tgtcgtaggg
    16921 cttggcgctt tgcaggatct ggcgcatctc ggagaggatc accgtggtcg ccatgccgct
    16981 cgccacctcg ccggcgttgt acccgcccat gccgtcggcg agcaccacca gcccgttcgc
    17041 gggatcggcg gcaatcgaat cttcgttatg cgaacgcacc atgcccgggt cggtgcacga
    17101 tgcgatttcc aggctctggc tgagatccat ccgtccccgg gtcgtcgtcc ccggggcgca
    17161 ttgttgcagc cctatgctaa gagcgtcaac gatttagctg aggatctgtc ccgcgcgcag
    17221 cgctccggcg gcgagcaatt cggtcgccgc gactttcttc ccgtcggcga gctttgcgcg
    17281 catcacttcg atgcgaccgc cctgcgcggt gacctgaaaa gaattgtcgg tgacctcgac
    17341 gatttccccg atcttgccct tgaccgcgcc aaacgtgcgc accgggtgct tgcgcgcgtc
    17401 gtagatctga agctccttgc cctcgagcgt gcaccacgcc cccggcgccg gattgcaccc
    17461 gcggatcacg ttgtagacga aatcgacgtg gttcgcccag ttgatgcgcg cctcctgccg
    17521 gcggcaccag ccttcgtaag tcgcctgcga ctcgtcctgc accgcttcct tgtgcttgcc
    17581 ggcgacgacg aggtccgcgg cctcgagcat cgccttcacg cccatcggga acagccgatc
    17641 gaagtacacc gtgcccagcg tatcgtcggg cccgatcgcg gtttccttct gcaggatcac
    17701 cggcccttcg tccagaccat cggtggggcg gaagatcgtc agtccggtcc tcgcgtcgcc
    17761 gcggatgatc ggccagttga tcgaagacgg accccggtac ttgggcagga gcgacgggtg
    17821 gtactggatc gtgccgcgct tcggtatgcg cacgaaatcc tgcggggcaa actgcaggac
    17881 gaacgccatg acgccgagct cgacgttcag gtccttcagc gctttcttcg cctcctcgct
    17941 cttcagcgta ggaaactgga ataccttcac acccttttcc tgcgccgcca ccttgagcgc
    18001 gtcaggccgc gcgcccggct tctccggcgc gcaaaacacc ccggcgactt cgtccttgcg
    18061 ggcgagaaac gcctcgagca ccgccttgcc gaagtcctgc tgcccgataa tcgcgatttt
    18121 catgccgctt tcttcggcgg caccgaaaag gcgccggcgc ctctcagatt cttgatcttc
    18181 ccctcgtcgt agccgagcac ttcccgcaga atttcgtcgg tgtgctcacc aagcgtaggc
    18241 gagcgcttga ttctcgccgg ggagtccgag agcttgatcg gcatgcccac gttgaaccat
    18301 ttgccgcgct gcggatggtc gagctcgacc cacatgtcgc ggccgcgcac gtgctcgtcg
    18361 ttcgcgaggt cttcggtcga catgatcggg ccgcacggca cgtcgagcgg gttcaggatc
    18421 gccatgagct cgcgcttggt gtacttctcg gcaaacttgt tgatgagcgt ccacatcagg
    18481 ctctggttcc tgcgccgctc gccgatcttc gcgaagcgcg cgtcgctcgc catgtcggac
    18541 atgccgactt ccggcccgat gcgcttggcg aggtcttccc aaaccgcttc ctgcaccacg
    18601 acgtagagcc agtcgtttgc gccgtgcggc ttgcagtgga tcgcattgcc gagctgcccg
    18661 ccgccggaat cattaccagc gcgcggcacc tcgcccatgc ccttgtaggt cggtaccgag
    18721 tactcggcca gctcctgacg ctgcaggcgc tggtggtccc ggaacttcac gcggcagagg
    18781 ttcatgaccc cgtccatcat ggcgacttcc acgtactggc ccttgccggt tttctccgcc
    18841 tggcgcagtg cggcgagcag gccgatcgcg aggtgcaggc cggtgccgct atcgccgatc
    18901 tgagcgccgg tgacaaacgg cgggccgtcc ggcaccccgg tggtgctcat ggcgccgccc
    18961 atcgcctgcg ccacgttctc gtaggccttg aactcggcgt acgggccggt ggaaccgaag
    19021 cccttgatcg agcccatgac gatcttcggg ttgatctcct tgatcttctc ccacgggaaa
    19081 ccgaagcggt cgagcacgcc cgggccgaag ttctccatga tgatgtcgca cttctttacg
    19141 agatccacga agacctgctt gccctcgctc gtcttcatgt tgacggtgat cgagcgcttg
    19201 ttgcagttga gcatcgtgaa gtagagcgaa tcggcgttcg gcacgtcgcg cagctgcccg
    19261 cgcgtcgcat cgccctgcgg gttctcgaac ttgatgacgt cggcgcccag ccaggccaaa
    19321 agctgactgc aggtcgggcc ggcttgcacg tgggtcatgt ccaggatgcg gactcccttc
    19381 agagcttcca ttactttttc ttccccgcgg ttggattgag actggtgatg cgcccgcttt
    19441 ccgtgccggc cgtctcatcg atgatggcat tgatcagcgt cggcttcttc gagcgcagcg
    19501 cctcttccat cgccttgcgc aactcgcccg gtgtcttggc gtagacgccg acgccgccga
    19561 atgcctccat cagcttctcg tagcgcgcat ccttcacgaa cacggtgggc gcgacatcgc
    19621 cgccgcgcgg gttgacgtcg gtgcccttgt acacaccgtt gttgttcatg accaccacgc
    19681 acaccggcag gttgtagcgg cagatcgtct ccacctccat gccggagaag ccgaaggcgc
    19741 tgtcgccctc gaccgcgatc accggctgct tggtggtgac cgcggcggcg acgcagtagc
    19801 ccatgccgac gcccatgatg ccccaggtgc cgacgtcgat gcgcttcctc ggtttgtaca
    19861 tgtcgacgat cgagcgtgtg aagtcgagcg cgttggcgcc ttcgtttacc aggatcgctt
    19921 ccgggttcgc tttcacgatg tcgcgcacca cgttgagcgc gctgtggaag ttcatgggcg
    19981 ttgggttctt ggcaagcgtc tcggccatct tggcgacgtt ggcattcttc ttctcggcga
    20041 cggcgttcag ccattccgcc ggcggcttcg cccagttgcc gagaccggcc agcagcgcgc
    20101 tcacacatga accgatgtcg cccaccacgg gcgcgtcgat ggccacgttc gagtccgctt
    20161 cctgcggcga gatatcgatc tggatgaatt tgtggccacc cctgtccttg ggcgccttgc
    20221 caccccaggt cttgcccttg ccgtgcgaca ggagccagtt cagccgggcg ccgatcagca
    20281 tcaccacatc ggcgccaggc agcacgaacg agcgcgcggc cgacgcgcac tgctcgtgcg
    20341 tgtccggcag caggcccttc gccatcgaca tcggcaggta cgggatgccg gttctctcga
    20401 ccagtgcctt gatttcggca tcggcctgcg catacgcggc gcccttgccg aggatgatga
    20461 gcggcttctt cgcgctcttc agaagatcaa gcgcgcgctt caccgcctcc ggcgcgggaa
    20521 tctgcttcgg cgccgggtcc accaccttga tgagggtctt cttcgccgtg gcggcgtcga
    20581 ttgcctgcgc gaagagcttg gcgggcagat ccagatagac cccgcccggg cggccagata
    20641 gcgccgcgcg gatcgcgcgc gccacgccga cgccgatatc gtcggcgtgc agcacgcgat
    20701 aggcggcctt cgcaaccggc ctggcaatcg caagttgatc catctcctcg taatcgccct
    20761 gctgcaggtc cacgatctcg cgctcggagg accccgagat caggatcatc gggaagcagt
    20821 tcacggtagc gtgcgcgagc gcgttcagtc cgttgaggaa gcccggcgcc gacaccgtca
    20881 gacagatgcc cggcttctgc gtgaggaatc ctgcgatcga agccgcgtac ccggcattct
    20941 gctcgtggcg gaacgagagc acgcgcatgc cgtcggcctg cgcgcggcgc gtgaggtcgg
    21001 tgatcgggat gccaggcagg gcgtagatcg tgtcgatgcc gttcagcttg agcgcatcga
    21061 tgacgagctg aaagccgtcg gtcgattgcg atgcctccac ggcgacttgg ggttctgcgg
    21121 tgcccatgag gtgcctccta agggggaacg atatggtaaa aacgcgtgca gtcccgcagc
    21181 cttgactgaa gtcaagccga atctaattga ccgcgccaat tgacctcgct cgcctcgcat
    21241 ccgcaacgca acagcctgcg cctgcagcta cggaataacg cgcttctgca gggattgagc
    21301 ccggcgcagt gggcggagct cgagccgctg ctcgtggtgg ccgattaccg caagggcgac
    21361 ccgcttgtgc gccagggcga cgaagagatg cagcagctct tcatcctcga gggcatgctg
    21421 aagcgcgtgg tgtccaatcc gcagggccgg gagatgattc tgcgcttcgc cgccgagggc
    21481 gagatggata cgtcgtacgc cgcctggcgc ctgcgcacgc cggtgcccta ctcgatcgtc
    21541 gccgtcacca aggtgcgtac cgtcgagctc gccatgccgc tctgggtgga gttccttgaa
    21601 aaaaataccg acgtgaaatc acgcttcgag tacgaggtaa tgaagctcat gtcggaagtg
    21661 atggcacaca cgatcaccct gcacctgctc gacgcgcccg ggcgcctcgc ccgcttccag
    21721 agaaagcacc ccgagctcgc gggacgcatc ccgaaaaagg agctcgccgc ttacctgaac
    21781 ctcacccccg agacactctc gcgcctgaag cattccaacc gcggccgggt gcgccccggc
    21841 ttatgaacga cgcagcttgg cgatggccaa ggagaggagc ggccagaaaa ggagcagcag
    21901 cgcaagggtg gtcatgaagc cgaccagctt cttcgagaac aggatcgaga gatcgccctg
    21961 cgacatgagc atcgcctggc ggaacgacga ctcggcgttg tcgccgagca cgatcgcaag
    22021 caccagcggc gcgagcggat actgcagctt cttgaagagg tagccgagga tcccgaatac
    22081 caccatcatc cacacgtcga agatcgcatt cttgacggtg aacgagccga tcgcgcagat
    22141 caccatgatg atcggcgcaa tcacggagaa cggcacgcgc aggatcgccg cgaagagcgg
    22201 cacgcaggtc agcaccacga tcagcccggc gatgttgccc aggtacatcg aagcgatcag
    22261 gccccagacg aaatcctttt gctccacgaa cagcagcggc ccgggctgca ggccccagat
    22321 catcagcccc ccgagcagca ctgccgccgt gggcgagccg ggcacgccca gcgtgatcat
    22381 cggcagcagc gccgccgtgc cggcggcgtg cgccgcggtc tcgggcgcga ccacgccttc
    22441 cactttgccg gtgccgaact tgtcgccgtc cttcgagaag cggcgcgcca cgccgtagct
    22501 catgaatgag gccggcgtcg cgccgccggg cacgatgccc atgaagcagc cgaccgcgga
    22561 ggagcgcagc gaagtcatcc agtaacgcgg cagctccttc caggtgtcga gcaccacgcg
    22621 cacgttgatc ttcgccgatg ccccccggaa ggcgagcccc tcctcgagcg tgagcaggat
    22681 ctcgccgatg ccgaacaacc cgatcaccgc gaccaggaaa tcgaagccgg tgagaagatg
    22741 cgtcgagccg aaagtgagcc gcagcgtgcc ggtaacggtg tcggtgccca ccgcggcgag
    22801 cgcgaagccg agcatcatgg cggcgatggt cttgaagggc ggctccttgc tcgtgccgac
    22861 gaaggcgcag aaggtcagga gatacaccgc gaagaactcg ggcggaccga attccagcgc
    22921 gaatcccgcc accaccggcg acaggaacgt gatgacg
//
LOCUS       DASTPD010000274         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_757367, whole genome shotgun sequence.
ACCESSION   DASTPD010000274 DASTPD010000000
VERSION     DASTPD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757367"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFR57_09105"
     CDS             <1..718
                     /locus_tag="VFR57_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259560.1"
                     /translation="DGYTLLGNSSAHVANPSIYKTLPYDTLKDFANIAPLAGGPNVLI
                     VSPQTGWKSFADFVAAAKAKPGQLNFSSAGVGSGTHFNLEKLKLMAGIDVAHVPYKGT
                     PEAIGDTIAGRVCCYWAPINAALPHVRGGKALALGVSSAQRSSLLPDVPTLAEQGVPG
                     FDYTLWVGLWGQAAMPAELVNKINADVNKALASPDLADRLTKLGTIPMKMSSAEFAQF
                     VRKEVEDTAKVLKAAGIQPQ"
     gene            715..1668
                     /locus_tag="VFR57_09110"
     CDS             715..1668
                     /locus_tag="VFR57_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259561.1"
                     /translation="MNRILVAFIAACALSANAQTYPTKPVQVIISFTPASATDIVGRI
                     VTAKLSEYWGQPVVNENRSGAGGSIGSAAVAKAAPDGYTLLINSNAHSVNPAIYAKLP
                     YETTKDFTDIAPLSELPNVLVVSAESRYKTLAELVGFGKANPGRINFGHAGVGSGTHL
                     NTVKLVTAAGIDVTQVPFKGTPEVVAAILSGNVDCYWVPISAGIANIKAGKLRALAVS
                     TAKRSALLPDLATTAEGGVKGADFGLWFGMWGPAGMSAELTAKINADVRKALADSGVR
                     EKLANTGNSPLDMSPQEFARFVRSEIDDYQRVVRAAGIKPQ"
     gene            complement(1655..2305)
                     /locus_tag="VFR57_09115"
     CDS             complement(1655..2305)
                     /locus_tag="VFR57_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEU0259562.1"
                     /translation="MKKLDRKQMAARVARDIPEGSYVNLGIGLPTLVADFLPKGREII
                     LHSENGILGVGPKPTPGEEDEDLINAGKEPVTLLTGGSYFHHGDSFGMIRGRHLDFAI
                     LGAFQVSVAGDLANWHTGAPDAIPAVGGAMDLAVGAKKVLVMMEHLTKSGESKIVERC
                     TYPLTGARCVDRIYTDLAVIDVKAEGLRVVETVEGLSFEELQRLTGVPLKRIATAA"
     gene            complement(2305..2964)
                     /locus_tag="VFR57_09120"
     CDS             complement(2305..2964)
                     /locus_tag="VFR57_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEU0259563.1"
                     /translation="MINKFVASAEAALADVPDGATIMIHGFGNAGMPAALIDALIAQG
                     AKDLTIVNNNAGNADTGLAALIGARRVRKILCSFPRQADSWHFDRLYRAGEIELELVP
                     QGTLAERIRAAGAGIGAFFTPTAYGTRLAEGKETRRIGERDYVLEFPIHADYALIKAD
                     RADRWGNLTYRKSARNFGPIMAAAAKCTVVQVRETVELGAIDPEHVVTPGIFVKRIVK
                     H"
     gene            complement(3032..>3268)
                     /locus_tag="VFR57_09125"
     CDS             complement(3032..>3268)
                     /locus_tag="VFR57_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259564.1"
                     /translation="SAMARGHSDATLRVERDDRGTVECAPHMASSATFSYLLPLYGQR
                     ASRSSGISPATTMTYSDFLLPKIQPTTVFIFRQL"
BASE COUNT          581 a         1075 c         1048 g          564 t
ORIGIN      
        1 cgacggctac acgctcctcg gcaattcgtc cgcgcacgtc gccaacccat ccatctacaa
       61 gacgcttccc tacgacacgc tcaaggactt cgccaacatc gcgccgctcg ccggcggccc
      121 gaacgtcctt atcgtctcgc cgcagaccgg gtggaagtcg ttcgcggatt tcgtcgccgc
      181 cgcgaaggcg aagcctggcc agctcaactt ctcctccgcc ggtgtgggca gcggcacgca
      241 cttcaacctc gagaagctca agctcatggc gggcatcgat gtggcgcacg tgccgtacaa
      301 aggcacgccg gaagcgatcg gcgacaccat cgccggccgg gtgtgctgct actgggcgcc
      361 gatcaacgcc gcgctgcccc acgtgcgcgg cggcaaggcg ctcgcgctcg gcgtgtcgag
      421 cgcgcagcgt tcgagcctct tgcccgacgt gccgacgctc gccgagcagg gcgtgcccgg
      481 atttgactac acgctgtggg tcgggctatg ggggcaagcg gcgatgccgg cggagctcgt
      541 caacaagatc aacgccgacg tgaacaaggc gctggcgagc cccgacctcg ccgatcggct
      601 gaccaagctc ggcaccatcc cgatgaagat gtcgagcgcc gaattcgcgc agttcgtgcg
      661 caaggaagtc gaggacaccg ccaaggtcct caaggccgcc gggatccagc cgcaatgaac
      721 aggatccttg tcgccttcat tgcggcgtgc gcgttgtcgg cgaacgctca gacctatccg
      781 acgaagcccg tccaggtgat catctctttc acgccggcga gcgcgaccga catcgtcggc
      841 cgcatcgtta ccgcgaagct ctcggaatac tggggacagc cggtggtcaa cgagaaccgc
      901 tccggggccg gcggctcgat cggctctgcg gcggtggcga aagcggcgcc cgacggctac
      961 acgctgctca tcaactccaa tgcgcactcg gtgaacccgg cgatctacgc caagctgccg
     1021 tacgagacca cgaaggactt caccgacatt gcgccgctct ccgagctgcc gaacgtgctg
     1081 gtggtcagcg ctgagtcccg ctacaagacc ctcgccgagc tcgtcggttt cggaaaagcg
     1141 aacccgggca ggatcaactt cggccacgcc ggggtcggca gcggcacgca cctcaacacc
     1201 gttaagctgg tcacggctgc cggcatcgac gtgacccagg tcccgttcaa gggcacgccg
     1261 gaagtggtcg cggcgatcct gagcggcaac gtcgactgct actgggtgcc gatctccgcc
     1321 ggcatcgcga acatcaaggc cggcaagctg cgtgcgctgg cagtgagcac cgccaagcgc
     1381 agcgccctgc ttcccgatct ggcgacgacc gccgaggggg gagtgaaagg cgccgatttc
     1441 gggctatggt tcggcatgtg ggggcccgcc gggatgagcg cggagctcac cgcgaagatc
     1501 aacgccgatg tgcgcaaagc gctcgccgat tccggggtgc gcgagaagct cgccaacacc
     1561 ggcaactcgc cgctcgatat gtcgccgcag gagttcgcgc gcttcgtgcg cagcgaaatc
     1621 gacgactacc agcgcgtggt gcgcgctgcc ggaatcaagc cgcagtagcg attcttttca
     1681 gcggtacccc ggtgagccgc tggagttctt caaaactcaa accctcgacg gtctccacga
     1741 cacggaggcc ttcggccttt acgtcgatta cggcgagatc ggtgtagatg cggtcgacgc
     1801 agcgcgcgcc ggtgagcggg tacgtgcagc gctcgacgat cttgctttcg ccactcttcg
     1861 taaggtgctc catcatcacg agcacctttt tcgcgccgac cgcgaggtcc atcgcaccgc
     1921 cgaccgcggg gatcgcatcc ggcgctccgg tatgccagtt ggcgaggtcg cccgcgaccg
     1981 agacctgaaa cgcaccgagg atggcgaagt cgaggtggcg gccgcggatc atgccgaacg
     2041 aatcgccgtg gtggaagtat gagcctccgg tgaggagcgt caccggctcc ttgcccgcat
     2101 tgatcaggtc ctcgtcctcc tcgccgggcg tgggcttggg cccgacgccg agaataccgt
     2161 tttcgctgtg caggatgatc tcgcgccctt tgggcagaaa gtcggcgacc agcgtcggca
     2221 ggccgatgcc gaggttgacg taggagcctt ccgggatgtc gcgcgccacg cgcgccgcca
     2281 tctgcttgcg atcgagcttc ttcatcagtg cttgacgatt ctcttgacga aaattcccgg
     2341 cgtcaccacg tgctccggat cgattgctcc gagctcgacc gtctcgcgca cctgtaccac
     2401 ggtgcatttc gcggccgccg ccatgatcgg accgaagttg cgcgcgctct tgcggtaggt
     2461 gagattgccc cagcggtcgg cgcggtccgc cttgataagc gcgtagtcgg cgtggatcgg
     2521 aaactcgagc acgtaatcgc gctcgccgat ccggcgcgtc tccttgccct ccgcaagcct
     2581 ggtgccgtac gcggtcggcg tgaagaaagc gccgattccc gcgcccgccg cacggatgcg
     2641 ctcggcgagc gttccctgcg gcacgagctc gagctcgatc tcgcctgcgc ggtagagccg
     2701 gtcgaagtgc cacgagtccg cctgccgcgg gaacgagcag agaatctttc gcacgcggcg
     2761 cgcgccgatc agcgcggcaa gccccgtgtc cgcgttcccg gcgttgttat tgacgatggt
     2821 gagatccttc gcgccctgag cgatcagcgc atcgatcagc gccgccggca tgcccgcgtt
     2881 gccgaagccg tggatcatga tcgtggcgcc gtccgggacg tcggccaggg cagcttcggc
     2941 agaggcgacg aacttattga tcatgcgagc cgtgaattat ggcactggcg tttcttgcgc
     3001 ctgccttttg acaatagacc ttcaatcagc ctcataactg tctgaaaata aagacagttg
     3061 tcggctgaat tttcggcaat aagaaatcgc tgtaagtcat tgtcgtggct ggagaaatcc
     3121 cgcttgaccg cgacgctctt tgtccgtaga gtggcaaaag gtaggaaaaa gtggcggaag
     3181 aagccatatg gggagcgcat tccacggtgc cgcggtcatc acgctcgacg cgaagggtcg
     3241 cgtcgctgtg cccacgcgcc atcgcgga
//
LOCUS       DASTPD010000275         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_347536, whole genome shotgun sequence.
ACCESSION   DASTPD010000275 DASTPD010000000
VERSION     DASTPD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347536"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VFR57_09130"
     CDS             <1..1828
                     /locus_tag="VFR57_09130"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0259565.1"
                     /translation="AVDAAFARAAQRVELELVNNRLCGAAIETRGAIATGDTLYCGTQ
                     TPHHIRSHVCAELGLTENELRVACYDMGGGFGYKGKHYPEETIVVWAARRLRRPVKWI
                     ATRNESFVSDTQGRDHRTRASLALDRNGRFLALRVQTRADLGAYVSTFGAAIPGPIYS
                     ALLAGLYRTPAIHIEVTGVFSNSVPTDAYRGAGRPEACYVLERLADKAARALGIDRAE
                     IRRRNLIPRDAMPYRTPIGPTYDCGDFPRIFERALAASDYENFEQRRKEAKTRNRLRG
                     IGLACYLESSGVAPSRLAGMMGARGGFHEAATVRVAPDGSVTAYLGTHNHGQGHSTTF
                     AQILADRLGVPLERISVVEGDTALVPVGTGTFGSRSIAVGGSALHSACQKVILKGRKI
                     AAHLLEASAADIEFSAGEFRVAGTDRKIVLRDVALAAYVPHHYPLESLEPGLDETAYY
                     DPPNFAYSNGVHVCELEVDPETGRVELVSYHAVDDIGTVINPMIVEGQLHGGLGQGIG
                     QALAERTVYEAGQLLSASFMDYALPRASDLPRFFSETDESQPCTHNPLGAKGCGESGA
                     IGAPAAVMSALLDALQIEHLEMPATPHTVWGAMMRARNAGAP"
     gene            1836..>2554
                     /locus_tag="VFR57_09135"
     CDS             1836..>2554
                     /locus_tag="VFR57_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259566.1"
                     /translation="MIQELNPAKLALLYRREFEMCQVKKEETIVAVSDLATRREYVQA
                     AFAAADDLGADIYELCVNSLPSWTKVGVPTIGKCRGTLEAVKAADMVVIFHVPLFTKW
                     LGQVMDGGTRVLMIIDSPDDLEQLMSPPGLKDACKYAERIYSSTKRVRVRSEAGTELG
                     YECGEYPVMTQWGFADERGHFDHWGGGHIHTFPNEGSAHGTVVFQPGDIVILPYCRYI
                     ADAVRLEIREGHIVKLEGGMDA"
BASE COUNT          465 a          827 c          838 g          424 t
ORIGIN      
        1 ggcagtcgat gccgcgttcg cacgcgccgc gcagcgcgtc gagctcgagc tcgtcaacaa
       61 ccggctctgc ggagcggcga tcgagacgcg cggcgcgatc gccacaggcg acaccttgta
      121 ctgcggcacc cagacgccgc accacatccg gagccacgtg tgcgcggagc tcggtctgac
      181 ggaaaacgag ctgcgggtcg cgtgttacga catgggaggc ggcttcggct acaagggcaa
      241 gcactatccc gaggaaacta tcgtcgtctg ggctgcgcgc cgtctccgcc ggccggtgaa
      301 gtggatcgca acgcgcaacg aatcgttcgt ctccgacacc cagggcaggg accaccgcac
      361 gcgggcctcg ctcgcgctcg accgcaacgg acgctttctc gcgctccggg tgcagacgcg
      421 cgccgatctc ggcgcgtacg tgtcgacctt cggtgcggcg atccccggac cgatctacag
      481 cgcgctcctc gctggccttt atcgcacgcc tgcaatccat atcgaggtta cgggcgtgtt
      541 ctcgaacagc gtacctaccg acgcctatcg cggcgcgggc cggcccgagg cgtgctacgt
      601 gctcgagcgc ctcgccgaca aggcggcgcg cgcgctcggc atcgatcgcg ccgaaatccg
      661 gcgccgcaac ctgatcccca gggacgccat gccgtacagg acgccgatcg gaccgactta
      721 cgactgcggc gatttcccgc gcatcttcga gcgcgcgctg gccgcttctg actacgagaa
      781 tttcgaacag cgaagaaagg aagcgaaaac ccgaaaccgg ttgcgaggca tcgggctcgc
      841 ctgttacctg gagtcctcgg gcgtcgcgcc ctcgcgcctc gccggaatga tgggcgcgcg
      901 cggaggcttc cacgaagcgg caacggtgcg cgtggcgcct gacgggagcg ttaccgccta
      961 tctaggcacg cacaaccatg gtcagggcca ctccaccacc tttgcccaaa tcctggccga
     1021 tcgtcttgga gttcccttgg aaagaatttc agtggtcgag ggagacacgg cgctcgttcc
     1081 ggtgggcacc ggtacctttg gatcccgctc gatcgcagtg ggcggctcgg cgcttcattc
     1141 cgcctgccag aaggtgatcc tcaagggcag gaagatcgcc gcgcacctgt tggaagcgtc
     1201 cgccgccgac atcgagttct ccgccggaga attccgggtt gcaggaacgg accgcaagat
     1261 tgtccttcgg gacgtcgcgc tcgcagcgta cgtcccgcac cattatccgc tcgagtcgct
     1321 ggagccggga ctggacgaga cggcttacta cgaccccccg aacttcgctt attccaacgg
     1381 cgtgcatgtc tgcgagctcg aagtcgatcc ggaaaccggc cgggtcgagc ttgtctctta
     1441 tcacgcggtg gacgacatcg ggacggtgat caacccgatg atcgtggaag gtcagctgca
     1501 cggcggtctc ggccagggca tcgggcaggc gctcgcagag cgcacggtgt acgaggccgg
     1561 gcagcttctt tccgcctcct tcatggacta cgcgcttccg cgcgcgagcg atctgcctcg
     1621 gttcttttcc gagaccgacg aaagccagcc gtgcacgcat aacccgctcg gcgcaaaggg
     1681 gtgcggggaa tcgggcgcga tcggggcgcc ggcggcggtg atgagcgcat tgcttgatgc
     1741 actgcaaata gagcatctcg aaatgccggc tacgccgcac accgtatggg gggcgatgat
     1801 gcgcgcccga aacgcaggcg cgccataatg cgcgaatgat ccaggagctt aacccggcga
     1861 agctcgcgct gctttaccgc cgcgagttcg agatgtgcca ggtaaagaag gaagagacga
     1921 tcgtcgcggt cagcgacctc gccacccggc gcgagtacgt gcaggcggcg ttcgctgcgg
     1981 ccgatgacct cggtgcggac atctacgagc tgtgcgtgaa ctcgctgccc tcgtggacca
     2041 aggtgggcgt gccgacgatc ggtaaatgca gaggcacgct cgaggcggtc aaggccgcgg
     2101 acatggtggt gatcttccac gtgccgctct ttacgaaatg gctcgggcag gtcatggacg
     2161 gcggcacgcg cgtgctcatg atcatcgact cgcccgacga cctcgaacaa ttgatgtccc
     2221 cgcccgggct caaggacgcc tgcaaatacg cggagcgcat ctacagcagc accaagcgcg
     2281 ttcgcgtgcg gagcgaagcg ggtaccgaac taggttacga atgcggcgag taccctgtga
     2341 tgacgcaatg gggcttcgcg gacgagcgcg gtcatttcga ccattggggc ggcgggcaca
     2401 tccacacttt tccgaacgaa ggcagcgcgc acggcacggt ggtgttccag ccgggcgata
     2461 tcgtcatcct gccctattgc cgctacatcg ccgacgcggt gaggctcgag attcgcgaag
     2521 gtcacatcgt caagctcgaa ggcggcatgg acgc
//
LOCUS       DASTPD010000276         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_347555, whole genome shotgun sequence.
ACCESSION   DASTPD010000276 DASTPD010000000
VERSION     DASTPD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347555"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..631)
                     /locus_tag="VFR57_09140"
     CDS             complement(128..631)
                     /locus_tag="VFR57_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259567.1"
                     /translation="MPLTELNHYLLVAKNLERTKKFYQDVLGMELADRPDFGFPGYWL
                     KLGDDICVHLASQKPNTVRDTFLLKKHPRGTTGSGSVDHIAFLAKDPNDVRERIQKHK
                     IPMHFRSFPDAKLFQIFLKDPDDVTIELNFLGEVIDENEWKGKGSASAVTLDRSAGKR
                     TAVRKQN"
     gene            complement(671..1594)
                     /locus_tag="VFR57_09145"
     CDS             complement(671..1594)
                     /locus_tag="VFR57_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259568.1"
                     /translation="MLVAGAAWAQDYPNRTVRVVVPFPPGGAPDLVGRTLANRLSERL
                     GQAFVVENRTGAGGNIAAEAVAKSAPDGYTLLATSDGPLVINPSVYPTMPFDTLRDFV
                     PISIAASAGLVLMACPSLAVSSVSDMIALAKKKKLTYASSGYGSSQHVAAELLKDSAT
                     IDLTHVPYKGFGAAVGDAISCNVDLIFGAISTGIPHVRAGKLKPIAVTQPRRHAGLPD
                     TPTFSETGHPAVVIEAYMGLLAPSGTPRLIVDKLHTEVAAILLEKETAERLTGAGLDL
                     VRSTPEQYATRIRADLVKYAKLSKALAAKSE"
     gene            complement(1608..2783)
                     /locus_tag="VFR57_09150"
     CDS             complement(1608..2783)
                     /locus_tag="VFR57_09150"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0259569.1"
                     /translation="MTDLSTVGYEEAMRRAREIVPVLLERAQKCEDARVLLPENEKLL
                     HETGLFRFHQPKRFGGMELPFVAVVDIVSELARGCPSTAWNVGNLGCHHWILGYYEPE
                     TQHELWDQNPDVLIASSIALAAGRGRKAKDGFVVSGRWPFSSGVDNSDWNMLAVTIYD
                     EEKAVDWRLCLVPKSDYKVIDTWYAMGMIGTGSKDIEVKEVFVPERRALALARCRGGL
                     EHPGARLNSGPLFRVPIVASAGHPLSATALGTAEGAFEHVLQTFKTRVGTYTGAKVSD
                     FQAVQVKLARARGLIDSARCLMRESALVFQHNENLALEEKLRLRAQNALAVAQAREAV
                     ETLWSFYGANAIYIRDPLQRYLRDVQAASQHFSFNFDIAGSAYGTVALGGKYANPTM"
     gene            2782..4134
                     /locus_tag="VFR57_09155"
     CDS             2782..4134
                     /locus_tag="VFR57_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0259570.1"
                     /translation="MRDSRVTRYPMGMKDMNCLPLSTRSAGAAECYNSALSAYLSNRA
                     DFSANLKALLEADPEFALGHCFRGYGAMLMFNRAMLPLAADAHRAARNLAPGATRREQ
                     AHVRALGRWAAGDVDGALQEWEALLAEWPHDALAIRLAHFNYFWTGRAADMRASLERV
                     AARWSDALPAYATLLSCLAFALEETGDYGAAERAGRRAVEIDPAEVWGTHAVAHVLEM
                     QGRREEGIAWLAGLERHWEGKNNFVHHLWWHRAMFHLELRETDAVLELYDKRFRNLAS
                     PLVAAMPDLYIDVQNAASMLFRLERQGVDVGARWSELADKAQARIGDCQSAFTLPHWM
                     MALAATGRDAAARSMLDSMKSHPTASVREVAFPVCEAVLAHRRGDYARAVVCLASVLD
                     RLHQLGGSHAQRDVLHQLYFDACLKSQRSAEARALLDSMAARFPVPPSRRVGYAAAVT
                     "
     gene            complement(4127..>4654)
                     /locus_tag="VFR57_09160"
     CDS             complement(4127..>4654)
                     /locus_tag="VFR57_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HEU0259571.1"
                     /translation="QNDVYAVLWSAAGGFGDPLERDQARVEADIANGDVSEKAALEIY
                     GVVLGDAKATEGQRNKLRRRRIHGHSRKAEKLSAPVKFLSTENVAVHTNGHYACAKCG
                     TDLGPVSGNYKSSCVRNDLPIEASNPIVGDPKRFIDPLPQFRQFCCPGCGLAIENEIA
                     VAEDPVLRDVEILSR"
BASE COUNT          764 a         1467 c         1568 g          855 t
ORIGIN      
        1 cttgccaagg tggacgagat gccagtcggt gacgtggccg tcctcggccg agtactggca
       61 catcggcgag acgacgatgc gatttcttat ccggatgtca cggatgcgga tctcgtcgaa
      121 cagcatgcta gttttgcttg cgtaccgcgg tgcgcttgcc ggccgagcgg tcaagcgtca
      181 cggccgaggc cgaacccttg cctttccact cgttttcgtc gatcacctcg ccgagaaagt
      241 tgagctcgat ggtcacgtcg tcggggtcct tgaggaagat ctgaaagagc tttgcgtccg
      301 ggaagctacg gaagtgcatc gggatcttgt gcttctggat ccgctcgcgc acatcgtttg
      361 gatccttggc gaggaaggcg atgtggtcta ccgagcccga gccggtcgtc ccacgagggt
      421 gtttctttaa caggaaagtg tccctaactg tgttcggctt ctgcgaggcg aggtgcacgc
      481 agatgtcgtc gccgagcttg agccagtatc cggggaatcc gaagtcgggg cggtcggcta
      541 gctccatgcc gagcacgtcc tggtagaact tctttgtgcg ctcgaggttc ttggcgacta
      601 gcaggtaatg gttgagttcg gtaagtggca cggcgcttct ccaaagtagg aaccgatccc
      661 gtattttgtc ttattccgac tttgcagcga gcgcctttga aagcttcgca tatttcacga
      721 ggtcggcgcg aatacgcgtc gcgtactgct ccggagtgct gcgcacaagg tcaagccccg
      781 cgccggtcaa gcgctcggcg gtttccttct ccagcaggat ggcggcgacc tctgtgtgga
      841 gcttgtcgac gatgagccgc ggcgtgcctg acggggcgag aagccccatg taggcctcga
      901 tcacgacggc ggggtggcca gtctcgctaa aagtcggcgt atcaggcagg ccggcatggc
      961 gcctgggctg tgtgaccgcg atcggcttga gcttcccggc gcggacatga gggatcccgg
     1021 ttgagatggc gccgaagatc aggtcgacgt tgcagctgat cgcgtcgccg actgcggcgc
     1081 cgaagccttt gtagggaaca tgcgtcaggt caatcgtcgc cgaatccttg aggagctcgg
     1141 ccgcgacgtg ctggctgctg ccgtagccag aagatgcgta ggtcagcttt ttcttctttg
     1201 cgagcgcaat catgtcgctc acgctcgaga cggctagtga cggacaagcc atcaacacaa
     1261 gtcctgcgct cgccgcgatg gagatgggta cgaagtcgcg cagcgtgtcg aacggcatgg
     1321 tcgggtacac gcttgggttg atgacgagcg gcccgtcgga agtggcgaga agcgtgtagc
     1381 catcgggcgc cgacttcgcg accgcctcgg cggcgatgtt gccgccggcg ccggtgcgat
     1441 tctcgaccac gaacgcctgg ccaaggcgct cgctcagccg gttggcaagc gtgcggccaa
     1501 cgagatccgg cgcgccgccg ggcgggaagg gcacaacgac acggacggtc cggttggggt
     1561 aatcctgcgc ccaggcggcg ccggcgacga gcaacagcag aagaatgcta catagtcgga
     1621 ttcgcatatt tgcccccgag cgcgacggtg ccgtaagctg agcccgcgat atcaaagttg
     1681 aaagagaagt gctggctcgc cgcctgcacg tcgcgaaggt agcgctggag cgggtcgcgg
     1741 atatagatcg cgttcgcacc gtagaacgac cacagcgtct cgaccgcttc gcgcgcctgc
     1801 gcgacggcca gggcattttg tgcgcgaagg cgcagctttt cctccagggc caggttttcg
     1861 ttgtgctgga acaccagcgc cgactcgcgc atgaggcagc gtgcagaatc gatcagacct
     1921 cgcgcccgcg cgagcttcac ctgcacggcc tgaaagtcgg agactttggc gccggtatat
     1981 gttcctaccc tcgttttgaa cgtttgcaac acgtgctcga aagctccctc ggcagtcccc
     2041 aaggcagtgg ccgagagcgg atgccccgcc gaggcgacga tcggcacgcg gaatagcggg
     2101 cccgaattga gtctagcgcc cggatgctcc agccctccgc ggcagcgggc gagggcgagc
     2161 gcgcggcgct cgggtacgaa gacctccttt acctcgatat ccttcgagcc ggtgccgatc
     2221 attcccatcg cgtaccaggt atcgatcacc ttgtagtcgc tcttcggcac gaggcagagc
     2281 cgccagtcca ccgctttctc ttcgtcgtag atcgtgaccg ccagcatgtt ccagtcggag
     2341 ttgtccaccc cggaagaaaa aggccatctt ccggagacga cgaacccgtc cttcgctttt
     2401 ctgccgcgcc cggccgcgag cgcgatggac gaggcgatga ggacgtcggg gttctggtcc
     2461 caaagttcgt gctgggtctc gggctcgtag tagcccagga tccagtggtg gcatccgagg
     2521 ttgccgacgt tccaggccgt cgacgggcag ccgcgcgcca gctccgacac gatgtcgacg
     2581 accgcgacga atggcagctc catgccgccg aagcgcttgg gctggtggaa gcggaaaagg
     2641 ccggtctcgt ggagcagctt ctcattctcg ggcaggagta cccgcgcgtc ctcgcatttc
     2701 tgagcgcgct cgagcagcac cggcacgatc tcgcgcgccc ggcgcatcgc ctcttcgtac
     2761 ccgaccgtgg acaagtcggt catgcgcgat tctagagtta cgcgctaccc tatggggatg
     2821 aaagacatga actgcctgcc gctcagcacg cggtctgccg gggccgccga gtgctacaac
     2881 agcgcgctca gcgcgtacct aagcaatcgc gccgacttct ccgcaaacct gaaagcgctg
     2941 ctcgaggcgg acccggagtt cgcgctcggc cactgcttca ggggctatgg cgcgatgctg
     3001 atgttcaacc gggccatgct gccgctggcg gcagacgcgc accgcgccgc gcgaaacctg
     3061 gcgccgggcg ccacacggcg cgagcaggcg cacgtccgcg cgctcggccg ctgggctgca
     3121 ggcgacgtgg acggcgcgct gcaggagtgg gaggcgctgc tcgcggagtg gccgcacgac
     3181 gcgctggcga ttcggctcgc gcacttcaac tacttctgga cggggcgcgc ggcggacatg
     3241 cgcgcttcgc ttgaacgcgt ggcggcccgc tggagcgatg cattgccggc gtatgcgacg
     3301 ctgctctcgt gcctcgcgtt cgcgctggag gagactggcg actacggcgc cgccgagcgc
     3361 gccgggcggc gcgcggtcga gattgatccg gctgaggtat ggggcacgca cgcggtcgcg
     3421 cacgtgctcg agatgcaggg ccgccgcgag gagggaatcg cctggctcgc cggcctggag
     3481 cggcattggg agggcaagaa caacttcgtg caccatctct ggtggcatcg cgcgatgttc
     3541 caccttgagt tgcgtgagac cgacgcggtg ctggagctct acgacaagcg ctttcgcaac
     3601 ctggcctcac cgctggtcgc ggcgatgccc gatctttaca tcgacgtcca gaacgcggcc
     3661 tcgatgctct tccgcctcga gcggcagggc gtggatgtcg gcgctcgctg gagtgagctc
     3721 gccgacaagg cgcaggcacg cattggcgac tgccagtcgg ccttcacgct gccgcactgg
     3781 atgatggcgc tcgctgccac ggggcgcgac gccgccgcgc gttcgatgct ggattccatg
     3841 aaaagtcatc caacggcctc ggtgcgcgag gtcgcgttcc cggtttgcga ggcggtgctc
     3901 gcgcaccgtc ggggcgacta cgcgcgcgcg gtggtctgct tggcgtccgt gctcgatcgg
     3961 ctgcaccagc tcggcggcag ccacgcgcag cgcgacgtgc tgcaccagct gtacttcgac
     4021 gcgtgcctga agtcgcagcg gagcgccgag gcgcgcgcgc tgctggactc gatggcggcg
     4081 cgcttccccg tgccgccgtc ccgtcgcgtc ggctacgccg ccgccgttac ctagagagaa
     4141 tctccacgtc gcgcagcacc gggtcctccg ccacggcgat ctcgttctcg atcgcgagcc
     4201 cgcagcccgg gcagcagaac tgccggaact ggggcagggg atcgatgaag cgcttcggat
     4261 cgcccacgat cgggttcgag gcctcgatcg gcaggtcgtt gcgcacgcag gagctcttgt
     4321 agtttccgct aaccggaccc aggtcggtgc cgcacttggc gcacgcgtaa tggccattcg
     4381 tatggaccgc gacgttctcg gtggagagga acttgactgg cgccgacagt ttttccgctt
     4441 tccggctgtg tccatggatg cgccgcctgc ggagcttgtt gcgctggcct tcggtcgcct
     4501 tggcatcgcc gagcactacg ccatagatct ccaacgcggc cttttcggac acatcgccgt
     4561 tcgcgatgtc ggcctcgacc cgtgcctggt cgcgctcgag cggatcaccg aagccgcccg
     4621 cggcagacca cagcacggca taaacgtcgt tttg
//
LOCUS       DASTPD010000277         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1165869, whole genome shotgun sequence.
ACCESSION   DASTPD010000277 DASTPD010000000
VERSION     DASTPD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165869"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFR57_09165"
     CDS             complement(<1..338)
                     /locus_tag="VFR57_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEU0259572.1"
                     /translation="MEDVPTLEATNRILARAQRSTMDVPPMDNSAMDGFAVRTGDGER
                     LRVTQKIMAGSTGKPLEPGTAARIFTGAPIPPGADAVVMQEDTAADAEFVLIKKNPKP
                     GDWVRYVGSD"
     gene            complement(407..898)
                     /gene="mobB"
                     /locus_tag="VFR57_09170"
     CDS             complement(407..898)
                     /gene="mobB"
                     /locus_tag="VFR57_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU0259573.1"
                     /translation="MKIFGFAGWSGSGKTTLIEQLIPRFVQRGLRVSLVKHAHHTFDV
                     DQPGKDSYRHRQAGASEILVTSSRRWVLMHELRGAHEPPFEEQVKRMSPCDLLLVEGF
                     KFAPIPKLEVWRAETGEPLLHPNDPHIVAVASDAKVETKLPLLDLNDVDGIASFILKK
                     LEL"
     gene            complement(895..1641)
                     /locus_tag="VFR57_09175"
     CDS             complement(895..1641)
                     /locus_tag="VFR57_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0259574.1"
                     /translation="MEEFVVAALAAGMLVAAFGYASVGHGGASAYLAAMALAGIAPPE
                     MRPVALLLNILVSSLATWKFWRAGHFRWRLFWPFAAVSTPLAYVGGAITLPGQAYKVL
                     VGLVLLYAAWQLWHSVRAGDEMRELREPPLGWAMLVGAAMGLLAGLTGVGGGIFLSPL
                     LLMLGWAGTKQTSAVAAPFILVNSIAGLAAIVLAKSAPLPSYVWILAPAVIIGGWLGA
                     EYGSRRFANPVVRRVLAVVLALAGAKMIWV"
     gene            complement(1655..2014)
                     /locus_tag="VFR57_09180"
     CDS             complement(1655..2014)
                     /locus_tag="VFR57_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0259575.1"
                     /translation="MARRKKGRYGGLTLRVLGKRAPAMGPGKAELIERIAETGSISGA
                     ARAMGMSYRRAWQLVEALNRDYRERVVDTATGGKRGGGARVTPFGRQVLSLFRGMEEK
                     ASAAIASDLRRFHRYIP"
     gene            complement(2015..>2300)
                     /locus_tag="VFR57_09185"
     CDS             complement(2015..>2300)
                     /locus_tag="VFR57_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0259576.1"
                     /translation="PALNTASGMNAYILADRGTWLSFKNRGELAIVLEGDKRLFNQYG
                     IILVNPARHLGVKRDMGQAFIDWIISTEGQEAIAAYKIDGEQLFFPNAAQ"
BASE COUNT          404 a          775 c          734 g          387 t
ORIGIN      
        1 atatcgctgc ccacgtagcg tacccagtcg cccggcttcg gattcttctt gatcaagacg
       61 aactcagcat ccgcggcggt gtcctcctgc atcactaccg cgtcggcacc cggcggaatc
      121 ggcgcgccgg taaagatgcg cgcggcggtt cccggttcca ggggcttgcc tgtcgaaccc
      181 gccatgatct tttgtgttac acgcaagcgc tctccgtccc cggtcctgac tgcaaatcca
      241 tccatggcgc tgttgtccat cggcggcacg tccatggtcg agcgctgcgc gcgcgcgagg
      301 atccggttgg tcgcctcgag tgtgggcacg tcttccattt ctgcgaccgg cttcgcttcc
      361 gcaagcaggc gcgcgagcgc gtcgtcgacc gagagcagtc cctggttcac agctcgagct
      421 tcttcaagat gaagcttgcg atgccgtcca cgtcgttgag atcgagcaat ggcagcttgg
      481 tttccacctt cgcatcgctc gccacagcga cgatgtgcgg gtcgttgggg tgcaggagcg
      541 gctctccggt ctcggcgcgc cacacttcga gcttgggaat cggcgcgaac ttgaagccct
      601 cgaccagcag caaatcgcac ggcgacatgc gcttcacctg ctcttcgaat ggcggctcgt
      661 gcgcaccgcg cagctcgtgc atcagcaccc agcgccgcga cgaggtgacg aggatttccg
      721 aggcgccggc ttggcggtgg cggtaggagt ccttgccggg ctgatcgacg tcgaacgtgt
      781 ggtgcgcgtg cttgacgagc gaaacgcgca gtccgcgctg cacgaagcgc ggaataagct
      841 gctcgatgag cgtggtcttg ccgcttcccg accagccggc gaagccgaag atcttcatac
      901 ccagatcatt ttcgcaccgg cgagggcaag cactacggcg agcacacgcc gtacgaccgg
      961 gtttgcgaaa cgccggctgc cgtattcggc gccgagccag ccgccgatga tcaccgccgg
     1021 agcaagaatc cacacgtagg acggtagcgg agcgcttttc gccaagacga tggctgcgag
     1081 tcccgcgatc gaattcacca ggatgaaagg tgcggcgacc gctgaggtct gcttggtgcc
     1141 cgcccagccg agcatgagga gcagcgggct gagaaatatg ccgccgccga cccccgtgag
     1201 gccggcgagt aggcccatcg ccgcgccgac gagcatcgcc cagccgagcg gcggctcgcg
     1261 cagctcgcgc atctcgtcgc ccgcgcgcac gctgtgccag agctgccagg cagcgtagag
     1321 cagcaccagc ccaaccagaa ccttataggc ctgtccggga agcgtgatcg cgccgccgac
     1381 atatgcgagc ggggtcgaga ccgcggcgaa cggccagaac aagcgccagc ggaaatggcc
     1441 cgcacgccag aacttccacg tcgcaagcga ggagaccagg atattgagca gcagggcgac
     1501 cggtcgcatc tccggcggcg cgattccggc gagcgccatc gcggcaaggt aggccgaggc
     1561 gccgccgtgt cccaccgagg catagccgaa cgcggccacc agcatcccgg cggcgagcgc
     1621 ggcaacgacg aattcctcca cgcaccgcta tatatcatgg aatataacga tggaaacgcc
     1681 gcagatcgct ggcaatggcc gcggaggcct tttcctccat gccccgaaag agggacaaga
     1741 cctgcctgcc gaaaggcgtt acgcgcgccc cgccgccgcg cttgcctccc gtcgcggtat
     1801 ccacgacgcg ctcgcggtag tcccggttga gcgcctcgac cagctgccag gcccgccggt
     1861 acgacatgcc catcgcgcgc gccgcgcccg agatcgatcc ggtctcggca atgcgctcaa
     1921 tgagctccgc cttgcccggc cccatcgcgg gtgcgcgctt gccgagaacg cgcagagtga
     1981 ggccgccgta tcgccccttc tttcgtcgcg ccatctactg cgctgcgttc gggaagaaga
     2041 gctgctcgcc gtcgatcttg taggcggcga tcgcctcttg cccttcggtg gagatgatcc
     2101 agtcgatgaa tgcctgtccc atgtcgcgct tgaccccgag atgcctggcc ggattcacga
     2161 ggatgatgcc gtactggttg aacaggcgct tgtcgccttc gagcacgatc gcgagctcgc
     2221 cgcgattctt gaacgacagc cacgtgcccc ggtcggcgag gatataggcg ttcatgcccg
     2281 atgcggtatt cagcgccggt
//
LOCUS       DASTPD010000278         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_348638, whole genome shotgun sequence.
ACCESSION   DASTPD010000278 DASTPD010000000
VERSION     DASTPD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348638"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /gene="cca"
                     /locus_tag="VFR57_09190"
     CDS             complement(<1..395)
                     /gene="cca"
                     /locus_tag="VFR57_09190"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878672.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEU0259577.1"
                     /translation="MKVYLVGGAVRDELLGLPVKERDYVVVGSTPEEMVRLGFKPVGK
                     DFPVFLHPKTRDEYALARTERKSGRGYKGFTVYASPEITLEEDLKRRDVTINAMAKAE
                     DGTLIDPFGGKKDLESGVLRHVSEAFAEDP"
     gene            complement(402..1070)
                     /locus_tag="VFR57_09195"
     CDS             complement(402..1070)
                     /locus_tag="VFR57_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0259578.1"
                     /translation="MALVIGNKNYSSWSMRPWVLMREAKIPFEEVPLKFDESGGGLRV
                     AGIERYSAAGKVPVLLIDGEPVWDTLAICETVAELYPAKQLWPRDERARRVARSVCAE
                     MHSGFQALRGAMPMNIRGRYPGKGLNEKSGKDIQRVVAIWSDCRARFGKGGPFLFGAF
                     GIPDAFYAPVVMRFQTYAVEVPPAARAYCEAVQSLAALREWCDAARREIEFVAEDEPY
                     AKRN"
     gene            complement(1087..2475)
                     /locus_tag="VFR57_09200"
     CDS             complement(1087..2475)
                     /locus_tag="VFR57_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0259579.1"
                     /translation="MLLPRSLFGRTLLALAAGLALAQLGSAAIHLFDRGSSVYRLASL
                     QIAARIGQAARILDRLPPAERHKVVEEISGRHLRVSLPEGPVAVENGFSEHDAYERQF
                     SESLQKQIGARWPVRVDINPKPRTRGFVGEGLAAGPFELWMARHFYFLMPEPFSIVVQ
                     VGLQDGTVAVFDVNVPQEPLRRFESLAPILLLLLLVCFGLAAFLVRMTTRSLDRLARA
                     ADAIGQDPASAPLREAGPSEVRRVIAAFNRMQERVRRYLGERGRLLGAISHDLKTPIT
                     RLRLRAEMLPDADVRTRMLRDLDEMQTMVGETLDFFSSVGKGTQRQPVDVGALIESVC
                     EDRHEAGQALTVRGAPLGPYRADPQGLRRCLDNLIENAIRYGGSAEIEIADSAQHLRI
                     AVRDRGPGIPDPELERVFEPFYRLDDSRNMESGGTGLGLGIARNIARWHGGDVTLRNA
                     APGLVAELALPR"
     gene            complement(2475..3191)
                     /locus_tag="VFR57_09205"
     CDS             complement(2475..3191)
                     /locus_tag="VFR57_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0259580.1"
                     /translation="MERPDHVLVVDDDAEIRKLLGEYLQRNGFRVSLANDGREMRRSL
                     EQTRPDIVVLDLMLPGDSGLTLCRDLRAGSSLPVIMLTARAEEVDRIVGLEMGADDYL
                     AKPFSPRELLARIKTILRRTRGLPPASGDTRRVRFAGWTLDAAARQLVAPDGVVVALS
                     GAEFRLLTVFVEHPNRVLDRNQLMDLTVGRDGTPLDRSIDVQVSRLRVRLRDDAREPR
                     IIKTVRNEGYVLAATVERLT"
     gene            3329..3499
                     /locus_tag="VFR57_09210"
     CDS             3329..3499
                     /locus_tag="VFR57_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259581.1"
                     /translation="MKATLAAVLAALFGLIALSAHAAPLAQSGVQPLDIQAPLPADDD
                     KDKDKDKDKKDG"
     gene            complement(3570..4088)
                     /gene="gpt"
                     /locus_tag="VFR57_09215"
     CDS             complement(3570..4088)
                     /gene="gpt"
                     /locus_tag="VFR57_09215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU0259582.1"
                     /translation="MARVPYEDEIIISWVELHRDARYLSEVLHQKGPWKGIIAVTRGG
                     LVPAALVARELDIRLVDTICVTSYDSGPAGAAAQQQGKVQVLKGVSGEGEGYLLIDDL
                     VDTGKTAQVVRQLLPRAHFACLYAKPAGRPVVDTFVKAFKQTKWIHFPWDIEYKFATP
                     IKDGGRVAPGPG"
     gene            complement(4097..4870)
                     /locus_tag="VFR57_09220"
     CDS             complement(4097..4870)
                     /locus_tag="VFR57_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259583.1"
                     /translation="MDSREFALKMLSPLAFVVLLFVSWESICKALDVPLTILPAPSDV
                     FGALWQYRKAIWDNSFVTLWTTLAGFLIATVFGLGLGIAIGWHRALYAGIYPVLIGFN
                     SIPKVAVVPVLILWFGLGEIPAVLTAFLISFFPIVVNVATGLATTEPELEDVLRALGA
                     SKLDIMRKVGIPRSLPYFFGSLKVAITLAFVGAVVSETVGANKGIGKLMLDAQAGFQV
                     PIVFAGLLVLAVLGILLYAATAAIEARFTGWAFRGQANR"
     gene            complement(4863..>5350)
                     /locus_tag="VFR57_09225"
     CDS             complement(4863..>5350)
                     /locus_tag="VFR57_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259584.1"
                     /translation="NVLLPLEIVEPYRSNFGRRKFSGKAKELLHSVGLAGFTEKHPWE
                     LSGGMQQRTSICRALIHEPEILLLDEPFAALDAFTREELWSALRDLQAARKVTVMLVT
                     HDLREAVFLADTVYVMSSRPGRIVRRCEVPFPRPRDLELTYTPEFQDIVHELRSLIGR
                     G"
BASE COUNT          959 a         1795 c         1735 g          861 t
ORIGIN      
        1 gggtcctccg caaatgcttc gctcacgtgg cgcaatacgc ccgactcgag atccttcttg
       61 ccgccgaagg gatcgatcag agtcccatcc tcggccttcg ccatcgcgtt gatggtgaca
      121 tcccggcgtt tgaggtcttc ctccagcgtg atctcgggcg aggcgtagac cgtgaaccct
      181 ttgtagccgc ggccgctttt gcgctcggtg cgcgcaagcg cgtactcatc gcgtgttttg
      241 ggatgcagga atacgggaaa gtccttgccg accggcttaa agccgagccg caccatctct
      301 tcgggagtcg aacccaccac gacgtaatcg cgttccttaa ccggaaggcc gagcagctcg
      361 tcgcgtaccg caccgccgac gaggtagacc ttcacccgaa tctaattcct cttggcgtac
      421 ggctcgtcct cggcgacaaa ctcgatctcg cgccgtgcgg catcgcacca ctcccgcagg
      481 gcggcgagtg actggactgc ttcgcagtat gcgcgcgcgg ccggaggcac ttcgaccgcg
      541 taggtctgga agcgcataac caccggcgcg tagaaggcgt ccgggatgcc gaacgcgccg
      601 aaaagaaacg gcccgccctt tccgaagcgc gcgcgacaat ccgaccagat ggcaacgacg
      661 cgctggatgt ctttgcccga cttctcgttc aggcccttgc ccggatagcg gccgcggatg
      721 ttcatcggca tcgctccgcg cagcgcctgg aagcccgaat gcatttctgc gcagaccgag
      781 cgcgccacgc gccgcgcgcg ttcgtcccgc ggccaaagct gcttcgcggg gtaaagctcg
      841 gccactgtct cacaaatcgc cagcgtgtcc cacaccggct cgccgtcgat cagcagcacc
      901 ggcaccttgc ccgcggccga gtaacgctcg atgccggcca cgcgcagccc gccgccgctc
      961 tcgtcgaact tgagcggcac ttcctcgaac gggatcttgg cttcgcgcat gagcacccag
     1021 ggacgcatcg accaggacga atagttcttg ttgccgatga ccagggccag cttcacgcgc
     1081 caaagtctag cgcggcagcg cgagttcggc gacgagtccg ggggcggcgt tgcgcagcgt
     1141 cacgtcgccg ccgtgccagc gggcgatgtt gcgcgcgatg cccaggccga ggccggtgcc
     1201 cccgctttcc atattgcgcg aatcatcgag ccggtagaaa ggctcgaata cccgctcaag
     1261 ctctggatcc ggtatgcccg ggccgcggtc acggacagcg atcctgaggt gctgggcaga
     1321 atcggcgatc tcgatttccg ccgagccccc atatcggatc gcgttctcga tcaggttgtc
     1381 gaggcagcgg cgcaggccct gcggatcggc gcggtaggga cctagcggag caccacgcac
     1441 cgtgagagcc tggccggctt cgtggcgatc ctcgcagacg ctttcgatca gggcgccgac
     1501 gtccacgggc tggcgctgcg tacccttgcc tacgctagag aagaaatcca gcgtctcccc
     1561 caccatggtc tgcatctcgt cgaggtcgcg cagcatcctc gtacgcacgt ccgcgtcggg
     1621 gagcatctcg gcgcgcaggc gcaggcgggt aatcggcgtt ttcaggtcgt gcgagatggc
     1681 gccgagcagc cggccgcgct ccccaaggta gcggcgaacc cgttcctgca tgcggttgaa
     1741 ggcggcgatg acgcggcgaa cctcgcttgg cccggcttcg cgaagcggtg cgctcgccgg
     1801 gtcctggccg atggcgtcag ccgcgcgcgc gaggcggtcc agcgagcgcg tggtcatgcg
     1861 aacgagaaag gccgccaagc cgaaacacac aagcaacagc agcaggagga tcggcgcgag
     1921 cgactcaaag cgcctcaacg gctcctgcgg cacgttgaca tcgaacaccg ctacggtgcc
     1981 gtcctgcagc ccgacctgca ccacgatgga gaacggctcg ggcatcagga aatagaagtg
     2041 gcgtgccatc cacaactcga acgggccggc ggccaggcct tcgccgacga agccgcgcgt
     2101 ccttggcttc gggttgatgt cgacgcgcac cggccatcga gcgccgatct gcttttgcag
     2161 cgactcgctg aactgccttt cgtacgcgtc gtgctcggaa aagccgttct ccaccgcgac
     2221 cggaccctcg ggaagcgaga cgcgcaggtg gcgcccgctg atttcctcca cgactttgtg
     2281 acgctcggcg ggcggcaacc ggtccaggat gcgcgcggct tggccgatac gcgcggcgat
     2341 ctgcagcgag gcgaggcggt agaccgagct gccgcggtcg aagaggtgga tggcggcgct
     2401 gccgagctgc gcgagcgcga ggcccgctgc gagcgcgagc agcgtgcgcc cgaatagcga
     2461 gcgcggcaga agcatcaggt caggcgctcc acggtcgcgg cgagcacgta gccctcgttg
     2521 cgcaccgtct tgatgatacg cggctcgcgt gcgtcgtcgc gcaaccttac gcgcagccgg
     2581 ctcacctgca cgtcgatcga gcggtcgagc ggcgtgccgt cgcgcccgac ggtgagatcc
     2641 atcagctgat tgcggtcgag cacgcggttt ggatgctcga cgaacaccgt gagcagcctg
     2701 aactcagccc cgctcagcgc cacaacgact ccgtcgggcg cgaccagctg ccgcgcggcg
     2761 gcgtcgagcg tccaaccggc gaagcgtacg cgccgcgtgt cgccacttgc gggcggaagg
     2821 ccgcgcgtgc gccgcaggat ggtcttgatg cgcgcgagga gctcgcgtgg gctgaacggc
     2881 ttcgcgaggt aatcgtcggc ccccatctcg aggccgacga tgcggtcgac ctcctccgcg
     2941 cgcgcggtca gcatgatcac cggcaggctc gagcccgcgc gcaaatcgcg gcatagcgta
     3001 aggccgctgt ctccgggcag catgagatcg agcaccacga tgtccggccg cgtctgctcg
     3061 aggctgcggc gcatttccct gccgtcgttc gcgagcgaca ctcgaaagcc gttcctctgc
     3121 aggtactcgc ccagcagctt gcggatttcg gcgtcgtcgt cgaccaccag gacgtggtcc
     3181 ggccgttcca tcgcggcgct atatctgcac cgaggcgacc ggtgcgtcaa gtgatgtaac
     3241 acagtgtgtc aagcgccgcg gcgtgcaatc ctgcggttac aagcggtcgc tttaatcctc
     3301 gggtcgtacc caacccacag gagatccaat gaaagccacg cttgccgccg tactcgccgc
     3361 gctgttcggc ctgatcgcac tgtccgccca tgctgcgccg ctggcacagt cgggcgtgca
     3421 gccgctcgac atccaggcgc cgctgcccgc ggacgacgac aaggacaaag ataaggacaa
     3481 ggacaagaag gacgggtgag ctgagaccac cagatcagcg ctgaattcct ccctggtgtg
     3541 actcgagccc cgcttgcggg gctctttttt tagccgggcc caggggcgac gcgtccgccg
     3601 tccttgatcg gcgtggcgaa cttgtactcg atgtcccacg ggaagtggat ccacttcgtc
     3661 tgcttgaacg ccttgacgaa ggtgtccacc accggccggc cggccggctt ggcgtagagg
     3721 caggcaaaat gcgccctggg aagaagctgg cgcaccacct gcgcagtctt tccggtatcg
     3781 accagatcgt cgatcaggag atagccttcg ccctccccgc tcacgccctt aaggacctgg
     3841 accttcccct gttgctgcgc ggccgcacct gccgggcccg agtcgtagct cgtgacgcaa
     3901 atcgtgtcga ccagccggat gtcgagctcg cgcgccacga gcgccgcggg cacgaggccg
     3961 ccgcgcgtaa cggcgatgat gcctttccag gggcctttct ggtggagcac ttcggacagg
     4021 tagcgcgcgt cgcggtgcag ctcgacccac gagatgatga tctcgtcttc gtagggtaca
     4081 cgcgccatgc aagattctag cggtttgcct gcccgcggaa agcccagccg gtgaagcgcg
     4141 cttcgatcgc cgcggtcgcc gcatagagaa ggatgccgag caccgccaac accagcaggc
     4201 ccgcgaacac gatcgggacc tgaaacccgg cctgggcatc cagcatcagc ttgccgatgc
     4261 ccttgttcgc gcccacggtt tcggacacca ccgcgccgac gaaggcgagc gtaatcgcca
     4321 ccttgagcga gccgaagaag taaggcagcg agcgcgggat gcccaccttg cgcatgatgt
     4381 cgagcttcga ggctccgagc gcacgcagga cgtcctcgag ctcgggctcg gtagtcgcga
     4441 gcccggtcgc gacgttgacc acgatcggaa agaaggaaat gaggaacgcc gtaagcaccg
     4501 ccgggatctc gccgaggccg aaccaaagga tcagcaccgg caccaccgcg accttcggga
     4561 tcgagttgaa gccgatcagc accgggtaga tcccggcata gagcgctcgg tgccagccga
     4621 tcgcgattcc gagcccgagg ccgaacaccg tggcgatcag gaacccggcg agcgtggtcc
     4681 agagggtgac gaacgagttg tcccagatcg ccttcctgta ctgccacagc gcgccgaaca
     4741 cgtccgaggg cgcgggcagg atggtgagcg gcacatcgag cgctttacag atgctctccc
     4801 aggaaacaaa gagcaaaacc acgaaggcta gcggggatag catcttgagc gcgaactcgc
     4861 ggctatccac ggccgatcag gcttctcaat tcatgcacga tgtcctgaaa ctccggggtg
     4921 taggtcagct cgaggtcgcg cggccgcggg aacggcacct cgcagcggcg cacgatgcga
     4981 cccgggcggc tggacatcac atacaccgtg tccgcgagga acaccgcttc gcgcaggtca
     5041 tgcgtcacca gcatgacagt gaccttacga gccgcctgca gatcgcgcag ggccgaccag
     5101 agttcctcgc gcgtgaacgc gtcgagcgcg gcgaaaggct cgtcgagcag caggatctcc
     5161 ggttcgtgga tcagcgcgcg gcagatcgac gtgcgctgct gcattccgcc ggagagttcc
     5221 cacggatgct tttcggtaaa ccccgcgagg ccgacggagt gcagcaactc tttcgctttc
     5281 ccggaaaact tccgcctacc gaagttcgac cggtagggct ccacgatctc cagcggcagg
     5341 agcacgttct
//
LOCUS       DASTPD010000279        14093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1781855, whole genome shotgun sequence.
ACCESSION   DASTPD010000279 DASTPD010000000
VERSION     DASTPD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781855"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1781)
                     /locus_tag="VFR57_09230"
     CDS             complement(144..1781)
                     /locus_tag="VFR57_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0259585.1"
                     /translation="MNNFQGLGLSAALVRSVVEQGYTEPTPIQAQAIPVVLAGRDLLG
                     AAQTGTGKTAAFALPILELLGKTTRHAIRVLVVTPTRELAAQVEQDFRTYGKHLDLKT
                     ATIFGGVGMQPQIDALRRGVDILVATPGRLLDHVGEKTVDLSKIEILVLDEADRMLDM
                     GFIHDIRKILALLPTARQNLLFSATFPDEIRKLAGSFMKDPATVEVARRNTPAELVAQ
                     VQHPVAQERKRELLAHLVKSNDWRQVLVFVRTKHGANRLAHQLSRAGIEADAIHGNKN
                     QNHRTRTLKRFKDNELRVLVATDIAARGLDIEALPHVVNYDLPHVAEDYVHRIGRTGR
                     AGAEGEAVSLVAHDDRPLMKAIERLMNRSVEERVITSFEPGTRSQALSSPRPQQQQQP
                     RHRQPKQQRQGQQRRPEQRHGQPKRYGHERPNYATSAYEDQIREARARLVEEGEPLPQ
                     AAAQPQRRPNAGNSAGRPDGGHRSRRGGRGLGGRPDQRNRQARDSRRDTTYSEPRDYT
                     QPHAQPARPVQVTHKPKRTLVGFVGSLLGRKPKPDES"
     gene            complement(2054..2518)
                     /locus_tag="VFR57_09235"
     CDS             complement(2054..2518)
                     /locus_tag="VFR57_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259586.1"
                     /translation="MALFGRRISADDQAIARYDYMLRTAPPATIEQAHAEAFAKLTPE
                     QRRKVLEEMGDRIPAAERAQVERSGTTPEALARAATRAEMRQPGSLGGMMAGSLLSGI
                     AGAVIGSMIAQHFFSNSGADVAEPAQESGVSDSDQATGDSAGFDDMGGGFDV"
     gene            complement(2595..2987)
                     /locus_tag="VFR57_09240"
     CDS             complement(2595..2987)
                     /locus_tag="VFR57_09240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0259587.1"
                     /translation="MEIEFLNFPEAPPPPPTAAYSHAVRAGDFLFVTGQLGIDPKTAR
                     VVQGGVAAEMRQVMKNLEVVLKGAGSSFEKVAMARIFLVHFADDYPIVNRIYAEYFAE
                     GRYPARTTVGVTALAMGCLVEVDLIARL"
     gene            complement(3003..3662)
                     /locus_tag="VFR57_09245"
     CDS             complement(3003..3662)
                     /locus_tag="VFR57_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259588.1"
                     /translation="MPKYRSLAAVIAAALLAFPLYTVAHDRGPAERLVEKYDRFAGSE
                     QNARELVTGLRNDAEIELTRRGKTTEFTPATGKMGWGNVDHALALAKASLADQGIHKP
                     TPEQIKAALNGGTITTKSGEQVKLDGVLKMRASGMGWGEIAHKLGFKLGELKRPIANH
                     HKPDRGHGWEHARGHDWKHERGDWKRDHRPEFHLAKFERPDKPGRPERPERPERHGRG
                     R"
     gene            complement(3749..4630)
                     /locus_tag="VFR57_09250"
     CDS             complement(3749..4630)
                     /locus_tag="VFR57_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0259589.1"
                     /translation="MTRAAAVALMVAAPVLWSSAGVVTRHIQSAQPFEQVFWRSLFAF
                     VFVFVLLLWKKAHPWTAVRKAGTPGLVSGFMWALMFTAFLFALSMTTTANALVVMSVS
                     PLLTAVLASLVLKDPVPLRTWIAAGVAAVGIAWMFSNQVSAHIAGMLIAFLIPVAAAI
                     NVVVLRASAAKLDLVPAVMLGGALSCLIALPFALPFLGTPRDLALLAFLGFFQLALPC
                     VLLVIASRTLLAPEIALLGLLEVVLGPLWAWLGAGEDIGAATFLGGSVVLAALAANEL
                     AALFSPAPGKPSATRVS"
     gene            complement(4627..9951)
                     /gene="uvrA"
                     /locus_tag="VFR57_09255"
     CDS             complement(4627..9951)
                     /gene="uvrA"
                     /locus_tag="VFR57_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0259590.1"
                     /translation="MKHEQVHGDAIVIRGARQNNLKHLNLAIPTGELVVVTGVSGSGK
                     SSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAVEGIPPAIAIDQTNPVRTSR
                     STVGTMTELNDHLKLLFARGAKLYCRGCGKPVVRDTPTSIYEALRVRTDERLIITFPV
                     PIPKNFNEKEVEDLLQRQGYTRLHSRSKTLLEVVQDRVRISEADRSRICEALESALRF
                     GQGKVNIYPDSGVLRFSSDLHCADCDIHYADPTPAMFSFNSPLGACEACRGFGRVIGV
                     DFGLVVPDETKTLRDAIRPWQTASFKECQDDLDKYAKKRKIALDVPWNQLDQQTRQWV
                     LEGEPEWVSWRKSWPGVWYGVRRFFKWLETKAYKMHIRVLLSKYRAYTECVACLGTRL
                     KPEGLLWKIAGKSIHDLMLQPISQTRAFFDALKYEDEATDLLLKEIRTRLGYLCDVGL
                     GYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIEVMKR
                     LRDAGNTLVVVEHDPQIMFAADRILDMGPGPGERGGEITFFGPPEQLRNHSSLTADYL
                     SGRKAAAEPREPIKPREFIEILGAGEHNLKDIDVRIPLGRLVCVTGVSGSGKSTLIQD
                     VLYPALLKTKGKPTEAPGKHRALRGTAKISSVVMVDQSPIGKTTRSNPASYVGAFDVI
                     RALFAKAPVSKERGYTPGTFSFNSGNGRCPTCSGCGFEHVEMQFLSDVYLRCPDCDGK
                     RYRDEILEVRIDGRSIADVLEMTVAEATRFFRKSEEVQARLQPLVDVGLEYLRLGQPV
                     PTLSGGEAQRLKLAGHLARTDESTEKLFLFDEPTTGLHFDDVAKLLRAFRQLLAAGHS
                     LVVIEHNLDVIRAADWIMDLGPEGGDAGGHLVCEGFPKDIKNHPASHTGKALREYEEK
                     MGSDPFSSEVREPRARYLGTAIRIHNAREHNLKNIDVEIPRGRFTVITGVSGSGKSTL
                     AFDILFNEGQRRYLESLNAYARQFVQAASRPDVDAIFGIPPTVAIEQRTSRGGRKSTV
                     GTLTEVHHFLRLLFVKLGTQYCPSCDVPIEPQSEDVIASRLLKGFRNQKITLLAPLVV
                     NRKGVYTDLAKWALAKGYWHLRVDGKMLPTRPFPRIDRFKEHTIELPAGDIVVSAKAE
                     TPLRQLLSKSLEIGKGVVHVLAGKEVQVFSTRRACRSCGRSFPELDPRLFSYNSKHGW
                     CRACYGTGLAIDDVEWTEERARTGAEDNVLDSWIEWLEIDEPCPECDGQRLNPEALAV
                     RWQGRNIAEYASQPIDSLRALFRTVSLKDREREIARDMVAELNARLGFLGEVGLGYLT
                     LDRSAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHHRDNQILLDVLEKLEAK
                     GNSLVVVEHDEDTIRRAQHVVDLGPGAGKLGGEVVAQGAAEELMQNPASLTGRFLLQP
                     LKHPLQPRRPVAKKAECIEVQKASLHNLKNIDVRIPLGRLVAVTGVSGSGKSTLALDV
                     LHNSLKEGQPIGCKGVKGKVERALEVDQTPIGKTPRSCPATYVGFWDSIRKLFADTAE
                     GRMRGWTASRFSFNTAGGRCEACEGQGVKKIEMSFLPDVKVTCEACGGARFNPETLQV
                     RLRGKTAGEVLGMSVDEAVDFFLAHPSIHRCLTLLQDVGLGYLTLGQQSPTLSGGEAQ
                     RIKLVYELSKRSDSKTLYVLDEPTVGLHMADVEKLIRVLHRLVDAGNTAVVIEHNLDV
                     IAEADWIVDLGPEGGDGGGRVVYQGNPENISTGHTANALKAFLAERST"
     gene            10066..11205
                     /locus_tag="VFR57_09260"
     CDS             10066..11205
                     /locus_tag="VFR57_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU0259591.1"
                     /translation="MSRAIKRAAKEKAQLVVIRMDTPGGLDTSMRGLIKDMLASPVPV
                     ATFVAPSGARAASAGTFILYASHIAAMAPGTNVGAASPVSIGGAPAKEDGKKAEPDTM
                     TKKVTNDAVAYIRGLAEMRERNPDWAEKAVREAVSLSAQEALKLNVIDHVAADLPELL
                     KKLGKSDAPMIEVEADWRTKLLGVITNPSIAYILILVGIYALIFEFMNPGLILPGVAG
                     AICLLLALYALHLLPVNYAGLALILLGIAFMVAEAFLPAFGSLGVGGLIAFVIGSIIL
                     MEEATIPGFDIPYPLIAGVTAASAAFLFFIVGMAVRNRKRPVLTGREYLIGAPAEALE
                     DFSAEGWARVQGETWRVRSAAPVRRGQRLRVRSIDGLILTVEVEA"
     gene            11202..11957
                     /locus_tag="VFR57_09265"
     CDS             11202..11957
                     /locus_tag="VFR57_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU0259592.1"
                     /translation="MIQFVLGLVVLGVILLALSIKILREYERAVVFFLGRLQRVFGPG
                     LIFIIPGVQQFVRVDLRIVVFDVPPQDIITRDNVSVKVNAVVYFRVVEPRNAIIQVAN
                     FLEATSQLAQTTLRAVLGKHELDELLSEREKLNLDIQNVLDSQTDAWGIKVSNVEIKH
                     VDLNESMVRAIARQAEAERERRAKVIHAEGELQAAEKLLQAATTLAQRSEAMQLRYLQ
                     TLTQIAGDKASTIVFPVPVDVIGPLLGSKKLPG"
     gene            complement(11938..12174)
                     /locus_tag="VFR57_09270"
     CDS             complement(11938..12174)
                     /locus_tag="VFR57_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259593.1"
                     /translation="MENIVLLDEYRKRRKPVRRPAPSSRVDHASDPTGAHYFCTRCDS
                     DEFRLSALGMVHCAHCSALMRNLLVSPGPNREAS"
     gene            12309..12692
                     /locus_tag="VFR57_09275"
     CDS             12309..12692
                     /locus_tag="VFR57_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEU0259594.1"
                     /translation="MTQPQTEFLDLYRAGLKTAADLMKASLQSAERLQNQQLVAIRTA
                     LDQQAKTISELGQARSIDELLALQTKMAGAQWERTMGYWTELCQTQIAQARDWMSEAA
                     QVATAGSNASMRQVQSGGKQERKAA"
     gene            complement(12741..13604)
                     /locus_tag="VFR57_09280"
     CDS             complement(12741..13604)
                     /locus_tag="VFR57_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU0259595.1"
                     /translation="MPELPDVTVYIESLERLLRGKRLTAIRLFNPFLLRTAVPALSEI
                     HGKTVVGFSRVGKRIVFDFDGDLHLVLHLMIAGRLHWEGKKTRSTLALFEFENGTLSL
                     TEAGSKRRASLHLVRGDAALRALDPGGIEVLAADLATFREKLAKENHTLKRALTDPRL
                     FSGIGNAYSDEILHRAKLSPIALTQNLSAAQVETLFCATRDVLKEWIERLRTERFPEK
                     VTAFRDGMAVHGRYGKPCPVCGAPVQRIVYAENETNYCARCQTGGRILADRALSRLLK
                     KSWPRSIDELP"
     gene            13680..14093
                     /locus_tag="VFR57_09285"
                     /pseudo
     CDS             13680..14093
                     /locus_tag="VFR57_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
BASE COUNT         2338 a         4488 c         4728 g         2539 t
ORIGIN      
        1 gagtccacga tccgttcagc gatcgccgcc ggcgggagcc ttgccagcat ccagaaggac
       61 ttgaggtgcg ggactagttg cggatcctgc gtgtccgaac ttgtgcgcct cgctgcctag
      121 ggtagggcgt cgtcccgctt cggtcagctc tcgtccggct tcggcttgcg gcctagcagg
      181 gaaccgacga aaccgacgag cgtgcgtttc ggcttgtgcg tgacctgcac ggggcgcgcg
      241 ggttgcgcat gcggttgcgt gtaatcgcgc ggctcggagt aggtagtatc gcggcgcgaa
      301 tctcgcgctt ggcgattcct ctggtcggga cgtccgccga gtccgcgccc tccgcgacgg
      361 ctgcgatgac cgccgtccgg cctgccggct gagttaccgg cgttcgggcg gcgttgcggc
      421 tgcgccgcgg cctggggaag cggctcccct tcctcgacca agcgtgcgcg tgcttcgcgt
      481 atctggtctt cgtacgctga ggtcgcatag tttggccgct cgtgaccgta acgtttgggc
      541 tgaccgtggc gctgctcggg gcggcgctgc tgaccttgcc gctgctgctt cggctggcga
      601 tgccgcggct gttgctgctg ctgcgggcga ggtgaactca gagcctggct gcgcgtgccg
      661 ggctcgaagc tggtgatcac gcgctcttcc accgagcggt tcatcaatct ttcgatcgct
      721 ttcatgagcg ggcggtcgtc gtgcgcgacg agcgagacgg cttcgccttc ggcgccggcg
      781 cggccggtgc ggccgatgcg gtgcacatag tcttcggcga cgtgcggaag gtcatagttg
      841 acgacgtgag gcagggcttc tatgtcgagc ccacgcgcag caatgtcggt ggcgacaagc
      901 acgcgcagct cgttgtcctt gaagcgcttg agcgtccttg tgcggtggtt ctggttcttg
      961 ttgccgtgga tcgcgtccgc ctcgatgccg gcgcgcgaga gctggtgcgc gaggcggtta
     1021 gcgccgtgct tggtgcgcac gaagacgagt acctgacgcc aatcgttcga cttcaccagg
     1081 tgcgcaagga gctcacgctt tctttcctgc gcgaccgggt gctggacctg tgccacgagc
     1141 tcggcgggtg tgttgcgtcg cgccacttcg acggtcgccg ggtccttcat gaaggacccg
     1201 gcgagcttgc ggatttcatc ggggaacgtc gccgaaaaga gaaggttctg ccgggccgtc
     1261 ggcaggagcg cgaggatctt gcgaatgtcg tggatgaagc ccatgtcgag catgcgatcg
     1321 gcttcgtcga gcacgaggat ctcgatcttc gacaggtcta cagtcttctc cccgacatgg
     1381 tcgagcaggc ggccgggcgt ggcgaccagg atgtcgacgc cgcggcgcag cgcatcgatc
     1441 tgcggctgca tgccgacgcc gccgaagatc gtggcggttt tgaggtcgag atgttttccg
     1501 taggtgcgga agtcctgctc gacctgcgcg gcgagttccc gggtcggggt tacgacgagg
     1561 acgcggatcg cgtgacgcgt cgtctttcct agaagctcga gaatcggtaa agcgaaagcg
     1621 gcggtcttgc cggtgccggt ctgcgcggca cccaggaggt cgcgcccggc gaggaccacg
     1681 gggatggcct gcgcttggat gggggtcggc tccgtgtagc cctgttccac gacggaacgt
     1741 acaagcgcgg cggacaggcc gaggccttgg aaattattca agtcggtgtt tctcctgcac
     1801 tcggcctggg aagggggggc cttcgaccgg ttcaggcaga tgcttttcta ggctgttgtt
     1861 gagcggtcca ggatggaccg cctagaacga ctgagacgct aaccggatat acgcgtaaca
     1921 gtaacctagg gcgaattata caggccgttc gaccgcggct ctgagcggct gactgccgga
     1981 aactccattc ttaggtttgc gggccatggc ggcatagaaa ccgtcaggaa tgtcgttgaa
     2041 ggggtgagag cttctacacg tcgaaaccgc cccccatatc gtcgaagccc gcgctgtcgc
     2101 ctgtggcctg atccgagtcg gagacccccg attcctgcgc cggttcggcg acatccgcac
     2161 cgctattgga gaagaagtgc tgcgcgatca tgctgccgat gaccgccccg gcgatgccgg
     2221 atagcaggct gccggccatc atgccgccga ggcttccggg ctggcgcatt tccgcccgtg
     2281 tggccgcgcg cgcgagcgct tcgggggtcg tgccgcttct ttccacctga gcgcgctcag
     2341 ctgcagggat tcggtcgccc atttcttcga gcactttgcg gcgctgctcc ggggtgagct
     2401 tcgcgaacgc ctcggcgtga gcctgctcga tggtcgccgg cggcgcggtc ctcagcatgt
     2461 agtcgtagcg ggcgatcgcc tggtcgtccg ctgaaatcct gcgtccaaaa agtgccatgg
     2521 tgtctgctcc tttcgccagc ttagacgcga ccggtgggtg ctacgttcac gccctttacc
     2581 ccatgaaggt cgaatcacag tcgagcgatc aaatccacct ccacgaggca ccccatggct
     2641 agggccgtga cgccgacggt agtgcgggct gggtagcggc cctccgcgaa gtactcggcg
     2701 tagatccggt tcacgatcgg gtaatcgtcc gcgaagtgca ccaggaatat gcgcgccatc
     2761 gcgactttct cgaagctcga gccggcgccc ttcaacacca cctccaggtt cttcatcacc
     2821 tgacgcatct cggcggcgac gccgccttgc acgaccctcg ccgtcttcgg gtcgatgccg
     2881 agctgaccag tgacgaagag gaagtctccg gcgcgtaccg cgtggctgta ggccgccgtg
     2941 ggcggcggcg gaggcgcttc ggggaagtta aggaattcga tctccataac tcccctcgtc
     3001 gctcatcggc cgcgaccgtg cctttccggg cgttcgggac gctcggggcg tccgggctta
     3061 tccggccgct cgaacttcgc aagatggaac tccggacggt gatcgcgctt ccagtcgccg
     3121 cgctcgtgct tccagtcatg cccgcgcgcg tgctcccaac cgtggccgcg atcgggctta
     3181 tggtgattcg cgatgggtct cttgagctca ccgagcttga acccgagttt gtgggcaatc
     3241 tctccccaac ccatgccgct tgcgcgcatc ttgagcacgc cgtcaagctt cacttgctcg
     3301 cccgatttcg tggtgatggt gccgccgttg agcgcggcct tgatctgctc cggcgtcggt
     3361 ttatgaatgc cctgatcggc gagtgaagcc ttcgccaagg cgagcgcgtg atcgacgttg
     3421 ccccagccca tcttgccggt ggccggcgtg aactcggtgg tcttgccccg cctggtgagc
     3481 tcaatttcgg cgtcgttgcg cagtcccgtc accagctcgc gtgcgttctg ctcagatccg
     3541 gcgaaccggt cgtacttctc gaccagccgc tccgctgggc cgcggtcgtg cgctacggtg
     3601 tagagcggga aggcgagcag cgccgcggcg ataacggcgg cgagggatcg atatttcggc
     3661 atgttggcct ccttcgaagc ttcgatgcta ggaccgctga gcgcgaggcc gcaagggggc
     3721 gttcgcattg gtaacaagtc cgttaggact acgaaacgcg cgtcgcagag ggcttgccgg
     3781 gcgccgggct gaatagggcg gcgagctcgt tcgcggccag ggcggcgagc acgacggagc
     3841 cgccgaggaa cgtcgccgcg ccgatatcct ccccggcgcc gagccaggcc cagagcggac
     3901 ccagcacgac ctcgagcagt ccgaggaggg cgatctcggg cgcaagcagc gtgcggcttg
     3961 cgatgacgag gagcacgcaa ggcaaggcga gctggaagaa tccaaggaac gcgagcagcg
     4021 caaggtcgcg tggtgtcccc aagaagggaa gcgcgaaggg cagggcgatc aggcaggaaa
     4081 gcgcgccgcc gagcattacc gccggtacca ggtccagctt cgcggcgctc gcgcgcagaa
     4141 ccaccacgtt gattgccgca gcgacgggga tgaggaaagc gatcagcatt ccggcaatgt
     4201 gcgcggacac ctggttactg aacatccatg cgatgccgac cgcagcaacg ccggcggcga
     4261 tccaggtgcg aagcggcacg gggtccttga gcaccaggct tgcgaggacg gcggtgagga
     4321 gcgggctcac gctcatgacc acgagcgcgt tcgccgtggt cgtcatggaa agcgcaaaca
     4381 ggaacgcggt gaacatcagc gcccacatga aacccgatac gagcccgggc gtgccggcct
     4441 tgcgcacggc cgtccacgga tgggctttct tccacaacag gaggacaaac acgaatacga
     4501 aggcgaacag gctgcgccag aacacctgct cgaacggctg cgcactttgt atgtggcgcg
     4561 ttaccacgcc ggcgctcgac cagagcaccg gcgccgcaac catcagcgcg acagcggcgg
     4621 cgcgcgtcaa gtgcttcttt ccgcgagaaa cgccttgagc gcgttcgcag tgtggccggt
     4681 cgagatattt tcggggttgc cctgatagac gacgcggccg ccgccgtcac ccccctcggg
     4741 tccgagatcg acgatccagt cggcttcggc gattacatcg aggttgtgct cgatcaccac
     4801 cgcagtgttg cccgcgtcga ccaggcgatg cagcacgcgg atcagcttct cgacgtcagc
     4861 catgtggaga ccaacggttg gttcgtcgag gacgtagagc gtcttgctgt cgctccgctt
     4921 cgagagctca tagaccagct tgatgcgctg agcttcgccc ccggacagcg tcgggctttg
     4981 ctggccgagc gtgaggtagc cgaggccgac gtcctgcaag agcgtgaggc agcggtgaat
     5041 ggaagggtgc gcgaggaaga aatcgaccgc ttcgtccacg ctcatgccga gcacctcgcc
     5101 cgccgttttg ccgcgcaagc gcacctgcag agtctcggga ttgaagcgcg cgccgccgca
     5161 ggcttcgcat gtgaccttca catccggcag gaacgacatc tcgatcttct tcacgccctg
     5221 gccctcgcac gcctcgcagc gaccgccggc cgtgttgaac gagaagcggc tcgcggtcca
     5281 gccgcgcatg cgcccctcgg ccgtatcggc gaacagcttg cggatcgaat cccagaagcc
     5341 gacataggtc gcgggacacg agcgcggcgt cttgccgatc ggagtctggt cgacctcgag
     5401 cgcgcgctcg actttcccct tcacgccctt gcagccgatc ggctggccct ccttgaggct
     5461 gttgtggagc acatcgagag cgagcgtgga tttgcccgac cccgagacgc cggtgacggc
     5521 gaccagccgg ccgagcggaa tgcgcacgtc gatgttcttc aggttgtgca ggctcgcttt
     5581 ctgcacttcg atgcactcgg ccttcttcgc caccggccgg cgtggctgaa ggggatgctt
     5641 caaaggctgc aacaagaacc ggccggtgag cgaggccggg ttctgcatca gctcctcggc
     5701 cgcaccttgc gcgaccacct cgccgccgag cttgcccgcg ccggggccga gatcgacgac
     5761 atgctgcgcg cggcggatcg tgtcttcgtc gtgctcgacg acgacgagcg agttgccttt
     5821 cgcctcgagc ttctccagca cgtcgagcag gatctggtta tcgcgatgat gcaggccgat
     5881 cgtcggctcg tcgaggatgt agcagacgcc gcgcaaattg cttcccaatt gggcggccaa
     5941 tctgattcgc tgcgcttcgc cacccgagag cgtcggtgcc gagcggtcga gcgtgaggta
     6001 gccgaggccc acttcaccga gaaagcccag ccgcgcgttc agctcggcaa ccatgtcgcg
     6061 cgcgatctcg cgttcccggt ccttcaagga caccgttctg aacagcgcac gcagcgaatc
     6121 gatcggttgt gaagcgtact cggcgatatt cctgccttgc cagcgtaccg cgagcgcttc
     6181 ggggttcagg cgctgtccgt cgcactcggg gcacggctca tcgatctcca gccattcgat
     6241 ccaggagtcg agcacgttgt cctcggcgcc agtgcgcgcg cgctcttcgg tccattccac
     6301 gtcgtcgatg gcgaggccgg tgccgtagca cgcccggcac cagccgtgct tggagttgta
     6361 cgagaagagc cgcggatcga gctccgggaa gctgcggccg caggagcggc acgcgcgtct
     6421 ggtcgagaac acctggacct ccttgcccgc gagcacgtgc acgacgcctt tgccgatctc
     6481 gagagacttg gacagcaact gccgcaacgg ggtctccgcc ttcgcggaga cgacgatatc
     6541 ccctgcgggc agctcgatgg tgtgctcttt gaagcggtcg atgcgcggga agggccgcgt
     6601 cggcagcatc ttgccgtcga cgcgcaggtg ccaatagccc ttcgccaacg cccacttcgc
     6661 cagatcggtg tagacgccct tgcggttcac caccaacgga gcgagcagcg tgatcttctg
     6721 gttgcgaaag cccttcaaga ggcgcgaagc gatgacgtcc tcgctctgcg gctcgatcgg
     6781 cacgtcgcag ctcgggcagt actgcgtgcc gagcttcaca aagagaaggc gcaggaagtg
     6841 gtgcacctcg gtcagcgtgc cgacggtcga ctttcgtccg ccgcggctcg tgcgctgctc
     6901 gatcgccacc gtcggtggga ttccgaaaat cgcatcgacg tcgggccgcg acgccgcctg
     6961 cacgaactga cgcgcgtacg cgttcaggga ttcgagataa cgtcgctgcc cttcgttgaa
     7021 caggatatcg aaggcgagcg ttgactttcc cgatcccgag acgccggtga tcaccgtgaa
     7081 gcgcccgcgc gggatttcca cgtcgatgtt cttcaggttg tgctcgcgcg cgttgtgaat
     7141 gcggatcgca gtgccgaggt agcgtgcgcg cggctcgcgc acctcggaag aaaaggggtc
     7201 tgaccccatt ttttcttcgt actcgcgcag cgctttgccc gtgtgcgagg ccggatgatt
     7261 cttaatgtct ttcggaaaac cctcacagac gaggtgaccg ccggcgtcac cgccttccgg
     7321 gccgagatcc atgatccagt cggcggcgcg gatcacgtcc aggttgtgct cgatgacgac
     7381 gagtgaatgt cccgcggcga gaagctggcg gaacgcgcgc aaaagcttcg cgacgtcgtc
     7441 gaaatgcagg cccgtggtcg gctcgtcgaa aaggaagagc ttctccgtcg actcgtcggt
     7501 cctggccaga tggccggcga gcttgaggcg ctgcgcttcg ccgcccgaga gcgtgggcac
     7561 gggctggccg agacgcaggt attcgaggcc gacgtcgacg agcggctgca ggcgcgcctg
     7621 cacttcctcg ctcttgcgaa agaagcgcgt cgcttcggcg accgtcatct cgagcacatc
     7681 ggcgatcgat cttccgtcga tgcgcacctc gaggatctcg tcgcgatagc gcttgccgtc
     7741 gcagtcgggg cagcgcaggt acacgtcgga gaggaactgc atctcgacat gctcgaagcc
     7801 gcaacccgag caggtggggc agcggccgtt gccggagttg aagctgaaag tgcccggcgt
     7861 atagccgcgt tccttagaga ccggcgcttt agcgaacaag gcgcggatga catcgaaggc
     7921 gccgacgtag ctcgcgggat tggagcgcgt ggtcttgccg atgggcgact ggtcgaccat
     7981 caccacgctc gagatcttcg ccgtgccgcg cagcgcgcgg tgcttgcccg gcgcctcggt
     8041 cggcttgccc ttcgtcttga ggagcgccgg gtagagcacg tcctggatga gggtcgactt
     8101 gccggagccc gagaccccgg tgacgcacac gaggcggccg agcgggatgc ggacgtcgat
     8161 gtccttcagg ttgtgctcgc ctgcgccgag gatttcgatg aactcgcgcg gcttgatcgg
     8221 ctcgcgcggc tcggcggccg ccttgcgccc cgagaggtaa tcggcggtca gcgacgaatg
     8281 gttcctgagc tgctcgggcg gcccgaagaa cgtgatctcg ccaccgcgct cacccggtcc
     8341 gggtcccatg tcgaggatgc gatcggcggc gaacatgatc tgtggatcgt gctcgactac
     8401 gacgagcgtg ttgccggcgt cgcgcaggcg cttcatcacc tcgatcacgc gtcccatgtc
     8461 gcgcggatgc aggccgatcg acggctcgtc gagcacgaag agtgtgttga cgagcgaggt
     8521 gccgagcgcg gtggtgaggt tgatgcgctg tacttcgccg ccggagagcg tgcgcgactg
     8581 gcgatccagc gtcagatagc ccaggccgac gtcgcagagg tagccgagcc gcgtgcggat
     8641 ctccttcagg aggaggtcgg tcgcttcgtc ctcgtacttc agggcatcga agaacgcgcg
     8701 cgtttgcgag atcggctgga gcatcagatc gtggatcgac ttgccggcga tcttccaaag
     8761 cagcccctcc ggcttgagcc gcgtgccgag gcacgcgacg cactcggtgt acgcccggta
     8821 cttcgagagc agcacgcgga tgtgcatctt gtacgccttg gtctcgagcc acttgaaaaa
     8881 gcggcgcacg ccgtaccaga cgccgggcca ggacttgcgc cagctcaccc attccggctc
     8941 gccttcgagc acccactgcc tggtttgttg gtcgagctgg ttccagggaa cgtcgagcgc
     9001 gatctttcgc ttcttcgcgt acttgtcgag atcgtcctgg cactccttga agctcgctgt
     9061 ctgccacgga cggatcgcat ctcgcagcgt cttcgtctcg tcgggcacca ccagaccgaa
     9121 atcgacgccg atcacgcggc cgaacccgcg gcatgcctcg cacgcgccga gcggcgagtt
     9181 gaaggagaac atcgccggcg tcggatcggc gtagtggatg tcgcaatccg cgcaatgcag
     9241 gtcggacgaa aaccgcaaca ccccagaatc ggggtagatg ttgaccttgc cctgcccgaa
     9301 ccgcagggca gactcaagcg cctcgcagat ccgcgagcgg tcggcctccg agatgcgcac
     9361 gcggtcctgc acgacctcga ggagcgtctt cgagcgcgag tgcaggcgcg tgtagccctg
     9421 ccgctgcagc aggtcttcga cttccttctc gttgaagttc ttcgggatcg ggaccgggaa
     9481 ggtgatgatg aggcgctcgt cggtccggac cctgagcgct tcatatatgg aagtcggggt
     9541 atcgcgcacg accggcttgc cgcagccgcg gcagtagagc ttcgccccgc gcgcgaaaag
     9601 gagcttcagg tggtcgttca gctcggtcat cgtgccgacc gtggaacgcg aagtgcgcac
     9661 cgggttggtc tggtcgatgg cgatggcggg cgggatgccc tcgaccgcgt cgacctgcgg
     9721 cttgtccatg cggtccagga actggcgcgc gtaggggctg aaggtctcga cgtaacggcg
     9781 ctggccttcg gcatacaaag tgtcgaagac gagcgacgat ttccccgaac ccgaaacgcc
     9841 ggtcacgacg accagctcgc cggtcgggat cgctaggttg aggtgcttga ggttgttctg
     9901 gcgggcgccg cggatgacaa tggcgtcgcc gtggacttgc tcatgcttca ccggggcgca
     9961 ttttatctgc tggcgaccct gatttggggc gcggcacgcg cgcagcccgc gcccgtgttg
    10021 gtcgtgccgc ttgacggggc gatcggaccg gcatccgcgg acttcgtgag ccgcgcgatc
    10081 aagcgcgccg cgaaggagaa ggcgcagctc gtcgtcatcc gcatggacac gcccggcggg
    10141 ctcgacactt ccatgcgggg cctgatcaag gacatgctcg cttcgcctgt tcccgtcgcc
    10201 acgttcgtcg cgccgagcgg cgcgcgcgcc gcgagcgccg gcacgttcat tctgtatgca
    10261 agccacatcg ccgctatggc gccgggaacg aacgtcggcg cggcctcacc cgtgtcgatc
    10321 ggcggcgcgc cggcgaagga ggacggaaag aaggccgagc ccgacacgat gacgaagaag
    10381 gtcacgaacg acgcggtggc ctacatccgc ggcctcgccg agatgcgcga gcgaaacccg
    10441 gactgggccg agaaagcggt gcgcgaagcg gtgagcctct ccgcgcaaga ggcgctgaaa
    10501 ctgaacgtca tcgaccatgt cgccgctgac cttccggagc tcctgaaaaa gctcggcaag
    10561 agcgatgcgc ccatgatcga agtcgaggcc gactggcgca cgaagctgct cggcgtgatc
    10621 accaatccga gcatcgcgta catcctgatc ctcgtcggga tctacgcgct cattttcgag
    10681 ttcatgaacc ccggcctcat cctgccgggg gtggccggcg cgatctgcct gctgctcgcg
    10741 ctttacgcgt tgcacctcct gccggtgaac tacgccgggc tggcgctcat cctgctcggc
    10801 atcgcgttca tggtggccga ggccttcctc ccggccttcg gctctctggg cgtcggcggc
    10861 ctgattgcgt tcgtcatcgg ctcgatcatc ctcatggaag aggcgaccat accgggcttc
    10921 gacatcccgt atccgttgat tgccggcgtg accgcggcga gcgcggcgtt tcttttcttc
    10981 atcgtcggca tggccgtgcg caaccgcaag cgaccggtcc tgaccgggcg cgagtatctg
    11041 atcggcgcgc ccgccgaggc gctggaagac ttttccgccg agggctgggc gcgcgtgcag
    11101 ggcgagacct ggcgcgtgcg ctcggccgcg ccggtgcgcc gcggccagcg cctgcgcgtg
    11161 cgatcgatcg acggcctcat ccttaccgtg gaggtggaag cgtgatccag ttcgttctcg
    11221 gcctcgtcgt gctcggcgtc atcctgctcg cgctgtccat caagatcctg cgcgagtacg
    11281 agcgcgcggt ggtgttcttc ctcggccgct tgcagcgcgt gttcgggcca gggctgatct
    11341 tcatcatccc gggcgtccag cagttcgttc gcgtcgactt gcgcatcgta gtttttgacg
    11401 tgccgccgca ggacatcatt acgcgcgaca acgtttcggt gaaggtgaac gccgtggtgt
    11461 acttccgcgt ggtcgagccg cgcaacgcca tcatccaggt ggcgaacttt ctcgaagcca
    11521 cgagccagct cgcgcagacc acgctgcgcg cggtgcttgg caagcacgag ctcgacgagc
    11581 tcctttcgga gcgcgagaag ctcaatctcg acattcagaa cgtgctcgac agccagaccg
    11641 acgcctgggg catcaaggta tccaacgtcg agatcaagca cgttgacctc aacgaatcga
    11701 tggtgcgcgc gatcgcccgc caggcggaag ccgagcgcga gcgccgcgcc aaagtcattc
    11761 atgccgaagg ggaattgcag gccgccgaga agctcctgca ggccgcgacc acccttgcgc
    11821 agcgctccga ggcgatgcag cttcgctacc tgcagacgct cacgcagatc gccggcgaca
    11881 aggcctcgac catcgtgttc ccggtgccgg tggacgtcat cggcccgttg ctcgggtcta
    11941 agaagcttcc cggttagggc ccgggctcac cagcaggtta cgcatcagcg cgctgcaatg
    12001 ggcgcaatgg accatgccga gcgcggagag gcggaactcg tcggagtcgc aacgcgtgca
    12061 gaagtaatgc gcgcccgtgg gatcgctcgc atggtcgacg cgggacgagg gtgccggccg
    12121 gcgcacgggc ttgcgccgct tgcggtactc gtcgagaagg acgatgtttt ccattaatgg
    12181 cgggtgtgta cgcaatttga ttcttttaat ttgcgcccgg ttcctcgccg cgtatattca
    12241 tttaaagccg ccacgaagcg acggcatgta gcttgggagg gacaactgca ctccacaagg
    12301 agaagcgaat gacccaaccg cagaccgagt ttctcgacct gtaccgcgcg ggcctcaaga
    12361 ccgccgccga cctgatgaag gcgtcgttgc aaagcgccga gcgcctgcaa aaccagcagc
    12421 ttgtcgccat ccgcaccgcg ctcgaccagc aggcgaaaac gatcagcgag ctcggccagg
    12481 cgcgcagcat cgacgagttg cttgcgctgc agaccaagat ggccggcgcg cagtgggagc
    12541 gcacgatggg ctactggacg gagctctgcc agacccagat cgcgcaggcc cgcgactgga
    12601 tgagcgaggc cgcccaggtc gcgaccgccg gtagcaacgc cagcatgaga caggtccaga
    12661 gcggcggcaa gcaggagcgg aaagccgcct aacccgaatt cgggacgtag ccctaaccga
    12721 ctaggactcc gtccccgaat tcaaggcagt tcatcgatcg agcgcggcca gctcttcttg
    12781 agaagccggg acagcgcgcg gtcggcgagg atgcgcccgc cggtctggca gcgcgcgcag
    12841 tagttcgttt cgttctccgc gtacacgatt ctctgcacgg gcgcgccgca caccgggcag
    12901 ggcttgccgt agcggccatg caccgccatc ccgtcgcgga aggccgtgac tttctcggga
    12961 aagcgctcgg tgcgcagccg ctcgatccat tctttcagga catcgcgcgt ggcgcagaac
    13021 agcgtctcga cctgcgccgc ggacaggttt tgcgtgaggg cgatcggcga gagcttcgcg
    13081 cggtgcagga tttcgtcgga gtaggcgttg ccgatgcccg aaaaaagccg cggatcggtg
    13141 agcgcgcgct tgagcgtgtg attttccttg gcgagcttct cgcggaaggt cgctaggtcg
    13201 gccgcgagca cttcgatgcc gccggggtcg agcgcgcgta acgcggcatc gccccgcacg
    13261 aggtgcagcg aggcgcggcg tttcgaaccg gcctccgtca gagaaagcgt gccgttctcg
    13321 aactcgaaca gcgcgagggt gctgcgcgtt ttcttgcctt cccagtgcag gcgtccggcg
    13381 atcatcaggt gcagcacgag gtgcagatcg ccgtcgaagt cgaacacgat gcgttttccg
    13441 actctggaaa atccgacgac ggttttgcca tgaatctcgg acagcgccgg gaccgcggtg
    13501 cgcagcagaa acgggttgaa gaggcggatc gccgtcagcc gcttgccgcg cagcaggcgt
    13561 tcgaggcttt cgatgtagac cgtgacgtcg ggaagctccg gcatgcctcg atgataagat
    13621 cggcgtgcat gtcgttactg cagacgcggt tgcgcagcgg gtaattcgtc gtcaccgcgg
    13681 agatcacgcc gcccgtctcc accgaccctg cggagttcct gcgccgcgcg ctgccgctca
    13741 agggcctcgc cactgcgatc aacgtgacgg acggggcggg cgccaaggtg catctgtcga
    13801 gcctcgccgc ggcgcacttc ctcgtgcaga gcggcctcga gcccatcttt cagatgacct
    13861 gccgcgaccg aaaccgcatc gcgctgcagg gcgacctgct cggcgcggcg gccttgggcg
    13921 tgcacaacat cctggtcctc gcgggcgacg atcccaaggt cggcgaccag cccgaagcca
    13981 aggcggtcta cgacgtcgac agccgcggcc tgatggcgat ggcgaaatcc atgcgcgacc
    14041 agcgccggct gccgtcgatc agcgtgcccg aagggagctt cccctccttc ctc
//
LOCUS       DASTPD010000280        10351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_354282, whole genome shotgun sequence.
ACCESSION   DASTPD010000280 DASTPD010000000
VERSION     DASTPD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354282"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VFR57_09290"
     CDS             complement(<1..1845)
                     /locus_tag="VFR57_09290"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0259596.1"
                     /translation="MEKSFNPAAIEQPWYQRWESAGYFGHGGDATPAYCIQLPPPNVT
                     GTLHMGHAFQQTLMDALIRYHRMRGYNTNWVVGTDHAGIATQIVVERQLQEEGKTRHD
                     LGREKFVERVWAWKQQSGSTITRQMRRLGASANWAYADTGAQRAGYFTMDAKMSRAVV
                     EVFVRLYEEGLIYRGKRLVNWDPLLGTAVSDLEVDTEEEDGRIWEIRYPGEKFSITVA
                     TVRPETMLGDVAVAVHPKDPRYAGLVGKRVALPLTGRSIPIIADDYVDPQFGTGAVKI
                     TPGHDFNDYHVGKRHSLEPISVLKLDAHLNDNAPAKYQGMERFEARKRMLADLKAEGL
                     LVSEKPYKLRVPRSGRTGVIVEPMLTDQWFVAMEGLARKGLEAVANGEVRFFPEHWTK
                     TYNEWLGNIQDWCISRQLWWGHQIPAWYDADGEIYVARTEDEARQKAGGKPLRRDPDV
                     LDTWFSSALVPFSSLGWPDKTKDQEVFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKV
                     PFRHVYINAIVRDAEGEKMSKSKGNTLDPLDLIDGITLDALLKKSTVGLMKADHRQKI
                     ERYVRTHFPNGIPPFGADALRFTFAALASFARTLNFDLSRCEGYRNFCN"
     gene            complement(1848..2102)
                     /locus_tag="VFR57_09295"
     CDS             complement(1848..2102)
                     /locus_tag="VFR57_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259597.1"
                     /translation="MTDDVPVLTEVVSEEPAARAPDPIALEALAREIERAVLQRLGPE
                     VDRVMDQALDAARAELKASVTRIVREALAASLAEASRTPE"
     gene            complement(2095..2517)
                     /locus_tag="VFR57_09300"
     CDS             complement(2095..2517)
                     /locus_tag="VFR57_09300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEU0259598.1"
                     /translation="MTSIDFYFNAPDRLQVACRLAGKAMKLNQRVLIYAPQAELAARI
                     DKMLWTWPATGFVPHCAVHAPLARETPVLIASGDETPEGCEILLNLDANCPPHFERFP
                     RLLEVTDEAGREAGRNRYRFYKERGYRIANHDLAQAND"
     gene            complement(2514..3989)
                     /locus_tag="VFR57_09305"
     CDS             complement(2514..3989)
                     /locus_tag="VFR57_09305"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU0259599.1"
                     /translation="MEFSIKTLSPESAKTGCVVVGVHTAGELTAAARRIDQAAKGALK
                     KAARDISGKTGSTLLLHSLPGVAAERVLLVGLGDKSTKEAAYRDAVRAAANALKELDA
                     KDAALFLVDMKIGSRPVSWNVRHAVLAMQDALYRFDQLKTQNKANGRALAAVTLPLSP
                     KAELQHALREAVATSEGSALARTLGNLPPNICTPAYLADEAKKLAREFKLELEVLERK
                     DMEKLGMGALLAVARGSQQPPKLIVLRYGAGAKSRKSLAFVGKGITFDTGGISLKPAG
                     EMDEMKFDMSGAASVLGALRALAAMKAPVNVVGVIPACENMPGGAATKPGDVVTTMSG
                     QTVEILNTDAEGRLILCDALSYAERFKPDAVVDIATLTGACVIALGHVATGLFANDQK
                     LADEIRAAADDAWDRVWQLPLWEDYQEQLRSNFADFANIGGRPAGSITAACFLARFTR
                     NLRWAHLDIAGTAWKSGRDKGSTGRPVPLLVRFALRHAGRA"
     gene            4006..5082
                     /gene="lptF"
                     /locus_tag="VFR57_09310"
     CDS             4006..5082
                     /gene="lptF"
                     /locus_tag="VFR57_09310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU0259600.1"
                     /translation="MIFQRALLREFAGLAGAVFMTLFAIAVTTRLIRLLGQAAGGKFP
                     SDAVFAFLGFFALGALPVLLSLTMFISVLLTMTRSWRDSEMVIWFASGQPLTAWLRPV
                     MLFALPQIAVIGALSLFISPWAAQMAGQYTSKIEQRDDVSRVTPGVFGETANRTRVFF
                     VESVSGDSSAVQNVFVSSVHGTKSGVSMSRSGHTETAPNGDRFIVLEQGRRYEGAPGD
                     PEYRVTEFDRYAARIELKEGREVTPTHKNLPTLALISNPTNQNLGELLWRIGIPISAF
                     VLVLLAIPMSFVNPRAGRSANLLFALLTYIVYSNLLSVSQARVAQGRMDFGVGWWLVH
                     AGMVVLLVIMFAQRMQLIRLRIGR"
     gene            5097..6170
                     /gene="lptG"
                     /locus_tag="VFR57_09315"
     CDS             5097..6170
                     /gene="lptG"
                     /locus_tag="VFR57_09315"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU0259601.1"
                     /translation="MRTLERYLARQIYGATAFVLLGFLGLFAFFDLIRELADLGKVEY
                     HLPQVFSFVLLSMPTHAYELFPVVVLIGTLYVLALLASNSEYTVMRASGLSPRRAGLA
                     LAKVGIPFVVLTFVIGEWVAPWCEEQAQKVKMRAMSSMIGQDLTSGLWFKDEGAFINV
                     KEARQTNLLNGVRIYDFDADYRLQRITLAQRAEYAGKGNWRLTEVMRTHFTTEGPRIE
                     KQPELEWRSRVTPDMLDALIVRPERMSAWALHKYTEHLAGNRQRTERYEIALWKKLFY
                     PLAALVMMALALPFAYMQARAGMVGVKVFLGIMLGIFFHMLNSLFSHIGLLQNWPPLS
                     AAIVPSLAFLMAAIMMMWWVERR"
     gene            complement(6167..6559)
                     /locus_tag="VFR57_09320"
     CDS             complement(6167..6559)
                     /locus_tag="VFR57_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0259602.1"
                     /translation="MSGATPGLARRLASMVYEAVLLFAVAFFAAWLFFFASGGEDTTS
                     GWLRTALQVFIAAFFAGYFFWCWLRGGQTLAMKAWHIRLVGVTPRKAMLRFLLALAFL
                     PASILWALFDRDRQFLHDRLAGTRLVNA"
     gene            complement(6556..7032)
                     /locus_tag="VFR57_09325"
     CDS             complement(6556..7032)
                     /locus_tag="VFR57_09325"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEU0259603.1"
                     /translation="MTRAAWLLVAALLCGGALAEPPVEVQRKAAPAKAKPAAKAKAQV
                     RPAWAELTAEQQQILAPLKADWESLEPERRRKWIGVAKRYPKMQPQAQERVQRRMQAW
                     AKLTPEQRRQARETYKQIAKTPPEKRQNLREQWAEYQQLPPQERENLTAPGERRRR"
     gene            complement(7029..7406)
                     /locus_tag="VFR57_09330"
     CDS             complement(7029..7406)
                     /locus_tag="VFR57_09330"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEU0259604.1"
                     /translation="MNEMQFGHRIRQLLTQGTKVRPDVARRLAAARMRALEHQRAEVA
                     GLAWADNLVGRMGGWSGFALRVLLPIALLVGSGAALYGWQQNRLAAEVEEIDAQLLTD
                     DLPIDAYLDRGFQNWLKKRAADE"
     gene            complement(7403..8023)
                     /locus_tag="VFR57_09335"
     CDS             complement(7403..8023)
                     /locus_tag="VFR57_09335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0259605.1"
                     /translation="MLTCRAKQQRAATLASRSELSQFLEGIEKRAFKQAVFAVRDDEA
                     ALDIVQDAMLRLAEKYGARPAGELPMLFHRIMQNAIRDWYRRQKVRSLWTTLLSSLGP
                     GREDDDEQDPLETMLPEGGSNSQEAPAERFEQAQVLEIIEQEISRLPARQREAFILRY
                     WEELDVAETARAMNCSEGSVKTHCSRAAHALQAALSARGITFETKS"
     gene            8114..9826
                     /locus_tag="VFR57_09340"
     CDS             8114..9826
                     /locus_tag="VFR57_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEU0259606.1"
                     /translation="MDRAKTTTLTGAEIVVRCLQEEGVEYVFGYPGGAVLFIYDELFK
                     QDKVKHVLVRHEQGAVHAADGYSRSSRKVGVALVTSGPGVTNAVTGIATAYMDSIPLV
                     VITGQVPTQAIGLDAFQECDTVGITRPIVKHNFLVKNVADLASTIKKAFHIAATGRPG
                     PVVVDIPKDVTQHACEFHYPDTVHMRSYNPVRKGHAGQIKKAVQLLLDAERPFVYTGG
                     GVVLSDASVELRELVKQLGFPCTNTLMGLGAYPATDRQFLGMLGMHGTYEANMAMQHC
                     DVLLAIGARFDDRVIGNPAHFAQNPRKIVHIDIDPSSISKRVKVDVPIVGNIPDVLED
                     LLKLLKGSRGKDISAWWKQIDGWRAKDCLRYDRKSPIIKPQFVLEKLYEVTRGEAFVT
                     SDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGFGLPAAMGVQLANRGATVACVTGESS
                     IQMCLQELSTCKQYRLPIKIINLNNKYRGMVRQWQEFFHGNRYSESYMDALPDFVKLA
                     EGYGHSGLLVDKPADVEPALRETFKRKNDLVFLDFLTDQAENVYPMVPGGKGITEMIL
                     SEDL"
     gene            9826..10317
                     /gene="ilvN"
                     /locus_tag="VFR57_09345"
     CDS             9826..10317
                     /gene="ilvN"
                     /locus_tag="VFR57_09345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009970334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU0259607.1"
                     /translation="MRHIISILVENEAGALSRISNMFSARAYNIESLTVAPTEDASMS
                     RMTVVTSGSDDVIEKITKQLNKLVEVVKVVDLSEAEHIERELMLIKVRAGAKEREDMK
                     RMCDIFRGRVIDVSDNTYTIELTGEGAKLDAFIKALDQGTIIETVRTGASGIGRGNRI
                     LRT"
BASE COUNT         1703 a         3373 c         3389 g         1886 t
ORIGIN      
        1 gttgcagaaa ttgcggtagc cctcgcaccg gctcaggtca aagttgagcg tgcgcgcgaa
       61 gctcgcaagc gccgcgaagg tgaagcgcag cgcgtcggcc ccgaacggcg gaatgccgtt
      121 cgggaaatgc gtgcgcacgt agcgctcgat cttctgcctg tggtcggcct tcatcaggcc
      181 gacggtggac tttttcagga gcgcatccag cgtgatgccg tcgatgagat ccagaggatc
      241 gagcgtgttg cccttcgact tggacatctt ctcgccctcg gcgtcgcgca cgatggcgtt
      301 gatgtacacg tggcggaacg gcacttttcc ggtgaaatgc gtggtcatca tcaccatgcg
      361 cgccacccag aagaagatga tgtcgaagcc ggtgaccagc accgaggagg gcaggaatac
      421 ttcctggtcc ttcgtcttgt ccggccagcc gagcgaggag aacggcacca gcgcggagga
      481 aaaccaggta tcgagcacat cgggatcgcg ccgcaatggt ttgccgcccg ccttctggcg
      541 cgcctcgtct tccgtgcgtg caacgtagat ctcgccgtcg gcgtcgtacc aggccgggat
      601 ctggtggccc caccagagct ggcgcgagat gcaccagtcc tggatgttgc cgagccactc
      661 gttgtaggtc ttggtccagt gctcggggaa gaaccgcact tcgccgttcg ccacggcctc
      721 gaggcctttc ctggcgaggc cttccatggc cacgaaccac tgatcggtga gcatcggctc
      781 gacgatgacc ccggtgcggc cggagcgcgg cacgcgcagc ttgtacggct tttcggagac
      841 gagcaggccc tcggccttga ggtcggcaag cattcgcttc ctcgcctcga agcgctccat
      901 gccttggtac tttgccggcg cgttgtcgtt gagatgcgcg tcgagcttga gaacgctaat
      961 cggctcgagg ctgtgtcgct tgccgacgtg gtagtcgttg aagtcgtggc cgggggtaat
     1021 cttgaccgcg ccggtgccga actgcggatc cacgtagtcg tcggcgatga tagggatgga
     1081 acgaccggtc agcggcagcg cgacgcgttt gccgacgagg ccggcgtagc gcgggtcctt
     1141 cggatgtacc gcgaccgcga cgtcgccgag catcgtctct ggacgcaccg tggcgacggt
     1201 gatgctgaat ttttcccccg gatagcggat ctcccagatc ctgccgtctt cttcctcggt
     1261 gtccacctcg aggtcggaca cggcggtgcc gagcaacggg tcccagttca ccaggcgctt
     1321 gccgcggtag atgagtcctt cttcgtagag ccggacgaac acctccacga cggcgcgcga
     1381 catcttggcg tccatggtga agtagccggc gcgctgagct cccgtatctg cgtaggccca
     1441 gttcgccgag gcgccgaggc gccgcatctg ccgggtaatg gtcgagcccg actgctgctt
     1501 ccacgcccat actcgctcga cgaacttctc gcgtccgagg tcatggcggg tcttgccttc
     1561 ctcctgcagc tggcgctcga ccacgatctg cgtcgcgatg ccggcgtgat cggtgccgac
     1621 gacccagttg gtgttgtagc cgcgcatgcg gtggtagcgg atgagcgcgt ccatgagcgt
     1681 ctgctggaac gcgtggccca tgtgcagcgt gccggtcacg ttgggcgggg gaagctggat
     1741 gcagtacgcc ggcgtggcgt cgccgccatg cccgaagtag cccgcgctct cccagcgctg
     1801 gtaccacggc tgctcgatgg cagcgggatt gaagctcttc tccacggcta ctccggcgtg
     1861 cggctggcct cggcgagcga agccgcgagc gcttcgcgga cgatccgcgt aacgctcgcc
     1921 ttgagctcgg cgcgcgcggc gtcgagcgct tggtccatca cgcggtcgac ctcgggaccg
     1981 aggcgctgga gcaccgcgcg ctcgatctcc cgcgcgagtg cctcgagcgc gatagggtcg
     2041 ggcgcgcgcg ccgcgggctc ctcgctcacg acttccgtga ggacgggtac gtcgtcagtc
     2101 attcgcttgg gcgaggtcgt gattggcgat gcggtagccg cgctccttgt agaagcggta
     2161 gcggttgcgg ccagcctcac ggccggcctc gtcggtcact tcgagaagcc gtggaaagcg
     2221 ttcgaagtgc ggcggacagt tggcgtcgag gttgagcagg atctcgcagc cttccggcgt
     2281 ttcgtcaccg gaggcgatga gcaccggggt ctcgcgcgca agcggcgcgt gcacggcgca
     2341 gtgcggcacg aaacccgtcg ccggccaggt ccacagcatc ttgtcgatgc gcgccgcgag
     2401 ctcggcctgc ggcgcataga tgagcacacg ctggttcagc ttcatcgcct tgccggcgag
     2461 ccggcaagcg acctgcagcc ggtccggcgc attgaaatag aagtcgatgc tggtcatgcg
     2521 cgcccggcgt ggcgcagcgc gaagcgcacg agaagcggca ccgggcgccc ggtcgagccc
     2581 ttgtcgcggc cgctcttcca cgcggtgccg gcgatatcga ggtgggccca gcgcagattg
     2641 cgtgtgaaac gcgcgagaaa gcaggcggcg gtgatgctgc cggcgggacg cccgccgata
     2701 ttcgcgaaat cggcgaaatt cgagcgcagc tgctcctggt agtcctccca gagcggcagc
     2761 tgccagacgc ggtcccaggc gtcgtctgcc gcggcgcgaa tttcgtcggc gagcttctgg
     2821 tcgttggcga atagtccggt ggcgacgtgt ccgagcgcga tcacgcacgc gccggtgagc
     2881 gtggcgatgt ccacaacggc gtcgggcttg aagcgctcgg cgtacgagag cgcatcgcac
     2941 aagataagcc gcccttcggc atcggtgttc aggatctcga cagtttggcc cgacatggtg
     3001 gtgacgacgt cgccgggctt ggtggcggcg ccgccgggca tgttctcgca ggccgggatg
     3061 acgccgacca cgttgaccgg cgccttcatt gcggcgagcg cgcgcagcgc gcccagcacc
     3121 gaggcggcgc cggacatgtc gaacttcatc tcgtccatct cgccggcggg cttgagcgag
     3181 atgccgccgg tatcgaacgt gatgcccttg cccacgaacg cgagggattt cctgctcttc
     3241 gccccagcgc catagcgcag cacgatgagt ttcggcggct gctgtgagcc gcgcgccacc
     3301 gcgagcagcg cgcccatgcc gagcttctcc atgtctttgc gctcgagcac ttcgagctcg
     3361 agcttgaatt cgcgcgcgag cttcttcgct tcgtcggcaa gatatgccgg cgtgcagatg
     3421 ttcggcggca ggttcccaag cgtgcgcgca agcgccgagc cttccgacgt agccacggct
     3481 tcgcgcaatg catgctgcaa ttcggctttc gggctgagcg gcagcgtgac cgcggcgagc
     3541 gcacgaccgt tcgctttgtt ctgcgtcttg agctggtcga agcggtagag cgcgtcctgc
     3601 atggcgagta cggcatgacg cacgttccag ctcaccgggc gggagccgat tttcatgtcg
     3661 acgaggaaaa gtgccgcgtc cttggcgtcg agctccttca gcgcgttcgc cgcggcgcgc
     3721 accgcgtcgc gataggctgc ttccttggtg ctcttatcgc cgaggccgac gagcagcacg
     3781 cgctccgcag cgacgccggg cagcgagtgc agcagaagcg tggagccggt cttgcccgag
     3841 atatcgcgcg ccgctttctt gagcgcgcct ttcgcggcct gatcgatgcg gcgcgcggcc
     3901 gcggtgagct cgcccgcggt atgaacgccc acgaccacgc agcccgtctt cgccgactcg
     3961 ggcgagagcg tctttatgct aaattccacg ggccgaaagt atcgcatgat cttccaacgg
     4021 gcgctgctca gggagtttgc aggtctcgcg ggcgccgtat tcatgacgct gttcgccatc
     4081 gccgtgacga cgcggctcat tcgcctgctc ggccaggctg cgggcggcaa gtttccctcc
     4141 gacgcggtgt tcgcctttct cggctttttc gctctcggcg ctctgccggt gctgctgtcg
     4201 ctcacgatgt tcatctcggt gctgctgacg atgacgcgca gctggcgtga ctcggagatg
     4261 gtgatctggt tcgcttccgg acagccgctc accgcgtggc tcaggcccgt gatgctgttc
     4321 gcgctgccgc agatcgccgt gatcggcgcg ctctccctct tcatctcgcc gtgggcggcg
     4381 cagatggccg ggcagtacac gtcgaagatc gagcagcgcg acgacgtgtc gcgcgtcacg
     4441 cccggcgtgt tcggcgagac ggcgaatcgc acgcgcgtgt tcttcgtcga gtcggtctcc
     4501 ggcgactcga gcgcggtgca gaacgtcttc gtgagctcgg tgcacggcac gaagagcggc
     4561 gtgagcatga gccgcagcgg gcacaccgag accgcgccga acggcgaccg cttcatcgtg
     4621 cttgaacagg ggcgccgcta cgagggcgcg cccggcgatc ccgagtatcg ggtcaccgaa
     4681 ttcgaccgct acgccgcgcg catcgagctc aaggagggca gggaagtcac gcccacgcac
     4741 aagaacctgc cgacgttggc gctgatttcc aaccctacga accagaacct cggcgagctt
     4801 ctgtggcgca tcggcattcc gatctcggcc ttcgtgctcg tgctgctcgc catcccgatg
     4861 agcttcgtca atccgcgcgc cggccgctcg gcgaacctgc tgttcgccct gctcacctac
     4921 atcgtgtact ccaacctgct gagcgtgagc caggcgcgcg tcgctcaggg gcgcatggac
     4981 ttcggcgtgg gctggtggct tgtacacgcc ggcatggtgg tgctgctcgt catcatgttc
     5041 gcgcagcgca tgcagctcat ccgcctgaga atcggtcgat gagcggtcga tagcccgtgc
     5101 gtacgctcga acgctacctc gcgcgccaga tctacggcgc caccgctttc gtgctgctgg
     5161 gcttcctcgg gctcttcgcc ttcttcgatc ttatccgcga gctcgcggac ctcggcaagg
     5221 tcgaatacca cctgccgcag gttttcagtt tcgtgctgct ctccatgccg acccacgcct
     5281 atgagctctt tccggtggtg gtgctgatcg gcaccctcta tgtgctcgcg cttctcgcca
     5341 gcaactcgga atacacggtc atgcgtgcat cgggcctttc accacggcgc gccgggcttg
     5401 cgctcgccaa ggtcggcatc ccgttcgtcg tgctcacctt cgtaatcggc gagtgggtgg
     5461 cgccgtggtg cgaggagcag gcgcagaagg tgaagatgcg cgccatgagc tcgatgatcg
     5521 gccaggacct tacatccggc ttgtggttca aggacgaggg cgcgttcatc aatgtaaagg
     5581 aagcccggca gaccaacctc ctgaacggcg tgcgcatcta cgacttcgac gcggactatc
     5641 gcctgcagcg catcacgctc gcccagcgcg cggagtacgc gggcaagggt aactggcgcc
     5701 tcaccgaagt catgcgcacg catttcacga ccgagggtcc gcgcatcgag aagcagccgg
     5761 agctcgaatg gcgctcgcgc gtgacaccgg atatgctcga cgcgctcatc gtgcgcccgg
     5821 agcgcatgtc ggcctgggcc ctgcataagt acaccgagca cctcgccggc aaccgacagc
     5881 gcaccgagcg ctacgagatc gcgctctgga agaagctctt ctatccgctc gccgcgctcg
     5941 tcatgatggc gctcgcgctg cctttcgcct acatgcaggc gcgagccggc atggtcggcg
     6001 tcaaggtgtt cctcggcatc atgctcggca tttttttcca catgctgaac agcctcttct
     6061 cgcacatcgg cttattgcag aactggcccc cgctctccgc cgccatcgtc ccgagcctcg
     6121 ccttcctgat ggcggcgatc atgatgatgt ggtgggtgga acgccgctag gcgttcacca
     6181 gccgagtgcc ggcaaggcgg tcgtgcagaa actgccgatc ccgatcgaat agcgcccaca
     6241 ggatcgaggc cggcaaaaag gctagcgcga gcaagaaccg gagcatcgcc ttccgcggcg
     6301 taacgcccac caggcgaata tgccaggcct tcatggcgag ggtctgaccg ccgcgcagcc
     6361 agcaccagaa aaagtagccc gcgaagaatg ccgcgatgaa gacctggagc gcggtgcgca
     6421 gccagcctga agttgtgtcc tcgccgccgc ttgcgaagaa gaagagccag gcggcgaaga
     6481 acgcgacggc gaagagcagc actgcttcgt acaccatgct cgcgaggcgc cgcgcgagcc
     6541 cgggcgtcgc gccgctcagc ggcgtcggcg ctctcctggc gcggtcaggt tctcgcgctc
     6601 ctgcggcggc agctgctggt attcggccca ttgctcgcgc aggttctgcc gcttctcggg
     6661 cggggtcttg gcgatctgct tatacgtctc gcgcgcctgg cgtcgctgct cgggcgtcag
     6721 cttggcccat gcctgcatgc gccgctgcac gcgctcctgc gcctgcggct gcatcttcgg
     6781 gtagcgcttg gcgacgccga tccatttgcg gcgacgctcg ggctcgaggc tttcccaatc
     6841 cgccttgagc ggcgcgagga tctgctgctg ctcggcggtg agctccgccc aggccgggcg
     6901 cacttgcgcc ttggccttgg cggccggctt ggccttggct ggcgccgcct tgcgctggac
     6961 ttcgacgggc ggctcggcaa gcgcgccgcc gcacagcaga gcggcgacga gaagccacgc
     7021 ggcgcgcgtc attcgtccgc cgcgcgcttc ttcaaccagt tctggaagcc gcgatcgagg
     7081 taggcgtcga tcggcagatc atcggtgagg agctgggcgt cgatctcctc gacttcggcg
     7141 gcgaggcgat tctgttgcca gccgtagagc gccgcgccgc tgccgacgag gagcgcaatc
     7201 ggcaggagca cgcgcagcgc gaaaccgctc cagccgccca tgcggccgac gaggttgtcg
     7261 gcccatgcga ggcccgcgac ctcggcgcgc tggtgctcga gcgcccgcat gcgcgccgcg
     7321 gcgagccgcc tcgcaacgtc cggcctgacc ttagtcccct gagtcaggag ttgacggatc
     7381 ctgtgtccga attgcatttc gttcatgatt ttgtctcgaa cgtgataccg cgtgcgctca
     7441 acgctgcttg caaagcgtgc gcagctcggg agcaatgcgt tttcacgctg ccctccgagc
     7501 agttcatcgc ccgcgccgtt tcggccacgt ccagttcctc ccaataacgc aggatgaagg
     7561 cttcgcgttg acgcgccggc aaccgcgaaa tctcttgttc aataatttca agcacttggg
     7621 cttgttcgaa gcgctcggcc ggcgcctcct ggctattgga cccgccctca ggcagcatgg
     7681 tttccagcgg atcttgctcg tcgtcgtcct cgcgtcccgg gccgagcgac gacagaagcg
     7741 tggtccatag cgagcgcact ttctgacgcc ggtaccagtc gcgaatcgcg ttttgcatga
     7801 tgcgatgaaa aagcatcggc agctcgcccg ccgggcgcgc gccgtatttc tcggcgagtc
     7861 gcagcatcgc gtcctgcacg atgtcgagcg ccgcttcgtc gtcgcgcacg gcgaacaccg
     7921 cctgcttaaa cgcgcgcttc tcgatgcctt ccaggaactg ggaaagctcg ctgcgggatg
     7981 ccagggtggc ggccctctgt tgtttggcgc ggcatgttag caaaaggcgc tctcgcagcg
     8041 gccgcgcccg gcttgaccca acacagccaa gcctttattg tgcaaagctt ttcccccacc
     8101 agaacgaatt ttcatggatc gggccaagac caccacgctc accggcgccg aaatcgttgt
     8161 gcgctgcctc caagaagagg gcgtggaata cgtcttcggt tatcccggcg gcgcggtcct
     8221 cttcatctac gacgagctct tcaagcagga caaggtgaag cacgtgctcg tgcgccacga
     8281 gcagggtgcg gtgcatgcag ccgacggcta ctcgcgctct tccaggaaag tcggcgtcgc
     8341 gctggtgact tccggaccgg gtgtcaccaa cgccgtgacc ggcatcgcta cggcgtacat
     8401 ggattcgatt ccgctcgtgg tgatcaccgg ccaggtgccc acgcaggcga tcggcctcga
     8461 tgcgttccag gaatgcgaca ccgtcggcat cacgcgcccg atcgtgaagc acaactttct
     8521 ggtgaagaac gtcgccgacc tcgcctcgac catcaaaaag gcgttccaca tcgccgccac
     8581 gggtcgcccg gggccggtgg tggttgatat tccgaaggac gtgacgcagc acgcctgcga
     8641 gttccactac cccgacacgg tgcacatgcg ctcgtacaac ccggtgcgca agggtcacgc
     8701 cgggcagatc aagaaggcgg tgcagctgct cctggatgcg gagcgcccct tcgtgtatac
     8761 gggcggcggc gtggtgcttt ccgacgcttc ggtggagctg cgcgagctcg tgaagcagct
     8821 tggtttcccg tgcacgaata cgctgatggg gctcggcgcg tatccggcga ccgatcgcca
     8881 gttcctcggc atgctcggca tgcacggcac gtacgaagcg aacatggcaa tgcagcactg
     8941 cgacgtactg ctcgccatcg gcgcgcgctt cgacgaccgc gtgatcggca atcccgcgca
     9001 cttcgcgcag aacccgcgca agatcgtcca tatcgatatc gatccgtcct cgatctccaa
     9061 gcgcgtgaag gtcgacgtgc cgatcgtcgg caacatcccg gacgtgctcg aggacctgct
     9121 caagctcctc aagggctcga ggggcaagga catttccgcg tggtggaaac agatcgacgg
     9181 ctggcgcgcg aaggactgcc tgcgctatga ccgcaagtcg ccgatcatca agccgcagtt
     9241 cgtgctcgag aagctgtacg aggtgaccag gggcgaagcc ttcgtcacat cggacgtcgg
     9301 tcagcaccag atgtgggcgg cgcagttcta caaattcaac gagccgcggc gctggatcaa
     9361 ctccggtggc ctcggcacga tgggattcgg cctgccggcg gcgatgggcg tgcagctcgc
     9421 gaaccgcggg gcgacggtgg cgtgcgtcac cggcgagtcg tcgatccaga tgtgcctcca
     9481 ggagctctcc acctgcaagc agtaccggct gcccatcaag atcatcaatt tgaacaacaa
     9541 gtacaggggc atggtgcggc agtggcagga gttcttccac ggcaaccgct actccgagtc
     9601 ctacatggac gcgctgcccg acttcgtcaa gctcgccgag ggctacggcc actcggggct
     9661 gctggtcgac aagcccgccg acgtcgagcc ggcgctgcgc gagaccttca agcgcaagaa
     9721 cgacctggtg ttcctcgatt tcctcaccga ccaggccgag aacgtctatc cgatggtgcc
     9781 cggcggcaag ggcatcacgg agatgatcct ctccgaagac ctgtaatgcg ccacatcatc
     9841 tcgatcctcg tggaaaacga ggccggtgcg ctctcgcgca tctcgaacat gttctcggcg
     9901 cgcgcctaca acatcgagtc gctcaccgtg gcgcctaccg aggacgcctc gatgtcgcgc
     9961 atgacggtgg tgacctcggg gtcggacgac gtcatcgaga agatcaccaa gcagctcaac
    10021 aagctggtcg aggtggtgaa ggtggtcgac ctctcggagg cggagcacat cgagcgcgag
    10081 ctgatgctca tcaaggtgcg cgccggcgcc aaggagcgcg aggacatgaa gcgcatgtgc
    10141 gacatcttcc gcggccgcgt catcgacgtc tccgacaata cctacaccat cgagctcacc
    10201 ggcgagggcg ccaagctgga cgcattcatc aaggcgctcg accagggcac catcatcgag
    10261 acggtgcgga ccggggcaag cggcatcggc cgcggcaaca ggattctgag aacttgaggg
    10321 aaccaaaatg aaggtctatt acgacaagga c
//
LOCUS       DASTPD010000281         7963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_560647, whole genome shotgun sequence.
ACCESSION   DASTPD010000281 DASTPD010000000
VERSION     DASTPD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560647"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..771)
                     /locus_tag="VFR57_09350"
     CDS             complement(37..771)
                     /locus_tag="VFR57_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0259608.1"
                     /translation="MTETAYLSLLGDRVRAWRSEHGMTRKGLAAASGVSERYLAQLEA
                     GEGNISVLLLRKIARAMGVPVESLVREDAPREKSIALLGLRGAGKSTLGEKLAEALKL
                     PFIELDREVEKEAGAELGEVFAMYGQDAYRRFERRALERVLAQNERAVIATGGSLVTD
                     AGTYKTLRERCLCVWLKASPEEHMARVIAQGDMRPFKGRSAALDEIRQLLADRDRLYS
                     RADVTLDTSGKTLRASLSQLLSLVKT"
     gene            822..1286
                     /locus_tag="VFR57_09355"
     CDS             822..1286
                     /locus_tag="VFR57_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU0259609.1"
                     /translation="MKVTILVGTMTGTAQLCAQEMELALDDGETEVATLMMDGLDSSV
                     FGRDGVFLICTSTYGQGDVPDNARALYEDLKSVRPDLSRVRYGVFGLGDRTYAETFNF
                     GGKRFDEILTDLGAKRIGERHMHDASSGILPEETALQWCQDWLAKAKETDTV"
     gene            1327..3339
                     /locus_tag="VFR57_09360"
     CDS             1327..3339
                     /locus_tag="VFR57_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0259610.1"
                     /translation="MRALKWVGGVVLALFGAIALFFAFGLSTLRGPIADAVTKSTGRE
                     LVIGGGIRPVWSWVHPRIRIEGVTFANADWGKADYLLNANAVEASISVLPLLAGRVVL
                     PEVHLEGAELALERDAEGRRNWLLKDEEDKKEESRFFIKLLTVDDGRVIWDDATTDTR
                     VEADLSTDDTGVAFEAQGKWNGTKMKGAGHAGHVLSIRDDSTPFPLKGEITIGQTSAK
                     LDGTVTGIVGFKGIDLQFERLSGQSMDDLYNIIGLALPATSPYTVSGRLYRTDGAWMF
                     ENFVGKVGESDLAGTIRVQTAEGKRPLMQGEINAKVLNFADLGVLVGTGEPRKSGVLP
                     DMPFAPERWNSVDADVKINAGTIKRPKQLPIEDFSMRIRMDERVMTLQPLAFGIAGGR
                     LEGTVRLDGNYSPLRGDVKMRVQNLQLAKLFPTVKQAQGSIGDLNGLIELAGTGDSVG
                     KLLGTSNGKIGVYMDEGKISRFLMELVALDLWDAARVKLRGDKEIDIRCAIADFGVKA
                     GMMEANAIVFDTGVVNVQGRGSINLKTEEMDITLKPQPKDGSIASLNSPLYIRGTFGT
                     PNIGPDMGRLAAKGAGAIVMGILNPLLAVLPLVEEGKGKDSPCRQLIAEATKSAKTSA
                     AAKAKEAKVKEAKAKEAKPKEAKAASSDQSPASGATAPSPPSTPER"
     gene            complement(3263..4213)
                     /locus_tag="VFR57_09365"
     CDS             complement(3263..4213)
                     /locus_tag="VFR57_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259611.1"
                     /translation="MRWLLTALALVVSVARAQEPPFPQRGPVEITVLFPAGSSADVTA
                     RLLADGMSRNLGQRVLVVNRPGAGGAIGYKHVASQKSDGYSLVWNSNSISTTFHSGQL
                     NFDYRAFDAVARVLSESVVLAVRADAPWRTLAELIAEAKAKPKTISVGHSGVGSHTHI
                     SAAALFGATGVEVTEVPFAAAQVVPSLVGGHVQAVVQFPAALAAPLSQKQIRLLVALT
                     QTRDPAWPDVPTARELGYDVALDAWRGIAVPRGTPPQVISALENAIRTTTSSPEFVSA
                     AEKLGVRPAFLAAQEFSVLVSKEDLELSRLMQVIGLKKQP"
     gene            complement(4210..5286)
                     /locus_tag="VFR57_09370"
     CDS             complement(4210..5286)
                     /locus_tag="VFR57_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0259612.1"
                     /translation="MHRRGRRCRRQFRVRRARRQGSGRRCRQVRVPMRVACLGMGWWS
                     DVLADAMQRSGKLTIVSCYSRSTEKREAFAKKYGCRAARSYEEILDDNSIEGIINTTP
                     NSMHRTTTIAAAKAGKHTFLDKPIANTIDDARALTKACRQASVVLALGYQRRRESHFR
                     WIKENLGQFGRLVNAEANISRDRLGKIDLSSWRYTAEGMPGGVMLQIGIHYTDVLEYL
                     MGPIRAVSGRFVRLVLPGDNPDVASLVLEHENGALSTLNASYASASEHYLMNIYGKEA
                     TAYYDLHGGLRWLRRGSSKTDTITVRKNDPIVEELEEFARAARGDGEPEMNGEKGTAS
                     LAVLLAGIKSAREGRRVELKEVLG"
     gene            complement(5199..5630)
                     /locus_tag="VFR57_09375"
     CDS             complement(5199..5630)
                     /locus_tag="VFR57_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0259613.1"
                     /translation="MYIADAKRLTAAGARKIVDSAVEKAKRENIAITIAVVDAGGHLM
                     LLERMDGGKFHSVHSATTKAVCAASNKRPTGSTGAVGQSLDMLHALGLALAAGPERWT
                     AMEGGCPIMAGGECIGGVGVAGGNFEFDERVAREAVAAVAK"
     gene            5647..6114
                     /locus_tag="VFR57_09380"
     CDS             5647..6114
                     /locus_tag="VFR57_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259614.1"
                     /translation="MPLSHIEHLLVAADDIDATRDWYARVLGMTSGPHPDFGFPVHWM
                     YLNGVDVVHIGPSAKTAGAIQKQYLGRTSQGAGQGTGAIDHIAFRASGLREMMVHLKD
                     EGIDFRQRRANGQALFQLFFHDPNGIKIELNFDAAEAEGIAPELMASDLIRAK"
     gene            6111..7718
                     /locus_tag="VFR57_09385"
     CDS             6111..7718
                     /locus_tag="VFR57_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEU0259615.1"
                     /translation="MSAVVVERQLQLALDNMPGALAYTDEGLNLVFCNDRFREMYKVP
                     PKLLQPGAHYPELLRYLADHGYYGPGNVEEVVARRIESLRNPSGRAFEDQAPDGRWYR
                     VLRRRIAGRGTITVMTDITEQKLAEAQLAEREAQLHVALDNMPGALAYTDENLKLVFC
                     NDRFKEMYIVPPELLQSGRPYPDLLRYLAENGYYGPGDVDEQVARRVESLRNPSGHSL
                     EDHAPDGRWYRILRRRVAGGGTVTVMTDITVEKQAERDLLEATRRTEEANKVITEKSR
                     VLEALNAEIRDKNVQLQEQAAQIAEWNARLEMRVTEQVAQIGQMSKLTRFLSPKVSDL
                     IMAGDADDPLKTRRRDITVVFVDLRGFTGFTETAEPEEVMSVLREYHAELGKAIMAHD
                     GTIEHFAGDGVMILFNDPMPVPDHELQAIRMAITMRDSVGLLVRAWKKRGHALGFGVG
                     IAGGYATIGTIGFEGRLDYGAIGAVTNLAARLCGEAQHDQILISPRIFSKVEDRIEAQ
                     SVGELQLKGFQRAVLAHNVIRLRPQPT"
     gene            complement(7785..7955)
                     /locus_tag="VFR57_09390"
     CDS             complement(7785..7955)
                     /locus_tag="VFR57_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259616.1"
                     /translation="MLGTVDAAMEVAGGASFFRANGLERLFRDAQGARFHPLQEGLQK
                     ALTARLALGLEI"
BASE COUNT         1459 a         2553 c         2582 g         1369 t
ORIGIN      
        1 agtggttgta gcgatcgggt tgggtctcga aagagatcat gtctttacca gagagaggag
       61 ctgggagagg ctcgcgcgca gcgtcttgcc ggaagtatcg agcgtgacat cggcgcggct
      121 gtagaggcgg tcgcgatcgg caaggagctg gcggatttcg tccagcgccg ccgagcgtcc
      181 tttgaatgga cgcatgtcgc cttgagcgat aacgcgcgcc atgtgctctt ccggcgaggc
      241 cttcaaccac acgcacaggc agcgctcgcg cagcgtcttg taggtccccg cgtccgtaac
      301 aaggcttccg ccggtagcga tcaccgcccg ctcattctgc gccagcacac gctctagcgc
      361 gcggcgttcg aatcggcgat aggcatcctg gccgtacatg gcgaacactt cgcccagctc
      421 cgcgccggct tccttctcga cttcgcggtc gagctcgatg aacggcagtt tgagcgcttc
      481 ggcgagcttt tcgccgagcg tcgacttccc ggcaccccgt aagccgagga gggcgatgga
      541 cttttcgcga ggcgcgtcct cgcgcaccag gctttcaacc ggaaccccca tcgcgcgggc
      601 gatcttgcgc agcaggagca ccgagatgtt gccttcaccg gcctcgagtt gtgcgaggta
      661 gcgctcggaa actccgctcg ccgcggcgag ccccttgcgc gtcatgccgt gctcgctgcg
      721 ccaggcgcgc acacggtctc cgagcagcga gaggtaggcg gtttcagtca tgcagtatag
      781 tgcttgactg gcattctatg ggtgcaatat attgactgtc aatgaaagtg accatcctgg
      841 tcggcacgat gaccggcacg gcgcagctct gcgcgcaaga gatggagctc gcgctcgacg
      901 acggcgagac cgaggtcgcg acgctgatga tggatggcct cgacagcagc gtcttcggcc
      961 gcgacggcgt tttcctcatc tgcacttcga cttacggcca gggcgacgtc cccgacaacg
     1021 cgcgggcgct ttatgaggac ctgaagagcg tgcggccgga cttgtcgcgc gttcgctacg
     1081 gcgtctttgg actgggcgac cgcacctacg ccgaaacctt caacttcggc ggcaagcgct
     1141 tcgacgagat ccttaccgac ctcggcgcaa agcgcatcgg cgaacggcac atgcacgacg
     1201 cttcgagcgg catccttccc gaggaaaccg cgctccagtg gtgccaggac tggctcgcga
     1261 aagcgaagga aaccgacacg gtgtaggact tcacctgacc ggcacgcact ttgctggatg
     1321 gttcatatgc gtgctctgaa gtgggtcggc ggcgtggtac tcgccctctt tggcgcgatc
     1381 gcgctctttt tcgcgttcgg cctgagcacg ctacgcggtc ccatcgccga cgcggtgacc
     1441 aagtccaccg gtcgcgagct cgttatcgga ggcggcatca ggccggtctg gagctgggtg
     1501 catccccgca tccggatcga gggtgtcact tttgccaacg ccgattgggg aaaagcggac
     1561 tacctcctta acgccaacgc cgtcgaagcg agtatcagcg tcttgccgct cttggcgggc
     1621 cgcgtcgtcc tgcctgaagt ccatctcgaa ggcgcagagc ttgcgctcga gcgcgacgcg
     1681 gagggccggc gcaactggct tttgaaggac gaggaggaca agaaggaaga atcgcgcttc
     1741 ttcatcaagc tgctgacggt cgacgacggc cgggtgatct gggacgacgc taccacggac
     1801 acgcgcgtgg aggcggacct ttccaccgac gacaccgggg tcgccttcga ggcgcaaggc
     1861 aagtggaacg gtacgaagat gaagggcgcg ggtcatgccg gccacgtgct ttcgatccgc
     1921 gacgactcca cgccgtttcc gctcaagggc gagatcacga tcgggcagac ctcggcgaag
     1981 ctcgacggca cggttaccgg cattgtcggc ttcaagggca tcgatctgca attcgagcgc
     2041 ctgtccgggc agtcgatgga cgacctttac aacatcatcg gccttgccct gccggcgacg
     2101 agcccgtata cggtgagcgg gcgcctttat cgcaccgacg gggcctggat gttcgagaac
     2161 ttcgtcggca aggtcggtga gagcgacctt gcgggcacga tccgcgtgca gaccgccgag
     2221 ggcaagcggc cgctcatgca gggcgaaatc aacgccaagg tgctgaattt cgccgatctt
     2281 ggcgtgctgg tcggcacagg cgagccgcgg aaatccggcg tactgcccga catgccgttc
     2341 gcgccggagc gctggaacag cgtcgacgcc gacgtgaaga tcaatgccgg caccatcaag
     2401 cgccccaagc agctaccgat tgaggacttc agcatgcgca tccgcatgga tgagcgggtg
     2461 atgacgcttc agccgctcgc cttcggcatc gcgggcggac ggctcgaagg cacggtgcgg
     2521 ctcgacggca attactcgcc gttgcgcggc gacgtaaaaa tgcgcgtgca aaacttgcag
     2581 ctcgcgaagc tctttccgac cgtgaagcag gcgcaggggt cgatcggcga cctgaacggg
     2641 ctgatcgagc tcgctggcac cggagattcg gtgggcaagc tcctcggcac ctcgaacggc
     2701 aagatcggcg tctacatgga tgagggcaag atcagccggt tcctcatgga gctcgtcgcg
     2761 ctggatctct gggacgcggc gcgcgtcaag ctgcgcggcg acaaggagat cgacatccgc
     2821 tgcgcgatcg cggacttcgg cgtgaaggcc ggcatgatgg aggctaacgc gatcgtgttc
     2881 gataccgggg tggtgaacgt gcagggccgc ggcagcatca acctcaagac cgaggaaatg
     2941 gacatcacgc tgaagcccca gcccaaggat ggcagcatcg cctcgctgaa tagtccgctc
     3001 tacatccgtg gcacgttcgg cacgccgaat atcggccccg acatgggtcg gcttgccgca
     3061 aagggcgcgg gcgcaatcgt catgggaatc ctgaatccgc tgctcgcggt gctcccgctc
     3121 gtggaagagg gcaaaggcaa agactcgccg tgccgccagc tcatcgcgga ggcgacgaag
     3181 tcggcgaaga cgtcggcggc ggcgaaggcg aaggaagcca aggtaaaaga agccaaggcg
     3241 aaagaagcga agccgaaaga agctaaggct gcttcttcag accaatcacc tgcatcaggc
     3301 gcgacagctc caagtcctcc ttcgacacca gaacgctgaa ttcctgcgct gccaggaacg
     3361 cggggcgcac accgagcttt tccgcggccg agacgaattc cggtgagctc gtcgtcgtac
     3421 gaattgcgtt ctccagcgcg gagatcacct gcggcggcgt gccgcgcggc acggcgatgc
     3481 cgcgccaggc atcgagcgcg acgtcgtagc caagctcgcg cgccgtcgga acgtcgggcc
     3541 acgccggatc gcgcgtctgc gtgagcgcga cgagcaaacg gatctgtttc tggctgagcg
     3601 gggcggcgag cgccgccgga aactgcacca ccgcctggac gtgcccgccg accaggctcg
     3661 gcaccacctg cgctgcggcg aagggcacct cggtcacctc cacaccggta gccccgaaga
     3721 gcgccgcagc cgagatatgc gtatggctgc ctacgccgga atgcccgacg ctaatcgtct
     3781 tcggcttcgc cttcgcctct gcgatgagct cggccagcgt gcgccatggc gcgtccgctc
     3841 gcacagcgag gaccaccgac tcggacagaa cgcgcgccac ggcgtcaaac gctcggtagt
     3901 cgaagttaag ctggccggaa tggaatgtgg tcgagatcga gttcgagttc cacacgagcg
     3961 aatagccgtc cgacttttgc gatgcgacgt gcttgtagcc gatcgcgccg cccgcgcccg
     4021 gccggttcac caccaacaca cgctgaccga ggttgcgcga catgccgtcg gcgagaaggc
     4081 gggccgtgac atcggccgac gaaccggccg gaaacagcac ggtgatctca acgggcccgc
     4141 gctggggaaa tggcggctct tgtgctcttg ccaccgaaac gaccaaagcc aatgcggtca
     4201 aaagccatct catcccaaca cctcctttag ttcgacgcgt cgaccctcgc gcgccgactt
     4261 gattccggcg agcagcacgg cgagcgatgc ggtgcctttc tcgccgttca tctcgggctc
     4321 gccgtcgccg cgggcagccc gcgcgaactc ctcgagctcc tccacgatcg gatcgttctt
     4381 gcggacggtg atagtgtcgg tcttgctcga cccgcgtctt agccagcgca ggccgccgtg
     4441 caagtcgtaa tacgcggtcg cttccttgcc gtagatgttc atcaggtagt gctcggaagc
     4501 cgatgcgtag ctcgcgttga gggtcgagag cgcgccgttc tcgtgttcga ggacgaggct
     4561 cgccacatcc gggttgtccc cggggaggac gaggcgcacg aaacgcccgc tgaccgccct
     4621 aattggtccc atcaggtact cgagcacatc ggtgtaatgg atgccgatct gcagcatcac
     4681 gcctcccggc atgccttccg ccgtgtagcg ccatgagctg agatcgatct ttcccagccg
     4741 gtcgcggctg atgttcgcct ccgcgttcac cagtctgcca aactgaccga ggttctcctt
     4801 gatccagcgg aagtggcttt cgcgcctgcg ctggtagccg agcgcgagca ccacgcttgc
     4861 ctggcgacac gccttggtga gcgcgcgcgc gtcatcgatc gtgttggcaa tcggtttgtc
     4921 cagaaacgtg tgcttgcctg ccttggccgc cgcgatcgtg gtcgtgcgat gcatcgagtt
     4981 cggcgtcgtg ttgataatgc cctcgatcga attgtcatcg aggatctctt cgtacgagcg
     5041 cgcggcgcgg caaccgtact tcttcgcgaa cgcttcgcgt ttttccgtcg agcgcgaata
     5101 gcacgaaacg atggtcagct ttcccgagcg ctgcatggca tcggcgagca cgtccgacca
     5161 ccagcccatg cccaaacatg cgacccgcat cggcaccctc acttggcgac agcggcgacc
     5221 gcttccctgg cgacgcgctc gtcgaactcg aaattgccgc cggcaacgcc gaccccgccg
     5281 atgcattcgc cgcccgccat gatcgggcag ccgccttcca tcgcggtcca gcgctcgggt
     5341 cccgcggcga gcgcgaggcc gagcgcgtgc agcatatcga gcgactgtcc gaccgcgccg
     5401 gtggagccgg tcggacgctt gttcgaagcc gcgcataccg ccttggtggt agcggagtgc
     5461 accgaatgga acttgccccc gtccatccgc tcgaggagca tgagatggcc tccggcgtcg
     5521 accacagcga tcgtgatggc gatgttctcg cgcttcgctt tctcgacggc gctgtcgaca
     5581 atcttgcgcg cacccgcggc ggtgaggcgt ttagcgtcgg caatgtacat ggttgtatat
     5641 tagtgcatgc ctctttcgca catcgagcac ttgctcgtcg ccgccgacga catcgacgcg
     5701 acgcgcgact ggtatgcgcg cgtattgggc atgaccagcg gtccgcatcc ggacttcggc
     5761 ttcccggtgc actggatgta cctgaacggc gtcgacgtcg tgcacatcgg cccgagcgcg
     5821 aagacggcgg gcgcgatcca gaagcagtac ctcggccgca cctcgcaagg cgccgggcag
     5881 ggaacgggcg ctatcgatca catcgcgttc cgcgcgagcg gcctgcgcga gatgatggtt
     5941 cacctcaagg atgaaggcat cgacttccgt cagcgccgcg ccaacgggca ggcgttgttc
     6001 caacttttct tccacgatcc caacggcatc aagatcgagc tcaacttcga cgccgcggaa
     6061 gccgagggca tcgctcccga attgatggcc tccgatctca tccgcgcgaa atgagcgccg
     6121 ttgttgtaga gcggcagctc cagcttgccc tcgacaacat gccgggcgcg cttgcctata
     6181 cggacgaggg cctgaacctc gtcttctgta acgaccgttt cagggagatg tataaggttc
     6241 cgccgaagct gctgcagccg ggcgcgcact atcccgagtt gctgcgttac ctcgccgatc
     6301 acggttacta cgggccgggc aacgttgagg aggtcgtggc gcggcgcatc gagagtctgc
     6361 gcaatccctc gggacgcgcc ttcgaggatc aggcgccgga tgggcgctgg taccgggtac
     6421 tgcgccgccg tatcgcggga cgaggcacga tcaccgtaat gaccgacatc accgagcaga
     6481 agctcgccga ggcgcagctt gctgagcgag aagcacagct gcatgtcgcc ctcgacaaca
     6541 tgccgggcgc actcgcttac accgacgaga acctgaagct cgtcttctgc aatgaccgtt
     6601 tcaaggagat gtacatcgtg ccgcccgagc tgctgcagtc gggccgcccg tatccggacc
     6661 tgctgcgtta tcttgcggaa aacggttact acggcccggg cgacgtcgac gaacaggtcg
     6721 cgcggcgggt cgaaagtttg cgcaacccgt ccgggcacag cttggaggac cacgcgccgg
     6781 atggtcgctg gtaccgcatc ctccgccgcc gcgtggcggg cggcgggacg gtcaccgtga
     6841 tgaccgatat cacggtggag aagcaggcgg agcgcgacct cctcgaggcg acgcgccgca
     6901 ccgaggaagc caacaaggtg atcaccgaga agagccgcgt gctcgaagca ctcaacgcgg
     6961 agatccgtga caagaatgtc caactgcagg agcaggcggc gcagatcgcg gaatggaatg
     7021 cgaggctgga gatgcgggtg accgagcagg tagcccagat cggccagatg tccaagctga
     7081 cccggttcct gtcaccgaag gtcagcgacc tcatcatggc tggcgacgcc gatgatccgc
     7141 tgaagacgcg taggcgggac attaccgtgg tgttcgtcga cctgcgcggc ttcaccggtt
     7201 tcacggaaac ggccgagcct gaggaagtga tgagcgtgct gcgcgaatac cacgcggagc
     7261 tgggcaaagc catcatggcg catgacggca ccatcgagca cttcgccggt gatggggtca
     7321 tgattctgtt caacgatccg atgccggtgc cggaccatga gctccaggcg atccgcatgg
     7381 cgatcacgat gcgcgactcg gtagggctgc tcgtgcgcgc gtggaaaaag cgcggccacg
     7441 cgcttggctt cggggtcggc atcgccggcg gctacgcgac catcggcacc atcggcttcg
     7501 agggccggct cgactacggc gccatcggcg cagtgacgaa cctggccgcg cgcctctgcg
     7561 gcgaagcgca gcacgaccaa atcctcatct cgccgcgcat attctccaag gtcgaagacc
     7621 gaatcgaagc gcagtccgtg ggggaactac agctgaaggg tttccagcgt gcggtgcttg
     7681 cccacaacgt gatccgcctg cgcccgcagc cgacctgagg ctcgctttcc gaattatccg
     7741 gtccgttcaa ccgctgcgca gcgttcgctg catcctgata agggtcagat ctccaagccg
     7801 agggccaggc gcgccgtaag agccttctgc aggccttcct gcagcgggtg gaagcgcgcg
     7861 ccctgcgcgt cgcggaacag gcgctccagg ccgttggcgc ggaagaaaga cgcgcctccg
     7921 gccacctcca tcgccgcgtc gaccgtgccg agcacgccgc gcg
//
LOCUS       DASTPD010000282         5616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1377950, whole genome shotgun sequence.
ACCESSION   DASTPD010000282 DASTPD010000000
VERSION     DASTPD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377950"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFR57_09395"
     CDS             complement(<1..229)
                     /locus_tag="VFR57_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259617.1"
                     /translation="MRMPKLAPISAMLLAGAAQAADPATIDWSGVPATKLTLFYPGQS
                     TFQWLRSSGHQGAAVVGSGTPCVACHKGQEAK"
     gene            343..783
                     /locus_tag="VFR57_09400"
     CDS             343..783
                     /locus_tag="VFR57_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259618.1"
                     /translation="MKMRTLFAAATLAAVFALPALAQQMKTVGGIVVNIGVVNAITAE
                     HADAQHGVHKGGHGAGMEHIVVALAEEKGGARIADADVTIAVRNPKGALQKKAALPMT
                     TAGYPDYSEVFDFGSSGKYTVTVLVKRRGADRPAEATFVINRHL"
     gene            849..1397
                     /locus_tag="VFR57_09405"
     CDS             849..1397
                     /locus_tag="VFR57_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0259619.1"
                     /translation="MTNAPALDGPIAAWHLEHECFRRLLHRLQREVDRFHGAERPNYE
                     LMYDIVSYLRDYGDLFHHPREDVAFARLVQHCSEMKLPLARLEQEHRVIAQAGEALQR
                     HIEAILNGSMVLRSDVEVAAAMYLAYYSGHIAKEEDDVLTRAVLHLTPEDWKAVKDCA
                     PGGSDALVRAAMPKRYQDLIAG"
     gene            1418..1864
                     /locus_tag="VFR57_09410"
     CDS             1418..1864
                     /locus_tag="VFR57_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0259620.1"
                     /translation="MYKHILIPTDGSEVAEKGVVAGVEYARGVGATVTFFTAVPEYQP
                     PSEGEVLARAKVVSFAEHEQNAKHLAERILATAVARARAAGLVFSTDHECKNQPWEAI
                     VDAAKRHGCDAIIMGSHGRKGLSRLFHGSQAIDVLTHSELPTLVVR"
     gene            1867..2346
                     /locus_tag="VFR57_09415"
     CDS             1867..2346
                     /locus_tag="VFR57_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259621.1"
                     /translation="MNEWTAQLYAHAIAIETEAAQRYSDLGDAMAEQGQHSVAVLFFM
                     LSSFEARHLRELTRSTAGRVLPKVKADYSWREGEAPETVDFDACVGQITPQRALLMAL
                     DAEKRAKAFFEHAARVTKDPATRALAREMAEEEAEHMILVQRALERLISHIHPRPGS"
     gene            2380..4875
                     /gene="napA"
                     /locus_tag="VFR57_09420"
     CDS             2380..4875
                     /gene="napA"
                     /locus_tag="VFR57_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HEU0259622.1"
                     /translation="MTEKITRREFMIRSVAATAATGVTLPAAAQDIVTDAKATQLKWS
                     KAPCRFCGVGCGVNVAVKDGQVVATHGDPQSEVNRGVNCVKGYFLSKIMYGEDRLTRP
                     LLRMKGGKYDKNGEFTPVSWDQAFDVMADKWKAALKAKGPSAIGMFMSGQSTIWEGYA
                     AVKLMKAGFRSNNIDPNARHCMASAAVGFIRTFGIDEPMGCYDDIEHADAFVLWGSNM
                     AEMHPILWQRVADRRLGHPHVRVAVLSTFEHRSYDLADLALTFQPQSDLAILNYIANY
                     IIQNKAVNRDFVSKHVAFRLGNPDIGYGLRPEHELQKKAANAADPGGWKPSTLDEFAK
                     FVAKYDVKFASKISGVAPERLQRLAQLYADPKTKVTSFWTMGVNQHTRGVWVNHMIYN
                     LHLLTGKIAQPGNSPFSLTGQPSACGTAREVGVFSHRLPADMVVMNPKHRAIAEEIWK
                     LPSGTIPEKPGFHAVLQSRMLKDGQLNCYWTQTTNNVQAGPNSQQEIWPGWRKPENFV
                     VVSDVYPTMSTSGADLILPSAMWVEKEGAFGNAERRTQFWHQLVNAPGEARSDLWQIM
                     EFSKRFKVEEVWPTELLAKAPQYKGKSLFDVLFANGEVNKFPNSQLDPQYENHEAKHF
                     GFYAHKGLFEEYRRFGNGKAHDLADFDTYHKVRGLRWPVVDGKETKWRFSEGNDPYVK
                     KGTGFQFYGNPDNKAYIYAVPYEPPAESPDKEYPYWFVTGRVLEHWHTGTMTRRVPEL
                     HLAFPSAVAFLHPEDAKALGVRRGDEIEIASRRGAMRSRVETRGRVKVPRGQVYVPFF
                     DASQLVNKVTLDATDPISLQTDFKKCACRISKV"
     gene            4872..5312
                     /locus_tag="VFR57_09425"
     CDS             4872..5312
                     /locus_tag="VFR57_09425"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEU0259623.1"
                     /translation="MRAVCLLALFLLLGSAFAQHDPLRPAVPFEQEPKTAPLVNAENN
                     DRKRVRSYAMQPPTIPHKTDNYQVDKNANKCMTCHARTRASETQATPISISHYQDRDG
                     NYLADVSPRRYFCDQCHVVQMQTEPTVKNRFQDVDTLIKKQTKK"
     gene            5312..>5616
                     /locus_tag="VFR57_09430"
     CDS             5312..>5616
                     /locus_tag="VFR57_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEU0259624.1"
                     /translation="MRELLRRYWRTLTTPSKFYSLGFLTIGGFIGGVLFWGGFNTAME
                     FTNTEQFCVSCHEMRDNVYQEIQPTIHFTNRSGVRAICSDCHVPHNWTFKMARKMQAS
                     "
BASE COUNT         1127 a         1830 c         1761 g          898 t
ORIGIN      
        1 gcttggcttc ctgccctttg tgacaggcaa cgcaaggcgt gccagagccg acgactgcgg
       61 caccctgatg tccggagctg cgcagccact gaaacgtcga ctgcccggga tagaacagtg
      121 tgagtttcgt ggcgggcacg ccgctccagt cgatggtggc gggatccgcg gcttgcgcgg
      181 ccccggcaag cagcatggcg gatatcggtg cgagcttcgg cattctcatg cgctcctcct
      241 gaggtatttg cggatggtgg ccgcacggcg ggcgtcgcgt ttgatctacg tcaatccggc
      301 gcgaggtgcc catgctccac tggcagcaga aggaggcgga tcatgaagat gcggacactg
      361 tttgcagcag cgacgctcgc cgcggttttc gccttgccgg ccttggcgca gcagatgaag
      421 acggttggcg gcatcgtggt caacatcggc gttgtcaatg ccatcacagc cgagcacgcg
      481 gatgcgcagc acggcgtgca caaaggcggc catggcgccg gcatggagca catcgtggtc
      541 gcgcttgccg aagagaaggg cggggcgcgc atcgccgacg ccgacgtcac gatcgcggtg
      601 agaaatccga aaggcgcgct gcagaagaaa gctgcgctgc caatgacgac cgcaggctac
      661 ccggactaca gcgaggtctt cgacttcggc agctccggca agtacaccgt cacggtcctg
      721 gtcaagcgca gaggcgcgga ccggccggcg gaagccacgt ttgtcatcaa ccggcatctg
      781 taggcgtgcc gcggctggaa tttgaccggg atcaaaactc tcaccggccc ggcgggcagc
      841 atggcgacat gacaaacgcg cctgccctgg acggcccgat cgcagcctgg cacctggagc
      901 acgaatgctt ccggcggctt ctccaccgcc tgcagcgcga ggtcgacagg ttccatggcg
      961 ccgagcgccc gaactacgag ctcatgtacg acatcgtgtc ctacctgcgc gactacggcg
     1021 atctgttcca tcatccgcgc gaagacgtcg cgttcgcgcg gctcgtacag cactgctccg
     1081 agatgaaact gccgctcgcg cgcctggagc aggaacatcg ggtcatcgcg caggccggcg
     1141 aagcgctcca gcggcacatc gaagccatcc tcaacggctc tatggtgctg cgctccgacg
     1201 tcgaggtcgc ggcggccatg tacctcgcgt actacagcgg ccacatcgcc aaggaagaag
     1261 acgacgtcct aacgcgggcc gtgctgcacc tcacgccgga agactggaag gccgtcaagg
     1321 attgcgcgcc tggcggctcg gatgcgctag ttcgcgccgc catgccgaag cgctatcagg
     1381 atctcatagc aggctgacca agagaaaggg ctcattcatg tacaagcaca tcctcattcc
     1441 caccgacggc tcggaggtcg ccgagaaagg cgttgtcgcc ggcgtcgaat atgcccgcgg
     1501 ggtcggggcg accgtcacgt tctttaccgc agtgcccgaa taccagccgc cgagcgaagg
     1561 cgaggtgctc gcgcgcgcga aggtggtttc tttcgctgag cacgagcaga acgccaagca
     1621 tctggccgag cgcatccttg caaccgccgt agcgcgcgcg cgggcggcgg gccttgtgtt
     1681 cagcaccgac cacgagtgca aaaaccagcc ttgggaggcg atcgtcgacg cggcgaagcg
     1741 ccacggctgc gacgccatca tcatgggctc gcacgggcgc aaggggttgt cgcggctctt
     1801 ccatggcagc caggccatcg acgtcctcac gcattcggag ttgccaacgc tcgttgtgcg
     1861 ctagacgtga acgaatggac ggcgcagctc tacgcgcatg cgatcgcgat cgagacggag
     1921 gccgcccaac ggtatagcga cctcggcgat gcgatggcgg agcagggaca gcactcggtc
     1981 gccgtattgt ttttcatgct gtcctcattc gaggcccggc atctcaggga actgacgcgg
     2041 agcacggccg ggcgcgttct tcccaaagtc aaggccgact acagctggcg cgaaggcgag
     2101 gcgccggaaa ccgtagactt cgacgcttgc gtggggcaga tcaccccaca gcgcgcactt
     2161 ctgatggctc tcgacgccga gaagcgggcc aaggccttct tcgagcacgc cgcacgcgtc
     2221 accaaagacc ccgccacgcg agcgctcgcg cgcgagatgg ccgaggaaga ggccgagcac
     2281 atgattctcg tccagcgcgc gctcgagcgc ctcatttcgc atattcaccc ccgcccgggc
     2341 tcatgagttg ctagcgtctc ggtcggagcc ggaggacgca tgaccgagaa aatcacgcga
     2401 cgcgaattca tgattcgctc cgttgcggcg actgctgcga ctggagtcac gctgccggct
     2461 gcggcgcagg atatcgtgac cgatgcgaaa gccacgcagc tcaaatggtc caaagcgccg
     2521 tgccgcttct gcggggtcgg ctgcggggtg aacgtcgccg tcaaggacgg tcaggtcgtc
     2581 gcgacacacg gcgatcccca gtcggaagtg aaccgcggcg tgaactgcgt gaagggctac
     2641 ttcctctcga agatcatgta cggcgaggac cgcttgacgc ggccgcttct gagaatgaaa
     2701 ggcgggaaat acgacaagaa cggcgagttc acgccggtga gctgggacca ggcgttcgac
     2761 gtgatggccg acaagtggaa ggccgcgctg aaagcgaaag ggccttccgc gatcggcatg
     2821 ttcatgtccg gccagtcgac gatctgggaa ggctacgcgg cggtaaagct catgaaggcg
     2881 ggcttccgct cgaacaacat cgacccgaat gcgcgtcact gcatggcgag cgcggcggtg
     2941 ggattcatcc gcaccttcgg catcgacgag ccgatgggct gctacgacga catcgagcac
     3001 gccgacgcgt tcgtgttgtg gggatcgaac atggccgaaa tgcatccgat cctgtggcag
     3061 cgcgtcgccg accggcgcct cggccatccg cacgtgcggg tcgccgtgct ctcgaccttc
     3121 gagcacagga gctacgacct cgccgatctc gcgctcacct tccagccgca gtcggacctc
     3181 gcgatcctga actacatcgc caactacatc atccagaaca aggcagtgaa ccgcgacttc
     3241 gtctcaaagc atgtcgcctt ccgcctcggc aaccccgaca tcggctatgg tctgcggccc
     3301 gagcacgagc tgcagaagaa ggcggcgaac gcggccgacc cgggcggctg gaaaccctcc
     3361 acgctggatg agttcgccaa gttcgtcgcg aagtacgacg tcaagttcgc gtcgaagatt
     3421 tccggcgtag cccccgagcg cctgcagcgg ctcgcgcagc tctatgctga cccgaagacc
     3481 aaggtgacgt cgttctggac catgggggtc aaccagcaca cgcgcggcgt gtgggtgaac
     3541 cacatgatct ataacctgca cctccttacc ggaaagatcg cgcaacccgg caacagtccg
     3601 ttctcgctca ccggccagcc ctcggcgtgc ggcaccgcgc gcgaggtcgg cgtgttctcg
     3661 caccggctac cggccgacat ggtcgtcatg aacccgaagc accgcgccat agccgaggag
     3721 atctggaagc ttccgtcggg cacgattccg gagaaacccg gattccatgc ggtcctccag
     3781 agccgcatgc tcaaggacgg ccagctcaac tgctactgga cgcagaccac gaacaacgtg
     3841 caggcggggc cgaactcgca gcaggaaatc tggcccggct ggcgcaagcc cgagaacttc
     3901 gtcgtcgtct ccgacgttta tccgacgatg agcacgtcgg gcgccgatct catcttgccc
     3961 tccgcgatgt gggtggaaaa ggaaggcgcg ttcggcaacg cggagcggcg cacgcagttc
     4021 tggcaccagc tcgtcaacgc gccgggcgag gcgcgctcgg atctttggca gatcatggag
     4081 ttttccaagc gcttcaaggt ggaggaggtg tggcccaccg agctcctcgc caaggcgccg
     4141 caatacaagg gcaagtcgct cttcgacgtg ttattcgcca acggcgaagt caacaagttc
     4201 cccaactcgc agctcgatcc gcagtacgag aaccacgaag cgaagcattt cggcttctac
     4261 gcgcataagg gccttttcga ggaataccgg cgcttcggca acggcaaggc gcacgacctc
     4321 gcggacttcg atacctacca caaggtccgc gggctgcgct ggcccgtcgt cgacggaaaa
     4381 gagaccaagt ggcgattcag cgaaggtaac gacccatacg tgaagaaggg aactggattc
     4441 cagttctacg gcaacccgga caacaaggcc tacatctacg ccgtccctta cgagccgccc
     4501 gccgagtcgc cggacaagga atatccgtac tggttcgtca ccggccgcgt gctcgagcac
     4561 tggcacaccg gcaccatgac gcgccgcgtg ccggagctgc accttgcctt cccgagcgca
     4621 gtcgcgttcc tccatcccga agacgccaag gctctcggcg tgcgccgcgg agacgagatc
     4681 gagatcgcat cccggcgcgg cgcgatgcgc agccgcgtgg aaacgcgcgg ccgcgtcaaa
     4741 gtaccgcgcg gccaggtcta cgtgccgttc ttcgacgcaa gccagctcgt caacaaggtg
     4801 acgctggacg cgaccgatcc gatctcgctc cagaccgatt tcaaaaaatg cgcctgcagg
     4861 atcagcaagg tatgagggcg gtttgccttc tcgcgctttt tctgctgctc ggcagcgcgt
     4921 tcgctcagca cgacccgcta cgcccggcgg ttccgttcga gcaggagccc aagaccgcgc
     4981 cgctcgtcaa cgccgagaac aacgaccgca agcgcgtgcg ctcctacgcg atgcagccgc
     5041 cgaccatccc gcacaagact gacaactacc aggtcgacaa gaacgcgaac aagtgcatga
     5101 cctgccacgc gcgcactcgc gccagcgaga cgcaggcaac gccgatcagc atctcgcact
     5161 accaggaccg tgacggcaac tacctcgccg acgtctcgcc gcggcgctac ttctgcgacc
     5221 agtgccacgt ggtgcaaatg cagaccgagc cgacggtgaa gaaccgcttc caggacgtgg
     5281 atacgctgat caagaaacag accaagaagt aatgagagaa ctgctgcgcc gctactggcg
     5341 gacgctcacg acgccgagca agttctacag cctcggcttt ctcaccatcg gcgggtttat
     5401 cggcggggtg ctgttctggg gcggattcaa caccgccatg gaattcacca acacagagca
     5461 gttctgcgtc tcatgccacg agatgcgcga caacgtctac caggagatcc agccgaccat
     5521 ccatttcacc aaccgctccg gcgtgcgcgc gatctgctcc gactgccacg tgccgcacaa
     5581 ctggaccttc aagatggcgc gcaagatgca ggcgtc
//
LOCUS       DASTPD010000283         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_560798, whole genome shotgun sequence.
ACCESSION   DASTPD010000283 DASTPD010000000
VERSION     DASTPD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560798"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFR57_09435"
     CDS             complement(<1..438)
                     /locus_tag="VFR57_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU0259625.1"
                     /translation="MDLRQQRASHNVEDRRGMGMAAGGIGVGGIVIALIAYFLGFDPT
                     AVMQQVGEPQAERPAAHGAPADETGQFVAKVLGSTEEVWTRVFAASGARYEPPTLVLY
                     EGQVRSACGMGQAAMGPFYCPGDRKLYIDLSFFRDLHTRFRAPG"
     gene            553..804
                     /locus_tag="VFR57_09440"
     CDS             553..804
                     /locus_tag="VFR57_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259626.1"
                     /translation="MGQPQAAVYARTLARAALLSGGPIALANKLGVRAAQMEAWLSGA
                     EEIPQATFLELVDVVMDAALGSLQEPSSSSPASVNPLQP"
     gene            854..1303
                     /locus_tag="VFR57_09445"
     CDS             854..1303
                     /locus_tag="VFR57_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEU0259627.1"
                     /translation="MFRAIVAIAAAFGFALGATAQEKDKSKDKQPTAQQERMKSCNAQ
                     ASKKELKGDERKAFMSKCLSAKGGDDKELTAQQKKMKSCNAQASKKDLKGDDRKKFMS
                     QCLKADAGDGRELTAQQERMKSCNTQANRRDLKGDKRREFMSDCLSA"
     gene            1426..1560
                     /locus_tag="VFR57_09450"
     CDS             1426..1560
                     /locus_tag="VFR57_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259628.1"
                     /translation="MLSPEPTEIGRGNNVLGEIFAKDVSSSVAERASAESGVQPSILK
                     "
     gene            complement(1683..1814)
                     /locus_tag="VFR57_09455"
     CDS             complement(1683..1814)
                     /locus_tag="VFR57_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259629.1"
                     /translation="MLPRAPDAVKAVAAQIFYAAWRSAVADPLYRKLMHEHRTRTAR"
     gene            complement(1880..2164)
                     /locus_tag="VFR57_09460"
                     /pseudo
     CDS             complement(1880..2164)
                     /locus_tag="VFR57_09460"
                     /inference="COORDINATES: protein motif:HMM:NF025686.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 family protein"
     gene            complement(2107..2232)
                     /locus_tag="VFR57_09465"
     CDS             complement(2107..2232)
                     /locus_tag="VFR57_09465"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU0259630.1"
                     /translation="MIREAAYLKAEARNFAPGVELNDWLEAEKEVLERLRASTTR"
     gene            complement(2353..2988)
                     /locus_tag="VFR57_09470"
     CDS             complement(2353..2988)
                     /locus_tag="VFR57_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0259631.1"
                     /translation="MKRGEIVYRADQRIKAVYFPEEALIAMVDTTEDRRTVEVGLIGR
                     EGIVGINIFLGGVVTPDRAIVQLPGGAFRMKSIDLRREVRFGSPLQRLLLNYTRTFLA
                     VISQSVACSQHHSVEQRLARWLLTMDDYAGAREFLMDHGSIAAMLGVRREGISEAAHR
                     LRKASLMDYRRGRVTILDRRGLQKRSCECYRFILREYGSLRGEVPRLLSRE"
     gene            complement(3087..>4009)
                     /locus_tag="VFR57_09475"
     CDS             complement(3087..>4009)
                     /locus_tag="VFR57_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0259632.1"
                     /translation="PIGWIIAGAVLLFAAGIVMAQRLGSRISRSIHGLMPLAVALGRG
                     DPVVVPSLRLQEADDVGRELVNASARLHDRERTLALVSHDLRSPLNVFMIGAARVERL
                     AQRLPGGEPICDVAASLTDLTRRMSGMVDDLLSIAVATGGGRSLLKIAPVSTASLLKR
                     AAAAAWPLFERGDIRLEVESVGALPELEVDSDRVLRVFANLIDNALKFTDPQGRVVLR
                     AETESDAMRYCVANSGPALSQAERESMFKPFWQAGHGDRRGAGLGLSICRSIVEAHGG
                     RIWAEPETGKRVRICFTLPLTGVSAPTAAV"
BASE COUNT          749 a         1307 c         1302 g          651 t
ORIGIN      
        1 gccaggcgcg cggaaccgcg tgtgcaggtc gcggaagaag gaaagatcaa tgtagagctt
       61 tcggtcgccg ggacagtaga acggtcccat ggccgcctgc cccatcccgc aggcggagcg
      121 cacttggcct tcatagagta cgagcgtcgg cggctcatac cgcgcgccgg aagccgcgaa
      181 gacgcgcgtc cacacctcct cggtgctgcc gaggaccttg gcgacgaact gcccggtctc
      241 gtccgcgggc gcgccgtgcg cggctgggcg ctcggcctgc ggctcgccaa cctgctgcat
      301 cacggccgtc ggatcaaagc ccaggaagta cgcgatcaga gcaatgacga tgccgccgac
      361 gccaatgccg ccggcggcca tccccatccc gcgccgatct tcaacgttgt gacttgcgcg
      421 ctgctgccga aggtccatgc gggcgtgagt gcaacagctg tgccgggacc tttgctgcgg
      481 cgcgatgtcg tgtccgagcg gcattgcgcg tcgtgacatt aaggcgcatc ctgcttcgga
      541 tcggagaagt gcatggggca gccgcaagcc gctgtttacg cccgcacctt ggcgcgtgcc
      601 gcgctgcttt ccggtgggcc gatcgcgttg gccaacaaac ttggcgtccg cgctgcgcag
      661 atggaggctt ggctgagcgg cgctgaagaa ataccgcaag cgacgtttct tgagctcgtg
      721 gacgtcgtca tggacgccgc gctcggctcg ctgcaagaac cttcgagctc ctcgcccgcg
      781 tcagtcaacc ccctacagcc gtagggactt tttcttgctt cccccagggg ccggccattc
      841 ttaggagacg accgtgtttc gagccatcgt agcgatcgcc gcagcgttcg gttttgcgtt
      901 aggcgcgaca gcgcaagaga aagacaaatc gaaggacaag cagccaacgg cacagcagga
      961 gcgcatgaag tcctgcaacg cccaggcaag caagaaggag ctgaaaggcg acgagcgcaa
     1021 ggccttcatg agcaagtgcc tgagcgcgaa aggcggtgac gataaggagc tcaccgcgca
     1081 gcagaagaag atgaagagct gcaatgccca ggcgagcaag aaagacctga agggcgacga
     1141 ccgcaagaag ttcatgagcc agtgcctcaa ggccgacgcc ggcgatgggc gcgagctcac
     1201 tgcgcagcag gaaaggatga agtcctgcaa cacgcaggcg aatcgccgcg acctgaaggg
     1261 cgacaagcga cgcgagttta tgagcgactg cttgagcgca taggaagcaa aatgcaaatc
     1321 acggacatcc tgggaagcag cggcggcctt cagaacatgg cgcggtgatg tcgatgggct
     1381 cgaaggcctc attggccgga tgggcggcgg ttctctgccg agaacgtgct atcgccggag
     1441 ccgaccgaga tcggtcgcgg caacaatgtg ctcggcgaga tcttcgccaa ggacgtgagc
     1501 agctcggttg ctgagcgcgc ctcagcagaa tcgggcgttc agccctccat cctcaagtag
     1561 acgctgccga tgatggcgat ggctgccgcg gcctacatgg cgcgtcagcg cggcgccatg
     1621 ggcggcggga tgttcgggga cggcaacccg ctcgacgaca tcatgcgcat tgtcgggcgg
     1681 cgctagcgcg ccgtgcgggt gcggtgctcg tgcatgagct tgcggtaaag cggatcggcc
     1741 accgcgctgc gccaggcggc gtagaaaatc tgcgccgcca ccgccttgac cgcatccggg
     1801 gcgcgaggca acaagcggcc cttgcatact tgcggccgct acggcggttc gcgtagcggc
     1861 gcgcgcgcgt gtaacccatc tgcaggaact tgcgcgccat gtccatgccg acgaagtcgc
     1921 gcttggcccc gtaggcgcga aagcgccgca ggatcgcagc gctcgactcg cgcgcaagct
     1981 cgggcgtgcg aaagcgccat agcggcagga tctcgctttt atagggctcg acgagcagca
     2041 cgccctgctc gccgcggcca atgcggtaga ggtccggccg cttgcgaaag tcgatgcggt
     2101 cgaaggctaa cgcgtagtcg aagcgcgcag ccgctccagg acttcctttt cggcttccag
     2161 ccaatcgttc aactcgacac caggcgcgaa gttgcgcgct tcagctttga ggtatgccgc
     2221 ctcccggatc attcggtaga tctcttcggc cgaaagctgc ttgggatcgg aatctggcat
     2281 acggcataga cgttcgctgc gaacaggggt tcccggtggg ctcgcgttcg cgcgcttggc
     2341 ttttctgcgg cgctactccc gcgacaaaag gcgcggcacc tcgcctcgca ggctcccgta
     2401 ttcacgcagg atgaaccgat agcactcgca cgatctcttc tgaaggcccc gcctgtcgag
     2461 aatggtgacg cggccgcggc ggtagtccat cagcgaggct ttccgcaacc tgtgcgcagc
     2521 ctcggaaata ccctcgcgac gcacccctag catggcggca atcgacccat ggtccatcag
     2581 aaactcgcgc gcccccgcat agtcgtccat ggtgagcagc cagcgcgcga ggcgctgctc
     2641 tacgctgtgg tgctggctgc atgccacaga ttggctgatc acggcgagaa acgtccgcgt
     2701 gtagttcagg agcagccgtt gcaacgggct gccgaaccgc acctctcgac gcagatcgat
     2761 cgacttcatc cggaaggcgc cgcccggcag ttgcacgatc gccctgtcag gggtgacgac
     2821 gccgccaaga aaaatattga tgccgacgat tccttcgcga ccgatcagtc cgacctcgac
     2881 ggtgcgccga tcttccgtgg tgtcgaccat ggcgataagc gcttcctccg ggaagtacac
     2941 cgccttgatt ctctgatccg cccggtagac gatctcgccg cgcttcaagc tgacgtgctt
     3001 gagcctcggg aggatgcgct tatattccgt ggcgaggagc gcggcgagga tgccgttcct
     3061 gacgggggta cgcgtcacgg tccgggtcac accgcagctg tgggcgcgct gacacctgtc
     3121 aatggcagcg tgaagcatat ccgcactcgc ttgccggtct ctggctccgc ccagatcctg
     3181 ccgccgtgcg cttcgacgat cgagcggcag atcgaaagcc cgagccccgc cccgcgccgg
     3241 tcgccatgcc cggcttgcca gaacggcttg aacatgcttt cccgctctgc ttgggaaagc
     3301 gccggcccgc tgttcgccac gcaatagcgc atcgcgtccg attccgtctc cgcgcgcagc
     3361 accacacgcc cctgcggatc cgtgaacttc agcgcgttgt cgatcaagtt cgcaaatacg
     3421 cgcagcacgc ggtccgaatc cacctcgagc tcgggaagcg cgccgaccga ttcgacctcc
     3481 agcctgatgt ccccgcgctc gaaaagcggc caggcggcgg ccgcagctcg cttcagcagc
     3541 gacgcggtgc tcacgggtgc gatcttgagc aacgaccggc cgccgccggt cgcaaccgcg
     3601 atcgagagca gatcgtccac catgccggac atgcgacgcg tcaggtcggt gagcgaggcg
     3661 gccacgtcac agatgggctc gcccccggga agccgctgcg ccaggcgttc gaccctggca
     3721 gcgccgatca tgaacacgtt cagagggctg cgcagatcgt ggctcacgag cgcgagcgtc
     3781 ctttctcgat cgtgcaagcg cgcggaggcg ttcacaagct cgcgtccgac gtcgtccgcc
     3841 tcctgcaggc gcaacgacgg gacgaccacc gggtcgccgc ggcccagcgc gacggccagc
     3901 ggcatcagac catgaatcga gcgggagatc cgggagccga gcctctgcgc cattacgatc
     3961 ccggccgcga acaataggac ggcgcctgcg atgatccacc cgataggaa
//
LOCUS       DASTPD010000284         4166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1174751, whole genome shotgun sequence.
ACCESSION   DASTPD010000284 DASTPD010000000
VERSION     DASTPD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174751"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="VFR57_09480"
     CDS             complement(<1..1755)
                     /locus_tag="VFR57_09480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0259633.1"
                     /translation="MASRREFLKGSVSAAALSGCSTLMPTQAADTLIVNGRIATLDPK
                     NPNAEAIAIRGERIVEVGNPGELEKYRGPNTRVIDAGRRIVIPGLNDAHTHFIRGGLT
                     YTNEVRWDGVPSLAEGMRRVREQAKRTPPPHWVQVIGGWTWAQFAEKRHPTLDEINAA
                     TGDTPCMIMHLYDRAWLNRSAVRVLGWEKEVPKLFGGFVERDATGRPTGLVMSTTSLA
                     SLVSVWLRVPRLSQDEQVISTRHFMREHNRLGVTSVIDAGGGGQNFPENYAAIAKLAA
                     DRRLTLRIGYQLFAQTPGKELENYQVWSKLVSIGQGDDYYRMVGAGEYLLYAAGDPAN
                     FAKDWVKPPAGAMEPQFAAVVKHLAALGWPFRQHATFDSTAARQLDVLEQVNREVPLK
                     KLRWGLDHCETLTPKSLERLAALGGSVSIQNRMSLDGEAFVAKYGVAAAEDAPPIARI
                     REMGIPLACGTDANRATSYNPWIGVHWLLTGKTLGGSKLQGEKNLLDRTEALRLFTAG
                     GAWISSEETKKGTLEAGKLADLVVLSGDYFSMPVDEVKDLESVLTMVGGKVVYAAAPY
                     AQLDAPPLPSLPDWLPVRH"
     gene            complement(1739..2653)
                     /locus_tag="VFR57_09485"
     CDS             complement(1739..2653)
                     /locus_tag="VFR57_09485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259634.1"
                     /translation="MQTRTSLGWIGTGRMGYEMAARLAKGGCDVSVWNRTREKAQPLE
                     KYGAKVVNQLEELSTKEILFVMVSTYDDVKEVIGKALAKGKPKMVVECSSISLEGSAE
                     LRKTLESKGIDYLAAPVSGNAKVIKAGKLTFVVSGPKLAYEKAKPYLDMMGTGSSYVG
                     EGELARIAKICHNVMLGVVIQNLCEITILAEKAGMPRHAFLDFLNKSVMGSMFTRYKT
                     PALVNLDFHVTFTPKLLRKDLDLGLEAGRKFDVPMPLSALTRDLLQQMIGHGMTEQDF
                     STLLLMQAKASGIELKPENKEVGDGLSS"
     gene            complement(2637..3938)
                     /locus_tag="VFR57_09490"
     CDS             complement(2637..3938)
                     /locus_tag="VFR57_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0259635.1"
                     /translation="MAKKTSRTKVARPDDVDPRHRWDRPLQAPGRTQVDFEERVDFKR
                     LHEYRLARTRAALAQSGLGALLCFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGKPY
                     IWDFGSAARHHKLYAPWLKTDHCRAGLLGMRGAVGSDLFLFEQAAKEIKAILKAEGVA
                     NMPLGVDVVEPPMLFALQKEGIEVRDGQQVMLEAREVKNIDELTLLNMAAAMVDGVYQ
                     DIAEALKPGVQESQIVALATKRLYEMGSDCVEAINSISGERCSPHPHNFTDRLIRPGD
                     QAFFDIIQSFMGYRTCYYRTFNVGRATDAQRTAYKKAREWMDRAIELIKPGMSSDKIA
                     LAMPEAKEIGFASEMDAFGLNFCHGLGLGLHERPLISRVNSLREPIELKAGMVFAVET
                     YCPATDGISAARIEEEVVLTPQGAKIISLYPAEELPIANPY"
     gene            complement(3951..>4166)
                     /locus_tag="VFR57_09495"
     CDS             complement(3951..>4166)
                     /locus_tag="VFR57_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259636.1"
                     /translation="SRLVTAAVELKKGSISPSTISTAVDAASAQGWRRPLLAWLGVQL
                     KRAEAAGDSETAARLRRRMALVSGPGS"
BASE COUNT          671 a         1398 c         1324 g          773 t
ORIGIN      
        1 gtggcgcacg ggcagccaat cgggcagcga tggcagcggc ggcgcatcga gctgcgcgta
       61 cggggccgcc gcgtacacca cctttccgcc caccatcgta agcaccgact cgagatcttt
      121 cacctcgtcc acgggcatgg agaaataatc accgctcaag acgacaaggt cggcgagctt
      181 gccggcctcg agcgtgcctt tcttcgtttc ttccgacgag atccacgcgc cgccggcggt
      241 gaagagacgc agcgcttcgg tgcggtcaag caggttcttc tcgccctgca gctttgaacc
      301 gccgagcgtc ttgccggtga gcagccagtg cacgccgatc cacgggttgt agctcgttgc
      361 gcgattcgcg tccgtgccgc aggcgagcgg gatgcccatc tcgcggatgc gcgcgatcgg
      421 cggcgcgtct tcggccgcgg cgacgccgta cttggcgacg aacgcttcgc catccagaga
      481 catgcggttt tggatgctca cgctcccgcc cagggcggcg agccgttcaa gcgatttcgg
      541 cgtcagcgtc tcgcagtggt cgagacccca gcgcagcttc ttgagcggta cctcgcgatt
      601 cacctgctcc agcacgtcga gctgccgcgc ggcggtcgaa tcgaaggtcg catgctgccg
      661 gaacggccag ccaagtgcgg caagatgctt caccaccgcc gcgaactgcg gctccatcgc
      721 tccggccggc ggcttcaccc agtccttggc gaaattcgca ggatcgcccg ccgcgtaaag
      781 aaggtattcg cccgcgccca ccatgcggta gtagtcgtca ccctgcccga tggagacgag
      841 cttcgaccac acctggtaat tctcgagctc cttgccgggc gtctgggcga aaagctggta
      901 gccgatgcgc agcgtgagcc ggcgatcggc ggcaagcttc gcgatcgcgg cgtagttttc
      961 ggggaagttc tggccgccgc cgccggcatc gatcacgctc gtcacgccga gccggttgtg
     1021 ctcgcgcatg aaatggcgcg tcgaaatgac ctgctcgtcc tgcgaaaggc gcggcacccg
     1081 cagccatacg gacaccaggc tcgcgaggct cgtggtggac atgacaagcc cggtgggtcg
     1141 gccagtcgcg tcgcgctcga cgaaaccgcc gaagagcttc ggcacctcct tttcccagcc
     1201 aagcacgcgc accgcgctgc gattgagcca cgcgcggtcg tagagatgca tgatcatgca
     1261 cggcgtatcg ccggtcgcgg cgttgatctc gtcgagcgtc ggatggcgct tttcggcgaa
     1321 ctgcgcccag gtccagccgc cgatcacttg cacccaatgc ggcggcggcg tgcgcttcgc
     1381 ctgctcgcgc acgcggcgca tcccctcggc gagcgacggc acgccgtccc agcgcacttc
     1441 gttggtgtag gtgagcccgc cgcggatgaa gtgcgtatgt gcgtcgttca gccccggaat
     1501 gacgatgcgc cgccccgcgt cgatgacgcg cgtattcggg ccgcgatact tctcgagctc
     1561 gccgggattg cctacctcaa cgatgcgctc gccgcggata gcgatcgcct cggcgttagg
     1621 gtttttcggg tcgagcgtcg caatgcgccc gttgacgatt agcgtgtcgg ccgcttgcgt
     1681 aggcatgagc gtgctgcaac ccgaaagcgc ggcggcgctt acgcttccct tgagaaactc
     1741 acgacgagag gccatcccct acctccttgt tttcgggctt gagctcgatg ccggatgctt
     1801 tcgcctgcat aaggagcagt gtcgagaagt cctgctccgt catgccgtgg ccgatcatct
     1861 gctgcaagag atcgcgcgta agcgcgctga gcggcatcgg cacgtcgaat ttgcggccgg
     1921 cctcgaggcc gagatcgaga tccttgcgca gcagcttggg cgtgaaggtg acatggaaat
     1981 cgaggttcac gagcgccggc gtcttgtagc gcgtgaacat ggagcccatc acgctcttat
     2041 tcaggaagtc gagaaacgcg tgccgcggca tacccgcctt ctcggccagg atcgtgatct
     2101 cgcacaggtt ctgaatcacc acgcccagca tgacgttgtg gcagatcttc gcgatgcgcg
     2161 cgagctcgcc ctcgccaacg tagctcgagc ccgtgcccat catgtcgagg taaggcttcg
     2221 ccttctcgta ggccaacttc ggcccggaca ccacgaacgt gagcttcccc gccttgatca
     2281 ccttcgcgtt gccgctgacc ggcgcagcga gataatcaat gcccttgctc tccagcgtct
     2341 tgcgcagctc cgcggagcct tcaagcgaga tcgaggaaca ttcgaccacc atcttcggct
     2401 tacccttcgc cagggccttg ccgatcactt ccttcacgtc gtcataggtg gacaccatga
     2461 cgaagaggat ctccttcgtc gataactcct cgagctggtt gaccaccttc gcgccgtact
     2521 tctcgagcgg ctgcgccttt tccctagtgc ggttccagac cgagacgtcg caaccgccct
     2581 tcgcgagccg cgcggccatc tcgtagccca tgcggccggt gccgatccag ccgagactag
     2641 tacgggtttg caatcggtag ttcctctgcg gggtaaagcg agatgatttt ggcgccctgc
     2701 ggcgtgagga ccacctcttc ctcgatccgc gccgcggaga tcccgtcggt cgccgggcag
     2761 taggtctcaa ccgcgaatac catgcccgcc ttgagctcga tcggctcgcg cagcgagttg
     2821 acgcgcgaga taagtgggcg ctcgtgcagc ccgaggccaa ggccgtggca gaagttcagg
     2881 ccgaaggcgt ccatctcgct cgcgaatccg atctccttcg cttccggcat cgccagcgcg
     2941 atcttgtcgc tcgacatgcc gggcttgatc agctcgatcg cgcggtccat ccactcgcgc
     3001 gccttcttgt aggcggtgcg ttgcgcgtcc gtcgcgcgcc cgacattgaa cgtgcggtag
     3061 tagcacgtgc gataacccat gaacgactgg atgatgtcga agaacgcctg gtcgcctggg
     3121 cggatcaggc gatcggtgaa gttatgcggg tgcgggctgc agcgctcgcc ggagatcgag
     3181 ttgatcgcct cgacgcagtc ggagcccatc tcgtacaggc gcttggtggc gagcgcgacg
     3241 atctgcgatt cctgcacgcc cggcttgagc gcctccgcga tgtcctggta gacgccgtcg
     3301 accatcgccg cagccatgtt gaggagcgtg agctcgtcga tgttcttgac ctcgcgcgct
     3361 tcgagcatga cctgctgccc gtcgcgcacc tcgatacctt ctttctgcag cgcgaagagc
     3421 atcggcggct cgaccacgtc gacgccgagc ggcatgttgg ccacgccctc ggccttcagg
     3481 atcgccttga tctcctttgc cgcctgctcg aacaggaaca ggtcggaacc gaccgcgccg
     3541 cgcatgccga gcaagccggc gcggcaatgg tcggtcttca gccacggcgc gtagagcttg
     3601 tggtggcgcg cggcgctgcc gaagtcccag atgtagggct tgccgttgcc ggtgaggagc
     3661 gaatagcgcg tgagcttgtc gcgcgcccac tcgccgatca ccgtgctcgt ggtgtagcgg
     3721 atgttgtgct ggtcgaagca cagcagcgcg ccgaggcccg actgcgcgag cgccgcgcgc
     3781 gtgcgggcca gccggtactc gtgcaaccgc ttgaaatcga cgcgctcttc gaaatcgacc
     3841 tgggtgcgtc ccggcgcctg cagcggccgg tcccagcgat gccggggatc tacgtcgtcg
     3901 ggccgggcga ccttggttcg tgaggttttc ttggccatgg ccgggccatc ttaactcccc
     3961 ggcccggaca caagcgccat gcgccgccgc aggcgggccg ccgtttcgct gtccccggcc
     4021 gcctcggcgc gcttgagctg cacccccagc cacgccagga gcggccgccg ccacccttgc
     4081 gcagacgccg catcgacggc agttgaaatc gtggaaggac ttatggagcc cttcttcagc
     4141 tccaccgccg ccgtgacgag acgcga
//
LOCUS       DASTPD010000285         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_356790, whole genome shotgun sequence.
ACCESSION   DASTPD010000285 DASTPD010000000
VERSION     DASTPD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356790"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFR57_09500"
     CDS             complement(<1..871)
                     /locus_tag="VFR57_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259637.1"
                     /translation="MRKILLAWVLFTAANALAQPYPSKPIRVAVAFPPGGPVDIIARL
                     MGPKLGELLGQSIVVENVVGAGGNVAAVRVAKAAPDGYTVLAHSSAYAVNPTLIPNAG
                     YDGEKDFIPLAIVASQPNLIVVHADFPAKTLAELLERARTGKLAFASAGNGTTPHLTG
                     ENLFKIRAKVDITHIPFKGGGPAAAAVLSGQPPIGSIAGSAPLPHIKAGKLRALAVSS
                     AQRLAALPDVPTLGELGFSGMEDYTWVGFFVPAGTPAEVAQKLNDALLKVARDPQIKE
                     RLEALAFDVTAAPL"
     gene            complement(880..1851)
                     /locus_tag="VFR57_09505"
     CDS             complement(880..1851)
                     /locus_tag="VFR57_09505"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0259638.1"
                     /translation="MTERKLTRDDLRKAAEKYKNWGRWGPDDELGTLNHTRPEDIVAA
                     ARLVRKGKVISLALNFDNHGPQGAKSKYPALGRINPLHTMLRTGTDAYAGVLDKRGIR
                     AADDMVVMPLQCGTQWDGLGHVFYENHMWNGYDCREVTSAGAQKCGIEKTKNRMVGRG
                     VFLDVPRALGMDVLPDGYAITSRDLDKTALVEEVELRRGDFVIVRTGQMEAKLDAASW
                     DGYPGGDAPGFSFETLDWIQRTELAALASDTWGCEVRPNESEPGINQPWHWITIPIMG
                     MTMGEIFYVKDLAEDCAADGVYEFLFVAPALPITGAVGSPTNPLAIK"
     gene            1946..>2624
                     /locus_tag="VFR57_09510"
     CDS             1946..>2624
                     /locus_tag="VFR57_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216010.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0259639.1"
                     /translation="MANLEERRIASGFGFLAREAGFEIGESAFLRYSASDSYLRALVV
                     GLTNTLAVALIGIVTATVLGTAIGLSRLSRNFLLRNVAAAYVEFMRNVPLLVQLFFWY
                     AVITEGMPGPREALSPLPGVFLSNRGIFFPTLAGAPELQGFNFVGGAALTPEFVALLV
                     GLATYTAAFIAEIVRAGILAVDRGQAEAAHALGLSRAQAMRLVILPQALRVIVPPMTS
                     QYLNLTKN"
BASE COUNT          389 a          867 c          866 g          502 t
ORIGIN      
        1 cgagcggcgc cgcagttacg tcgaaggcta gcgcttccag ccgttccttg atctgcggat
       61 cgcgcgccac cttcagcagc gcgtcgttca gcttttgtgc gacttccgcc ggggtgccgg
      121 cagggacgaa gaatccgacc cacgtgtagt cctccatccc ggagaatcca agctcgccga
      181 gcgtcggcac gtcgggcaac gcggcgaggc gctgtgcgga agacacggcg agcgcccgca
      241 gctttccggc cttgatgtgc gggagcggcg ccgagcccgc gatcgagccg atcggcggct
      301 gtccgctcag gacagccgct gcggccggtc cgccgccctt gaagggaatg tgggtgatat
      361 cgaccttggc gcgtatcttg aacaggtttt cgccggtgag atgcggcgtg gtgccgttgc
      421 ccgccgaggc gaacgcgagc ttcccggtgc gcgcacgctc gagcagctcg gcgagcgtct
      481 tcgccggaaa gtccgcgtgc acgacgatca ggttgggctg cgaggcgacg atggcgagcg
      541 ggatgaaatc cttctcgccg tcgtatccgg cattcgggat gagcgtcgga ttcaccgcgt
      601 aagcagacga atgcgcgagc acggtgtagc cgtcgggcgc cgctttcgcg acgcgcacag
      661 cggccacgtt gccgccggcg cccaccacgt tctcgacgac gatgctctgt cccagaagct
      721 cgcccagctt cggccccatg agccgcgcga tgatgtctac cggcccgccc ggcgggaatg
      781 ccactgcgac gcggatcggc ttcgacggat agggctgtgc cagcgcattt gccgccgtga
      841 agagcaccca ggccagaaga atctttctca tcgtcccccc tacttgatgg cgagcggatt
      901 cgtcggtgag cctaccgcac cggtgatcgg aagggcaggg gcgacgaaca gaaactcgta
      961 aaccccgtcg gcggcgcagt cctcggccag gtcctttacg tagaagatct cgcccatcgt
     1021 catgcccatg atggggatgg tgatccagtg ccacggctga ttgattcccg gctcggattc
     1081 gttcggccgc acctcgcagc cccaggtatc gctcgccagc gccgcgagct cggtgcgctg
     1141 gatccagtcg agcgtctcga acgaaaagcc cggcgcgtcg ccgccggggt agccgtccca
     1201 gctcgcggcg tcgagcttgg cctccatctg cccggttcgc acgatcacga agtcgccgcg
     1261 gcgcagttcg acttcttcaa cgagagctgt cttatccagg tccctgcttg taattgcata
     1321 cccatccggc aggacgtcca tgccaagggc gcgcggtacg tcgaggaata cgccgcggcc
     1381 gaccatcctg ttcttcgtct tctcgatgcc gcatttctgc gcaccggcgg aggtgacctc
     1441 gcggcagtcg tagccgttcc acatgtggtt ctcatagaac acgtgaccca ggccgtccca
     1501 ctgcgtgccg cactgcagcg gcatcaccac catgtcgtcc gcggcacgga tgccgcgctt
     1561 gtcgagcacg cctgcgtagg cgtcggtgcc ggtgcgtagc atcgtgtgca gcgggttgat
     1621 gcgcccaagg gctggatact tgcttttcgc gccctgcgga ccgtgattgt cgaagttgag
     1681 cgcgagcgag atcaccttgc cttttctcac caggcgcgct gcggcgacga tgtcctcggg
     1741 ccgcgtgtga ttcagggtgc cgagctcgtc gtcgggcccc cagcggcccc agttcttgta
     1801 cttctccgcg gcttttcgca gatcgtctcg ggtaagcttg cgctcggtca tggtctggcg
     1861 ctcttcgagg gggaggcgga tagcgtatca ggcggcggcg atcggcgccg ccgtgctcgt
     1921 cgcctggttt ctcgtctcca acaccatggc gaacctcgaa gagcgccgca tcgcgagcgg
     1981 cttcggcttc ctcgcgcgcg aggcgggctt cgagatcggc gagtcggcgt tcctgcgcta
     2041 cagcgcctcg gacagctatc tgcgcgcgct cgtcgtcggg cttaccaaca cgctggcggt
     2101 cgcgctgatt ggcatcgtca ccgccaccgt gctcggcacg gcgatcgggc tctcgcggct
     2161 gtcgaggaac tttctgctga gaaacgtggc cgcggcgtac gtcgagttca tgcgcaacgt
     2221 gccgctgctg gtgcagctct ttttctggta cgcggtgatc accgagggaa tgccgggtcc
     2281 gcgcgaggcg ctgtctccgc tgcccggagt gttcctctcc aatcgcggga tcttctttcc
     2341 cacgttggcc ggcgcgcctg agctgcaggg tttcaatttt gtcggcggcg cggcgctcac
     2401 cccggagttc gtcgcgctcc tggtcgggct cgcaacctat accgcggcgt ttatcgccga
     2461 aatcgtgcgt gcgggcatcc tggcagtgga tcgcggccag gccgaagcgg cgcacgcgct
     2521 ggggctttcg cgtgcgcagg caatgcgcct ggtgattctg ccgcaggcgc tgcgcgtcat
     2581 cgtgcctccc atgacgagcc agtacctgaa cctgaccaag aaca
//
LOCUS       DASTPD010000286         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_766594, whole genome shotgun sequence.
ACCESSION   DASTPD010000286 DASTPD010000000
VERSION     DASTPD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766594"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFR57_09515"
     CDS             complement(<1..467)
                     /locus_tag="VFR57_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0259640.1"
                     /translation="MRMYDLPDAHGHFGPYGGVFVAETLTAALDELLAAYERARKDPA
                     FQAEFRYELKHYVGRPSPVYHARRLTECCGGAQIYVKREDLNHTGAHKINNTIGQALL
                     ARNMKKPRVIAETGAGMHGVATATVAARYGMECVVYMGSEDVKRQAANVHRMGV"
     gene            complement(454..1095)
                     /locus_tag="VFR57_09520"
     CDS             complement(454..1095)
                     /locus_tag="VFR57_09520"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU0259641.1"
                     /translation="MGTARVKTRVKICGITRSADAAAAAQAGVDAIGLVFYPPSPRFL
                     SAERAREIRDALPPFVQTVALFVNADAASVAQVIGRVHPAMLQFHGDETPDFCAQFGL
                     PYVKAYRTKSGATPGAALEYLRPFSRAAAWLFDSHVPEYGGVGESFDWSLVPATDKPV
                     ILSGGLSQANVAAAIRRVRPWGVDVSSGVESAKGIKDAAKIAAFIAEVRNADV"
     gene            complement(1077..1850)
                     /gene="truA"
                     /locus_tag="VFR57_09525"
     CDS             complement(1077..1850)
                     /gene="truA"
                     /locus_tag="VFR57_09525"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU0259642.1"
                     /translation="MRVALARRIALGLEYDGSRFLGWQTQPGGGTVQDALEEALSAIA
                     DTRVSTTAAGRTDRGVHARLQVVHFETEAKRPVSAWVRGVNALLPGSAAVLWSHEVSE
                     EFHARYSARSRTYRYALLNRAVRPALSAHYAGWFHRRLDVQRMREAAALLVGQHDFSA
                     FRSSECQAKTPVRTLHALEVEKNGETIEFLVRANAFLHHMVRNLVGTLVYVGNGKHPP
                     QWISEVLNSRDRAKAAPTFGPEGLYLETIEYEPRWGLPV"
     gene            complement(1860..4454)
                     /locus_tag="VFR57_09530"
     CDS             complement(1860..4454)
                     /locus_tag="VFR57_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEU0259643.1"
                     /translation="MAFAAPIAVHAAGLGRLTVLSPLGQPLNAEIEIVALRAGEEDTL
                     SARIAPLEAFEAAGIEPSAVLNTVRFAVERRDSRRILRVTTTQPVNEPFVELLVELQW
                     HNGRLVREYTFLLDPPEYKPRDALAVPRAPKPAKPAAEVEAKPVEPAAPAPPPLAVAP
                     APAPTPASEKPAPAAEPEKPATVAQSEKPAADVEVVKEPAKEESPQVLADEPAVYEVV
                     QGDTLGKIARDKLPPGITLNQMLVAIYRANQDAFIRENLNLVRAGRILNIPGSDAVGT
                     VDVEEANRVVRAHMAEFREYRGRLAAAPAVADAASGQREAAGQIEAKPQAPKPAAAED
                     QVRLSKVEPQKPSLAAAAAARGDDLAAQDRALKEAQSRIGDLEKNVADLQKLLELKNQ
                     QLAELEKRSGAKPAAAPSAPAAASAPAAKGPEAPQPAPAASSAPTPVAQAPEASTPAV
                     QAPAGSQPAAATPVAKAAPKKMAPPPSEPSLLDEFLENPVALAGLAGVVVLLVGYAAW
                     AWRKKKTAQVKFQDSVIGAAAAGAAAGASISEPSISGARPMSSQASSGSPPAAMAGEE
                     VDPIAEADVYMAYGRDAQAEEILKEALQKDAKRVAVHAKLLEIYAKRKDTKAFERSAA
                     KLKNLTGGSGPEWQKAAALGKSIDPSNGLYAGAAAAAAAAAAAEVSAPSPSSAPALDF
                     DVGSVTQVHAAVPDFTLDLPAQKAAANTVLDFDLSGTTTKKDGFTEDTVVPASAVDKN
                     NGLDFDLDLGTPAAPGAKPEPAATAPAVDPGLSFDLDLEKIDSAPAAVTATDLSTISL
                     DLGSAGSASAAGTDPKWQEIATKLDLAKAYEEMGDKDGARELLNEVMKDGDAAQKGQA
                     QQLLVKLG"
     gene            complement(4590..>4799)
                     /locus_tag="VFR57_09535"
     CDS             complement(4590..>4799)
                     /locus_tag="VFR57_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEU0259644.1"
                     /translation="VPNRREESLAELTPAAVTGKLTVPVGRLRKLPMGEDYLAAFSVG
                     DQLLWGAAEPLRRMVRILLDAGVKP"
BASE COUNT          737 a         1623 c         1560 g          879 t
ORIGIN      
        1 acgcccatgc ggtgcacgtt cgccgcctgg cgcttgacgt cctccgagcc catatacaca
       61 acgcactcca tcccgtagcg cgcggccacg gtcgcggtgg caacgccatg catgccggcg
      121 ccggtctcgg cgatcaccct gggcttcttc atgttgcgcg cgagcagcgc ctggccgatg
      181 gtgttgttga tcttgtgggc gccggtgtgg ttcaaatcct cgcgcttgac gtagatctgc
      241 gccccgccgc agcattcggt gagccgcctg gcgtggtaaa ccgggctcgg ccggccgacg
      301 taatgcttga gctcgtagcg gaactcggcc tggaacgcag gatccttgcg cgcgcgctcg
      361 taggcggcga ggagctcatc gagcgccgcg gtgagcgtct cggcgacgaa cacgccaccg
      421 tatggaccga agtgcccgtg cgcatcgggc aagtcataca tccgcattcc tgacctctgc
      481 aatgaaggcg gcgatcttcg ccgcgtcctt gatccctttc gccgactcca cgccgctcga
      541 gacgtccacg ccccagggac gcacgcgccg gattgccgcc gcgacgttgg cttgcgacag
      601 tccgccggac aggatcaccg gtttgtcagt ggcgggaacc agcgaccaat cgaagctttc
      661 gccaacgccg ccgtactccg gaacatggct gtcgaacaac caggccgccg cgcgcgagaa
      721 tgggcgcagg tattctagcg cagcccccgg cgtagccccg cttttcgtcc tgtatgcctt
      781 gacgtacggc agcccgaact gcgcgcagaa atccggtgtc tcgtcgccgt ggaactgcag
      841 catcgcggga tgcactcggc cgatcacctg ggctaccgac gcggcgtccg cgttgacgaa
      901 aagcgccacc gtctgcacga acggcggcag cgcgtcgcgg atctctcgcg cgcgctcggc
      961 cgagaggaag cgcgggctcg ggggataaaa cacgagcccg atcgcgtcca cgcccgcttg
     1021 cgccgcggcg gcggcgtcag cgctgcgcgt gatgccgcaa atcttgacgc gcgtcttcac
     1081 acgggcagtc cccatctggg ctcgtactcg attgtctcaa ggtacaggcc ttccgggccg
     1141 aacgttggcg ccgccttggc gcgatctcgc gagttcagca cctcgcttat ccattgcggc
     1201 ggatgcttgc cgttgccgac gtacacaagc gtccccacga gattgcgcac catgtgatgc
     1261 aggaacgcat tcgcgcgcac caggaactcg atcgtttcgc cgttcttctc gacctcaagg
     1321 gcatggagcg tgcggaccgg cgtcttggcc tggcactcgg aagagcgaaa cgcggaaaaa
     1381 tcgtgctggc cgacgagcag cgcggccgcc tcacgcatcc tttgcacatc gagccgccga
     1441 tgaaaccacc cggcgtaatg cgccgacagc gccgggcgca ccgcgcggtt cagcaatgca
     1501 taccgatagg tgcgggagcg cgcgctgtag cgcgcatgga actcttcgga cacctcgtgc
     1561 gaccagagca ccgcagccga gccgggaagc aacgcgttca cgccccgcac ccaggccgaa
     1621 actggacgct ttgcctccgt ctcgaagtgg acgacttgaa ggcgtgcatg cacgccgcga
     1681 tcggtgcgcc cggcagccgt ggtggacacg cgcgtatcgg cgatcgcgga gagcgcctcc
     1741 tcgagcgcat cttgtaccgt cccgccgccg ggctgagtct gccagccaag gaagcgacta
     1801 ccgtcgtact ccagccctag agctatcctt cgtgccagag ctaccctgat gacggcgcgt
     1861 tagcccagct tcacgagcag ctgctgcgcc tgccctttct gcgcggcgtc gccatccttc
     1921 atcacttcat tcaacagttc gcgcgcgcca tccttgtccc ccatttcctc gtatgccttg
     1981 gcaagatcga gcttggtggc gatctcctgc catttggggt cggtgccggc ggcagaggcg
     2041 ctgccggcgg agccgaggtc cagactgatg gtcgagagat ccgtcgccgt caccgcagcc
     2101 ggagcggagt ctattttctc gagatcgagg tcgaagctca ggccggggtc gacggccggg
     2161 gcggtagcgg ccggctcggg ctttgcaccg ggcgcggcgg gcgttccgag atcgaggtcg
     2221 aagtcgagtc cgttgttctt gtccaccgcg ctcgccggga ctaccgtgtc ctctgtgaat
     2281 ccgtcctttt tcgtggtcgt cccgctgaga tcgaagtcga gcacagtatt ggcggccgcc
     2341 ttttgcgccg gcaagtcgag cgtaaagtcc ggcaccgcgg cgtgcacctg cgtcacgctg
     2401 cctacgtcga aatcgagcgc gggcgctgac gacggtgacg gtgccgatac ctcagcggcc
     2461 gccgcagccg ccgcagcagc agcagcaccg gcgtacagac cgttgcttgg atcgatcgac
     2521 ttgccgagcg ctgctgcctt ttgccattcg gggccgctgc ccccggtcag gttcttgagc
     2581 ttggccgcgc tccgctcgaa agcctttgtg tccttgcgct tggcatagat ctcaaggagc
     2641 ttcgcgtgca ccgcgactcg ctttgcgtcc ttctgcagcg cctctttgag aatctcctcg
     2701 gcctgcgcat cgcggccgta ggccatgtag acatcggcct cggcaatcgg atcgacttcc
     2761 tcgcccgcca ttgcggcggg cggcgaaccg cttgaggcct gtgaagacat gggccttgcg
     2821 ccactgatgc tcggctcgct gatgctcgcg ccggccgccg cgcctgcggc cgcggcgcca
     2881 atcacgctat cctggaactt gacttgtgcg gtcttttttt ttctccatgc ccaggccgcg
     2941 tacccgacca gcagcactac cacaccggca agcccggcaa gggccaccgg attctcgaga
     3001 aactcgtcga gcaggcttgg ttcagatggg ggcggcgcca tcttcttggg cgccgccttg
     3061 gctaccggcg tggctgcggc aggctgcgac cccgcaggcg cctggacagc cggagtggat
     3121 gcctccggag cttgcgctac aggggtcgga gctgaagatg ccgccggcgc cggctggggc
     3181 gcctcaggtc ccttggcggc cggagccgat gcagctgccg gcgctgaagg cgcggcggcg
     3241 ggcttcgcgc cagatctctt ttcgagctcg gcgagctgct gattcttcag ctcgagaagc
     3301 ttctgcaggt cggcgacgtt cttctcgagg tcgccaatgc gcgactgcgc ttccttcagc
     3361 gccctgtcct gtgccgctag gtcgtccccg cgcgccgctg ctgcggcggc gagcgaaggc
     3421 ttctgcggct cgaccttgga gagacgcact tggtcctcgg ccgcggcggg cttcggtgcc
     3481 tgcggcttag cctcgatctg tccggccgcc tcgcgctgtc cggaagccgc gtcggcaacg
     3541 gctggcgcgg ctgcgagacg gccgcgatac tcgcggaact ccgccatatg cgcgcgcaca
     3601 acgcgattcg cctcttctac gtctacggtg ccgaccgcgt ccgagcccgg aatgttcaat
     3661 atgcgcccgg cgcgcaccag gttcaggttc tcgcgaatga aggcgtcctg gttagcgcga
     3721 tagatcgcca ccagcatctg gttgagcgtg atcccgggcg gcagcttgtc gcgggcgatc
     3781 ttgccgagcg tgtcgccctg cacgacctcg tagacggccg gctcgtcggc aagtacctgc
     3841 ggcgactcct ctttggcggg ctccttcacg acctctacgt ccgccgcggg cttctcggac
     3901 tgtgcgacgg ttgcaggttt ctctggctct gccgcgggcg cgggcttttc cgatgctggg
     3961 gttggagccg gcgctggcgc gacagcgagc ggtggcggcg cgggcgcagc cggctcgacg
     4021 ggctttgcct ccacttctgc ggccggcttc gccggctttg gcgcccgcgg cacagcgagc
     4081 gcgtcgcgcg gcttgtattc gggaggatcg agcaggaaag tgtattcacg caccaggcgc
     4141 ccgttgtgcc attgcagctc gacgagcagt tcgacgaacg gctcgttgac cggctgcgtt
     4201 gtcgtcacgc gcaagatgcg tctgctgtcg cggcgctcga cggcgaagcg caccgtgttc
     4261 aacaccgcgc ttggctcgat gccggcagcc tcgaaggcct cgagcggcgc tatgcgcgcg
     4321 gagagcgtgt cttcctctcc ggctcgaagc gcgacgattt cgatctcagc gttcaaaggc
     4381 tggccgagcg gcgacagaac ggtgagcctg cccaggccgg cggcgtgaac ggcaataggc
     4441 gccgcgaagg ccaacgcggc gacgacagca actacctttg aagttatccc tctacccact
     4501 ggctgcccat cccccggagt gttcttcgca ggaccaacta ccttaacatc atgggcttag
     4561 gcgtgcaaat ggagaaagtt tctaaattcc taaggcttca cgcctgcgtc caggagaatt
     4621 cgcaccatgc gccgaagggg ctcggcggcc ccccaaagca gctggtctcc cacggaaaaa
     4681 gccgccaaat agtcctctcc catagggagc ttgcgtagac gccccaccgg gacggtcagc
     4741 ttgccggtga cggcagccgg ggtcagctcg gcgagcgatt cttcgcgccg gttcgggac
//
LOCUS       DASTPD010000287         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1378400, whole genome shotgun sequence.
ACCESSION   DASTPD010000287 DASTPD010000000
VERSION     DASTPD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378400"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..864)
                     /locus_tag="VFR57_09540"
     CDS             complement(115..864)
                     /locus_tag="VFR57_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0259645.1"
                     /translation="MRVVITGASSGIGEALARHYARDDVVLGLISRRKADPKGLPGEL
                     ISYAADVADEAALAAAAQDFMRRFGPPDLVIANAGIGTGTEAAEIRHVAKLRKILETN
                     VVGLAATLAAFAPAMRAAGGGTLAGIASVAGFRGLAGNGAYCASKSAAITWMESLRAE
                     LHGSGVSVVCVCPGYIDTPMTRVNRFRMPFLLSADEAARRIARAIAAKRRLAVIPWQM
                     ALVSLLLRAMPAWLFDRLASRAPRKPKDIPV"
     gene            complement(881..1480)
                     /locus_tag="VFR57_09545"
     CDS             complement(881..1480)
                     /locus_tag="VFR57_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEU0259646.1"
                     /translation="MNRVRRALVATISLAPLAALAQRTDYVELNPPQPVETTDKIEVL
                     EFFWYGCIHCYNLEPKLETWLKTLPKDVEFRRVPAVFNERWAHDASIYYAFEAMGLLD
                     KLHRPFFDSIHRDRLRTDNWQNLAAWLEKQGVDTKKFETTFKSFGVQSKTKRAIRLTT
                     GYKIDGTPAMAVHGRYTVPSSEGMLGTVNQLVAQVRKQK"
     gene            complement(1477..2082)
                     /locus_tag="VFR57_09550"
     CDS             complement(1477..2082)
                     /locus_tag="VFR57_09550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0259647.1"
                     /translation="MPMSRSMTRKPRKREGGNFLAGIGVGVLVGLAVALGIAFYLNRT
                     PIPFTTAKPKQPEKAAASGKPPAITGLPQSAAPAPIASVEKPKFDFYRILPGSEEPVT
                     EKEIRERMRAKGGQQDSGKDVYFIQAGSFQNPAEADNQKARLAILGFDSSVEPANLPD
                     KGTWYRVRMGPYAKLDDINKVRQALAQNGINASLIKVKEQQ"
     gene            complement(2083..3723)
                     /gene="argS"
                     /locus_tag="VFR57_09555"
     CDS             complement(2083..3723)
                     /gene="argS"
                     /locus_tag="VFR57_09555"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU0259648.1"
                     /translation="MTSDPKARLAEAYRQALAKEAPDHPELEVVFEKPRQPEHGDFAC
                     SIALQLAKRLKRNPRQLAEALTFKALAAFPDFDKFFAGCEIAGPGFINFRLRPEVKLA
                     AVRSALAAGADWGRATKDRPLSVQVEFVSANPTGPLHVGHGRQAAIGDALAAILEARG
                     DKVTREFYYNDAGAQIHNLALSVQARARGLKPGDAGWPADGYAGEYIEEIAKQYRGSL
                     EDLEPIRRFAVDCLRREQDVDLREFGVKFDVYYLESSLYAEGKVDEVVNALIASGQTY
                     EKDGALWLRSTKYGDDKDRVIRKSDDTYTYFVPDVAYHLTKWRRGFAKVINVQGTDHM
                     GTKARVRAGLQALNVGVPPGYPDYVMHSMVRVMRGGQEVKISKRAGSYVTLRDLLDWV
                     GRDAVRFFLLSRRADADFVFDIDLARAQTEENPVYYVQYAHARVCSVFGQWKGNPAEL
                     SAADLSRLTNEKEFALARRIGEFSDLVAEAARELSPHSITFYLRSLAAEFHSYYNAER
                     ILVDDEALRIARLALCAAVRQTLASGLSLIGVSAPEKM"
BASE COUNT          637 a         1205 c         1229 g          701 t
ORIGIN      
        1 cacgcctctt gaggcactga aggcgactgt tgcgtcaacg ggttgaaatc acccagagcc
       61 cgatttccca gagcgggagc ggaagtaacg gaaatcgggc tctgaccccg atttctagac
      121 cggaatgtcc ttgggcttgc gaggggccct ggacgcaagg cggtcgaaga gccatgccgg
      181 catcgcacgc agtagaagcg aaaccagcgc catctgccac ggaatcaccg cgagtcgtcg
      241 tttggccgcg atcgcgcgcg cgatgcgccg cgcggcttca tcggccgaga gcaggaacgg
      301 catccggaag cggttgacgc gggtcatcgg cgtgtcgatg tagcccgggc agacgcatac
      361 aacggaaacg ccgcttccgt gcagctcggc gcgcaagctc tccatccaag tgatcgcggc
      421 cgatttcgaa gcgcagtagg cgccattgcc agcgaggccg cggaagcccg cgacgctcgc
      481 gattccggca agcgtgccac cgccggcggc gcgcatcgct ggggcgaaag ccgcgagcgt
      541 cgccgcaagg ccgacgacgt tggtttcgag gatcttcctt agcttcgcca cgtggcggat
      601 ctccgcggcc tcggttccgg tcccgatccc ggcgttggcg atcacgagat ccggaggccc
      661 gaagcggcgc atgaaatcct gcgcggcagc ggctagcgcc gcctcgtcgg ccacgtcggc
      721 ggcataggag atgagctcgc cgggaaggcc cttgggatcg gctttgcggc gagaaatgag
      781 gccaagaaca acgtcgtcgc gcgcgtagtg ccgcgccagc gcctctccga taccggagga
      841 ggcgccggtg atgacgaccc gcacgttgtc gcgacctacc ttatttctgt ttgcggacct
      901 gcgcgaccag ctggttgacc gtgccgagca ttccctcgct cgagggcacg gtatagcgcc
      961 catgcactgc catggccggc gtgccgtcaa tcttgtagcc ggtggtgagg cggatggcgc
     1021 gcttcgtctt gctttgcacg ccgaacgatt tgaacgtggt ttcgaacttc ttggtgtcca
     1081 cgccctgttt ctcgagccac gcggccaagt tctgccagtt atcggtgcgc agccggtcgc
     1141 ggtggatcga atcgaagaac ggccggtgca gcttgtcgag cagtcccatc gcctcgaagg
     1201 catagtaaat ggaggcgtcg tgcgcccagc gctcgttgaa caccgcgggc acgcggcgga
     1261 attccacgtc cttcggcagc gtcttcagcc atgtctcgag cttgggctcg aggttgtagc
     1321 agtgaatgca tccgtaccag aagaactcga gtacctcgat cttgtccgtg gtctcgaccg
     1381 gctgcggcgg gttgagctcg acgtagtcag tacgctgcgc gagcgcggcg agcggggcca
     1441 gggagattgt cgcgacaagc gcgcggcgta ctcgattcat tgctgttcct ttactttgat
     1501 caagctcgca tttatcccgt tctgcgccag cgcctgacga accttgttga tgtcgtcgag
     1561 cttggcgtag ggccccatcc gcacgcggta ccaggtgccc ttatcgggca ggttcgccgg
     1621 ctcgacgctc gagtcgaagc cgaggatcgc gagccgcgcc ttttggttgt ccgcctcggc
     1681 ggggttctgg aacgaccctg cctggatgaa ataaacgtcc ttgcccgaat cctgttgacc
     1741 gcccttggcg cgcatgcgct cgcggatttc cttctccgtg accggctcct cggacccggg
     1801 caggatgcga tagaagtcga atttcggctt ctccaccgac gcgatcgggg cgggggccgc
     1861 gctttgcggc aatccggtga tcgctggcgg cttgccgctt gccgcggcct tttccggctg
     1921 cttcggcttc gcggtcgtga aggggatcgg cgtgcggttt aagtagaagg cgatgccgag
     1981 cgccaccgca agtccgacca gcactccgac gccgatgccg gcgaggaagt tgcccccctc
     2041 gcgcttgcgc ggcttgcggg tcatcgatcg cgacatcggc atttacatct tctccggggc
     2101 gctgacgcca atgagcgaca gcccgctggc tagggtctgg cgcaccgccg cgcagagcgc
     2161 gagccgcgcg atgcgcagcg cctcgtcgtc gacgaggatg cgctcggcat tatagtagct
     2221 gtgaaactcg gccgcgaggc tgcgcaggta aaaggtgatc gagtgcggcg agagctcgcg
     2281 cgccgcttcg gcgaccagat cggagaattc tccgatccgt cgcgcgagcg cgaattcctt
     2341 ctcattcgtg aggcgtgata ggtcggccgc gctcagttcg gccggatttc ccttccattg
     2401 cccaaacacg ctgcacacgc gggcatgcgc atactgcacg tagtagaccg ggttctcctc
     2461 ggtctgcgcg cgggcgagat cgatgtcgaa cacgaagtcg gcgtcggcgc gccgcgaaag
     2521 caggaagaaa cgcaccgcgt cgcgtcccac ccagtcgagc aggtcgcgca gcgtgacata
     2581 ggaaccggcg cgcttggaga tcttcacctc ttggccgccg cgcatcacgc gcaccatgct
     2641 gtgcatcacg tagtccggat aacccggcgg cacgcctacg ttgagcgcct gcaacccggc
     2701 gcgcacgcgc gccttggttc ccatgtggtc ggtgccctgc acgttgatca ccttggcaaa
     2761 gcctcgccgc cacttggtaa ggtggtaggc cacgtcgggc acgaaatagg tgtaggtatc
     2821 gtccgacttg cggatgacac ggtccttgtc gtccccgtac ttggtgctgc gcagccagag
     2881 cgctccgtcc ttctcgtagg tctggccgct cgcgatcagc gcgttgacca cctcgtcgac
     2941 cttgccttcg gcatagaggc ttgactcgag gtagtagacg tcgaacttga cgccaaactc
     3001 gcgcaggtcg acgtcctgct ctctgcgcag acagtccacg gcgaatctgc gaattggctc
     3061 caaatcctcc aggctgccgc ggtactgctt cgcgatttcc tcgatgtact cgccggcgta
     3121 tccatcggcc ggccagccgg catcgcccgg cttcaaaccg cgagcccgcg cctgcacgga
     3181 gagcgcgaga ttgtggatct gggcgcccgc gtcgttgtag tagaactcac gcgtcacttt
     3241 gtcgccacgc gcttccagaa tcgccgcgag cgcatcgccg atcgcggcct gacggccatg
     3301 gccgacgtgc agcggcccgg tgggattggc cgatacgaac tccacctgca cggaaagcgg
     3361 cctgtccttg gtcgcccgcc cccagtctgc gccggcagcc aaggcggagc ggaccgccgc
     3421 cagctttact tcagggcgga ggcgaaagtt gatgaagccg ggcccggcga tctcgcagcc
     3481 tgcgaagaac ttgtcgaaat ccgggaacgc cgcgagagcc ttgaaagtta gagcttccgc
     3541 gagctgccgc gggttgcgct tgaggcgctt ggcaagctgc agcgcgatcg agcaggcgaa
     3601 gtcgccgtgc tcgggctggc gcggcttctc gaacacgacc tccaactccg gatggtccgg
     3661 agcttctttc gcgagcgctt gccggtacgc ctccgcgagc cgtgcctttg gatcggaagt
     3721 cattgaaaag tcaggaattc tactcgaact cagacagaaa tcggggtcag ag
//
LOCUS       DASTPD010000288         5931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_357109, whole genome shotgun sequence.
ACCESSION   DASTPD010000288 DASTPD010000000
VERSION     DASTPD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357109"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFR57_09560"
     CDS             <1..1023
                     /locus_tag="VFR57_09560"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0259649.1"
                     /translation="TTTPEFGWKGVTDSPLTGITRNPWNKAKTPGGSSGGAAAAVAAG
                     MGPLAVGTDGGGSIRIPCAFTGIFGIKASFGRVPAWPLSPFGTVAHVGPMTRSVTDAA
                     LMLNVLALPDARDWHALPYEPRDWRTGLDHGVADLRIAYSPDLGYAKVDKEVREIVGK
                     AVKVFEDLGAKVEEKNPGFEDPAPLFMTHWFAGAAFLLKGIPKEKHELIDPGLREMAA
                     QGEKVSAHDFLDAQLKRGALGAHMNLFHRDCDLLLTPTLSIPAFDAGKEVADKMLQSR
                     WTDWTPFTFPFNLTQQPAASIACGLTAAGLPVGLHLVGPKYADARVLRAARAFETVRP
                     IRTPNL"
     gene            complement(1020..2144)
                     /locus_tag="VFR57_09565"
     CDS             complement(1020..2144)
                     /locus_tag="VFR57_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259650.1"
                     /translation="MKKVTILLAALVFSLETSAQVNVAATGEAIVAALNKRDLDGVLA
                     MMDVEAVSHIVTKGLGLSDGDRESLRKGFGKALRTNVEIGMRAMEASKGTAKYLRSGV
                     RDSRPYALLRYDLGDQGTDYVEYYLSPSGKVEDWYVHSMATLYSTSARLGLATVLKTD
                     SMLFSLFGIRMASSADAKPFTELRTRLQAQDFAGAYRALDTFPEGFRKTRQWALMRVT
                     FGARIDEATHRAALRYLAANFGAEADLQFMLIDHYFFEEKFDRALASLSALERAVGGE
                     DGATANLRGSILVGAKRFNDAAGACRRGMALEPDHKPAYWCLVSVGIATRDGKTAVEG
                     LKAYEKAFSTEFDLEKLGAIDDYKEIARTPEFAAWKKSRR"
     gene            complement(2170..2604)
                     /locus_tag="VFR57_09570"
     CDS             complement(2170..2604)
                     /locus_tag="VFR57_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259651.1"
                     /translation="MPHSFLFEEGTWRVEGEQRDASGTTAAVSGENSIRHETGRWHFE
                     AVLRLRGDPLRVTHNRYQIEPFLPGAVSTHWSSNNPVLGALRGRFVIAGDAILSAYTS
                     PTLRFRGFECIQQRDARRYTVRGAMLEEDKLVSTWALDLTRI"
     gene            complement(2604..2948)
                     /locus_tag="VFR57_09575"
     CDS             complement(2604..2948)
                     /locus_tag="VFR57_09575"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU0259652.1"
                     /translation="MIAVIFEFTADARRREEYLQLAAMLNEEVKGFDGFLGIERFQSL
                     SAPDRYVSLSFWRDEEAVRKWRNVHKHREAQAKGRGGIFSSYRLRVCSVIRDYGMKER
                     GEAPKDSIAAHD"
     gene            complement(2945..4000)
                     /locus_tag="VFR57_09580"
     CDS             complement(2945..4000)
                     /locus_tag="VFR57_09580"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEU0259653.1"
                     /translation="MRAFVYNGLPARVVFGAGALARLPEELDRLGAKRALVLSTPEQA
                     ASARQVASALGARCAGTYDKAAMHVPVEIAEDARRTARELGADCCVTVGGGSTTGLGK
                     AIALTSAVPILAVPTTYAGSEMTPIYGITEGGAKKTGRDVRVLPKAVIYDPTLTLSLP
                     PALSAASGMNAIAHAVEALYSHEGNPIISLMAEEGIRALALALPAIMKSPSDIGARSD
                     ALYGAWLCGVVLGSAGMALHHKLCHVLGGSFNLPHAETHSIILPHAVRYNHDAAPEAM
                     ARIERAIQAEDAAAGIYDLEKRLGLPMRLADVGMEEGDLERAARIATEAPYPNPRKVD
                     YAAVLQLLRSAYLGHRP"
     gene            complement(4001..4948)
                     /locus_tag="VFR57_09585"
     CDS             complement(4001..4948)
                     /locus_tag="VFR57_09585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259654.1"
                     /translation="MRKLLAALLIAVPCFASAQSYPAKAVRILVPFPPGGTTDLIARI
                     VQPRFQEFMGQTVLIENRSGAGGSVGAAEVARAPADGYTLLMVFDTHAVNHHIFKMAP
                     DPFKQLEHICLMVRSPSALVGVTSFAPNNLREVVAYAKANPEKVTYSTPGSGSSNHLA
                     ALLLEQVAGIKMTHVAYKGGGPMIQALLSQQVHISFFSLPLMVPHIKAGKMKGLAVGS
                     RARVPQLPDVQTLSETFPGFEQYSWFGILGPAGLPKDVAAKVHRETVRTLQAPDVNGK
                     LTEQGFEIVASSPEEFVKFVQGESDKLGKLIRENKIVAE"
     gene            4985..5383
                     /locus_tag="VFR57_09590"
     CDS             4985..5383
                     /locus_tag="VFR57_09590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0259655.1"
                     /translation="MKKQQIRSSKLAEPSGHFSQAIAAPAKGVLVFVSGMTARRPDGK
                     VVGVGDVEEQTRQVCENLKAALEAAGGTLDDVCRVDVYLRNIGDRERVNKVRRQFWRG
                     TPPASTLVEVSKLASPEFLVEISAIALVSP"
     gene            complement(5355..5900)
                     /locus_tag="VFR57_09595"
     CDS             complement(5355..5900)
                     /locus_tag="VFR57_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259656.1"
                     /translation="MTSVPEFSFSHMGIFVTDPARMEDFYTRVLGFAVTDRGMLGSVS
                     LVFLSRDAREHHQIVLAAGRPAAAAFNPINQISFRMADFAGLREMHRRLEEEGVRELS
                     PASHGNALSVYFKDPEGNRIELFVDTPWYVEQPVRVPLDMSLSDAEIWAWAERDAHSR
                     AGFMPVEEWRAGLRAKLARSR"
BASE COUNT          984 a         1978 c         1955 g         1014 t
ORIGIN      
        1 accaccacgc ccgagttcgg ctggaagggc gtgaccgaca gcccgctcac cggcatcacg
       61 cgcaatccct ggaacaaggc gaagactcct ggaggctcct ctggcggcgc ggcggcggcg
      121 gtggccgcgg ggatgggtcc actcgccgtg ggcaccgacg gcggcggctc catacggatc
      181 ccgtgcgcat tcacgggcat cttcggcatc aaggcctcct tcggccgcgt gcccgcgtgg
      241 ccgctatcgc ccttcggcac ggtggcgcac gtcgggccga tgacgcgcag cgtcacagat
      301 gccgcgctga tgctcaacgt actcgcgctg cccgacgcgc gcgactggca cgcgctgccc
      361 tacgagccgc gcgactggcg cacgggtctc gaccacggcg tcgccgacct gcgcatcgcg
      421 tactcaccgg atctgggata tgcgaaggtc gacaaggaag taagagagat agtcggcaaa
      481 gccgtgaagg tgttcgagga tctgggagcg aaggtcgagg agaagaatcc cggtttcgag
      541 gacccggcgc cgcttttcat gacgcactgg ttcgcgggag ccgcgttcct gctcaagggc
      601 atcccgaagg agaagcacga gctgatcgat ccgggcctgc gagagatggc cgcgcagggc
      661 gagaaggtca gcgcgcacga cttcctggac gcgcaattga agcgcggtgc gctcggtgcg
      721 cacatgaatc tcttccaccg tgactgcgac cttctgctga cgccgacgct ttccattccc
      781 gcgttcgacg ccggcaagga ggtggcggac aagatgctgc agagccgctg gaccgactgg
      841 acccctttca ccttcccatt caacctcacc cagcagccgg cggcgtcgat tgcctgcggt
      901 ctcaccgcgg ccgggttgcc ggtcggtctg cacctcgtcg ggccgaagta cgcggacgcg
      961 cgcgtcctgc gcgccgcgcg cgcgttcgag actgtgcggc caataagaac gccaaacctc
     1021 tagcgccggc tcttcttcca ggcggcgaat tccggagtgc gcgcgatttc cttgtagtcg
     1081 tcgatcgcgc caagcttttc gaggtcgaac tccgtgctga acgccttctc gtaggccttg
     1141 aggccctcga ccgcggtctt gccgtcgcga gtggcgatgc caacgctgac caggcaccaa
     1201 taggccggct tatggtcggg ctcgagcgcc atgccgcggc ggcaagcgcc tgcggcgtcg
     1261 ttgaagcgct tcgccccgac caggatgctg ccgcgcaggt tcgccgtcgc accatcctcg
     1321 ccgcccacgg cccgctccag cgcgctgagc gaagcaagcg cacggtcgaa cttctcttcg
     1381 aagaaatagt ggtcgatcag catgaattgc agatcggcct cggcgccgaa gttcgccgcc
     1441 aggtagcgca gcgcggcgcg gtgcgtggcc tcgtcgatgc gcgcgccgaa cgtgacccgc
     1501 atcagtgccc actggcgcgt cttgcggaag ccttcgggga acgtgtcgag cgcgcggtac
     1561 gcgccggcga aatcctgcgc ctgcagccgg gtgcgcagct cggtgaacgg cttggcatcg
     1621 gcgctgctcg ccatacggat gccgaacagg ctgaacagca tcgagtcggt cttgagcacc
     1681 gtggcgagcc cgaggcgtgc ggaggtgctg tagagcgtcg ccatgctgtg cacgtaccag
     1741 tcttcgacct tgcccgaggg cgacaggtag tactcgacgt agtccgtccc ctggtcgccc
     1801 aggtcgtagc gcagcagcgc gtacggcctg gaatcgcgta ccccgctgcg caggtacttc
     1861 gccgttccct tgctcgcctc catggcgcgc atgccgatct cgacgttggt ccgcagcgcc
     1921 ttgccgaagc ccttgcgtag cgactcgcgg tcgccgtcgc tcaagcccag gcccttcgtc
     1981 acgatgtggc tcactgcctc gacgtccatc atggcgagaa cgccgtcgag gtcgcgcttg
     2041 ttgagagcgg cgacgatcgc ttccccggtc gccgcgacat tcacctgcgc cgaagtctcg
     2101 agcgagaaga cgagcgcggc gagcaggatt gtgacttttt tcatctaact cccctaagtc
     2161 tgtgaggagc tagattctcg tcaaatccag cgcccaggta gaaacaagtt tgtcctcttc
     2221 cagcatcgcg ccgcgcaccg tgtagcgccg cgcatcgcgc tgctggatgc actcgaagcc
     2281 gcggaaccgc aaagtggggc tggtgtaggc cgagagaatc gcgtcacctg cgatgacgaa
     2341 gcgtccgcgc agcgcgccga gcaccgggtt gttcgagctc caatgtgtcg agaccgcacc
     2401 gggaaggaag ggctcgatct gatagcggtt gtgcgttacg cggagcgggt cgccgcgcag
     2461 gcgcagcacc gcctcgaagt gccagcggcc ggtctcgtgg cggatcgagt tctcgcccga
     2521 gacggcggcg gtggtgcccg aggcgtcgcg ttgctcgccc tcgacgcgcc aggttccctc
     2581 ctcaaagagg aagctgtgcg gcactagtcg tgcgcggcga tgctgtcctt cggcgcctcg
     2641 ccgcgctcct tcatcccgta atcccggatg acgctgcaga cgcgcagccg ataggagctg
     2701 aagatgccgc ccctgccctt cgcctgcgcc tcccgatgct tgtgcacgtt gcgccatttg
     2761 cgcaccgcct cctcgtcgcg ccagaaggag agcgacacgt agcggtcggg cgcgctcagg
     2821 ctctggaagc gctcgatgcc gaggaagccg tcgaatccct tcacttcttc attgagcatg
     2881 gcagcgagct gaagatattc ctcgcggcgc cgggcgtcgg cggtgaactc gaaaatgacg
     2941 gcgatcatgg acgatgcccc aggtaagcgc tgcgcaggag ctggagtacg gcggcgtaat
     3001 ccactttccg cggattcggg taaggcgcct cggtcgcgat gcgcgccgcg cgctcgaggt
     3061 cgccttcctc catgccgacg tcggcgagcc gcatgggcag cccgagccgt ttctcgaggt
     3121 cgtagattcc ggccgcggca tcctcagcct ggatggccct ttctatgcgc gccatggcct
     3181 ccggcgcagc gtcgtggttg taccgcaccg cgtgcggcag aatgatcgag tgggtttcgg
     3241 cgtgtggcaa gttgaagctg cccccaagca catggcaaag cttgtggtgc agcgccatgc
     3301 cggccgatcc gagcaccacc ccgcacagcc acgccccata gagcgcatcg cttctcgcgc
     3361 cgatatctga gggtgatttc attattgcgg gcaaggcaag cgcgagcgca cgtatgcctt
     3421 cctctgccat gagcgagatg atgggattac cctcgtgcga atagagcgcc tcgaccgcgt
     3481 gcgcgatggc gttcatgccg ctcgccgcgg agagcgcggg cggaaggctc agcgtcagcg
     3541 tcgggtcgta gatgaccgct ttcggcagga cgcgcacgtc gcgcccggtc ttcttggctc
     3601 cgccttcggt gattccatag atcggcgtca tctccgagcc cgcgtaggtg gtcggcacgg
     3661 ccaggatcgg aacggccgag gtgagcgcga ttgccttgcc gagccccgtg gtcgagccgc
     3721 cgccgacggt gacgcagcaa tcggcgccga gctcgcgggc ggtgcggcgc gcgtcctcgg
     3781 cgatctccac cggcacgtgc atcgcggcct tgtcgtaggt cccggcgcac cgggcgccga
     3841 gggcgctcgc cacctgccgc gccgaggctg cctgctcggg agtggagagc acgagcgcgc
     3901 gctttgcacc gagccggtcg agctcttcgg gcaatcgggc cagcgccccg gcgccgaaaa
     3961 cgacccgcgc cggcaggccg ttgtagacga aggccctcac ttattccgca acgatcttgt
     4021 tctcgcggat cagcttgccg agcttgtcgg attcaccctg cacgaacttg acgaactctt
     4081 ccggcgagct cgcgacgatc tcaaaaccct gctcggtgag cttgccgttc acatccggcg
     4141 cctgcagcgt gcgcaccgtc tcgcggtgaa ctttcgctgc gacatcctta ggcagacccg
     4201 ccgggccgag gatcccgaac caggaatatt gctcgaagcc ggggaaggtc tccgacagcg
     4261 tctgcacgtc tgggagctgc ggcacgcgcg cgcggctgcc gaccgcgagg cccttcatct
     4321 tgcccgcctt gatatgcggc accatcagcg gcagcgagaa gaacgagatg tgcacctgct
     4381 ggctgagcag cgcctggatc atcggcccgc cgcccttgta ggcgacgtgc gtcatcttga
     4441 tgccggcaac ctgctcgagg agcagcgccg ccagatggtt ggagctgccc gagcctggag
     4501 tcgagtaggt cactttctcc gggttcgcct tggcataggc caccacctca cgcaggttgt
     4561 tgggcgcgaa gctcgtgacg ccgacgagcg cgctcggcga tctcaccatc aggcagatgt
     4621 gctcgagctg cttgaacggg tcgggcgcca tcttgaagat gtggtggttc accgcatgcg
     4681 tgtcgaacac catgagcaag gtgtagccgt cggcgggcgc cctggccacc tcggccgcgc
     4741 cgaccgagcc gccggcgccg gagcgattct cgatcagcac cgtctggccc atgaactcct
     4801 ggaacctcgg ctgcacgatg cgcgcgatca ggtcggtcgt gccgccgggc gggaacggaa
     4861 cgaggatgcg caccgccttc gccgggtagg attgagcaga cgcaaagcat ggaaccgcaa
     4921 tcagcagcgc ggcgagcagc tttcgcatgg cctcctcctc tcgccttcag tgtaacctag
     4981 ccccatgaag aagcagcaga tccgctcaag caagctcgcc gagccgagcg ggcacttttc
     5041 gcaggcgatc gcggcgccgg ccaagggtgt gctggtgttc gtgtccggga tgacggcgcg
     5101 ccgcccggac ggcaaggtcg ttggcgtcgg cgacgtggag gagcagacac gccaggtgtg
     5161 cgagaacctc aaagctgcgc tcgaggccgc gggcgggacg ctcgacgacg tctgccgggt
     5221 cgacgtctat ctgcgcaaca tcggcgaccg cgagcgcgtc aacaaggtgc gccggcagtt
     5281 ctggcgcggc acaccgcccg cctcgacgct ggtcgaggtg tccaagctcg cctcacccga
     5341 gttcctggtc gagatcagcg cgatcgcgct agtttcgccc tgaggcccgc gcgccactcc
     5401 tcgaccggca tgaaccccgc ccggctgtgg gcatctcgct cggcccacgc ccagatctcg
     5461 gcgtcggaaa gactcatgtc gagcgggacg cgcaccggct gctccacgta ccagggcgtg
     5521 tcgacgaaaa gctcgatgcg gttgccttcc gggtccttga agtagaccga aagcgcgttg
     5581 ccgtgcgacg ccggcgaaag ttctctcacg ccctcctctt ccagcctgcg gtgcatttcg
     5641 cgcagcccgg cgaaatcggc catgcgaaaa gagatctggt tgatggggtt gaatgcggcc
     5701 gcggccggcc tgccggccgc caggactatc tgatggtgct ctctcgcatc gcggctcaag
     5761 aaaaccagcg aaactgaacc aagcatgccc ctgtcggtaa ccgcaaaacc gaggacgcgt
     5821 gtgtaaaaat cctccatccg cgcggggtcg gtcacgaaga tccccatgtg gctgaacgaa
     5881 aactccggaa ccgaggtcat cgcttatgaa gctcgccagt ttcagcactg c
//
LOCUS       DASTPD010000289         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_969850, whole genome shotgun sequence.
ACCESSION   DASTPD010000289 DASTPD010000000
VERSION     DASTPD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969850"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VFR57_09600"
     CDS             <1..1389
                     /locus_tag="VFR57_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901136.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEU0259657.1"
                     /translation="AANVSLAWFEHMKRYWGMEPVQFAFGVMSRSKQITWENLELRDP
                     RFIEEIHRWFAQKVKAQGFDIDPANPPVPMFTPFRLRDMVVENRVVVSPMDQYSAVDG
                     VPGDWHFVHLGSRAVGGAGLVYVEMTCPSPEARISPGDTGLWNETQRDAFKRIVDFCH
                     ANSRAKMCMQLGHSGRKGSTQLGWERMDHPLESGNWPLVSASPIPYYEGISQVPREMT
                     RADMDKVLSDFKKSTLFADQAGFDMLELHMAHGYLLASFISPLTNRRKDEYGGSIENR
                     MRFPLQVLSACRNAWPEGKPISVRISATDWAPGGLTGEDLMALTRMLKEAGCDMIDCS
                     TGQTVPHQKPVYGRMYQAPFADWVRNEVGIATMTVGAVTTPDQVNTLLAAGKADLVAL
                     ARPHLANPYFTLQASAWYQHMDQYWPPQYLSGRDQAFRNAARERAEQMDLRIKARPAS
                     HEVKEGPVKRAA"
     gene            1386..2876
                     /locus_tag="VFR57_09605"
     CDS             1386..2876
                     /locus_tag="VFR57_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0259658.1"
                     /translation="MSETLWGLIERRARDAPHAAAVLFEREAISNRDFHQRALSLARG
                     LAGLGVGKGDVVAVQLPNIPEYLLSYAAICALGATLQPVHMPYRRAELATLLKHSGTR
                     VFLCLSRFKEESPAQEAASVVEQVVTLGEPVDGAKAFGSLTAGARLERVSEGSPEDRF
                     LLLYTSGTTDEPKGVPHAQRGFLANAMASVPEMEIAKHEVILSAAPLTHLYGLYAYHL
                     SLCSGAAMSLLPAFTPPALASIIEKHRVDCVFAGPAHFKPLLDGGLLERHDFSSLRFA
                     CLSGSPVPPELAAAVEKKLPKGKTIQLWGMTELQAGSFSRPRDPADVRHATAGAATPG
                     TELRVAGERLQVRGISLFSGYLKNPKATREVFSADGWFETGDTAELSAAGHLRFTGRV
                     KEIINRGGVKYSPLDVEAIIDRMPGIARSAIVPFPDAVLGERACVFVQPAPGAARPTL
                     EAIIRALDSAGVAKFKWPERLELVAAMPITPTQKVMRGRLRELLQR"
     gene            2873..3556
                     /locus_tag="VFR57_09610"
     CDS             2873..3556
                     /locus_tag="VFR57_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0259659.1"
                     /translation="MISLYELKGKRERRYSLFSWRTRMALKHKGLEFESCPVRMSDKA
                     AIAFSGGKTVPVIKDEDTAVRDSWKIAEYLESRYADAPPLFGGEIGRGVSAAFNTWVD
                     RAVVPAMLPVIAADIHERVDPDDESYFRQTMEKALKTTLEESRARRDAAVLNLGRVLS
                     PMQEACKRQPFISGASAAYADYILFSVFQWARVMSPQELLGPEDPLCRWRERMLELFG
                     GFARNVPIA"
     gene            3553..>3921
                     /locus_tag="VFR57_09615"
     CDS             3553..>3921
                     /locus_tag="VFR57_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259660.1"
                     /translation="MKNLFLATLLLFTLPAQAALDIKDIALGAKEQDVKRAFPSAHCR
                     PLQWESKAADRRCDDSRVAFGEVEVQVTFYLKKDAVEAFDVRFDTRELERFVNFLKSR
                     YGAPVTETRDTFRRKDGGERQ"
BASE COUNT          684 a         1263 c         1289 g          685 t
ORIGIN      
        1 gcggcgaacg tttcgctcgc gtggttcgag cacatgaagc gctactgggg catggagccg
       61 gtgcagttcg ccttcggcgt gatgtcgcgc tccaagcaga tcacctggga gaacctggag
      121 ctgcgcgacc cgcgcttcat cgaggagatc caccgctggt tcgcgcagaa ggtgaaggcg
      181 cagggcttcg acatcgatcc tgcgaacccg ccggtgccga tgtttacgcc ttttcgcctg
      241 cgcgacatgg tggtcgagaa ccgcgtcgtg gtgtcgccga tggaccagta ctccgcggtc
      301 gacggcgtgc ccggcgactg gcatttcgtg catctcggca gccgcgcggt cggcggcgcg
      361 gggctggtct atgtggagat gacctgccct tcgcccgagg cgcgcatctc gcccggagac
      421 accgggcttt ggaacgagac gcaacgggac gccttcaagc gcatcgtgga tttttgccac
      481 gcgaattcca gggcgaagat gtgcatgcag cttgggcatt ccggccgaaa aggctcgact
      541 caactcggtt gggagcgcat ggaccacccg ctcgagagcg gcaactggcc cctggtttcc
      601 gcctcgccga ttccgtacta cgaaggcatc tcgcaggtcc cgcgggagat gactcgcgcg
      661 gacatggaca aggttctgtc ggacttcaag aaaagcactc tgtttgccga tcaggcgggc
      721 ttcgacatgc tcgagctcca catggcgcac gggtatcttc tcgccagctt catttcgccg
      781 cttaccaacc ggcgtaagga cgagtacggc ggaagcatcg aaaaccgcat gcgctttccg
      841 ctccaggtcc tcagcgcgtg ccggaacgca tggcccgagg ggaagccgat ctcggtgcgc
      901 atctctgcga ccgactgggc gccgggcggg ctcaccggcg aagacttgat ggcgctcacg
      961 aggatgctca aggaggcggg ctgcgacatg atcgactgct cgaccggcca gaccgtgccg
     1021 caccagaagc cggtgtacgg gcggatgtac caggcgccgt tcgcagactg ggtgaggaac
     1081 gaagtcggga tcgccacgat gacggtgggc gcagtgacta cacccgatca ggtgaacacg
     1141 ctgcttgctg ccggcaaggc cgacctcgtt gcgctggcgc ggccgcacct cgccaatccg
     1201 tacttcacgc tccaggcctc agcctggtac cagcatatgg accagtactg gccgccccag
     1261 tacctttcgg gccgcgacca ggcgttccgc aacgcagcgc gcgagcgtgc cgagcagatg
     1321 gacctgcgca tcaaggcgcg gccggcgagt catgaagtga aggaaggccc ggtgaagaga
     1381 gccgcgtgag cgagactctt tggggactca ttgagcgccg tgcccgggat gctccgcacg
     1441 ctgcagcagt tctgtttgag cgcgaagcga tctccaaccg agactttcac cagcgggccc
     1501 taagtcttgc ccgcggactc gccgggctgg gcgttggcaa aggagatgtc gtagcggtcc
     1561 agctacccaa catcccggaa tacctgctct cgtacgccgc gatctgcgcg ctcggcgcga
     1621 cgctgcaacc ggttcatatg ccgtaccggc gcgccgagct tgcaaccctg ctcaagcact
     1681 ccggaacccg cgtgttcctg tgcctttcgc gcttcaagga agaatctcca gcccaggaag
     1741 ccgcttcggt ggtggaacag gtcgtcacgc tcggcgagcc ggtcgacgga gcaaaagcct
     1801 tcggatcgct cacggcaggc gcgcggcttg agcgagtgag cgaaggctcg cccgaagatc
     1861 gcttcctgct tctctatacg tcgggcacca cggatgaacc gaaaggcgtg ccgcacgcgc
     1921 agcgcggctt tcttgccaac gcgatggcct cggtaccgga gatggaaata gcgaaacacg
     1981 aggtgattct gtccgcggcg cccctcactc acctctacgg tctgtacgcg tatcatctta
     2041 gcctctgctc cggtgccgcg atgtcgttgt tgcccgcatt cacaccgccc gctcttgcga
     2101 gcattattga aaagcatcgg gtggactgcg tgttcgccgg gccggcgcat ttcaagccgc
     2161 tgctggacgg cgggctgctc gaacggcacg acttctcgtc gctgcgcttc gcctgcctct
     2221 ccggaagccc ggtgccgccg gagcttgccg cggcggtgga gaagaagctg cccaaaggca
     2281 agacgatcca gctttggggc atgacagagc tgcaggcggg ctccttctcg cggccgcgcg
     2341 accccgccga cgtgcgccac gccacggcgg gcgctgccac ccccggaacc gagcttcgcg
     2401 tcgcgggcga gcgactgcag gtgcggggga tttctctttt ttccggctat ctgaaaaacc
     2461 cgaaagccac gcgcgaagtc ttcagcgccg acggctggtt cgagacaggc gacaccgcgg
     2521 agctgtccgc ggccggtcat ctgcgcttca ccgggcgcgt taaggagatt attaaccgcg
     2581 gcggcgtgaa atactcgccg ctcgacgtgg aggcgatcat cgaccgtatg ccgggcattg
     2641 cgcgctcagc catcgtgccc ttcccggacg cggtgctcgg cgagcgcgcc tgcgtgttcg
     2701 tgcaaccggc ccctggcgcc gcgcggccga cgctggaagc gataatccgc gcgctcgata
     2761 gcgccggcgt ggcgaagttc aagtggcccg agcgcctgga gctcgtcgcg gcgatgccga
     2821 tcacgccgac gcagaaggtc atgcggggcc ggctgcgaga gctgctgcag cgatgatttc
     2881 gttgtacgag ctgaagggca agagggaacg acgctacagt ctgttctcct ggcgcacacg
     2941 catggcgctc aagcacaagg gcctggagtt cgagtcgtgc ccggtgcgca tgtcagacaa
     3001 ggcggcgatc gctttctcgg gcggaaagac cgttccggta ataaaggatg aggacaccgc
     3061 agtgcgcgac tcctggaaga tcgcggaata cctggagagc cgctacgccg atgccccgcc
     3121 gcttttcggc ggcgagatcg gccgtggcgt gtcggccgcc ttcaacactt gggtcgaccg
     3181 cgccgtcgtg ccagcgatgt tgccggtgat cgcggccgac atccatgagc gcgtcgaccc
     3241 cgatgacgag tcctattttc gccagacgat ggagaaggcg ctgaagacca cgctggaaga
     3301 gtcgcgcgcg cggcgcgacg cggccgtgct caacctcggc agggtgctct cgcctatgca
     3361 ggaggcgtgc aagcgccagc ctttcatctc cggcgcgagc gccgcgtacg ccgactacat
     3421 tctcttctcg gtgttccagt gggcgcgcgt catgagccca caggagctgc tggggcccga
     3481 ggacccactg tgccgctggc gcgagcgcat gctcgagctc ttcggcggct tcgcccgcaa
     3541 cgtaccgatt gcatgaaaaa tctctttctt gccacactgc tcttgttcac actgccggcg
     3601 caggcagcgc tcgacatcaa ggacatcgcg ctcggcgcca aggagcagga cgtcaagcgc
     3661 gccttcccca gcgcgcattg ccggccgctg caatgggaaa gcaaggctgc cgatcgccgc
     3721 tgcgacgatt cgcgcgtcgc ctttggcgag gttgaggttc aggtcacgtt ctatttgaag
     3781 aaggacgcgg tcgaagcgtt cgatgtgcgc ttcgacacgc gcgagctaga gcgcttcgtc
     3841 aattttctta agtcccgcta cggcgccccc gtcaccgaga cgcgcgacac cttccggcgc
     3901 aaggacgggg gcgagcgcca g
//
LOCUS       DASTPD010000290         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_562436, whole genome shotgun sequence.
ACCESSION   DASTPD010000290 DASTPD010000000
VERSION     DASTPD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562436"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..257)
                     /locus_tag="VFR57_09620"
     CDS             complement(102..257)
                     /locus_tag="VFR57_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259661.1"
                     /translation="MKRFFALLFANGRALLIPGSENTAGHGTTAQAKFWAKERAQLLQ
                     NAPRRAQ"
     gene            complement(273..1082)
                     /locus_tag="VFR57_09625"
     CDS             complement(273..1082)
                     /locus_tag="VFR57_09625"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU0259662.1"
                     /translation="MKAPGEQQYLDLVAEVLEHGTRKSDRTGTGTLSVFGRQLRFGLE
                     DSFPLLTTKKLHLKSILCELLWFLRGDTNVRWLQERGVTIWDEWADENGELGPVYGYQ
                     WRHWRTADGKEVDQIANLIQSLRHKPDSRRHVVSAWNPADIDRMALPPCHLLFQFYVA
                     GGRLSCQMYQRSADLFLGVPFNIASYSALTLMVAQVTGLQPAEFILTLGDAHLYLNHL
                     EQAREQVTRKPRAFPRLRLNAAVRELFEFRYEDFTLENYDPHPAIKAPIAV"
     gene            complement(1079..1873)
                     /locus_tag="VFR57_09630"
     CDS             complement(1079..1873)
                     /locus_tag="VFR57_09630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0259663.1"
                     /translation="MTSEQQAVPGYTVVHIIGQGGQAQVFLAEREHDGLRVALKVLNR
                     KLRNDPVFLERFVREYKLVASLNSNYVARIYDQGFSGDFPYIAMEFLPSGTLAARIRE
                     GLTARAALRIASQIAQALDTIHSHGIVHRDLKPANILFRADGRPVIVDFGLARDMRIN
                     STLTVAGQFLATPRYMSPEQCLGNSVDARSDLYSLGAMLYEMMTGNKIYDHAHSADVI
                     AMHVHAPVPQLTGMLSVHQPLLERLLAKNPEDRFQSAAEVIAEIGL"
     gene            complement(1890..2540)
                     /locus_tag="VFR57_09635"
     CDS             complement(1890..2540)
                     /locus_tag="VFR57_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0259664.1"
                     /translation="MQAVEIETGSNPVGAVIWLHGLGADGHDFEPVVPELRLKRAVRY
                     IFPHAPIRPVTINMGMRMRAWYDIFQFGGGPEDGAGIRASQKILEELIGQETRRGLTP
                     RQIVLAGFSQGGAIALHTGLRFAERLAGILALSTYLPLASTVEEEGSQVNRDVPIFMA
                     HGEFDDIIPLRRAEGSRALLAKLGYKVEWRAYPMPHSVCAPEIGDIAKFLAQVLED"
     gene            complement(2550..3080)
                     /locus_tag="VFR57_09640"
     CDS             complement(2550..3080)
                     /locus_tag="VFR57_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0259665.1"
                     /translation="MVARALLTFALALAGCAGAFQAAPEVRYEPTPMDVVEIMLELAQ
                     VRGDDVVADLGCGDGRLVIEAARRHGARGLCVDIDPKRIAEARRNAAAAGVAERVTFL
                     NQDLFGTELRGVSVVMLFLSPRFNLQLRPKLERELASGARVVSHWHDMGDWPPSRTVR
                     VTSDGRERAVYLWTMP"
     gene            complement(3109..3660)
                     /gene="mog"
                     /locus_tag="VFR57_09645"
     CDS             complement(3109..3660)
                     /gene="mog"
                     /locus_tag="VFR57_09645"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEU0259666.1"
                     /translation="MKEDLLIGLVSISDRASAGVYKDEGIPSLKEWLASAIASPAWQD
                     ETRLIADEQPLIEATLKELVDDKGCSLVLTTGGTGPAPRDVTPEATLAVADKVMPGFG
                     EHMRAVSLKYVPTAILSRQIAVVRKHALIINLPGQPKAIRETLDGVFAAVPYCVDLIG
                     GPYIETNEAVVKAFRPKSALRKK"
BASE COUNT          657 a         1221 c         1304 g          640 t
ORIGIN      
        1 ggagaggaac acataggggt agacgaaaac gccgagcgcg aagccaccga acgcgccgag
       61 caccagcgct gcgaaaaacg tgatgacgta agcgcgcacg gctattgcgc gcggcgcggt
      121 gcgttctgca gaagctgtgc acgctctttc gcccagaact tggcctgcgc cgtggtgccg
      181 tgcccagccg tgttctcgct gcccggaatc agcagcgcgc gtccgttggc gaaaagcagg
      241 gcgaagaagc gcttcatggg tcctcctcgg ggtcagaccg cgatgggtgc cttgatggcg
      301 ggatgagggt cgtaattctc cagcgtgaag tcttcgtagc ggaactcgaa aagctcgcgc
      361 accgctgcat tcagccgcag ccgggggaaa gcgcgcggct tgcgcgtgac ttgctcgcgc
      421 gcctgctcca gatggttgag gtacaggtgc gcgtcgccga gcgtgaggat gaactcggcg
      481 ggctgcagcc cggtcacctg cgccaccatc agcgtgagcg cggagtagga cgcgatgttg
      541 aacggcacgc cgaggaaaag gtccgcgctg cgctggtaca tctggcatga gagcctgccg
      601 cctgcgacat agaactgaaa gagcaggtgg caaggcggta gcgccatgcg gtcaatgtcg
      661 gcgggattcc acgcgctgac gacgtgccgg cgcgaatccg gcttgtggcg gagggactgg
      721 atgagattcg ctatctggtc gacttccttg ccgtccgctg tgcgccagtg acgccactga
      781 tagccgtaca ccggcccgag ctcgccgttc tcgtcggccc attcatccca gatggttacg
      841 ccgcgttcct gcagccagcg cacgttggtg tcgccgcgca ggaaccacag cagctcacag
      901 aggatggact tgagatgcag cttcttcgtg gtgagcaggg ggaagctgtc ctcgagacca
      961 aagcgaagct ggcgcccgaa caccgacagc gtccccgtgc cagtgcgatc cgacttgcgc
     1021 gtgccgtgct cgagcacctc cgcgacgaga tcgaggtact gctgctcgcc cggggccttc
     1081 acaggccgat ctcggcgatc acctcggccg cgctctgaaa gcggtcctcg gggttcttgg
     1141 cgagcaggcg ctcgagcagc ggctggtgca ccgaaagcat gccggtcagt tggggcacgg
     1201 gggcgtgcac gtgcatggcg atcacgtcgg ccgaatgggc gtggtcgtag atcttgttgc
     1261 cggtcatcat ctcatacagc atcgcgccca ggctgtagag atcggagcgc gcgtccaccg
     1321 agttgcccag gcattgctcg gggctcatgt accgcggcgt ggcgaggaac tggccggcca
     1381 cggtgagcgt cgagttgatc cgcatgtcgc gcgccagccc gaaatccacg atcactgggc
     1441 ggccgtcggc gcgaaacagg atgttggcgg gtttgaggtc gcggtgcacg atcccgtgcg
     1501 aatggatggt gtcgagcgcc tgcgcgatct ggctggcaat gcggagcgcc gcgcgcgccg
     1561 tcagcccttc acggatgcgc gcggcgagcg tgccggaggg caggaactcc atcgctatgt
     1621 aggggaagtc gccgctgaag ccctggtcgt agatgcgcgc gacatagttg ctgttcaggc
     1681 tggccaccag cttgtattcg cgcacgaaac gctcgaggaa caccggatca ttgcgcagct
     1741 tccggttcag taccttcaga gcgacgcgca gcccgtcgtg ctcgcgctcg gcaaggaaca
     1801 cctgcgcctg cccgccctgg ccgatgatgt ggacgacggt gtagcccggg acggcctgct
     1861 gctcggacgt catccgggca ttttatcttt tagtcctcca gtacctgcgc caagaacttg
     1921 gcaatatcgc cgatctcggg cgcgcagacc gagtgcggca tcgggtaggc acgccactcg
     1981 accttgtagc cgagcttggc cagaagcgcg cgcgacccct cggctcgacg caggggaatg
     2041 atgtcgtcga actcgccatg cgccatgaag atcgggacgt cgcggttcac ctgcgaaccc
     2101 tcctcttcaa cggtggatgc aagcggcaaa tacgtcgaga gcgccagtat cccggcgagg
     2161 cgctcggcga agcgcagccc cgtgtgcagc gcgatcgccc cgccctggga aaatcctgca
     2221 agcacgatct gccgaggggt caaaccccgg cgcgtctcct ggccgatcag ctcctcgagg
     2281 attttttgcg aagcccggat gccggccccg tcctcggggc cgccgccgaa ctggaagatg
     2341 tcgtaccagg cacgcatgcg catgcccata ttgatcgtca cggggcgaat cggcgcatgc
     2401 gggaagatgt atcggacggc gcgcttcaag cgcagctccg gcacgacggg ctcgaagtcg
     2461 tggccgtcgg cgccgaggcc gtgcagccag atcaccgccc cgaccgggtt cgatccggtc
     2521 tcgatctcga cggcttgcat gcggcaagtc taaggcatgg tccagaggta gacggcacgc
     2581 tcgcgtccgt cgctcgtgac gcgcacggtt cggctcggcg gccagtcgcc catgtcgtgc
     2641 cagtgcgaga ccacccgggc gccggaggcg agctcgcgct cgagcttggg gcgaagctgc
     2701 agattgaagc gcggcgagag gaatagcatc accacgctga cgccgcgcag ctcggttcca
     2761 aagaggtcct gattgaggaa ggtgacgcgc tcggcgaccc ccgcggcggc agcgttgcgc
     2821 cgcgcctcgg cgatgcgctt gggatcgatg tcgacgcaca agccgcgcgc gccgtgccgg
     2881 cgcgcggcct cgatcacgag ccggccgtcg ccgcagccga ggtcggcgac cacgtcgtcg
     2941 ccgcgcacct gggcaagctc aagcatgatc tccaccacgt ccatcggcgt gggctcgtag
     3001 cgcacctccg gcgcagcctg aaaggcaccg gcgcagccgg cgagcgccag ggcaaaggtg
     3061 aggagggcgc gagcgaccat tgcgcggcag tatctgaagg actaatgctt acttcttccg
     3121 cagggccgat ttgggacgga aggccttgac caccgcctcg ttggtctcga tgtacgggcc
     3181 gccgatgaga tcgacgcagt agggcaccgc ggcgaacacg ccgtcgagcg tctcgcggat
     3241 cgcctttggc tgcccgggaa ggttgatgat cagagcgtgt ttccggacca ccgctatctg
     3301 gcgcgacagg atcgcggtcg gtacgtactt gaggctcacc gcgcgcatgt gctcgccgaa
     3361 gcccggcatc accttgtccg ccaccgcgag cgtcgcctcg ggcgtcacgt cgcgcggcgc
     3421 gggtccggtg ccgccggtcg tcagcacgag gctgcacccc ttgtcgtcga cgagttcctt
     3481 cagcgtcgcc tcgatgagcg gctgctcgtc ggcgatgagg cgcgtttcat cttgccaggc
     3541 gggggaggcg atcgccgagg cgagccactc cttgagcgac gggatgcctt cgtccttata
     3601 gacgccggcg gaggccctat cgctaatcga gaccagtccg atcagcagat cttctttcat
     3661 ctcacctcga agacttcgag ctccaccgcg tccgtgtggc gcgcacccgt ggcgacgcaa
     3721 acgatacggt tgagccaggc atagcgcgca tccccggtct cgaatcgcgg ggtggttcgc
     3781 atgtagtaca acgaaggatc cacggcttcg ccggcggcga gc
//
LOCUS       DASTPD010000291         7940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_562771, whole genome shotgun sequence.
ACCESSION   DASTPD010000291 DASTPD010000000
VERSION     DASTPD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562771"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VFR57_09650"
     CDS             <1..1561
                     /locus_tag="VFR57_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0259667.1"
                     /translation="GEDVVVTWAAKQLNRPVKWTAERSESYVSDAHGRDHLTSAELAL
                     DKDGKFLAMRVRTTANLGASLSTFASCIPTILYATLLAGQYTTPHIYCEVTAVFTNTT
                     PVDAYRGAGRPEATYVVERLVETAAREMKMSPVEIRKQNFIREFPYQTPVALCYDTGN
                     YQATLDAALKLADVQNFSKRKEESAKHGKLRGIGYACYIEACGIAPSNVAGSLGARAG
                     LFEAGEIRVHPTGTVTVFTGSHSHGQGHETSFAQVVADRLGLPIEAVEVVHGDTSKIL
                     FGMGTYGSRSIAVGGTAIVKALDKIVAKGKKIAAHLLEASESDIEYDRGQFKVAGTDR
                     KKTFGEVAFAAYVPHNYPLDKLEPGLNENAFYDPTNFTFPAGSYVCEVEVDKETGATE
                     IVGFTAVDDFGKVINPMIVEGQVHGGLAQGIGQALTEGCRYEDSGQLVTGSFMDYCIP
                     RSADIPLFNIDTRETPCTHNPLGVKGCGEAGAIGAPAAVMNAITDALGVKDVPMPATA
                     QTVWRSMKKAA"
     gene            1575..2372
                     /locus_tag="VFR57_09655"
     CDS             1575..2372
                     /locus_tag="VFR57_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0259668.1"
                     /translation="MHAFEYHRPSSTSDALSLGKKEEAKYLAGGQSLVQAMKLRLSSP
                     TDLIDLGSLKELSFAKATGSGVEIGAMTRHADVAGSKDVQKTIPALAALAGIIGDRQV
                     RHMGTLGGSLANSDPAADYPAAVLGLGATITTNKRKIDADKFFKSLYETALEPGELIT
                     SVAFPAPKRAAYMKFKNPASRFAIVGVFVADFGAGKVRVAVTGAGPCAFRQADMEKAL
                     AAKFAPESVANIKVKPDGLNNDLHASPEYRAHLITIMAKRAVEAALK"
     gene            complement(2430..3020)
                     /locus_tag="VFR57_09660"
     CDS             complement(2430..3020)
                     /locus_tag="VFR57_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259669.1"
                     /translation="MEMQMVKELMLQSLEHEMGGTKVYETALKCAVNEDLKEEWEGYR
                     QETEKHVQVLHDACLQLEIDPEEQTPGRKITHDMGASLVAAMEAALGAGDKEQAQCVA
                     AECVVLAETVDHFNWQLIGEVAEKMDGAQGKALKEAYQEVEDEEDEHLYHSKGWLREL
                     SLQGLGLKAQLPPPEEKKKVKSASAQARVEQQARKR"
     gene            complement(3085..4068)
                     /locus_tag="VFR57_09665"
     CDS             complement(3085..4068)
                     /locus_tag="VFR57_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259670.1"
                     /translation="MDRRAFVETAGALLLAASGAPRAQPKVARVGFLVALAEGDPENQ
                     ERMAAFQRGLRELGWTEGRNLRTEYRYGAGDAAKIRQQAAEIVGMGPDLIVVQSNLAF
                     AVLRQVDRRIPTVFVAVSDPVGEGFVASLSRPGGNATGFTNYEPEMGGKWLQLLKELA
                     PAVQRALVLLNAKIAANVQLARAAESAAEARGMRVSLAPATDPAEMGNAIAGFARERN
                     GGLIVMPNPSNRVHEKLIIDTCAKNRLPAVYPFTYAAKSGGLAGYGLDSVEMHRLAAS
                     YVDRILKGASPAEMPVQLPNRLELAINLRTAKALGLKVSPALLARANEVIE"
     gene            4175..5086
                     /locus_tag="VFR57_09670"
     CDS             4175..5086
                     /locus_tag="VFR57_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0259671.1"
                     /translation="MQKRFPKSIDETQALLASGEYVADRSLATSLFLTLAMKRPLFLE
                     GEAGVGKTEIGKVVAQALGRELIRLQCYEGLDIAQAAYEWNYPRQMIEIRMAEAEGVK
                     SREGLEQDIFSERFLIRRPLLRALQPLGDPPVLLIDELDRTDEPFEAYLLEVLSDWQI
                     TIPEIGTVRAEEPPIVVITSNRTREIHDAVKRRCFYHWVDYPDAKRELEILRRKAPKA
                     VEGLSREVVAFVQQLRKVDLFKLPGVAETIDWANCLAALDRVVLDPETVNNTLGVLLK
                     YRDDLERVAGEEAERLVGEAKAAAAAA"
     gene            5096..6475
                     /locus_tag="VFR57_09675"
     CDS             5096..6475
                     /locus_tag="VFR57_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEU0259672.1"
                     /translation="MPISVTSLQHYVERWRDQAASAWDSLRRRIGGKPDSTERPAQRL
                     SALSGALLAESGEYASSALAREALSAYQALDAQGRCEFFDALARDHSPAPEAVASAAR
                     AYQTDPSADNLIRLQQSVEAPRQELFRRLNMAPGGTATLVEMRGWLLKNLKENPQWRI
                     IDSDLMHLLRSWFNRGFLRLERIDWRTSAAVLEKLIRYEAVHAIDGWRDLHRRLEADR
                     RCFAFFHPQLPDEPIIFIEVALTEGMAARVQPLLDVETPVASPVDCDCAMFYSITNCH
                     EGLRGISFGNFLIKQVAEELRREFPQLRRFATLSPIPGFRRWLDRRRSERLKELTPLE
                     QLEQPDWHVRDSSDVLQRLLMRLCAYYLLHAKQAEGGHAEPVDPVARFHLGNGAALER
                     LNWMGDTSQAGMARSAGLMVNYVYRLNEVERNHERYWREKLIAASAQVEKLARECLFA
                     APAKGGLAA"
     gene            6478..7659
                     /locus_tag="VFR57_09680"
     CDS             6478..7659
                     /locus_tag="VFR57_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0259673.1"
                     /translation="MQGRLPENVMHFARLLRAAGMRVGSDRVIDCVRALEMAGAQQFP
                     LRRDDWYWTMSAVLLSRQEQRPIFDQAFRIFWRDPKLAERMMSLLLPHAHGRAPKPEQ
                     QQSQRLTDALFSQKQESREGEKLELEARLTFSSREVLSRMDFDTMSAAELAEAKKMLA
                     QLRLPLPLIRTRRRRISPKGKAIDLRATLRESLREGGEVIALVRAAPRTLHPPLVVLC
                     DISGSMNPYSRMFLHFLHAITNDRDRVSVFVFGTRLTNITRQLRHRDVDVAMSRVAEA
                     IKDWSGGTRIGASLREFNFKWGRRVLAQNACVLLVSDGLDREAGEGLAEEMERLAKSC
                     RQLVWLNPLLRYEKFEARPAGVRAMLPHVDLFLPVHNLKSLVDLARTLSTPIKERPLW
                     K"
     gene            7656..>7940
                     /locus_tag="VFR57_09685"
     CDS             7656..>7940
                     /locus_tag="VFR57_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0259674.1"
                     /translation="MTGEQRIAASQQDTWKALNDPEVLKACVPGCESIARVNDNEYQV
                     QMTARVGPVSAKFRGRLSIFDVNAPNSYSLAFEGQGGAAGFAKGAAQVKLS"
BASE COUNT         1361 a         2676 c         2589 g         1314 t
ORIGIN      
        1 cggcgaagac gtggtggtca cctgggcggc gaaacaactg aaccgcccgg tgaagtggac
       61 cgccgagcgc tcggagtcct acgtctccga cgcgcacggg cgcgaccacc tcacgagcgc
      121 cgagctcgcg ctcgacaagg acggcaagtt cctcgcgatg cgcgtgcgca cgaccgcgaa
      181 cctcggcgcg tctctgtcga ccttcgcctc gtgcatcccg accatcctgt acgcgacctt
      241 gcttgccggg cagtacacga cgccgcacat ctactgcgag gtgaccgcgg tgttcaccaa
      301 caccacgccc gtcgacgcct atcgcggcgc cggccgcccg gaagccacct acgtggtcga
      361 gcgcctggtc gagactgcgg cgcgcgagat gaagatgagc ccggtcgaga tccgaaagca
      421 aaacttcatc cgcgagttcc cgtaccagac gccggtcgcg ctctgctacg acaccgggaa
      481 ctaccaggcc accctggatg cagcgctcaa gctcgcggac gttcagaact tcagcaagag
      541 aaaagaagag tcggcaaagc acggcaagct gcgcggcatc ggctatgcct gttacatcga
      601 agcctgcggc atcgcgccct ccaacgtcgc cggctccctc ggcgcgcggg cgggcctgtt
      661 cgaggcgggc gagatccgcg tgcatccgac aggcaccgtc accgtgttca ccggctcgca
      721 ttcgcatggc cagggccacg agacgagctt tgcgcaggtg gtcgccgacc ggctcgggct
      781 gccgatcgag gcggtcgagg tggtgcacgg cgacacttcc aagatcctct tcggcatggg
      841 cacctacggc tcgcgctcca tcgccgtggg tggcaccgcg atcgtcaagg cgctcgacaa
      901 gatcgtcgcc aagggcaaga agatcgctgc gcatctgctg gaagcctccg agtcggacat
      961 cgagtacgac cgcggccagt tcaaggtcgc ggggaccgat cgcaagaaga ccttcggcga
     1021 agtggcgttc gcggcttacg tgccgcacaa ctacccgctc gacaagctcg agcccgggct
     1081 gaacgaaaac gcgttctatg acccgaccaa cttcaccttc ccggcgggca gctacgtgtg
     1141 cgaggttgag gtagacaagg aaaccggcgc gacggaaatc gtcggcttca ccgcggtgga
     1201 cgacttcggc aaggtcatca acccgatgat cgtcgaaggc caggtgcacg gcgggcttgc
     1261 gcagggcatc gggcaggcgc tcaccgaggg ctgccgctac gaagactccg gccagctcgt
     1321 caccggctcg ttcatggatt actgcatccc gcgctccgcc gacataccgc tcttcaacat
     1381 cgacacccgc gagacgccgt gcacgcacaa ccctcttggc gtgaaaggct gtggcgaggc
     1441 gggcgcgatc ggcgcgccgg cggcggtgat gaacgccatc accgacgcgc tcggcgtgaa
     1501 ggacgtgccg atgccggcga cggcgcagac ggtctggcgg tcaatgaaga aagcggccta
     1561 ggaggagatc tttcatgcat gcattcgaat atcaccgtcc atccagcaca agcgacgcgc
     1621 tgagtctcgg caagaaggaa gaagcgaagt acctcgcggg cggacagagc ctggtgcagg
     1681 cgatgaagct gcggctctcc tcgcccacgg acctgataga tctgggctct ttgaaagaac
     1741 tttcttttgc gaaagcaacg ggaagtggtg tcgaaatcgg cgccatgacg cgccatgccg
     1801 acgtcgccgg ctcgaaagac gtgcagaaaa cgatccctgc gctcgccgcg ctcgcaggca
     1861 tcatcggcga ccgccaggtg cgccacatgg gcacgctcgg cggctcgctc gcgaatagcg
     1921 acccggcggc cgactacccg gcggcagtgc tcggcctggg cgctacgatc accacgaaca
     1981 agcgcaagat cgacgcggac aagttcttca agagcctgta cgagaccgcg ctcgagccgg
     2041 gcgagctcat cacctcggtc gcgttcccgg cgccgaaacg cgcggcctac atgaagttca
     2101 aaaacccggc ttcgcgcttc gcaatcgtcg gcgtcttcgt cgccgacttc ggcgccggca
     2161 aggtccgcgt cgcggtcacg ggtgccggcc cctgtgcgtt ccgccaggcc gacatggaaa
     2221 aggcgctcgc cgcgaagttc gcccccgaat cggtggccaa catcaaggtc aagcccgacg
     2281 gcctcaacaa tgacctgcac gcgtcgcccg agtaccgcgc gcacctcatc acgatcatgg
     2341 cgaagcgcgc cgtggaggcc gcgctcaagt aagaaaatcg gggtctgacc ccaagaatcg
     2401 gggtcagacc cgattcttgg ccccggcgtt cagcgcttgc gcgcctgctg ctcgacgcgc
     2461 gcctgtgcgc tggcggactt cactttcttc ttctcctcgg gcggcggcaa ctgcgccttc
     2521 aggccgaggc cctggagcga gagctcgcgc agccagccct tggagtggta gaggtgctcg
     2581 tcctcctcgt cctcgacttc ctggtaggcc tccttcagcg ccttgccttg cgcgccgtcc
     2641 atcttctcgg ctacctcgcc gatcagctgc cagttgaagt gatccaccgt ttcggcgagc
     2701 accacgcact ccgccgccac gcactgcgcc tgctccttgt cccccgcgcc gagcgccgct
     2761 tccatcgccg ccacgagcga ggcgcccatg tcgtgcgtga tcttgcgccc tggcgtctgc
     2821 tcctcggggt cgatctcgag ctgcaggcag gcgtcatgta aaacctgcac gtgcttctcg
     2881 gtttcttggc ggtagccctc ccactcctct ttcaggtctt cgttgacggc gcacttcagc
     2941 gccgtctcgt acaccttggt gccgcccatc tcgtgctcga gcgactggag catcagctcc
     3001 ttgaccatct gcatttccat ggacttctcc tgttcgttgg tggcgaatca ggagagcgcc
     3061 aagcaatggc cgttcccgat ggcgctattc gatcacctcg tttgcccgcg cgagcagcgc
     3121 gggagaaacc ttcaggccca gagccttcgc ggtcctgagg ttgatggcga gctcgaggcg
     3181 attcggcagc tggaccggca tctctgccgg cgaagcgccc ttcaggatgc ggtcgacata
     3241 ggaggccgcc agccggtgca tctcgaccga gtccaggccg tagccggcga ggccgccgga
     3301 tttcgcggcg taggtgaatg ggtagaccgc gggcaggcga ttcttggcgc aggtgtcgat
     3361 gatcagcttc tcgtgcaccc ggttactcgg gttcggcatc acgatcaggc cgccgttgcg
     3421 ctcgcgcgcg aacccggcga tcgcgttccc catctcggcc ggatcggtgg ccggagcgag
     3481 gctcacgcgc atgccgcgtg cctcggcggc ggattccgcg gcgcgcgcga gctgcacgtt
     3541 cgccgcaatc ttcgcgttga gcagcacgag cgcccgctgc accgcgggcg cgagctcctt
     3601 cagcagctgc agccacttgc cgcccatctc cggctcgtag ttggtgaacc cggtggcatt
     3661 gccgcccggc cgcgataggc tcgccacgaa tccctcgccg accgggtcgg agaccgcgac
     3721 gaaaacggtg ggaatgcggc ggtcgacctg ccgcagcacg gcgaacgcca ggttgctctg
     3781 gaccacgatc aggtcgggac ccatgccgac gatttcggcc gcttgctgcc gtatcttcgc
     3841 cgcatcgccc gcgccatagc gatactcggt gcggaggttg cggccctcgg tccagccaag
     3901 ctcccgcagt cctctctgga acgccgccat gcgctcctga ttctccgggt cgccttcggc
     3961 caatgcgacc aggaagccaa cgcgcgcgac cttgggctgc gcgcgcgggg cgccggacgc
     4021 cgcgagcagc agcgcgcccg cggtttcgac gaaggctctc cgatccacgt cgctagtctg
     4081 cccttaggcg aaggcgccgt gttgtcgcag attcacccga acgaaacgga aggaagtggc
     4141 ggtgcatcct caaatgccgg aaaatgaacg tgttatgcag aagcgcttcc cgaaatcgat
     4201 cgacgagacc caggccctgc tcgcctccgg cgagtacgtg gccgaccgaa gcctcgcgac
     4261 ctcgcttttc ctcacgctcg cgatgaagcg cccgctcttc ctcgagggtg aggcgggggt
     4321 ggggaagacc gagatcggca aggtggtggc gcaggcgctc gggcgagaac tgattcggct
     4381 gcagtgctat gagggcctcg acatcgcgca ggcggcttac gagtggaact acccccggca
     4441 gatgatcgag atccgcatgg ccgaggcgga gggcgtgaag tcgcgcgagg gactcgagca
     4501 ggacattttt tccgagcgtt ttctcatccg ccggccgctc ttgcgcgcgc tgcagccgct
     4561 cggcgacccg ccggtactct tgatcgacga gcttgaccgc accgacgagc cgttcgaggc
     4621 gtacctgctg gaagtgcttt ccgactggca gatcacaatc cccgagatcg gtacggtgcg
     4681 cgcggaggag ccgccgatcg tcgtgatcac ttccaaccgc acacgcgaaa tccacgacgc
     4741 ggtgaagcgc cgctgcttct accactgggt cgactacccc gacgcgaaac gcgagctcga
     4801 gatcctgcgc cgcaaggcac cgaaggcggt cgaggggctg tcgcgcgagg tggtcgcgtt
     4861 tgtgcagcag cttcgaaagg tggatctctt caagctgccc ggcgtcgccg agaccatcga
     4921 ctgggcgaac tgcctcgctg cgctcgaccg cgtggtgctc gaccccgaga cggtgaacaa
     4981 caccctgggc gtgctgctca agtaccgcga cgatctggag cgcgtcgccg gcgaggaggc
     5041 cgagcgcctg gtgggcgagg cgaaggccgc ggccgccgcc gcgtgattac cctcggtgcc
     5101 gatatccgta acttccctcc agcactacgt cgagcggtgg cgcgatcagg cggcgagcgc
     5161 ctgggactcc ctgcgccggc ggatcggcgg caagccggat agcactgaac gccccgcgca
     5221 gcgtctaagc gcgctgtcgg gcgcgctgct tgccgagagc ggcgaatacg cgagctcggc
     5281 gctcgcgcgc gaagcgctct cggcgtacca ggcgctcgac gcgcagggcc ggtgcgaatt
     5341 tttcgacgcg ctcgctcgcg accattcgcc cgctcccgaa gccgtggcga gcgccgcgag
     5401 ggcttatcag accgatccat ccgcagacaa cctgatccgg ctgcagcaat cggtggaggc
     5461 gccgcgccag gagctgttcc ggcgccttaa catggccccc ggcggaaccg ccacgctggt
     5521 cgagatgcga ggctggctgc tgaagaacct caaggaaaat ccccagtggc ggatcatcga
     5581 ctctgacctc atgcatctgc tgcgctcgtg gttcaaccgc ggcttcctgc ggctcgagcg
     5641 catcgactgg cgcacttcgg cggccgttct ggaaaagctc atcaggtacg aggcggtgca
     5701 cgcgatcgac ggctggcggg atctccaccg ccgcctcgag gccgaccggc gctgcttcgc
     5761 gttcttccat ccgcagcttc ccgacgagcc gatcatcttc atcgaggttg cgctcaccga
     5821 aggcatggcg gcacgcgttc aaccattgct ggacgtcgaa accccggtcg cgagtccggt
     5881 agactgcgat tgcgcgatgt tctactcgat caccaactgc cacgaggggc tgcgcggcat
     5941 ctcgttcggc aactttctca tcaagcaggt ggcggaggag ctcaggcggg agtttccgca
     6001 gttgcggcgc ttcgctacgc tttcgcccat tcccggcttc cggcgctggc ttgaccgcag
     6061 gcgcagcgag cgcctcaagg agctgacgcc gctcgagcag ctcgagcagc cggactggca
     6121 cgtccgcgac tcctccgatg tcttgcagcg gctattaatg cggctttgcg cgtactacct
     6181 gctgcacgcc aagcaggcag agggaggcca cgcggagcct gtcgacccgg tggcgcgctt
     6241 ccacctcggc aacggggccg cgctcgagcg cctgaactgg atgggcgaca cctcgcaggc
     6301 cggcatggcg cgctcagccg gcctgatggt gaattacgtg taccggctga acgaggtcga
     6361 gcgcaaccat gagcgctatt ggcgtgagaa gctgatcgcc gcctcggcgc aggtcgagaa
     6421 gctcgcgcgg gaatgccttt tcgccgcgcc ggccaaagga ggactggcgg cttgagcgtg
     6481 caaggccgcc tgcccgaaaa cgtcatgcac ttcgcgcgcc tgctgcgtgc ggcgggcatg
     6541 cgggtgggct cagaccgcgt catcgactgc gtacgcgcgc tcgagatggc cggcgcgcaa
     6601 caatttcccc tgcggcgcga cgactggtac tggaccatgt cggccgtgct gctctcgcgc
     6661 caggagcagc gcccgatttt cgaccaggcg ttccgtatct tctggcgcga cccgaagctc
     6721 gccgagcgca tgatgagcct gctcctgccg cacgcgcacg gccgcgcacc caagccggag
     6781 cagcagcaga gtcagcgcct cacggatgcc ctgttttctc aaaagcagga atcacgggaa
     6841 ggcgagaagc tcgagctcga agcgcgcctg accttctcct cgcgcgaggt gctctcgcgc
     6901 atggacttcg acaccatgtc ggcggccgag ctcgccgagg cgaagaagat gctggcgcag
     6961 ttgcggctgc ctttgcctct gatcagaacc cggagaagaa gaatctctcc gaagggaaaa
     7021 gctatcgatc ttcgtgcgac tttgcgggag agcctgcgcg aaggcggcga ggtgattgcg
     7081 ctggtgcgcg cggcgccgag aacgctgcac ccgccgctcg tcgtgctgtg cgacatctcg
     7141 ggctcgatga atccgtactc gcgcatgttc ctgcatttcc tgcacgccat caccaacgac
     7201 cgcgatcggg tctcggtgtt cgtgttcggc acgcgcctca ccaacatcac gcgccagctg
     7261 cgccaccgcg acgtcgatgt cgcgatgagc agggtggccg aagcgatcaa ggactggtcg
     7321 ggcggcacgc gcatcggcgc gagcctgcgc gagttcaact tcaagtgggg ccgacgggtg
     7381 ctcgcgcaga acgcctgcgt gctgctcgtc tccgacggcc tggaccgcga ggcgggcgag
     7441 ggcctcgcgg aggagatgga gcgcctcgcc aagtcctgcc ggcagctcgt gtggctgaac
     7501 ccgctcctgc gctacgagaa gttcgaggcc cggccggccg gcgtgcgcgc catgctgccg
     7561 cacgtggatc ttttcctgcc ggtgcataat ctcaagagcc tcgtcgatct cgcgagaaca
     7621 ctgagcacgc ccataaagga gcgacccctg tggaaatgac cggcgagcag cggatcgcgg
     7681 cctcgcagca ggacacgtgg aaggcgttga acgaccccga ggtgctcaag gcctgcgtcc
     7741 cgggctgcga gtcgatcgcc cgggtgaatg acaacgagta ccaggtgcag atgaccgccc
     7801 gggtcggccc ggtgagcgcg aagttccgcg gccgcctgtc gatcttcgac gtgaacgcac
     7861 cgaactcgta ctcgcttgcc ttcgagggcc agggcggcgc cgccggcttt gccaaggggg
     7921 cggcgcaggt caagctctcc
//
LOCUS       DASTPD010000292         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1176607, whole genome shotgun sequence.
ACCESSION   DASTPD010000292 DASTPD010000000
VERSION     DASTPD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176607"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFR57_09690"
     CDS             complement(<1..1112)
                     /locus_tag="VFR57_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0259675.1"
                     /translation="MPSTTTLARAPAAAAPRLAFDPKWLIIGVCVAVTVYLGIVPLGF
                     LLWQSFFTPHTAAKAAEFTLGNYREAYGSAETWVLFWNSVKFASGTAVLAFVLGTLLA
                     WMNERTNTPFKALFFALSLIPLVIPGILFTVSWILLGSPKIGLINLALQKVFDTEAVF
                     INVYSLWGMIWVDALHYSPMAFLLMTAAFRAMDPALEESAVMSGASVWQVLTRVTLPL
                     VWPAIFATLLILFVRAIESFEVPALLGLPVGIQVFTSAIYQAVHRYPSQIGLASAYAV
                     TLLLLTTIGVYFQSVLSSRGNKYATMTGKGFRPRQIDLGPWRYLAVAVFLVYFLLIVL
                     LPFAVLLWSSFQKFYSVPSIEAIQRMTFEPYRTVLD"
     gene            complement(1087..2667)
                     /locus_tag="VFR57_09695"
     CDS             complement(1087..2667)
                     /locus_tag="VFR57_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEU0259676.1"
                     /translation="MASNKPHTLADHVHGVDHDHDHDHDADHEHDASSEQNALWLQDN
                     VSLTSVGIDIGSAGTQVIFSRLHMRRMGEDLSSRYFVVNRETLYQSPVALTPYASETR
                     IDEEAVGRVIDEAYAAAGLHPDQVDTGSVILTGEALRSENAQAIGELLAEMGGEFVCA
                     TAGHHMEAMLAAYGSGAAQVSHDRGLRVLSIDIGGGTTKLALIEHGRVLETAAIHIGG
                     RLLVADAEGRITRLDPQGRALARQAGLDLQLGGRVAPAELDFLCEWMAESLCMAILGE
                     RRGYLTEPLRELRAVHGVMFSGGVGEYVYGREERDFGDLGRRLGRALAQRLDRLPGKL
                     LPAGECIRATAFGASEYSVQLSGNTVYVSEPGALLPRKNLPALQPPVRLEGPIDAATV
                     AAAIRAHFEAFDIAEGETEVALAFKWRGEPSFERLSTLARAITLALRATIEREKPIYL
                     VLEGDVAHTLGAILKEDWRLRSEVLVIDGVALRDFDYIDLGRIRVPSNTVPVTIKSLV
                     FSQDPRVPHRHAEHHHPR"
     gene            complement(2657..>3181)
                     /locus_tag="VFR57_09700"
     CDS             complement(2657..>3181)
                     /locus_tag="VFR57_09700"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0259677.1"
                     /translation="SGHGARRAGKVEQALGGPIHPEVTKARLSSVANSIGLLTSFSGE
                     EDELGITTLASRLRLAKSSVHRLATTLASAGFLEQNAETGKYRLGVALFALARSCVGA
                     WTWRTRRVRSCASCSRRPARRSSLASSTDFSVLYAPVQRMSKRMLHTCVPTVIDTASA
                     VSARLGYHRRPNGE"
BASE COUNT          588 a         1025 c         1060 g          508 t
ORIGIN      
        1 tgatcgagca cggtgcggta gggctcgaac gtcatgcgct ggatcgcttc gatggagggc
       61 acggagtaga acttctggaa cgacgaccac agcaggaccg cgaacggcag cagcacgatg
      121 agcaggaagt agaccaggaa caccgcgacc gcgaggtaac gccacgggcc gaggtcgatc
      181 tggcgcggcc ggaagccctt gccggtcatg gtcgcgtact tgttgccccg gctcgacagc
      241 accgactgaa agtacacgcc gatagtggtg agcagcagca gcgtgaccgc gtaggcggaa
      301 gcgaggccga tctggcttgg atagcggtgc accgcctggt agatcgccga ggtgaagacc
      361 tgaatgccga ccggaagccc gagcagcgcc ggcacctcga acgattcgat cgcccgcacg
      421 aaaagaatga gcaacgtggc gaagatcgcg ggccatacca atggcaatgt cacccgggtg
      481 agcacctgcc acacgctcgc gccgctcatc acggccgact cttcgagcgc cggatccatc
      541 gcgcggaacg ccgccgtcat cagcaggaaa gccatgggcg agtagtgcag cgcgtcgacc
      601 cagatcattc cccagagcga gtagacgttg atgaacaccg cttcggtgtc gaagaccttc
      661 tgcagcgcca ggttgatgag gccgatcttc gggctgccca gcaggatcca cgagacggtg
      721 aagagaattc ccgggatcac gagcggaatg agcgagagcg cgaaaaagag cgccttgaac
      781 ggggtattgg tgcgctcgtt catccacgcg agcagcgtcc cgagcacgaa agcgagcacg
      841 gcggtgcccg aggcaaactt gaccgagttc cagaacagca cccaggtttc cgccgagccg
      901 taggcctcgc ggtagttgcc gagcgtgaac tcggccgcct tggccgcggt gtggggcgtg
      961 aagaagctct gccagagcag gaagccgagc ggcacgatgc cgaggtaaac cgtgacggcg
     1021 acacagacgc cgatgatcag ccatttcggg tcgaaggcga gccgcggcgc agcggccgcc
     1081 ggcgccctag cgagggtggt ggtgctcggc atgccgatgc ggcacgcggg gatcctggct
     1141 gaagaccagg gacttgatcg tgaccggcac ggtattcgaa ggcacccgga tgcggccgag
     1201 atcgatgtag tcgaagtcgc gcagcgcgac gccgtcgatc accagcactt cggagcgcag
     1261 tctccagtct tccttgagga tcgcgccgag cgtatgcgcg acgtcgcctt cgaggacgag
     1321 gtagatcggc ttctcccgct caattgtggc gcgcagagca agggtgattg cgcgggccaa
     1381 cgtcgacagg cgctcgaacg aaggttcgcc gcgccacttg aacgcgagtg cgacctcggt
     1441 ttcgccttcg gctatatcga aagcttcgaa atgcgcgcgg atggccgcag ccacggtcgc
     1501 ggcgtcgatc ggcccttcga gccgtacggg cggctgcagc gccggcaggt tcttgcgcgg
     1561 cagcagcgcc cccggctcgg acacatatac cgtgttgccg gaaagctgaa cgctgtactc
     1621 ggaggcgccg aacgccgtcg cgcggatgca ctctccggcg ggaagcagtt tccccggcag
     1681 gcggtcgagg cgctgcgcga gcgcccggcc cagtcgcctg cccaggtcgc cgaaatcgcg
     1741 ctcctcgcgt ccgtagacgt attcaccgac cccgccggaa aacatgacgc cgtggacggc
     1801 gcgcagctcc ctgagcggct cggtgagata gccgcgccgc tcgcccagta tcgccatgca
     1861 gagcgattca gccatccatt cgcacaagaa atccagctca gccggtgcga cccggccgcc
     1921 gagctgtaaa tcgagccctg cctgccgtgc cagcgcgcgc ccttggggat caaggcgtgt
     1981 gatgcgaccc tcggcgtccg cgacgagcag ccggccgccg atgtggatcg cggccgtctc
     2041 gaggacgcgg ccgtgctcga tcagggcgag cttggtggtg ccgccgccga tatcgatgct
     2101 gagcacgcgc aggccgcggt cgtgcgatac ctgcgccgcg cccgagccgt acgccgcaag
     2161 catcgcttcc atgtgatgcc cggcggtcgc gcaaacaaac tctccgccca tctcggcgag
     2221 cagctcgccg atcgcctggg cattctcgct gcgcaacgct tcgccggtca ggatcaccga
     2281 gccggtatcg acctggtcgg gatgcaggcc ggcggccgcg tacgcctcgt cgatgacgcg
     2341 gccaacggcc tcttcgtcga tgcgcgtctc gctcgcataa ggcgtgagcg ctaccggcga
     2401 ctggtagagc gtctcgcggt tgacgacgaa atagcggctc gacaggtcct cccccatgcg
     2461 tcgcatatgc agccgcgaga agatcacctg ggtaccagcc gagccgatgt cgatgcccac
     2521 gcttgtcagc gagacgttgt cttgcagcca gagcgcgttt tgctcggacg aagcatcgtg
     2581 ctcgtggtcg gcgtcgtgat cgtgatcatg atcgtgatcc accccgtgaa cgtggtcggc
     2641 gagtgtatgg ggcttgttac tcgccattgg ggcgtcggtg gtacccgaga cgcgccgaaa
     2701 cggcgcttgc ggtgtcgatg acggtgggca cgcacgtgtg cagcatcctt ttgctcatgc
     2761 gctgtacagg cgcgtagagc acgctgaaat cggtcgacga tgccaagctg gaccgtctcg
     2821 ccggtcttct cgagcagctc gcgcagcttc ggacgcgcct cgttcgccac gtccatgcgc
     2881 cgacgcacga gcgcgccagc gcaaagagcg ctacgccgag ccggtacttc ccggtttcgg
     2941 cgttctgctc gagaaacccc gcgctcgcca gcgtcgtggc gagacgatgg acgctgctct
     3001 tcgctaagcg cagccgactg gcgagcgtgg tgatgccgag ctcgtcctcc tcgccggaaa
     3061 acgacgtcaa caaaccgatc gagttcgcga ccgaggacag ccgcgccttc gtcacttccg
     3121 gatgaatagg ccctcccaga gcctgttcca cttttcctgc tcgtcgagca ccgtgaccgg
     3181 a
//
LOCUS       DASTPD010000293         5768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1789810, whole genome shotgun sequence.
ACCESSION   DASTPD010000293 DASTPD010000000
VERSION     DASTPD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789810"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFR57_09705"
     CDS             complement(<1..1227)
                     /locus_tag="VFR57_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259678.1"
                     /translation="MRATCATCVRFSASSLPKRSSTAAAVLAACFVLNMFGRGLGDTY
                     AVFLLPLERDFGWTRSQLTSVYSIYLLVNGCTAPFVGLIFDRFGPRWVYGTGMASLGA
                     AFFLASGLTVLWQFYLFIGVLVGIGVSMNGMVPASALLGRWYRERLSSAIGIAYSAFG
                     VGTVLFVPLVQYLVGAFDWRATYRFMGLALLLAAPVVVIGLPWRRFAAGHPASRPQAK
                     GKHGAGWTLATAVRTPVFWGLVQVFGCTAAAMFSIVVQLVAFLVDAGFPPLSAATAFG
                     IVGLLSAASVMGSGFASERFGYRQTVTASFAGTAAGMLTLLAITAYPLTILLAVFVAV
                     FGLCMGVRGPIVSSVCARYFAGPRVATIYGLIYASNAVGAALGSFAGGLLHDLTGGYR
                     AGLAMALVLIAVAAMPF"
     gene            complement(1166..2350)
                     /gene="pncB"
                     /locus_tag="VFR57_09710"
     CDS             complement(1166..2350)
                     /gene="pncB"
                     /locus_tag="VFR57_09710"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU0259679.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAQVEYKFRCRTEGVDLRS
                     DLDRIERELAELCRLRFRDEELAYLRGLRFMKSDFVDFLALFQFNEKYVGTGLGEKPG
                     EIDITIRGPWLHTILYEIPVLAIVSELYFRRTQPRPNLDEGRRRLKAKIDLVRTVEPE
                     LDFKISDFGTRRRFSLAWHEEVVQALKREVPQYFAGTSNVWFAMRNQLTPLGTMAHEY
                     MQACQALGPRLRDSQTFAFDKWAQEYRGDLGIAIADTYGLEAFLRDFDMYFCKLFDGA
                     RHDSGDPFEWGEKMIAHYRANRVDPRTKTLIFSDQLSFPLAIEIARRFYGRARTSFGI
                     GTNLTNDLGYEPINIVLKMTECNGQPVAKVSDSPGKTISKDPGYLRYLRQVFGLEPAE
                     KI"
     gene            2389..2970
                     /locus_tag="VFR57_09715"
     CDS             2389..2970
                     /locus_tag="VFR57_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEU0259680.1"
                     /translation="MCGRYALHANPEVLALQFGLDSVPEFRPSYNIAPAAEILVVRPG
                     TAARARWGLRGRFVNLRAETVLSRFGASGRCLVPASGFYEWKAEGRRKQPYYFSPPRN
                     ALLALAALWERDTCSLITTDPNPVVRAVHDRMPLMVPRSLYQAWLHGDETILRSAPEI
                     ELRAHPVSMAVNQAANDSPEMIEPVELARGLFD"
     gene            complement(2947..3987)
                     /gene="dinB"
                     /locus_tag="VFR57_09720"
     CDS             complement(2947..3987)
                     /gene="dinB"
                     /locus_tag="VFR57_09720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0259681.1"
                     /translation="MRRVVHLDMDAFYASVEQRDQPELRGKPVAVGGRPQARGVVAAA
                     SYEARAFGVRSAMPMARALRLCPELMIVSPDFERYRRVSSQVMAILRACTPLVEPLSL
                     DEAYLDVTENAWGEEFGSRVARRLKQQIVEQTGLTASAGVAPNKFLAKIASGYRKPDG
                     LTVISPERVESFLQELPVEALWGVGPVTARKLRGLGIERLVQIRTADAQLLRDTVGSL
                     ADWLRQLSQGDDPRPVVPDRESKSSSTENTYAHDLVDADAIRAELDRMARENADWLAR
                     RRLAARTVTIKVRYSDFKTVTRSHTLSRPTADADLIASWAVELAARTQAGMKAVRLLG
                     VRVQGLVEQAAG"
     gene            4007..4636
                     /gene="lexA"
                     /locus_tag="VFR57_09725"
     CDS             4007..4636
                     /gene="lexA"
                     /locus_tag="VFR57_09725"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU0259682.1"
                     /translation="MDGLTERQAQILRLVRELTEVSGFPPTRAEIAERMGFRSVNAAE
                     QHLRALEKKGAIEISSGASRGIRVRDGRPAARLGRLLELPLVGRVAAGSPILAEENLQ
                     GRYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHKTSEARSGQIVVARLADEV
                     TVKRYRRRGHTVELEAENPDYKPIEIDLRREPLTIEGTAVGVIRNGKAL"
     gene            4674..>5768
                     /locus_tag="VFR57_09730"
     CDS             4674..>5768
                     /locus_tag="VFR57_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0259683.1"
                     /translation="MNHTTTADGSAARLEVSTDAVEAEIRDRADPQQVLADRVAQLYR
                     QMPIAIGATFIAGAIATFELQGRWLKELVLIWWAIVVTFTAAASLLLYAYYRSPDKVA
                     SAPQWLRWLGIAALGNGASWGLAGGVFFRSLSDEQQVFLAFLFAGMASVGIPVYAASW
                     PIFALYAAGILGPFFYVLLTFGNRLFVEIALLVPLFYAINVAIAYRLTQVFHSGYRLR
                     HAYGRLTRDHRQLNQRLEQQLVELEEARRQVEASGRKLALFAERAPIAVLELQPDGTV
                     VEVNHAAEILFGYAAAELVGGPVKKLVLPKFHEDFDHTWQQLVSSRESVAGLKIRNPR
                     RDGAEIICEWTVTPLVNFQREVIAVIAQGRD"
BASE COUNT         1021 a         1930 c         1906 g          911 t
ORIGIN      
        1 gaatggcatg gctgcgacgg cgatcagcac gagcgccatt gcgaggccgg cgcgataccc
       61 gcccgtcaga tcgtgcagca ggccgcccgc gaatgaaccc agcgccgcgc caacggcgtt
      121 gctggcatag ataaggccgt agatcgtggc cacccgaggg cccgcgaaat agcgggcgca
      181 gaccgacgac acgataggtc cccgtacccc catgcatagg ccgaagactg cgacgaacac
      241 cgcgagcaat atcgtcaaag ggtacgccgt gatcgcaagc agggtcagca tgccggctgc
      301 cgtgccggca aagcttgcgg tgaccgtctg ccggtagccg aagcgctcgg acgcgaagcc
      361 gctgcccatc acactcgccg cggatagaag cccaacgatg ccgaacgcgg tggcggcgct
      421 gaggggtggg aagccggcat cgaccagaaa cgccacgagc tgcaccacga tggagaacat
      481 ggcggccgcg gtgcagccga aaacctgaac gaggccccag aacaccggcg tgcgtacggc
      541 cgtagcgagc gtccagcccg ccccgtgttt tccttttgcc tgtgggcggg aggccgggtg
      601 tcccgcggca aacctgcgcc agggtagccc gatcaccacc acgggcgcag caagcagtag
      661 cgcaagaccc atgaatcgat aggtcgcgcg ccagtcgaac gctccgacca gatactggac
      721 gagcggcacg aaaagcacgg tacccacgcc aaaggccgag tacgcgatcc ctatggcgct
      781 cgagagccgc tcgcggtacc agcgaccgag gagcgcggac gcgggaacca tgccattcat
      841 gctgacgccg atgccgacca gcacgccgat gaagagatag aactgccaca gcacggtgag
      901 cccgctcgcg aggaagaacg ccgcgccgag gcttgccatc ccggtgccgt agacccagcg
      961 cggcccaaac cggtcgaaga tgagccccac gaacggcgcg gtgcagccgt tcaccaggag
     1021 gtagatcgaa tagacgctcg tcaactgcga gcgggtccag ccgaaatcgc gctcgagcgg
     1081 gagcaggaat accgcgtagg tgtcgcccag gccccgcccg aacatgttca ggacgaagca
     1141 ggccgcgagg acggcggccg cggtactaga tcttttcggc aggctcgagg ccgaaaacct
     1201 gacgcaggta gcgcaggtag cccggatcct tgctgatcgt cttgccgggc gagtccgaca
     1261 ccttggcgac cggctggccg ttgcactcgg tcatcttgag cacgatgttg atcggctcgt
     1321 agccgaggtc gttggtgagg tttgtaccga tgccgaacga cgtgcgcgca cggccgtaga
     1381 aacgtcgcgc gatctcgatc gcaaggggaa acgagagctg atcggagaag atgagcgtct
     1441 tggtgcgcgg atcgacgcgg ttcgcgcggt aatgagcgat catcttctcg ccccactcga
     1501 agggatcgcc cgagtcgtgc cgcgccccgt cgaaaagctt gcagaagtac atgtcgaagt
     1561 cgcgcaggaa ggcctccagc ccgtacgtat cggcgatcgc gatgccgagg tcgccgcggt
     1621 attcctgcgc ccatttgtcg aaggcgaagg tctgcgagtc gcgcagccgc ggtccgagcg
     1681 cctggcaggc ctgcatgtac tcgtgggcca tggtgccgag cggcgtgagc tgattgcgca
     1741 tggcgaacca gacgttggag gtgccggcga agtactgcgg gacctcgcgc ttgagcgcct
     1801 gcaccacctc ctcgtgccac gcgagcgaga agcgccggcg cgtgccgaag tcggaaatct
     1861 tgaagtcgag ctcaggctcg actgtgcgca cgagatcgat cttcgccttg aggcgccgcc
     1921 ggccctcgtc gaggttcggg cgcggctgcg tgcgccggaa gtacagctcc gacacgatcg
     1981 cgagcaccgg gatctcatag agaatggtat gcaaccacgg gccgcggatc gtgatgtcga
     2041 tctcgcccgg cttttcgccc agtcccgtgc caacgtactt ctcgttgaac tggaacaggg
     2101 ccaggaagtc gacgaagtcg gacttcatga aacgcaggcc gcgcaggtaa gcaagctcct
     2161 cgtcgcggaa gcgcaggcgg cagagctcgg cgagctcgcg ttcgatgcgg tcgaggtcgg
     2221 agcgcaggtc cacgccctcg gtgcggcacc tgaacttgta ttcgacctgc gcgccaggga
     2281 agtggtgcag cacgacctgc atcatggtga acttgtacag gtcggtgtcg agcagcgact
     2341 ggatgatcat tgcgcgcaaa ttgtacccgg ggcggttatc ctcccggcat gtgcggccgc
     2401 tacgcgctgc atgcgaatcc cgaggtgctg gcgctgcagt tcgggctcga ttcggtgccc
     2461 gaattcaggc cgagctacaa catcgcgccc gcggctgaaa tcctcgtagt gcggcccggg
     2521 actgcggcgc gcgcgcgctg gggcctgcgc ggccggttcg tcaacctgcg cgcggaaacc
     2581 gtgctctcaa gattcggagc gagcggccgc tgcctcgtgc cggcgagcgg cttctacgaa
     2641 tggaaagccg aaggccggcg caagcagcct tactactttt cgcccccgcg caatgcgctg
     2701 ctcgcgctcg ccgctctgtg ggaacgggat acctgctcgc tcattactac ggatccgaac
     2761 ccggtggtac gcgccgttca cgatcgcatg cccctcatgg tgccaaggag cctttaccaa
     2821 gcgtggctgc acggcgacga aaccatcctc cggtcagcgc ccgagatcga gctgcgcgct
     2881 catccagtca gtatggcggt aaaccaggcc gcgaacgatt cgccggagat gatcgagccg
     2941 gtcgagctag cccgcggcct gttcgactaa tccctgcacg cgcacaccga ggagccgcac
     3001 cgccttcatt cccgcctgag tgcgcgcggc gagctccacc gcccacgagg caatgaggtc
     3061 cgcatccgct gtcgggcgcg acagcgtatg actgcgcgtc acggtcttga aatcggaata
     3121 gcgcaccttg atcgtcacgg tgcgcgctgc gagccgccgc cgcgcgagcc agtcggcgtt
     3181 ctcgcgcgcc atgcgatcga gctcggcgcg gatggcatcc gcatcgacca gatcgtgcgc
     3241 atacgtattc tccgtgctgg aagacttcga ctcgcggtcc ggcaccaccg gccgcggatc
     3301 gtcgccttgc gagagctggc gcagccagtc ggcgagactg cccacggtgt cgcgcaggag
     3361 ctgcgcatcc gcggtgcgga tctgcaccag ccgttctatc ccgagtccgc gcagcttgcg
     3421 ggcagtcacc ggccccactc cccacagcgc ttccaccggc agctcctgca ggaagctctc
     3481 cacgcgctca ggcgagatca cggtaagccc gtcgggcttg cggtagccgg aggcgatctt
     3541 ggccaggaac ttgttcggcg cgacgcccgc cgaagcggtg agccccgtct gctcgacgat
     3601 ttgctgcttc agccgtcggg cgacgcggct accgaactcc tcgccccagg cgttctcggt
     3661 gacgtccaga taagcctcgt cgagcgaaag cggctccacc agcggcgtgc aagctcgcag
     3721 aatggccatc acctggctcg acacgcgccg gtagcgctcg aagtccggag acacgatcat
     3781 gagctcgggg cagagtctca gcgcgcgcgc catcggcatc gccgagcgca cgccgaacgc
     3841 gcgcgcctcg tagctcgcgg ccgccaccac gccgcgcgcc tgcggccggc ccccgaccgc
     3901 caccggcttt ccgcgcagtt ccggttggtc gcgttgctcg accgaggcgt agaacgcgtc
     3961 catgtccaga tggacaaccc gccgcatact gtatatattg ccactaatgg acggacttac
     4021 agagcggcaa gcgcagatcc tgcgcctcgt ccgggagctg accgaggtct cgggttttcc
     4081 gccgacccgc gccgagatcg ccgagcgcat gggctttcgc tcggtgaacg ctgccgagca
     4141 acacctgcgc gcgctggaga aaaagggcgc gatcgaaatc tcttccggag cctcgcgcgg
     4201 catccgggtg cgcgatggcc gccctgccgc ccgcctcggg cggctgctcg agctgccatt
     4261 ggtcggccgc gtcgccgcgg gcagcccgat cctcgccgag gagaacctgc agggccgcta
     4321 tcaggtcgat cccaatctgt tcacgccgcg cgccgactac ctgttgcgcg tgcgcggcat
     4381 gtcgatgcgc gatgcgggaa tcctcgaggg cgacctgctc gccgtgcaca agacgagcga
     4441 agcgcgcagc gggcagatcg tggtcgcccg gctcgccgac gaggtcaccg tgaagcgcta
     4501 tcgccggcgc gggcatacgg tggagctcga agcggagaat ccggattaca agcccatcga
     4561 gatcgacctg cggcgtgagc cgctcaccat tgaaggaacc gccgttggcg taatacgcaa
     4621 cggcaaggcc ctctaagaca tcttagaatg gcgctggccc caggcccgcg ccgatgaatc
     4681 acactacaac agcagacggc agcgccgccc gactggaggt ctcgaccgat gcggtcgagg
     4741 ccgagatccg cgatcgcgcc gacccgcagc aggttctcgc cgaccgcgtc gcccagctct
     4801 accgccagat gccgatcgcc atcggcgcga ccttcatcgc cggcgcgatt gcgaccttcg
     4861 agctgcaggg ccggtggctc aaggagctgg tgctgatatg gtgggcgatc gtcgttacct
     4921 tcaccgcagc ggcgagcctg ctgctctatg cctattaccg aagcccggac aaggtcgcga
     4981 gcgcgccgca atggctgcgc tggctcggca tcgcggcgct cggcaacggc gcaagctggg
     5041 ggcttgcggg cggtgtgttc ttccgttcgc tgtcggacga gcagcaggtc ttcctggcgt
     5101 ttctcttcgc cgggatggct tcggtgggca tcccggtgta cgccgcgtcg tggccgatct
     5161 tcgcgcttta cgcggcaggc attctgggcc cgtttttcta cgtgctcctc actttcggca
     5221 atcgcctgtt cgtggagatc gcgctgctgg tgccgctctt ctacgcgatc aacgtcgcta
     5281 tcgcttaccg gctgacgcag gtgttccact ccggataccg gctgcggcac gcctacggca
     5341 ggctcacccg ggaccaccgc cagctcaacc agcgcctcga gcagcagctg gtcgagctgg
     5401 aggaggcgcg gcgccaggtc gaggcgagcg gccgcaagct cgccctcttc gccgagcgcg
     5461 cgccgatcgc ggtgctcgag ctccagcccg acggcacggt ggtggaggtg aaccatgccg
     5521 ccgaaattct gttcggctac gccgccgccg agctcgtcgg cgggccggtc aagaagctcg
     5581 tgctgcccaa gtttcatgag gacttcgacc acacgtggca gcagcttgtc agctcgcgcg
     5641 aatcggtggc cggcctcaag atccgcaacc cgcgccgcga cggcgccgag atcatctgcg
     5701 aatggaccgt caccccgctc gtcaacttcc agcgggaggt gatcgcggtg attgcccagg
     5761 ggcgcgac
//
LOCUS       DASTPD010000294         9122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_156085, whole genome shotgun sequence.
ACCESSION   DASTPD010000294 DASTPD010000000
VERSION     DASTPD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156085"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFR57_09735"
     CDS             complement(<1..1052)
                     /locus_tag="VFR57_09735"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0259684.1"
                     /translation="MADVSQLKRLRPTSCQVPVSWYFDPEIFEAERRLLFQAGPNYVG
                     HELMVPNAGDYQTLAWTDHAKVLVNNGNGIELLSNICRHRQAIMLEGKGNAQNIVCPV
                     HRWTYDMKGELLGAPRFENQPCVKLHSTPLQRWNGLLFTGPRNPAADLAKITTIADWD
                     FSGFVLDSVRIDEYDINWKTFIETYLEVYHVDFFHPGLGNFTDCDNFTVDYGADYSVQ
                     IVQAKAALAKPGTPTYKRWHEACLKYLDGREPKYGALWMTYFPGLMLEWYPHVLIVSH
                     LIPRSPTKTTNVVEFYYPEEIALFEREFVEAQQAAYVETALEDNEICLRLDRGRRALY
                     ESGMDDAGPYQSPMEDA"
     gene            1093..1347
                     /locus_tag="VFR57_09740"
     CDS             1093..1347
                     /locus_tag="VFR57_09740"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0259685.1"
                     /translation="MSEPSAELEPLEFEKALAELEKIVARMESGELSLEQALATHKRG
                     LELARYCQQKLEAAQQQVKVLEGEVLKPLAAAAQRDEDPR"
     gene            1331..2230
                     /locus_tag="VFR57_09745"
     CDS             1331..2230
                     /locus_tag="VFR57_09745"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU0259686.1"
                     /translation="MKILDSEFQAWMGSVQARMESVLARVLPAAHLVPARLHDAMRYS
                     TLEGGKRVRPLLAFAAGEVSGAAPERLEIAAAAVECIHVYSLVHDDLPCMDDDTLRRG
                     KPTVHVEYDEATALLVGDALQSLAFQLLAEHRLADDARIQLDMVKTLAQAAGSRGMAG
                     GQQIDLEATGKPLTLPQLELMHIHKTGALIRAAVRLGAACGALPKEDERKLDRYAKAI
                     GLAFQVVDDVLDYDASTATLGKTAGKDSQRGKPTYVSALGLSRAREFAEELRAEAHVS
                     LEGIGAPARRLGELADFIVLRKF"
     gene            2234..4111
                     /gene="dxs"
                     /locus_tag="VFR57_09750"
     CDS             2234..4111
                     /gene="dxs"
                     /locus_tag="VFR57_09750"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU0259687.1"
                     /translation="MAAITHELPADGGNPPSYELLKTIDDPAQLRALDRKQLAQLATE
                     LRAFVLESVSKTGGHLSSNLGTVELTIALHYVFDTPEDRIVWDVGHQTYPHKILTGRR
                     EAMARLRMLDGISGFPRRAESRYDTFGTAHSSTSISAALGMAVGAKLKKEKKKCIAVI
                     GDGAMSAGMAYEAMNNAGAMDVDVLVILNDNEMSISPPVGALTNSLARLLSGRVYSPA
                     RRASEHLLKRVGVWELARRTEEHMKGMVTPSTLFEEFGFNYIGPIDGHDLDALIPTLG
                     NIRKLAGPQFLHVITRKGQGYKLAEEDPIAYHGPSKFDPAVGITPTAGARTTYSQVFG
                     EWLCDMAAREPRLVAITPAMREGSGMVAFSERYPERYFDVGIAEQHAVTFAAGLACEG
                     MRPVVAIYSTFLQRGYDQLIHDVAIQNLPVLFALDRGGLVGGDGATHNGAFDYAYLRT
                     VPNLTIMAPSDAAECRNMLYTGANLNGPAAVRYPRGAALGDPGREMCALSVGKAEIRR
                     AGKRVAILAFGSMLKPALEAGEALDATVVNMRFVKPLDADVVRELARTHELLVTVEEH
                     QVMGGAGSAVCEVLGGETKVLLLGLPDRFIDHGDPGRLLASVGLDAAGIQQAIRDTMS
                     E"
     gene            4122..4925
                     /gene="folE2"
                     /locus_tag="VFR57_09755"
     CDS             4122..4925
                     /gene="folE2"
                     /locus_tag="VFR57_09755"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEU0259688.1"
                     /translation="MKARDLQNIPDVQASPDSRKLAIDKVGVKAIRHPMKIQERAGGV
                     QHTVATFNMYVCLPHHFKGTHMSRFVQILEEHEREITVDSFQAMLREMVEKLEAEEGH
                     IEMSFPYFIEKKAPVSGVRSLLDYEVTFLGEYRKKNGRFTMKVVVPVTSLCPCSKKIS
                     ERGAHNQRSHVTVTARTHEFVWIEEIVDIVERQASSELYGLLKRPDEKFVTERAYDNP
                     KFVEDMVRDVAAVLNLDERIDAYVVESENFESIHNHSAYALIERNKRGR"
     gene            4943..5629
                     /locus_tag="VFR57_09760"
     CDS             4943..5629
                     /locus_tag="VFR57_09760"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU0259689.1"
                     /translation="MLGFSADAQDLPAQRARMVSEVQAMYAETQRETGFAAMSNEVAR
                     ALGKVERHRLVPPGEASRAYRNHPLPIGSGQTISQPYIVALSTDLLRPKPTDTVLEVG
                     TGSGYQAAVLAEIVSTVYSIEVIPALGNEARRRLAELGYRNIEVRIGDGYAGWPEKAP
                     FDGIVVTAAAPRVPKALVDQLKPGGRMVIPVGGEMEIQYLNVLTKRADGGVDDKRVLP
                     VRFVPLVPGK"
     gene            complement(5630..6268)
                     /locus_tag="VFR57_09765"
     CDS             complement(5630..6268)
                     /locus_tag="VFR57_09765"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEU0259690.1"
                     /translation="MARIIALVLAFAFAFTCAARDDVALVGLIGDKAAVLAVDGGDPK
                     TVRVGQKWNGITVITVERGQAIVEVDGKRRTLTIGQHYRSVDPRSDRQSITVAADPRG
                     HFFAEGAVNGNSVRFLVDTGATTVALPAADAVRLGLDYRKGRSGFTHTAAGVVPVFVV
                     TLDTVRLGGIELNSVEATVIERGLDVALLGMSFLNRVEMRREGHLMTLTRRF"
     gene            6267..6977
                     /locus_tag="VFR57_09770"
     CDS             6267..6977
                     /locus_tag="VFR57_09770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU0259691.1"
                     /translation="MRPVAIFRAAPTEGPGYFAAYLERASIPWKLVALDAGEAVPRDP
                     REFSGLGIMGGPMSANDDLPWIPPLLEFLRDAVRRDVPIIGHCLGGQLMSRAFGGEVR
                     VNPVREIGWGEVRVADNGVAREWLGDLQSFQTFQWHGDTFSIPPGATRVLEGEHCANQ
                     AFAIGRHLGMQCHVEMTPELVRTWLSTGQKEIVEHGKSPAVQSPAEIEKDLDTRLERL
                     HEIADRIYDRWTENLSRG"
     gene            complement(7000..7485)
                     /locus_tag="VFR57_09775"
     CDS             complement(7000..7485)
                     /locus_tag="VFR57_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU0259692.1"
                     /translation="MPSFDIVSQVDRQEVKNAVEQTNKEITNRFDFKGSDARVEQNEL
                     ALTVYADDEFKLGQVLDVLRQRLAKRNVDVRALESGTIEKISGDKVKRPVTVKVGVPQ
                     EKAKHIQKLIKDSKIKVAASIQGDAVRVSGGKKDDLQAAIQLVRKSVTDLPLQYINFR
                     D"
     gene            complement(7496..8146)
                     /locus_tag="VFR57_09780"
     CDS             complement(7496..8146)
                     /locus_tag="VFR57_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259693.1"
                     /translation="MRSILVSMLLAASSALAAPFAVQLGDARVALDAPPGFADTQFTG
                     SPRLQEIGESLTSASNRVLLFALSDADLRRFMLGDQLELRRYMIAVTPRAMERERLSE
                     GAFKSLAGNVRGEVTDLPTGDFQKHLDSRAPGTVVMLAELRKEADAVSVLQGSRSKAG
                     MFERTRYLLTSNSLVLLRGRALTLSVYTQYDDPADLEWIRSTTNRWIDDLKRLNAR"
     gene            8194..>9122
                     /gene="argG"
                     /locus_tag="VFR57_09785"
     CDS             8194..>9122
                     /gene="argG"
                     /locus_tag="VFR57_09785"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0259694.1"
                     /translation="MAKILGSLPQGEKIGIAFSGGLDTSAAVHWMRAHGAIPHAYTAN
                     LGQPDEDDYDAIPKKALAYGAEKARLVECRPQLVAEGIAAIQCGAFHISTGGATYFNT
                     TPLGRAVTGTMLVLAMREDEVGIWSDGSTYKGNDIERFYRYGLLANPALRIYKPWLDQ
                     RFIDELGGRKEMSAYLAKAGFDYKTSAEKAYSTDSNILGATHEAKDLEFLDKGIAIVE
                     PIMGVAFWREDLRIKNEKVTVEIAEGAPVALNGQRFSDPVKLMEEANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTG"
BASE COUNT         1650 a         2919 c         2999 g         1554 t
ORIGIN      
        1 gcgtcttcca tgggcgactg atacggaccg gcgtcgtcca tgccgctctc ataaagcgcg
       61 cgccgtccgc ggtcgaggcg caggcagatc tcgttgtcct cgagcgccgt ctcgacgtat
      121 gccgcctgct gggcctcgac gaactcgcgc tcgaagagcg cgatctcttc ggggtaatag
      181 aactcgacca cgttcgtcgt tttggtcggc gagcgcggaa tgagatgcga aacgatcagc
      241 acgtgcgggt accactccag catgaggccc gggaaatacg tcatccacag cgcgccgtac
      301 ttcggctcgc ggccatcgag atacttgagg caggcctcgt gccagcgctt gtacgtcggc
      361 gtgccgggct tggcgagcgc cgctttggcc tggacgattt gcaccgagta gtccgctccg
      421 taatccacgg tgaagttgtc gcagtcggtg aagttgccca gtcccggatg aaagaagtcg
      481 acgtggtaga cctcgagata ggtctcgatg aaggtcttcc agttgatgtc atattcgtcg
      541 atgcggaccg aatcgagtac gaagccgctg aaatcccagt cggctatcgt ggtgattttg
      601 gcgaggtcag cggcgggatt gcgcgggccc gtgaaaagca ggccgttcca gcgctggagc
      661 ggcgtggagt gcagcttgac gcagggctgg ttctcgaagc gcggagcgcc gaggagctcg
      721 cctttcatgt cgtaagtcca gcggtgcacg gggcacacga tgttctgagc gttgcccttg
      781 ccctcgagca tgatggcctg gcggtgccgg cagatgttcg aaagaagctc gatgccgttg
      841 ccgttgttga cgaggacctt ggcgtggtcg gtccaggcga gcgtctggta atcccccgcg
      901 ttcggcacca tgagctcgtg gccgacgtag ttggggccgg cctggaacag aagccgcctt
      961 tccgcttcga aaatctccgg atcgaagtac caggagactg gtacctggca gcttgttggt
     1021 cttagccgct tgagctgcga aacatccgcc atgcgttgta aagctcctag aaaaacagta
     1081 tcttaccaaa agatgagcga gccttcagca gagctcgagc ccctcgagtt cgaaaaggcg
     1141 ctggccgagc tcgaaaaaat cgtcgcgcgc atggaaagcg gcgagctttc gctcgagcag
     1201 gcgctcgcca cgcacaagcg cgggctcgag ctcgcgcgtt actgccaaca gaagctcgag
     1261 gcggcgcagc agcaggtcaa ggtgctcgag ggcgaagttc taaagcccct ggcggccgcc
     1321 gcgcagcgcg atgaagatcc tcgatagcga gtttcaggcc tggatgggca gtgtgcaggc
     1381 ccgtatggaa agcgtcctgg cacgcgtgct gccggcggcg catctcgtgc cggcgcggct
     1441 gcacgacgcg atgcgctatt cgacgctcga gggcggcaaa cgcgtgcggc cgctcctcgc
     1501 ctttgcggcc ggcgaagtgt ccggcgcggc acccgagcgc ctcgagatcg ccgccgccgc
     1561 ggtggaatgc atccacgtgt actccctcgt gcacgacgat ctaccgtgca tggacgatga
     1621 cacgctgcgc cgcgggaaac cgaccgtgca cgtggagtat gacgaagcga ccgcgctcct
     1681 cgttggcgat gcgctccagt cgctcgcctt ccagcttctc gccgaacatc ggctggccga
     1741 cgatgcacgc atccagctcg acatggtgaa aacccttgcg caggcggccg gctcacgcgg
     1801 catggcgggt gggcagcaaa tcgacctcga ggcgacgggc aaaccgctca ctttgcccca
     1861 gctcgaactc atgcatatcc acaaaaccgg tgcgctgatc cgtgccgcgg tcaggctggg
     1921 cgcggcgtgc ggcgcgctgc cgaaagagga cgagcgcaag ctcgaccggt atgccaaggc
     1981 gatcggtctc gcattccagg tggtggacga cgtgctcgac tacgacgcga gcacggcaac
     2041 gctcggcaag accgcgggca aggattcgca gcgcggcaag ccaacttatg tatcggcgct
     2101 cggcttgagc cgcgcgcgcg aattcgcgga ggagctgcgc gccgaagcgc acgtttcgct
     2161 ggaaggaatc ggcgcgccgg caaggcgcct cggcgagctc gccgacttca tcgttttgcg
     2221 gaagttctag gcaatggcgg cgatcacgca cgagctgccg gccgacggcg gtaacccgcc
     2281 gagctatgag ctcctcaaga cgatcgacga tccggcgcag ctgcgcgcac tcgaccgcaa
     2341 gcagctcgcc cagcttgcaa ccgagcttcg agccttcgtg ctcgagtcgg tctcgaagac
     2401 cggcggacat ctctcgtcca atctcggcac cgtcgagctc acgatcgcgc tgcattacgt
     2461 gttcgacacc ccggaagacc gcatcgtctg ggatgtcggc caccagacct atccgcacaa
     2521 gattctgacc ggacggcgcg aggcgatggc gcggcttcgc atgctcgacg gcatctcggg
     2581 cttcccgcgc cgcgccgaat cgagatacga caccttcggc accgcgcact cgagcacatc
     2641 gatctccgcc gcgctcggca tggccgtcgg cgcgaagctc aagaaggaga agaagaagtg
     2701 cattgccgtg atcggcgacg gcgccatgtc ggccggcatg gcgtatgagg cgatgaacaa
     2761 tgccggcgcg atggatgtcg acgtgctcgt catcctgaac gataacgaga tgtcgatctc
     2821 gccgccggtc ggtgcgctga cgaattccct cgcgcggctc ctatccggcc gggtctattc
     2881 ccccgcgcgg cgcgcgagcg agcacctctt gaagcgcgtg ggcgtttggg aactcgcgcg
     2941 tcgcaccgag gagcacatga agggcatggt gaccccttcg acgcttttcg aggagttcgg
     3001 cttcaactac atcggtccga tcgacgggca tgacctcgac gcgctcatcc ctacgctcgg
     3061 caacattcga aagctcgcag gcccgcagtt cctgcatgtc atcacgcgca aggggcaggg
     3121 ctacaagctc gccgaagagg atccgatcgc ctaccacggg ccgagcaagt tcgatccggc
     3181 ggtcggcatc accccgacgg ccggtgcacg cacgacgtac tcgcaggtgt ttggcgaatg
     3241 gctgtgcgac atggccgcgc gcgagccgcg cctcgtcgcc atcactccgg cgatgcgcga
     3301 aggctcggga atggttgcct tttccgagcg ctaccccgag cgctacttcg acgtcggcat
     3361 cgccgagcag cacgcggtta cgttcgcggc aggactcgcc tgcgaaggca tgaggcccgt
     3421 cgtcgccatc tattcgacgt tcctgcagcg cggctacgac cagctgatcc acgacgtcgc
     3481 gatccagaac cttccggtcc tctttgccct cgatcgtggt gggctcgtcg gcggcgacgg
     3541 cgccacgcat aacggtgcct tcgactatgc ctacctgcgc acggtgccca acctcaccat
     3601 catggcgcct tcggacgcgg ccgaatgccg caatatgctt tacaccggcg ccaacctgaa
     3661 cggcccggcg gcggtgcgtt atccgcgcgg cgccgcgctc ggcgatcccg ggcgcgagat
     3721 gtgtgcgctc agtgtaggca aggcggagat ccgccgtgcc gggaaacgcg tcgcgatcct
     3781 cgcgttcggc tcgatgctga agcccgcgct cgaggcgggc gaggccctcg acgcgaccgt
     3841 ggtcaacatg cgcttcgtga agccgctcga cgcggatgtg gtgcgcgagc tggcgcgcac
     3901 gcacgagctt ctcgtcactg tcgaagagca ccaggtgatg ggcggcgcgg gttccgcagt
     3961 gtgtgaagtc cttggcgggg aaacgaaggt gctgctcctc gggctgcccg accgtttcat
     4021 cgatcacggc gacccgggca ggctgcttgc atccgtgggg ctcgacgccg cgggaatcca
     4081 gcaagccatc cgggacacga tgtccgagta aaatcggggc catgaaagcc cgggacctgc
     4141 agaacattcc cgacgtccag gcaagccccg attcgcgcaa gctcgcgatc gacaaggtgg
     4201 gcgtcaaggc gatccgccat ccgatgaaga tccaggagcg cgccggcggc gtgcagcaca
     4261 ccgtggcgac tttcaacatg tacgtatgcc tgccgcatca cttcaaaggc acgcacatgt
     4321 cgcgctttgt ccagatcctc gaggagcacg agcgcgagat cacggtcgac tcgttccagg
     4381 cgatgctgcg cgaaatggtg gaaaagctcg aggccgagga aggccacatc gagatgtcgt
     4441 tcccgtactt catcgagaag aaggcgccgg tgagcggggt cagaagcctg ctcgactacg
     4501 aagtgacttt cctcggcgag taccgcaaga agaatggccg cttcaccatg aaagtcgtgg
     4561 tgccggtgac gagcctctgc ccgtgctcga agaagatctc ggagcgcggc gcgcacaacc
     4621 agcgctcgca tgtcacggtc acggcgcgca cgcacgagtt cgtgtggatc gaggagatcg
     4681 tcgacatcgt cgaaagacag gcatcgagcg agctctacgg cctgctcaag cgtcccgacg
     4741 agaagttcgt caccgagcgc gcatacgaca acccgaagtt cgtcgaggac atggtgcgcg
     4801 acgtggccgc ggtgctgaat ctcgatgagc gcatcgacgc ctacgtggtc gagtcggaga
     4861 acttcgagtc gattcacaac cacagcgcct atgcgctgat cgagaggaac aaacgcgggc
     4921 gctagccctc gtcgcggcgc ttatgctcgg cttctccgcg gacgcgcagg accttcccgc
     4981 gcagcgcgcg cgcatggttt ccgaggtcca ggcgatgtat gcggagacgc agcgcgagac
     5041 cggcttcgcg gcgatgtcga acgaagtggc gcgcgccctc ggcaaggttg agcgccatcg
     5101 gctggtgccg ccgggcgagg cctcgcgcgc ttaccgcaat catccgctgc cgatcggcag
     5161 cggacagacc atctcccagc cctacatcgt ggccctctcg acggacctgc tgcgccccaa
     5221 gccgaccgac accgtcctcg aagtcggcac cggctcgggg taccaggccg cagtgctggc
     5281 cgaaatcgtc tcgacggttt attcgatcga ggtgatcccc gcgctcggca acgaggctcg
     5341 ccggcgcttg gccgagctcg gctaccgcaa tatcgaagtg cgcattggcg acggatacgc
     5401 gggctggccc gagaaagcgc cgttcgacgg catcgtggtg accgcggcgg cgccgcgcgt
     5461 gccgaaggcg ctcgtcgatc agctgaagcc cggcggccgc atggtcatcc cggtgggcgg
     5521 cgaaatggag attcagtacc tcaacgtgct gacgaagcgc gccgacggcg gcgtggacga
     5581 caagcgcgtc ctgccggtgc gcttcgtgcc tctggtgccg gggaagtgac taaaagcggc
     5641 gcgtaagggt catgaggtgc ccctcgcgcc tcatctccac tcgattcagg aagctcatgc
     5701 cgaggagcgc gacgtcgagg ccgcgctcga tgaccgtggc ttcgacgctg ttcagctcga
     5761 tgccgccgag ccggacggtg tcgagcgtga cgacgaatac cggcaccacc ccggcggcgg
     5821 tgtgggtgaa cccggatctg cccttgcgat agtcgaggcc caggcgcacc gcatcggcag
     5881 ccggcaacgc tacggtggtc gcgccggtgt caacgagaaa gcgcaccgaa ttgccgttca
     5941 ccgcgccctc ggcgaaaaaa tgcccgcgcg ggtcggcggc gacggtgatg ctctggcggt
     6001 cgctgcgcgg atcgaccgag cggtagtgct gtccgatggt caacgtgcgg cgcttgccgt
     6061 cgacttcgac gatggcctgg ccgcgttcga cggtgatgac cgtgatgccg ttccacttct
     6121 gtccgacacg cacggtcttc ggatcgcccc cgtccacggc cagcaccgca gccttgtcgc
     6181 cgatcaagcc gacgagcgcg acatcgtccc gcgccgcgca ggtgaaggcg aaggcgaatg
     6241 caaggactag ggcaataata cgggccatga gaccggttgc tatttttagg gccgccccga
     6301 ccgaaggtcc agggtatttt gccgcttatc tcgagcgtgc ctcaatcccg tggaagctcg
     6361 tcgcgctcga tgccggcgag gcagtgccgc gcgacccgcg cgaattttct ggcctcggca
     6421 tcatgggtgg cccgatgagc gccaacgacg acctgccgtg gattcctcca cttctcgagt
     6481 tcctgcgcga tgccgtgcgc cgtgacgttc caatcatcgg acactgcctc ggcgggcagc
     6541 tcatgtcgcg tgcgttcggc ggcgaagtgc gcgtaaaccc ggtgcgcgaa atcggctggg
     6601 gcgaagtgcg cgtcgccgac aacggcgtgg cgcgcgagtg gctcggcgat ctgcaatcgt
     6661 tccagacgtt ccagtggcac ggcgatacct tcagcatccc gcccggcgcg acgcgcgtgc
     6721 tcgaaggcga gcactgcgcg aaccaggcgt ttgccatcgg tcgccacctc ggcatgcaat
     6781 gccacgtcga aatgacgccc gagctcgtcc gcacctggct ctcgaccgga cagaaggaga
     6841 tcgtcgaaca tggcaagagt ccggcggtgc agtcgccggc cgagatcgag aaggacctcg
     6901 acacgcgcct cgagcgcctg cacgagatcg ccgaccggat ctacgaccgc tggaccgaaa
     6961 acctcagtag ggggtaaacg ggggagatta ccctccgttt caatcccgga agttgatgta
     7021 ttgcaggggg aggtcggtca ccgacttgcg caccagctga atggccgcct gaaggtcgtc
     7081 tttctttccg cctgagacgc gcactgcatc gccctggatc gaggcggcga ccttgatctt
     7141 cgaatccttg atgagcttct gaatgtgctt cgccttctcc tgcggcacgc cgaccttgac
     7201 cgtgaccggg cgcttcacct tgtcgccgga gatcttctcg atcgtgcccg actcgagcgc
     7261 gcgcacgtcg acgttgcgct tggcaagccg ctggcgcagc acgtcgagca cctggccgag
     7321 cttgaactcg tcatcggcgt agacggttag cgcgagctcg ttctgctcga ccctggcgtc
     7381 ggagcccttg aaatcgaagc ggttcgtgat ttccttgttc gtctgctcga ccgcgttctt
     7441 cacttcctgg cggtcgacct gcgacacgat gtcgaacgat ggcatcaggg cccttctagc
     7501 gagcgttgag ccgcttgagg tcgtcgatcc agcgattggt ggtcgagcgg atccattcca
     7561 ggtcggccgg gtcgtcgtac tgggtgtaca ccgaaagcgt gagcgccctg ccgcggagca
     7621 ggacgaggct gttggaggtg aggaggtagc gcgtgcgctc gaacatgccc gctttgctgc
     7681 gtgagccttg cagcaccgaa acggcgtcgg cttccttgcg caactccgcc agcatgacca
     7741 cggtgcccgg cgcgcgcgag tcgaggtgtt tctggaagtc gcccgtcggc aggtcggtga
     7801 cctcgccgcg cacgttacca gcgagcgatt tgaaagcgcc ttcgctcagg cgctctcgtt
     7861 ccatcgcgcg cggcgtgacc gcgatcatgt agcggcgcag ctcgagctga tcgccgagca
     7921 tgaagcgccg cagatcggcg tcgctcaggg cgaaaagcag cacgcggttc gaagccgagg
     7981 tgagcgactc gccgatctcc tgcagtcgcg gcgagccggt gaactgcgta tcggcgaatc
     8041 ccggcggcgc gtcgagcgcg actcgcgcat cgccgagctg cacggcgaac ggcgctgcga
     8101 gcgcgctgct cgcagcaaga agcatcgaga cgaggatgct gcgcatcgca tcccattgta
     8161 gccgctcgcg ccgaatcact tatgattagg cgcttggcga agatactcgg cagcctgccg
     8221 cagggcgaga agatcggcat tgcgttttcc ggcggcctcg acacgagcgc cgcggtgcac
     8281 tggatgcgcg cacatggcgc gattccccac gcgtacaccg cgaacctcgg ccagccggat
     8341 gaggacgatt acgacgcaat tccgaagaag gcgctcgcct atggcgcgga aaaggcgcgc
     8401 ctcgtcgagt gccggccgca gctcgtcgcc gagggtatcg ctgccatcca gtgcggcgcc
     8461 ttccacatct ctaccggagg cgccacctac ttcaacacca cgccgctcgg ccgtgcggtc
     8521 acggggacga tgctcgtcct cgccatgcgc gaggacgaag tcggcatctg gagcgacggc
     8581 agtacctaca agggcaacga catcgagcgc ttctaccgct acgggctgct cgccaacccg
     8641 gcgctgcgca tctacaagcc gtggctcgac cagcgcttca tcgacgagct cggtggccgc
     8701 aaggagatgt cggcgtacct cgccaaggcc ggcttcgact acaagacgag cgccgagaag
     8761 gcgtattcga ccgattccaa catcctgggc gcgacgcacg aagcgaagga tctcgagttc
     8821 ctggataagg gcatcgccat cgtcgagccc atcatgggcg tggcgttctg gcgcgaggac
     8881 ctaagaatca agaatgaaaa agtaactgtg gaaatcgccg aaggtgcgcc cgtggcgctc
     8941 aacggccagc gcttctccga tccggtcaag ctcatggagg aagccaatgc catcgggggt
     9001 cgccacggct tgggcatgtc ggaccagatc gagaaccgca tcatcgaggc gaagagccgg
     9061 gggatctacg aggcgccggg aatggcgctg ctgcacatcg cctacgagcg cctcgtcacg
     9121 gg
//
LOCUS       DASTPD010000295         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1181260, whole genome shotgun sequence.
ACCESSION   DASTPD010000295 DASTPD010000000
VERSION     DASTPD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181260"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFR57_09790"
     CDS             complement(<1..462)
                     /locus_tag="VFR57_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259695.1"
                     /translation="MTLVAVRDLRVHFDTVHAVRGVSFDVEEEATLALVGESGSGKSV
                     TALSILGLLPPAARAAGEIVYGGRDLLTIPPREMRALRGAGISMIFQEPMSSLNPVFS
                     VGFQIAEALGEHLGMARAERRRRTLELLHEVRLSNPARAIDACPFQLSGGEQ"
     gene            complement(459..1451)
                     /locus_tag="VFR57_09795"
     CDS             complement(459..1451)
                     /locus_tag="VFR57_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259696.1"
                     /translation="MNLWRLGWQRLLHDRVGAASAAVVLAFLLIAAASAAGLIARDWA
                     AEVGVSYAPPAFLGSAVSEQRTQARAAHAPREDHGIVDPLAEELAEIRSTLAGYAGSE
                     AKAQTLFFGGDKWGRDVIKKVIKGTETSLVVGLCAAALAVFLGTLCGALAGYHGGWLD
                     DFFNWLYSVFTSIPYLLLVLAIAAVLDQKGIVTVILILGLTGWTGIFRLIRAEYLKHK
                     VREYVLAADAIGASHARRMFAHILPNVGHVVLVQLSLHVVLFIKSEVILSFLGFGVGV
                     DTVSWGSMLAEAPSELILGMWWQIAVATVAMALAVTAFSLLTDAVRDALDPRLQ"
     gene            complement(1448..2386)
                     /locus_tag="VFR57_09800"
     CDS             complement(1448..2386)
                     /locus_tag="VFR57_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259697.1"
                     /translation="MLSYLAKRLAQFVPTLLGVMALVFFVFNWVGGDPAYILAGKIAS
                     PERIEEIRRGLGVDQPYWVQLGIFVKQIALADFGASWSTNERVSQIFATRLGPSLTVL
                     IPLLAISTVLSLAAAMLVAYYRGSLTDRAVMIACTVGQSISILVYILVFQYVLAYRLG
                     WFPVQGWGGTLAENLLAYAALPILVGVVVSLAPDIRLYRSFFLEEIHQDYVRTARAKG
                     LGEGRVMWLHVLRNASIPLITNVMAQIPGLLAGAFLIERFFSIPGIGREVILAVERSD
                     FPVIKAVTVYVAVATLLINLAADLVYRALDPRVQLK"
     gene            complement(2367..4001)
                     /locus_tag="VFR57_09805"
     CDS             complement(2367..4001)
                     /locus_tag="VFR57_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259698.1"
                     /translation="MRLLALVLGLSVAGAQAADPAKILRITFQAAETGFDPVKVSDYY
                     SGTVISAIFDPLLTYDYLARPAKLVPNTAVALPDIADGGKTYTVKVRPGIYFADDPVF
                     KSARRELTALDYAYSIRRFLEPKNHSPYAFLFEGIAGIETPERHTLVIRLKQPDYNFS
                     HVLAFALAGAVAREAIESYGDESAARPVGTGPFRLKRYMRSSKIVLEANPGFRELVYE
                     GQRLPRIGGVEISIMEETQSRWLAFQRGDTDLEYQLAEVAPTFITAEGKLKPELARKG
                     IRLERTIDPEIIYLYFNMQDKTVGGLSKERVALRRAIAMAYKIEDQIRIIRKSQSIRT
                     HYPIPPGVAGHDPAYRSSIEHDPRAANALLDRFGYRKGPDGYRRMPNGEALAIRYSST
                     PSERDRQFDELMKRSLDSIAVRLEIHKDRFPELIKLENQCRLMMRSAAWIADYPDGDN
                     FMQLLYGPNSGQSNNACYRSAEYDRRYEKSRLLPDGPERDRLYREMARIMEVDTVWLL
                     TDSRYRNVLMQPHVKGFTKHPVLHAEWLYIDLELPR"
     gene            4025..>4410
                     /locus_tag="VFR57_09810"
     CDS             4025..>4410
                     /locus_tag="VFR57_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0259699.1"
                     /translation="MGVLSGKKILITGVLSSRSIAYGVAKAARREGAELAFTYQNDRF
                     KDRVTDMAKELGSEIALPCEVTSDQEIQQLFSRLGEKWQDLDGLVHAIAFAPREAIAG
                     EFLDGITREAFRQAHDISAYSFPALA"
BASE COUNT          813 a         1377 c         1492 g          728 t
ORIGIN      
        1 ctgctcgccg cccgagagct gaaaaggaca ggcgtcgatc gcccgggccg ggttcgacag
       61 gcgaacttcg tggagcagct cgagcgtgcg gcggcgtctt tccgcgcgcg ccattccgag
      121 atgctcgccg agggcctcgg cgatctggaa gccgacgctg aacaccgggt tgagcgagct
      181 catcggctcc tggaagatca tcgagatccc ggcaccgcgc agcgcgcgca tttcccgggg
      241 cgggatcgtc aggaggtctc tgccgccgta gacgatttcg cccgccgcgc gagccgccgg
      301 cggcagcaag ccgaggatcg aaagcgcggt gacggacttg ccgctgcccg actcgccgac
      361 aagcgcgagg gtcgcctctt cctcgacgtc gaagctgacg ccccgcactg cgtgcaccgt
      421 gtcgaaatgc acgcgcagat cgcgcaccgc gacgagcgtc attgcagcct cgggtcgagc
      481 gcgtcgcgca ccgcgtcggt caagaggctg aacgcagtca ccgccagcgc catggcgacg
      541 gtcgcgacgg cgatttgcca ccacatgccg aggatcagct cgctcggcgc ttcggcgagc
      601 atgcttcccc aggacacggt atcgacgccg acgccgaagc ccaggaagct caggatcacc
      661 tccgacttga tgaagagcac gacatgcagc gaaagctgta ccagcaccac atggccgacg
      721 ttcggcagga tatgcgcgaa catgcgccgc gcatgagagg cgccgatcgc gtcggcagcc
      781 agcacgtact cgcgcacttt gtgcttgaga tactcggcgc ggatcaaccg gaagatcccg
      841 gtccagcctg tgaggccaag gatcaggatg accgtgacga tgcccttctg gtcgagtacc
      901 gcagcgatcg cgagcaccag cagcaggtac ggaatcgagg tgaagacgct gtagagccag
      961 ttaaagaaat cgtccagcca cccgccgtgg tagcccgcga gcgcgccgca cagtgtgccg
     1021 agaaacacgg cgagcgccgc ggcgcagagg ccgacgacga gcgaggtttc ggtacccttg
     1081 atcactttct tgatcacgtc tcgcccccac ttgtcgccgc caaaaaacaa ggtttgcgct
     1141 ttcgcttcgc tcccggcgta cccggccagg gtgcttctta tttcggccag ctcttcggcc
     1201 agggggtcga caatgccatg gtcttcgcgt ggagcgtgcg ccgctcttgc ttgagtccgt
     1261 tgctcgctga ccgcagaacc aaggaaagcc ggcggtgcgt agctgacgcc gacctctgcg
     1321 gcccagtcgc gggcaatcag gcctgcggcg gaggctgctg cgatcagcag gaaggcaagg
     1381 accacggctg cggaggcggc cccgacgcgg tcgtgcagca aacgttgcca gccgaggcgc
     1441 cacaggttca tttgagctgc acccgcggat cgagcgcgcg gtaaaccagg tccgcggcaa
     1501 ggttgatgag cagcgtggcg acggcgacgt acaccgtgac cgccttgatc acggggaagt
     1561 cgctgcgttc caccgcgagg ataacctcgc ggccgatgcc ggggatggag aagaagcgct
     1621 cgatgaggaa cgcgccggcg agcagtcccg ggatctgcgc catcacgttg gtgatgagcg
     1681 ggatcgaagc gttgcgcagg acgtgcagcc acatgacccg accctcgccc aagcccttcg
     1741 cacgcgcggt gcgcacgtag tcctggtgga tctcttcgag gaagaagctg cgatagagac
     1801 ggatgtcggg cgcaagcgag accaccacgc cgacgaggat cggcagcgcg gcgtaggcaa
     1861 ggagattttc cgcgagcgtc ccgccccagc cctgcaccgg aaaccagccg agccggtagg
     1921 caagcacgta ttggaaaacg aggatgtaga cgaggatgct gatcgactgg ccgacggtgc
     1981 acgcgatcat caccgcgcgg tcggtgagcg agccgcggta gtacgcgacg agcatggcgg
     2041 cggccaggga aaggacagta ctgatcgcca agagcgggat gagcacggtg agcgaaggcc
     2101 caaggcgcgt cgcgaagatc tgcgacacgc gctcgttggt ggaccagctt gcgccgaaat
     2161 cggccagcgc aatctgtttg acgaagatcc cgagctgtac ccagtacggc tggtcgacgc
     2221 cgaggccgcg ccggatctcc tcgatgcgct cggggctcgc gatcttgccg gcgagaatat
     2281 acgccgggtc gccgccgacc cagttgaata cgaaaaacac gagcgccatc acgccgagca
     2341 gcgtgggaac gaactgcgcg agccgcttag cgaggtagct caagatcgat gtacagccat
     2401 tccgcgtgca gcacgggatg cttggtgaag cctttcacat gcggctgcat gagcacgttg
     2461 cggtagcggc tgtcggtgag aagccagact gtgtccactt ccatgatgcg cgccatctcc
     2521 cggtacagcc ggtcgcgctc cgggccgtcg ggcagaaggc gggacttctc gtatcgccgg
     2581 tcgtactcgg cggagcggta gcatgcgttg ttactctgcc cgctgttcgg gccgtagagg
     2641 agctgcatga agttgtcgcc gtccgggtag tcggcgatcc aggcagcgct gcgcatcatc
     2701 aggcggcact ggttctcgag cttgatgagc tccgggaagc ggtccttgtg gatttccagg
     2761 cgcacggcga tcgagtcgag agagcgcttc atcagctcgt cgaactgccg gtcgcgctcg
     2821 ctcggggtcg aggagtagcg gatggcgagc gcctcgccgt tcggcatgcg ccggtagcca
     2881 tccgggccct tgcggtagcc gaacctgtcg agcagcgcgt tcgccgcgcg cggatcgtgc
     2941 tcgatgctcg agcggtaggc gggatcgtgg cccgcgacgc cgggcgggat cgggtagtgc
     3001 gtgcggatcg actggctctt gcggatgatg cggatctgat cctcgatctt gtacgccatc
     3061 gcgatcgcgc gccgcagggc gactcgctcc ttggaaagcc cgccgacggt cttgtcctgc
     3121 atgttgaagt agaggtagat gatctccggg tcgatggtgc gctcaagccg gatgcccttg
     3181 cgcgcgagct ccggtttgag cttgccctcg gcggtgatga aggtcggcgc cacctccgcg
     3241 agctggtact cgaggtcggt atcgccgcgc tggaacgcga gccagcggct ctgcgtctcc
     3301 tccatgatgc tgatctcgac cccgccgatg cgcggcaggc gctgtccctc gtacacgagc
     3361 tcgcgaaaac caggattcgc ctcgagcacg atcttcgagg agcgcatgta gcgcttcagg
     3421 cgaaacggtc ccgtgccgac cgggcgcgcc gccgattcgt cgccgtagga ctcgatggcc
     3481 tcgcgcgcca ctgcgccagc gagcgcaaac gcgagtacgt gcgagaagtt gtagtcgggc
     3541 tgcttcagac ggatgacgag cgtgtgacgc tcgggcgtct cgatgccggc gatgccttcg
     3601 aagaggaagg cgtagggaga gtggttcttc ggctcgagga accgccgaat cgagtatgcg
     3661 taatcgagcg ccgtcagctc gcgtcttgcg ctcttgaaca ccgggtcgtc ggcaaaatag
     3721 attccgggcc ttactttgac ggtataggtc ttcccaccgt cggcgatgtc ggggagggcc
     3781 acggcggtat tcggcacaag cttcgccggc cgcgcgaggt agtcgtaggt gagcaatggg
     3841 tcgaatatgg ccgagatcac ggtgcccgag tagtagtcgg acaccttcac cggatcgaaa
     3901 ccggtttccg ccgcctggaa cgtgatgcgc agaatcttcg cgggatcagc cgcctgcgcg
     3961 cccgcgaccg acagaccgag cactagagcg aggagtctca agcgcggaaa gtataatcgg
     4021 gcgcatgggc gtgctttccg gcaagaagat cctcattact ggcgtccttt ccagccgctc
     4081 gatcgcgtac ggcgtcgcca aagccgcgcg ccgcgaaggc gccgagctcg cgttcacata
     4141 ccagaacgat cgcttcaagg accgcgtcac cgacatggca aaggagctcg gctccgaaat
     4201 cgcgctcccg tgcgaggtga ccagcgatca ggaaattcag cagctctttt cccgtctggg
     4261 ggaaaaatgg caagacctcg atggtctggt gcatgcgatc gcgttcgcgc cgcgcgaggc
     4321 gatcgcgggc gagtttctcg acgggatcac ccgcgaggcg ttccggcagg cgcacgacat
     4381 ctcggcctac agcttccccg cgctcgccaa
//
LOCUS       DASTPD010000296        12381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_156907, whole genome shotgun sequence.
ACCESSION   DASTPD010000296 DASTPD010000000
VERSION     DASTPD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156907"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFR57_09815"
     CDS             complement(<1..446)
                     /locus_tag="VFR57_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259700.1"
                     /translation="MRKLLIAAATGSMLALGAPAHAQQKVRINLANAFPNNLTLIGEA
                     PGKLAQKLNRLSAGQIELRVNEPGALVPALQAIQATGQGSVDAAWSNAGFFAATDTAF
                     NMFASVPFGPAIPEYLAWIHHGGGLQLSREMFAKHGVYNIPCAIIPP"
     gene            502..1206
                     /gene="tpiA"
                     /locus_tag="VFR57_09820"
     CDS             502..1206
                     /gene="tpiA"
                     /locus_tag="VFR57_09820"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0259701.1"
                     /translation="MARVRLVAGNWKMHGSRTSVAALLDELVKANPPGCAVCAPFPYL
                     AQVAERLRGTRIAWGAQNVSEHAQGAYTGEVSAAMLADFGCRYVIVGHSERRHIFGES
                     DAQVAAKFAAAAKAGLTPILCVGETLEERDAGKTEAVVQRQLSAVDFKGGVLAYEPVW
                     AIGTGRNATPEQAQAVHGFLRKRVPADTPILYGGSVKPQNAAAIFAMPDVDGGLIGGA
                     SLVAKDFLDIVKAAAK"
     gene            1203..1574
                     /gene="secG"
                     /locus_tag="VFR57_09825"
     CDS             1203..1574
                     /gene="secG"
                     /locus_tag="VFR57_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0259702.1"
                     /translation="MTGIWLNILIIVHVLVALVIIGLVLLQHGKGADMGSGFGGGASG
                     SLFGATGSANFLSRTTAVLAAIFFVLSLALAYVATKKPVQSGGGVIDAVRSRPVEEKK
                     AAPPAPEAPKPPAPQEKQVPQ"
     gene            1604..1691
                     /locus_tag="VFR57_09830"
     tRNA            1604..1691
                     /locus_tag="VFR57_09830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Leu,seq:gag)
     gene            1695..2054
                     /locus_tag="VFR57_09835"
     CDS             1695..2054
                     /locus_tag="VFR57_09835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0259703.1"
                     /translation="MMLAEYFPILLFILVGLAVGVVPMLLGKLLGPNRPDPEKLSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWASVINDIGFPGFIAMMVFLAI
                     LTVGFVYEWMKGALEWE"
     gene            2045..2521
                     /locus_tag="VFR57_09840"
     CDS             2045..2521
                     /locus_tag="VFR57_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU0259704.1"
                     /translation="MGIEGVLQQGFVTTTVDKVVNWTRTGSLWPVTFGLACCAIEMMH
                     AGAARYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVVSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLVQLQNKIKRTNTIAR"
     gene            2618..2833
                     /locus_tag="VFR57_09845"
                     /pseudo
     CDS             2618..2833
                     /locus_tag="VFR57_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2833..3309
                     /gene="nuoE"
                     /locus_tag="VFR57_09850"
     CDS             2833..3309
                     /gene="nuoE"
                     /locus_tag="VFR57_09850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEU0259705.1"
                     /translation="MLTTDSLKKIDREVAKYPDDQKQSAVMSALIIAQDEKGWLSTET
                     MDYVAKYLGMPPVQVYEVATFYSMYNLKPTGQYKLTLCTCLPCGLQGALDAGDHLREK
                     LGIDFNETTPDGKFTLKEGECMGACAMAPVLLVNNKKMLDYMTNEKLDQLIETLRK"
     gene            3309..4661
                     /gene="nuoF"
                     /locus_tag="VFR57_09855"
     CDS             3309..4661
                     /gene="nuoF"
                     /locus_tag="VFR57_09855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0259706.1"
                     /translation="MLDYGPDAILMKGLDGANWRLKDYEARGGYHALRKILADRLSPD
                     QVVAEVKKSALRGRGGAGFPTGLKWSFMPKQFTGPKYVVCNSDEGEPGTCKDRDLLRY
                     DPHSVIEGMVIAGYAMGASAGYNYIHGEVWEIYEQFEQALEEARAAGFIGKSILGSDF
                     SFELHAVPGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFA
                     AVPWIMRHGGEAFLALGRPNNGGTKLFSVTGHVNRPGNYEVKLGTPFAKLLEMAGGMR
                     GGRKLKGVIPGGSSMPVLPADIMMATDMDYDSIAKAGSMLGSGAVIVMDETTCMVRAL
                     ERLSYFYFEESCGQCTPCREGTGWLYRVVNRIERGLGRMEDLDLLVNVSDNIAGRTIC
                     ALGDAAALPVKSFIQHFRDEFVFHIEKKACLHGTVEPKWGRAGARHEPHVVRNMAEAA
                     "
     gene            4661..6832
                     /gene="nuoG"
                     /locus_tag="VFR57_09860"
     CDS             4661..6832
                     /gene="nuoG"
                     /locus_tag="VFR57_09860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU0259707.1"
                     /translation="MPKVFVDGTEVEVAQGATVMDATNKVGVYVPHFCYHRKLTIAAN
                     CRMCLVEIEKAPKPMPACATPVAEGMKVWTHSKVAKDAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSNSRYTEEKRVVLRKELGPLVAAEEMSRCIQCTRCVRFGQEI
                     AGIMELGLAGRGENAEIVSFVGRAVESELSGNMIDLCPVGALTSEPFRYKARTWELSR
                     RRSISPHDSLGSNLVVQVRQNTVMRVLPLENDAVNECWLSDKDRFSYEGLNSPDRLQR
                     PLVKRDGNWQELDWEQAFEVLLPKLQGEFGVLASPHATLEELFLVSRLGAPADFRLRQ
                     SDFSFRAAPWLGMPIAELDKLDRVLVVGSFLRKDHPLIAHRLRQAAKRGAQIHMLHSV
                     DDDWLMPIAGKKIVAPADLPSALGSYGELFAGSKNPAVFLGNFAMQHPRAGEILAAAN
                     ALGVKVGVFGEAANSVGGTLAGLPSAGNVNDVLGKKSIVLLNVEPELDCADPVAARKA
                     LDAAEFVVSLTPWKTGLEYADVLLPITPFTESSGTFVNCEGRVQSYHATVNALGNARP
                     GWKVLRVLGSLLGREGFELDTIEQVRAACLDGKDIPKLLSNSVQAAGSARASVSGIQR
                     IADVPIYFADPLVRRAPSLQKTKDARPPRAWMNSKLLAKLGAAAGQPVLVNGSVKLMA
                     ALDDRLPDECVRIAAGHPTTAAVGPMFGMLELARLATEKAA"
     gene            6833..7909
                     /gene="nuoH"
                     /locus_tag="VFR57_09865"
     CDS             6833..7909
                     /gene="nuoH"
                     /locus_tag="VFR57_09865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU0259708.1"
                     /translation="MEWLAERSTSWFGPVWGPVFYETGTNLAFIVAIVVPLMLAVAYL
                     TLWERKLIGWIQIRIGPNRVGPFGLLQPIADAIKLLMKELILPTNANKGLYLLAPVLM
                     IMPALAAWAVIPFSPELVLADINAGLLYIMALSSLGVYGVIVAGWASNSKYAFLGAMR
                     SAAQMVSYELAMGFALVVVLMVSGSLNLSDIVKGQGEGYFASRGVTFMSWNWLPLLPM
                     LIVYFVSGVAETNRAPFDVVEGESEIVAGHMIEYSGMSFALFFLAEYMNMLLISALTA
                     VMFFGGWQAPIDAPVLHLIPPFGWLLVKIFLIVSMFLWIRATFPRYRYDQIMRLGWKV
                     FIPLTLVWIAVIAIWMQTPWNIWP"
     gene            7906..8394
                     /gene="nuoI"
                     /locus_tag="VFR57_09870"
     CDS             7906..8394
                     /gene="nuoI"
                     /locus_tag="VFR57_09870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0259709.1"
                     /translation="MTRVLDFFKSFLLLELLRGMALTGRHMFARKITVQFPEEKTPLS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAIHIESEQRADGTRRTTRYDVDLVKC
                     IFCGFCEEACPVDAIVETRTLEYHGEKRGDLYMTKPMLLAVGDRYEEQIARDRAQDAR
                     YR"
     gene            8406..8999
                     /locus_tag="VFR57_09875"
     CDS             8406..8999
                     /locus_tag="VFR57_09875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU0259710.1"
                     /translation="MMFETWLFYAFAAVLLVSGLLVITARNPVHGVLWLVLAFFTAAG
                     IWLLLRAEFLAITLVVVYVGAVMVLFLFVVMMLDINIERVREGFWRNLPLALVVGGVM
                     VLEMVAVLANRYHGAVPRDLPAGYSNTKALGRVLYTQYAFAFEIAAVLLLVAIIAAIA
                     LTLRRRKDSRYQDPSRQVKARREERVQLVSMKPEVDK"
     gene            8996..9310
                     /gene="nuoK"
                     /locus_tag="VFR57_09880"
     CDS             8996..9310
                     /gene="nuoK"
                     /locus_tag="VFR57_09880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU0259711.1"
                     /translation="MSGPLSLSHFLVLGAILFAISVVGIFLNRRNLIVLLMAIELMLL
                     AVNMNFIAFSYFLGDLAGQVFVFFILTVAAAESAIGLAILVVLFRNLNTIDVEDLDAL
                     KG"
     gene            9337..11211
                     /gene="nuoL"
                     /locus_tag="VFR57_09885"
     CDS             9337..11211
                     /gene="nuoL"
                     /locus_tag="VFR57_09885"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU0259712.1"
                     /translation="MKALYLIVPFAPLVAAIVAGVFGKQVGRAGAHSVTIFAVLLSFL
                     CSCVVFYDVLQGTRFNGPVYTWMTSGDAAFQIGFLIDPLTTLMMVVVTFVSLMVHIYT
                     IGYMHDDPGYQRFFSYISLFTFAMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTRE
                     TAVYANLKAFLVNRVGDFGFLLGIALILAQYGTLDYAEVFEYAQAVPGEITLICVLLF
                     IGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLYELSETARS
                     VVLVIGAVTAFSMALVALTQYDIKRVVAYSTLSQLGYMTAALGASAYSAAMFHLMTHA
                     FFKAVLFLAAGSVIIALHHEQDMRRMGGLKKYMPFTYWTMLMGAISSAGIPGFAGFFS
                     KDAIIEAVHHSATPGATFAYVLVLVTVFVTAAYTFRLVFMSFHGPERFDKHHPPHESP
                     AVVTVPLVLLAIPSVAAGWVIGDVVFGNYFAESLPAPDPNYHGLYSFITHGLVSVPFW
                     LALAGIATAWLLYMKKPQLPKRLAMELGPAYSIVERKYGYDELYSWLFAGGARALGKG
                     FWRAGDQAVIDGLVVNGSARVVGWFSGVVRLLQSGFIYRYASVMLFGVVILAFWFLKN
                     "
     gene            11212..>12381
                     /locus_tag="VFR57_09890"
     CDS             11212..>12381
                     /locus_tag="VFR57_09890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU0259713.1"
                     /translation="MLLSLAIWIPILAGLAVFALGGDERAPAQRWLALAGAVLGFLVT
                     IPLYTGFDASNAGFQFEEKKLWIETFNIHYHLGVDGISVLFVLLNSFITILVVLAGWS
                     VIDNKVGQYYGAFLIMSGLLNGVFAALDAVLFYVFFEGTLIPMFIIIGVWGGPNRVYA
                     AVKFFLYTLLGSLLMLVAMLYLYNKAGGSFSITDWHHVPLPLGVQIVLFFGFLLAFAV
                     KVPMWPVHTWLPDAHTEAPTGGSVVLAAILLKLGAYGFVRFTLPVLPDASRELAWLMI
                     ALSLVAIAYISLVTLVQTDMKRLIAYSSVAHMGFCTLGFFLFNAYGVEGALVQMISHG
                     FVSAALFLCIGVMYDRMHSRNIADYGGVVNTMPKFAALMMLFAMANCGLPATSGFV"
BASE COUNT         1992 a         4066 c         3979 g         2344 t
ORIGIN      
        1 ggcgggatga tggcgcacgg gatgttgtag acgccgtgct tcgcgaacat ctcgcgcgaa
       61 agctgcagcc cgccgccgtg atggatccac gcgaggtatt cgggaatggc cggcccgaac
      121 ggcaccgagg cgaacatgtt gaaggcggta tcggtcgcgg cgaaaaaacc ggcgttcgac
      181 cacgcggcgt ccaccgagcc ttggccggtc gcctggatcg cctggagtgc gggcacgagc
      241 gcgccgggct cgttgacgcg cagctcgatc tgtccggcgg acaggcgatt gagtttctgc
      301 gcgagcttgc cgggcgcttc gccgatcagc gtaaggttgt tcggaaatgc attcgcgaga
      361 ttgatgcgca ccttctgctg cgcgtgcgcg ggcgcgccga gcgcgagcat gctgcccgtc
      421 gccgcggcga ttagaagttt cctcattgct ctccctctgg atttaaaact gcgcggggat
      481 attacggtaa aattcagggg catggcgcgc gttcggctgg tggccggcaa ctggaaaatg
      541 cacggcagcc gaacctcagt cgcggcgctg cttgatgaac tcgtcaaagc aaatccgccg
      601 ggctgcgcgg tgtgcgcgcc gtttccgtat ctcgcgcagg tcgcggagcg gctgcgcggt
      661 acgcgcatcg cctggggcgc gcagaacgtg tctgagcacg cgcagggcgc ttatacgggc
      721 gaggtttcgg ccgccatgct cgcggacttc gggtgccgct acgtcatcgt cgggcattcc
      781 gagcgccggc acatcttcgg cgaaagcgac gcccaggtag cggcaaagtt cgccgccgcg
      841 gcgaaagcgg gcctgacgcc aatcttgtgc gtgggcgaga cgctcgaaga gcgtgacgcc
      901 ggaaagacgg aggcggtggt gcaaaggcag ctttccgcgg tggatttcaa gggcggcgtc
      961 ctcgcctatg agccggtctg ggcgatcggc accgggcgta acgcaacgcc cgagcaggcg
     1021 caggcggtgc acgggttcct gcgcaagcgg gtgcccgcag ataccccgat cctttatggg
     1081 ggcagcgtca agccgcagaa cgcggcggcg attttcgcga tgcccgacgt cgacggcgga
     1141 ctgatcggcg gcgcatcgct cgtggcgaag gattttctgg atatcgtgaa ggcggcggcg
     1201 aaatgaccgg catctggctg aacatcctca tcatcgtgca cgtgctggtg gcgctcgtca
     1261 tcatcgggct cgtgctcctc cagcacggca agggcgccga catgggctcg ggcttcggcg
     1321 gcggcgcctc cgggagcctg ttcggcgcca cgggctcggc gaatttcctc tcgcgcacca
     1381 cggccgttct cgcggcgatc ttcttcgtgc tgagcctcgc gctcgcctac gtcgcgacga
     1441 agaagccggt ccaatccggc ggcggcgtta tcgacgcggt acgcagccgt cccgtcgagg
     1501 aaaagaaagc cgcgccgccc gcgcccgagg cgccgaagcc gccggcgccc caggagaagc
     1561 aggtcccgca gtgatatttt tgagcgataa tcgtgggcta agtgcccacg tggtgaaatt
     1621 ggtagacacg ctagcttgag gggctagtgg cctaaaaagc cgtagcagtt cgagtctgct
     1681 cgtgggcacc accaatcatg ctcgcagagt actttccgat cctgctattt attctggtag
     1741 gcctcgccgt cggcgtggtg ccgatgttgc tcggcaagct tctcggtccg aaccggccgg
     1801 atccggagaa gctctcgccc tatgagtgcg ggttcgaagc attcgaagac gcgcgcatga
     1861 aattcgacgt gcgttattac ctcgtcgcaa ttctcttcat tttgttcgac ctcgaaatcg
     1921 ccttcctctt cccctgggcc tcggtcatca acgacatcgg ctttccgggc ttcatcgcaa
     1981 tgatggtttt tcttgcaatt cttaccgtgg gcttcgtcta cgagtggatg aaaggcgcgc
     2041 tggaatggga atagaaggag tcttgcagca gggtttcgtc accaccacgg tggacaaggt
     2101 ggtgaactgg acgcgcaccg ggtcgctctg gccggtgacc ttcggcctcg cctgctgcgc
     2161 gatcgagatg atgcacgccg gcgcggcgcg ctacgacctc gaccgcttcg gcatcgtgtt
     2221 ccggccgagc ccgcgccagt ccgacgtcat gatcgttgcc ggcacgctct gcaacaagat
     2281 ggccccggcg ctgcgcaagg tctatgacca gatgccggag ccgcgctggg tggtctcgat
     2341 gggctcgtgc gcgaacggcg gcggctacta ccactactcg tactcggtgg tgcgcggctg
     2401 cgaccgcatc gtgccggtcg atatctacgt ccccggctgc ccgccgaccg cggaggcgct
     2461 cctctacggc ctcgtgcagc tgcagaacaa gatcaagcgc acgaatacga tcgcccgtta
     2521 gatgacacca cggctccagc gcctctccga ttcgatcaag gatctgaacc cggttgagcg
     2581 cctcggcgag ctgaccgtcg aggtgaaagc ggctgacgac cgctacctcg tgcgcgtgga
     2641 ggagatgcgc cagtcgaacc gcatcgtcaa gcagtgcatc gactggcggc gcaagaaccc
     2701 cgggccggtg atggtcgaca accataaggt cgcgccgccc gcgcgcggcg agatggcgcg
     2761 cggccacatg atcgccgacg cggtcgcgat tatcggcacg caggacatcg tcttcggcga
     2821 ggtcgaccgc tgatgttgac gacggactcg ctcaagaaga tcgaccgcga ggtcgcgaag
     2881 tacccggacg accagaagca gtcggcggtg atgagcgcgc tcatcatcgc gcaggacgag
     2941 aaggggtggc tgtcgaccga gacgatggac tacgtcgcca agtacctcgg catgccgccg
     3001 gtgcaggtgt acgaggtcgc caccttctac agcatgtaca acctcaagcc gacggggcaa
     3061 tacaagctca cgctgtgcac ttgcctgcca tgcggtttgc agggtgcgct ggacgcgggg
     3121 gaccacctgc gggagaagct cggcatcgat ttcaacgaga ccacgccgga cggcaagttc
     3181 acgctgaagg agggcgagtg catgggcgcg tgcgccatgg cgccggtgct cctcgtcaac
     3241 aacaagaaaa tgctcgacta catgacgaac gagaagctcg atcagctgat cgagaccctg
     3301 aggaaatgat gctcgattac ggacccgacg ccatcctcat gaagggcctc gacggcgcga
     3361 actggcgtct caaggattac gaggcgcgcg ggggctacca cgccctgcgc aagatcctcg
     3421 ccgacaggct ttcgcccgac caggtggttg ccgaggtgaa gaaatcggcg ctgcgcggcc
     3481 gcggcggcgc gggctttccg accgggctca agtggtcgtt catgccgaag caattcaccg
     3541 gtcccaagta cgtcgtgtgt aactccgacg aaggcgagcc cggcacgtgc aaggatcgcg
     3601 acctcctgcg ctacgacccg cactcggtga tcgagggaat ggtcatcgcc ggctacgcaa
     3661 tgggcgcgag cgccggctac aactacattc acggcgaagt ctgggagatc tacgagcagt
     3721 tcgagcaggc gctggaagag gcgcgcgcgg ccggcttcat cggaaagagc atcctcggct
     3781 ctgatttcag cttcgagctc cacgcggtgc cgggctacgg cgcatacatc tgcggcgagg
     3841 aaaccgcgct cctcgagtcg ctcgaaggaa aaaaaggcca gccgcgcttt aagccgcctt
     3901 tcccggcgag cttcggcctc tacggcaagc cgaccacgat caacaacacc gagaccttcg
     3961 ccgcggtgcc ctggatcatg cgccacggcg gcgaggcgtt cctcgcgcta ggcaggccga
     4021 ataacggcgg caccaagctc ttttcggtga ccgggcacgt gaaccggccc ggcaactacg
     4081 aagtgaagct cggcacgccg ttcgcgaagc tgctcgagat ggccggcgga atgcgcggcg
     4141 gccgcaagct gaaaggcgtg atccccggcg gctcgtcaat gccggttctg ccggccgaca
     4201 tcatgatggc gacggacatg gactacgact cgatcgccaa ggcgggctcc atgctcggct
     4261 ccggcgcagt catcgtcatg gacgagacca cctgcatggt gcgcgcgctc gagcgcctgt
     4321 cgtacttcta cttcgaggag tcctgcggcc agtgcacgcc gtgccgcgag ggtaccggct
     4381 ggctctaccg cgtggtgaac cgcatcgagc gcggcctcgg gcgcatggag gacctcgatc
     4441 tcctcgtcaa tgtttccgac aacattgccg gccgcaccat ctgcgcgctc ggcgacgcgg
     4501 cggcgcttcc ggtgaagagc ttcatccagc acttccgcga cgagtttgtt ttccacatcg
     4561 aaaagaaagc ctgtctgcat ggcacggtgg agcccaagtg gggccgcgcc ggtgcgcgcc
     4621 acgagccgca tgtcgtgcgt aacatggcgg aagccgcctg atgcctaaag tcttcgtcga
     4681 cggaacggaa gtcgaagtcg cgcaaggcgc gaccgtgatg gacgcgacca acaaggtcgg
     4741 cgtctacgtt ccgcacttct gctatcaccg caagctcacc atcgcggcga actgccgcat
     4801 gtgcctggtc gagatcgaga aggcgcccaa gcccatgccg gcgtgcgcga cgccggtcgc
     4861 cgaaggcatg aaagtgtgga cgcattccaa ggtggcgaag gacgcgcaga acggcgtcat
     4921 ggagttcctg ctcatcaacc atccgctcga ctgcccgatc tgcgaccagg gcggcgagtg
     4981 ccagctgcag gacctcgcgg tgggctacgg cggctccaat tcgcgctata ccgaagagaa
     5041 gcgcgtggtc ctgaggaagg agctcggccc gctggttgcg gccgaagaga tgagccgttg
     5101 catccagtgc acgcgctgcg tgcgcttcgg ccaggagatc gccgggatca tggagctcgg
     5161 cctcgccggg cgcggcgaga acgccgagat cgtctccttc gtcggccgcg cggtcgagtc
     5221 cgagctctcc ggcaacatga tcgatctctg cccggtgggc gcgctcacga gcgagccgtt
     5281 ccgctacaag gcgcgcacct gggagctctc gcgccggcgc tcgatctcgc cgcacgactc
     5341 gctcggctcg aacctcgtcg tgcaggtgag gcagaacacg gtgatgcgcg tgctcccgct
     5401 cgagaacgac gcggtcaacg agtgttggct ctcggacaag gaccgctttt cctacgaggg
     5461 cctcaactcg cccgaccggc tgcagcggcc gctggtgaag cgcgacggca actggcagga
     5521 gctcgactgg gagcaggcgt tcgaggtcct gctgcccaag ctgcagggcg agttcggcgt
     5581 gctcgcttca ccgcacgcca cgctggaaga gctcttcctc gtctccaggc tcggcgcgcc
     5641 cgcggacttc cgcctgcgcc agagcgactt ctccttccgc gccgccccgt ggctcggcat
     5701 gccgatcgcc gagctcgaca agctcgaccg cgtgctcgtg gtcggctcgt tcctgcgcaa
     5761 ggaccacccg ctcatcgcgc atcgcctgcg ccaggcggcg aagcgcggcg cgcagatcca
     5821 catgcttcac tcggtggacg acgactggct catgccgatc gccggcaaga aaatcgtcgc
     5881 gccggcggat ttgccttccg cgctcggcag ctatggcgag ctttttgccg gatccaagaa
     5941 ccccgcggtg ttcctcggca acttcgcgat gcagcacccg cgggcggggg agatcctcgc
     6001 ggcggcgaat gcgctcggcg tgaaggtcgg tgtgttcggc gaggctgcga attcagtcgg
     6061 cggcaccctc gccggtctgc ccagcgcggg caacgtgaac gatgtgctcg gcaagaagag
     6121 catcgttctc ctcaacgtcg agccggagct cgactgcgcc gacccggtcg ccgcgcgcaa
     6181 ggcgctcgac gccgccgagt tcgtcgtgag cctcacgcca tggaagaccg gactcgagta
     6241 cgccgatgtc cttctgccga tcaccccgtt caccgaaagc tcgggcacgt tcgtcaattg
     6301 cgagggccgc gtgcagagct atcacgccac ggtgaatgct ctcggcaatg cgcggccggg
     6361 ctggaaggtg ctgcgcgtgc tggggagcct gcttggccgc gaaggcttcg agctcgacac
     6421 gatcgagcag gtgcgcgcgg cgtgcctgga cggcaaagac attccgaagc tcctttcgaa
     6481 ctcggtgcag gccgccggca gcgcgcgcgc ttcggtgtcg ggcattcagc gcatcgccga
     6541 cgtgccgatc tacttcgccg acccgctcgt gcgccgcgcg ccttcactgc agaaaaccaa
     6601 ggacgcacgc ccgccgcgcg cttggatgaa ttcgaagctg ctcgcgaagc tgggcgccgc
     6661 cgcggggcag ccggtgctcg tgaacggctc ggtcaagctc atggcggcgc tggacgaccg
     6721 gctgcccgac gaatgcgtgc gcatcgccgc cgggcatccg accaccgcgg cggtcgggcc
     6781 gatgttcggc atgctagagc tcgcgcgcct cgcgacggag aaagccgcct aaatggaatg
     6841 gctcgcggag cgctcgacga gctggttcgg cccggtctgg ggtccggtgt tctacgaaac
     6901 gggcaccaac ctcgccttca tcgtggccat cgtggtgccc ctcatgctgg ctgtcgcgta
     6961 cctgacgtta tgggagcgga agctcatcgg ctggatccag atccgcatcg gcccgaaccg
     7021 cgtggggccc ttcggcctgc tgcagccgat cgccgacgcc atcaagctgc tcatgaagga
     7081 gctcatcctc ccgaccaacg cgaacaaggg gctctacctc ctcgcgccgg tgctgatgat
     7141 catgccggca ctcgcggcgt gggccgtgat cccgttctcg cccgagctcg tgctcgcgga
     7201 catcaacgcc gggctcctct acatcatggc gctctcgtcg ctcggcgtgt acggcgtgat
     7261 cgtcgcgggc tgggcgtcga actcgaaata cgcctttctc ggcgcgatgc gctcggcggc
     7321 gcagatggtg tcctacgagc tcgccatggg cttcgcgctc gtcgtggtgc tcatggtgtc
     7381 gggcagcctc aacctctccg acatcgtcaa agggcagggc gagggctact tcgcctcgag
     7441 gggcgtcacc ttcatgtcgt ggaactggct gcccctgctg cccatgctta tcgtttattt
     7501 cgtgagcggc gtggccgaga cgaaccgcgc gccgtttgac gtggtcgagg gcgagtccga
     7561 gatcgtcgcc ggccacatga tcgagtactc cgggatgagc ttcgcgctct tcttcctcgc
     7621 cgagtacatg aacatgctgc tcatcagcgc gctcaccgca gtcatgttct tcggcggctg
     7681 gcaggcgccg atcgacgcgc cggtcttgca cctgattccg cctttcggct ggctgctcgt
     7741 caagatcttc ctcatcgtct ccatgttcct ctggatccgc gccaccttcc cgcgctaccg
     7801 ctacgatcag atcatgcgcc tgggctggaa ggtgttcatt ccgctgacgc tcgtctggat
     7861 cgcggtgatc gcgatctgga tgcagacgcc ctggaacatc tggccatgac gcgcgttctc
     7921 gacttcttca aatcgttcct cttgctcgag ctcctccgcg gcatggcgct caccgggcgc
     7981 cacatgttcg cgcgcaagat cacggtgcag ttcccggagg agaagacgcc gctcagcccg
     8041 cgcttccgcg gactgcacgc gctgcgccgc tatccgaacg gcgaggagcg ctgcatcgcg
     8101 tgcaagctgt gcgaggcggt gtgcccggcg ctcgccatcc acatcgagtc ggagcagcgt
     8161 gccgacggca cgcgccgcac cacgcgctac gacgtcgatc tggtgaagtg catcttctgc
     8221 ggcttctgcg aggaggcctg cccggtggat gcgatcgtcg agacgcgcac gctcgagtac
     8281 catggggaga agcgcggcga cctgtacatg accaagccga tgctgcttgc cgtcggcgac
     8341 cgatacgagg aacaaatcgc ccgcgaccgg gctcaagacg cccgctaccg ctagaccaac
     8401 aaagaataat gttcgaaacc tggcttttct acgccttcgc ggcggtgctg ctcgtgtccg
     8461 ggctgctcgt catcacggcc cgcaaccccg tgcacggcgt gctgtggctc gtgctcgcct
     8521 tcttcaccgc ggcgggcatc tggctcctgc tgcgcgccga gttcctggcg atcacgctgg
     8581 tcgtggtcta cgtcggcgcg gtgatggtgc tctttctctt cgtggtgatg atgctggaca
     8641 tcaacatcga gcgcgtgcgc gaaggcttct ggcgcaacct gccgctcgcg ctcgtcgtcg
     8701 gcggcgtcat ggtgctggaa atggtcgcgg tgctcgccaa ccgctaccac ggcgcggtgc
     8761 cgcgcgacct gccggccggc tacagcaaca ccaaggccct cggccgcgtg ctctatacgc
     8821 agtacgcctt cgcgttcgaa atcgcggcgg tgctgctgct cgtcgccatc atcgccgcga
     8881 tcgcgctcac cctgcgcagg cgcaaggaca gccgctacca ggacccgtcg cggcaggtga
     8941 aagcgcggcg cgaggagcgc gtgcagctcg tctcgatgaa gcccgaggtg gacaagtgag
     9001 cggtccgctc agcctttccc acttcctcgt cctcggcgcg atcctcttcg cgatcagcgt
     9061 ggtcggcatt tttctcaacc gccgcaacct catcgtgctt ctcatggcga tcgagctcat
     9121 gttgctcgcc gtgaacatga acttcatcgc cttctcctac ttcctcggcg acctggcggg
     9181 ccaggttttc gtcttcttca ttctcaccgt ggctgcggca gagtcggcca tcggcctggc
     9241 gatcctcgtc gtcctcttca ggaacctgaa caccatcgat gtcgaagatc tcgatgccct
     9301 caagggctaa gaaataggga cggaccctat tttccgatga aggctctcta tctcatcgtc
     9361 ccgttcgccc cgctcgtcgc ggcgatcgtc gccggcgtgt tcggcaagca ggttggccgc
     9421 gccggggcgc actcggtgac gatcttcgcg gtgctgctct cgttcctttg ctcgtgcgtg
     9481 gtgttctacg acgtgctgca gggcacgcgc ttcaacgggc cggtctacac ctggatgacg
     9541 agcggcgacg ccgccttcca gatcggcttc ctcatcgacc cgctcaccac gctcatgatg
     9601 gtggtggtca cgttcgtgtc gctcatggtg cacatctaca ccatcggcta catgcacgac
     9661 gatccgggct accagcgctt cttctcgtac atttcgctct tcacgtttgc catgctgatg
     9721 ctcgtcatgt cgaacaactt cctgcagctc ttcttcggct gggaggcggt cgggctggtg
     9781 tcgtacctgc tcatcggctt ctggtacacg cgcgagaccg cggtgtacgc gaacctgaag
     9841 gccttcctcg tgaaccgggt cggcgacttc ggcttcctgc tcggcatcgc gctcatcctc
     9901 gcgcagtacg gcacgctcga ttacgccgaa gtgttcgagt acgcgcaggc cgtgccgggc
     9961 gaaatcacgc tcatctgcgt gctgctcttc atcggcgcca tggggaaaag cgcgcagttc
    10021 ccgctgcacg tctggctccc cgattccatg gaaggcccca cgcccatctc cgcgctcatc
    10081 cacgcggcga cgatggtgac cgccggaatc ttcatggtgg cgcgcatgtc gccgctctac
    10141 gagctctccg agaccgcgcg ctcggtggtg ctcgtcatcg gtgcggtcac ggcgttctcg
    10201 atggcgctcg tcgcgctcac gcagtacgac atcaagcgcg tggtcgccta ctcgacgctc
    10261 tcgcaactgg gttacatgac cgccgcgctc ggagcttcgg cctactcggc ggcgatgttc
    10321 cacctgatga cgcacgcctt cttcaaggcg gtgctctttc tcgccgcggg ctcggtgatc
    10381 atcgcgctcc accacgagca ggacatgcgg cgcatgggcg ggctgaagaa gtacatgccc
    10441 tttacctact ggaccatgct gatgggtgcg atctcgtcag cgggcatccc gggcttcgcc
    10501 ggattcttct cgaaggacgc gatcatcgag gcggtgcacc actcggcgac cccgggcgcg
    10561 accttcgctt acgtgctcgt gctggtgacg gtcttcgtga ccgcggcata caccttccgc
    10621 ctcgtcttca tgtccttcca cggtccggag cgtttcgata agcaccatcc gccgcacgaa
    10681 tcaccggcgg tcgtcaccgt gccgctcgtc ctcctcgcga ttccctcggt cgctgccggc
    10741 tgggtcatcg gcgacgtggt tttcggcaat tacttcgccg agagcctgcc cgcgccggac
    10801 ccgaactacc acggccttta ttcgttcatc actcacggcc tcgtctcggt gccgttctgg
    10861 ctggcgcttg ccggcatcgc gacggcgtgg ctcctgtaca tgaagaaacc tcagcttccg
    10921 aaacgcctcg ccatggagct cgggccggcg tactcgatcg tggagcgcaa gtacggctac
    10981 gacgagctct actcctggct cttcgccggc ggggcgaggg cgctcggcaa gggattctgg
    11041 cgcgcgggcg accaggccgt gatcgacggc ctggtcgtga acggctcggc gcgcgtcgtt
    11101 ggctggttct cgggcgtggt tcggcttctc cagtcaggct tcatctaccg ttacgcctcg
    11161 gtcatgcttt tcggcgtggt catcctggcc ttctggttct tgaagaacta agtgcttctt
    11221 agcctcgcga tctggatccc catcctcgcc ggcctggcgg ttttcgcgct gggaggagac
    11281 gagcgcgcgc cggcgcagcg ctggctcgcg ctggcgggcg cagtgctcgg cttcctcgtc
    11341 acgattcccc tctacacggg cttcgatgca tcgaacgccg gattccagtt cgaggagaag
    11401 aagctctgga tcgagacctt caacatccac taccacctgg gcgtggacgg aatctcggtt
    11461 ctcttcgtcc tgttgaacag cttcatcacg atcctcgtcg tgctcgccgg ctggtcggtg
    11521 atcgacaaca aggtcggcca gtactacggc gccttcctca tcatgtcggg gctgctcaac
    11581 ggcgtgttcg ccgcgctcga tgccgtgctc ttctacgtgt tcttcgaggg cacgctgatc
    11641 ccgatgttca tcatcatcgg cgtgtggggc gggccgaacc gggtgtacgc ggcggtcaag
    11701 ttcttcctct acacgctgct cggctcgctg ctcatgctgg tggcgatgct ttacctctac
    11761 aacaaggcgg gcggcagctt ctccatcacc gattggcacc atgtgccgct gccgctcggc
    11821 gtgcagatcg tgcttttctt cggcttcctc ttggccttcg cggtcaaggt gccgatgtgg
    11881 ccggtgcaca cctggctgcc cgacgcgcac accgaggcgc cgaccggcgg ctcggtggtc
    11941 ctggcggcga tcctgctgaa gctcggcgcg tacggcttcg tgcgcttcac cctgccggtg
    12001 ctgcccgacg cctcgcgcga gctcgcctgg ctgatgatcg cgctgtcgct ggtcgccatc
    12061 gcctacatca gcctggtgac cctggtgcag accgacatga agcggctcat cgcctactcg
    12121 tcggtcgcgc acatgggctt ctgcaccctc ggcttcttcc tgttcaacgc ctacggcgtg
    12181 gaaggcgcgc tggtgcagat gatctctcac gggttcgtct cggcggcgct gttcctctgc
    12241 atcggcgtga tgtacgaccg catgcactcg cggaacatcg ccgactacgg cggggtggtg
    12301 aacaccatgc cgaagttcgc cgcgctgatg atgctgttcg ccatggccaa ctgcggcctg
    12361 ccggcgacca gcggcttcgt c
//
LOCUS       DASTPD010000297         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1385231, whole genome shotgun sequence.
ACCESSION   DASTPD010000297 DASTPD010000000
VERSION     DASTPD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385231"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFR57_09895"
     CDS             complement(<1..903)
                     /locus_tag="VFR57_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEU0259714.1"
                     /translation="MSNDRKIIAVIGATGAQGGGLVRAILKDPAGGFAVRAVTRDVNS
                     PKAKALARLGAEVVAADIDDATSVERAFQGVYGAFCVTFFWDHFSPEKELDQARILAR
                     AAKRAGVKHAIWSTLEDTRKWVPLSDARMPTLNGKYKVPHFDGKGEADEEFRSLGVPT
                     TFLLTSFYWDNLIHFGMGPTRGADGRLAFTLPMGERALPGIAAEDIGRCAYGIFKRGA
                     ELIGQTVGVAGEHLTGAQITAALRGALGEEVVHNAISPEAYRKLGFPGAEDLGNMFQF
                     NRDFNEDFRRARDPSVARALNPALQ"
     gene            1006..1983
                     /locus_tag="VFR57_09900"
     CDS             1006..1983
                     /locus_tag="VFR57_09900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0259715.1"
                     /translation="MAMDQFFCMRVFARVVELGSFARASEALDIGRASATTAVARLEK
                     RLGVRLLHRTTRRLSLTDDGRLYYQACVTMLADLAEAEDVLSNARRSPRGRLRVSTPH
                     SFIHLVFLSALPKFLARYPDLEVEVVMTDRAVNLVEEGIDCAIRAVDIPADSPLVARR
                     ISSLRWLTCASPSYLKARGTPASVADLERHECIRSISPSTGRTANWRFEKNGERQDFT
                     PRGRVGVNSLEAAGAAAAYGMGIAQVTEVLVAPRLRSGELRPLLTDWAAAAPPLMVVY
                     PSNRYLSAKVRAFGDFVAEVFPREGLWPERSLASAARARPKKASSANRR"
     gene            complement(2005..2580)
                     /locus_tag="VFR57_09905"
     CDS             complement(2005..2580)
                     /locus_tag="VFR57_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0259716.1"
                     /translation="MPVVYLTGAAATGKSTLARNLRALCPELVVFSYSERLRDLIASR
                     QGGLHEDEARRKSAGIVTPADIETLDNSLLNLVTYERASRSILIDSHAITKEDFGFRV
                     TGVSTERIKALNPDAIVCLYAHGQAVIDRMRTQATGPLMSLFEADMQTYMQAQLAIQC
                     GVIVGRPVYLFDAGMPADNLAKVVAEKTKLI"
BASE COUNT          459 a          883 c          812 g          502 t
ORIGIN      
        1 ctgcaacgct gggttgagcg cgcgcgcgac cgacgggtcg cgcgcgcggc ggaagtcctc
       61 attaaagtcg cggttgaact ggaacatgtt gccgagatcc tcggcgccgg ggaagcccaa
      121 cttgcggtaa gcctccgggg agatcgcgtt atgcacgacc tcctcgccga gcgcgccgcg
      181 gagcgctgcg gttatctgcg caccggtaag gtgctcgcct gcgacgccga ccgtctggcc
      241 aatgagctcg gcgccgcgct tgaagattcc gtaagcgcag cgaccgatgt cctcagcggc
      301 gatgccggga agcgcccgct cgcccatagg cagcgtgaag gcgagcctgc cgtccgcgcc
      361 gcgcgtcggc cccatgccga aatggatcag gttgtcccag tagaacgacg tgagaaggaa
      421 cgtcgtcggc acgccgaggc tgcggaactc ctcgtccgcc tcgcctttgc cgtcgaaatg
      481 cggcactttg tacttcccgt tcagagtcgg catacgcgcg tccgacagcg gcacccattt
      541 gcgcgtgtcc tcgagagtcg accagatcgc gtgcttcacc ccggcgcgtt ttgccgcgcg
      601 ggcgagaatg cgcgcctggt cgagctcttt ctctggggag aagtggtccc agaagaacgt
      661 tacgcagaac gctccgtaca cgccctggaa tgcgcgttcc acgctcgtgg cgtcgtcgat
      721 gtccgcggcg acgacctcgg cgccgagccg ggcaagcgcc ttcgctttgg gtgagttgac
      781 gtctcgggtg actgcgcgca ccgcgaagcc gcccgccggg tccttgagaa tggcgcgcac
      841 caggccgccg ccctgcgcgc cggtcgcgcc gattaccgca ataatcttcc tgtcgttgct
      901 catttccgcc tccatgtagg ctgaagttgg atggcgcact gtattagccc cggcttgcca
      961 aataaaccgc cgcgcggcaa attgattgtt cttcaagcgc aaacaatggc gatggaccaa
     1021 ttcttctgca tgcgggtatt cgcgcgcgtc gtcgagcttg gctccttcgc gcgcgcctcc
     1081 gaggcgctcg acatcgggcg ggcgagcgcg accactgccg ttgcacgcct tgagaagcgc
     1141 ctcggcgtgc ggctgctcca ccgcacgacc cggcgcctca gccttaccga cgatggtcgg
     1201 ttgtactacc aggcctgcgt caccatgctt gcggatctgg cggaagcgga agacgtgcta
     1261 tccaacgcgc gcagatcccc gcgcgggcgc ctgcgcgtca gtacgccgca ttcgttcatt
     1321 cacttggtgt tcctctccgc gctaccgaag ttcttggcgc gctacccgga cctcgaagtc
     1381 gaggtggtga tgacggaccg agccgtcaac cttgtcgaag aaggaatcga ctgcgcaata
     1441 cgcgcggtcg acatcccggc cgattcgccg ctggtagcgc ggcgaatctc cagcctccgt
     1501 tggctcacct gcgcttcgcc ctcctacctc aaggcgcgcg gcacgccggc gagcgtggcg
     1561 gatctcgagc gccacgaatg catacgttct atctcgcctt ccaccggccg aaccgccaat
     1621 tggcgtttcg aaaagaacgg cgagcgccag gactttacgc cgcgcggacg cgtcggcgtg
     1681 aactcgctgg aagccgccgg cgccgcggcc gcgtacggca tgggcatcgc ccaagtgacc
     1741 gaagtgctgg tcgcgccgcg cctgcgctcc ggcgaattgc ggccgctgct taccgactgg
     1801 gcggcggccg ccccgcccct catggtggtc tacccgagca accgttattt gagcgccaag
     1861 gtgcgcgcgt tcggcgattt cgtcgccgag gtctttccgc gcgagggcct atggcccgaa
     1921 cgcagtctcg ccagcgcagc acgcgcccgg ccgaagaaag cctcgagcgc taaccgccgt
     1981 tgatctcttg cagggcagcc tgatctagat cagctttgtc ttctcagcaa ctaccttcgc
     2041 caggttgtcc gctggcattc cggcgtcaaa gagataaaca ggcctgccca cgatcacacc
     2101 acactggata gcaagctgag cctgcatgta cgtttgcatg tcagcctcga aaaggctcat
     2161 cagcggtccc gttgcctggg tcctcatgcg gtcgataact gcctgcccat gagcgtacag
     2221 acaaacgatt gcgtccgggt tgagcgcctt gattcgctct gtagaaacgc ccgtcacgcg
     2281 aaagccaaag tcttctttcg ttatcgcatg cgaatcgatg agaatcgacc tgctggcacg
     2341 ttcgtacgtc accaggttca agagcgagtt atccaaggtt tcgatgtctg ccggtgtaac
     2401 gatccccgca gattttcgcc ttgcttcatc ctcgtgcaag ccgccttggc ggctggcgat
     2461 cagatcacgt agccgttcac tgtacgagaa gaccacgagc tcgggacaca gtgcgcgcag
     2521 gttgcgcgcg agcgttgact taccagtagc cgcagcgcca gtgagataaa caactggcac
     2581 aagagctaac gcaaccctgc gttgatctgc tccagcatcc cccctcccgg gcccaggtcc
     2641 ttctcggcga ggttat
//
LOCUS       DASTPD010000298         9264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_157890, whole genome shotgun sequence.
ACCESSION   DASTPD010000298 DASTPD010000000
VERSION     DASTPD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157890"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFR57_09910"
     CDS             complement(<1..379)
                     /locus_tag="VFR57_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259717.1"
                     /translation="MTAGVLWLLLALFVLRVLGQALVAFLDVGFLPPMAAWYSGLMPY
                     QYLLPSQIAIVALMAKISVDFTRHSGFFYEAKKLFATAWLWFGYLYLAAMVARAVLFW
                     DRPIPIVFHWVLAAFVIAVGRSHR"
     gene            418..903
                     /locus_tag="VFR57_09915"
     CDS             418..903
                     /locus_tag="VFR57_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259718.1"
                     /translation="MGLAVAASAAAAADVGAAGAFSRGRMHFVVTAGSGSAFDESYLV
                     LGIGASYYLVDGLSVGAHYESWSGADPDLSKFTGSLQYVFHQMQAVKPYLGGFYRRTN
                     VGGLPDLDSVGGRAGVYIGAGRNAYLGLGVVYESYLDCNETVYRSCDSTYGEVTFTIA
                     F"
     gene            complement(900..2333)
                     /locus_tag="VFR57_09920"
     CDS             complement(900..2333)
                     /locus_tag="VFR57_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0259719.1"
                     /translation="MLPQAAKKDKPPPGILVNDIHSQLNSSRVWRIVQPDTLDGIRAA
                     LRAAQKEEKAVCISGARHAMGGQQFLADGLMIDTRRMNRLLNFDAEKGHVEFEAGIQW
                     PQILTHLTSLQKERERQWTFAQKQTGADKLTLGGCLSANVHGRGLKMPPFIGDVESFR
                     LLTARGDVVNCSRTENPDLFRLAIGGYGLFGLITSVTLRLVERRKIERVVEVRSIDGL
                     PKAFVERIGEGFIYGDFQYAIDETSDDFLRRGVFTCYRAVDPATPIAPGQKELKERDW
                     VELLTLAHHAKREAFRRYAAHYVATNGQVYWSDEAQMSIYPENYHRALDQRVGAAVKA
                     TEAITEIYCERDALERFMTDMRDYARRDKVDIIYGTVRLIEQDRESFLAWARKPYACV
                     IFNVHVEHTTSGLIKAGDVFRRLIDIGLKHGGSYFPTYNRYALRRQVEACYPQLQEFL
                     KLKLKYDPKELFQSEWYRHYKRMFEKS"
     gene            complement(2452..2772)
                     /locus_tag="VFR57_09925"
     CDS             complement(2452..2772)
                     /locus_tag="VFR57_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259720.1"
                     /translation="MSAPGPSKDAQASSAKDMSVKDPYVDLAERIFVQLSSRVYGTLA
                     GSETKKPDPKALAAFSFRLADAFEQAARETDRMKAKIEAASKAAVKLDEVDLSHVFQS
                     SAEK"
     gene            complement(2769..4568)
                     /locus_tag="VFR57_09930"
     CDS             complement(2769..4568)
                     /locus_tag="VFR57_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEU0259721.1"
                     /translation="MEHGNDFLLQASLYFAAAVAAVMISHKFGLGSVAGYLLAGIAIG
                     PWGFQLVEADAHIKAFAELGVVFLLFVIGLELEPKRLWSMRAKLLGLGLSQVLGSMAA
                     ITAAGWALGLDLRVALIAAMALSLSSTALALAPLKERGAFGTQAGQGAFAVLLFQDIA
                     VIPMLALVPLLGTGAGGSTFSWQKLSLAAAVIVLTLGGGRLVARPVFRHIARTRLREI
                     FTAFALLLVLVIALAFEHVGLSMALGAFLGGVLLAESEYRHEIEAAVEPFKGLLLGLF
                     FIALGLSVDFSVLIAKPILVFALIAGVFAVKGAVLWLIARAARFPRAERPLFILFLAQ
                     GGEFAFVILGVAAMHGGMAADTAQALILAVAISMLLTPFILALHDRLAPWFAAPVEER
                     APDVPQASKVLVAGLGRIGQVVARMLHAAGHEPTMLDDNLDHVEQSRKFGFRVFYGDA
                     TRLDLLHAAGAGEADLLVIALEDADATSRLARIVKTHFPKLRIIARARDMRHMFELRD
                     AGVQTIERETWLAALKLGELALAQASGDPERARRTAEIFARHDVKVVAKLYEVHRTQP
                     DAHISVSHELREQLARTLSEDERRIAVIRAERP"
     gene            4605..4898
                     /locus_tag="VFR57_09935"
     CDS             4605..4898
                     /locus_tag="VFR57_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259722.1"
                     /translation="MDSAEAQLEGWVARLFARRPELWRFTVRDRSGLPDHIDPTTLTG
                     EFFIFEIGISPRRDKAHYDEVYDDIARAIAAAVAAQPAARTLLPGRTFVRALH"
     gene            4908..5696
                     /locus_tag="VFR57_09940"
     CDS             4908..5696
                     /locus_tag="VFR57_09940"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEU0259723.1"
                     /translation="MQAGIRYTLPTGEKLVNETFGPNNIRRRTSGTEQVFPVEIEDGR
                     NKAFSLEENGFVFVRHETAVRDFFDADELEKVYYPEVAALITKLTGAKRVQVFDHTLR
                     SGDEAEREARLLREPVLSAHNDYTEWSGPNRVREVMGAEADSLLQRRFSIIQLWRAIN
                     RPIESNPLAIADARSIPCEDLLIAERRYPHRVGQTYRLQYSPGHRWFYFPRMRRDEAL
                     VFKVYDSAQDGRARFTPHTSFDDPSTPPGAPPRQSIEARALVFF"
     gene            complement(5693..6946)
                     /locus_tag="VFR57_09945"
     CDS             complement(5693..6946)
                     /locus_tag="VFR57_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259724.1"
                     /translation="MLSGLADKKIFYGWWMVGAATGLQFLQAGLVTQAFGAYLAVLAD
                     EQGWSKTALSGAAALQQMEVALLGPVLGWLIDRFGPKVFVRAGVVVFGTGLCLLSQVQ
                     SLPEFYGAFIVIALGSSLCGFFPLNVALIHWFERKRARALSAMSIGLAFGGIMVPLVA
                     QSLTAFGWRATALVSGLAAIAVGLPLALVIRNSPREHGEVVDGMPTEISPTNTSPEKN
                     DSTRDFTAREALRTQAFWLISLGHGFALFVVHAVSVHAITHLKEGLGYSVAAASLVIS
                     LVTTCQIGGVLLGWAIGDRYKKRLIAAACMLAHATALLLLTYAVSLPMVIAFAILHGT
                     AWGLRGPMMQAMRADYFGRSSIGMILGLSFMIIVVGQVGGPIIPGVFADITGDYRVGF
                     TILALLAGLGSGFFLLARKPKRPKL"
     gene            6980..7309
                     /locus_tag="VFR57_09950"
     CDS             6980..7309
                     /locus_tag="VFR57_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259725.1"
                     /translation="MKPAPLGALNADLAPIEEDLAARIDSIFERCPQLFGFTVQDSTA
                     LPEELRSFALERELVVSDIGVYPYLNADQCEQIYNEIAVALLDFLLERPGGKDALRGR
                     TFARSLH"
     gene            complement(7323..8183)
                     /locus_tag="VFR57_09955"
     CDS             complement(7323..8183)
                     /locus_tag="VFR57_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259726.1"
                     /translation="MARFLITPHFRLHEWVAEEKGYFRAEGLDYEFREVFKTHATPDK
                     VGAMTSFEEGRQTNVSCACHWTVNVAASQGRGRMYADAYSVSPSGVFVPPESPIREPK
                     DLKGVPISVGHQSGSHYSTLQALEQYLPREDIKLSFADGILFGRMEKLIDRQVPAAAL
                     FSGPYYFAEQLGFRKIIDTTFMIASMITGDPDPEDVRKYFRALRRAQRDIDLRPELYT
                     HHYAKEFPRRFGGQIDTRRWGPGERIVFEPYTREVFEQSFKWIAEHEIFKPAEMGSGR
                     YEDACVSLQP"
     gene            8362..8616
                     /locus_tag="VFR57_09960"
     CDS             8362..8616
                     /locus_tag="VFR57_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259727.1"
                     /translation="MKTSRHLREQLESLFARCPDLCGFAVRQEAQELYVSDIGISPRL
                     SAEQYGEIYQDIAQTLGELLEEEPRAGEWLRGRTFARTIH"
     gene            8723..9046
                     /locus_tag="VFR57_09965"
     CDS             8723..9046
                     /locus_tag="VFR57_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259728.1"
                     /translation="MKTPQQYDSLFGRLEADVHARIRSLFARCPTLHGFAVQDRAMLP
                     KDIDRDRIPDADLFVTDIGLYPKIESQCDEIHDEITLAITDLVHDQPRAYDYLRGKTF
                     ARVLQ"
     gene            complement(9051..>9264)
                     /locus_tag="VFR57_09970"
     CDS             complement(9051..>9264)
                     /locus_tag="VFR57_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEU0259729.1"
                     /translation="VYAVYVDETDKVWAAEWSAQVMLRFDPTTEKFESFKSSSSRPNV
                     RQIHGRKNEVWTPESGADKIVVYRYK"
BASE COUNT         1657 a         2991 c         3071 g         1545 t
ORIGIN      
        1 gacggtgcga gcggcccacc gcgatgacga aggcggcgag cacccagtgg aaaacgatcg
       61 ggatcggccg gtcccaaaaa aggacggcgc gcgccaccat cgcggcgagg taaaggtagc
      121 cgaaccagag ccaggcggtg gcaaatagct ttttcgcttc gtagaaaaag ccgctgtggc
      181 gcgtgaagtc gaccgagatc tttgccatca gagcgacgat cgcgatctgc gacggcagca
      241 ggtattggta gggcatgagg cccgaatacc acgccgccat tgggggaaga aaaccgacgt
      301 caaggaaggc gacgagtgcc tgcccgagca cccgcagcac gaagagtgcg agcagaagcc
      361 acaacacccc ggccgtcatg cgaaaattag atcatgtatg cgagagcact ggcggccgtg
      421 ggcttggccg tggccgcgtc ggctgccgct gcggccgatg taggcgcggc gggagcgttc
      481 agtcggggcc gcatgcactt tgtggtgact gccggcagcg gctcggcctt cgacgagagc
      541 tacctggtcc tggggatcgg cgcgagctac tacctggtgg acggattgag cgtcggggcg
      601 cactacgagt cctggtccgg cgcggacccc gatctcagca agttcaccgg ctcgctgcag
      661 tacgtctttc accagatgca ggcggtaaag ccttatctcg gcggtttcta tcggcgcacg
      721 aatgtgggcg gcttgccgga cctcgattcg gtcggcgggc gcgcaggtgt ctatatcggc
      781 gcgggacgca acgcctacct cggtctgggg gtggtctacg agtcctacct cgactgcaac
      841 gagaccgttt accgctcctg cgacagcacc tacggcgagg tgactttcac tattgcgttc
      901 tagctcttct cgaacatcct cttgtaatga cggtaccact cgctctggaa gagctccttg
      961 gggtcgtatt tgagcttgag cttgaggaat tcctgaagct gcgggtagca cgcttcgacc
     1021 tggcggcgta gtgcgtagcg gttgtaagtg gggaagtagc tgcccccgtg cttgaggccg
     1081 atgtcgatca ggcggcgaaa cacgtcgccc gccttgatca ggccgctcgt ggtgtgctcg
     1141 acgtgcacgt tgaagatgac gcatgcgtag ggcttgcgcg cccacgcgag gaagctctcg
     1201 cgatcctgct cgatcagccg caccgtgcca tagatgatgt cgaccttgtc gcgccgcgca
     1261 tagtcgcgca tgtccgtcat gaagcgctct agcgcatcgc gctcgcagta gatttcggta
     1321 atcgcttcgg tcgcctttac cgccgcgccg acacgctggt cgagcgcgcg gtggtagttc
     1381 tccgggtaga tgctcatctg ggcctcatcg gaccagtaca cctgaccgtt ggtcgccacg
     1441 tagtgcgcgg cgtagcggcg gaatgcctcg cgcttggcgt ggtgcgcgag cgtgagcagc
     1501 tcgacccagt cgcgctcttt cagctccttc tgcccgggcg cgatcggcgt cgccggatct
     1561 accgcgcggt agcacgtgaa tacgccgcgc cgcagaaagt cgtccgaggt ttcgtcaatc
     1621 gcgtattgga agtcgccgta gatgaagccc tcgccgatgc gctcgacgaa cgccttgggc
     1681 aggccgtcga tggagcggac ctcgaccacg cgctcgatct tgcggcgctc gaccaggcgc
     1741 agcgtcaccg acgtgatcag gccgaacagg ccatagccgc cgatcgcgag gcggaacagg
     1801 tctgggttct ccgtgcggct gcagttcacc acgtcgcctc tcgccgtgag caggcggaag
     1861 gactcgacgt cgccgatgaa gggcggcatc ttgagcccgc ggccatgcac gttcgccgac
     1921 aggcagccgc caagggtgag cttgtcggcg ccggtctgct tctgtgcgaa ggtccactgg
     1981 cgctcgcgct ccttctgcag gctggtgagg tgggtaagga tctgaggcca ctggatgccg
     2041 gcctcgaact cgacgtgccc cttctcggcg tcgaagttga gcaggcggtt catgcgccgc
     2101 gtgtcgatca tcagcccgtc ggcgaggaat tgctggccgc ccatcgcgtg gcgcgcgccg
     2161 gagatgcaga ccgctttctc ctctttctgg gccgcgcgca gcgccgcgcg gatgccgtcc
     2221 agcgtgtcgg gctgcacgat gcgccatacg cgcgacgagt tgagctgcga gtggatatcg
     2281 ttgaccagga tgcccggcgg cggcttgtcc ttcttggccg cctgcggaag cacactggag
     2341 ggccacgcgg cgccggcgag cgtgattccc aggaaatcgc ggcgccgcag caggcctaga
     2401 gctccccgcg gccgattgtt tcgagcgcag acaacgccat gccggttccc gttatttctc
     2461 ggcgctgctc tggaagacgt gcgacaagtc gacttcgtcg agcttcaccg cggccttgct
     2521 tgccgcctcg atcttggcct tcatgcggtc ggtttcccgc gccgcctgct cgaaggcgtc
     2581 ggcgagcctg aagctgaacg cggcgagcgc cttggggtcg ggttttttcg tctcggagcc
     2641 ggcgagcgtg ccgtaaacgc ggcttgagag ctgcacgaag atgcgctcgg cgagatcgac
     2701 gtaggggtcc ttgacgctca tgtccttcgc gctcgacgcc tgcgcgtcct tgctgggtcc
     2761 cggtgcgctc atggtctttc cgctcttatg accgcaatcc tacgctcatc ttcgctcagc
     2821 gtgcgagcaa gctgctcgcg cagctcgtgg gaaacgctga tgtgggcgtc gggctgggtg
     2881 cggtgcacct cgtagagctt cgccaccacc ttgacgtcgt gccgggcgaa tatctccgcg
     2941 gtgcggcgcg cgcgctccgg atcgccgctc gcctgagcga gcgcgagctc gccgagcttg
     3001 agtgccgcaa gccaggtctc gcgctcgatc gtctgcacgc ccgcgtcgcg cagctcgaac
     3061 atgtgacgca tgtcgcgggc gcgcgcgatg atccgcaact tggggaaatg cgtctttacg
     3121 atccgcgcga gacgtgaggt ggcgtcggcg tcttcgagcg cgatcacgag caggtcggct
     3181 tcgccggccc ccgcggcgtg cagcaggtcc aggcgtgtcg cgtcgccgta aaacacgcgg
     3241 aagccgaact tgcgcgactg ctcgacgtgg tcgagattgt cgtcgagcat ggtcggctcg
     3301 tggcccgcgg cgtgcagcat gcgcgccacc acctggccga tgcgccccag gccggcgacg
     3361 agcaccttgc ttgcctgggg cacgtcgggc gcgcgctctt cgaccggcgc ggcgaaccac
     3421 ggggcgaggc ggtcatgcag cgcgaggatg aacggggtga gcagcatcga gatcgcgacc
     3481 gcgaggatga gcgcctgagc cgtgtctgca gccatgccgc cgtgcatggc tgcgacgccg
     3541 aggatgacga aggcaaactc gccgccctgc gccagaaaca ggatgaagag cgggcgctcg
     3601 gcgcgcggaa agcgcgccgc acgcgcaatg agccacagca ccgctccctt gacagcgaac
     3661 acgcccgcga tcagggcgaa gacgaggatg ggctttgcga tgagcaccga gaaatcgacg
     3721 ctcaagccga gcgcgatgaa gaacaggccg agcaggaggc ctttgaatgg ctcgaccgcc
     3781 gcctcgatct cgtggcggta ttccgactcg gcgagcagca cgccgccgag gaacgcgccg
     3841 agcgccatgg agagcccgac gtgctcgaag gcgagggcga tcacgagcac gagaagcagc
     3901 gcgaacgcgg taaagatctc gcgcaggcgc gtgcgcgcga tgtggcggaa cacgggccgc
     3961 gccaccaggc ggccgccacc gagcgtgagc acgatcacgg ccgccgccag gctgagcttc
     4021 tgccaggaga aggtcgagcc cccggctccg gtgccgagga gcggcaccag cgcgagcatt
     4081 gggatgacgg cgatgtcctg gaacagcagc actgcgaacg cgccctggcc ggcctgcgtg
     4141 ccgaacgcac cgcgctcctt cagcggggcg agcgcaagcg cggtggagga gagcgagagc
     4201 gccatcgccg cgatcagcgc gacgcgcagg tccaagccga gcgcccagcc cgccgccgtg
     4261 atcgccgcca tcgagccgag aacctgggaa aggccgagac cgagcagctt ggcacgcatg
     4321 ctccagagcc gcttcggctc gagctccagg ccgatcacga agagcaggaa aaccacgccg
     4381 agctcggcga aggccttgat gtgggcgtcg gcctcgacca gctggaagcc ccagggcccg
     4441 atcgcgattc cggcgaggag atagcctgcc accgacccca ggccgaactt gtgagaaatc
     4501 atcacggcgg ccaccgcggc cgcgaaatag aggctcgcct ggaggagaaa gtcgttgccg
     4561 tgctccacgg ccgaagtata ttggcggcgg gagggttgat agaaatggac tccgccgaag
     4621 cgcagctcga ggggtgggtc gcgcgcctct tcgcgcgccg ccccgaattg tggcgcttca
     4681 ccgtacgcga ccgcagcggg ctgcccgacc acatcgatcc gaccacgctc accggcgaat
     4741 tcttcatctt cgagatcgga atctccccgc ggcgcgacaa ggcgcactac gacgaggtgt
     4801 acgacgacat cgcgcgcgcc atcgccgcgg cggtggcggc gcagccggcc gcgcgcacgc
     4861 tgctcccggg acgcacgttc gtaagagccc tgcattagac tagccgggtg caggccggca
     4921 tccgctacac ccttcccacg ggcgagaagc tcgtcaacga gaccttcggg ccgaacaata
     4981 tccgtcgccg gacctccggt acggagcagg ttttcccggt ggaaatcgag gacgggcgca
     5041 acaaggcctt ttccctggaa gagaacggct tcgtcttcgt gcggcatgag accgcggtga
     5101 gggacttctt cgatgcggac gagctcgaaa aggtctatta cccggaagtc gccgcgctca
     5161 tcacgaagct gacgggcgcc aagcgcgtgc aggtcttcga tcacacgctg cgctcgggcg
     5221 acgaggccga gcgcgaggcg cggcttctgc gcgagccggt actctcggcg cacaacgact
     5281 ataccgagtg gtccgggccg aacagggtca gggaagtcat gggcgccgag gccgattcct
     5341 tgctgcaacg ccgtttctcg ataattcagc tgtggcgggc gatcaaccgg ccgatcgagt
     5401 cgaacccgct ggcgatcgcc gacgcgcgca gcatcccgtg cgaggacttg ctcatcgcgg
     5461 agcgccgcta tccgcaccgc gtcggccaga cctatcgcct ccagtacagc ccgggtcacc
     5521 gctggttcta cttcccgcgg atgcggcgcg acgaggcgct tgtcttcaag gtctacgact
     5581 cggcgcagga cggccgcgcg cggtttacgc cgcacacctc gttcgacgat cccagcacgc
     5641 cgccgggcgc gccgccgcgg caaagcatcg aggcgagggc actggtcttt ttttaaagct
     5701 tgggtctttt cggctttcta gcgagcagga agaagccgga gcccaggccc gcgagaagcg
     5761 ccaggatggt gaagccgacc cggtagtcgc ccgtgatgtc ggcgaagacc cccgggatga
     5821 tcgggccgcc gacctggccc actacgatga tcatgaacga gagcccgagg atcatgccga
     5881 tcgagctgcg gccgaagtaa tcggcgcgca tcgcctgcat catcggcccg cgcaagcccc
     5941 aagcggtgcc gtgcagtatc gcgaaggcga tcaccatcgg aagcgagacc gcgtaagtca
     6001 gcagcaacag cgcagtcgca tgcgcgagca tgcacgccgc agcgatcagg cgcttcttgt
     6061 agcggtcgcc gattgcccag ccgagcagca cgccgccgat ctggcacgtc gtcaccaggc
     6121 tgatgacgag cgaggcggcc gccaccgagt agccgagccc ttccttcagg tgcgtgatcg
     6181 cgtgcaccga gactgcgtgc acgacaaaga gcgcgaagcc gtgcccgagc gagatcagcc
     6241 agaacgcctg cgtgcgcagc gcctcgcgcg cggtgaagtc gcgcgtcgaa tcgtttttct
     6301 caggagaagt attcgtcggc gagatctcgg ttggcatgcc atccaccact tctccgtgct
     6361 cgcgggggct gttgcgaatc accagggcaa ggggcaggcc gaccgcgatc gcggccaggc
     6421 ccgaaacgag ggcggtggcg cgccagccaa acgcggtcag cgactgcgcg acgagcggca
     6481 ccatgatgcc gccgaaggcg agcccgatcg acatcgccga gagcgcgcgg gcgcgcttgc
     6541 gctcgaacca gtgaatgagc gcgacgttga gcgggaaaaa tccgcacagg ctcgagccga
     6601 gcgcgatgac aatgaaagcg ccgtagaact cgggtagcga ttgcacctgc gagagcaggc
     6661 aaagaccggt gccgaacacc accacgccgg cacgcacgaa gaccttcggg ccgaagcggt
     6721 cgatcagcca gccgagcacc gggccgagca gcgcgacttc catctgctgc agcgccgccg
     6781 cgcccgagag cgcggtcttg ctccagccct gctcgtcggc gaggactgcc aggtaggcgc
     6841 cgaacgcctg ggtgacgagc cccgcctgca ggaactgcag gccggtcgcg gcaccgacca
     6901 tccaccagcc gtagaagatt ttcttgtcgg ccaaccccga cagcataact tcgggtccgt
     6961 tatttgcggc aaccgcggca tgaaacccgc tccgctaggc gccctcaacg ccgacctggc
     7021 gcccatcgag gaggatctgg ccgcgcgcat cgattcgatc ttcgagcgct gccctcagct
     7081 cttcggcttc accgtacagg actccacggc actgcccgag gagcttcgct ccttcgcgct
     7141 cgagcgcgag ctggtggtga gcgatatcgg cgtctatccc tatctgaacg ccgaccagtg
     7201 cgagcagatc tacaacgaga tcgcggtcgc gctgctcgac ttcctattgg agcgcccggg
     7261 aggcaaggac gcgctgcggg gccggacctt cgcccgcagc ctgcactgaa agaaacaggc
     7321 cgttagggct gtagcgaaac gcaggcgtcc tcgtaccgcc cagagcccat ttcggccggc
     7381 ttgaaaatct cgtgctcggc gatccatttg aaggactgtt cgaacacttc acgcgtataa
     7441 ggctcgaaca cgatgcgctc gcccggtccc cagcgccgcg tgtcgatttg tccgccgaag
     7501 cggcgcggga attccttggc gtagtggtgg gtgtagagct cgggacgcag gtcaatgtcg
     7561 cgctgcgcac ggcgcagcgc gcggaaatac ttgcgcacgt cctcggggtc ggggtcgccg
     7621 gtgatcatcg aggcgatcat gaacgtggtg tcgatgatct tgcggaagcc gagctgctcg
     7681 gcgaagtagt aaggaccgct gaagagcgcg gccgccggca cctggcggtc gatcagcttc
     7741 tccatgcggc cgaagaggat gccgtcggcg aacgagagct tgatgtcctc gcgcggcagg
     7801 tactgctcga gcgcctggag cgtcgagtag tggctgcccg actgatggcc caccgagatc
     7861 ggcacgccct tcaggtcctt cggctcgcgg atcggtgact ccggcgggac gaataccccg
     7921 ctcggggaga ccgagtaggc atcggcgtac atcctgccgc ggccctgcga ggcggcgacg
     7981 ttcaccgtcc aatggcacgc gcagctcacg ttggtctggc gcccctcctc gaagctcgtc
     8041 atcgcgccga ccttgtcggg cgtggcgtgg gtcttgaaga cctcgcggaa ctcgtagtcg
     8101 agtccctcgg cacggaaata tcccttctct tcggctaccc attcgtgaag ccggaagtgc
     8161 ggggttatga gaaacctggc catgcagagc ctccggggcc gtgaataacg cccacgaata
     8221 ctaatgcgct gggggcgcac tgggtcatgt tgctgtagtc ggtttcgcga tacggcattc
     8281 aacgacaggg aaaacaccta aaccaggcgg accccccaaa ggacaggggc ggtgcaacgc
     8341 accaagacta ccgtagacgc catgaaaacc tcgcgtcact tacgcgagca gctggagtcg
     8401 ctctttgcgc gctgccccga tctgtgcggg ttcgcggtcc gccaggaggc ccaggaactc
     8461 tatgtgagcg acatcgggat ttcgccgcgc ctttcggcgg agcagtacgg cgagatctac
     8521 caggacatcg cccagaccct tggtgagctg ctcgaagagg aaccccgagc cggcgagtgg
     8581 ctccgcggcc gtaccttcgc ccggaccatc cactagaaaa atcgggctct gaccccgatt
     8641 ttcggggtct cggggttcgg atgtagttct ttcagggatt cccctatagg tctttttgct
     8701 acaagacctt aggctggagc gcatgaaaac tccgcaacag tacgacagcc tctttggccg
     8761 gctggaggcc gacgtccacg cccgcatccg ctcgcttttc gcccgttgtc cgacgcttca
     8821 cggcttcgct gtccaggatc gcgccatgct ccccaaggac atcgaccgcg accgcattcc
     8881 cgatgccgat ctcttcgtga ccgacatcgg cctgtacccg aagatcgaat cgcagtgcga
     8941 cgagattcac gacgagatca ccctcgccat cacggacctc gtgcacgacc agccgcgtgc
     9001 ctacgattac ctgcggggca agactttcgc tcgggtactc cagtagcaat ttatttgtag
     9061 cgatacacca cgatcttgtc ggcacccgac tcgggggtcc acacctcgtt cttgcggccg
     9121 tggatctggc gaacgttcgg acgggagctc gaggacttga agctttcgaa tttctccgtc
     9181 gttgggtcga agcgcagcat cacctgcgcg ctccactcgg cggcccacac cttgtcggtt
     9241 tcatcgacat agaccgcgta gacg
//
LOCUS       DASTPD010000299        10692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_976653, whole genome shotgun sequence.
ACCESSION   DASTPD010000299 DASTPD010000000
VERSION     DASTPD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976653"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFR57_09975"
     CDS             complement(<1..313)
                     /locus_tag="VFR57_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259730.1"
                     /translation="MSAYNKVRSAATGIALAMLVSCGGGGGGGGGGGGGTPPLQYSGN
                     VSAAVITATNAGTLTANVAGGGSGLDSATSGVGGSQVQAGQIDIGRGLARAVRSTAKR
                     PD"
     gene            complement(375..848)
                     /locus_tag="VFR57_09980"
     CDS             complement(375..848)
                     /locus_tag="VFR57_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259731.1"
                     /translation="MKKVKPVPDGYHTVTPYLACADAAQAIAFYKKAFGAKELLRVPG
                     PGGKIGHAELRIGDSRVMLADEYREMDFLGPLSRGGSAVHLHIYMPNADKVVERAVEA
                     GAKVVQPLEDKFYGDRTGSVQDPFGHVWHIATHVEDVPPRELEKRAEKMMKDRGA"
     gene            954..1304
                     /locus_tag="VFR57_09985"
     CDS             954..1304
                     /locus_tag="VFR57_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259732.1"
                     /translation="MRLRAPLLLCLLLAACTVAWDERHLEATIVRPSNFKAGSGVIQS
                     VGVLRGQREAGTGVDADGKHPDRNLYRLLIHMDAGGVQTVDIDSSRFLAGEAVELTND
                     GRVVRVSGTTLIQK"
     gene            complement(1308..2021)
                     /locus_tag="VFR57_09990"
     CDS             complement(1308..2021)
                     /locus_tag="VFR57_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEU0259733.1"
                     /translation="MHDIATRLGNTTTGQLLVRSGIEIERVLKSLVDNSTPLSAKLPD
                     VIFLSRLVSLDPIERLVTVDYSDHKPANTALLAMKSVTFMANQYGAQFAFACSKPRHG
                     MHMGQPAIRMSAPAILLAAEPRHNRVRGQVPGEANVECDLRMGLIAFKAKLVDMSTDG
                     RAFLLGDPAIPMCGGTELKRVQIRSNGGEPIEVDIQVEQVLQAVLPNGARATRIGCRI
                     ITDKERLEKLIRFFVIELR"
     gene            complement(2136..3560)
                     /locus_tag="VFR57_09995"
     CDS             complement(2136..3560)
                     /locus_tag="VFR57_09995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0259734.1"
                     /translation="MIDLVIRDATVIDGTRAPRFAGDIAIRDGRIAAIGRVDEKANRE
                     INARGKIAAPGFIDAHTHDDRLMLSSPDMAPKVSQGVTTVVAGNCGISLAPAPKGMRA
                     PVAVPLDLLDDEGGWFRYKTFKEYVDALRSQPAATNCALLVGHSMLRVQTMDSTSRPA
                     TREEIKNMRAMVEEAMEAGALGLSTGLYYEPANAAPTEEVIELCRPLAERNGIYCTHM
                     RNEGEHILDSLEETFRVGREANVPVVISHHKVAGKPNHGRSAETLPVIAKAMREQRVA
                     LDCYPYHASSTILSASRVANATKVLVTWSKPHPEAAGMDLEEIKNKLNLSVEDLLPAG
                     AIYFSMDEKDVQRILAFEPTMIGSDGLPHDAAPHPRLWGTFPRVLGHYSRTVGLFPLE
                     TAIHKMTGLTARTFGLTDRGVLAKGYAADIVIFSAEEVDEAASFSKPIQAAKGIDTVL
                     VNGETVWRDGRPSGARPGRVLSRQ"
     gene            complement(3553..4497)
                     /locus_tag="VFR57_10000"
     CDS             complement(3553..4497)
                     /locus_tag="VFR57_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259735.1"
                     /translation="MRLFLLLFFLSWSAYAAYPEKPIRFILPSAAGGSVDVLMRVLAQ
                     QLSTQMGVAFVVENKPGGAFVPGTMDIVRAPPDGYTLGYGNIVSLAINRALLPKLPYD
                     VEKDLTLISNCVQVFNMLAVNNDLPVRSVQELVAYAKKNPGKLTNGSSGNGTTGHLGG
                     ELFKFMTGTDIVHVPYKGSPQAINDLMAGNIQVMFDNVPSIGPHVKAGRVRGLGVSAP
                     KRAAQFPDIPTIAEAVPGYETNSWGGVIGPAKLPKEIVSRLTAEIRKALAAPAVAERY
                     RQLDTEPDGAGPEAFLELVRKETPKWADVVKKSGAKVD"
     gene            complement(4499..5113)
                     /locus_tag="VFR57_10005"
     CDS             complement(4499..5113)
                     /locus_tag="VFR57_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0259736.1"
                     /translation="MLTLFYAPGACSMAPHIVLEEGGEKYEKKRMDLSKGEQKTPEYM
                     KIHPLGRVPALKLDDGSPLTENTAILPFLGKRFGLWPTDPVKEAKALSLIGFFAASVH
                     PANGHVGRPERYTEDKSAFPGIQAMGRKTYFEYMKQIDAMYGQREWLSDKYSVLDPYA
                     FIFYVWGVRREYPMAELKNYGVFKDRMVKRPAVQRVMADEGIKL"
     gene            5230..6332
                     /locus_tag="VFR57_10010"
                     /pseudo
     CDS             5230..6332
                     /locus_tag="VFR57_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            6570..6884
                     /locus_tag="VFR57_10015"
     CDS             6570..6884
                     /locus_tag="VFR57_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259737.1"
                     /translation="MFLERLTAREVDVLRQLSRGLAYAQAARELRMSGGAAWTDSLKE
                     RRPTMLLFVPQGYVALVYENGVLRAVVEAGVGFVPSAGRNLQVQYVDLKVQQEEVLAT
                     LH"
     gene            6985..8076
                     /locus_tag="VFR57_10020"
     CDS             6985..8076
                     /locus_tag="VFR57_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259738.1"
                     /translation="MNVLFLLHGVGRHVEGWSRDPVAALQAAMKLYPECFEAGKSLDQ
                     YLTVVEIKYDDIFDTVLDRWADLAAALPAPTGGFNWTSKVTQLLKKAGDERNIFARYG
                     GDVLLYCGFALVARAARLRINSLIASTILEENEAALSDRRSPPKFALAAHSLGTTVAQ
                     DALYQLASGRWISDEQQLAADPNFVSALASTAPPGDLAVNLHGLFLVSDTSPVLHQSG
                     YYSELERNGAYDCHAVWSINHELDPISVVGGAFEPGPWRPDKKNVRVGHFHSENIHDL
                     AHYLAHPAVHCEIFRLLIPQFSGACYQKAQTLAEAPLWKGFGGQLAGWLTQERSKLKR
                     KLRTAARRDRQPMKLRDATEKYYKAIGLL"
     gene            8076..8978
                     /locus_tag="VFR57_10025"
     CDS             8076..8978
                     /locus_tag="VFR57_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259739.1"
                     /translation="MRRAAFILFLFATSAGAAELRGDAIFLCDALRQPSIKSSEATAY
                     GKLADALAAAVGNERTRPEVARFLAVLRNPQGRSFDVAPEGNTEKIFRSVPAETITIS
                     CAPSVPQYLVEVATAALLAERIRIEHELPVMKTRAAILEKASRDAEDLLKNGLAMWPW
                     ELWLNGLRLSDKDADPLFRTQWVVLRPSAGIEIDLRSQSSADLQASVMLEPLGFVRYR
                     GDSYAHWWGASLVVTSSTRRGAGIGALLRWDNYVIGLTRHKGEGSEPDSTFLMVGVDL
                     YDLVNKKRTELKDWDAFARLRSAP"
     gene            complement(8935..10419)
                     /locus_tag="VFR57_10030"
     CDS             complement(8935..10419)
                     /locus_tag="VFR57_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0259740.1"
                     /translation="MFENLALGFAVAFSLENLAYCFAGVALGTLIGVLPGITPLVAIG
                     MLFPLTFTLPPIPSLIMLAGIYYGAQYGGSTTAILVNLPGETGAAVTCIDGYQMARQG
                     RAGPALAVAALSSFFAGCVGTLLIALLSPPLGEWALKFGPWEYCALMLMGLVASAVLA
                     HGDAVKGLAMVALGLLFGIVGTDVNSGWQRYTFDVPSLYDGIGFTVIAVGLFAVAEIA
                     QNLESRELRAAFTGRVPRLMPSRSDIADSAWPAARGTAIGAVFGVLPGTGPALCSFAA
                     YMLEKKLAASPERFGHGAIEGVAAPEAANNAAAQTGFIPTLTLGIPGSAVMTLMVGAM
                     MMHGLAPGPNAITEKPELFWGIVASMWIGNLMLVVLNLPLVGLWVRLLNVPYRWLFPS
                     IVMFCCIGNYSVNNNPVDVYLCAALGLLGYVLLKLECEPAPLLLGYVLGPLLEEHLRR
                     AMILGRGDMTPFFTRPISLAFMLGTALVLVLMVRSAASQKRPSP"
     gene            complement(10412..>10692)
                     /locus_tag="VFR57_10035"
     CDS             complement(10412..>10692)
                     /locus_tag="VFR57_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0259741.1"
                     /translation="GLGWSKTEAPVHWDLRPVGCIVGAMVAFGFVLPRFGLIAALVAM
                     FLIAAFAGRDLRWREVVVLTVVMTALAVGVFVVLLKLPFQLVPGFYLV"
BASE COUNT         1831 a         3560 c         3477 g         1824 t
ORIGIN      
        1 tgtccggacg cttcgccgtt gaccgtacgg cgcgtgcaag tccccgccca atatcgatct
       61 gcccggcctg aacctggcta ccgccaacgc ctgacgtagc gctatccagc ccgctgcccc
      121 cgcccgctac gttcgcggtc agcgttccgg cattcgtcgc tgtaattact gcggcgctaa
      181 cgttgccgct gtattgcagc ggcggagtgc cgccaccacc gccgccgcca ccaccaccgc
      241 caccgccgca cgaaacaagc atcgcgagcg cgatgcccgt ggccgcgctt ctcaccttgt
      301 tgtaagcgct catgctccct ccctaccgga acgatgctat cccttttctc gcctccggta
      361 atccccaaac caagctatgc gccccggtct ttcatcatct tctcggcgcg cttctcgagc
      421 tcgcgcggcg ggacgtcctc gacgtgggtg gcgatgtgcc agacgtggcc gaaggggtcc
      481 tggacgctgc ctgtgcggtc gccgtagaac ttgtcctcga gcggttgcac gaccttggcg
      541 ccggcctcaa cggcgcgctc gacgaccttg tccgcgttcg gcatgtagat atgcagatgc
      601 acggcgctgc cgccgcggct cagtgggccg aggaaatcca tctcgcggta ttcgtcggcc
      661 agcatcacgc gcgagtcgcc gatgcggagt tccgcgtggc cgatttttcc gcccggcccg
      721 ggaacgcgca gcagttcttt cgcgccgaag gctttcttgt agaacgcgat ggcctgcgcg
      781 gcatcggcgc aggcgagata gggggtgacg gtgtgatatc cgtccggaac gggcttcact
      841 tttttcatcg gtcgggcctt tttcactcgc aagccatgat tgagccacat tgccggcgca
      901 cgctgggcac tacaggagcc tgagcggcgc tcgggacgct atcctccctc cccatgcgac
      961 tccgggcgcc gctcctcttg tgcttgctgc tggcggcttg caccgtggcg tgggacgagc
     1021 gccacctcga ggcgacgatc gtacggcctt cgaacttcaa agcgggctca ggggtgatcc
     1081 agtcggtggg cgtgctgcgc ggccagcgtg aggcgggaac gggagtcgat gcggacggga
     1141 agcacccgga ccgcaatctc tatcggctgc tcatccacat ggacgcgggc ggtgttcaga
     1201 ccgtggacat cgattccagc cgcttcctgg ccggcgaagc ggtggagctc acgaacgacg
     1261 ggcgcgttgt gcgcgtgagc ggcacgacgc taattcagaa gtagccgcta ccgcagctcg
     1321 atcacgaaga agcggatcag cttctccagg cgttccttgt cggtgatgat gcggcagccg
     1381 atgcgcgtgg cgcgcgcgcc gttcggcagc acggcctgta gcacctgctc gacttgaatg
     1441 tcgacctcga tgggctcgcc gccgttgctg cggatctgca cgcgcttgag ctcggtgccg
     1501 ccgcacatgg ggatcgccgg atcgccgagc aggaacgctc tgccgtccgt gctcatgtcg
     1561 acgagcttgg ccttgaacgc gatgaggccc atgcgcaggt cgcactcgac gttcgcctcg
     1621 ccgggcacct ggccgcgcac ccggttgtgg cgcggctccg cggcgagcag gatggccggc
     1681 gcgctcatcc ggatcgccgg ctgtcccatg tgcatgccgt gccgcggttt ggagcaggcg
     1741 aaggcgaact gcgcgccgta ctggttcgcc atgaaggtca ccgatttcat cgccaacagc
     1801 gccgtattgg cgggcttgtg gtcgctgtag tcgacggtaa cgaggcgctc gatgggatcg
     1861 agcgagacca gcctggagag aaaaataacg tcgggcagct tggccgaaag cggagtgctg
     1921 ttatcgacca acgacttcag gacccgctcg atttcgatcc cggagcgcac gagcagctgc
     1981 cccgtggtgg tgtttcctag ccgcgtggca atgtcatgca ttgtcatgat gctgcagaac
     2041 atgcaacacg gcagcaatga aagattgcgc agcgcgctaa agattgcgca gctcgcgata
     2101 gcgcagctcg cgcgcccgtt tgcgggcggc gtgacttatt gtcggctcag gactcggccc
     2161 ggccgcgcgc cgctcggcct gccatcgcgc cagactgtct cgccattgac aagcacggtg
     2221 tcgatgccct tcgcggcctg gatcggcttg gagaagctcg ccgcttcgtc gacctcctcc
     2281 gcgctgaaga tgacgatgtc cgccgcgtag cctttcgcga gcacgccgcg gtcggtgagt
     2341 ccgaacgtgc gcgccgtgag gccggtcatc ttgtgaatgg ccgtctcaag gggaaaaagt
     2401 cccaccgtcc tcgagtaatg cccgagcacg cgcgggaacg tgccccagag gcgcggatgc
     2461 ggcgccgcgt cgtgcggcag gccgtcggag ccgatcatcg ttggctcgaa cgccaaaatg
     2521 cgctgcacat ccttctcgtc catcgagaag tagatcgccc ccgccggcag caggtcctcg
     2581 accgagaggt tcagcttgtt cttgatctcc tcgaggtcca tgcccgcagc ctcgggatgc
     2641 ggcttcgacc acgtgacgag caccttcgtc gcattcgcca cgcggctcgc cgaaaggatg
     2701 gtcgaagacg cgtggtaggg atagcagtcg agggcgacgc gctgctcgcg catcgctttg
     2761 gcgatcaccg gcagcgtctc ggccgagcga ccgtggttcg gcttgcccgc gaccttgtgg
     2821 tgcgagatga ccacgggcac gttcgcttcg cgcccgaccc ggaaggtctc ctcgagcgaa
     2881 tcgagaatgt gctcgccctc gttacgcatg tgcgtgcaat aaatgccatt tcgctccgcg
     2941 agcggccgac agagctcgat cacctcttcg gtcggcgcgg cattcgccgg ctcgtagtag
     3001 aggccggtcg aaagccccag cgcacccgcc tccatcgctt cttccaccat cgcgcgcatg
     3061 ttcttgattt cctcgcgggt cgccgggcgc gaggtgctgt ccatggtctg cacgcgcagc
     3121 atggaatggc cgacgagcag agcgcagttg gtggcggcgg gctgcgatct cagcgcatcg
     3181 acgtactcct tgaacgtctt gtagcggaac cagccgccct cgtcgtccag caggtcgagc
     3241 ggcaccgcga ctggggcgcg catgcccttc ggcgcgggcg cgagcgagat gccgcagttc
     3301 ccggcgacca ccgtggtcac gccctggctc accttcggcg ccatgtcggg cgacgaaagc
     3361 atcaggcgat cgtcgtgcgt gtgcgcgtcg atgaagcccg gagcggcgat cttgccgcgg
     3421 gcgttgattt ctctgttcgc cttttcgtcg acgcggccga tggcggcgat cctgccgtcg
     3481 cggatcgcga tgtcgcccgc gaaccgcggc gcccgcgtgc cgtcgatgac ggtcgcgtcg
     3541 cgaatgacga ggtcaatcaa ctttggcccc ggacttcttc acgacgtcgg cccatttcgg
     3601 cgtttccttg cgcacgagct cgaggaaggc ctccggcccc gctccgtcgg gctcggtgtc
     3661 gagctgccga tagcgctccg cgaccgcagg agcggcgagc gccttgcgga tctcggcggt
     3721 cagcctggag acgatctcct tgggaagctt cgccggcccg atcacgccgc cccaggagtt
     3781 cgtctcgtag ccggggaccg cctcggcgat cgtcggaata tccggaaact gcgccgcccg
     3841 cttcggcgcg gaaacgccca gcccgcgcac ccgaccggct ttgacgtggg gcccgatcga
     3901 cggaacgttg tcgaacatca cctggatatt gccggccatg aggtcgttga tcgcctgcgg
     3961 gctgcccttg tagggcacgt ggacgatgtc ggttccggtc atgaacttaa agagctcgcc
     4021 gcccagatgc ccggtagtgc cgttgcccga cgagccgttg gtgagcttgc cagggttctt
     4081 cttcgcgtag gcgacgagct cctggaccga gcgcaccgga agatcgttgt tcaccgccag
     4141 catgttgaac acctgcacgc agttggagat gagcgtgagg tccttctcca cgtcgtaggg
     4201 cagcttcgga agcagggcgc ggttgatcgc gagcgacacg atgttgccgt agccgagcgt
     4261 gtagccgtcg ggcggcgcgc ggacgatatc catggtcccc ggcacgaagg caccgccggg
     4321 cttgttctcg acgacgaacg ccacgcccat ttgggtggag agctgctggg cgagaacgcg
     4381 catcagcacg tcgaccgagc cgcccgcggc cgagggcagg ataaagcgga tcggcttctc
     4441 ggggtaagcg gcgtacgcgc tccacgataa gaaaaatagg aggaggaaaa ggcgcatatc
     4501 agagcttgat gccttcatcg gccatcacgc gctgcacggc cgggcgcttc accatgcggt
     4561 ccttgaagac gccgtagttc ttgagctccg ccatcgggta ttcgcgccgc acgccccaca
     4621 cgtagaagat gaaagcgtag ggatcgagca ccgaatactt gtccgagagc cactcacgct
     4681 gcccgtacat ggcgtcgatc tgcttcatgt actcgaaata cgtcttgcgc cccatcgcct
     4741 ggatgccggg aaaggcgctc ttgtcctcgg tgtagcgctc cgggcggccg acgtgcccgt
     4801 tcgcgggatg cacgctcgcg gcgaaaaagc cgatgagcga gagcgccttc gcttccttca
     4861 ccggatcggt cggccacagg ccgaagcgct tgccgagaaa cggcaggatc gcggtgttct
     4921 cggtaagcgg gctgccgtcg tcgagcttga gcgccggcac gcggccgagc gggtggatct
     4981 tcatgtactc gggcgtcttc tgctcgccct tcgagaggtc catgcgcttc ttctcgtact
     5041 tctcgccgcc ctcctccagc acgatgtggg gcgccatgga acaggcgccg ggcgcataga
     5101 acagcgtgag cattgcggtc tcctcgcggt ggggtggaag cgtgatcata attcggcatg
     5161 cgagacctgc cgctcaccgg tttgaaagtc gtcgacctca cccgcgtgct cgccggaccg
     5221 ttctgcgcga tgcttctcgg cgacatgggc gccgacgtca tcaaggtcga ggagcccaac
     5281 gccggcgacg acgcgcgtgc ctggggaccg ttcgtcgcgg atggctggag cgcttacttc
     5341 ctcggcgtga accgcagcaa gcgcagtctg gcgctcgatc tcaaaagcgc ggaaggcgcc
     5401 gatgcgctgc ggcgcctgct gcaaagcgcc gacgtgtttc tcgagaactt caggcccggt
     5461 agcctggaca agctggattt gggcgtcgca gaggttcgcc gcctgaatcc gcggatcgtg
     5521 cactgctcga tcagcgggta cggcaagaac gggccgaagc gtcacctctc ggggtacgac
     5581 cggtcgcgca ggccgagtct gggttcatgg acatcaccgg ccaggccgac ggcccgccga
     5641 tgcgcaccgg catcgccatg accgattact tggcgggact ctacgcgttc ggcggcatcc
     5701 tcctcgcgct tcgcgatcgc gatcgcacca gcctcggcca gcacgtcgac atcgcgcttt
     5761 tcgattcggt gctctcgaca ctctcgatgc cggccggcat tctccacgcc acgggccgca
     5821 gcccgaggcg cagcggcaac gaccatccgt tgctcgcgcc ttacgagacg ctgcgcggca
     5881 aagacgggat ggtgatgatc gccgcggcca atccccgcct ctggaagcag ctttgcgaag
     5941 cggtcggtct cggccacctc gtcgacgatc ctcgctttcg caccaacacc gatcgggtga
     6001 agaaccgggc agagttgaag gtcgagctcg agcgcgcgtt cgcccagttc agcgtcgatg
     6061 agcttgcacg ccgcctggag gcggcctcag taccgtgcgg ccgggtgcgc tcggtcgccg
     6121 aagccctcgc cgacccgcag atccagccgc gccagatgct cctcgacttc gacgatcccg
     6181 gcctcgccgg cttccgcgtc ctcggcaacc cgatcaagct ctccaaccac cccagcgacg
     6241 gaaaagcgcg ccgtcccccg cgcctcggcg agcacacgca agaaatcctg catgagctaa
     6301 tgagcaaccg taattcgggg tcagggtcct aattcggtca accgcaaagc gccgcaacgc
     6361 gtttgttggg tattgacttc cgatgcggcg cgcgagcaga atgcgcgccc tcttggagcg
     6421 aggacgcaat gcagccgttg aaaacgcaca actgcctacg agatcgccgg atatccggcg
     6481 aggacgctgc gcgtccggac gcggaggcct gctagcgcga tagcgcctgc tctcccacag
     6541 cccggaccgc gccagcgctc cgggcttttt tgtttctgga acgcctgacc gcacgtgaag
     6601 tcgacgtgct gcgccagctc tcgcgcgggc tcgcctacgc gcaagccgcg cgcgagctcc
     6661 gcatgtcggg cggtgcggcc tggactgatt ctttgaaaga aaggaggccg acgatgcttc
     6721 tgttcgttcc gcaaggctat gtcgccctgg tctacgaaaa cggcgtgctg cgcgccgtgg
     6781 tcgaggccgg cgtcggcttc gtgccgagcg ctgggcgtaa cctgcaggtc cagtacgtcg
     6841 acctgaaggt ccaacaagaa gaagttctcg cgaccctgca ctagggcgtg actcgagggc
     6901 cggagacctc cggcccttct ttatctatgc gtctcgttat tttcaccctt gcgaacgcgc
     6961 gagctccgtg tcgacaatgc gcgcgtgaac gtcctgtttc tcttgcatgg ggtcggacgg
     7021 cacgtcgagg gatggagccg cgatccggtc gccgcgctcc aggctgcgat gaagctctat
     7081 ccggagtgct tcgaagccgg taagtcgctc gaccagtacc tcaccgtcgt cgagatcaag
     7141 tacgacgaca tctttgacac ggtgctcgac cgctgggccg acctcgccgc ggctctgccg
     7201 gcgccgacgg gcgggttcaa ctggacgagc aaggtcacgc agctcctcaa gaaggctggc
     7261 gacgagcgca atatcttcgc gcgctacggc ggtgatgtgc ttctctactg cggctttgcg
     7321 ctggtcgcgc gcgccgcgcg gctcaggatc aattcgctca tcgcttccac gattctcgag
     7381 gagaacgaag ccgccctgag cgatcggcgc agcccgccga agttcgcgct cgcggcgcat
     7441 tcccttggca ccacggtcgc gcaggacgcg ctgtatcagc tcgccagcgg ccgctggatc
     7501 tccgatgaac agcagcttgc cgcggacccc aacttcgtct ccgcgctagc ttcaaccgcg
     7561 ccgccgggcg acctcgcggt caacctgcac ggtctcttcc tggtgagcga cacctcgccg
     7621 gtcctgcacc aatccggcta ttactcggag ctagagcgca acggcgccta cgattgccac
     7681 gccgtgtggt ccatcaacca cgagctcgac ccgatcagcg tcgtcggcgg cgcgttcgag
     7741 cccggcccgt ggcggcccga caagaagaat gtgcgcgtcg gccatttcca cagcgagaac
     7801 atccacgatc tagcgcatta cctcgcgcat ccggcggtgc actgcgagat cttccggctg
     7861 ctcattccgc agttcagcgg cgcctgctac cagaaggcgc agacgctcgc tgaggcgcca
     7921 ctgtggaagg gattcggcgg gcagctcgcc gggtggctca cgcaggagcg cagcaagctc
     7981 aagaggaagc tgcgcaccgc cgcccggcgc gatcggcagc cgatgaagct gcgcgatgcg
     8041 acggaaaaat actacaaggc gatcggcctc ctgtgatgcg gcgcgccgca ttcattctct
     8101 tcctctttgc gaccagcgcc ggcgcggccg agctgcgcgg cgacgcgatc ttcctctgcg
     8161 atgcgttgcg ccagccgtca atcaagagct cggaggcaac cgcctacggc aagctcgccg
     8221 acgcgctggc cgccgccgtg ggaaacgagc gcaccagacc ggaagtcgcg cgcttcctcg
     8281 cggtgctgcg caacccgcag ggccgctcct tcgacgtcgc gccggagggc aataccgaga
     8341 agatttttcg cagcgtgccg gccgagacga tcaccatttc ctgcgcgcca tcggttcccc
     8401 aatacctcgt cgaggtcgca accgccgcgc ttcttgccga gcgcatccgc atcgagcacg
     8461 agctgcccgt catgaagaca cgtgcggcga ttcttgagaa agcctcgcgc gatgccgagg
     8521 acctgctcaa gaacggcctt gccatgtggc catgggagct gtggctgaac gggctgcgcc
     8581 tttcggacaa ggacgccgac ccgctcttcc gcacgcagtg ggtggtgttg cgtccgagcg
     8641 ccggcatcga gatcgacctg cgcagccagt cttccgccga cctgcaggca agcgtgatgc
     8701 tcgagccgct cggcttcgtg cgctatcgcg gcgattcgta cgcgcactgg tggggcgcgt
     8761 cgctggtcgt cacgtcgagc acccgtcgcg gcgcgggtat cggcgcgctg ctgcgctggg
     8821 acaactacgt gatcggcctc acgcgccaca agggggaagg aagcgagccc gacagcacct
     8881 tcctgatggt cggcgtggac ctctacgacc tcgtgaacaa gaaacgcacc gagctcaagg
     8941 actgggacgc ttttgcgagg ctgcggagcg caccatgagc acgaggacga gcgccgttcc
     9001 gagcatgaac gcgaggctga tcggccgagt gaagaagggc gtcatgtcac cgcggccgag
     9061 aatcatcgcg cggcggaggt gttcttccag aagcggaccg agcacgtagc cgagaagcag
     9121 cggcgccggc tcgcattcca atttcaggag gacatacccc agcaggccga gcgcggcaca
     9181 caggtacacg tcgaccggat tgttgttcac gctgtagttg ccgatgcagc agaacatgac
     9241 gatcgacgga aagagccagc ggtacggcac attcaagagg cgcacccaga ggcccacgag
     9301 cggcaggttc agcaccacga gcatgaggtt gccgatccac atgctcgcca cgattcccca
     9361 gaagagttcg ggcttctcgg tgatggcgtt cggtccgggc gcgaggccgt gcatcatcat
     9421 cgcgcccacc ataagcgtca tcaccgcgct tcccgggatg ccgagggtga gcgtgggaat
     9481 gaacccggtc tgcgccgcgg cgttgttcgc ggcttccggc gccgccacgc cctcgatggc
     9541 gccgtggccg aagcgctcag ggctcgccgc aagcttcttc tcgagcatgt aggcggcgaa
     9601 agagcaaagc gcaggtccgg ttcccggcag tacgccgaac accgcgccga tcgcggtgcc
     9661 gcgcgccgcc ggccacgccg agtcggcgat gtcggaacgg cttggcatga gccgcggcac
     9721 gcgacccgta aacgccgcgc ggagctcgcg cgattccagg ttctgcgcga tctcggccac
     9781 ggcgaagaga cccaccgcga tgaccgtgaa gccgatgccg tcgtagagcg agggcacgtc
     9841 aaacgtgtag cgctgccagc ccgagttgac gtcggtaccg acgatgccga aaagcaggcc
     9901 gagcgcgacc atggcgagcc ccttaactgc gtcgccgtgc gcgagcaccg ccgaggcgac
     9961 gaggcccata agcatcagcg cgcagtactc ccacgggcca aacttgagcg cccactcgcc
    10021 gagcggcggg ctcagtaggg caatcaatag cgttccgaca cagccggcaa agaacgagga
    10081 caaagccgcg acggcgagtg ccggccccgc ccttccctgc cgcgccattt gatagccgtc
    10141 gatgcaggtc accgcggcac cggtctcgcc cggcaggttt acgaggatcg cagtcgtcga
    10201 gccgccgtac tgcgcgccgt agtaaatgcc ggcgagcatg atgagcgagg gaattggcgg
    10261 cagggtgaac gttagcggga acagcatgcc gatcgcgacg agcggcgtga tgccgggcag
    10321 cacgccgatc agcgtgccga gcgcgacgcc cgcgaagcag tacgcgaggt tctcgagcga
    10381 gaacgccacg gcgaagccga gcgccaggtt ctcaaacaag gtagaacccg ggcacgagct
    10441 ggaacggcag cttgagaagg acgacaaaca cgcccacggc gagcgccgtc atcaccacgg
    10501 tcagaacgac cacttcgcgc cagcgcaggt cgcggccggc gaacgccgcg atcaggaaca
    10561 tcgcgaccag cgcggcgatg agcccgaacc ggggcagcac gaacccgaac gcgaccatgg
    10621 cgccgacgat gcagccgacc ggccgcaaat cccaatgcac cggcgcctcc gtcttactcc
    10681 agcccaggcc gc
//
LOCUS       DASTPD010000300         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_774712, whole genome shotgun sequence.
ACCESSION   DASTPD010000300 DASTPD010000000
VERSION     DASTPD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774712"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFR57_10040"
     CDS             <1..211
                     /locus_tag="VFR57_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259742.1"
                     /translation="DGRSLRHHTVAVRGTAENPMTRAEVDAKSYDLMAPALGKARARK
                     LCETVWNLETVSDVRRLRPLLQEKS"
     gene            208..603
                     /locus_tag="VFR57_10045"
     CDS             208..603
                     /locus_tag="VFR57_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259743.1"
                     /translation="MNRIVHLALKVEDLERTTEFYQKVFGFVEMNTEKVRDHTSRHLS
                     DGVLDIALIKYDAGAQSAESKAAGEGPCIHHFAVEVEDVDKATEQVKSYGCEIISDPG
                     VIPVKFRAPGGTVCELVPKGRYKKPKAKP"
     gene            600..1031
                     /locus_tag="VFR57_10050"
     CDS             600..1031
                     /locus_tag="VFR57_10050"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0259744.1"
                     /translation="MKRYGGDFWSGLALGGLGAYIIVHSARWEYLGSDGPGPGFFPLW
                     YGIAIAALSLALVISSLRTKDSESVEWAGASRALAAWAAFATCVGLLKVVGFFVSLAA
                     LTFFIVAVMYRRPLRVAVAVTAAIAVAFYVVFPLALGVELP"
     gene            1035..2537
                     /locus_tag="VFR57_10055"
     CDS             1035..2537
                     /locus_tag="VFR57_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0259745.1"
                     /translation="MEVLSGLMQGFAVALQPVNLLWCLVGVALGTVVGILPGLGPPAT
                     VAMLLPLTMLMNPASAMIMLAGIYYGAKYGGSTTSILLNVPGESASVVTCLDGYQMAR
                     KGRAGAALGIAAIASFIAGTVGVLGLMLVAPPLARMALAFSSPEYFGLMALGLAMVVL
                     LAGRSMVKALLAMLVGLWLAGIGTDLFTQTSRFTFGQLELLSGIDFVVVAIGVFAVGE
                     VLANMGRRDGTESLPVPRGWRNLLPTGQDLKDSRFAFANGSIVGFLIGVLPGAGSTIA
                     SFISYGLEKAVSRRPQEFGTGVVEGVAAPEGANNAETGGALIPMLTLGIPGSSTTAIL
                     LAALVLWGFKPGPLFIPENPTLFWGLVASMYMGNLMLLVLNLPLVPLFAQALRAPIYV
                     LFPVILGISLVGVYSASGSLFDVWLLAAFGLGGYLMRKLDYPSAPLILGFVLGAPLER
                     ALRQSLMMSDGSLSILVSRPVPALMLSLAVLILLIPLIGKVNSWRLRALE"
     gene            2561..>3180
                     /locus_tag="VFR57_10060"
     CDS             2561..>3180
                     /locus_tag="VFR57_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0259746.1"
                     /translation="MGKKLVLAAVLAVTNTAFAQGFTPTRPVETVVHTGPGGGSDVLA
                     RAVALMMEKEKLLPVRMQVVNKTGGGGAVAAAYLAEKKGDTHTIGFFTGLWLTNPLTS
                     AEAKVTLKDLTPVARLVLEPAVIAVKADAPYKTLADFVNAAKQKPGELKQSGGSVTSR
                     DNVVRQQLQKVSGARWQFISFPGGGERIAALLGGHVNMMVIEPAEAG"
BASE COUNT          483 a         1005 c         1081 g          611 t
ORIGIN      
        1 cgacggccgc agcttgcgcc atcacacagt ggcagtgcgc ggcaccgcgg aaaaccccat
       61 gacgcgcgcc gaagtcgacg ccaagagcta cgatctgatg gcgccggcgc tcggtaaggc
      121 tcgcgcgcgc aagctgtgcg aaacggtctg gaatctggag acggtttccg acgttcgcag
      181 gctgagacct ttgctacagg agaaatcatg aatcgcatcg tgcatctcgc gctgaaggtc
      241 gaggacctcg agcgcactac cgagttctat cagaaggttt tcggcttcgt ggagatgaac
      301 accgagaagg tgcgcgacca tacctcacgc caccttagcg acggagtgct cgacatcgcg
      361 ctcatcaaat acgacgccgg tgcgcagtcg gcagaatcca aggcagcggg cgaggggcct
      421 tgcatccacc acttcgcggt cgaggtcgaa gacgtggaca aggcgaccga gcaggtgaag
      481 agctatggct gcgagatcat cagcgacccc ggcgtaatcc cggtgaaatt tcgcgcccct
      541 ggcggaacgg tgtgtgagct agtgccgaaa ggccgctaca agaagcccaa agcgaagcca
      601 tgaagcgcta cggcggtgat ttctggtccg gcctcgcgct cggcgggctc ggcgcgtaca
      661 tcattgtgca ttccgcgcgc tgggaatacc ttggcagcga tggccccggg ccgggattct
      721 ttccgctgtg gtacggcatc gccatcgcgg cgctgtcgct ggcgctggtg atttcgagtc
      781 tcaggaccaa agactccgag agcgtcgaat gggctggcgc ctcgcgcgcg cttgccgcgt
      841 gggcggcgtt cgccacctgc gtcggcctgc tcaaggtcgt ggggttcttt gtgtcgctcg
      901 ctgcgctgac ctttttcatc gtcgccgtca tgtaccgccg gccgctgaga gtggctgtcg
      961 cggtgacggc tgcgatcgca gttgcgttct acgtagtgtt cccgttggcg ctcggcgtgg
     1021 agctccctta ggtcatggag gtgctgtccg gcctgatgca gggcttcgcg gtggcgctgc
     1081 agccggtcaa cctgctgtgg tgcctggtcg gcgtcgcgct cggcacggtg gtcggcatcc
     1141 tgcccggcct cggccctccc gcgaccgtcg cgatgctgct gccgctcacc atgctcatga
     1201 atccggcgag cgcgatgatc atgctcgcgg gtatctacta cggtgcgaag tacggaggct
     1261 cgaccacctc catcctgctc aacgtgccag gggagtcggc ctcggtggtg acctgcctcg
     1321 acggctacca gatggcgcgg aagggtcggg ccggcgcggc gctcggaatc gcggcgatcg
     1381 cctcgttcat cgcgggcacc gtgggcgttt tgggcctcat gctggtcgcg ccgccgctcg
     1441 cgcgcatggc gctcgcgttc agctcgcccg agtatttcgg cctgatggcg ctcgggctgg
     1501 caatggtggt gctgctcgcc gggcgctcga tggtcaaggc gctgctcgcc atgctggtcg
     1561 gcctctggct cgccggcatc ggcacggatc tcttcacgca gacctcccgc ttcactttcg
     1621 ggcagctgga gctcctttcc ggcatcgatt tcgtagtggt ggccatcggg gtgtttgcgg
     1681 tgggcgaggt gctcgcgaac atgggacggc gcgacggcac cgaatcgctc ccggttccca
     1741 ggggatggcg caacctcctg cctaccgggc aggacctgaa ggactcgcgc ttcgccttcg
     1801 ccaacggctc gatcgtcggc ttcctgatcg gcgtcctccc tggcgccggc tcgaccatcg
     1861 cctcgttcat ctcctacggg ctggagaagg cggtgtctcg ccgcccgcag gagttcggca
     1921 cgggcgtggt cgaaggcgtc gccgcgcccg aaggggcgaa caacgccgag accggaggcg
     1981 cgctcatccc gatgctgacg ctggggatcc ccggctcgtc gaccaccgcg atcctgctcg
     2041 ccgcgctggt gctgtgggga ttcaagccgg ggccgctttt catcccggaa aacccgacgc
     2101 tgttctgggg tctggttgcg agcatgtaca tgggcaacct gatgctgctc gtcctgaacc
     2161 tcccgcttgt gccgcttttc gcccaggccc tgcgcgcccc gatctacgtc ctgttcccgg
     2221 tgatcctcgg aatttcgctg gtcggtgtct acagcgcctc cgggagcctg tttgacgtct
     2281 ggctgctggc tgccttcggg cttggcgggt acctaatgcg caagctcgac tacccgtcgg
     2341 cgccgctgat cctgggcttc gtcctcggag cgccgctcga gcgggcgctg cgtcagtcgc
     2401 tcatgatgtc cgacggcagt ctttcgatcc tggtgtctag gccggtgccg gcgctgatgc
     2461 tatcgttggc tgtgctgatt ctgcttattc cgctgattgg caaagtgaac tcctggcgcc
     2521 tgcgcgcttt ggagtagtca aaaacgagga ggaggacttg atgggcaaga aacttgtgtt
     2581 ggcagctgtt ctcgcggtga caaacacggc gttcgcgcaa gggttcaccc ccacgcggcc
     2641 ggtggagacg gttgtgcaca ctggcccggg cggcggcagc gatgtgctcg cccgcgcggt
     2701 cgcgctcatg atggagaagg aaaagttgct gccggtgcga atgcaggtgg ttaacaagac
     2761 cggcggcggc ggcgcagtgg cggccgcgta cctcgcggag aagaagggtg acacccatac
     2821 gatcggtttc ttcaccggct tgtggctcac caacccgctc acgagcgcgg aggccaaggt
     2881 tacgctcaag gacctcacgc cggtcgcgcg cctggtgctc gagccggcgg tcatcgcagt
     2941 gaaggcggat gcgccctaca agacgctcgc cgatttcgtc aacgccgcca agcagaagcc
     3001 gggcgagctc aagcagtccg gcggctcggt tacgagccgc gacaatgtcg tgcgccagca
     3061 gctgcagaaa gtgagcggcg cacgctggca gttcatctcc ttccccggtg gcggcgagcg
     3121 cattgcggcg ctgctcggcg ggcacgtcaa catgatggtg atcgagcccg ccgaggcggg
//
LOCUS       DASTPD010000301         6226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1590144, whole genome shotgun sequence.
ACCESSION   DASTPD010000301 DASTPD010000000
VERSION     DASTPD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590144"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /gene="rpmI"
                     /locus_tag="VFR57_10065"
     CDS             complement(<1..130)
                     /gene="rpmI"
                     /locus_tag="VFR57_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0259747.1"
                     /translation="MPKMKSKRGAAKRFKVRPGGGIKRSQAYMRHILTKKSTKRKRH"
     gene            complement(210..845)
                     /gene="infC"
                     /locus_tag="VFR57_10070"
     CDS             complement(210..845)
                     /gene="infC"
                     /locus_tag="VFR57_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0259748.1"
                     /translation="MFLTYRRSFSDIALDRSQRINREITAPEVRLVAENGDQLGIKSV
                     AEALRLAEEQNVDLVEIAPLANPPVCKLMDYGKFRYREQKKAHEAKLKQKQIQVKEIK
                     FRPGTDEGDYKIKLGKLIQFLEEGDKAKVTLRFRGREMAHKEFGERLLERVRKDLDTI
                     GVVEQFPRLEGRQMVMVLAPKKKGPAPKPKAKDEKEAKPAKPAAPPKRQAA"
     gene            complement(855..2759)
                     /gene="thrS"
                     /locus_tag="VFR57_10075"
     CDS             complement(855..2759)
                     /gene="thrS"
                     /locus_tag="VFR57_10075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0259749.1"
                     /translation="MPNFRLPDGSSKSLPAPVTVADIAASIGPGLARAALAGKVNGKL
                     VDTRYRIESDADVQIVTERDAEGLEILRHSTAHLLAQAVKELFPEAQVTIGPVIENGF
                     YYDFAYKRPFTPEDLLLIEKKMQEIARKDLPIERRELSRDEAVKYFSSIGEKYKAEII
                     ASIPEKETISLYGQGDWIDLCRGPHVPSTGKLKTFKLMRVAGAYWRGDSRNEMLQRIY
                     GTAWARKEDQDAYLRMLEEAEKRDHRRLGTQLDLFHMQEEAPGLVFWHPKGWTIWQQV
                     EQYMRRVYRDNGYLEVRAPQILDRSLWERTGHWENFRENMFTTSSENRDYAVKPMNCP
                     GHILIYNKGVRSYRDLPLRYGEFGSCHRNEPSGALHGLMRVRGFVQDDGHIFCTEAQI
                     LGECVAYTALLQKVYRDFGFTDIIYKVATRPAKRIGSDEAWDKAELALGTALEKSGVR
                     FSVSPGEGAFYGPKIEYSLKDGIGRVWQCGTMQVDFSMPARLGAEYVAEDNTRRTPVM
                     LHRAIVGSFERFIGILIEHYAGAFPLWLAPHQAVVMTITERQNEYAQSVANRLVNQGF
                     RVILDLRNEKISYKIREHSLQKLPYHVVVGDKEMQAGTVAVRTRSGEDLGAMALEAFT
                     ARLQAEAKAQ"
     gene            2786..3724
                     /locus_tag="VFR57_10080"
     CDS             2786..3724
                     /locus_tag="VFR57_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEU0259750.1"
                     /translation="MRLPLTLAINAYDHVRALRPQAIDLTVLELPIEEIFYRFTRFRE
                     WEASEMSFGKVVALLSQPAPDVVAIPVFPSRVFRHSAIYTSKLGTPKELEGKRVGIPE
                     WAQTAGIYVRGLLQHEYGVDLARIDWVQAGVREPGRVEKVALRLPPGVRITPAPDKTL
                     AGMLASGELDAVISARDPGGSRLFADYAALELEYFKKTRIFPIMHVIVLRREVYERDR
                     WIAMNLFSAFEQAKRAAQARITEIGLSHVPMPWLAEHARRWREIAGDDLWPYGIGANR
                     PTLEAFLQYAHEQGVALRRLRVEELFAPETLERAKI"
     gene            complement(3721..4671)
                     /locus_tag="VFR57_10085"
     CDS             complement(3721..4671)
                     /locus_tag="VFR57_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259751.1"
                     /translation="MTLRALLFILLALPAAAAAQFPSRQITLIVPIPPGGAPDIAARL
                     MGQKLSESLGQPVVVENRTGANGNIASDLVAKAQPDGHTLGLLADSQITINPHLYKMP
                     LDTLRDLTPVATVAANQFVLTVHPSLPVRTFPEFIELARKSNPPLAYASGGNGSQHHL
                     TMEMLKQRAGINLLHVPFKGGAPAATATVAGDTVAMWSGSSNAPQIKAGRLRPLAVSG
                     AQRSPQYPDLPTIGEFYPGFENSIWLGLFGPAGIPESALSRMRTEVRRVLESDDVKQK
                     MNAAGGLDPLVTTPQEFGALIRRDFEKYAKIVKDLGLKLD"
     gene            complement(4703..4779)
                     /locus_tag="VFR57_10090"
     tRNA            complement(4703..4779)
                     /locus_tag="VFR57_10090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4743..4745),aa:Val,seq:gac)
     gene            4824..5273
                     /locus_tag="VFR57_10095"
     CDS             4824..5273
                     /locus_tag="VFR57_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0259752.1"
                     /translation="MDLIVWRHAEAEDGVPDLERRLTAKGRKQAERVAQWLLQRLPAR
                     FAVIASPAARAQQTAEALGVPVKTDRSLAPGATPAAIVKACGWPGYKGAAVVVGHQPD
                     LGRALAKLLGAQAGLSIKKGALWWISNRVRDEKDQVVVRAVVSPDLL"
     gene            complement(5256..>6226)
                     /locus_tag="VFR57_10100"
     CDS             complement(5256..>6226)
                     /locus_tag="VFR57_10100"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU0259753.1"
                     /translation="AELAASQELATLGKEYRDTGWDEAVASSGTARSLENILRENDFA
                     DEGLTREGLERLRSLLIKQERADPDRIPGLRSNRAPVLPGGLAIMIAAFDALGLDSIK
                     VSEGALRHGVLYDLLGRVQHRDMREATVAQFMRRYHVDAAQAERVKALALKIYDGLTP
                     GADREDDPERLMLEWAARLAETGLSIAHAAYHKHSAYVLSNADMPGFSRMEQARLARI
                     VLAHRGKLRKVQDAGFESSDWRLVFALRLASLILRSRTDAKLPFLRVAADPGGFAIDL
                     PQSWLDENPLSADALDAEAGHWKSVDMKLEVSGLSDKKVSVLKQVR"
BASE COUNT         1006 a         2010 c         2017 g         1193 t
ORIGIN      
        1 ggtggcgctt tcgcttggtg ctcttcttcg tgaggatatg gcgcatgtag gcctgcgatc
       61 gcttgatgcc gccgcccggg cgcaccttga agcgcttcgc ggccccgcgt ttggacttca
      121 tcttgggcac gattgccctc ctcttctagc ttgacgccgg gcggtccccg cctatgcggg
      181 gacgcttcaa atgccacggt catcacttgt taagccgctt ggcgcttcgg aggcgctgcg
      241 ggttttgctg gctttgcttc cttctcgtct ttcgccttcg gcttcggcgc cggtcccttt
      301 ttcttggggg cgagcaccat caccatctgc cggccttcga gcctgggaaa ctgctccacc
      361 acgccgatgg tgtccagatc cttgcgcacg cgctcgagca gccgctcgcc gaactccttg
      421 tgcgccatct cgcggccgcg gaagcgcagc gtgaccttcg ccttgtcgcc ctcctcgagg
      481 aactggatga gcttgccgag cttgatcttg tagtcgccct cgtccgtgcc ggggcggaat
      541 ttaatctcct tgacctggat ctgcttctgc ttgagcttcg cctcgtgcgc cttcttctgc
      601 tcgcggtagc ggaacttgcc gtagtccatc agcttgcaca ccggcgggtt cgcaagcggc
      661 gcgatctcga cgaggtctac gttctgctcc tcggcaagcc tcagcgcctc cgccacggat
      721 ttgattccca gctggtcacc gttttccgcc accaggcgca cctccggggc ggtgatttcc
      781 cggttgatgc gctgcgatct gtcgagtgcg atgtctgaaa agctcctgcg gtacgttaaa
      841 aaaatcggcc gtgcttactg cgctttcgcc tcggcttgaa ggcgtgcggt aaaggcctcg
      901 agggccatcg ccccaaggtc ctcgccgctg cgcgtgcgca cggccaccgt cccagcctgc
      961 atctccttgt cgccgaccac cacatggtag ggcagcttct gcaggctatg ctcgcggatc
     1021 ttataagaaa ttttctcgtt ccgcaaatcc aaaattaccc taaagccttg attgacaagc
     1081 cgattcgcga ccgactgcgc gtattcgttc tgtcgctcgg tgatcgtcat caccaccgcc
     1141 tggtgcggcg cgagccacag cggaaaagcg ccggcgtaat gctcgatcag gatgccgatg
     1201 aagcgctcga aggagcccac gatcgcgcgg tgcaacatca ctggcgtgcg ccgcgtgttg
     1261 tcttctgcga cgtattcggc gcccagcctg gccggcatgc tgaagtccac ctgcatcgtg
     1321 ccgcactgcc agacgcgccc gatgccgtcc tttagcgagt actcgatctt cgggccgtag
     1381 aacgcgcctt cgccgggaga gacggaaaac ctgacgccgg acttttccag ggccgttcct
     1441 aaagcaagct cagccttgtc ccacgcttca tcggatccga tgcgcttggc gggccgggtc
     1501 gccaccttgt agatgatgtc ggtaaacccg aagtcgcgat acaccttctg cagcagcgcc
     1561 gtatacgcga cgcactcgcc gaggatctgc gcctctgtgc aaaagatgtg accgtcgtcc
     1621 tgcacgaagc cgcgcacgcg catcagtccg tgcagcgcgc cggagggctc gttgcggtgg
     1681 caggaaccga actcgccgta gcgtagcggc aaatcgcgat agctcctcac gcccttgttg
     1741 tagatgagga tgtggccggg gcagttcatc ggcttcaccg cgtagtcgcg gttctccgac
     1801 gaggtggtaa acatgttctc gcgaaaattc tcccagtgcc cggtgcgctc ccacaggctc
     1861 cggtccagga tctgcggggc gcgcacctcg aggtagccgt tgtcgcggta gacgcggcgc
     1921 atgtattgct cgacctgctg ccagatcgtc cagcccttcg gatgccagaa aacgagcccc
     1981 ggcgcctcct cctgcatgtg gaacagatcg agctgcgtgc caagccgccg gtggtcgcgc
     2041 ttctccgcct cctcaagcat cctgagatac gcgtcctggt cttccttcct cgcccaggcg
     2101 gtgccgtaga tgcgctggag catctcgttg cgggagtcgc cgcgccagta cgctcccgcg
     2161 acgcgcatca gcttgaaggt cttcagcttg ccggtggacg gcacgtgcgg cccgcggcag
     2221 aggtcgatcc agtcgccctg gccgtagagt gagatcgtct ccttctccgg aatcgaggcg
     2281 atgatctcgg ccttgtattt ctctccgatg gaggaaaagt acttgaccgc ctcgtcgcgc
     2341 gacagctcgc gccgctctat cgggaggtcc ttgcgcgcga tctcctgcat tttcttctcg
     2401 atcagcaaca ggtcttccgg agtgaaaggt cgcttgtagg cgaagtcgta gtagaagcca
     2461 ttctcgatca ccggaccgat cgtcacctgc gcttccggga aaagctcttt caccgcctgc
     2521 gcaaggaggt gggcagtcga gtgacgcagg atctcaagac cttcggcatc gcgttcggtg
     2581 acgatctgca cgtctgcatc ggactcgatg cgatagcggg tgtcgaccag cttgccgttc
     2641 actttcccgg caagcgcggc gcgcgcgagt cccggaccga tcgaagcggc gatgtcggct
     2701 acggtgacgg gtgctggcaa cgacttcgac gagccgtcag gcagacggaa attgggcatc
     2761 gcggacgagc gtgcatacta tcacggtgag attgccgctg actcttgcga tcaatgcata
     2821 cgatcacgtg cgcgctctgc gtccgcaggc aatcgatctc accgttctcg agctcccgat
     2881 cgaagagatc ttctaccgct tcacgcgctt ccgtgaatgg gaagcgtccg agatgtcgtt
     2941 tggcaaggtc gtcgccctgc tgtcgcagcc ggcgccggac gtcgtggcga ttccggtgtt
     3001 tccttcgcgc gtcttccgcc actcggcgat ctacacgagc aagctcggaa cgccgaagga
     3061 gctcgaagga aagcgcgttg gaattccgga atgggcgcag accgcgggca tctatgtgcg
     3121 cggcctgctg cagcacgagt acggcgtcga tctcgcccgc atcgactggg tgcaggccgg
     3181 cgtgcgcgag cccggacgag tggaaaaggt ggcgctcagg ctgccgcccg gcgtgcgcat
     3241 cacgccggct ccggacaaga cgctcgccgg aatgctcgcg agcggcgagc tcgacgccgt
     3301 gatctcggcg cgcgacccgg gaggctcgcg cttgtttgcc gactacgcgg cgcttgagct
     3361 ggaatacttc aagaaaaccc gcattttccc catcatgcac gtcatcgtgc tgcggcgcga
     3421 ggtctatgag cgcgatcgct ggatcgcgat gaacctcttc agcgcattcg agcaggcgaa
     3481 gcgcgcggcc caggcgcgca tcaccgagat cggcctttcg cacgtcccca tgccatggct
     3541 cgcggagcat gcgcggcgct ggcgcgagat cgctggcgat gatctgtggc cctatggcat
     3601 cggggcgaac cggccgacgc tggaagcgtt tcttcaatac gcccacgagc agggcgtcgc
     3661 gctgcgccgg ctgcgagtgg aggagctgtt cgcgcccgag acgctggagc gcgcgaagat
     3721 ctagtcgagc ttgaggccga ggtccttgac gatcttggcg tacttttcaa agtcgcgccg
     3781 gatcagagcg ccaaactcct gcggcgtggt gacaagagga tcgaggccgc ctgcggcatt
     3841 cattttctgc ttgacgtcgt cggattcgag cacgcgcctg acctcggtgc gcatcctcga
     3901 caaagcactc tccgggatgc cggccgggcc gaacagtccg agccagatcg agttctcgaa
     3961 cccgggatag aactcgccga tcgtcggcag gtccgggtac tgcggcgagc gctgcgcgcc
     4021 ggacaccgcg agcggccgca gccgaccggc cttgatctgc ggcgcgttcg agctgcccga
     4081 ccacatcgcg acagtgtcgc ccgcgaccgt ggcggtcgcc gcaggcgcgc cgcccttgaa
     4141 agggacgtga agcaggttga tgccggcgcg ttgcttcagc atctccatcg tgagatggtg
     4201 ctggctgccg ttgccgcccg aagcgtaggc gagcggtgga ttgctcttgc gtgccaactc
     4261 gatgaactcc gggaaggtcc tgaccggaag cgagggatgc accgtcagca cgaactggtt
     4321 ggccgcgacc gtcgccaccg gcgtgagatc gcgcagcgtg tcgagcggca tcttgtaaag
     4381 atgcgggttg atcgtgatct ggctgtccgc gaggagcccg agggtgtggc cgtcgggctg
     4441 cgccttcgcc accaggtcgg aggcgatgtt gccgttcgcg ccggtccggt tctcgaccac
     4501 caccggttgg ccgaggcttt cggagagctt ctggcccatc agccgcgccg cgatgtcggg
     4561 cgcgccgcct ggcgggatgg gcacgatgag ggtgatctgc cgcgatggaa actgcgcagc
     4621 tgccgcagcc ggcaaggcga gaaggatgaa gagaagcgcg cgcagggtca tgtctctctg
     4681 ctccctatca tcccggttat cctggtaggc gcgattggac tcgaaccaac gacccctacc
     4741 atgtcaaggt agtgctctaa ccagctgagc tacgcgccta gcggtcataa ttttagcgct
     4801 catttacctt agactcgccc ggcgtggatc tgatcgtgtg gcgacacgcc gaggccgagg
     4861 acggcgtgcc ggatctcgag cggcggctca ctgcaaaggg acgaaagcag gccgagcgcg
     4921 tcgcgcagtg gctgctgcag cggctgccgg cgcgcttcgc cgtaattgcg agcccggcgg
     4981 cgcgcgccca gcagaccgcg gaggcgctcg gcgttccggt gaagaccgac cgctcgctcg
     5041 cgccgggcgc aacaccggcg gcgatcgtca aggcctgcgg ctggccaggc tacaagggtg
     5101 cggcggtggt ggtcggtcac cagcccgacc tcggccgcgc gcttgccaag ctgcttggcg
     5161 cgcaggcggg cctcagcatc aagaagggcg ccctgtggtg gatcagcaac cgtgtgcgcg
     5221 acgagaagga tcaggtggta gtccgcgccg tcgtgtcacc ggacctgctt tagtacagaa
     5281 accttcttgt cgctcaagcc gctcacctcg agcttcatgt cgaccgattt ccagtggccg
     5341 gcttcggcgt cgagcgcgtc ggcggaaagc gggttctcgt cgagccacga ctggggcagg
     5401 tcgatggcga agcctccggg atcggcggcg acgcgcagga aaggcagctt ggcgtcggtg
     5461 cggctgcgaa ggatcagcga tgcgagccgc agcgcgaaca ccagcctcca gtcgctcgac
     5521 tcgaaacccg catcctgaac cttgcgtagc ttcccgcggt gcgcgagcac gatgcgcgcg
     5581 aggcgtgcct gctccatgcg cgagaatccc ggcatgtcgg cgttggaaag cacgtaggcc
     5641 gagtgcttgt ggtaggccgc gtgcgcgatt gacaggcccg tctccgctag ccgcgccgcc
     5701 cattcgagca tcaggcgctc cgggtcgtct tcccggtccg cgcccggcgt caggccgtca
     5761 tagatcttga gcgcgagcgc cttgacgcgc tcggcctgcg cggcatccac gtggtagcgc
     5821 cgcatgaatt gcgctaccgt agcctcgcgc atgtcgcggt gctgcacgcg gccgagcagg
     5881 tcgtacaaga cgccgtggcg cagcgcgcct tcggatacct tgatggaatc gagtccgagc
     5941 gcgtcgaagg cggcgatcat gatggcaagt ccgccgggaa gcaccggcgc gcggttggag
     6001 cggaggcccg ggatacggtc cggatccgcc ctttcctgct tgatgagcag gctgcgcagc
     6061 cgctccagcc cctcgcgcgt cagaccttcg tcggcgaagt cgttctcgcg cagaatgttc
     6121 tcgagggaac gggcggtgcc ggaggaagcg accgcttcat cccagccggt atcgcgatac
     6181 tccttcccca gcgtggcgag ctcctggctc gccgcaagct ccgcca
//
LOCUS       DASTPD010000302         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_159112, whole genome shotgun sequence.
ACCESSION   DASTPD010000302 DASTPD010000000
VERSION     DASTPD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159112"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /gene="rpmC"
                     /locus_tag="VFR57_10105"
     CDS             complement(<1..156)
                     /gene="rpmC"
                     /locus_tag="VFR57_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU0259754.1"
                     /translation="MKANELRGKSGAELQKELEGLLRAQFGLRMQLATQQLSNTSQLK
                     KVKRDIAR"
     gene            complement(159..575)
                     /gene="rplP"
                     /locus_tag="VFR57_10110"
     CDS             complement(159..575)
                     /gene="rplP"
                     /locus_tag="VFR57_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU0259755.1"
                     /translation="MLQPARTKYRKQQKGRNTGNATRGAKVSFGEFGLKATTRGRLTA
                     RQLEAGRRAMSRHIKRGGRIWIRVFPDKPVSQKPAEVRMGNGKGNPEYFVCEIQPGKI
                     IFEMDGVTDALAKEAFALASAKLPFRTIVVQRMLGQ"
     gene            complement(579..1436)
                     /gene="rpsC"
                     /locus_tag="VFR57_10115"
     CDS             complement(579..1436)
                     /gene="rpsC"
                     /locus_tag="VFR57_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU0259756.1"
                     /translation="MGQKINPVGFRLAVNRNWSSRWYANSRSFAPMLAEDIKVRDFLK
                     KKLAHASVGRVLIERPAKDARITIFSARPGVVIGKKGEEIESLKAELRRIMGVQQVHV
                     NIEEIRKPEIDAQLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNG
                     IEIARTEWYREGRVPLHTLRADIDYGLGEAKTTYGVIGIKVWVYKGDVMPKGDAAAAL
                     LAATTAPLPQPEAPVEPKRAKKPAPRAGARRGGPARPKKEGAAAAPAPKTVVKKPKKI
                     LKDEGEKKE"
     gene            complement(1436..1765)
                     /gene="rplV"
                     /locus_tag="VFR57_10120"
     CDS             complement(1436..1765)
                     /gene="rplV"
                     /locus_tag="VFR57_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU0259757.1"
                     /translation="METRASLRGARLSAQKGRLVADLIRGKPVDQAINILTYTPKKGA
                     VLVRKLLESAIANAEHNDGADIDALKVKSITVERGMFLKRFQARAKGRGNRILKHTCH
                     IYITVGD"
     gene            complement(1904..2158)
                     /gene="rpsS"
                     /locus_tag="VFR57_10125"
                     /pseudo
     CDS             complement(1904..2158)
                     /gene="rpsS"
                     /locus_tag="VFR57_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(2225..3052)
                     /gene="rplB"
                     /locus_tag="VFR57_10130"
     CDS             complement(2225..3052)
                     /gene="rplB"
                     /locus_tag="VFR57_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU0259758.1"
                     /translation="MPLVKVKPTSPGRRALVKVVTPDLHKGDPFWPLTRKQSKTVGRN
                     HHGTITMRHKGGGNKQHYRVIDFRRDKDGINARVERLEYDPNRSAHLALLLYADGERR
                     YMIAPKGLQVGAQVVSGAEAPIKTGNALPLRNVPVGTIVHCVEMLPGKGAQLARSAGT
                     GVQVLAREGSYAQLRLRSGEIRKVHIDCRATIGEAGNAEHNLESIGKAGRKRWRGVRP
                     TVRGVAMNPIDHPHGGGEGRTAAAGPPQSPWGTQSKGFKTRRNKRTQAMIVRDRRVK"
     gene            complement(3058..3336)
                     /gene="rplW"
                     /locus_tag="VFR57_10135"
     CDS             complement(3058..3336)
                     /gene="rplW"
                     /locus_tag="VFR57_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU0259759.1"
                     /translation="MNIVLAPVVSEKSTFVADKNRQYVFRVADDATKPEIKAAVELMF
                     KTKVDSVTVSNVKGKQRRFGRTIGRKRNWKKAYVRLVEGQEINFAATE"
     gene            complement(3360..3983)
                     /gene="rplD"
                     /locus_tag="VFR57_10140"
     CDS             complement(3360..3983)
                     /gene="rplD"
                     /locus_tag="VFR57_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0259760.1"
                     /translation="MELKRIAEKGTSGADTLIAKDEVFARDFNEALVHQLVVAYQANA
                     RIGSRAQKDRGAVNHSTKKPWRQKGTGRARAGMTSSPLWRGGGKIFPNSPDENFSHKV
                     NRKMYRAGMASILSQLAREDRIRVVDEFKLDAPKTKLLAQKVKAMGFDEVLVLTEEVD
                     QNLLLSSRNLENVQVMSVRNANPAALVRCAKVLLTKGAVARLEELLK"
     gene            complement(4114..4740)
                     /gene="rplC"
                     /locus_tag="VFR57_10145"
                     /pseudo
     CDS             complement(4114..4740)
                     /gene="rplC"
                     /locus_tag="VFR57_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            complement(4818..5129)
                     /gene="rpsJ"
                     /locus_tag="VFR57_10150"
     CDS             complement(4818..5129)
                     /gene="rpsJ"
                     /locus_tag="VFR57_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0259761.1"
                     /translation="MQSQRIRIRLKAFDYRLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RKQRFDLLRSPHIDKASRDQLEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(5132..>5282)
                     /gene="tuf"
                     /locus_tag="VFR57_10155"
     CDS             complement(5132..>5282)
                     /gene="tuf"
                     /locus_tag="VFR57_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0259762.1"
                     /translation="KGTEMVMPGDNVKMVVTLITPIAMEQGLRFAIREGGKTVGAGVV
                     AKIIE"
BASE COUNT          728 a         1752 c         1690 g         1112 t
ORIGIN      
        1 tcgagcgata tcccgtttca ccttcttgag ctggctggtg ttcgaaagct gctgggtcgc
       61 gagctgcatg cgcaggccga actgcgcgcg cagcaggcct tccagctcct tctgcagctc
      121 tgcaccgctc ttgcctcgga gctcgtttgc tttcatggct actgtccgag catgcgttgc
      181 acgacgatag tgcggaacgg caacttggcc gaggcgagtg cgaacgcctc cttcgccagc
      241 gcgtcggtga cgccatccat ctcgaagatg atcttgccgg gctggatctc gcacacgaag
      301 tactccgggt tgcccttgcc gttgcccatg cgcacttcgg cgggcttctg cgacaccggc
      361 ttgtcgggaa acacgcggat ccaaatccgg ccgccgcgct tgatgtggcg cgacatggcg
      421 cggcgcccgg cctcgagctg gcgcgccgtc agccggccgc gcgtcgtagc cttgaggccg
      481 aactcgccga acgagacttt ggcgccgcgc gtggcgttgc cggtgttgcg gcccttctgc
      541 tgcttgcggt atttggttct ggcgggttgc aacatcggct attccttctt ttcgccttcg
      601 tctttcagga tcttcttcgg cttcttgacc accgtcttgg gcgcgggtgc ggcggcagca
      661 ccctctttct tcggccgcgc cgggccgccg cgccttgcgc ccgcgcgcgg cgcgggcttc
      721 ttcgcgcgct tgggctcgac cggcgcttcg ggctgcggca gcggggcggt ggtggcagcg
      781 agcagcgccg cggcggcgtc gcccttgggc atcacgtcgc ccttgtagac ccagaccttg
      841 atgccgatca cgccgtaggt ggtcttcgcc tcgccgaggc cgtaatcgat gtcggcgcgc
      901 agcgtgtgca gcggcacgcg accctcgcgg taccactcgg tgcgcgcgat ctcgatgccg
      961 ttcaggcggc cggcgctcat gatcttgatg ccctgcgcgc ccaggcgcat ggcgttctgc
     1021 atggcgcgct tcatcgcgcg gcggaacatg atgcgcttct cgagctgctg cgcgatggaa
     1081 tcggcgatca gctgcgcgtc gatctcgggc ttgcggattt cctcgatgtt cacgtgcacc
     1141 tgctgtacgc ccatgatgcg gcgcagttcc gccttcagcg actcgatctc ctcgcccttc
     1201 ttaccgatca ccacgcccgg gcgcgccgag aagatggtga tgcgggcgtc cttcgccggg
     1261 cgctcgatca gcacgcggcc gaccgaagcg tgggcgagct tcttcttcag gaagtcgcgc
     1321 accttgatgt cctcggcgag cattggcgcg aacgagcggc tgttcgcgta ccagcgcgag
     1381 ctccagttgc ggttgaccgc gaggcgaaag cccacggggt tgatcttttg gcccatcagt
     1441 cacccaccgt aatgtagata tggcacgtgt gcttgagtat gcggttgccc cgccccttgg
     1501 cgcgggcctg gaagcgcttg aggaacatgc cgcgctcgac cgtgatcgac tttaccttga
     1561 gcgcatcgat gtcggcgccg tcgttgtgct ctgcgttggc gatggccgac tcgagcagct
     1621 tgcggacaag caccgcgccc tttttcggcg tataggtcag gatgttgatc gcctggtcga
     1681 ccggcttgcc gcgaatcagg tcggcgacca gccggccttt ctgcgccgag agccgcgcgc
     1741 cgcgcaggct tgctctggtt tccatttatt tcttcccgcc tgcgggcgcc gccggagcgg
     1801 ccgcaggcgc cgcaggagcg gcggccgccg gggccgcggc gccgggggcg ccgaccggac
     1861 gccggcgctc gtcgccgacc gcctccgcgg tgcgcttgcc cacggccgag tggctcttga
     1921 agatgcgcgt gagcgcgaac tcgccgagct tgtggccgac catgttctcg gtcacgtata
     1981 ccggcatgtg cgtctttccg ttgtgcacgg cgatggtcaa cccgacgaac tcgggcacga
     2041 tcgtcgagcg ccgcgaccag gtcttgatcg ggcgcttgtc attcttgctg cgggcgcttt
     2101 ccaccttctt catcaggtgg tggtccacga acggaccctt cttgattgaa cgtgccatgg
     2161 ctggttccta ggtttaggcg cgggttctag cgctcctatt ttttaggcgc gggttctagc
     2221 gctcctattt cacccgtcgg tctcgaacga tcatcgcctg cgtgcgcttg ttgcgccgcg
     2281 tcttgaatcc cttgctctgc gtgccccacg gcgattgagg cgggccggcc gctgccgtgc
     2341 ggccttcgcc gccgccgtgc ggatggtcga tcgggttcat ggcgacgccg cgcaccgtcg
     2401 ggcgaacgcc gcgccagcgc ttgcggcccg ccttgccaat cgattcgaga ttatgctcgg
     2461 cgttacccgc ttcgccgatc gtggcgcggc agtcgatgtg caccttgcgg atctcgcccg
     2521 agcgcaacct gagctgcgcg tacgacccct cgcgcgcgag cacctgcacg cccgtgccag
     2581 ccgagcgcgc aagctgcgcg cccttgcccg gcagcatctc cacgcaatgc acgatcgtgc
     2641 cgaccggaac gttgcgcagc ggcagtgcgt tgccggtctt gatcggcgcc tcggcacccg
     2701 acacgacctg ggcgccaacc tgcaggcctt tgggtgcgat catgtagcga cgctcgccat
     2761 cggcgtagag cagcagcgcg aggtgcgcgc tgcggttcgg gtcgtactcg agccgctcga
     2821 cgcgggcatt gatgccgtcc ttgtcgcgcc ggaaatcgat cacgcggtag tgctgcttgt
     2881 tgccgccgcc cttatgacgc atggtgatcg tgccgtggtg gttacggccg acggtcttcg
     2941 actgcttgcg cgtaagcggc cagaacgggt cgcccttgtg caggtcgggc gtcactacct
     3001 tgacgagagc gcgccggccg ggcgaagtag gcttgacttt tacgagaggc attcgcgtta
     3061 ctccgtggcg gcgaagttga tttcctgacc ttcgaccagg cgcacgtagg cctttttcca
     3121 gttgcgctta cgcccgatgg tgcggccgaa gcgtcgttgc ttgcccttga cgttcgacac
     3181 ggtgaccgag tcgaccttgg tcttgaacat gagctcgacc gccgccttga tctcgggctt
     3241 ggtcgcatcg tcggcgacgc ggaacacgta ctggcggttc ttgtcggcaa caaaggtgct
     3301 cttctccgat accaccggcg caagcactat gttcatcagc tgatcgatgt tgtatttctt
     3361 cacttgagca gctcctcgag ccgagccacc gcccccttgg tgagcagcac ctttgcgcag
     3421 cgcacgaggg ccgccggatt cgcgttgcgc acggacatca cctgcacgtt ctcgagatta
     3481 cgcgacgaca gcagcaggtt ctggtcgact tcctcggtga gcacgagcac ttcgtcgaag
     3541 cccatcgcct tgaccttctg ggcgaggagc ttggtcttgg gcgcgtcgag cttgaattcg
     3601 tcgaccacgc gaatgcggtc ctcgcgggcg agctgcgaaa ggatggacgc catgccggcg
     3661 cggtacatct ttcggttcac cttgtgcgag aagttctcgt ccggtgagtt cggaaagatc
     3721 ttgccgccgc cgcgccacag cgggctggac gtcatgccgg cacgcgcacg cccggtgcct
     3781 ttctgccgcc agggcttctt cgtggagtgg ttcaccgcgc cgcggtcctt ctgtgcgcgt
     3841 gagccgatgc gcgcgttcgc ctggtaggcg acgaccagct ggtggaccag cgcttcgttg
     3901 aaatcgcgcg cgaaaacttc gtccttcgcg atcaaggtgt cggcgccgct cgtgcccttc
     3961 tccgcgatcc tcttgagttc catgtcaggc cttcggcgcg ggttgcttgg cggcgggctt
     4021 cgcggccggt ttcgcctccg ccttcggctc tgccttgggc gcggccttcg ccgcctggcc
     4081 cttgagcgga gccctttgcc cctttgcggc cagacgcact accacatcgc gcccgcttga
     4141 acccggcacg gaccccttca cgagcagaag ctgccggtcg gcatcaatgc gtacgatctc
     4201 caggttggag atggtgcgcc gcacgtcgcc gaggtgccct ggcatgcgct tgccggggaa
     4261 gacgcgcgcc gggctctgat tctggccggt tgagccgggg gtcctgtgcg agatcgagtt
     4321 gccgtggctt gcgccctggg agtggaaatg atggcgcttg atcacgccgg caaatccctt
     4381 gccgatcgtc actccttgca catcgacgag ctgccccacc ttgaacagtt gctgcagcgg
     4441 aatcttcgag ccgggcttga gctcaccgag ctcatcgggc ttcgcaagct tgaattcctt
     4501 gagcacatgt ccggcttcga ccgccgcctt ggcgaggtgc ccggcgagtg gcttgttgac
     4561 gcgcgttgcg cggcgtttgc cgaatgccac ctgcacagcg gcgtagccgt ccttctccgc
     4621 ggtcttgatc tgcgtcaccc ggttgtcgga gacgtcgagc accgtgaccg gcagcgtcac
     4681 gccgtcctcg gtgaagatac gcgtcatgcc gaccttgcgg ccgaccaatc cgatcgtcat
     4741 acgtccctcg tctaattgat ccccgcttgg gcggggacgc cggctgcaat tggccggcaa
     4801 tgcacctcgt actactacta ctgaaccttg atctcgacgt ccacgcccgc cggcaagtcg
     4861 agcttcatca gcgcgtcgac cgtcttgtcc gtcgggtcga tgatatccat caaccgcaaa
     4921 tgcgtgcgga tctcgagctg atcgcgcgaa gctttgtcga tatgcggcga gcgcagcagg
     4981 tcgaagcgct gcttgcgcgt cggcaacggc accggaccgc gcaccaccgc gccagtgcgc
     5041 ttcgccgtat cgacaatctc aagcgccgac tgatcgatga gtcggtagtc gaaggctttg
     5101 agccggattc tgattctttg gctttgcatt tctactcaat gatctttgcc accacgccgg
     5161 cgccgacggt cttgccgccc tcgcggatgg cgaagcgaag cccctgctcc atcgcgatcg
     5221 gcgtgatcag cgtcaccacc atcttcacgt tgtccccggg catcaccatc tcggtgccct
     5281 tc
//
LOCUS       DASTPD010000303         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_777074, whole genome shotgun sequence.
ACCESSION   DASTPD010000303 DASTPD010000000
VERSION     DASTPD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777074"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /gene="ettA"
                     /locus_tag="VFR57_10160"
     CDS             complement(<1..1556)
                     /gene="ettA"
                     /locus_tag="VFR57_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU0259763.1"
                     /translation="MAQYVYTMRRVSKTVPPKRTILKNISLNFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDDNYEGEVVRMPGIRVGYLEQEPVLEKNKTVREAVEESLGETAAAKK
                     RLDEIYAAYSEPDADFEKLAEEQARLEAMVNTGGADEMEVAADALRLPPWEQKVDVLS
                     GGEKRRVALCRLLLSKPDLLLLDEPTNHLDAESVEWLEQFLQRFPGTVVAVTHDRYFL
                     DNAAGWILELDRGYGIPWQGNYSSWLEQKEKRLEIEASQEAARIKAMQAELEWVRQNP
                     KGRGTKSKARLARFEELSQYEHQQRNETQEIFIPVAERLGNEVIEFKDVSKGYGERLL
                     IDELSFKVPPGAIVGIIGPNGAGKSTLLRLITKREEPDSGKVEVGKTVKLAHVDQSRD
                     ELSADKTVFEDISGGQDVLKVGRFEMPARAYLARFNFRGADQQKPVGQLSGGERGRLH
                     LARMLMSGANVLLLDEPSNDLDVETLRALEEALLEFAGCVLVISHDRWFLDRIATHIL
                     AFEGDSQVVF"
     gene            1567..2076
                     /locus_tag="VFR57_10165"
     CDS             1567..2076
                     /locus_tag="VFR57_10165"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU0259764.1"
                     /translation="MIRKIALALGAGTLIAASPAALALDGAAVELGRGDDRTNLLRIS
                     FVDKWRKKQPPAGAEWRLAGYWDLSLAVWDNSNESTAEVGFTPVFRIERRSLYLEAAL
                     GFHLVQARVSADRVFSSAFQFAEHLGVGFISGKYTFGVHVQHLSNGGITEPNPGINFV
                     LVRLQYHLD"
     gene            complement(2139..3416)
                     /locus_tag="VFR57_10170"
     CDS             complement(2139..3416)
                     /locus_tag="VFR57_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0259765.1"
                     /translation="MLTKEQNELLCRVEGAAPMGGIMRRHWLPVCMLEEVAEADGKPV
                     RSRLLGEDLVIFRDSTNRIGVLSEYCLHRRASLAFGRNEECGLRCLYHGWKFDVEGNV
                     VDMPSEAPGAAQRLGKKTRAYPVREDGGFVWVWMGPKEEMRAFEPPAWAPKPDIRYAI
                     VKMHAACNWAQVLEGSIDSAHSSTLQSTNMPAAADVYGSTATESTWLRPSNDKAPRLQ
                     FEATDYGFRYAAIRKPIKNPESDQYVRTTLFIAPFTVLIPPNNQYNLAQMLVPIDDVN
                     TMFYWIAWHPDPAKGITQQAWRKFCAAEVGVDLDENFRKKRNLANDYLQDRDAMKRGD
                     WTGIVGIPAQDMAMWESMGPITDRSEDHFGSSDLAVAQFRRMMLAAATKFQQGAPAIG
                     TQKSVRYADLASFEGIVPKSTDWRTLGNTYCLA"
     gene            3432..>3932
                     /locus_tag="VFR57_10175"
     CDS             3432..>3932
                     /locus_tag="VFR57_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0259766.1"
                     /translation="MKTLRIGVAGLGRAFALMAPAFRDARVELVAAADPREEALQRFK
                     QDYAGKTFSSVDELCSDPAVDVVYVATPHEFHAAHAQLACSRKKHVLVEKPMALSLAE
                     CRAMVDSAKENGVQLLVGHSHSFDLPVLRTRQLIQNGAYGAVRMITALDFTDWVYRPR
                     RSEELGA"
BASE COUNT          638 a         1322 c         1217 g          755 t
ORIGIN      
        1 aagaacacga cctgtgagtc gccctcgaag gcgaggatgt gggtggcgat gcggtcaagg
       61 aaccagcggt cgtgcgagat gacgagcacg cagccggcga attcgagcag cgcctcctcg
      121 agcgcgcgca gcgtctcgac gtcgaggtcg ttcgacggct cgtcgagcag gagcacgttg
      181 gcgccggaca tgagcatgcg cgcgaggtgc aggcggccgc gctcgccgcc ggagagctgc
      241 ccgaccggct tctgctggtc ggcgccgcgg aagttgaagc gtgcgagata cgcgcgcgcc
      301 ggcatctcga agcgcccgac cttgaggacg tcctggccgc ccgagatatc ctcgaacacg
      361 gtcttgtccg cagacaactc atcacgcgac tggtcgacgt gggcgagctt caccgtcttg
      421 cccacctcga cctttccgct gtccggttct tcccttttgg ttattagcct gagcaacgtg
      481 gattttcctg cgccgttcgg cccgatgatc ccgacgatcg cgcccggcgg aaccttgaat
      541 gagagctcgt cgatgaggag gcgctcgccg tagcccttgg acacgtcctt gaactcgatc
      601 acctcgttcc ccaggcgctc ggcgaccggg atgaatatct cctgggtctc gttgcgctgc
      661 tggtgctcgt actgcgagag ctcctcgaag cgcgccaggc gcgccttcga cttggtgccg
      721 cggcccttgg ggttctgccg cacccactcg agctcggcct gcatcgcctt gatgcgcgct
      781 gcctcctggc tcgcttcgat ctcgaggcgc ttttccttct gctcgagcca gctcgagtaa
      841 ttcccctgcc acggaatgcc gtagccgcgg tcgagctcca ggatccaccc ggcagcattg
      901 tccaagaaat atctgtcatg cgtgaccgcg actaccgtgc ccgggaaacg ctgcaggaac
      961 tgctcgagcc actccacgct ctccgcgtcg aggtggttcg tcggttcatc gagcagcaag
     1021 aggtccggct tggacagcaa gagccggcat agcgccacac ggcgcttctc gccgccggaa
     1081 agcacgtcga ctttctgctc ccagggcgga aggcgcagcg cgtcggcggc gacttccatt
     1141 tcgtcggcgc cgccggtatt caccatggcc tcgagcctgg cctgctcttc ggcaagcttt
     1201 tcgaagtccg cgtcgggctc ggaataggcg gcgtagatct cgtccaggcg cttcttcgcc
     1261 gccgcggtct cgcccagcga ttcttcgacc gcttctctga cagtcttgtt cttttcgaga
     1321 accggttcct gttccagata gccgacgcga atccccggca tgcggacgac ctcgccttcg
     1381 tagttgtcgt cgaccccggc catgatcttg agcagcgtgg acttgcccga gccgttcagc
     1441 ccgagcacgc cgatcttcgc cccgggaaag aagttgagcg agatgttctt gaggatggtc
     1501 cgcttgggcg ggacggtttt cgacacccgc cgcatcgtgt aaacgtactg tgccatgatg
     1561 gcccgaatga tacgaaaaat cgcgctcgcg ctcggcgccg gaacgctgat cgcggcgagc
     1621 ccggccgctc tcgccctcga cggagcagcg gtggagctcg ggcgcggcga cgaccgcacg
     1681 aacctgctgc gcatctcgtt cgtagacaag tggcgcaaga aacagccgcc ggcaggcgcc
     1741 gagtggcgcc tcgcgggcta ttgggacttg tcgctcgcgg tctgggacaa ctccaatgag
     1801 agcaccgctg aggtgggctt cacgcccgtg ttccgcatcg agcgccgctc gctctacctc
     1861 gaggccgcgc taggcttcca cctcgtccag gcgcgcgtca gcgcggatcg cgtcttcagc
     1921 agcgcttttc agttcgccga gcacctcggc gtcgggttca tcagcggcaa gtacaccttc
     1981 ggggtgcacg tacagcacct gtcgaacggc ggcatcactg agccgaaccc gggcatcaac
     2041 ttcgtgctgg tgcgcctcca gtaccacctc gactagccgc acgacgtctg acgacagacc
     2101 ttccgcgtac tagcgccaga agccgcggtg ccgctctcct acgccaaaca gtaagtattt
     2161 cccagcgtgc gccagtcggt gctcttcggc acgatgccct cgaacgacgc aagatccgcg
     2221 tagcgcacgc ttttctgcgt gccgatcgcc ggtgcgcctt gctgaaattt cgtcgctgct
     2281 gcgagcatca tgcggcggaa ctgcgccact gcgaggtcgc tcgagccgaa gtggtcctcg
     2341 gaccggtcgg taatcggccc catcgactcc cacatcgcca tgtcctgcgc tgggatgccg
     2401 acgattccgg tccagtcgcc gcgcttcatg gcgtcgcggt cttgcaggta gtcgttcgcg
     2461 aggtttctct tctttctgaa attctcatcg agatcgacgc cgacctcggc cgcgcagaac
     2521 tttcgccacg cctgctgggt gatccctttc gccggatcgg ggtgccaggc gatccagtag
     2581 aacatggtgt tcacgtcgtc gatcggcacg agcatctgcg cgaggttgta ctggttgttc
     2641 ggcgggatga gcaccgtaaa gggtgcaatg aagagcgtcg tgcgcacgta ctgatcgctc
     2701 tccgggttct tgatcggctt gcggatcgcc gcgtagcgga agccgtagtc cgtcgcctcg
     2761 aactgcaggc gcggcgcctt gtcgttcgag ggccggagcc acgtcgactc ggtcgccgtc
     2821 gagccgtaaa cgtccgctgc cgccggcatg ttggtggact gcagcgtcga gctgtgcgcc
     2881 gagtcgatcg agccttccag cacctgcgcc cagttgcacg cggcgtgcat cttcacgatc
     2941 gcgtaccgga tgtccggctt cggcgcccag gcgggcggct cgaacgcgcg catctcctcc
     3001 tttgggccca tccacaccca cacaaagccg ccgtcttcgc gcaccgggta ggccctggtc
     3061 ttcttgccca ggcgctgcgc cgcgccgggc gcttccgaag gcatgtcgac gacgttgccc
     3121 tcgacgtcga acttccagcc gtggtacagg cagcgcaggc cgcattcctc gttgcgcccg
     3181 aaggcgagcg acgcccggcg gtgcaggcag tattcgctca gaacaccgat tcggttcgtt
     3241 gagtctctaa agatcaccaa gtcttcgccg aggagcctcg agcgcaccgg cttgccatcg
     3301 gcctcggcca cctcctccaa catgcacacc gggagccagt ggcggcgcat gatgccgccc
     3361 atcggcgccg cgccttcgac ccggcagagc agctcgttct gctccttcgt taacatgccc
     3421 ctatacataa catgaaaacc ctgagaatcg gcgtggctgg gctcggccgc gcgttcgcgc
     3481 tcatggcgcc ggcgttccgc gacgctcgcg tcgagctcgt cgccgccgcc gacccgcgcg
     3541 aggaggcgct tcagcgcttc aagcaagact acgcgggaaa gactttcagt tcagtagacg
     3601 aactttgctc agacccggcg gtggacgtcg tctatgtggc aacgccgcac gaattccacg
     3661 ccgcgcatgc ccagttggcc tgctcgcgta agaagcatgt ccttgtcgaa aagcccatgg
     3721 cgctttcgct cgccgagtgc cgcgccatgg tggactccgc aaaggaaaac ggggttcagc
     3781 tcctcgtcgg gcacagccat agctttgact taccggttct aaggacaagg cagttgattc
     3841 aaaacggcgc ctacggcgca gtgcggatga ttaccgcgct cgacttcacc gactgggtct
     3901 accgcccgcg ccggtccgaa gagctcggcg cg
//
LOCUS       DASTPD010000304         5347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_777983, whole genome shotgun sequence.
ACCESSION   DASTPD010000304 DASTPD010000000
VERSION     DASTPD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777983"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFR57_10180"
     CDS             <1..962
                     /locus_tag="VFR57_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259767.1"
                     /translation="AGIGLTALTLFLVLLPAFTGSYALSVATLILYFAYTGQAWNVMM
                     GFAGQLSLGHSLYVGVGAYAAGALFFHYGIGPWAGLWVAMLLCAALGLVIGFLAFRFG
                     ISGVYFALLTIAFAEFTRIGFDHIDWLGGPGGMFLKVAQRDVWDLANFRGPPLMYYYT
                     MLFLTVIAFIGCWMLLRSRAGYYWQAIRENEEAAQALGINTFRWKMAAVAISAGMTSV
                     SGVFFAFYYNNLFPEQIFHISRSIEIILGPVIGGIGTLFGPILGAVVLTLLSDGITEL
                     LAALGWEVPGIKQVFYGIVLLVVVMLLPHGIWPTVARKLKIDA"
     gene            955..1674
                     /locus_tag="VFR57_10185"
     CDS             955..1674
                     /locus_tag="VFR57_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259768.1"
                     /translation="MLEVRNISKSFRGLRAVREASFELPQGSINALIGPNGAGKTTIF
                     NMVAGVYAPDTGEIIFEGERIDGLRPDQVCAAGIGRTFQLVKPFAGLSVLDNVIVGAL
                     HRERTVGGARAYARSILKKIDLHSKAALPASSLTLPDRKRLEVARALATRPRLLLLDE
                     VMAGLRPTETDQMVQVFRDINRADGLTILLIEHVMRAVMALAQHIGVLHHGEVIARGK
                     PEEVVRNAAVLECYLGEEAEV"
     gene            1677..2381
                     /locus_tag="VFR57_10190"
     CDS             1677..2381
                     /locus_tag="VFR57_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259769.1"
                     /translation="MLSVEALDLYYGDAQALSRVSLEVGKSEIVAIIGANGAGKSSLI
                     RCIAGIEAPRSGKITFNGKIISGLESHHICNLGIGQVAEGRQVFPSLTVLENLQMGAM
                     LPRARAAAKRSLADVLALFPQLAERRHQLAGTMSGGEQQMIAIGRCLMGAPELIMFDE
                     PSLGLAPRVVQEVLHTIHVLNGRGLTILLVEQNVAVSLKISSRAYVLENGRIVMAGTG
                     DKLLHDDRVRQAYLGL"
     gene            2385..2729
                     /locus_tag="VFR57_10195"
     CDS             2385..2729
                     /locus_tag="VFR57_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259770.1"
                     /translation="MEKKLNLKKGMKVRVVGRPTNVDLAGVETTSSAKADAVIVFART
                     LAQLDAKAGPAVDAARQDRIAWVAYPKAGQLDTDLNRDVLWQHMLKKGVQAVRQVAID
                     EVWSALRFRPRK"
     gene            2726..3226
                     /locus_tag="VFR57_10200"
     CDS             2726..3226
                     /locus_tag="VFR57_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259771.1"
                     /translation="MKQVTVNDRMQRGYVYFLTEAEGENFDPEFKPQLTPPELLKIGV
                     FGGKYMSDCRAEFPAEWFERAKLCAERHDPGLNFFKVNASQPLSVWRAKGWIHPDDPR
                     GWFQWYCRYYMGRRGSDDARQIRRWRNIRRHLAQIEKHCGRRDLSCRPRQRQSLLHWA
                     YDSREV"
     gene            3290..3832
                     /locus_tag="VFR57_10205"
     CDS             3290..3832
                     /locus_tag="VFR57_10205"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0259772.1"
                     /translation="MDVRTRILEAALRLLGEGGTQQLTQPRVSKEAGVRQSHLTYYFP
                     TVTDLLQAVARHSFQSLAKELAGADARERPASLADVIAGATRDKRRVRLMLGLVQSAD
                     RDPSLKPGMRRFIRELRAGLEPLLRSAGLEARPEDIAFLHSVVIGCAVLQLARDDNEA
                     RAEARAVLEKAVSCLPKGRS"
     gene            3829..4761
                     /locus_tag="VFR57_10210"
     CDS             3829..4761
                     /locus_tag="VFR57_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0259773.1"
                     /translation="MKRLLVAVALLAGCGNEPNDAWQGYIEGEYVLLASPYAGQLQKL
                     HVRRGERIETGKPVFALEQESERAARLEAEERLKAAQARLGNLKVPKRPPEIEALRAQ
                     LNQARAARDLAALQLEQAEKLFKESFVAQTRLDEARATHERELARVREAEAQLKTALQ
                     PLGRDAEREAAESEVEAAKAALAQAAWRLEQKSVAAPVGGLVHETFFVEGEWVPAGRP
                     VAALLPPGNVKARFYVPEASLSAVSVGKDIEIRCDGCPAPIGAKVTYVSNQAEYTPPV
                     LFSRESRAKLMFLVEARLQGTNLRPGQPVDVKLK"
     gene            4758..>5347
                     /locus_tag="VFR57_10215"
     CDS             4758..>5347
                     /locus_tag="VFR57_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259774.1"
                     /translation="MSALAIDVHGLNKSFGPKHVVKDFSLEVRAGEIYGFLGPNGSGK
                     TTSIRMICGLLRPDSGHGTCLGYDIRNSAAIKREVGYMTQRFSLWEDLTIRENLDFVA
                     RMYNMNERKRAVQAQLEGLGLETRQDQLAGTLSGGWKQRLALAACMLHRPRLLLLDEP
                     TAGVDPQARRDFWEEIHRLAAGGISVLVSTHYMDEA"
BASE COUNT          922 a         1762 c         1743 g          920 t
ORIGIN      
        1 gcgcaggcat cgggctcacg gccctgaccc tgttcctcgt tctgcttccc gctttcaccg
       61 gctcctacgc gctttcagtc gccacgctga ttctgtactt cgcctacacc ggccaggcgt
      121 ggaacgtgat gatgggattc gccgggcagc tgtcgctagg tcactccctc tacgtcggcg
      181 tcggcgccta cgccgccggt gcgctcttct ttcactacgg catcggcccg tgggctggtc
      241 tctgggtggc gatgctgctc tgcgcggcgc tcggcctggt gatcggcttc ctcgccttcc
      301 gcttcggcat ctccggcgtc tacttcgcgc ttctcaccat cgccttcgcc gagttcaccc
      361 gcatcggctt cgaccacatc gactggctcg gcggacccgg cggcatgttc ctcaaggtgg
      421 cgcagcgcga cgtctgggac ctcgcgaact tccgcgggcc gcccctcatg tactactaca
      481 cgatgctgtt cttgactgtg attgcattca tcggctgctg gatgctgtta cggtcaagag
      541 ccggctatta ctggcaagcg atccgcgaga acgaggaagc cgcccaggcg ctcggtatca
      601 atacgttccg ctggaagatg gcagcggtgg cgatctcggc ggggatgacc tctgtctcgg
      661 gcgtgttctt tgcgttctac tacaacaatc tcttccccga gcagatcttc cacatcagcc
      721 gctcgatcga gatcatcctt ggcccggtga tcggcggcat cggcacgctg ttcggcccga
      781 tcctcggcgc cgttgtgctc acgctgctct ccgatggcat caccgagctg ctcgccgcgc
      841 tcggctggga ggttcccggc atcaagcagg tgttctacgg gatcgtgctg ctcgtcgtcg
      901 tgatgcttct cccccacggg atatggccaa cggtcgcgag aaaactcaag atcgatgctt
      961 gaggtccgga acatttccaa atccttccgc gggctgcgcg cggtgcgcga agcgtcgttc
     1021 gagctgccgc agggaagcat caacgcattg atcgggccga acggcgcggg caagaccacg
     1081 attttcaaca tggtcgcggg cgtgtacgca cccgacaccg gcgaaatcat cttcgagggg
     1141 gaaaggatcg acggcctgcg tcccgaccag gtatgtgcgg ccggaatcgg ccgcaccttc
     1201 cagctcgtca agcccttcgc cggtctctcc gtgctcgaca acgtgatcgt cggtgcgctt
     1261 catagagaaa ggacggtggg tggcgcgcgc gcttacgctc gttccatcct gaagaaaatt
     1321 gatcttcact cgaaagcggc tttgcctgcg tcttctctta ccctcccgga ccgcaagcgc
     1381 ctggaggtcg cgcgtgcgct ggccacgcgg ccgcggctgc tcctgctcga cgaagtgatg
     1441 gcgggcttgc gcccgaccga gaccgaccag atggtgcagg tgttccgcga catcaaccgc
     1501 gccgatggtc ttaccatcct cctgatcgag cacgtgatgc gcgcggtgat ggcgctcgcg
     1561 cagcacatcg gcgtgctgca ccacggtgag gtgatcgcac gcggcaagcc cgaagaggtc
     1621 gtgcgcaacg ctgcagtgct cgagtgctac ctcggcgagg aggcggaagt ttgagcatgc
     1681 tttccgtgga agcgctggac ctgtattacg gcgacgcgca ggcgctctcg cgcgtgagcc
     1741 tcgaggtcgg caagagcgag atcgtcgcga tcatcggcgc gaacggcgcc ggcaagtcct
     1801 cgctcatccg ctgcatcgcc gggatagaag ctccgcgctc gggaaagatc acgttcaacg
     1861 gaaagatcat ctcgggcctc gagtcccacc acatctgcaa cctgggcatc ggccaggtgg
     1921 ccgagggccg gcaggtgttc cccagtctca ccgtgctgga gaacctgcag atgggcgcga
     1981 tgctgccgcg ggcccgcgcg gcggccaagc gcagtcttgc ggacgtgctt gccctcttcc
     2041 cgcagctagc cgagcggcgc caccagctcg cgggcaccat gtccggcggc gagcagcaga
     2101 tgatcgccat cggccgctgc ctgatgggcg cacccgagct aatcatgttc gacgagccgt
     2161 cgctcggcct cgcgccgcgc gtggtccagg aggtgctgca caccatccac gtgctgaacg
     2221 ggcgtgggct gaccatcctg ctggtggagc agaacgtcgc cgtgtcgctc aagatctcga
     2281 gccgcgccta tgtgctcgaa aacggccgta tcgtcatggc gggaaccggc gacaagctct
     2341 tgcacgacga ccgcgtgcgg caggcgtatc tcgggctctg acgtgtggaa aagaagctga
     2401 acctcaagaa gggcatgaag gttcgcgtgg tcggcaggcc gaccaacgtg gatctcgcgg
     2461 gcgtcgagac gacttcgtcg gccaaggccg acgccgtgat cgtcttcgcg aggacgctcg
     2521 cgcagctcga cgccaaggca ggcccggcgg tggacgcagc caggcaagac cgcatcgcat
     2581 gggtcgctta tccgaaggcg gggcaactcg acacggatct caaccgcgac gttctttggc
     2641 agcacatgct gaagaagggc gtccaggccg tgcgccaggt ggccatcgat gaagtgtgga
     2701 gtgcgttgcg ctttcgtccc aggaaatgaa gcaagtcaca gtcaacgacc gcatgcagcg
     2761 cggctatgtt tatttcctta ccgaagcgga gggcgaaaat ttcgatcccg aattcaaacc
     2821 tcagctcacg cccccggagc tgctgaagat cggcgtgttc ggcgggaaat acatgagcga
     2881 ctgccgcgcc gagttcccgg ccgaatggtt cgagcgcgcc aagctctgcg ccgagcgcca
     2941 cgatcccggg ctgaatttct tcaaggtgaa tgcgtcgcag ccgctcagcg tgtggcgcgc
     3001 gaagggctgg attcaccccg acgacccgcg cggctggttc caatggtatt gccgctacta
     3061 catgggacgg cgcggctccg acgacgcccg ccagattcgc cgctggcgca acatccgccg
     3121 ccaccttgcg cagatcgaga agcattgcgg gcgacgcgac ctttcgtgcc gtccacgcca
     3181 gcgtcagtcg cttctgcact gggcctacga ctcgcgcgag gtgtaggcgg tcaagcactt
     3241 gacgtagcgc ttggtattct taccaaagtc ttggtaggat taccaagccg tggacgtccg
     3301 cacccgcatc ctcgaagccg cgctgcgact gctcggcgaa ggaggaacgc aacagctcac
     3361 ccagccgcgc gtaagcaaag aggccggtgt gcggcagagt cacctcacct actacttccc
     3421 cacggtcacg gacctgcttc aggctgtggc gcggcactcg ttccagtcgc ttgccaagga
     3481 actcgcgggc gccgatgcgc gcgagcgtcc tgcctcgctc gccgacgtca ttgcaggagc
     3541 gacgcgcgat aaacgccgcg taaggctgat gctcggcctg gtgcaatccg ccgaccggga
     3601 tccgagcctc aagcccggca tgcgccgctt catccgcgaa ttgcgcgctg gcttggaacc
     3661 cctcttgcgc tcggccgggc tcgaagcgag acccgaggac atcgctttct tgcatagcgt
     3721 agtgatcggc tgtgcggtgc tgcagctcgc gcgcgacgac aacgaggcgc gcgccgaagc
     3781 gcgcgcggtc ctcgagaaag ccgtcagctg cctcccgaag gggcggtcat gaagcgcctg
     3841 ctcgtcgcgg ttgccctgct tgccggctgc ggcaacgagc cgaacgacgc ctggcagggc
     3901 tacatcgaag gcgagtatgt cctgcttgcc tcgccctacg ccgggcagct gcagaaactg
     3961 cacgtgcgcc gaggcgagcg catcgagacc ggcaagccgg tgttcgcgct cgagcaggaa
     4021 tccgagcgcg ccgcgcgcct cgaggccgag gagcgcctca aagcggcgca ggcgcggctc
     4081 gggaacctga aggtgcccaa gcgcccgccc gagatcgagg cgctgcgcgc gcaactcaac
     4141 caggcgcgcg cggcgcgcga tctcgcggcg ctgcagctcg agcaggccga gaaactgttc
     4201 aaggaaagct tcgtcgcgca gacgcggctc gacgaagcgc gcgccacgca tgagcgcgag
     4261 ctcgcgcgcg tgcgcgaggc cgaagcgcag ctcaagaccg ccctgcagcc gctcgggcgc
     4321 gacgccgagc gcgaagcggc ggagagcgag gtcgaggcgg ccaaggcggc gctcgcgcag
     4381 gcggcgtggc gcctcgagca gaaaagcgtt gccgcgccgg tcgggggcct cgtgcatgag
     4441 acgtttttcg tcgaaggcga atgggttccc gcaggccggc cggtcgcggc cctgctgccg
     4501 ccgggaaacg tgaaggcgcg cttttacgta ccggaggcgt cgctgagcgc cgtttcggta
     4561 ggcaaagaca tcgagatccg ctgcgacggc tgcccggcgc cgatcggcgc caaggtgacc
     4621 tacgtatcca accaggccga atacacgccg cccgtccttt tcagcaggga atcgcgcgcc
     4681 aagctcatgt tcctcgtcga ggcacggctg cagggaacga acctgcgccc cgggcagccg
     4741 gtcgacgtga agttgaaatg agcgctctcg ccatcgacgt tcacggactg aacaagtcct
     4801 tcggcccgaa gcacgtggtg aaggacttct cgctcgaggt gcgggcgggc gagatctacg
     4861 gcttcctcgg ccccaacggc agcggcaaga ccacctcgat ccgcatgatc tgcgggctgc
     4921 tgcggcccga ctccgggcat ggcacctgcc tcggctacga catccggaac tcggcggcga
     4981 tcaagcgtga agtgggctac atgacgcaac gcttctcgct gtgggaggac ctcaccatcc
     5041 gcgagaacct cgacttcgtc gctcggatgt acaacatgaa cgaacggaaa cgcgcggtcc
     5101 aagcgcagct cgaggggctt ggccttgaaa caagacagga ccagctggcc ggcacgctct
     5161 cgggaggctg gaagcagcgg ctggcgcttg ccgcgtgcat gctgcaccgg ccgaggttgc
     5221 tgctcctcga tgaacccacc gccggcgtcg atccgcaggc ccggcgcgac ttctgggaag
     5281 aaatccatcg tctcgccgcg ggcggcatct cggtgctcgt ctccacgcac tacatggacg
     5341 aagccga
//
LOCUS       DASTPD010000305         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_778573, whole genome shotgun sequence.
ACCESSION   DASTPD010000305 DASTPD010000000
VERSION     DASTPD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778573"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1246)
                     /locus_tag="VFR57_10220"
     CDS             complement(128..1246)
                     /locus_tag="VFR57_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU0259775.1"
                     /translation="MMADTTVLSWINAEVERALTLVQESIAKFSAAPENEAVLKPCPE
                     HLHQVSGALRMVGLAGATRVCEAIENTFNGMGGARPTPAAIGVIDRAVVALKNFIGEL
                     ARGHADVPLRLFPVYRDICGLLGKTAVSEKDLFFPDLNLSAPAHASPRTVPQEERTAF
                     LQAERARFQRGLLAWLRGAPVGLRDMRQTVDALHQAATDLPEPRALWWAAGALVDSLK
                     SDAGDDPQWLASAKVVCNRIDFHIRDLGAGTAKNGDALLREVLYSLARREAATPCIRE
                     VKQLYQLETLFPRPERSVPVLELDAERLEASLFDMHSRLEALKRSWEQYVAGERNQAR
                     VFRERVADFKLRTRDLGNAHLIKLLDAIALAAAKLPDP"
     gene            complement(1243..3273)
                     /locus_tag="VFR57_10225"
     CDS             complement(1243..3273)
                     /locus_tag="VFR57_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0259776.1"
                     /translation="MKLNILGKLPAAGRRLPVLIGLLLALLAVDLGVAWYDARQATFG
                     TIYIATVGKIRMLSQRLAKAAQQASQGNLEAFKQLRESREVFAAQMSLLLNGGSVGSE
                     TLPPTSAKTRPVLEALDVQWKKNERNAALVIAEQANLANLGRAVRAINERNPALQELA
                     DEIAALSVQSGGSARQNAHAAQLMMLTQRMAKNANTMLAEAVVDPEVSFLLGKDTNSF
                     RDILRGLLQGSEALRIQRVADPELRGKLGEMEAAFKEYQAAVSGILGNQQRLVNAKRA
                     SFELFKDSEALLGAAETLNAAYEAELGARRINFIAIAVVSFLAFVVLLLIGKTYLDDT
                     RRRADENERQNRENQAAIRRLLDEISNLANGDLTVRAQVTEHMTGAIADSINYTIDEL
                     RRLVAGINSASQQVTSATQEAENVTGKLLQAASKQAAEIQGAGQSIAQMAQSMVEVSK
                     KANDSSKVATSSLHAAQKGAQAVQNAIRGMNDIRVQIQETSKRIKRLGESSQEIGEIV
                     QLISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSAEATKHIGAIVKS
                     IQRDTLEAVEAMERSTQGVVQGTKTADQAEQALREIGAVSTQLAQLIESISQATQQQA
                     TSAGRVAANMRVILGITQLTTDGTKQTATSAARLTALADGLKSSVAGFKLAA"
     gene            complement(3286..>4359)
                     /locus_tag="VFR57_10230"
     CDS             complement(3286..>4359)
                     /locus_tag="VFR57_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259777.1"
                     /translation="GAEPGVTYDRILLGQAAVFSGPAAQLGIQMRNGIRAYLDHVNAK
                     GGVHGRKLELITEDDRYEQSVAPSASKKLIEEHRVFALLGYVGTPTGVAHLPVVTQAK
                     VPLVGMFTGAEVLRVPFNRYVFHVRASYYDETEKIVEQVLSTGGQKIAVFYQDDAYGE
                     AGRKGTELALERRKMKIHSTGTVERNTVKVEQAAKTIHTSQPDAVVMVSAYTSIAAFV
                     REMQKLGSGATFYNVSFVGSKALADALGKDGVGVAISQVVPFPWGTAVPVVKEYQQLA
                     KKAGYTDYNFSALEGFLCAKVMVEGLRRAGKSPTRDGLVEALEKMNDVDLGGFYISYS
                     PMNRAGSKFVDLTIISRDGKFLR"
BASE COUNT          646 a         1472 c         1426 g          815 t
ORIGIN      
        1 gccagtttcc catgatgacg atctggagct cgaggtcgtc cgacgggttg ctgaaatggt
       61 cgatgacgtg ctcgacgagc aggaaagccg aggccatctc gatgaccatc gcctgccggt
      121 cgggggacta ggggtcgggc agcttcgccg cagccagggc gatcgcatcg agcagcttga
      181 tcaggtgcgc gttgccgaga tcccgggtcc tcagcttgaa gtcggcgacg cgctcgcgga
      241 acacgcgcgc ttggttgcgc tcgccggcga cgtactgctc ccacgagcgc ttgagcgcct
      301 cgagtcgcga atgcatgtcg aagagcgatg cctccaggcg ctcggcatcg agctcgagca
      361 ctggcacgct cctctccggg cgcgggaaca gggtctcgag ctggtaaagc tgcttgacct
      421 ccctgatgca tggggtcgcc gcctcgcgtc gggcgagcga atacagcact tcgcgcagca
      481 gcgcatcgcc gttcttcgcc gttccggccc ccaaatcgcg gatgtgaaaa tcgatgcggt
      541 tgcacacgac cttggcggaa gcgagccact gcggatcgtc tccggcatcg ctcttgagcg
      601 aatcgacgag cgcgcctgcc gcccaccaca gcgcccgcgg ctcaggcagg tcggttgcgg
      661 cctggtgcag ggcgtcgacc gtctggcgca tgtcccttaa tccgaccggc gcgccgcgca
      721 gccaggcaag cagtccgcgc tggaaacgag cacgctcggc ctgcaggaac gctgtgcgct
      781 cttcctgcgg caccgtccgc gggctcgcgt gcgcgggcgc cgagaggttg aggtcgggga
      841 agaacagatc cttctcggag accgccgtct tgccgagcag cccgcagatg tcgcgataga
      901 ccggaaacag gcgcagcggc acgtcggcat gcccccgggc gagctcgccg atgaaattct
      961 tgagcgcgac caccgcgcgg tcgatcactc cgatcgccgc cggcgtcggc cgcgccccgc
     1021 ccatgccgtt gaaagtgttc tcgatcgcct cgcatacgcg cgtcgctccc gcgaggccga
     1081 ccatgcgcaa cgccccgctc acctgatgca gatgctcggg acacggcttg agcaccgcct
     1141 cgttctcggg cgcggcggag aatttcgcga tcgattcctg aacgagcgtc agcgcccgct
     1201 caacctccgc gttgatccaa gacaggacgg tggtatccgc catcatgccg cgagcttgaa
     1261 tccagccacc gagctcttca ggccgtcagc gagcgctgtg agcctggcgg cggatgtcgc
     1321 cgtctgcttg gtaccgtcgg tggtgagctg ggtaatgccc aggatcaccc tcatgttggc
     1381 tgccaccctg ccggccgatg tcgcctgctg ctgcgtcgcc tgcgagatcg attcgatgag
     1441 ctgcgcaagc tgcgtcgaca ccgcgccgat ctcgcgcagc gcctgctccg cctggtcggc
     1501 ggtcttcgtc ccctgcacca cgccctgggt gctgcgctcc atcgcctcga ccgcttccag
     1561 ggtgtcgcgc tgaatgctct tgacgatcgc gccgatgtgc ttggtcgcct cggcggagcg
     1621 ctcggcaagt cgctgcacct cctctgccac cacggtaaag ccgcgcccgg cctcgcctgc
     1681 cgaagccgcc tggatcgcgg cattcagcgc cagcacgttc gtctgctcgg tgatgtccga
     1741 gatcagctgg acgatctcgc cgatctcctg cgagctctcg ccgagccgct tgatccgctt
     1801 cgaagtctcc tggatctgca cccggatgtc gttcatgccg cgaattgcgt tctgcaccgc
     1861 ctgcgcgccc ttctgcgcgg cgtgcagcga gctcgtggcg accttcgagg agtcgttcgc
     1921 cttcttcgag acctcgacca tcgactgcgc catctgcgcg atcgactggc cggcgccctg
     1981 gatctcggcg gcctgcttgc tcgccgcctg cagcagcttg ccggtcacgt tctcggcttc
     2041 ctgcgtggcg ctcgtcacct gctgcgacgc gctgttgatg ccggcgacca gacggcgcag
     2101 ctcgtcgatc gtgtagttga tagagtcggc gatggcgccg gtcatgtgct cggtcacctg
     2161 ggcgcgcacc gtgaggtcgc cgttggccag gttgctgatc tcgtcgagca ggcgccgaat
     2221 ggccgcctgg ttctcgcggt tctggcgctc gttttcgtcg gcccggcggc gagtgtcgtc
     2281 caggtacgtc ttgccgatga gcagcagaac gacgaacgcc aggaacgaca cgacagcgat
     2341 ggcgatgaag ttgatacgcc gtgcgccaag ctcggcttcg taggcggcgt tcagggtttc
     2401 cgcagcgccg agcagcgcct cgctgtcctt gaacagctca aagcttgcgc gcttggcgtt
     2461 caccagccgc tgctggttgc cgaggatgcc gctcaccgcc gcctggtatt ccttgaacgc
     2521 cgcttccatc tcgcccagct tgccgcgcag ctcgggatcg gcgacgcgct ggatgcgcaa
     2581 ggcttcgctg ccctggagca ggccgcgcag aatgtcgcgg aagctattgg tgtccttgcc
     2641 gagaaggaac gacacctcgg gatcgaccac cgcctcggcg agcatggtgt tcgcgttctt
     2701 cgccatgcgc tgagtgagca tcatgagctg cgcggcgtgc gcgttctggc gcgccgagcc
     2761 gccgctctgc acgctgagcg cggcgatctc gtcggcgagt tcttgcagcg cagggttacg
     2821 ctcgttgatg gcgcgcaccg cccggccgag gttggcgagg ttcgcctgct cggcgatcac
     2881 cagcgccgcg ttgcgctcgt tcttcttcca ttgcacgtcc agcgcctcga gcaccgggcg
     2941 cgttttggcc gaagtcgggg ggagcgtttc gctccccacc gagccgccgt tcaggagcag
     3001 gctcatctgc gccgcgaaga cctcgcgact ctcgcgcagc tgcttgaacg cctccaggtt
     3061 gccctgcgag gcctgctgcg ccgcctttgc gaggcgctgc gaaagcatgc ggatcttgcc
     3121 gaccgtggcg atgtagatcg tgccgaacgt cgcctggcgg gcgtcgtacc acgccacgcc
     3181 gaggtcgacg gcgagcagcg ccaggagcag accgatgagc accggcaggc gccggccggc
     3241 ggcggggagt ttgccaagga tgttgagttt catgggagcc tcgtcctagc gcaggaattt
     3301 gccgtcacgg gagatgatcg tcaggtcgac gaacttggag ccggcgcggt tcatcggcga
     3361 atagctgatg tagaagccgc cgaggtcgac gtcgttcatc ttctcgagcg cctcgaccag
     3421 gccgtcgcgc gtgggggact tgcccgcgcg ccgcaggccc tcgaccatga ccttcgcgca
     3481 gaggaagccc tccaaagcgc tgaagttgta gtcggtgtag ccggccttct tggcaagctg
     3541 ctggtattcc ttgaccaccg gcaccgccgt gccccacggg aacggcacaa cctgcgagat
     3601 tgctacgccg acgccgtcct tgccgagcgc gtcggcgagc gccttgctgc cgacgaacga
     3661 cacgttgtag aaggtggccc cgctgcccag cttctgcatc tcgcgcacga aggccgcgat
     3721 ggacgtgtag gcgctgacca tgaccactgc gtcgggctgc gaggtgtgga tcgttttcgc
     3781 cgcctgctcc acttttacgg tattgcgctc gaccgtgccc gtcgagtgga tcttcatctt
     3841 gcgccgctcg agcgcgagct cggtgccttt gcgcccagcc tcgccgtatg catcgtcctg
     3901 gtagaacacc gcaatcttct gtccgccggt tgacagcacc tgctcgacga tcttttccgt
     3961 ctcgtcgtag tagctggcgc gtacgtggaa tacgtagcgg ttaaatggca cgcgcagcac
     4021 ttccgcgccc gtgaacatgc cgaccagcgg caccttcgcc tgggtgacca caggcaggtg
     4081 cgcgaccccg gtcggcgttc cgacataccc gagaagagca aacacgcggt gttcctcgat
     4141 cagcttcttg ctcgcgctgg gcgccaccga ctgctcgtag cggtcatcct cggtgatgag
     4201 ctcgagcttg cggccgtgca ccccgccttt ggcgtttacg tgatcgaggt aggccctgat
     4261 gccgttgcgc atctggatcc caagctgggc tgcgggcccc gagaacacgg cggcctgccc
     4321 gagaaggata cggtcgtagg tgacgccggg ctcggcgcc
//
LOCUS       DASTPD010000306         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_982265, whole genome shotgun sequence.
ACCESSION   DASTPD010000306 DASTPD010000000
VERSION     DASTPD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982265"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1062)
                     /locus_tag="VFR57_10235"
     CDS             complement(100..1062)
                     /locus_tag="VFR57_10235"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0259778.1"
                     /translation="MDKPVIALAMGDPAGISPELTAKVVALKEVREAARIVVVGDRRI
                     YDDGARIAGASEMPDEFVDVKHPDPAGIRRGVAAKPGGESAQANYRHALRMVGDGAAD
                     AVCFTPFNKQAMRMAEPTYDDEILFTSNAIGFRGLATEFNVLEGLWNARVTSHIPLKD
                     VSAHLSVDKIVAALEITAAALRAAGFEPPRIGVAALNPHAGDGGNFGREEIDIIEPAV
                     RKAKAQGLPADGPYPADTVFVRAKGGQFDAVLTMYHDQGQIAMKLLGFDRGVTLLGGY
                     PFPICTPAHGTAYDIAGKGVANVGAARAAVLLAARMARRQVVMR"
     gene            complement(1071..2351)
                     /locus_tag="VFR57_10240"
     CDS             complement(1071..2351)
                     /locus_tag="VFR57_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0259779.1"
                     /translation="MDGFLVVLAFVGLFLLGFPVVLAIALPCLAYIFVKDLPLDLVAQ
                     RTLYSLDSFPLVAVPVFLFVGSVMNSAGISRYIYKFADTAVGRLPGGLAQVNIFGSLI
                     FAGMSGSALADIGGLGRIEIDAMRSKGFKPAFAAAVTSSSALVGPIFPPSIPLIIYGT
                     VTGVSVLQLLLGGIVPGILCVAMLMAMTGWLALRRGYPRTARWPTAAELWRDFKPALP
                     AILAPVILIAGMLMGYFTPTEIAAVTVLYALLISSLFYRELTWRGLLDAGYETIRSSS
                     GILLIVAVAALFGWILSVEGVPQKLTALMLQISTNPYVLLLLVNVLLLLVGMFLDSTT
                     AILVIAPIIAKPLIAAGVDPVHLGMVVVFNLMIGLLTPPMGLALFLVADIAKVTMKEV
                     LRELMPYYIPLAITLLLITYIPAITTWIPRLALP"
     gene            complement(2351..2833)
                     /locus_tag="VFR57_10245"
     CDS             complement(2351..2833)
                     /locus_tag="VFR57_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0259780.1"
                     /translation="MNRLRSTVRGAGLVLLAALIVTPLAQITMRGVFNVPLAGAEELA
                     KYFLICLTFIAAPYVTREGGQIRMEELQARLPRRPRWVLQMLIEISGVAVFGVLFVAS
                     AVTVSRNMNSVTPVLEMPFWMFFAPLVIGSLLLVIETLAMLVHTWRAGRPEPKQTVLS
                     "
     gene            complement(2830..3804)
                     /locus_tag="VFR57_10250"
     CDS             complement(2830..3804)
                     /locus_tag="VFR57_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0259781.1"
                     /translation="MKLWVAVALALGVSCANAQTKLRYAHVGVANAPQTLYADEVAKL
                     VKERTNGRVEIQVFANSQLGGVNEMVDGVRSGAISMAHHDFGSLGRIVPNTAVFNTPF
                     MYRDPEHAMRATDPAQSPALREINQELVQKGNMRIVAALFQGTRHLTSKERVLSVKDM
                     QGKKYRGVPIKLWSSMLTGMGAVATPVEVSELATALATGLVVGQENPLPNIFNLKLYE
                     VQKYVMLTGHMQSILSVFVNERVWQQIPAADRKILESTMIEVGRKTLDWDRETFGKYR
                     KELEGKGMIFVEPKDGLDVEGFRKAVLAQVNKDFPEWGKYIEQIRAVK"
BASE COUNT          710 a         1254 c         1312 g          635 t
ORIGIN      
        1 cgcgagcttc gacgaggttt atgacacctt cgatgagatc acgcaggcat tcggcgaagc
       61 ggaacgccgg gcgctgttcc acgacaacgc ggtgcggatc tatcgcatga ccacctgacg
      121 ccgggccatg cgcgcggcaa ggagcaccgc cgcgcgcgcc gcgccgacgt tagccacgcc
      181 ctttccggcg atgtcgtacg cggtgccgtg cgccggcgtg caaatcggga agggatagcc
      241 gcctagcagc gtgaccccgc ggtcgaagcc gagcagcttc atcgcgatct ggccctggtc
      301 gtggtacatg gtgagtaccg cgtcgaactg gccgcctttc gcgcgcacga acaccgtatc
      361 ggcggggtac ggcccgtcgg ccggcaggcc ctgcgctttc gccttgcgca cggcgggctc
      421 gatgatgtcg atctcttcgc ggccgaagtt gccgccgtcg cccgcgtgcg ggttgagcgc
      481 agccacgccg atgcgcggcg gctcgaagcc ggcggcgcgc agcgccgcgg cggtgatttc
      541 gagcgcggcg acgatcttgt cgaccgagag gtgcgcggag acgtccttca gcgggatgtg
      601 cgaagtgacg cgggcattcc acagtccctc gagtacgttg aactcggtgg cgagaccgcg
      661 gaagccgatg gcgttggaag tgaagaggat ctcgtcgtcg taggtcggct cggccatgcg
      721 catcgcctgc ttgttgaaag gcgtgaagca caccgcgtcg gcggcgccgt cgcccaccat
      781 gcgtagcgcg tgccggtagt tcgcctgcgc cgactcgcca ccaggcttcg ccgccacgcc
      841 gcgccggata ccggcggggt ccgggtgctt cacgtcgacg aactcatcgg gcatctcgct
      901 ggcgccggca atgcgtgcac cgtcgtcata gatgcggcgg tcgccgacca cgacgatacg
      961 cgccgcttcg cgcacttcct tcaatgccac gaccttggcg gtgagctcgg ggctgatgcc
     1021 ggcggggtcg cccatggcga gggctatcac gggtttgtcc atgctttctc ctagggtaac
     1081 gcgagccgcg ggatccacgt ggtgatggcc ggtatgtacg tgatcagcag cagcgtgatc
     1141 gccagcggaa tgtaataagg catgagctcc cgcagcactt ccttcatcgt cactttcgcg
     1201 atgtcggcga cgaggaagag tgcaaggccc atcggcggcg tgagcagccc gatcatgagg
     1261 ttgaacacca ccaccatgcc gaggtgcacc gggtccacgc cggccgcgat cagcggtttg
     1321 gcgatgatcg gcgcgatcac caggatcgcg gtggtgctgt cgagaaacat gccgacgagc
     1381 agaaggagca cgttcaccag caggagcagc acatacgggt tggtcgagat ctgcagcatc
     1441 agcgccgtca gcttctgcgg cacgccctcg accgacagga tccagccaaa gagcgccgcg
     1501 accgcgacga tcagcaggat cccggaagag gagcggatcg tctcgtagcc cgcgtcaagg
     1561 aggccgcgcc acgtaagctc gcgatagaac aggctcgaga tcagcaatgc atacagcacg
     1621 gtcaccgcgg cgatctcggt tggcgtgaag tagcccatga gcattccggc gatgaggatc
     1681 accggcgcga gaatggccgg cagcgcgggc ttaaagtcgc gccatagctc cgcagcggtt
     1741 ggccagcgcg ccgtgcgcgg gtagccccgg cgcagcgcga gccatcctgt catggccatc
     1801 agcatcgcga cgcagaggat tcccggcacg atgccgccga gcagcagctg cagcaccgag
     1861 acgcccgtca ccgtgccgta aatgatcagc ggaatcgagg gcggaaagat ggggccaacc
     1921 agggccgacg agctcgtcac cgccgcggcg aaggcgggct tgaatccctt gctgcgcatc
     1981 gcatcgatct cgatgcggcc gaggccgccg atgtcggcga gcgccgagcc cgacatgccg
     2041 gcgaagatca ggctgccgaa gatgttcacc tgcgcgaggc cgccgggcag gcggccgacc
     2101 gcggtgtcgg cgaacttgta gatgtagcgc gagatgccgg cgctattcat gacgctgccc
     2161 acgaacagga ataccggcac cgcgaccagc gggaacgagt cgagcgaata gagcgtgcgc
     2221 tgcgccacca ggtcgagcgg cagatccttg acgaagatgt atgcaaggca cggcaacgcg
     2281 atcgccagca cgaccgggaa gccgagcagg aagagaccga cgaaggcaag cacgacgagg
     2341 aagccgtcca ttaggacagc acggtttgct tcggctcggg acgcccggcg cgccaggtat
     2401 ggacaagcat ggcgagtgtc tcaatgacga ggagcagcga gccgatcacg agcggcgcga
     2461 agaacatcca gaacggcatc tcgagcaccg gcgtgacgct gttcatgttg cgcgacaccg
     2521 tcaccgcgga ggcgacgaac agaacgccga atacggcgac gccagagatc tcgatgagca
     2581 tctgcagcac ccagcgcggg cgacgcggca gccgggcctg cagctcttcc atgcggatct
     2641 gcccgccctc gcgcgtgacg tacggagccg cgatgaaagt gaggcagatc aggaagtatt
     2701 tagccagctc ttcggcgccg gcgagcggca cgttgaacac tccgcgcatc gtgatttgcg
     2761 cgagtggcgt cacgatgagc gcggccagga ggacgaggcc cgcccccctg accgtgctgc
     2821 gcagccggtt cacttaaccg cgcggatctg ctcgatgtac ttgccccact cggggaagtc
     2881 cttgttcacc tgtgcgagca ccgccttgcg gaagccctcg acgtcgaggc cgtcctttgg
     2941 ctcaacaaat atcatgccct tcccctcgag ctccttgcgg tacttgccga aggtctcgcg
     3001 gtcccagtcg agggtcttgc ggccgacttc gatcatcgtg ctttccagga tcttgcggtc
     3061 ggccgcaggg atctgctgcc acacgcgctc gttcacgaag accgagagga tcgactgcat
     3121 gtgaccggtg agcatgacgt acttctgcac ttcgtagagc ttcagattga agatgttggg
     3181 cagcgggttc tcctgcccga cgacgagtcc ggtggcgagc gcggtggcga gctcggacac
     3241 ctcgaccggg gtggcgaccg cgcccatgcc ggtgagcatg gaggaccaga gcttgatcgg
     3301 cacgccccgg tacttcttcc cctgcatgtc cttcaccgac agcacccgtt ccttcgaggt
     3361 gaggtgccgc gtgccctgaa acagcgccgc gacgatgcgc atgttgccct tctgcaccag
     3421 ttcctggttg atctcgcgca gcgcgggcga ctgcgccgga tcggtggcgc gcatcgcgtg
     3481 ctcggggtcg cggtacatga agggcgtgtt gaacaccgcc gtgttgggaa cgatgcgccc
     3541 gagcgagccg aagtcgtggt gcgccatcga aatggctccg gagcgcacgc cgtccaccat
     3601 ctcgttcacg ccgccgagct gcgaattggc gaagacctgg atctcgacgc gcccgttggt
     3661 gcgctccttc accagcttcg ccacctcgtc ggcatagagc gtctgcggcg cgttggcgac
     3721 gccgacgtgg gcgtagcgca gcttggtttg agcattggca cacgaaacgc ccagtgccag
     3781 ggcgactgca acccacagct tcatttcccc ttctcctttt cgtagagctt gtaaacggcc
     3841 gcggcatcga gctccgagta gccgcggtgc accagcattc ggtagagcga gagcgcctcg
     3901 cccatcatcg g
//
LOCUS       DASTPD010000307         6929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_371113, whole genome shotgun sequence.
ACCESSION   DASTPD010000307 DASTPD010000000
VERSION     DASTPD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371113"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="guaB"
                     /locus_tag="VFR57_10255"
     CDS             complement(<1..1142)
                     /gene="guaB"
                     /locus_tag="VFR57_10255"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU0259782.1"
                     /translation="MRVTQKALTFDDVLLLPAHSRVLPRDVSLKTRLTRKLQLNLPLV
                     SAAMDTVTEARLAIAMAQEGGIGIIHKNLPPRAQAAEVAKVKRFESGVLRDPMTIPPD
                     MPVREVIALQRERKISGFPVVNNGTVVGIVTNRDLRFETRLDQPVQAIMTPQEKLITV
                     REGTTRQEAMQLLHRHRLERVLVVGDKFQLKGLITVQDILKESEHPDASKDAHGKLRV
                     GAAVGVGEGTEERVAALVEAGADLLVVDTAHGHAQGVLDRVAWIKKNFQVEVVGGNVG
                     TAEGARALADHGADGVKVGIGPGSICTTRIVAGVGVPQITAIQNVASALKDVPLIADG
                     GVRYSGDVAKAIAAGAHCVMLGSLFAGTDESPGEIELYQGRSYKAYR"
     gene            1178..1618
                     /locus_tag="VFR57_10260"
     CDS             1178..1618
                     /locus_tag="VFR57_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEU0259783.1"
                     /translation="MKRAFLALVVAAVASNAFAQQYKWVDEMGRVRYGDLPPAGVQAT
                     PLRAPPPGAATPAPEMKKDAAKAATPKRQEDTQKEREMQAKAEQDAQIKRENCARAQD
                     VLRTMESGQRIARVDSKGERYYLDDAQIAQETAKARESVQQWCK"
     gene            complement(1619..1876)
                     /locus_tag="VFR57_10265"
     CDS             complement(1619..1876)
                     /locus_tag="VFR57_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEU0259784.1"
                     /translation="MKIEVVYALPRCARTVRLELPAGATLRDALKSCGFEVDLEKQAV
                     GVFGKRGRLDHPLAEGDRVEIYRPLALDPKEARRRRAKGRR"
     gene            complement(1866..2300)
                     /locus_tag="VFR57_10270"
     CDS             complement(1866..2300)
                     /locus_tag="VFR57_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEU0259785.1"
                     /translation="MRQIARSAIVEHSAAEMYALVEDIEAYPAFLPWCTAAVVHERRP
                     GTTKATLTVGAGRLRHSFTTQNDIRPGEAIDMHLVEGPFRRFAGRWRFAPLAPHACRI
                     EFSLSYEFSSRALGRLLAPIFHGIADSTVEAFVRRAAQVYED"
     gene            2309..2752
                     /gene="smpB"
                     /locus_tag="VFR57_10275"
     CDS             2309..2752
                     /gene="smpB"
                     /locus_tag="VFR57_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU0259786.1"
                     /translation="MSIVENRKAFHDYFIEERYEAGLALEGWEVKAIRGGRATIGEAY
                     VMVRGAEFFLIGATISPLPTVSTHFTPDPTRTRKLLLRAEEIRRLIGKVEQRGYTLVP
                     LNLHYSKGRIKLEVGLGRGKLKHDKRAAEREREWNREKQRLLRHR"
     gene            complement(2738..5116)
                     /gene="ppsA"
                     /locus_tag="VFR57_10280"
     CDS             complement(2738..5116)
                     /gene="ppsA"
                     /locus_tag="VFR57_10280"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEU0259787.1"
                     /translation="MRVAWLKDLRMADLEQVGGKNASLGEMIGGLATAGIHVPGGFAT
                     SAQAFREFLGELAPRIEERLRGLDPKDVDALAKCGAEVRSWIMRQPFPPALEEEIRSK
                     YQDLIGITSSETSFAVRSSATAEDLPEASFAGQQETFLNIRGADNVLEAIRQVYASLY
                     NDRAISYRAHHGFDHGQLALSAAVQQMVRSDLGSSGVMFTLDTESGFRDVVFITSSYG
                     LGELLVQGAVNPDEFYVHKPMLEKGRPAIVRRGLGSKLHKMVFGKSDRAGKSTRVVDV
                     GEAQRSVFSLKDTEILELARYAVAIEKHYGRPMDIEWAKDGADGRIYVLQARPETVKS
                     RTHDVEERFALKGSSRVLVSGRAIGHKIGSGTVSIIPDASQMSRVKKGDVLVTDMTDP
                     NWEPVMKLAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATRRLKDGQAVTVSCADG
                     DTGSVFEGLLPFEVSTRERGEMPRIPVKIAINVGNPQLAFEFAQLPNAGVGLARLEFI
                     INNEIGVHPKACLEHEKLPPDLKAKLLQKTKGYADPRTFYREKLVEGVATIAAAFWPK
                     PVIVRLSDFKSNEYKKLLGGERYEPDEENPMLGWRGASRYIAPDFRECFELECTAMRK
                     VREELGLTNVELMVPFVRTVGEAKEVLALLAQHGLSRGKNNLRIVMMCELPSNAILAE
                     EFLELFDGFSIGSNDLTQLTLGLDRDSGLVAGSFDERDAAVKAMLAMSIRACRKHGKY
                     VGICGQGPSDHPDLARWLMEQGIDSLSLNPDTVVSTWMALAETPAGKKSAVA"
     gene            5135..5953
                     /locus_tag="VFR57_10285"
     CDS             5135..5953
                     /locus_tag="VFR57_10285"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEU0259788.1"
                     /translation="MTQRRTVFFVSDGTGITAQMLGHSLLTQFEGVEFNQVTFPFVDS
                     TEKAEECLARISAESDSGQPIVFSTLVNNDVRAVVRKANALVIDFFESFIDPLEAGLG
                     IKSSHTIGRSHSAMDKKEYQQRIEAINFAMAHDDGASHRELGLADVILIGVSRSGKTP
                     TSLYLAMQFGVKAANYPLIPEDFHRGKLPEALRGEKSRLYGLTIASERLHEIRKERRP
                     GSRYAELDNCRFEVQEAENLMRREGIRSINSTSKSIEEIATTILRELRIQRQVY"
     gene            complement(5966..6736)
                     /locus_tag="VFR57_10290"
     CDS             complement(5966..6736)
                     /locus_tag="VFR57_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0259789.1"
                     /translation="MSVLTSRDNPKVKRWTRLAADARYRRSEKRVLIEGPHLVAAALQ
                     RGWKPVAVLATEEGAADAEIGDLIRRSGLRPVLLPKSVFRSVVDAATPPGLAAEIAIP
                     EAGRQAGAPAVFLEGVQDPSNVGAIVRSAAAFGVARVVLDRACADPWSPKSLRAGMGG
                     HFALAIEVAAKLGSELQAFKGRLVCAVPRGGLPLGEADLTGELGWIFGGEGAGLTEEA
                     VRTAALRVSVPMSRGSESLNVAAAAAICLYESRKKIGL"
     gene            complement(6733..>6929)
                     /locus_tag="VFR57_10295"
     CDS             complement(6733..>6929)
                     /locus_tag="VFR57_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEU0259790.1"
                     /translation="SAFLPYPGFVRDPYKEYSEPWFATHKALRGASFATPAGVSHARF
                     RNFYTPDRADIFAGFRTCAL"
BASE COUNT         1222 a         2319 c         2223 g         1165 t
ORIGIN      
        1 cggtaggcct tgtaggagcg gccctggtag agctcgatct cgcccggcga ttcgtcggtg
       61 ccggcgaaga gcgagccgag catcacgcag tgcgcgccgg cggcgatggc cttggcgacg
      121 tcgccggaat aacgcacgcc gccatcggcg atcaacggca cgtccttgag agccgaggcg
      181 acgttctgga tcgcggtgat ctgcggcaca cctacgccgg cgacgatgcg cgtggtgcag
      241 atcgagcccg gcccgatgcc gaccttgacg ccgtcggcgc cgtggtcggc gagcgctcgc
      301 gcgccctccg cggttccgac gttgccgccg acgacctcca cctgaaagtt cttcttgatc
      361 caggcgacgc ggtcgagcac gccctgggca tggccatgcg cagtgtcgac cacgagcagg
      421 tcggcgcccg cctccaccag cgcggcgacg cgctcctcgg tgccttcgcc tacgccgacc
      481 gcggcgccaa cgcgcagctt tccatgcgcg tccttggacg cgtcggggtg ctcgctctcc
      541 ttcagaatgt cctgcacggt gatgaggccc ttcagctgga acttgtcgcc gaccacgagc
      601 acgcgctcga gccggtgccg gtgcaggagc tgcatggcct cctgccgggt tgtgccttcg
      661 cgcaccgtga tcaatttctc ctgcggcgtc atgatcgcct gtaccggctg gtcgagccgc
      721 gtctcgaagc gcaggtcgcg gttggtgacg atgcccacca ccgtgccgtt gttcacgacc
      781 ggaaagcccg agatcttgcg ctcgcgctga agcgcgatca cctcgcgcac cggcatgtcg
      841 ggcggaatcg tcatcggatc gcgcaggacg cccgactcga agcgcttcac tttggcgacc
      901 tcggcggcct gcgcgcgcgg cggcaggttc ttgtgaatta ttccgatgcc gccttcctgc
      961 gccatcgcga tcgcgaggcg cgcctcggtc accgtgtcca ttgccgcaga aacgagcggc
     1021 aggttgagct gcagctttcg cgtcaggcgg gttttgagag atacgtcacg cggaaggacg
     1081 cgcgagtggg cgggaagtag gagaacatcg tcgaaggtca gcgccttctg tgtcacccgc
     1141 atgttttctg cgctcaaagc agcattatac tctccccatg aaaagagctt tcctcgcgct
     1201 cgtggtcgcc gcagttgcca gcaatgcatt tgcgcagcaa tacaaatggg tcgacgaaat
     1261 gggaagggtg cgttacggcg acttgccgcc cgcgggtgtg caggcaacgc cgctcagggc
     1321 gcccccgccg ggcgctgcga cacccgcgcc ggagatgaag aaggacgccg caaaggccgc
     1381 cactcccaag cggcaagaag acacacaaaa ggagcgcgag atgcaagcga aggccgagca
     1441 ggatgcgcag atcaagcgcg agaactgcgc acgggcgcag gacgtgctgc gcacgatgga
     1501 aagcggccag cgcatcgcgc gcgtcgactc gaagggcgag cgctactacc tggacgacgc
     1561 gcagatcgcg caggaaacgg ccaaagcgcg cgaaagcgtt cagcagtggt gcaagtgatc
     1621 agcgcctgcc cttagcgcgg cgccggcgcg cttccttcgg gtcgagagcg agcgggcggt
     1681 agatctcgac ccggtcgcct tcggcgagcg gatggtcgag ccgcccgcgc ttgccaaaga
     1741 cgccgacagc ctgcttttcg agatcgactt cgaagccgca cgacttgagc gcgtcgcgca
     1801 gcgtggcgcc cgcgggcagc tcgagccgca ccgtccgcgc gcacctcggg agcgcgtaga
     1861 ccacctcaat cttcatagac ctgcgcggcg cggcgcacga aagcttccac cgtcgaatcg
     1921 gcgatgccgt ggaagatcgg cgcgagtagc cgcccgagcg ctcggctcga gaactcgtag
     1981 ctcagcgaga actcgatgcg gcaggcgtgc ggtgcgagcg gcgcgaagcg ccatcgcccc
     2041 gcaaagcggc ggaacggtcc ttccaccagg tgcatgtcga tggcctcgcc gggccgaatg
     2101 tcgttctgcg tggtgaagga atggcgcagg cgcccggcgc cgaccgttag cgtagccttc
     2161 gtcgtgcccg gcctgcgctc gtgcaccacc gccgccgtgc accatggaag gaatgccgga
     2221 taggcctcga tgtcctcgac cagcgcgtac atctcggccg cggaatgctc gacaatcgcg
     2281 gaccgggcga tctgcctcat gcaagaccat gtccatcgtt gagaatcgga aagcttttca
     2341 cgattacttc atcgaagagc gctacgaggc ggggctcgcg ctcgagggct gggaggtgaa
     2401 ggcgatccgc ggcgggcgcg cgacgatcgg cgaggcctac gtcatggtgc gcggcgcgga
     2461 gtttttcctg atcggtgcca ctatcagccc gctgccgacg gtatcgacgc acttcacgcc
     2521 cgaccccacg cgcacgcgca agctgctgct gcgcgccgag gagatccggc gcctgatcgg
     2581 caaggtggag cagcgtggct acacgctggt tccgctcaac ctgcactaca gcaaagggcg
     2641 catcaagctc gaagtgggcc ttggtcgcgg caagctgaag cacgacaagc gtgcggccga
     2701 gcgcgagcgg gagtggaatc gagagaaaca gcgactgcta cgccaccgct gacttcttcc
     2761 cggcgggcgt ttcggcgagc gccatccagg tcgacaccac cgtatcgggg ttcagcgaga
     2821 ggctgtcgat cccctgctcc atcagccagc gcgcgagatc gggatggtcg gaaggcccct
     2881 ggccgcagat gcccacgtac ttgccgtgct tgcggcaggc gcggatcgac atcgccagca
     2941 tcgccttcac cgccgcgtcg cgctcgtcga agcttcccgc gaccaggccc gaatcgcgat
     3001 cgaggccgag ggtaagttgc gtaaggtcgt tcgagccgat ggagaacccg tcgaagagtt
     3061 cgaggaactc ctcggccagg attgcgttcg acggcagttc gcacatcatg acgatgcgca
     3121 ggttattttt cccgcgtgat aacccgtgtt gcgccaggag tgccaagact tccttcgctt
     3181 cccctaccgt gcggacgaaa ggcaccatca gctcgacgtt ggtcagaccc agctcctcgc
     3241 gtaccttgcg catcgccgtg cattcgagct cgaagcattc ccggaagtcg ggcgcaatgt
     3301 agcgtgaggc gccgcgccag ccgagcatcg ggttctcctc atcgggctca tagcgctcgc
     3361 ccccgaggag tttcttgtat tcgttcgact tgaagtccga caggcggaca atcaccggct
     3421 tcggccagaa cgccgcagcg atcgtcgcca cgccctcgac cagcttctcg cggtagaaag
     3481 tgcgaggatc ggcatagcct ttggtcttct gcaacagctt ggctttcagg tcgggaggaa
     3541 gcttttcgtg ctccaggcag gccttgggat gcacgccgat ctcgttgttg atgatgaatt
     3601 cgagccgcgc gagcccgacg ccggcgttgg ggagctgggc gaattcgaag gcgagctgcg
     3661 ggttgccgac gttgatcgcg atcttgaccg ggatgcgggg catctcgcca cgctcgcgcg
     3721 tggagacctc gaagggcagc agcccttcga acacgctgcc ggtgtcgccg tcggcgcacg
     3781 atacggtcac ggcttgcccg tccttcagcc gcctggtggc gtcgccgcaa ccgacgaccg
     3841 ccgggatgcc gagctcgcgc gcgatgatcg cagcgtggca cgtacgcccg ccccggttag
     3901 tgacgatcgc ggcggcgagc ttcatgaccg gctcccagtt cgggtcggtc atgtccgtaa
     3961 caagcacgtc gccttttttg acccggctca tctgcgacgc gtccgggatg atgctcaccg
     4021 tgccgctgcc gatcttgtgt ccgatcgcgc ggccgcttac gagtacgcgc gagctgccct
     4081 tgagcgcgaa gcgctcctcc acatcgtggg tgcgtgactt gaccgtctcg gggcgggcct
     4141 gcagcacgta gatgcggccg tcggctccgt ccttcgccca ttcgatatcc atcggccggc
     4201 cgtagtgctt ctcgatcgca acggcgtagc gcgcgagttc caggatttct gtgtccttaa
     4261 gagagaaaac gctgcgctgc gcctcgccaa cgtccaccac ccgggtcgat ttgccggctc
     4321 tgtccgactt cccgaacacc atcttgtgga gtttcgaacc cagaccgcgg cgcacgatcg
     4381 ccggccgccc cttctccagc atcggcttat gcacgtagaa ctcgtccggg ttcaccgccc
     4441 cctggacgag caactcgccc agcccatacg aagatgtgat gaagaccacg tcccggaagc
     4501 cggactccgt gtccagcgtg aacatgaccc cgctcgagcc caggtcgctg cgaaccatct
     4561 gctggaccgc cgcagacagt gccaattgtc cgtggtcaaa tccgtggtgc gcccggtacg
     4621 agatcgcgcg gtcgttgtag agcgaggcgt agacctgccg gattgcttcc agcacgttgt
     4681 ccgccccgcg gatgttcagg aaagtttcct gctggcccgc gaacgacgcc tcgggcaggt
     4741 cctcggcggt ggcagaagag cgcacggcga acgaggtttc gctcgaagtg attccaataa
     4801 gatcttgata ctttgagcgt atctcttcct caagcgcggg cggaaaaggc tgtcgcatga
     4861 tccacgagcg cacttcggcg ccgcatttcg ccaaagcatc gacgtccttc gggtcgagcc
     4921 cgcgcagccg ctcctcgata cgcggagcga gctcgccgag gaactcgcgg aaagcctgcg
     4981 cgctggtggc gaagccgccc gggacgtgga ttccggcggt ggcgagcccg ccgatcattt
     5041 ccccaagcga ggcgttcttg ccgccgacct gctccagatc ggccatgcgc agatctttca
     5101 gccacgctac acgcattggc gtattttagt agtcatgacg cagcgccgca ccgtgttctt
     5161 tgtttccgac ggcacgggta tcacggcgca gatgctcgga cacagcctgc tgacccagtt
     5221 cgagggcgtc gagttcaacc aggttacctt tccctttgtc gactcgaccg agaaggccga
     5281 ggaatgcctt gcgcgcatca gcgccgagtc cgacagcggc cagccgatcg tgttctccac
     5341 gctcgtcaac aacgacgtgc gcgccgtggt gcgcaaggcg aacgcgctgg tcatcgattt
     5401 cttcgagagc ttcatcgatc cgctggaagc cggcctcggg attaagtcga gccacacaat
     5461 cggccgctcg cacagcgcga tggacaaaaa ggagtaccag cagcgcatcg aggcgatcaa
     5521 tttcgccatg gcgcacgacg acggcgcctc gcaccgcgag ctgggcttgg ccgacgtgat
     5581 cctgatcggg gtgtcgcgca gcggcaagac gccgaccagc ctatacctcg ccatgcaatt
     5641 cggcgtaaag gccgcaaact acccgctgat ccccgaggat ttccaccgcg gcaagctgcc
     5701 agaggcactg cgcggcgaga agagtcgcct atacggcctc accatcgcct ccgagcgcct
     5761 gcacgaaatc cgcaaggagc gccgccccgg aagccgctat gcggagctcg acaactgccg
     5821 cttcgaggtc caagaggccg aaaacctgat gcgccgggaa ggcatccgct cgatcaactc
     5881 caccagcaag tcgatcgagg aaatcgccac cacaatcctg cgcgagctca ggatccagcg
     5941 ccaggtctac tgaccgaaat cggggtcaga gcccgatttt cttgcggctc tcgtacaggc
     6001 aaatcgccgc cgcggccgcc acgttgagcg actcgctccc gcgcgacatt gggacgctga
     6061 cgcgcaacgc ggcggtacgc actgcctcct cggtcagccc ggctccttcg ccgccgaaga
     6121 tccagcccaa ttcgccggtc aggtcagcct cgcccagggg caagccgccc ctgggaaccg
     6181 cgcaaaccag ccttcccttg aaagcctgca attcgctgcc gagcttggct gcaacctcga
     6241 tggcaagcgc gaaatgcccg cccatgccgg cgcgcaggga cttgggcgac caagggtccg
     6301 cgcaagcgcg gtcgagaacc acgcgtgcga cgccgaacgc cgccgcgctt cgcacgatcg
     6361 cgcccacgtt gctcgggtcc tgtacgcctt cgagaaaaac cgcaggcgcg cccgcctgcc
     6421 tgcccgcctc tggaatcgct atttcggcgg ccagccccgg cggcgttgcg gcgtcgacca
     6481 cgctgcggaa cacgcttttc ggcagcagga ccggccgcag gccgctccgg cggatcaagt
     6541 cgccgatttc cgcgtccgct gcgccctcct ccgtcgcaag caccgcgacg ggtttccagc
     6601 cgcgctgcaa cgcagccgcc acgagatgcg gcccctcgat cagcacgcgc ttctcgcttc
     6661 ggcgatagcg cgcatccgca gcaagcctcg tccagcgctt gacctttggg ttatcgcgcg
     6721 aggtgagtac gctcacagcg cgcaggtgcg aaagccggcg aaaatatcag cccggtcggg
     6781 cgtatagaag ttacggaagc gtgcatgcga tacgccggcg ggcgtggcga agctcgcgcc
     6841 acgcagcgcc ttgtgggtgg cgaaccaggg ctcggagtac tccttgtacg ggtcgcgcac
     6901 gaagccgggg tacggcaaga acgcgctcg
//
LOCUS       DASTPD010000308         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_982721, whole genome shotgun sequence.
ACCESSION   DASTPD010000308 DASTPD010000000
VERSION     DASTPD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982721"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFR57_10300"
     CDS             <1..1035
                     /locus_tag="VFR57_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259791.1"
                     /translation="TKARQAQSRMKMLAKMEQLAPLHVSAPFCFEFRDPERSPDPLLV
                     LESVQAGYSEKVVLRDVKLSLRSGERIGLLGVNGAGKSTLVKTIAGVLQVLSGTATFN
                     KGLEIGYFAQHQVEMLRDDESPLWHLKRISESKVREQELRDYLGSFNFPGDMASGPVE
                     NFSGGEKARLALALIVWQRPNLLLLDEPTNHLDLETREALSVALAQFEGTLILVSHDR
                     HLLRAATEKLLIVADGMVREFDGDLDDYRDWLLKKDVAPKPPRSIPAKPKPKAVNRKP
                     IETRIKRLEELMARLGARKVEIEKKLGDPALYQDGAELKALLEDQAYVAREIEQVEAE
                     WLEKQAELEP"
     gene            1032..2234
                     /locus_tag="VFR57_10305"
     CDS             1032..2234
                     /locus_tag="VFR57_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0259792.1"
                     /translation="MKHPDLKARIDATAARVSPGLVELRKKLHQHPELAFEEHETAKV
                     VKGFLEKLGLSVKAGVGKTGVVAVLQGGRPGRTIGVRADMDALPIHEQTGLPFASRLP
                     GRMHACGHDVHTVIALGVAAALAEMKDRLQGRIKFIFQPAEETLSGARAMIADGVLQE
                     PDLDLILGYHNWPAVQAGRVGYNTGAVTSAADAFDITLKGRDGHGAHPHLAVDALAAG
                     AHFVSQLQTVVSREVKPVSPAVVTVGEFHAGRTRNVIAGTAELKGIVRTLEPGLSERI
                     EAAVRRMLEGLKTGMRVDYTLAWNRMAPALRNDPDVLKKVLSSAREALGAENVLELPE
                     PSMGSEDFAWFAQRVPAAHLRIGAKIDGLDTAIHRSDYDCNELAIPVGIRVLARAALD
                     LGTSGTKR"
BASE COUNT          450 a          779 c          826 g          408 t
ORIGIN      
        1 accaaggcac gccaggcgca gagccgcatg aagatgctcg ccaagatgga gcagctcgcg
       61 ccgctgcacg tctcggcgcc gttctgcttc gagttccgcg acccggagcg ctcgcccgat
      121 cctttgttgg ttttggaatc cgttcaggcg ggttactcgg agaaagtagt gctgagagac
      181 gtcaagcttt cactccgatc gggagagcgt atcggcctcc tcggcgtcaa cggcgccggc
      241 aagtccacgc tggtgaagac cattgcgggg gtgctgcaag tgctatctgg aactgccacc
      301 ttcaacaaag gcctcgagat cggctacttt gcccagcacc aggtcgagat gctgcgcgac
      361 gacgagtcgc cgctctggca tttgaagaga atttcggaaa gcaaagtccg ggaacaggag
      421 ctgcgcgatt acctcggcag cttcaacttc cccggcgaca tggcgagcgg cccggtggaa
      481 aacttctccg ggggcgagaa ggcgcggctc gcgctcgcgc tgatcgtctg gcagcggccg
      541 aacctgctgt tgctcgacga gccgaccaac cacctcgatc tcgaaacacg cgaggcgctg
      601 agcgtggcgc tcgcgcagtt cgaaggcacg ctgatcctcg tctcgcacga ccgccacctg
      661 ctccgcgccg ccaccgagaa gctgcttatc gtcgccgatg gcatggtgcg cgagttcgac
      721 ggcgacctcg acgactaccg cgactggctg ctaaaaaagg acgttgctcc taagcccccc
      781 aggagcattc ctgccaaacc caaacccaaa gccgtcaacc gcaagccgat cgagacgcgc
      841 atcaagcgcc tggaggagct gatggcccga ctaggcgctc gcaaggttga gatagagaaa
      901 aagctcggcg atccggcgct ctaccaggat ggcgccgagc tgaaggcgct gctcgaggac
      961 caggcctacg tggcgaggga gatcgagcag gtggaggcgg agtggctgga gaagcaggca
     1021 gaattggagc catgaagcac ccggacctca aggcgcgcat cgatgcgacc gctgcgcgcg
     1081 tttcgcccgg gctggtggag ctgcgcaaga agctgcatca gcaccccgag ctcgccttcg
     1141 aagagcacga aaccgcgaaa gtggtcaagg ggtttctcga gaagctgggt ttgtcggtga
     1201 aggccggtgt cggcaagacc ggggtcgttg cagtgctgca aggcggccgg cccggccgca
     1261 cgatcggcgt ccgggccgac atggacgcgc tgccgatcca cgagcagacc ggcttgccgt
     1321 tcgcatccag gctgccgggc aggatgcacg cctgcggtca cgacgtgcat accgtgatcg
     1381 ctctgggcgt ggcggccgcg ctcgcggaaa tgaaggaccg gctgcaggga cggatcaagt
     1441 tcatcttcca gccggcggag gagacgcttt ccggcgcgcg cgcgatgatc gccgacggcg
     1501 tgctccagga gccggatctc gacctcatcc tcggctatca caactggccg gcggtgcagg
     1561 ccggcagggt cggctacaac accggcgccg tcacctccgc ggccgacgct ttcgatatca
     1621 cgctcaaggg tcgcgacggc cacggcgcgc atccgcacct cgcggtcgac gcgctcgccg
     1681 cgggcgcgca tttcgtgtcg cagctccaaa cggtggtgag ccgcgaggtc aagccggttt
     1741 cgccggcagt tgtcaccgta ggcgagttcc acgccggccg gacgcgtaac gtcattgccg
     1801 gcacggctga gctgaaaggc atcgtgcgga ctttggagcc gggtctgtcg gagagaatcg
     1861 aggcggcggt gcggcgcatg ctggaagggc tcaagactgg catgcgagtg gactacacgc
     1921 tcgcctggaa ccgcatggcc cccgcgctgc gcaacgatcc cgatgtactc aagaaagtgc
     1981 tttccagcgc ccgtgaggcg ctcggtgcgg aaaacgtgct ggagctgccc gagccgtcga
     2041 tgggcagcga agacttcgcc tggttcgccc agcgtgtgcc ggcggctcac ttgcgcatcg
     2101 gcgcgaagat cgacggcctc gacactgcga tccaccgctc cgactacgac tgcaacgagc
     2161 ttgcgatccc ggtcgggatc cgggtgctgg cccgggcggc gcttgacttg gggacttcgg
     2221 gaacgaagcg ctaaaggtcg cccctgcgct tttcccgcgg cggctcctgc ttttcggaat
     2281 tgcgcttgcg gtccacgcgc cgctcacgca atgatcatgc cggatgaaat ggttcgatct
     2341 ctcgaggacg gtcgtgctgc cgctgcattg cgacggctac ggccacatga acgtgcgcaa
     2401 ctacgccggg ttcttcgacg atgccggctg gcacatgctt gccatggcgg ggatttcgct
     2461 gag
//
LOCUS       DASTPD010000309         6412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_575641, whole genome shotgun sequence.
ACCESSION   DASTPD010000309 DASTPD010000000
VERSION     DASTPD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575641"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..659
                     /locus_tag="VFR57_10310"
     CDS             297..659
                     /locus_tag="VFR57_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259793.1"
                     /translation="MRTAVVALFVALAGAFATEPARGHHRSHVTVGVGFGFGYPYWGP
                     GWWGPWGPWYYPGPYYYPAPTVVVPSQPVTYIEKGSGAPAPESWWYYCDASKGYYPYV
                     KECPSGWQRVPPAPPPSR"
     gene            656..1189
                     /locus_tag="VFR57_10315"
     CDS             656..1189
                     /locus_tag="VFR57_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEU0259794.1"
                     /translation="MKRLGFGLAVFLLSSCAATMPSGPGVLALPGTGKSFDQFRADEQ
                     ECRGQAYVQIGGKNAEQAASDAASRSALIGTAIGAAMGGLFGGAEGAAVGAAFGLAGG
                     AIVGSDASYAAGSSLQRRYDHAFMQCMYEKGHKVPVPAGRYPTPGNGVQSGTRTPPPP
                     PPPGQPPPEAPPDYRPQ"
     gene            1199..2716
                     /locus_tag="VFR57_10320"
     CDS             1199..2716
                     /locus_tag="VFR57_10320"
                     /inference="COORDINATES: protein motif:HMM:NF008868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0259795.1"
                     /translation="MLELRSYVGGRWIAGTGAAQTLLNPATEEPLARASSEGVDFKSA
                     LEHARSVGGPALRAMSFVERGAALKAVADAIQSEREPLLDLAIANGGNTRSDAKFDVD
                     GAIATLLAYAEISNSLGAGKFLPDAEGIQLGRSARFHGQHVLVPRRGVAVHINAFNFP
                     AWGFAEKAAVALLAGMPVLSKPATSSALVAHRIMELIVEKKLLPEGALSFLVGGAGDL
                     VSHLASQDVLAFTGSGETGAKLRSSQSIIEQAVRVNVEADSLNAAVLGPDVEPRSDMY
                     ELFLKDVVRDMTQKAGQKCTAIRRIVVHENRVSSVRDDLAELLATIRVGDPKSEDTRM
                     GPLASAAQLADVREGVQRLGGRHAFGSIGALGPKGFFISPVLVEDPGETVHEREVFGP
                     VASLVCFSENPAEIVARGNGGLVCSVYSEDQDFVEAAVGELAPYHGRIYLGHPKIELS
                     PGPGTVLPQLVHGGPGRAGGGEELGGPRGMAFYMQRVALEGSRPVIAKILGAKSA"
     gene            2738..3496
                     /locus_tag="VFR57_10325"
     CDS             2738..3496
                     /locus_tag="VFR57_10325"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0259796.1"
                     /translation="MDRLKGKVAIVTGAGSGIGKALARRLAADGASVVIADVARFGET
                     AAEITKETGARTLGLQIDVSAEKDAERMAAEAVRAFRRIDVLVNNAAVFSSLKLKPFE
                     EIGVDEWRKVMDVNIMGVALCCRACVPHMRKAGGGRIINLASGAPIKGVPLFLHYISS
                     KGAVIAMTRGLARELGKDGITVNSLAPGFTLSENVLAKEDHMKAGAFTRGTRAIPRDE
                     TPEDLVGAVSFLASADAAFITGQTLVVDGGSAMI"
     gene            complement(3518..4663)
                     /locus_tag="VFR57_10330"
     CDS             complement(3518..4663)
                     /locus_tag="VFR57_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0259797.1"
                     /translation="MKLLEQEYPRFSDGEYARRHCALELVMQSHDVDHLLIVTDHRAG
                     NAPQWVTGWPGTVEAYVIFKPGERMTMHVEWFNHLPLAIRVAHDVNVRWGEHRGVEKT
                     IEELKRRGAKRVGLIGPLVVPKYKLMESEFAVVLLDAEYVRLRLVKSEEEIEWLRLGA
                     ALSDAGFRELLDHTRPGLTERELANLVERAYVGRGGTTFIHYIGATSMAAPHICVPPQ
                     FASSRKVTPGDIVFCELSASWWDYAGQVLRTFTVGTEATALYRDLHATAEAAFDAITG
                     KIHHGTTMEEIVEASGVIEDRGFTIYDDLMHGFGGGYFPPILGSRSRPAGPLPAMKLE
                     ENMTIVVQPNVVTPDHKAGVQVGELVRVTRTGFERLHSAPRGFFRAG"
     gene            complement(4660..5613)
                     /locus_tag="VFR57_10335"
     CDS             complement(4660..5613)
                     /locus_tag="VFR57_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259798.1"
                     /translation="MKRLLAGLLVLIVTQVQAQAWPAKPVKIIVPFAPGGTADTLGRL
                     AAQKLGEQLKASFVVENRAGAGGALGSDLVAKAAPDGYTLVISGIASHVIAPMLPQGT
                     PYDPVRDFTHIALFGGSPAVLAVHAQLPAKDLPALIALAKSGHRLSYGSPGNGTQGHL
                     VAELFKQRAGINMQHIPYKGASGAMTDLIAGHIPVVSTTLTTAAPQIRAGRARGLAIS
                     AASRLAEFPDIPTYGELGYPDLVATVWFSLSGPANVPADVVERLNAEVNRAFELAEVR
                     ERLKPEGIVPSRMSAKEFGAFVAEELRRWGPVVRASGARND"
     gene            5637..>6412
                     /locus_tag="VFR57_10340"
     CDS             5637..>6412
                     /locus_tag="VFR57_10340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0259799.1"
                     /translation="MNEVIDVHTHIVPEKFPSYRGKGRDVPWPSMADAHACHKHVMIS
                     GKLYRTVSDGSWSVPRRIEQMSEMRVTRQVLSPMPELLSYWLPLDDAKVLIRYLNEQI
                     AELLQAAPERFVGLGCVPLQDTDSAIGELQFLKSDLNFPGVEIASHVNGVSIGDARFE
                     PFFAAAERLGAAIFVHALRPAGQERIVGAFPEQAVCFPGDVALACASMITGGIASRHP
                     KLRIAFSHGGGTMSILMPRLVHAWNNFPKAKESLQESPAT"
BASE COUNT         1129 a         2108 c         2094 g         1081 t
ORIGIN      
        1 cttcgcgaag gcgtggcgga cgagcgtgtt aaggacaggt catctacatg acacccgcac
       61 ttttccgcgc aactgaggca tcagggccgc gcgatcctcg gaagttgctc gggcacgacg
      121 aggcgggtaa ctgcgaaaat gtatctgcgc ggccgcgacg tctaggttat tcatgggcgg
      181 agtattaggc gccaataggc gccgcctgaa aatgtctcaa gaagcataaa cttgcctacg
      241 tgccaagtaa caatcggtta ctactttcgc tgcatagcgg gtgtaggctg aagcgcatgc
      301 gtaccgcggt tgttgcgctc ttcgttgcac tagccggggc cttcgccacc gaacccgcgc
      361 gcggccatca ccgcagccac gtaaccgtcg gcgtcgggtt cgggttcggc tacccctatt
      421 ggggaccggg ctggtggggg ccttggggcc cgtggtatta ccctggcccc tactactatc
      481 cggcaccgac ggtcgtagtt ccttcacaac cggtcaccta tattgaaaaa ggcagcggcg
      541 cgcccgctcc cgagtcgtgg tggtactact gcgacgcgtc gaaaggctat tacccgtacg
      601 tgaaggagtg cccgagcggc tggcagcgcg tgccccctgc gcctccgccc tcgagatgaa
      661 aaggctcggt ttcggcctcg ccgtattcct gctgtcgtcg tgcgctgcga ccatgccgag
      721 cggccccggc gtgctcgcgc tgccgggcac cggcaagagc ttcgaccagt ttcgtgccga
      781 cgaacaggag tgcaggggcc aggcgtacgt gcaaatcggc ggcaagaatg ccgagcaggc
      841 cgccagtgac gccgcaagca gaagcgcctt gatcggcacc gcaatcggcg ccgcgatggg
      901 cggactcttc ggcggtgcgg aaggcgcggc ggtcggcgcg gcgttcgggc tcgccggcgg
      961 cgcgatcgtc ggaagcgatg cttcctatgc cgcgggcagc tcgctgcagc ggcgctacga
     1021 ccacgccttc atgcagtgca tgtacgagaa aggccacaag gttccggtgc cggcagggcg
     1081 gtatccaacc cccggcaacg gcgtgcaatc cggaacgcgc acgccgccgc caccgccgcc
     1141 gcccggacag ccgccgcccg aagcgccgcc cgactaccgc ccgcagtaat attccgggat
     1201 gctggagttg cgcagctacg tcggcgggcg ctggatagct ggaaccggcg cagcgcagac
     1261 cctgctcaat cctgcgaccg aggagccgct cgcccgggcg agcagcgagg gcgtcgattt
     1321 caaatccgcg ctggaacacg cccgctcggt cggggggccg gcgctgcgtg cgatgagctt
     1381 cgtcgagcgc ggcgcggcgc tgaaagccgt cgcggacgcc attcaaagcg agcgcgaacc
     1441 actccttgac cttgccatcg caaacggcgg caacacccgc tcggacgcca agttcgacgt
     1501 cgacggcgcg atcgccacac tgcttgctta tgcagagatt tccaactcac tgggcgcggg
     1561 aaagttccta ccggacgcag aaggcatcca gctcggccgc tcggcgcggt ttcacggcca
     1621 gcatgtgctg gtgccacgcc gcggcgtggc ggtgcacatc aacgcattca attttccggc
     1681 ctggggattt gcggaaaagg cggccgtagc gctccttgcc ggcatgccgg tgctgagcaa
     1741 gccggcgacg agctccgcgt tggtcgcgca tcgcatcatg gagctcatcg tcgagaaaaa
     1801 gctgctgcct gaaggcgctc tttcgttcct cgtcggcggc gctggcgacc tcgtgtccca
     1861 tctcgcctcg caggacgtac ttgcctttac cggttcgggc gaaaccggag cgaagttgcg
     1921 gtcttcacaa agcattattg agcaggccgt tcgggtaaac gtggaagcag acagcctgaa
     1981 cgcggcagtc ctcggcccgg acgtcgagcc gcgcagcgat atgtacgagc tcttcctcaa
     2041 agacgtggtg cgcgacatga cgcaaaaagc cgggcagaaa tgcactgcga tccgccggat
     2101 tgtggttcat gaaaaccggg tttcttcggt aagagacgat ctcgcagaac tgctcgccac
     2161 gattcgcgta ggcgacccga agtcggagga cacgcgcatg ggcccgctcg cgagcgccgc
     2221 gcagctcgcc gacgtgcgcg agggcgtgca gcgcctgggc ggcaggcatg ccttcggcag
     2281 catcggggca ctcggcccca agggtttctt catctcgcca gtgctggtcg aggatccggg
     2341 cgaaacggtg cacgagcgcg aggtgttcgg ccccgtcgct tctcttgttt gtttctctga
     2401 aaatccggct gaaatcgtgg ctcgcggtaa cggcgggctc gtctgctcgg tgtactccga
     2461 ggaccaggac ttcgtcgagg cagcggtagg cgagctcgcg ccctaccacg ggcgcatcta
     2521 tctcggccac ccgaagatcg agctttcgcc cgggccgggc accgtgctgc cgcagctcgt
     2581 gcacggcggg ccggggcgcg ccggcggcgg cgaggagctc ggcggcccgc gcggtatggc
     2641 gttctacatg cagcgtgtcg cgctcgaggg ctcgcgccct gttatcgcaa agattctagg
     2701 agcgaagtcc gcataaccgg ggtcagaccc cgcccaaatg gatagactca agggaaaggt
     2761 tgccatcgtg accggcgcgg gcagcgggat cggcaaagcg ctcgcccggc gcctcgccgc
     2821 tgacggcgct tcggtcgtga tcgccgatgt cgcgcgcttt ggcgagacgg ctgccgagat
     2881 caccaaagag accggcgccc gcacgctcgg actgcaaatc gacgtaagtg ctgagaaaga
     2941 tgccgagcgc atggcggctg aagcggtgag ggcgttcagg cgcatcgacg tcctggtgaa
     3001 caacgccgcg gtcttcagca gcctgaagct caagccattc gaggaaatcg gcgtcgacga
     3061 atggcgcaag gtgatggacg tcaacatcat gggagtggcg ctctgctgcc gcgcgtgcgt
     3121 gccgcacatg cgcaaggcgg gcggcggccg catcatcaac ctcgcctccg gcgcgccaat
     3181 caagggcgtg ccgctcttcc tgcactacat ctcgagcaag ggcgcggtga tcgccatgac
     3241 gcgcgggctt gcgcgcgagc tcggaaagga cggcataacc gtgaactcgc tggcgcctgg
     3301 attcacgtta tcggagaacg ttcttgccaa ggaagatcac atgaaggctg gcgcgttcac
     3361 gcgcggcaca cgcgccattc cgcgtgacga aacgcccgag gacctcgtcg gcgccgtgag
     3421 cttcctcgcc agcgccgacg ccgccttcat caccggccag accctcgtcg tcgacggcgg
     3481 ctcggcgatg atttagtgag gcaccggaag cgcctcgtca gcccgcccgg aagaacccgc
     3541 gcggcgcaga gtgcagccgc tcgaagcccg tccgggtcac gcggacgagc tcgccaacct
     3601 ggacacctgc cttgtggtcg ggggtcacga cattcggctg gacgacgatc gtcatgttct
     3661 cctccagctt catcgcgggt agcggccccg cggggcggct gcggctcccc aggatcggcg
     3721 ggaaatagcc gccgccgaag ccgtgcatca agtcgtcgta gatggtgaag ccccgatcct
     3781 cgatgacgcc ggaggcctcg acgatctcct ccatcgtcgt cccgtgatga atcttgcccg
     3841 taattgcatc gaaagccgct tctgccgttg cgtgcaaatc gcggtatagc gccgtcgctt
     3901 cagtgcctac ggtaaaggtg cgcagtacct gccccgcgta gtcccaccag ctcgctgaaa
     3961 gctcgcagaa cacaatgtcg cctggcgtaa ctttccgcga agaagcgaat tgcggcggca
     4021 cgcagatatg cggcgccgcc atcgaggtcg cgccgatgta gtggataaag gtcgtgccgc
     4081 cccgcccgac ataggcccgc tccacaagat tggcgagctc gcgttccgtc aatcccggtc
     4141 ttgtgtggtc aagaagttcc ctgaacccgg cgtcgctcag agctgcgccg aggcgcagcc
     4201 actcgatctc ctcctcggac ttcaccagac gcaagcgcac gtattcagca tcgagcagca
     4261 ccacggcaaa ttcgctttcc atgagcttgt acttcgggac tacgagcggc ccgatgagcc
     4321 cgactcgttt cgcgccgcgc cgcttgagct cctcgatggt cttctccacg ccgcgatgct
     4381 cgccccagcg cacgttgacg tcgtgcgcaa cgcggatcgc gagcggaaga tgattgaacc
     4441 actcgacatg cattgtcatc cgctcgccgg gcttgaaaat cacgtacgcc tcgactgtgc
     4501 ccggccagcc ggtgacccac tgcggcgcgt tgccggcgcg atgatcggtg acgatcagga
     4561 ggtgatcgac atcgtgactt tgcatcacga gctccagggc acaatggcgg cgcgcatact
     4621 cgccgtcgga gaagcgcggg tattcctgct cgaggagctt caatcgttcc tggcgccgga
     4681 ggcgcgcact accgggcccc agcgtcgaag ctcctcggcg acgaaagcgc cgaattcctt
     4741 ggcgctcatg cgactcggca cgatgccctc gggcttgagc cgctcgcgca cctcggcaag
     4801 ctcaaacgcc cgattcacct cagcgttcag ccgctcgacg acatccgcag gcacgttcgc
     4861 cgggccggag agcgagaacc acaccgtcgc aacgaggtcc ggataaccga gctcgccata
     4921 cgtcgggata tcgggaaact ccgccaggcg cgaagcggcg ctgatggcga ggccgcgcgc
     4981 ccgcccggcg cggatctgtg gcgccgcggt cgtcagcgtg gtcgacacga ccgggatgtg
     5041 tccggcgatc agatctgtca tggcgccgga cgcgcccttg taggggatgt gctgcatgtt
     5101 gatgcccgcg cgttgcttga acagctcggc gaccaggtgg ccttgcgtac cgtttcccgg
     5161 tgacccatac gacaagcgat gtccggattt ggcaagagcg atcagcgcgg gcaggtcctt
     5221 cgcgggcaac tgcgcgtgca ccgcgagcac ggctggagat ccgccaaaga gcgcgatgtg
     5281 cgtgaaatcg cgcaccgggt cgtacggcgt gccctgcggc agcatcggcg cgatgacgtg
     5341 cgaggcgatg cctgagatca ccagcgtgta gccgtcgggc gccgccttgg cgacgaggtc
     5401 cgagccgagc gcgccaccgg cgcccgcgcg attctcgacc acgaagctcg ccttcagctg
     5461 ctcgccgagc ttctgcgccg cgaggcggcc gagcgtgtcg gcggtgccgc ccggcgcgaa
     5521 cggaacaatg atcttcactg gtttggcggg ccaggcctgt gcttggactt gggtgacgat
     5581 aagaacaagc aaaccggcga gcaggcgctt catggctcga ttctataatc ggccgcatga
     5641 acgaagtcat cgacgtccat actcacatcg tccccgagaa atttccctcg taccgcggca
     5701 aagggagaga cgtgccctgg ccgtccatgg ccgacgcaca cgcgtgccac aagcacgtga
     5761 tgatctccgg caagctctac cgcactgtga gcgacggctc ctggagcgtg ccgcgccgca
     5821 tcgagcagat gtcggaaatg cgcgtgaccc ggcaggtgct ctcgccgatg ccggagctcc
     5881 tgtcgtactg gctgccgctc gacgacgcta aagtgctgat ccgctacctc aacgagcaga
     5941 tcgccgagct gctccaggcc gcgcccgagc gcttcgtcgg gctgggctgc gtgcccctcc
     6001 aggatacgga cagcgcgatc ggggaactgc aattcctgaa gagcgatttg aatttccccg
     6061 gtgtagagat tgcgagccac gtcaacgggg tctcgatcgg agacgcacgc ttcgagcctt
     6121 tcttcgccgc ggccgagcgg ctcggcgccg cgatcttcgt ccacgcgctg cgccccgcag
     6181 gccaggagcg catcgtcggc gcattccccg agcaggcggt gtgctttccc ggcgacgtcg
     6241 ccctcgcctg cgcctcgatg atcaccggcg gcatcgcctc gcgccatccg aagctgcgca
     6301 tcgccttcag ccacggcggc ggcacgatgt cgatcctgat gccgcgcctg gttcacgcct
     6361 ggaacaattt cccgaaggcg aaggagtcgc tgcaggaatc ccccgcgacg at
//
LOCUS       DASTPD010000310         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_372696, whole genome shotgun sequence.
ACCESSION   DASTPD010000310 DASTPD010000000
VERSION     DASTPD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372696"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFR57_10345"
     CDS             complement(<1..139)
                     /locus_tag="VFR57_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEU0259800.1"
                     /translation="MSTESQVQFRTWMCLICGFVYDEAAGLPDEGIAPGTRWEDVPMN
                     WT"
     gene            163..912
                     /locus_tag="VFR57_10350"
     CDS             163..912
                     /locus_tag="VFR57_10350"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEU0259801.1"
                     /translation="MLPIVLTFAASDPTGGAGLQADVLTLAALGCHPLSVLTGLTVQD
                     TRGVDHLEPIAEELVTRQANCVLAESKVAAFKVGVLASADNARAVGQVAAAHAHVPLV
                     LDPVLASGRGDELAGNAVQAALLEWLVPRATVVTPNTVEAQRLGGVKHLLERGCRYVL
                     VTGTHEPSAEVTNKLYGPGGLVREDRWQRLPELFHGSGCTLASAIAAQLAKGRAVSEA
                     VREAQEFTWRALAAGFRSGAGQWLPNRFFPR"
     gene            912..1508
                     /gene="thiE"
                     /locus_tag="VFR57_10355"
     CDS             912..1508
                     /gene="thiE"
                     /locus_tag="VFR57_10355"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0259802.1"
                     /translation="MRGLYPITPNTADTDALLRKVEEALRAGVAMLQYRNKSISKDKR
                     LLQAKELAPLARGYGVPLIVNDDVEIALAVGANGAHLGEDDGDLAAARVKLVGKILGA
                     SCYDSLESARTAVRSGADYVAFGAVYPSPTKPDAKRAPLSLFGHDLGVPLCAIGGITL
                     ENAPAVIAAGASLLAVISDIFDAPDIGARASQYRKLFS"
     gene            1505..2785
                     /gene="hemL"
                     /locus_tag="VFR57_10360"
     CDS             1505..2785
                     /gene="hemL"
                     /locus_tag="VFR57_10360"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0259803.1"
                     /translation="MTRSKDLFERAQARIPGGVNSPVRAFQAVGGTPVFFERASGPYL
                     WDVDGKRYIDYVGSWGPMLAGHGHPTVVEAVQEAASRALSFGAPTEAEIDLAALLCRE
                     LPSLELVRLVSSGTEATMTALRLARGFTGRSLIVKFEGCYHGHADALLVKAGSGALTF
                     GNPSSAGVPAQVAAQTVVLDYNDVYQVQNLFAEKGQEIAAVIVEPVAGNMNLVLPAPG
                     FLEALREACTRHGAVLIFDEVMTGFRVAAGGAQQRFRIRPDLTTLGKVIGGGLPLAAV
                     GGRRDIMERIAPLGPVYQAGTLSGNPIAVAAGLATVRLVTQEGFHEKVQRTTAELVHG
                     LSVEAKKAGIVFCAQSIGSMFGIYFRAVPPTSFAEVMQCDRARFNRFFHEMLARGVYL
                     APSAYEAGFVSAAHSTAEIEATLAAARAAFARLS"
     gene            2838..3389
                     /locus_tag="VFR57_10365"
     CDS             2838..3389
                     /locus_tag="VFR57_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0259804.1"
                     /translation="MNIPIRELSRLERAALEPHLLALHSDDRRLRFGAPLNDDAVRAY
                     VARIDFERDAAFGVYDDALRLVGAAHVARGNGHAELGVSVLPVNRGCGIGRALLARAH
                     LRARNWGVRALFMHCLTENAAMMHLARMQGMQIVVDSGEADAWLTLPPADASSHFGEV
                     FAQRVALFDYALKQGRAWLGQGR"
     gene            complement(3352..>3598)
                     /locus_tag="VFR57_10370"
     CDS             complement(3352..>3598)
                     /locus_tag="VFR57_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0259805.1"
                     /translation="LWGTLLGAIAYALYGNVALSGVLALAMMLNLVLAALVGVAIPWV
                     RARWGRDPAAGSSVLITGCTDSGGFFIFLGLATLFLV"
BASE COUNT          608 a         1190 c         1190 g          610 t
ORIGIN      
        1 aggtccagtt catcggcaca tcttcccagc gcgtgcccgg tgcgatgcct tcgtcgggca
       61 ggcccgcggc ctcgtcgtac acgaagccgc agatgaggca catccacgtt ctgaactgta
      121 cttggctctc ggtactcata aaatcgagcc ggatattaac ccatgctccc gatagtgctg
      181 acgttcgcgg cgtccgatcc aaccggcggc gccgggctgc aagccgatgt gctcaccctc
      241 gccgccctgg gctgccatcc gctgtcagtt ctcacgggac tcactgtcca ggacacacgc
      301 ggtgtcgacc acctggaacc cattgcggaa gagctcgtga cgcgccaagc gaactgcgtg
      361 ctcgccgaaa gcaaagttgc agcgttcaag gtgggcgtgc tcgcgtccgc agacaacgcg
      421 cgtgccgtgg gtcaggtggc ggccgcgcat gcgcatgtgc cgctcgtact cgacccagtg
      481 ctcgcctctg gccgcggcga cgaattggcg ggcaacgctg ttcaagccgc tttgctggaa
      541 tggcttgtcc cgcgcgccac cgtggtcacg ccgaacactg tggaggcgca gcgccttggc
      601 ggtgtgaagc atctgctcga gcgcggctgc cgttacgtgc tcgttacggg aacgcatgaa
      661 ccgagcgcgg aagtaaccaa caagctctac ggcccgggag gcctcgtacg cgaggaccgc
      721 tggcagcgac ttccggagct cttccatggc tcgggctgca cgctcgcgtc cgcgatcgcg
      781 gcgcagctcg cgaaggggcg cgccgtctcc gaggcggtgc gtgaggcaca agaatttacc
      841 tggcgcgcgc tggcagcagg ttttcgcagc ggcgccgggc agtggctgcc caaccggttc
      901 tttccaagat aatgcgcggg ctctacccta ttactccgaa cacggccgac accgacgcgc
      961 tgctgcgtaa ggtggaggag gcgctccggg caggagtcgc gatgctgcag taccgcaaca
     1021 agtcgatctc caaggataag cgcctgctgc aggcgaagga gctcgctccg ctcgcgcgcg
     1081 gctatggcgt accgctcatc gtgaacgacg acgtggagat cgccctcgca gtaggcgcga
     1141 acggcgcgca cctcggcgag gacgacggcg atcttgccgc cgcgcgcgtc aagctggtcg
     1201 gaaagatact cggggcgtcg tgctacgaca gcctggaaag cgcccggacc gcagtgcgct
     1261 cgggcgccga ctacgttgct ttcggggccg tctacccgtc gcccaccaag cccgatgcga
     1321 agcgcgctcc gctttcgctt ttcggccacg acctcggcgt gccgctttgt gcgatcgggg
     1381 gcatcaccct cgagaacgcg ccggcagtga tcgcggccgg cgcgagcctc ctcgccgtta
     1441 tcagcgacat tttcgacgcg cccgacatcg gtgcgcgggc cagtcaatac cgcaagcttt
     1501 tctcatgaca agaagcaagg acctcttcga gcgtgcccag gcgcgcattc ctggcggcgt
     1561 caactcgccg gttcgcgcgt tccaggcggt cggcgggacg cccgtattct tcgagcgcgc
     1621 ctcgggccct tacctgtggg acgtggacgg caagcgctac atcgactatg tcggctcgtg
     1681 gggaccaatg ctcgccggcc acggccatcc cacggtggtc gaggcggtgc aggaagccgc
     1741 gtcgcgagca ctctcgttcg gagcgccgac cgaggctgag atcgatctgg ctgcgctgct
     1801 ttgccgcgag ctgccctcgc tcgagctggt gcgactggtg tcgtcaggaa ccgaagcgac
     1861 catgaccgcc ttgaggctcg cgcgcggctt caccggccgc agcctgatcg tgaagtttga
     1921 gggctgctat cacggccacg ccgacgcgct tctcgtcaag gcgggctcgg gcgccctgac
     1981 cttcggcaac ccgagttctg ccggcgtgcc cgcgcaggtc gcggcgcaga cggtggtgct
     2041 ggactataac gacgtctatc aggtccagaa cctcttcgca gaaaaaggcc aggaaatcgc
     2101 ggcggtgatc gtcgagccgg tcgcaggcaa catgaatctg gtgcttccgg cgcccggctt
     2161 cctggaggcg ctccgcgaag cgtgcacgcg acacggcgcg gtgctgatct tcgacgaagt
     2221 gatgaccgga tttcgcgttg ccgctggcgg cgcgcaacag cgcttccgca tccgaccgga
     2281 tctcaccacg ctcggcaagg tgatcggcgg cggactgccg ctcgccgccg tgggcggccg
     2341 gcgcgacatc atggagagaa tcgcgccgct cggaccggtc tatcaggcag ggaccctctc
     2401 cggcaaccca atcgccgtgg cggcggggct tgccaccgtg aggctcgtta cgcaagaggg
     2461 gtttcacgag aaggtgcagc gcacgaccgc tgaattggtg catggcctct cggtagaagc
     2521 gaagaaagcg ggaatcgttt tctgcgcgca gtcgatcggc agcatgttcg gtatctactt
     2581 ccgggcggtc ccgccgacga gcttcgccga agtgatgcaa tgcgatcgtg cgcgcttcaa
     2641 tcgcttcttt catgagatgc tcgcgcgagg ggtatatctc gcgccgtccg cgtacgaggc
     2701 gggattcgtg tcggccgcac acagcacggc cgaaatcgaa gctacgctcg cggctgcacg
     2761 ggcggcattc gccagattgt cttaacagaa ctggcgtaaa ccttgcgtcg ttcgcgcggt
     2821 ctaaagcgcg agccgcaatg aatattccga taagggagct gtccaggctc gagcgcgcag
     2881 cgctcgagcc gcatcttctt gcactccaca gcgacgaccg caggctccga tttggtgcgc
     2941 cgctgaatga cgacgcagtg cgcgcctacg tcgcacgcat cgatttcgag cgcgacgccg
     3001 ctttcggcgt gtatgacgac gcgctgcgcc tcgtcggagc ggcgcacgtg gcacgcggca
     3061 atggccacgc cgagctcggc gtgtccgtgc ttccggtgaa tcgcgggtgc ggaatcggcc
     3121 gggcgctact tgcccgtgcg cacctgcgcg cccgcaactg gggcgtgcgc gcgctcttca
     3181 tgcactgcct gacggagaac gcggcgatga tgcacctcgc tcgcatgcaa ggcatgcaga
     3241 tagtagtcga ttctggcgag gccgatgcgt ggctcacgct cccgcccgcc gacgcttcga
     3301 gccatttcgg cgaggtcttc gcgcagcggg tcgcgctctt cgactacgcc cttaaacaag
     3361 gaagagcgtg gctaggccaa ggaagatgaa gaagccgccc gaatcggtgc agccggtgat
     3421 cagcactgag gagccggccg ccggatcgcg cccccagcgc gcgcgcaccc acggaatcgc
     3481 cacgcccaca agcgcggcga gcacgaggtt cagcatcatg gcgagggcca gcaccccgct
     3541 cagcgcgaca ttgccgtaca acgcgtaggc gatcgcgccg agcagcgtgc cccacagc
//
LOCUS       DASTPD010000311         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_782303, whole genome shotgun sequence.
ACCESSION   DASTPD010000311 DASTPD010000000
VERSION     DASTPD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782303"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFR57_10375"
     CDS             complement(<1..347)
                     /locus_tag="VFR57_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEU0259806.1"
                     /translation="MHLLYEEDGELKAGTVLERSPASFHVQSPHGRRSKIKAANVLLA
                     FERPEPAVLLDEAESFSRGLETDFLWECSGAREFGFEELAREYVGRKPTAAEAAGVLM
                     KLHSAPMYFYRRG"
     gene            complement(331..891)
                     /locus_tag="VFR57_10380"
     CDS             complement(331..891)
                     /locus_tag="VFR57_10380"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEU0259807.1"
                     /translation="MLVYLHGFNSSPQSHKAQVLQSYMAGRGLAHEYACPALPPLARD
                     ALRAIAPLLWKNPCFVGSSLGGYYATHLAEKHGLKAVLINPAIDPHVGLRAFLGPQKN
                     LHTGEPYELTEAHLRDWERLYVPRITPGRYLLMVETGDEVLDYRRAVERYAGAEQVVI
                     PGGDHSLQSFPQHLERILEFSRAPSL"
     gene            complement(866..2224)
                     /gene="mpl"
                     /locus_tag="VFR57_10385"
     CDS             complement(866..2224)
                     /gene="mpl"
                     /locus_tag="VFR57_10385"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEU0259808.1"
                     /translation="MAAHLHILGICGTFMGGIAAIAKAAGYRVTGCDANVYPPMSDQL
                     RALGIELTEGFGAEQLELGPDVWVIGNVVTRGNPLMEAILDAGERYVSGPQWFADEVL
                     AGKHVLAVAGTHGKTTVSAMLAWVLERAGQAPGFLIGGVPNDFPVSARLTSSPLFVIE
                     ADEYDTAFFDKRSKFVHYRARTAVLNNIEFDHADIFDDLASIERQFHHFVRTVPRSGR
                     IIANGADTSVKRVLTLGCWTPVEAFGVPDAWQAGNADADGAFEVSFAGSSLGRVRWSL
                     LGAHNRTNALAALAAARHVGIDPAAAIEALGSFGGVRRRMQVRGRASGVTIYDDFAHH
                     PTAIRTTLEGLRDRVGKARILAVLEPRSNTMKRGVMKDLLPPSLAEADRVYVYSSGLG
                     WDARSLFALFGGRARCEDDLDSLIGAVAAEARAGDHVVIMSNGGFGGIHEKLLARLSA
                     RL"
     gene            2287..2868
                     /locus_tag="VFR57_10390"
     CDS             2287..2868
                     /locus_tag="VFR57_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259809.1"
                     /translation="MRARIAATAARIMAEDGIDDFALAKRKAARQLGAAETEALPAND
                     EVEAELRAYRALYQPEEHPLRISELRRIALDAMRALENFHPYLTGPVLKGTAGRYAEI
                     ELQLFPESAKEVEIFLLERRIAYATHEGRRFSGDRAHAMGVLSLAWQGAPLKLSVFDP
                     RDERLALKTSQAGRVMDRAGVAEVEALLRQGQS"
BASE COUNT          530 a          954 c          903 g          482 t
ORIGIN      
        1 ctcccccgcc ggtagaaata catcggcgct gaatgcagtt tcatcagcac ccccgccgcc
       61 tccgccgccg tgggcttgcg cccgacgtac tcgcgcgcaa gctcctcgaa gccgaactcg
      121 cgcgcgccgc tgcattccca cagaaaatcc gtttcgagtc ccctggagaa gctttccgcc
      181 tcgtcgagga gcacggcggg ctcgggccgc tcaaaggcaa gcagtacgtt ggcggccttg
      241 attttcgacc ggcggccgtg cggactctgg acgtggaaag acgccggcga gcgctcgagc
      301 accgtgccgg ctttcagctc gccatcttcc tcatagagaa ggtgcacgtg aaaactcgag
      361 tatgcgttcg agatgttgcg gaaagctctg caggctgtgg tccccgccgg ggatcaccac
      421 ctgctcggct cccgcgtagc gctcgaccgc ccgccgatag tcgagcacct cgtcgccggt
      481 ttcaaccatc aacaagtagc ggccaggcgt aatgcgcggc acatagagcc tttcccagtc
      541 gcgaaggtgc gcttcggtca gctcgtacgg ttcgcccgtg tgcaggttct tctgcggtcc
      601 gagaaaggcg cgcaggccga catgtggatc gatcgccggg ttgattagca ccgccttgag
      661 cccgtgcttc tccgccagat gggtcgcgta gtagccgcca agcgaggagc ctacgaagca
      721 cggattcttc caaagcaacg gcgcgattgc acgcagagcg tcgcgcgcta acggcggcag
      781 ggcgggacag gcgtattcat gcgcgaggcc gcgcccggcc atgtagcttt gcaggacctg
      841 cgccttgtgc gactgcggcg aggaattaaa gccgtgcaga tagacgagca agcagtttct
      901 cgtgaattcc gccgaagccg ccgttggaca taatgactac gtggtcccct gccctcgcct
      961 ccgcggcgac cgcgccgatc agggagtcga ggtcgtcttc gcaacgcgcg cgccccccga
     1021 acagcgcaaa aagcgagcgc gcgtcccagc cgaggcccga gctatagaca tacacgcggt
     1081 cggcctcggc caggctcggc gggagcaagt ccttcatcac gcctcgcttc atggtattcg
     1141 agcgcggctc aagaaccgcc aaaatccgcg ccttgcctac ccgatcccgc aacccctcga
     1201 gggtggtgcg aatcgcagtg ggatggtggg cgaaatcgtc atagatggtt accccgctcg
     1261 cgcgtccccg gacttgcatg cgccgccgca ctccgccaaa ggagcccagc gcctcgatgg
     1321 ccgccgccgg gtctatcccg acgtgtcgcg ccgcggcgag cgcggccagc gcgttcgtcc
     1381 tgttgtgcgc gccgagtagc gaccagcgca cccggccaag ggaggagccg gcgaaactga
     1441 cttcgaaggc cccgtccgcg tccgcgttcc cggcctgcca ggcgtctggc acgccgaagg
     1501 cctcgacggg cgtccagcag ccaagggtga gcactcgctt tacgcttgtg tctgctccgt
     1561 tggcgatgat ccttccagag cgaggcaccg ttcgcacgaa gtggtggaac tgtcgctcaa
     1621 tcgaagcaag atcatcaaaa atgtcagcgt gatcgaactc gatgttgttc agtacagccg
     1681 tgcgcgcccg gtagtgtacg aacttcgacc gtttgtcgaa aaaggccgtg tcgtactcat
     1741 ccgcctctat aacgaagagg ggagacgacg tgagacgggc agatacgggg aagtcgttgg
     1801 gcaccccgcc gatcaagaac cctggtgctt gccccgctcg ctccaagacc caggcaagca
     1861 tcgccgaaac cgttgtcttt ccgtgggtcc cggcgacagc gagtacatgc tttcccgcca
     1921 agacttcatc ggcgaaccac tggggtcccg agacgtagcg ctcacccgca tccaggatcg
     1981 cctccatcag cggattgccg cgcgttacca cattgccgat cacccaaaca tccgggccga
     2041 gctccagctg ctcggcgcca aacccctcgg tgagctcgat gccgagcgca cgcagctgat
     2101 cgctcatcgg gggatacaca ttggcgtcgc agccggtcac gcggtagccg gcagccttgg
     2161 cgattgccgc gattccgccc atgaacgtgc cgcagatgcc caggatatgg agatgcgccg
     2221 ccatgaagcg acgagattct atgattgaat ccagacgtgg gccgcaaagg cactcgccag
     2281 accgcaatgc gcgcgcgcat tgcggcgacc gccgcgcgca tcatggccga ggacggcatc
     2341 gacgatttcg cgctcgccaa gcgcaaggcg gcgcgccagc ttggggcggc cgagaccgag
     2401 gcgctgccag cgaacgacga agtcgaggcc gagttgcgcg cctaccgagc gctgtatcaa
     2461 ccggaggagc atccactgcg catctcggaa cttcggcgca tcgcgctcga tgcgatgcgc
     2521 gccctcgaga acttccaccc gtatctcacc gggccggtgc taaagggaac ggccgggcgc
     2581 tacgcggaaa tcgagcttca actttttccg gagagcgcga aggaggtcga aatcttcctg
     2641 ctcgagcgcc gcatcgcgta tgcgacgcac gaaggtcggc gcttttcggg cgaccgcgcg
     2701 catgccatgg gcgtgttgtc gctcgcctgg cagggcgcgc ctttaaagct ttccgtgttc
     2761 gacccgcgcg acgagcgctt ggcgctcaag acgtcccaag ccgggcgggt aatggaccgc
     2821 gctggcgttg ccgaagtgga agcgctgctg cgccagggcc agtcctaac
//
LOCUS       DASTPD010000312         5270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_577616, whole genome shotgun sequence.
ACCESSION   DASTPD010000312 DASTPD010000000
VERSION     DASTPD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577616"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFR57_10395"
     CDS             <1..521
                     /locus_tag="VFR57_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259810.1"
                     /translation="ILKESLPSATSLAVMYAPRANIEIDLAEAQDTARSTGLALQLFE
                     LKTPQDFERTFDAIARARPSGVMTIGHGMLLGNRGRIVEFCLKAKLPGVFPEREFAEA
                     GGLMTYGPDLAANFRRVGAYVDRIVKGARPGDLPIEQPTKWELVVNLRTAQALGIKIP
                     GTVLVRADALIE"
     gene            complement(523..1218)
                     /locus_tag="VFR57_10400"
     CDS             complement(523..1218)
                     /locus_tag="VFR57_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259811.1"
                     /translation="MIELERINTFYGLGHILHDLSLRVGESEVVALLGRNGAGKTTTL
                     RSITGLTPPRTGAIRYKGRDIAGLDAHHVSRLGIALVPETRDIFSYLSAKENLSIAAR
                     AGSRWNMENVLERFPSLKTRLNNKGRELSGGEQQMLAIARALLTGPDLLLLDEPSQGL
                     APLVVNAVMSTIDELKKERVSMLLVEQNAEMALRLADRVYVIDHGTVVFEGSPAALRA
                     DTQVTRTYLGVGG"
     gene            complement(1215..1982)
                     /locus_tag="VFR57_10405"
     CDS             complement(1215..1982)
                     /locus_tag="VFR57_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259812.1"
                     /translation="MVKVLGMAILETQNLGKSFGALTAVKDVSLSIEAGSLHSIIGPN
                     GAGKTTLFNLLTGTFAPTTGRIFYNGQDITGTRAHRIAHLGLARSFQRTNVFPAFSLF
                     DNVWVAAFATARSWQGLIYRKAAAYPEAAERARQALSDVGLLPKADVPAKAISHGEQR
                     QLELAIALAAAPRVLLLDEPAAGLSPEETRRMVALVRALKGRYTIVLIEHKIDIIMSV
                     SDRISVMHFGSLIAEGPPAEIQRNPDVRRAYLGGIAA"
     gene            complement(1916..2860)
                     /locus_tag="VFR57_10410"
     CDS             complement(1916..2860)
                     /locus_tag="VFR57_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259813.1"
                     /translation="MRALVLILVSICVLPFIVRPAIATEIWIFAIFGLGLNLLLGYTG
                     LLSFGQSTFFGSAAYVAGWLLKHYGINVFIALGIGVGVGALSALLVGYLCVQRSGLYF
                     IMLTFALNQLFYFIAYQWTTVTGGEDGMPGIPRPDFLGLNFKDPLVYYVFVSVLFLLS
                     LWAMKRIVESPLGKILQGIRENEVRAEAVGYNVPRFKLLAFVIGGAFSGLAGVLYAML
                     FGIVPLEAISFVFSGNVVFATLIGGSGSLYGPIIGSFVFIWLSESMSVVWARWPLLLG
                     VAFVIVVLFLRGGVVEAWSRFWAWQSSRHKTSANPSAR"
     gene            complement(2857..3714)
                     /locus_tag="VFR57_10415"
     CDS             complement(2857..3714)
                     /locus_tag="VFR57_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259814.1"
                     /translation="MNLLLGQILNGIATGMLYALMGLGLSLITGVLNIPNFAHGALFA
                     VGAYFLVTVANAVGNFWIGLLLAPLGVAVLGLIIEYAGVRRLYAAGHDYQLLFTFGLA
                     LILNELIILIWGPVGISHLPPALLRGGVDLGFTFYPKYRLFVMVAAGLLVLATWLFLE
                     KTRYGAIMRAGIENKEMVSLLGIDIHRLFTVAFATGAFLAGIAGALTAPIRGLSPGMG
                     VDMLGIAFVVVALGGLGNLLGAIAAGLLIGVAQAVVAAYWTEASTAVIFAVMALVLLI
                     RPQGLFGIR"
     gene            complement(3718..4923)
                     /locus_tag="VFR57_10420"
     CDS             complement(3718..4923)
                     /locus_tag="VFR57_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259815.1"
                     /translation="MKRRDILKAGAAAAGLGFPAIIRAQQKEVVILGLWDQTGAFADV
                     GPLNDRGMRMALEERKMQILGRPVRYITRDGATQAGTSTRRAEEAIDGEGARFMIGPW
                     SSGVALAVSEVAKRKKVLHFFSGGTEDISGSRCHRYSFQWAASPYTAAKTVVDNFMKA
                     NPKARRWHLLVADYAFGWSVEKYIKEVGKGHGIEFVGADRHPLGEREFSNYVQKAAAN
                     RPDVVAMINFGGDAVAGAREIFNFGLTPKIPLIMAWSSGVEELIQLAPEIRDNLWVGT
                     NFYYTADTPVAKEFVKNYRARFKTPPGYAPSAAYSMTRLMLAGFDRAKSVEVQDVIKA
                     LEGIEVDDLLGRMRVDPATHQTLRPYFFLRCKPKKAMKEELDFADVVATGDTPLPKEY
                     ATCKDIGAF"
     gene            5017..5244
                     /locus_tag="VFR57_10425"
     CDS             5017..5244
                     /locus_tag="VFR57_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259816.1"
                     /translation="MTRMTATLGVVLLLGVGPAGAQSDSLKESFKKTEKGAGELFRGM
                     GQELKKFGGTLIGGTKKDAKKEAAKADEKEK"
BASE COUNT         1019 a         1614 c         1806 g          831 t
ORIGIN      
        1 agattctcaa ggaaagcctg ccgagcgcga cgagcctggc cgtgatgtac gcgccgcggg
       61 ccaacatcga aattgacctg gccgaggcgc aggacaccgc ccgctcaacg gggcttgcac
      121 tgcagctctt cgaattgaaa actccgcagg acttcgaaag gacgttcgac gccatcgcgc
      181 gcgcccggcc gagcggcgtg atgaccatcg gccacggtat gttgctcgga aaccgcgggc
      241 gcatcgtcga gttctgcctg aaggccaagc tgcccggagt tttcccggag cgcgagttcg
      301 cggaagcggg aggtctgatg acatatgggc ccgatctggc ggcaaacttc cgccgcgtcg
      361 gcgcttatgt ggaccggatc gtgaagggtg cacgcccggg cgatctaccg atcgagcagc
      421 cgacgaaatg ggagttggtc gtcaacctgc gcacggccca ggcgctcggc atcaaaatcc
      481 cgggcacggt gctggtccgc gccgacgcgc tcatcgagta gctcagccgc cgacgccgag
      541 gtaggtcctc gtgacctgcg tgtccgcgcg cagcgcggcc gggctgcctt cgaagaccac
      601 ggtgccgtga tcgatcacgt agacgcggtc ggcgagccgc agcgccatct cggcgttctg
      661 ctcgacgagc agcatgctga cgcgctcttt cttgagctcg tcgatcgtgc tcatcaccgc
      721 gttcaccacg agcggtgcga ggccttggga aggttcgtcg agtagcagca gatccgggcc
      781 ggtgaggagg gcgcgcgcga tggccagcat ctgctgctcg ccgccggaaa gctcgcgccc
      841 tttgttgttg agccgggtct tgaggctcgg gaagcgctcg agcacatttt ccatgttcca
      901 gcgggaaccg gcgcgcgcgg cgattgacag gttctccttc gcggagaggt aggaaaagat
      961 gtcgcgcgtc tcaggcacga gcgcgatgcc caggcgcgag acgtggtggg cgtcgaggcc
     1021 ggcaatgtcg cggcccttgt agcggatggc gcccgttctc ggcggcgtca ggccggtaat
     1081 ggagcgcagc gtcgtggtct tgcccgcgcc gtttcggccg agcagcgcca cgacctcgct
     1141 ttcgccgacg cgcagcgaga gatcgtgcag gatgtgcccg aggccgtaga acgtattgat
     1201 gcgctccagc tcgatcatgc ggcgatgccg cccaggtagg cgcgccgcac gtccgggttg
     1261 cgctggatct ccgccggcgg gccttcggcg atgagcgagc cgaagtgcat gaccgagatg
     1321 cggtcggaca cgctcatgat gatgtcgatc ttgtgctcga tgagcacgat cgtgtagcgg
     1381 cctttcaagg cgcgcacgag cgccaccatg cggcgcgtct cctctggcga aagaccagcc
     1441 gcgggctcgt ccaggagcag aacgcgcggc gcggcggcga gggcgatggc gagctcgagc
     1501 tggcgctgct cgccgtggga gatggctttc gcgggcacat cggccttcgg gaggaggccc
     1561 acgtcagaaa gcgcctggcg tgcgcgctcg gcggcctcgg ggtaggcggc cgccttccta
     1621 tagatgagac cttgccacga gcgggccgtg gcgaaggcgg ctacccagac attgtcgaag
     1681 agagaaaacg ccgggaagac gttggtgcgc tggaatgagc gcgctaggcc gaggtgcgcg
     1741 atgcggtgcg cgcgcgtgcc ggtgatgtcc tggccgttgt agaagattcg cccggtggtc
     1801 ggtgcgaacg tgccggtgag aaggttgaag agcgtggtct tgccggcgcc gttcgggccg
     1861 atgatcgaat gcaggctgcc cgcctcgatg gacaggctca catccttgac ggcggtcagc
     1921 gcgccgaagg atttgccgag gttttgtgtc tcgaggattg ccatgcccag aaccttgacc
     1981 acgcttccac caccccgccg cgcaagaaca gcacgacgat gacgaacgcc acgccgagga
     2041 ggagcggcca gcgcgcccac accacgctca tcgattcgga aagccagatg aacacgaacg
     2101 agccgatgat cggcccgtag agcgacccgg agccgccgat cagcgtcgcg aacaccacgt
     2161 tgccggaaaa cacgaagctg atggcctcga gcggcacgat gccgaagagc atggcgtaga
     2221 gcacgccggc gagcccggag aacgcgccgc cgatcacgaa ggcaagcagc ttgaagcgcg
     2281 gcacgttgta gcccaccgct tcggcgcgca cttcattctc gcgaatgccc tgcaggattt
     2341 tgccgagcgg cgattcgacg atgcgcttca tcgcccacag ggacagcagg aacagaaccg
     2401 agacgaagac gtagtagacg agcgggtctt tgaagttgag gccgaggaag tcgggacgcg
     2461 gaatgcccgg catgccgtcc tccccgcccg tcactgtggt ccactggtag gcgatgaagt
     2521 agaagagctg gttcagtgcg aacgtgagca tgatgaagta gaggccggag cgctgcacgc
     2581 acaggtagcc gacgagcagc gcggaaagcg cgcccacgcc gacgccgatg ccgagcgcaa
     2641 tgaacacgtt gatgccgtag tgcttgagaa gccagccggc gacgtaggcg gccgagccga
     2701 aaaaggtgga ctggccgaac gagagcaggc ccgtgtagcc aagcagcagg ttaagcccca
     2761 ggccgaagat cgcgaagatc cagatctcgg tcgcgatcgc cggccgcacg atgaacggca
     2821 gcacgcagat cgacaccaga atgaggacga gcgctctcat cggatgccaa agagcccttg
     2881 cgggcggata aggagcacga gcgccatgac cgcgaaaatc accgcggtcg acgcttccgt
     2941 ccaatacgcg gcgacgacag cttgcgcgac gccgatgagc agtcccgcgg cgatcgcgcc
     3001 gagcaggttg ccgaggccgc cgagcgcgac caccacgaac gcaatgccga gcatgtcgac
     3061 gcccatgccg ggcgaaaggc cgcggatcgg cgcggtgagc gcgccggcaa tgccggccag
     3121 gaatgcgccg gtcgcaaacg caaccgtgaa caggcgatgg atgtcgatgc cgagcagcga
     3181 caccatctcc ttgttctcga tgcccgcgcg catgatcgcg ccgtaacgcg tcttctcgag
     3241 gaagagccag gtggcgagga caagaaggcc ggctgccacc atcacgaaga gccggtactt
     3301 cggatagaac gtgaagccga gatcaacgcc gccacgcaag agcgccggcg gcagatggct
     3361 gatgccgacc ggtccccaga tcaggatgat cagctcattc aggatgagtg cgaggccgaa
     3421 ggtgaagaga agctggtaat cgtgacccgc ggcgtaaagc ctgcgaacgc cggcgtattc
     3481 gatgatgagg ccgaggactg cgacgccgag cggggcgagc aagaggccga tccagaagtt
     3541 gcccaccgcg ttggcgaccg tgacgaggaa ataggcgccc acggcgaaga gcgcgccgtg
     3601 ggcgaagttc gggatgttga gcacgcccgt gatgagcgag agacccaggc ccatcagggc
     3661 atacagcatc cccgtcgcga tcccgttgag gatctggccg aggagtagat tcaaaagcta
     3721 gaaggcgccg atgtccttac aggtcgcgta ttccttgggc agcggggtat cgcccgtggc
     3781 cacgacgtcg gcgaaatcga gctcctcctt catcgccttt ttcggcttgc agcgcaggaa
     3841 gaagtacgga cgcagcgtct ggtgcgtcgc cgggtccacg cgcatgcggc cgaggaggtc
     3901 gtccacctcg atgccctcga gcgccttgat cacgtcctgc acttccacgg acttcgcgcg
     3961 atcgaagccg gcgagcatga ggcgtgtcat cgagtaggcg gcggaaggcg cgtatcccgg
     4021 cggggtcttg aagcgcgcgc gatagttctt cacgaactcc tttgccaccg gcgtgtcggc
     4081 ggtgtagtaa aagttcgtgc cgacccagag gttgtcgcga atctccggtg cgagctgaat
     4141 cagctcctcg acgccggagg accacgccat gatgagcgga atcttcggcg tcaggccgaa
     4201 gttgaagatc tcgcgcgcgc cggccactgc gtcgccgccg aaattgatca tggcgacgac
     4261 atccggacgg ttcgccgcag ccttttgcac atagttggag aactcacgct cgccgagcgg
     4321 atggcgatcg gcgccgacga actcgatgcc gtggcccttg ccaacttcct tgatgtattt
     4381 ttccaccgac cagccgaagg cgtagtcggc gacgagcagg tgccagcgcc gcgccttcgg
     4441 gttcgccttc atgaaattgt cgaccacggt tttcgccgcg gtgtacgggc tcgccgccca
     4501 ctggaacgag tagcgatggc agcgcgaccc ggagatgtcc tcggtgccgc cgctgaagaa
     4561 atgcagcact ttcttgcgct ttgccacttc ggagacggcc agggcgacgc ccgaagacca
     4621 cgggccgatc atgaagcgcg cgccttcacc gtcgatcgct tcttcggcgc ggcgcgtgga
     4681 agtgccggcc tgcgtggcgc cgtcgcgcgt gatgtagcgg accggccgtc cgaggatctg
     4741 catcttgcgc tcttcgagcg ccatgcgcat gccgcggtcg ttgagcgggc cgacgtcggc
     4801 gaacgccccg gtctgatccc agaggccgag gatcacaacc tccttctgct gcgcgcgaat
     4861 aatggcggga aatccaaggc ccgccgccgc cgctccggcc ttcaggatat ctctgcgttt
     4921 catgtgcctc ctccctcgtg aaggcgcgag cgtaccgcaa actgttcgcc ggcggtcgaa
     4981 ctgttccggc agatcggcca gtgtaggatt gcgcgcatga cacggatgac agccacgctt
     5041 ggcgtcgtgc tgctccttgg cgtcgggccc gccggagctc agagcgacag cctcaaggag
     5101 agcttcaaga aaaccgagaa aggcgccggg gagcttttcc gagggatggg gcaggagctg
     5161 aaaaaattcg gcggcacgct cattggcgga acgaagaaag acgccaagaa ggaagcggcg
     5221 aaagcggacg agaaagagaa ataggttgac cgtccggttg atcgcttcgg
//
LOCUS       DASTPD010000313         8719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1803860, whole genome shotgun sequence.
ACCESSION   DASTPD010000313 DASTPD010000000
VERSION     DASTPD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803860"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFR57_10430"
     CDS             complement(<1..557)
                     /locus_tag="VFR57_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259817.1"
                     /translation="MEQALRSPFWQKRIPNNIALERLDNPDVWRSIPILDKETLRALT
                     DAEFYAEFCVSGDDGIAEYWRSGGVTGQPLFYPRSFRDIEYGLEAFARTFDCAGAKPG
                     DRAHVSFPMGIHPVGQVFARCAGSRGITVNWAGAGTSTPSALQIELIRRLKPTIWLGM
                     SSYALHLANLAEARGLELRIDKLMTS"
     gene            680..1108
                     /locus_tag="VFR57_10435"
     CDS             680..1108
                     /locus_tag="VFR57_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267470936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEU0259818.1"
                     /translation="MRAILAALLLALACGVQAQNNVDSSGLALKGYDPVAYFTEGKTV
                     QGKPEFTARHEGATYRFASAANRDAFVAGPAKYAPQYGGYCAYGMASGYKAPIEPDAW
                     TIVDGKLYLNYNRSVRDRWSSDIPGSIRKADANWPSVRSK"
     gene            1121..2155
                     /locus_tag="VFR57_10440"
     CDS             1121..2155
                     /locus_tag="VFR57_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259819.1"
                     /translation="MSRALRRGTPLASPVTSSEVKRRARELGADLCGIASAATLNAFP
                     PDPRWPQTPERISPYCKSVVVIVQRIPVGAFRCKTNVPVQYLDMLVLRRMDRIALKLA
                     QELERAGHPSFTLAAQETVWDMKRASYGQLSTRHLGVEAGLGTLGLEVNILTPEFGPR
                     LYLTGILSELELEADRRMEEQVCIGESCSRCLHSCPPDAVRQWGLDKAGCATEAQEFG
                     FMQITRFFSKYFDLSQEEKKAAVRSRDLFGFWQGLLRVVGSFGDCPRCLAVCPVGNDY
                     HAHLADIQKQIPEKTAEKVAKAAAFKQARKSGVELPGLSQWNIRWVGEKGYQGIVARQ
                     LQEFKKRQQQ"
     gene            2152..3108
                     /locus_tag="VFR57_10445"
     CDS             2152..3108
                     /locus_tag="VFR57_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259820.1"
                     /translation="MNILKRKLTAEEIKAKARELGADLVGIADGSKLNTKEITEHDGG
                     RVIMLAVRLQAGTSRIRAWNDRNKFYNDELSLTFLEEVSLELVYWLEDAGYPAIIVPP
                     THVDPWRYDGDPKKHQRTLLSLPHAAVEAGLGSLGLNLQLLTPQFGPRVLLTAVLCSV
                     DVECDKPTTEALCLGPECGRCLKTCPADAVLEWDRDWEACDRYRSPHGFAQLSEYLGR
                     VIDEPDAARKKAMLRSEASFNLWQSVLRGAGVITGCRRCADVCPVGTDYEAMLKDALE
                     AIPENTPHKEARVAAMAKGESSAAYQRQVRWIGRMNSRRRDS"
     gene            3105..4277
                     /locus_tag="VFR57_10450"
     CDS             3105..4277
                     /locus_tag="VFR57_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259821.1"
                     /translation="MKMRSAVLAAAAAFACSAHAANELKIGYLSTLSGPAGAIGVEIR
                     DGFNLALKMAGGKLGGLPTEVVFADDTLNPETGKQLAERLIKRDRVNLMTGVVFSNVM
                     LAVWPTIQAGKIFYIAANATPTRISGEGCNPHFVSASWPNEGYHEAAGHYAKSKGYKN
                     AYLIAPNYPAGKDATSGFKRGFGGTIAAEVYTKLNQLDYSAELAQIRAAKPAALYAFL
                     PGGMGINFIKQFVAAGLSSEVPLVVPGFVSDQDIVRAVGEPMLGIFDTAHWSYDLDND
                     ANRKFVSAFEKEYNRIPSVFAEQGYTTALLIDTAVRSVKGKIEDEKGFHKALMAAPAR
                     FKSPRGDWKFAPNGTPVQDYYLRVLAKDNQGRIVNKKIGTVMNAHVDFWAKECKMK"
     gene            4359..5279
                     /locus_tag="VFR57_10455"
     CDS             4359..5279
                     /locus_tag="VFR57_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259822.1"
                     /translation="MTPILLLEQCLNSLQFGLMLFLLAAGLTLVFGIMDMINLAHGSL
                     YMIGAYLMATFAEATGSFWAALPLALVATALVGAALEVSLLRKLYERDHLTQVLATFA
                     VILIANEVVRIIWGNQSLMLNPPSTLTGPVQLPGGMQYASYRLAIIAVGAAVAVCLYL
                     LVARTKMGMLVRAGASNREMAIAMGVNIRRLFTVVFAFGAALCALAGAMLGPILAVQV
                     GMGENILILAFVVIVIGGIGSIRGAFVGALLVGLVDTMGRALLPIAFAKMFSPQVASN
                     LGPSISSILIYVLMAAVLFLRPQGLFPARG"
     gene            5276..6211
                     /locus_tag="VFR57_10460"
     CDS             5276..6211
                     /locus_tag="VFR57_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0259823.1"
                     /translation="MKRILFIAVLACAFPWAMQALDQHFYTSFATRILIYAMAAASLN
                     LVLGYGGMVSFGHAAFFGAGAYIVGILAVEGITSLWISWPLAIAVAALAAAAIGAISL
                     RTRGVYFIMITLAFAQMMYYVFVSLKAYGGDDGLSLPGRSSGLGVSLSDDFVWYYLVL
                     VLLIVILYLLHRLLASRFGRVIEAIRENETRATAVGYPVYRYKLLCFVISGAIAGLAG
                     ALIANQTSYVGPALLHWVQSGTLMIMVILGGVGRFWGGPVGAGVLLALEELFSGSPLL
                     GPYSLHWQLPVGAILLAVVLFAPQGIAGAFAKKPQ"
     gene            6186..6929
                     /locus_tag="VFR57_10465"
     CDS             6186..6929
                     /locus_tag="VFR57_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259824.1"
                     /translation="MLSLRNLSKRFGGVVAVDGVTLDVAAGEVHALIGPNGAGKTTLI
                     HQISGTLSVDVGEIFFLEKDITRLPAHQRVAAGLARSYQITSIFRRFSVLDSLALAVQ
                     ARSGSSFSFWRPVAEEAALFEEARRIAAQVNLTQKLDAVASTLSHGEQRALEVGLALA
                     TRPKLVLLDEPMAGMGPEESKRMIELIGRIRTDVAVLLVEHDMDAVFRLADRISVMVN
                     GRLIATGFPEQIRMNEEVRKAYLGDELAA"
     gene            6922..7623
                     /locus_tag="VFR57_10470"
     CDS             6922..7623
                     /locus_tag="VFR57_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259825.1"
                     /translation="MLEVDNLETAYGTSQVLFGLSFSAAEGEAVTLLGRNGMGKTTTV
                     RSILGLTRARGGAVSFRGRKIEREPPDRIARMGIALVPEGRQIFPNLSVREHLVAFAE
                     NRSGGSDPWTPEKVFQLFPRLGERQNNQGNQLSGGEQQMLAIGRALVTNPHLLILDEA
                     TEGLAPLVREEIWRCLGRLRAAGQTILVIDKYIERLVRLADRHTIIERGRVAWQGSSA
                     ELDADRGLWHRYLGI"
     gene            complement(7848..8057)
                     /locus_tag="VFR57_10475"
     CDS             complement(7848..8057)
                     /locus_tag="VFR57_10475"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259826.1"
                     /translation="MPSGASVAQREAERLASLTFFLDHQIGRYQVAEMLRSAGAKVEV
                     HLDHFPGDMLDVEWIPEVARRGWVP"
     gene            complement(8026..>8719)
                     /locus_tag="VFR57_10480"
     CDS             complement(8026..>8719)
                     /locus_tag="VFR57_10480"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0259827.1"
                     /translation="NAFTDRDLREHPAYSIPEAAGYLRLPVSTLRAWMMGQHYRAGEQ
                     AKFFKPVIEIADRRERQLSFINLVEAFVLSGIRREHDIPLPKVRRAVDYLRRAFQSKR
                     PLADEQFETDGVDLFVEKMGSLIGVSQEGQIQMREIIRGRLKRVQRDPKGVPQRIVLF
                     PAQRDKTKSADVVIDPRLSFGRPVLDQFGVRTAILAERFDAGDDIDVLARDYAAPPEA
                     IQNAIRCERRAA"
BASE COUNT         1508 a         2797 c         2852 g         1562 t
ORIGIN      
        1 gaggtcatca gcttgtcgat gcgaagctcg aggccgcgcg cttcggcgag attggcgagg
       61 tgcaacgcat agctggacat gccgagccag atcgtcggct tgaggcggcg gatgagctcg
      121 atctgcagcg ccgaaggcgt cgaggtgccg gcgccggccc agttgacggt gatgccgcgc
      181 gagcccgcgc agcgcgcgaa gacttgccct accgggtgga tgcccatcgg gaacgacacg
      241 tgcgctcggt cgccgggctt cgcgcccgcg cagtcgaagg tgcgggcgaa ggcttcgaga
      301 ccgtattcga tgtcgcggaa actgcgcggg tagaagagcg gttgtccggt gacgccgccg
      361 gagcgccagt attcggcgat gccgtcgtcg cccgagacgc agaactcggc gtagaactca
      421 gcgtcggtaa gcgcgcgcag cgtctccttg tcgaggatcg gaatgctgcg ccagacgtcc
      481 ggattgtcca gtctctccaa agcaatattg ttcgggatcc ttttttgcca gaagggcgag
      541 cgcagcgcct gctccaccgc ggcgcgccta cggaggcttt ggatgcggcg gatctcctgc
      601 cgggagctgg gaagggacag cgggggcggc accggtggat tctgccgtaa gtccagcgcg
      661 cgcgggccga ccaagcggca tgagagcgat cctggctgcc ttgctcctgg cgctggcttg
      721 cggcgtccag gcacaaaaca acgtcgattc gtcgggtctt gcgctgaagg gctatgaccc
      781 ggtcgcctat ttcaccgagg gcaagaccgt ccagggcaag cccgagttca cggcgcgcca
      841 cgagggtgcg acctaccgct tcgcgagcgc agcgaaccgc gacgccttcg tcgcggggcc
      901 ggccaagtac gcgccgcagt acggcggcta ttgcgcgtac ggcatggctt ccggctacaa
      961 ggcgccgatc gagccggacg cctggaccat cgtcgacggc aagctctacc tgaattacaa
     1021 ccggtcggtc cgcgaccggt ggtcgtccga catcccgggg tccattcgca aggcggacgc
     1081 caactggcca tcggttcgct ccaagtgaga cactgcgcga atgtcccgtg cgttgcgccg
     1141 cggcaccccc cttgcgtctc cagtaacgtc gtctgaagtc aagcgccgcg cccgcgagct
     1201 cggcgccgat ctctgcggca tcgcctccgc cgcgacgctc aacgcgtttc cacccgaccc
     1261 gcgctggccg cagactcccg agcgcatttc gccgtactgc aagagcgtgg tcgtcatcgt
     1321 gcagcgcatc ccggtcggcg cgtttcgctg caagaccaac gtgccggtgc agtacctcga
     1381 catgctggtg ctgcggcgca tggaccgtat cgcgctcaag ctcgcgcagg agctcgagcg
     1441 cgctggccat ccgtcgttca cgcttgcggc ccaggaaacc gtgtgggaca tgaagcgcgc
     1501 gagctacggg caactctcga cgcgccatct cggggtcgag gcgggcctgg gcacgctcgg
     1561 cctcgaagtc aacatcctca cgcccgagtt cggcccgcgg ctctatctca ccggcatcct
     1621 tagcgagctc gagctcgagg ccgaccggcg catggaggag caggtatgca tcggggagtc
     1681 gtgcagccgc tgcctgcact cgtgcccgcc ggacgcggtg cgccagtggg ggctcgacaa
     1741 ggcgggctgc gccaccgagg cgcaggaatt cggcttcatg caaatcacca ggttcttcag
     1801 caagtatttc gatctttccc aggaagagaa aaaagcggcg gtgcgctcga gagatctatt
     1861 cgggttctgg cagggactgc tgcgcgtggt cggctcgttt ggcgactgtc cgcgctgcct
     1921 cgccgtctgc ccggtcggta acgattacca cgcgcacctg gccgacattc agaagcagat
     1981 ccccgagaaa accgccgaga aagtcgccaa agccgcggcg ttcaagcagg cgcgcaagag
     2041 cggcgtcgag ctgcccgggc tttcgcagtg gaacatccgc tgggtcgggg agaagggcta
     2101 ccaggggatc gtggcgaggc agctacagga attcaagaag agacagcaac agtgaacatt
     2161 ctcaagagga agctcaccgc ggaggagatc aaggccaaag cgcgcgagct gggcgccgac
     2221 ctggtcggaa tcgcggacgg aagcaagctg aacacgaagg aaatcaccga gcacgacggc
     2281 ggccgcgtga tcatgctcgc ggtgcggctg caggcgggga cttcacgcat ccgcgcctgg
     2341 aacgaccgca acaagttcta caacgacgag ctgtcgctca ctttcctcga ggaagtgtcg
     2401 ctcgagctcg tgtactggct ggaggacgca ggctacccgg ccatcatcgt gccgccgacg
     2461 cacgtcgacc cttggcgcta cgacggcgac ccgaaaaagc atcagcgcac gctcctctcg
     2521 ctgccgcacg ccgcagtgga ggcaggcctc ggcagccttg gtttgaatct tcagttgttg
     2581 accccccagt tcggcccgcg ggtactgctc accgcggtcc tatgctcggt cgacgtggaa
     2641 tgcgacaagc cgacgaccga agcgctctgc ctcgggcccg agtgcggacg ctgcctcaag
     2701 acctgccccg ccgacgcggt gctcgagtgg gaccgcgact gggaagcgtg cgatcggtac
     2761 cgctcgccgc atggcttcgc gcagctctcg gagtacctcg ggcgcgtcat cgatgagccc
     2821 gacgccgcga ggaagaaagc gatgctgcgc tccgaggcga gcttcaacct ctggcagagc
     2881 gtcctgcgcg gcgctggcgt aatcaccggc tgccggcgct gcgccgacgt ctgtccggtc
     2941 ggcacggact acgaggcgat gctcaaggac gcgctcgagg ccattccgga gaatacgccg
     3001 cacaaggaag cgcgtgtcgc ggcgatggcg aagggcgagt cttcggccgc gtatcagcgg
     3061 caagtacgct ggattggtag aatgaattcg aggaggaggg attcatgaaa atgagatcgg
     3121 ctgtcctggc cgccgcggcg gccttcgcct gcagtgccca tgcggcaaac gagctcaaga
     3181 tcgggtacct gtcgacgctg tccgggccgg cgggggcgat cggcgtggag atccgcgacg
     3241 gcttcaacct cgcgctgaaa atggcaggcg gcaagcttgg cgggctgccg accgaggtgg
     3301 tgttcgccga tgacacgctc aaccccgaga ccggcaagca gctcgccgag cgcctgatca
     3361 agcgcgaccg cgtgaacctg atgaccggcg tggtgttctc gaacgtgatg ctcgcggtgt
     3421 ggccgacgat ccaggcaggc aagatcttct acatcgcagc gaacgccacg ccgacacgca
     3481 tctccggcga gggctgcaat ccgcatttcg tctcggcctc gtggccgaac gagggatatc
     3541 acgaagccgc gggccactac gcgaaatcga aaggctacaa gaacgcctac ctgatcgcgc
     3601 cgaactatcc cgcgggcaag gatgcgacct ccggcttcaa gcgcggcttc ggcggcacga
     3661 tcgcggccga ggtctacacg aaattgaacc agctcgacta ttccgccgag ctcgcccaga
     3721 tccgcgccgc caagcctgcc gcgctgtacg ccttcctgcc gggtggcatg ggcatcaact
     3781 tcatcaagca gttcgtcgcc gccgggcttt cctccgaggt gccgctggtg gtgccgggct
     3841 tcgtctccga ccaggacatc gtgcgcgcgg tcggcgagcc gatgctcggc atcttcgaca
     3901 ccgcgcactg gtcctacgat ctcgataacg acgccaatcg caagttcgtg tccgcgttcg
     3961 agaaggagta caaccgcatc ccgagcgtgt tcgccgagca gggctacacc accgcgctgc
     4021 tcatcgatac ggccgtacgc tcggtaaagg gcaagatcga ggacgagaag ggcttccaca
     4081 aggcgctgat ggccgcgccc gcgcgcttca agtctccgcg aggtgactgg aagttcgcgc
     4141 ctaacggcac gccggtgcag gactactacc tgcgcgtgct cgccaaggac aaccagggcc
     4201 ggatcgtcaa caagaagatc ggcacggtga tgaacgctca cgtcgacttc tgggcgaaag
     4261 aatgcaagat gaagtgagca gaatggggtc agaccccatt ttcggttgct cggcgtagcc
     4321 cgcacgaaaa tgggggctga ccccaaggaa atcgggctct gaccccgatt ttgcttctcg
     4381 agcagtgtct gaacagcctg cagttcgggc tgatgctgtt tctcctcgcc gccgggctga
     4441 cgctggtgtt cgggatcatg gacatgatca atctggcgca cgggtcgctg tacatgatcg
     4501 gcgcctacct gatggcgacg ttcgccgagg cgacggggtc gttctgggcc gcgctgccgc
     4561 tcgcgcttgt cgccaccgcc ctggtgggcg ccgctctgga ggtcagtctc ctgagaaagc
     4621 tctatgagcg cgaccatctt acgcaggtgc tcgcgacgtt cgcagtgatc ctgattgcga
     4681 acgaggtggt gcgcattatc tgggggaacc agtcgctgat gctgaatccg cctagtacgc
     4741 tcacgggacc ggtgcagctg ccgggcggca tgcagtacgc ctcctatcgc ctggctatca
     4801 tcgcggtcgg cgccgccgtc gcagtctgcc tctatttgct ggttgccaga acaaagatgg
     4861 gaatgctggt ccgggccgga gcctcgaacc gagagatggc gatcgcgatg ggcgtcaaca
     4921 tccggcgcct cttcacggtt gtgtttgcgt tcggcgcggc gctgtgcgcg ctcgcgggcg
     4981 cgatgctcgg accgatcctc gcggtacagg tcggaatggg cgagaacatc ctgatcctcg
     5041 ccttcgtggt aatcgtgatc ggcggcattg gctcgatccg cggcgcgttc gtcggcgcgc
     5101 tgctcgtcgg actggtcgac acgatggggc gcgcgctgct gccgatcgcc ttcgccaaga
     5161 tgttctcgcc gcaggtcgcc tccaacctcg gcccgtcgat cagctcgatc ctcatctacg
     5221 tgctgatggc cgcggtgctc ttcctgcggc cccaggggct ctttcccgcc cgcggatgaa
     5281 gcgaatcctc tttattgcgg ttctcgcgtg cgcctttcca tgggccatgc aggcgctcga
     5341 ccagcacttc tatacgagct tcgccacgcg catcctgatc tacgccatgg cggcggcgag
     5401 cctcaacctc gtgttgggct acggcggcat ggtgagcttc ggccacgccg ccttcttcgg
     5461 cgccggcgcc tacatcgtcg gcatcctggc ggtcgaaggc atcacgagct tatggatctc
     5521 ctggccgctc gcgatcgcgg tcgcggcgct cgccgcggct gcgatcggcg caatctcgtt
     5581 gcgcacgcgc ggggtgtact tcatcatgat cacgctcgcc ttcgcgcaga tgatgtacta
     5641 cgtgttcgtc tcgctgaaag cctacggcgg cgacgacggg ctttcgctgc ccgggcgctc
     5701 gagcggcctg ggcgtcagcc tctcggacga cttcgtctgg tattacctgg ttttggtctt
     5761 gttgatagtc attctttact tgctgcaccg gctgttagcc tcgcgcttcg ggcgcgtgat
     5821 cgaggcgatc cgcgagaacg agacgcgcgc caccgcggtc ggctacccgg tgtatcgcta
     5881 caagctgctg tgcttcgtga tctcgggcgc gatcgccgga cttgcgggcg cgctgatcgc
     5941 caaccagacg agctacgtcg gcccggcgct gctgcactgg gtgcagtcgg gcacgctgat
     6001 gatcatggtg atcctcggcg gcgtcgggcg cttctggggc ggcccggtcg gcgcgggagt
     6061 gctgctcgcg ctggaggagc tgttctcggg cagcccgctc ctcggcccct actcgctgca
     6121 ctggcagctc ccggtgggcg cgatcctgct cgccgtggtg ctcttcgcgc cgcagggaat
     6181 cgcaggtgct ttcgctaaga aacctcagta agcgcttcgg tggggtggtg gcggtcgacg
     6241 gcgtcaccct ggatgtcgcc gccggcgaag tgcacgcgct gatcggaccg aacggcgccg
     6301 gcaagacgac actgatccac cagatctcgg gcacgctctc ggtcgacgtc ggtgaaattt
     6361 tctttctcga aaaggacatc actcgcctcc ccgcgcacca gcgggtggct gccggcttgg
     6421 cgcgcagtta ccagatcacg agcatcttcc gccgcttcag cgtgctcgac agcctggcgc
     6481 tggcggtgca ggcgcgcagc ggctccagct tctcgttctg gcggccggtg gccgaggagg
     6541 cggccttgtt cgaagaagcc cgccggattg ctgcgcaagt gaatctcact caaaagctcg
     6601 acgctgtcgc gtcgaccctt tcgcatgggg agcagcgcgc gctcgaagtc gggctcgcgc
     6661 tcgcgacgcg tccgaagctc gtgctgctcg acgagccgat ggccggcatg ggaccggagg
     6721 aatccaagcg catgatcgag ctcatcggcc gtatccgcac ggacgtcgcg gtgcttctgg
     6781 tggagcacga catggacgcg gtgttccgcc tcgcggaccg catctcggtg atggtgaacg
     6841 gccgcctcat cgcgacgggt tttcctgaac aaatacgaat gaatgaagaa gtgcgcaagg
     6901 cctacctggg ggacgaactt gctgcttgag gtggacaacc tcgaaaccgc ctatggcacg
     6961 agccaggtgc tgttcggcct ttccttttcg gctgccgaag gcgaagccgt aacgctgctg
     7021 ggccggaacg gaatgggcaa gaccacgacc gttcgctcga tcctcggcct cactcgcgcg
     7081 cgcggcggcg cggtcagctt ccgcgggcgc aagatcgagc gcgagccgcc cgaccgcatc
     7141 gcgcgcatgg ggatcgcgct cgtgcccgag gggcggcaga tcttccccaa tctgagcgtt
     7201 cgcgagcacc tggtcgcctt tgccgaaaac cgttcaggcg ggagcgaccc ctggaccccc
     7261 gaaaaggtgt tccagctttt tccccgcctc ggcgaaaggc agaacaacca gggaaaccag
     7321 ctttccggcg gcgagcagca gatgctcgcc atcggccgcg cgctcgtcac gaacccgcat
     7381 ctcctgatcc tcgacgaagc gaccgaaggc ctcgcgccgc tcgtgcgcga ggagatctgg
     7441 cgctgcctcg ggcggctgcg cgccgccgga cagactatcc tggtgatcga caagtacatc
     7501 gagcgcctgg tgaggctcgc cgatcgccat accatcatcg agcgcgggcg cgtggcctgg
     7561 cagggaagct cggcggagct tgacgccgac cgcgggctct ggcatcgcta cctcgggata
     7621 tgagtactag aaaagcttgg tgaagatacc gccccgggaa atcgcaaaaa gcaggggccc
     7681 gggccggccc gcggtccggc gaaccatgcc gggaaggcag tgcaccagca actcgcctaa
     7741 ttcatttccg ttcatgtcct tgccggtcgc cacgaatccc ctcagctgcg tcgcacggca
     7801 tgtttcccgt tccaggcgat tacgccggat attctggtcc ttggttatca gggcacccag
     7861 ccacgacggg ccacttcagg tatccactcc acgtccagca tgtcgcccgg gaagtgatcg
     7921 agatggacct ccaccttggc accggcggac cgaagcattt cggccacctg atagcggccg
     7981 atctggtgat ctaggaaaaa ggtgagcgag gcaagccgct cggcctcacg ctgcgcgacg
     8041 ctcgcaccgg atggcattct gaattgcctc cggcggtgct gcatagtcgc gcgcgaggac
     8101 atcaatgtcg tcgcccgcgt cgaaccgctc ggcaaggatt gccgttcgca ctccaaactg
     8161 atcgagcacc ggacggccga acgacaatct cgggtcgatc accacatctg cactcttagt
     8221 cttgtcgcgt tgcgcgggga acagcactat cctttgcggg acgcccttag ggtcccgttg
     8281 cactcgcttc agcctgccgc ggatgatttc gcgcatctga atttgacctt cctgggagac
     8341 gccgatcagc gaacccatct tctcgacgaa cagatcgacc ccgtcggttt caaattgctc
     8401 gtctgccagt ggtcgtttgc tttggaaggc ccggcgcagg taatcgactg ccctgcggac
     8461 cttggggagc ggtatgtcgt gttcgcgacg aatgccggaa aggacaaacg cctcaacgag
     8521 gttgatgaac gacaactgtc gttccctgcg gtcggcaatt tcgatcaccg gcttgaaaaa
     8581 cttggcttgt tccccggcac ggtaatgctg tcccatcatc caggctcgaa gggttgacac
     8641 gggcagacga agatagccgg cagcttcggg aatgctgtac gccggatgct ctctaaggtc
     8701 cctgtcagtg aaagcgttt
//
LOCUS       DASTPD010000314         8567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_578428, whole genome shotgun sequence.
ACCESSION   DASTPD010000314 DASTPD010000000
VERSION     DASTPD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578428"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2101)
                     /locus_tag="VFR57_10485"
     CDS             complement(<1..2101)
                     /locus_tag="VFR57_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0259828.1"
                     /translation="MRFDRFTTKLQEALAQAQSLALGQDNQYIEPQHLLGALLEDQGI
                     AGLIAKAGGNAQVLKKNLGDAISRLPKVEGTPGEVHISRELAQLLNVADKEAQKRGDQ
                     YIASELFLLAAAGDKGETGRLLKQSGVTKPTLEKAIREVRGGESVSSQQGEEQRQALA
                     KYTLDLTERARQGKLDPVIGRDDEIRRCIQILQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPESLKNKRVLVLDMAALLAGAKYRGEFEERLKSVLKELAKDEGNTIVFID
                     ELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYLEKDAALERRFQKV
                     LVGEPSVEATIAILRGLQERYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAAARIKMEIDSKPEAMDRLERRLIQLRIEREAVKKETDEASRKRLSLIEDEIKRLE
                     KEYADLEEEWKAEKAQVQGSQHIKEEIEKLRQQMAELQRKGQYDKLAELQYGKLPQLE
                     AQLKKAESSERTKPKLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLIKMEEKLHE
                     RVVGQDEAVRLVSDAIRRSRSGLADPNRPYGSFLFLGPTGVGKTELCKALAAFLFDSE
                     DRLIRIDMSEYGERHSVARLIGAPPGYVGYEEGGQLTEQVRRKPYSVILFDEVEKAHA
                     DVFNVFLQILDEGRLTDG"
     gene            2194..4023
                     /gene="ftsH"
                     /locus_tag="VFR57_10490"
     CDS             2194..4023
                     /gene="ftsH"
                     /locus_tag="VFR57_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0259829.1"
                     /translation="MEKRTQFNLGYLLFALVAIFMLQQWWQQAQTIEVVPYSEFEKLL
                     AADKIAEVVVSDQRITGKLKAPENGKTVAVANLVEPDLAARLSKYGVKYTKIHESTFL
                     RDVLSWVVPALVFFGLWYWLIRRMASQGGGLGGGFMSIGKSRAKVYVEKQTGVTFADV
                     AGVDEAKAELQEVVEFLKDPKKYGRLGARIPKGILLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARQKAPAIIFIDELDALGRARGAYGIGGHDE
                     KEQTLNQLLVELDGFDPAAGVVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKGGRI
                     QILAVHSGKIKLEKDVSLEQVAALTPGFTGADLANLINEAALLATRRGAEAVAMRDFT
                     EAVERLIAGLEKKNRVLNPAERRVVAHHELGHAMVAMALPGTDPVHKVSIIPRGVGAL
                     GYTIQRPTEDRFLMTREELENKMAVLLGGRAAEEVVFGHLSTGAADDLAKVTDIARSM
                     VMRYGMVKNLGYVAYEGERAPFLGGPLPQGHREYSEQTAREIDVAVRDIVKTAHEKAL
                     GILLREKQLLERWATKLLEKETLVEAELAELRAAMLLPRTAAA"
     gene            complement(4038..5981)
                     /locus_tag="VFR57_10495"
     CDS             complement(4038..5981)
                     /locus_tag="VFR57_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEU0259830.1"
                     /translation="MRLPAGRLAPAFLIAALFVVASTVTRALLALRADLAFTGALGEW
                     LRIFACGFSFDVVMATCVTLPIALWLALLPDVAARSRLHRALTVLVFAAFAYVAILLA
                     LAEWLFWDEFGSRFNFIAVDYLLYTHEVLGNIWQSYPVGRLLLALVLGAALLTVPVAK
                     PLWHSAGEPAHWGRSLLAAAVWIAAVASTLRFVDSDMRNVSTRDTANELAGNGLYEFF
                     AANYRNELSYDRHYATYPIEEALALVRRELGAEGEGTERHIRAPRAERRLNVVLVSVE
                     SLGAEFLGSYGNPKGLTPNLDRLAGESLWFSRVYATGNRTVRGLEALALALPPTPGQS
                     IVRRPRNDTLFSLGSVFEDKDYAVLFAYGGYGYFDNMNAFFDSNDYRSLDRRSIPSRR
                     VEHETVWGVADEHLFDQVLDEIDREQQARPQRPFFVHVMTTSNHRPYTYPAGRIDIPS
                     GSGRDGAVKYTDYAIGRFLEQARAKPWFADTVFVITADHGANARGSSRIPIENYLIPV
                     FVYAPCHVAPQRVDRLMSQIDVPPTLLGLLDFDYFSKFFGRDVLNSPPSSDRAFVANY
                     QTLGYLTLERLVLLQPRRKAEVFRVDGDQRVLEPVNDPQALREAIAFYSVASYVFRSG
                     LYRDEEQTPFEQRAALPRVRSGS"
     gene            complement(6042..6497)
                     /locus_tag="VFR57_10500"
     CDS             complement(6042..6497)
                     /locus_tag="VFR57_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0259831.1"
                     /translation="MKISVQTEDFDLSTEVIEISGNPRTGAVASFVGQVREVPMTLEH
                     YPGMTENAIRKIVEEARSQWQILDYAVIHRYGDLQPGDQIVLVAIASSHRGDAFAACQ
                     FIMDYLKTQAPFWKKEHYPDGARWVDARDSDDQAAERWRGLKQQRVLKP"
     gene            complement(6499..6756)
                     /gene="moaD"
                     /locus_tag="VFR57_10505"
     CDS             complement(6499..6756)
                     /gene="moaD"
                     /locus_tag="VFR57_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEU0259832.1"
                     /translation="MKVKVLFFANLRERLGAGAEVVEIPDSASTVAGLRLHLMRRGGA
                     WEEVLGDMKVVRVAVNQDMAAANAPLNPGDEVAFFPPVTGG"
     gene            complement(6753..7946)
                     /gene="glp"
                     /locus_tag="VFR57_10510"
     CDS             complement(6753..7946)
                     /gene="glp"
                     /locus_tag="VFR57_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0259833.1"
                     /translation="MNQGLLSVDDALARLLAEAKPVAEIEDVPTLEATNRILARSQRS
                     TMDVPPMDNSAMDGYAIKAGDAERLRVTQKVMAGSAGKPLEPGTAARIFTGAPIPPGA
                     DAVVMQEHCETQGDWVLVRKKPRPGDWIRLTGSDVKKEAEILPAGKRLLPQDTGLAAS
                     VGIKTLPVYRKVRLGLFFTGDELVMPGEPLAPGRIYNSNRFTLRGLGEVFGCEVHDYG
                     IVPDNLDATREALRRAAAETDILVTSGGVSVGEADFVKPAVEAEGKLLMWRIAMKPGR
                     PLAFGSVGNSFFIGLPGNPVSSFVTFLIFVRPFLLRMQGLSEVEPKAIAARADFDWPE
                     PDARREFLRVKWNAQGGLDLYPTQDSAVLTSTAWADGLVDNPAAHAIRKGDPVRFLPY
                     SELYW"
     gene            complement(7943..8434)
                     /gene="mobB"
                     /locus_tag="VFR57_10515"
     CDS             complement(7943..8434)
                     /gene="mobB"
                     /locus_tag="VFR57_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU0259834.1"
                     /translation="MKVFGFAGWSGSGKTTLIEQLIPRFVKRGLGVSLIKHAHHTFDV
                     DQPGKDSYRHRHAGASEILVTSSRRWVLMHELRGAHEPPFEEQLKRMSPCDVLLVEGF
                     KFAPIPKLEVWRAETGEPLLHPNDPHIVAVASDAKVETKLPLLDLNDVDGIASFILKK
                     LEL"
BASE COUNT         1454 a         2833 c         2776 g         1504 t
ORIGIN      
        1 gtccgtccgt gaggcggcct tcgtccagga tctgcaggaa cacgttgaac acgtccgcgt
       61 gcgccttctc gacctcgtcg aagaggatca ctgagtaggg cttgcgccgc acctgctcgg
      121 tgagctgccc gccctcctcg tagcccacgt atcccggcgg cgcgccgatc aaccgcgcga
      181 cggaatggcg ctcgccgtac tcggacatgt cgatgcggat caggcgatcc tcggaatcga
      241 acagaaacgc ggcgagcgcc ttgcaaagct cggtcttgcc aactcccgtc ggcccgagga
      301 aaaggaatga cccgtaaggc cggttgggat cggcgagccc cgagcgcgag cgccggatcg
      361 cgtcggacac gaggcgcacc gcctcgtcct gcccaacgac gcgctcgtgc agcttctctt
      421 ccatcttgat gagcttgtca cgctcgccct gcatcatctt cgacaccggg atgccggtgg
      481 cgcgcgacac gacctcggcg atctcctcgg cgccgacctg cgtgcgcagc agcttgggct
      541 ttgtcctctc gctggactcg gccttcttta gctgggcttc gagctgaggc agctttccat
      601 actgcagctc ggcgagcttg tcgtactgcc ccttcctctg cagctcggcc atctgctggc
      661 gtagcttctc gatctcttcc ttgatgtgct ggctgccctg gacctgcgcc ttctcggcct
      721 tccactcttc ctcgagatcc gcgtactcct tctccagccg cttgatttca tcctcaatga
      781 gagaaagtct cttgcgcgac gcctcgtcgg tctctttctt gacggcctcg cgttcgatcc
      841 gaagctggat caggcgccgc tcgaggcggt ccatcgcctc gggcttggag tcgatctcca
      901 tcttgatgcg cgcggccgcc tcgtcgatca gatcgatcgc cttgtcgggc aggaagcggt
      961 cggtgatgta gcggtgcgaa agctcggccg cggcaacgat cgccgggtcg gtgatctcga
     1021 cgccgtggtg cagctcgtag cgctcctgca ggccgcgcag gatggcgata gtcgcctcca
     1081 cgctcggctc gccaaccagc accttctgga agcggcgctc gagcgccgcg tccttctcga
     1141 ggtacttgcg gtactcgtcg agcgtcgtcg cgccgatgca atgcagctcg ccgcgggcaa
     1201 gcgcgggctt gagcatgttg ccggcgtcga tcgcgccctc ggccttgccg gcgccgacca
     1261 tggtgtgcaa ctcgtcgatg aagacgatgg tgttgccctc gtccttcgcc agctctttca
     1321 gcaccgactt cagccgctcc tcgaactcgc cgcggtactt cgcgcccgcg aggagggctg
     1381 ccatgtcgag taccagcacg cgcttgttct tgaggctctc gggaacttcg ccgttgacga
     1441 tgcgctgcgc gagcccttcc acgatcgcgg tcttgccgac ccccggctcg ccgatcagta
     1501 cggggttgtt cttcgtgcgc cgctgcagga tctggatgca gcggcggatc tcgtcgtcgc
     1561 gcccgatgac cgggtcgagc tttccttgcc ttgctctttc agtcagatcc aatgtgtact
     1621 tggcgagcgc ctgcctttgc tcctctccct gctgggaaga aacgctctcg ccgccccgca
     1681 cttcccggat cgctttctcc agcgtgggct tcgttacgcc ggattgcttg aggagccttc
     1741 cggtctcgcc cttgtcaccg gcggcggcga gcagaaagag ctcggaggcg atgtattggt
     1801 cgccgcgctt ctgtgcctcc ttgtcggcga cgttgaggag ctgcgcgagc tcgcgggaga
     1861 tgtgaacttc cccgggcgtg ccttccacct tgggaagcct ggaaatcgca tcgcccaggt
     1921 tcttcttcag gacctgcgca ttgccgccgg ccttggcgat caggccggcg atgccctggt
     1981 cctcgagcag cgccccgagc aggtgctgcg gctcgatgta ctggttgtcc tggccaagcg
     2041 caaggctctg cgcctgcgcc agggcctcct gcagcttggt ggtgaatctg tcgaatctca
     2101 tagatactcc cttacgccaa atggggatcc gcagggccct ttcaaggctt attcttgctt
     2161 ggaaagtggg ggctttaccc caatctcgac ctgatggaaa agcgcaccca gttcaacctc
     2221 ggctacctgc tgttcgccct ggtcgccatc ttcatgctcc agcagtggtg gcagcaggcg
     2281 cagacgatcg aggtcgtgcc ctacagcgag ttcgaaaagc tgctcgcagc ggacaagatc
     2341 gccgaggtcg tggtgtccga ccagcgcatc accggcaagc tgaaagcgcc cgagaacggc
     2401 aagaccgtgg cggtggccaa cctcgtcgag ccggacctcg ctgcgcggct ttccaagtac
     2461 ggggtcaagt acacgaagat ccatgaaagc accttcctgc gcgacgtgct gtcgtgggtg
     2521 gtccccgcgc tcgtcttctt cggcctctgg tactggctca tccgccgtat ggcgagccag
     2581 ggcggcgggc tcggcggcgg cttcatgtcg attggcaaga gccgcgccaa ggtctacgtc
     2641 gagaagcaga ccggcgtcac cttcgccgac gtggccgggg tcgacgaagc gaaggccgag
     2701 ctgcaggagg tggtggagtt cctcaaggac ccgaagaaat acggccgcct cggcgcgcgc
     2761 atcccgaagg gcattctgct cgtcggcccg cccggcaccg gcaagacgct gctcgcgcgc
     2821 gccgtagccg gcgaagccgg cgtgcccttc ttctcgatct ccggctcgga gttcgtcgag
     2881 atgttcgtcg gcgttggcgc cgcgcgcgtc agggatttgt tcgagcaggc gcggcagaag
     2941 gcgcccgcga tcatatttat cgacgagctc gacgcgctcg ggcgcgcgcg cggcgcctac
     3001 ggcatcggcg gccacgacga gaaagagcag accctgaacc agctcctcgt tgagcttgac
     3061 ggcttcgatc cggcggcggg cgtagtgctg ctcgcggcga ccaaccgccc ggaaattctc
     3121 gaccccgcgc tgctgcgcgc cgggcgcttc gaccggcagg tgctggtcga ccggccggac
     3181 aaaggcggac gcatccagat ccttgccgtt cattccggga agataaagct cgaaaaggac
     3241 gtaagcctgg agcaagtggc tgcgctaacc ccgggcttca ccggcgccga cctcgccaat
     3301 ctgatcaacg aagcggcgct cctcgccacg cggcgcggtg ccgaggcggt ggcgatgcgc
     3361 gacttcaccg aggcggtgga gcggctcatc gccgggctgg agaagaaaaa ccgcgtgctc
     3421 aacccggcgg agcgccgcgt ggtcgcccac catgagctcg gccacgccat ggtggcaatg
     3481 gcgcttcccg ggacggaccc cgtgcacaag gtatcgatca tcccgcgcgg ggtcggcgcg
     3541 cttggctaca ccatccagcg tccgaccgag gatcgcttcc tgatgacgcg agaggagctc
     3601 gagaacaaaa tggccgtgct actcggcggc cgtgctgccg aggaggtcgt gttcggccat
     3661 ctctcgaccg gcgcggccga cgatctcgcg aaagtgaccg atatcgcccg cagcatggtg
     3721 atgcgctacg gcatggtgaa gaacctcggc tacgtcgcgt acgaaggaga gcgcgcgccg
     3781 ttcctcggcg ggccgctgcc gcagggccat cgtgaataca gcgaacaaac cgcgcgcgag
     3841 atcgacgtcg cggtgcgcga catcgtgaag acggcccatg agaaggcgct cggtatccta
     3901 ttgcgtgaga agcaactgct cgagcgctgg gcgacgaagc tgctcgagaa agaaactctg
     3961 gtcgaggccg agctcgccga gctgagggcc gcgatgttac tgccgcgcac ggcggcagcg
     4021 taaccgctcg agctaaccta gctgccgcta cgtacccgtg gcagcgcggc gcgctgttcg
     4081 aaaggcgtct gctcctcgtc ccgatagagg ccgctgcgga atacgtagga ggcgaccgaa
     4141 tagaacgcga tcgcttcgcg cagcgcctgc gggtcgttca ccggctcgag aacgcgctgg
     4201 tcaccgtcga cccggaatac ctcggccttg cgacgcggct ggagcagcac caagcgctcg
     4261 agcgtcagat agccgagcgt ttggtaattg gcgacaaacg cgcgatcaga gctcggcggc
     4321 gaattgagca cgtcgcggcc gaagaacttg ctgaagtaat cgaagtccag caggccgagc
     4381 agcgtcggcg gcacgtcgat ttgcgacatc aggcggtcca cgcgctgcgg cgcgacgtgg
     4441 cacggcgcgt agacgaacac ggggatgagg tagttctcga tcggaatccg gctcgagcca
     4501 cgcgcgttcg cgccgtggtc agctgtaatg acgaacaccg tgtctgcgaa ccacggcttg
     4561 gcgcgcgcct gctcgaggaa cctgccgatc gcgtagtcgg tgtacttgac cgcgccatcg
     4621 cgcccgctcc ccgagggaat atcgatgcgc ccggcgggat aggtgtacgg gcggtggttg
     4681 ctcgtggtca tgacatgaac gaagaacggc cgctgcggcc gcgcctgctg ctcgcggtcg
     4741 atctcgtcga gcacctggtc gaacaggtgc tcgtcggcca cgccccagac ggtctcgtgt
     4801 tcgacgcgcc gcgacggtat cgaacgccgg tctaaagagc ggtagtcatt gctatcgaag
     4861 aacgcgttca tgttgtcgaa gtaaccgtag ccgccgtagg cgaagagcac cgcgtagtcc
     4921 ttgtcctcga acacgctgcc cagggagaac agcgtgtcgt tgcgcgggcg gcgcacgata
     4981 gactggccgg gcgtcggcgg aagggccagc gcaagcgctt ccagcccgcg cacggtgcga
     5041 ttgccggtcg catagacgcg cgagaaccac aggctttcgc cggcgaggcg gtccaggttg
     5101 ggggtgagcc ccttggggtt accgtagctg ccgaggaatt cggcgcccag gctctccacg
     5161 ctcaccagca cgacgttcag cctgcgctcg gcgcgcggcg cccggatatg gcgctcggtg
     5221 ccctcgccct cggcgcccag ctcgcggcgc accagagcca gcgcctcctc gatagggtag
     5281 gtcgcgtaat ggcggtcgta gctaagctcg ttgcggtaat tagccgcgaa gaactcgtac
     5341 aagccgttgc cggcgagttc gttggccgtg tcgcgcgtcg agacgttcct catgtcgctg
     5401 tcgacgaagc gcagcgtgct ggccaccgcg gcgatccaga ccgccgctgc gaggaggctc
     5461 cggccccagt gcgcgggctc gccggcggag tgccatagcg gcttcgccac cggaaccgtg
     5521 agcagtgccg cgccgaggac cagcgcgagg agcaggcggc ccacgggata gctttgccag
     5581 atattgccga gcacctcgtg cgtgtaaagc aggtagtcga ccgcgatgaa attgaagcgc
     5641 gagccgaact cgtcccagaa caaccactcc gccaaggcga gcaggatcgc cacgtaggca
     5701 aacgcggcga acacaagaac cgtgagcgcg cggtgaagcc ggctgcgcgc cgcgacatcc
     5761 ggcagcagcg cgagccagag cgcgatcggc agcgtgacgc aggtcgccat cacgacgtcg
     5821 aaagaaaacc cgcaggcgaa aatgcgcagc cactcgccga gcgcgcccgt gaaagcaagg
     5881 tcggcgcgca gagcgagcag cgcgcgcgtg acggtggagg ctacgacgaa cagcgctgcg
     5941 atcaggaatg ccggcgccag tcggccggcg gggagccgca tttgggcgcg taacgtacgc
     6001 gccgcacgtt aaccgaagat taagcccgcc gagcgttcaa ctcacggctt gagtacgcgc
     6061 tgctgcttga ggccgcgcca gcgctcggca gcctggtcgt ccgagtcgcg tgcgtcgacc
     6121 cagcgcgcac cgtcggggta gtgctccttc ttccagaacg gcgcctgagt cttcaggtaa
     6181 tccatgatga actggcaggc ggcgaacgca tcgccccggt gcgagctcgc gatcgcgacc
     6241 aggacgatct ggtcgcccgg ctggagatcg ccgtagcggt ggatcacggc gtaatcgagg
     6301 atctgccact gcgagcgcgc ctcctcgacg atcttgcgga tggcgttctc ggtcatgccc
     6361 gggtagtgct cgagcgtcat cggcacctcg cgcacttgcc cgacgaagct cgctaccgcg
     6421 cccgtcctcg ggtttccgga gatctcgatc acctcggtgc tgagatcgaa gtcttcggtc
     6481 tgcaccgaga ttttcacgtc agccgccggt cacgggcgga aagaaggcga cttcgtcgcc
     6541 cgggttgagc ggcgcgttgg ccgcggccat gtcctggttg accgcgacgc gtaccacctt
     6601 catgtcgcca agcacctcct cccacgcgcc gccgcgccgc atgagatgca gccgcaggcc
     6661 cgccacagtg ctcgccgaat ccgggatctc gacgacctcc gcccccgcgc caagccgctc
     6721 gcgcaggttg gcgaaaaaca gcactttcac tttcaccagt acagctccga atagggcagg
     6781 aagcgcaccg gatcgccttt gcgaatcgca tgcgcggcgg gattgtccac caggccgtct
     6841 gcccaggcgg tcgaagtaag gaccgccgaa tcctgagtcg gatagagatc cagcccgccc
     6901 tgcgcgttcc attttacgcg caggaactcg cgccgcgcgt cgggctcggg ccagtcgaaa
     6961 tccgcgcgcg cggcgatggc tttcggttcc acctctgata atccctgcat acgcagcagg
     7021 aacggcctga cgaagatcag gaacgtgacg aagctggaaa cggggttgcc cggcagcccg
     7081 atgaagaatg aattcccgac cgagccaaaa gcgagtggac gacccggctt catcgcgata
     7141 cgccacatca agagctttcc ttccgcctcg accgcgggct ttacgaaatc cgcctcaccg
     7201 accgacacgc cgcccgaggt gacgaggatg tccgtttccg ccgcggcgcg gcgcagcgct
     7261 tcgcgcgtcg catcgaggtt gtccggaacg atgccgtagt cgtgcacttc gcagccgaac
     7321 acctcgccca gaccgcgcaa ggtgaagcgg ttcgagttgt agatccgacc gggagcgagc
     7381 ggctcgcccg gcataacgag ctcgtcgccg gtgaagaaca accccaatcg caccttccgg
     7441 tacaccggca gcgtcttgat gcccaccgag gccgcgaggc cggtgtcctg cggcagcagg
     7501 cgcttcccgg cgggcaagat ttcagcctcc tttttcacgt cgctcccggt gaggcggatc
     7561 caatcgcccg gtcttggctt tttcctcacc aagacccaat caccttgggt ttcgcaatgc
     7621 tcctgcatca ccacggcgtc cgcgcccggc ggaatcggcg cgccggtaaa gatgcgcgcg
     7681 gcggttcccg gttccagcgg ctttcctgcc gaacccgcca tgactttttg tgttacgcgc
     7741 aggcgctccg catccccggc cttgattgca tacccgtcca tggcgctgtt gtccatcggc
     7801 ggcacgtcca tggtggagcg ctgcgagcgt gcgaggatgc ggttggtggc ttcgagcgtc
     7861 ggcacatctt cgatttccgc caccggcttc gcttccgcca gcaggcgcgc gagcgcgtcg
     7921 tcgaccgaga gaagtccctg gttcacaact cgagcttttt caggatgaag ctagcgatgc
     7981 cgtccacatc gttgagatcg agcaatggca gcttggtttc caccttcgca tcgctcgcca
     8041 cagccacgat gtgcggatcg ttggggtgca ggagcggctc gccggtctcg gcgcgccaca
     8101 cttcgagctt gggaatcggc gcgaacttga agccctcgac cagcagcaca tcgcacggcg
     8161 acatgcgctt cagctgctct tcgaacggcg gctcgtgcgc gccgcgcagc tcgtgcatca
     8221 gcacccagcg ccgcgacgag gtgacgagga tttccgaggc gccagcatgg cggtgacggt
     8281 acgagtcctt gccgggctga tcgacgtcga acgtatggtg cgcgtgcttg atgagcgaca
     8341 cgcccagccc gcgcttcacg aagcggggaa taagctgctc gatgagcgtg gtcttgccgc
     8401 ttccagacca gccggcgaag ccaaacacct tcatacccag atcattttcg caccggcgag
     8461 ggcaaggact acggcgagca cgcgccgcac gaccgggttt gcgaaacgcc ggctgccgta
     8521 ttcggcgccg agccagccgc cgatgattac cgccggagcg agaatcc
//
LOCUS       DASTPD010000315         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1193576, whole genome shotgun sequence.
ACCESSION   DASTPD010000315 DASTPD010000000
VERSION     DASTPD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193576"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2496
                     /gene="gyrA"
                     /locus_tag="VFR57_10520"
     CDS             <1..2496
                     /gene="gyrA"
                     /locus_tag="VFR57_10520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0259835.1"
                     /translation="GLKPVHRRVLYAMHEANNVWNRPYVKCARIVGDVLGKYHPHGDS
                     ATYEALVRMAQDFSMRYTLIDGQGNFGSVDGDAAAAYRYTECRLEKISAELLADIGAE
                     TVDFTPNYDGKELEPAVLPTRAPNLLVNGSSGIAVGMATNIPPHNLGEVVDACLHLLV
                     QPHCAIEELIKLMPAPDFPTSGIIYGLAGVHEGYRTGRGRAVMRARTHFEDIGKGERQ
                     AIIVDELPYQVNKRVLLEKIAEMVNEKRLEGISDIRDESDKSGMRAVLELKRGERPEV
                     VLNNLYKTTQLQDSFHMNMVALVDGQPRLLNLKDLLAAFIAHRREVVTRRTVYELRQA
                     RARGHVLEGLAVALSNVDPVIELIKKAPSPAEAKRGLMERAWGSELVRSLVGNATAAR
                     FRPEGLAQEFGLQEEGYRLSEEQAQAILELRLQRLTALEQDKIRAEYREVIVAITDLL
                     DILAKPARITKIIDEELRKLKAEFADKRKSEIVTVAEDISIEDLIAPQDMVVTFSHGG
                     YVKSQPLADYRAQRRGGRGRMATSMKEDDFIERLFVAHSHDYLLCFSDRGRLYWLKVY
                     EVPVGSRASRGKPIVNMFPLEEGEKITAVVPVKEFDENHYVFMATAHGTVKKTPLAEF
                     SRPRPSGIIAVGLDQGDYLVGCVLTDGGYDVMLFSSEGKSVRFPESDVRPMGRQATGV
                     RGMRLDKGQRVVCMLAAKDDSRSVLTATANGYGKRTAIADYPRHGRGGQGVIAIQVSE
                     RNGALIGAVLVDEHDEVMLISTGGVLIRTAVSQIREMGRSTQGVTLISLDAGEKLAGM
                     ERIEERDLGNGNGGNGENHEEVPPPETPPPTVH"
     gene            2496..>3437
                     /gene="serC"
                     /locus_tag="VFR57_10525"
     CDS             2496..>3437
                     /gene="serC"
                     /locus_tag="VFR57_10525"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEU0259836.1"
                     /translation="MSRIFNFSAGPAMLPAEVLARAGDEMLDWRASGMSVMEMSHRGP
                     QFMSIAAEAEADLRALLAIPANYKVLFLQGGATLQFAQVPMNLLRGKGKADYVLTGEW
                     SKKAVKEAKNYCDVAIAASSEDRNFTYAPKQFSVRKEAAYVHYCSNETIGGVEYHSVP
                     YQGEIPLLADASSHFLSRPMDVSKYGLIYAGAQKNAGPAGLTIVIVRDDLIGQAAKGT
                     PSVMDYKRQADAESMLNTPATYSLYVAGLVFKWLKQQGGLASIERKNVEKAKLLYDYL
                     DSSEFFHNPVAKEDRSRMNVPFTLKDKSLDEPFLKGAA"
BASE COUNT          663 a         1104 c         1134 g          536 t
ORIGIN      
        1 ggcctgaagc ccgtccaccg gcgcgtgctg tatgcgatgc acgaggcgaa caacgtctgg
       61 aaccggccct acgtgaagtg cgcgcggatc gtcggcgacg tgctcggcaa gtaccacccg
      121 cacggcgatt cggccaccta cgaagcgctg gtgcgcatgg cgcaggactt ctcgatgcgc
      181 tacacgctga tcgacgggca gggcaatttc ggctcggtcg acggcgatgc ggccgcggcc
      241 taccgctata ccgagtgccg gctcgaaaaa atttcggccg agctgctcgc cgacatcggg
      301 gccgaaaccg tcgacttcac accgaactac gacggcaagg agctcgagcc cgcggtgctg
      361 ccgacgcgcg cgcccaacct tctggtgaac gggtcgtcgg gtatcgcggt cggcatggcg
      421 accaacatcc cgccgcacaa cctgggcgaa gtggtcgacg cctgcctgca cctgctcgtg
      481 cagccgcact gcgcgatcga ggaactgatc aagctcatgc cggcgcccga tttcccgacc
      541 tcggggatca tctacggcct cgccggcgtg cacgaaggct accgcaccgg ccgcgggcgc
      601 gcggtgatgc gcgcgcgcac ccacttcgag gacatcggca agggcgagcg ccaggcgatc
      661 atcgtcgacg agctgcccta ccaggtgaac aagcgcgtgc tgctcgagaa gattgccgag
      721 atggtgaacg aaaagcgcct cgagggaatc tcggacatcc gcgacgagtc cgataaatcg
      781 ggcatgcgcg cggtcctgga gctcaagcgc ggcgagcggc ccgaggtggt gctcaacaac
      841 ctctacaaga cgacccagct gcaggacagc tttcacatga acatggtggc gctggtcgac
      901 ggccagcccc gcctgctcaa tctcaaggat ctgctcgcgg ccttcatcgc gcaccgccgc
      961 gaggtcgtta cgcgacgcac ggtgtacgag ctgcgccagg cgcgcgcgcg gggccacgtg
     1021 ctggaggggc tcgcggtcgc gctgtccaac gtcgacccgg tgatcgagct gatcaagaaa
     1081 gcgccgagcc cggccgaggc caagcgcggg ctgatggagc gcgcctgggg ttcggagctg
     1141 gtgagaagcc tggtcggcaa cgccaccgcg gcgcggtttc ggccagaagg gcttgcacag
     1201 gagtttggct tgcaggaaga aggctatcga ctctcggagg agcaggcgca agcgatcctc
     1261 gagctacggc tgcagcgcct gaccgcgctc gagcaggaca agattcgcgc cgagtaccgc
     1321 gaggtgatcg tcgcgatcac cgatctgctc gacatcctgg cgaagcccgc gcgcatcacg
     1381 aagatcatcg acgaagagct gcgcaagctg aaggccgagt tcgccgacaa gcgcaagagc
     1441 gagatcgtca ccgttgccga ggacatctcg atcgaggacc tgatcgcgcc gcaggacatg
     1501 gtggtcactt tctcgcacgg cggctacgtg aagtcgcagc cgctcgccga ctaccgggcg
     1561 cagcgccgcg gcggacgggg ccgcatggcg acttcgatga aggaagacga cttcatcgag
     1621 cgcctgttcg tcgcgcactc gcacgattac ctgctgtgct tctccgatcg cgggcgactc
     1681 tactggctca aggtctacga agtcccggtg ggcagccgcg caagccgcgg caagccgatc
     1741 gtcaacatgt tcccgctgga ggagggcgag aagatcaccg cggtcgtgcc ggtgaaggag
     1801 ttcgacgaga accactacgt gttcatggcg accgcgcacg gcacggtgaa gaagactccg
     1861 ctcgcggagt tttcgcgccc gcggccgagc ggcatcatcg cggtcggtct ggaccaaggt
     1921 gactacctgg tcggctgcgt cctcaccgac ggcggctacg acgtgatgct gttctcctcc
     1981 gagggcaagt cagtccgctt cccggaaagc gacgtgcggc cgatgggccg ccaggcgacc
     2041 ggcgtgcgcg gcatgcgcct cgacaaaggc cagcgcgtgg tatgcatgct cgccgccaag
     2101 gacgattccc gctcggtgct caccgccacc gcgaacggct acggcaagcg caccgcaatt
     2161 gccgactacc cgcgccacgg gcgcggcggc cagggcgtga tcgcgatcca ggtgtccgag
     2221 cgcaacggcg cgctcatcgg cgcggtgctg gtggacgagc acgacgaggt catgctcatc
     2281 tcgaccggcg gcgtgctcat ccgcaccgcg gtgtcccaga ttcgcgaaat gggccgctcg
     2341 acccagggcg tcaccctgat ctcgctcgac gcgggcgaga agctcgccgg catggagcgc
     2401 atcgaggagc gcgacctcgg caacggcaac ggcggtaatg gcgaaaatca cgaggaagtt
     2461 ccgccgcccg agacgcctcc ccccaccgtt cactgatgtc ccgcatcttt aatttcagcg
     2521 cgggacccgc gatgcttccc gcagaagtcc tcgctcgcgc cggcgacgag atgctcgact
     2581 ggcgcgcgag cggcatgtcg gtgatggaaa tgagccaccg cggcccgcaa ttcatgtcga
     2641 tcgccgccga ggccgaagcc gacctgcgcg cgctgctcgc catcccggcg aactacaagg
     2701 tgctcttcct gcagggcggc gcgacgctcc agttcgcgca ggtgcccatg aatctgctgc
     2761 gaggcaaggg caaggccgac tacgtgctga ccggcgaatg gtcgaaaaag gcggtaaagg
     2821 aagcgaagaa ctactgcgac gtggcgatcg ctgcgagctc cgaggaccga aatttcacgt
     2881 atgcgccgaa acaattttcg gtaagaaaag aggccgccta cgttcactac tgctcgaacg
     2941 agaccatcgg gggagtcgaa tatcactctg tgccttacca gggagaaatc ccgctgctcg
     3001 ccgatgcatc ctcgcacttc ctgtcgcgcc ctatggacgt ttcgaagtac ggcctgatct
     3061 acgccggggc gcagaagaat gccggccctg ccgggctcac gatcgtgatc gtgcgcgacg
     3121 atttgatcgg acaggcggcg aagggcacgc cgagcgtcat ggactacaag cggcaagccg
     3181 acgccgagtc catgcttaat accccggcaa cttattcgct ctatgtcgcc gggctggttt
     3241 tcaagtggct gaagcagcag ggcggtttgg cgtccataga aagaaagaac gttgagaagg
     3301 cgaagctgct gtacgactac ctcgattcga gcgagttctt ccataacccg gtggcgaagg
     3361 aagaccgctc gcgcatgaac gtgccgttca ctttgaagga caaatcgctg gacgagccgt
     3421 tcctgaaggg tgcggcc
//
LOCUS       DASTPD010000316         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1397484, whole genome shotgun sequence.
ACCESSION   DASTPD010000316 DASTPD010000000
VERSION     DASTPD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397484"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFR57_10530"
     CDS             <1..429
                     /locus_tag="VFR57_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0259837.1"
                     /translation="APVWVKHVPRKEAEDTAMHFLERVRIPEQAGKYPSQLSGGQQQR
                     VAIARALCMRPKLMLFDEPTSALDPEMIKEVLDVMVELAREGMTMMVVTHEMGFARTV
                     ADRVVFMDGGRIVEAAAPAEFFGAPKTDRAKLFLSQVLGH"
     gene            complement(443..988)
                     /locus_tag="VFR57_10535"
     CDS             complement(443..988)
                     /locus_tag="VFR57_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0259838.1"
                     /translation="MAKLLVALAALLAASPAPADEKLWQLLKGGGQVVLIRHTITTPG
                     AGDPPGMRLDDCSTQRNLTDAGRAHARRIGEAFRARGIPVERVVSSPWCRCVETAKLA
                     FGQAEISDALSNLFGRPENRERQVKEMRALIAARQGQGNLVLVTHGSTIVALTGVSPD
                     TGEMVLFSQGAAAGRLSVPPG"
     gene            complement(992..1435)
                     /locus_tag="VFR57_10540"
     CDS             complement(992..1435)
                     /locus_tag="VFR57_10540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU0259839.1"
                     /translation="MKILVLHGPNLNLLGRREPHIYGSTTLADINRKLEQLGKELGVE
                     LEIVQSNHEGALVDFLHKHIDTAAGAILNPAGLTQFGVSLHDAIKTMPFPVIEVHISN
                     RHAREEWRHHSIISSAAKATVEGLGWRSYIEALRAVVEMARESRK"
     gene            complement(1451..2578)
                     /locus_tag="VFR57_10545"
     CDS             complement(1451..2578)
                     /locus_tag="VFR57_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0259840.1"
                     /translation="MQALRLYDELTAARTRYLRVDELCRLAAERGYLPSEAELAIEAR
                     LPLKEKKGLEREQGRFLAEILGNPVAGTHLCHAMLLPRADSRTRLMEYEQRGELRLQG
                     ATLKREGKASVVTLSNPHYLNAEDESTLPGAEIAVDVALLDPKTEVCVLRGDVVTHAK
                     YAGRRLFGAGINLTHLYEGKIRYLWYLIRDLGFVNKLYRGLAQENQSPELASTEKLWI
                     AAVEGFAIGGHCQILLTMDYTLAAQDAYLTLPARKEGIIPGAANLRLPRFVGDRIARQ
                     AILAERRIECSSPEGRMICDDIVPSSRMDGAIASMVEKLTSSGVVSAAGNRRAFRAGE
                     EPLDTFRRYMAIYAREQAGCHFSPALIANLERNWKAAKSSG"
BASE COUNT          423 a          849 c          871 g          474 t
ORIGIN      
        1 gccccggtgt gggtgaagca tgtgccgcgc aaggaagccg aggacaccgc gatgcacttc
       61 ctcgagcgtg tgcgcattcc cgagcaggcg ggcaagtacc cgtcgcaact ttccggtggc
      121 cagcagcagc gcgtcgcaat agcgcgcgcg ctgtgcatgc ggccgaagct gatgctgttt
      181 gacgaaccta cttccgcgct cgatccggag atgatcaagg aagtgcttga cgtcatggtc
      241 gaactcgcgc gcgaaggcat gaccatgatg gtggtgacgc acgagatggg cttcgcccgg
      301 accgtggccg accgcgtggt gttcatggac ggcggtcgca tcgtggaggc cgcggcgccg
      361 gccgagttct tcggcgcgcc gaagaccgac cgggcgaagc ttttcttgag ccaggtgctc
      421 gggcactaag cgcctggcgc gctcagcctg gcggaacgga cagcctgcca gcggccgcgc
      481 cctgcgagaa aaggaccatt tctccggtat cgggcgacac cccggtgagc gcgacgatcg
      541 tcgatccgtg cgtaacgagc acgaggtttc cctgaccctg cctggccgcg atgagggccc
      601 gcatctcttt cacctgacgc tcacggttct ccggccgtcc aaagaggttg ctgagcgcgt
      661 ccgaaatctc cgcctggccg aatgcaagct tcgccgtctc cacgcaacgg caccacgggc
      721 tcgacacgac ccgctcgacc ggaatgccgc gcgctcggaa cgcttcgccg atacgccggg
      781 cgtgcgcccg gccggcgtcg gtgaggttgc gctgcgtgct gcaatcgtcg agccgcatgc
      841 cgggcgggtc gcctgcgccg ggggtggtaa tggtgtgccg aatcaggacg acctggccgc
      901 cgcctttcag cagctgccat agcttttcgt cggccggcgc cggggatgct gcgagcaggg
      961 cggcaagcgc gaccagcagc ttcgccacga gctacttacg tgactcccgc gccatttcga
     1021 ccacggcgcg cagcgcctcg atatacgagc gccagcccag accctcgacc gtcgccttgg
     1081 ccgccgatga gatgatcgaa tggtggcgcc attcctcgcg cgcgtgccgg ttggagatgt
     1141 gcacctcgat gaccgggaac ggcatggtct tgatcgcgtc gtgcagggaa acgccgaatt
     1201 gcgtgaggcc ggcgggattg agaatcgcgc ccgcggcggt atcgatgtgc ttgtgcagaa
     1261 aatcgaccag agcgccttca tggttcgact gcactatttc cagctcgaca cccaactcct
     1321 tccccaactg ctccagtttc ctgttgatgt cggcgagcgt cgtactgcca tagatgtgcg
     1381 gctcgcgccg gccgagcagg ttgaggttgg gcccgtgcag cacgaggatc ttcatcggtt
     1441 tttcctgagt ttagccgctg cttttggcgg ccttccaatt tctttcaaga ttcgcaatta
     1501 gggccgggct gaaatggcag ccggcctgct cgcgcgcgta gattgccata taccggcgga
     1561 acgtatcgag cggctcttcg ccggcgcgga acgcacgccg gtttcctgcg gcgctgacca
     1621 cgcccgagga ggtcagcttt tccaccatcg aagctatcgc gccgtccatc ctgctcgaag
     1681 gcacgatgtc gtcgcagatc atccggccct cggggctcga gcactcgatg cgccgctcgg
     1741 ccaggatcgc ctggcgcgcg atgcggtcgc cgacgaagcg cggcaggcgc aggttggcgg
     1801 cgcccgggat gatgccttcc ttgcgcgccg gcaaggtcag gtacgcgtcc tgcgcggcga
     1861 gggtgtagtc catcgtgagc aggatctggc agtggccgcc gatagcgaag ccctccaccg
     1921 ccgcaatcca gagcttttca gtggacgcga gctcaggcga ctggttttcc tgcgccaaac
     1981 ctcggtaaag cttgttcacg aagcccaggt cgcgaatcag gtaccagagg tagcggatct
     2041 tgccttcgta gaggtgcgtg aggttgatac cggcgccgaa gagccggcgc ccggcgtact
     2101 tcgcgtgcgt aacgacatcg ccgcgcagca cgcacacttc ggtcttcggg tcgagcaggg
     2161 cgacgtcgac cgcgatctcg gccccgggca gcgtcgattc gtcctctgcg ttcaggtagt
     2221 gcgggttgct gagcgtcacg accgaggcct tgccctcgcg cttcaacgtc gcgccttgga
     2281 gccgaagctc gccgcgctgc tcgtattcca ttaatcgtgt gcgcgaatct gcacgcggca
     2341 ggagcatcgc gtgacacagg tgcgtgcccg cgaccgggtt gccgaggatt tcggcgagga
     2401 agcggccttg ctcgcgctcg agccccttct tctccttcag cggcagccgc gcctcaatcg
     2461 caagctcggc ctctgacgga agatagcctc tttcggcagc gaggcggcaa agctcgtcga
     2521 cgcgcaagta gcgcgtgcgc gcggcggtga gctcgtcgta gaggcgaagc gcttgcattc
     2581 aggctgcggc cccgcgcagc ggcaggctcc gccgcga
//
LOCUS       DASTPD010000317         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_376288, whole genome shotgun sequence.
ACCESSION   DASTPD010000317 DASTPD010000000
VERSION     DASTPD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376288"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VFR57_10550"
     CDS             complement(<1..1916)
                     /locus_tag="VFR57_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259841.1"
                     /translation="MSATDRVAIVVAHGIADQRPRQTVREVARLLCRGGPDRASYVQG
                     EMQDVLVPVEPLPPGDRVLQDASPRQRPGTPSGFYQAQQSAEVKAQEPKQRAPQTQDL
                     GMALNDYLLSRFVLPESDALYESTRVRLRRRADGRPLDVYEMYWADLSRLGTGGLRAL
                     SSLYQLFFHLSTLAADVVDQVSLSTNGGRAWRLLQRLHAWMAWIMKGPAALLQLAMLL
                     LIVFGAAALVHPDLRGQLVAAIFGLGGVVLAALAVLAWLRAAPGFARWAKLAFLFAVA
                     SFSLAFAVLALAAAGVAPVVYFVASALGIGLLGGYLIERYSGVTRGVRLLGHLIVVAT
                     VLMVWVAGQLVRPTTEIEWMLLSALSAGEVLYGGVLIVWAVFVAVQISALTLGLWLGR
                     GCDKAARASLHTARLALIGSTGLFAVLSLVLWSVVSYIVGRALSDFLYLPHFFGRGYR
                     SAAFFLDDRVQALGGFFTPLVAGFALAFAAALLVVLPSLIEEMKPTANFDDSGAIKGA
                     AEWARRLGNWLTGGIRALGRAFAALIPLAAIAGSALYLAFVLQEFGDAMKIGGDLAWL
                     VHLLDMFQGETLVATGKWLAGGALTIAALGSRFTETFGRLSVAIDAVLDIDNYFADPP
                     NRQPPRARIYSRYAS"
BASE COUNT          424 a          701 c          657 g          287 t
ORIGIN      
        1 gaggcatagc gcgaatagat gcgggcccgc ggcggctgcc ggttgggcgg atcggcgaag
       61 taattgtcga tgtcgagcac cgcgtcgatc gcgacgctta ggcgcccgaa ggtctcggta
      121 aagcgcgagc cgagcgcggc gattgtcagt gcgcctccgg cgagccactt gcccgtggca
      181 accagggtct cgccctgaaa catgtcgagc aggtgcacga gccacgcgag atctcctcca
      241 atcttcatgg cgtcgccgaa ttcttggagg acgaacgcga ggtagagcgc gctgccggca
      301 atcgccgcca gggggataag cgccgcaaat gcccttccca gcgcccggat cccgccggtg
      361 agccagtttc cgagtcgccg cgcccactcg gccgcgccct taatcgcgcc gctgtcatcg
      421 aagttcgcag tgggcttcat ctcctcgatc agcgacggca gcactactag cagggcggcg
      481 gcgaaggcga gcgcgaagcc ggcgacgagg ggcgtgaaga agccgccgag ggcctgcacc
      541 cggtcgtcga gaaagaacgc ggccgaccgg tagccgcggc cgaaaaaatg cggcaggtag
      601 agaaaatccg agagcgcgcg cccgacgatg tagcttacga ccgaccacaa cacgaggctg
      661 agcaccgcaa agagcccggt ggagccgatg agcgcgagcc gcgccgtgtg aagcgacgcc
      721 cgggccgctt tgtcgcagcc gcggcctagc cagagtccaa gcgtgagggc gctaatctgt
      781 accgcgacga agacggccca cacgatcagc acaccgccat aaagcacttc acctgcgcta
      841 agggcgctga gaagcatcca ctcgatttcc gtagtggggc gcacgagctg gccggcgacc
      901 cataccatca ggacagtcgc caccacgatc agatgaccga gcagccgaac accccgcgtg
      961 acaccggaat agcgctcgat gagataccca cccagcaggc cgatgcccaa cgcgctggcc
     1021 acgaagtaaa cgaccggcgc tacacctgcg gcagcgagag ccaaaaccgc gaacgccaga
     1081 ctgaaggacg cgaccgcgaa gagaaacgcg agcttcgccc agcgcgcaaa cccgggcgcc
     1141 gcacgcaacc aggcaagcac tgcgagcgcc gccagcacaa cacctccgag cccgaaaatc
     1201 gcagcgacaa gttgtccccg aaggtcggga tgaaccaagg ctgctgcgcc gaacacgatt
     1261 aagagcagca tggcgagttg caataacgcg gccggcccct tcatgatcca cgccatccag
     1321 gcgtgaaggc gctgcagcag gcgccaggcc cttcccccgt tggtgctgag cgacacctgg
     1381 tcgaccacgt cggccgcaag cgtgctcagg tgaaagaaca gctggtagag cgacgacagc
     1441 gcgcgcaggc cgccggtgcc gaggcgcgac agatcagccc aatacatctc gtacacgtcg
     1501 agcgggcgtc catccgcgcg gcggcggagc cgaacccgcg tggactcgta gagcgcatcg
     1561 ctctccggca gcacgaagcg gctgagcaag taatcgttga gcgccatgcc gagatcctgc
     1621 gtctgcggcg cccgttgctt cggctcttgt gccttcacct cggccgactg ctgcgcttga
     1681 tagaacccgc tcggcgtccc cgggcgctgg cgcggcgagg catcctgcag gacacggtct
     1741 cccggcggca acggttcgac cggtaccagc acatcctgca tctcgccctg cacgtaggag
     1801 gccctgtcag gccctcctcg acagaggagc cgcgccactt cccgcaccgt ctggcgcgga
     1861 cgctggtcgg cgataccgtg cgccacgacg atggcgacgc gatcggtcgc gctcattgac
     1921 cgatcaccat cgagctactc cggcatgcgg catttgttct tgtgtgcgtt cttccagtgc
     1981 actgtatgcc cgcaccggag acgggtcaac gcaaacaccg acggtgaaag acggcacgct
     2041 gctcgtcttc ccttcgtatc tgtatcaat
//
LOCUS       DASTPD010000318         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_169533, whole genome shotgun sequence.
ACCESSION   DASTPD010000318 DASTPD010000000
VERSION     DASTPD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169533"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /gene="lon"
                     /locus_tag="VFR57_10555"
     CDS             complement(<1..1670)
                     /gene="lon"
                     /locus_tag="VFR57_10555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU0259842.1"
                     /translation="MNDKAVAAAPTKPQAVKPLPEDALIVIPMRNTVLFPGVIAPVTV
                     GRATSVAAAQEAVRSEKKVGFLLQRDPQKNDVKPEDLYWVGTAGQVVRYITGAEGTHH
                     LVVQGQSRFRALEFLQGWPFLVARVELVPQKEEMNPELEARFLQLKSQAVEAISLLPS
                     APEELTVVVQGMSSPALLADMVANLLDIKNEDKQDLLETFDLKRRLDKVLEHLSERVG
                     VLRISKEIGEKTKKEFDERQREHVLREQMRQIQKELGEGGDNAAELEELAKAIEEAGM
                     PEDVHKHAVKELKRLQRMGEGSAEGSMLRTYLEWLAELPWKISDTKEIDIAQARKVLD
                     EDHFGLDKIKRRILEHLAVRKLNPEGKSPILCFVGPPGVGKTSLGQSIARATGRKFQR
                     VALGGVHDEAEIRGHRRTYIGALPGNVIQAIRRAESRTCVLMFDEVDKLGMGGFHGDP
                     SSALLEVLDPEQNSHFRDNYLAVDFDLSKVMFITTANVLDTIPGPLRDRMEIIQLPGY
                     TEEEKLEIAKRYLVKRQLEANGLKAEQVELPEETLRAIIADYTREAGVR"
     gene            complement(1667..2077)
                     /locus_tag="VFR57_10560"
     CDS             complement(1667..2077)
                     /locus_tag="VFR57_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0259843.1"
                     /translation="MRSRDFSSWMWGEALSMLDRAQRLQRQFFTHAEVAWEPPVDIVE
                     TGDTLQMHIALPGVSADAITVALDSAGITISALRAFPCRGEDSQIHRLEIPYGRFERF
                     IGLPLGDPYSPLELVAKSLQDGILTLTFQRKESP"
     gene            complement(2109..>2319)
                     /locus_tag="VFR57_10565"
     CDS             complement(2109..>2319)
                     /locus_tag="VFR57_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0259844.1"
                     /translation="EAASQKLGVALALRPLVNADIASGRLVAPFEIEVKPGSAYYLVC
                     PEVIADRPAVAAFRKWLLEQAKNSP"
BASE COUNT          342 a          765 c          784 g          428 t
ORIGIN      
        1 cgcacgccgg cctcgcgggt gtagtccgcg atgatggcgc gcagcgtctc ttccggcagc
       61 tctacctgct ctgccttcag gccgttcgcc tcgagctgcc gcttgaccag gtagcgcttg
      121 gcgatctcga gcttctcctc ctcggtatag ccggggagct ggatgatctc catgcggtcg
      181 cgcagcggcc cggggatcgt gtcgagcacg ttcgcggtgg tgatgaacat caccttcgag
      241 aggtcgaagt cgaccgcgag gtaattgtcg cggaagtgcg agttctgctc cgggtcgagc
      301 acttccagca gcgcgctcga cgggtcgccg tggaagccgc ccatgccgag cttgtcgacc
      361 tcgtcgaaca tcagcacgca ggtgcgcgac tcggcgcggc ggatcgcctg gatcacattg
      421 ccgggcagcg cgccgatgta ggtgcgccgg tggccgcgga tctcggcctc gtcgtgcacg
      481 ccgccgagcg ccacacgctg gaatttgcgt ccggtcgcgc gcgcaatcga ctggccgagc
      541 gaggtcttgc ccactcccgg cgggccgacg aagcacagga tcgggctctt gccctcgggg
      601 ttgagcttgc gcaccgcgag gtgctcgagg atgcggcgct tgatcttgtc gaggccgaag
      661 tggtcctcgt cgagcacctt cctcgcctgc gcaatatcga tttcctttgt gtccgaaatc
      721 ttccacggga gctcggccag ccactcgagg taggtgcgca gcatcgagcc ctcggcgctg
      781 ccctcgccca tgcgctgcag gcgcttgagc tccttgacgg cgtgcttgtg cacgtcctcg
      841 ggcatgccgg cttcctcgat cgctttcgcg agctcttcga gctcggccgc gttgtcgccg
      901 ccctcgccca gctccttctg gatctggcgc atctgctcgc gcagcacatg ctcgcgctgg
      961 cgctcgtcga actccttctt ggttttctcg ccgatctcct tggagatgcg cagcacgccg
     1021 acgcgctcgg aaaggtgctc gagcaccttg tcgagacggc gcttgaggtc gaaggtctcg
     1081 agcaggtcct gcttgtcctc gttcttgatg tcgagcaggt tggccaccat gtcggcgagc
     1141 agcgcgggcg agctcatgcc ctgcacgacg accgtgagct cctcgggcgc gctcggcagg
     1201 agcgagatcg cctcgaccgc ctgcgatttg agctgaagga agcgcgcctc gagctcgggg
     1261 ttcatttcct ccttttgcgg gacgagctcg acccgggcca caaggaacgg ccagccctgc
     1321 aggaactcga gcgcgcggaa gcggctctgg ccctgcacca cgagatgatg cgtgccctcg
     1381 gcgccggtga tgtagcgcac cacctggccg gccgtgccga cccagtagag gtcctcgggt
     1441 ttgacgtcgt tcttctgcgg gtcgcgctgg agcaggaagc ccaccttctt ctcgctgcgc
     1501 accgcttcct gcgcggccgc gaccgacgtg gcgcggccca cggtgacggg cgcgatcaca
     1561 cccgggaaca gcaccgtgtt ccgcatcggg atgacgatca gcgcgtcctc gggcagcggt
     1621 ttcaccgcct ggggcttcgt gggggcagcg gcaacagcct tgtcgttcat ggactctcct
     1681 tccgctggaa ggtgagggtg aggatgccgt cttgcaacga cttcgccacg agctcgagcg
     1741 gcgagtacgg gtcgccgagc ggaaggccga tgaagcgctc gaagcggccg taagggatct
     1801 ccaggcgatg gatctggctg tcttcgccgc ggcaggggaa agcgcgcagc gccgagatgg
     1861 tgatccctgc ggaatcgagc gcgacggtga tggcgtcggc gctcacgccg ggaagcgcga
     1921 tgtgcatctg cagcgtgtcg ccggtctcga cgatgtcgac gggcggctcc cacgccacct
     1981 cggcgtgggt gaaaaactgg cgttgcaggc gctgcgcccg gtcgagcatc gacagggctt
     2041 cgccccacat ccagcttgag aagtcgcgcg atctcaccgc ggttagatgg cggcgaatcc
     2101 cctcgggttc aaggggagtt tttagcctgc tccaataacc acttgcgaaa ggcggcgacc
     2161 gccggccggt ctgcgatgac ctctgggcac acgaggtaat aggccgaccc cggcttcact
     2221 tcgatctcga acggcgcgac gagcctgccg gaggcgatgt cggcgttcac gagcgggcgc
     2281 aacgcaagcg ccacgccgag cttctgcgac gccgcctcg
//
LOCUS       DASTPD010000319        12536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_987690, whole genome shotgun sequence.
ACCESSION   DASTPD010000319 DASTPD010000000
VERSION     DASTPD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987690"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFR57_10570"
     CDS             <1..810
                     /locus_tag="VFR57_10570"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0259845.1"
                     /translation="YFLPTGRWGQRMGDAAVVDAMTGALHDPFGHGHMGVTAENIAAK
                     YGFTRAQQDEFALASHKKAAKAIETGYFKSQIVPIELKTRKGVEQFTTDEHVRKDAKP
                     EDMAKLKAVFKKDGTVTAGNASGINDAGAAVVLVSSSFADKNNMKPMARLVAYAHAGV
                     DPKVMGLGPIPAVKRVFEKAGLKPSDMDVIESNEAFAVQAMAVTKDLGLDPAKVNPNG
                     GAVALGHPIGATGAILTLKALYELERTQKRYALVTMCIGGGQGIAAIFERL"
     gene            826..1725
                     /gene="rssA"
                     /locus_tag="VFR57_10575"
     CDS             826..1725
                     /gene="rssA"
                     /locus_tag="VFR57_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEU0259846.1"
                     /translation="MRRKLRLGLALGSGSARGWAHIGVIRALEERGIKADMVCGSSIG
                     ALVGAAYASGELERLEKWACSLTWATVVRLMDLTWRGGLIRGTRLFALFRALLEDREI
                     DELPLPFGAVATELASGRELWLRHGKVLDAVRASCAMPGLLTPVVREGAVLVDGGLVN
                     PVPVSMCRALGADLVVAVDLSWGKLGPYRRSKETELVPREVPGWLDRLRPLIASDWLK
                     SKQKPEETPDIPSLFNVFMTSLDIVEMRVARSRLAGEPADVLITPLLPDFATMDYHRA
                     REAIAEGRAAVDRMGPLLEQVLS"
     gene            complement(1722..3173)
                     /gene="lysS"
                     /locus_tag="VFR57_10580"
     CDS             complement(1722..3173)
                     /gene="lysS"
                     /locus_tag="VFR57_10580"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU0259847.1"
                     /translation="MEEENKLVDERREKLKAVRGAGQAFPNDFRRSHFAGDLAKRFGE
                     RTKEELEKTRVEVKVAGRMMLKRVMGKASFATLQDGSGRIQIYVVNEVKNYEDFKHWD
                     LGDILGVEGHLFKTMKGELTIHARSIRLLVKALRPLPEKFHGLADQELRYRQRYVDLI
                     VNPQVKDVFGKRIEIVRTLRETLFADGYVEVETPMMQPIPGGAAARPFKTHHNALEMD
                     LYLRIAPELYLKRLVVGGMERVFEINRNFRNEGISTKHNPEFTMLEWYCAYEDWRYMM
                     DHTERLVRAAAGNAVVTYQGKRLDFSKPFARLSIPDAIRKEGVQGDLRDRKLLSEKLD
                     SLNIQYEKHQGWGALQLMLFETIAEKRLIEPTFITDFPAEVSPLARRRDGDLEVADRF
                     ELFIDAKEIANGFSELNDPEDQAARFLEQVRRREAGDQEAMYYDADYVRALEYGMPPC
                     AGGGLGIDRLVMLLTDSPSIRDVILFPHMRPEG"
     gene            complement(3175..4279)
                     /gene="prfB"
                     /locus_tag="VFR57_10585"
     CDS             complement(join(3175..4197,4199..4279))
                     /gene="prfB"
                     /locus_tag="VFR57_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU0259848.1"
                     /translation="MEAERINQIASRLADLQARSAELRRYLDFEEKQKRLAALDSEFE
                     NPKLWDNPQRAQELGKEKKALETVVGTLTRVGTGLADAAELFGLARAEGDDATLAGVD
                     SDVQKLENDVAAMEFRRMFSNPLDPNNCFIDIQAGSGGTEAQDWASMLERMYLKFCDR
                     RGFSAEVLEHTPGEVAGIKSATLKVRGDYAYGTLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDESIEVEVNPADLRIDTYRASGAGGQHVNRTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMAMLKAKLYELEMRKRQAEKQKLEETKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGATQAVLDGELDDFIEASLKQGV"
     gene            complement(4279..4761)
                     /locus_tag="VFR57_10590"
     CDS             complement(4279..4761)
                     /locus_tag="VFR57_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259849.1"
                     /translation="MVRGLLLILCLATSVPGAAAPQRVLPPSEPVFAFDSLHGIAAWL
                     RHYGGEGYIGADVADAMGIARPQGEALLAARQRGFRSDDVLRIAQISADGERDFILFM
                     VQWPDDRVYFYLSTPLGGLQKAFLSIPSRNLVVPLERGEAEVRFRQEVLYWKDRSEAR
                     "
     gene            complement(4787..4996)
                     /locus_tag="VFR57_10595"
     CDS             complement(4787..4996)
                     /locus_tag="VFR57_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEU0259850.1"
                     /translation="MQRKRVNSSKIRSVGYDEATRTLEVEMSNGQVFQYSGVYPEVHR
                     RFMAAPNPTSFFDDKIAEEYTAKRV"
     gene            complement(4998..5180)
                     /locus_tag="VFR57_10600"
     CDS             complement(4998..5180)
                     /locus_tag="VFR57_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259851.1"
                     /translation="MKLRISAGGASFEVVVYGRTPISTDERPKLAARCNVVGRLENPA
                     ALANIRSGEKISVSRA"
     gene            complement(5177..6016)
                     /locus_tag="VFR57_10605"
     CDS             complement(5177..6016)
                     /locus_tag="VFR57_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0259852.1"
                     /translation="MIKNFLAGTALAFSGFALAADVQPLKDAGPYVPSPQSVVSDMLR
                     YADVGANDFLIDLGSGDGRIVLTAAKVFGARGFGVEIKEDLVRLSNETAQKEGVADRV
                     KFLKQDLFKTDMSQATVITMYLLPDTVNLLKDKFLNELRPGTRIVSHDYPLTGWIPDK
                     YVQMDLEDKVQISGVTTTLIYLYVVPAKVAGKWSAQMPPAVSRQPATLSLKQQLTRVS
                     GSMRLDGKDVALEAVRLRGDRLSFRVVGRQGEFSGQVKGNQIEGIVDRGGTKLPWSAN
                     LGG"
     gene            complement(6013..7698)
                     /gene="recJ"
                     /locus_tag="VFR57_10610"
     CDS             complement(6013..7698)
                     /gene="recJ"
                     /locus_tag="VFR57_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU0259853.1"
                     /translation="MRIVERPYVEASRRALLDAGLHPVLARVYAGRNIHSPAQLEYQL
                     GALLPPHSLKGIDSAATLLANAIAHSRRLLIISDYDADGATACAVGVRALKAFGANVD
                     YLVPDRFKLGYGLSPELVDVAAQRKPDLLITVDNGIASVEGVARARSLGMATLITDHH
                     LPGAELPAAECIVNPNQPGCAFPSKALAGVGVMFYVMLALRAELRTRNHFRGREEPNL
                     ASLTDLVALGTIADVVPLDANNRNLVAQGLRRLRGGRCTPGIAALLRAAGRPLVEASS
                     FDLGFVAGPRLNAAGRLADMSLGIECLITDDEARAANCAQELERLNRERRGIEASMLQ
                     QALLAIERVSSSEGCTFFHPDWHQGVVGIVASRLKDRLHRPVICFARAEEAGEIVLRG
                     SGRSIPGLHLRDALDAVSKRAPGLIRRFGGHAQAAGLSIAAQDYERFAGEFARTVDEL
                     LPAQARLRVVETDGALDAMHFSLEVARLLDAGIWGQGFPQPLFCDTFIVENQRVVGER
                     HLKLWLVKGGRRIEAMRYGALDPLPPQVRAAYRLSVNEFNGLKNIQLNVEHYE"
     gene            complement(7695..8657)
                     /locus_tag="VFR57_10615"
     CDS             complement(7695..8657)
                     /locus_tag="VFR57_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259854.1"
                     /translation="MHCELVVPGLFASGGTSRFAALELLLARGRRSAGEAKALECWVQ
                     EAFELGEGVYPAGALTLVAQGIDPGADSWARADPVHLRVMDERLIVLPAETFALRAEE
                     SRALCEALNAHFAGMEFGMAQPRRWYVKLGQIEEGRPALDAAGTALELPKQMAPLLNE
                     IQMVLHFHPVNEAREARGEPPVNSLWLWGAGRAPRTRAPWQSVAADDPAVLGAARLAN
                     ARQRALPRSAGEWLERLPEDGRHLAVLDALRAPLALAADTVQNEMQALERDWFAPLLA
                     ALRRGRIGMVTLHVPDAAEALSFEIIRGDLRRFWRRPRSIKNYG"
     gene            complement(8657..10024)
                     /locus_tag="VFR57_10620"
     CDS             complement(8657..10024)
                     /locus_tag="VFR57_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU0259855.1"
                     /translation="MQARLAIVDVETTGSDPAGDRITEIAVLQAEGGALTAQWSTLVN
                     PGRPIPGVIQALTGISPQMVGAAPRFAEIAGELAERLAGRVLVAHNARFDYGFLRREF
                     ERAGINYRAKTLCTVRLSRRLYPDTGGHDLDSLIERHRLGCAPRHRALPDAAALWQFL
                     RIAAEEHGDEVVDVAARQIVRAPTLPPQLDPSSIDEIPDAPGVYLCYGEGAAPLYIGK
                     SRALRSRVLQHFIAGASWTPRVRRIEWQRTAGELGALLREAQLVKALDPTYNRKLRRP
                     ERLCGFAFDGKRLRLAGEDEIDAETLPLVYGVWRSRRAALQALRATADAHRLCLQVLG
                     VEHALARPGPCFRYQIGRCAGVCAGKESIHAHHGRLAAALAPLKSPDWPHRGPLGVVE
                     ADASRDATEVHILDRWCYLGTATSEAEVADLLEARRPRFDYDHYRILSRHLGKRGVRV
                     VPLWA"
     gene            10080..10409
                     /locus_tag="VFR57_10625"
     CDS             10080..10409
                     /locus_tag="VFR57_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259856.1"
                     /translation="MRAIALLGLVLAAGCTPMQWQKPGITAEQLDADEEDCRQRAWSE
                     ASLYAWQYQTMWGPVFPRDSYGRGAMVWPGSPMADPYGYQMMEETRLAQFCMEAKGYK
                     LVPVPKE"
     gene            complement(10410..11222)
                     /locus_tag="VFR57_10630"
     CDS             complement(10410..11222)
                     /locus_tag="VFR57_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEU0259857.1"
                     /translation="MRIYGVDFTCAPRRAKPITVAAGILKKKLLKLEGLEKLESFPAF
                     ERFLARPGPWVGGFDFPFSLPRELVRDLGWPHDWQGLVEHCAAMDRLAFRATLDVYRA
                     SRPAGSKYVHRATDHPAGSSSPMKLVNPPVALMFHEGARRLAACDVHIPALRNRRSSR
                     VALEAYPGLLIRKQLGIRASYKSDTRSEHTAARALIRRAILRSLKAGQPLGIRVALEP
                     ALARALVADGSGDLLDAAICAVQAACSWTRRRSNYGLPADAPAGEGWIVTAG"
     gene            complement(11219..12070)
                     /locus_tag="VFR57_10635"
     CDS             complement(11219..12070)
                     /locus_tag="VFR57_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0259858.1"
                     /translation="MDLVELQIFKTVAEQGGITRAATALHRVQSNVTTRVKQLEERLG
                     AKLFHRQGRKLVLSSEGRLLLGYAERLLRLSTEAQAAVKGHTLHGLFRLGSLESTAAA
                     RLPPVLAAYHRAHPGVRLELVTGTSGALVDKVLRGDIEAAFVAEPFATKNLEMQHVFT
                     EELVLITPRSHSRVTTAKELEQRVVLAFPTGCSYRRRFESWLGRASVVAERVMEYGSY
                     HAIIACVAAGGGIAVVPRSVLRVVGAENQLAMHALPQQVSKARTMLVWPRGHQSAALA
                     ALRKALD"
     gene            12162..>12536
                     /locus_tag="VFR57_10640"
     CDS             12162..>12536
                     /locus_tag="VFR57_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU0259859.1"
                     /translation="MPIRTALTESLGIRHPIVLAPMDLVSDARLTAAVSAAGGLGLLG
                     GGYGDERWLRCELDLLERSKARFGVGFITWSMARQPKLLDLALERKPVAVMLSFGDPA
                     PFADRIKRAGARLLCQVQTLAMA"
BASE COUNT         2038 a         4145 c         4177 g         2176 t
ORIGIN      
        1 tacttcctgc cgacgggtcg atggggacag cgcatgggcg acgcggcggt agtggatgca
       61 atgaccggcg cgctgcacga cccgttcggt cacggccaca tgggcgtgac cgcggaaaac
      121 atcgcggcga agtacggctt cacgcgcgcg cagcaggatg aatttgcttt ggcttcacac
      181 aagaaagccg ccaaagcgat cgagacggga tacttcaagt cgcagatcgt tccgatcgag
      241 ctcaagacgc gcaagggcgt cgagcagttc acgacggacg agcacgtgcg caaggacgcg
      301 aagcctgagg acatggcgaa gctgaaggcg gtgttcaaga aagacggcac ggtcaccgcg
      361 ggcaacgcct ccggcatcaa tgatgcgggc gcggcggttg ttctggtttc ctcttctttt
      421 gcagataaga acaacatgaa gccgatggca cggctagtgg cgtacgcgca cgccggcgtt
      481 gatcccaagg tcatggggct tggcccgatt ccggcggtaa agcgggtgtt cgagaaagct
      541 ggcctcaagc cctccgacat ggacgtcatc gagtcgaacg aagcatttgc ggtgcaggcg
      601 atggcggtga cgaaggacct cggcctcgat ccggcgaagg tgaacccgaa cggcggggcg
      661 gtggcgctcg gtcacccgat cggcgccacc ggcgcgatcc tcacgctgaa agcgctctac
      721 gagctcgagc gcacgcaaaa gcgctacgcg ctagtcacca tgtgcatcgg cggcggccag
      781 ggaatcgcgg cgatcttcga gcgcctctga cttaaactgc cgcgcatgcg gcgcaagctc
      841 cgcctcggac tcgcgctcgg ctcgggcagc gcgcgcggct gggcgcacat cggcgtgatc
      901 cgcgcgctgg aagagcgcgg gatcaaggcc gacatggtat gcggcagctc gatcggcgcg
      961 ctggtggggg cggcctatgc ttcgggagag ctcgagcggc tggagaaatg ggcctgcagc
     1021 ctcacctggg ccaccgtggt gcgcctgatg gacctgacgt ggcgcggcgg gctgattcga
     1081 ggcacgaggc ttttcgcgct gttccgtgcg ctgctcgagg atcgcgagat cgacgagctg
     1141 cccctgccgt tcggggcggt cgccaccgag ctcgcctcgg gcagagagct ctggctgcgc
     1201 cacggcaagg tgctcgatgc ggtgcgtgct tcctgcgcca tgcccgggct gctcacgccg
     1261 gtggtgcgcg agggcgcggt attggttgac ggcgggctcg tcaatccggt gccggtgtcg
     1321 atgtgccggg ctctcggcgc cgatctggtg gtcgcggtcg atttgagctg gggaaagctc
     1381 ggtccttacc gccgcagcaa ggaaaccgag ctcgtgccgc gcgaggtgcc ggggtggctt
     1441 gaccgtctgc ggcccctcat cgcctccgat tggctgaaga gcaagcagaa gccagaggaa
     1501 acaccggaca tcccgtcgct cttcaacgtg ttcatgactt cgctcgatat cgtcgagatg
     1561 cgcgtagcgc ggagccgcct cgccggcgag ccggccgacg tgctcatcac gccgctgctg
     1621 ccggacttcg ccaccatgga ctatcaccgc gcgcgcgagg cgatcgccga gggccgcgcg
     1681 gcggtcgacc gcatgggccc ccttctcgag caggtattga gctagccctc gggccgcatg
     1741 tgcgggaaga gaatcacgtc ccggatgctc gggctatcgg tgagcagcat caccaggcgg
     1801 tcgataccca ggccaccgcc ggcgcacgga ggcatgccgt attcgagcgc gcgcacgtag
     1861 tcggcgtcgt agtacattgc ctcctggtcc cctgcctcgc gcctgcgcac ttgctcgagg
     1921 aagcgcgcgg cctggtcctc ggggtcgttc agctcggaga atccgttcgc gatctccttc
     1981 gcgtcgatga agagctcgaa gcggtccgcc acctccaggt cgccgtcgcg ccggcgcgcg
     2041 agcggcgaca cttcggcggg gaagtcggtg atgaaggtgg gctcgatcag acgcttctcg
     2101 gcaatcgtct cgaacagcat gagctgcagc gctccccagc cctgatgctt ttcgtattgg
     2161 atgttcaatg aatcgagctt ttcggaaaga agttttcggt cgcgcaagtc gccctgcacg
     2221 ccttccttgc ggatcgcatc gggaatggag agccgcgcga aaggcttgct gaaatccagg
     2281 cgcttgccct ggtaggtgac gacggcgttg ccggccgcgg cgcgcaccag ccgctcggtg
     2341 tggtccatca tgtagcgcca gtcctcgtag gcgcagtacc actcgagcat cgtgaattcg
     2401 gggttatgct tggtcgagat gccctcgttt ctgaaatttc ggttgatctc gaacacgcgc
     2461 tccattccgc cgaccaccag gcgcttgagg tagagctcgg gcgcaatacg aaggtaaaga
     2521 tccatttcga gcgcgttgtg gtgggtcttg aatggccgcg ccgcggcgcc gccgggaatc
     2581 ggctgcatca tcggcgtctc gacctcgaca tagccgtctg caaacagcgt ctcgcgcagc
     2641 gtgcgaacga tttcaatcct tttcccgaaa acatccttca cctgcgggtt gacaatcagg
     2701 tctacatagc gctgtcggta gcgcagctcc tgatcggcaa ggccgtggaa cttctcgggt
     2761 agcggccgca gcgccttcac cagcagccgg atgctccgcg cgtggatcgt cagctcgcct
     2821 ttcatcgtct tgaagaggtg accttccacg ccgaggatgt cgcccaggtc ccagtgcttg
     2881 aagtcctcgt agttcttgac ttcattcacc acatagatct ggatccggcc gctgccgtcc
     2941 tgcaacgttg cgaagctcgc cttgcccatc acgcgcttga gcatcatgcg acccgcaacc
     3001 ttgacctcga cgcgcgtttt ctcgagttcc tccttggtcc tctcaccgaa gcgtttcgcg
     3061 aggtcgccgg cgaagtgaga gcgccggaag tcgttgggga acgcctgccc ggcgccgcgc
     3121 acggccttca gcttctcgcg ccgctcgtcg accagcttgt tttcttcttc caaactagac
     3181 tccttgcttg aggctcgctt cgatgaagtc gtcgagctcg ccgtcgagca ccgcctgggt
     3241 cgcgcctttc tccacgccgg tgcgcaggtc cttgatgcgc gactggtcga gcacgtacga
     3301 gcggatctgg tgtccccagc cgatgtcggt cttcgtctcc tcgagcttct gcttctcggc
     3361 ctggcgcttg cgcatctcga gctcgtagag cttcgccttg agcatcgcca tcgcctcggc
     3421 gcggttgcgg tgctgcgagc ggtcgttctg gcactgcacc acgatgttcg tcggcaggtg
     3481 tgtgatgcgc accgcgctgt cggtgcggtt cacgtgctgg ccgcccgccc cggaagcgcg
     3541 gtaggtgtct atgcgcagat ccgccggatt aacctcgacc tcgatcgact cgtcgacctc
     3601 ggggtagacg aagacgctgg caaacgaggt gtgtcggcgg gcattggcgt cgaacgggct
     3661 cttgcgcacc agacggtgca ctccagtctc ggtgcgcagt gtgccgtacg cgtagtcgcc
     3721 cctcaccttg agcgtcgcgc tcttgatacc cgcaacttcg cccggggtgt gctccagcac
     3781 ctctgcgctg aacccccgcc ggtcgcagaa cttgaggtac atgcgctcca gcatcgacgc
     3841 ccagtcctgc gcctcggtgc cgcccgagcc ggcctggata tcgatgaagc agttgttcgg
     3901 gtcgagcggg ttcgagaaca tgcgccggaa ctccatcgcg gcgacgtcgt tttcgagttt
     3961 ctgaacatcg gaatccactc cggcgagcgt agcgtcgtcg ccctcggcgc gggcgaggcc
     4021 gaagagctcc gcggcatcgg caaggccggt tcccacccgg gtgagagtgc cgactacggt
     4081 ttcgagagcc ttcttctctt tgccgagctc ttgcgctcgc tgcgggttat cccaaagctt
     4141 cggattctcg aattcgctgt ctagcgcagc gaggcgtttc tgcttctctt cgaagtcaaa
     4201 gatacctccg cagctccgcc gaccgcgcct gcaggtcggc gagccgggag gcgatctggt
     4261 tgatccgctc ggcttccatt agcgcgcctc gctccggtcc ttccagtaga ggacttcctg
     4321 ccggaagcgc acttccgcct cgccgcgctc gagcggcacc accaggttgc ggctagggat
     4381 ggagaggaac gccttctgca ggccgcctag cggtgtggac aggtagaagt acacccggtc
     4441 gtcgggccac tgcaccatga acaggatgaa gtcgcgctcg ccgtcggccg agatctgcgc
     4501 gatgcgcagc acgtcgtcgc tccgaaagcc gcgctgccgg gcggcgagca acgcctcgcc
     4561 ctgcggccgc gcaatcccca tggcatcggc gacatccgcg ccgatatagc cctcgccccc
     4621 gtagtgccgc agccaggcgg cgataccgtg caggctgtcg aacgcgaaca cgggctcgct
     4681 cggcggcagg acgcgctgcg gcgccgcggc tcccggcacc gacgtggcaa ggcacagtat
     4741 tagcagcagc ccccgaacca tcttcgttgt ctcccttgcg ggtagtctac acgcgcttcg
     4801 cggtgtattc ctcggcgatc ttgtcgtcga agaaggaagt tggattgggc gccgccatga
     4861 agcgccggtg gacctcggga tagacgccac tgtactggaa cacctggccg ttcgacatct
     4921 cgacttccag cgtgcgcgtg gcctcgtcgt agcccaccga gcggatcttg gaagagttga
     4981 ctcgcttgcg ctgcatatca ggccctgctg accgaaattt tctcgccgga ccggatgttc
     5041 gcgagggctg ccggattttc cagacggccc actacgttgc agcgggcagc gagcttgggc
     5101 cgctcatcgg tggagatcgg cgtgcggccg tacacgacga cctcgaagct cgcgccgccg
     5161 gcgctgatgc gcagcttcat ccgccgagat tcgcgctcca aggcagcttg gtgccgcctc
     5221 tgtcgacgat gccttcgatc tggtttcctt tgacctgacc gctgaactcg ccctggcggc
     5281 cgacgaccct gaagctcagg cggtcgccgc gcagcctgac ggcttccagc gcgacatcct
     5341 tgccgtccag gcgcatgctg cccgacacgc gagtgagctg ctgcttgagc gacagcgtcg
     5401 ccggctggcg gctcaccgcg ggcggcatct gcgcgctcca tttccccgcg acctttgccg
     5461 gtaccacgta caggtagatc agcgtcgtgg tgacgccgct gatctgaacc ttgtcctcga
     5521 gatccatctg cacgtatttg tcaggaatcc agccggtgag cgggtagtcg tgggacacga
     5581 tgcgtgtgcc ggggcgcagt tcgttcagga acttgtcctt gagcaggttc accgtgtccg
     5641 gcagcaggta catcgtgata acggtggcct gcgacatgtc ggtcttgaaa agatcctgct
     5701 tcaggaactt gacgcggtcg gcgacgcctt ccttctgcgc ggtctcgttc gacaggcgca
     5761 ccagatcctc cttgatctcg acgccgaagc cgcgcgcgcc gaagaccttc gccgcggtca
     5821 gcacgatgcg gccgtcgccg gagccgaggt cgatgaggaa atcgttcgcg cccacgtcgg
     5881 catagcgcag catgtccgac accacgcttt gcggcgaggg cacatagggc ccggcatcct
     5941 tgaggggctg cacgtccgcg gcaagcgcaa atccggaaaa cgcgagcgcc gttcccgcca
     6001 gaaaattctt gatcattcgt aatgctccac gttgagctgg atgttcttca ggccattgaa
     6061 ctcgttgacg ctcagccggt aggccgccct gacctgagga gggagcggat cgagagcgcc
     6121 gtagcgcatc gcctcgatgc ggcggccgcc tttcaccagc cagagcttca agtgccgctc
     6181 gccaacgacg cgctggtttt ccacgatgaa tgtatcgcag aagagaggct ggggaaagcc
     6241 ctggccccag atgccggcgt cgagcaggcg cgccacctcg agcgaaaagt gcatggcgtc
     6301 gagcgcgccg tcggtttcca cgacgcgcag ccgcgcttgc gccggcagca gctcgtcgac
     6361 cgtgcgagcg aactcgccgg cgaagcgctc gtagtcctgt gccgcgatgc tgagccctgc
     6421 ggcctgggcg tggccgccga agcggcggat gagccccggc gctcgcttcg acactgcgtc
     6481 gagcgcatcg cgcaagtgca gccccgggat cgagcgtccg gagccgcgca gcacgatctc
     6541 gcccgcttcc tctgccctcg cgaagcagat caccggccgg tgcagccgat ccttcaggcg
     6601 ggaagccacg attccgacca cgccttggtg ccagtctgga tggaagaaag tgcagccttc
     6661 cgaagaagag actctctcga ttgcgagcaa cgcctgttgc agcatcgagg cttcaatgcc
     6721 gcgccgctcg cggttcaggc gctcgagctc ctgcgcgcaa ttcgcggcgc gcgcctcgtc
     6781 gtcggtgatc aggcattcga tgccgaggct catgtcggcg agccgcccgg cggcgttcag
     6841 gcgcgggccg gcgacgaagc cgaggtcgaa gctcgacgct tcgacgagcg gccggccggc
     6901 cgcgcgcagc aacgccgcta ttccgggcgt gcagcggccg ccgcgcaggc gcctgagccc
     6961 ctgggccacg aggttgcggt tgttcgcgtc gagcggcacg acgtccgcaa tggtgccgag
     7021 cgccacgagg tcagtgaggg atgcgaggtt cggttcttct cttcccctga agtgatttcg
     7081 tgttcgaagc tcggcccgca acgcgagcat tacgtagaac atcactccga cgccggcgag
     7141 cgccttggag gggaatgcgc agccgggctg gttggggttg acgatgcact cggcggcggg
     7201 aagttccgcc ccgggaaggt gatggtcggt aatcagagtt gccatgccga gactgcgggc
     7261 gcgtgccacg ccctcgacgc tcgcgatgcc gttatcgacc gtaatgagca gatcgggctt
     7321 tctctgcgcc gcgacgtcga ccagctcggg cgagagcccg tagcccagct tgaagcggtc
     7381 cgggaccagg tagtcgacgt ttgcaccaaa agccttgagt gcccgaacgc ccacagcgca
     7441 cgccgtcgcg ccgtcggcat cgtagtcgga gatgatgagc aggcgcctcg agtgcgcgat
     7501 ggcgttcgcg agcagcgtcg ccgcggaatc gatgcccttg agcgaatgtg ggggtagcaa
     7561 agcgccgagc tggtactcga gctgcgccgg cgaatggatg ttcctgccgg cgtacacgcg
     7621 cgcgagcacc ggatgcagcc ctgcgtcgag cagcgcgcgg cggctcgctt cgacgtaggg
     7681 tcgctcgacg attctcatcc gtagttcttg atcgatcgcg ggcgccgcca gaaacgccgc
     7741 aagtcgccgc gaatgatctc gaagcttaac gcttcggcgg cgtcgggcac gtgcagcgtc
     7801 accatgccga tccgcccgcg gcggagcgcg gcgagaagcg gcgcgaacca atcgcgctcg
     7861 agcgcttgca tttcattctg gactgtgtcg gctgcgagcg cgagcggggc ccgcagcgca
     7921 tcgagcaccg cgaggtggcg gccgtcttcc ggcaggcgct cgagccattc ccctgccgag
     7981 cgcggcagcg cgcgctggcg cgcgttggcg agtcgcgcag ccccgagcac cgccgggtcg
     8041 tccgcggcca ccgactgcca cggtgcgcgc gtgcgcgggg cgcgccccgc tccccatagc
     8101 cagaggctgt tcaccggggg ctcgccgcgc gcttcgcgcg cttcattgac cggatggaag
     8161 tgcagcacca tctggatctc gttgagcaac ggtgccatct gcttcggcag ctcgagcgcc
     8221 gtgccggcgg catcgagggc ggggcggcct tcttcgatct gcccgagctt cacgtaccag
     8281 cgccgcggtt gcgccatgcc gaattccatg ccggcgaaat gcgcgttcag cgcctcgcac
     8341 agcgcacgtg attcttccgc gcgcagcgca aacgtctcgg ccggcagcac gatgagccgc
     8401 tcgtccatca cccgcaagtg caccgggtcg gcacgcgccc acgagtccgc gccgggatcg
     8461 atgccttgtg caacgagcgt gagcgcccca gccgggtaga cgccttcgcc gagctcgaag
     8521 gcctcctgca cccagcattc cagtgccttc gcctctccgg cgctgcggcg tccccgcgcg
     8581 agcaggagct cgagcgcagc gaagcggctt gtgccgccgc tcgcgaagag acccgggacc
     8641 acgagctcgc agtgcatcag gcccaaagcg gtacgacgcg gacgccgcgc ttgcccaagt
     8701 gccgggagag gatccggtag tggtcgtaat cgaagcgcgg tcgccgcgcc tcgaggagat
     8761 cggcgacctc ggcttccgaa gtcgccgtgc cgaggtagca ccagcggtcg aggatatgca
     8821 cttcggtcgc gtcgcggctt gcgtcggcct cgaccacccc gagcggccca cggtggggcc
     8881 agtccgggct cttcagcggc gctagcgcag cggcgaggcg gccatgatgc gcgtgaatgc
     8941 tctccttgcc cgcgcaaaca cctgcacagc gcccaatttg ataacgaaaa caaggaccgg
     9001 gacgcgcaag tgcgtgttca acccctaaca cttgaagaca aaggcgatgc gcgtcggcgg
     9061 tggcgcgcaa ggcctggagc gctgcgcgcc gcgagcgcca cacgccgtac acgagcggca
     9121 gcgtttcggc gtcgatctcg tcttcgcctg cgagccgcag ccgcttgccg tcgaacgcga
     9181 atccgcacag ccgctcgggg cgccgcagct tgcggttgta ggtcgggtcg agcgccttga
     9241 cgagctgcgc ttcgcgcagc agcgcaccga gctcgcccgc ggtgcgctgc cattcgatgc
     9301 gccgcacgcg cggcgtccag ctcgcgccgg cgatgaaatg ctgcagcacc ctcgagcgca
     9361 gggcgcggct cttgccgatg taaagcggcg ccgcgccctc gccgtagcag aggtaaaccc
     9421 ccggcgcgtc cggaatctcg tcgatcgacg agggatcgag ctgcggcggc aacgtgggcg
     9481 cacgcacgat ctgtcgtgcg gccacgtcga ccacttcgtc gccgtgctcc tccgcggcga
     9541 tgcgcaggaa ttgccagagc gccgctgcat ccggaagcgc gcggtggcgt ggcgcgcagc
     9601 caagcctgtg gcgctcgatc aggctatcga ggtcatggcc gccggtatcc gggtacagcc
     9661 ggcgcgagag ccttacggtg caaagcgtct tcgcgcggta attgatgccg gcgcgctcga
     9721 actcgcgccg caggaagccg tagtcgaagc gtgcgttgtg cgcgacgagc acgcgcccgg
     9781 caagccgctc cgcgagctcg ccggcgatct cggcaaagcg gggcgctgct ccgaccatct
     9841 gcggactgat gcccgtgagc gcctggatca cgccgggaat cggcctgccc ggattgacga
     9901 gcgtgctcca ttgcgcggta agcgcgccgc cctcggcttg cagcacggcg atttcggtga
     9961 tgcggtcgcc ggccggatcg cttccggtgg tttcgacgtc gacgatcgcc agccgcgcct
    10021 gcatggatat acagtatggt aatttgaacc gcgctggagc gcagccaatc gaagaaatca
    10081 tgcgcgcaat cgcacttctc gggctcgtcc tggcggcggg ctgcacgccg atgcagtggc
    10141 agaagccggg catcaccgcg gagcagctcg acgccgacga ggaagactgc cgccagcgcg
    10201 cctggagcga agcgagcctg tacgcctggc agtaccagac catgtggggt cccgtcttcc
    10261 cgcgcgactc ttatgggcgc ggcgcaatgg tctggcccgg gagcccgatg gccgatcctt
    10321 acgggtacca gatgatggag gaaactcggc tcgcccagtt ctgcatggaa gccaagggct
    10381 acaagctggt gccggtgcct aaagagtaac taccccgcag taactatcca cccttcgccg
    10441 gcgggcgcgt ccgctggcaa gccgtagttt gatcgccgcc gggtccagct gcatgcggcc
    10501 tggacggcgc agatcgctgc gtcgagcaga tcgcccgatc cgtcggccac aagcgccctc
    10561 gccagtgcgg gctcgagcgc gacgcggatt ccgagaggct gacccgcctt taacgaccga
    10621 aggatcgctc tccggatcaa cgcccgagcg gccgtatgct cgcttcgcgt gtcgcttttg
    10681 taggaagcgc ggatgccgag ctgcttgcgt atcagcaacc cgggataggc ttccagcgcg
    10741 actcttgacg atcgccgatt ccgcaaagcc ggtatgtgca cgtcgcaggc tgcgagacgg
    10801 cgagcgccct cgtggaacat gagcgccacc ggcggattga cgagcttcat cgggctcgaa
    10861 gagcccgcgg gatggtccgt ggcacgatgg acatatttac tgccggcggg cctcgacgcc
    10921 cgatacacgt caagggtcgc gcgaaaggca agccgatcca tagccgcaca atgctcgacg
    10981 agcccttgcc agtcgtgcgg ccagccgagg tcgcgcacga gctcacgcgg caggctgaac
    11041 ggaaaatcga acccgccgac ccaaggccca ggccgcgcca ggaagcgctc gaaggcgggg
    11101 aagctttcca gcttctccag gccttcgagc ttcagcagct ttttcttgag aatccccgcg
    11161 gcgacagtga tcggcttcgc cctgcgcggc gcgcaggtga agtcgacacc gtagatcctc
    11221 aatcgagcgc tttgcgcaac gcggcgagcg ccgcggattg atgtccgcgc ggccacacga
    11281 gcatcgtgcg cgccttggac acctgctgcg gcagcgcgtg catcgccagc tggttttccg
    11341 cgccgaccac gcgcagcacc gagcgcggaa ccaccgcgat gccgccgccc gcggcgacgc
    11401 acgcgatgat cgcgtggtac gagccgtact ccatgacgcg ctcggcgacg acgctggcgc
    11461 ggccgagcca cgactcgaaa cgccggcgat acgagcatcc ggtggggaac gcgagcacca
    11521 cgcgctgctc gagctctttc gccgtagtga cgcggctgtg gctgcgtggc gtgatgagga
    11581 cgagctcctc cgtgaagacg tgctgcatct cgaggttctt cgtcgcgaac ggctccgcga
    11641 cgaatgccgc ctcgatgtcg ccgcgcagta ccttgtcgac cagcgcgccg ctcgtgccgg
    11701 taacgagctc gagccgcacc ccgggatgcg cgcggtgata ggcggcgagc acgggcggca
    11761 ggcgcgcggc ggcggtgctt tcgagcgagc cgagccggaa cagcccgtgc agcgtatggc
    11821 ccttgaccgc ggcctgggcc tcggtcgaca ggcgcagcag ccgctcggcg tagccgagca
    11881 gcagcctgcc ttccgacgag agcacgagct tgcgcccctg gcggtgaaag agcttcgcgc
    11941 cgaggcgctc ctcgagctgc ttgacgcgcg tggtcacgtt cgattgcacg cggtgcagcg
    12001 cggtggcggc gcgcgtaatg ccgccctgct cggccacggt cttgaagatc tgcagctcga
    12061 ccaggtccat cgttcagaaa tagagaatca tttgttcata attattcatt tctgaagaaa
    12121 catgagtcaa gctatattgt cgctctccgg cgaggtgttc catgcccatt cgcactgcgt
    12181 tgaccgagtc gttaggaatc cgccacccga tcgtgctcgc gcccatggac ctcgtgtccg
    12241 acgcgcgtct gaccgccgcg gtcagcgccg ccggcggcct cggcctgctc ggcggcggtt
    12301 acggcgacga gcgatggctg cgctgcgagc tggacctgct cgagcgctcc aaggcgcgct
    12361 tcggcgtggg gttcatcacg tggagcatgg cgcgccagcc taagctcctc gacctcgcgc
    12421 tcgagcgcaa gccggttgcg gtgatgctgt cgttcggcga cccggccccg ttcgccgacc
    12481 ggatcaagcg cgccggcgcg cgcctcctct gccaggtcca gactctggcc atggcg
//
LOCUS       DASTPD010000320         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_582224, whole genome shotgun sequence.
ACCESSION   DASTPD010000320 DASTPD010000000
VERSION     DASTPD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582224"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFR57_10645"
     CDS             complement(<1..243)
                     /locus_tag="VFR57_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU0259860.1"
                     /translation="MTLYGLARPFLFSLDPEDAHRVALRLAGLVVPRQFQCPVRAMGL
                     EFPNPVGLAAGLDKDAAHIDALARMGFGFLEVGTVTP"
     gene            complement(257..1018)
                     /locus_tag="VFR57_10650"
     CDS             complement(257..1018)
                     /locus_tag="VFR57_10650"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEU0259861.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPVSQFTANRSQRRAWKQHCTLTAHP
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLIEFRDAGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRNASFGTYNVLWQIEQCRALAQPYLYLGYWIRESPK
                     MAYKSKFEPIEGFVGGAWRRLGGRLTQEDRDEKRA"
     gene            complement(1005..1721)
                     /gene="aat"
                     /locus_tag="VFR57_10655"
     CDS             complement(1005..1721)
                     /gene="aat"
                     /locus_tag="VFR57_10655"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU0259862.1"
                     /translation="MLAWLESSTDPFPPVERALKNPNGLLAAGGELSVERLLDAYRRG
                     VFPWYSGEEPVLWWSPDPRMVLYCDELKVPRSLGKSTRNKGYEVRIDTAFAAVLEGCS
                     DRPQTWLGADMKRAYLRLHRAGYAHSFETWLDRELVGGLYGVAIGRMFYGESMFARAT
                     DASKVALVALVQELCGRGFPLIDCQMHTPLLAALGAREISRKSFLRALTTLVNYPAPP
                     GEWSLETKDARDRVSISVKA"
     gene            complement(1715..2404)
                     /locus_tag="VFR57_10660"
     CDS             complement(1715..2404)
                     /locus_tag="VFR57_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU0259863.1"
                     /translation="MERRGFIKFCTASVAALAAPELRADARAQFYSKARLVDEKGATL
                     RASAIPVERNLIFHYPFAATPCFLLNLGKAVKPAQLKTVAGEQYEWKGGVGTARSVVA
                     YSAICAHKLSYPTKEISFISYRTEKSARNRIGNVIHCCSEHSQYDPAQGARVVAGPAP
                     QPLAAILLEWDPANDEIHAVGTLGGEMFKEFFDKFGFRLEMEHGTPRAPVAESCVVQE
                     LDNYCKQQVKC"
BASE COUNT          432 a          756 c          818 g          422 t
ORIGIN      
        1 cggggtaacc gtgcccactt caaggaagcc aaaacccatg cgggcgagcg cgtcgatatg
       61 cgccgcatcc ttgtccaggc ctgcggcgag acccaccggg ttggggaact ccaggcccat
      121 ggcgcggacc ggacactgaa actgccttgg gacgacgagc cccgcgagcc ggagcgcgac
      181 ccggtgggcg tcttcgggat ctagcgagaa aaggaagggt cgagcgagcc catacaaggt
      241 cacatccggt tcccgctcac gcgcgcttct cgtcccggtc ttcttgcgtc agtctgccgc
      301 cgaggcggcg ccaggcaccg cccacgaagc cttcgatcgg ctcgaacttg gacttgtatg
      361 ccatcttcgg gctctcgcgg atccagtagc cgaggtagag atagggctgc gcgagcgccc
      421 ggcactgctc gatctgccac agcacgttgt aggtaccgaa gctcgcgttg cgccggtcgg
      481 gctcgtagaa cgtgtagacc gaggacaggc cgtcgggcag gtagtcgacg atcgagacca
      541 tgacgagctg gccggcatcg cggaactcga tgagccgcgt atcgacgcgg ctctgcagca
      601 agaagtgcga gtattggtcg cggctgtcgt tgtccatgcc gccgcccgaa tggcgcttcg
      661 actgatagcg caggtagagc gcgtaatgct cgagccggaa cttgaggggc tgcgggtggg
      721 cggtaagcgt gcaatgctgc ttccatgcgc ggcgctgcga gcggttcgcg gtgaactgag
      781 agaccggaat gcgcaccgga acgcaggcgc ggcaggcgtc gcaatgcggg cggtacgtga
      841 agatgccgct gcggcggaag ccggccttca ccaggtcgcc gtagagctcg gtgttgatga
      901 gatggcttgg agtagcgacc tgcgagcgcg ccatccggtc cggcaggtag ctgcacggat
      961 acggcgcagt cacatagaac tgcagcacgg catgcggtag gtcgttaagc cttgacactg
     1021 atgcttacgc ggtctcttgc gtccttggtt tcgaggctcc actctccggg aggcgcggga
     1081 tagtttacca acgtggtcaa agcccgcaaa aaagacttcc gggagatctc ccgcgcaccg
     1141 agcgcagcaa gcagcggagt gtgcatttgg cagtcaatga gaggaaatcc acgcccgcag
     1201 agttcctgga ccagcgccac cagggcgacc ttggaggcat cggtggcccg cgcgaacatc
     1261 gattcaccgt agaacatccg cccaatggcc acgccgtaga gcccgccgac cagctcgcgg
     1321 tcgagccagg tttcgaacga gtgcgcgtag ccggcccggt gcagccgcag gtaagctctt
     1381 ttcatgtcgg cgccgagcca ggtctgcggg cggtcggagc agccctctag caccgcggca
     1441 aaggccgtgt cgatgcgcac ctcgtagcct ttgttgcgcg tgctcttgcc gagcgagcgc
     1501 ggcaccttga gctcgtcgca gtagagcacc atgcgcgggt cgggagacca ccacagcacc
     1561 ggctcctcgc ccgagtacca ggggaatacg ccgcggcggt aggcgtcgag cagccgctcg
     1621 accgagagct caccgcccgc cgcaagcagc ccgttaggat ttttcagcgc ccgttcgacc
     1681 ggcgggaagg gatccgtgct gctttcaagc caagctaaca cttcacttgt tgcttgcagt
     1741 agttatcgag ctcctgcacg acgcaacttt cggcgacggg cgcgcgcggc gtgccgtgct
     1801 ccatctcgag cctgaagccg aatttgtcga agaactcctt gaacatctcg ccgccgagcg
     1861 tgccgacggc atgaatttca tcgttggcgg gatcccattc cagcaggatg gccgcgagcg
     1921 gctgcggcgc ggggccggcg accacgcgcg cgccctgcgc cgggtcgtac tggctgtgct
     1981 cggagcagca gtggatcacg tttccgatgc ggttgcgcgc gctcttctcg gttcggtagc
     2041 tgatgaagct gatctctttg gtcgggtatg agagcttgtg cgcgcagatc gcggaatagg
     2101 ccaccaccga acgcgccgtg ccgacgccgc ctttccactc atactgctcg ccggcgaccg
     2161 tcttgagctg cgccggcttg acggccttgc cgaggttgag tagaaagcag ggcgtcgcag
     2221 cgaagggata gtggaagatc aggttgcgct cgaccgggat tgccgacgca cgcaatgtcg
     2281 cgcctttctc gtccacgagc cgcgccttcg agtagaactg ggcgcgcgca tcggcgcgga
     2341 gctcgggggc agcgagcgcc gcgaccgacg cggtgcagaa cttgatgaag ccgcgccgct
     2401 ccattcgtta caggttgtag cctttctc
//
LOCUS       DASTPD010000321         6024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1399839, whole genome shotgun sequence.
ACCESSION   DASTPD010000321 DASTPD010000000
VERSION     DASTPD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399839"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="rodA"
                     /locus_tag="VFR57_10665"
     CDS             <1..831
                     /gene="rodA"
                     /locus_tag="VFR57_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU0259864.1"
                     /translation="FGDVVNGARRWLHVGVTRFQPSEMMKLALPLMLAWYFHRRESTL
                     RLRDFALAALLLAVPLGLIVRQPDLGTAALVGAAGFYVIFLAGLSWKVLGGLAGLFVV
                     ALFPLWGLLHDYQRRRVLTLLDPTQDPLGAGYHIIQSTIAVGSGGISGKGWLNGTQAH
                     LEFIPERHTDFIFAVYSEEFGLIGNLVLLVLYGLLIGRGLVIAANAATLFARLLAGAV
                     SLMFFTYAFVNMGMVSGILPVVGVPLPFLSYGGTALLTLFIGAGILMTVQRHRTLVQR
                     "
     gene            1031..2326
                     /gene="aceA"
                     /locus_tag="VFR57_10670"
     CDS             1031..2326
                     /gene="aceA"
                     /locus_tag="VFR57_10670"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEU0259865.1"
                     /translation="MIEAIRKDWAENPRWRGVKRGYSAEDVVRLRGSVQIEHTLARLG
                     AEKLWKLVNERPYVNSLGALTGNQAVQQVRAGVPAIYLSGWQVAADANDSFQMYPDQS
                     LYAVSSVPTVIRRINNALLRADQIQTMEGNGDIDWLAPIVADAESGFGGVLNAFELMK
                     SMIEAGAAGVHYEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADIMGVPTVVLA
                     RTDAEAADIVTSDIDENDKPFITGERTSEGFYKTRKGFDQALSRGLAYAEYADMVWCE
                     TGTPDLGFAKRFAEGIRRKYPEKMLAYNCSPSFNWKRNLDDATIAKFQRELGAMGYKF
                     QFITLAGFHSLNYSMFELAHGYARSGMSAFVELQQKEFAAVEKGFTAVKHQREVGTGY
                     FDEITQVVTAGKASTTALHGSTEDEQFFDEKKKSKLAVA"
     gene            2341..4113
                     /gene="aceK"
                     /locus_tag="VFR57_10675"
     CDS             2341..4113
                     /gene="aceK"
                     /locus_tag="VFR57_10675"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEU0259866.1"
                     /translation="MRERRSGLPLEIARTILEGFDRHYRLFREASIEARRLFERAAWA
                     EMQAIARERIQMYDRRVEEGVRALLDRYPEAEVDESLWPAIKLAYIGLIHEHRQPECA
                     ETFYNSVACAVLHRRYYNNDFIFWRPAVATEHLEGEVPTYRCFYPLKRGLRSTLREIA
                     TSFELANPWEDLRRDVRHVIRALRNHFPRPTRARPDLQIQVLASLFFRNKGAYIVGRI
                     INAQRELPFAVPILQNELGELYLDTLLLGESRLLVLFSFARAYFFVDMEVPAAYVSFL
                     RWLMPRKPRAEMYMAVGLAKQGKTLFYRDLHYHLKHSSDRFVIAPGIKGLVMLVFTLP
                     SFPYVFKVIRDRFAPPKDLERQTVMDKYLMVKLHDRVGRMADTLEYSLVALPLDRFSP
                     ELLDELRRECASTVQIDGDSLVLGHVYIERRMRPLNIQVEELKRDGDEARLRELLREY
                     GDAIKELAGAGIFPGDMLLKNFGVTRHERVVFYDYDEIQPITDVNFRRIPPPASYDDE
                     IAAEPYWPVGPHDVFPEQFERFLVGDPRARAMFYAVHRDLLDPEFWARKQERVRAGLQ
                     EDVFPYPEEIRFPREGSGPASAGT"
     gene            complement(4067..4297)
                     /locus_tag="VFR57_10680"
     CDS             complement(4067..4297)
                     /locus_tag="VFR57_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU0259867.1"
                     /translation="MIVELHVQPGASRTEFAGKHGERLKVRLQARAVDGKANDALIEF
                     LAAHFGVPRRNVRIVSGLKSRQKRVRILPAGT"
     gene            complement(4294..4845)
                     /locus_tag="VFR57_10685"
     CDS             complement(4294..4845)
                     /locus_tag="VFR57_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU0259868.1"
                     /translation="MFTQIGVFLVDAVASFFVFMLLARFHFQWLRVPFRNPVGEFVLA
                     TTNWIVVPARGAIPGLAGLDLASLFLAWVVQGLGLWLQAAILGAEPGVGMIAAVALVD
                     LVRYSLYILVFAVIVQAVLSWVNPYTPLAPAFDTITRPFLRPLRRYVPPVGNVDLTPL
                     ILLVILYVVLIVVNHLRVAAATV"
     gene            complement(4847..5659)
                     /gene="proC"
                     /locus_tag="VFR57_10690"
     CDS             complement(4847..5659)
                     /gene="proC"
                     /locus_tag="VFR57_10690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU0259869.1"
                     /translation="MRIAFLGGGNMASALIGGLIAKGADARSIAVVEVSAAARERLGA
                     RFPVHIASAPDTALQRCDVLVLAVKPQDAKAAVAPISGTVQGKLLISIAAGVTLETLS
                     RWVGGHRKLVRCMPNTPALIGAGIAGLYALPEVSAQERAAAQSILAAVGEVVWVEEER
                     LLDPVTAVSASGPAYVFWFIEQLAATAEKLGLSRDTSMKLALHTVLGAAKLAASSGDP
                     PATLRRNVTSKGGTTEAALKVFDEEKLAERFVRAVEAASRRATELGAEQGKG"
     gene            complement(5656..>6024)
                     /locus_tag="VFR57_10695"
     CDS             complement(5656..>6024)
                     /locus_tag="VFR57_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU0259870.1"
                     /translation="ARRLSEQRPTHLAPLNVLIQVNVSAEKSKSGIALHEVRDLAEAI
                     IAMPRLRLRGLMAIPEPGAARSRYQEVKRTFDSLKEEFAFDTLSMGMSEDLNIALEEG
                     ATLVRIGTAIFGPRLQKSTV"
BASE COUNT         1073 a         1971 c         1916 g         1064 t
ORIGIN      
        1 ttcggcgacg tggtgaacgg cgcgcggcgc tggttgcacg tgggcgtgac gcgcttccag
       61 ccctcggaga tgatgaagct tgcgctgccg ctcatgctcg cgtggtactt ccatcgccga
      121 gagtcgacgc tgcgtctgcg cgacttcgcc ctagctgccc tgctgctcgc ggtgccttta
      181 ggcctgatcg tgcgtcagcc cgacctcggc accgcggcac ttgtgggcgc cgccggtttc
      241 tatgtcatct tcttggcggg cctcagctgg aaggtgcttg gcgggctcgc cgggcttttc
      301 gttgtcgccc tgttcccgct ctggggcttg ctgcacgact atcaacgcag gcgggtgctt
      361 acgcttctcg atccgaccca ggacccgctc ggagcgggct atcacatcat ccagtccacc
      421 atcgcggtcg gctcgggcgg catctccggc aaaggctggc tgaacggtac tcaggcgcac
      481 ctggagttca tcccggagcg ccacaccgac ttcatcttcg cggtgtattc cgaagagttt
      541 ggactcatcg gcaatctcgt actcctcgtg ctttatggac tgctcatcgg ccgcggcctg
      601 gtgatcgccg cgaacgccgc cacgcttttc gcgcgtcttc ttgccggcgc ggtaagcctg
      661 atgtttttta cctatgcctt cgtcaacatg ggcatggtgt cgggcattct cccggtcgtc
      721 ggggtgccgc tccccttcct ctcgtacggc ggcacggcgc tgcttacact cttcatcggc
      781 gccggcatcc tcatgacggt ccaacgccac cgcacgctcg tgcaaaggta aacgcatcga
      841 gggtgcacgc caggtggcgc gatgcagcaa aatttgaaag agcatgccgg atcatgaaat
      901 gacgctgccc taagtctatg taaatccttg tttttgtaac agagaagtag gccgtatggc
      961 gtgttgctgc gcgccatgcg gcgggggtag cttcctggga ttgccaatca cctgctgagg
     1021 aaccgtaccg atgatcgaag caataagaaa agactgggcc gagaacccgc gttggagggg
     1081 cgtcaagcgc ggctacagcg ctgaggatgt cgttcgcctg cgcggcagcg tacagatcga
     1141 gcacacgctc gcaaggctcg gcgcagagaa gctgtggaag ctcgtgaacg agcggccata
     1201 cgtcaactcg ctcggcgcgc tcacgggcaa ccaggcggtg cagcaggtgc gcgcgggagt
     1261 tccggcgatc tatctctcgg gctggcaggt cgcggcggac gcgaacgact cgttccagat
     1321 gtatccagac cagtcgctgt acgccgtctc ctcggtgccc acggtaatca ggcgcatcaa
     1381 caatgcgctg ctgcgcgcgg accagatcca gaccatggag ggcaacgggg acattgactg
     1441 gctcgcgccg atcgtcgccg acgccgaatc ggggttcggc ggcgtgctca acgccttcga
     1501 gctgatgaag tcgatgattg aagccggcgc cgcgggcgtg cactacgagg accagctcgc
     1561 ttccgtgaag aagtgcggcc acatgggcgg caaggtgttg gtgccgacgc aggaagcggt
     1621 gcagaagttg atcgccgcgc gcctcgccgc cgacatcatg ggcgtgccca ccgtggtcct
     1681 cgcccgcacc gatgccgaag ctgccgacat cgtcacgagc gacatcgacg aaaacgacaa
     1741 gcctttcatc accggcgagc gcacctcgga aggcttctac aagacgcgca aagggttcga
     1801 ccaggccctg tccaggggac tggcgtacgc cgagtacgcg gacatggtgt ggtgcgagac
     1861 cgggacgccg gatctcggct ttgcgaagcg cttcgccgaa gggatacgca ggaagtaccc
     1921 ggagaagatg ctggcgtata actgctcgcc gtctttcaac tggaagcgca acctcgatga
     1981 cgcgacgatc gccaagttcc agcgcgagct gggcgcgatg ggctacaagt tccagttcat
     2041 cacgctcgcc gggttccatt cgctcaacta ctcgatgttc gagctcgcgc acggctatgc
     2101 gaggagcggc atgagcgcct ttgtcgagct gcaacagaag gagttcgcag ccgtcgaaaa
     2161 gggcttcacc gccgtcaagc accagcgcga ggtcggcacc ggctacttcg acgagatcac
     2221 gcaggtcgtg accgcgggca aggcctccac cacggcgctg catggctcga ccgaggacga
     2281 gcaattcttc gacgagaaga aaaaatcgaa gctagcagtc gcctaagccg cgtccgagcg
     2341 ctgcgcgagc ggcgctcggg cctgccgctc gaaatcgcgc gcacgatcct cgagggcttc
     2401 gaccgccact atcgcctgtt ccgcgaggcc tcgatcgagg cgcggcggct gttcgagcgt
     2461 gccgcctggg ccgagatgca ggcgatcgcg cgcgagcgca tccagatgta cgaccgacgg
     2521 gtggaggaag gggtgcgcgc gctcctcgat cgctaccctg aggcggaggt cgacgagtcg
     2581 ctgtggccgg cgatcaagct tgcctacatc gggctgatcc acgagcacag gcagcccgag
     2641 tgcgccgaga cgttctataa ctcggtcgct tgcgcggtgc tgcaccgacg ctactacaac
     2701 aacgacttca tcttctggcg cccggcggtg gccaccgagc accttgaagg ggaagtgccg
     2761 acctaccgct gcttctatcc gctgaagcgt ggcttgcgct cgacgctgcg cgaaatcgcc
     2821 acgagctttg agctcgccaa cccctgggaa gatttgcgca gggatgtccg gcacgtgatc
     2881 cgcgcgctgc gtaatcactt cccgcggccg acgcgagcac ggcccgacct gcagatccaa
     2941 gtgctggcat cgctcttctt tcgcaacaag ggcgcctaca tcgtcggccg gatcatcaac
     3001 gcacagcgcg agctgccgtt cgcggtgccg attctgcaga acgagctcgg ggagctttac
     3061 ctcgacacgc tgctcctcgg cgaaagccgg ctgctcgtgc tgttctcctt cgcgcgcgcc
     3121 tacttcttcg tcgacatgga ggtgccggcg gcgtatgtgt cgttcctgcg ctggctgatg
     3181 ccgaggaagc cgcgcgccga gatgtacatg gcggtcggtc tcgccaagca gggcaagacg
     3241 ctcttctacc gcgacctgca ctaccacctc aagcactcga gcgaccgctt cgtcattgcg
     3301 cccggcatca agggactggt gatgctggtg ttcacgctgc cttcgttccc ctatgtgttc
     3361 aaggtcatcc gcgaccgttt tgcgccgccc aaggacctag agcgccagac ggtgatggac
     3421 aagtacctga tggtgaagct gcacgaccgt gtcgggcgca tggccgatac gctcgagtat
     3481 tcgctggtcg cgcttccgct ggaccggttc agtcctgaat tgctcgatga gctgcgccgc
     3541 gaatgcgcct ccacggtgca gatcgacggc gacagcctcg tccttggtca tgtctacatc
     3601 gagcggcgca tgcgaccgct caacattcag gtggaagagc tcaagcgcga cggcgatgag
     3661 gcgcggttgc gcgagctatt gcgcgaatac ggcgacgcca tcaaggagct cgccggcgcc
     3721 ggaatctttc cgggcgacat gctgctcaag aacttcggcg tcacgcggca cgaacgcgtc
     3781 gtgttctacg actacgacga gatccagccg atcaccgatg tcaacttccg ccgcatccca
     3841 ccgcccgcga gctacgacga cgagatcgcg gccgagccct actggccggt cggcccccac
     3901 gacgtcttcc ccgagcaatt cgagcgcttc ctggtcgggg atccgcgcgc gcgggcgatg
     3961 ttctacgccg tgcaccgcga cctgctcgac ccggaattct gggcgcgcaa gcaggagcgc
     4021 gtgcgcgccg gcctgcagga ggatgtcttc ccctacccgg aggagatcag gttcccgcgg
     4081 gaaggatccg gacccgcttc tgccgggact tgagcccgga aacgatgcgc acgttgcgcc
     4141 gcggcacccc gaaatgcgcg gcaaggaact cgatcagggc gtcgttcgcc tttccatcga
     4201 ccgcgcgcgc ctgaagcctg accttcagcc gctcgccgtg cttgccggcg aactcggtgc
     4261 gcgaggcgcc gggctgcacg tggagctcga cgatcacacc gtggccgcgg cgacgcggag
     4321 gtggttcacc acgatgagaa ctacgtacag gatcacgaga agaataagcg gcgtgagatc
     4381 cacgtttcct accggcggca cgtagcggcg caagggccgc aaaaacggcc gggtgatagt
     4441 gtcgaacgcg ggcgcgagcg gcgtataggg attcacccac gacagcactg cctgcacgat
     4501 tactgcgaac accaggatgt acaggctgta gcgcacaagg tcgacgagcg cgactgcagc
     4561 gatcatgccg actccaggct cggcgccgag gatggccgcc tgcagccaca aacctaggcc
     4621 ctggaccacc caagcgagga acaggctggc gaggtcgagg ccggcaagtc ccggaatcgc
     4681 gcccctcgcc ggcacgacaa tccagttggt ggtcgccagc acgaactcgc cgaccggatt
     4741 gcgaaatggc acccgcagcc actggaaatg aaaccgcgca agcagcatga agacgaagaa
     4801 gctcgccacc gcgtccacca agaagacgcc gatctgggtg aacatgtcag cctttcccct
     4861 gctccgcgcc gagctcggtg gcgcggcggc ttgccgcttc aactgcgcgc acgaaccgct
     4921 cggcaagctt ctcctcgtcg aagaccttga gtgccgcttc ggtggtgccg cctttggagg
     4981 tcacattcct gcgcagagtg gcgggcgggt cgccgctcga ggcggcaagc ttggccgcgc
     5041 caagcacggt gtgcagcgca agtttcatgg aagtgtccct gctcaagccc agcttctctg
     5101 ccgtcgcggc gagctgctcg atgaaccaga acacgtaagc cggcccgctc gccgagaccg
     5161 cggtcacggg atcgagcagc cgttcctctt ccacccacac cacttcgccg actgcggcca
     5221 gtatgctctg cgccgcggcc ctttcctgag cgctcacctc cggcagcgca tagaggccag
     5281 cgattcccgc tccgatcagc gcgggggtat tcggcatgca gcgcacgagc ttgcggtggc
     5341 cgccgaccca gcgcgacagc gtttccagcg taacacccgc cgcgatgctg atgagaagct
     5401 tgccttgaac agttccggaa atcggcgcta cggcggcttt cgcgtcctga ggcttgaccg
     5461 cgagcaccag cacgtcgcag cgctgcagcg cggtatccgg cgcggacgca atgtgaaccg
     5521 ggaagcgcgc gccgaggcgt tcgcgcgccg ccgcgctaac ctccacgact gcgatcgagc
     5581 gcgcgtcggc gcctttggcg atcaggccgc cgatcagcgc gctcgccatg ttcccgccgc
     5641 cgaggaatgc gatcctcaca ccgtgctttt ctgcagccgg ggaccgaaga tcgcggtccc
     5701 gatccgcacc agggttgcgc cttcctcgag cgcgatgttg aggtcctcgg acatgcccat
     5761 cgaaagcgta tcgaacgcga attcctcctt cagggaatcg aacgtcctct tcacttcctg
     5821 gtagcgcgag cgggccgcgc cgggttcggg gatcgccatc aacccgcgca ggcgcagtcg
     5881 cggcatcgct atgatggctt ctgcgagatc cctcacttcg tgaagtgcaa tgccgctctt
     5941 gctcttctct gccgacacgt tgacctgtat cagcacgttg agcggcgcaa gatgcgtcgg
     6001 gcgctgctcc gagaggcgcc gcgc
//
LOCUS       DASTPD010000322         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_788944, whole genome shotgun sequence.
ACCESSION   DASTPD010000322 DASTPD010000000
VERSION     DASTPD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788944"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFR57_10700"
     CDS             complement(<1..470)
                     /locus_tag="VFR57_10700"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259871.1"
                     /translation="MLEHAPMLRSRSFEETRAYLKSSHDIDMDLVGRPGYDTSVDVRL
                     NALFLPAQWVAYLQYGAAARLRLSSFSSPWLAREPGRNQLPAVYWVHFSLRGSLKTVI
                     RGQAFEGDKHRGIVLSPVGDKVLHTSADNSRLSLAMREDALNRHLSALLGYSPR"
     gene            630..1868
                     /locus_tag="VFR57_10705"
     CDS             630..1868
                     /locus_tag="VFR57_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259872.1"
                     /translation="MQAGQAQMRAGEWVLVAAAALLLAITMGSRSVFGLFISPLNSAT
                     GLGIATISFAVAVSQLTWGAAQPFAGMLAERYGAARMIVAGSLLAAAAGAFVPSAGSA
                     GLLVAALAVSGIAATVGSPSLLVGTVSQRVSAERRGLVAGLVGAGGPLGQLLLAPATQ
                     AVISAAGWVAAMLSVAALALLSIPLALAFRGGPAPAVADAHSAQQAGAGLREALSSRG
                     FWLINATFFVCGVHVFFLITHLPGVVEVCGLPPSVSGAALAVLGLFNIAGSIGAGWVI
                     QHYSMKATLSWLFAARAAGIALFLVAPKTEFTVLAFCVWMGLTYMAVLPPTAGLIGQL
                     YGAKRLPTLLGITFMLHQVGAFLGSWLGGVFVEATGSYDGMWVLDLGLGVAAAVLALT
                     IRERAAGRVLTRAALARTLA"
     gene            complement(1833..2921)
                     /locus_tag="VFR57_10710"
     CDS             complement(1833..2921)
                     /locus_tag="VFR57_10710"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU0259873.1"
                     /translation="MLETTIAGSLPKPGWLAETGKLWPTWRAQGAELERAKRDATLLW
                     LKEQEDAGVDIVTDGEQSRQHFVHGFLEFVEGIDFEHKVKMGIRNNRYDAMVPVVVGP
                     LRLKGRVHQSEAVLLRKHSKHKIKLTLPGPMTIVDTIADRHYGDKLKLAMAFAGLLNQ
                     EARALEKDGVDVLQFDEPAFNVYMDDVKRWGIDALHRAIEGLKCKTCVHICYGYGIQA
                     NVDWKKTLGESWRQYEKIFPALAKSRIDQVSLECIHSKVPIELLGLLNGKDVLVGVID
                     VAADAVETPQEVSEVIGKALKYVPARHLFPCTNCGMAPMDREVAVQKLHALAAGAALA
                     RKRLAAGERTVGWGSSRLGKSTRKRRSR"
BASE COUNT          444 a         1020 c         1057 g          581 t
ORIGIN      
        1 tcccgcggcg aatagccgag cagcgcggag agatgccggt tgagtgcgtc ctcgcgcatg
       61 gcgagcgaaa gccgggaatt atcggcggaa gtgtgcagga ccttatcccc gacaggagag
      121 agcacgatcc cgcgatgctt gtctccctcg aatgcctgcc cgcgaatgac cgtcttcagg
      181 ctccctcgga gcgagaagtg aacccagtaa acggcgggca gctggttgcg cccgggctcg
      241 cgggcgagcc acgggctcga gaaggacgat aggcgcaagc gggcggccgc gccgtactgg
      301 aggtacgcca cccactgggc gggcagaaaa agcgcgttca gccgcacgtc cacggaggtg
      361 tcgtacccag gccgcccgac gagatccatg tcgatgtcgt ggctgctctt gaggtaggcg
      421 cgcgtctcct cgaagctgcg tgagcgcagc atcggcgcgt gttcgagcat ttaacaaaga
      481 tagcagccgg tccgtttccc gctggtttcg gccgtgtttc cggaacgtaa cagcggccga
      541 tacgtgctat ttccggatag aaacaagccg gtaaccgagc gcaaagcggc agacatcggg
      601 ccgatacggc gtgcgcgcag tatgccgcca tgcaagccgg tcaggcgcag atgcgtgcgg
      661 gcgagtgggt tctcgtggcg gcggccgcgc tgctgcttgc catcaccatg ggctcccgct
      721 cggtgttcgg gctgttcatc tcgccgctca actcggccac gggcctgggc atcgctacga
      781 tcagctttgc ggtcgcggtg agccagctca cctggggcgc tgcgcagccc tttgccggca
      841 tgctggccga gcgctacggg gcagcgcgca tgatcgtggc cggaagcctc ctcgccgccg
      901 cggcgggcgc atttgtgccc tccgctggca gcgcgggtct gctcgtcgcc gcgcttgcgg
      961 taagcggcat cgccgccacc gtcggcagcc cgtcgcttct cgtcggcacg gtgagccagc
     1021 gcgtttccgc cgagcggcgc ggtctcgtcg ccgggctcgt gggcgctggc gggccgctgg
     1081 ggcagctcct gctcgctcct gccacgcagg cggtaatctc ggctgccggc tgggtcgccg
     1141 cgatgttgtc ggtcgccgcg ctggcgctcc tctccatccc gctcgcgctc gccttccggg
     1201 gcgggccagc gccggctgtc gcggatgcgc actcggcaca gcaggccggc gccggcctgc
     1261 gtgaggcgct ctcgagccgc ggcttctggc tcatcaacgc cacgttcttc gtgtgcggcg
     1321 ttcacgtgtt ctttctcatc acgcacctgc ccggcgtggt cgaggtgtgc ggcctgccgc
     1381 ccagcgtttc gggcgccgcg cttgccgtct tgggcctctt caacatcgcg ggcagcatcg
     1441 gagccggctg ggtcatccag cactactcga tgaaggccac gctgtcgtgg ctgttcgccg
     1501 cgcgtgcggc aggcatcgcg ctcttcctgg tcgcgccgaa gaccgagttc accgtgctcg
     1561 cgttctgcgt gtggatgggg ctgacttaca tggcggtgct gccgcccacg gcgggcctca
     1621 tcggacagct ttacggcgcg aagcggctgc ccacgctgct cggcatcact ttcatgctcc
     1681 accaggtggg cgcgttcctc ggctcctggc tgggcggcgt gttcgtcgag gcgaccggca
     1741 gctacgacgg catgtgggtg ctcgacctcg ggctgggcgt ggcagccgcg gtgctcgccc
     1801 tgacgatccg cgagcgggct gcagggcggg ttctaacgcg agcggcgctt gcgcgtactc
     1861 ttgcctaggc ggctggagcc ccagccgaca gtacgctcgc ccgctgccag gcgcttgcgc
     1921 gcgagcgcgg cgccggcggc gagggcgtga agcttctgca ccgcgacctc gcggtccatc
     1981 ggcgccatgc cgcagttggt gcaggggaag aggtgacgcg cggggacgta cttgagcgcc
     2041 tttccgatca cctcggagac ttcctgcggc gtctcgaccg catcggcggc gacgtcgatc
     2101 acgccgacga ggacatcctt gccgtttagg agcccaagca gctcgatcgg aaccttcgag
     2161 tggatgcact cgagcgagac ctggtcgatc ctgctcttcg cgagcgcggg gaagatcttc
     2221 tcgtactgcc gccacgattc gccgagcgtc tttttccagt cgacgttcgc ctgaatgccg
     2281 tagccgtagc agatgtgcac gcaggtcttg cacttgagac cttcgatggc gcggtgcagg
     2341 gcgtcgatcc cccagcgctt gacgtcgtcc atgtacacgt tgaacgccgg ctcgtcgaac
     2401 tgcagcacat ctacgccgtc tttctcgagc gcgcgcgctt cctggttgag caggcccgcg
     2461 aaggccatgg cgagcttcag cttgtccccg tagtgccggt cggcgatggt gtcgacgata
     2521 gtcatgggac cggggagcgt cagcttgatc ttgtgtttgg aatgctttcg aagaagaaca
     2581 gcttcgctct ggtgcacgcg gcccttgagg cgcagcggcc ccacgaccac cggcaccatg
     2641 gcgtcgtagc ggttgttgcg gatgcccatc ttcaccttgt gctcgaagtc gataccctcg
     2701 acgaactcga ggaagccgtg cacgaagtgc tggcgcgact gctcgccgtc ggtgacgata
     2761 tcgacgcccg cgtcttcctg ctccttgagc cagagcagtg tggcgtcgcg cttcgcgcgc
     2821 tcgagctcag cgccttgcgc gcgccacgtc ggccacagct ttccggtttc agcgagccag
     2881 cccggcttgg gaaggcttcc ggcgatggtg gtttcaagca tccttacgcc tcactataat
     2941 cgagcccaac aagggaggga aacgcgtgct gggcatcttg gtggggacgt tttgctcgtt
     3001 catcgcaggt atcttcaacc cgtacgtcgc caacaaagcc ggccccgact ggatgtgggg
     3061 cggcggcagg gacgacctgc tgcgaaagct ctacttccgt cc
//
LOCUS       DASTPD010000323         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_381966, whole genome shotgun sequence.
ACCESSION   DASTPD010000323 DASTPD010000000
VERSION     DASTPD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381966"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1308)
                     /locus_tag="VFR57_10715"
     CDS             complement(1..1308)
                     /locus_tag="VFR57_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0259874.1"
                     /translation="MTKGKKDHRTTHVRPDDIDPKHRWDRPLQAPGRTQVDFEERVDF
                     RRLHDYRLARVRAALAHSGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGE
                     PYIWDFGSAARHHKLYSPWLREDHCRAGLLGMRGATGADLTLFKDAAREIKAILEEEG
                     VADMPLGVDVVEPPMLFALQKAGIEVRDGQQVMLQAREVKNIDELTLLNMAAAMVDGV
                     YQDIAEALKPGVQESQIVALATKRLYEMGSDCVEAINSISGERCSPHPHNFTDRLIRP
                     GDQAFFDIIQSFMGYRTCYYRTFNVGRATPAQRTAYKKAREWMDRAIELLRPGMTSDK
                     IALALPEAKEIGFASEMDAFGLNFCHGLGLGLHERPLISRLTSLREPIELKAGMVFAV
                     ETYCPATDGVSAARIEEEVILAPKGAKIITLYPAEELPIANPY"
     gene            complement(1411..1812)
                     /locus_tag="VFR57_10720"
     CDS             complement(1411..1812)
                     /locus_tag="VFR57_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0259875.1"
                     /translation="MPLIIDASVALAWFVRNQATGHTDRVRRQARSERLHVPAVWPLE
                     FANALWQLQRRKLLSDRQVDTIIDLAEPLDIVVHKDTPAPRRLVAVARDHDLTAYDAS
                     YLDLALALRYPVACRDGPLRNALHAAGLKLA"
     gene            complement(1812..2060)
                     /locus_tag="VFR57_10725"
     CDS             complement(1812..2060)
                     /locus_tag="VFR57_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU0259876.1"
                     /translation="MDKLSVFEAKARFSEVVSRAEEGKQTVITKRGKAVARVVPEKAA
                     RAERWDRSGAIDRIVEFSKTCRTRRKVDLQRLIEEGRL"
BASE COUNT          385 a          775 c          746 g          437 t
ORIGIN      
        1 tcagtagggg ttggcaatgg gcaattcctc ggcgggataa agggtgatga tcttggcgcc
       61 tttcggggca aggattactt cctcttcgat ccgcgccgcg gacacaccgt ccgtcgcggg
      121 gcaataggtt tccacggcga ataccatgcc cgccttgagc tcgatcggct cgcgcagcga
      181 cgtgagccgc gagatgagcg gacgctcgtg caatccgagg ccgaggccgt ggcagaagtt
      241 gaggccgaac gcgtccatct cgctcgcgaa gccgatctcc ttcgcctccg gcagcgcgag
      301 cgcgatcttg tcgcttgtca tgccgggccg gagcagctcg atcgcccggt ccatccactc
      361 gcgcgccttc ttgtaggcgg tgcgctgcgc gggcgtggcg cggccgacgt tgaaagtccg
      421 gtagtagcag gtgcgataac ccatgaacga ctggatgatg tcgaagaacg cctggtcgcc
      481 ggggcggatc aaacgatcgg tgaagttgtg cgggtgaggg ctgcagcgct cgcccgagat
      541 cgagttgatc gcctccacgc agtcggagcc catctcgtag agccgcttgg tggcgagcgc
      601 aacgatctgc gactcctgca ccccgggctt caacgcttcg gcgatatcct ggtatacgcc
      661 gtcgaccatc gccgccgcca tgttgaggag cgtgagctcg tcgatgttct tcacttcgcg
      721 cgcctgcagc atcacctgct ggccgtcgcg cacctcgatg ccggccttct gcagcgcgaa
      781 cagcatcggc ggctcgacca catccacgcc gagcggcatg tcggccacgc cttcttcttc
      841 gaggatggct ttgatctccc tggcggcgtc cttgaacaat gtcagatccg cgcccgttgc
      901 cccgcgcatg ccgagcagtc cggcgcggca atggtcctcg cgcagccacg gcgagtagag
      961 cttgtgatgg cgcgcggcgc tgccgaaatc ccagatgtag ggctcgccgt tgccagtgag
     1021 gagcgagtag cgcgtgagct tgtcgcgcgc ccactcgccg atcacggtgc tcgtggtgta
     1081 gcgaatgttg tgctggtcga acgaaaggag cgcgccaagc ccggaatgcg cgagtgcggc
     1141 gcgcacgcgc gcgaggcggt agtcgtgcag acggcggaaa tcgacgcgct cctcgaaatc
     1201 gacctgcgtg cgcccgggcg cctgcagcgg ccggtcccag cggtgcttcg gatcgatgtc
     1261 gtcgggacgg acgtgcgtcg tcctgtggtc tttctttcct ttcgtcatcc ggcctcctcg
     1321 cgcggctgat tctagcgtct aattttcttc acaggccgat gcagccgctg cgcaatcgct
     1381 gtcgtagccg ttttgctttc ggagacccaa ctaagccaac ttcaagccgg ccgcgtgaag
     1441 cgcattcctg agcgggccgt cccggcacgc caccgggtaa cgcagtgcca gcgcaagatc
     1501 gagatacgaa gcgtcgtaag cagtgaggtc gtgatcccgt gcaaccgcca ccaggcggcg
     1561 cggcgccggc gtgtctttgt gcacaacgat gtccagaggc tcggccagat cgatgatcgt
     1621 gtcgacctgg cgatccgaca gcagcttgcg gcgttgcagc tgccagagag cattcgcgaa
     1681 ctcgagcggc cagaccgccg gcacgtgcag ccgctcgctg cgtgcctgcc gcctgacgcg
     1741 atcggtatgg ccggtcgcct gattgcgtac gaaccaggcg agcgccacgc tcgcgtcgat
     1801 gatcagcggc actacagccg gccttcctcg atcaacctct gcaggtcgac tttgcgccgg
     1861 gtacggcaag tcttgctgaa ctcgacgatc cggtcgatcg ccccggagcg gtcccatcgc
     1921 tccgcgcgcg cagccttctc cggcacgacg cgcgcgaccg ctttgccgcg cttggtgatc
     1981 accgtctgct ttccttcttc cgctcggctc accacctcgg aaaaccgcgc tttcgcttcg
     2041 aacacgctca gcttgtccat gtcttctgca cctggttaac cagacggggt attctagccg
     2101 aacagtgccg agggacaatc cggcgatccc cagataggcg agccgccgac aaatactgaa
     2161 actcgttcag catgcgcgcc ggcagcggaa gcgcggcaac tcttatcgcg atgtccggca
     2221 gaagtctaga attccctacg tgcgtgaaac ccagattccc gccgcgcggc tgaccgaatt
     2281 cgccagcgcc tgcctggcgc ggctcgacct tgcgccggcc gacgcgcgcc tggtcgccga
     2341 gac
//
LOCUS       DASTPD010000324         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1812721, whole genome shotgun sequence.
ACCESSION   DASTPD010000324 DASTPD010000000
VERSION     DASTPD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812721"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..506
                     /locus_tag="VFR57_10730"
     CDS             264..506
                     /locus_tag="VFR57_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259877.1"
                     /translation="MLFIAGLGGGFLVFVHQALGWFKSSIWTSVSVVTGLQWLGVRWA
                     TGPQEWRGLHEVLGNVPLAIALPVIGVLAALFARPR"
     gene            954..1607
                     /locus_tag="VFR57_10735"
     CDS             954..1607
                     /locus_tag="VFR57_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0259878.1"
                     /translation="MAKQRISYFPVEKMDAEMQKEMDRCRREGTPRPESSAVRAHVPA
                     AFWAFANSWRDLFRNGGCEHTIKELCRLYVSRSVQCDYCGNQRSMKAAAAGMMEDDVR
                     DLLNFEKSARYSDKQKAALAYAEAITWHLDTDDAFWQRLYRHFSEPELVELGCFIALT
                     MGQQSWLRLLNIEHHQVLAGTAASLAPGYEDPESLHRNKSSPDYWAKTRAHNGGARS"
     gene            1579..2640
                     /locus_tag="VFR57_10740"
     CDS             1579..2640
                     /locus_tag="VFR57_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0259879.1"
                     /translation="MRITAALVPEKGAPFQLEALELADPGPDELVVRVAACGVCQTDV
                     HGRDDYFGIPFPCVFGHEGAGTVERAGAAVTKVRPGERVVMTSPACGNCAACRRGLPG
                     YCAEARRIKFGGRLRDGRIPLKKNGSPVSGGFFQQSAFATHALATEGNVVRLPDDLPL
                     EVAAAFACGVNTGAGAVLNVLKPQPGSSVAVFGAGSVGLAAVMAARISRCAAICAVDL
                     HDNRLELARELGATHVSREKPPRSFDFTLEAAGSPQALRDAVDCLAPLGTCCLVGSAR
                     KGAEARLEMTQLQHGRTVRGCIQGDAPPGEFFPRLFELWRAGRLPVERLIAYYGLAEV
                     NRAVADSLSGKTVKPVLRI"
     gene            2669..3631
                     /locus_tag="VFR57_10745"
     CDS             2669..3631
                     /locus_tag="VFR57_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259880.1"
                     /translation="MKIACKLFALLLAAFAVPTHAQNYPTKPVRLIIAFTPGSSTDIV
                     GRAVAAKLQELWGQPVVAENRTGAGGSIGSAAVLREAPDGYTLLANSSAHVANPSIYK
                     TLPYDTLKDFANIAPLAGGPNVLIVSPQTGWKSFADFVAAAKAKPGQLNFSSAGVGSG
                     THFNLEKLKLMAGIDVAHVPYKGTPEAIGDTVAGRVCCYWAPINAALPHVRGGKALAL
                     GVSSAQRSSLLPDVRTIAEQGVPGFDYTLWVGLWGHAALPADIVTKINADVNRALASP
                     DLAQRLTNLGTIPMKMSSAEFAQFVRKEVEDTAKVLKAAGIQPQ"
BASE COUNT          625 a         1180 c         1188 g          645 t
ORIGIN      
        1 tggtttagtg cagaactgct ttgccttgca atccccgatg cagagactgc gcttcgcgcg
       61 actcagtgtc aacgcaatag cagcagaagc gcgcacagga atcaaacatc actgcctatg
      121 cctttacgcc ctccggaaga tagcgatcac aatcaccgct tgagctcgcc aggcgcgccg
      181 tctggcgcgg cggaagttag actaaaaacc agagtccaga gacgatgctc aaagaacgcc
      241 gcgggtccag cgcacttgct gccgtgttgt tcatcgcagg ccttggtggc ggtttcctcg
      301 tgttcgtaca tcaggcgctc ggctggttca agagctcgat atggacttcg gtcagcgtgg
      361 tgaccgggct gcagtggcta ggcgtgcgat gggcgacagg gccgcaggag tggcgggggt
      421 tgcatgaggt gctcgggaat gtgccgctcg cgatcgcact gccggtaatc ggtgtgctgg
      481 ctgctctctt cgcaaggcct aggtaaagcc gctcttcctc ggctcgtact cgtggtcggc
      541 ttgttaaccg gcttctagcc ggggctgtgt acgtcgtgta agtgttcgcg attgtttctc
      601 aggaagttcg ccacggcctt cttcagatga tatgtgtaag cgcccgattt cttggaccaa
      661 taagtttctt cttgccggcc ctgcccgctt ctgcgcgtat cgtgccgctt gcctgccttg
      721 ctgaaattct cgtccatagg gatctctgtc gcgacagtac gaacgctacg ccttccccga
      781 ggtagcgaca ttgatccatg tcagacggca gccgcttacc gggcgcgcga agcacgctcg
      841 ccgtgttcgt cgatcccggc gggccagcgc aggccgcaag ggctggtccg atcgatgact
      901 tagcctccgg ttccgcctct tcggatgata gatttacgct agaggaggac gccatggcca
      961 agcagcgtat cagttacttt ccggtcgaga agatggacgc cgagatgcag aaggagatgg
     1021 atcggtgccg ccgcgaaggc acgccgcgcc ccgagagctc ggcggtgcgc gcgcacgtgc
     1081 cggcggcgtt ctgggcgttt gccaattcgt ggcgcgacct cttcaggaac ggggggtgcg
     1141 agcacaccat caaggagctt tgccggctgt acgtgtcgcg ctcggtgcag tgcgactact
     1201 gcggcaacca gcgctcgatg aaggccgcgg ccgccgggat gatggaggac gacgtcaggg
     1261 atctgctcaa cttcgaaaaa tcggcgcgct actcggataa gcaaaaagcc gcgctcgcct
     1321 acgccgaagc gatcacctgg catctcgaca ccgacgacgc gttctggcag cggctctacc
     1381 ggcacttcag cgagcccgag ctggtcgagc tcggctgctt catcgcgctc accatgggcc
     1441 agcagagctg gttgcgcctg ctcaacatcg agcatcacca ggttctcgcc ggcaccgcgg
     1501 cgtcgctggc gccgggatac gaggacccgg agtcgctgca caggaacaaa tcttcaccgg
     1561 actactgggc gaaaacgcgt gcgcataacg gcggcgctcg ttcctgagaa gggcgcgccg
     1621 ttccagcttg aagcgctcga gctagccgat cccggacccg acgagctcgt ggtgcgcgtc
     1681 gccgcctgcg gcgtgtgcca gaccgacgtc cacggccgcg acgactattt cggcatcccg
     1741 ttcccctgcg tcttcggcca cgagggcgcc ggcacggtag agcgcgccgg cgccgcggta
     1801 accaaggtgc ggccagggga gcgcgtcgtc atgacgtcgc cagcgtgcgg aaactgcgcc
     1861 gcgtgccgca gggggctacc cggctattgc gccgaggcgc ggcgcatcaa gttcggcggg
     1921 cggctgcgtg acggaagaat tcctctcaag aagaacggtt caccggtttc aggcggcttc
     1981 ttccaacagt ccgctttcgc cacgcacgcg ctcgccacgg aaggcaacgt ggtgaggctg
     2041 ccagacgacc tgccgctcga agtggcggcc gcattcgcat gcggcgtcaa taccggcgcg
     2101 ggtgcagtgc tcaatgtgct taaaccgcag cccggcagct cggtcgcggt gtttggcgcg
     2161 ggttcggtcg gccttgccgc ggtgatggcc gcgcgcatct caaggtgcgc cgcgatctgc
     2221 gcggtggatt tgcacgacaa ccggctcgag ctcgcacgcg agctcggcgc cacgcatgtc
     2281 tccagggaaa agccgccgcg gtcgttcgac ttcacgctcg aggcggcggg tagcccgcag
     2341 gcgttgcgcg acgcggtgga ttgcctcgcg cctctcggga cctgctgcct ggtgggaagc
     2401 gctcgcaagg gcgccgaggc gcggctcgag atgacccagc ttcagcacgg aagaaccgtg
     2461 cgcggctgca tccagggaga cgctccgccg ggcgaattct tcccgagact cttcgagctt
     2521 tggcgcgccg gcaggctgcc ggtcgaaagg ctgatcgcct actacggtct tgccgaggtc
     2581 aaccgcgcgg tcgccgattc gttgagcgga aagacagtga agcccgtgct tcggatatga
     2641 tgatttttca aacctcatgg aggaggggat gaaaatcgct tgcaagctct tcgcgctgct
     2701 tctcgccgcg ttcgccgtgc cgacgcacgc gcaaaactac ccgaccaagc cggtacgcct
     2761 catcatcgct ttcacgcccg gcagctccac cgacatcgtt ggccgcgcgg tggccgcgaa
     2821 gctccaggag ctgtgggggc agccggtggt tgccgagaac cgcaccggcg cgggcggctc
     2881 gatcgggtcc gcggcggtgc tgcgcgaggc gcccgacggc tacacgctcc tcgccaattc
     2941 gtccgcgcac gtcgccaacc cgtccatcta caagaccctt ccctacgaca cgctcaagga
     3001 cttcgccaac atcgcgccgc tcgccggcgg cccgaacgtc cttatcgtct cgccgcagac
     3061 cggatggaag tcgttcgcgg atttcgtcgc cgccgcgaag gcgaagcccg gccagctcaa
     3121 cttctcctcc gccggcgtgg gcagcggcac gcacttcaac ctcgagaagc tcaagctcat
     3181 ggcgggcatc gatgtggcgc acgtgccgta caaaggcacg ccggaagcga tcggcgacac
     3241 cgtcgccggc cgggtgtgct gctactgggc gccgatcaac gccgcgctgc cccacgtgcg
     3301 cggcggcaag gcgctcgcgc tcggcgtgtc gagcgcgcag cgctcgagcc tcttgcccga
     3361 cgtgcggacg atcgccgagc aaggcgtgcc gggattcgac tacacgctct gggtcgggct
     3421 gtggggacac gcggcgctgc cggccgacat cgtcaccaag atcaatgccg acgtgaatcg
     3481 cgcgctcgcc agccccgacc ttgcgcagcg gctgaccaat ctcggcacca tcccgatgaa
     3541 gatgtcgagc gccgaattcg cgcagttcgt gcgcaaggaa gtcgaggaca ccgccaaggt
     3601 ccttaaggcc gccggcatcc agccgcaatg aagaatct
//
LOCUS       DASTPD010000325         7783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_792656, whole genome shotgun sequence.
ACCESSION   DASTPD010000325 DASTPD010000000
VERSION     DASTPD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792656"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1318
                     /locus_tag="VFR57_10750"
     CDS             203..1318
                     /locus_tag="VFR57_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0259881.1"
                     /translation="MKSLGARSQGLRLERMKASPRWAGEGFHNVHPILPGLRNPNVPM
                     PTLTEFLCGGERRVPARPLPSVSPLEEWRRRPGSGLRATWLGHSTVLIEIDGVRVLTD
                     PVWGPRASPLLLAGPKRFQPVPVPLSAMPRVDVVIISHDHYDHLDYPTIRALAKANVP
                     FVASLGVGAHLEAWGVARERIVELDWWESHALPKGGLTVSAAPSQHFSGRTPRSRNAT
                     LWSSFVIRGERHAVFFSGDTGLTAEYQAIRERMGPFDLVMLEVGGLHPSWGDMHLGPE
                     NALKALALLGGGAFLPVHWGTFSLAMHAWDQPAEALLELAPKADARLVMPRLGQPVEP
                     DRADRIEPWWREVDTLVEPARADTTIKLPTTMPFPID"
     gene            complement(1329..2564)
                     /locus_tag="VFR57_10755"
     CDS             complement(1329..2564)
                     /locus_tag="VFR57_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0259882.1"
                     /translation="MGVIALTLALAYGIWYSYSVILVALLAEFGWSRSVLAGAFSVFT
                     LVHGGVNPLIGALCARFRPLRVIAVGGAAMGATLFLDSFISSPLGLYLAFGVATAIAV
                     ALAGWVPSLVFVQRNFQERLGLSVGIISSGVGVGMLLIVPLTQLLIDAFGWRVAFQVL
                     GLIAVVWIVPSSLWLRHLMLSQIPENRGQTPARNRGQTPTGRSGSDPTLRQAMRTEPF
                     WLLMAMFFFGNVASQTLHVHQVAYLVDQGLAAIAAASVVGVVGLASIVGKTGGGWLSD
                     RVEREVVYVAGIAILVASAFVLLSLKTGSSAWAAYGYAVLLGIGYSVTAAITPVIVSD
                     RFSGPHFGAIVGVGLMGTAAGSAIGPWMAGRLYDLTASYAIPFMIAAACGALAGAAAW
                     RARTLRKIGVRALKSADAG"
     gene            complement(2665..3216)
                     /locus_tag="VFR57_10760"
     CDS             complement(2665..3216)
                     /locus_tag="VFR57_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259883.1"
                     /translation="MRVLISACLVLAGCVGPPSAPEMLTPAARAQSELQTRRYDGARP
                     EDVLPAAVAALQDFGFQVVKSDTAVGVIVAQRGYKRTFGEYHYEFWQGYWQTMRNTFT
                     FQWHSRGPNPEKMFGPAGVSAAVSITPAATGSEVRVSLHRFVSRPTGEPILVWAEELA
                     EPEVYAKFFDLLAQALAREAPRK"
     gene            complement(3216..3647)
                     /locus_tag="VFR57_10765"
     CDS             complement(3216..3647)
                     /locus_tag="VFR57_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259884.1"
                     /translation="MTRALAVLLVAFLAACAKPGPGDVLAGGESMLKLRSAQTRTFET
                     TDRNRVMRGVVATLQDLGFMVTGADAALGTVSGRKFTADATGQVYELRVTVTVRPRDE
                     RQMLVRANAEFNNKPVEDPAAYQNFFNALGRSIFLVANKAD"
     gene            complement(3656..4201)
                     /locus_tag="VFR57_10770"
     CDS             complement(3656..4201)
                     /locus_tag="VFR57_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259885.1"
                     /translation="MRRNSERALLVAALAFAGCAEMPPEYIAVKPGDAAQRELQGRRF
                     DGIGEKELLDAAVGVLQDLGFTVDITSAELGFVQGAKEREAKAPDQTLVILIVAALAA
                     SQGSTLPAGQMRQDQTISVLLTVRQAREGDMGSHIVLVTFHNHVRQPLMRTAGPLRDP
                     VLYQSFFELLSKALFLQAHKL"
     gene            complement(4185..4622)
                     /locus_tag="VFR57_10775"
     CDS             complement(4185..4622)
                     /locus_tag="VFR57_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259886.1"
                     /translation="MKTQMAALAVAILLSGCMSAGEHRDAVQGEGERLTVGKVQREIR
                     VGMPSAEVARVLGSPNIVTTDEERREVWVYDRISTETVYSESRGGVAALVLATSGSAA
                     GAAAPVYSAGAGARSTTQRTLTVIVKYDKESRVRDFSYHASQF"
     gene            4790..5563
                     /locus_tag="VFR57_10780"
     CDS             4790..5563
                     /locus_tag="VFR57_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0259887.1"
                     /translation="MNTRLASAIERRYGSSLRLSADLPGLDALAALNERSVCRRYRPD
                     AVPEDVVRLLCATALAAPTKSDLQQATILRLADPAKRAAVCALLPGSPWLARAPELFI
                     FCADGWRLRRLFAKRGTQFPNEHLDAFFNAAVDAAIALGAFVSAAELAGLGTCPISQV
                     RDHVARIDELLALPDWVVPVAGLALGYPEAKEPLSARLALSATVHTDRYDTAAVEREL
                     DAYDSRRGRDWSGDKVRQYTTVWRSDFGAHVRRKKYRLE"
     gene            complement(5607..5879)
                     /locus_tag="VFR57_10785"
     CDS             complement(5607..5879)
                     /locus_tag="VFR57_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0259888.1"
                     /translation="MTFILWLVIGGILGWLASIVMKTNDQQGIILNVIVGIVGAFLGG
                     LLLAPLFGTGTINQSDFSIGSLFVSFLGAIILLAIVNFFRGRRART"
     gene            complement(6213..6551)
                     /locus_tag="VFR57_10790"
     CDS             complement(6213..6551)
                     /locus_tag="VFR57_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0259889.1"
                     /translation="MAPGLAWQYVERYFVWSPQPVDQALLRRAREIELRYKLSWWDSM
                     IVAAAQLQECALLLTEDLQDGAVFGAATARSPFTPEVREAAARYTVERQGAPLHRPRG
                     RPRRGAAPVA"
     gene            complement(6567..6818)
                     /locus_tag="VFR57_10795"
     CDS             complement(6567..6818)
                     /locus_tag="VFR57_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU0259890.1"
                     /translation="MKKLTVRLDEKTAARARVKAAERSMSLSRYIGEVLRKELRHDDA
                     YAAAYRAWRQDRKPWPLKGPPVRHPKREELYDRPVFRRR"
     gene            complement(6839..7162)
                     /locus_tag="VFR57_10800"
     CDS             complement(6839..7162)
                     /locus_tag="VFR57_10800"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU0259891.1"
                     /translation="MRVLLDECVPRALREQLPGHEVRTVAESEWAGVTNGDLLRLAAE
                     RFDVLLTVDRNLEYQQNFLGAAVAVIVMHAPSNDIAALQPLMRKVLEAIPEAKRGKVT
                     NIRSS"
     gene            complement(7146..7367)
                     /locus_tag="VFR57_10805"
     CDS             complement(7146..7367)
                     /locus_tag="VFR57_10805"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0259892.1"
                     /translation="MERSSVVHSDPEILGGTPVFVGTRVPLQALIDYIEGGHTLEKFL
                     DDFPSVPRELAIAALEQAKRHLIADARAA"
     gene            7525..>7783
                     /locus_tag="VFR57_10810"
     CDS             7525..>7783
                     /locus_tag="VFR57_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259893.1"
                     /translation="MTLVDLSPLALAYCIAVLLLNFTLRGSLGFGGAPGLPLLALALP
                     VKVIAPAWSLVGIVSSIAIVGHGRRHVDWREFKSLLPGCMLG"
BASE COUNT         1298 a         2729 c         2469 g         1287 t
ORIGIN      
        1 tcactaatac cccgacggct cgcctcggcg atagcgtgcg gcgtaacaat cacccgaatg
       61 gtcccctccc gaggaaggga acgttcagta gctggcgcgg ttgacgccag tattcgggct
      121 ctgaccccga ttttccggcg ccgatttccg gcgcccgatt tccgctccgg ggtctgaccc
      181 cgatttcaag tatggtggtg ccgtgaaatc tctcggtgcc agatcgcagg gcctgcgcct
      241 cgagcgcatg aaggcgtcgc cgcggtgggc gggcgagggc ttccacaacg tgcaccccat
      301 cctgccggga ctgcgcaacc cgaatgtgcc catgccgacg ctgacggagt tcctgtgcgg
      361 cggcgaacga cgcgtgccgg cgcgcccttt gccttcggtg agtccgctcg aagaatggcg
      421 ccgcaggccg ggcagcggct tgcgcgcgac ctggctcggc cattcgacgg tgctcatcga
      481 gatcgacggc gttcgtgtcc tcaccgaccc ggtgtgggga ccgcgcgctt ccccgttgct
      541 gctcgccggt ccgaagcgct ttcagccggt gccggtcccc ctgagcgcca tgccgcgggt
      601 cgacgtagtg atcatctcgc acgatcacta cgaccacctc gattacccca ccatccgcgc
      661 gctcgccaag gcaaacgtgc cgtttgtcgc ttcgctcggc gtgggcgcgc acctcgaagc
      721 ctggggtgta gcacgcgagc gcatcgtcga gctcgactgg tgggaatcgc atgctctgcc
      781 gaagggcggg ctcacggtga gcgcagcgcc atcgcagcac ttctccggcc gcaccccgcg
      841 gagccggaac gcgacgctct ggtcatcgtt cgtgatccgc ggcgagcgtc acgccgtctt
      901 cttcagcggc gataccggcc ttaccgccga atatcaggcg atccgcgagc gcatggggcc
      961 gttcgacctc gtgatgctcg aagtgggcgg gctgcatcct tcgtggggcg acatgcatct
     1021 gggacccgag aacgcgctca aggcgcttgc acttctcggc ggcggggctt tcctgcccgt
     1081 gcactggggg accttcagcc tcgcgatgca cgcgtgggat cagccggcgg aagcgctcct
     1141 cgagctcgca ccaaaggcgg acgcaaggct cgtcatgccg cggctcggtc agccggtcga
     1201 gccggaccgc gctgaccgga tagagccctg gtggcgcgag gtagatacgc tcgtcgaacc
     1261 ggcgcgcgca gacacgacga ttaagcttcc caccacgatg cctttcccga tcgactaagg
     1321 tttcacgtct agcctgcgtc ggcagatttc agggctctga ccccgatttt ccgcagcgtc
     1381 cgcgcccgcc aggccgccgc tcccgcaagc gctccgcacg ccgccgcgat catgaaagga
     1441 atcgcgtagc tggcggtcag gtcgtacagc cgcccggcca tccacggccc gatcgcgctc
     1501 cccgccgcgg tgcccatgag gcccacgcct acaatcgcgc cgaagtgcgg cccgctgaag
     1561 cgatcgctca cgatgacggg cgtgatcgcc gccgtgaccg agtagccgat gccgaggagc
     1621 accgcgtagc cgtacgccgc ccacgcagaa gatcctgttt tcagggaaag caatacgaac
     1681 gcgctcgcga cgagaatcgc gatgcccgcg acgtacacca cctcgcgctc cacgcggtcg
     1741 gagagccacc ctccgcccgt cttccccacg atgctcgcga gccccaccac gcccaccacc
     1801 gacgccgccg cgatcgcggc aagtccctga tcgacgaggt acgccacctg gtgcacgtgc
     1861 agcgtctgcg acgccacgtt gccgaagaag aacatcgcca tcagcagcca gaacggctcc
     1921 gtgcgcatcg cttgtctcag ggtggggtca gaccctgatc ttcctgttgg ggtctgaccc
     1981 cgatttcttg ccggggtctg accccgattt tccggaatct gactcagcat caagtgccgc
     2041 agccacagcg aagacggcac gatccacacc accgctatca accccagcac ctgaaaagcc
     2101 acccgccacc cgaacgcatc gatgagaagc tgcgtgagcg gcacgatgag cagcatgccg
     2161 acgccgacgc ccgagctgat gatgccgacc gagagcccga gccgctcctg gaagttcctc
     2221 tgcacgaaga cgagcgaggg cacccagccg gcgagcgcga ccgcgatcgc ggtcgcgacg
     2281 ccgaaggcga ggtagaggcc gagcggcgag gagatgaagc tgtcgaggaa gagcgtggcg
     2341 cccatcgccg ccccgcccac ggcgatcacg cgcagcggcc tgaagcgcgc gcagagcgcg
     2401 ccgatgagcg ggttcactcc cccgtgcacc agcgtgaaca ccgagaacgc gcccgcgagc
     2461 accgagcggc tccaaccgaa ctcggcgagc agcgccacca ggatcacgct gtacgagtac
     2521 cagatgccgt aggcgagcgc gagcgtgagc gcgattacgc ccaaggcgat agagcggctg
     2581 gaaatcgggg tcagagcccg aattatcgcc gctggggtca gaccctgatt tttcctgaga
     2641 gggtctgacc ccgatttccg acctttactt tcgcggcgcc tcgcgcgcga gcgcctgggc
     2701 gagcaggtcg aagaacttgg cgtacacctc gggctcggcg agctcttcgg cccacacgag
     2761 gatcggctcg ccggtcggcc ggctgacgaa gcggtgcaag ctcacgcgca cctcgcttcc
     2821 ggtggccgct ggcgtaatcg acaccgcagc gctcacgccc gccggcccga acatcttttc
     2881 cggattcggc ccccggctgt gccactggaa ggtgaaggtg ttgcgcatcg tctgccagta
     2941 gccctgccag aattcgtagt ggtattcacc gaaggtccgc ttgtaaccgc gctgcgcgac
     3001 gatcactccg accgccgtgt cggacttcac cacctggaag ccgaagtcct gcagtgcggc
     3061 gaccgcggcg ggcagcacat cttccggccg cgcgccgtcg tagcggcgcg tctggagctc
     3121 ggactgagcg cgcgcggccg gcgtcagcat ctcgggagcc gagggtgggc cgacgcaccc
     3181 cgccagcacg aggcacgccg aaatgagcac acgcatcagt ccgccttgtt ggcgacgagg
     3241 aagatcgagc gcccgagcgc gttgaagaag ttctggtacg ccgccgggtc ttcgaccggc
     3301 ttgttgttga actcggcgtt cgcgcggacg agcatctggc gctcgtcgcg cggccgcacc
     3361 gtaacggtca cgcgcagctc gtacacctgg ccggtagcat cggcggtgaa cttgcgcccg
     3421 ctcaccgtgc cgagcgcggc gtcggcgccg gtcaccatga agccgagatc ctgcagcgtc
     3481 gcgaccactc cgcgcatcac ccggttgcgg tccgtggtct cgaaggtgcg cgtctgcgcc
     3541 gagcgcagct tcagcatgct ctcgccgccc gcgagcacgt cgccggggcc cggcttcgcg
     3601 catgccgcga ggaaggcaac aagaagcacc gccagggcac gcgtcatagt cgcagtcata
     3661 acttgtgtgc ctgaaggaag agcgccttgg agaggagctc gaagaacgac tggtagagca
     3721 ccgggtcgcg gagcgggccc gcggtgcgca tgagcggctg gcgcacgtga ttgtggaatg
     3781 tcaccaggac gatgtggctg cccatgtcgc cctcgcgcgc ctggcgcacg gtgagaagca
     3841 cgctgatcgt ctggtcctgg cgcatctgcc cggcggggag agtgctgccc tggctcgccg
     3901 caagcgcggc cacgatcagg atgacgagcg tctgatcggg agccttcgcc tcgcgctcct
     3961 tggccccctg gacgaagccg agctcggcgc tcgtgatgtc gaccgtgaag cccaagtcct
     4021 gcagcacgcc gaccgcggcg tcgagcaact ccttctcgcc gataccgtcg aagcgccgcc
     4081 cctgcagctc gcgctgggcc gcgtctcccg gcttgactgc aatgtattcg ggcggcattt
     4141 ccgcgcagcc cgcaaatgcc aacgcggcga cgagcagcgc cctctcagaa ttgcgacgca
     4201 tggtaggaga aatcgcgcac gcggctctcc ttgtcgtact tcacgatcac ggtcagcgta
     4261 cgctgcgtgg tcgagcgcgc gcccgcgccg gccgaataga ccggtgctgc ggcgccggcg
     4321 gcgcttccgc tggttgcgag cacgagggcg gcgacgccgc cgcggctctc ggagtacacc
     4381 gtctcggtcg agatgcggtc gtaaacccac acctcgcgcc gctcctcgtc ggtggtgacg
     4441 atattcggcg agccgagcac gcgtgcgacc tccgcgctcg gcatcccgac cctgatctcc
     4501 cgctgcacct tgccgacggt aaggcgctcg ccttcgccct gcaccgcatc gcggtgctcg
     4561 cccgcggaca tgcagcctga gaggaggatc gcaaccgcga gcgctgccat ttgcgttttc
     4621 atggacggtt ctccgcgaat tccacagtag gcgaccccgg cgggcgctat cttgatctag
     4681 cgcaaaagcg cagcctccct ctgcaaagct gccacttctg cctttgggcc ggtatcccct
     4741 gatcgtgggg gtcagacccc gaatttgttg ctgccttagg attgggcgca tgaatacccg
     4801 cctcgcttcg gccatcgaac gccgctacgg ctcctcattg cgcctgtccg ccgaccttcc
     4861 tggcctcgac gcactcgccg cgctcaacga gcgctcggtg tgccggcgct atcgcccgga
     4921 tgcggtcccc gaggatgtgg tgcgccttct atgcgccacc gcactcgccg cgcccaccaa
     4981 gagcgacctg cagcaggcga cgatcctgcg tctcgccgac cccgcgaagc gcgcggcggt
     5041 atgcgcgctc cttcccggct cgccgtggct cgcgcgcgcg ccggagttat tcatcttctg
     5101 cgccgacggc tggcgcctgc ggcggctctt cgcgaagcgc ggcacgcaat tcccgaacga
     5161 gcacctcgac gccttcttca acgccgcggt cgatgccgcg atcgcgctcg gcgctttcgt
     5221 ctccgccgca gaactcgccg gcctcggcac ctgccccatc agccaagtgc gcgaccacgt
     5281 ggcgcgcatc gacgagctcc tcgcgctccc cgactgggtc gtgccggtcg ccggcctcgc
     5341 gctcggctac ccggaagcaa aggaacctct gagcgcccgc ctcgcgctct ccgccaccgt
     5401 gcacaccgac cgctacgaca ccgccgcggt cgagcgtgag ctcgatgcct acgactcgcg
     5461 ccgcggccgc gactggagcg gcgacaaagt gcgccagtac accaccgtgt ggcgctccga
     5521 ctttggcgcg cacgtcaggc gcaagaaata ccgcttggaa tagaggtcag accccgagtt
     5581 tggtcttggg gtctgacccc gagttcttaa gtccgcgccc gcctgccacg gaagaagttg
     5641 acgatggcga gcaggatgat cgcgcccagg aacgacacga agagcgaccc gatgctgaag
     5701 tcgctctggt tgatcgtgcc ggtgccgaag agaggcgcga gcaagaggcc gccgaggaac
     5761 gcgccgacaa ttcccacgat cacgttcagg atgatcccct gctggtcatt cgtcttcatg
     5821 acgatgctgg caagccagcc cagaatgccg ccgataacga gccacagaat aaaggtcata
     5881 cgccctcctg taggtagggt tcgcccgggt ggcgggatgc cggccggccg atggccctcg
     5941 attgaccgca actgtcgtgc cgcgatcccg gaagaggggt cgtcgggaga atgattttca
     6001 tgacaacccg aaacgacccc cggtaggcgc tggaaaccat ccggaaacct cgcggcaacg
     6061 ggaaagacaa actgcgccgg ctcacgcgcc ggatactgct cgcacgatgc gacaccatgc
     6121 aagagctgtc tgcctcgccg cggcagtagt caccaccttc ctcgcggtgg tcgactcgaa
     6181 catccgcgcg aacgaggtcg ttctggcaga cattacgcga ccggcgccgc cccccgcctg
     6241 ggcctgccgc ggggccgatg cagaggggcg ccttgccgct cgaccgtgta cctggccgcg
     6301 gcttctctca cctccggcgt gaacgggctg cgcgcggtcg cggcgccgaa gacggcaccg
     6361 tcctgaaggt cttcggtgag gagcagcgcg cactcctgaa gctgcgcagc agcgacgatc
     6421 atgctgtccc accacgagag cttgtagcga agctcgattt cgcgcgcgcg gcgcagcagc
     6481 gcctggtcca cgggttgagg actccaaacg aaataccgct cgacgtactg ccaggcgagt
     6541 ccgggtgcca tcccaaactt tcggcgttag cgtcgacgaa acacgggccg gtcatacagc
     6601 tcctcgcgct tcgggtggcg caccggcggc cccttcagag gccagggctt cctgtcctgg
     6661 cgccaggccc ggtacgccgc cgcgtacgcg tcgtcgtggc gcagctcctt gcgcagcacc
     6721 tcgccgatgt agcgcgacaa gctcatgctg cgctccgccg ccttgacgcg cgcccgcgcc
     6781 gcggttttct cgtcgagcct gactgtcagc ttcttcatct gaggtcagac ccgcaatctc
     6841 acgagctgcg gatgttggtt accttgccgc gtttagcttc aggtatcgcc tccaacacct
     6901 tgcgcatcaa cggttgtagc gccgcgatgt cattgctcgg tgcgtgcatc actatcaccg
     6961 cgacggcggc accgaggaaa ttctgctggt actcaagatt gcggtcgact gtgaggagga
     7021 catcgaaacg ctcggcagcc aggcgcagga gatctccgtt cgtcacgccc gcccattcgc
     7081 tctctgcgac ggttctgact tcgtggcctg gaagctgttc gcgcagcgct cgtggaacgc
     7141 actcgtcaag cagcacgcgc atcggcgatc aggtgccgct tcgcctgctc gagcgctgcg
     7201 atggcgagtt ccctcgggac tgaggggaag tcgtcgagaa acttctccag cgtgtggccg
     7261 ccttcgatat agtcgataag cgcctgaagc ggcacgcggg taccgacgaa cacgggcgta
     7321 ccgccgagga tctcgggatc ggaatgaacg accgaagaac gttccacagg cgacagtcta
     7381 ctcccatgag caggaggaca tctgtccacg attcggccgt cggtttggcg tgtttactct
     7441 ggtagtccac gtacgcatgc atgcaccgat cgtcgtgcgt tctcaaaata ggcaacgcgc
     7501 gagtcgaaat agcgtgcacc cgacatgacc ctggtcgacc tctcccccct cgccctcgcc
     7561 tactgcatcg cagtcctgct cctcaacttc accctgcgcg gcagcctcgg cttcggcggc
     7621 gcgcccggac tcccgctcct cgcgctcgcg cttcctgtga aggtgatcgc gcccgcgtgg
     7681 agcctcgtcg gcatcgtctc ctccattgcg atcgtcggcc acggccgccg gcacgtcgac
     7741 tggcgcgagt tcaagagcct gctgcccggc tgcatgctcg gca
//
LOCUS       DASTPD010000326        14383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1201656, whole genome shotgun sequence.
ACCESSION   DASTPD010000326 DASTPD010000000
VERSION     DASTPD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201656"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFR57_10815"
     CDS             complement(<1..287)
                     /locus_tag="VFR57_10815"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0259894.1"
                     /translation="MTGATVLDPGTASLILFGVFFALLVLRVPVAFALGLACLPVFLI
                     EDRFTPQNLVQETFNAYNSFILLAVPFFLLTANLMNVGGITTRLVRLSRDLV"
     gene            complement(284..805)
                     /locus_tag="VFR57_10820"
     CDS             complement(284..805)
                     /locus_tag="VFR57_10820"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0259895.1"
                     /translation="MLRKITHWYSTLLSWLLAASVAILVFPVSLQIFSRYTELIPSYI
                     WTEEMARFFFIWSIMLGAMVGIREGTHFVVDLWPPLNARAQAAIRLVASVFVLVFACV
                     FLWWGIDFTRFALNRISELAELPLWYIHLPWPLAGVTWILFLGEQMWDDLRIVSGKAP
                     PKSGPETNLESAT"
     gene            complement(851..1825)
                     /locus_tag="VFR57_10825"
     CDS             complement(851..1825)
                     /locus_tag="VFR57_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0259896.1"
                     /translation="MKRIVLAAIAAAFAVPAQAQLTLHGASQFNDDHAFTKAMVKFEE
                     LVKKYSGGKAVNFVLHKNSELGLEKQYFEYMAQGKAVDYAIVSPAHMSTFSRAAPFID
                     APFLFRDLKHWNAVLDQDVLKPVADEIAQKADVMLIGYAGGGTRNIFVNKPVKNMQEL
                     KGLKVRVQGAPIWSRTFQAAGMAPTVIAYNEVYNAIQNGVIAAGENEAAGVEQMKFYE
                     VAPHLSMTQHAITIRPLCFSGKTFKKLDKQLQDAVLKAGKEAGAYGRQIESSEDEEKL
                     VKMEKEGKLKRVQFQGRDQMKKLVDPVMAAYAKEIGAEQILAKINSIK"
     gene            complement(1822..2643)
                     /locus_tag="VFR57_10830"
     CDS             complement(1822..2643)
                     /locus_tag="VFR57_10830"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0259897.1"
                     /translation="MRLRKLGSFGAEAETIDPDFGKVWNAFFASQVLVFRGQKFTPSG
                     FLAFARQFGQPEPHVIDQFHHPEHADILILSNVQQDGKPIGLADAGTYFHTDYSYLDV
                     PARATTLYSIQVPKKGGDTLFADQYAAYDGLPSGMKKRIEGLVALHHYGNRDDQDKRS
                     RTVASVLTEEQEQKMNWVRHKVARKHPVTGRTALYAVSGSSFRIEGMPDDEALDLLDE
                     LKGHATQEKYQLRLKYGVGDVVIWDNASLLHSATLTDPSDPRTLWRITIKEEKPT"
     gene            complement(2634..3779)
                     /locus_tag="VFR57_10835"
     CDS             complement(2634..3779)
                     /locus_tag="VFR57_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0259898.1"
                     /translation="MKRDTVLTIRLPSGDYAVREPGHFEKPKAPFTRRALAAAHVVAD
                     PLSTKEPWLEAAIDWEATLAYRRHLWSWGFGVAEAMDTAQRGMGLDWKNSLELIRRTV
                     AEGGGIVASGAGTDHLPAGVHSLADIIAAYEEQCAAIEKLGGRIILMASRALAASAKS
                     PEDYGKVYERILSQVREPVIIHWLGEMFDPALEGYWGQRDHYAAMEVCLSILEKHARK
                     VDGIKISLLDKGKEVAMRRKLPKGVRMYTGDDFNFAELIEGDERGYSDALLGIFDAIA
                     PAASQALAFLAQMRMAEYHEVLAPTVPLSRHIFGAPTRFYKTGVVFMAWLNGHQDHFV
                     MVGGQQSARNIQHLSELFRLADAAGLLMDPPLACARMKQLLAVHGCG"
     gene            complement(3776..4921)
                     /locus_tag="VFR57_10840"
     CDS             complement(3776..4921)
                     /locus_tag="VFR57_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0259899.1"
                     /translation="MSERRIGIIMHGVTGRMGMNQHLIRSIIEIRKQGGVALKNGDRV
                     MPDPVLVGRNAAKVEELARRHHVQRWSTDLDKALASADEIFFDAASTQLRPKLLKKAI
                     AAGKHIYCEKPVSDTLAEAKDLYAVAKKARVKHGVVQDKLWLPGLLKLRKLRDEGFFG
                     RILSVRGEFGYWVFEGDRVPAQRPSWNYRKEDGGGIILDMLCHWRYVLDNLFGEVQAV
                     SCLGATHIPYRWDEKGKKYKATADDAAYATFLLKGNVVAHFNSSWDVRVRRDDLLTLQ
                     VDGTQGSAVAGLRKCLVQSYDKTPKPVWNPDIPQPINFYEGWEEVPDENYDNAFKVQW
                     ELFLKHVAGEGPFRWNLLEGAKGVQLAELGLKSWKQRKWLDVPRLKP"
     gene            complement(4918..5367)
                     /locus_tag="VFR57_10845"
     CDS             complement(4918..5367)
                     /locus_tag="VFR57_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259900.1"
                     /translation="MSFRVLGTNHTSFTVSNLDRTVAFFRDCLGFELVSRAPRDPALV
                     SRITGVENADVVIAFLKAPGHTLELIEYRSPATKASVKARPCDTGFAHVAFNVDDAAA
                     AAEAVQRYGVKPIAPPVAIDKGPNKGRKVVYARDADGVTLEFIEVGK"
     gene            complement(5364..6692)
                     /locus_tag="VFR57_10850"
     CDS             complement(5364..6692)
                     /locus_tag="VFR57_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0259901.1"
                     /translation="MRFSVKEISFFERDVRLRMPFRFGIVTLTEAPQAFALARIELEN
                     GKQSQGAAAELLAPKWFDKNLALSNEDNFEQLRASMRSARNAVLDAGMNTAFGHSRPT
                     LGLVENYGPALLDRALLDALCRALGISFYQAVKTNVAGIAFRDLDMQGFLSGLEPRSE
                     IAARHTVGLVDPITSADNKKPVNDGLPETLEEVVARYGHRWFKLKVGGDVKADVERLR
                     AIAAVLDRIAEPYHASLDGNEQYEDVEGVIALWNAIETEPRLKRLASSIVFVEQPIKR
                     QKALAANIAALSARKPVIIDESDDSLDALPRARALGYRGVSSKTCKGLYKSLLNAARC
                     AAWGKDYFMTGEDLTIQPGLALQQDLALVSILGLTHVERNGHHYVNGMAGLPAQEQQD
                     FGSAHPDLYERSHGAVRLRIHKGIIQMKTLDCPGYASRAMPDWQSMRRMA"
     gene            complement(6689..7522)
                     /locus_tag="VFR57_10855"
     CDS             complement(6689..7522)
                     /locus_tag="VFR57_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0259902.1"
                     /translation="MRIALCNEVIAPMPFPRQCEYAAKLGYDGLEIAPYTLSDEPHRL
                     GSAQIAAARVAAEDAGVAVTGLHWLLLKPAGLSISSKDEKVRKRTIDVMLAFIDQCAE
                     LGGRYLVHGSPHQRRVDPGETRAAAMARAQESFASIAERAQKAGVVYCIEPLSSEQTP
                     LINTLDEASRMVEEIGSPAVRSMLDCSSAGRMEKEPLNALVERWLPKGVIAHVQVNDR
                     NRRGPGQGEQRFAPLFASLKRHGYRGDVAVEPFDYVPDGPGAAARAIGYIKGIMEALE
                     T"
     gene            complement(7519..8994)
                     /locus_tag="VFR57_10860"
     CDS             complement(7519..8994)
                     /locus_tag="VFR57_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0259903.1"
                     /translation="MEGLSALGRSIGNFAAFWPIVYTLGATLVGILIGCMPGLSATLG
                     IALMTTLTIKMAPNDAILILICVYVGAIYGGSRTAILLNIPGTAANAAACLDGFALAQ
                     KGQAGRAMGIATTGSVVGSLFGVICLALFTPALGEVALSFGAFEFFWLGVFGVMMTGS
                     LTGDDALKGWIMGFLGLFAAQIGQEGIHAFNRFTFGSGDLAGGISLIPALIGAFGFAE
                     ILTVLTAPQAKPIVNSVDSVLPRIHDVLRYWKTVLRSGVIGVYVGILPGVGEDMAAWS
                     SYAAAKRASAEREQFGKGSVEGLIAAETGDNASIPGGIIPALALAIPGSAPAAVLMAA
                     MIIHGVQPGPMLMVSQPQFVYDVVTMTLFATLAILFFGLFMVRPLLKIIQVPRSIIMP
                     IIFVLCTVGSFSIASRLFDVYIMLAIGVAGFVLRKLGYPMAPFVLGLVLGDIVDKSLR
                     RGLVLSDGSLAPFFTRPISAVIFAIVLFTIFMVMRKAWRRT"
     gene            complement(8995..9453)
                     /locus_tag="VFR57_10865"
     CDS             complement(8995..9453)
                     /locus_tag="VFR57_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0259904.1"
                     /translation="MKSDRLQGAFWIALGLAIFYASWTMDRLANLGVKPFSAPGLLPG
                     ILGAFIVLLGIAMLLRARQEEAEAIEWRRILLPLALCLGFAAGMVGRGIPFWLAAWIF
                     IAVMIWTLQYRERRAKGELPRLAAISVSIGLAASVAISLIFQELFLIRLP"
     gene            complement(9450..10445)
                     /locus_tag="VFR57_10870"
     CDS             complement(9450..10445)
                     /locus_tag="VFR57_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259905.1"
                     /translation="MNRTVVVCAAALLAASAQAQNWKPAKPVTIIVPWAAGGSTDQVT
                     RITAAELEKHLGQKVVIVNQPGASGSIGTKSALDAPKDGHTWTAGAAQDLGVYRALGM
                     LETNLTDWHLFLSVANVPLVGVNPNTPYKTMQDLLDAMKAKPGEVTVATAGVTSGGHN
                     AMEAIARATGVKYRHVTYDGGNPAVVATVAGETQVTTQLAVEQAEMIRGKRLRPLAAV
                     ADKPLEIEGHGRIDPITQTLPGMKIPTNYFGVFVPKGVPPQVVATLDRVWGDHITRSE
                     ALKKYATSRGALFAPMSGQDAQKAALPAVQANAWLLHAGGKTKVSPDTVGIPKPE"
     gene            complement(10448..11437)
                     /locus_tag="VFR57_10875"
     CDS             complement(10448..11437)
                     /locus_tag="VFR57_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259906.1"
                     /translation="MSRFLTLFAGALLALPLQAQNWKPTKPITIIVPWAAGGATDQVT
                     RLAAAELEPALGQKIVVVNQPGGSGSIGTRNAMEAPKDGHTWTAGAAKQLGTYPLLGM
                     LNSKVDDFHLYLSVTNVSIVSVNPGTPYQSLQQLLDDMKARPGQVKVATAGNTSSGHF
                     AMEAIAKAAGVKYRHVTYDGGNPAVVATVAGETELTTQLAVEQAEMIKGKRLRPLAVV
                     ADQPLTIEGVGTIAPITQTLPGFPKVTTAFGIFIPKGVPAEVAATMDRIWTERVAKSE
                     KIRQYAQSRGALFTPAVGKAAHDLVWPTVVEDAYMLQEAGMVKVSPESIGIRR"
     gene            complement(11439..12041)
                     /locus_tag="VFR57_10880"
     CDS             complement(11439..12041)
                     /locus_tag="VFR57_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0259907.1"
                     /translation="MNHTQQKILEAATAEFARHGLGGARVDRIAARAGANKRMLYYYF
                     GDKEGLFLAALEDRYAHIRNAERSLELEHLDPRIAVRRLVEFTWNYFLEHPEFMTLLN
                     SENLHKGRHVRRSRRVPEMHSHLVGLLRRVLRRGEAQRLFRKGVDPVQLYISIAGEGY
                     FYLSNRYTLSRIFGRDLMAPRALAGRARHISDMILNALRR"
     gene            complement(12038..12790)
                     /locus_tag="VFR57_10885"
     CDS             complement(12038..12790)
                     /locus_tag="VFR57_10885"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259908.1"
                     /translation="MREPFIHLSGVRKNYGEHLAISDATFDVLPGELVSLVGPSGCGK
                     STLLKILAGLHPHDGGEVRIGSPTHPFDPAKDVGMVFQAPLLLKWRRVLENVLLPAEI
                     LGLPGKEARERAGHLLKLVGLAGAEHKYPYELSGGMQQRAAIARALVHDPKLVLMDEP
                     FGALDALTREKMNLELLRIWKEAGKTIVFVTHGISEAVFLGTRVVVLTAGPARMADNF
                     EVELPYPRTLDMKTNERFGEYTRRVYRLLGME"
     gene            complement(12783..13541)
                     /locus_tag="VFR57_10890"
     CDS             complement(12783..13541)
                     /locus_tag="VFR57_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0259909.1"
                     /translation="MNSKVGRWTLAVLAHLVLLAAWYLFVRFGEVPKFVMPSPGATLD
                     ALLQPNYDWWTNIAVTGTEIFVGYLIALVVGVALALVFTWSKTLEAFFMPLLVSLNMI
                     PKVALGPLIIVWFKYGVFPNSIMAFSIAVFPILLTTARGLREIEPELLDLVRSLKGSR
                     WQLFVKIQLPGALPYIFSGMKVGAILAVAGAIVGEFLGSDKGLGYLMLQVQVTLDTPA
                     MFMAVLLITLLGMLLYGLVLILERILVVKDARVA"
     gene            complement(13538..>14383)
                     /locus_tag="VFR57_10895"
     CDS             complement(13538..>14383)
                     /locus_tag="VFR57_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0259910.1"
                     /translation="AGLYEPVLRIALEETKTWAKRPVVLIAGLAGKTSAAKREAQLAR
                     SLGYHAGMLSLGAMKGASIDALIRHCSAVAEEIPLVGFYLQPAVGGIALPMEFWRRFA
                     TIENVAAIKIAPFNRYATLDVIRGVVAARAEERIALYTGNDDHIVLDLAVPFDVPRDG
                     ERVRVRIAGGLLGHWSVWTKTAVQLFDRTKSERLSEDLLALDARVTDCNSAFFDVAHN
                     FAGCIPGCHEVLRRQGLLAGTWCLDPKEKLSPGQAAEIDRVYREHADLSDDAFVGANL
                     ERWLE"
BASE COUNT         2516 a         4716 c         4619 g         2532 t
ORIGIN      
        1 accaggtctc gcgagaggcg cacgagccgg gtcgtaatcc cgccgacgtt catcagattg
       61 gcggtcagca ggaagaacgg caccgcgagg aggatgaacg agttgtaggc gttgaaggtt
      121 tcctgcacca ggttctgcgg cgtgaagcgg tcttcgatga ggaagaccgg caggcacgcc
      181 aggccgagcg cgaaagccac cggcacgcgc agcaccagca gcgcgaagaa gactccgaag
      241 aggatgaggc tcgccgtgcc cgggtcgagg acggtcgcgc cggtcatgtc gcgctttcga
      301 ggttcgtttc cggcccggat ttaggcggcg ctttgccgga gacaatgcgc aggtcgtccc
      361 acatctgttc accgaggaac aggatccagg tcacgccggc gagcggccac ggtagatgga
      421 tgtaccagag cggcagctcg gcgagttcgg agatgcggtt caacgcgaag cgcgtgaagt
      481 cgatgcccca ccaaaggaac acgcacgcga agacgagcac gaacacgctc gcgacgaggc
      541 ggatcgccgc ctgggcgcgc gcgttgagcg gcggccagag gtcgacgacg aaatgggtgc
      601 cctcgcggat gccaaccatg gcgccgagca tgatcgacca gatgaagaag aaccgcgcca
      661 tctcctcggt ccagatgtag ctcggaatga gctccgtgta gcgcgagaag atctgcagcg
      721 acaccgggaa gacgagaatc gccaccgagg cggcgagcag ccaggacagc agcgtgctat
      781 accagtgcgt tatttttctc agcacaaaaa aacggcggca cgcgccgcgc cgccgtttcc
      841 tcccccgggg ttacttgatg ctgttgatct tggccaggat ctgctcggcg ccgatctctt
      901 tcgcgtacgc cgccatcacc gggtcgacca gcttcttcat ctggtcgcgg ccctggaact
      961 gcacgcgctt gagcttgccc tccttctcca tcttcacgag cttctcctca tcctcggaag
     1021 actcgatctg gcgtccgtac gcgcccgctt ccttgccggc cttgagcact gcatcctgca
     1081 gctgcttgtc gagcttcttg aacgtcttgc ccgagaagca gagcggccgg atggtgatcg
     1141 cgtgctgcgt catggagagg tgcggcgcaa cctcgtagaa cttcatctgc tcgacgcccg
     1201 cggcctcgtt ctcgccggcg gcgatgacgc cgttctgaat cgcgttgtag acctcgttat
     1261 acgcaatcac ggtcggcgcc atgcccgcgg cctggaacgt gcggctccag atcggcgcgc
     1321 cctggacgcg gaccttgagg cccttcagct cctgcatgtt cttgaccggc ttgtttacga
     1381 agatgttgcg cgtgccgcca ccggcgtagc cgatcagcat gacgtcggcc ttctgcgcga
     1441 tctcgtcggc gactggcttg agcacgtcct ggtcgagcac cgcgttccag tgcttgaggt
     1501 cgcggaacag gaacggcgcg tcgatgaagg gcgcagcccg cgagaaggtc gacatgtgcg
     1561 ccggcgacac gatggcgtag tccacggcct tgccttgggc catgtactcg aaatactgct
     1621 tctcgaggcc gagttcgcta ttcttgtgaa gcacgaagtt gaccgccttg ccgccggagt
     1681 acttcttcac cagctcctcg aacttgacca ttgccttggt gaaggcatgg tcgtcgttga
     1741 actgcgatgc gccgtgcagc gtgagctgcg cctgcgccgg aactgcgaac gctgcggcga
     1801 tcgcagcgag aacaatgcgc ttcatgtcgg tttctcctcc ttgatggtga tacgccaaag
     1861 tgtacgcggg tccgaggggt cggtgagcgt cgcggagtga agcagggacg cgttgtccca
     1921 gatgacgacg tcgcccaccc cgtacttgag gcgcagctgg tatttctcct gcgtggcatg
     1981 gcccttgagc tcgtccaaga ggtcgagcgc ctcatcgtcc ggcattccct cgatgcggaa
     2041 agagctcccg gagacggcgt agagcgcggt gcggccggtc accggatgct tgcgcgccac
     2101 cttgtgccgc acccagttca tcttctgctc ctgctcttcg gtgagcaccg aagccacggt
     2161 gcgcgagcgc ttgtcctggt catcgcgatt gccgtagtga tgcagcgcaa caagcccttc
     2221 gatgcgcttc ttcatgccgg agggcaggcc atcgtaggcc gcgtactgat cggcgaatag
     2281 cgtatcgccg cccttcttcg gcacctggat cgagtacagc gtcgtcgcgc gcgccggcac
     2341 atcgaggtac gagtaatcgg tgtggaagta cgtgccggcg tcggcgagtc cgatcggctt
     2401 gccgtcttgc tgcacgttgg aaaggatgag gatgtcggcg tgctcgggat ggtggaactg
     2461 atcgataacg tgcggctccg gctggccgaa ctgcctggcg aacgcgagaa agcccgacgg
     2521 cgtgaacttc tgcccccgga ataccagcac ctgcgaagcg aagaacgcgt tccacacctt
     2581 gccgaagtcc ggatcgatcg tctcggcttc ggcgccgaac gaacccagct tcctcaaccg
     2641 catccgtgca ccgccagaag ttgcttcatg cgcgcgcagg cgagcggtgg gtccatgagg
     2701 aggcccgcag catccgccag gcggaagagt tccgaaagat gctggatgtt gcgcgccgac
     2761 tgctgcccgc caaccatgac gaagtggtcc tggtggccgt tcagccacgc cataaatacc
     2821 acgccggtct tatagaagcg cgtcggcgcg ccgaagatgt ggcgggagag cgggacggtg
     2881 ggcgccaata cttcgtggta ctcggccatt cgcatctggg cgaggaacgc caatgcctgc
     2941 gaagccgccg gcgcgatggc gtcgaagatg ccgagcagtg catcggaata gccgcgctcg
     3001 tcgccctcga tgagctcggc aaagttgaaa tcgtcgccgg tgtacatgcg cacgcccttg
     3061 ggcagcttgc ggcgcatggc gacttccttc cccttgtcga ggagcgagat cttgatgccg
     3121 tcgaccttgc gcgcatgctt ctcgaggatt gaaaggcata cctccatcgc ggcgtaatgg
     3181 tcgcgctggc cccagtagcc ttcgagcgcc gggtcgaaca tttcgcccag ccaatggatg
     3241 atcaccggct ccctgacctg ggacaggatg cgctcgtaca ccttgccgta gtcctccgga
     3301 gatttcgcag aggcggcgag cgcgcggctc gccatgagaa tgatgcggcc gcccagcttt
     3361 tcgatcgccg cgcactgctc ctcgtacgcg gcgatgatgt ccgccaagga atgcacgcct
     3421 gcgggcagat ggtcggtgcc tgcgccggag gcgacgatgc cgccgccttc ggccaccgtg
     3481 cgccggatga gctcgagcga attcttccaa tcgaggccca tgccgcgctg tgccgtatcc
     3541 atcgcttcgg cgacgccgaa gccccacgac cacaggtgac gccggtacgc gagcgtcgcc
     3601 tcccagtcga tcgccgcctc gagccacggc tctttcgtcg acaacggatc ggcgaccaca
     3661 tgggccgcgg cgagcgccct gcgggtgaac ggcgccttcg gcttctcgaa atgcccgggc
     3721 tcgcgcacgg catagtcgcc gcttggcagc ctgatcgtga gaacggtatc gcgcttcatg
     3781 gcttgagcct cggaacgtcc agccattttc gttgcttcca gctcttcagg ccaagctcgg
     3841 cgagctgcac gcccttggcg ccctcgagca ggttccagcg gaaaggccct tcgccggcga
     3901 cgtgcttaag gaagagctcc cattgcacct tgaacgcgtt atcgtagttc tcgtccggca
     3961 cttcctccca accctcgtag aagttgatgg gctgcgggat gtccgggttc cagaccggct
     4021 tgggcgtctt gtcgtaactt tgcaccaggc acttgcgcag tcccgcaacc gcgctcccct
     4081 gcgtgccgtc gacctgcagc gtgagcagat cgtcgcgccg cacgcgcacg tcccaggaac
     4141 tgttgaagtg cgcgacgacg ttccccttca ggaggaaagt cgcgtacgcc gcgtcgtccg
     4201 cggtggcctt gtatttcttc cctttctcgt cccagcggta cgggatatgc gtggcgccga
     4261 ggcagctcac cgcctgtact tcgccgaaga ggttgtccag cacgtagcgc cagtggcaaa
     4321 gcatgtcgag gatgatgccg ccgccgtcct ccttgcggta attccacgac ggccgttgtg
     4381 ccggcacacg gtcgccctcg aacacccaat agccgaactc gccgcgcacg gacaggatgc
     4441 ggccgaagaa gccttcgtcg cgcagcttgc gcagcttgag gagccccggg agccagagct
     4501 tgtcctgtac cacgccgtgc ttcaccctcg ccttcttcgc gaccgcgtac aagtccttcg
     4561 cctccgcgag cgtgtcggac acgggcttct cgcagtagat gtgcttgccg gccgcgatcg
     4621 cctttttcag gagcttggga cgaagctgcg tcgaggcggc gtcgaagaag atctcgtccg
     4681 ccgacgcgag cgccttgtcg agatcggtgc tccagcgctg cacgtggtgc ctgcgcgcga
     4741 gttcctcgac cttggccgcg ttgcggccca ccaggacggg atcgggcatg acgcggtcgc
     4801 cgttcttgag cgcgacgccg ccctgcttgc ggatctcgat gatggagcgg atgaggtgct
     4861 ggttcatccc catgcgcccg gtcacgccgt gcatgatgat cccgatgcgc ctttcgctca
     4921 ttttccgacc tcgatgaatt cgagggtcac accgtcggca tcgcgcgcgt acaccacttt
     4981 gcgtcccttg ttcgggccct tgtcgatcgc gacgggcggc gcgatcggct tcaccccgta
     5041 acgctgcact gcctcggccg cggccgcggc gtcatcgacg ttgaacgcca cgtgcgcaaa
     5101 accggtgtcg cacggcctgg cctttacgct tgccttcgtc gccggcgagc gatactcgat
     5161 gagctcgagc gtatgcccgg gcgccttgag gaacgcaatg accacatcgg cgttctcgac
     5221 gccggtgatg cgcgagacga gcgccggatc gcgcggcgcg cgggaaacca gctcgaaacc
     5281 gaggcagtcg cgaaagaagg cgacggtgcg gtcaagattc gagacggtga agctcgtgtg
     5341 attcgttccc agtacccgga agctcaagcc atcctcctca tcgattgcca atcgggcatg
     5401 gcgcggctcg cgtagcccgg acagtccagc gttttcatct gaatgattcc tttgtggatg
     5461 cgcagccgga ccgccccatg gcttcgctcg tagaggtccg ggtgcgcgct gccgaagtct
     5521 tgttgttcct gagcaggcag cccggccatg ccgttcacat agtggtggcc attgcgctcg
     5581 acgtgcgtga gcccgaggat cgaaaccagc gccaggtcct gctgcaacgc gagccccggc
     5641 tgaatcgtga ggtcctcgcc ggtcatgaag tagtccttgc cccacgcagc gcaacgcgcc
     5701 gcattgagga gcgacttgta gagacccttg cacgtcttgg acgagacgcc gcggtagcca
     5761 agcgccctag cgcgcggcaa cgcgtcgagc gaatcatcgg actcgtcgat gatgacgggc
     5821 ttcctcgcgc tcagcgccgc gatgttcgcg gcgagcgcct tctggcgctt gatcggctgc
     5881 tcgacgaaga cgatgctcga agcgagccgt ttgagcctgg gctcggtctc gatcgcgttc
     5941 cacagggcga tgactccctc cacatcctcg tattgctcgt tgccgtcgag cgatgcgtgg
     6001 tagggctcgg caatgcgatc gagcacagcg gcgatcgcgc gcaggcgctc gacatcggcc
     6061 ttcacatcgc cgccgacctt gagcttgaac cagcgatggc cgtagcgcgc gaccacttcc
     6121 tcgagcgtct cgggcaggcc gtcgttcacc ggtttcttgt tgtccgccga ggtgatcggg
     6181 tccaccagcc cgacggtgtg acgcgcggcg atttccgagc gcggctcgag cccggacagg
     6241 aagccttgca tgtcgagatc ccggaacgca atgcccgcaa cattcgtttt cacggcctga
     6301 tagaaggaga tgccgagtgc gcggcacagc gcgtcgagca gcgcgcggtc gagcagcgcc
     6361 gggccgtagt tctccaccag gccgagcgta ggccgggaat ggccgaacgc ggtgttcatt
     6421 cctgcatcca ggaccgcgtt ccgggccgat cgcatcgaag cacgcagctg ctcgaagtta
     6481 tcttcgttgc tcagcgccag attcttgtcg aaccatttcg gcgcgaggag ctctgcggcc
     6541 gctccctggg actgcttgcc attctccaat tcaatccttg caagcgcgaa ggcctggggc
     6601 gcctccgtaa gcgtcacgat gccgaagcga aacggcatgc gcagacgcac gtcgcgctcg
     6661 aagaacgaga tctccttcac ggagaacctc atgtttcgag cgcctccatg attcccttga
     6721 tgtacccgat ggcgcgcgcg gcggcgcccg gaccgtcggg cacgtaatcg aaaggctcga
     6781 ctgccacgtc gccgcgatag ccatgccttt tcagcgacgc aaagagcggc gcgaagcgct
     6841 gctcgccctg gccgggcccg cgccgattgc ggtcgttgac ctgcacgtgc gcaatcacgc
     6901 ccttcggcag ccagcgctcg acgagcgcgt tgagcggctc tttctccatc cgcccagccg
     6961 agctgcaatc gagcatgctc ctgacggcag gactgccgat ctcctccacc atgcgggaag
     7021 cttcatcgag cgtgttgata agcggcgtct gctccgaaga gagcggctcg atgcaataga
     7081 ccacccctgc tttctgcgcg cgctcggcga tcgaggcgaa gctttcttgc gcgcgcgcca
     7141 tggcggccgc gcgcgtctca ccggggtcga cgcgccgttg gtgcggcgag ccgtgcacca
     7201 ggtagcggcc gcccagctcg gcgcactggt cgatgaaggc gagcatcacg tcgatggtgc
     7261 gcttcctgac cttctcgtct ttgctactga tcgagagacc cgcgggcttg agcagcagcc
     7321 agtggagccc ggtgacggcg acgcccgcat cctcggcggc gacacgcgcc gccgcgatct
     7381 gcgcgcttcc caggcggtgc ggctcgtccg acaacgtgta cggtgcgatc tcgaggccgt
     7441 cgtagccgag cttggccgcg tactcgcact gccgcggaaa cggcatgggc gcgatcacct
     7501 cgttgcagag cgcgattctc acgtgcgcct ccaggctttt ctcatgacca tgaatatggt
     7561 gaagagcacg atcgcgaaga tcaccgcgct gatcggccgc gtgaagaacg gcgcgagcga
     7621 gccgtcggag aggacgagcc cgcgccgcag gctcttgtcg acgatgtcgc cgagcacgag
     7681 gccgagaacg aagggtgcca tcgggtagcc gagctttctc agcacgaatc ccgccacgcc
     7741 gatcgccagc atgatgtaca catcgaacag gcgcgaggcg atcgagaacg agcccacggt
     7801 gcacagcacg aagatgatcg gcatgatgat cgagcgcggt acctggatga tcttgaggag
     7861 cgggcgcacc atgaacaggc cgaagaacag gatcgccaac gtggcgaaga gcgtcatcgt
     7921 cactacgtcg tagacgaact gcggctggct caccatcagc atggggccgg gctgcacgcc
     7981 gtggatgatc atcgccgcca tcagcaccgc cgcaggcgcg gaccccggga tcgcgagcgc
     8041 gagcgccggg atgatgccgc ccgggatgga ggcgttgtcg ccggtctcgg cggcgatcag
     8101 accctcgacc gagcccttgc cgaattgctc gcgctccgcg ctggcgcgct tggctgctgc
     8161 gtaggacgac cacgcggcca tgtcctcacc cacgcctggc aggatgccga cgtagacgcc
     8221 gatcaccccg gagcgcagca cggtcttcca atagcgcagc acgtcgtgga tccgcggcag
     8281 caccgaatcg acgctgttca cgatgggctt cgcctgcggt gcggtcagaa ccgtcaggat
     8341 ttccgcgaag ccgaaggcgc cgatgagcgc cgggatgagc gagatgccac cggcgaggtc
     8401 gcccgagccg aacgtgaagc ggttaaaggc atggatgcct tcctggccga tctgcgccgc
     8461 aaagaggccg aggaagccca tgatccagcc cttgagcgcg tcgtctccgg tgaggcttcc
     8521 ggtcatcatc acaccgaaca cgccgagcca gaaaaactcg aacgcgccga aggagagcgc
     8581 aacctcgccc agagccggcg tgaagagcgc gaggcagatg acgccgaaga gcgagccgac
     8641 gaccgagccg gtggtcgcga tgcccatggc gcgcccggcc tggcccttct gcgcgagcgc
     8701 gaagccgtcc aggcacgcgg ccgcgttcgc cgcggtgccc gggatgttga gcaggatcgc
     8761 ggtacggctt ccgccgtaga tcgcgcccac gtacacgcag atgaggatga ggatggcgtc
     8821 gttcggcgcc atcttgatcg tgagcgtggt catgagcgcg atgccgagcg tcgccgagag
     8881 ccccggcatg caaccgatga gaatcccgac gagcgtcgcg ccgagcgtgt acacgatcgg
     8941 ccagaacgcg gcgaaattgc cgatcgacct gccgagcgcg ctcaagcctt ccattcacgg
     9001 cagcctgatc agaaacagtt cctggaatat gagcgaaatg gcgacgctcg ccgcgagccc
     9061 gatcgagaca gaaatggcag cgagccgagg cagttcacct tttgccctcc gctcccggta
     9121 ctgcagagtc cagatcatca ccgcgatgaa gatccacgcc gcgagccaga acggaatgcc
     9181 gcgcccgacc attcccgcgg cgaatccgag gcagagcgcg agcggcagga ggatgcgccg
     9241 ccattcgatc gcttctgctt cttcttgtct tgctcgcagc agcattgcga tgccgagcag
     9301 aacaatgaaa gcgcccagga tcccgggcag caatcccggc gccgagaaag gcttgacgcc
     9361 gaggttggcg aggcgatcca tcgtccacga ggcatagaag atcgccaagc cgagcgcgat
     9421 ccagaacgcc ccctggagac gatcgctctt cattcaggct ttgggatacc taccgtatcc
     9481 ggcgagacct tcgtcttgcc gcccgcatgc agcagccatg cgttcgcctg caccgccggc
     9541 agcgccgcct tctgcgcgtc ctgtcccgac atcggcgcga acagcgctcc gcggctcgtc
     9601 gcgtacttct tcagcgcctc cgagcgcgtg atgtggtcgc cccagacgcg gtcgagcgtc
     9661 gccacgacct gcggcggcac gcccttgggg acgaagacgc caaagtagtt ggtcgggatc
     9721 ttcatcccgg gcagggtctg cgtaatcggg tcgatcctgc catggccctc gatctcgagc
     9781 ggcttatccg cgaccgcggc gagcgggcgc aggcgcttgc cgcgaatcat ctcggcctgc
     9841 tccaccgcga gctgcgtggt gacttgcgtt tcgccggcga cggtcgcgac caccgccggg
     9901 ttgccgccgt cgtacgtgac atggcgatac ttcacgcccg tagcgcgcgc gatcgcctcc
     9961 atcgcgttgt gcccgcccga agtcacgcca gccgtcgcca cggtgacttc gcccggcttg
    10021 gccttcatcg catcgagcag gtcctgcatc gtcttgtacg gcgtgttggg attcacgccg
    10081 acgagcggca cgttggccac gctcaggaaa aggtgccagt cggtgaggtt cgtctcgagc
    10141 attccgagcg cgcggtagac cccgaggtcc tgggccgcgc ctgccgtcca cgtatggccg
    10201 tccttcggcg cgtcgagcgc gctcttggtg ccgatcgaac cggatgcgcc gggctggttg
    10261 acgatcacca ccttctgccc gagatgcttc tcgagctcag cggcggtaat gcgcgtcact
    10321 tggtcggtcg atccgcccgc cgcccacggc acgatgatcg tgaccggctt cgccggcttc
    10381 cagttctgcg cctgggccga cgctgcgagc agcgcggcgg cgcatacgac aacggttctg
    10441 ttcatgttca tctccttatg ccaatcgatt cgggggagac cttcaccatg ccggcctcct
    10501 gaagcatgta ggcgtcttcc accaccgtgg gccacaccag gtcatgtgcc gccttgccga
    10561 ccgcgggcgt gaagagcgcg ccgcggcttt gcgcgtactg gcggatcttc tccgatttcg
    10621 ccacgcgctc cgtccaaatt ctgtccatcg tcgccgcaac ctcggcggga acgcctttcg
    10681 gaatgaagat gccgaaagcc gtcgtgacct tcgggaagcc aggcagtgtc tgcgtgatcg
    10741 gcgcgatggt gccgacgcct tcgatcgtga gcggctgatc ggcgaccacg gcgagcggcc
    10801 gcaggcgctt gcccttgatc atctcggcct gctcgaccgc aagctgcgta gtgagctcgg
    10861 tctcgccagc gaccgtcgca acgactgccg ggttgccacc gtcgtacgtg acgtggcggt
    10921 acttcacgcc ggccgccttg gcgatcgcct ccatggcgaa atgccccgag gacgtgttgc
    10981 cagcggtcgc gactttcacc tgccccggcc gcgccttcat gtcatcgaga agctgctgca
    11041 ggctctggta gggcgtgccg ggattgacgc tgacgatcga gacgttggtg accgagagat
    11101 acagatggaa gtcgtcgacc ttgctgttga gcatgccgag gagcgggtag gtgccaagct
    11161 gctttgcggc gcccgcagtc cacgtatggc cgtcctttgg cgcttccatc gcgttgcgcg
    11221 tgccgatgga gcccgagccg cccggctggt tgaccaccac gatcttctgg ccgagcgcgg
    11281 gctcgagctc ggcagccgcg agccgcgtca cctggtcggt ggcgccgccc gccgcccacg
    11341 gcacgatgat cgtgatgggc ttggttggct tccagttttg agcctgcagc ggcaacgcca
    11401 gcagcgcccc ggcgaataac gtcaagaacc tgctcatgtc acctcctcaa tgcgttgaga
    11461 atcatgtccg agatatgccg cgcgcggccg gcgagagcgc gcggcgccat cagatcgcgc
    11521 ccgaagatgc gcgagagcgt gtagcggttg ctgagataga agtagccttc gcccgcgatc
    11581 gagatataga gctgcaccgg atcgacgccc ttgcggaaca ggcgctgcgc ttcgccccgg
    11641 cgcaacaccc gacgcaagag ccccaccagg tgcgaatgca tctcgggaac gcgccgcgag
    11701 cggcgcacgt ggcgcccctt gtgcaggttc tcgctgttga gcagcgtcat gaactccgga
    11761 tgttcgagaa agtagttcca ggtgaattcc accaggcgcc gcaccgcgat gcgcggatcg
    11821 agatgctcga gctcgagcga gcgctcggcg ttgcgaatgt gtgcgtaacg gtcttccagc
    11881 gcggcgagga acaaaccttc cttgtcgccg aagtagtagt aaagcatgcg cttgttcgcg
    11941 cccgcgcgtg ccgcgatccg atccacgcgc gccccgccga ggccgtggcg cgcgaactcc
    12001 gcggtggcgg cttcgagaat cttctgctgg gtatgattca ctccatcccc agcagacggt
    12061 aaacgcggcg tgtgtactcg ccgaagcgct cgttcgtctt catgtcgagc gtgcgcgggt
    12121 acggcagctc gacctcgaag ttgtcggcca tgcgcgcagg ccccgcggtg agcacgacca
    12181 cccgggtgcc gagaaacacc gcctccgaga tgccgtgcgt cacgaacacg atggtcttgc
    12241 ccgcctcttt ccagatgcgc agcagctcga gattcatctt ctctcgcgtc agcgcatcga
    12301 gcgcgccgaa gggctcgtcc atcagcacga gcttcgggtc gtggaccagc gctcgcgcga
    12361 tcgccgcgcg ctgctgcatg ccgccggaga gctcgtaggg atacttgtgc tcggcgccgg
    12421 cgagcccgac cagcttgaga agatgccccg cgcgctcgcg cgcttccttc ccgggcaagc
    12481 ccaggatctc ggccggcagc agcacgttct cgaggacgcg gcgccacttt agaagcagcg
    12541 gcgcctgaaa caccatcccc acgtccttcg ccgggtcgaa cggatgggtg ggtgagccga
    12601 tacgcacctc gccgccgtcg tgcggatgca ggccggccag gatcttgagc agcgtcgact
    12661 tgccgcagcc cgagggcccg acgagcgaga ccagctcgcc cggcagcacg tcgaaggtcg
    12721 catcggaaat cgccaggtgc tcgccgtaat tcttccttac ccccgaaaga tgaataaacg
    12781 gctcacgcaa cgcgggcgtc ctttacaacg aggatgcgct cgaggatcaa cacgaggccg
    12841 taaagcagca tgccgagcag cgtgatgagc agcaccgcca tgaacatggc gggcgtatcg
    12901 agcgtcacct ggacctgcag catgaggtac cccaggcctt tgtccgagcc gaggaactcg
    12961 ccgacgatcg cgcccgccac cgccaggatc gcgcccacct tcatgccgga gaagatgtag
    13021 ggcaaggcgc ctggcagttg gatcttgacg aagagctgcc agcgcgagcc cttcagcgag
    13081 cgcacgaggt ccaggagctc gggctcgatc tcgcgcaagc cgcgcgcggt cgtgaggagg
    13141 atcgggaata cggcgatcga gaacgccatg atcgagttcg ggaacacccc gtacttgaac
    13201 cagacgatga tcagcggccc gagcgccacc ttcgggatca tgttgagcga gacgagcagc
    13261 ggcatgaaga acgcttccag cgtcttcgac caggtgaaca ccagcgcgag cgcgacgccg
    13321 accaccagcg cgatcagata gccgacgaag atctcggtgc cggttaccgc gatgttggtc
    13381 caccagtcgt agttgggctg gagcagcgcg tcgagcgtgg cgcccggcga gggcatcacg
    13441 aatttcggca cctcgccgaa gcgcacgaag agataccagg cggccagcag tacgagatgc
    13501 gccagcactg cgagcgtcca gcgtccgact ttggaattca ttcgagccac ctttcgagat
    13561 tcgcgcccac gaaagcatcg tcggaaagat cggcgtgctc ccgatagacg cgatcgatct
    13621 ccgcagcctg ccccggcgat agcttttctt ttggatcgag gcaccaggtg ccggcgagca
    13681 gcccttggcg gcgcaggact tcgtggcagc ccgggatgca gcccgcgaaa ttgtgcgcca
    13741 cgtcgaagaa cgcactgttg cagtcggtga cgcgcgcgtc gagagccaac aggtcttcgg
    13801 acagccgttc gctctttgtc cgatcaaaca gttgaaccgc tgtcttggtc cacacgctcc
    13861 agtgcccgag cagtccgcca gcaatacgca cccgaacgcg ctccccgtcc ctcgggacat
    13921 cgaaaggcac ggcgaggtcc agaacgatgt ggtcgtcgtt gccggtatag agcgcgatgc
    13981 gttcctcggc gcgtgcggcc accacgcccc tgatcacgtc cagcgtcgcg tagcggttga
    14041 acggcgcgat cttgatcgcc gccacgttct cgatagtggc gaagcgccgc cagaattcca
    14101 tcggcagcgc gatgccgccg accgccggct gcaggtagaa gccgaccagc gggatctcct
    14161 ctgcaaccgc actgcagtgg cggatgagcg cgtcgatcga tgcccccttc atcgccccga
    14221 gcgaaagcat gcccgcgtga tagccgagcg agcgcgcgag ctgcgcctcg cgcttcgccg
    14281 cgctggtctt tccggccagg ccggcgatca gcacgactgg gcgcttagcc caagtcttcg
    14341 tttcttccaa tgcaattcgc aagacaggct cgtagagccc ggc
//
LOCUS       DASTPD010000327         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_587494, whole genome shotgun sequence.
ACCESSION   DASTPD010000327 DASTPD010000000
VERSION     DASTPD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587494"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /gene="carB"
                     /locus_tag="VFR57_10900"
     CDS             <1..1812
                     /gene="carB"
                     /locus_tag="VFR57_10900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0259911.1"
                     /translation="DQNLSQIDKDQLLELKRKGFSDRRLAYLFNTTEQEVRAKRHKLE
                     IRPVYKRVDTCAAEFASKTAYMYSTYEEECEAQPTSRRKIMVLGGGPNRIGQGIEFDY
                     CCVHAAFALRDDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVEKEKPWGV
                     IVQYGGQTPLKLARDLAAAGVPIIGTSAESIDIAEDRERFQQLLEKLKLRQPPNRTAR
                     STEEAVKHAAAIGYPVVVRPSYVLGGRAMEIVHQQSDLERYMKEAVKVSNDSPVLLDR
                     YLSDAIEVDVDCVSDGKDVIIGGIMEHVEEAGVHSGDSACCLPPHSLASHLERELRRQ
                     TAQMARALKVKGLMNVQYAIQGGTVYVLEVNPRASRTVPFVSKATGQPLAMIGARCMA
                     GKTLAEQGAVEVTPQYFSVKEAVFPFARFPGVDTMLGPEMKSTGEVMGVGETFGEAFV
                     KSQLAAGVKLPEGGRAFVSVRDADKLAAVQVARDLVALGFALVATRGTAAVFRSHGIP
                     LTEVNRVSEGRPHIVDMIKNGDISFVVNTEEWTRTAQSDSRSIRTTALARRVTYFTTV
                     AGAKAACAGMKYLANLEPYRLQDLHQRLRQATPETVA"
     gene            1809..2282
                     /gene="greA"
                     /locus_tag="VFR57_10905"
     CDS             1809..2282
                     /gene="greA"
                     /locus_tag="VFR57_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0259912.1"
                     /translation="MMKHPMTKRGAELLRTELHRLKTVERPATTQAIAEARGHGDLSE
                     NAEYEAARDRQGLIEARIRDIETKLANAQIIDPKLLQTEGRCVFGATVEVAEASGETA
                     TWQIVGEDEADIKEGRISVSSPIARALIGKEAGESVEVQTPGGLKRYEIIDVRYE"
     gene            complement(2654..>3129)
                     /locus_tag="VFR57_10910"
     CDS             complement(2654..>3129)
                     /locus_tag="VFR57_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011168770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0259913.1"
                     /translation="EDLRAHLTLLDARIEGYDRELEAQAKLSERARRLMQIRGIGPLT
                     ALAIVATVGDARDFRSGRQFAAWIGLTPKQFSSGGKNRLGPITKRGDAYLRGLLVQGA
                     RAAMHTALRHKDRLSRWACALRERRGYRRTVVAMAAKNARIAWALLSRDQEFRMA"
BASE COUNT          616 a         1025 c          973 g          515 t
ORIGIN      
        1 gaccagaacc tttcccagat cgacaaagac cagcttctgg agctcaagcg caagggattc
       61 tcggaccggc gccttgccta cctattcaac acgaccgagc aggaagtccg cgcgaaacgc
      121 cacaaactgg aaattcggcc cgtttacaag cgggtcgaca cctgcgccgc cgagttcgcg
      181 tcgaagacgg cttacatgta ttcgacgtac gaagaggagt gcgaggcgca gccgacgtcc
      241 agaaggaaaa tcatggtgct cggcggcggg cccaatcgca tcgggcaagg catcgaattc
      301 gactattgct gcgtgcacgc cgctttcgcg ctgcgcgacg acggcttcga gaccatcatg
      361 gtcaactgca acccggagac cgtctcgacc gactacgaca cttccgaccg gctgtacttc
      421 gagccgctca cgctggaaga cgtgctcgag atcgtcgaga aggaaaaacc ctggggtgtg
      481 atcgtgcaat acgggggcca gacaccgctt aagctggcac gcgacctcgc cgccgccggc
      541 gtaccgatta tcggcacgag cgcggagtcg atcgacatcg ccgaggaccg cgagcgcttc
      601 cagcagctcc tcgagaagct aaagctgcgc cagccgccca accgtacggc gcgttccacg
      661 gaggaagcgg tgaaacacgc cgcagcgata ggctacccgg tggtcgtgcg cccgagctac
      721 gtgctcgggg gccgcgcgat ggagatcgtg caccagcaat ccgacctcga gcgttacatg
      781 aaggaggcgg tcaaggtctc gaacgactcg cccgtgctcc tcgaccgata cctgtccgat
      841 gcgatcgaag tcgacgtcga ctgcgtatcg gacggcaagg acgtcatcat cggcggcatc
      901 atggagcacg tggaggaggc cggcgtgcac tccggcgatt cggcgtgctg cctgccgccg
      961 cattcgctcg cgagccacct ggagcgcgag ctgcggcgcc agaccgcgca gatggcgcgc
     1021 gcgctgaagg tgaagggcct gatgaacgtg caatacgcga tccagggcgg cacggtctac
     1081 gtgctcgagg tcaacccgag ggcttcgcgc accgtgccct tcgtgtccaa ggccaccggc
     1141 cagccgctcg ccatgatcgg cgcgcgctgc atggccggca agacgctggc ggagcagggc
     1201 gccgtcgaag tgacgccgca gtacttttcg gtgaaggaag cggtgtttcc gtttgcgcgc
     1261 ttcccagggg tggacaccat gctcgggccg gagatgaaat cgaccggcga ggtgatgggc
     1321 gtgggcgaga ccttcggcga ggccttcgtc aagtcgcagc tcgccgcggg cgtcaagctg
     1381 cccgagggcg ggcgcgcctt cgtcagcgtg cgcgatgccg acaagctcgc cgccgtgcag
     1441 gtggcgcgcg acctcgtcgc gcttggcttc gcgctggtgg cgacgcgcgg cacagcggcg
     1501 gtattcaggt cgcatggcat tccgctcacg gaagtgaatc gcgtctcgga aggccgcccg
     1561 cacatcgtcg atatgatcaa aaacggcgac atcagttttg tggtgaacac agaggagtgg
     1621 acgcgcaccg cccaatcgga ctcgcgttcg atccgcacca ccgcgcttgc gcgccgcgtc
     1681 acctacttca ccaccgtggc gggcgcgaaa gccgcgtgcg ccggcatgaa atacctcgcg
     1741 aacctcgagc cctaccgcct gcaggacctc caccagcgcc tgcgccaggc gactcccgaa
     1801 acggtagcct gatgaaacat ccgatgacca agcgcggcgc ggagctgctg cgcactgagc
     1861 tccaccgcct gaagaccgtg gagcgccccg cgaccacgca agcgattgcc gaagcgcgcg
     1921 gccacggcga cctctccgaa aacgccgagt acgaagccgc gcgcgaccgc cagggcctga
     1981 tcgaagcgcg tatccgcgac atcgaaacta agctcgccaa cgcgcaaatc atcgacccga
     2041 aacttctgca gaccgaaggg cgctgcgtct tcggcgccac ggtcgaagtc gccgaagcct
     2101 ccggcgaaac cgcgacctgg cagatcgtcg gcgaggacga agccgacatc aaagaaggcc
     2161 gcatctcggt gagctccccc atcgcccgcg ccctgatcgg caaagaggcg ggcgaatccg
     2221 tcgaggtcca aacccccggc ggcctcaagc gctacgaaat catcgacgtc cgctacgagt
     2281 aggcagtaaa gggcgcgccc cgcgccttat cgctttgtcc cgcgccgccg cgtactccgt
     2341 ccttgggcgt cacgacgttc gccggcacac cgtcaactaa atcttttcgg atgccagtca
     2401 actaatttgt caaccaatac actataagga ctcccctgcg gagcaaagct tgttgaagtg
     2461 aagaggtcgg actgcgtcac tataaacggc ctgttcgcga gcaaagttgc tctggccctg
     2521 atgaaagccg cgcgctcggt cctcgcacgt tagtcggcat cgaatgccgc caagaattgg
     2581 ttcagggtgt ccgagcgtgg gttcgaccaa ttcgatcatc aacgtttgct gtctcgttta
     2641 gggtgtggtg cacctatgcc atacgaaatt cctggtcgcg tgatagcagc gcccatgcga
     2701 tgcgcgcgtt cttagccgcc attgccacga cggttcttct gtagcccctg cgctcgcgca
     2761 gcgcgcaagc ccaacgcgat agccgatcct tgtgcctgag cgcggtgtgc atcgcagctc
     2821 gagccccttg cactagtagg ccgcgcaaat aagcatcgcc gcgcttagtg atgggcccga
     2881 gacggttctt gccaccggag gagaactgtt tcggtgtcag ccctatccaa gcggcgaact
     2941 gccgcccact cctgaaatcg cgcgcgtctc ctacggtcgc gacgatcgcg agcgccgtca
     3001 aaggaccaat gccgcggatt tgcataaggc gtcgcgcccg ttcgctcagt ttcgcttgcg
     3061 cctcgagctc gcggtcgtag ccctcgatac gcgcatccag caatgtgaga tgagcgcgca
     3121 gatcttcga
//
LOCUS       DASTPD010000328         8897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1406853, whole genome shotgun sequence.
ACCESSION   DASTPD010000328 DASTPD010000000
VERSION     DASTPD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406853"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFR57_10915"
     CDS             <1..237
                     /locus_tag="VFR57_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU0259914.1"
                     /translation="FGRLMAFEAARARAHYDKALQVLPGADRRQQRPGLIMAAIYRTL
                     LDEIERDGFRVLTRRTSLTPLRKFWIAWRTWISA"
     gene            213..1466
                     /gene="hpnE"
                     /locus_tag="VFR57_10920"
     CDS             213..1466
                     /gene="hpnE"
                     /locus_tag="VFR57_10920"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEU0259915.1"
                     /translation="MAHLDKRVAIVGAGYAGMAAAVALAERGVRVCVFESGAVPGGRA
                     RRIRIPSDGQDPRLDNGQHILVGAYTELFRLMRRVGVPADALLRIPHEIRYADGFAFR
                     ALWLPAPLGLLAGLLALNRVPFAERIGALRFIAALRKRDWRMQPDVTVKELLDTHGEH
                     GRLRHYLWEPLCVSALNTPLDQASANVFLAALRDTLASERDASDLLLPRVDLSRLFPE
                     PAAEYVRARGGEVRLGEAVQDLALLRKDFPRIIVAVGPHQLKNVMPEADLGFTYQPIY
                     TCYLQYPESTRLEFPMLGIGQGLVQWVFDRGALLGEKGRLACVISAQGDHQQMTQEKL
                     AVACDIELRKALPNLPAALWTRVIAEKRATITCTPGLKRPPARTTTTGVFLAGDYTDA
                     EYPPTLEAAVRSGIRAADLVASSAK"
     gene            complement(1497..2840)
                     /gene="radA"
                     /locus_tag="VFR57_10925"
     CDS             complement(1497..2840)
                     /gene="radA"
                     /locus_tag="VFR57_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU0259916.1"
                     /translation="MKRPKTAYVCSECGASALQWFGACPSCGAAGTLSETVSERGSAR
                     VGRMSASTVALAEVQPKDLERIPTGIGELDRALGGGLVPGQVVLLGGDPGIGKSTLLL
                     QALCTARDGVLYLTGEESVEQVALRARRLGVETGGVRIAAETHLERALGALEASRPRI
                     AVIDSIQTLWSEILQSAPGSVAQVRECAAQLTRHAKRAGAALFIVGHVTKEGAIAGPR
                     VLEHIVDTVLYFEGDAHSAFRLVRAVKNRFGAVNELGVFAMTEKGLKGVANPSRLFLA
                     DHDKPVAGSCVLATLEGTRPLLVEIQALVDPAHTPTARRLSVGLEANRLAMLLAVLHR
                     HAGIATWEHDVFVNAVGGVRIGEPAADLAVCLAVVSSFTDKPIPAKVLAFGEVGLAGE
                     VRPAARGQERLREAAKLGFERAIVPKSNLPKANIPGLETLAVERIDQAVRLLRDL"
     gene            complement(2837..3910)
                     /gene="alr"
                     /locus_tag="VFR57_10930"
     CDS             complement(2837..3910)
                     /gene="alr"
                     /locus_tag="VFR57_10930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0259917.1"
                     /translation="MPRPIRATISSAALAQNLTVAKRYAGAAKLWAVIKANAYGHGVE
                     RAVRALSSADGFAVLDLREAERLRSAGVAKPILLLEGFFQAVDLELVQRLALTPVIHN
                     PEQVEIVKRTTLAAQTDVYLKVNTGMNRLGFGAESLRPAYEALRMHRQVRSIILMTHF
                     ADADGAGGISAQLQRFDELAQPLRAQGSSLANSAALIRFPEARGDWVRPGIMLYGCSP
                     FADRTAESLGLKPAMTLTSEIIATQRMQRGERIGYGLSYEADGAMSIGVVACGYADGY
                     PRHAFTGTPVLVNGKRSRIVGRVSMDMITVDISGIPEARVGTPVTLWGEGLSADEVAA
                     AAGTLSYELLCKLTARVPISEAP"
     gene            3977..5269
                     /gene="lplT"
                     /locus_tag="VFR57_10935"
     CDS             3977..5269
                     /gene="lplT"
                     /locus_tag="VFR57_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEU0259918.1"
                     /translation="MNYGFYIIMAAQFFSSLADNALLVAAIALLIQAEAPSWLTPYLK
                     FFFVVSYVVLAPYVGAFADRLPKGQVMLIANTVKIIGCLTMLWGSHPLIAYGIVGLGA
                     AAYSPAKYGILTEYLPHRKLVVANGWIEGLTVASIILGTMLGGLLIHVRVSTELLSWN
                     LPLIDTSIDTPAEAAIAIIIVVYAVAALFNLEVPQTGVALKKLPSHPIDTLKDFARCV
                     NHLWRDRLGQISLAVTTLFWGAGATLQFIVIEWARAALGFDLSKAAILQGVTAIGIAV
                     GAVLASILVTLDRSVRVIPLGIAMGLIVLAMNLVSQVWLAISLMVLIGGLAGFFVVPM
                     NALLQHRGHHLVGAGRSIAVQNFNENASILVMIALYSLLLGVGYSIYSVIVVFGLFVA
                     GTMTLVQLWFWRNRRVRHEEVDRLLAFARAEDPHKPRE"
     gene            complement(5278..5913)
                     /locus_tag="VFR57_10940"
                     /pseudo
     CDS             complement(5278..5913)
                     /locus_tag="VFR57_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(5901..6365)
                     /gene="rimI"
                     /locus_tag="VFR57_10945"
     CDS             complement(5901..6365)
                     /gene="rimI"
                     /locus_tag="VFR57_10945"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU0259919.1"
                     /translation="MSAVLKDIPRLNPMAERDLDEVMAVESAIYTHPWTRGNFADSLR
                     AGYECRVLRLGPELLGYFVLMVAAAEGHLLNLSIAAAHQRAGHGAWLLAQAAGLARKI
                     GARSLFLEVRPSNQGAQALYTRFGFRKVAVRRGYYPAHTGREDALVLALPLA"
     gene            complement(6349..7005)
                     /gene="tsaB"
                     /locus_tag="VFR57_10950"
     CDS             complement(6349..7005)
                     /gene="tsaB"
                     /locus_tag="VFR57_10950"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0259920.1"
                     /translation="MRFAAIETSTEWCSVAVWNDGEIAALERRAGQRHSEFALPMLQE
                     LIKGSGIDAVAFGAGPGAFTGLRIACALAQGLAFARSLPVIGISTLEALAQESGAARV
                     VACIDARMREVYYAALEKRDGRWHEIIPADCVAPRSAPLPPGKGWTGCGSGFEVYREE
                     MKAKVEVARPEIHPTAMAVAQLAAPRLAAGEGVDAAQAAPFYLRDKVAFTKEELERRP
                     "
     gene            7031..8110
                     /locus_tag="VFR57_10955"
     CDS             7031..8110
                     /locus_tag="VFR57_10955"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0259921.1"
                     /translation="MKEHSIAVIPGDGIGREVMPEGVRVLEAVGAKHGLRFKWQEFPW
                     SCDWYKAHGKMCPDDAPDILRRFDAIYFGAAGNPAIVPDHVSAWGLLINFRRWFDLYV
                     NLRPVRLFEGLPCPLAGRKPGDIDYVVIRENTEGEYGNVGGRVFENTENEIVMQQAVF
                     SRKGIDRIMKYSFELARSRKKHLTSCTKSNGITITMPYWDERFAEMKNNYPDVKADQY
                     HVDGLTIQMVLNPDRFDVIVASNLFGDILSDLGPATAGTIGIAASGNINPERTAPSIF
                     EPVHGSAPDIAGRRIANPIGAIWSGALMLEHLGHPRAAADVLRAIERALIEGPRTPDM
                     KGKASTSEVGTAIAEEIRTRSTLAA"
     gene            8107..>8897
                     /locus_tag="VFR57_10960"
     CDS             8107..>8897
                     /locus_tag="VFR57_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU0259922.1"
                     /translation="MKTYRIASIPGDGIGNEVIPAGMEVLNRVADSFALEFRHFDWSS
                     KRYVETGRYIPEGGLEELRKFDAIFFGAVGAPDVPDHVSLWGLRLPICQGFDQYANVR
                     PARVLPGVKSVVPDIDWVIVRENTEGEYSGSGGRVHQNLPEEIATETSVFTRKGVERV
                     HRFAFELARQRPRKHLTLVTKSNAQRHGMVLWDEIFFEVQKHYPEVQTDRILVDAMTT
                     RMVLKPGSLDTVVASNLHADILSDLAAALSGSLGIAPTANLNPER"
BASE COUNT         1466 a         3010 c         2903 g         1518 t
ORIGIN      
        1 ttcggccggc tgatggcgtt cgaagccgcg cgcgcccgag cccactacga caaggcgctg
       61 caggtcttgc cgggtgcgga tcgcaggcag cagcgcccgg gactcatcat ggcggcgatc
      121 taccgcacgc tgctcgacga gatcgagcgc gacggctttc gcgtcctcac ccggcgcacg
      181 tcgctcacgc cactcaggaa gttctggatc gcgtggcgca cctggataag cgcgtagcca
      241 tcgtcggcgc gggatatgca ggcatggcag ccgcggtcgc gctcgcggag cggggcgtgc
      301 gggtatgcgt attcgaatcc ggcgcggtgc cgggcgggcg ggcgcgccgc atccgtatcc
      361 cttcggacgg acaggacccc cgcctcgata acgggcagca cattctggtg ggcgcctaca
      421 cggagctttt tcgcctgatg cgcagggtcg gcgtgccggc ggatgcgctg ctgcgcatcc
      481 cgcacgagat caggtacgcc gacgggttcg cgttccgcgc gctttggctg ccggcgccgc
      541 tcggactgct cgccggactg ctggccctga atcgcgtccc cttcgctgag cggatcggcg
      601 cgctgaggtt catcgctgcg ctgcgcaagc gcgactggcg catgcagccc gatgtcacgg
      661 tgaaggagct gctcgacacg cacggcgagc acggccggct gcggcattac ctatgggagc
      721 cgctatgcgt ctcggcgctc aacacgccgc tcgaccaggc gtccgccaac gtctttctcg
      781 ctgccttgcg cgacacgctg gcgagcgaac gagacgcctc ggacctgctg ctgccgcgcg
      841 tcgatctttc gcggcttttc cccgagccag ccgcggaata cgtgcgcgcc cgcggcggcg
      901 aagtgcggct gggggaagct gtccaggacc tcgccttgtt gagaaaagat ttcccgcgga
      961 tcatcgtcgc cgtcgggccg caccagctga agaacgtgat gccggaggcg gatctcgggt
     1021 tcacctacca gccgatctat acgtgctacc tgcaatatcc ggagagcacg cggctcgaat
     1081 tcccgatgct cggcatcggc caaggcctgg tgcagtgggt tttcgatcgc ggcgcgctgc
     1141 tcggggaaaa aggtcggctc gcttgcgtga tcagcgcgca gggcgatcac cagcaaatga
     1201 cgcaggagaa gctcgctgtc gcatgtgaca tcgagctgcg caaggcgctc cccaacctgc
     1261 ccgcggcgct atggacgcgg gtgatagccg agaagcgcgc cacgatcacc tgtactccgg
     1321 gactaaagcg cccgcccgca cgcaccacga ctaccggagt attcctggca ggtgactaca
     1381 cggacgctga gtatccgcca acgctggagg ccgccgtacg cagcggaatc cgcgccgcgg
     1441 atctggttgc gtcatccgca aagtaagtag cgcgttgtgg gtatacctct tcggcgctag
     1501 agatcgcgca acagccgcac cgcctggtcg atacgctcta cggcaagcgt ttccaggccc
     1561 gggatgttcg ccttcgggag attcgacttc ggcacgatcg cccgctcgaa gccgagcttc
     1621 gccgcctccc tgagccgctc ctgaccgcgc gctgcgggac gcacttcgcc ggcgaggccc
     1681 acctcgccga aggcgagcac tttggcggga atcggcttgt cggtgaagct ggacacaacg
     1741 gccaggcaga ccgccaggtc tgctgcaggc tccccgatgc gcaccccgcc aaccgcgttc
     1801 acgaacacat cgtgctccca ggtcgcgatg cccgcatgcc ggtgcagcac cgcgagcagc
     1861 atcgcaagcc ggtttgcttc cagcccgacc gatagccggc gcgcggtggg tgtatgcgcc
     1921 gggtcgacca gcgcctggat ctcgaccagc agcgggcgcg tgccttccag cgtggcgagg
     1981 acgcaggaac cggcgacggg cttgtcgtgg tcggcgagaa agagccgaga agggttcgct
     2041 acgcccttga ggcccttctc cgtcatggcg aacacgccca gctcgttgac cgcgccgaag
     2101 cggttcttca ccgcgcgcac aaggcgaaaa gcggagtggg catcgccctc gaagtaaagc
     2161 accgtgtcga caatgtgctc gagcacgcgc ggtccggcga tcgcgccctc cttggtcacg
     2221 tggccgacga tgaagagcgc cgcgccggcg cgcttggcgt ggcgcgtaag ctgcgcggcg
     2281 cattcgcgga cctgcgcgac cgagccgggc gccgactgca ggatctcgga ccatagggtc
     2341 tggatcgagt cgatcaccgc gatcctcggc ctcgaagctt cgagcgcgcc aagcgcgcgc
     2401 tcgagatgag tctccgccgc gatgcgaacc ccgccggtct ctaccccgag tcgccgcgcc
     2461 cgcagcgcga cctgctctac cgattcttcc cccgtgaggt acagaacacc gtcccgagcg
     2521 gtgcagagcg cctgcagcag cagcgtcgac ttgccgattc ccggatcgcc gccgagaagc
     2581 acgacctgcc ccggcaccag cccgccgccg agcgcacggt cgagctcgcc gatgccggtc
     2641 gggatgcgct cgagatcctt cggctgcact tctgccaggg ccaccgtgga ggcggacatc
     2701 ctcccgactc ttgcgcttcc acgttccgat acggtttcgc tcagcgtgcc ggcggcgccg
     2761 cacgaagggc acgctccgaa ccactgcagc gcgctcgcgc cgcactccga gcaaacgtag
     2821 gcggtcttcg gccttttcac ggcgcctccg agatcgggac tcgcgcggtg agcttgcaca
     2881 ggagctcgta gctcagcgtc cctgcggcgg cggcgacctc atcggccgaa agcccctcgc
     2941 cccagagcgt caccggcgtg ccgacgcgcg cctccgggat gccggagatg tccaccgtga
     3001 tcatgtccat cgatacgcgt ccaacgatgc gcgagcgctt tccgttgacc agcaccggcg
     3061 tgccggtgaa tgcgtggcgc ggatagccgt cggcgtagcc gcaggcgacg acgccgatgc
     3121 tcatcgcgcc gtcggcttcg taggacaagc cgtagccgat gcgctcgccc cgctgcatgc
     3181 gctgcgtggc aatgatctcg gaggtgagcg tcatggcggg cttgaggccg aggctctccg
     3241 cggtgcgatc cgcgaatggc gaacagccat agagcatgat gccgggccgc acccagtcgc
     3301 cgcgcgcttc cgggaaccga atcagggcag ccgagttcgc cagagagctg ccctgcgcgc
     3361 gcaacggctg cgccagctca tcgaaccgtt gcagctgcgc acttatcccg cccgcgccgt
     3421 cggcgtcggc gaagtgggtc atcaatatga tgctccttac ctgccggtgc atgcgcagcg
     3481 cttcatatgc cgggcgcagg ctctccgcgc cgaagcccag gcggttcatc ccggtgttga
     3541 ccttaaggta gacatcggtc tgcgccgcca gcgtggtgcg cttgacgatc tcgacctgct
     3601 cagggttgtg gatcacgggg gtcagcgcga gcctctgcac aagctctagg tcgacggcct
     3661 ggaaaaaccc ttcgagcagc aggatgggct tcgctactcc ggcgctgcgt aagcgctccg
     3721 cctcgcgcaa gtcgagcacg gcgaagccgt cggccgagga cagcgcgcgc accgctcgct
     3781 cgacgccgtg accgtaggca ttcgccttga tcaccgccca tagcttcgcc gcacccgcgt
     3841 agcgcttcgc cacggtcaaa ttctgtgcga gcgcagccga ggaaatcgtc gcgcggatcg
     3901 gccggggcat gaaagggtat tgtgctataa agcgcgcggt ccaaaaacaa ggagcacgct
     3961 gggggaaagc cttcgattga actacggctt ctacatcatc atggcggcgc agtttttctc
     4021 gtcgctcgcc gacaatgcgc tgctggtggc ggcaatcgcg ctgctcatcc aggccgaggc
     4081 gccgtcttgg ctgaccccgt atctcaaatt cttctttgtc gtctcctacg tggtgctcgc
     4141 gccctatgtc ggcgccttcg ccgaccgcct gcccaagggg caggtgatgc tgatcgccaa
     4201 caccgtcaag atcatcggct gcctgaccat gctgtggggc tcgcatccgc tgatcgccta
     4261 cggcatcgtc gggctcggcg cggcggcgta ttcgccggcg aagtacggca tcctcaccga
     4321 atacctgccg catagaaagc tcgtggtggc gaacggctgg atcgagggcc ttaccgtggc
     4381 gtccatcatc ctcggcacca tgcttggcgg gctgctcatc cacgttaggg tctccaccga
     4441 gctgctgtcc tggaacctgc cgctcatcga cacctccatc gacaccccgg ccgaggcggc
     4501 gatcgcgatc atcatcgtgg tgtacgcggt tgcggcgctt ttcaatctgg aggtgccgca
     4561 gaccggcgtt gcgctgaaga agctgccttc gcacccgatc gacacgctga aggacttcgc
     4621 gcgctgcgtg aaccacctgt ggcgcgaccg cctcggccag atctcgctcg cggtgaccac
     4681 gcttttctgg ggcgcgggtg cgacgttgca gttcattgtc atcgagtggg cgcgcgccgc
     4741 gctcggcttc gacctctcca aggccgcgat cctgcagggc gtcaccgcga tcggcatcgc
     4801 ggtcggcgcg gtgctcgcat cgatcctggt gacgctcgac cgctcggtgc gcgtcatccc
     4861 gctcggcatc gcgatgggcc tgatcgtgct cgcgatgaat ctcgtgagcc aggtctggct
     4921 cgcaatctcg ctcatggtgc tgatcggcgg cctcgccggg ttcttcgtcg tgccgatgaa
     4981 cgcgctgctt cagcaccgtg gccaccacct ggtcggcgcc ggccgctcga tcgcggtgca
     5041 gaacttcaac gagaacgcct cgatcctggt gatgatcgcg ctctactcgc tgctcctcgg
     5101 ggtcggctac tcgatctatt cggtgatcgt ggtgttcggg ctcttcgtcg cgggcaccat
     5161 gacgctggtg cagctgtggt tctggcgcaa ccgccgcgtc cgccacgagg aagtcgatcg
     5221 cttgctcgcc ttcgcgcgcg ccgaggaccc gcacaagcct cgggaatagc tggcgcgcta
     5281 caggcgctcc atggtcttgc gcgcgaacac gaggtcttcc caagccttcg ccttgtcttc
     5341 cagcgtgcgc agcaggtagg ccgggtgata ggtaacgatg agcggcaccc cggcgtagcg
     5401 gtgtacccgg ccgcgcaggc tcgcgatcga ggcgtcgctc gcgagcagag tctgcgccgc
     5461 gaaacggcca agggcgatga tgagcttcgg ctgcaccagc ccgatttgct gcaacaggtg
     5521 cggggtgcac ttctcgacct cttccggcgc cgggttgcgg ttgcccggcg gccggcactt
     5581 gagcacgttg gcgatgtaca cgttgctgcg ccgcgaaagc ccgatcgcgg cgagcatgtt
     5641 gtcgagaagc ttccccgctt ggcctacgaa cgggtcgccg agccggtcct cttcggcgcc
     5701 gggcgcctcg ccgatcagca tccaatcggc gttctcgtcg cccacgccga ataccgtctg
     5761 tgtgcgcgtc ttgtgcaacc cgcactgcac gcacgtggac acggccgact tgagggggat
     5821 ccatgccggg gcaacctgct ttttcctcaa gcgccagagc ggcgcgagac ccatctcggc
     5881 gaggatcgcg gtgcgctcgc tcacgcgagc ggcagcgcga gcacgagcgc gtcctcgcgg
     5941 ccggtgtgcg cgggataata gccgcgccgc accgcaactt tgcgaaagcc gaaccgcgtg
     6001 tagagcgcct gggcgccctg gttgctgggc cgcacctcga ggaagaggct gcgggcgccg
     6061 atcttgcggg cgagcccggc ggcctgcgca agaagccatg cgccgtgccc ggcgcgctga
     6121 tgcgcggcgg cgatcgaaag gttgagcaga tggccctcgg ccgcggcgac catgagcacg
     6181 aaatagccga gaagctccgg cccgagccgc agcacccggc attcgtagcc tgcgcgcagc
     6241 gagtcggcga agttgccgcg cgtccagggg tgcgtgtaga tcgcgctctc caccgccatc
     6301 acctcgtcga ggtcgcgctc ggccatgggg ttcaggcgcg ggatatcctt aaggacggcg
     6361 ctcaagctct tccttcgtga aagcgacttt gtcgcgaaga tagaacggcg cggcctgcgc
     6421 tgcgtcaacg ccctcgccag cagcaagccg cggcgcggca agctgggcga ccgccatcgc
     6481 cgtcggatgg atttcgggcc tcgcgacttc gaccttggct ttcatttcct ctcggtaaac
     6541 ctcgaacccg cttccgcagc ccgtccagcc tttgccgggc ggcaacggcg cggagcgcgg
     6601 cgcgacgcaa tccgccggga tgatttcgtg ccagcgtccg tcgcgctttt cgagcgccgc
     6661 ataatacact tcgcgcatcc gcgcatcgat gcacgcaacc acgcgcgcgg cgccggattc
     6721 ctgtgccagt gcttcgagcg tcgagattcc gatcaccggc aggctgcgcg cgaaggcaag
     6781 gccctgcgcg agcgcgcagg cgatgcgcag cccggtgaat gcgcccggcc ccgcgccgaa
     6841 agccaccgcg tcgatccccg agcccttgat aagttcttga agcatcggca aagcgaattc
     6901 cgagtggcgt tggcccgcgc ggcgctcgag cgccgctatc tcgccgtcgt tccacaccgc
     6961 gaccgagcac cactccgtgg acgtctcgat cgccgcgaag cgcatgcgcc gattctatat
     7021 actgggacgc atgaaagaac actccattgc agtcatcccg ggcgacggca tcggcaggga
     7081 agtcatgccc gaaggagtac gcgtgctcga ggcggtgggc gcaaagcacg ggctgcgctt
     7141 caagtggcag gagttcccct ggagctgcga ctggtacaag gcgcacggga agatgtgccc
     7201 ggacgatgcg cccgacattc tgcggcgctt cgacgccatc tatttcggcg cggcgggcaa
     7261 tccggcgatt gtgcccgatc acgtctcggc gtgggggctg ctcatcaact tccgccgctg
     7321 gttcgacctg tatgtgaacc tgcggccggt gcggctgttc gaagggctcc cctgcccgct
     7381 cgccggccga aagcccggcg acatcgacta cgtggtgatc cgcgagaaca ccgaaggcga
     7441 atacggcaac gtggggggaa gggtttttga gaatacagaa aatgaaattg tgatgcagca
     7501 ggccgtgttc agccgcaagg gcatcgaccg catcatgaag tattcgttcg agctcgcgcg
     7561 cagccgcaag aagcatctca cgtcgtgcac caagtcgaac ggcatcacga ttaccatgcc
     7621 gtactgggac gagcgcttcg ccgagatgaa gaacaactac ccggacgtga aggccgacca
     7681 gtaccacgtc gacggtctca cgatccagat ggtgttgaac cccgatcgct tcgacgtgat
     7741 cgtggcttcg aacctcttcg gcgacatcct gtccgatctc ggtccggcga cggcgggcac
     7801 gatcgggatc gctgcgtccg gcaatatcaa cccggagcgc accgcgccgt cgatcttcga
     7861 gccggtgcac ggctcggcgc ccgatatcgc gggccggcgg atcgccaatc cgatcggtgc
     7921 gatctggtcg ggcgcgctga tgctcgaaca cctcgggcac ccgcgcgccg ccgccgacgt
     7981 gttgcgcgcc atagagcgcg cgctgatcga gggcccgcgc acgccggaca tgaagggcaa
     8041 ggcgagcact tccgaagtcg gcacggccat cgctgaagag atccggaccc ggtcgactct
     8101 cgccgcttga agacgtaccg catcgcgtcg atccccgggg acgggatcgg caacgaagtc
     8161 attcccgccg gaatggaagt cctcaaccgt gtagcggata gtttcgcgct cgagttccgg
     8221 catttcgact ggagctcgaa gcgctacgtc gaaaccggac gctacatccc cgagggcggg
     8281 ctggaagagc tgcgcaagtt cgacgccata ttcttcggcg cggtcggcgc cccggacgtg
     8341 cccgaccatg tctcgctctg gggcctgcgg ctgccgatct gccagggttt cgaccagtac
     8401 gccaatgtgc gaccggcgcg ggtgttaccc ggtgtgaaaa gcgttgttcc agatatcgac
     8461 tgggtcatcg tccgggaaaa caccgaaggt gagtactccg gctcgggagg ccgcgtgcac
     8521 cagaacctgc cagaggagat cgcgaccgag acctcggtct tcacgcgtaa aggcgtcgag
     8581 cgtgtgcacc gcttcgcgtt cgagctggct cgccagcgcc cgcgcaagca cctcactttg
     8641 gtgaccaagt caaacgcgca gcgtcacggc atggtcttgt gggacgagat tttttttgag
     8701 gttcaaaagc actatcccga ggtgcagacc gaccgcatcc tcgtcgacgc catgaccacc
     8761 cgcatggtgc tgaagcccgg ctccctcgac accgtcgtcg cgtcaaacct gcacgccgac
     8821 atcctttcgg accttgcagc agcgctctcg ggttcgctcg ggatcgcgcc caccgccaac
     8881 ctgaatcccg agcgcaa
//
LOCUS       DASTPD010000329         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_796182, whole genome shotgun sequence.
ACCESSION   DASTPD010000329 DASTPD010000000
VERSION     DASTPD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796182"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VFR57_10965"
     CDS             complement(<1..1338)
                     /locus_tag="VFR57_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU0259923.1"
                     /translation="MHRGQLFRKYFLLILALVCGALIISGGISLYFSYEENKEALASL
                     QREKAAGAAARIEQFVSQIEQQLAFAALPQLGAEGLEQRRIEFLKLLRVVPAVTDIAQ
                     IDPKGREQLAVSRLKMDTAGANEDRSQEPAFKNARPGQTWFGPVYFRKETEPYMSIAV
                     RSGGEQGPVTVAEVNLKFIWDVVTRIKIGQKGKAYVVDSTGHLVADPDIGLVLRKTDL
                     SALEQVKAAFAPGADESHAMVANDLNGKPVLTAYAPIEPAREQASRGAQFSPLGWKVF
                     VEQPASEVYAALDATIYRTVGLIVAGLLFSAVAALWLARNMVRPINVLQEGAQRIGEG
                     HLEQKIDIRTGDELEALASQFNRMTEQLRESYAGLERKVEERTAQLTESLAYQTATGE
                     ILQVISSSPTNVQPVADAVAERAAHLCHAPFARLMLADGPLLRPVAQYREGRLT"
     gene            complement(1415..1681)
                     /locus_tag="VFR57_10970"
                     /pseudo
     CDS             complement(1415..1681)
                     /locus_tag="VFR57_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            complement(1736..2710)
                     /locus_tag="VFR57_10975"
     CDS             complement(1736..2710)
                     /locus_tag="VFR57_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259924.1"
                     /translation="MNRRDFFLGALSVALAVKAQAQARHYRIGYLTSATPDAYNPMLD
                     VFIATLGRHGYQQGRNLSLVQRWSRSPGASAELEKHAVELTNLPVDAIVTWGTQATAA
                     AQKATRRIPIVMANTADPLGSRFVESLARPGGNITGISSLSTDLSGKVLSLLKEIVPG
                     ATRIGVFRNPANPVSKHHLQSAQRSAKTLDVELIVAEVHGADLPAAFAKLSAASVQGA
                     IALADPAFVSHREQFATLLLRHRLPVAFQRSENVEAGGLISYSADLREQFRLAAGYVH
                     RILQGAAPGDLPVLQPAKVELVANLKTARALGVTIPQAVRLRVDRVIE"
     gene            complement(2789..>3217)
                     /locus_tag="VFR57_10980"
     CDS             complement(2789..>3217)
                     /locus_tag="VFR57_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEU0259925.1"
                     /translation="ESASQSLRLELRLYPVRDADAIRKSFAAMQTDRVEALLLGNDVL
                     LISEAASIAEMATRQRIRSAGNREFAESGGLIGYGSILDVVRHAATYVHKILRGAKPA
                     ELPIEQPSKYEVYLNLRTVKAGDFAIPPSFRLLRVDRVIE"
BASE COUNT          514 a         1072 c         1062 g          569 t
ORIGIN      
        1 cgtcagccgg ccctcgcggt actgcgccac cggccgcagc agcggcccgt cggccagcat
       61 gagacgcgcg aacggcgcgt ggcacaggtg ggcggcccgc tcggccaccg cgtcggccac
      121 cggctgcacg ttcgtcggcg agctcgagat gacctgcagg atttcgccgg tggcggtctg
      181 gtaggcgagc gactccgtga gctgcgcggt gcgctcctcg accttgcgtt ccaaacccgc
      241 gtaggactcg cgcagctgct cggtcattcg gttgaactgc gaggcgagcg cctcgagctc
      301 gtcgcccgtg cggatgtcga tcttctgctc gaggtggccc tcgccaatgc gctgcgcgcc
      361 ttcctgcagc acgttgatcg ggcgcaccat gttgcgcgca agccagagcg cggcgacggc
      421 cgagaagaga aggcccgcga cgatcaggcc gacggtgcga tagatcgtgg cgtccagcgc
      481 cgcatagacc tcgctcgccg gctgctcgac gaacaccttc cagccgagcg ggctgaattg
      541 cgcgccgcgc gacgcctgct cgcgcgccgg ctcgatcggc gcgtaggccg tgagcaccgg
      601 cttgccgttc aggtcgttcg ccaccatggc gtgcgactcg tcggcgcccg gcgcgaacgc
      661 cgccttcacc tgctcgagcg ccgagagatc ggtcttgcgc agcacaagcc cgatgtcggg
      721 gtcggcgaca aggtggccgg tcgagtccac gacatacgcc ttgcccttct ggccgatctt
      781 gatgcgcgtc accacgtccc agatgaactt gagattcacc tcggcgacgg tgaccggacc
      841 ctgttcgccg cccgagcgga ccgcgattga catgtacggc tcggtttcct tgcggaagta
      901 caccggcccg aaccaggtct ggccgggacg cgcgttcttg aaagccggct cctgcgagcg
      961 gtcctcgttc gcgccggccg tgtccatctt caggcgcgag accgcgagct gctcgcgccc
     1021 cttggggtcg atctgcgcga tgtcggtcac cgccggaacc acgcgcaaga gcttcaggaa
     1081 ctcgatgcgc cgctgctcaa ggccctcggc gccgagctgc ggcagagcgg cgaaggcgag
     1141 ctgctgctcg atctgactca cgaactgctc gatgcgcgcg gcagcgcccg cggccttttc
     1201 gcgctgcaga ctcgcgagcg cttccttgtt ctcctcgtag gagaagtaca ggctgatgcc
     1261 gcccgaaatg atgagcgcgc cgcacaccag cgccagaatc agcaggaagt acttgcggaa
     1321 gagctgcccg cggtgcacgc gcttgggcgc ctcgaccgcg cggcgctgct cgacgatggg
     1381 ctcgaccagt ttcagcgcct gtcctcgttg cggatcactt tatcacctcg tccgccgtgt
     1441 acagcacgga cggcggaacc gcaatgccca gggctttcgc gctcttcgta ttgattacga
     1501 gttcgaactt caccggctgc tcaacgggca gctccgccgg gtttgccccc ttgaggatgc
     1561 ggtcgacgta gagcgccgtg cggcggtaat ggtcgatgcg gtcgggcccg agagagatca
     1621 atccgccctc ttcgacaaac tcgcggtagc cgtacattac gggcagccga agcttctccg
     1681 cccggctctt taggtttcag cccctcccct cctgcgctca ctctatcacc ggcgctcact
     1741 cgatgacgcg gtccacccgc agtcgtacag cctggggaat cgtcacaccc aacgcgcgcg
     1801 ccgtcttcag gttggcgacg agctcgactt ttgccggctg cagaaccgga aggtcgccag
     1861 gggcggcccc ctgcaggatg cggtgcacat aaccggcggc aaggcggaat tgctcgcgca
     1921 ggtccgcgct gtaggaaatg aggccaccgg cttcgacgtt ctcgctgcgc tgaaacgcca
     1981 cgggtaggcg atggcgaagc aggagcgtcg cgaactgctc gcggtggctg acgaaagccg
     2041 gatccgcgag tgcgatcgcc ccctggaccg acgctgcgct cagcttggcg aaggcggccg
     2101 gaaggtcggc gccgtgcacc tccgcaacga tcaactcgac gtcgagcgtc tttgcggacc
     2161 gctgggcgct ctgcaaatgg tgcttgctca ccgggttggc cggattccga aagacgccga
     2221 tgcgcgtcgc gccgggcacg atctccttga ggagtgacag caccttcccg ctaaggtccg
     2281 tcgacaggct actgataccg gtgatgttgc cgcccgggcg cgccaggctc tcgacaaacc
     2341 tcgaacccag cggatcggcg gtgttcgcca tgacgatggg gatgcggcgc gtcgcttttt
     2401 gcgcagccgc cgtggcctgc gttccccagg tcacgatggc gtccacgggc agattggtca
     2461 gctccacagc atgcttttcg agttcggcgc tcgcaccggg gctgcggctc cagcgttgca
     2521 ccaagctaag gttgcgtccc tgctggtatc cgtggcgccc aagggtggct atgaagacat
     2581 cgagcatggg gttgtaggca tcgggcgtcg cagaggtgag gtagccgatc cggtagtgac
     2641 gcgcctgtgc ttgggcctta accgccagag ctacggaaag cgcacccaga aagaaatcgc
     2701 gccgattcat cggcgtactg cgtcagcgcg cggcggaacc gaggacggca cagctatgtg
     2761 tcgaacccgc gagcggcgcc ttgccgcttc actcgatcac cctatccacg cgcagtagcc
     2821 ggaatgacgg tggaatggcg aagtcgcccg ctttcaccgt gcgcaggttg agatagacct
     2881 cgtacttgct cggttgctcg atcggaagct cggccggttt cgcgcccctc aggatcttgt
     2941 gcacgtaggt cgccgcgtgg cgcaccacgt ccaggatcga tccatagccg attaggccgc
     3001 cgctttcagc gaattcgcga ttgccggccg agcggatccg ttgccgcgtc gccatttccg
     3061 cgatggatgc cgcttcgctg atgagcagta cgtcgttgcc gagcaggagc gcttccaccc
     3121 ggtcagtctg catggcggcg aagctcttgc ggatcgcatc ggcgtcgcgt acggggtaga
     3181 ggcgcagctc gagtctcaga gactgggaag cgctctc
//
LOCUS       DASTPD010000330        15586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1408501, whole genome shotgun sequence.
ACCESSION   DASTPD010000330 DASTPD010000000
VERSION     DASTPD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408501"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFR57_10985"
     CDS             <1..323
                     /locus_tag="VFR57_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259926.1"
                     /translation="PRFVGDRIARQAILAERRIDCDSEAGRLICDEIVPAERMDEAIN
                     AMMDRLTSSGVVSAAGNRRAFRGGEEPLDTFRRYMAIYAREQAECHLSPALVCNLERN
                     WKQR"
     gene            320..763
                     /locus_tag="VFR57_10990"
     CDS             320..763
                     /locus_tag="VFR57_10990"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU0259927.1"
                     /translation="MKILVLNGPNLNLLGRREPHIYGSTTLAEILKKLEQLGKELGTE
                     IIPFQSNHEGELVDFLHKHIDSAQGALLNPAGLTQYGVSLHDAIKTMPFPVIEVHISN
                     RHAREHWRGHSIISPAVKATVEGLGWRSYTAALRALVEILKEKNS"
     gene            775..2661
                     /gene="iolC"
                     /locus_tag="VFR57_10995"
     CDS             775..2661
                     /gene="iolC"
                     /locus_tag="VFR57_10995"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEU0259928.1"
                     /translation="MCIGRCSVDLYGQQIGSRFEDIASFAKSVGGSPANMAIGTARLG
                     LKSGLITRVGDEQMGRFVLEQMRREGVALDGIKTDPQRLTALVLLAVEDEGVSPHIFY
                     RTDCADMALCEEDIDEAYIASARAVVVTGTHFSRPNSEAAQRKAMRIAKQNGAKVAFD
                     IDYRPTLWGLTGHAAGFERYVKSERVSERLRSVLSDCDLVVGTEEEIMIAAGADSVLA
                     ALKAIRSISKATIVLKRGPMGCVVYEREIEGLDNGIVGRGFPIEVYNVLGAGDAFMSG
                     FLRGWLKGEPLSTCATWANACGAFAVSRLLCSPEYPTWAELSHFLEKGSKERALRKDA
                     ALSHLHWATTRRTQPERLMALAIDHRTQLEALADRCGASRSRLEQFKLLALEATTRVA
                     RGRPEFGILLDSTYGHRALYKAAKTALWIGRPVEKPGSRPLDLEPFHLAEWPVTHTVK
                     CLCFYHPDDAEELKTRQERELVRVYDAARSTGRELLLEIIAGKHGVLAKDTVASALAR
                     LYALGIRPDWWKLEAQKSDAAWRAIEQVVKTNDPYCRGVVMLGLEAPEAELIEALRVA
                     SSAKIVKGFAVGRTIFASAAEQWLSGRMGDEAAVADMAAKFQNLVSAWEEVASVETSR
                     TAAR"
     gene            2672..3427
                     /gene="iolB"
                     /locus_tag="VFR57_11000"
     CDS             2672..3427
                     /gene="iolB"
                     /locus_tag="VFR57_11000"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU0259929.1"
                     /translation="MTSITPQSAGWKHVGFEVHKLAKGESAAAATGEREVCLVLVSGK
                     AKVKAGGKDFGWVGERMTPFEGKPHSVYVPGGSDWRVEATTEVTLAVCSAPSAGGNLP
                     ARHIKPQDMGQETRGKGTNVRYVTNILPEETPADSLLVVEVITPGGHTSSYPPHKHDT
                     DNLPNESSLEETYYHRVNPPQGFAFQRVYTDDRSIDEAIVVEDGSVVMVPRGYHPVAA
                     VHGYDLYYLNVMAGPRRTWKFHNAPEHEWVLKK"
     gene            complement(3432..3656)
                     /locus_tag="VFR57_11005"
     CDS             complement(3432..3656)
                     /locus_tag="VFR57_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259930.1"
                     /translation="MTRTLTTVAVLATLSGCASDCAGDWYAIGQRDGRLGATPQADIY
                     ASRCGGNVDRARYQEGWQSGDAMRPKTPYY"
     gene            3655..4206
                     /locus_tag="VFR57_11010"
     CDS             3655..4206
                     /locus_tag="VFR57_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0259931.1"
                     /translation="MRLANTSGLCLFLVVLLSATARADEEIWRRLQQGAHVVLIRHAV
                     TTPGAGDPPGMRLDDCSTQRNLTDEGRAHARRIGEEWKKRAVPVDRVLSSPWCRCLET
                     ARLAFGKAEVAEALSNLFGRPENRERQVRELRALVEKHAGKGNLVLVTHGSTIAALTG
                     VNPATGEMVILRDGALAGRLTVP"
     gene            complement(4203..4520)
                     /locus_tag="VFR57_11015"
     CDS             complement(4203..4520)
                     /locus_tag="VFR57_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0259932.1"
                     /translation="MISRTKEVSAARMVEDVVGCKWSLAVLGLVRQGVHRPGAMEHAI
                     PGLSKKVLNERLRKLVRFGVLQREAYAEVPPRVEYRLTPFGEKFAVLMDGVERLQRQL
                     DAD"
     gene            4600..5064
                     /locus_tag="VFR57_11020"
     CDS             4600..5064
                     /locus_tag="VFR57_11020"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEU0259933.1"
                     /translation="MRKLCAVLLFPTAALAADPVPYPEGYRNWTHVKSMVIEKGHPLY
                     EAFGGIHHLYANKQALEGYRAGKFPGGAVIVFDLLEAKSADNAVQEGARKVLGVMHRD
                     PKKWKDTGGWGFEGFKGDSKSERAVGSNAAAACFQCHTSQRHKDFVFSSYRK"
     gene            complement(5102..6049)
                     /locus_tag="VFR57_11025"
     CDS             complement(5102..6049)
                     /locus_tag="VFR57_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259934.1"
                     /translation="MRFLLAILLFAAVNAAAQTWPTRSVRIVVPATPGGAIDLIARTL
                     AEKLTTSLGQPVVVENKPGASNNLGTDFVAKSPPDGYTLVIVASSHATNKHLFKQMPF
                     DPIKDFEPVVYTHVVPLLLAVHPSVPAKNVPELVAWIKANPDKAVYASSGPGSSLHMA
                     AELFMNMTKTKMHHVPYKGSSAAHPDLLAGRTAMIFDTITAIRGHVQSGAVRGLAVTT
                     LKRSSAMPDLPAISESLPGYDASTWGGILAPAGTPKVAVSRLNSSINDALKMPDVRTR
                     LTGAGIEIQGGTPEAFAEVIRNEVEKWGRIVKEAGIPPE"
     gene            complement(6060..6788)
                     /locus_tag="VFR57_11030"
     CDS             complement(6060..6788)
                     /locus_tag="VFR57_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0259935.1"
                     /translation="MIEMIKVNKYFAAFHVLKDIDLLVSRGEKVVVCGPSGSGKSTLI
                     RLVNRLEEHQSGKVVVDGIELTDDVKKTDMVRREVGMVFQSFNLFPHLTVLQNLTLAP
                     IWVKRVSRKEAEDTAMHFLARVRIPEQAHKYPSQLSGGQQQRVAIARALCMKPKLMLF
                     DEPTSALDPEMIKEVLDVMVELAREGMTMMVVTHEMGFARTVADRVIFMDEGRIVEAA
                     PPADFFSAPKTDRAKLFLSQVLGH"
     gene            complement(6785..7801)
                     /locus_tag="VFR57_11035"
     CDS             complement(6785..7801)
                     /locus_tag="VFR57_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0259936.1"
                     /translation="MRWARENLFRSWPETIATLLVLWLLWKTIPPFIDWAFLDAVWRP
                     DSKACRASEGACWGFVAEKHRFILFGTYPYELHWRPALATVILIGLWVFSAFRAFWKS
                     WLVAVWAVGLAVIGSLMWGGVFGLRYVENERWGGLILTLLLTTFGIAAAFPLGILLAL
                     GRRSDMPVIRALSVGYIELIRGVPLISLLFMASVMLPLFLPSGVTIDKLLRAQIAIIL
                     FAAAYLAEVIRGGLQAIPKGQYDASHALALPFWRRTYLIILPQALRIAVPALVNNFIA
                     LFKDTSLVLIIGLFDLLSTVKISLNEPAWTGFGVEAYLIASLIYFVFCAAMSTYSKSF
                     EVRR"
     gene            complement(7798..8748)
                     /locus_tag="VFR57_11040"
     CDS             complement(7798..8748)
                     /locus_tag="VFR57_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560484.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0259937.1"
                     /translation="MVWRSARGKRIAYQAAAILAAVLVAWFLISNTMANLEERRIASG
                     FGFLGRESGFEIGETVFMRYSASDSYARALVVGLTNTLAVSIIGVGIASVLGTFIGLS
                     RLSHNFLLRNAAAAYVEFMRNVPLLVQLFFWYAVITEGMPGPREALSPFPGFFISNRG
                     IFFPTFSGVPELQGFNFTGGGAISPEFAALLIGLATYTAAFIAEIVRAGVLAVHRGQT
                     EAARALGLSRAQTTRLIVLPQALRVIVPPLTSQYLNLTKNSSLAVAIGYPDLVSIANT
                     TMNQTGQAIEGIAIIMAVYLTISLSISAFMNWYNARIAFR"
     gene            8747..9718
                     /locus_tag="VFR57_11045"
     CDS             8747..9718
                     /locus_tag="VFR57_11045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0259938.1"
                     /translation="MTERKLTRDDLRRAAEKYKNWGRWGPADEIGTLNHTRPEDIVAA
                     ARLVRKGKVISLALNFDNNGPQGAKSKYPALGRINPLHTMLRTGTDAYSGVLDKRGIR
                     AADDMVVMPLQCGTQWDGLGHVFYENNMWNGYDCREVTSAGAQKCGIEKTKNKMVGRG
                     VFLDVPRALGMDILPDGFAIKNAHLDETARKQKIEVRRGDFVVVRTGQMEAKLAAGSW
                     DGYPGGDAPGFSFETLEWIQRTELAALAADTWGCEVRPNETEAGINQPWHWITIPIMG
                     MTMGEIFYVKDLAEDCAEDKVYEFLFVAPALPITGAVGSPTNPLAIK"
     gene            9715..10662
                     /locus_tag="VFR57_11050"
     CDS             9715..10662
                     /locus_tag="VFR57_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259939.1"
                     /translation="MKTILALLLLAAGTAFGQPFPSKPIRMVVAFPPGGPVDVVARLL
                     SPKMSETLGQSIVIENVGGGGGNIAAQRVARSAPDGYTVLAHSSAYAVNPTLMGNLGF
                     DAEKDMTAIAVVASQANLIFVHADFPAKSLKELLERARTEKLAFASPSSGTTPHLTGE
                     NLFRLRAKVDITHIPFKGAGPAMTAVLGGQPPIGVLAGTAPLPHVKAGKIRVLAVSSA
                     KRLASLPDVPTLNELGFSGMEDYTWVGLFVPAGVPAEVAQKLNAALLAAVRSPEVKER
                     LEALAFEITAAPLAETATYVKSELAKWGKVVRETGAKVD"
     gene            complement(10649..11125)
                     /locus_tag="VFR57_11055"
     CDS             complement(10649..11125)
                     /locus_tag="VFR57_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0259940.1"
                     /translation="MKRWIATFGLVAAFAHANPAIDMPGYLEVAREAAQHRQARRVSE
                     EEFMRMSREPGTVVLDARSRDKYDELHVNGAINLPFPDIAIESLKATIPDKDTRILIY
                     CNNNFANAEGAFPAKIARASLNLSTYIALYSYGYRNVYELGPLVELRQSKLEFSRP"
     gene            complement(11178..12428)
                     /gene="egtB"
                     /locus_tag="VFR57_11060"
     CDS             complement(11178..12428)
                     /gene="egtB"
                     /locus_tag="VFR57_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEU0259941.1"
                     /translation="MKLRRTHASPRPALKDDYRNVRQTSLALAAPLSAEDCAIQSMPD
                     ASPVKWHLAHTTWFFETFVLVPHVPGYRPFDPAFGYLFNSYYNAIGERHPRPERGMLS
                     RPSLDGVRAYREHVDAAMGAASNVPHALVELGLHHEQQHQELILTDLKHMLSRNPLRP
                     AYQKQWPLTPIRARAPNWVGFAGGLHEIGHGGAGFAFDNETPRHKVWLEDYEIASHPV
                     THGDFLDFIADGGYRRPQLWLSAGWDLVRARGWEAPEYWTRRDGTWHTFTLHGEVRVD
                     RNTPICHVSFFEAEAYARWAGARLPTEAEWEVAARSTPLGGNFLESGALHPLALREDP
                     PVGELAQIYGDVWEWTRSDYAPYPRYRPAEGAVGEYNGKFMCAQYVLRGGSCATPASH
                     IRATYRNFFPPEARWQFSGLRLAK"
     gene            complement(12463..12795)
                     /locus_tag="VFR57_11065"
     CDS             complement(12463..12795)
                     /locus_tag="VFR57_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0259942.1"
                     /translation="MEYYFEEGCFITELSNTPEHPDLSIARARVPVGVTTRWHRVKDT
                     AERYVILDGRGRAEVGEQPPREVGPGDVVVIAASQRQRIANIGDKDLVFLAICTPRFE
                     RAAYEDLE"
     gene            complement(12798..13181)
                     /locus_tag="VFR57_11070"
     CDS             complement(12798..13181)
                     /locus_tag="VFR57_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0259943.1"
                     /translation="MLERNKKNVVAFYDLMFNQSKPREAMERFAGDTYIQHNPGVADG
                     KEAFIDYFERMARDYPGKRVHFKRVVAEGNYVVLHCHQEWQGYKDKDWAGIDIFRVDD
                     SGKIVEHWDVLQIVPPQSANANTMF"
     gene            complement(13184..13597)
                     /locus_tag="VFR57_11075"
     CDS             complement(13184..13597)
                     /locus_tag="VFR57_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0259944.1"
                     /translation="MHFHRGRLVDHVHLRVADLTVSKRFYRAVLESLGRAQGIREGDD
                     FFNSDELWIDKADGPVSRVHLAFQAESPAQVRAFHAAALRAGGRDNGAPGERPYHPGY
                     YAAFVFDPDGNNIEAVHHGPAKRSAESIVITPTGG"
     gene            complement(13598..14386)
                     /locus_tag="VFR57_11080"
     CDS             complement(13598..14386)
                     /locus_tag="VFR57_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0259945.1"
                     /translation="MNQKEKVQRFRAMHGSAFVIANAWDAGSARILAGLGFQALATSS
                     GAHAGTLGKRDGMVTREEALEHARAIVEATELPVSADFERCFADDPQEAAETVRLAAE
                     IGLAGCSIEDATKDRVYDFEHAVERVAAAARVKKLVLTARTENFVRSRPDLDDTIRRL
                     QAFEKAGADVLMAPGLPDLQSVRKVCASLSKPFNFMAGIKGKSFSVAELEAAGVKRVS
                     LATSLWRAAMSGFMEAAREVKEKGTFGYVERSVPTPEIAKFMKS"
     gene            complement(14383..15351)
                     /locus_tag="VFR57_11085"
     CDS             complement(14383..15351)
                     /locus_tag="VFR57_11085"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0259946.1"
                     /translation="MTRRAVLASGLLFFAAPLSGVAQQPRLYRIGVLLYGGVYQAALE
                     GLRERLAQLGLEEGKHFVFHQREARGDLKSVESTASALEREQVDVIFTVTTSVTAAAK
                     RGTKTVPIVFYAGNDPVAMGFVDSFHRPGGRLTGVHSRFTNLAAKRLELLKEMIPGLR
                     RVVTFYRPDNPANTEVIKTTRDAARRLNVEVAERYIASVDELRASLEALRPGDADAFY
                     SADSMVISQAQLVIDILNPKKVATIFNDRDLVMKGGLASYGASYHALGGLAAGQVERI
                     LRGAKPGDLPVEQLDRLYLAVNLKTANALDLKIPQSVLARADEVIR"
     gene            complement(15359..>15586)
                     /locus_tag="VFR57_11090"
     CDS             complement(15359..>15586)
                     /locus_tag="VFR57_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0259947.1"
                     /translation="DKESSSTNPTIQRFVGASGDIGKMLGIDNKWSFNIIKQVGNYGE
                     SFDNNLKPLGFERGLNRLWNQGGLMYAPPIR"
BASE COUNT         2826 a         5022 c         5132 g         2606 t
ORIGIN      
        1 tgccgcgctt cgtcggcgac cgcatcgcgc ggcaagcgat cctcgcggag cggcgcatcg
       61 attgcgacag cgaggcgggt cgcctcatct gcgacgaaat cgtgccggcg gagcgcatgg
      121 acgaagcgat caatgccatg atggacaggc tgacttcgtc gggcgtagtc agtgcggcgg
      181 gaaaccggcg cgcattccgc ggcggcgagg agccgcttga cacgttccgc cgctacatgg
      241 cgatctatgc gcgcgaacag gccgagtgcc acctgagccc cgcgctcgtc tgcaatctcg
      301 agagaaactg gaaacagcga tgaagatcct cgtcctcaac ggcccgaatc tgaacctgct
      361 cggccggcgc gagccgcata tctacggctc caccacgctc gccgagatac tgaagaagct
      421 cgagcagctc ggcaaggaac tcggcaccga gatcatcccc ttccagtcga atcacgaagg
      481 cgagctcgtc gactttctcc acaagcacat cgactcggcg cagggcgcat tgctgaatcc
      541 ggccggcctc acgcaatacg gcgtgtcgct gcacgatgcc atcaaaacca tgccgttccc
      601 ggtgatcgaa gtgcatatca gcaaccgcca cgcgcgggag cactggcgcg gccactcgat
      661 catctcccct gctgtgaaag ccacagtcga gggcctcggc tggcgctcct acaccgcggc
      721 gctgcgcgca ctggtcgaga tcctgaagga gaaaaactcc tgaagctcga cgtcatttgc
      781 atcgggcgct gctcggtcga cctgtacggc cagcagatcg gctcgcgctt cgaggacatc
      841 gcgagcttcg cgaagtcggt cggcggctcg ccggctaaca tggcgatcgg tactgcgcgc
      901 cttggcctca agtcgggcct gatcacgcgc gtgggggacg agcagatggg ccgcttcgtg
      961 ctcgagcaga tgcgccgcga aggcgtcgcg ctcgacggca tcaaaaccga tccgcagcgg
     1021 cttaccgcgc tggtgctgct cgccgtcgag gacgagggcg tatcgccgca catcttctac
     1081 cgcaccgatt gcgccgacat ggcgctttgc gaggaggaca tcgacgaggc ctacatcgcc
     1141 tcggcgcgcg ccgtggtcgt caccggaacg cacttctcgc gcccgaacag cgaagcggcg
     1201 cagcgcaagg cgatgcgcat tgcgaagcag aacggcgcca aggtggcctt cgatatcgac
     1261 taccgtccta cgctctgggg cctcaccggc cacgccgcgg gtttcgagcg ctacgtgaag
     1321 tccgagcgcg tgtccgagcg tctgcgcagc gtgctgtcgg actgcgacct ggtcgtcggc
     1381 accgaagaag agatcatgat cgcggccggc gcggacagcg tgctcgcggc attgaaagcg
     1441 atccgttcaa tttcgaaggc gaccatcgtt ctcaagcgcg gtccgatggg ctgcgtggtc
     1501 tacgagcgcg agatcgaagg gctggacaac ggcatcgtcg gtcgcggctt tccgatcgag
     1561 gtctacaacg tgctcggcgc cggcgacgcc ttcatgtcgg gcttcctgcg cggctggctg
     1621 aaaggcgagc cgctctcaac gtgcgcgact tgggcgaacg cctgcggcgc atttgcagtg
     1681 tcgcgtctgc tgtgctctcc cgagtatccg acctgggccg agctcagcca ttttctggag
     1741 aagggcagca aggagcgcgc gctgcgcaag gacgcggcgc tcagccacct gcactgggcg
     1801 acgacgcgtc gcacgcagcc ggagcgcctc atggcgctcg cgatcgacca ccgcacgcaa
     1861 ctagaggcgc tcgccgaccg atgcggcgcg tcgcgcagcc ggctcgagca attcaagctt
     1921 cttgcgctgg aggcgacgac gcgagtggcg cgcggccgac cggagtttgg catcctgctc
     1981 gactccactt atggacacag ggcgctctac aaggcggcga agaccgccct gtggatcggc
     2041 cggccggtgg agaagcccgg ttcgcggccg ctcgacctcg agccgttcca tctcgcggaa
     2101 tggccggtga cgcacaccgt taagtgcctg tgcttctacc atccggacga cgccgaggag
     2161 ctaaaaacac ggcaggaacg cgagctcgtg cgcgtctacg acgccgcgcg tagcaccggg
     2221 cgcgagctgc tcctcgagat catcgccgga aagcacggcg tgcttgcgaa agacacggtg
     2281 gcctcggcgc tcgcgcggct ttacgcgctc ggcatccgac ccgactggtg gaagctcgag
     2341 gcgcaaaaga gcgacgcggc atggcgcgcc atcgagcagg tggtgaagac gaatgatccc
     2401 tattgccgcg gcgtggtcat gctcggcctc gaagcgccgg aggccgagtt gatcgaggcc
     2461 ttgcgggtcg cctcgagcgc aaagatcgtc aagggattcg ccgtcggccg cactatcttc
     2521 gccagcgcgg cggaacagtg gctgtcgggg cgcatgggcg acgaagcggc agtcgctgac
     2581 atggcggcga agttccagaa cctggtttct gcatgggaag aggtggcgag tgtcgagact
     2641 tcacgtacgg ccgcgcggtg aacacggaaa agtgaccagc attaccccgc agagcgcggg
     2701 gtggaagcat gtcggcttcg aggtccacaa gctcgccaag ggcgaaagcg ctgcggctgc
     2761 gaccggcgag cgtgaggtgt gcctcgttct cgtgtcgggc aaggcgaagg tcaaggcggg
     2821 cggcaaggat ttcgggtggg tcggcgagcg catgacgcct ttcgaaggca agccgcactc
     2881 cgtgtacgtg ccgggcggca gcgactggcg tgtggaagcg actacggaag tgacattggc
     2941 cgtctgctcg gcgccctcgg ccggcggcaa tctgcccgcg cggcacatca aaccgcagga
     3001 catgggacag gagacgcgcg gcaagggaac gaacgtgcgc tacgtcacca atatcctgcc
     3061 cgaggaaacg cctgccgact cgctgctcgt ggtcgaagtg atcacgccgg gcggccatac
     3121 ctcgagctat ccgccgcaca agcacgacac cgataatttg ccgaacgagt cttcactgga
     3181 ggagacgtac taccaccgcg tcaatccgcc gcagggtttc gcgttccaac gcgtgtacac
     3241 cgacgatcgc tcgatcgacg aggcgatcgt ggtcgaggac ggcagcgtgg tcatggtgcc
     3301 gcgcggctac caccccgtgg ccgcggtgca cggctacgac ctttattacc tcaacgtgat
     3361 ggctggcccg cggcgcacct ggaaattcca caacgcgcca gagcacgagt gggtgctcaa
     3421 gaagtgaagc gctagtagta cggcgtcttc ggtcgcatgg cgtcgcccga ttgccaacct
     3481 tcctgatagc gcgcccggtc gacgttgccc ccgcagcggc ttgcgtagat gtcagcctgc
     3541 ggggtagcgc cgagccggcc gtcgcgctgg ccaatcgcat accagtcgcc ggcgcagtcc
     3601 gacgcgcagc cactgagcgt tgcaaggacc gcgacagttg ttagggtcct ggtcatgcgc
     3661 ctagcgaaca catccggcct ctgcctattc ctcgtcgtgc tgctgtccgc gaccgcgcgg
     3721 gcggacgaag agatatggcg acgactccag cagggcgctc acgtggtgct cattcgccac
     3781 gccgtcacca cgccgggcgc gggagacccg ccgggcatgc ggctcgatga ctgcagcacg
     3841 cagcgcaacc tgacggacga gggccgcgcg catgcgcggc gcatcggcga ggaatggaag
     3901 aagcgcgccg tacccgtgga tcgcgtgcta tccagcccct ggtgccgctg cctcgagacc
     3961 gcgcgcctcg ccttcgggaa agccgaggtc gcggaggcgc ttagcaatct tttcggacgg
     4021 cccgagaacc gggaacggca ggtgcgcgag ctgcgggcgc tcgtggagaa gcacgccgga
     4081 aagggcaacc tggttctcgt cacgcacggc tcgaccatcg ctgcgctgac cggcgtcaat
     4141 ccggcgactg gcgagatggt gatcctccgc gacggcgctc tcgccgggcg gctaactgtt
     4201 ccctagtcgg cgtcgagctg gcgctggagg cgttccacgc cgtccatgag cacggcgaac
     4261 ttctcgccga acggcgtcag gcggtattcg acgcgcggcg gcacttcggc ataagcctcg
     4321 cgctggagca cgccgaagcg caccagcttt ctcaggcgct cgttcagcac tttcttggac
     4381 aggcccggaa tcgcgtgctc catcgcgccg ggccggtgca cgccctggcg cacgaggccg
     4441 agcacggcga gcgaccattt gcacccgacg acgtcctcga ccatgcgcgc ggcggaaact
     4501 tctttcgtgc gagaaatcat gaagagatac cgaaaagtgc ccggggcacc gtttcgtgcc
     4561 tgctttcgcg gccccggcgc gctcgcaaca atagcaagca tgagaaaact ttgcgccgtc
     4621 cttctcttcc caaccgcggc gctcgccgcc gaccccgtcc cctatcccga gggctatcgc
     4681 aactggacgc acgtcaaaag catggtgatc gaaaagggtc acccgctcta cgaggcgttc
     4741 ggcggcattc atcacctcta cgccaacaag caggcgctcg agggctaccg tgcgggcaaa
     4801 ttcccaggcg gcgccgtgat cgtgttcgac ctgctggaag caaagagcgc cgacaacgca
     4861 gtgcaggaag gcgcccgcaa ggtcctaggc gtgatgcaca gggacccgaa gaagtggaag
     4921 gacaccggcg gctggggctt cgaaggcttc aagggcgatt cgaaaagcga gcgcgccgtc
     4981 ggatcgaatg ccgctgccgc gtgctttcag tgccacacct cgcagcggca taaggatttc
     5041 gtttttagca gctaccgcaa gtaaccccgg ccctttttcc agaaaaaggg tcctgacccc
     5101 attactcggg cgggatgccg gcctccttca cgatccggcc ccatttctcg acctcgttgc
     5161 gaatcacctc cgcgaacgcc tcaggcgtgc ccccctggat ctcgatgccg gcgccggtga
     5221 ggcgcgtgcg gacgtcgggc atcttgagcg cgtcgttgat ggatgaattg aggcgggaca
     5281 ccgcgacctt gggcgtgccg gccggcgcga ggatgccgcc ccaggtgctg gcgtcgtagc
     5341 caggcaggct ctccgaaatc gcgggcagat ccggcatcgc gctcgagcgc ttgagcgtcg
     5401 tgaccgcgag gccgcgcacc gcgccggatt gcacgtggcc gcggatcgcg gtgatggtgt
     5461 cgaagatcat cgcggtgcgc cccgcgagaa ggtcgggatg ggccgccgag cttcccttgt
     5521 agggcacgtg gtgcatcttg gtcttcgtca tgttcatgaa gagctcggcc gccatgtgaa
     5581 gcgagctgcc ggggccgctc gaggcgtaca ccgccttgtc ggggttcgcc ttgatccatg
     5641 caaccagctc cggaacattc ttcgccggca cggaggggtg cacggcgaga agcagcggca
     5701 ccacgtgcgt gtacaccacc ggctcaaagt ccttgatcgg gtcgaacggc atctgcttga
     5761 agagatgctt gttggtggcg tggctcgagg cgacgatcac cagcgtgtag ccgtcgggcg
     5821 ggcttttcgc gacgaagtcg gtgccgaggt tattggaagc gccgggcttg ttctcgacca
     5881 ccacgggctg gccgagcgaa gtcgtgagct tttcggcgag cgtgcgcgcg atgaggtcga
     5941 tggctccacc cggcgtcgcc ggcaccacga tccggaccga tcgcgtcggc caggtctgcg
     6001 cggcagcgtt gacggcggca aagaggagta tcgcgagcaa gaaccgcatt ggcatctcct
     6061 taatggccca gcacttgaga gaggaacagt ttcgcgcgat cggtcttcgg cgcgctgaag
     6121 aaatccgccg gcggcgcggc ctcgacgatg cgcccttcgt ccatgaagat cacgcggtcg
     6181 gcgacggtgc gcgcgaagcc catctcgtgc gtcaccacca tcatggtcat tccctcgcgc
     6241 gcgagctcga ccatcacgtc gagcacttcc ttgatcatct cggggtcgag cgcggaggtc
     6301 ggctcgtcga agagcatcag cttcggcttc atgcagagcg cgcgcgcgat cgcgacgcgc
     6361 tgctgctggc cgccggagag ctgcgaagga tatttgtgcg cctgctcggg gatgcgcaca
     6421 cgcgcgagga aatgcatcgc cgtgtcctcg gcctctttcc tggaaacgcg tttcacccag
     6481 atcggcgcga gcgtcaggtt ctgcagcacc gtgaggtgcg ggaagaggtt gaagctctgg
     6541 aacaccatgc cgacctcgcg ccgcaccatg tcggtcttct tcacgtcgtc ggtaagctcg
     6601 atgccgtcga ccacgacctt tccgctctga tgctcctcca gccggttcac caggcgaatg
     6661 agcgtggact tgccggagcc ggatggcccg cacaccacga ccttctcgcc gcgcgacacc
     6721 aggaggtcga tgtccttcag cacatggaac gcggcgaagt acttgttcac cttgatcatc
     6781 tcgatcatcg gcgcacctcg aagctcttgc tgtaggtgga catcgccgcg cagaacacga
     6841 agtagatgag cgacgcgatg aggtatgcct cgacgccgaa tccggtccag gccggctcgt
     6901 tcagcgagat cttcacggtc gacagcaggt cgaacaggcc gatgatgagc acgagcgagg
     6961 tgtccttgaa gagcgcgatg aagttattga cgagcgcggg caccgcgatg cgcagcgcct
     7021 gcgggaggat gatgagatac gtccttcgcc agaaaggcag cgcgagcgca tgggaggcgt
     7081 cgtactggcc tttcggtatg gcctgcagcc cgccgcggat gacctcggcc aggtacgcgg
     7141 cggcgaacag gatgatggcg atctgcgcgc gcaggagctt gtcgatcgtc acgcccgaag
     7201 gcaggaagag cggcagcatc accgaagcca tgaagaggag cgaaatgagc ggcacgccgc
     7261 ggatgagctc gatgtagccg acggacagcg cgcggatcac cggcatgtcg gagcggcggc
     7321 cgagcgcgag caggatgccg agcggaaagg cggccgcgat gccgaaggtc gtgaggagca
     7381 gcgtgaggat gaggccgccc cagcgctcgt tctcgacata gcgcaggccg aataccccac
     7441 cccacatcag actgccgatc accgccaaac ccaccgccca gacggccacc agccaggact
     7501 tccagaaggc gcggaaggcg gagaagaccc acagtccgat gagaatcacg gtggcgagcg
     7561 cgggccgcca gtgcagctcg tacggatacg tgccgaagag aatgaagcgg tgcttctcgg
     7621 cgacgaagcc ccagcacgcg ccctcggaag cgcggcaggc cttggaatcc ggcctccaca
     7681 ccgcatcgag gaacgcccag tcgatgaagg gcggaatcgt cttccacaag agccacagga
     7741 cgaggagcgt ggcgatcgtc tccggccacg agcgaaacag gttctctcgc gcccacctca
     7801 tctgaaggca atcctcgcgt tgtaccagtt catgaacgcc gagatcgaca ggctgatggt
     7861 gaggtacacc gccatgatga tcgcgatgcc ttcgatcgcc tgcccggtct ggttcatcgt
     7921 ggtgttcgcg atcgagacga ggtctggata gccgatcgcc acggcgagcg agctgttctt
     7981 ggtgaggttg aggtactggc tggtgagcgg cggcacgatg acgcgcagcg cctgcggcag
     8041 gacgatgagg cgcgtcgtct gcgcgcgcga gaggccgagc gcgcgcgccg cttccgtctg
     8101 cccgcgatgc accgcgagca ccccggcgcg cacgatctcg gcgatgaacg cggcggtata
     8161 ggtcgcgagt ccgatgagca gcgccgcgaa ctcgggactg atcgcgccgc cgccggtgaa
     8221 attgaagccc tgcagctcgg gcacgcccga aaaggtcgga aagaagatgc cgcggttcga
     8281 gatgaaaaag cccgggaaag gcgaaagcgc ctcgcgcggg ccgggcatgc cctcggtgat
     8341 caccgcgtac cagaagaaga gctgcacgag gagcggcacg ttgcgcatga actccacgta
     8401 cgccgcggcg gcgttgcgca ggaggaagtt gtgcgacagc ctggaaaggc cgatgaaggt
     8461 gccgagcacg ctcgcgatgc cgacaccgat gatcgacacc gcgagcgtat tggtcaggcc
     8521 gacgaccagc gcgcgcgcat agctgtcgga agcgctgtag cgcatgaaca ccgtctcgcc
     8581 gatctcgaag cccgactcgc gcccgaggaa gccgaagccg ctcgcgatgc ggcgctcctc
     8641 caggttggcc atggtgttcg aaatgagaaa ccacgccacc agcaccgcgg ccaggatcgc
     8701 cgctgcctga tacgctatcc gcttccccct cgcagagcgc cagaccatga ccgagcgcaa
     8761 gcttacccga gacgacctta ggagagcggc cgagaagtac aagaactggg gccgctgggg
     8821 acccgccgac gagatcggca cgctgaacca cacgcgcccg gaagacatcg tcgccgcggc
     8881 gcgcctggtg cgcaagggca aggtcatctc gctcgcgctc aacttcgaca acaacggccc
     8941 gcagggcgcg aagagcaagt acccggcgct cggacgcatc aacccgctcc atacgatgct
     9001 gcgcaccggc accgacgcgt attcgggggt gctcgacaag cgcggcatcc gcgcggcgga
     9061 cgacatggtg gtgatgccgc tgcagtgcgg aacgcagtgg gacggcctcg gccatgtgtt
     9121 ctacgagaac aacatgtgga acggctacga ctgccgcgaa gtcacctcgg ccggcgcgca
     9181 gaaatgcggc atcgagaaga cgaagaacaa gatggtcggc cgcggcgtat tcctcgacgt
     9241 gccgcgcgcc ctcggaatgg acatcttgcc ggacggcttc gccatcaaaa acgcgcatct
     9301 ggatgaaacg gcaaggaagc aaaaaatcga ggtgcggcgc ggggacttcg tcgtcgtgcg
     9361 caccggccag atggaggcga agctcgcggc gggaagctgg gacggctatc ccggcggtga
     9421 cgcgccgggc ttctcgttcg agacgctgga atggatccag cgcaccgagc tcgcggcgct
     9481 cgcagccgac acatggggct gcgaggtgcg tcccaacgag accgaagccg gcatcaacca
     9541 gccctggcac tggatcacaa tcccgatcat ggggatgacc atgggcgaga tcttctatgt
     9601 gaaggacctc gccgaggatt gcgctgagga caaggtgtac gagttcctat tcgtcgcccc
     9661 tgccctgccg atcaccggtg cggtaggctc tccaaccaac ccgctggcga tcaaatgaag
     9721 acgatcctcg ctctgcttct tcttgcggcc gggacggcct tcggccagcc ctttccctcc
     9781 aagccgatcc gcatggtcgt cgccttcccg cccggcgggc ccgtggacgt ggttgcgcgc
     9841 cttctctcgc ccaagatgag cgagacgctg ggtcaatcga tcgtgatcga gaacgtcggc
     9901 ggtggcggcg gcaacatcgc ggcacagcgc gtggcgagat ccgcacccga tggctacacg
     9961 gtgcttgcgc actcttcggc ctacgcggtg aatcccacgc ttatgggcaa tctcggcttc
    10021 gacgccgaga aggacatgac cgcaatcgcc gtggtggcct cgcaggcgaa tctcatcttc
    10081 gtgcacgcgg acttcccggc gaaaagcctc aaggaattgc tcgaaagagc gaggaccgag
    10141 aagctcgcct tcgcctctcc gtcgagcggg accacgccgc atctcaccgg cgagaatctg
    10201 ttcaggctgc gcgcgaaggt cgacatcacg cacattccgt tcaagggcgc cggacccgcc
    10261 atgaccgcgg tgctcggcgg ccagccgccg atcggcgtcc tcgctggcac cgcgccgctg
    10321 ccgcacgtga aggccggaaa gatccgcgtg ctagcggtgt cgtcagcaaa gcgactcgcc
    10381 tcgctgcccg acgtgccgac gctgaacgag ctcggctttt ctggaatgga ggactacacg
    10441 tgggtgggcc ttttcgtgcc cgccggggtt cccgccgaag tcgcgcagaa gctgaatgcc
    10501 gcgctgctcg ccgccgtgag aagccccgaa gtgaaggagc gcctggaagc cctcgcgttc
    10561 gagatcaccg ccgcgccgct cgccgagacg gcgacctacg tgaagagcga gctcgcgaag
    10621 tggggcaagg ttgtgcgcga gaccggggct aaggtcgact gaattcgagc ttggattgcc
    10681 gcagttcgac gagcggcccg agctcgtaaa cgttgcggta gccgtagctg tagagcgcga
    10741 tgtaggtcga aagattgaga gatgcgcgcg cgatcttcgc cggaaacgcg ccctcggcat
    10801 ttgcaaagtt gttgttgcag tagatgagga tgcgcgtgtc cttgtcgggg atcgttgcct
    10861 tgaggctttc gatcgcgatg tccggaaacg gcaggttgat cgccccgttc acatggagct
    10921 cgtcgtattt gtcgcggctg cgcgcgtcca gcacgaccgt gccgggctcg cggctcatgc
    10981 gcatgaactc ctcttcggag acgcggcgcg cctggcggtg ctgcgccgcc tcgcgcgcca
    11041 cctcgagata tcccggcatg tcgatggccg ggttcgcatg cgcaaacgcc gcgacgaggc
    11101 cgaacgttgc gatccaccgt ttcatggcgc cagtctcgca ccggcgcgaa tgatgagctg
    11161 cgcgaaactg taacgagtta tttcgcgagc cgcaggcctg agaactgcca gcgcgcctcg
    11221 ggcgggaaga agttgcggta ggtggcccgg atgtggctcg ccggcgtcgc gcacgagccg
    11281 ccgcgcaaga cgtactgcgc gcacatgaac ttgccgttgt actcgcccac cgcgccttcc
    11341 gcgggccggt aacggggata gggcgcgtag tcgctgcgcg tccattccca cacgtcgccg
    11401 tagatttgcg caagctcgcc caccggcggg tcttcgcgca gcgcgagcgg gtggagcgcg
    11461 ccactttcga ggaagttgcc gccgagcggc gtgctgcgtg ccgccacttc ccattccgct
    11521 tcggtcggca agcgcgcgcc ggcccagcgc gcataggcct cggcttcgaa gaagctcaca
    11581 tggcagatcg gcgtattgcg atcgacccgg acctcgccgt gcagcgtgaa cgtgtgccac
    11641 gttccatcac gacgcgtcca gtactccggc gcctcccagc cgcgcgcgcg cacgaggtcc
    11701 catccggccg aaagccacag ctgcggccgc cggtaaccgc cgtcggcgat gaagtcgagg
    11761 aaatcgccgt gcgtgaccgg atgcgacgcg atctcgtaat cctcgagcca gaccttgtgg
    11821 cgcggcgtct cgttgtcgaa cgcgaatccg gcgccgccgt ggccaatctc gtgcaggccg
    11881 cccgcaaagc cgacccaatt tggcgcgcgc gcacggatcg gcgtgagcgg ccattgcttc
    11941 tgatacgcgg gcctgagcgg attgcgcgac agcatgtgct tgaggtcggt gagaatgagc
    12001 tcctggtgct gctgctcgtg atgcagcccg agctccacca gagcgtgcgg cacattgctc
    12061 gcggcgccca ttgccgcatc gacgtgctcg cgataggcac gcactccgtc gagcgaaggc
    12121 cgcgacagca tgccgcgctc cggtcgtgga tgccgctcgc cgatcgcgtt gtaataggaa
    12181 ttgaagaggt agccgaacgc cggatcaaag ggccggtaac ccggcacgtg cggcactaga
    12241 acgaaggtct cgaagaacca ggtggtgtgc gcgagatgcc acttcaccgg actcgcgtcc
    12301 ggcatcgact ggatcgcgca atcctcggcc gacagcggcg ccgcgagcgc gaggctcgtc
    12361 tggcgcacgt tccggtagtc gtctttgagc gccggccggg ggctcgcgtg tgttcgccgc
    12421 agcttcacgt acgctggacc tccggcgcgg ccaaacgttc gcctactcga gatcctcgta
    12481 cgccgcgcgc tcgaaacggg gcgtacaaat ggcgagaaaa accagatcct tgtcgccgat
    12541 attcgctatg cgctgccgct gcgacgccgc aatgaccacc acgtcgcccg ggcctacttc
    12601 gcgcggcggc tgctcgccca cttccgcccg tccgcgcccg tcgaggatca cgtagcgctc
    12661 ggcggtgtcc ttaacgcgat gccagcgcgt cgtcacgccg accggcacgc gggcgcgggc
    12721 aatggagaga tccggatgct ccggcgtgtt ggaaagctcg gtgatgaaac agccttcctc
    12781 gaagtaatac tccacgctca gaacatcgta ttggcgttgg ccgactgcgg cggaacgatc
    12841 tgcaggacat cccagtgctc gacgatcttt ccgctgtcgt cgacgcggaa gatgtcaata
    12901 ccggcccagt ccttgtcttt gtagccctgc cactcctggt ggcaatgcag gaccacgtag
    12961 tttccttccg cgacgacgcg cttgaagtgc acgcgctttc cgggatagtc gcgagccatg
    13021 cgttcgaagt aatcgatgaa agcttccttg ccgtccgcga cgccggggtt gtgctgaatg
    13081 tacgtatcgc cggcaaatcg ctccatcgcc tcgcgcggtt tgctctggtt aaacatgagg
    13141 tcataaaagg ccaccacgtt cttcttgttg cgctcgagca tgttcatccg cctgttggcg
    13201 ttatgacaat cgactctgcc gacctcttcg ctgggccgtg atgcaccgcc tcgatgttgt
    13261 taccgtcggg atcgaagacg aaagcggcgt aatagcccgg gtggtaggga cgctcgccgg
    13321 gcgcgccgtt gtcgcggccg ccggcgcgca gcgctgcggc atgaaacgcc cgcacttgcg
    13381 cgggactctc ggcttggaac gcgaggtgca cccgcgacac cgggccgtcg gccttgtcga
    13441 tccagagctc gtccgagttg aaaaaatcgt cgccttcgcg gattccctgc gcgcggccga
    13501 gcgactcgag caccgcacgg tagaaccgct ttgaaaccgt aagatccgcc acgcgcaaat
    13561 gcacgtggtc gacgagacga ccacgatgaa aatgcattta gcttttcatg aacttggcga
    13621 tttcgggcgt tggcactgag cgctcgacgt agccgaacgt gcccttctct ttcacttcgc
    13681 gcgccgcttc catgaagccg ctcatggccg cgcgccagag cgatgtggcc aggctcacgc
    13741 gcttcacgcc cgccgcctcg agctcggcga cgctgaagga cttgcccttg atgccggcca
    13801 taaagttgaa gggcttcgag agcgaggcgc acacctttcg caccgattgc agatcgggca
    13861 gcccgggcgc catgagcacg tcggcgcccg ccttttcgaa cgcctgcagc cgccggatgg
    13921 tgtcgtcgag atccggccgg ctgcgcacga agttctccgt gcgcgcggtc agcacgagct
    13981 tcttgacccg tgctgcggcg gcgacccgct ccaccgcgtg ctcgaagtcg tacacgcgat
    14041 ccttcgtcgc atcctcgatt gagcagcccg cgaggccaat ctccgcggcg aggcgtactg
    14101 tttcggcggc ttcctgcgga tcgtcggcga agcagcgctc gaagtccgcg gagaccggaa
    14161 gctcggtcgc ctcgacaatc gcgcgagcgt gctccaacgc ctcctcacgc gtgaccatgc
    14221 catcgcgctt cccgagcgtg ccggcatgcg cgccgctcga tgtggcgagc gcctggaagc
    14281 caagcccggc aagaatgcgt gccgaccccg cgtcccaggc attggcgata acgaatgcgc
    14341 tgccgtgcat agcgcgaaag cgctgcacct tttctttctg gttcatctga tcacctcgtc
    14401 ggccctagcg agcaccgatt gtggaatctt cagatcaagc gcgttcgcgg tcttgaggtt
    14461 gaccgcgaga tacagccggt cgagctgctc gactggcaga tcgcccggct ttgcgccacg
    14521 aagaatgcgc tccacctggc cggcggcgag tccgccgagc gcatgatagc tcgcgccata
    14581 gctcgcgagg ccgcccttca tcacgaggtc gcggtcgttg aatatcgtcg ccaccttttt
    14641 cggattcagg atgtcgatga cgagctgcgc ctggctgatc accatcgagt cggccgagta
    14701 gaacgcgtcc gcatcccctg ggcgcagcgc ctcgaggctg gcacgcaatt cgtccaccga
    14761 ggcgatgtag cgctcggcaa cttcaacgtt cagcctgcgg gcggcatcgc gggtggtctt
    14821 gatgacctcg gtgttcgcgg ggttgtccgg ccggtagaag gtaacgacgc ggcgcaaacc
    14881 gggaatcatc tccttgagga gctcgaggcg ctttgcggca aggttcgtaa aacggctatg
    14941 gacgccggtc aagcggcccc cgggccggtg gaaactgtca acgaagccca tcgctactgg
    15001 atcgttgccg gcatagaaaa cgatcggcac tgttttcgtg ccgcgctttg cggccgcggt
    15061 gaccgacgtt gtcactgtga aaatgacgtc cacttgttcg cgctcgagcg cactcgctgt
    15121 cgactccacg gacttgagat cccctctggc ttcgcgctgg tgaaaaacga aatgcttgcc
    15181 ttcttcgagt ccgagctggg cgaggcgctc gcgcaggccc tcgagggccg cttgatacac
    15241 gcctccgtag agcagtacgc ctatgcgata gaggcgcggc tgctgcgcca ctcctgacag
    15301 cggcgcagca aagaacagca ggccgctcgc gagaacggcc cgcctggtca tcaccgtgct
    15361 agcggatcgg cggcgcgtac atgagcccgc cctgattcca caggcggttc agcccgcgct
    15421 cgaagccgag cggcttcagg ttgttgtcga agctctcgcc gtagttaccc acctgcttga
    15481 tgatgttgaa cgaccacttg ttgtcgatgc cgagcatctt gccgatgtcg ccgctcgcgc
    15541 cgacgaagcg ctggatggtc gggttggtgc tcgaggactc cttgtc
//
LOCUS       DASTPD010000331        18901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1409636, whole genome shotgun sequence.
ACCESSION   DASTPD010000331 DASTPD010000000
VERSION     DASTPD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409636"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFR57_11095"
     CDS             complement(<1..607)
                     /locus_tag="VFR57_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0259948.1"
                     /translation="MALASQTAAAFDLQGHRGARGHAPENTLAGFERALAIGVDTLEL
                     DVGVTRDGVVVIHHDRRLNPDVARGVDGKWVSAPAPTIYSLSYAELARYDVGRIRPGS
                     DYARRFPHQQPIDGTRIPRLSDLWPIAPRVRFNIETKLVPEAPEETLPPEPFARAVIA
                     EVRRAGVAPRTTIQSFDFRTLAVVQREAPEIRTAYLTSGQRG"
     gene            complement(625..999)
                     /locus_tag="VFR57_11100"
     CDS             complement(625..999)
                     /locus_tag="VFR57_11100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0259949.1"
                     /translation="MKDWDVGLVRHLGIRFVETGKDRVVGELEVRPELLTTTGALHGG
                     TLMAFADTIGAAGTVANLRPGQSTATLESKTNFFAAAFEGVVRAESTPLHRGKRTHVW
                     QTRITDANGKLLSLTIQTQVVL"
     gene            1060..1665
                     /locus_tag="VFR57_11105"
     CDS             1060..1665
                     /locus_tag="VFR57_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEU0259950.1"
                     /translation="MAQASVVACDTCGLVQALEPLAPGTVAECIRCGSFMGGRASARS
                     LEITAALSLAALILYVPANVYPIMKMYLYGGYSESTVWDGIVLLAENNQWFVAVVVFM
                     ASMVIPLVKLAGLFALVVTARTGWGQRLRERTQLYKFIDAVGPWAMLDVFLLAVLVAL
                     VKLSTWAKILPGPGLFAFTAVVVLTMLASAAFDPKLIWQRP"
     gene            1662..2528
                     /locus_tag="VFR57_11110"
     CDS             1662..2528
                     /locus_tag="VFR57_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0259951.1"
                     /translation="MTGEELPRARIRRRRLFRLVWVIPLVALAVAAYLVWQHMRSVGP
                     EILIRFNDASGLRVGQTPINYRGVQIGEVIGIELSEDRRRAVVKARLHKSAAPIATEG
                     AVFWIVRPQIGLSQVSGLNTVLSGPEIQVRPGNGEERLAEFNGLDNAPVGIDMPGLRL
                     VLRAERPKGIRLQTPVNYRGVEVGVVEKIELAPNAASADIHIVVRSRYAHLVRQGSAF
                     WNTSGISATGGILKGIELEIESLRTLYTGAIEFATPSDKAARVKSGTVFFLHDKPKDE
                     WLNWSPKFTADK"
     gene            2539..3228
                     /locus_tag="VFR57_11115"
     CDS             2539..3228
                     /locus_tag="VFR57_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0259952.1"
                     /translation="MATFVLVHGAWHGGWCWRFVRPLLGGHEVYAPSLTGLGERKHLA
                     HAGIDLETHIADVVSLLEMEDLRNVVLVGHSYGGMVITGAADRAPERIARLVYLDAFV
                     PENGKCVLDYVVPERAARMREEGAKVGDITPPPLSLWGITTQKHIDFIKPREVRHPYR
                     TMAQPIRLTGRTPPIPKTFLYCSSPATGSFDQFAAKYRNDRGWKFHELKTGHDAMILM
                     PGAVAEILLEK"
     gene            complement(3282..3569)
                     /locus_tag="VFR57_11120"
     CDS             complement(3282..3569)
                     /locus_tag="VFR57_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259953.1"
                     /translation="MKQGFYVLVLAAGLAGCGATPVEHKNYHDSDPSVGLFRDSLTWE
                     WRKKDTVNPPAQMTAAEQEEFKKWQDSASATERKEFEDWRAWQEWKRKNPK"
     gene            complement(3575..4711)
                     /locus_tag="VFR57_11125"
     CDS             complement(3575..4711)
                     /locus_tag="VFR57_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU0259954.1"
                     /translation="MKLQKTHLAAAVGVALSAYGTFALAQTPPPMSVQLYGQVSRALM
                     YADDGTQKKWFHVDNEASGTRLGATGTGTVMPGLRAGFRIEFDFQSNESQRVNFGPTS
                     PISNFPAHDEDVGGFAERHADAWIEGAWGRLNIGQGDGAANGATESDLSGTGMANGIG
                     VADLGGGFAHRTAAGALTAATVGGTINQQDFESRYDRLMYVTPNFAGFRGQVSWGQNA
                     NTDIKEASLWYGGKLGGLGEIAAAVGWSEQGAAAPGAAKDETIGGSISWLHGSGLNVT
                     LSYSNRDNPVAAGAAGRDSTFTYAKVGWKFGTHAIAVDYAMGDDFLAVGDEAKMYGIG
                     YVWNPIRWLELYAAYKIHELERSAANGGSLEDVTIGTIGTRVRF"
     gene            complement(4883..5497)
                     /locus_tag="VFR57_11130"
     CDS             complement(4883..5497)
                     /locus_tag="VFR57_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEU0259955.1"
                     /translation="MIDLYAAGTSNGMRARIGLEECGLKYTLHPIALEKGENKTPQFV
                     ALNPNAQIPVIVDHDGPGGKPVTVSQSSAILVYAAEKSGKFMPKDAAARPAFWQALMS
                     ASTDMTPTLGAVFQLARSKEPHAASIDLFKGRWKQYLKVWDDRLAKQKYAAGNEVTIA
                     DFSLYAGYARAKATVPDMCEGYSNVERWAKEMAARPAIQRALKF"
     gene            5580..5729
                     /locus_tag="VFR57_11135"
     CDS             5580..5729
                     /locus_tag="VFR57_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259956.1"
                     /translation="MKRAKSILFGATMFAIAAAANASAMYSVDENRDGRADREIVLEQ
                     SDSLA"
     gene            5798..6763
                     /locus_tag="VFR57_11140"
     CDS             5798..6763
                     /locus_tag="VFR57_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259957.1"
                     /translation="MAKKRAPAKRRPRKQKPGTRGLAPAETRLESLSGDAQAVAERVE
                     SVGGHLLGSYHDPLGKQPVLVAILPIDSVEPTPFQRDLSQVHHRKLAAVLDRTGMFLD
                     PIIAVTAPDKGFWTPNGMHRLMAMRRLGARAITALVIPKREIAWQILALNTEKAHNLR
                     EKALEAIRIYRGLLDEDDSRAESGFSFYLEEASLVTLGICYEKIGKFAGGAYNPIVRR
                     LETFSEEPIGKAIRTHEKRAQMLMDLDERVSEVVKKLKDKGFVSPYLRSFVVARINPL
                     RWIQGDPPPLDEVLRTMRERAAKFNVEKIKQSDLANIGGAAEDQD"
     gene            6883..7182
                     /locus_tag="VFR57_11145"
     CDS             6883..7182
                     /locus_tag="VFR57_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259958.1"
                     /translation="MRLAKSLLCGFSAVALSAGAAFAGQDSSFQGHASTEMSPPELLS
                     DENFGSSTSPSESASVMELERADELAGAMEGEVIYVYPVEVTEYYIIVPESQDLG"
     gene            complement(7229..7564)
                     /locus_tag="VFR57_11150"
     CDS             complement(7229..7564)
                     /locus_tag="VFR57_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259959.1"
                     /translation="MSIPTVERRRFKVGKYDIIANPIPNSAHMLRYTVFVGGKRIGAT
                     VSVPTESDCRFLEKPPVVPPLKIFSSYRPGRPKKGSRPQAPNESGHTSRESLPLDVSL
                     GGIRASTER"
     gene            complement(7561..7764)
                     /locus_tag="VFR57_11155"
     CDS             complement(7561..7764)
                     /locus_tag="VFR57_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259960.1"
                     /translation="MRLVKPGRSAVWTKERIDALTSAEVRQLRDNAERLSETDIVALC
                     DEILKARPRGASAKTVKAKRAAA"
     gene            complement(7767..8006)
                     /locus_tag="VFR57_11160"
     CDS             complement(7767..8006)
                     /locus_tag="VFR57_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259961.1"
                     /translation="MDKRKTWREQEQQLIEKWNSAARRYREIMARIADEQPFAPAGAP
                     SDDIMNQAAAVRAEIDALRKTVARMKAEFNSGKRY"
     gene            complement(8159..8686)
                     /locus_tag="VFR57_11165"
     CDS             complement(8159..8686)
                     /locus_tag="VFR57_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259962.1"
                     /translation="MYIVAALVKDAHQARGLIRALADAAFEREEIDMSGGPVSGLMAC
                     GVPEGEAHVLAEGIRRGGAVVAVRADDEMEAEQAALLMNRHGAVDVEACSTGWRSQGW
                     SGRITAPEDRIRIERYAYVFGDYPEGAGRIYRDPRTVASPSIDTPGAEPGGPYHGPER
                     RRMDKPYVGINRRAV"
     gene            complement(8730..9656)
                     /locus_tag="VFR57_11170"
     CDS             complement(8730..9656)
                     /locus_tag="VFR57_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259963.1"
                     /translation="MRKTRLSVAAALVLAAAGTAHAQQFEFASQGQAREILGRQDAYV
                     HSTAPRERSVILKTEASVTPERFARAMAETALEWSEEERRGFSEVLPRLQRFLAPMRW
                     KAPSTILLVKVSDRLMDGFPHTRANAIVLPEGMLRDALARPVLMDYLMAHEAFHVLTR
                     ADSQLREELYRAIGFRACETTELPAVLAEQRITNPDAPEKRYAIRLTRGEVMPFVHFV
                     PEPIDVTKGFSVHARTSWLPVDRHDGNCRARDERLTTDSLEGLYEQVGRNTGYLIHPE
                     EILADNFALLFRAPAKIASPEVPTRIERILRR"
     gene            complement(9637..11451)
                     /locus_tag="VFR57_11175"
     CDS             complement(9637..11451)
                     /locus_tag="VFR57_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0259964.1"
                     /translation="MGGPPAGLAAFYWHFVRQTKGWYAAMFAASLAVAALDTVIPLFI
                     GRLVSLMEAGDRMAALREQWPMLVGMIALVLVVRPLVLLADIAIRHMVLIPGATSLIR
                     WQSHWHVIRQSWPFFQNDFAGRIANRVMQTAHALRESVMASIRAVWYIGAYGVITYAL
                     MAMADWRLGVPTLVWFAGYLVFLWHFVPRMRDLARESSEARSLVMARVVDSYTNVLTV
                     KLFARLADEDAYVRESIDEHRAAMRRHMRLITRFHFGLTAMNAALLVATATIGIWLWA
                     SGAVGAGVVAAALPLTWQLANVAGWVSWEVTAIFENVGVVQEGMQSIAVPHSSVDKPG
                     AQPLEVSRGEIRFEHVRFGYGRPEAVLEGFELGIKPGERVGLVGRSGAGKSTIVNLLL
                     RFFDLEKGRILIDSQDISQVTQESLRAAIGVVTQDTSLLHRSIAANIRYGRPRASDEE
                     VIAAAKKAQAHEFILGLRDWRGNAGYAAHVGERGVKLSGGQRQRIALARVILKDAPIL
                     VLDEATSALDSEVELAIQEQLLDLMEGKTVIAIAHRLSTIARMDRLVVVERGKIVESG
                     THDELLRRGGHYEKLWRHQSGGFLADDVFSSDIAEDPA"
     gene            11595..12476
                     /locus_tag="VFR57_11180"
     CDS             11595..12476
                     /locus_tag="VFR57_11180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0259965.1"
                     /translation="MKAITQPEIDQQVFNLYDEYCHGRIDRREFVRRAGALGAGALAM
                     AYGLMPNYARAQTISFTDSRIKASYVTYPSPGGNGDKIRGYLVQPAGNGPWPAILVIH
                     ENRGLNPYIEDVARRAAVDGFLALAPDGLTPLGGYPGNDDDGRALQAKLDQAKLRQDM
                     LNGARWLKNHELSTKRLGATGFCWGGGTTNFLAAAMGSDLQAGVPYYGAAADTASVPK
                     IQAPLLAHLAEQDPRINEMYPTYQEELKKAGVRHEIHMYPGTQHGFHNNSTPRYADAA
                     AKLSWERTIAFFKKNLA"
     gene            complement(12480..13442)
                     /locus_tag="VFR57_11185"
     CDS             complement(12480..13442)
                     /locus_tag="VFR57_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259966.1"
                     /translation="MNAFRCLLILVAALAASAHAQPYPSKPIRLLVGFAPGGNTDTVA
                     RVVGQKLGERLGQQVVIENKAGAGGTIATGEVARAAPDGYTLTMGTTTTHAIAVAAYS
                     KLNYDPNADFEPLAMVAIAPYLLVVHSKTPARNVKEFVELVKSQPGKLNYGSAGQATT
                     THLVMASFAHRAGLDVVHVPFKGNAPATQAILGGQIEILFGGIPPLLPHVKAGTLRPL
                     AISSAKRSPSLPDVPSIAESGYPGFDISLWLGFFAPKGTPAAVTKRLESELLQIAQSS
                     DVKEIMDKQGLEAAAGTAADLAKLVKTEIEVYKTVFKAANIKME"
     gene            13441..14310
                     /locus_tag="VFR57_11190"
     CDS             13441..14310
                     /locus_tag="VFR57_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0259967.1"
                     /translation="MGSSWGLNISVMRIAFIGFGEVATAFSSALAARGARIAAYDVRP
                     DQREAAERLGVSFGTLSDALQDADYVFSTVTTSVARLAAEQCAPRLRPGQHYVDLNAT
                     DPDAKKEIQRVIEPSRAAFVEGALLGAVGVTGAKTEILLGGPAARRAEQDLAGTLGLN
                     ARFYSEEIGKASMFKMLRSVFSKGMEALLIEFLAAGERAGIRDDLWKEVTGLFATQPF
                     EKVAANWVRSHASAHERRYHEVKQVAAVLRALGIEPTITAATEAFFARSASLGLVPAA
                     SMDEVVRRFNERL"
     gene            14307..15068
                     /locus_tag="VFR57_11195"
     CDS             14307..15068
                     /locus_tag="VFR57_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0259968.1"
                     /translation="MKKNRMKEKLRRGEAVFGVSVMIPSPQIVEMIAAAGFDWVLLDC
                     EHGTLTLESVELMAMAAEASGITAIARPVSRAPEHILQVLDRGVMGVQVPHVNTGADA
                     RRVLEAVKYHPLGLRSLAAGTRAAVYDSHGTLADYVAAANNETLVAVQLEDREAIDNI
                     DELLKVDNIDVFFIGPSDLSQSMGHPGNPKAHAVAEAIDSSFRKMIAAGRTPGTPATA
                     DNLRGVLDKGVRYIYTHLPRLLSSSAKSYLGHARA"
     gene            15055..16017
                     /locus_tag="VFR57_11200"
     CDS             15055..16017
                     /locus_tag="VFR57_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259969.1"
                     /translation="MRALKRFLLVFSLLPTLVVAQGYPTKAVKIIVPAQPGGGLDLIG
                     RTVGAELGKMFDQQFVIENVGGGGGSIACQQTARAAPDGYTLMVGYVATHATSPAVRK
                     VPYDAIRDFTPIAMVGGTRNMLVVHPNVPVSNLKEFIDYARGKTGKVTFGAGGIGTLN
                     HLLIEQLKGAAGFDAVIAQYKGIGPAFADLFGGQLEALTPGVAAALPHIRNKRVKPIA
                     VTGLTRNALLPEVPTFEEAGFKGFDGVQWYGIVGPAKLPTAIVERLNAEIGKALASPA
                     LQQRLVAEAIEPMPMTPNEFGAFIKAELERYTVLARDRNIRLDE"
     gene            complement(16076..16822)
                     /locus_tag="VFR57_11205"
     CDS             complement(16076..16822)
                     /locus_tag="VFR57_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0259970.1"
                     /translation="MKIATWNVNGIRARQAQFCEWVAREHPDVVCLQELKAEPTQIPE
                     QCKVVDYDTFWHGMRAYSGVSLHIRKGAFETPPAFSHPAFDMESRIVQAQLGELLIAS
                     IYVPNGNKDYPAKIDFVRRLIAWVREQHDQGRELILCGDMNITHTEMDVHPRERKPQL
                     VGQRPEERALFTELLNQGLVDVGRKLDPHNAGLFTWWAPWRNMRQRNIGWRLDYILAS
                     AGLAVRAQSCMVQADFGTSDHAPVVMTTAD"
     gene            16849..18240
                     /locus_tag="VFR57_11210"
     CDS             16849..18240
                     /locus_tag="VFR57_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0259971.1"
                     /translation="MKRIADLPGPRALPILGNLLQIDKASLHLIAEKWSRQYGEVFRF
                     RIGCRQLVALANPETIAAVLRERPDGFHRTERLSATAREMGFNGVFSANGDAWRRQRP
                     MVMAGFDPAHIKSYFPMLVNVTERFSRRWQRAAAAGTAIDLQADLMRYTVDVTAGLAF
                     GSDINTLESEEDVIQAHLDKIFPALFKRLLAPVPYWRWFPDRKVERHVVALRRAVEGF
                     IAQARARMSNPPRNLIEAMIAARDTPGSGLTDDDVAGNVLTMLLAGEDTTANTLAWMI
                     YLLHRHPEALARAGEEVSRVRLDRYEDVSALPFVDACINETMRLKPVAPIIMAQPTRD
                     TEIAGVQVPKKTIVMCLMRAGAIDERRFADAKAFEPGRWLSGAQASSPKRVSMPFGAG
                     PRLCPGRYLAILEMKMVMAMLLSNFGIESVGTPDGGEAREHLAFTMSPVGLKLKLRSG
                     ATAKASTLLARSA"
     gene            complement(18188..>18901)
                     /locus_tag="VFR57_11215"
     CDS             complement(18188..>18901)
                     /locus_tag="VFR57_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0259972.1"
                     /translation="MNEETIAGVVGWGRTVGAVASMISVDLYEAGRIRRTMPKGGDKY
                     TIFRIGEPHGRITPRVEELVKWFSGIDSSKATTNLWGERWSKLVQNSMGNGVTAATGL
                     TSPACLANEKIRRFQIALAGEAIRVGQALGYTLEKVRALEPEKFARAAEGDKAALAEV
                     EAAMIPKEGANPRGDIQRPSMAQDILKGRRTEIDAMNGFIARKGKEVGVAAPSHEKLA
                     ALVTRIERGELKPSPSLLT"
BASE COUNT         3242 a         6173 c         6137 g         3349 t
ORIGIN      
        1 cgccgcgctg cccggacgtg aggtaagcgg tgcggatctc gggcgcttcg cgctgtacca
       61 ccgcgagcgt gcggaagtcg aacgactgga tcgtggtgcg cggcgccacg ccggcccttc
      121 gtacttccgc aatcaccgct cgcgcgaaag gctccggtgg cagcgtctcc tccggtgcct
      181 cgggaacgag cttcgtttcg atgttgaagc ggacccgcgg agcgatcggc cacagatccg
      241 aaagccgagg gatgcgcgtg ccgtcgattg gctgctggtg tgggaagcgt cgcgcgtagt
      301 ccgagcccgg ccgaatacgg ccgacgtcgt aacgcgcaag ctcggcgtag gagagtgaat
      361 agattgtggg cgcgggcgcg ctgacccact tcccgtccac gccgcgcgcc acatccgggt
      421 tgagccgccg gtcgtggtga atcaccacca cgccgtcgcg cgtcacgccg acgtcgagct
      481 cgagcgtgtc gacaccgatc gcgagcgcgc gctcaaaccc cgcgagcgtg ttctccggcg
      541 catggccgcg cgcgccccgg tgaccctgaa gatcgaatgc cgccgccgtc tgcgaagcca
      601 gtgccataac gagcaggagc cggcttatag gaccacctgg gtctggatcg tgagcgaaag
      661 gagcttgccg ttcgcatccg ttatgcgcgt ctgccagacg tgcgtgcgct tgcctcggtg
      721 aagcggcgtt gattcggcgc gcaccacccc ctcgaacgcg gcggcgaaga agttggtctt
      781 cgattcgagc gtcgccgtac tttgcccggg ccgcaaattc gccaccgtgc ctgccgcacc
      841 gatggtgtcg gcgaacgcca taagcgtgcc gccgtggagc gcgccggtgg tcgtaagaag
      901 ctccggccgg acctcgagct ccccgacgac gcggtcctta cccgtttcga caaagcggat
      961 acccaggtgc cgaacgaggc ctacgtccca gtccttcatg gtccggaagg tacatgaggc
     1021 gcgccgcgct tgggcatgct gtttgcaaag cgatgccaga tggcccaagc gtcggtagtt
     1081 gcttgcgata cctgcggcct ggtccaagcc ctcgagcccc ttgcgccggg caccgtggcg
     1141 gagtgcatcc gctgcggctc gttcatgggc ggccgggcga gcgcgcgcag cctggagatc
     1201 acggccgcgt tgtccctcgc cgcgctcatt ctttacgtgc cagcgaacgt ctatcccatc
     1261 atgaagatgt acctctacgg cggctactcc gagagcacgg tgtgggacgg catcgtgctc
     1321 ctcgccgaga acaatcagtg gttcgtcgcg gtcgtcgtgt tcatggcgag catggtgatt
     1381 ccgctcgtca agctcgccgg gctgttcgcg ctcgtcgtca cggcgcgcac gggatggggt
     1441 cagcgcctgc gcgagcgcac ccagctctat aaattcatcg acgcagtcgg gccgtgggcg
     1501 atgctcgacg tcttcctcct cgccgtgctc gtggctctcg taaagctcag cacctgggcg
     1561 aagatcctgc ccggcccggg gctcttcgcg ttcaccgccg tggtggtgct caccatgctc
     1621 gcttcggcgg cgttcgatcc gaagctcatc tggcagcggc catgaccggc gaagagctac
     1681 cgcgcgctcg catccgccgt cggcgcctgt tccgcctggt gtgggtcatt cccctggtcg
     1741 cgctcgccgt ggccgcctac cttgtgtggc agcacatgcg ttcggtcgga cccgagattt
     1801 tgatccgctt caacgacgcg agcggcctgc gcgtggggca aacgcccatc aactatcgcg
     1861 gcgtgcagat cggcgaagtc atcggaatcg agctctcgga ggatcgacgg cgcgccgtgg
     1921 tcaaggcgcg cctgcataag tcggcggcgc cgatcgcgac cgagggtgcg gtgttctgga
     1981 tcgtgcggcc gcagatcggc ctcagccagg tgagtggcct caacaccgtg ctgagcggcc
     2041 cggagatcca agtgcggccc ggcaacggcg aagagcgcct cgccgagttc aacggcctcg
     2101 acaacgcgcc agtcggcatc gacatgcccg gcttgcgcct cgtgctgcgc gcggagcgcc
     2161 cgaagggcat ccgcctgcag acgccggtca actaccgcgg tgtcgaagtc ggcgtggtgg
     2221 agaaaatcga gcttgcgccc aatgccgcgt ccgccgacat ccacatcgtg gtgcgcagcc
     2281 gttacgccca ccttgtgcgt cagggctccg ccttctggaa tacgagcggc atttcggcga
     2341 cgggcggcat cctgaaaggc atcgaactcg agatcgagtc gttgcgcacg ctctacaccg
     2401 gcgccatcga gttcgccaca ccgagcgaca aggcagcgcg cgtcaaatcg ggcacggtct
     2461 tcttccttca cgacaagccg aaggacgagt ggctgaactg gtcaccgaaa ttcacggccg
     2521 acaaatagaa tccgccccat ggcgacattc gtgctggtgc acggcgcctg gcacggcggc
     2581 tggtgctggc gcttcgtacg cccgctcctc ggcgggcacg aggtgtatgc gccttcgctc
     2641 accgggctcg gcgagcgcaa gcacctcgcg cacgctggta tcgacctcga aacgcacatc
     2701 gccgacgtcg tgtcgctcct ggaaatggaa gacctgcgca acgtggttct cgttggccac
     2761 agttacggcg gcatggtgat caccggcgcc gccgatcgcg cgcccgaacg aatcgcgcgc
     2821 ctcgtctatc tcgacgcgtt cgttcccgag aacggcaagt gcgtcctcga ctacgtcgtg
     2881 ccggaacgcg ccgcccgcat gcgggaggag ggcgcgaagg tcggtgacat cacgccgccg
     2941 cccctttcgc tttggggcat tacgacgcag aagcacatcg acttcatcaa gccgcgcgaa
     3001 gtgcgccatc cctatcgcac catggcgcag cccatccggt tgacgggtcg gacgccgccc
     3061 atccccaaga ccttcctcta ctgctcatcc ccggccaccg gctccttcga ccagttcgcc
     3121 gcgaagtacc ggaacgatcg ggggtggaag tttcatgagc tgaaaaccgg ccacgacgcg
     3181 atgattctta tgccgggcgc ggttgcggag atcctgctcg agaaataaaa aggggtcgga
     3241 gatttttcgc tccgaccccg gtactgctcc ctgagcgttt tctacttcgg gttcttgcgc
     3301 ttccactcct gccaggcgcg ccagtcttcg aactccttgc gctccgtggc acttgcggag
     3361 tcctgccact tcttgaactc ttcctgctcg gcggcagtca tctgagccgg cggattgacg
     3421 gtgtctttct tccgccactc ccaggtcaga ctgtccctga agagccccac gctgggatcg
     3481 ctgtcgtggt aattcttgtg ctctaccgga gtggccccgc aaccggcgag gccggccgcg
     3541 aggacgagaa cgtagaaccc ttgcttcatg tttcctagaa cctcactcgt gtgccgatgg
     3601 tgccaatcgt gacgtcctca agggaacccc cgttcgcggc ggaacgctcc agctcgtgga
     3661 tcttgtaggc ggcataaagc tccagccacc ggatcggatt ccagacgtag ccgatgccgt
     3721 acatcttggc ctcgtcgccg acggcgagga agtcgtcgcc catcgcgtag tcgaccgcga
     3781 tggcgtgcgt gccaaacttc cagcccacct tggcataagt gaaggtcgag tctcggcccg
     3841 cggcaccggc ggcgacgggg ttgtcgcggt tggagtagct cagagtcacg ttcaagccgg
     3901 agccatgcaa ccaggagatc gagccgccga tggtttcgtc cttggcggcg cccggcgcag
     3961 ctgcgccttg ctccgaccag ccaacggccg ccgcaatctc acccagaccg ccgagcttgc
     4021 cgccgtacca gagcgacgcc tctttgatgt cggtgttcgc gttctggccc cagctcacct
     4081 ggccgcgaaa gccggcgaag ttgggcgtca cgtacatgag gcggtcgtag cggctctcga
     4141 agtcctgctg gttgatcgtc ccgccgacgg tagcggcggt cagcgcgccg gcagcggtcc
     4201 ggtgggcgaa accgccgccc aagtccgcca cgccgatgcc gttcgccatg cccgtgccgg
     4261 acaggtcgct ctccgtcgcg ccgttggcag cgccgtcgcc ctggccgatg ttgagacggc
     4321 cccaagcacc ctcgatccac gcgtctgcgt ggcgctcggc gaagccgccg acgtcttcat
     4381 cgtgcgccgg gaagttggag atcggactcg tcggaccgaa gttcacgcgc tgcgactcgt
     4441 tcgactggaa gtcgaactcg atgcggaacc cggcgcgcag gcccggcatt accgttccgg
     4501 tgccggttgc cccgaggcgc gtgccgctcg cctcgttgtc aacgtggaac cacttcttct
     4561 gcgtgccgtc atcggcatac atcaacgcgc ggctgacctg accgtagagt tgcaccgaca
     4621 tgggcggcgg cgtctgcgca agtgcgaagg tgccgtatgc agacaacgcc actccgactg
     4681 cggcagcgag gtgcgttttt tgaagcttca ttgaaccccc tgttgtgcgt gattcgttgg
     4741 ttaacccgga caccaactcc ggggcgcgac ggattatagg gaagggccgc acaaaaccgc
     4801 acaaaccact ggaactctgt tgttttttcg catcatgcga ttgcgcgcgc gcgccgttgg
     4861 tgcaccgcaa gagcgcgcca aatcaaaact taagcgcccg ctggatcgcc ggccgggccg
     4921 ccatttcctt cgcccagcgc tcgacattcg aatagccctc gcacatgtct gggactgttg
     4981 ccttcgctcg agcgtagccg gcgtaaagcg aaaaatcggc gatcgtgact tcgttccctg
     5041 ccgcgtactt ctgcttggcg agtcggtcgt cccagacctt gaggtattgc ttccagcggc
     5101 ccttgaagag gtcgatgctc gcagcgtgcg gctccttgga acgcgcgagc tgaaacaccg
     5161 cgcctagcgt gggcgtcatg tccgtggatg cgctcatcag cgcctgccag aacgccgggc
     5221 gcgccgccgc atccttcggc atgaacttgc ccgacttctc ggcggcgtag acgaggatcg
     5281 cggaggattg ggaaacggtc accggttttc cgccgggccc gtcgtggtcg acgatcaccg
     5341 gtatctgcgc attgggattg agcgcgacga attgcggcgt cttgttctcg cctttctcaa
     5401 gcgcgatggg gtgcagcgtg tacttcagcc cgcattcctc gagcccgatg cgcgcgcgca
     5461 ttccgttcga agtgcccgcc gcgtatagat cgatcattcg aagagacctc cttcgcgccg
     5521 atggtatgcc ggcatgattc ctgcggcaat gcgcgggtga cgttgcgaaa ggagaaaccg
     5581 tgaaacgagc gaagtccatc ctttttggcg cgaccatgtt tgcgatagcg gcggcggcca
     5641 atgcctcggc catgtactcc gtcgacgaga atcgcgacgg ccgtgcggat cgcgagatcg
     5701 tgctggaaca atcggactct ctggcatagc cgcatccgaa gcgtcatgga acgggccgca
     5761 aggcccgttt cttttttcgc ctgcgataat tcgcgccatg gcgaagaagc gcgccccggc
     5821 caagcgccgg ccacgcaagc agaagcccgg aacgcgcggc ctcgcgccgg cggagacgcg
     5881 gctcgagagc ctgagtggcg acgctcaggc ggtcgccgaa cgggtcgaga gcgtcggcgg
     5941 tcatctgctc ggcagctacc acgacccgct cggcaagcag ccggtgctcg tcgcaatcct
     6001 gcctatcgac agcgtcgagc cgacgccatt ccagcgcgat ttatcgcagg tgcatcaccg
     6061 taagctcgcc gctgtgctcg atcgcaccgg catgtttctc gatccgatca tcgccgtgac
     6121 cgcaccggac aagggatttt ggacgcccaa cggcatgcac cggctgatgg cgatgcggcg
     6181 cctgggcgct cgcgcgatca cggcgctcgt catcccgaag cgcgagatcg catggcagat
     6241 cctcgcgctg aacaccgaaa aggcgcacaa cctgcgcgag aaggcgcttg aggcgatccg
     6301 catctatcgc ggccttctgg acgaggacga ttcacgcgca gagtccggct tctcgttcta
     6361 tttggaagaa gcgtcgctcg tcacgctggg catctgctac gagaagatcg gcaagttcgc
     6421 cggcggcgcg tacaacccca tcgtacgccg cctcgaaacc ttctcggagg agccgatcgg
     6481 caaggcgatt cgcacgcacg aaaagcgcgc acagatgctc atggacctcg atgagcgcgt
     6541 cagcgaagtc gtgaagaagc tgaaggacaa gggcttcgtg agtccctatc tgcgtagttt
     6601 cgtcgtggcg cgcatcaatc cgctgcgctg gatccagggc gatccgccgc cgctcgacga
     6661 agtgctcagg acaatgcgag agcgcgccgc caagttcaac gtcgagaaga tcaagcagtc
     6721 ggacctcgcg aacatcggcg gcgcggcgga agaccaggac tagctggaag tcttgcagcg
     6781 ataggccccg cgggttacac gttttgcggg gacttccctt acgaggaaag cccccggcac
     6841 gcaacctgcg gcattcgggt atcccatgtc aaaggagaga ccatgagatt ggccaagtca
     6901 ttactttgcg gtttttcggc ggtggcgctt tccgcgggcg ccgctttcgc ggggcaggac
     6961 tccagttttc aagggcacgc gtccaccgag atgagcccgc cggaacttct tagcgatgag
     7021 aacttcggct cgtcgacgtc gccgagcgag agcgcctctg tgatggagct cgagcgcgcg
     7081 gacgaacttg ccggcgcgat ggagggtgaa gtgatctacg tctacccggt ggaggtcacc
     7141 gagtactaca tcatcgtccc ggaatcgcag gacctgggat agcgaagacg aaattggccc
     7201 ccgctttcgc gggggcgact acttagttct agcgctcggt cgaggcgcgg atgccgccga
     7261 gcgagacatc gagcggcagc gactcgcggg aggtatgtcc gctctcgttc ggggcctgcg
     7321 gacggctgcc tttcttcgga cggccgggac gatagctcga gaagatcttg agcggcggaa
     7381 cgaccggcgg cttctccagg aagcggcagt ccgattcggt cggcacgctc accgttgcgc
     7441 cgatgcgctt gccgccgacg aacacggtgt agcgcagcat gtgcgcggag ttcggaatgg
     7501 gattggcaat gatgtcgtac ttgccgactt tgaaacgacg acgctccacg gtggggatac
     7561 tcatgctgcc gcccgcttgg ctttgactgt cttggcggaa gcaccccgcg gccgcgcctt
     7621 aaggatctca tcgcacagcg cgacgatgtc ggtttcggag agccgctccg cgttgtcgcg
     7681 aagctgccgg acctcggcgc tggtgagcgc atcgatcctt tccttggtcc agaccgccga
     7741 gcggccgggt ttaaccagtc tcatcatcag tatcgctttc cagagttgaa ttccgccttc
     7801 atccgcgcca cggtcttgcg cagcgcatcg atctccgcgc gtacggctgc ggcctgattc
     7861 atgatgtcgt cgctcggcgc gccggcgggg gcaaaaggct gttcgtcggc gatcctggcc
     7921 atgatctccc ggtagcgccg ggccgctgag ttccacttct cgatcagctg ttgctcttgc
     7981 tcgcgccagg tcttgcgctt gtccacgaat atccttttgc gcccggtctt tcagcgggcg
     8041 aaaactgccg aggaacggag gaagctgcgg ataagaaccc gcagattacc acattcccga
     8101 gctaaattcg ggacttagtc gaaacaacga caaccgccta aggaaacctt ccgctcgtct
     8161 acacggcgcg ccggttgatc ccaacgtagg gcttgtccat gcgccggcgc tcggggccgt
     8221 gatagggccc gccaggctct gcgccggggg tatcgatcga gggcgacgct acggtgcgcg
     8281 gatcgcgata gatccggccg gcgccctcgg gatagtcgcc gaatacatag gcatagcgct
     8341 cgatacggat gcggtcttcc ggggcggtaa tccggccgct ccagccctgg ctgcgccagc
     8401 ccgtcgagca tgcctcgacg tcgacggcgc cgtgccggtt catgagcaac gcggcctgct
     8461 cggcctccat ctcgtcgtcg gctcgcacgg ccacgacagc gccgccgcgc cggatccctt
     8521 ccgcaaggac gtgcgcctcg ccttccggga ctccgcacgc cataaggccg ctcaccggcc
     8581 caccgctcat gtcgatctct tcacgctcga atgccgcgtc cgcgagggcg cggatcaggc
     8641 cgcgcgcctg gtgcgcgtcc ttcaccaatg ccgcaacgat gtacataaaa agctcctttc
     8701 agaccccgcg gagcaagggg catgccgcgc tagcgacgca ggatccgctc gatacgggtc
     8761 ggtacctcgg gcgaagcgat cttggcgggg gcacggaaga gcagcgcgaa gttatcggcg
     8821 aggatctcct ccggatggat caggtaaccg gtattccggc ccacctgttc gtatagtcct
     8881 tccaggctgt ccgtggtgag gcgttcgtcg cgcgcacggc aattgccatc gtgccggtcg
     8941 acgggcagcc acgacgtccg tgcatgaacg ctgaatcctt tcgtcacatc gatgggctcc
     9001 ggaacaaagt gaacgaaggg catgacctcg ccgcgggtca ggcgaatcgc gtaccgcttt
     9061 tcgggcgcgt ccgggttcgt gatgcgctgc tcggcgagca cggccggaag ctcggtcgtc
     9121 tcgcacgcgc gaaaacctat ggcgcggtac agctcctcgc gcagttggga atctgccctc
     9181 gtcagcacat ggaacgcctc gtgcgccatc aggtagtcca tgagcaccgg cctggcgagc
     9241 gcgtcgcgca gcatgccttc cggaagcacg atggcgttcg cccgcgtatg cgggaagccg
     9301 tccatcagcc ggtccgaaac cttgacgagg aggatggtgg acggcgcctt ccagcgcatc
     9361 ggcgcaagaa aacgttgcag ccgcggcagc acctcggaga agccgcggcg ctcctcctcg
     9421 ctccattcga gcgccgtctc ggccatggct cgcgcgaaac gctcgggcgt tacggatgct
     9481 tcggttttca ggataactga gcgctcgcgc ggcgcggtcg agtgaacgta cgcgtcctgc
     9541 cgaccaagga tctcgcgtgc ctgtccttgc gaagcgaact cgaactgctg tgcgtgcgcg
     9601 gtacccgctg cggcgagaac gagcgccgcg gcgacgctaa gccgggtctt ccgcaatatc
     9661 ggaggaaaac acgtcatcgg cgaggaagcc gcccgactgg tggcgccaga gcttctcgta
     9721 atgcccgccg cggcgcagga gctcgtcgtg cgtgccgctc tcgacgatct ttccgcgctc
     9781 caccaccacg agccggtcca tgcgcgcaat ggtggaaagc cgatgcgcaa tggcgatcac
     9841 cgtctttcct tccatgaggt cgagcagctg ctcctggatg gcgagctcga cttcgctgtc
     9901 gagcgcggac gtggcctcgt cgaggacgag gatcggcgcg tccttgagga tcactcttgc
     9961 gagcgcgatg cgctgccgct gcccacccga cagcttcacg ccgcgctcgc cgacgtgcgc
    10021 cgcgtagccg gcgttgccgc gccagtcgcg taagccgagg atgaactcat gcgcctgcgc
    10081 tttctttgcg gccgcgatga cctcttcgtc gctcgcgcgc ggccggccat agcggatgtt
    10141 cgccgcgatg gagcggtgga ggagggacgt atcctgcgtg accacgccga tcgccgcacg
    10201 caggctctcc tgcgtgacct gagagatgtc ctggctgtcg atgagaatgc ggcccttctc
    10261 gagatcgaag aagcgcagca gcaggttgac gatcgtcgat ttgcccgcgc ccgaacggcc
    10321 gaccaaaccg acgcgctcgc cgggcttgat cccgagctcg aagccttcga gcaccgcctc
    10381 gggccgcccg tagccgaaac gcacgtgctc gaaccgtatc tcgccgcgcg agacctcaag
    10441 cggctgcgcg ccgggcttgt cgacgctgga atgcggcacg gcgatggact gcatgccttc
    10501 ctgcaccacg ccgacatttt caaagatggc ggtgacttcc cagctcaccc agccggccac
    10561 gttcgcgagc tgccaggtga gcggcagcgc agcggcgacg actccggcgc ccacggcgcc
    10621 gctcgcccac agccagatgc cgatcgtcgc ggtggcaacg aggagcgcgg cgttcatcgc
    10681 ggtgaggccg aaatggaagc gcgtgatgag ccgcatatgc cggcgcatgg cagcgcggtg
    10741 ctcgtcgatc gactcgcgca catacgcatc ttcgtcggca agccgcgcga agagcttcac
    10801 cgtgagcacg ttggtgtagc tgtcgaccac gcgtgccatg acgagcgagc gcgcttctga
    10861 gctttcgcgc gcgagatcgc gcatgcgcgg cacgaagtgc cagaggaaga cgagatagcc
    10921 ggcgaaccag acgagcgtcg gcacccccaa acgccagtcg gccatcgcca tcagcgcgta
    10981 cgtgatgacg ccgtatgcgc cgatgtacca gacggcgcga atgctggcca tgacgctctc
    11041 gcgcagcgcg tgtgcggtct gcatgacgcg gttggcgatg cggccggcaa aatcgttctg
    11101 gaagaaaggc cagctttggc ggatcacatg ccagtggctc tgccagcgga tgagcgaggt
    11161 cgcgccgggg atgagaacca tgtgccggat cgcaatatcg gccagcagca cgagcggccg
    11221 cacgacgagg acgagggcga tcatgccgac gagcatcggc cactgctcgc gcagcgccgc
    11281 catgcggtcg cctgcctcca tgagcgatac caggcgcccg atgaagaggg gtatcacggt
    11341 atcgagcgcc gcgacagcga ggcttgcggc gaacatcgcc gcataccacc ctttcgtctg
    11401 gcgcacgaag tgccaataga acgcggcgag acccgccgga ggaccaccca tgacgctatt
    11461 tcggctttcg actttctaaa gcagaacaat agcatgcgcg ggcgaattca tgttggtcct
    11521 gaaccggaat tcgacctgct tggccgcgtt taccggagac gggctagcat cgtccagctc
    11581 agcacggaga accaatgaaa gccatcactc agccggaaat cgaccagcag gttttcaatc
    11641 tgtacgacga gtactgtcac ggccgcatcg accgccggga attcgtgagg cgcgccggcg
    11701 cgctcggggc cggcgcgctc gccatggcgt acggtctgat gccgaattac gcacgcgcgc
    11761 agaccatttc atttaccgac tcgcgcatca aggcgagcta tgtgacttac ccctctccgg
    11821 gtggcaatgg cgacaagatc cggggctact tagtacagcc ggccggcaac gggccgtggc
    11881 cggcgattct cgtgattcac gaaaaccgcg gcctcaatcc ttacatcgag gacgtcgcgc
    11941 gtcgcgccgc cgtggacggt ttccttgcgc tcgcgcccga cgggctaacg cccctcggcg
    12001 gttatccggg caacgacgac gacgggcgcg cgctgcaggc gaagctcgac caggcgaagc
    12061 tgcgccagga catgctgaac ggcgcgcgct ggctcaaaaa ccacgagctc tcgacgaaga
    12121 ggctcggcgc caccggcttc tgctggggcg gcgggaccac gaacttcctc gccgccgcga
    12181 tgggttcaga ccttcaggcc ggcgtgcctt attacggcgc cgccgcggac accgcctccg
    12241 ttccgaagat ccaggcgcct cttctcgccc acctcgcgga gcaggacccg cgtatcaacg
    12301 agatgtaccc gacctatcag gaagagctga agaaggcagg cgtgcgccac gagatccata
    12361 tgtatccggg cacgcagcat ggcttccaca acaactcgac gccgcgttac gccgacgcgg
    12421 cggctaagct ctcatgggag cgcacgatcg ccttcttcaa aaagaacctc gcgtgagcgc
    12481 tattccattt tgatgttggc tgccttaaac acggtcttgt aaacctcgat ttcggtcttc
    12541 accagcttgg cgaggtcggc cgccgtgccc gcggcggctt caagaccctg tttgtccatg
    12601 atttctttga cgtcggacga ctgggcgatc tgcaggagct cgctctcgag ccgcttcgtc
    12661 accgcggccg gggtgccctt cggcgcgaag aatccgagcc agagcgagat atcgaagccg
    12721 gggtagccgg actcggcgat tgacggtacg tcggggagag agggcgagcg cttggccgag
    12781 ctgatggcga gcggacgcag cgtgccggct ttgacgtgcg gaaggagcgg cggaataccg
    12841 ccgaacagaa tctcgatctg accgccgagg atcgcctggg tcgcaggggc gttgcccttg
    12901 aacgggacat gcaccacatc gaggccggcg cggtgcgcga agctcgccat cacgagatgc
    12961 gtcgtcgttg cctgccccgc cgagccgtaa ttcagtttgc cgggctgcga tttcacgagc
    13021 tcgacgaatt ccttgacgtt gcgcgcgggc gtcttggaat gcacgacgag cagataggga
    13081 gcgatggcga ccatcgcgag cggctcgaaa tcggcgttcg ggtcgtagtt gagcttcgaa
    13141 tacgcggcga cggcgatcgc gtgcgtcgtg gtggtgccca tggtgagggt gtacccgtcg
    13201 ggcgccgccc gcgccacttc gccggtggca atggtgccgc cggcgccggc tttgttttcg
    13261 atcacgacct gttggccgag gcgttcgccg agcttctgtc cgactacgcg cgcgaccgtg
    13321 tcggtgttgc ccccgggcgc gaagccgaca agcaggcgaa tcggcttgct cgggtaaggc
    13381 tgcgcatgcg cgctcgcagc gagagctgca acgagaatca acaggcatcg aaacgcgttc
    13441 atcggatcct cctgggggct taacattagt gtaatgcgca tcgcgttcat cggattcggc
    13501 gaggtcgcga cagccttttc cagcgcgctc gcggcacgcg gtgcgcgcat cgcggcctac
    13561 gacgtgcggc ccgaccagcg cgaggcggcg gagcgcctgg gcgtgtcgtt cggcacgctt
    13621 tccgacgcgt tacaggatgc ggactatgtt ttttccaccg tgacgacgag cgtcgcgcgc
    13681 ctcgccgcgg aacaatgcgc cccgcgtttg cggccgggac agcactacgt cgacctgaac
    13741 gccacggatc ccgacgcgaa aaaagagatc cagcgtgtca tcgagccgtc gcgtgccgcg
    13801 ttcgtcgagg gtgcgctcct gggcgccgtc ggcgtgacgg gcgcgaaaac cgagatcctg
    13861 ctcggcgggc cggccgcaag gcgagcggag caggaccttg ccggaacgct cggcctgaat
    13921 gcgcgcttct acagtgaaga gatcggcaag gcatcgatgt tcaagatgct gcgcagcgtc
    13981 ttctccaagg gcatggaggc gctcctcatc gagtttctgg cggccggcga acgcgccggg
    14041 attcgcgacg acctgtggaa agaagtcacc ggactgtttg cgacccagcc gttcgagaag
    14101 gtggcggcga actgggtgcg ctcccatgcg tccgcgcatg agcgccgcta ccacgaggtc
    14161 aaacaggttg ctgccgtgct gcgcgcgttg ggcatcgagc ccaccatcac cgcggcgacc
    14221 gaggcgttct tcgcccgctc ggcgagcctc ggtcttgtcc ccgccgcgag catggacgag
    14281 gtcgtgcgcc gtttcaacga gaggctatga agaagaaccg catgaaggaa aagctgcgcc
    14341 gcggggaggc ggtgttcggc gtctcggtga tgattccatc gccgcagatt gtggagatga
    14401 tcgccgcggc cggcttcgac tgggtgcttc tcgattgcga gcacggcacg ctcacgctcg
    14461 agagcgtgga gctcatggcc atggccgcgg aagcgagcgg catcacggca atcgcgcggc
    14521 cggtatcgcg cgcgccggag cacatcctgc aggtcctcga ccgcggcgtg atgggcgtcc
    14581 aggtcccgca cgtcaacacc ggcgccgatg cgcgcagagt gcttgaagcg gtcaagtacc
    14641 acccgctggg attgcgcagc ctggccgccg gcacgcgagc ggcggtgtac gactcgcacg
    14701 gtacgctcgc cgactatgta gcggccgcga ataatgaaac gctggtcgcg gtgcagctcg
    14761 aagaccgcga agccatcgac aatatcgatg agcttctaaa ggtagacaac atcgatgtct
    14821 tctttatcgg accttcggat ctctcgcagt ccatgggcca tcctggcaat ccgaaggcgc
    14881 acgctgtcgc cgaggcgatc gactcgagct ttcgcaagat gatcgccgcc ggccgcaccc
    14941 cgggaacgcc ggccaccgcg gacaatttgc ggggcgtgct cgacaagggt gtgcgctata
    15001 tctacaccca tcttccccgc ctgctttcat ccagtgcaaa gagctacctc ggccatgcgc
    15061 gcgcttaaac gcttcctgct cgtcttcagc ctgctgccga cgctggttgt cgcgcagggg
    15121 tatcccacga aagcggtcaa gatcatcgtt cccgcgcaac cgggcggcgg cctcgacctt
    15181 attgggcgca ccgtcggtgc cgagctgggc aagatgttcg atcagcagtt cgtcatcgag
    15241 aacgttgggg gaggcggggg ctccatcgcc tgccagcaga ctgcgcgcgc ggcgcccgac
    15301 ggctacacgc tgatggtcgg gtacgtcgcc acgcatgcga cgagtccggc cgtacgcaag
    15361 gtgccttatg acgcgatccg ggatttcacg ccgatcgcta tggtcggcgg cacgcgcaac
    15421 atgctcgtcg tccaccccaa cgtaccggtc tcgaacctga aggagttcat cgactacgcg
    15481 agaggcaaga cgggcaaggt gacgttcggc gcgggtggca tcggcaccct gaatcacctc
    15541 ctgatcgagc agctcaaggg cgcggctggc ttcgacgccg tgatcgcaca gtacaaaggc
    15601 atcgggccgg ctttcgccga cctcttcggc gggcagctcg aggcgcttac gccgggcgtg
    15661 gccgccgcac tgccccatat ccgcaacaag cgcgtgaagc cgatcgcggt gaccgggctt
    15721 acgcgtaacg cgctgctgcc tgaggtgccc accttcgagg aagccgggtt caaagggttc
    15781 gacggcgtac agtggtacgg catcgtcgga ccggcgaagc ttcccaccgc gatcgtggag
    15841 cggctgaacg cagagatcgg caaggcactc gcatcgcctg cgctccaaca acgtctggtg
    15901 gccgaagcga ttgaaccgat gcccatgacg ccgaacgaat tcggcgcctt catcaaggcc
    15961 gagctcgagc gctataccgt acttgcgcgc gaccgtaaca tccggctgga cgagtgaatt
    16021 ccgatcgaca tgccgcacgg cagcaacgtc ctcgacgcct ggggtgcgtc gcagactaat
    16081 cggcagtagt catgaccacg ggcgcgtggt cgctggttcc aaagtccgct tggaccatgc
    16141 agctctgcgc gcgcaccgcg aggcccgctg aagcaaggat gtaatcgagc cgccagccga
    16201 tgttgcgctg gcgcatgttg cgccagggcg cccaccaggt gaaaagaccg gcgttgtgcg
    16261 ggtcgagctt tcgcccgacg tccaccagtc cctgattcag aagctccgtg aagagcgcgc
    16321 gttcctcggg ccgttgcccg acgagctgcg gctttcgctc gcgcggatgc acgtccatct
    16381 cggtgtgcgt gatgttcatg tcgccgcaca agatgagctc gcgaccctgg tcgtgctgct
    16441 cgcgcaccca ggcgatgagc cgccggacga aatcgatctt cgccgggtaa tccttgttgc
    16501 cgttcggcac gtagatcgaa gcgatcagga gctctccaag ctgggcctgg acgatgcgcg
    16561 actccatgtc gaacgcgggg tggctgaagg ccgggggcgt ctcaaacgcg cctttgcgga
    16621 tatgcaacga gacgccggag taggcgcgca tgccgtgcca gaaagtatcg tagtccacga
    16681 ccttgcactg ctcggggatc tgcgtcggct cggctttgag ctcctgcagg cagaccacgt
    16741 ccggatgctc gcgcgcaacc cattcgcaga actgcgcctg gcgtgcacga atgccattca
    16801 cgttccaggt tgcgatcttc atgggcgaag cattgtagga tttccggcgt gaagcgcatc
    16861 gccgatctgc cgggcccgcg cgcgctgccg atcctcggca acctgctgca gatcgacaag
    16921 gccagcctgc atctcatcgc ggagaagtgg agccggcagt acggcgaagt ttttcgtttt
    16981 cgcatcgggt gccggcagct cgtcgccctc gccaatcccg aaaccatcgc cgccgtgctg
    17041 cgcgagcggc ccgacggctt tcatcgcacc gagcgcctga gcgccaccgc gcgcgagatg
    17101 gggttcaacg gcgtgttctc cgcgaacggc gacgcttggc gccgacaacg gccgatggtg
    17161 atggccggct tcgatccggc gcacatcaag agctacttcc cgatgctggt gaacgtcacc
    17221 gagcgcttct cgcgccgctg gcagcgtgcc gcggccgcgg gtacggcgat cgacctgcag
    17281 gccgatctca tgcgctacac ggtggacgtg accgcggggc tcgcttttgg ctcggacatc
    17341 aacacgctcg aatccgaaga ggacgtgatc caggcgcacc tggacaagat cttccccgcc
    17401 cttttcaagc gcttgctcgc acctgtgccg tactggcgat ggtttcccga ccgcaaggtc
    17461 gaacgtcatg tcgtggcgct gcgccgtgcg gtggagggtt tcatcgcgca ggcgcgtgcg
    17521 cgcatgagca atcctccgcg caacctgatc gaagccatga tcgccgcgcg cgacacgcca
    17581 ggcagcggcc tcacggacga cgacgtcgcc ggcaacgtgc tcacgatgct actggccggc
    17641 gaggacacca cggccaacac gcttgcctgg atgatttacc tcttgcatcg gcacccagag
    17701 gcgctcgccc gggcgggcga ggaggtttcg cgcgtgcgcc tggacaggta cgaggacgtt
    17761 tcggcgctgc ccttcgtcga tgcgtgcatc aacgagacca tgcgcctgaa gcccgtggcg
    17821 ccgatcatca tggcgcagcc cacgcgcgat accgagatcg cgggtgtgca ggtgccaaag
    17881 aagacgatcg tcatgtgcct catgcgggcg ggcgcgatcg acgagcggcg cttcgccgat
    17941 gccaaagcct tcgaacctgg gcgctggctc tcgggtgcgc aggcgagctc gcccaagcgc
    18001 gtgtccatgc cctttggcgc cgggccgcgc ctgtgtcccg ggcgttacct cgcgattctc
    18061 gagatgaaga tggtgatggc gatgctgctg agcaacttcg ggatcgagag cgtcggcacg
    18121 cccgacggcg gcgaggcgcg cgagcacctc gcgttcacca tgtccccggt cgggctgaag
    18181 ctgaagctca ggtcaggagc gacggcgaag gcttcaactc tcctcgctcg atccgcgtaa
    18241 cgagcgcggc gagcttctcg tggctcggcg ccgccacgcc gacctccttg cccttgcgcg
    18301 cgatgaagcc gttcatcgcg tcgatctcgg tgcgccgtcc cttgaggatg tcctgcgcca
    18361 tcgacgggcg ctggatgtcg ccgcgcggat tggcgccttc cttggggatc atcgcggcct
    18421 cgacttccgc cagcgccgcc ttgtcgcctt ccgcggcgcg cgcgaacttc tcgggctcga
    18481 gcgcgcgcac cttctccagc gtatagccga gcgcctggcc gacgcggatc gcctcgccgg
    18541 cgagcgcgat ctggaagcgg cggatcttct cgttcgcgag gcacgcgggc gaggtcaggc
    18601 cggtcgcggc ggtgacgccg ttgcccatgg agttctgcac cagcttcgac cagcgctcgc
    18661 cccacaggtt ggtggtggcc ttcgagctgt cgatgccgct gaaccacttc accagctctt
    18721 cgacgcgcgg cgtgatgcgc ccgtgcggct cgccaatgcg gaagatggtg tacttgtcgc
    18781 cgcctttcgg catggtgcgg cggatgcggc cggcctcgta caaatccacc gagatcatcg
    18841 acgccaccgc gccgacggtg cgtccccagc cgaccacgcc ggcaatggtc tcttcgttca
    18901 t
//
LOCUS       DASTPD010000332         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_388853, whole genome shotgun sequence.
ACCESSION   DASTPD010000332 DASTPD010000000
VERSION     DASTPD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388853"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFR57_11220"
     CDS             complement(<1..260)
                     /locus_tag="VFR57_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259973.1"
                     /translation="MRASIVFAAILLAACHGPPKRDVFIPPYAEKGCWAQFYELADFG
                     APMHQVEGPIYVEAIPGSIVLVPDLEQILPQPLFEEARSLIVG"
     gene            472..1005
                     /locus_tag="VFR57_11225"
     CDS             472..1005
                     /locus_tag="VFR57_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0259974.1"
                     /translation="MSGQLLAPMVRAIDRFAQLCGVVIAWLMVPLVAAVVYEVVARYA
                     FAAPTIWSFDVTYMLYGAMFMLGAAYALRIGAHIRTDFFWERWSLRTRGVIDSVAYIV
                     FFFPGIALFLWVGWDEAWYAYEIGELSEQTPWRPALWPLKACVPLAAALLLLQGVSEL
                     TKSLHAAITGRAFESGT"
     gene            1002..2426
                     /locus_tag="VFR57_11230"
     CDS             1002..2426
                     /locus_tag="VFR57_11230"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0259975.1"
                     /translation="MTYETAIGLTMLAVMIAAIFLGFPISFTLLFLAFAFGWWGLGDV
                     VFNLAYFQTIGLMKESLLAAVPLFIFMGFIAEQAGLMERLFAALRLLLAPVRGSLFIV
                     VIVTAAAFAMATGIVGAAVTVLGIMAAPIMIRSGYDGQLAAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYAAAIVPGFMLAGLYVIYVAVRSFFNPKLGPALPKELRAPSV
                     WSALGAFAVGVLPLAGLVAATLGSILAGLATPTEAAGMGAAGALVLATAYGKLTWERF
                     QRALMQSLATSSMVLVLALTSNIFGAVFARLGAADWITETLVSLPLPPGLMLALVTVL
                     IFLLGWPFEWPAVILVFLPIFYPVVIELGVDLVWFGALVAVTLQTAYLSPPVAMSAYY
                     LKQVAKGWSLLTIYKGMFQFMGIQVVAIALIFAFPAIATWLPDYLFRSGALVVSEPPT
                     SSSETYGESYEQMYQRSVESLKAQ"
     gene            2599..2697
                     /gene="ffs"
                     /locus_tag="VFR57_11235"
     ncRNA           2599..2697
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR57_11235"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2714..4222
                     /gene="dnaX"
                     /locus_tag="VFR57_11240"
     CDS             2714..4222
                     /gene="dnaX"
                     /locus_tag="VFR57_11240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0259976.1"
                     /translation="MSYQVLARKWRPRDFASLVGQEHVVRALRHALEHKRLHHAYLFT
                     GTRGVCKTTLARILAKCLNCETGITPQPCNTCSACVEIDAGRFVDLLEVDAATNTKVD
                     EMRQLLDTAQYAPTRGRFKVYVIDEVHQLSGHAFNAMLKTLEEPPEHMKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPREAIAEHLGNILGREGIKYEPGALALLARAAAGSMRDAL
                     SLLDQAIGHGGGAVTAASAAEMLGAIDQGHLVRLIEAIAADDAAAAVKIADDMQARSL
                     SFDSALTELASLLLQIALAQSVAGAVHDARVKELATRLDPESVQLYYQIALQGREDLP
                     LAPDEHGGFVMTVLRMLAFRPEGAGRDLPVPKKTENAQARTQSSDWTALVQQLPVTGA
                     ARELARNAELRRRDGASFELVVPKAKAYLAERSYLEKLKAALEQQLAAPVSLKVAVGE
                     VAGASVASLEAGEREARRTAAAQAVASDGFVQDLVDLFDGKVVDSTIREDRK"
     gene            4230..4553
                     /locus_tag="VFR57_11245"
     CDS             4230..4553
                     /locus_tag="VFR57_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0259977.1"
                     /translation="MKGNIGQLMKQAQQMQESMRRMQESLATTEVEGQSGAGMVKVQM
                     TCKHEVKRVTVDPSLVGDDREMLEDLLAAAFNDAARRVEATIAEKMSGMTAGLGLPPG
                     FKLPF"
     gene            4550..>4941
                     /locus_tag="VFR57_11250"
     CDS             4550..>4941
                     /locus_tag="VFR57_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU0259978.1"
                     /translation="MSKPPVPSAKGLERLVQALRVLPGVGPRSAQRMAFHLLQHDRGG
                     ADELARALSEALASVRRCDQCNNFSEEEVCALCRSPRRDRSLLCVVETPADLAMVEQT
                     LSYSGMYFVLMGRLSPLDGVGPRDIGLDR"
BASE COUNT          789 a         1595 c         1652 g          905 t
ORIGIN      
        1 ccgacgatca ggctgcgcgc ctcttcgaaa agcggctgcg ggaggatttg ctccagatcg
       61 gggaccagga cgatcgagcc gggaatggcc tccacgtaaa tgggaccctc gacctggtgc
      121 atcggcgcac cgaaatcggc cagctcgtag aactgcgccc agcagccctt ctcggcgtac
      181 ggagggatga agacgtcgcg cttgggcggc ccgtggcagg ccgccagcag gatggcggca
      241 aagacgatcg atgcacgcac gccggccatg atgcccgcat gccgatcaat tcgcaaaccg
      301 atgggcgggc ggccgtgcag ccgttcatgt gaacaggaaa ctctcgtttc cccaaccagg
      361 agaccacacg atgtccgcgc gacacggtgc ccgcttgaac acagcatgcg gggcaaatcc
      421 ttaaggtgcg cttaaagctg gcttaagtct gccgcttcta ggctcggcgc catgtccggg
      481 cagctcctgg cgccgatggt ccgcgcaatc gaccgcttcg cccagctttg cggcgtggtg
      541 atcgcctggc tcatggtgcc gctggtcgca gccgtggtct acgaggtcgt cgcgcggtac
      601 gccttcgccg cgccgaccat ctggtcgttc gacgttacct acatgctgta cggcgcgatg
      661 ttcatgctgg gcgccgcgta cgcgctgcgt atcggggcgc atatccgcac tgacttcttt
      721 tgggagcgct ggtcgctgcg cactcgcggg gtcattgact cggtcgctta catcgtcttc
      781 ttctttcccg ggatcgcgtt gttcctctgg gtgggctggg acgaagcttg gtatgcctac
      841 gagataggcg agctctcgga gcagacgccg tggcgaccgg cgctctggcc gctgaaggcg
      901 tgcgtgccgc ttgccgcggc gctgctgctc ctgcaaggcg tttccgagct gacgaagagc
      961 ctgcacgccg caatcacggg ccgcgccttt gagagcggca catgacctac gagaccgcca
     1021 tcgggttgac gatgctcgcc gtaatgatcg cggcgatctt tctcggcttt cccatctcgt
     1081 tcacgctgct gttcctggct ttcgccttcg gctggtgggg gctgggcgac gtcgtgttca
     1141 acttggcgta tttccagacg atcgggctca tgaaggagtc gctgctcgcc gcggtgccgc
     1201 tcttcatctt catgggcttc atcgccgagc aggccgggct catggagcgc ctattcgccg
     1261 cgctgcggct gctgctcgcc ccggtgcgcg gatcgttgtt catcgtcgtc atcgtcaccg
     1321 ccgcggcttt cgccatggcg accggtatcg tgggcgccgc ggtcaccgtg cttggcatca
     1381 tggccgcacc gatcatgatt cgctcgggct atgacggcca gctcgccgca ggggcgatca
     1441 ccgcgggcgg tacgctgggc atcctcattc cgccttcggt gatgttgatc gtgatggggc
     1501 cggtgctcgg cgtatccgtg gcagacctct atgccgcggc catcgtcccc ggcttcatgc
     1561 tcgccgggct ctatgtcatc tatgtcgccg tccgcagctt cttcaacccg aagctgggcc
     1621 ccgcgctacc caaggagctg cgcgcaccgt cggtgtggtc ggcgctcggc gccttcgcgg
     1681 tcggcgtact gccgctcgcc ggactggtgg cggctacgct gggctcgatc ctcgccgggc
     1741 tcgcgacgcc gaccgaggcc gcgggcatgg gcgccgccgg tgcgctcgtg ctcgccacgg
     1801 cgtacggcaa gctgacctgg gaacgcttcc aacgcgctct gatgcagtcg cttgcgacga
     1861 gcagcatggt gctcgtgctc gcgctcacct cgaacatctt cggtgcggtg ttcgcccgtc
     1921 ttggcgccgc ggattggatc accgaaacgc ttgtctccct gccgttgccc cccgggctga
     1981 tgctcgcgct ggtaacggtc cttatattcc ttctcggctg gccgttcgag tggccggcgg
     2041 tgatcctggt tttcttgccg atcttctatc cggtggtgat cgagctgggg gtagacctcg
     2101 tgtggttcgg ggccctggtc gccgtcacgc tgcagaccgc ttacctctcg ccgccggtgg
     2161 cgatgtcggc ctattacctc aagcaagtgg cgaaaggctg gtcgcttctg accatctaca
     2221 agggcatgtt tcagttcatg ggcatccaag tcgtcgccat cgccctcatc ttcgcctttc
     2281 cggcgatcgc cacctggctt cccgattatc tgttccgcag cggggccctt gtggtgagcg
     2341 aaccgccgac ctcgtcgtcc gagacctacg gcgagtccta cgagcagatg taccagcgct
     2401 ccgtcgagag cctaaaggct cagtaggggc gcacgaagaa aaacgcccgc agggcttgcg
     2461 cccgcgggcg ttgaagccat cgcgactatt gcgatggagg aggatacgtg agcagcgtag
     2521 cgtcggcttc caacgatccg ctgacttcaa gctgacaacg ggcttactga cccggccgtt
     2581 tcaattagaa ttgatcccgg cgggcctccc cgcattgcag tctggtgaac ctggtcaggt
     2641 ccggaaggaa gcagccacag ccggtcgctg cgagtgccgg gggtctggct cgccacagaa
     2701 ttcgattcga gcgatgagct atcaagtcct tgcccgcaag tggcggccgc gggatttcgc
     2761 gagccttgtg gggcaggagc atgtcgtgcg cgcccttcgc cacgcgctcg agcacaagcg
     2821 gctgcaccac gcctatcttt tcaccggcac gcgcggcgtg tgcaagacca cgctggcgcg
     2881 cattttggcg aaatgcctga actgcgagac cgggattacg ccccagccgt gcaacacctg
     2941 ctcggcgtgc gtcgagatcg acgccgggcg cttcgtcgat ctgctcgagg tcgacgcggc
     3001 gacgaacacc aaggtcgacg agatgcgcca gctgctggac accgcgcagt acgcgccgac
     3061 gcgcggccgc ttcaaggtct acgtgatcga cgaggtgcac cagctctcgg ggcatgcctt
     3121 caacgccatg ttgaagacgc tggaggagcc gcccgagcac atgaagttca tcctggcgac
     3181 taccgatcct cagaagatcc cggtgacggt gctcagccgc tgtctgcagt tcaacctcaa
     3241 gcagatgccg cgcgaagcca ttgccgagca cctggggaac attctaggtc gggagggcat
     3301 taagtatgag ccgggagcgc tcgcgctact cgcgcgtgcc gcggcgggca gcatgcgcga
     3361 tgcgctgtcg ctgctcgacc aggcgatcgg gcatggcggc ggcgcagtca cggcggcgag
     3421 cgccgcagaa atgctcggcg ccatcgatca gggtcatctc gtgcggctga tcgaggcgat
     3481 cgccgcggac gatgccgccg ccgcggtgaa gatcgccgac gacatgcagg cacgcagcct
     3541 gtccttcgac agcgcgctta ccgagctggc gagcctgctc ttacaaatcg cactggcgca
     3601 gtcggtcgcc ggcgcggtgc acgacgcgcg cgtgaaggag ctcgcaaccc ggctcgatcc
     3661 ggagtcggtg cagctctact atcagatcgc gctgcagggc agggaagacc tgccgctcgc
     3721 gcccgacgag cacggcggct tcgtcatgac ggtgctgcga atgcttgcgt tccgtcccga
     3781 aggcgccggc agggacttgc cggtcccgaa gaaaacggaa aacgcccagg ctcgaacaca
     3841 aagtagcgac tggacggcgc tcgtgcagca gttgccggtc acgggggccg cgcgtgagct
     3901 tgcgcgtaac gccgaattaa ggcgtcgcga cggtgcgagc ttcgagctcg tggtgccgaa
     3961 agccaaggcc tacctcgccg agcgcagcta cctggaaaag ctcaaggcgg cgctagagca
     4021 gcagctcgca gcgccggtga gcctgaaggt cgcggtcggg gaagtcgccg gcgccagcgt
     4081 ggcgtcgctc gaggcgggcg agcgcgaagc ccgccgcacc gccgccgccc aggcggtggc
     4141 gagcgacggc ttcgtgcagg acctggtcga tctcttcgac ggcaaggtcg tcgattccac
     4201 catccgggaa gaccgaaagt gagccgctca tgaaaggtaa cattggccag ttgatgaagc
     4261 aggcgcagca gatgcaggag agcatgcgcc gcatgcagga gagcctcgcc accaccgagg
     4321 tagaggggca atcgggcgcc ggtatggtga aggtgcagat gacctgcaag cacgaagtga
     4381 agcgcgtcac cgtcgatccc tcgctcgtcg gcgacgatcg cgagatgctg gaagacctgc
     4441 tcgccgccgc gttcaacgat gccgcgcgcc gggtcgaggc tacgatcgcg gagaagatga
     4501 gcgggatgac cgccgggctc ggactgccgc cgggcttcaa gttgccgttt tgagcaagcc
     4561 gccggtcccg agcgcaaaag gactggaacg gctcgtccag gcgttgcgcg tgctgcccgg
     4621 cgtggggccg cgctccgcgc agcgcatggc tttccatctt ctgcagcacg atcgcggggg
     4681 cgccgacgag ctcgcccggg cgctctcgga ggcgctcgcg agcgtccgcc gctgcgatca
     4741 atgcaacaac tttagcgagg aggaagtttg cgcgctgtgc cgatcgccgc ggcgcgaccg
     4801 ctccttgctg tgcgtcgtgg agacgcctgc ggaccttgcc atggtcgagc agacgctcag
     4861 ctactccggg atgtatttcg tgctgatggg ccggctctct ccgctcgacg gcgtcggccc
     4921 gcgcgacatc gggctcgatc g
//
LOCUS       DASTPD010000333         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_388880, whole genome shotgun sequence.
ACCESSION   DASTPD010000333 DASTPD010000000
VERSION     DASTPD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388880"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR57_11255"
     CDS             <1..406
                     /locus_tag="VFR57_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0259979.1"
                     /translation="ERLRAQVLQREFKRGGELQVLLLRYTMALITQMSQTALCYRHHS
                     AEQQLCRWLLMSLDRLPGNELVMTQELIADMLGVRRQSVTEAARKLQADGLIRYARGR
                     ITALDRPALEQRVCECYTVVKKEYERLLSNGS"
     gene            complement(427..1158)
                     /locus_tag="VFR57_11260"
     CDS             complement(427..1158)
                     /locus_tag="VFR57_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0259980.1"
                     /translation="MSVAGNGELVLFLHGIRGNRRNWRGQIEFFSRGFKAAACDARGY
                     GDSEDYGGPLQFEYFTGDVLRIAEHFKARKLHLVGLSMGGRIARNVALRAPERLHSLV
                     LISTNPGFDTLSTERVMQFINERRSRTPQTLQTLLGSGANRAAYEELLDSVSRIHEAS
                     YQKALEASVAQDRGAPIEQIRVPTLVIAGEEDTVYPPELAEEMARRIPSAKLLMFERT
                     GHLANLEQPEKFNQAVFDFISRHNR"
     gene            complement(1201..2154)
                     /locus_tag="VFR57_11265"
     CDS             complement(1201..2154)
                     /locus_tag="VFR57_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0259981.1"
                     /translation="MKLLAGMFLGLACFAAHAWPTKPVRIVVAYPPGGGIDVLARQLA
                     DKLSPAWGQPVVVENRPGANTIVATDAVAKSGADGHTVLMTTDATFSINPHLYAKLAY
                     DAERDFTPVTMLVLLQQLLVAHPALPANTLEELIKLAKAKPGLINYASYGSGSQPHLA
                     GEMLKNKASIDLVHVPYKGISLAVPALIAGEVQLTFAGIATSMPQLKAGRIKALATGG
                     AARSPLLPQVPTFAELGYPEVETHAWFGLFLPAGSPAQAVERIYRDSKKILEEPEFRL
                     KQLVEKGYDPVGSAPAEFVIYLKKDREARGRAVRISGAKAE"
     gene            2180..3385
                     /locus_tag="VFR57_11270"
     CDS             2180..3385
                     /locus_tag="VFR57_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259982.1"
                     /translation="MFLNMKLLAQHTLNGFGGMGEGMSMQLARDGRRVLWLAHESAPK
                     NFTAVDVTEPRSPKVVVQTDLPHAQMRSNSLEVSGELMAVAYQVSKFGLKPAGFELFD
                     ISVPERPKSISFFDASSPHSRGCHQLWFVDGKTVHMACADPELKPRNPKDDQVYRIVD
                     VANPSKPVAVGRWHLPGTMEGDDAPPPERLPAQFDAGFRAHNTNVYPERPDRCYLAYI
                     DGGVMVLDISDRARPKMVSRWTNSPPYNGFNHTALPFFGRGLLVVSDESVKDKAVDHP
                     KLVWLLDARDERRLVSIATLPMPPVDAFKNRGGRFGAHNLHENPPVPGAWRSEDVVLG
                     TFFNGGVRAYDVSDPYSPREVAYFVPPPPERSKTGAAQLNDVFVDDRGIVFTVDRGGG
                     GLYILEMKL"
     gene            3382..4308
                     /locus_tag="VFR57_11275"
     CDS             3382..4308
                     /locus_tag="VFR57_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0259983.1"
                     /translation="MSVDGRTPVVVVTGFLGSGKTTLVNQLLKREPESLVIVNEFGEV
                     PVDHLLVESSSEKAVALPNGCLCCHLRGDLEETLIGAAMRRRRGEIAGFDRVIVETSG
                     LADPGPVAQTLYADAALGRDYKLGSIVTVVDAADTAARSISPEIARSQIAAADLVVLS
                     KIDRAANTTEAEAEVRALNPGVQCIAARYGEVDPGAILEVHPARQPIVRPVAHSGAIR
                     SFVLKFESPVARTLLEQFIATLVELRGADLLRVKGVVPLKEGASVLVQGVRHVFDRLR
                     PIERGEPSLVFVTHRIEKAAIEALWAAMARLS"
     gene            complement(4305..4556)
                     /locus_tag="VFR57_11280"
     CDS             complement(4305..4556)
                     /locus_tag="VFR57_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259984.1"
                     /translation="MAKTGDLREALAALQKGDWQAAHAIVQADEDSRLACWAHGIVHL
                     MEGDPANARYWYRKAGRPFGEDVAAEVATLSEALKSRKG"
     gene            4606..5142
                     /locus_tag="VFR57_11285"
     CDS             4606..5142
                     /locus_tag="VFR57_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0259985.1"
                     /translation="MARPRKKAPPLTVSRAALLARGSDDEFRRLIHDLIAYGHKLDAC
                     RDAFAAIAGISGAQYEILMLVSRAEGLSIGEVAARMHRSGAFITIEANKLVGAGILEK
                     ASDPADGRRVLLRMNEKSVELLERMAPYQRRVNDVLFEPLDAKRFRELRTLAAELVAC
                     GDRAVAMLELLLHEAEAA"
     gene            5139..>5438
                     /locus_tag="VFR57_11290"
     CDS             5139..>5438
                     /locus_tag="VFR57_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEU0259986.1"
                     /translation="MRPKQHLEFVKVDLDSGWEVPPGYPPGFYQKILASDLDEKKKTG
                     SRTRLLKIDPGAFSTEPFVHDHWEEVYLVQGDLVVGNDKQGKGGTQCFAPTYACRP"
BASE COUNT          916 a         1803 c         1800 g          919 t
ORIGIN      
        1 cgagcgcctt agagcgcaag tcctgcagcg ggaattcaag cgcggcggag agctgcaggt
       61 tctcctcttg cgttacacca tggccctcat cacgcagatg tcgcagaccg cgctctgcta
      121 ccggcatcac tcggccgagc agcagctttg tcgctggctg ctgatgagcc tggaccgctt
      181 gcctggaaac gagctggtga tgacgcaaga gttgatcgcc gacatgctgg gcgtgcgccg
      241 ccaaagcgtc acggaagccg cccgcaagct gcaagcagat gggttgatcc ggtatgcgcg
      301 cgggcgcata acggcgctgg atcggcccgc gctcgagcag cgggtctgcg aatgctacac
      361 cgtggtcaag aaggaatacg agcgacttct aagcaacggc tcctagaggc ccacgctcca
      421 agagggtcag cggttgtgcc gggagatgaa gtcgaaaacc gcctggttga acttctcggg
      481 ctgctcgagg ttcgcgaggt ggccggtgcg ctcgaacatg aggagcttcg cgctgggaat
      541 gcggcgcgcc atctcctcgg cgagctcggg agggtagacc gtgtcttcct cgccggcgat
      601 caccagcgtg ggcacccgga tctgctcgat cggcgcgccg cggtcctgcg cgaccgaggc
      661 ttcgagcgcc ttctggtacg aggcttcatg gatgcgcgag acgctgtcga gcagctcctc
      721 gtaggcggca cggttcgccc ccgagccgag aagcgtctgc agggtctgcg gcgtacgcga
      781 gcggcgctcg ttgatgaact gcatgacgcg ctcggtggag agcgtgtcga atcccgggtt
      841 ggtgctgatt agtacgagcg aatgcagccg ctccggggcg cgcagcgcca cgttgcgcgc
      901 gatgcggccg cccatggaca ggccgacgag gtggagcttg cgcgccttga agtgctcggc
      961 gatgcgcaga acgtcaccgg tgaaatactc gaactgcagc gggccgccgt agtcctcgga
     1021 atcgccgtag ccgcgcgcgt cgcaggccgc ggccttgaag ccgcgcgaga agaactcgat
     1081 ctggccgcgc cagttacgcc ggttgccgcg gatcccgtgc aggaaaagca ccagctcgcc
     1141 gttgccggcg acggacaccg caagccgcgg tgcaggttcg acgaaaaccg tgtccagggt
     1201 tcactccgcc tttgctcccg agattctgac ggcgcggcca cgcgcctcgc ggtctttctt
     1261 taaataaatg acaaattctg cgggcgcgct cccaaccggg tcgtacccct tttccacgag
     1321 ctgcttcagc cggaactccg gctcctcgag gatcttcttt gagtcgcggt agatgcgctc
     1381 cacggcctgc gcaggcgagc cggccgggag gaaaagcccg aaccaggcgt gcgtctccac
     1441 ttccggatag cccagctcgg cgaaggtcgg tacctgcggc agcagcggcg agcgtgccgc
     1501 gccgcccgtc gcgagcgcct tgatgcgccc ggccttgagc tgcggcatcg aagtcgcgat
     1561 cccggcgaaa gtgagctgca cctcgcccgc gatcagcgcc gggactgcga gcgaaatccc
     1621 cttgtagggg acatgcacga ggtcgatgct cgccttgttc ttgagcatct cgccggcaag
     1681 gtgcggctgg ctgccgctgc cgtacgaggc gtagttgatc aagccgggct ttgctttggc
     1741 cagcttgatc agctcctcca gcgtgttagc gggtagggcc ggatgtgcga cgagcagctg
     1801 ctgaaggagg acgagcatcg tcaccggcgt gaaatcgcgc tcggcgtcgt atgcgagctt
     1861 ggcgtagaga tgcgggttga tggagaacgt ggcatcagtg gtcatcagca cggtgtggcc
     1921 atcggcgccg gacttcgcga ccgcgtcggt ggcgacaatc gtgtttgcgc ccgggcggtt
     1981 ctccaccacg accggctgtc cccatgcggg cgagagcttg tcggcaagtt gccgcgcgag
     2041 gacgtcgatg ccgccgccgg gtgggtacgc gaccacgata cgcaccggtt tcgtgggcca
     2101 ggcgtgggcg gcgaaacaag cgagcccaag gaacattccg gcaaggagct tcatggcggg
     2161 cagatgatat cttaggggca tgttcctcaa catgaagctg cttgcgcagc acacgctcaa
     2221 cggcttcggc ggcatgggcg aaggcatgtc gatgcagctc gcgcgcgacg gccgtcgcgt
     2281 gctatggctc gcgcacgaga gcgcgcccaa gaacttcacc gcggtcgacg tcaccgagcc
     2341 gcgctcgccg aaagtcgtcg tgcagaccga cctgccgcac gcccagatgc gttcgaactc
     2401 gctcgaggtc tcgggcgagc tcatggcggt ggcctaccag gtgagcaagt tcgggctgaa
     2461 gcccgccggc ttcgagttgt tcgatatctc cgttccggaa agacccaaat ccatctcatt
     2521 cttcgacgcc tccagcccgc actcgcgcgg ctgccaccag ctctggttcg tcgacgggaa
     2581 gaccgtgcac atggcctgcg ccgatcccga gctcaagccg cgcaacccga aggacgacca
     2641 ggtctatcgc atcgtcgacg tggcgaatcc gtcgaagccc gtcgcggtcg gccgctggca
     2701 cctgcccggc accatggaag gagacgacgc cccgccgccg gagcgccttc cggcgcagtt
     2761 cgacgccggg ttccgcgcgc acaacaccaa cgtctatccc gagcgcccgg accgctgcta
     2821 cctcgcgtat atcgacggcg gcgtcatggt gctcgacatt tccgaccgcg cgcggccgaa
     2881 aatggtttcg cgctggacca actcgccgcc gtacaacggg ttcaaccata ccgcgctgcc
     2941 gttcttcggc cgggggcttc tcgtggtgag cgacgagtcg gtaaaggaca aagccgtgga
     3001 ccaccccaag ctcgtctggc tgctcgacgc gcgtgacgaa aggcgcctgg tgagcatcgc
     3061 cacgttgccg atgccgccgg tcgacgcctt caagaaccgg ggcgggcgct tcggcgcgca
     3121 caacctgcac gagaacccgc cggtgcccgg cgcgtggcgc tcggaggatg tcgtgctcgg
     3181 cacgttcttc aacggcgggg tgcgtgccta cgacgttagc gatccgtatt cgccgcgcga
     3241 ggtggcgtat ttcgtgccgc ccccgcccga gcgctccaag acgggcgccg cgcagctgaa
     3301 cgacgtcttt gtcgacgacc gcggcatcgt attcaccgtc gaccgcggcg gcgggggact
     3361 ctacatcctc gaaatgaagc tgtgagcgtg gatggacgca cgccggtcgt cgtcgtaacc
     3421 ggcttcctcg gcagcggcaa gaccacgctc gtcaaccagc tgctgaagcg cgagcccgaa
     3481 tcgctggtga tcgtgaacga gttcggcgag gtgccggtcg accatcttct cgtcgagagc
     3541 tcaagcgaga aggcggtggc gctgccgaac ggctgcctct gctgccacct gcgcggcgac
     3601 ctggaagaga cgctgatcgg cgccgcgatg cgccggcgcc gcggcgagat cgccgggttc
     3661 gaccgcgtaa tcgtcgagac gagcggtctc gccgatcccg gccccgtggc gcagacgctc
     3721 tacgccgacg cagcgctcgg gcgcgactac aagctcgggt ctattgtcac cgtggtggac
     3781 gcagccgaca cggccgcccg cagcatatcg cccgaaatcg cgcgcagcca gatcgctgct
     3841 gccgacctcg ttgtgctttc gaaaatcgac cgtgccgcca ataccaccga ggcggaagcc
     3901 gaggtgcgcg cgctcaatcc aggggtgcaa tgtatcgccg cgcgatacgg tgaagtcgat
     3961 ccgggcgcca tcctggaagt tcatcctgca agacaaccca tcgtccgccc ggtcgcgcac
     4021 agcggcgcca ttcgcagctt cgtgctgaag ttcgagtcgc cggtggcgcg cacgctgctc
     4081 gagcagttca tcgcaacgct ggtcgagctg cgcggcgccg atctgctgcg cgtgaaaggg
     4141 gtcgtgccgc tgaaggaagg cgcttcggtg ctggtgcagg gcgtgcgcca cgtgttcgat
     4201 cgcttgcgcc cgatcgagcg cggcgagccc agcctcgtct tcgtcactca ccgcatcgag
     4261 aaggcggcaa tcgaggcgct ctgggctgcg atggcgcgac tttcttaacc ttttcgactc
     4321 ttcaaagctt cgctgagcgt cgccacctcc gcggccacgt cctccccgaa cggccgcccg
     4381 gccttgcggt accagtagcg cgcgttcgcc ggatcgcctt ccatcaggtg cacgatgccg
     4441 tgtgcccagc aggcgaggcg cgagtcttcg tccgcctgca ctatcgcgtg cgccgcttgc
     4501 cagtcgccct tctgcagcgc cgcgagcgcc tcgcgcagat cgcccgtttt ggccatttag
     4561 ctaattgaca aaggtaattg cccgggcgga gaattctcgc gctccatggc gcgtccccgc
     4621 aaaaaagctc caccgctcac ggtatcacgc gcggcgctgc tcgcgcgcgg ctcggacgac
     4681 gagttccgcc gcctcatcca cgatctcatc gcttacggcc acaagctcga cgcctgccgc
     4741 gacgccttcg ccgcgattgc cggcatctcg ggcgcgcagt acgagattct gatgctcgtg
     4801 agccgcgccg aggggctgtc gatcggcgag gtggcggcgc gcatgcaccg ctccggcgcc
     4861 ttcatcacca tcgaggcgaa caagcttgtc ggcgcgggga tcctcgaaaa ggcgtccgac
     4921 ccggccgacg gccgccgcgt ccttctcagg atgaacgaga aaagtgtcga gcttctggag
     4981 cgcatggctc cgtaccagcg ccgtgtcaac gacgtgctgt tcgagcccct cgacgcgaag
     5041 cgcttccgcg agctgcgcac actcgccgcc gagctcgtag catgcggcga tcgcgccgtc
     5101 gcgatgctcg agctccttct ccacgaggcg gaggccgcat gagaccgaag cagcatctgg
     5161 agttcgtgaa agtggacctg gacagtggct gggaagtgcc ccccggctat ccgccgggct
     5221 tttaccagaa gatccttgcg tccgacctgg acgagaagaa gaagaccgga agccgcacgc
     5281 ggcttctcaa gatcgatccg ggcgcgttct ccaccgagcc cttcgtccac gaccactggg
     5341 aggaggtgta ccttgtgcag ggcgacctcg tggtcggcaa cgacaagcag ggcaagggcg
     5401 gcacccagtg cttcgcgccc acctacgctt gccgcccg
//
LOCUS       DASTPD010000334         8270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1206942, whole genome shotgun sequence.
ACCESSION   DASTPD010000334 DASTPD010000000
VERSION     DASTPD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206942"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR57_11295"
     CDS             <1..573
                     /locus_tag="VFR57_11295"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU0259987.1"
                     /translation="KAGDQPEAKPVFDLNTRDLIATARRMRDEGRLPSGTEIERAPEL
                     FIGAADVPTDPKPGWIPAALLAKAQAGADFVQTQFCMDIGMVRRYAARLREAGLNLPV
                     LIGIAPIPSARSARWMREKLFGTIVPDEIVKRLEHAADPKREGRRICVELLQQLAEIP
                     GVAGAHVMAPMNFAEIPAAIEESGVLKRKR"
     gene            complement(570..833)
                     /locus_tag="VFR57_11300"
     CDS             complement(570..833)
                     /locus_tag="VFR57_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259988.1"
                     /translation="MALPLIKLSLAAAAGYFLYSSYVRSRAAGSTTNASDAVDDIDLP
                     ANPTVEEKLDAGVQETFPASDPVSISQPSETAYEAAQRRKPSN"
     gene            complement(818..1012)
                     /locus_tag="VFR57_11305"
     CDS             complement(818..1012)
                     /locus_tag="VFR57_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259989.1"
                     /translation="MERRPLDNIEPLGPDATADDALDQGLLETFPASDPIAVGLAYYR
                     RDGTKFARMPRKGDMTWPFP"
     gene            1070..1918
                     /locus_tag="VFR57_11310"
     CDS             1070..1918
                     /locus_tag="VFR57_11310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0259990.1"
                     /translation="MQLGFIGLGQMGRPMVERLKSAGHSLKVYNRTRSSPDIAAKADD
                     AVAGEVVITMLADDAAVRAVWLDTGLARRLPKGTIHLNMATVSMAISRELASIQKENY
                     VAAPVFGRPPLAAQGQLDIIAAGPKAALEYCQPLFSVLGKQTFVVGDDPAKANAVKIA
                     RNFLLACLIESLGEAFALVRRCGVAPADFLNVIGNTSLGSPAYRNYGKMIVEQAWLPA
                     QFAMPLGVKDVELALTTAREAGMRLRLGEMIRDHLLEAIAAGRAEEDWAALAGHIANE
                     AGLSER"
     gene            2002..2211
                     /locus_tag="VFR57_11315"
     CDS             2002..2211
                     /locus_tag="VFR57_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259991.1"
                     /translation="MDHTAKRALLEQAVRLLGRMKVAKRLGVPEPVLGAWMRGETPMP
                     TINLLILATMLDEQAKAQDGAVRPS"
     gene            2345..3214
                     /locus_tag="VFR57_11320"
     CDS             2345..3214
                     /locus_tag="VFR57_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0259992.1"
                     /translation="MKLPKDRVAYSAIVDRPPLKLPNDARVAVWTIVNVEEWSIERAM
                     PRMVLSPPQGQPLLPDLPNWAWHEYGMRVGFWRFIEVLEKFGLKATLAINGSVIKSYP
                     RVAEAALEAGWEFMGHGYVQRPMHHVEDQRDAIRKTVAEIKAFTGKKPRGWESPGLTE
                     TYDTIDWLAEAGIEYVADWVLDDQPVKIQTAHGPIVSVPYTVEMNDISMMVLQHHPSE
                     EWLRRGTDHFDRLYADGEKTARVMAISVHPYISGVPHRIAYLERLYEYIRQRPGVALW
                     TGEEILDWYLGKS"
     gene            3189..4325
                     /locus_tag="VFR57_11325"
     CDS             3189..4325
                     /locus_tag="VFR57_11325"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0259993.1"
                     /translation="MTGTWEKAEALLPVLKERAAKCEELRRLPDETLRDFHDARLFRI
                     HQPKRIGGEELEFAAVVTYGALFARACASTAWNWVNFAAHHMMLGMFPPKAQDEIWGA
                     SVDALIASSFVFPAGKARKVEGGYVLSGRWPFSSGVDPSEWNMLAGLARLDDHLPPEQ
                     RVFLLKRSQYKIIDTWHAGGLRGTGSNDVEVKEQFVPEHMTLAVADTKGGPTPGSAVN
                     PGVLYKLPVFALFPYMLSGVALGIAEGLIEGFNPGAGRMTGARVAEIQSTQIRLAEAT
                     AYARASRLVQLANCRDAEAQLPDIKTKARYRLEGAYAVDWAVRAVDVMFGLYGASGLY
                     ESSHVPRAFRDAHAVKQHFSFNTDIAGTTYGRVALGLASDNPTL"
     gene            complement(4277..4690)
                     /locus_tag="VFR57_11330"
     CDS             complement(4277..4690)
                     /locus_tag="VFR57_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259994.1"
                     /translation="MRTKRALLYAWLLATLFSGIPSTLYALLTGRDPLEATRAAGAMI
                     AMPNSLAAAALVHVSVSLFWALVLWLLLPYRRTVLWVMVASAAIAVLDLRVIAPALFP
                     EVAALYFWPQFADHMMWGACYSVGLSLARPSATRP"
     gene            4703..5116
                     /locus_tag="VFR57_11335"
     CDS             4703..5116
                     /locus_tag="VFR57_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0259995.1"
                     /translation="MRWTIVLPTVSGARQDAPTMKTFLLIVSLLLAPLLASAASPFTS
                     LHPEEAIPDPTEQPSPGPYSEFIGRVQQRLLVLGFGAGPVNGDFGAKTQAALAQFQLS
                     RAIPAGGQLDDQTLAELGVVREEAAAGASAEQSER"
     gene            complement(5172..5549)
                     /locus_tag="VFR57_11340"
     CDS             complement(5172..5549)
                     /locus_tag="VFR57_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259996.1"
                     /translation="MTLKTLIAIAVASAFAVPVQAAGDKAKDASASPSTSQSQSASQG
                     ASNTGNKGADAMFNSLDKNKDGMLTPEEAKGTPHDKDFATLDKNNDGKLSPQEHAAAP
                     EHAEKKAGAGGTASSDPKAPSKK"
     gene            5666..6103
                     /locus_tag="VFR57_11345"
     CDS             5666..6103
                     /locus_tag="VFR57_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU0259997.1"
                     /translation="MQSKLLLQSAERVFAVIFGTGDEPVAGLTAFAAQHKLRASSFTA
                     IGAFSEATLGYFDWEKKDYERIAVGEQVEVLALVGDIALQDGKPKLHAHVVLGGRDGN
                     ARGGHLLSARVRPTLEVIVTESPSYLTRRHDPQSGLALIDIRA"
     gene            6176..6847
                     /locus_tag="VFR57_11350"
     CDS             6176..6847
                     /locus_tag="VFR57_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0259998.1"
                     /translation="MKLNAIAVAVAAALLSFGAVAQEKKQESQAGQSSPSMSQSQGAS
                     GSSSTQSPSAAGGASSSSSSDKSASGSAGASSDKSSQGGSAAAGASSGGQAKSESKGG
                     SAAAGSGQQQHDAETIKQVQQKLNVTADGIMGPQTQKALQDFQQQKGLDANGQLDQKT
                     LSALGVSGSAAGGASGKSSSSASSSSSGSSMGSGGSSASGSTTEQKPQQAPAPTSPST
                     SEKKQ"
     gene            6930..7310
                     /locus_tag="VFR57_11355"
     CDS             6930..7310
                     /locus_tag="VFR57_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0259999.1"
                     /translation="MRPASHSEVETLLKKEARSRLPLLRQIFIYLDPFALFKDASRGT
                     AQARALAVSYNRAMRWMLVPYIRRWLAIAASLGLMIAPAEAAAAQQAAFAIPMAAIAV
                     ACCIAITIALVTVAVYLLLGNKVV"
     gene            7345..7758
                     /locus_tag="VFR57_11360"
     CDS             7345..7758
                     /locus_tag="VFR57_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0260000.1"
                     /translation="MTREVRVANPTQTIRDVAKIMAEIDAGAVPVGENDRLVGMITDR
                     DIAIRAVAEGKGPDTPVRDIMSKDIKYCYDDEDLEHVAENMGDLQVRRLPVVNRDKRL
                     VGIVALGDVARHEDHHTTGETTAEVSKPGGKHRQK"
     gene            7853..8149
                     /locus_tag="VFR57_11365"
     CDS             7853..8149
                     /locus_tag="VFR57_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260001.1"
                     /translation="MRAWGQPQRSGKLADGSEVHTWLSQGAPVYHSGASVGIGTGVHR
                     GGGSVGVGVGFPIGGGSVSPPAACERTLTFRDGRLVEQSWIGPDEICAEYGRAR"
BASE COUNT         1472 a         2657 c         2780 g         1361 t
ORIGIN      
        1 aaggccggcg accagcccga agccaaaccg gtcttcgacc tgaacactcg cgacctgatc
       61 gccacggcgc gtcgcatgcg cgacgagggc aggctgcctt cgggcacgga gatcgagcgc
      121 gcgccggaac tcttcatcgg cgcggccgac gtgccgacgg atccgaaacc gggctggatt
      181 ccggccgcgc tcctggcgaa ggcgcaagcc ggggcggact tcgtgcagac gcagttctgc
      241 atggacatcg gcatggtgcg gcgctacgcc gcgcggctgc gcgaggccgg gctcaacctg
      301 cccgtgctga tcggcatcgc gccgattccg tcggcgcgct cagcgcgctg gatgcgcgag
      361 aagctcttcg gcactatcgt gcccgacgag atcgtcaagc gcctagagca cgcggcagat
      421 ccgaagcgcg aggggcgcag gatctgcgtc gagctccttc agcagctcgc cgagatcccc
      481 ggcgtggccg gcgcgcacgt catggcgccg atgaacttcg ccgaaatccc ggcggcgatc
      541 gaagagtccg gggtgctgaa gcgaaagcgc tagttcgagg gcttgcggcg ctgcgccgcc
      601 tcgtatgcgg tttccgacgg ctgcgaaatg ctcaccgggt cgctcgccgg aaaagtttcc
      661 tgcacgccgg cgtcaagctt ctcctcgacg gtcgggttgg cgggcaagtc gatgtcatcc
      721 accgcgtcgc ttgcgttcgt ggtcgagccg gccgcgcgcg agcgtacgta gctcgaataa
      781 aggaagtacc ccgccgctgc ggcgaggctt agcttgatca ggggaagggc cacgtcatgt
      841 ctcctttacg aggcatcctt gcaaacttcg tgccgtcgcg gcggtaatac gccaagccga
      901 ctgcgatcgg gtcgctggcc ggaaaagttt cgagaagtcc ctggtcgagc gcgtcgtccg
      961 cggtggcgtc cggaccaagc ggctcgatat tgtcgagcgg gcggcgttcc atgggacacc
     1021 cggaacgcac ggagcatgcc cggcccgtgt gaagagctag gattgcgaaa tgcagcttgg
     1081 ctttatcggt cttggccaaa tgggtcgtcc catggtcgag cgcctaaaaa gcgcggggca
     1141 cagcctcaag gtctacaacc gcacccgctc ctcacctgac atcgcggcga aagcagatga
     1201 tgccgtcgcg ggcgaagtcg tgatcaccat gctggccgac gacgcagcgg tgcgggccgt
     1261 gtggctcgac accgggctcg cgcgccggct gccgaaaggc accatccatc tcaacatggc
     1321 gaccgtgagc atggcgatct cccgggagct cgcttccatc cagaaagaaa actacgtcgc
     1381 ggctccggtg ttcgggcgcc cgccgcttgc ggcgcagggt cagctcgaca tcatcgccgc
     1441 cggcccgaaa gcggcgctcg agtactgcca gccgcttttt tcggttttgg gcaaacaaac
     1501 cttcgtggtc ggcgacgatc ctgcgaaggc aaatgccgtc aagatcgcgc gcaacttcct
     1561 gctcgcgtgc ctgatcgaga gcctcggcga agcgttcgcg ctggtgcgca ggtgcggcgt
     1621 ggcgccggcg gacttcctga acgtgatcgg gaacacctcg ctcggctcgc cggcgtacag
     1681 gaactacggg aagatgatcg tcgagcaggc gtggctgccg gcgcagttcg ccatgccgct
     1741 cggcgtgaag gacgtcgagc tcgcgctcac caccgcgcgc gaagccggca tgcgccttcg
     1801 gctcggtgaa atgatcagag accatctgtt ggaagcgatc gcggccgggc gcgccgagga
     1861 ggactgggcg gcgctcgccg gccacatcgc gaacgaggcc ggtttgtcag aaaggtgaat
     1921 cccgcacacc gccggcatgc ggcgattcgc tgaagaactc gacgccgtgg cctaccctga
     1981 gagcgtacta gggaggcccc gatggatcac accgccaagc gagcgttgct cgagcaagcc
     2041 gtcagactac ttgggagaat gaaggtggcg aagcggctgg gcgtgcccga gccggttctc
     2101 ggggcctgga tgcgcggcga gacgccgatg cccaccatca accttctgat cctcgccacc
     2161 atgctggacg agcaggcgaa ggcgcaggac ggcgcggtca ggccctcgtg acgagcgaaa
     2221 agctcgataa gaagcgccgg cgctgatcgc gccagtctgc gccagaagcg gctccacgca
     2281 agcgctcgaa ccagcgccgc gcgtgccgac attcttttcg ttccgacggt atatttggcc
     2341 gcacatgaag ctgcccaaag atcgcgtcgc gtactcggcg atcgtcgatc gtccacccct
     2401 caagctgccg aacgatgcgc gcgtcgccgt gtggaccatc gtcaatgtcg aggagtggtc
     2461 gatcgagcgc gccatgccgc gcatggtgct ttccccgccg caaggccagc cgctgctgcc
     2521 cgatctgccc aactgggcct ggcacgagta tgggatgcga gtgggcttct ggcgcttcat
     2581 agaggtgctg gagaagttcg gcctcaaggc gacactcgcc atcaacggct cggtgatcaa
     2641 gagctatccg cgcgtggccg aggccgcgct cgaggcgggc tgggaattca tggggcacgg
     2701 ctatgtccag cggccgatgc atcacgtcga ggatcagcgc gacgcgattc ggaaaaccgt
     2761 ggccgagatc aaggcgttta cggggaagaa gccgcgcggg tgggagagtc ccggactcac
     2821 ggaaacctac gacaccatcg attggctcgc cgaggcgggc atcgaatatg tcgccgactg
     2881 ggtgctcgac gatcagccgg tgaagatcca gaccgcgcac gggccgatcg tgtcggtgcc
     2941 gtacaccgtc gagatgaacg acatctcgat gatggtgctg cagcaccatc cttctgagga
     3001 atggctgcgc cgcggaactg atcacttcga ccggctgtat gccgacggcg agaagacggc
     3061 gcgcgtgatg gcgatcagcg tgcatccgta catcagcggc gtgccccacc gcatcgccta
     3121 tctcgagcgg ctttacgaat acatccgcca gcggccgggc gtggcgctgt ggaccggaga
     3181 agagatcctt gactggtacc tgggaaaaag ctgaagcgct gctgccggtc ctgaaggagc
     3241 gcgcggcgaa atgcgaagag ctgcgccggc tgcccgacga gacgctgcgc gatttccacg
     3301 acgcaagact ctttcgcatc catcagccga agcgcatcgg cggcgaggaa ctcgaattcg
     3361 ccgcggtggt cacgtacgga gcgttgttcg cgcgcgcatg cgcgtcgaca gcgtggaact
     3421 gggtgaactt cgctgcgcac cacatgatgc tcggcatgtt tccgccgaag gcgcaggacg
     3481 agatctgggg cgcttcggtc gatgcgctca tcgcatcgtc tttcgtgttt ccggccggca
     3541 aggcgcgcaa ggtggagggc ggctatgtgc tctccggccg ctggccgttc tcgagcggcg
     3601 tcgatccgag cgagtggaac atgctcgccg ggctcgcgcg gctcgacgac catctgccgc
     3661 cggagcagcg cgtgtttctc ctcaagcgat cgcagtacaa gatcatcgat acctggcacg
     3721 cgggcggatt gcgcggcacc gggagcaacg atgtcgaggt gaaggaacag ttcgtgccgg
     3781 agcacatgac gctcgcggtc gcggacacga agggcgggcc gacgccgggc agcgcggtaa
     3841 atccgggcgt cctctataag cttccggtgt tcgcgctttt tccttacatg ctgtcgggcg
     3901 tcgcgctcgg catcgccgag ggcctcatcg aaggcttcaa tccgggcgcc gggcgcatga
     3961 ctggagcaag agtggccgag atccagagca cgcagatccg cctcgccgag gccacggctt
     4021 acgcgcgcgc atcacggctc gtgcagctcg cgaactgccg cgacgcggaa gcccagcttc
     4081 cggacatcaa gaccaaagcg cgttatcggc tcgaaggcgc gtacgcggtg gattgggccg
     4141 tgcgcgccgt ggacgtgatg ttcggtctct acggcgcgag tggcctctac gaatcgagcc
     4201 acgtaccgcg cgccttccgc gacgcgcacg ccgtcaagca gcacttctcc ttcaacaccg
     4261 acatcgccgg taccacctac ggccgcgtcg cgctcggcct ggccagcgac aacccaacgc
     4321 tgtagcacgc accccacatc atgtggtcgg cgaactgcgg ccagaaatag agcgcagcga
     4381 cttccggaaa gagggctggc gcaatgactc ggagatcaag gacggcgatc gccgcggacg
     4441 caaccatcac ccacaatacg gtgcgtcgat aaggcagcag gagccacagc accaacgccc
     4501 agaaaagcga cacggagaca tggacgagcg ctgccgcggc aagcgagttg ggcatcgcga
     4561 tcattgcccc tgccgcgcgg gtcgcttcta acggatcgcg accggtgagg agcgcgtaga
     4621 gcgtggacgg gatgccgctg aacagcgtag ccagcagcca ggcgtaaaga agggcccgct
     4681 tggtgcgcat cgggtacggc aattgcgttg gacaatcgtg ctgcccacgg tttcgggcgc
     4741 aagacaggac gcgccaacga tgaagacctt cctattgatc gtcagcctct tgctggctcc
     4801 gctgctcgcg agcgcagcca gcccgttcac gtcgctgcat ccggaggagg caattccaga
     4861 cccgaccgag cagccgtcgc ctggcccgta ttctgagttc atcggccggg tacagcaaag
     4921 gttgctcgtg ctcggcttcg gcgcaggacc ggtcaacggc gatttcggcg ccaagacgca
     4981 ggctgcgctc gctcagtttc agctctcgag ggcgattccg gcgggcggcc aactcgacga
     5041 tcagacgctt gccgaactcg gcgtggtgcg cgaagaggcg gccgccggcg caagcgcgga
     5101 acagtccgag cgctagccgc ccatgcagtg ccggggacgg cctcatgacc gtcccggcgc
     5161 gaaacggcta tttacttctt gctcggggcc ttgggatcgc tgctcgccgt gccgccggca
     5221 cccgccttct tctcggcgtg ctccggcgca gccgcatgct cctgcggcga cagtttgccg
     5281 tcattgttct tgtcgagcgt ggcgaagtcc ttgtcgtgcg gcgtgccctt cgcttcttca
     5341 ggcgtcagca tgccgtcctt gttcttgtcc agcgagttga acatcgcatc ggcgcccttg
     5401 ttgccggtgt tgctcgcgcc ctggctcgcg ctttggcttt ggctcgtgct cggagaggcg
     5461 ctcgcgtctt tggccttgtc gccggcggcc tggaccggaa ctgcgaatgc tgaagcgact
     5521 gcgattgcga tcaatgtctt gagtgtcatg tcctgtgtcc tcgggttaaa gggattgcat
     5581 cttgccgcga gcaattgaca ggccgggacg acgggaaaat cccgacccta gtccgccatg
     5641 actatggact tccaaggagg ttgtcatgca atccaagctg cttttgcaga gcgccgagcg
     5701 cgtcttcgca gtcatcttcg ggactggcga tgagcccgtc gcagggctca ccgcctttgc
     5761 ggcccagcac aagctgcgcg cgtcatcttt cacagcgatc ggcgccttca gcgaagcgac
     5821 gctcggatac ttcgactggg agaagaaaga ctacgagcgc atcgcagtgg gcgagcaggt
     5881 cgaggtgttg gcgcttgtcg gcgacatcgc actgcaggat ggcaaaccga aattgcacgc
     5941 gcacgtcgtg ctcggcggac gcgacggcaa cgctcgcggc ggccatcttc tctccgcgcg
     6001 tgtccgcccc acgctggaag tgattgtcac ggaatcgccc tcgtacctga cgcgccgcca
     6061 cgatccgcaa agcggcctcg cgctcatcga cattcgcgcg tgatccggag gcacggcgct
     6121 tgctcctcct ttcggagtag tccacgaccg gtatcccagg aaaggagatc tcgaaatgaa
     6181 gttgaatgca atcgcagttg cggtcgccgc ggcattgctg tcgttcggcg ccgtcgcaca
     6241 agagaagaag caggagtcgc aggcgggaca atcttcgccg tcgatgagcc agtcgcaagg
     6301 cgcgtcgggc tcgagcagca cccagtcgcc gtcggcggcg ggcggggcca gctccagctc
     6361 gagcagcgac aagagcgcct ccggtagcgc gggcgcatcg agcgacaagt cttcgcaagg
     6421 cggctcggcc gccgcgggtg cgtcgtcggg cggtcaggcg aagagcgagt cgaagggcgg
     6481 ttccgccgcg gccggcagcg gccagcagca gcatgacgcc gagacgatca agcaggttca
     6541 gcagaagctg aacgtcacgg ctgacggcat catgggaccg cagacgcaga aagcgctgca
     6601 ggactttcag cagcagaagg gcctggacgc gaacggtcag ctcgaccaga agacgctctc
     6661 cgccctcggc gtgagcggct cggcggcagg cggcgcgtcc ggcaagtctt cgtcctcggc
     6721 ctcctcgagc tcttccggct cctccatggg aagcggcggc tcgtccgcga gcggctctac
     6781 caccgagcag aagccccagc aggctccggc gcccacctcg ccgagcacca gcgagaagaa
     6841 gcagtagccg cgtcaagaag taccggaaag cggagggtcg cgaggctctc cgcttttctt
     6901 tttggcatac tgattgctcg agcgcgcaga tgaggcccgc cagccatagc gaagtggaga
     6961 ctctcctcaa aaaggaggcg cgctcgcggc tgccgctcct ccgccagatc ttcatttacc
     7021 tggatccgtt cgcgctcttc aaggacgcct cgcgcggcac ggcgcaagcg cgcgcgctgg
     7081 cggtttccta taaccgtgcc atgcgctgga tgctcgtgcc gtacatccgc cgttggctgg
     7141 ccatcgcggc ctcgctcggc ctgatgatcg cgccagccga agccgccgcc gcgcagcagg
     7201 cggcctttgc gatccccatg gcggcgatcg cggtggcatg ctgcatcgcc atcacgatcg
     7261 ctctggtaac ggtcgcggtc tatctcttgc tgggtaacaa ggtggtttaa cccagaggag
     7321 atctccatgc gagtcagcga agctatgacg cgcgaagttc gcgtcgccaa tcccacccag
     7381 acgatccgcg acgtggccaa gatcatggcc gagatcgacg ccggcgcggt gccggtcggc
     7441 gaaaatgacc gcctcgtcgg catgatcacc gaccgcgaca tcgccatccg cgccgtggcc
     7501 gaaggcaagg ggcccgacac gccggtgcgc gacatcatga gcaaggacat caagtattgc
     7561 tacgacgacg aggacctcga gcacgtcgcg gagaacatgg gcgacctcca ggtgcgccgt
     7621 ctgccggtcg ttaaccgtga caagcgcctc gtcggcatcg tcgcgctcgg cgacgtcgcc
     7681 cggcacgagg atcatcacac gaccggcgag accacggccg aggtgtcaaa gcccggaggc
     7741 aagcatcgtc agaagtaagt tcctcatcgc gctcgccgcg gcgctcgtcg cggcgtgcgc
     7801 gaccaccgag aaagcgcgcg aaagctggaa cggggcgagc tacgacgacg tcgtgcgggc
     7861 gtggggccag ccgcagcgca gcggcaagct cgccgacggc tcggaagtcc atacgtggct
     7921 ctcgcaaggc gctcccgtct accacagtgg ggctagcgtc ggcatcggca ccggtgtgca
     7981 ccggggcggc ggcagcgtcg gtgttggcgt cgggtttccg attggcggcg ggtccgtttc
     8041 gccgccggcg gcctgcgagc ggactctgac gttccgcgac gggcgccttg tcgagcagag
     8101 ctggatcggc cccgatgaga tctgcgcgga atacgggcgc gcccggtagg accgcagtct
     8161 tcatgctgtg cgtggtcgcg ggatgcgcca gcgaccgcga actcaacgcg gcacgcgatt
     8221 cctggcgcgg cgcgagctac gaccaggtgg ttgccgcgtg ggggccgccc
//
LOCUS       DASTPD010000335         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_181625, whole genome shotgun sequence.
ACCESSION   DASTPD010000335 DASTPD010000000
VERSION     DASTPD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181625"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFR57_11370"
     CDS             complement(<1..558)
                     /locus_tag="VFR57_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260002.1"
                     /translation="MKRRKLLQMLAAGAAFYGPWKVHHAWAQAAQKKPLVIGLTMDAS
                     GQYGASGMDERLGAMLAIREFNEKGGVLGRRIEALHMDTETTPATGSRVAERLITRNE
                     AAFLIGAVHSGVANAISQVAQKYGTVFFNTNSSSPTESGRDCHRVKFVWDGNGTNFNH
                     AIVKNAMRVSGKNWLLLTNDYVWGHN"
     gene            complement(570..1562)
                     /locus_tag="VFR57_11375"
     CDS             complement(570..1562)
                     /locus_tag="VFR57_11375"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0260003.1"
                     /translation="MVCRQAERSGSDLARGEELREPGRRMAKSEASMQRIGFIGLGLM
                     GHGMAKNLLKKGHALSFLVHRNRSNLGDLLDLKAEEASSPKELIASSDVVILCVTGTP
                     QVEANVYGANGLLEGCRKGQIVVDTSTAEPASTDRIRADFASKGVRFVDAPLARTPQH
                     AEDGKLNCMVGADPQTLAEVKPVLEKFCENIFHVGGPGSGHKVKLVYNFVAMAHAAIC
                     CESLAACARVGISLESFFQLVSAGGANSNMFQMLVPKALKGDLTGLMFTLGNAKKDID
                     YYQRMVAGASLAGPMGAALHQALVQANALGLGEKFVPSLIEAYERVNGLTVVKR"
     gene            complement(1445..>2190)
                     /locus_tag="VFR57_11380"
     CDS             complement(1445..>2190)
                     /locus_tag="VFR57_11380"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEU0260004.1"
                     /translation="KQLEELGPAERIRAFKRLIASALSSISPKSNVGAIVDERYGEEA
                     LFALTGKGMWLARPVEVPGSRPLEFEAGANIAAHLRAWPAEHVVKCLSLSPLEDLPRL
                     KALDDACIATGHELLIELVMQNPPLAEIYAAGIKPDWWKLASPQDDEAWAAIEQVIQR
                     EDPYCRGVLLLGMEASEDELERGFAAAARHPVCKGFAVGRSIFMEAARLWFADKLSDQ
                     EVISRVAKNYASLVAAWRKARQACKESVS"
BASE COUNT          348 a          731 c          693 g          418 t
ORIGIN      
        1 gttgtgcccc cacacgtagt cgttggtgag cagcagccag ttcttgccgc ttacccgcat
       61 cgcgttcttc acgatcgcgt ggttgaagtt ggtgccgttg ccgtcccaca cgaacttgac
      121 gcggtggcag tcacggcccg attcggtcgg cgagctcgag ttggtgttga agaacacggt
      181 gccgtacttc tgcgccacct gtgagatcgc gttcgccacg cccgagtgca ccgcgccgat
      241 caggaacgcg gcctcgttgc gggtgatcag gcgctcggcg acgcgcgagc cggtggccgg
      301 cgtggtctcg gtgtccatgt gcagcgcctc gatgcgccgc ccaagcacgc cgcccttctc
      361 gttgaactcg cggatcgcga gcattgcgcc gaggcgctcg tccatgccgc tcgcgccgta
      421 ttgcccgctc gcgtccatcg tgaggccgat caccagcggc ttcttctgcg cggcctgtgc
      481 ccacgcgtgg tgcaccttcc acgggccgta gaacgccgcg cccgcggcca gcatctgcaa
      541 cagtttcctc cgtttcatcg cttcctcctt caccgtttga cgactgtcag tccgttgacc
      601 ctctcgtagg cctcgatcaa agacgggacg aacttctcgc cgaggccgag cgcgttcgcc
      661 tgcaccagcg cctggtggag cgccgctccc atgggaccgg cgagcgaagc tcccgccacc
      721 atgcgctggt agtaatcgat gtccttcttc gcattgccga gcgtgaacat gaggccggtg
      781 aggtcgccct tgagcgcctt gggcaccagc atctggaaca tgttcgagtt cgcgccgccg
      841 gccgagacca gctggaagaa gctttcgagt gaaataccga ccctggcgca cgcggcgagc
      901 gactcgcagc aaatggcggc gtgcgccatg gcgacgaagt tgtagacgag cttgaccttg
      961 tgcccgctgc cggggccgcc gacgtggaaa atgttctcgc agaacttttc cagcaccggc
     1021 ttcacctcgg caagcgtctg cggatcggcg ccgaccatgc aattgagctt tccgtcttcg
     1081 gcgtgctgcg gcgtgcgcgc gagcggcgcg tcgacgaagc gcacgccctt gcttgcgaag
     1141 tcggcgcgga tgcggtcggt ggaggcaggc tcggcggtcg acgtgtcgac cacgatctgg
     1201 cccttgcggc agccctcgag aagcccgttg gctccgtaga cgttcgcttc gacctgcggc
     1261 gtgccggtga cgcacaggat gaccacatcg gaactcgcaa tgagctcttt cgggctggaa
     1321 gcttcctcgg ctttcaaatc gagcaagtcg ccgaggttgg aacgattgcg gtgcaccagg
     1381 aacgagagcg catggccctt cttcaggagg ttcttggcca tgccgtggcc catcaggccg
     1441 agtcctatga aaccgattct ttgcatgctt gcctcgcttt tcgccatgcg gcgaccaggc
     1501 tcgcgtagtt cttcgccacg cgcgagatca cttcctgatc gctcagcttg tctgcaaacc
     1561 agagccgcgc cgcttccatg aagatcgagc gccccaccgc gaagcccttg cacaccgggt
     1621 ggcgcgcggc cgcggcgaag ccgcgctcga gctcgtcctc gctcgcctcc atgccgagga
     1681 gcagcacccc ccggcaataa ggatcttctc tctgaatgac ctgctcgatc gccgcccacg
     1741 cctcatcgtc ttgcggcgaa gcaagcttcc accagtcggg cttgatgccg gcggcgtaga
     1801 tttccgcgag cggcgggttc tgcatcacga gctcgatcag gagctcgtgc ccggtggcga
     1861 tgcaggcgtc atcaagcgct ttcaaccgag gcagatcttc cagcggggac aacgaaaggc
     1921 acttgacgac gtgctcggcg ggccaggcgc gcaagtgcgc cgcgatgttt gcgcccgcct
     1981 cgaactcgag cggccggctg cccggcactt cgaccgggcg cgcgagccac atccccttgc
     2041 cggtgagcgc gaagagcgcc tcttctccgt aacgttcgtc gacgatggcg ccgacattgc
     2101 ttttcgggga aatggacgag agtgcggaag caatgaggcg cttgaacgcc cggatcctct
     2161 cggcggggcc gagttcctcg agctgcttgc
//
LOCUS       DASTPD010000336         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1613462, whole genome shotgun sequence.
ACCESSION   DASTPD010000336 DASTPD010000000
VERSION     DASTPD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613462"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VFR57_11385"
     CDS             <1..1357
                     /locus_tag="VFR57_11385"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEU0260005.1"
                     /translation="GMKARNADAVIVAGGGIGGLAAALALARKGFRSVVLEQASQFGE
                     IGAGIQIAPNAWHALDALGVGGLVKREAVFIEHLLMFDGVSGEKVVDIPLDKRFAKRF
                     GNPYAVTHRADIHGALLDGCKALPNLIELRTSTRVVGFEDDGRGVTVRTDKEPIRGTA
                     LVGADGGRSVIRESIVGDKLPLITGHMCYRAVLNIEDVPKDLRFAAATLWAAHNAHIV
                     HYPLRGWKLFNLVATIIGRHTSGGHNELAQPDEVLPFFAHYCEKPLKLMRTPKEFRRW
                     MLLHREPVNNWSRGRVTLLGDAAHFMLQYMAQGAAMAMEDAVCLGLCADETDGDFAAA
                     FKNYQEKRLVRATRVQISANRLVGMIFHVPDGLERLVRNDMYRGRPVERFYDALEWVF
                     SAPDYVRNFNERGGAARSRRSSAPRTASSRAAARRRGSPARAALPARRPPSRRTASRG
                     R"
     gene            complement(1272..2345)
                     /locus_tag="VFR57_11390"
                     /pseudo
     CDS             complement(1272..2345)
                     /locus_tag="VFR57_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
     gene            complement(2329..2622)
                     /locus_tag="VFR57_11395"
     CDS             complement(2329..2622)
                     /locus_tag="VFR57_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260006.1"
                     /translation="MQRLCRLLCLVSLAMASSAHAGGVVVVSSGSASGIWLVLGAWTA
                     MLPRPVPEVSPHHIRADQAAARARDSCIWVAGVRHCPLPEPRAPLHPPRAEPR"
     gene            2683..3618
                     /locus_tag="VFR57_11400"
     CDS             2683..3618
                     /locus_tag="VFR57_11400"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0260007.1"
                     /translation="MKPGNKVKRIEGVAAPVITPFRKDYSPDAERFVRHCRWLLKSGC
                     AGLAVFGTNSEANSMSVGEKRKLLEALVAGGIPATTLMPGTGHCALSDSIEMTRAAVE
                     LGCAGVLMLPPFYYKGVSDEGLYRNFAEVIERVGDERLSLYLYHIPPVAQVPITLSLI
                     ERLLSKYPGIVAGVKDSSGDWSNTKAMLDSFAKSGFDVFAGSEVFLLDNMRNGGKGCI
                     TATGNINPGPIANVHKNWRSADADRLQAGITATRKIVQKQPMIPALKSAVAHFGNDVE
                     WRTCRPPLVELSSAQEEELIRDLKAAGFTMPGLAG"
     gene            3628..4290
                     /locus_tag="VFR57_11405"
     CDS             3628..4290
                     /locus_tag="VFR57_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0260008.1"
                     /translation="MKTALVTGAARGIGRAVADALQKAGLRVLRLDLQGEIAYDLTDL
                     AGIPKLIDGLGEIHVLVNNAGVQTAVSIDQYTEQQRARILRVNLEAPVELIRAVSRQM
                     LARKNGRIVNLASVAAYTPHTDLWYGVTKAGVVSFTRSFAAYLGPHGIQVNAVAPGPI
                     DTPLLDKAQPERVEELMKRVYTRRKGRPEEVAEAIRWLALDAPEIINGAVIDVTDGSF
                     LR"
     gene            complement(4265..5095)
                     /locus_tag="VFR57_11410"
     CDS             complement(4265..5095)
                     /locus_tag="VFR57_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU0260009.1"
                     /translation="MHRRGHQRDAAHHHRPAARPAEQDLIVHLVDGTYELFRHFYGLR
                     RFHKQKDPPLGAVHGVLGTIRQMIEKGATHIGVATDHVIESFRNDLWPGYKTSEGVEP
                     ALLAQFHPLEEELSAMGVAVWPMVELEADDALASAARTAWADPRVEKVCIWTPDKDLA
                     QCVQGNRVVQVHRRTNTILDAAAVRNKFGVGPERIPDFLALVGDSADGYPGIDGIGPV
                     GAARLINQYGPIEDFPPAVLGERRDLALLFKNLATLRTDARLFDDVDELIPSAGRSRP
                     "
     gene            complement(5020..>5923)
                     /locus_tag="VFR57_11415"
     CDS             complement(5020..>5923)
                     /locus_tag="VFR57_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0260010.1"
                     /translation="TGIFNSHLMMALLVEKYGSEALKKKYLPRFASGEIRGGLALTEP
                     NAGTDLQAIRLRARREGDHYVLNGTKTWISNGIQGSCFAVLAKTAPDAEPRHKGMAMF
                     LCEKGPGFSASRKLEKLGYKGIDSAELVFQDYRVPAANLIGEEGRGFHQAVGGLELGR
                     LNVAARGVGVAKAALDEAVRYSQQRQTFGKPICEHQAIQLKLGEMATRVEAARLLVKN
                     AAEIYDRGERCDMEAGMAKYFATEAAFENAHEAMRIHGGYGYSKEFAVERLYRDAPLL
                     CIGEGTNEMQRIIIARQLVQRNKI"
BASE COUNT         1032 a         1986 c         1923 g          982 t
ORIGIN      
        1 tggaatgaaa gcgcggaacg cagacgccgt catcgtcgcc ggcggcggca tcggcgggct
       61 cgcggcggcg ctcgccctcg cgcgcaaagg cttccgctcg gtggtgctgg agcaggcctc
      121 ccaattcggc gagatcggcg ccggcatcca gatcgcgccc aacgcctggc acgcgctcga
      181 cgcgctcggc gtcggcggcc tggtgaagag ggaagcggtc ttcatcgagc atctcctcat
      241 gttcgacggg gtgagcggtg agaaggtggt cgacatccct ctcgacaagc gcttcgcgaa
      301 gcgcttcggc aacccctacg cggtgaccca ccgcgccgac atccacggcg ccctcctcga
      361 cggctgcaag gcgctgccga acttgatcga gctgcggacc agcacgcgcg ttgtggggtt
      421 tgaggatgac ggaagaggag tcactgtaag aacggacaag gagccaattc gcggtaccgc
      481 cctagtgggt gcggacggcg ggagatcggt gatccgcgaa agcatcgtcg gcgacaagct
      541 gccgctcatc acggggcaca tgtgctaccg cgcggtgctc aacatcgagg acgtgccaaa
      601 agatttgcgc ttcgccgccg cgacactctg ggccgcgcat aacgcgcaca tcgtccacta
      661 cccgctgcgc ggctggaagc tcttcaacct cgtcgccact atcatcggaa gacacacgag
      721 cggcgggcac aacgagcttg cgcaacccga cgaggtgctc cccttcttcg cgcactactg
      781 cgaaaagccg ctcaagctca tgcgcacgcc gaaagagttc cgccgctgga tgctgctgca
      841 ccgtgagccg gtgaacaatt ggagccgtgg gcgcgtgact ctcctcggcg acgcggcgca
      901 cttcatgctg cagtacatgg cgcaaggcgc cgcgatggcg atggaggacg cggtgtgcct
      961 cgggctctgc gccgacgaga ccgacggaga ttttgctgcc gcattcaaga actatcagga
     1021 aaagcgcctg gtccgtgcca cccgcgtgca gatctcagca aaccgcctcg tcggcatgat
     1081 ttttcatgtg cccgacgggc tcgagcgcct ggtgcgcaac gacatgtacc gtggccggcc
     1141 ggtcgagcgg ttctacgacg cgctcgagtg ggtgttcagc gctcccgact acgtgcgtaa
     1201 tttcaacgaa agaggaggtg cggcccgatc tcggcgatcg agcgcacccc gcacagcttc
     1261 atcgcgcgct gcagctcgtc ggagaggatc tccagcgcgc gccgcgctcc cggctcgccg
     1321 cccgccatca cgccgaacag cgtcgcgcgg ccggtaagca cggcctgcgc gccgagcgcg
     1381 cgcgccttga cgatgtccac gccgcggcgc acgccgccgt cgagcagcac cgtcgttctg
     1441 ctccccacag ctgaagcaat cccgggcaat gcgtccaagg tcggcgtagc gccgtcgagc
     1501 tgccggccgc cgtgatttga aacgatcacg ccgtcgcagc cggccgccac cgcgcgctcg
     1561 gcgtcgtcgg gacgctcgac gcccttgaga agcagcttcc ccggccagag ctcgcgcagg
     1621 cgcttaagct cctcccagtc gaacgacgcg tccatctcgc gcccgacctg gctcgcgatg
     1681 tccgtgacct tcacgctaga gaacttgtag ccgtggaagt tctccatccc cggcagcccg
     1741 tggcgcatga tgtcgaaaag ccacgcgggc ttgagccaga ggtcccggga gttgcgccag
     1801 tttggccgga acggcgtgat gaagccgttg cgggggtcgc gctcgcgctt gccggaggtc
     1861 gcgagatcca ccgtgacgag cagcgcttcg aagctcgaat tcttcgcccg ttcgatcaat
     1921 ctttccctga actggcggtc gcgcaccaca tagagctgaa accacaaccg cccgcccacc
     1981 tcggcgatcg tctcgatcga cgcggtcgcc ggcgtggcga gcgtgtacgg aatgccgaag
     2041 gccttcgcga tgcgcgcaag catcacgtca gcgcccggcc gccccgcgcc gatgccgccg
     2101 gtggcgctga tggctagggg caggtttgtg ggtttatcaa gcagattcac tcgcgtatcg
     2161 acttccgcga cgttcaccag cacccggggc agcagcttca cccgctcgaa cgccgcgcgg
     2221 ttctcgcgca gcgtgacttc gtcttccgcg ccgccgtcga aaaagtcgaa gatcgcgcgt
     2281 ggcaggcgac gcttcgccag cacccgcagg tcctcgatgt tgacggctct agcgaggttc
     2341 ggcacgcggg ggatgcaggg gcgcgcgagg ctccggcagc gggcaatggc gcacgcccgc
     2401 gacccagatg cacgaatccc tggcgcgtgc tgcggcctgg tcggcgcgga tatggtgcgg
     2461 cgagacttcc gggaccgggc gcggcagcat agcggtccag gcgccgagga cgagccagat
     2521 cccggatgcg ctccccgagc tcacgaccac cacgcccccg gcatgggcgc tgctcgccat
     2581 ggcgagcgac acaaggcaaa ggaggcggca aagtcgctgc ataattggcg gctcactcta
     2641 gcggaatgcc cagaagctcg gcaataaatt caacggagca ccatgaaacc aggcaacaag
     2701 gtgaagcgaa tcgagggcgt ggctgcgccc gtgatcaccc ccttcaggaa ggattactcc
     2761 cccgacgccg agcgcttcgt acgccactgc cgctggctcc tcaagtccgg ctgcgcggga
     2821 ctcgcggtgt tcggcaccaa cagcgaagcg aattcgatgt cggtcggcga gaagagaaag
     2881 ctgctcgagg ccctggtcgc cggcgggatc ccggcaacga cgctcatgcc cgggacgggg
     2941 cattgcgcgc tttcggactc gatcgaaatg acgcgcgccg cggtcgagct cggctgcgcg
     3001 ggcgtcctca tgctcccgcc cttctactac aagggcgtgt ccgacgaggg gctgtatcgc
     3061 aacttcgccg aggtgatcga gcgcgtcggc gacgagcggc tatcgctcta tctgtaccac
     3121 atcccgccgg tagcacaggt tcccattact cttagtctta ttgaaagatt gctttcgaaa
     3181 tacccgggga tcgtagcggg cgtaaaggac tcttccggcg actggtcgaa caccaaggcg
     3241 atgctcgata gcttcgcgaa gagcggcttc gacgtgttcg caggcagcga ggttttcctg
     3301 ctcgacaaca tgcgtaacgg cggcaagggc tgcatcaccg ccacgggcaa catcaacccg
     3361 ggcccaatcg ccaacgtcca caagaactgg cgctcggccg acgccgacag gcttcaagcc
     3421 ggcatcacgg ccacgcgcaa aatcgtgcag aagcagccga tgattccggc gctcaagagc
     3481 gcggtggcgc acttcggcaa cgatgtcgaa tggcggactt gccgtccccc gctcgttgag
     3541 ctgagctccg cccaggaaga agagctcatc cgagacctga aggccgccgg cttcacgatg
     3601 cccggtctgg ccggctgaga tgcggggttg aaaacggcgc tcgtgacggg cgcggcgcgc
     3661 ggcatcggcc gcgccgtcgc cgatgcgctt cagaaagccg gattgagggt cttgcggctc
     3721 gatctgcagg gcgagatcgc ctacgacctg accgaccttg ccggcattcc aaaactgata
     3781 gacggcttgg gggaaattca tgtcctggtg aacaacgccg gcgtgcagac ggcggtctcc
     3841 atagatcagt acacggaaca gcagagagcc aggatactgc gcgtcaatct cgaggcgccg
     3901 gtcgaattga tccgggcggt gtcgaggcag atgcttgccc gcaagaacgg gcgcatcgtc
     3961 aacctcgcct cggtcgccgc ctacacgccg cacaccgacc tctggtacgg cgtcaccaag
     4021 gccggcgtgg tgagcttcac gcgcagcttc gcagcctacc tcggccctca cggcatccag
     4081 gtgaacgccg tcgcgcccgg cccgatcgat accccccttc tcgacaaggc gcagcccgag
     4141 cgcgtggaag agctcatgaa gcgcgtctac acgcggcgca agggcaggcc cgaagaagtc
     4201 gccgaggcga tccgctggct cgcgctcgac gcgccggaga tcatcaacgg cgcggtcatc
     4261 gacgtcacgg acggctcctt cctgcgctag gaatcagctc gtccacatcg tcgaaaagcc
     4321 gcgcatcggt ccgaagcgtg gccaggttct tgaaaagcag ggcaaggtcc ctgcgctcgc
     4381 ccagcactgc cggcggaaag tcctcgatcg ggccgtattg gttgatgagc cgggccgcgc
     4441 ccacagggcc gatgccgtca atgcccgggt agccgtcggc gctgtcaccg acgagcgcga
     4501 ggaagtcggg gattcgctcc ggcccgacgc cgaatttgtt gcgcaccgcc gccgcgtcaa
     4561 gaatggtgtt tgttcttcta tgaacctgta ctacccggtt gccctgcacg cactgcgcga
     4621 ggtccttgtc cggagtccag atgcacacct tctccacacg cgggtccgcc cacgcggtgc
     4681 gggcggccga agcgagcgcg tcgtcggctt cgagctcgac catcggccac accgcgacac
     4741 ccatcgcaga gagctcctcc tcaagcgggt ggaactgcgc gaggagcgcc ggctcgactc
     4801 cctcgctcgt cttgtacccg ggccagaggt cattgcggaa ggattcgatg acgtggtcgg
     4861 tcgcgacgcc gatgtgcgtc gcgcccttct cgatcatctg ccggatggtg ccgagcacgc
     4921 cgtgcacggc gccgagcggc ggatccttct gcttgtggaa tcgccgcagg ccgtagaagt
     4981 ggcggaaaag ctcgtacgtc ccgtcgacga gatggacgat cagatcttgt tccgctggac
     5041 gagctgccgg gcgatgatga tgcgctgcat ctcgttggtg ccctcgccga tgcagagaag
     5101 cggcgcgtcg cggtagagac gctcgaccgc gaactccttg gagtagccgt agccgccgtg
     5161 aatgcgcatc gcctcgtgcg cgttctcgaa cgccgcttcg gtggcgaaat atttcgccat
     5221 tccggcctcc atgtcgcagc gctcaccgcg gtcgtagatt tcggctgcgt tcttcaccag
     5281 cagccgcgcc gcctcgacgc gagtcgccat ctcgccgagc ttgagctgga tcgcctggtg
     5341 ttcgcagatc ggcttgccga aggtctggcg ctgctgtgag tacctcaccg cctcgtccag
     5401 cgccgccttc gcaacgccga cgccgcgcgc ggcgacgttg agacgcccaa gctcgaggcc
     5461 gccgactgcc tggtggaagc cgcggccttc ctcgccgatc aggttggccg ccggtacgcg
     5521 gtagtcctgg aacacgagct ccgccgagtc gatgcccttg tagccgagct tctccagctt
     5581 gcggctcgcg ctgaaccccg ggcccttctc gcacagaaac atggccatgc ccttgtggcg
     5641 cggctcggcg tcgggagcgg tcttggccag caccgcgaag cacgagccct ggatgccgtt
     5701 cgagatccag gtcttggtgc cgttcaggac gtaatggtcg ccttcacggc gcgcgcgcag
     5761 gcggatcgcc tgcaggtcgg tgccggcgtt cggctcggtc agcgccaggc cgccgcggat
     5821 ctcgccgctg gcgaatctgg gcaggtattt cttcttcagc gcttccgagc cgtatttctc
     5881 caccagcagc gccatcatca ggtgcgagtt gaagatgccg gta
//
LOCUS       DASTPD010000337         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_182091, whole genome shotgun sequence.
ACCESSION   DASTPD010000337 DASTPD010000000
VERSION     DASTPD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182091"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFR57_11420"
     CDS             complement(<1..509)
                     /locus_tag="VFR57_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0260011.1"
                     /translation="MARGLWKGAISFGLVHVPVELHSAKKRSTELDMTMLDKRDLAPV
                     GYQRVNKSTGKEVPWADVVKGYEYEDDKYVVLSDEDFRRANPEAAKTVDIQAFVDLID
                     IAPQYFDTPYYLVPGKRGEKAYALLRDTLKKAGKAGIAVVVIRTRQYLAALMAQDDIL
                     VLNTLRHHD"
     gene            complement(573..1088)
                     /locus_tag="VFR57_11425"
     CDS             complement(573..1088)
                     /locus_tag="VFR57_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260012.1"
                     /translation="MKVKLTAVTLGLAFGLASAAQAQTTPAPAKRDSAPPRAEQSERK
                     AKDAAEERIEQEYKAAREKCNSMEGNAKDICMAEAKGKQKVAKAELDAKDAKDQAKAQ
                     RKVQEARADATYEVAKEKCDDQKGEAKDACVKQARVERDRAKGKAEKTAEARQEKQRA
                     ATGSTAPEKQK"
     gene            complement(1116..1280)
                     /locus_tag="VFR57_11430"
     CDS             complement(1116..1280)
                     /locus_tag="VFR57_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0260013.1"
                     /translation="MLSWALAFFIIAIIAAVFGFTGIAAGAAEIAKILFVVFVVLFLV
                     SLVAGLLRRP"
     gene            1474..2424
                     /locus_tag="VFR57_11435"
     CDS             1474..2424
                     /locus_tag="VFR57_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0260014.1"
                     /translation="MQPDDSFPLMTWRARPDMACEYVNREWRKYTGYTMEQAMGQGWA
                     RCLHAEDLTRWLDTCVAAFDRREPFEIEYRLRRRDGEYRWVLERAAPRYSADELFVGF
                     GAVCFEIDRHKRESESLARALERERRLRIATEEASRLRHGLMVSVLQELDLPSRAIAT
                     WAAHLRGQMPHGVALEAIERNARIQNRIIATLLQLAQVSGVPPRDRPLLSGVRVLVVG
                     HHELVKPLEVAGADVRAAPNPDEALATLNSWRPDVVLAESNESFIRTLRSLPAERGGC
                     LRAAALTDGAAAAPAAGYDAQLATPVEPVALLATVARLAA"
     gene            2489..2686
                     /locus_tag="VFR57_11440"
     CDS             2489..2686
                     /locus_tag="VFR57_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU0260015.1"
                     /translation="MNWDRIQGNWKQMTGKLKEKWGKLTDDDFDQVEGNRDQLVGKIQ
                     ERYGIAKDEAERQVRDWESRL"
     gene            2780..3109
                     /locus_tag="VFR57_11445"
     CDS             2780..3109
                     /locus_tag="VFR57_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0260016.1"
                     /translation="MRLIRQSILNAVVAAIAAALLSACASSETKRSTGEFTDDAALTA
                     KVKSAIATDVGARTAGAINIETYRGVVQLTGFVSAPEQAQRAEAAAKKVEGVRSVKND
                     VRIKPSS"
     gene            3120..3722
                     /locus_tag="VFR57_11450"
     CDS             3120..3722
                     /locus_tag="VFR57_11450"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0260017.1"
                     /translation="MATSAAAKARHPHFSLPPLPYAEDALEPVISAETLRLHHDKHHK
                     KYVDTANELLAKDPIQAASLEEVVRASRGKLFNNAAQAWNHDFYWQSLSPKRQRPSGA
                     LRARLEKDFGNYDRFAEAFAAAALGQFGSGWAWLVDKDGRLQVMATSNAETPMAQGIK
                     CLLTVDVWEHAYYVDYRNERERYVSAVMDRLNWESAEKNL"
     gene            complement(3719..3904)
                     /locus_tag="VFR57_11455"
     CDS             complement(3719..3904)
                     /locus_tag="VFR57_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260018.1"
                     /translation="MESAYVYAEAFRAAANAVGGEQELAERLGVSAEELHVWIEARGT
                     PPLEPFLEALDLLEPSR"
BASE COUNT          678 a         1329 c         1301 g          718 t
ORIGIN      
        1 tcgtcgtggt ggcgaagcgt gttcagcacc aggatgtcgt cctgcgccat cagcgccgcc
       61 aggtactgcc tggtgcggat caccacgact gcgatcccgg ccttgccggc cttcttgagc
      121 gtgtcgcgga gcagcgcgta ggccttctcg ccgcgcttgc ccgggacgag gtagtaaggc
      181 gtatcgaaat actgcggcgc gatgtcgatc aggtcgacga acgcttggat gtcgacggtc
      241 ttggccgcct ccggattggc gcggcggaaa tcctcgtcgg agagcacgac gtacttgtcg
      301 tcctcgtact cgtagccttt caccacatcc gcccacggca cttccttgcc cgtcgactta
      361 ttgacgcgct ggtagccgac cggggcgaga tcgcgcttgt cgagcatggt catgtcgagc
      421 tcggtcgagc gcttctttgc tgaatgcagc tcgaccggca cgtggaccag cccgaagctg
      481 attgctcctt tccacaaccc gcgcgccatc ggctgctgcc ggcgtgatcc cgtggcgtgt
      541 cctcctcttg cgaggaggaa cgctccggat cgttacttct gcttctcggg cgcagtacta
      601 ccggtggcgg cgcgctgctt ctcctgccgt gcttcggcag tcttctccgc cttgcccttg
      661 gcgcggtcgc gctccacgcg cgcctgcttg acgcacgcat ccttggcttc gcccttctgg
      721 tcgtcgcact tctccttcgc gacctcgtag gtggcgtcgg ccctggcttc ctgcaccttg
      781 cgctgcgcct tggcctggtc cttcgcgtcc ttcgcgtcga gctccgcctt ggcgaccttc
      841 tgcttgccct tcgcttcggc catgcagatg tccttggcgt tgccttccat gctgttgcac
      901 ttctcgcgcg cggccttgta ctcctgctca atgcgctctt ccgcggcgtc tttcgctttg
      961 cgctcggact gctcggcgcg cggcggcgcg gaatctcgct tggccggcgc cggcgtggtt
     1021 tgcgcctgcg cggcgctcgc caggccgaag gcaaggccca gcgtgacagc ggtgagtttc
     1081 actttcatag gcgtatctct cctttggctt tgtctctaag gccttctcag caatcccgcc
     1141 accagcgaga cgaggaaaag gaccacgaac acgacgaaca ggatcttggc gatctcggcc
     1201 gctccggcag cgatgccggt gaagccgaaa accgccgcga tgattgcgat gatgaagaac
     1261 gccagcgccc aactcagcat aacggctcct cgttaagtag tttgcagcac ggcaggtgca
     1321 gcaaagtcca tgccgagcat ggcgcgcggg cgcttcgcat gtaacgttgg ggtgggggct
     1381 gtaagaacta cgtgtcgtgc ggcggcggcc ttcgtagcga aaccgctacg ggcacggctc
     1441 ctgctgtgtc gtcttcagcc ccatccgaac ggcatgcagc ccgacgactc ttttccactc
     1501 atgacctggc gcgcgcgccc ggatatggcg tgcgagtacg tcaaccgcga gtggcggaag
     1561 tacaccggtt acacgatgga gcaggccatg gggcagggtt gggcgcgctg ccttcacgct
     1621 gaggacctca cgcgctggct cgacacctgc gtggctgcgt tcgaccggcg cgagccgttc
     1681 gagatcgagt accgcctgcg ccggcgcgac ggcgagtacc gctgggtgct tgagcgcgcg
     1741 gcaccgcgtt attccgcaga cgagcttttt gtaggcttcg gtgccgtgtg cttcgagatc
     1801 gaccgccaca agcgcgagag tgaaagcctc gcgcgcgcgc tcgagcggga gcggcgcctg
     1861 cgcatcgcga ccgaggaggc cagccggctg cgccacgggc tcatggtttc ggtgctgcag
     1921 gagctcgacc tgccctcgcg agccatcgcg acctgggcgg cgcacctgcg cggccagatg
     1981 ccgcatggcg tcgcgctcga ggcgatcgag cgcaacgcac gcatccagaa ccgcatcatc
     2041 gccacgctgc tccagcttgc gcaggtgtcc ggcgtgccgc cgcgcgaccg gccgctgctc
     2101 tcaggggtgc gcgtgctcgt cgttggtcac cacgagctcg tcaagccgct cgaagtcgcc
     2161 ggcgcggatg tgcgcgccgc cccgaacccg gacgaggcgc tcgcgacgct aaattcgtgg
     2221 cggcccgacg tggtcctcgc cgagtccaac gaatcgttca tccgcacgct gcgctcgctg
     2281 cccgcggaac gcggcggctg cctgcgcgcg gcagcgctca ccgacggggc cgcagcggcg
     2341 cccgccgcgg ggtacgacgc gcagctggcg acgccggtgg agccggtggc gctgctcgcg
     2401 accgtcgcgc ggcttgcggc atagctcttg cttttcacca atggaagcac tcgaagtgac
     2461 ctttttgttt tgataggaga gaagacgaat gaactgggat cgtatccagg ggaactggaa
     2521 gcagatgacc ggaaagctca aggagaagtg gggcaagctc accgacgacg atttcgatca
     2581 ggtcgaaggc aaccgcgacc agctcgtcgg gaagatccag gaacgctacg gcattgccaa
     2641 ggatgaagcc gagcgccagg tcagagactg ggaatcgcgt ctctagtgcc aacgcaagag
     2701 aagaaggaga aaccgcaacc ggcgaagtgg ccgttcgatc ctatgcagcc cggcgaccac
     2761 gagccggtgg cggaccccca tgagactgat aagacagagc attctgaacg ccgttgtggc
     2821 ggcgatcgcc gccgcgctcc ttagcgcctg cgcttcgagc gagaccaagc gcagcaccgg
     2881 cgagttcact gacgacgccg cgctcaccgc gaaagtgaaa agcgcgatcg ccacggacgt
     2941 gggcgcgcgc actgctggcg cgatcaacat cgagacctac cgtggcgtgg tccagctcac
     3001 cggcttcgtc tccgcgcctg agcaggcgca gcgcgccgag gccgcggcga agaaggtcga
     3061 gggcgtgcgc tcggtcaaga acgacgtccg catcaaaccc agctcgtagg agccagcgca
     3121 tggcaacttc cgcggccgcg aaggccaggc atccgcattt ctccttgccg ccgcttccgt
     3181 acgcagaaga cgcgctcgag ccggtgatct cggccgagac gctcaggctt caccacgaca
     3241 agcaccataa gaagtacgtc gacaccgcga acgagcttct tgcaaaggat ccgatccagg
     3301 cggcctcgct ggaagaagtg gtgcgcgcgt caagaggcaa gcttttcaac aacgccgcgc
     3361 aggcgtggaa tcacgacttc tactggcagt cgctttcccc gaagcgccaa cgtccctcgg
     3421 gcgcgctgcg cgcgcgcctg gaaaaggatt tcgggaacta tgatcgcttc gccgaagcct
     3481 tcgcggccgc ggcgctcggg cagttcggca gcggctgggc ctggttggtc gacaaggacg
     3541 gccggctgca ggtgatggcc acatccaacg ccgagactcc gatggcgcag ggcatcaagt
     3601 gcctgcttac ggtggacgta tgggagcatg cctactacgt cgactaccgc aacgagcgcg
     3661 agcggtatgt gtccgcggtc atggaccgcc tcaattggga gtccgccgaa aagaacctct
     3721 agcgagaggg ttcgagcagg tccagggctt ccaggaaagg ctccagcggc ggagtgccgc
     3781 gagcttcgat ccagacgtgc aattcttccg cgctcacccc cagacgctcg gccagctcct
     3841 gctcgccgcc gaccgcgttg gcagcagccc ggaaagcttc ggcatagacg taagccgact
     3901 ccatgccaca accccgagca tgcggcgtgc caactttgcc tggttcttgc gggctaaccc
     3961 ggtgagcctg aattcaggcc ttggctctcg atggaacgct gcaacgcggc caaagcggca
     4021 tccacg
//
LOCUS       DASTPD010000338         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_799434, whole genome shotgun sequence.
ACCESSION   DASTPD010000338 DASTPD010000000
VERSION     DASTPD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799434"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..853
                     /locus_tag="VFR57_11460"
     CDS             104..853
                     /locus_tag="VFR57_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0260019.1"
                     /translation="MDFDLSQVAQTLAIYALPVLFAITLHEAAHGYVARHFGDMTAHA
                     QGRISLNPVRHIDVIGTIVVPLLILLVSYGKFLFGWAKPVPVNYSALRKPRQHMAWVA
                     AAGPGANLLMALFWALALRASVSMTSRADAWLEIARQYGVSGLIDAVMGHGNGAAEFL
                     IGVSAAGILVNLLLMLLNLLPILPLDGGRIVASLLPSRAAWRFAKLEPFGLPLLLLLL
                     FVPVYGSNALSLILSPLLFESEALVRAMVLL"
     gene            854..2056
                     /locus_tag="VFR57_11465"
     CDS             854..2056
                     /locus_tag="VFR57_11465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU0260020.1"
                     /translation="MYETRVLSGMRPTGSLHLGHYHGVLKNWIRLQAEYPCLFFVADW
                     HALTTDYEKPGDLQQTGIDMVIDWLAAGVDPAQAMIFVQSQVTEHAELHLLLSMMAPL
                     GWLERVPTYRDQQQKLADKDLATYGFLGYPLLQAADILVYRAKYVPVGEDQVPHLEFA
                     REVARRFNHLYGREPGFEDKAKTAVKKMGTKKARLLNELRTKFIEQGDAGALARGQAL
                     LKEQQNLSRGDRERLYGWLEGTGKKILVEPEALLTEASKMPGLDGQKMSKSYGNTISL
                     REEPEVVAKKIKTMPTDPARVKRSDPGNPEKCPVWELHKVYSDAARKDWVMRGCTTAG
                     IGCLECKQPVIDAINLELAPMRARAQPYLEDSALVKNIIADGCEKAKRLAAETMRDVR
                     EAMGLSYS"
     gene            2053..2826
                     /locus_tag="VFR57_11470"
     CDS             2053..2826
                     /locus_tag="VFR57_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU0260021.1"
                     /translation="MRAKVYGEPVIELPHDLYIPPDALEVVLEAFEGPLDLLLYLIRK
                     ENLDILDIPMAPLTRQYLEYVDMMRSTNLELAAEYLVMAAMLMEIKSRLLLPRPPAAE
                     PIEDDPRAELVRRLLEYERMKKAAQGLDELPQLGRDVIAISVWIEKQVSQRLPEVNAR
                     DLAEAWRGLLHRARLSAHHRVSREELSVRAHMSSILRALRERRVVEFGELFEPQRGIA
                     VLVVTFLALLELARELLIEITQSAGFGPIYVKLAHAQSA"
     gene            2810..3412
                     /gene="scpB"
                     /locus_tag="VFR57_11475"
     CDS             2810..3412
                     /gene="scpB"
                     /locus_tag="VFR57_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU0260022.1"
                     /translation="MLNPPEAKRILEAALLASQEPVSAIDLKRLFDDELGVDTIRSLL
                     AELADEWRGRAVELVSLSSGWRFQTRPEFQPYLERLLPERPPRYSRAVMETLAIIAYR
                     QPVTRGDIEDVRGVGVSTQIIQALETRGWIDVVGHRETPGRPALYATTTKFLDDLGLR
                     SLEELPPLEEIARTLQLEVVSTGKAEVREPEAQPATGTDG"
     gene            3417..4112
                     /locus_tag="VFR57_11480"
     CDS             3417..4112
                     /locus_tag="VFR57_11480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0260023.1"
                     /translation="MQKLLAGAGLASRREIESWISAGRVSVNGRLAKLGDRAGPADSI
                     LLDGKPVVAAARTQPRVLLYHKPVGELVTRSDPQGRPTVFSNLPPGRWISVGRLDLNS
                     SGLLLFTDSGELANRLMHPRYGLEREYSARIRRPLSAAEEKRLLQGIPLDGLLARFDR
                     IEEMKSGAGTNRWYRVVLREGRNREVRRIFEALGHPVSRLVRMRYGPIELPADLPPKS
                     WRELPNPAILQRL"
BASE COUNT          741 a         1360 c         1406 g          731 t
ORIGIN      
        1 gtgatcgacc tcaccgagaa aacgccgacc gtgttgcgcg caggcaaggg ctcgctcgcg
       61 ccctttgcgg tagagcgcgt ctgataagat cccggacgca agaatggact tcgacctcag
      121 ccaggtcgcc cagaccctgg ccatttacgc gctcccggta ctattcgcca tcacgctgca
      181 cgaggccgcc cacggctacg tggcgcggca cttcggggac atgaccgcgc acgcgcaggg
      241 gcgcatcagc ctcaacccgg tgcggcatat cgacgtcatc ggcactatcg tggttccgct
      301 gctcatcctg ctcgtctcgt acggcaagtt tcttttcggc tgggccaaac ccgtgcctgt
      361 caattactcg gctctgcgca agccgcgcca gcatatggcg tgggtggctg cggccggtcc
      421 tggcgccaat ttgctgatgg cgcttttctg ggctttggcg ctgcgcgcct cagtgagcat
      481 gacgagtcgc gctgatgcct ggctggaaat cgcacggcaa tacggcgtaa gcggcctgat
      541 cgacgcagtc atgggacacg gcaacggggc cgccgagttc ctgatcgggg tgagcgccgc
      601 tgggattctc gtgaacctgc tcctcatgct gctcaacctc cttccgatcc tgccgctcga
      661 tggcgggcgc attgtcgcga gcctgctgcc cagccgggcg gcgtggcggt tcgcgaagct
      721 cgagccgttc ggcttgccac tgctcctgct cctgctgttc gtgccggtct acgggtccaa
      781 tgcgctcagc ctgatcctga gcccgctgct tttcgagtct gaagcgttgg tgcgcgccat
      841 ggtgctgctt tagatgtacg aaacccgggt gctgtccggc atgcggccta ccggaagctt
      901 gcacctcggc cactaccatg gcgttctgaa gaactggatc cgcttgcagg cagagtaccc
      961 gtgtctcttc ttcgttgccg actggcacgc gctgaccacc gactacgaga agcctggcga
     1021 tctgcagcag accgggatcg acatggtgat cgactggctc gccgcggggg tcgacccggc
     1081 acaggcgatg atctttgtgc aatcgcaggt gaccgagcac gccgagctgc acctgttgct
     1141 ttcgatgatg gcgccgctcg ggtggctcga gcgcgtgccg acgtaccggg accagcagca
     1201 gaagctggcc gacaaggacc tcgccaccta cggctttctc ggttacccgc tgctgcaggc
     1261 ggccgacatc ctcgtctacc gggcgaaata tgtcccggtc ggcgaagacc aggtgccgca
     1321 cctcgagttc gcgcgcgagg tggcgcggcg ctttaatcat ctctatggcc gcgagccggg
     1381 cttcgaggac aaggcgaaga ccgcggtcaa gaagatggga acgaagaaag cccggctcct
     1441 gaacgagctg cgcaccaagt tcatcgagca gggcgacgcc ggcgcgctgg cgcgcggcca
     1501 ggcgctcctc aaggagcagc agaacctgtc gcgcggcgac cgcgaacgcc tctacggctg
     1561 gctcgaggga acgggcaaga agatcctggt cgagccggag gcgctcctca ccgaggcgtc
     1621 caagatgccc gggctcgacg gacaaaaaat gtcgaagtcc tacggcaaca cgatctcgct
     1681 gcgcgaggag ccggaggtcg tggcgaagaa gatcaagacg atgccgaccg acccggcgcg
     1741 ggtaaagcgc tccgatccag gaaaccccga gaagtgcccg gtctgggagc tgcacaaagt
     1801 ctactcggac gcggcgcgca aggactgggt gatgaggggt tgcactaccg ccggcatcgg
     1861 ctgcctggag tgcaagcagc cggtcatcga cgccatcaat ctcgagctcg cgccgatgcg
     1921 cgccagagcg cagccttacc tggaggattc tgcgcttgtg aagaacatca tcgccgatgg
     1981 ctgcgaaaag gcgaagcgcc tggctgccga gacgatgcgc gatgtgcgcg aagccatggg
     2041 attaagctat tcatgagggc caaggtctac ggcgaacccg ttatcgagct gccgcatgac
     2101 ctctatatcc cgcccgacgc gctggaagtg gtgctcgagg cgttcgaagg gccgctcgac
     2161 ctgctgctct atctcatccg caaggagaac ctcgacatcc tcgacatccc gatggcgccg
     2221 cttacgcgcc agtatctcga gtatgtcgac atgatgcgct cgacaaatct cgagctcgcc
     2281 gccgaatact tggtcatggc ggcgatgctg atggagatca agtcgcgcct gctgctgccg
     2341 cggccaccgg ccgccgagcc gatcgaggac gacccgagag cggagctggt gcgcaggctc
     2401 ctggagtacg agcgcatgaa aaaggcggcc cagggcctcg acgagctgcc gcagctgggc
     2461 cgcgacgtga tcgccatctc ggtgtggatc gagaagcaag tgtcgcagcg ccttcccgaa
     2521 gttaacgcga gagacttggc ggaagcctgg cgcgggctgc tgcaccgggc gcggctttcg
     2581 gcgcatcatc gcgtaagccg cgaagagctc tcggtgcgcg cacatatgag ctcgatcctg
     2641 cgcgcgctgc gcgagcgacg cgtggtggag ttcggcgagc tgttcgagcc ccagcgcggc
     2701 atcgcggtac tggtcgtcac cttcctcgcg ctgctcgagc tcgcgcgtga gcttctgatc
     2761 gagatcacgc agtctgcggg cttcggcccg atttacgtaa aattggcgca tgctcaatcc
     2821 gcctgaggcg aagcggatcc tggaagcggc gcttctcgcc tcgcaggaac cggtcagcgc
     2881 gatcgacttg aagcgcctgt tcgacgacga gctgggcgtc gacaccatcc gcagcctcct
     2941 cgccgagctt gccgacgaat ggcgcggccg ggcggtcgag ctcgtaagcc tttccagcgg
     3001 ctggcgtttt cagacgcgcc cggagttcca gccgtacctc gagcgtctgc tcccggagcg
     3061 gccgccgcgc tattcgcggg ccgtgatgga gacgctcgcg atcattgcct accggcagcc
     3121 ggtgacccgc ggggacatag aagacgtgcg cggcgtcggc gtctccacgc agatcatcca
     3181 ggcgctcgag actcggggct ggatcgacgt ggtcggccac cgggaaaccc ccggccggcc
     3241 ggcgctttac gccaccacca ccaagttcct ggacgacttg gggctgcgat cgctggagga
     3301 gctgccaccg ctcgaggaga tcgccaggac cctccaactg gaggtcgtct ccactggcaa
     3361 agccgaggtc cgggaacccg aagcacagcc cgccaccggc accgacggct gagcggatcc
     3421 agaagctgct cgctggcgcc gggctcgcct cgcggcgcga gatcgagagc tggatctccg
     3481 ccgggcgggt gagcgtaaac ggacgcctgg ccaagctcgg cgaccgcgca ggtcctgcgg
     3541 attccatact tctggatggg aagcccgtcg tcgccgcggc gcgcacgcag ccgcgcgtgc
     3601 tgctctacca caagcccgtc ggcgagctcg tcacacgcag cgacccgcag ggcaggccca
     3661 cggtgttctc caacctgccg cccgggcgct ggatctcggt ggggcgactc gacctgaaca
     3721 gctccggtct gctgctcttc actgactctg gcgagcttgc aaaccggctg atgcacccga
     3781 gatatggcct ggagcgagaa tactccgcgc gaattcgcag accgctcagt gccgcggagg
     3841 aaaagcggct tctgcagggc atcccgctgg acggtttgct tgctcgcttc gatcgcatcg
     3901 aggagatgaa atccggtgcg ggcaccaatc ggtggtatcg ggtggtgctt cgcgagggcc
     3961 gcaaccgcga ggtgcggcgc attttcgagg cgctcggcca ccccgtgagc cggctggtcc
     4021 gaatgcgtta cggcccgatc gagttgcccg cggatctacc gcccaaaagc tggcgagagc
     4081 tgccaaatcc ggctatactt caaaggcttt agagagatgg ggtaaggaat gggctgcggg
     4141 cccatttttt gttttgagcc tcgaaaaagt cgtggagccg gcggtgaccg ggctgggctt
     4201 cgagctggtc gacgtgcagg cgtcgaacgg cgggcgca
//
LOCUS       DASTPD010000339         6052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1209087, whole genome shotgun sequence.
ACCESSION   DASTPD010000339 DASTPD010000000
VERSION     DASTPD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209087"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFR57_11485"
     CDS             <1..881
                     /locus_tag="VFR57_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU0260024.1"
                     /translation="PKRTARALLDIEDPKVVPIIRDPGKASEDSDAVFYFPGCGSERL
                     FSQVGLATQAMLYAVGTQCVLPPGYLCCGYPQTAAGDSDKGERITVQNRVLFHRLATT
                     LNYLDIRTVIVSCGTCLDQLEKYDFGSIFPGARLLDIHEYLLEKGVKVNDASGVRYMY
                     HDPCHTPIKTAKPLSVVNGLMGANPGDEIRLSERCCGEAGTLAMTRPDVSTQVRFSKE
                     ESLKKSMKEMKGDGPVKVLTSCPSCLQGLARFEDDTGTQADYIVVELARRMLGEDWLA
                     QFVAKANAGGIERVLL"
     gene            878..1227
                     /locus_tag="VFR57_11490"
                     /pseudo
     CDS             878..1227
                     /locus_tag="VFR57_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
     gene            1211..1912
                     /locus_tag="VFR57_11495"
     CDS             1211..1912
                     /locus_tag="VFR57_11495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0260025.1"
                     /translation="MQAGNRAQTVRRVFDSVAERYDVMNDLMSLGLHRAWKTVAIAVA
                     RPRPGERVLDIATGSGDLALSLSRKVSPGGEVWISDINRRMLERGRDRLLDRGLALRA
                     VQCDAERLPFPAAHFDCVTVGFGLRNMTRKDVALAEMARVLKPGGRLVVLEFSKVWKP
                     LERAYEWYSFKVLPWLGDLVAGDADAYRYLAESIRAHPGQAALAELLRSAGLADVEVF
                     NLAGGVVAIHRGYRF"
     gene            1941..2756
                     /locus_tag="VFR57_11500"
     CDS             1941..2756
                     /locus_tag="VFR57_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEU0260026.1"
                     /translation="MSKWLLASAVLVFSGIFFATEVEARRLGGGRTIGAQRNVTAPPA
                     QTPAKPAQQQAAPAQQGAQQGAPASTGSRWAPILGGLALGGILGYLFGGSGLLGFLLL
                     ALLAIAAVVGVRALMHRRTGAPQPVQFAGMRETVSVPVRDELPATSNARLPAGFDASG
                     FLRAAKHNFLRLQAANDVGRLEEIREFTTEELYGQLKSELGHRGETEVLGLEAQLLEI
                     ATEGDRHWASVHFSGAVREAHGAAAEPFAEVWNLVKPADGSSGWLLAGIQQMH"
     gene            2843..3370
                     /locus_tag="VFR57_11505"
     CDS             2843..3370
                     /locus_tag="VFR57_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260027.1"
                     /translation="MLAGEPWARLRLLPFAGETVELRAPPLPALRLRILEGGTVEAGD
                     AEPSLTMTFKPDLVFALARGEEHVARAVEVQGNARLGAEILALVRHLRWDAEEALSRL
                     IGDVAAHRLVDAGRAFAAWHVDGARRLIAALGDYLTDEKELLARRAELEALGESVARL
                     RDGIARLDKRIEQLD"
     gene            3363..4865
                     /gene="ubiB"
                     /locus_tag="VFR57_11510"
     CDS             3363..4865
                     /gene="ubiB"
                     /locus_tag="VFR57_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEU0260028.1"
                     /translation="MIRVGRLARILAVGLRFGLHEFVPRFRSNIVVRALAGRTREPRG
                     QRLREALETLGPIYVKFGQVLSTRRDLLPVDIADELARLQDQVPPFRSELAMAEVERS
                     LGRKLDEVFAYFEESPVASASIAQVHLARLHGGAEVAVKVLRPGVEAAVERDLALLET
                     GAALVERLWADGKRLRPRDVVAEFARHVDNELDLMREAASASQLGRNFAASPLLIVPA
                     VYWDLCAQRVMVMERMHGTPISQVQVLREKGIDIPALARAGVEIFFTQVFHHGFFHAD
                     MHPGNIRVADDGRYIALDFGIMGTLTEVDKNYLAQNFLAFFNRDYRRVAQAHLDAGWV
                     PAGTRIDEFEAAIRAVCEPVFARPLKEIYFGKLLLRLFQTSRRFNVEVQPQLLMLQKT
                     LLNIEGLGRELDPDLDLWQTAKPFLERWMREQVGWRGFVRTIRHEAPFWAATLPQLPR
                     LVHRALAEDRLGALQLAVEGLARQNARRNRLLTVLIFVVALAALVAIWQA"
     gene            4963..5124
                     /locus_tag="VFR57_11515"
     CDS             4963..5124
                     /locus_tag="VFR57_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260029.1"
                     /translation="MVQNAYKVTLVSAFALLVLGLYWKRSTVDGMVVGSLCGRQMPRR
                     PHAAHPRGH"
     gene            5099..>6052
                     /locus_tag="VFR57_11520"
     CDS             5099..>6052
                     /locus_tag="VFR57_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU0260030.1"
                     /translation="MQRTPEGTEFVAARSSTLARILFLVYVLLVAYASLYPLSGWRNV
                     GISPFAYLVEPWPRYVTAFDIVANVAGYLPFGFLATAVLHPRLRGVRAFGAAIAGATV
                     LSLVLEAGQTYLPARVPTTLDVLCNIGGAALGAALGQHFTLLIGGGPLSRWRAAAFVP
                     GAGMDFGLMLIGLWLFIQLNPATLLFGAGDLRDLLAPLEGRERRPEFFVSIEAFTTAA
                     NLVAIALILAALASPGRPVRSLVLGTVLAALTVKTAAVAILLRAENVFTWLTHGAQLG
                     LVIGVAVALAAVALPRTVRLVVAAVLLMAATVLVNLAPPNPY"
BASE COUNT          941 a         1979 c         2010 g         1122 t
ORIGIN      
        1 tgccgaagcg gaccgcacgc gcgttgctcg acatcgaaga tccgaaagtt gtgccgatca
       61 tccgcgatcc gggcaaagcc agcgaagact ccgatgcggt gttctatttc ccgggctgcg
      121 gctctgagcg cctgttctcc caagttggac tcgctacgca ggcaatgctc tacgcagtgg
      181 gtacgcagtg cgtgctgccg ccgggctacc tgtgctgcgg ctacccgcaa accgcggccg
      241 gcgactccga caagggcgag cgcatcacgg tgcagaaccg ggtgctcttt caccgcctcg
      301 ccacgaccct caactacctc gacatccgca cggtcatcgt ctcttgcggc acctgcctcg
      361 accagctcga gaagtacgac ttcggctcca tcttcccggg cgcgcgcctt ctcgacatcc
      421 acgagtacct gctcgagaaa ggcgtcaagg tgaacgacgc gtccggcgtc cggtacatgt
      481 accacgaccc ctgccatacg cccatcaaga cagcaaagcc gctcagcgtc gtgaacggcc
      541 tgatgggcgc aaaccccggg gatgaaatcc ggctctccga gcgctgctgc ggcgaggcgg
      601 gcacgctcgc gatgacccgg ccggacgtgt ccactcaagt caggttcagc aaggaagaaa
      661 gcttgaagaa aagcatgaaa gaaatgaaag gcgacggccc ggtaaaggtc ttgacctcat
      721 gtccctcgtg cctgcagggt ctcgcacgct tcgaggatga caccgggacg caggcggact
      781 acattgtggt cgagctcgca cgccgcatgc tcggcgagga ctggcttgcg cagttcgtcg
      841 ccaaggcgaa cgcgggcggc atcgagcggg ttcttctatg agcgagcgga ttgcgaccgc
      901 gatcactctg caccagaagt cgcgcgtgat ggagtttccg acggtcgcag cttccgcctg
      961 ccgtacgagt tcctgcgcgt gtactcgcct tcagccgaag tgcgcggtca cgggccgggg
     1021 caggaggttc tgcaggccgg aaagcgtaac gtcgagatcc gctcgctcga gccggtcggc
     1081 tcctatgccg tgcagccaca gttctccgac gggcacaata ccggaatcta ttcctgggag
     1141 tatctctacg agctcggaga gaaccaggac aagctctggt cgaaatatct gaacgagctt
     1201 aaagccgccg gtgcaagcag ggaatagagc gcaaacggta aggcgcgtct tcgactcggt
     1261 tgcggagcgc tacgacgtca tgaacgacct catgtcgctc gggctgcacc gcgcgtggaa
     1321 gacggtcgcg atcgccgtcg ctcgcccgcg gccgggcgag cgcgtgctcg acatcgccac
     1381 cggcagcggc gatctcgcgc tatctctgag ccgcaaggta agccccggcg gcgaggtatg
     1441 gattagcgac atcaatcggc gcatgcttga gcgcggccgc gaccgcctcc tcgatcgcgg
     1501 actcgcgctg cgggcagtgc agtgcgacgc cgagcggctg ccatttccgg cggcgcattt
     1561 cgattgcgtc acagtggggt tcggcctgcg caacatgacg cgcaaggatg tcgccctcgc
     1621 cgagatggcg cgcgtcctga agccgggcgg gcgtcttgtc gtcctggagt tctcaaaggt
     1681 gtggaagccg ctcgagcggg cgtacgagtg gtattcgttc aaggtgttgc catggctcgg
     1741 cgaccttgtc gccggcgatg ccgacgccta ccgctacctc gcggaatcga ttcgcgcgca
     1801 tcccggccag gctgcgctcg ccgagctcct gcgctcggcg ggcctcgctg atgtcgaggt
     1861 gtttaacctt gctggcggag tggttgcgat tcatcgcggg taccgttttt gatagcattc
     1921 acggctcgga ggaacgactt atgagcaagt ggcttctcgc atcggcagtt ctcgtgttca
     1981 gcggaatttt cttcgccacc gaagtcgaag cgcggcgcct cggcggcggc cgcaccatcg
     2041 gcgcgcagcg caacgttaca gctccgcctg cgcagacgcc ggccaagccg gcgcagcagc
     2101 aggcagcgcc ggcacagcag ggcgcgcagc agggagcccc tgcttcgacg ggtagccgct
     2161 gggcgcccat cctcggcggg ctcgcgcttg gcggaattct cggctacctc ttcggcggca
     2221 gcggccttct cggcttcctg ctcctcgcgc ttctcgcgat cgccgcggtc gtcggcgtgc
     2281 gcgctttgat gcaccggcgc actggtgcgc cgcaacccgt tcaattcgcc ggtatgcgcg
     2341 aaacggtttc cgtcccggtg agagacgagc tgccggcaac ttcgaacgca agactgcccg
     2401 ccggcttcga tgcgtcgggc ttcctgcggg cggcgaagca caacttcctc cgcctacagg
     2461 ccgcgaacga cgtcggccgg ttggaggaaa tccgcgaatt cacgaccgaa gagctctacg
     2521 ggcagctgaa gagcgagctc ggtcataggg gtgagaccga ggtccttggc ctcgaggcgc
     2581 agctactgga gatagcgact gagggcgacc gccattgggc gagcgtgcat ttttcaggcg
     2641 cagtgcgcga ggcgcacggc gcggcggccg agccgttcgc tgaagtctgg aacctcgtga
     2701 agcccgccga cgggtcgagc ggctggctgc tcgcgggcat ccagcagatg cattgacaag
     2761 taccgaagat cggggtctca ccccgatttt ccccgtctct gaggccaccc ccgcggtggc
     2821 cttttgtttt ctgctgaacc gcttgctcgc tggcgaaccc tgggcgcggc tgcggctctt
     2881 gcccttcgcc ggcgagaccg tcgagctgcg cgcaccgccg cttcccgcgc tacgcttgcg
     2941 catccttgag gggggaaccg tggaggcagg cgacgcagaa ccgagcctga cgatgacgtt
     3001 caagccggac ctggttttcg cgcttgcccg cggggaggaa cacgtcgcgc gcgcggtgga
     3061 ggtgcaaggg aatgcccggc ttggcgcgga gatcctcgcg ctcgtacggc acctgcgttg
     3121 ggacgcggag gaagcgcttt cgcgcttgat cggcgacgtc gcggcgcatc ggcttgtgga
     3181 cgccggccgc gcttttgcgg cctggcacgt ggatggggcg cgccgcctga tcgccgctct
     3241 tggcgactac ctgaccgacg agaaggagct cctcgcgcgc cgcgcggagc tcgaggcgct
     3301 cggcgagtcg gtcgcgcggt tgcgggacgg catcgcaagg ctggacaaac ggatcgagca
     3361 gcttgattag ggttggccgg ctcgcgcgca tcctggcggt cggcctgcgc ttcggtctgc
     3421 atgaattcgt cccccggttt cgcagcaata tcgtggtgcg cgcgctcgcc ggccgaaccc
     3481 gcgagccgcg cggccagcgg ctgcgcgagg cactcgaaac gctcggtccg atttacgtca
     3541 agttcggcca ggtgctgtca actcgacggg acctccttcc ggtcgacatc gccgacgagc
     3601 tcgcaaggct ccaggaccag gtgccaccgt tccggtccga gctcgcgatg gccgaggtgg
     3661 aacgctcgct tggcaggaaa ctcgatgagg tcttcgctta ctttgaagag agccctgtag
     3721 cgagcgcctc gatagcgcag gtgcatctgg cgcggctgca cggcggcgcc gaagtcgcgg
     3781 taaaggtgct gcggcccggc gtcgaggccg ccgtggagcg tgacctcgcc ctgctggaga
     3841 ccggtgccgc gctggtggag cggctttggg ccgacggcaa gcgcctgcgt ccaagggacg
     3901 tggtcgccga gttcgcgcgc catgtcgaca acgagctcga cctcatgcgc gaggccgcga
     3961 gcgcgagcca gctcggacgt aattttgcag cgtcgccgct cctcatagtg cccgcggtgt
     4021 actgggacct ttgcgcgcaa cgcgtgatgg tgatggagcg gatgcatggc acgccgatct
     4081 cgcaagtgca ggttctgcgg gaaaagggaa tcgacatccc ggcgctggcg cgcgccgggg
     4141 tcgagatttt ttttacccag gtcttccacc atggtttctt ccatgcagac atgcaccccg
     4201 gcaacatccg ggtggcggac gatgggcgct acatcgccct cgacttcggc atcatgggca
     4261 cgcttaccga ggtggataag aactacctcg cgcagaactt ccttgcattc ttcaaccgcg
     4321 actaccggcg agtggcgcag gcgcaccttg atgcaggctg ggtgcccgcg ggcacgcgta
     4381 tcgacgagtt cgaggccgcg atccgtgccg tgtgcgagcc ggtgttcgca cggccgctga
     4441 aggaaatcta cttcggcaag ctgctactgc gcctgttcca gacctcacgg cgcttcaacg
     4501 tggaagtgca gccgcagctt ctgatgctgc agaagacgct cctcaacatc gaaggcctgg
     4561 gtcgggagct cgatcccgac ctcgatctct ggcagacggc caagcccttc ctcgagcgtt
     4621 ggatgcgcga gcaggtcggc tggcgcgggt tcgtgcgcac catccgtcac gaggcgcctt
     4681 tctgggcggc caccctgcca cagctacccc ggctcgtgca tcgcgccctt gccgaagacc
     4741 gcctcggcgc tctgcagctc gccgtcgaag ggctcgccag gcagaacgcc cggcgcaacc
     4801 gcctgcttac cgtgctgatc ttcgtcgtag cgctcgcggc gttggtcgcg atctggcagg
     4861 cgtgaaagcg gcgctatgat ctggcgcgca tgctgatcaa gttcgatttt cggccttgcg
     4921 gtgatggtgt ttgcgctact tagcgatgcg agtatctacc gaatggtgca gaacgcctac
     4981 aaggtgacgc tggtgtcggc cttcgcgctg ctcgtgctcg gcttgtactg gaagcgctcc
     5041 accgtcgacg gcatggtcgt cggttcgctg tgcggccgtc agatgccgcg gcggccccat
     5101 gcagcgcacc cccgagggca ctgagttcgt tgccgcgcgc agctcgacgc tggcgcgcat
     5161 cttgttcctc gtctatgtct tgctggtcgc ctatgcgtcc ctctaccccc tatccggttg
     5221 gcgaaacgta gggatttcgc cgttcgccta tctggtcgag ccctggccgc ggtacgtcac
     5281 cgcgttcgac atcgtggcga atgtcgccgg ctatcttccc ttcggtttct tggcgaccgc
     5341 ggttctgcac ccacgcctgc ggggcgtgcg ggccttcggg gcagcgatcg ccggggccac
     5401 cgtactgtca ctcgtccttg aagctgggca aacctacctt cccgcgcgtg tcccgaccac
     5461 cctcgacgtg ctttgcaaca taggcggggc ggcgctcggt gcggctctcg gccagcattt
     5521 caccttgctc atcggcggcg ggccactgtc ccgctggcgt gcggcggcgt ttgtgcccgg
     5581 ggccggcatg gacttcggcc tgatgctgat cgggctgtgg ctgttcatcc aactgaatcc
     5641 cgcgaccctg ctcttcggcg cgggcgattt gagggacctg cttgcgccgc tcgaagggcg
     5701 cgagcggcgc ccggagttct ttgtgtccat cgaggcgttc accacggccg caaacctggt
     5761 cgccatcgcg ctgattctcg cggcgctcgc ctcgcctggg cgcccggtgc gcagcctagt
     5821 gctcggcaca gtcctggcgg cgcttacggt gaagacagcc gccgttgcga ttctcctgcg
     5881 cgccgaaaac gttttcacat ggcttacgca cggagcgcag ctcggcctcg tcatcggcgt
     5941 cgcggtcgcg ctcgccgccg tggcgctgcc ccggaccgta cggttggtcg tggccgctgt
     6001 ccttctgatg gccgccaccg tgttggtgaa tctcgcgcca ccgaatccct ac
//
LOCUS       DASTPD010000340         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_391435, whole genome shotgun sequence.
ACCESSION   DASTPD010000340 DASTPD010000000
VERSION     DASTPD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391435"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /gene="ptsP"
                     /locus_tag="VFR57_11525"
     CDS             complement(<1..1280)
                     /gene="ptsP"
                     /locus_tag="VFR57_11525"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU0260031.1"
                     /translation="MNFVLHGYPVSGGITVGYAHLVSTARLEVAHYEVAEQAVDAEVA
                     RFDHAMTAAQEELAALKAHIPPGSPAEFEAFLDLHRMILNDSALAQAPRELIRKRRAN
                     AEWALVQQMERLVDRFEEIDDPYLRERKADVQQAVERVLKSLMGGQALSPPAISEEEK
                     LIVVAHDLSPADMILFKRHRFAGFVTDVGGVTSHTAIVARSLGIPAIVGLHHARDTIA
                     EGEVLIVDGTQGVLIVNPDPLVLAEYRARQAELKAERKRLARLKSTPAATVDGTPIEL
                     FANIELPQDMPEVLEAGAEGVGLFRTEFMFLNRKDLPGEEEQFEAYREVAQAMKGRPV
                     VLRTLDLGADKALNGSGEQHTMPNPAMGLRAIRFCLAEPQIFLTQLRAILRASRYGKV
                     RMLLPMLAHAHEIDQTLTLIAQAKAQLDERDQPY"
     gene            complement(1277..1546)
                     /locus_tag="VFR57_11530"
     CDS             complement(1277..1546)
                     /locus_tag="VFR57_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU0260032.1"
                     /translation="MPSANVEIVNKLGLHARAAAKLTHLAGGFQCEIWISRSGRRVNA
                     KSIMGVMMLAAGQRSTVLIEAEGPDADQAIAALTKLIAEKFGEGE"
     gene            complement(1539..1943)
                     /locus_tag="VFR57_11535"
     CDS             complement(1539..1943)
                     /locus_tag="VFR57_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEU0260033.1"
                     /translation="MIGVLIVTHGSIGEALLTSASQILGAAPQQVATLSVWRQDDPDD
                     LVLRARELLEGLDAGDGVLVLTDIFGATPGNVVSRLLEDGRIEGVSGASLPMLLRVLT
                     GRNGSLSAAVQRALSGGAEGVVHMNSDRCCDA"
     gene            complement(1940..2881)
                     /gene="gshB"
                     /locus_tag="VFR57_11540"
     CDS             complement(1940..2881)
                     /gene="gshB"
                     /locus_tag="VFR57_11540"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEU0260034.1"
                     /translation="MQFLFIIDPLDSLKAYKDTSVAMMRAAQARGHAVWVCEQPSLHW
                     LDGRVAAAAQRISLVDNDEDWYRGHETEKRALADFDAVLMRKDPPFDLEYVVSTWLLS
                     AAVREGARVFNAPDAIRDHNEKLAIAEFARFAAPTLVSREPERIQAFIDAERDIVVKR
                     LDVMGGENVFRVRADDPNRNVIVETISLGGTRSVMAQRYIPEIAQGDKRVILIDGKVV
                     PHCLARIPKSGDSRGNLAQGARGVAQPLSKRHKEIAAAVGAIVAKRGLLVVGLDVIGD
                     YLTEVNVTSPTCFREIQDQTGFDVSGMFLDALEKKVA"
     gene            complement(2894..3421)
                     /locus_tag="VFR57_11545"
     CDS             complement(2894..3421)
                     /locus_tag="VFR57_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0260035.1"
                     /translation="MEAHDPHQSFIKTPKQLVVVILLAFLVPIIGIILLVQLVLSGAR
                     PDPQALAPEAVERRIQPVARLEFGPPEGVAPKPAAAAPVKAGAVAAAGKPGAADGKAV
                     YDKVCVACHQVSVAGSPRLGDKAAWAPRIQTGMDSLLQSVVKGKGAMPPRGGNPSLSD
                     AEVRAAVEFMVGQAK"
     gene            3495..3571
                     /locus_tag="VFR57_11550"
     tRNA            3495..3571
                     /locus_tag="VFR57_11550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3529..3531,aa:Arg,seq:ccg)
BASE COUNT          620 a         1271 c         1235 g          673 t
ORIGIN      
        1 tcgtatggct ggtcccgctc gtcaagctgc gctttcgcct gcgcgatgag tgtgagagtc
       61 tgatcgatct catgcgcgtg ggcgagcatc ggcagcagca tgcgcacctt cccgtagcgc
      121 gaggcacgca ggatggcgcg caactgcgtg aggaaaatct gcggctcggc gaggcagaag
      181 cggatcgcgc gcagccccat cgccggattg ggcatggtgt gttgctcgcc gctgccgttc
      241 agcgccttgt cggcacccag atccagcgtg cgcagaacca ccggccggcc tttcatcgcc
      301 tgcgcaacct cccggtaggc ctcgaactgc tcttcctcgc ccggcaggtc cttgcggttg
      361 aggaacatga attccgtgcg gaaaagcccc acgccctcgg cgcccgcttc gagcacctcg
      421 ggcatgtcct gcggcagctc gatattcgcg aaaagctcga tcggggtacc gtccaccgtg
      481 gcggcggggg tggacttaag tcgcgccagg cgttttctct ccgctttgag ctccgcctgg
      541 cgcgcgcggt actcggcgag aaccaggggg tccggattga cgatcaacac accctgcgtg
      601 ccgtcgacga tcaagacctc accctcggca atggtgtcgc gcgcgtggtg caggcccacg
      661 atcgccggga tgccgagcga gcgcgccacg atcgcggtgt gcgaggtaac gccgcccacg
      721 tcggtgacga agccggcgaa gcgatggcgc ttgaagagga tcatgtcggc gggggagaga
      781 tcgtgcgcga ccacgatgag cttctcctcc tcggagatcg ccggcggact gagcgcctgg
      841 ccgcccatga gcgacttcag cacgcgctcg accgcctgct gcacatccgc cttgcgctcg
      901 cgcaggtacg gatcgtcgat ctcctcgaag cgatccacca ggcgctccat ttgctgcacc
      961 agcgcccact cggcattggc gcgccgcttg cggatgagtt ccctcggtgc ctgcgcgagc
     1021 gccgagtcgt tgagaatcat gcggtgcagg tcgaggaagg cctcgaactc ggcgggcgat
     1081 ccgggcggaa tgtgggcttt cagcgcggcg agctcctcct gcgccgcggt catggcgtgg
     1141 tcgaagcgcg ccacttcagc atcgaccgcc tgctcggcaa cctcgtagtg cgcgacctcg
     1201 agccgggcgg tggagacgag atgggcgtag cccacggtaa tcccgccgga gaccgggtaa
     1261 ccgtgaagaa cgaagttcat tccccctcgc cgaacttttc cgcgatgagc ttggtgagcg
     1321 ccgcaatcgc ctgatccgcg tcggggcctt cggcctcgat cagcaccgtg ctgcgctggc
     1381 ccgcggcgag catcatcacg cccatgatgc tcttcgcgtt cacgcgcctg ccgctgcgtg
     1441 agatccagat ctcgcattgg aagcccccgg cgaggtgcgt gagcttggcc gcggcgcgtg
     1501 cgtgcagccc gagcttgttg acgatctcga cgttcgcgct aggcatcgca gcagcggtcg
     1561 ctgttcatgt gcaccacccc ttccgcgccc ccggaaaggg cacgctgcac ggcggcagag
     1621 agcgatccgt tgcggcccgt aagcacgcgc agcagcatcg gcagcgaggc gcccgacacc
     1681 ccctcgatgc ggccgtcctc gagcaggcgc gaaactacat tgccgggcgt ggcgccgaag
     1741 atgtcggtca gcacgaggac cccgtcgccc gcatcgaggc cttcaagcag ctcgcgcgca
     1801 cgcagcacca ggtcgtcggg gtcgtcctgg cgccagacgc tgagcgtggc tacctgctgc
     1861 ggcgcggcgc ccaggatctg gctggcgctg gtcagcagcg cctcgccgat gcttccgtgg
     1921 gtgacgatca ggacgccgat catgcgacct tcttctccag ggcgtccaag aacatgcctg
     1981 acacgtcgaa gccggtctgg tcctggatct cccggaagca ggtagggctg gtgacgttga
     2041 cctcggtcag atagtcgccg atcacgtcga gtcccaccac cagcaacccc ctttttgcca
     2101 cgatcgcacc caccgccgcg gcgatttcct tgtgccgttt gctcaatggc tgggcgacgc
     2161 cgcgcgcgcc ctgcgcgaga ttgccgcgcg agtcgccact cttcgggatg cgcgcgaggc
     2221 aatgcggcac gactttcccg tcgatcagga tcacgcgctt gtcgccctgc gcgatctcgg
     2281 gaatgtagcg ctgcgccatt acgctgcgcg tgccgcccag gctgatcgtc tcgacgatca
     2341 cattgcgatt tgggtcgtct gcgcgcacac gaaacacgtt ttcgccgccc atgacgtcga
     2401 ggcgcttcac cacgatgtcg cgctcggcgt cgatgaatgc ctggatccgc tcgggctcgc
     2461 gcgacaccag cgtcggggcg gcaaaccgcg cgaactcggc aatcgcgagc ttctcgttgt
     2521 ggtcgcggat cgcgtccggg gcgttgaaca cgcgcgcgcc ctcccgcact gcagcggaca
     2581 gcagccacgt gcttaccacg tactcgagat caaacggcgg gtcttttcgc atcagcaccg
     2641 cgtcgaaatc cgcgagcgcg cgcttctcgg tttcgtggcc ccggtaccag tcctcgtcgt
     2701 tgtcgacgag cgagatacgc tgggcggccg cggcaacgcg tccgtccagc caatgcaggc
     2761 tcggctgctc gcacacccag accgcgtggc cgcgcgcctg cgcggcgcgc atcatcgcga
     2821 cgctcgtgtc cttgtacgcc ttgagcgaat caagcgggtc gatgatgaag aggaactgca
     2881 gggcacaccc gtcttacttg gcttgcccga ccatgaattc gaccgcagcg cggacctccg
     2941 cgtcggacag cgacgggttg ccgccgcgcg gcggcatcgc gcccttgccc ttgacgaccg
     3001 actgcaggag cgaatccatg ccggtctgga tgcgcggcgc ccaagccgcc ttgtccccga
     3061 ggcgcggcga gcccgcaacc gaaacctggt ggcacgcgac gcagaccttg tcgtatacgg
     3121 ctttgccgtc ggcagcccca ggcttgccgg cggcggcaac ggcaccggcc ttcacgggcg
     3181 ccgctgcagc gggctttggg gcgacgcctt caggcgggcc gaattcgaga cgcgcaaccg
     3241 gctggatgcg gcgctcgacc gcctcgggcg cgagcgcctg cgggtcgggg cgcgcaccac
     3301 tcagcacgag ctgcaccaga agaatgatgc cgatgattgg caccaggaac gcaagcaaga
     3361 tcacgacgac caattgcttg ggcgtcttga tgaatgactg gtgcgggtcg tgcgcttcca
     3421 cagtgcgctg tgtagtcttg taaaggcatg attttagctt gagagtctgc tattctgcgc
     3481 ctccgttttt cgccgcgccc gtagctcagc ggatcagagt attggcctcc gaagccaagg
     3541 gtcgtgggtt cgaatcccgc cgggcgcgcc atcaagttat gggtgtattc cggttacatg
     3601 ggtaacggtt tataccggag acataggtaa cactctcgcc tcgaacgggt taaccaccgc
     3661 cggtctcatc atcaaagaag cccaagtcgt aggcatggac ctgacgatcc gaatcccttc
     3721 gtcgatcgct ttgatgccga ccgctgaccg gcaaacacgt gtgaggggtt gacccgtttg
     3781 cagtgtaggc agatgcgtc
//
LOCUS       DASTPD010000341         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1615926, whole genome shotgun sequence.
ACCESSION   DASTPD010000341 DASTPD010000000
VERSION     DASTPD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615926"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1298
                     /locus_tag="VFR57_11555"
     CDS             108..1298
                     /locus_tag="VFR57_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0260036.1"
                     /translation="MAELPRLVAHARKRASGFARILSEVNPSIVRSRAALAKLPVTRK
                     SDLATLQKELPPLGGLNATPVEKLGKLFISPGPIYDPEGRGKDWWRTARGLFAGGFRA
                     GDRVLNTFAYHFTPAGSMLESGATAIGCTVIPSGVGQTEMQVAAIRDLKVNAFVGTPS
                     FLKLIVEKADELKADISSLEKAHVGAEYLPPSLRSSLRDRGIRVSQTYASADLGLIAY
                     ESVMPDGTVNEGMILDERLILEIVRPGTGDPVATGEVGEVVITTFNKDYPLIRFATGD
                     LSAVLPGTSPCGRTNVRIKGWMGRADQSTKVRGMFVTPRQVNDIVRRHPEIVRARLVV
                     EGEAGDDRMTLKCEMRERLQGIAEAIVASIREVTKLRGDVQLVEPGSLPNDGKVIEDT
                     RKYG"
     gene            complement(1311..1928)
                     /locus_tag="VFR57_11560"
     CDS             complement(1311..1928)
                     /locus_tag="VFR57_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEU0260037.1"
                     /translation="MATSIDWYFDFVSPYSYICLHRLPELDADIDFKPVLFAGLLQHW
                     GQKGPAEISAKRKWTYRWCTWWAAELGIPFRFPAEHPFNPLQHLRLALACESRPEAVK
                     RIFDSLWMSGGNAGDPARFQALCRELNVDPAALADEQIKSLLRRNTEHAVARGVFGVP
                     SFVVDGEVFWGADAVDFVKAFLKDRSLLSSDEMRRLDDLPVGAAR"
     gene            1927..2730
                     /locus_tag="VFR57_11565"
     CDS             1927..2730
                     /locus_tag="VFR57_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU0260038.1"
                     /translation="MAVRIEKKGKVWTVIHSRPEARNAMDPESADALVSAFQSFDKNP
                     DAAVAVLWGDGGAFCAGWDLKYGASLMKDPTPIHEIDYPLDDRKPIPRGPLGPTRLEL
                     DKPVIAAVAGPAVAGGFELALWCDVRVMEQSAYFGVYCRRWGVPLIDGGTVRLPRIVG
                     MGRALEIILTGRKVPAEEALHIGACEHVVPDGKSREFAEAMAHEIARFPQACTRADRR
                     SAYAQQGLSLREAMRKEWYNGIPAFKAEGAAGAARFSSGKGRHGDFGNI"
     gene            complement(2692..2886)
                     /locus_tag="VFR57_11570"
     CDS             complement(2692..2886)
                     /locus_tag="VFR57_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEU0260039.1"
                     /translation="MKTPCIKVCQMDPVRGVCIGCCRTLDEIARWGAMTETEQTAVMR
                     ELPARRKKLDIAEIAVPPLA"
     gene            complement(2883..3785)
                     /locus_tag="VFR57_11575"
     CDS             complement(2883..3785)
                     /locus_tag="VFR57_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU0260040.1"
                     /translation="MLDAIKIVEVGPRDGLQNEPGQVSTDVKVELIERLAGAGLPAVE
                     ATAFVSPKWIPQMADHTEVMERIRRRPGVSYPVLTPNLKGFEAARAAGATDVAIFGAA
                     SEAFSKKNINCSIAESLERFEPIALESRKSNIRVRGYVSCVLGCPYEGEIKPDKVAEV
                     AGALYDMGCYEVSLGDTIGTGTPGKTKAMIEACARRVPLERLAGHYHDTYGQALANIY
                     ASLEMGVATFDASVAGLGGCPYAAGASGNVATEDVIYMLNGLGVRTGVDLDQLVEIGQ
                     WICGLLNRKPASKAALAISAKKKK"
     gene            complement(3778..5733)
                     /locus_tag="VFR57_11580"
     CDS             complement(3778..5733)
                     /locus_tag="VFR57_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEU0260041.1"
                     /translation="MFAKILIANRGEIACRVARTARRLGIATVAVYSDADRKALHVRV
                     ADTALRIEGYLDIAAVIAAARESGAQAIHPGYGFLAENAEFATACRAAGITFIGPAPE
                     AIAAMGDKAAAKRLMEKAGVPLVPGYHGDRQDDTVLEKEAARIGFPVLIKPCAGGGGK
                     GMKIVRDRSSFAGAVQASKREASSAFGDDRVLIERYLERPRHIEVQVFGDTHGNVIHL
                     FERDCSVQRRHQKVVEEAPAPNFPRKQEIYAAAVAAAKAISYTGAGTVEFIAEQDGRF
                     YFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGERLPQKEPSFRGHAIEARIYAEDP
                     ERDFLPAMGKILHLTFPQQTPSIRIDTGIESGMEITPWYDPMIAKLVVYGSTRASALR
                     RLQQALAEVEIAGVTTNVGFLRRLAASRSLAAAELDTGLIERNRKELLEENPVSMELI
                     AAAAFAELAEEERAARQRASASGDPYSPWHEVDGWRLNEDSHHDLAFIEEAKTHSVRL
                     LFLEAGLRIMVNGCTRELSGERLEDGRLLIRLDGRVFKARAVPEGDDWHVFCQGDYRR
                     LSLKQELAGAGDEASNGSLSAPMPGRVIKVLTAAGAKVTRGQTLLILEAMKMEHTIVA
                     PADGVVKEVHYGAGEQVLEGAELITLA"
     gene            complement(5715..>6144)
                     /locus_tag="VFR57_11585"
     CDS             complement(5715..>6144)
                     /locus_tag="VFR57_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0260042.1"
                     /translation="IAVGTPEAKFCLSEAKLGLSPATISPYVIRAMSERTARRYFLTA
                     EVFDAHEALRIGMLSILTSKEELDGVISSLVEHLLAGGPEAHGSIKTLIRSVAGRRPD
                     DALVAETAQRIAEIRGSPEGREGIAAFLEKRKASWCSPKS"
BASE COUNT         1048 a         2003 c         2010 g         1083 t
ORIGIN      
        1 agcttccgcg acgtcaagtc ctatcggcgc cgcaagcggt ggctttgaag aacgaattct
       61 tcgacaagct ggaaacgcgt gcctcgaagc ggcgcgaggc ggcgctgatg gccgagctgc
      121 cgaggctcgt cgcgcacgcg aggaagcgcg cgtcggggtt tgcgcggatt ttgtctgaag
      181 tgaacccttc cattgttcgc tccagggcgg cgctggcgaa gctccccgtc acacggaaaa
      241 gcgatctcgc cacgctgcag aaggagctgc cgccgctcgg cggcctgaac gcgacgccgg
      301 tagagaagct tgggaagctc ttcatctccc ccgggccgat ttacgacccc gaaggtcgcg
      361 ggaaggactg gtggcgtacc gcgcgcgggc tattcgcagg cggcttccgc gccggggacc
      421 gcgtgctgaa cacgttcgcg taccacttca cccccgccgg ctcgatgctc gaatcgggcg
      481 ccaccgcgat cggctgcacc gttatcccga gcggggtcgg gcagaccgag atgcaggtgg
      541 cagccatccg cgacctgaag gtcaatgcgt tcgtgggcac gccctcgttc ctgaagctca
      601 tagtggagaa ggcagatgag ctgaaggccg acatctcgag cctcgagaaa gcccacgtgg
      661 gggctgagta cttgccccct tcattgagat cttccctgcg tgaccgcggc attcgcgttt
      721 cgcaaaccta tgcaagcgcg gacctcggcc ttatcgctta cgagtcggtg atgccggacg
      781 gcacggtgaa cgaaggcatg atcctcgacg agcgtttgat cctcgagatc gtgcgccccg
      841 gcaccggcga ccccgtcgcc acgggcgagg tcggagaagt cgtgatcacg accttcaata
      901 aggactaccc cctcatccgc ttcgccaccg gcgacctttc ggctgtcctg cccggcacga
      961 gcccctgcgg gcgcacgaac gtgcgtatca agggctggat gggccgcgcc gaccagagca
     1021 ccaaggtgcg cggcatgttc gtcacgcccc gccaggtcaa cgacatcgtg cggcggcacc
     1081 ccgagatagt gcgcgcgcgc ctggtggtcg aaggagaagc aggcgacgac cgcatgacgc
     1141 tcaagtgcga gatgcgtgag cggctccagg gcatcgccga agcgatcgtc gcctcgatcc
     1201 gcgaggtgac caagctgcgc ggcgacgtcc agctcgtcga gcccgggagc ctgccgaacg
     1261 acggtaaggt catcgaggac acccggaaat acggctgacc cgcggctatt tcaccgcgcc
     1321 gcgccgacgg gcaggtcgtc gagccggcgc atctcgtcgc tgctcagcag cgaacggtcc
     1381 tttaggaacg ccttgacaaa gtcgactgcg tcggcgcccc agaacacttc cccgtccacc
     1441 acgaagctcg gcacgccaaa caccccgcgc gcaactgcgt gctccgtatt tctcctgaga
     1501 agggatttga tctgttcatc ggcgagcgcc gccggatcga cgttgagctc tcggcaaagc
     1561 gcctggaaac gtgcgggatc acctgcattc cctccgctca tccaaagcga gtcgaaaatg
     1621 cgtttgacgg cctcgggccg cgattcgcag gctaatgcga ggcgcaggtg ctggagcgga
     1681 ttgaatgggt gctcggccgg gaagcggaac gggatgccga gctcggccgc ccaccaggtg
     1741 caccagcggt atgtccattt gcgctttgcc gagatctcgg ccggtccctt ctggccccag
     1801 tgttgcaaca gaccggcgaa gagcaccggc ttgaaatcga tgtccgcatc cagttccggc
     1861 agccggtgca aacagatata agagtagggc gaaacgaaat cgaagtacca gtcaatggag
     1921 gttgccatgg cggtcaggat agagaagaag ggcaaagtct ggacggtgat ccactcgcgg
     1981 cccgaggcgc gcaatgcgat ggacccggaa agcgccgacg ctctggtttc tgcttttcag
     2041 agctttgata aaaatcccga tgcggcggtc gcggtgttgt ggggggacgg cggtgcgttc
     2101 tgcgcgggat gggacctcaa gtacggcgca tcgctgatga aagacccgac cccgattcac
     2161 gagattgact atccgctcga tgaccgaaag ccgatccccc gagggccgct cggcccgacg
     2221 cgcctcgagc tcgacaagcc ggtgatcgcc gcagtggcgg gcccggcggt ggcgggcggc
     2281 ttcgagctcg cgttgtggtg cgacgtgcgc gtgatggagc agtcggcgta cttcggcgtt
     2341 tattgccgcc gctggggcgt gccgctcatc gacggcggta cggtacgtct gccgcgcatt
     2401 gtcggcatgg gccgtgcact cgagatcatt cttaccgggc gcaaggtccc ggcggaggag
     2461 gcgctgcaca tcggcgcttg cgagcacgtc gttcccgacg gcaagtcgcg cgaattcgcc
     2521 gaagccatgg cgcacgagat cgctcgcttc ccgcaggcct gcacgcgtgc cgaccggcgc
     2581 tcggcctacg cgcagcaagg cttgagcctc agagaagcaa tgcgcaagga gtggtacaac
     2641 ggcattcctg ctttcaaggc ggaaggcgcc gccggcgccg cgcgcttctc gtcaggcaag
     2701 gggcggcacg gcgatttcgg caatatctag cttttttctt ctcgccggca actccctcat
     2761 gaccgcggtc tgctctgtct cggtcatggc accccagcgc gcaatctcat cgagcgtgcg
     2821 gcagcatccg atgcacaccc cgcgcaccgg gtccatctgg cagaccttga tgcatggggt
     2881 tttcatttct tcttcttggc ggaaatggcc aatgctgcct tggaggcggg tttcctgttg
     2941 agcaagccgc agatccactg gccgatctcg accagctggt cgagatcaac gcccgtccgc
     3001 acccccaggc cgttcagcat gtagatgacg tcctcggtgg cgacgttgcc cgacgcgccc
     3061 gcggcatagg ggcagccgcc gagacccgcg accgaggcat cgaaggtcgc gacgcccatt
     3121 tcgagtgagg cgtagatgtt ggcaagcgcc tggccatagg tatcgtggta gtggccggcg
     3181 agccgctcga gcggcacgcg ccgcgcgcag gcctcgatca tcgccttcgt cttgcccggt
     3241 gtgccggtgc cgatggtgtc gccgagcgag acctcgtagc agcccatgtc gtagagcgcg
     3301 ccggcgacct cggcgacctt gtccggcttg atttcgccct cgtacgggca gccgagcacg
     3361 cacgagacat agccgcgcac ccggatgttg gactttcttg attcgagagc tatgggctcg
     3421 aagcgttcca gcgattcggc gatggagcaa ttgatgttct tcttcgagaa cgcctccgat
     3481 gccgcgccga agatcgcgac gtcggtcgcg ccggcggcgc gcgccgcctc gaagcccttg
     3541 aggttcggcg tcaggaccgg gtaggagacg cccggcctgc gccggatgcg ctccataacc
     3601 tcggtgtggt cggccatctg cgggatccac ttgggcgaaa cgaacgcagt cgcttccacc
     3661 gccggaagcc cagccccagc cagccgttcg atgagctcga cctttacgtc agtggaaacc
     3721 tgcccgggct cgttctgcag cccgtccctg ggacccactt ccactatctt tatggcgtca
     3781 agcaagggtg atcagctccg cgccttcgag cacctgctcg cccgcgccgt agtggacttc
     3841 cttcaccacg ccgtcggcgg gcgcgacgat tgtgtgctcc atcttcatcg cctcgagtat
     3901 caggagcgtc tggccgcgcg tcaccttcgc gccggcggcg gtcaatacct tgatgaccct
     3961 gccgggcatg ggtgcggaaa gcgagccgtt gctcgcctcg tcgccggctc cggcgagttc
     4021 ctgcttgagc gaaagccgcc gatagtcgcc ctggcagaag acgtgccagt cgtcgccttc
     4081 gggaaccgcg cgcgccttga atacgcggcc atcgaggcga atgagcaggc ggccgtcttc
     4141 cagccgctcg ccggagagct cgcgggtgca gccattcacc atgatgcgca acccagcctc
     4201 gaggaaaagc agccgcacgg agtgagtctt cgcttcctct atgaaggcta aatcgtggtg
     4261 cgagtcttcg ttcaggcgcc agccatcgac ctcgtgccaa ggggagtacg ggtcgccgga
     4321 agccgatgcg cgctggcgcg ccgcacgctc ttcctcggcc agttccgcga acgcggcagc
     4381 cgcgatcaac tccatgctga cggggttttc ttcgagtaat tctttcctgt tgcgctcgat
     4441 aagaccggta tcgagctctg cggcagccag agaccggctc gcagcaaggc ggcgcagaaa
     4501 tccgacgttg gtggtaaccc cggcaatctc gacctccgcg agtgcctgct ggaggcgccg
     4561 cagcgccgag gctcgagtcg agccgtacac cacaagcttg gcgatcatcg ggtcgtacca
     4621 cggcgtgatc tccatcccgc tttcgattcc ggtatcgatc cggatcgagg gtgtctgttg
     4681 tggaaaagtc agatgcagga tctttcccat cgcgggcagg aagtcgcgct cgggatcctc
     4741 ggcatagatg cgcgcctcga tggcgtgccc cctgaaagaa ggctcctttt ggggtagacg
     4801 ctcccccgcc gcaacgcgca gctgccattc gacgaggtcg aggccggtga tcatctcggt
     4861 aaccgggtgc tcgacctgaa gccgggtatt catctccatg aagtagaagc ggccgtcctg
     4921 ctcggcgatg aactccaccg tgccggcgcc ggtgtacgag atcgcctttg ccgcagccac
     4981 cgccgcggcg tagatctcct gcttgcgcgg aaaattcggc gccggggctt cctcgacgac
     5041 cttctgatgt cggcgctgca ccgagcagtc gcgctcgaag agatggatga cattgccgtg
     5101 ggtgtcgccg aagacctgca cctcgatatg gcgcggccgc tccagatacc gctcgatcag
     5161 cacccggtcg tcgccgaacg ccgagctcgc ttcgcgtttg gacgcctgca ccgcgccggc
     5221 gaaggaagac cggtcccgca cgattttcat ccctttgccg ccgccgccgg cgcagggctt
     5281 gatcagcacc ggaaagccga tgcgcgcggc ctccttctcg aggactgtgt cgtcctgccg
     5341 gtcgccgtgg tagcccggga cgaggggcac accggccttt tccatcaggc gcttcgcggc
     5401 cgccttgtcc cccatcgctg cgatcgcttc gggcgcaggc ccgatgaagg taatgccggc
     5461 cgcgcggcac gcggtggcga actcggcgtt ctccgcaagg aagccgtagc cgggatggat
     5521 cgcctgtgcg ccggattcgc gcgcggcggc gatcaccgcg gcgatatcga ggtagccttc
     5581 gatgcgcagc gccgtgtcgg ccacgcgcac gtgaagcgcc ttccggtcgg cgtccgagta
     5641 caccgccacc gtggcgatac cgagccggcg cgccgtgcgt gcgacccggc atgcgatctc
     5701 cccgcgattg gcgatcagga ttttggcgaa caccaggacg ccttgcgctt ctccaggaat
     5761 gcagcgatgc cttcccggcc ctcgggcgag ccgcggatct cggcaatgcg ctgcgcagtt
     5821 tcggcgacga gcgcgtcgtc ggggcggcgg ccggcaaccg agcggatgag cgtcttgata
     5881 ctgccgtgcg cctccggccc gccggcaagg aggtgctcga caagagaaga aatgaccccg
     5941 tccagctctt cttttgacgt caggattgaa agcatcccga ttcgcagggc ttcgtgcgcg
     6001 tcgaacactt cggccgtgag gaaatagcgc cgggccgtgc gctcactcat ggcgcggatg
     6061 acgtacgggc tgatcgtcgc gggcgagagc ccgagcttcg cctccgagag gcagaacttg
     6121 gcttccggcg tgccgacggc gatg
//
LOCUS       DASTPD010000342         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_595776, whole genome shotgun sequence.
ACCESSION   DASTPD010000342 DASTPD010000000
VERSION     DASTPD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595776"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..905)
                     /locus_tag="VFR57_11590"
     CDS             complement(102..905)
                     /locus_tag="VFR57_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0260043.1"
                     /translation="MNLDYVHGYDRRANVRLQDQADALVDLLHSDTGYPDGASVLEAG
                     CGVGAQTVTLARRSPGARIVSVDIAAASLAAAKAKVDAAGLGNVRFEQADIFALPFER
                     ASFDHVFVCFVLEHLSRPLEALERLKAVLKPGGTMTVIEGDHGSTYFHPESDAAHDAI
                     RCQVELQRAAGGNANIGRELYPLMLRAGLRKVRVSPRMVYVDSSRPHLVDGFTRKTFT
                     AMIEGVRENAIRAGMISPERFDEGIRALYRTAEADGVFCYTFFKGVGEL"
     gene            complement(902..1321)
                     /locus_tag="VFR57_11595"
     CDS             complement(902..1321)
                     /locus_tag="VFR57_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260044.1"
                     /translation="MIILGGCHCGNIRFALTWEPDPVEIPARACTCSFCAKHGGVWTS
                     NPRGKLEVAIEDQERVSNYAFGTRTAEFHICSRCGIVPVVTCRIDGRLYAVVSVNAFE
                     GIAESLLRRAPASFDREDTATRLARRKRNWIGDVTFR"
     gene            complement(1418..2053)
                     /locus_tag="VFR57_11600"
     CDS             complement(1418..2053)
                     /locus_tag="VFR57_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260045.1"
                     /translation="MRRTAGWLLVSLATLWGCSNTVLVPVPPRMELKEYGTLGVAEFS
                     SNYTPAVNARAMRELQARIHAAQPGTRLVELGSREVLLATVGSRQLDPEALKKIGAKY
                     GVDAIFLGEITYSEPKTNININSTSKLEGSARTDVRGDISARLVETRTGAAVWSSSAW
                     ATRQVGRVDVSGYQGVSTTVRNSDARDDMVPTLVSHLTEDFRPSTVRQKVK"
     gene            complement(2080..2688)
                     /locus_tag="VFR57_11605"
     CDS             complement(2080..2688)
                     /locus_tag="VFR57_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0260046.1"
                     /translation="MKLYITPGSPYARMARIVVWEKGLEQRVETIVARTRVSDSPYYA
                     VNPSGRVPYLLCDDGVAFEESAVVCAYLDHLDGKPAFHLPRDGDSWEALRLEALARSM
                     LDGLSVWGREILRPGNERSPGVIRHETDRARRMADVWEREIDHPLMRGALNMIQITLA
                     CALGLEGRNPGFRWRPGRAKLVEWFDRIAERPSFTATAPPSV"
     gene            complement(2685..3392)
                     /locus_tag="VFR57_11610"
     CDS             complement(2685..3392)
                     /locus_tag="VFR57_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0260047.1"
                     /translation="MEKQGIVKREIKALAFDQYGTIVDMQKGLTEAVTPFLKKKGWDG
                     EPSSFVTWWRRTHFENSMIDALCDRGHTPYRQIGQRAVSYVMDRCRIAYTQDEVRWLV
                     GEIEKLKPLSDVVAALQRLRAAGYKLAILSNGDRDMLKAAGPHIGFKFDHVISVQEAG
                     YFKPHWKTYAKAEEIIGEDRSSILFVANHAFDCIGAKSYGMRTAFIDRRKRPFGETPH
                     QPDLVVADLAELAATLA"
     gene            complement(3737..4183)
                     /locus_tag="VFR57_11615"
     CDS             complement(3737..4183)
                     /locus_tag="VFR57_11615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0260048.1"
                     /translation="MTRALLAFLACPGIVAIAIPFIWLAVTSGRQLAHPLGLVPLALG
                     FVALLWCVRDFYVSGRGTLAPWAPPRKLVVVGLYRYTRNPMYVAVTIVLFGWAWAFGS
                     LGLFLYATVVAIAFHLRVVLGEEPWLARAFGSDWQQYAGRVPRWFW"
     gene            complement(4180..>4620)
                     /locus_tag="VFR57_11620"
     CDS             complement(4180..>4620)
                     /locus_tag="VFR57_11620"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0260049.1"
                     /translation="TKNVPAVAVLHEGLKFQVTGPRGEKMLTDMPPPMGGTGSGVNPG
                     WLLRAGLASCNATVIAMRAAQLGVQLKSLEVIVESESDNRGLLGLDDSVSAGLRNLRT
                     KVKIAAEAATPAQLREIVAWAEAHSPVGCTIRQAPKVTTEVEIV"
BASE COUNT          823 a         1523 c         1462 g          812 t
ORIGIN      
        1 cttcgagatc tgcagctcgg tgatgcggaa gccggggcgc gcggcatgct cggcgcggcg
       61 ctcgacttcg tacagcggac ggtcgtcctt cacggcctcg atcatagctc gcctaccccc
      121 ttgaaaaacg tgtagcagaa aacgccgtcc gcttcggcgg tccggtagag cgcgcgaatc
      181 ccttcatcga agcgctcggg cgagatcatg ccagcccgga tcgcgttctc gcgcacgccc
      241 tcgatcatcg cggtgaaggt cttgcgcgtg aagccgtcga cgaggtgcgg acggctcgag
      301 tcgacgtaca ccatgcgcgg cgagacgcgc accttgcgca gccccgcccg cagcatcagc
      361 gggtagagct cgcggccgat gttggcgttg ccgccggcgg cgcgctgaag ctcgacctgg
      421 cagcggatcg cgtcgtgagc ggcatcgctc tcggggtgga agtaggtcga gccgtggtcg
      481 ccctcgatga cggtcatcgt tccacctggc ttcagcactg ctttcagccg ctcgagcgcc
      541 tcgagcggcc gggacaggtg ctcgagcacg aagcagacaa acacatggtc gaaggatgcg
      601 cgctcgaagg gcagggcgaa gatatcggcc tgctcgaagc gcacgttgcc gaggccggcc
      661 gcgtcgacct tggctttagc tgcggcgagc gatgcggctg caatgtccac cgagacgatg
      721 cgcgcgcccg ggctgcggcg cgcgagcgtc acggtctgcg cgccgacgcc gcagcccgcc
      781 tcgagcaccg aggctccgtc ggggtagccg gtgtccgagt gcagcaggtc caccagcgcg
      841 tcggcctggt cctgcagacg aacgttggcg cgccggtcgt agccgtggac gtagtcgaga
      901 ttcaccggaa ggtgacgtcg ccaatccagt tgcgcttgcg ccgtgcgagt cgggtcgccg
      961 tatcctcgcg atcgaagctt gccggcgcgc gtcgcaggag cgattcggcg atcccttcga
     1021 atgcattgac gctaaccacg gcgtagaggc gtccatcgat gcggcatgta accaccggca
     1081 ctatgccgca gcgcgagcag atgtggaatt cggcggttcg cgtgccgaac gcgtagttgc
     1141 ttacgcgctc ttggtcctcg atagccactt ccagcttgcc gcgcggattg gacgtccaca
     1201 ccccgccgtg cttggcgcag aacgagcagg tgcaggcgcg tgccgggatc tcgaccggat
     1261 cgggctccca ggtgagcgcg aaccggatgt tcccgcagtg gcagccgcca aggatgatca
     1321 tcgcgcccct gagagcatga acgcgattat cgccaatgaa aggggtcagg accctttccc
     1381 aacaaaaggg tcctgacccc ttttccccct ttttccgcta tttcaccttc tggcgcaccg
     1441 tgcttggccg gaagtcttcg gtgaggtgcg aaacaagtgt cggcaccatg tcgtcgcgcg
     1501 catccgaatt gcgcactgtc gtgcttacgc cctggtagcc ggagacgtcg actcgcccta
     1561 cctgcctggt cgcccaggcc gagctgctcc acaccgccgc gccggtgcga gtttccacca
     1621 gcctcgccga gatgtcgccc cggacgtcgg tgcgcgcgct gccctcaagc ttggacgtgc
     1681 tgttgatatt gatgttggtc ttcggctcgg agtacgtgat ctcgccgagg aagatggcgt
     1741 ccacgccgta cttggcgccg atctttttca gtgcttccgg atcgagctgg cggctgccga
     1801 ccgtggcaag caagacctcg cggctgccca gctccaccag gcgcgtcccc ggctgtgccg
     1861 catggatgcg cgcctgaagc tcgcgcatcg cgcgtgcgtt gacagcgggg gtgtagttcg
     1921 aagaaaactc ggcgacgccg agcgtcccgt actccttcag ctccatgcgg ggcggcaccg
     1981 gcacgagcac cgtgttggag catccccaca gcgtagcaag cgacaccagc agccaaccgg
     2041 cggtcctgcg cataggacaa cctccctttg ccgcaaagcc tacacgctgg gaggcgcagt
     2101 cgccgtgaac gatggccgct cggcgatgcg gtcgaaccat tccacgagct tcgcgcgccc
     2161 gggtcgccag cggaagcccg ggttgcgccc ctcgaggccg agagcgcatg cgagcgtgat
     2221 ctggatcatg ttgagcgcgc cgcgcatcag cggatggtcg atttcacgct cccaaacgtc
     2281 ggccatgcgt cgggcacgat cggtttcatg gcgaatcaca cccggcgagc gttcgttccc
     2341 gggccgaagg atctcgcgtc cccagaccga aaggccgtcg agcatgctcc tcgccagcgc
     2401 ctcgaggcgc agcgcctccc acgagtcgcc atcccgcggc aggtggaacg ccggtttgcc
     2461 gtccaggtga tcgaggtaag cgcagacgac agcggattcc tcgaacgcaa caccgtcgtc
     2521 gcacagcaga tagggcactc gccccgatgg attgacggcg tagtagggac tatccgaaac
     2581 gcgtgtcctg gcgacgatcg tctcgacgcg ctgctccagt cccttctccc aaacaacgat
     2641 ccgcgccatg cgcgcataag gagagccggg ggtgatgtac agcttcacgc gagcgtcgcg
     2701 gccagctcgg caagatcggc cacgacgaga tcgggctggt gcggcgtctc tccgaacggg
     2761 cgcttgcgcc ggtcgatgaa tgccgtgcgc atgccgtagg actttgcgcc gatgcagtcg
     2821 aatgcatggt tggcgacgaa gaggatgctc gaacgatcct cgccgatgat ttcctcggcc
     2881 ttcgcgtaag tcttccagtg cggcttgaaa tagcccgctt cctggaccga gatcacgtga
     2941 tcgaacttga agccgatgtg cggcccggcg gccttcagca tgtcgcggtc gccgttcgag
     3001 aggatggcga gcttgtatcc cgcggcgcgc agcctctgca gcgccgcgac aacatcggaa
     3061 aggggcttca gcttctcgat ttcgcctaca agccagcgca cctcgtcctg cgtataggcg
     3121 atgcggcagc gatccatgac gtaggaaacc gcgcgctgcc cgatctgccg gtagggcgta
     3181 tggccgcgat cgcaaagcgc atcgatcatc gagttttcga aatgcgttcg ccgccaccag
     3241 gtgacgaagc tgctcggctc gccgtcccag cctttcttct tcagaaaggg cgtcaccgcc
     3301 tcggtgaggc ccttctgcat gtcgacgatg gtcccgtact ggtcgaacgc cagcgccttg
     3361 atctcgcgct tgacgatgcc ctgcttctcc atcaaagctc gctcagccgc cttgctctgc
     3421 cttggacgcc gaagcaagcg tcgagatgct ccgctgcctc gaacaccgcc tggtctcggt
     3481 gcggccgcag tggcgagcac tccaggtgca cgcggcagcc ttctcgcaaa acgcccgcat
     3541 agaatccctc gtggttaaat tcgacctcga agccgagccg ggctcgatag tactcgagcg
     3601 agcgggatta gtcggcaact ctgaataccg gcgcgacgta atgcaggctg cgtcgagatt
     3661 tactcatagg tttccctcgg agacgttacc cctgccgcac gcggtccagc gcggctgggc
     3721 tgattccggg gcagcgctac cagaaccagc gcggcacccg cccggcatat tgttgccagt
     3781 cgctgccgaa cgcgcgagcg agccatggct cttcgccgag cacgactctg agatgaaacg
     3841 ctatggcgac gaccgttgcg tagaggaaca acccgagcga gccgaacgcc cacgcccagc
     3901 cgaacaggac aatcgtcacc gcaacgtaca tcggatttcg cgtgtagcga taaagaccga
     3961 ccacgaccag cttgcgcgga ggcgcccatg gcgcgagcgt acccctgccc gaaacgtaga
     4021 agtcgcgcac gcaccagagc agcgcgacga acccgagcgc gagcggcacc aggcccaacg
     4081 ggtgcgcgag ctgcctgccc gacgtgaccg cgagccagat gaaggggatg gcgatcgcga
     4141 cgatgcctgg gcatgcgaga aacgcgagca acgcgcgtgt caaacgattt ccacttcagt
     4201 tgtcaccttg ggcgcttggc ggattgtgca gccgactggg gaatgcgctt ctgcccatgc
     4261 cacgatttct cgtagttgcg ccggcgtagc cgcttccgca gcgatcttca ccttcgtgcg
     4321 taggttgcgc agtccggcgg acacgctgtc atccaagccc agcaatcccc gattgtcgga
     4381 ctcactctcg acgatgacct cgagggactt gagctgcaca cccaactggg ctgccctcat
     4441 cgcgataaca gtcgcgttgc aggaagccaa ccccgctctt agcaaccagc cgggattgac
     4501 gcccgaaccc gtgccgccca tcgggggagg catatcggtg agcatctttt caccacgagg
     4561 tccggtaact tgaaacttca gaccttcgtg gagcactgcc acagcaggta cattcttcgt
//
LOCUS       DASTPD010000343         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_802563, whole genome shotgun sequence.
ACCESSION   DASTPD010000343 DASTPD010000000
VERSION     DASTPD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802563"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1242
                     /locus_tag="VFR57_11625"
     CDS             88..1242
                     /locus_tag="VFR57_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0260050.1"
                     /translation="MRRLYLRIYLAVLASLAVFAIAAGALWHILADPHGGEEVGALVA
                     RNLLPPAGAPPAEQQAALERLARDVRADLALFSRDGARLGSVGAPLPAPERGGWQRGP
                     GGPHWATQLPDGRWLAARTPHQRRGPAFGLLAFALILVAIGVGAYPVVRRLTKRLERL
                     QHGVEALGAGELSARVKVEGRDEVAKLAESFNRAAARIEELVAAHKTLLANASHELRT
                     PLARIRMALELGKDKRAVEQDIAELDALIDEVLLASRLDAVSSLENIEDVDLLALAAE
                     EASRYDVEAQGEPTMMRGDPRLLRRMIRNLLENARRHGAPPIGVSVRDREIRVCDSGP
                     GVAEADRERIFERFNSKHGTGLGLALVRQIARRHGGEARCEGNCFVVGFK"
     gene            complement(1346..2137)
                     /locus_tag="VFR57_11630"
     CDS             complement(1346..2137)
                     /locus_tag="VFR57_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0260051.1"
                     /translation="MALYRGMDQVALDAAYNNSAAVKDSAQIVAEWEARSARLRARHA
                     EGMNLRYGPEERNRIDYFAARRDGPVLAFIHGGYWQMRAKELFAFIAEGPLAWGINVA
                     LIGYTLAPQKRLDGIVAEIHAALDWLNSSIPTLGGDRERVFISGWSAGGHLTAMAMSH
                     PAARGGLAISGIYDLEPMRLCYINDKLRLDEDEARRNTPGHTEKPLVIAYGADELPEL
                     CRQSEEYAKLLRREAIAVPERNHFTILEELASPHGALTALARRLG"
     gene            complement(2137..3123)
                     /locus_tag="VFR57_11635"
     CDS             complement(2137..3123)
                     /locus_tag="VFR57_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260052.1"
                     /translation="MIRLTLACWDYDRTRALMDGTVSPDGIELIYLNQPVEETFFRMM
                     RYREFDCSEMSLSSYVASLNTQHPPFIAIPAFPSRFFRHSCIFVSAKSGIRKPEELQG
                     KRIGVPEYQMTAPVWIRGILSDDYGVKVTDVEHFSGGEEEPGRDEKLKLDLPSNIRLR
                     PIPGDKTLSRMLAEGELDALVTARAPSTFHKEPDKVKRLFPNYVEVEKEYYRRTRIFP
                     PMHTVVMRRDVYAKHPWVAQSLYKAFVEAKAKAYALYNQTAALPAMVPWLVAEIEEAR
                     RDMGEDWWPYGLEPNRKALETFLRYHHEQGLSKRRFQPEELFARETLASFKV"
     gene            complement(3120..4241)
                     /locus_tag="VFR57_11640"
     CDS             complement(3120..4241)
                     /locus_tag="VFR57_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0260053.1"
                     /translation="MKTLKIGVAGLGRAFALMAPAFRDPRVVLVAAADPREEARNKFS
                     QDFEARAYGTVEELCSHPAVDIVYVATPHEFHAAHAQLACSRRKHVLVEKPMALSLAD
                     CRAMVNAARENAVQLVVGHSHSFDLPVLRTKELIQSGAYGEVRMITALDFTDWVYRPR
                     RPEELTAAVQNQAAHQVDITRLLAGGRVCSVRATTGSFDPKRPAEAAWSCQLRFDNGV
                     FASLIYSGYAHFDSDEFMGWIGEMGLKKDPSVYGEARRALTAEEVSLKNARNYGGPQF
                     RDTLPSVAHHHFGLFIVSCEKGDLRPMPNGVMIYGDQKKHLEEVPTPTIPRAEVIDEL
                     YAAVVEGRAPLHDGEWAMATLQVCLALLDSARSGKEVLL"
     gene            4257..5534
                     /locus_tag="VFR57_11645"
     CDS             4257..5534
                     /locus_tag="VFR57_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0260054.1"
                     /translation="MLTKEQNELLCRVEGAAPMGGIMRRHWLPVCMSEEAAEADGKPV
                     RSRLLGEDLVVFRNSKNQIGVLSEYCLHRRASLAFGRNEECGLRCLYHGWKFDVEGNV
                     VDMPSEAPGAAQRLGKKTKAYPVREGGGFVWVWMGPKEEIREFEPPAWAPKPDIRYAI
                     VKMHVACNWAQVLEGSIDSAHSSTLHSTNMPAAADVYGSTATESAWLRPSNDKAPRLQ
                     FEATDYGFRYAAIRKPIKNPERDQYVRTTLFIAPFTVLIPPNNQYNLAQMLVPIDDVN
                     TMFYWIAWHADPAKGITQQAWRRFCAAEVGVDLDENFRKRRTLENRYLQDREAMKHGD
                     WTGIVGIPAQDMAMWESMGPITDRSEDHFGSSDLAVAQFRRMMVAAAEKFQEGAPAIG
                     TRKSVRRADLASFEGIVPKSTDWRTLGNTYCLA"
BASE COUNT          958 a         1750 c         1888 g          988 t
ORIGIN      
        1 gaagagcagt atctggagcg aagattcggt gcccaatact tggattacaa ggcttccgtg
       61 cgccgctggc tttaaggtgg tgggctgatg cgcaggctgt acctgcggat ctatctcgcg
      121 gtgctggcga gcctcgcggt attcgcgatc gccgcgggcg cgctctggca catcctcgcc
      181 gacccgcatg gcggggagga ggtcggcgca ctcgtcgcac gcaacctgct gccgccggct
      241 ggcgctcccc cggccgagca gcaggcggcg ctcgagcgcc tcgcgcgcga cgtgcgcgcc
      301 gacctcgcgc tcttctcgcg tgacggagcg cgcctgggat cggtcggcgc gccgctgccc
      361 gcgcccgagc gcggcggctg gcaacgcgga ccgggcggcc cccattgggc gacacagcta
      421 cccgacgggc gctggctggc ggcgcgaacg ccgcaccagc ggcgcgggcc ggcgttcggc
      481 cttctggcgt tcgcgctcat cctcgtcgcc atcggcgtcg gcgcctatcc ggtggtgcgc
      541 cgtctcacca agcgcctcga gcggctgcag cacggcgtgg aggcgctcgg cgcgggcgag
      601 ctctcggcgc gcgtgaaggt cgaggggcgg gacgaggtgg cgaagcttgc cgagagcttc
      661 aaccgcgcgg cggcgcgcat cgaggagctg gtggccgcgc acaagacgct gctcgccaac
      721 gcgtcgcacg agctgcgcac gccgcttgcc cgcatccgca tggcgctcga gctaggcaag
      781 gacaagcgcg ccgtcgagca ggacatcgcc gagctcgatg cgctgatcga tgaggtcctg
      841 ctcgcgagcc gcctcgacgc ggtgtcttcg ctggaaaaca tcgaggacgt cgatctgctc
      901 gcgctcgccg ctgaggaggc gtcgcgctac gacgtcgagg cacagggcga gccgacgatg
      961 atgcgcggcg atccacggct gcttcgccgc atgatccgca acctcctcga gaacgcacgc
     1021 cgccacggtg cgccgccgat cggggtgtcg gtgagagatc gcgagatccg cgtgtgcgat
     1081 tccgggcccg gcgttgcgga ggccgatcgg gagcggatct tcgagcgttt caactcgaag
     1141 cacggcaccg gcctcggcct cgcgctcgtg cgccagattg cgcgccgcca tggcggcgag
     1201 gctcgttgcg aaggaaactg ctttgtcgtg gggttcaaat gaaagagagt gaaacagaag
     1261 atctttgcga cccgagtgac cgcctcccga tcgcgctgct cgtgctgtcg ctcgccctcg
     1321 tgctcggctg ggtgtttttc ggcggctagc ccagccttcg cgcgagcgcg gtcagcgcgc
     1381 cgtgcggcga ggcaagttct tccaatatgg tgaagtgatt cctttcagga acggcaatcg
     1441 cttctcgcct tagaagtttc gcgtactcct cggactgccg gcagagctcc ggcaattcgt
     1501 ctgcaccgta ggcaatgaca agcggttttt cggtatggcc cggggtgttt cgccgagcct
     1561 cgtcctcatc gagccggagc ttgtcgttga tgtagcaaag ccgcatcggc tcgagatcgt
     1621 agatgccgct gatggcaagg ccgccccggg ccgcgggatg gctcatcgcc attgcggtga
     1681 gatgcccgcc ggcggaccag cccgaaatga atacccgctc tctatccccg cccagggttg
     1741 gtatggacga gttcaaccag tcgagcgcgg catggatctc ggcgacgatg ccgtcgaggc
     1801 gcttctgcgg agcgagcgtg tagccgataa gcgcgacgtt gatgccccag gcgagcgggc
     1861 cttcggcgat gaaggcgaaa agctccttgg cgcgcatctg ccaatagccg ccgtggatga
     1921 aggcgagtac cggcccgtcg cgccgcgcgg cgaaatagtc gatacggttg cgctcctcgg
     1981 gcccgtagcg caggttcatg ccctcggcat gcctggcgcg cagccgcgcg ctgcgcgctt
     2041 cccactcagc gacgatctgc gcgctgtcct tgaccgcggc gctgttgttg taggcggcgt
     2101 cgagcgccac ctggtccatc cctcgataga gagccatcag actttgaatg acgcgagcgt
     2161 ttcccgtgca aaaagctctt cgggctggaa gcggcgcttc gacaagccct gttcgtggtg
     2221 gtagcgcagg aaggtctcga gcgccttgcg gttcggctcc aggccgtacg gccaccagtc
     2281 ctcgcccatg tctcgccgcg cctcctcgat ttcggcgacg agccatggga ccatggcggg
     2341 cagggcggcg gtctggttgt agagcgcgta cgccttcgcc ttggcttcga cgaatgcctt
     2401 gtagagcgat tgcgcgaccc aggggtgctt cgcgtagacg tcgcggcgca tcacgaccgt
     2461 gtgcatcggc ggaaagatcc tcgtgcgccg gtagtactcc ttctccactt ccacgtagtt
     2521 cgggaataga cgtttgacct tgtcgggctc cttgtggaac gtcgaaggcg ctctggcggt
     2581 caccaatgca tcaagttcgc cttcggcgag catccgtgac agggttttgt cgcccggaat
     2641 cggcctcagt cttatgttcg aaggcaaatc gagcttcagt ttttcatcgc ggccgggctc
     2701 ttcctcgccg ccggaaaaat gctcgacgtc agtgactttc acaccgtagt cgtccgacag
     2761 gatgccgcgg atccagaccg gcgccgtcat ctggtactcg ggcacgccga tgcgcttgcc
     2821 ctgaagctcc tccggtttgc ggatgccgct cttcgccgag acgaagatgc atgagtggcg
     2881 gaagaagcgc gacgggaatg ccggaatggc gatgaaggga ggatgctggg tgttcagcga
     2941 agcgacgtat gacgaaagcg acatctccga gcagtcgaat tcccggtagc gcatcatccg
     3001 gaaaaacgtc tcttcgaccg gctggttcag gtagatgagc tcgatgccgt cgggcgagac
     3061 cgtaccgtcc atgagcgccc gcgtgcggtc gtagtcccag caggcaagcg tgaggcgtat
     3121 caaagcagca cttcctttcc gctgcgggcg gaatcgagaa gcgccaagca tacctgcagg
     3181 gtcgccatcg cccattcgcc gtcgtggagc ggcgcacgcc cttccaccac ggcggcgtac
     3241 agttcgtcga tcacttccgc acgcgggatg gtaggggttg gcacctcctc caggtgcttc
     3301 ttctggtcgc cgtagatcat cacgccgttg ggcatcgggc gcaggtcgcc cttttcgcag
     3361 gagacgatga acaggccgaa atggtggtgc gcgacggagg gaagcgtgtc cctgaactgc
     3421 ggccctccgt agttccgggc attctttaag gaaacctcct cggcggtaag cgcgcgccga
     3481 gcttctccat agacggacgg atccttcttc agccccattt cgccgatcca gcccatgaat
     3541 tcatccgagt cgaaatgggc gtagccgctg tagatgaggc ttgcgaacac gccgttgtcg
     3601 aagcgaagct gacacgacca cgccgcctcg gctgggcgct tcgggtcgaa gctgccggtg
     3661 gtggcgcgca cgctgcagac ccttccgccg gcgagcaggc gcgtgatatc cacctgatgc
     3721 gccgcctggt tctgcaccgc ggcggtgagc tcttcgggcc ggcgcgggcg atagacccaa
     3781 tcggtgaagt cgagcgcggt gatcatccgc acttcgccgt aggcgccgct ttgaatcagc
     3841 tctttcgtcc ttagaaccgg caagtcaaag ctatggctgt gcccgaccac gagttgaacg
     3901 gcgttctccc tggcggcgtt caccatcgca cggcagtccg cgagcgaaag tgccatcggc
     3961 ttttcgacaa ggacatgctt cctgcgcgag caggccaact gggcatgcgc ggcgtggaat
     4021 tcgtgcggcg ttgccacata gacaatgtcc accgccggat gggagcaaag ctcttccacg
     4081 gttccataag cccgagcttc gaagtcctga gagaacttgt ttcgggcttc ctcgcgaggg
     4141 tccgcggcgg cgacgagcac cacgcgtggg tcgcggaatg ccggcgccat gagcgcgaat
     4201 gcacggccca ggccggccac gccgattttc agggttttca tgttatgtat atgggcatgt
     4261 taacgaagga gcagaacgag cttctctgcc gggtcgaagg cgcggcgccg atgggcggca
     4321 tcatgcgccg ccactggctc ccggtgtgca tgtccgagga agcggccgag gccgatggca
     4381 agccggtgcg ctcgaggctc cttggcgaag acttggtggt tttcaggaac tcaaagaacc
     4441 agattggtgt tctgagcgaa tactgcctgc accgccgggc ctcgctggcg ttcgggcgca
     4501 acgaggaatg cggcctgcgc tgcctgtacc acggctggaa gttcgacgtc gagggcaacg
     4561 tcgtcgacat gccttcggaa gcaccgggcg cggcgcagcg cctgggcaag aagaccaagg
     4621 cgtacccggt gcgcgagggc ggcggctttg tgtgggtgtg gatgggcccg aaggaggaga
     4681 tccgggagtt cgagccgccc gcctgggcgc ccaagccgga catcaggtac gcgatcgtga
     4741 agatgcacgt tgcgtgcaac tgggcgcagg tactggaagg ctccatcgac tcggcgcata
     4801 gctcgacgct gcattccacc aacatgccgg cagcggcgga tgtttacggc tcgaccgcga
     4861 ccgagtcggc atggctgcgg ccctcgaacg acaaggcacc gcgcctgcag ttcgaagcca
     4921 ccgactacgg cttccgctac gcggcgatcc gcaagccgat caagaatccg gagcgagacc
     4981 agtacgtacg cacgacgctc ttcatcgccc cctttacagt gctcatcccg ccgaacaacc
     5041 agtacaacct cgcgcagatg ctcgtgccga tcgacgacgt caacacgatg ttctactgga
     5101 tcgcctggca cgccgacccg gcgaagggca tcacccagca ggcgtggcgg cgcttctgcg
     5161 ccgctgaagt gggagtcgac ctggacgaaa acttcaggaa gaggagaact ctggagaatc
     5221 gttacttgca ggaccgcgaa gcgatgaagc acggcgactg gaccgggatc gtgggcatcc
     5281 cggcgcagga catggcgatg tgggagtcga tgggaccgat cacggaccgg tccgaggatc
     5341 acttcggctc gagcgacctc gcggtggcgc agttccgccg catgatggtc gcggcggcgg
     5401 agaaattcca ggaaggcgcg ccggcgatcg gcacgcggaa aagcgtccgc cgcgcagatc
     5461 ttgcgtcgtt cgagggcatc gtgccgaaga gcacggactg gcgaacgctg ggaaatactt
     5521 actgtttggc gtaggagggc ggcgccgcag ccctggggcg ccagcacgcc gaaggtgaag
     5581 taac
//
LOCUS       DASTPD010000344         7290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1618123, whole genome shotgun sequence.
ACCESSION   DASTPD010000344 DASTPD010000000
VERSION     DASTPD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618123"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFR57_11650"
     CDS             <1..216
                     /locus_tag="VFR57_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0260055.1"
                     /translation="HTGECELIGDKLGGIAVHIGARVAGKAEPGEIVVSQTVRDLVAG
                     SGLQFAERGVHSLKGVPGEWRLYAFAG"
     gene            complement(264..1448)
                     /locus_tag="VFR57_11655"
     CDS             complement(264..1448)
                     /locus_tag="VFR57_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0260056.1"
                     /translation="MNRAVGASKIEHYVSDEPFEPHAVETLTPEQERFYRASQWQIMW
                     WKFRRHRIAVVAGIILLAFYASTLVSEFIAPYDLHTRDTNHIYAPPQRVHLFDQGRFV
                     GPFVYGYAMRLNMETLKREYVVDLNTVYRLRFFCLGDNYEFWSLIPGRFHFVCPAENG
                     TLYLLGTDRLGRDLFSRIVYGTRISLTVGLFGIAVSFLIGITLGGISGYYGGWVDNAI
                     QRFIEMVRSFPELPLWMALSAALPINWSPILVYFGITIILGLLDWPGLARAVRSKLLA
                     LREEDFATAAVLMGASPARVIRKHLLPSFTSHLIASATLSVPAMILGETALSFLGLGL
                     RPPVTSWGVLLNEAQNINVVALYPWLMLSVVPVFIVVLAFNFFGDGLRDAADPYSSVA
                     RT"
     gene            complement(1445..2470)
                     /locus_tag="VFR57_11660"
     CDS             complement(1445..2470)
                     /locus_tag="VFR57_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0260057.1"
                     /translation="MLRYTLRRMLIMIPTLIVISFVTFVIIQLPPGDFLSNQIAELQS
                     QGDKAAMEKAEFLRKQYGLDKPFLEQYAIWIGVWPGENGFSGLIQGDLGWSFEHDLPV
                     KEVVGDRLLLSFILNFSVILFTWAVAFPIGFYSATRQYSWGDHGLTFLGYVGLATPNF
                     LFALVLMYFANVQFGLSVGGIMAPEYLGQPWSIGKARSVLEHLWIPVIVIGTSGTAGM
                     IRRLRANLLDELQKPYVVTARAKGMPPFGLLVKYPLRMALNPFIADIGNLLPSVISGS
                     AIVAVVLSLQTSGPMLLEALRSQDQYLAGSFLMFLSLLTVIGMFISDLLLAALDPRIR
                     LTGGVAK"
     gene            complement(2470..4329)
                     /locus_tag="VFR57_11665"
     CDS             complement(2470..4329)
                     /locus_tag="VFR57_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260058.1"
                     /translation="MRFLFPLLFVPALALGQNFVEPPMLRLDVSTGRLPPTEKRLPQP
                     PLVVRMDEPGKHGGALHSLVGRSRDTRLLVVYGYARLVGYDRNLELAPDILESFEVQE
                     GRIFTLRLRKGHRWSDGHPFTAEDFRYYWEDVANNKELSPAGPPRDLLVDGEPPKFEI
                     LSDTVLRYTWHKPNPHFLPRQAGASPLFIYRPAHYLKPLHKKYSEKVRKAEADGTAKR
                     RWSAVHNRADNLYESDNPDLPTLQPWMNTTRPPADRFVAVRNPYFHRVDERGQQLPYI
                     DRFVLAVADPKLIPAKTGAGEADLQARDVHFNNYTFLKQGEKQNGYRTLLWRPGKGSH
                     FALFPNLNTKDPVWRQLLRDVRFRRALSLGIDRSLVNQVLYFGLAIESNNTVLPQSPL
                     FREAYRDRWARYDRKAAIQLLEEIGLKKGRDGVRRLPDGRPLEIIVETAGESTEHSDV
                     LELIRETWREVGIKVFTKPSQREAWRNRIYSGETVMSVWGGIENGLATPDTSPDELAP
                     MSQTQLQWPKFGQYFETGGKSGEPVDMPEVKELQRLYELWMSATSRREREQAWHAMLA
                     LHSEQQFTIGVVSGVPQPVVARDTLMNVPERGFYNWDPGAFFGIYRPDTFWFR"
     gene            complement(4326..6161)
                     /locus_tag="VFR57_11670"
     CDS             complement(4326..6161)
                     /locus_tag="VFR57_11670"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0260059.1"
                     /translation="MADLLTVRDLKVYLDVDAGTVKAVDGVSFRIPAGRTVALVGESG
                     SGKSVVAQSIMSILPRIARIESGEIILRDPEAKREVDISRLDPAGRTMQDIRGGRISI
                     IFQEPMTALSPLHTIGNQIGEALALHRPEIDKAGRKAAVIEMLRRVGFPDPVKAEHTY
                     PFELSGGLRQRAMIAMALVCRPALLIADEPTTALDVTIQAQILQLIQNLQAEFDMAVL
                     LITHDLGVVANMADEVVVLHRGRVMESGTVSDIFRDARHPYLRALLRAVPRFNMAPGE
                     RLTPIREVQVSADSLITAEKRNASSGTLLQVRNLTKRYTIRKRSFLGAQSAGELLAVD
                     DVSFDVQAGECVGLVGESGCGKTTTARMILRSVTPDAGTIHFKDRDVLALKGEQLFEY
                     RNQVQFVFQDPFSSLNPRMTAYDIVSEPLVIHGVGDADERFARVKELMGLVGLDVRHL
                     RRYPHSFSGGQRQRIGIARALALGPELLICDEPVSALDVSVQAQVLNLLVDLRKALGL
                     AYLFISHNLAVVNYVADRIAVMCAGRIVELAPSAELFRNPVHPYTRALLAAVPAPDLG
                     RKLDFDALMEGKASDPAAWPAEFRIAEGLRPSMQDIGRGHLVRAA"
     gene            complement(6154..6861)
                     /locus_tag="VFR57_11675"
     CDS             complement(6154..6861)
                     /locus_tag="VFR57_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260060.1"
                     /translation="MSWEEFEAEIARRGGVKFWWRDDDAAAASPELRRALALSRTCAV
                     PLALAVIPEAAEPALFGLLHERITLLQHGTDHRNRAAPGEKKTEYPAAEPIEAALGRV
                     SDGFRRLKTFAAERLLPVLAPPWNRVRKDLLDSLPAVGIRGVTLYGPRMGALIPQVNT
                     HVDIVAWRRGRRFIGEDEALRLAAKNLSDEPLGWLTHHAVHDEDAWRFLERLFKVQGV
                     HWLSAAEAFSYTGLKNG"
     gene            complement(6858..>7290)
                     /locus_tag="VFR57_11680"
     CDS             complement(6858..>7290)
                     /locus_tag="VFR57_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0260061.1"
                     /translation="IAARPLTRLHDRPWRLLAGVNASVARTRDGVIVERSRDDFSLRL
                     RNCLVSVSQAGYNTVMETLQAGARAVLVPFAAGIEVEQTIRAQLLAERGLVHVVEESV
                     LTPATLAAAIDGAVLAPRPAPQRIDLGGARRTAELMREWLK"
BASE COUNT         1331 a         2280 c         2483 g         1196 t
ORIGIN      
        1 cacaccggtg aatgcgagct cattggcgac aagctgggcg ggattgcggt ccacatcggc
       61 gcgcgcgtcg ccggcaaggc cgagcccggc gagatcgtcg tgtcgcagac cgtgagggat
      121 ctcgtcgccg gttcgggcct ccagttcgcc gagcgcggcg tgcattctct gaagggcgtg
      181 ccgggcgaat ggcgtttgta cgcgtttgcc ggctagccga gccaagcacc cccgaatcgg
      241 aaatcgggct ctgaccccga ttttcaggtt cgggcgacgg agctgtaggg gtcggcggcg
      301 tcgcgcaggc cgtcgccgaa gaagttgaag gcgaggacga cgatgaagac tggaaccacc
      361 gaaagcatga gccaggggta gagcgcgacg acgttgatgt tctgcgcttc gttgagaagc
      421 acgccccagc tggtcaccgg cggtctcaaa cccaaaccca gaaacgatag cgcggtctcg
      481 ccgaggatca tggcgggcac cgacagcgtt gccgaggcga tgaggtggct cgtaaaggac
      541 ggaagcaggt gcttgcggat gacgcgcgcc gggcttgcgc ccatgaggac cgcagcggtt
      601 gcgaaatcct cctctctcaa tgccaacaac ttggagcgca cggcgcgagc cagacccggc
      661 cagtcgagaa ggccgaggat gatggtgatg ccgaagtaaa cgaggatcgg actccagttg
      721 atcggtagag ctgccgaaag cgccatccac agcggcaatt cggggaatga gcgcaccatt
      781 tcgatgaagc gctggatcgc gttgtcgacc cagccgccgt aatagccgga aatgccgccg
      841 agcgtgatgc cgatgaggaa gctcaccgcg atgccgaaga ggcccacggt gagcgaaatg
      901 cgcgtgccgt agacgatgcg ggaaaagagg tcgcggccga ggcggtcggt tccgaggaga
      961 tagagcgtgc cgttctccgc ggggcagacg aaatgaaatc gcccggggat gaggctccag
     1021 aactcgtagt tgtcgccgag gcagaagaag cggaggcgat agaccgtgtt caggtcgacg
     1081 acgtattcgc gcttcagcgt ctccatgttg aggcgcatcg cgtagccata gacgaacggc
     1141 ccgacgaagc ggccctgatc gaagaggtgc acccgctgcg gtggtgcgta gatatggttg
     1201 gtgtcgcgcg tgtgcaggtc gtacggcgcg atgaattcgc tgaccagcgt ggacgcgtaa
     1261 aaggcgagaa gaatgatgcc ggcgacgacc gcaatgcggt ggcggcggaa tttccaccac
     1321 atgatctgcc attgagacgc gcggtagaag cgctcttgct ccggcgtgag cgtttcaacg
     1381 gcgtgcggct cgaagggctc gtccgaaaca tagtgttcga tcttcgaagc cccaaccgcc
     1441 cggttcattt ggccacgcca ccggtgaggc ggatgcgcgg gtcgagcgcg gcgagcagca
     1501 gatcggagat gaacatcccg atgacggtga ggagagacag gaacatcaga aacgaaccgg
     1561 cgaggtactg gtcctggctg cgcagcgctt cgagcagcat cgggcccgag gtttgcagcg
     1621 acagcaccac cgcgacgatc gcggagccgg agatcacgct cggcagaagg ttgccgatgt
     1681 cggcgatgaa cgggttgagc gccatgcgca gcgggtactt cacgagcagg ccgaagggcg
     1741 gcatgccctt cgcacgcgcg gtcaccacgt acggcttctg cagctcgtcg agcaggttgg
     1801 cgcgcaggcg ccggatcatg ccggcagtgc ccgacgtgcc gatcacgatc accgggatcc
     1861 acaggtgctc gagcaccgag cgcgccttgc cgatgctcca cggctgtccg aggtactccg
     1921 gggccatgat gccgcccacc gaaaggccga actggacgtt cgcgaagtac atgagcacga
     1981 gcgcgaagag gaagttcggc gtggcgaggc cgacgtagcc gaggaaggta aggccgtggt
     2041 cgccccagct gtactggcgc gtcgccgagt agaaaccgat cgggaaggcg accgcccagg
     2101 tgaaaaggat cacggagaaa ttcaggatga aggaaagcaa gaggcgatcg ccgaccacct
     2161 ccttcaccgg cagatcatgt tcgaacgacc agccgaggtc gccttggatg aggcccgaga
     2221 agccgttttc ccccggccac acgccgatcc agatcgcgta ctgctcgagg aacggcttgt
     2281 cgagcccgta ctgcttgcgc aggaactcgg ctttctccat cgctgccttg tcgccctggc
     2341 tttgcagctc ggcgatctgg ttggagagaa agtcgcccgg cggcagctgg atgatcacga
     2401 aggtgacgaa gctgatcacg atcagcgtcg ggatcatgat caacatccgc cgcagcgtgt
     2461 aacgcagcat taccggaacc agaacgtgtc gggtcggtag attccgaaga acgcccccgg
     2521 gtcccagttg tagaacccgc gctctggaac gttcatgagc gtgtcgcgcg ccaccaccgg
     2581 ttgcggtacg ccggagacaa cgccgatggt gaactgctgc tccgagtgca acgcgagcat
     2641 cgcgtgccac gcctgctcgc gctcgcggcg cgacgttgcg ctcatccaga gctcgtaaag
     2701 ccgctgcagc tccttgactt ccggcatatc gaccggctct ccggatttgc cgccggtctc
     2761 gaaatactgt ccgaacttcg gccattggag ctgcgtctgg ctcatcggcg cgagctcgtc
     2821 ggggctcgta tccggcgtgg cgagtccgtt ctcgatcccg ccccacaccg acattaccgt
     2881 ctcgccggaa tagatgcggt tgcgccacgc ttcgcgctgc gacggcttgg tgaacacctt
     2941 gatgccaacc tcgcgccatg tctctcggat gagctcgaga acgtcgctgt gctcggtcga
     3001 ttctccggcg gtctcgacga tgatttcgag cggccggccg tcgggcaggc ggcgaacgcc
     3061 gtcccttccc ttcttgagtc cgatctcctc gaggagctgg atggccgcct tgcggtcata
     3121 gcgcgcccag cggtcgcgat acgcctcgcg gaacagcggg ctctgcggca gcacggtgtt
     3181 gttcgactcg atcgcgaggc cgaaatagag cacctggttc acgagcgagc ggtcgatgcc
     3241 cagggaaagc gctcgccgga agcgcacgtc gcgcagaagc tgccgccaca ccggatcttt
     3301 ggtgttgagg ttcgggaaaa gcgcgaagtg cgaacccttg cccggccgcc agaggagcgt
     3361 gcggtagccg ttctgttttt cgccctgctt gagaaacgtg taattgttga aatgcacgtc
     3421 gcgcgcctgc aggtccgctt ccccggcgcc ggtcttcgcc ggaatgagct tggggtcggc
     3481 cacggcgagc acgaagcggt cgatataggg gagctgctgg ccgcgctcgt ccacccgatg
     3541 gaagtacgga ttgcgcaccg cgacgaagcg gtcggcgggc gggcgcgtgg tattcatcca
     3601 cggctgcagc gtcggcagat ccgggttgtc cgattcgtaa aggttgtcgg cacggttgtg
     3661 caccgccgac cagcgccgct tcgccgtgcc gtcggcctcg gccttgcgca ctttctcgga
     3721 gtacttcttg tggagcggct tcaaataatg cgccgggcgg tagatgaaaa gcggcgaagc
     3781 gccggcttgg cgcggcagga aatgcgggtt gggcttgtgc caggtatagc gcagaacggt
     3841 atcggacagg atttcgaact tcggcggctc gccgtccacc aggaggtcgc gcggcggccc
     3901 agccggcgag agctccttgt tattcgcgac gtcctcccag taatagcgga agtcttcagc
     3961 ggtaaacgga tgtccatccg accagcgatg acccttgcgc aaccgtaggg tgaaaatgcg
     4021 cccttcctgg acttcgaacg attccaggat gtcgggagcg agctccaggt tgcggtcgta
     4081 gccgacgagg cgcgcatagc cgtaaacaac cagtaggcgg gtatcgcggc tgcgtcctac
     4141 gaggctgtgc agggcgccac cgtgcttgcc cggctcgtcc atgcgcacga cgagcggcgg
     4201 ctggggcagc cgtttttcgg tcggcggcag ccgtcccgtg ctcacgtcga gccgcagcat
     4261 cggcggctcg acgaagttct ggccgagcgc cagggcgggc acgaagagca gtgggaagag
     4321 gaacctcatg cggctcgcac caggtggccc cgtccgatgt cctgcatcga tggccggagg
     4381 ccctcggcga tgcggaattc cgctggccat gccgcggggt cggaagcctt gccttccatc
     4441 agcgcgtcga agtcgagctt gcggccgaga tcgggcgcgg gtaccgcggc aagcagcgct
     4501 cgcgtgtacg gatgaaccgg atttcggaag agctccgccg acggcgccag ctcgacgatg
     4561 cgcccggcgc acatcaccgc gatccggtcg gcgacgtagt tcaccacggc caggttgtgc
     4621 gaaatgaaga ggtaggcgag gccaagcgcc ttgcgcagat cgacgagcag gttcagcacc
     4681 tgagcctgca cggagacgtc gagcgccgaa accggctcat cgcagatgag gagctcgggg
     4741 ccaagggcga gcgctcgcgc gatgccgatc ctctggcgct ggccgccgga gaagctgtgc
     4801 ggatagcgcc gcaggtggcg cacgtccagg ccgaccaggc ccatcagctc cttgacgcgc
     4861 gcgaagcgct cgtccgcgtc gccaacgccg tggatgacga gcggctcgct cacgatgtcg
     4921 tatgccgtca tgcgcgggtt gagcgagctg aacggatcct ggaacacaaa ctgcacctgg
     4981 tttcggtatt cgaaaagctg ctcgcccttg agtgcgagca cgtccctgtc cttgaaatgg
     5041 attgtgccgg cgtcgggcgt cacggagcgc aggatcatgc gcgccgtggt cgtcttgccg
     5101 cagcccgact caccgacgag gcccacgcac tcgcctgcct gcacgtcgaa gctcacgtcg
     5161 tccaccgcca ggagctcgcc ggcgctctgc gctccgagga aagaccgttt gcggatggtg
     5221 tagcgcttcg tgaggttgcg gacttggagg agcgtgcccg aggacgcgtt tcgtttttcc
     5281 gccgtgatca ggctgtcggc gctgacttgc acctcgcgga tcggcgtgag gcgctcgccg
     5341 ggggccatgt tgaaacgcgg caccgcgcgc agcaatgcgc gcagatacgg gtggcgcgca
     5401 tcgcggaaga tgtccgagac ggttccggac tccatgacgc ggccgcggtg caggaccacg
     5461 acctcatcgg ccatgttcgc caccacgcca aggtcgtgcg tgatcaagag cacggccatg
     5521 tcaaattcgg cctgcaggtt ctggatgagc tggaggatct gcgcctggat ggtcacgtcg
     5581 agcgcggtgg tcggctcgtc ggcaatgagg agagccggac ggcagacgag cgccatcgca
     5641 atcatcgcgc gctgccgcag cccgccggag agctcgaagg gatacgtgtg ctcggccttc
     5701 accggatccg gaaagccgac gcggcgcagc atctcgatca ccgctgcttt gcgcccggcc
     5761 ttgtcgatct cgggacgatg gagcgcgagc gcctcgccga tctggttgcc gatcgtgtgc
     5821 agcggcgaca gcgccgtcat cggctcctgg aagatgatcg agatgcgtcc cccgcgaatg
     5881 tcctgcatgg tgcggcccgc gggatcgagc cgtgagatgt ccacttcgcg cttcgcctcc
     5941 ggatcgcgca ggatgatctc gccggactcg atgcgcgcga tgcgcggcag gatgctcatg
     6001 atcgactggg ccacaaccga cttgcccgag cccgactcgc ccacgagcgc gacggtccgc
     6061 cccgcgggaa tgcggaacga aacgccgtcg accgccttca cggtccccgc atcgacgtca
     6121 agatagacct taaggtcccg gaccgtcagc agatcagcca ttcttgagac cagtatacga
     6181 gaaggcttcg gctgccgaga gccagtgcac gccctggacc ttgaaaagcc gctcgaggaa
     6241 tcgccaggcg tcctcatcgt gcaccgcatg gtgcgtaagc cagccgagcg gctcgtccga
     6301 aaggttcttc gcggcgagcc gcagcgcctc atcctcaccg atgaatcgcc gcccacgccg
     6361 ccaagccacg atgtcgacat gggtgttgac ctgcggaatg agcgcgccca tgcgcgggcc
     6421 gtacagggtc acgccgcgga tgccgaccgc cggcaggctg tccagcaggt ccttgcgcac
     6481 gcggttccac ggcggcgcga gaacgggcaa gagacgctcc gcggcgaagg tcttcaagcg
     6541 cctgaagccg tcggacaccc gcccgagcgc cgcctcgatc ggctcggccg ctggatactc
     6601 ggtcttcttc tcaccagggg cggcgcgatt gcggtggtcg gtgccatgct gcagcagcgt
     6661 gatgcgttcg tgcaagagcc cgaaaagcgc aggctcggcc gcttccggta tcacggcgag
     6721 cgcgagcgga actgcgcagg tgcgcgaaag cgcgagtgcc cgtcttagct cgggtgaagc
     6781 ggcggcggca tcgtcatcgc gccaccagaa cttcacgccg ccgcgccgcg cgatctcggc
     6841 ttcgaattct tcccaactca cttgagccac tcgcgcatca gctccgccgt gcgccgggcg
     6901 ccgccgagat cgatgcgctg cggcgccggg cgcggcgcga gcacggcgcc atcgatcgcg
     6961 gcggcgagcg tcgccggcgt gagcacgctc tcctccacga cgtgcacgag gccacgttcc
     7021 gcaagcagct gggcgcgaat ggtttgctcc acctcaatcc ccgcagcgaa cggcacgagc
     7081 accgcgcgcg cgcctgcctg caaggtctcc atgacggtgt tgtaacccgc ctggctcacg
     7141 gataccaagc agttcctgag gcgcagggaa aaatcatcgc gcgaacgttc tacgatcacg
     7201 ccgtcgcgag tacgagctac ggaagcgttg acgccggcga gcaggcgcca cggtcggtca
     7261 tggagccggg tgagcggccg cgcggcgatc
//
LOCUS       DASTPD010000345         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_598214, whole genome shotgun sequence.
ACCESSION   DASTPD010000345 DASTPD010000000
VERSION     DASTPD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598214"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFR57_11685"
     CDS             complement(<1..478)
                     /locus_tag="VFR57_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260062.1"
                     /translation="MSTATRFATVALGLAILALSAWAQAPIPRIGFLGNSTPALEANL
                     VGPFRDGLRDLGYVEGKTIAIEYRWAEGDYTRFPTLIKELIAAKVDIIVTAGTPATLA
                     VKKATRSVPVVMIAVGDPVGTGIVSSLRLPGGNITGLTSISPELDGKRLELLREVVP"
     gene            complement(475..1029)
                     /gene="slmA"
                     /locus_tag="VFR57_11690"
     CDS             complement(475..1029)
                     /gene="slmA"
                     /locus_tag="VFR57_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEU0260063.1"
                     /translation="MPRAKGERRLEILKALAQMLEAPRWEKITTAALAERLGVSEAAL
                     YRHFASKAQMYEGLIEFIESSVFTLANQITADEADGHSQCEKLVEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARMNQFFERFESTMKQSLRVASDGDAPGRAGALLRYALGCLH
                     QYAKSGFAKKPSESFSVQRRYLLA"
     gene            complement(1049..1705)
                     /locus_tag="VFR57_11695"
     CDS             complement(1049..1705)
                     /locus_tag="VFR57_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEU0260064.1"
                     /translation="MRIARLPPSRRVWIFDLDNTLHDARVHIFPSMHTQIQNYLKRQF
                     GLNDEDAGRMRREFWLRYGTTLAGLMRHHGTDPSHFLAETHVFPELADMVVRENALKH
                     ALARLGGTRLVFSNAPRHYVEQVLRAIRLRRFFDAVYTIEDTGYRGKPHAWGFHRLLR
                     NHDLDPHRCALIDDILDNLRAAHRLGMSTVWVSPVGRRAPYVDLRIASVTELPRRIHS
                     "
     gene            complement(1708..>2308)
                     /gene="argB"
                     /locus_tag="VFR57_11700"
     CDS             complement(1708..>2308)
                     /gene="argB"
                     /locus_tag="VFR57_11700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0260065.1"
                     /translation="ETMDVVEMVLGGQVNKEVVELINQAGGKAVGLTGQDGAFIHARK
                     MTLPSKENGRVDLGQVGEIDSIDPAVIEALEAGGFIPVVAPIGTGRDGTTYNINADLV
                     AGKLAEVLRAEKLVVLTNTPGVLDKDGKLLTGLTPKKIDELVAKGVISGGMLPKIGSA
                     LDAARSGVKSVHIIDGRVPHALLLEVLTDQGVGTLIRSK"
BASE COUNT          372 a          748 c          772 g          416 t
ORIGIN      
        1 ccggcacgac ttcgcgcaga agctctaggc gcttgccgtc gagctcgggc gagatcgagg
       61 tcaagccggt gatgttgcct ccgggcaggc gcagcgagga aacgatgccg gtacccaccg
      121 gatcacccac cgcgatcatg acgaccggaa ccgacctggt cgccttcttg accgcgagcg
      181 tggccggagt tcccgcggtg acaatgatgt cgaccttggc ggcgataagc tctttgatga
      241 gcgtcggaaa gcgcgtgtaa tcgccctcgg cccagcggta ctcgatcgca atggtcttgc
      301 cctcgacgta cccgaggtcg cgcaggccgt cgcgaaacgg tcccacgagg ttcgcttcga
      361 gcgccggggt cgagttgccg aggaacccga tgcggggaat cggtgcctgc gcccaggcag
      421 agagcgcgag gatggcaagg ccaagcgcta cggtcgcgaa gcgcgtcgcg gtgctcaagc
      481 tagtagatag cgccgctgga cgctgaagct ctcgctcggc ttcttggcga agccgctctt
      541 tgcgtattga tgcaggcagc cgagcgcgta gcgaaggagc gccccggcgc ggccgggggc
      601 gtcgccgtcg gaggcgacgc gcagcgactg cttcatcgtc gactcgaagc gctcgaagaa
      661 ctggttcatg cgcgcttgca ggcgctcgtg ctcgcccacg agcgcatcgc cggtcatgac
      721 gcgcaccatg ccggggttct tctcggcgaa ggcgagcagc atttcgacca gtttctcgca
      781 ctggctgtgg ccgtcggcct cgtccgcggt gatctggttc gcgagcgtga acaccgagct
      841 ttcgatgaac tcgatcagcc cctcgtacat ctgcgccttg gaggcgaagt ggcggtagag
      901 cgcggcttcc gagacgccga gcctttcggc aagcgcggcg gtagtgatct tctcccacct
      961 cggcgcctcg agcatctgcg caagcgcttt caggatctcg agccggcgtt cgcctttggc
     1021 gcggggcaca gtagaagaat tttaagcttc aggagtggat tctacgcggc agttcggtga
     1081 ctgaggcaat gcgcaggtcg acgtaaggcg cgcgccgccc caccgggctc acccaaaccg
     1141 ttgacatgcc aaggcgatgt gccgcgcgca agttgtccaa gatgtcgtcg atcagggcgc
     1201 agcgatgagg gtcgaggtcg tggttgcgaa gcagccggtg aaagccccac gcgtgcggct
     1261 ttccgcggta gcccgtgtcc tcgatcgtgt agaccgcgtc gaagaagcgg cgtagccgaa
     1321 tcgcgcgcag cacctgctcc acgtagtgtc gtggcgcatt tgaaaacacg agccgcgtgc
     1381 cgcccagccg cgcgagcgcg tgcttgagcg cattctcgcg cacgaccatg tcggctagct
     1441 cggggaagac gtgcgtctcg gcgaggaagt gactcgggtc ggtgccgtgg tggcgcatga
     1501 ggccggcgag cgtagtgcca tagcgcagcc agaactcgcg ccgcatccgg ccggcgtctt
     1561 cgtcattcag cccgaactgc ctcttcaggt aattctggat ctgcgtgtgc atcgagggaa
     1621 agatgtgcac ccgcgcgtcg tgcagcgtgt tgtcgaggtc gaagatccac acccgccgcg
     1681 agggcggcaa cctcgcgatc ctcactgtta cttgcttcgg atcagcgtgc cgacgccctg
     1741 gtcggtgagc acttcgagaa gcagcgcgtg cggcacgcgc ccgtcgatga tgtgcacgct
     1801 cttcacgccg ctcctcgcgg cgtccagcgc cgagccgatc ttgggcagca tgccgccgga
     1861 aatcacgcct ttcgcgacca gctcgtcgat cttcttcggt gtgagcccgg tgaggagctt
     1921 gccgtccttg tcgagtacgc cgggcgtatt ggtcagcacg acgagcttct cggcgcgcag
     1981 cacctcggcg agcttgcccg cgaccaggtc ggcgttgatg ttgtaggtgg tgccgtcgcg
     2041 cccggtgccg atcggcgcga ccaccgggat gaagccgccg gcctcgagcg cctcgatcac
     2101 cgcggggtcg atcgagtcga tctcgcccac ctgccccaag tcgacgcgcc cgttctcctt
     2161 ggagggcagc gtcatcttgc gcgcatggat gaaggccccg tcctgcccgg tcaggccgac
     2221 cgccttgccg cccgcctggt tgatgagctc gaccacctcc ttgttcacct ggccgccgag
     2281 caccatctcg accacgtcca tggtctcg
//
LOCUS       DASTPD010000346         9193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1618918, whole genome shotgun sequence.
ACCESSION   DASTPD010000346 DASTPD010000000
VERSION     DASTPD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618918"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VFR57_11705"
     CDS             complement(<1..1279)
                     /locus_tag="VFR57_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0260066.1"
                     /translation="MLPATTDVLIAGGGPCGLVLAIELGRRGIPVVLVDQDAGTTHNA
                     AANATQARTMEHFRRLGFAGEIRALGLPPQFPTDVAYFTRFARHELARFALPSSRDAR
                     QLVTTLSGSWSAAELPHRCNQKFIEPVLRRHAEALPGVSVNYGWRLASFEQFHDRVEA
                     RVQSSSEDETITARYLVGADGARSTVRQALGIAYAGETGVTRHFVGGRMYAVHARIPD
                     FYREVRHRPAWMNVAFNPERRCFMPAVDGRSEFAFHTQLGSNENENSVTDAEAIAMVQ
                     QVIGLPLRVEVISRAAWTAGHSLVAESFSRKRVFIGGDAAHLFTPTGGLGYNTAVEDA
                     VNLGWKIAAVLKGWAAPALLGTYEAERHPLAVRNTGYAKGFADSLGNYVPSSEIEDDT
                     AAGAAARRRASEYFAAHGRAEFNIPGITFGGRYD"
     gene            1519..2472
                     /locus_tag="VFR57_11710"
     CDS             1519..2472
                     /locus_tag="VFR57_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU0260067.1"
                     /translation="MARIRAFENAAEEASRGSVAAFGASLAQSDIRGPLHLSTGQEAV
                     AAGVCINLSSEDFLTSTHRGHGHTLAKGASMDAMMAELFGRASGPNRGKGGSMHLADF
                     SVGMLGANGVVAAGMPIAVGAAHALKLGNRPHVVACFFGDGAVNRGPFLEALNWAAIH
                     ALPILFVCEDNRWSATTQTGSMTAGEGALARARAIGVPGAQVDGNDVYAVDAAAAEFL
                     SEVRKGDGPRFLHAVTYRFKGHVSVDAAGYRDGAEVAKALENDPLVVAARAIPEGEAK
                     RIRNEAAEEVRRALAAAAAAPWPDAADAYRDVQNNGSGQWR"
     gene            2463..3395
                     /locus_tag="VFR57_11715"
     CDS             2463..3395
                     /locus_tag="VFR57_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0260068.1"
                     /translation="MALMSYAQAACAALRQAMKADPTMVVVGEDVGRGGIFQQYNGLQ
                     QEFGAARVIDAPISEATILGAGVGMALAGLKPVVEMRVVDFALCAMDEIVNQAAKARY
                     MFGGQARVPLVARLPIGLWSGSAAQHSQSLEAWFAHVPGLVVVAPATPQDNYSLLSAA
                     IDCGDPVVYMEHKELWGLQGEVSQEKDMLGRARILREGDDITLVTWSRTVHAALEAAG
                     KARQSVEIVDLRTLWPWDRKTVLTSVRKTRKLLVVHEAVQAAGFGAEIAAAVAEELQV
                     PVRRLGAPRIPVGYSKPLEDEARVGAQAILTALS"
     gene            complement(3388..4776)
                     /locus_tag="VFR57_11720"
     CDS             complement(3388..4776)
                     /locus_tag="VFR57_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0260069.1"
                     /translation="MIDWIGTHLALILFLVLTVALFCGFPVAFVLGGIAIFFGALGIL
                     FDVFQPVQFFNLLPRIWGGVAANPILVAVPMFIFMGTVLERSGVANDLLHALQVLTRR
                     IPGGLAMSVTLMGTIMAATTGIVGASVVMLTLVALPAMLARGYQKELTVGTIASAGTL
                     GILIPPSIMLVIMGDLLAVSVGTLFAAAVIPGLVMSALYLTYICMLSAIKPQLAPPLP
                     PDVGPKTRAEFWRMVGKSFFPPTFLIILVLGSIFGGWATPTEAAGVGSAGALFLAWLN
                     GRLSFKDLNGMIESAALTNALVFFIFFGATLFAFVFRALGGDELVVDIMKAVGLDTGW
                     EILVFMMVLVFILGFFFDWIEICLIVLPVFAPALRTVDFTSHIGPGGSIVFLTWFIIM
                     ISVNLQTAFLTPPFGFTLFYMKGTVPPSVTMAHIYRGIMPFVILQLLGLILTIVFPEL
                     ALWLPRAVGFLS"
     gene            complement(4773..5339)
                     /locus_tag="VFR57_11725"
     CDS             complement(4773..5339)
                     /locus_tag="VFR57_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0260070.1"
                     /translation="MERLLAVSKAIERALYRVAMAAGWLFVACTVVIVFDVLTRKFGY
                     QLPGMGSTHLQELEWHLHTALFSFWLGIAYIKNAHVRIDIAIAAAKPRTQALFELAGV
                     LVFALPYCLIALYFSTDFTLIAWRLNEYSDSATGLPWRWIPKGIITVGLVLLLAAVVA
                     VGLRLVVYLWGPQRLRAAAAYAGAKAHP"
     gene            complement(5381..6421)
                     /locus_tag="VFR57_11730"
     CDS             complement(5381..6421)
                     /locus_tag="VFR57_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0260071.1"
                     /translation="MNHGFIAAAAAITLALSAPAQAQQKVRLNLASAFPSSLTLIGEA
                     PVKLVKKIDRLSAGTLEIRFNEPGALVPALQAIQATSQGSVDAAWSNGGFFASTDSAF
                     NMFASVPFGPGIPEYIAWMYHGGGLTLSRELFGKHGIHNIPCAIIPPEASGWFRKEIK
                     TVGDLKGLKMRFFGLGAKTMAKLGVATQLLAPGDIFQALQLGTIDATEFSLPSMDQKF
                     GFYQVAKFYYFPGWHQQATFLELYLNKKRFDSLSDQHKAMLEAACGELLRDVVAEGEA
                     TQWKAMTEMRDKHGVKIMRWSPEIMAAIEKAWNEVIAEESKASANFKRVYDSYAKFRS
                     DYAVWREHGYLK"
     gene            6547..8298
                     /gene="frdA"
                     /locus_tag="VFR57_11735"
     CDS             6547..8298
                     /gene="frdA"
                     /locus_tag="VFR57_11735"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEU0260072.1"
                     /translation="MESVSTDVAIVGAGGAGLRAALALAEAAPKLRIALVSKVYPMRS
                     HTCAAEGGAAGVIKPDDSLQQHFDDTVGGGDWLSDQDAVAYFIAEAPKELLQLERWGC
                     PWSRESDGSVAVRPFGGMKLMRTWFAADKSGFHILHTLFQTSLKYPSIQRFDEYYVTE
                     LLVEDGRCRGLTAIEMRSGQMRRFDAAAVILAGGGAGRMFPFTTNGAIKTGDAMALAY
                     HVGVPLKDMEFVQYHPTGLPSTGTLLTEACRGEGGVLLNKHGRRFLQDYGMGPETPLG
                     KPVLKTMELGPRDRVSQAFWHEQRKGNAVSTRWGDAMLLDMRHLGEKTINERLPLVRD
                     MSISYMGVDPVSDPVPVRPVVHYMMGGIHTDIKAATPVAGLFAAGECACVSINGANRL
                     GSNSLTELLVFGRRAALSALEFLQKGGARPASASLADNAQGKIRALFQRSGGGETVAG
                     LRKEMLNTMEEHAGIYRSGEGLAAACAKLGELRRRYRNLELHDKTNVYNTDLLQALEL
                     GSMLDCAEAVVQSAAARKESRGAHQRLDFPEREDRTYLRHSMATYAGDGPPHIGYRDV
                     VITRSQPGVRDYSGAHA"
     gene            8295..9029
                     /locus_tag="VFR57_11740"
     CDS             8295..9029
                     /locus_tag="VFR57_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU0260073.1"
                     /translation="MRKVELEVLRYNPETDREPHFQRYSVDCQEEWVVLDALNHVKET
                     LDPTLSYRWSCHMAVCGSCGMMINGEPKLACKAFLRNYEGTIRVEPLRHFPVERDLVT
                     VIDDFVAKLARVKPWLMPKEQAPATGEYRQTPAQLKRYKQFTLCINCMLCYAACPEYG
                     LIPKYIGPAAIALAHRWNRDSRDGGRDARQEEIASKEGAWECSFVGACSEVCPEHVDP
                     AGAIQQVKIESTVDWYQAHLMPWLNK"
BASE COUNT         1650 a         2928 c         3168 g         1447 t
ORIGIN      
        1 cgtcatagcg gccgccgaaa gtgatacccg gaatgttgaa ctcggcgcgg ccatgcgcgg
       61 cgaagtattc gctggcgcga cggcgcgccg cggcgccggc ggctgtgtcg tcctcgattt
      121 cggacgatgg aacgtagttg ccgagcgaat cggcgaaccc cttggcgtac ccggtgttgc
      181 ggaccgcgag cgggtggcgc tcggcctcgt aggttccgag cagcgcgggt gcggcccagc
      241 ccttgagcac ggcggcgatc ttccagccga ggttcactgc gtcctcgacc gcggtgttgt
      301 agccgagccc tccggtaggg gtaaagagat gcgcggcgtc cccgccaata aagactctct
      361 ttctggaaaa gctttccgct acgagcgaat gccccgcggt ccacgccgcg cgcgagatga
      421 cctctacccg cagcggcagc ccaatgacct gttggaccat tgcgatcgct tcggcgtccg
      481 tgacggagtt ttcgttttcg ttagaaccaa gttgtgtgtg gaaggcgaac tcgcttctgc
      541 catccaccgc cggcatgaag cagcggcgtt ccggattgaa ggccacgttc atccacgccg
      601 gccggtgccg cacctcgcgg tagaagtccg ggatgcgcgc gtggactgcg tacatgcggc
      661 cgcccacgaa gtggcgtgtg acgccggttt cgcccgcata ggcaatgcca agcgcctggc
      721 gcaccgtgct gcgcgcgccg tccgcgccga ccaggtaacg cgcagtgatg gtttcgtctt
      781 cggaagagct ttgcacgcgc gcctcgacgc ggtcgtgaaa ctgctcgaag gacgccaggc
      841 gccatccgta gttcaccgat acgccgggca gcgcctcggc atgccggcgc agcaccggct
      901 cgatgaactt ctgattgcag cggtgcggga gctccgcggc gctccaggat cccgaaagcg
      961 tagtgacgag ttgccttgcg tcacgcgacg agggcagagc aaagcgcgcg agttcgtgcc
     1021 gcgcgaagcg cgtgaagtag gcgacgtcgg tcgggaactg cggcggcagg ccgagcgcgc
     1081 gaatctcgcc ggcgaagccg aggcgccgga agtgctccat ggtgcgcgcc tgggtggcat
     1141 tcgccgccgc attgtgggtc gttcccgcgt cctggtcgac gagcaccacc gggatgccgc
     1201 ggcgcccgag ctcgatggcg agcacgagcc cgcaggggcc gccgccggca atgagcacat
     1261 cggttgtcgc cgggagcatg tgtggacgcg attttcgcag cccggcgggt cgtccgttgg
     1321 ggatgccgag cccggcgtgc tatcggcgct acgctcggcg atcggcgaag ccccacgaac
     1381 gatggctcga gtataggcgg caagccgtga cagctttgcg accgaggata gtcctcgcgc
     1441 cgcgtcgcgg aagggcgact gtcgtaccat cgcggtcccg tggcttccac cgatcgcaag
     1501 ctctgcctgt atcgcgcgat ggcgcgcatc cgcgctttcg agaacgcggc cgaggaagct
     1561 tcgcgcggca gcgtcgccgc gttcggcgcg agcctcgcgc agtcggatat ccgcgggccg
     1621 ctccacctat caacaggcca ggaggctgtc gcggcgggtg tctgcataaa cctgtcttcc
     1681 gaggacttcc ttacttcaac ccatcgggga catggtcaca cgctggccaa aggcgcgtcg
     1741 atggacgcaa tgatggccga gcttttcggg cgcgcgagcg gtccgaaccg cggcaagggc
     1801 ggctcgatgc acctcgccga cttctcggtc gggatgctcg gcgcgaacgg cgtggtcgcc
     1861 gccggcatgc cgatcgcggt aggcgccgct cacgcattga agctaggaaa tcgtcctcat
     1921 gtagtggcat gttttttcgg cgacggcgcg gtgaaccgtg ggcctttcct tgaggcactg
     1981 aactgggcgg ccatccacgc gctgccgatc cttttcgtct gcgaggacaa ccgctggtcg
     2041 gcgacgacgc agaccggctc catgaccgcg ggcgagggcg cgctcgcgcg cgcgcgcgcg
     2101 attggcgtgc ccggcgcaca ggtcgacgga aacgacgttt acgccgtcga cgccgctgcg
     2161 gcggagtttc tttcagaagt gagaaaaggc gacgggccga ggtttctgca cgcggtgacc
     2221 taccgcttca agggacatgt ctcggtggac gcggcgggtt accgggatgg cgccgaagtg
     2281 gcgaaggccc tcgagaacga cccgctcgtc gttgcggcga gagcgatccc cgaaggcgag
     2341 gcgaaaagga tccgaaacga ggccgcggag gaagtgcggc gcgcgctcgc cgcggccgct
     2401 gcggcgcctt ggcccgatgc ggcggacgcc tatcgcgacg tacagaacaa cgggagcggc
     2461 caatggcgct gatgagttat gcgcaggcgg catgcgctgc gctccggcag gcgatgaaag
     2521 ccgatccaac aatggtggtc gtcggcgagg acgtgggccg cggcgggatt ttccagcagt
     2581 acaacggcct gcagcaggag ttcggcgccg cgcgcgtgat cgacgcacca atctcggagg
     2641 cgaccattct cggcgccggc gtcggcatgg cgctcgccgg tctaaagcct gtggtcgaga
     2701 tgcgcgtcgt ggactttgcg ctctgcgcga tggacgaaat cgtcaaccag gcggccaagg
     2761 cgcgctacat gtttggcgga caggctcggg tgccgctcgt ggcgcggctg ccgatcggac
     2821 tgtggagcgg ctcggcggcg cagcactcac aaagcctgga agcctggttc gcgcacgtgc
     2881 ccggcctcgt ggtggtcgcg ccggcgacgc cgcaggacaa ctattcgctg ctgagcgccg
     2941 cgatcgattg cggcgacccg gtcgtctaca tggagcacaa ggagctttgg ggtctgcaag
     3001 gcgaagtcag ccaggaaaag gacatgcttg gacgtgcccg catccttcgt gaaggagacg
     3061 acatcacgct cgtcacatgg tcgcgaaccg ttcatgcggc gctcgaagct gccgggaagg
     3121 ctcggcagtc cgtagaaatc gtcgatctgc gcacgctttg gccctgggac cgaaaaacgg
     3181 ttctcacatc cgtgaggaag acgagaaagc tgctcgtcgt ccacgaagcg gtgcaggcgg
     3241 cgggattcgg cgccgagatc gccgccgcgg tggccgagga gctgcaggtg cccgtgaggc
     3301 gcctgggagc gccgcggatc ccggtgggct actccaagcc gctggaagac gaagcgcgcg
     3361 tcggcgcaca ggcgatcctc accgcgctca gctgaggaaa ccgaccgcgc gcggcagcca
     3421 gagcgcgagc tccgggaaga cgatcgtgag gatcaggccg agaagctgga ggatcacgaa
     3481 cggcatgatg ccgcggtaga tgtgggccat ggtgaccgaa ggcggcaccg tccccttcat
     3541 gtagaagagc gtgaagccga acggcggcgt aaggaatgcg gtctgcaggt tcaccgagat
     3601 catgatgatg aaccacgtca ggaagacgat cgacccgccg gggccgatgt gcgaggtgaa
     3661 gtccacagtg cgcagcgccg gcgcgaacac cggcagcacg atgaggcaga tctcgatcca
     3721 gtcgaagaag aacccgagga tgaagaccag caccatcatg aagacgagga tctcccagcc
     3781 ggtatcgagc ccgacggctt tcatgatgtc gaccaccagc tcgtcgccgc cgagcgcgcg
     3841 gaacacgaag gcgaacagcg tcgcaccgaa gaagatgaag aacacgagcg cgttggtgag
     3901 cgccgcgctc tcaatcatcc cgttcaggtc cttgaaggac aggcgcccgt tcagccaggc
     3961 gaggaacagc gcgcccgccg agccgacgcc ggcggcttcg gtgggcgtcg cccatccgcc
     4021 gaagatcgag cccaggacca ggatgatgag gaaggtcggc ggaaagaagc tcttgccgac
     4081 catcctccag aactcggccc gcgtcttcgg ccccacgtcg ggcggcagcg gcggcgcgag
     4141 ctgcggcttg atggcgctca gcatgcagat gtaggtgaga tagagcgcag acatcaccag
     4201 cccggggatc acggcggcgg cgaagagcgt gccgaccgac accgcgagca ggtcgcccat
     4261 gatgacgagc atgatcgacg gcgggatcag gatgccgagc gtgcccgccg aggcgatggt
     4321 gccgacggtg agctccttct ggtagccgcg cgcgagcatc gccggaagcg cgaccagcgt
     4381 gagcatgacc accgatgcgc cgacgatgcc ggtggtcgcc gccatgatcg tgcccatgag
     4441 cgtcaccgac atggcgagcc cgcccgggat ccgccgggtc aggacctgca gcgcatgcag
     4501 cagatcgttc gcgactccgc tgcgctcgag caccgtgccc atgaagatga acatcggcac
     4561 cgccaccagg atcgggttgg cggcgacgcc gccccagatg cgcggcagca ggttgaagaa
     4621 ctgcaccggc tggaacacgt cgaagagaat cccgagcgcg ccgaagaaga tcgcgatccc
     4681 gccgagaacg aacgccaccg ggaagccgca gaaaagggcc accgtaagca cgaggaacag
     4741 gatcagcgcg agatgcgtgc cgatccagtc gatcacgggt gcgcctttgc gccggcgtac
     4801 gcggcggccg cgcgcagccg ctgcgggccc cacaggtaga ccacgaggcg aagccccacc
     4861 gcgaccaccg cggcgagcag caggacaagt cccacggtga tgatgccctt cgggatccag
     4921 cgccacggca gcccggtcgc cgagtcggaa tactcgttca gccgccaggc gatcagcgtg
     4981 aagtcggtgc tgaagtaaag cgcgatcagg cagtagggca gcgcgaagac cagcacgccg
     5041 gcgagctcga acagcgcctg ggtgcgcggc tttgccgccg cgatcgcgat gtcgatgcgc
     5101 acatgcgcgt tcttgatgta ggcgatgccg agccagaacg agaacagcgc cgtgtgcagg
     5161 tgccactcga gctcctgcag gtgcgtcgat cccatcccgg ggagctggta gccgaacttg
     5221 cgcgtgagga catcgaacac gatcacgacc gtgcaggcga cgaacagcca gcccgcggcc
     5281 atcgcgacgc ggtacagcgc gcgctcgatc gccttgctta cagcaagaag tctttccact
     5341 gagcgagcaa aaggggccgg agaattctcc ggcccggttt ttacttcaga tagccgtgct
     5401 cccgccaaac cgcgtagtcg gagcggaact tggcgtacga gtcgtagacg cgcttgaagt
     5461 tcgcgctggc tttcgattcc tcggcgatca cctcgttcca cgccttctcg atcgccgcca
     5521 tgatctcggg cgaccagcgc atgatcttca cgccgtgctt gtcgcgcatc tcggtcatgg
     5581 ccttccattg cgtcgcctcg ccctcggcga ccacgtcgcg aaggagctcg ccgcacgcgg
     5641 cttcgagcat cgccttgtgc tggtcggaga gcgagtcgaa gcgcttcttg ttcaggtaaa
     5701 gctcaaggaa ggtggcctgc tggtgccagc cggggaagta atagaacttc gccacctggt
     5761 agaagccgaa cttctggtcc atcgacggca gcgagaactc ggtggcgtcg atggtgccga
     5821 gctgcagcgc ctggaagatg tcgccggggg cgaggagctg cgtcgccacg ccgagcttgg
     5881 ccatggtctt cgcccccagc ccgaagaagc gcatcttcag gcccttgagg tcgcccacgg
     5941 tcttgatttc cttgcggaac cagccggagg cctcgggcgg gatgatggcg cagggaatgt
     6001 tgtggatgcc gtgcttgccg aagagctcgc gcgagagcgt gagtccgccg ccgtgataca
     6061 tccacgcgat gtactcgggt atcccagggc cgaatggcac cgacgcaaac atattgaacg
     6121 cggagtcggt gctcgcgaag aagccgccgt tcgaccacgc ggcgtcgacc gagccctggg
     6181 aggtggcctg gatcgcctga agcgccggca cgagcgcccc gggctcgttg aagcggattt
     6241 cgagcgtccc cgccgagagg cgatcgatct tcttcaccag cttgaccggc gcttcgccga
     6301 tcagggtgag gctggacgga aacgcgctcg cgagattgag cctgactttc tgctgcgcct
     6361 gtgccggcgc cgagagcgcg agcgtaattg ccgcggcggc ggcgatgaaa ccgtggttca
     6421 tgtttctccc cctccgggtg tggatctgac tgccgcatta tccacaatcg gaccggattt
     6481 gatctaggac aacttcggaa cccgccttgg ttgcacattg gcgtctggcg aaggagaaag
     6541 agggcggtgg agagcgtatc gactgacgtg gctatcgtcg gcgcgggcgg cgcggggctg
     6601 cgcgcggcgc tagcgctcgc ggaagcggcg ccgaagctgc gcatcgctct cgtgtcgaag
     6661 gtttatccca tgcgcagcca cacctgcgcc gcagagggcg gcgccgccgg ggtgatcaag
     6721 ccggacgaca gcctccagca gcacttcgac gacactgtcg gcggcggcga ctggctctct
     6781 gaccaggacg cggtcgccta cttcatcgcc gaagccccga aggagctgct gcagctcgag
     6841 cgctggggct gcccctggag tcgcgagtct gacggctcgg tggcggtgcg ccccttcggc
     6901 ggcatgaagc tcatgcgcac ctggttcgcc gccgacaagt cgggcttcca catcctgcac
     6961 acgctcttcc agacctcgct caagtatccc tcgatccagc gtttcgacga gtattacgtc
     7021 accgaacttc tggtggaaga cgggcgttgc cgcggcctca ctgccatcga gatgcggagc
     7081 ggtcagatgc gccgctttga cgccgccgcg gtgatcctcg ccgggggcgg ggcggggcgc
     7141 atgttcccgt tcaccacgaa cggcgcgatc aagaccggcg acgccatggc cctggcctac
     7201 catgtcggcg tgccgctcaa ggacatggag ttcgtgcagt accacccgac cggcctgccc
     7261 agcacgggca cgcttctgac cgaagcctgc cggggcgaag gcggcgtgct gctcaacaag
     7321 catggccgcc gcttcctgca ggattacggc atgggaccgg agacgccgct cggcaaaccg
     7381 gtgctcaaga cgatggagct cgggccgcgc gaccgtgtaa gccaggcgtt ctggcacgag
     7441 cagcgcaaag gcaacgcggt gagcacgcgc tggggcgacg cgatgctcct cgatatgcgc
     7501 cacctcggcg agaagacgat caacgagcgg ctgccgctgg tgcgcgacat gtccatcagc
     7561 tacatggggg tcgatccggt cagcgacccc gtgcctgtgc gcccggtggt gcattacatg
     7621 atgggcggca tccataccga catcaaggcg gcgaccccgg tcgccgggct gttcgcagcg
     7681 ggcgaatgcg catgcgttag catcaacggc gcgaaccgcc tgggctccaa ttcgcttacc
     7741 gagctgctgg tcttcggccg gcgcgcggct ttgagcgcac tcgagttctt gcagaagggc
     7801 ggcgcccgcc ccgcgtccgc atcgctcgcc gacaacgcgc aagggaaaat ccgcgcgttg
     7861 ttccagcgtt cggggggcgg cgagacggtc gccgggctgc gcaaggaaat gctgaacacc
     7921 atggaagagc acgcgggcat ctaccgcagt ggcgagggcc tggccgcggc ctgcgccaag
     7981 ctcggcgagc tgcggcggcg ctaccgcaac ctcgagctgc acgacaagac caacgtctac
     8041 aacaccgatc tcctccaggc gctcgagctt ggctcgatgc tcgactgcgc cgaagccgtg
     8101 gttcagagcg cggctgcgcg caaggagtcg cgcggcgcgc accagcggct ggattttccg
     8161 gagcgggagg accgcaccta cttgcgccat tcgatggcca cctacgctgg cgatggtccg
     8221 ccgcatattg gctaccgcga cgtggtgatc acccgttccc agcccggagt gcgggactac
     8281 tctggagcgc acgcgtgagg aaggtggagc tcgaggtatt gcgctacaac ccggaaacgg
     8341 accgcgagcc gcatttccag cgctattcgg tcgactgcca ggaagaatgg gtggttctag
     8401 acgcgctcaa ccatgtcaag gagacgctcg acccgacgct gagctaccgc tggtcctgcc
     8461 acatggcggt gtgcggaagc tgcgggatga tgatcaacgg ggagccgaag ctcgcctgca
     8521 aggcattcct gcgcaattac gagggcacga tccgcgtgga gccgctgcgg cacttcccgg
     8581 tcgagcggga tctcgtgacc gtgatcgacg acttcgtggc caagctcgct cgcgtcaagc
     8641 cctggctgat gccgaaggag caagcgcccg cgacgggtga ataccgccag acacccgcgc
     8701 aactgaagcg ctacaagcag ttcacgctgt gcatcaactg catgctgtgc tacgcggcct
     8761 gcccggaata cggcctgatc cccaaataca tcggtccggc ggcgatcgcg ctcgcgcacc
     8821 gctggaaccg cgactcgcgc gacggcggcc gcgatgcgcg ccaggaggag atcgcatcca
     8881 aggagggcgc gtgggagtgc tccttcgtcg gcgcctgctc ggaagtctgc cccgagcatg
     8941 tggatccggc gggcgcgatc cagcaggtca agatcgagag cacggtcgat tggtaccagg
     9001 cgcatctgat gccctggttg aacaaatgag tcgcaacccc tacgtccgcc cgatcacctc
     9061 cgagtggatc ttccgccacc cgcgctatgt gcgctatatg gtgcgggagt tcagctgcct
     9121 gttcatcggc ggctggacag cgctaatggt gtggggcctg aagcagctct cggaggggcc
     9181 cgccgcctgg gcg
//
LOCUS       DASTPD010000347         6334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1825335, whole genome shotgun sequence.
ACCESSION   DASTPD010000347 DASTPD010000000
VERSION     DASTPD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825335"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1020)
                     /gene="epsA"
                     /locus_tag="VFR57_11745"
     CDS             complement(55..1020)
                     /gene="epsA"
                     /locus_tag="VFR57_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HEU0260074.1"
                     /translation="MNTVQVKFPGWLGIHSAETTSWPARAKPSAEVRVKGFEFSPEDA
                     ARFIHIVSECGRIRTHYDIYRLLSGELQHFLPHHILLSAWGDFAAFDLKLDLTSSMPG
                     VRTAQLAHCRVDRLVRKAYTRWIEAGRKPVLVKPAEVDLAQPCHCAIHTALRDMRAVL
                     VHGVHDKRSGRDSLFIVFGTEPFTKSRSEPRFLSCLDPLFAQIDAAFRKVPVYSLPAS
                     HDGVNVLELSARELDVLEAITKGKTNLDIATALDISPVTVKNHVQRIFRKIGVTNRTQ
                     AAARYTEAVRKLAQCAAANAAPAAQVGSMSVAFAQPSAAYAPAGA"
     gene            complement(1035..1358)
                     /locus_tag="VFR57_11750"
     CDS             complement(1035..1358)
                     /locus_tag="VFR57_11750"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0260075.1"
                     /translation="METRAFMAARREERFATELPMRFEGGGTGVVRNVSASGVYLATD
                     VALEEGQAVSFTVEFRDLPSGPIAVNCVALIVRREEQGAGRGVGASISSFEFRRLDAP
                     GKSSR"
     gene            1520..2005
                     /locus_tag="VFR57_11755"
     CDS             1520..2005
                     /locus_tag="VFR57_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0260076.1"
                     /translation="MDMPALHCLACEHENPRGENICQKCGSTLNLKLCKQCEAINEIK
                     APACHKCGIQFGSARLTRARTAAIAAGVCTVLAVSGLAYQFVYRQPVAGASNVARQPA
                     VGPIVQPVATTPAEDKAAATTAPEKKKPATPAPAPATSGTTRTAVTHTRPAGSQHPRQ
                     W"
     gene            complement(2191..2886)
                     /locus_tag="VFR57_11760"
     CDS             complement(2191..2886)
                     /locus_tag="VFR57_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0260077.1"
                     /translation="MGIQIADRGIRVMLVDSHRLAVCGLQRVIDDEKPDLEVVATATT
                     CSEAILVAEQAQPDVVVLDADLAGQNEAAVVPKLVNGRSTRVLLLGDAAADLTHELAI
                     LRGACGVVRKDETAATLIKAIRKVHEGELWLDRSTTGRLFVELSKQNNASLSGRSDFG
                     LQSLTSREQDVVRSLVSQPEADNKTLASSLHIGEHTLRNHLSRIYDKLGVPNRVALYV
                     LAERHGLTVRQGR"
     gene            complement(3182..4186)
                     /locus_tag="VFR57_11765"
     CDS             complement(3182..4186)
                     /locus_tag="VFR57_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260078.1"
                     /translation="MRFFGLLLLAAPLGASAQLTVYCSIIDEQCRAGVAAFERSSGVK
                     VTMVRKGTGETYAQIRAEASNPRGDVWWGGPGDPHMQAAEEGLLEEYKSPRLAELHPW
                     AVRLAELSKFRTTGIYLGALGIGYNTQVLKKKNIPEPKCWADLLDAKLRDEVQIADPN
                     SSGTAYVMLATVVQLMGEDKGFAYLKSLHRNVNQYTKSGIAPVKATASGETGVGIAFL
                     HDMVTMRVQGAPIATVAPCEGTGYEIGAVSLIKGRKNSEAAKRFLDWTLTAAAQKIVG
                     VDQNIYSIPSNKAAPVHPQAPNIASIKLINYDSAKYGSSAERNRLLKKWGDEVKALPK
                     "
     gene            complement(4197..5168)
                     /locus_tag="VFR57_11770"
     CDS             complement(4197..5168)
                     /locus_tag="VFR57_11770"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU0260079.1"
                     /translation="MKKIAVLTSGGDAPGMNAAIRAVTRGALAHGWEVFGVRNGYAGL
                     LGDALEPLLARDVGGIVQAGGTMLGSARCPEFAEPAGRSKALENLKTRGIHALVVIGG
                     NGSQSGSASLAREGFAVVGVPSTIDNDLYGNDVSIGCDTAINITLEAIDRLRTTASSH
                     QRAFAVETMGRDCGYIALMAGIAGGAEVIALPESEITPAQLAERLRDAYRRGKTHALA
                     VIAEGAKCGVHELMEYYKANRELISYDLRVTRLGHVVRGGTPGAADRVLATRLGAAAI
                     DALANGQHGVLVGIVKNDTVYTPLAEITGRTRPADASLLELARVMAI"
     gene            5189..5788
                     /locus_tag="VFR57_11775"
     CDS             5189..5788
                     /locus_tag="VFR57_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEU0260080.1"
                     /translation="MAKSPLDGLRQAARRCQDCPLWANATQTVFGAGDPHARVMLVGE
                     QPGDEEDKKGLPFVGPAGRLLDKALEAAGVDRDHLYVTNAVKHFKWQLRGKRRLHKTP
                     AQREIDACHQWIEGEIKAVKPHVIVALGATAAKALIGPQFKVTLQRGQFVESPLAPYV
                     FATFHPSALLRLQEDEDREAAFQQFVKDLKLIKKALSRP"
     gene            complement(5774..>6334)
                     /locus_tag="VFR57_11780"
     CDS             complement(5774..>6334)
                     /locus_tag="VFR57_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0260081.1"
                     /translation="KALGKEVRFANDANCFALSEAADGAARGARVVFGAILGTGAGGG
                     LVVNGQVIEGRHGTAGEWGHTPLPWMREDEYPGPRCNCGRIGCIEQFVSGPAKNRERD
                     AMGEEAAMTRFVDRLARAFAMMINIIDPDVIVVGGGMSKHDEIYTQVPPLLRKYTYHP
                     DPRTPVVRASHGDASGVRGAAMLWPA"
BASE COUNT         1065 a         2085 c         2039 g         1145 t
ORIGIN      
        1 gaagcgagct aactccttag cccgaaagag ccaattcttc gcgcgcagcg aggatcaggc
       61 gcccgcgggc gcgtacgctg ccgacggttg cgcaaaggcg accgacatcg atcccacctg
      121 ggcagccggc gcggcgttgg ccgccgcaca ctgcgccaac ttgcgcacgg cttcggtgta
      181 gcgcgccgcg gcctgggtgc ggttggtgac gccgatcttg cggaagatgc gctgcacgtg
      241 gttcttgacc gtgaccgggc tgatgtcgag cgcggtcgcg atatcgaggt tggtcttgcc
      301 tttggtgatc gcctcgagca cgtcgagctc gcgcgcgctg agctccagca cgttcacgcc
      361 gtcgtgcgag gcgggcagcg agtacacggg aaccttgcgg aaagccgcat cgatctgcgc
      421 aaacagcgga tccaggcacg aaaggaagcg cggctcgctg cggcttttcg tgaacggctc
      481 ggtgccgaag acgatgaaca ggctgtcgcg cccggagcgc ttgtcgtgca cgccgtgcac
      541 gagcaccgcg cgcatgtcgc gcagcgccgt gtgaatggcg caatggcagg gctgcgcgag
      601 gtccacttcg gccggcttga cgagcacggg cttgcggccg gcttcgatcc agcgcgtgta
      661 cgccttgcgc acgagccgat ccacgcggca gtgggcgagt tgcgcagtgc gcacgcccgg
      721 catgctggaa gttaggtcga gcttgagatc gaaggccgca aaatcccccc acgccgaaag
      781 caggatgtgg tgcggcagga agtgttgcag ctcgccgctc agcaagcggt agatgtcgta
      841 atgcgtgcgg atgcggccgc actccgagac gatgtgtatg aatcgcgcag cgtcttcggg
      901 actgaactcg aagcccttga cccggacctc cgccgacggc ttagcccgcg cgggccagct
      961 cgtcgtctct gcgctgtgga tacccagcca acccggaaac ttcacttgga ccgtattcat
     1021 gtcttggacc tttcttagcg ggagcttttg cccggcgcgt cgagacggcg gaattcgaag
     1081 ctgctgatcg aagcccccac gccacgcccg gctccctgct cctcccgtcg cacgatcagt
     1141 gcgacgcagt tcactgcaat ggggccgctc gggaggtcac gaaactccac cgtgaagctc
     1201 accgcctgcc cctcttctag cgccacgtcg gtcgcgaggt acacgccgct cgcgctgacg
     1261 tttcggacca cgccggtccc accaccttcg aaccgcatcg ggagctcggt tgcgaaacgc
     1321 tcctctctac gtgcggccat gaaggcccgg gtttccatgg cgcctagggg cgacatattg
     1381 gatgagacgc gcccgtcgcg agcgagcgac aagtactgtt tacgttggtg cttttgtcat
     1441 gggacagatg tcaccggcac gaaatagcta gtccgcagcc gcgtgtggcg cctttctata
     1501 atcggcgagg aagagatcca tggatatgcc ggccctgcac tgcctcgcgt gcgagcacga
     1561 gaacccgcgg ggcgaaaaca tttgtcagaa gtgcggctcg acgctcaacc tcaagctatg
     1621 caagcagtgc gaggccatca acgagatcaa agcgccggcg tgccacaagt gcggcatcca
     1681 gttcggttca gcgcgtctta cgcgtgcgcg cacggcagcg atcgcggcag gggtgtgcac
     1741 cgtcctcgcc gtgagcggtt tggcctatca gttcgtctat cgccagccgg tcgctggtgc
     1801 gtcgaatgtt gcacgccaac ccgcggtagg gcccatcgtg cagcccgtgg caacaacgcc
     1861 ggcggaagac aaggcggccg cgaccaccgc tcccgagaag aaaaaacctg cgacgcctgc
     1921 cccggcgccg gccacgtcag ggacgacgcg gacggcagtc acgcatacgc gtcctgctgg
     1981 tagccaacac ccgcggcagt ggtgacccta gcttgggagg gatgtcggcg gcgtttacgt
     2041 ttggtgtcgg ccgcgacgca aacccttgaa tcttcgacgt ggtccgatta ttgcggcaga
     2101 cggtttcgag cgattggacc cgctcgacct tggccccgca tgagcccccc tcttcggggc
     2161 ttttcttttt ctaccgcacg cccggcttga tcagcggccc tgccgcaccg tgagaccgtg
     2221 gcgctcggcc aggacgtaaa gggccacgcg attgggtacg ccgagcttgt cgtagatacg
     2281 gctgagatga ttacgcaagg tgtgctcgcc gatatggagg cttgacgcca gagtcttgtt
     2341 gtccgcttcc ggctggctca cgagagagcg caccacatcc tgctcgcgcg aagtaagcga
     2401 ctgcaggccg aagtcagacc gaccagacag cgacgcgtta ttctgcttgg atagctctac
     2461 gaacaacctt cccgtggtcg agcggtcgag ccacagctcg ccttcgtgca ctttcctgat
     2521 cgctttgatg agggtggcgg ccgtctcgtc cttgcgcacc acaccgcacg cgcctctcag
     2581 aatggcgagc tcatgggtaa ggtccgccgc agcgtcgccc aggagcagca ctcgagtgct
     2641 gcgcccgttg acgagtttgg gcaccaccgc cgcctcgttc tggcccgcga ggtctgcgtc
     2701 gagcaccacg acatcaggct gcgcttgctc ggccaccaag atggcctcgc tgcaggtggt
     2761 cgcggtcgcg accacttcga gatccggctt ctcgtcgtcg atgacgcgct gcagcccgca
     2821 aaccgctagc cgatggctgt cgaccagcat gacacggatc ccccgatccg cgatctgtat
     2881 acccatgcgc ctccctagac gccggatgat agcgtaggca tgcaaaggga ctggtacttt
     2941 attaccggta atccgtaagg aagtgtctgc agcgtaagtt cggattgtgt cgtgcggatg
     3001 acgcacggcc ccaaggaagc gccttgcgaa aggcagtttc gctgtgctga acaatcgttt
     3061 tagattgcca aaattcgcgc caattccagc aaggtcgtat ccgccgggcg cgtgcgcccg
     3121 gtgatttggg cgagcggcgt atacacgatg tcgttcctta cgacgccggc cggtcatcgc
     3181 gctacttagg cagcgccttc acctcgtcgc cccatttctt gagcaggcga ttgcgctcgg
     3241 cagaagagcc gtatttcgcc gagtcgtagt tgatgagctt gatcgaggcg atgttcggag
     3301 cctgcggatg cacgggcgcc gccttgttcg acggaatcga atagatgttc tgatcgacgc
     3361 caacgatctt ttgcgccgcc gcggtcagcg tccagtcgag gaatcgtttc gccgcctcgc
     3421 tgttcttccg acctttgatc agcgacaccg cgccgatctc gtagccggtg ccctcgcacg
     3481 gcgcgacggt ggcgatcggc gcgccctgca cgcgcatcgt caccatgtcg tgcaggaagg
     3541 cgatgccgac gccggtttct ccggacgccg tggccttcac cggcgcgatg cccgacttgg
     3601 tgtactggtt gacgttgcga tgcagcgatt tcaggtaggc gaaacctttg tcctcgccca
     3661 tgagctgcac gacggtggcg agcatgacgt aggccgtgcc cgacgaattc gggtcggcga
     3721 tctgtacctc gtcgcgcaat ttcgcatcta gcaggtcggc ccagcacttc ggctccggaa
     3781 tgttcttctt cttgaggacc tgcgtgttgt agccgatgcc gagcgcgccg aggtagatgc
     3841 ccgtcgtcct gaacttcgac agctcggcga ggcgcaccgc ccatggatgc agctccgcga
     3901 gccgcggcga cttgtattcc tcgagcagcc cttcctcggc cgcctgcatg tgcggatctc
     3961 cgggaccgcc ccaccagacg tcgccgcgcg gattcgacgc ctcggcacgg atctgcgcgt
     4021 aggtttcgcc cgtgcctttg cgcaccatgg tgaccttcac accgctcgag cgctcgaatg
     4081 ccgcgacgcc ggcgcggcat tgctcgtcga tgatcgagca gtagaccgtc agctgcgcgg
     4141 acgcgccgag cggcgcggcg agcagcaaca atccgaaaaa gcgcatttcc cctcccctag
     4201 atcgccatca ccctcgcgag ttcgagcagg ctagcatccg ctgggcgcgt gcgcccggtg
     4261 atttcggcga gcggcgtgta cacggtgtcg ttcttcacga tgcccacgag cacgccgtgc
     4321 tggccgttcg ccagcgcgtc gattgccgcg gcgccgaggc gcgtggcaag gacacggtcc
     4381 gctgcgccgg gcgtgccgcc gcgcaccacg tgaccgaggc gcgtcacgcg caggtcataa
     4441 ctgatcagtt cgcggttggc cttgtagtac tccatgagct cgtgcacgcc gcacttcgcg
     4501 ccttcggcga tcacggcgag cgcatgcgtc ttgccgcgcc ggtatgcgtc gcgcagccgc
     4561 tcggcgagct gcgccggggt gatctcgctc tcggggagcg cgatcacttc cgcgccgccc
     4621 gcgatgccgg ccatgagggc gatatagccg caatcgcgac ccatcgtctc caccgcgaag
     4681 gcccgctggt gcgacgacgc ggtggtgcgc aggcggtcga tcgcttcgag cgtaatgttg
     4741 atcgcggtgt cgcagccaat gctcacatcg ttgccgtaga gatcgttgtc gatggtggaa
     4801 ggcacgccca ccaccgcgaa gccttcgcgc gcgaggctgg ccgagcccga ttgcgagccg
     4861 ttgccgccga tcaccaccaa ggcatggatt ccccgggttt tgaggttttc caatgctttg
     4921 gatcgtcccg caggctccgc gaactccgga cagcgcgcgc tgccgagcat ggtgccgccc
     4981 gcctgcacga tgccgccgac gtcgcgagcg aggagcggct cgagcgcgtc gccgagcagc
     5041 cccgcgtagc cgttacgcac gccgaagact tcccagccat gcgcgagggc gccgcgcgtg
     5101 accgcgcgga ttgcggcgtt catcccgggc gcatcgccgc cgcttgtcag tactgcaatc
     5161 tttttcaagc cgggcattct aagaccctat ggccaagtcg cccctcgacg ggcttcggca
     5221 ggccgcgcgg cgctgccagg attgcccgct ctgggcgaac gccacgcaga cggtgttcgg
     5281 cgccggcgac cctcatgcgc gcgtgatgct cgtcggcgag cagcccggcg acgaggaaga
     5341 caagaaggga ctgccgttcg tgggtccggc ggggcgtctg ctcgacaaag cgctcgaggc
     5401 ggccggggtc gaccgcgatc atctctacgt caccaatgcc gtcaagcatt tcaaatggca
     5461 gctgcgcggc aagcgccgcc tgcacaagac cccggcgcag cgcgagatcg atgcctgcca
     5521 tcagtggatc gaaggcgaga tcaaggcggt gaagccgcac gtgatcgtcg cgctcggcgc
     5581 caccgcggcg aaagcgctta tcggtcccca gtttaaggtg acactgcagc gcggacagtt
     5641 cgtcgaaagc ccgctcgcgc cttacgtgtt cgcaactttt catccctcgg ccctgctccg
     5701 attgcaggaa gacgaagatc gcgaagccgc cttccagcag ttcgtgaagg acctcaagct
     5761 gatcaaaaaa gcgctaagcc ggccatagca tcgcggcacc gcgcacgccg ctcgcgtcgc
     5821 cgtgcgaggc gcgcacgacg ggcgtgcgcg gatcggggtg gtacgtgtac ttgcgaagga
     5881 gcggcgggac ctgggtgtaa atctcgtcat gctttgacat gccgccgccg acgacgatca
     5941 cgtccgggtc gatgatgttg atcatcatgg cgaacgcgcg tgcgaggcga tcgacgaagc
     6001 gcgtcatcgc cgcttcctcg cccatcgcgt cgcgctcgcg gttctttgcg gggccggaaa
     6061 cgaactgctc gatgcagccg atgcgtccgc agttgcagcg cggacccggg tactcgtctt
     6121 cgcgcatcca aggtagcggg gtatggcccc attcaccggc ggtaccgtgg cggccctcga
     6181 tcacctgacc gttcaccacc aggccgccgc cggccccggt gccgaggatc gcgccaaaca
     6241 ctacgcgcgc accgcgcgcc gcgccgtcgg ccgcctccga tagcgcgaag cagttcgcgt
     6301 cgttcgcgaa gcgtacttcc ttgccgagcg cttt
//
LOCUS       DASTPD010000348         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1827510, whole genome shotgun sequence.
ACCESSION   DASTPD010000348 DASTPD010000000
VERSION     DASTPD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827510"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFR57_11785"
     CDS             <1..677
                     /locus_tag="VFR57_11785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0260082.1"
                     /translation="IGIALLTMFLLRFIRIPTPSAAERSSAGRPLRQIAAQPKYIVAV
                     LSAAIGYGVMNFVMTSTPIAMQACGHAYGDTTFVISSHIIGMFAPSFLSGPLIRRVGA
                     LPVMFTGALLNFAAIGVALSGIAVTQFWWALVLIGVGWNFLYIGATTLLTETYRPEER
                     ARAQGGNELAIFTVMAISSLSSGFTITNAGWERLNLFALPLIAVVAIAIAWYALGERR
                     RRMAAG"
     gene            674..1765
                     /locus_tag="VFR57_11790"
     CDS             674..1765
                     /locus_tag="VFR57_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU0260083.1"
                     /translation="MIQPAPAAIGQRLDQIDTPALVLDLDVFEKNQTTLFGLVRNRAR
                     VRPHAKTHKCPDVGRRQVALGAVGLCCQKVSEAEAMVDGGIADVLVTNEIVGARKLER
                     LAALSKRAKVGVCVDHAENLKSIAATGAKLDVYIEVDVGMRRCGVPPGEPVVQLARQI
                     QGLKNLRFAGLQAYQGRAQHFRSMEERHAAIEKAAQHVQHTKRLLKEANIACPIVTGA
                     GSGTFMLEVEAGAWDEIQPGSYAFMDADYAKNEWAAPLPRFGHALFVLTTVMSRPSER
                     VAIVDAGLKASSVDSGMPAVWKKDLQYTKASDEHGWVEGRDVPPLGDKLLLVPGHCDP
                     TINLYDWYVCIRKGFVEDLWPITARGAIY"
BASE COUNT          378 a          695 c          680 g          375 t
ORIGIN      
        1 tgatcggcat cgcgctcctc accatgttcc tgctgcgctt catccgcatc ccgacgccga
       61 gcgcggcgga gcgctcctcc gccggccggc cgctgcgcca gatcgcggcg caacccaagt
      121 acatcgtggc ggtgctgagc gcggcgatcg gctacggcgt gatgaacttc gtcatgactt
      181 ccacgccgat cgccatgcag gcctgcggcc atgcctacgg cgacaccacg ttcgtcatct
      241 cgtcgcacat catcggcatg ttcgcgccgt cattcctcag cggcccgctg atccggcgcg
      301 tgggcgcgct gccggtgatg tttaccggtg cgctgctcaa cttcgccgcc atcggcgtcg
      361 cgctgtccgg catcgcggtc acgcagttct ggtgggcgct ggtcctgatc ggcgtgggct
      421 ggaacttcct ctacatcggc gccaccaccc tccttaccga gacctaccgt cccgaggagc
      481 gcgcgcgcgc gcagggcggc aatgagctcg ccatcttcac cgtgatggcg atctcttcgc
      541 tgagctccgg cttcaccatc accaatgccg gctgggaacg gctcaacctc ttcgcgctgc
      601 cgctcatcgc cgtggtcgcg atcgccatcg cctggtatgc gctgggcgag cgcaggcgca
      661 gaatggcggc cggatgatcc agcccgcgcc cgccgcgatc ggccagcgcc tcgaccaaat
      721 cgacacgccg gccctcgtcc tcgacctgga cgtgttcgag aaaaaccaga ccacgctttt
      781 cggcctggtg cgcaaccgcg cgcgcgtgcg gccgcacgcg aagacgcaca aatgcccgga
      841 cgtgggcagg cgccaggtcg cgctcggcgc ggtcggcctg tgctgccaga aagtttccga
      901 agccgaggcc atggtcgacg gcggcatcgc cgacgtgctg gtcacgaacg agatcgtcgg
      961 cgcgcgcaag ctggagcgcc tcgccgcgct gtcgaaacgc gcgaaggtgg gcgtctgcgt
     1021 ggaccacgcc gagaatctga agagtattgc cgccaccggc gccaagctcg acgtctatat
     1081 cgaagtcgac gtcggaatgc gacgctgcgg cgtcccgccc ggcgaacccg tcgtgcaact
     1141 cgctcggcag atccaaggct tgaaaaatct gcgctttgcc ggcctgcagg cctaccaggg
     1201 ccgcgcgcag catttccgca gcatggaaga gcgccacgcc gcgatcgaga aggctgccca
     1261 gcacgtgcag cacacgaagc gattgcttaa agaagccaac atcgcatgcc cgatcgtcac
     1321 cggtgcggga agcggcacgt tcatgctcga ggtggaggcg ggcgcgtggg acgagatcca
     1381 gccgggctcc tacgcgttca tggacgcgga ctacgcgaag aacgagtggg ccgcgccgct
     1441 gccgcgcttc ggccacgcgc ttttcgtgct caccaccgtg atgagccggc cctccgagcg
     1501 cgtggcgatc gtcgacgccg gtctcaaggc ctcgagcgtc gactcgggaa tgccggcggt
     1561 ctggaagaag gacctgcaat acaccaaggc gtccgacgag cacggctggg tggagggcag
     1621 ggacgtcccg ccgttgggcg acaagctcct tctcgtgccg ggtcattgcg acccgaccat
     1681 caacctctac gactggtacg tatgtatcag aaaaggtttt gtagaggact tgtggccgat
     1741 cactgctaga ggcgctatct actagctatg cgggtttgtc cgcgtcagtt cgcaaatgaa
     1801 gaagaaccac agggaatgct gcgatcggcc aaagaatcgt tgggcatcgc ccatggggga
     1861 gttggcgctt agtagcaccc ccacgactag aagatggctg ctaccgagat cgtccaaggc
     1921 gagccgcgag atgccgatga gggcgaagct cctaccgagc gtatattggc gcataagtaa
     1981 acgcgcgaag tagagggagc aatgcggttt tttgcatttg ccctgtcgct tgcggcggga
     2041 tgtgcgtcga atccgctttc taacgcgagg tattggccaa ttgacggctg tacttcgttt
     2101 aacactactc atgtccgctt cacgcgag
//
LOCUS       DASTPD010000349         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1828282, whole genome shotgun sequence.
ACCESSION   DASTPD010000349 DASTPD010000000
VERSION     DASTPD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828282"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFR57_11795"
     CDS             complement(<1..1187)
                     /locus_tag="VFR57_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0260084.1"
                     /translation="MNPGLVLLCGCLVLFLGFGARAGFGLYLQPISLEFGWGREVLSF
                     SLALQQIVWGALGPIAGAIADRYGTGRVIAACGALYVLGLAGMSFAQTPLAMHFAAGL
                     LVGGALAGATFATVLAVIGRSVAPEKRSTALGVATAAGSFGQFALLPVTQLLIGRFDW
                     HVALLVMAGLTALIMPLAAALSGRPVAVAAGQSLGAALREAMRERGFHLLFWGYFVCG
                     FHIAMLTVHLPAFVTDAGLSATHGMTALALIGLFNVVGTLGAGWAGGRFSKKYLLSTI
                     YAVRAVLIAMLVFLPLSPATLYVFACGIGLLWLGTVPLTNGLVGQIFGLRYMATLTAI
                     VFFGHQIGSFIGVWLAGYLYDVTGSYNGAFMTSIALGVFAALVNLPVNEKPLVERRHA
                     QAVA"
     gene            complement(1241..2164)
                     /locus_tag="VFR57_11800"
     CDS             complement(1241..2164)
                     /locus_tag="VFR57_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEU0260085.1"
                     /translation="MTLTELRYIVAVARERHFGRAAETCFVSQPTLSVAVKKLEEELG
                     VTLFERGPGEVSVTPAGEKIVEQAQRVLEEASRIREIAAAGKDPLAGPLRLGAIYTIG
                     PYLLPKLIPILRRTAPAMQLLIQENFTHRLAEELKSGEVDVILVALPFEAPGVVTRAV
                     YDEPFLVAVPKGHPWENRKRVTSDELASESLLLLGEGHCFRDQVLEICHTVRSRERSA
                     LAKTVEGGSLETIRQMVAGGVGVTVLPATSTNASGGASDLIRILPFARPAPTRRVGLA
                     WRRSFPRPEAIEALRKAILACNLPQVEKLPS"
     gene            complement(2161..>2618)
                     /locus_tag="VFR57_11805"
     CDS             complement(2161..>2618)
                     /locus_tag="VFR57_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0260086.1"
                     /translation="IFTGARTNYALGRDFRLFARLGTWRERGSTPANALMLQGVITLV
                     LVAASAATPDGFSAMVAYTSPVFWTFFLLTGLTLFLFRRKGGQHPVFRVPLHPVVPAL
                     FVLMCAYMLYSSIDYVRNPDYGPKFGFAVFAGLVVMAAGIPLYFLSKKK"
BASE COUNT          512 a          884 c          868 g          354 t
ORIGIN      
        1 gccacagctt gtgcatgcct tcgctccacc aacggcttct cattgaccgg caggttgacc
       61 agcgccgcga acactccaag cgcgatcgac gtcatgaagg ctccattgta cgacccggtg
      121 acgtcgtaca ggtaccccgc aagccatact ccgatgaagc tgccgatctg gtggccgaag
      181 aacacgatcg ccgtaagcgt cgccatgtag cgcaggccga aaatctggcc cacgaggccg
      241 ttggtgagcg gcacggtccc cagccagagc aggccgatgc cgcaggcgaa gacatagagc
      301 gttgccggcg aaagcggcag gaacaccagc atggcgatga ggacggcgcg aaccgcgtaa
      361 atggtcgaga gcaaatactt cttgctgaag cggccaccgg cccagccagc cccgagggtt
      421 cccacaacgt taaacagtcc gatcagcgcg agcgcggtca tgccgtgcgt ggcgctcaat
      481 ccggcgtcgg tgacgaacgc cggcaggtgc acggtaagca tggcgatgtg gaagccgcac
      541 acgaagtaac cccaaaacag cagatggaac ccccgctcac gcatcgcctc gcgcagcgca
      601 gcccccagcg actgcccggc cgccacggcg accgggcggc cggaaagcgc cgcggcgagc
      661 ggcatgatga gcgccgtcag cccggccatc accagaagcg cgacgtgcca gtcgaagcgg
      721 ccgatcagga gctgcgtcac cggaagcagc gcgaattgcc cgaaggagcc cgcagccgtg
      781 gccacgccga gcgccgtgct gcgcttctcc ggcgcgacgc tgcgcccgat gacggccagc
      841 accgtcgcga aggtcgctcc ggcgagcgcc ccgccgacga gcaaacccgc ggcgaaatgc
      901 atcgcgagcg gcgtttgcgc gaacgacatg ccggcaaggc cgagcacata aagcgccccg
      961 cacgcggcga tgaccctgcc ggtgccgtag cggtcggcga ttgcacctgc tatcggaccg
     1021 agcgcgcccc acactatctg ctgcagggcg agcgagaagg acagcacctc cctgccccag
     1081 ccgaactcca ggctgatcgg ctgcaggtac aggccgaagc cagcgcgcgc accaaagccc
     1141 aggaaaagga cgagacagcc gcagagaagc acgaggccag gattcacggc ggcagtctag
     1201 cggctggccc gcggaggaac aggcacagtt acaggtgtaa tcaggatggc agcttctcca
     1261 cctggggcag gttgcaggcg aggatcgctt ttcggagtgc ctcgatcgcc tcgggacggg
     1321 ggaagctgcg ccgccaggcg aggccgacac gtcgcgtcgg cgccggccgc gcaaacggca
     1381 ggatgcggat caagtccgag gcgccgccgc ttgcgttggt cgaagtcgcg ggcagcacgg
     1441 tgacgccgac gccgcccgcg accatctgac ggatggtctc gagcgagccg ccctccaccg
     1501 tcttcgcgag cgcgctgcgc tcgcgcgagc gcacggtatg gcagatctcg agcacctgat
     1561 cgcggaagca gtggccctcg cccagtagca gcaggctctc gctcgcgagt tcgtcggacg
     1621 tcacgcgctt gcggttctcc cacgggtgcc ccttgggaac ggcgaccagg aacggctcgt
     1681 cgtacaccgc tcgagtgacc acccccggcg cctcgaaggg tagcgcgacc aggatcacgt
     1741 cgacctcgcc actcttcaac tcttcggcaa ggcgatgggt gaaattctcc tggatgagga
     1801 gctgcatcgc gggcgcggtg cgccgcagaa tcgggattag cttgggcagc aggtaagggc
     1861 caatggtgta gatggcgccc aggcgcagcg gccccgcgag cgggtccttg cccgccgccg
     1921 cgatttcgcg gatgcgcgat gcctcttcca gcacgcgctg cgcctgctcg acgatcttct
     1981 cgcccgccgg ggtcacgctc acctcgcccg gcccgcgctc gaacagcgtc acccccagct
     2041 cctcctcgag cttctttacc gccaccgaca gcgtcggctg cgacacgaag catgtttccg
     2101 ctgcccggcc gaagtggcgc tcgcgcgcca ccgcgacgat gtagcggagc tcggtcaggg
     2161 tcatttcttc ttcgaaagga agtacagcgg tattccggcc gccatcacca cgaggcccgc
     2221 gaaaactgcg aagccaaact tcggcccgta gtccgggttg cgcacgtagt cgatgctcga
     2281 atagagcatg taggcgcaca tcagcacgaa aagcgccggc acgacggggt ggagcggcac
     2341 ccggaacacg ggatgctggc cgcctttgcg gcggaacaga aacaaagtga gccccgtcaa
     2401 gaggaagaac gtccagaaca cgggcgaggt ataggccacc attgcgctga agccgtctgg
     2461 cgtggcagcc gaagcggcga cgagcacgag cgtgattact ccctgcagca taagcgcatt
     2521 ggcgggggtg ctgccgcgct cgcgccaggt tcccagcctg gcgaacaggc ggaagtcccg
     2581 gccgagcgcg taattggtgc gcgcgccggt gaagatcg
//
LOCUS       DASTPD010000350        13652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_191213, whole genome shotgun sequence.
ACCESSION   DASTPD010000350 DASTPD010000000
VERSION     DASTPD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191213"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..540)
                     /locus_tag="VFR57_11810"
     CDS             complement(1..540)
                     /locus_tag="VFR57_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEU0260087.1"
                     /translation="MSARVGVVTLAFAAALGGCAGEEHSDLKQELAQLTKDLRGRVDP
                     LPQVKPYEPVPYTAEGQIDPFRPERIEVAQAGRGAPSASAALLDEQRKRPPEALEAFP
                     LESIQMLGTITQHKETFALVKAGPNLYRVRKGNYMGQNFGVITAIDEAQISLKELVQD
                     STGDWVERVSSLQLVEAKR"
     gene            complement(537..1181)
                     /locus_tag="VFR57_11815"
     CDS             complement(537..1181)
                     /locus_tag="VFR57_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU0260088.1"
                     /translation="MAFNFNSIADEFRRTNWRDAGTWGAAPKAVVLLAILVGVPVAGF
                     FAFNQGQIEELEAGQHKEVKLKEEYVNKKRQAVNLDLHRQQLRDIDAQFGALLKQLPN
                     KSQMDALLVDINQAGLGRGLQFELFKPAAAELRRDFYAELPIDVKVTGNYHDMGAFAS
                     DVGQLSRIVTLKDVSIATAKDGNLTMDAIARTFRYLDDDEIAAQRKAAHKGAKK"
     gene            complement(1184..1786)
                     /locus_tag="VFR57_11820"
     CDS             complement(1184..1786)
                     /locus_tag="VFR57_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU0260089.1"
                     /translation="MTNARVNLLPHRAERRQRARKHFLTLSGMTVGLGVLIAFLIYQF
                     YASQIEVQNDRNTFLANEIKKLDKDIADINELKNQIQALLARKQIIETLQAERAQTVH
                     LLEQMVRQMPEGVYLRSMRQSGPRVHIVGYAQSNARVSTLMRNIEASPWLEQPVLVEV
                     KASNVDKKRVSEFNMYLNLKQRAASPDGEKDAAKSAAKKG"
     gene            complement(1799..2875)
                     /locus_tag="VFR57_11825"
     CDS             complement(1799..2875)
                     /locus_tag="VFR57_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEU0260090.1"
                     /translation="MKFDLFTAKAPPLFGLDISSSSVKMLELADNGKGSYRVERYAIE
                     PLPRDCVVDGNINNLEAVTDAVKRAHARMGTRTRHVAMAVPSGAVISKKIIVPAALRE
                     EELEVQVESEANQYIPFALEEVNLDFQILGPSPDNPEEQEVLIAATRKEKVEDRVAVA
                     DSAGLKPLVMDVESFAQQSALSFVVQGLPGGGKDQNVAVVDVGANVMNVSVLRNEQSV
                     YTREQAFGGNQLTQDIVNRYGMTPDEAENAKRTGALPDDFDTEVMKPFMENLSMEVQR
                     ALQFFFTSTQYHAVDHILLAGGSAVIPGLDEVVHTRTQVPTSVANPFALMQTSPKVQL
                     KRLMADAPSLIVACGLAMRRFDPQ"
     gene            3064..5370
                     /locus_tag="VFR57_11830"
     CDS             3064..5370
                     /locus_tag="VFR57_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU0260091.1"
                     /translation="MWIRFVAFPLAVLAGLVVAALLAAGLVVVLAYPQLPSLEALTAY
                     QPKIPLRIYTAEGIMIGEFGEERRAVISIADAPQQLKNAVIAAEDERFYEHPGIDYVG
                     VMRAAWANLVAGGRRQGASTITMQVARNFFLSSEKTLTRKLYEALLAFKIEHSLTKEQ
                     ILELYVNQIYLGHRAYGFAAASQIYYGKALGQLSLAETAMLAGLPKAPSLYNPISNAQ
                     RAKVRQQYVLRRMTELGYIDATQYEDALKAPVRTRREVTEYSVHAEYVAEMVRQALAE
                     HYPEDVYTRGFRVYTTIRKADQEAAYEALRRGVLEYDRRAGYRGPEGYVELPANPNDD
                     DIEDALADRPDNDDLIAAVVLAHDGKQVQAGLRTGERISIQGAGLQFAARALDAKAPP
                     QRRIRKGAIVRVQRDGKNWQIAQLPEVEAAFIGIDPQDGAVRALVGGFDFSRNKFNHV
                     TQAWRQPGSSFKPFIYSAALEKGFTAATVVADEPVVLEAEQTGSQRWEPKNYDGKFEG
                     PMRLRTALAKSKNMVSIRVLDAIGPKYAQDYVTRFGFEAERHPAYLTMALGAGSVTAW
                     QMARAYAVFANGGFLVQPYFIHKIVDDRGNALAVAEPRRAGDEVLRVLDPRNAFIMDH
                     MMQDVTRAGTAARAARLGRSDLAGKTGTTNEFVDAWFAGYQPALVGISWVGYDQPRTL
                     GKNQTGGVVALPIWLGYMERVLSDYPEMARTMPPGVVAVPTMALSGEPKMIPEFFYRE
                     AIPPPDVLTPPPPPTAPTPLPEQSNPPA"
     gene            complement(5341..5655)
                     /gene="cyaY"
                     /locus_tag="VFR57_11835"
     CDS             complement(5341..5655)
                     /gene="cyaY"
                     /locus_tag="VFR57_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEU0260092.1"
                     /translation="MDDHRFEVAAEEALERLEHALEGLELDFERKEGGVLEIEFDDGA
                     KMIVNRHSAARELWVAARSGGFHFRWDGSAWRDTRSGEEFFAALSALLSRELGRGVRL
                     LG"
     gene            5675..5842
                     /locus_tag="VFR57_11840"
     CDS             5675..5842
                     /locus_tag="VFR57_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEU0260093.1"
                     /translation="MRFVVLLAACALAAGCGQKGPLFLRESPPPGVKPPKPKPYEPVP
                     YPPELTEEEKK"
     gene            5850..7079
                     /gene="lysA"
                     /locus_tag="VFR57_11845"
     CDS             5850..7079
                     /gene="lysA"
                     /locus_tag="VFR57_11845"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU0260094.1"
                     /translation="MPFNYRHSVLCADEVALDEIAERFGTPCFVYSAASIAASYAEFE
                     DALKGRAARICYSVKANSNLAVLALLAKLGAGFDIVSGGELARVLAAGGDARSTLFSG
                     VGKSEAEIFFALKKGIGCINIESEAELDRVQAVAASLRLRAPVALRVNPDIDANTHPY
                     ISTGLSHTKFGVPHAEAESLYRKAADMPNIDVVGIGCHIGSLLLEAAPFIAATERLLL
                     LVDRLDSAGIRLRHVDVGGGIGIRYKDESPQPPEAFVRGVLAALGRRPHTVIFDPGRS
                     IVGNAGLLLTRVEYLKPGFLVVDAGMNDLIRPALYGAWHEVRAVRESASTTGVYDIVG
                     PVCESADFLAKGRPLAARAGDLLAVMSCGAYAMAMSSNYNTRPRAPEVLVRDGRALLV
                     RRRESVEELFALERRPD"
     gene            7094..8260
                     /locus_tag="VFR57_11850"
     CDS             7094..8260
                     /locus_tag="VFR57_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0260095.1"
                     /translation="MTTATRRLTLLGICVALIAGAVAAYFYAADGRAARKEIAKTAAK
                     GAGQAVLITTAPVTPRTLEIYEEVLGTIENVIDPTIGAEVAGRVTRVTGFTGRKVKKG
                     ELIAEIDAADFQIQQRADQAEIGRLTSLLEQQDRVVERQQKLVAQGFISKNAIDDAVA
                     QRNALRQSLAAARARSDATGRSVTKARVVAPVDGEIETQIVAIGDYVKLGDPLFRMVG
                     VQELRVHLLLPEAAATRIKPGLKVVLSPPAAPERVLEARIDEIKPTVGTNNRALDAIV
                     KIRGEGTLLRGGGTVNARIVTSSLDGALMVPEQSVVLRPAGKVVYAIEGSVARQRVVE
                     TGLRQDGWQQVVKGLSAGETVAADGAGFLTDGAAVTVRAKPGAVAEEGTKKGGS"
     gene            8257..11331
                     /locus_tag="VFR57_11855"
     CDS             8257..11331
                     /locus_tag="VFR57_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0260096.1"
                     /translation="MTLSELSIRRHVFAFMLSAVLVLFGAIAYQRMGIDKLPYIEFPV
                     ISITTAQRGANPDIVDASITNLIETAVNSVPGIEHIQSTSSPGTSVVVITFGLEKRID
                     VAFQEVQAKVNQVVRRLPKDADPPVVAKVETNTQPILWTALQGDRTQQQLNQYAINVI
                     KKQLETIDGVGEIRIGGRRDRVIRVSLLPAKLAAYGIAAQDISDAFGKEHLQLAGGFL
                     VGRSTEHLLKLDLEFHKLDDLAAMIVGYKDGAPVRLRDIAEVEDGLADFRQLARFNGK
                     PAVGIGVVKVPNTNTVAIIDKVLERIEKDVRPQLPPGLELHYVQNDKVFIGAMVESLK
                     QHLVEGTILAALIVWLFLKSFRSTVIIACSIPVSLMGAIAVMYFFGYTFNSVTLLALL
                     LLVGVVVDDSIVVLENIYRHREEDRETSALAAAVTGSREVTFAVIAASFSLVAIFAPV
                     VFVSGILGQFLRSFAVVVTFGVLVSLFVSLTLTPMLCSRYLDVKDKHGRVYWVLERFF
                     HGLDGLYRRLLAWSLRNRGLVLGGAMLCFVASLPVFMQLPRELAPQADEGRFLVGIRT
                     PLGSSIEYTESKLREVEQIAFRYPEIVTEFGVIGLGSAQQVNQATLVARMKPRAERQR
                     SQAEVINAIRRDLQQVAGARAFPRAFGLVQGQRSEPLQFVVKGPNLQEMGRLAAELQR
                     TLQADPQIGRMDTDLQLDLPQLVLEPDRLRAAGLNLTSGDVALAVSMLTGGIDIAKYN
                     DYPGDGERYDIRVKAKDGEFQQQADLAKIYLRSRQGQLVRLDQVARFREALGPAVIAR
                     FDLQYSASFYASPTIALGAAMEKVRAAAQNLPIGYRVVFVGEAEQLEKTANATQFTFA
                     LALALLFMVLASQFNSFLQPFYIMLAVPLGIVGGIAALWLSGSTLNIYSGIGLVLLIG
                     LVAKNSILLVDLTNQRREQGLAVDEALRDACPTRMRPVVMTSLTIILALAPAAIGLGA
                     GAETNAPLSIAVIGGMITSTLLTLIVVPAGYSLVENWRQRRSAARFASGTAD"
     gene            11333..12133
                     /locus_tag="VFR57_11860"
                     /pseudo
     CDS             11333..12133
                     /locus_tag="VFR57_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            12027..12659
                     /gene="mug"
                     /locus_tag="VFR57_11865"
     CDS             12027..12659
                     /gene="mug"
                     /locus_tag="VFR57_11865"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEU0260097.1"
                     /translation="MASVPSPTSSRKPKRKASSSSKPTRSPAATWSGPRSKAELQAAA
                     GRGLRDVIGPRLKVLFCGINPGLYSAATGFHFARPGNRFWPALHAAGFTPRLLHPSEN
                     ERLLELGYGLTNLVNRGTAAADELSTGELIRGGRGLAAKVRRFRPRAVAFVGLGAYRV
                     AFGRPRATLGLQPEPLEGADVWVLPSPSGLNANYQLPALVKLFRALRMRS"
     gene            12662..12856
                     /locus_tag="VFR57_11870"
     CDS             12662..12856
                     /locus_tag="VFR57_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260098.1"
                     /translation="MATKLDKSLKREIEIDGQAYMLTIDPAGMKLVPKGKRNGLELAW
                     KALISGDAALASALNASLKV"
     gene            12837..13445
                     /locus_tag="VFR57_11875"
     CDS             12837..13445
                     /locus_tag="VFR57_11875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0260099.1"
                     /translation="MPLSKSDDLHGAAPDKCAVALVLIDWINDLEFDSGAKLLPEALK
                     AAKATAALRKRAKQAGVPVIYCNDNFGKWRSDFRSLLEHCLKGEVRGRAIAELLAPDE
                     EDYFVLKPKHSGFHSTTLDVLLAHLGSQTLVLTGIAGNFCVLFTAQDAYMRDFKLVVP
                     RDCCASEEESDNRHALEHMAKVCKADTRRSTDIDFNSLRKAA"
     gene            complement(13442..>13652)
                     /locus_tag="VFR57_11880"
     CDS             complement(13442..>13652)
                     /locus_tag="VFR57_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU0260100.1"
                     /translation="CNRGTRVTLYVRSDMGSNRSTAFRYAREANVGVFYWVDTKIGYA
                     VSSADISKEDLLNVANAAYQQLNPP"
BASE COUNT         2283 a         4493 c         4422 g         2454 t
ORIGIN      
        1 tcatcgtttt gcctccacca gttgcagcga gctcacgcgc tccacccaat cgccggtcga
       61 gtcctggacc agctccttca ggctgatctg ggcctcgtcg atcgcggtga tgacgccgaa
      121 gttctgcccc atgtagttgc cttttctcac ccggtacagg ttcggaccgg ccttgacgag
      181 cgcgaaggtc tccttgtgct gggtgatggt gccgagcatc tggatcgact cgagcgggaa
      241 cgcctcgagc gcctccggcg ggcgttttct ctgctcgtcg agcagcgctg ccgaggcgct
      301 cggcgcgccg cgtccggcct gcgccacttc gatgcgctcg ggcctgaacg ggtcgatctg
      361 gccttctgcg gtatagggca ccggctcgta gggcttcacc tgcggcagcg gatcgacgcg
      421 cccgcgcagg tctttggtga gctgggccag ctcctgcttg aggtcgctgt gctcctcacc
      481 ggcgcagccg cccagcgcgg cggcgaaggc aagcgtcacg accccgactc gcgcgctcat
      541 ttcttcgcgc ctttatgggc cgccttgcgc tgcgcggcga tttcgtcgtc gtcaagatag
      601 cgaaacgtgc gcgcgatcgc gtccatcgtc aggttgccgt ctttggccgt ggcaatgctc
      661 acgtccttca gcgtgacgat gcgcgaaagc tggcccacgt cactcgcaaa ggcgcccatg
      721 tcgtgatagt tgccggtgac tttcacgtcg atcggcagct cggcgtaaaa atctcgtcgc
      781 agctccgcgg cggccggctt gaagagctcg aactgcaacc cccggcccaa ccccgcttgg
      841 ttgatgtcga cgagaagcgc atccatctgc gacttgttcg gcagctgctt cagcagcgcg
      901 ccgaactgcg cgtcgatgtc gcgcagctgc tggcggtgca ggtcgaggtt caccgcctgc
      961 cgcttcttgt tgacgtactc ctccttcagc ttcacttcct tatgctggcc ggcttccagc
     1021 tcttcgatct gtccctggtt gaaggcgaag aagccggcca ccggcacgcc gaccaggatg
     1081 gcgagcaaca cgacggcctt gggcgccgcg ccccaggtgc cggcgtccct ccagttggtg
     1141 cgcctgaatt cgtcggcgat ggagttgaag ttgaacgcca tggctatccc ttcttcgccg
     1201 cactcttcgc ggcgtccttc tcgccgtcgg gcgacgctgc gcgctgcttt aggttgagat
     1261 acatgttgaa ttccgagacg cgcttcttgt cgacgttgct cgccttgacc tccacgagca
     1321 ccggctgctc gagccagggc gaggcttcga tgttgcgcat gagcgtcgaa acgcgcgcgt
     1381 tcgactgcgc atagccgacg atatgaaccc tgggcccgct ctggcgcatc gaccgcaggt
     1441 aaacgccctc gggcatctgg cgcaccatct gctcgaggag atgcacggtc tgcgcccgct
     1501 ccgcctgcag ggtctcgatg atctgcttgc gcgcgagcag cgcctggatc tggttcttga
     1561 gctcgttgat gtcggcgatg tccttgtcaa gcttcttgat ctcgttcgcg aggaaggtgt
     1621 tgcggtcgtt ctgcacctcg atctggctcg cgtagaactg gtagatgagg aaagcgatga
     1681 gcacgcccag cccgaccgtc atccctgaaa gcgtgaggaa gtgcttgcgc gcgcgctggc
     1741 ggcgttccgc ccggtgcggc agtaggttga cgcgtgcgtt agtcatgtgg atcgatgttc
     1801 attgcggatc gaacctcctc atggcaagcc cgcaggcgac aatgagcgag ggggcgtcgg
     1861 ccatcaggcg cttgagctgg accttgggcg aggtctgcat cagcgcgaac ggattcgcga
     1921 cgctcgtcgg cacctgggtg cgcgtatgca ccacctcgtc gagccccgga atcaccgccg
     1981 agccgccggc gagcaggatg tggtcgacgg cgtggtattg cgtggacgta aagaagaact
     2041 gcagcgcgcg ctgcacctcc atggaaaggt tctccatgaa tggcttcatc acttcggtgt
     2101 cgaagtcgtc gggcagcgcg ccggtacgct tggcgttctc cgcctcgtcg ggcgtcatgc
     2161 catagcggtt gacgatatcc tgcgtgagct ggttgccgcc gaacgcctgt tcgcgcgtgt
     2221 acaccgattg ctcgttacgc agcaccgata cgttcattac gttggcgccg acgtcgacga
     2281 cggcgacgtt ctggtccttg cccccgcccg gcagcccctg cacgacgaaa gaaagcgcgc
     2341 tttgctgcgc gaacgactct acgtccatga cgagtggctt aaggcccgcc gagtcggcga
     2401 cggcgacgcg gtcctcgacc ttttccttgc gcgtcgcggc gatgagcacc tcctgctcct
     2461 ccgggttgtc cggcgaaggg ccgaggatct ggaaatcgag attgacttcc tcaagcgcga
     2521 acgggatgta ctgattcgcc tcgctctcga cctgcacttc gagctcttct tcgcgcagcg
     2581 cggccgggac aatgattttc ttgctgatta ccgcgccact cggcaccgcc atggcgacat
     2641 gccgagtgcg cgtccccatg cgggcgtggg cgcgcttgac cgcgtcggtg accgcttcga
     2701 ggttattgat gttgccgtcg accacgcagt cgcgcggcag cggttcgatc gcgtaacgtt
     2761 cgacccggta gcttcccttg ccgttatcgg cgagctcgag catcttcacc gacgaggagc
     2821 tgatgtccag gccaaacagc ggtggcgctt tcgcggtgaa caggtcaaat ttcactttgt
     2881 gtctccccgt atttcccttt gtgcgtcggc tcttaggcgc gattcgcaga aattgcgtta
     2941 aatgctagca acaagccccc cgactgtcta gttaattgtc ctccggggtt cgggttatga
     3001 tgccggcgtt ccggcaaccc catgcaacaa cggccgtccg cgcggctcaa agcacctaag
     3061 aagatgtgga tccggtttgt cgccttcccg ctggcggtgc tggcgggcct cgtcgtcgct
     3121 gcgctgcttg cggccgggct cgtcgtggtg ctcgcgtacc cgcagctgcc ctcgctggaa
     3181 gccttaaccg cctaccagcc gaagatcccg cttcggatct ataccgcgga gggcatcatg
     3241 attggcgagt tcggcgagga gcgccgggcc gtgatctcga tcgccgacgc tccgcagcag
     3301 ctcaagaacg cggtgatcgc cgccgaggac gagcgcttct acgagcaccc cggcatcgat
     3361 tatgtcgggg tgatgcgcgc ggcttgggcc aatctcgtag cgggcgggcg gcgacagggc
     3421 gcgagcacca ttacgatgca ggtggcgcgc aacttcttcc tgtcttcgga gaagacgctt
     3481 acacgcaagc tctacgaagc gctgctcgcc ttcaagatcg agcacagcct cacgaaagag
     3541 cagatcctcg agctctatgt aaatcagatc tacctgggac atcgggctta cggcttcgcc
     3601 gccgcctcac agatctacta cggcaaggcg ctcggccagc tttccctcgc cgagaccgcg
     3661 atgctcgcag gcctgcccaa ggcgccgtcg ctctacaacc ctatctctaa tgcgcagcgc
     3721 gccaaggtgc gccagcagta cgtgctacgg cgcatgacgg agctcggtta catcgacgcc
     3781 acgcagtacg aggacgcgct caaggcgccg gtgcgtaccc gacgcgaagt tacggagtac
     3841 tcggtgcatg ccgagtacgt ggccgagatg gtgcgtcagg cgctcgccga gcactacccg
     3901 gaggacgtct acacgcgggg tttccgcgtg tacaccacga ttcgcaaggc cgaccaggag
     3961 gccgcttatg aagcgctgcg ccgcggcgtg ctcgagtacg accggcgtgc cgggtatcgc
     4021 ggtcccgagg gctatgtcga gctgccagcc aaccccaatg acgacgacat cgaggacgcg
     4081 ctggcggacc gacctgacaa cgacgacctg atcgccgccg tcgtgctcgc ccatgacggc
     4141 aagcaggtgc aggccgggct gcgcaccggc gagcgcatct ccatccaggg agcgggcttg
     4201 cagttcgccg cgcgcgccct tgacgccaag gcgccaccgc aacggcgcat ccgcaagggc
     4261 gcgatcgtgc gcgtgcagcg cgacggcaag aactggcaga ttgcgcagct tccggaagtc
     4321 gaggccgcct tcatcggcat cgacccgcag gacggcgcgg tgcgcgcgct ggtcggcggc
     4381 ttcgacttca gccgcaacaa gttcaaccat gtgacgcagg cttggcgtca gccggggtcg
     4441 tcgttcaagc ctttcatcta ctcggcggcg ctcgaaaaag gattcaccgc cgcgaccgtc
     4501 gtggcagatg aaccagtggt gctcgaagcc gagcagaccg gaagccagcg ctgggagccg
     4561 aagaattacg acggcaagtt cgagggcccg atgcgccttc ggaccgcgct tgccaagtcg
     4621 aagaacatgg tttcgattcg cgtcctcgac gccattggcc cgaagtacgc ccaggattac
     4681 gtcacgcggt tcggcttcga agccgagcgt catccggcct acctgacgat ggcgctcggt
     4741 gcgggctccg tcaccgcgtg gcagatggcg cgcgcctatg cggtctttgc gaacggtggt
     4801 ttcctggtgc agccgtattt catccacaag atcgtcgacg atcgcggcaa cgcgctggcg
     4861 gtcgccgaac cgcggcgcgc gggtgacgaa gtgctgcgcg tgctcgaccc gcgcaacgcg
     4921 ttcatcatgg accacatgat gcaggacgtg acgcgcgccg gaactgcggc gcgtgccgcg
     4981 cggcttggcc gcagcgacct cgcgggcaag accggcacga cgaacgagtt cgtcgacgct
     5041 tggttcgcgg gctaccagcc ggcgttggtc ggcatatcgt gggtggggta cgaccagccg
     5101 cgcacgctgg gcaagaacca gacgggcggc gtggtcgcgc tgccgatctg gcttggctac
     5161 atggagcgcg tgctgtccga ctacccggag atggcgcgca ccatgccgcc gggcgtggtt
     5221 gccgtgccga ccatggcgct ctccggcgag ccgaagatga ttccggagtt tttctaccgg
     5281 gaggccatac ccccgcccga tgtgttgacg ccacccccgc caccaacggc gccgacgccg
     5341 ctacccgagc agtcgaaccc cccggcctag ctcacgggag agcagcgccg aaagcgcagc
     5401 gaagaactct tcgccgctcc gtgtatcgcg ccatgcggag ccgtcccagc ggaagtgaaa
     5461 gccgcccgag cgagcggcaa cccagagctc gcgcgcggcg gagtgtcggt tgacgatcat
     5521 cttcgcgccg tcgtcgaact cgatctccaa aacgccacct tccttacgct cgaagtcgag
     5581 ctcgaggccc tcgagcgcat gctcgagccg ctccagcgct tcctcggcag ccacttcgaa
     5641 tcgatggtcg tccatgcgtt aacatgcgga ttttatgcgc ttcgttgtgc tcctcgccgc
     5701 ctgcgcgctg gccgccggct gcggtcagaa ggggccgctt ttcctgcggg agagcccgcc
     5761 gccgggggtg aagccaccca agccgaagcc ctacgagccg gttccatacc ctcccgagct
     5821 caccgaagaa gagaagaagt aaggcgagct tgcctttcaa ctaccgccat agcgtgctct
     5881 gcgccgacga ggtggcgctc gacgaaatcg ccgagcgctt cggcacgccg tgcttcgtct
     5941 attccgcggc ctccattgcc gcgtcctatg ccgagttcga agacgctcta aagggccgcg
     6001 cggcccgcat ctgttactcc gtcaaggcga attcgaacct tgcggttctc gccttgctcg
     6061 cgaagctcgg ggccgggttt gacatcgttt ccggcggcga gctggcgcgg gtgctggcag
     6121 ccggcgggga tgcgcgcagc acgcttttct ccggggtggg caagagcgaa gccgaaatct
     6181 tttttgccct caagaagggt atcggctgca tcaacatcga atcggaggcc gagctcgacc
     6241 gcgtccaggc ggtagcggcg tcgctccggc tgcgtgcccc ggtggcgcta cgcgtcaatc
     6301 cggatatcga tgcgaacact cacccctaca tctctaccgg gctcagtcat acgaagttcg
     6361 gcgtgccgca cgcggaggcc gagagcctat accgcaaggc ggccgatatg ccgaacatcg
     6421 acgtcgtcgg catcggctgc cacatcggtt cccttctgct cgaagccgcg cctttcatag
     6481 cggcgaccga gcgattgctc ttgctcgtcg atcggctcga ctcggccggc atccggctgc
     6541 ggcatgtaga tgtgggcggc ggcatcggca ttcgctacaa ggacgaatcg ccgcagccgc
     6601 cggaggcctt cgtgcgcgga gtgctcgccg cgctcggccg gcggccgcac acggtcatct
     6661 tcgaccccgg tcgctccatc gtagggaacg ccgggctgct cctcacccga gtggagtact
     6721 taaagcccgg gttcctcgtc gtcgacgccg ggatgaacga cctgatccgg ccggccctgt
     6781 acggtgcctg gcacgaggtg cgggcggtgc gggaatccgc ttccactacg ggcgtttacg
     6841 acattgtcgg accggtgtgc gaaagcgcgg acttcctcgc caaagggcga cccctagcgg
     6901 cccgcgcagg cgaccttctc gctgtgatgt cctgcggcgc ctatgccatg gcaatgagct
     6961 cgaactacaa cacccggccg cgcgcccctg aagtactcgt ccgcgatggg cgtgcgctat
     7021 tggtgcgacg ccgcgaatcg gtcgaagagt tgtttgccct cgagcgccgt ccggactgac
     7081 aatcgcccgt tccatgacca ccgccacgcg ccgtttgacc ctgctcggga tctgcgtcgc
     7141 cctgatcgca ggcgccgtcg cggcgtattt ctacgccgcc gacgggcgtg cggcgcgcaa
     7201 ggagatcgcc aaaaccgcgg ccaaaggggc gggccaggcc gtgctaatta cgacggcgcc
     7261 ggtgacgccg cgcacgctgg agatctatga ggaagtgctc ggcacgatcg agaatgtaat
     7321 cgatccgacg atcggcgccg aggtcgccgg ccgcgtcaca cgggtcaccg ggttcaccgg
     7381 taggaaggtg aaaaagggcg agctcatcgc cgagatcgac gcggccgact tccagattca
     7441 gcagcgcgcc gaccaggccg agatcggccg cttgacgagt ctgctcgagc agcaggaccg
     7501 cgtcgtcgaa cgccagcaga agctcgtcgc gcaaggcttc atctcgaaga acgcgatcga
     7561 tgacgcggtc gcccagcgca acgcgctgcg ccaatcgctc gccgccgcgc gcgcccgctc
     7621 cgacgccact ggccgcagcg tgacgaaggc gcgcgtggtc gcgccggtgg acggcgagat
     7681 cgagacgcag atcgtcgcca tcggtgacta cgtgaagctc ggtgatccgc tgtttcgcat
     7741 ggtgggggtg caggaactgc gcgtgcacct gctcctgccg gaagccgcgg ccacgcgcat
     7801 caaaccggga ctcaaggtgg tgttatctcc ccccgccgca cccgagcgcg tactggaggc
     7861 ccggatcgac gaaatcaagc cgacggtggg caccaacaac cgcgcgctcg atgcgatcgt
     7921 gaagatccgc ggcgagggca cgctattgcg cggcggcggc acggtcaacg ctcgaatcgt
     7981 caccagttcg ctggacggcg cgctgatggt gcccgagcag agcgtggtgc tgcgtccggc
     8041 cggcaaggtg gtctacgcga tcgaaggcag cgtcgcgcgc cagcgcgtgg tcgaaacggg
     8101 gttgcgccag gacggttggc aacaggtcgt gaagggcctg tcggccggcg agacggtggc
     8161 cgccgacggt gccggcttcc tcaccgacgg cgccgccgtc acggtgcgcg cgaagccggg
     8221 cgcggttgcc gaagaaggca cgaagaaggg cggctcatga cgctctccga gctctcgatc
     8281 cgccggcacg tgttcgcgtt catgctcagc gcggtactgg tgctgttcgg cgcgatcgcc
     8341 taccagcgca tgggcatcga caagctgccc tacatcgagt ttccggtgat ctcaatcacc
     8401 accgcgcagc gcggcgcgaa tccggacatc gtcgatgcgt cgatcaccaa tctgatcgag
     8461 accgcggtca acagcgtccc cggcatcgag catatccagt cgacatcctc gccgggcacc
     8521 tcggtcgtcg tgatcacgtt cgggctggag aagcgcatcg atgtcgcctt tcaggaggtg
     8581 caggccaagg tgaatcaggt ggtgcgccgg ctgccgaagg acgccgaccc gcccgtggtg
     8641 gccaaggtcg agacgaacac tcaacccatt ctgtggaccg cgctgcaagg cgaccgcacg
     8701 cagcagcaac tcaaccagta cgcgatcaac gtcatcaaga agcagctcga aacgatcgac
     8761 ggcgtgggcg agatccgcat cggcgggcgc cgcgaccgcg tgattcgcgt cagcctgctg
     8821 cccgcgaagc tcgccgccta tggcatcgcc gcccaagaca tctcggatgc tttcggcaag
     8881 gagcatctgc agctggccgg cgggttcctg gtcggtcgtt cgaccgagca tctgctcaag
     8941 ctcgacctcg agttccacaa gctcgacgac cttgccgcga tgatcgtcgg ctacaaggat
     9001 ggcgcgccgg tgcgcctgcg cgacatcgcc gaggtggagg acggcctggc ggacttccgg
     9061 caactggcgc gcttcaacgg caagccggcg gtcggcatcg gcgtagttaa ggtgccgaac
     9121 accaataccg tcgcgatcat cgacaaggtg cttgagcgca tcgaaaagga cgtgcgcccg
     9181 cagcttccgc ccgggctcga gctgcactac gtgcagaacg acaaggtgtt catcggcgca
     9241 atggtggagt cgctgaagca gcacctcgtc gagggcacta ttctcgccgc gctcatcgtg
     9301 tggctcttcc tcaagagctt ccgctcgacc gtgatcattg cctgctcgat tccggtatcg
     9361 ctcatgggcg cgatcgcggt catgtatttc ttcggctaca ccttcaactc ggtgaccctg
     9421 ctcgcgcttc tgctgctggt cggcgtagtg gtggacgact cgatcgtggt gctcgagaac
     9481 atctaccgcc atcgcgagga ggaccgcgaa accagcgcct tggcggcggc ggtgacgggc
     9541 agccgcgaag tgacctttgc ggtcatcgcg gccagcttct cgctcgtggc gatctttgcg
     9601 ccggtggtgt tcgtgagcgg catcctcggc cagttcctgc gctcgttcgc cgtggtggtg
     9661 accttcggcg tgctggtgtc gctgttcgtc tcgctcaccc tcacgccgat gctgtgctcg
     9721 cgctacctgg atgtgaagga caagcacggc cgcgtgtact gggtgctcga gcgtttcttc
     9781 catggcctgg acggcctgta ccggcgcctg ctcgcctggt cgctgcgcaa ccggggcttg
     9841 gtgcttggcg gcgcgatgct gtgcttcgtg gcctcgcttc cggtgttcat gcagctgccg
     9901 cgcgagctcg cgccgcaggc cgacgagggc cgcttcctgg ttggcatccg cacgccgctt
     9961 ggctcctcca tcgagtacac cgagtcgaaa ctacgcgagg tagagcagat cgcgttccgc
    10021 tatcccgaga tcgtcaccga gttcggcgtg atcggtctgg gctcggcgca gcaggtgaac
    10081 caggccaccc tggtcgcgcg catgaagccg cgcgccgagc gccagcgctc tcaggccgag
    10141 gtcatcaacg ccattcggcg cgacctgcag caggtggccg gcgcacgcgc tttcccgcgc
    10201 gctttcgggc tggtgcaagg ccagcgctcc gagccattgc agtttgtcgt gaaggggccg
    10261 aacctgcagg aaatggggcg gcttgccgcg gagctgcagc gcacgctgca ggccgacccg
    10321 cagatcgggc gcatggatac ggacctgcag ctcgacttgc cgcagctcgt gctcgagccc
    10381 gaccggctgc gcgcggccgg cttgaacctc acctcgggcg acgtcgcgct cgcggtcagc
    10441 atgctgaccg gcgggatcga catcgccaag tacaacgact atcccggcga cggcgagcgc
    10501 tacgacatcc gagtgaaggc gaaggacgga gagttccagc agcaggcgga cctcgccaag
    10561 atctacttgc gcagccgcca ggggcagctc gtgcgcctgg accaggtggc gcgttttcgc
    10621 gaggcgctcg ggcccgcggt gatcgcgcgt ttcgacctgc agtattcggc gagcttttac
    10681 gcctcgccga cgatcgcgct cggcgccgca atggagaaag tgcgcgcagc cgcgcaaaac
    10741 ctgccgatcg gctatcgcgt ggtgttcgtc ggcgaagccg agcagctcga gaaaaccgct
    10801 aacgcgacgc agttcacctt cgcgctcgcc cttgcgctgc tcttcatggt gctcgccagc
    10861 cagttcaaca gctttttgca gccgttctac atcatgctgg cggtgccgct cggcatcgtg
    10921 ggcggcatcg ccgcgctctg gctttccggg agcaccctca atatctactc cggaatcggc
    10981 ctggtgctcc tcatcggcct cgtggcgaag aactcgatcc tgctggtcga ccttaccaac
    11041 cagcggcgcg agcaaggcct ggcggtcgac gaggcgctgc gcgacgcctg cccgacgcgc
    11101 atgcgtccgg tcgtgatgac ctcgctcacg atcattctcg cgctcgcacc cgcggcaatc
    11161 ggtctgggcg ccggcgcgga gactaacgcg ccgctttcga tcgccgtgat cggcggcatg
    11221 attacctcga ccctcctcac gctcatcgtc gtgccggcgg ggtattctct cgttgagaac
    11281 tggcgtcagc gccgcagcgc cgctcgcttc gcctccggca cggctgatta gcgtggcgag
    11341 cccggacgcg cgcagtctcg ccgttttctg tgacttcgag aacatcgctc tcggcgtgcg
    11401 cgaggcgcgc tattcgcagt tcgacatgga gaaagttctc gagcgcctgc tcgccaaggg
    11461 ctcgatcgtg gtgaagaagg cgtactgcga ttgggagcgc tacaaggagt tcaaaagcac
    11521 gatgcacgcg gcgtcgttcg agctgatcga gatcccgcac gtgcgccaat cgggcaagaa
    11581 ctcggccgac atccgcatgg tggtcgacgc gctcgatctc tgctacacga agtcgcacgt
    11641 cgacaccttc gtcatcgtga gcggcgactc cgacttctcg ccgctcgtct caaagctgcg
    11701 cgagaacgcg aagacggtga tcggcgtggg cgtgaagaac tcgagctcgg acctgctcat
    11761 caacaattgc gacgagttta tctattacga cgacctcgtg cgcgccgatg agcagaaacg
    11821 ccgcgcagcg aagaagcggc gcgaagccgc gcccgcgggg gcgagcggcg acaagagcaa
    11881 cggcgacaag aagcaggagg ccttcgacct gatgctcagc acgctcgaag ctctgatggc
    11941 cgagcgtgac gcggacgagc gcatctgggg ctcgatggtt aagcaggcgc tgaagcgccg
    12001 aaacccggga ttcaacgagt cgtactatgg cttccgtgcc ttctccgacc tcctcgagga
    12061 agccgaaaag aaaggcctca tcaagctcga agccgacgag aagtccggcg gctacgtggt
    12121 caggcccgcg cagtaaggcc gaactgcagg cggcggcggg caggggcctg cgcgacgtca
    12181 tcggtccccg cctgaaggtg cttttctgcg gcatcaatcc ggggctctat tcggcggcga
    12241 ccggctttca tttcgcgcgg cccggcaacc gcttctggcc cgcgctgcac gcggcgggat
    12301 tcaccccgcg ccttctccac ccttccgaga acgagcggct gctcgagctc ggctacgggc
    12361 tgaccaatct cgtaaaccgc ggcaccgccg ccgcggacga gctttcgacc ggggaattga
    12421 tccggggagg ccgggggctc gcggcgaagg tgcggcgctt ccgtccacgc gccgtggcgt
    12481 ttgtcggcct cggcgcctac cgcgtggcct tcggtcgtcc gcgggcgacg ctcggcctgc
    12541 agcccgagcc cttggaaggc gccgacgtgt gggtgctgcc cagtccgagc ggcctgaacg
    12601 cgaactacca gttgcccgcg ctcgtcaaac tgtttcgtgc cctgcgcatg cggagctgac
    12661 catggccacc aaactggaca agtccttaaa gcgtgagatt gagatcgacg gccaggccta
    12721 catgctgacc atcgacccgg ccggcatgaa gctcgtgccg aagggcaagc gcaacgggct
    12781 cgagctcgcc tggaaagcat tgatcagcgg cgacgccgcg ctcgctagcg cgctcaatgc
    12841 ctctctcaaa gtctgacgac ctccacggcg ccgcgcccga caagtgcgcg gtcgcgctcg
    12901 tcctcatcga ctggatcaac gatctggaat tcgattcggg cgccaagctc ctgcccgaag
    12961 cgctgaaggc ggcgaaggcg accgcggcgc tgcgcaagcg cgccaagcag gccggcgtgc
    13021 ccgtcatcta ctgcaacgac aacttcggca agtggcgctc ggatttccgc tcgctgctgg
    13081 aacactgcct gaagggtgag gtgcgtgggc gcgcgatcgc cgagctgctc gcacccgatg
    13141 aagaggacta ttttgtgctg aagcccaagc actcgggctt ccattcgacg acgctcgacg
    13201 tgctgctcgc ccacctcggc agccagacgc tcgtcctcac aggaatcgcc ggcaacttct
    13261 gcgtgctgtt caccgcgcag gatgcctaca tgcgcgactt caagctcgtc gtgccgcgcg
    13321 actgctgcgc ctccgaggaa gaatcggaca atcgccacgc gctcgagcac atggcgaagg
    13381 tgtgcaaggc cgacacccgg cgctcaaccg acatcgattt caacagcctg cgcaaggctg
    13441 cttaaggcgg gttgagctgc tggtaagccg cgttcgcgac gttgaggagg tcttccttgc
    13501 tgatgtcggc cgatgaaacg gcgtagccga tcttcgtgtc gacccagtag aacacaccca
    13561 cattggcttc gcgcgcatag cgaaacgcgg tcgagcggtt cgagcccatg tcgctacgca
    13621 cgtaaagcgt gacacgcgtg ccgcggttgc ac
//
LOCUS       DASTPD010000351         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1828799, whole genome shotgun sequence.
ACCESSION   DASTPD010000351 DASTPD010000000
VERSION     DASTPD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828799"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFR57_11885"
     CDS             complement(<1..721)
                     /locus_tag="VFR57_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0260101.1"
                     /translation="MLTGRGRYVSDWNLAGQAYAYFLRSDRAHAEIVSIQKDEALGAK
                     GVIAIFTGDDLDLKSLPAALPAKGRGGMELINPGRPALAKGRVRFTGDAVALIVAETA
                     AAAQDAAELVAVGYRDLPAVTDATEALASGAPLVHDSVPGNMVLDYESGDEAKTKEAF
                     AKAARIVRLDVDISRVVGNPMEPRACLAAFVSDQYHLYTCTQGALIMRNQLSVVLGVP
                     PERIRVIAEEVGGGFGVRFNLY"
     gene            complement(781..1221)
                     /locus_tag="VFR57_11890"
     CDS             complement(781..1221)
                     /locus_tag="VFR57_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0260102.1"
                     /translation="MKYRKILLAYNGSQEGKRALLECADLAGFLQAETHLLAVASMPP
                     SLFLTEGFVPEELLEEEKKRTQAVLDEGIRTLRERGFNATGQLAVGEPVEEICRLAKT
                     RGVDLIVIGHNQNTSFAARWWKGSVGASLLDYAPCSILIAMSKG"
     gene            complement(1231..2175)
                     /locus_tag="VFR57_11895"
     CDS             complement(1231..2175)
                     /locus_tag="VFR57_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0260103.1"
                     /translation="MRKLLALAGSLLLAAVAHAQQFPSKPVTLIVPWPAGGSTDIYFR
                     KLGEITARHLGQPLVIENRPGGSGMNGPATMAKTARPDGYTISQLAITAFRVPHMQKV
                     DWDPITDFTYIVGLAGYTFGIVVKADSPFKTFQDLLAYARANPGRLSYATPGTGTSLH
                     LAMEEIAAKAGVQFLHVPFKGYGDGAIALMGGHVMVQVDSTGWAKQVDAGAQRLLATL
                     GDKRTRWNAPTVKELGVDTVSNSPFGLVGPKGMPREVVKVLHDAFKKSLDDPEYLKML
                     AQLDQPAWYMSSEDYGRWAVDMLKAERATIERVGLLLK"
     gene            2233..3726
                     /locus_tag="VFR57_11900"
     CDS             2233..3726
                     /locus_tag="VFR57_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU0260104.1"
                     /translation="MLDPNRSDLVAGVAGAGTMGRGIAQVLAQCGARTLLYDAQPGAA
                     RKARDSIAQALGKLVEKGRLGAGVADNAVGRIEIIDSVDAFGPSHVVVEAIVEDLNAK
                     QQLFSSLEKQVGSDCILASNTSSLSVTAMAAACQRPGRVAGYHFFNPVPVMKIVEVVD
                     GVLTEPWVTDALAALARRFGHTPVRCKDTPGFVVNHAGRAFVPEALRVLSEGIADFAT
                     IDRILVDAAGFRLGPFGLMDLVGLDVAHSVMKSMYRQYYEEPKYRPSFLAEPRVAAGL
                     LGRKTGRGWYEYGQEGPKAIAEASPPKIKPHAVWAVPELKELLFELGAKPELKPTPEA
                     VCFVAPLGTDATTSALELGLDPRRTVAVDPLFGFSKRRTLMRTCITGKEILEAAHGLL
                     AADGVPVSVINDSPGFVAQRVVAHIVNVGCDIAQMRIACPEDLDRAVTLGLGYPRGPL
                     AMGDALGPKRVLAVLEAMHDFYQDPRYRPSPWLRRRAALGVSLLKEE"
     gene            3740..>3869
                     /locus_tag="VFR57_11905"
     CDS             3740..>3869
                     /locus_tag="VFR57_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140074.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU0260105.1"
                     /translation="MLDAYIYDGLRTPIGRHAGKLAPVRPDDLAAEVLREVVARNQI"
BASE COUNT          660 a         1288 c         1255 g          666 t
ORIGIN      
        1 ggtacaagtt gaagcgcacg ccgaagccac cacccacctc ctcggcgatc acgcggatcc
       61 tctcgggcgg cacgccgagc accacggaga gttggttgcg catgatcagc gcgccttgcg
      121 tgcacgtata gaggtggtac tggtccgaaa cgaacgcggc aaggcaggcg cgcggttcca
      181 tgggattgcc gaccacgcgc gaaatgtcga cgtcgagccg tacgatccgc gctgccttgg
      241 cgaaggcttc cttggtcttt gcctcgtctc ccgactcgta gtcgaggacc atgttgccgg
      301 gcaccgagtc gtggacgagc ggcgcgccgg acgcgagcgc ctcggtggcg tcggtgacgg
      361 cggggaggtc gcggtacccg accgcgacca gctcggccgc atcctgcgca gccgcagcgg
      421 tctcggcgac gatgagcgcc acggcatcgc cggtaaagcg cacgcgcccc ttggcgagcg
      481 ctggtcttcc ggggttgatg agctccatgc cgccgcggcc cttggccggg agcgcggccg
      541 gcagcgactt gagatccagg tcatcgccgg tgaagatggc gatgacgcct ttcgcgccta
      601 gcgcttcgtc cttttgaatc gaaacgatct cggcatgagc gcggtcggaa cgcagaaagt
      661 acgcgtacgc ctggcctgcg aggttccagt cggagacgta gcgcccccga ccggtgagca
      721 ttcggtcatc ttcgagcctg cctttcagca tgcccgaatt gtattaaatc gccgctcggt
      781 tcatcccttc gacatcgcga tcaggatgct gcagggcgcg tagtcgagca gcgacgctcc
      841 cacagagcct ttccaccagc gcgccgcaaa cgatgtgttc tggttgtggc cgataacaat
      901 caggtccacg ccccgcgtct tggcaaggcg gcagatctcc tcgaccggct cgcccacggc
      961 gagctggccg gtggcgttga agccgcgctc gcgcagcgtg cggatgccct cgtcgaggac
     1021 cgcctgcgtg cgcttctttt cctcctcgag cagctcctcg ggcacgaagc cctcggtgag
     1081 gaagaggctc ggcggcatgc tcgcgaccgc gagcaggtgc gtctctgcct ggaggaagcc
     1141 ggcgagatcg gcgcactcga gcagagcgcg cttgccttcc tgcgatccgt tgtaggcaag
     1201 aagaatcttc cgatacttca tggcgcctcc ctacttgaga agcaatccca cgcgctcgat
     1261 ggtggcgcgc tcggccttca gcatgtcgac ggcccagcgg ccgtaatcct cgctcgacat
     1321 gtaccaggca ggctgatcca gctgcgccag catcttgagg tactcgggat cgtccagcga
     1381 ctttttgaac gcatcgtgca gcaccttcac cacctcgcgc ggcatgccct tgggccccac
     1441 caggccgaac ggcgagttcg acacggtatc gacgccgagc tccttgaccg tgggcgcatt
     1501 ccagcgcgtg cgcttgtcgc cgagcgtcgc gagcagccgc tgcgccccgg cgtccacctg
     1561 tttcgcccag ccggtggaat cgacctgcac catcacgtgc ccgcccatca gcgcgatcgc
     1621 gccgtcgccg tagcccttga acgggacgtg gaggaactgc acgccggcct tggccgcgat
     1681 ctcctccatg gcgagatgga gcgaagtacc ggttcccggc gtggcgtagg aaagccggcc
     1741 ggggttcgcg cgcgcgtacg caagaagatc ctggaaggtc ttaaacggcg agtcggcttt
     1801 caccacgatg ccgaaggtgt acccagcaag gccgacgatg taggtgaaat ccgtgatcgg
     1861 gtcccagtcg accttctgca tgtgcggcac gcggaaggcc gtgatggcga gctgcgagat
     1921 ggtgtagccg tcgggccgcg cggtcttcgc catggtcgcc ggcccattca ttccgctgcc
     1981 gcccggccgg ttctcgataa cgagcggctg acccaggtgt cgggcagtta tctcgccgag
     2041 cttccgaaag tagatgtcgg tgctgccacc cgccggccac ggaacaatca gtgtgacggg
     2101 tttcgacggg aattgctgcg cgtgcgcaac agccgccagc agcaggcttc ccgccagggc
     2161 cagtagcttt ctcatgctcg cctcctcctc gcggcgatta tagccgccac ctccggccgc
     2221 tagaataagg gcttgctaga tcccaaccgc agcgatctcg tcgccggcgt cgccggtgcg
     2281 ggcaccatgg gccgcggcat tgcacaggtt ctcgcccagt gcggcgcccg gacgctgctc
     2341 tatgacgccc agccgggcgc agcgcgcaag gcgagagact cgatcgccca ggcgctcggc
     2401 aagctcgtcg agaaagggcg gctcggcgcc ggggtggcgg acaacgccgt cggccggata
     2461 gaaatcatcg actcggtgga cgcgttcggg cccagccacg tcgtggtgga agccatcgtc
     2521 gaagacctga acgcgaagca gcagctcttc tccagcctcg aaaagcaagt gggaagcgac
     2581 tgcattctcg cctccaacac ttcctcgctc tccgtcaccg cgatggcagc tgcgtgccag
     2641 cggcccggcc gcgtcgccgg ctaccacttt ttcaacccgg taccggtaat gaagatcgtg
     2701 gaggtggtcg acggagtcct caccgagccg tgggtaaccg acgcgctcgc cgcgcttgcc
     2761 cgccgcttcg gccacacgcc ggtgcgctgc aaggacacgc cgggattcgt cgtcaaccac
     2821 gccgggcggg cattcgtgcc cgaggcgctg cgcgtgctgt ccgagggcat cgccgatttc
     2881 gcaacgattg atcgcatcct cgtcgacgca gcgggattcc ggctcggacc tttcgggctc
     2941 atggacctgg taggccttga tgtcgcgcac tccgtcatga agtcgatgta ccggcagtac
     3001 tacgaggagc cgaagtaccg cccctccttc ctcgccgaac cgcgcgtcgc tgccggattg
     3061 ctcggacgca agacggggcg aggatggtat gagtatggac aagaagggcc aaaagcgatt
     3121 gctgaagctt ccccaccgaa aatcaagccg cacgccgtct gggcggtgcc ggagctgaag
     3181 gaattgctgt tcgagctcgg ggcgaagccg gaattgaagc cgacgcccga ggcggtctgc
     3241 ttcgtggcgc cgcttggtac cgacgcgacc acgagcgcgc tcgagctcgg actcgaccca
     3301 cggcgcacgg tagcggtaga tcccctgttc ggcttctcca agcgccgcac cttgatgaga
     3361 acctgcatta caggaaaaga aatcctggag gcggcgcacg ggcttctcgc tgccgacggc
     3421 gtgccggtgt cggtgatcaa cgactcgccc ggcttcgtcg cgcagcgcgt ggtcgcgcat
     3481 atcgtgaacg tcggctgcga cattgcgcag atgcgcatcg cctgcccgga agacctcgac
     3541 cgcgccgtca cactcgggct cggctatcca cgcggtccgc tcgccatggg cgacgcgctc
     3601 gggccgaaac gggtgctcgc ggtgctcgaa gcgatgcacg atttctacca ggacccgcgc
     3661 tatcgcccca gcccgtggct gcgcagacgc gccgcgctcg gggtctcttt attgaaggaa
     3721 gaatagagaa agccacccca tgctagatgc ctacatttac gacggactgc gcacgcccat
     3781 cggccggcac gccggcaagc ttgcgcctgt gcgccctgat gatctcgctg ccgaagtgct
     3841 gcgggaggta gtagcgcgta accagatca
//
LOCUS       DASTPD010000352         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_808276, whole genome shotgun sequence.
ACCESSION   DASTPD010000352 DASTPD010000000
VERSION     DASTPD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808276"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..792)
                     /locus_tag="VFR57_11910"
     CDS             complement(37..792)
                     /locus_tag="VFR57_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0260106.1"
                     /translation="MTSILQVQRLTKRFGGLTAVSELGFELYEGEILGLIGPNGAGKS
                     TTFNLIAGAYASDAGEIQFLGKSLSGLRPHQIARRGIMRTFQHNRPFDGLSVLENVLV
                     GAHTRFRRNPFSRKASLEETEQCRRAEELVRFVGLGELRDAPVERLSFGQGRLLEIAR
                     ALAGEPKLVLLDEPAAGLTHAEAERIAAVIRGIAARRIAVLLIEHDMHFLLPLAHRVV
                     VLNFGAKIAEGTCEQVRSHPAVVDAYLGDAAAL"
     gene            complement(758..1705)
                     /locus_tag="VFR57_11915"
     CDS             complement(758..1705)
                     /locus_tag="VFR57_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0260107.1"
                     /translation="MNTKNATALAGWVAAALVMSAAAFFLSSYYNDVYRKLLLWIALA
                     LSYNFLFGISGQVAFSHFVFYGIGAYAVVIFLFQLGMPLPLAILAGVLVCAAIALAVA
                     IPSTRLEGFYLALATLALAQLFIVVLNEGGAVTGGTGGLVDYRLPPVFGVRITGPWYT
                     VVIVVLVLATYAVLLRLDRSWFGRACRAVRDNPEAAAAMGIDVARTKVTAFTLTSVLA
                     GVAGMAYAFVDNTVNPPIFGLENAFLLLFMVIVGGAGRHLGAIIGAVLLYLLPFLLSP
                     LIGHHHALVFGVLMVAAILLQPRGLVGIYDKYSSGPKAH"
     gene            complement(1702..2547)
                     /locus_tag="VFR57_11920"
     CDS             complement(1702..2547)
                     /locus_tag="VFR57_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0260108.1"
                     /translation="MQDLLELSISATASGCIYALVALAYLLMIRPTGIINFAVGEWAL
                     VGAFGGFLLLSRFEWPYPLGMAAILGFMLLVGWATERITVRPLVERGAPLLAPILALL
                     GMLVVFRESISLGFGPDPHQVPEPVSWEYLKYFMIGTTLLVFAAVWLFFERTVWGKSF
                     EAVALNRKAAALVGIDLARVTAASFAGGAAAAGLAGLLVAPNVSAHYLMGVPLAIQGF
                     TALVIGGVGRVEGALLGGLLVAFVEQLTVRYAPLPAGYAQGVPLVLLMLFLLVRPTGL
                     LRARG"
     gene            complement(2562..3689)
                     /locus_tag="VFR57_11925"
     CDS             complement(2562..3689)
                     /locus_tag="VFR57_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260109.1"
                     /translation="MRKLFAVALALFAASVFSQETIRIGVITDKVGPAKPYSEPTLEG
                     ILFGADEINKAGGLLGRKIELLIEDDQGRPDISAAMARKLVDAGAVFILSITLTPATQ
                     QQQNVTLEAKTPQMTPMNSGDTLTTQLPNPYFWQTGPLGSVQIATLLSHARAKQLKRV
                     ALISDNSDLGQLLAKFFRAGLEKSGIQVVTEEVVPRGATSAEPQMQKIRAANPDALFM
                     AGVLTPENVLILRAYRQMGLKFPIHSSYNLSVPQYMSVAKGLLNGITFVDAYDPDKPE
                     VQKFEADYQKATGKQPYNIHGYGYDGIRMVAEAIRRAGSTDKEKIRAAMQSLTFSGVM
                     GAKGMKYVFPEGKRIGFDPNGMVVRVYEGDKQGRVVHVGQK"
     gene            complement(3703..4857)
                     /locus_tag="VFR57_11930"
     CDS             complement(3703..4857)
                     /locus_tag="VFR57_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0260110.1"
                     /translation="MKFSLAPEWVHVQYKEAAKFTEVYGFAGTQGAVNCEGVRFLPKG
                     RPSKTLAVFMHPSSTLQLLPMPQALARAGVHVLCAGSRFAKNDTPLIMEKVLLDLGAY
                     IRRAKEVWGYDRVVLCGWSGGGSLALFYQSQAEKPSITETPAGDPVELKALIPADAMI
                     FQAAHLSRARMLSEWIDPSVTDEANPDERNLELDIYDPRCPNQPPYSKDFVETFRSAQ
                     LRRMRRRTGWVREALRKLKGDEMERGFVTYRTMCDVRFMDPALDPNDRKPRWCYLGKP
                     ETANTGPVGIGRFSTLRAWLSQWSIDDSNADGPKCARSISVPLLAIENSADDAVPQPH
                     ARIIHDAAGSRDKAFHVIQGATHYYQGQPELLEQAVELCVGWMARRGLLE"
     gene            complement(4867..5391)
                     /locus_tag="VFR57_11935"
     CDS             complement(4867..5391)
                     /locus_tag="VFR57_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0260111.1"
                     /translation="MDIKSLHHVAYRCKDAKETADFYTRVLGLEYAMAMAEDHVPSTG
                     EYSPYMHIFFRMADGSYVAFFEVPESPPMGRDQNTPLWVQHLALRVPDEKALMAYKAK
                     LEKLGIEVVGPTDHTICKSIYFFDPNGHRLELAVDTTTPDMKKRLAAVSDEMLQEWSK
                     TKKAPHHADWVHKK"
     gene            5420..5800
                     /locus_tag="VFR57_11940"
     CDS             5420..5800
                     /locus_tag="VFR57_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0260112.1"
                     /translation="MDKRNHQERHDAGLKTRREVLGPAYVDKAMAGVDDFSREFVELL
                     NTYCWNDIWNRPGLPRKTRSILNLGMLCALGKEHELKLHIRGALNNGLSKEEIKEVFL
                     QVAIYCGVPAAVVAFRCAKEVFQS"
BASE COUNT         1087 a         1876 c         1888 g          980 t
ORIGIN      
        1 tggagcaccg gcgccgcgcc gtaggaggcg cgcagctcag agagccgcag catcgccgag
       61 gtaggcgtcg accaccgccg gatgcgagcg cacctgctcg caagtgccct cggcgatctt
      121 ggcgccgaaa ttcagcacca ccacgcggtg cgcgagcggc agcaggaaat gcatgtcgtg
      181 ctcgatcagc agcaccgcga tgcggcgcgc cgcgatgccg cggatcacgg cggcgatgcg
      241 ttcggcctcg gcatgcgtca gccccgccgc cggctcgtcg aggagcacga gcttcggctc
      301 gccggcaagc gcgcgcgcga tctccaggag ccgcccctgg ccgaatgaga gccgctcgac
      361 gggcgcatcg cgcagctcgc cgagaccgac aaacctcacc agctcctcag cacgacggca
      421 ctgctcggtt tcctccaagg aagctttcct ggagaacggg ttccttcgga agcgcgtatg
      481 ggcgccgacc agcacgttct cgagcacgct gaggccatcg aacggccggt tgtgctggaa
      541 ggtgcgcatg atgccgcggc gcgcgatctg gtgtggccga aggccactca atgatttccc
      601 caagaactgt atttctcctg catccgacgc gtacgcgccc gcgataaggt tgaaggtggt
      661 gctcttcccg gcgccattcg gcccgataag cccgaggatc tcgccttcgt agagttcgaa
      721 gccgagctcg gacacggccg tgaggccgcc gaagcgctta gtgagccttt ggacctgaag
      781 aatacttgtc atagatgccg accaaacccc ggggctgcag aaggatcgcg gccaccatca
      841 gtacgccgaa caccagagcg tggtgatgtc cgatgagcgg cgaaagcaga aatggcagga
      901 ggtagagcag caccgcgccg atgatcgcgc cgaggtggcg ccccgcgccc ccgacgatca
      961 ccatgaaaag cagcaggaag gcgttctcca ggccgaagat cggcgggttc acggtgttgt
     1021 ccacgaatgc ataggccatg cccgccaccc cggcgagcac gctcgtgagc gtgaaggcgg
     1081 tgactttggt gcgcgccaca tcgatgccca tggccgcggc ggcttcgggg ttgtcgcgca
     1141 ctgcgcggca ggctcggccg aaccacgagc ggtcgagccg cagcagtacc gcgtaggtgg
     1201 caaggacgag cacgacgatc acgacggtgt accaggggcc agtgatacgc acgccaaaca
     1261 ccggcggcag acggtagtcg acgaggcccc cggtgccgcc ggttacggct ccgccttcgt
     1321 tcagcaccac gatgaaaagc tgcgcaagcg cgagcgtagc gagcgcgagg tagaagccct
     1381 ctagccgggt agacggaatg gcgaccgcga gcgcgatcgc ggcgcaaacc aggacgcctg
     1441 cgaggatcgc gagcggcaga ggcatgccga gctggaaaag aaaaatcacc acggcgtagg
     1501 cgccgatgcc gtagaacacg aagtgcgaga acgcgacctg cccggaaatg ccgaacagga
     1561 agttgtagct gagcgccagc gcgatccaga ggagcagctt gcggtacacg tcgttgtagt
     1621 agctgctcag gaaaaaggcc gccgccgaca tgaccagcgc cgcggcgacc cacccggcga
     1681 gcgcggtggc gtttttcgtg ttcatccccg cgcgcgcagc aggccggtcg gccgcacaag
     1741 gaggaagagc atcagtaaga cgagcggcac gccctgcgca tagccggcgg gaaggggcgc
     1801 gtagcgtacg gtgagctgct cgacgaacgc gacgagcagt ccgccgagca gcgcgccctc
     1861 gacgcgcccg acgccgccga tcacgagcgc ggtgaagccc tggatggcga gcggaacgcc
     1921 catcagatag tgcgccgaga cgttgggggc gacgagcagc ccggcgagtc cggccgccgc
     1981 cgcgccgccg gcaaaggaag ctgcggtgac gcgcgccaga tcgatgccta ccagcgcggc
     2041 ggctttgcgg ttgagcgcca ccgcctcgaa gcttttgccc cagacggttc tttcgaagaa
     2101 gagccacacc gccgcgaaca cgaggagcgt cgtgccgatc atgaagtact tgaggtattc
     2161 ccagctgacg ggctcgggca cctggtgcgg gtcggggccg aagccgagcg agatcgactc
     2221 gcgaaatacg accagcatgc cgaggagcgc gagaatcggg gcgagaagcg gcgcgccgcg
     2281 ctccaccagc gggcgcaccg taatgcgctc ggtcgcccag ccgacgagga gcatgaagcc
     2341 gaggatcgcc gccatgccga gcgggtacgg ccactcgaat ctcgaaagca gcagaaaccc
     2401 gccaaaggct ccgaccagcg cccactcgcc caccgcaaaa ttgatgatcc cggtcggccg
     2461 gatcatcagc agataggcga gcgcgactag cgcatagatg cagcccgaag cagtcgcgct
     2521 tatcgagagt tcgagaagat cctgcaacga ggcgttaata cctacttctg gccgacatgg
     2581 accacgcggc cttgcttgtc tccctcgtac acgcgcacca ccatgccatt cggatcgaag
     2641 ccgatgcgct tgccttcggg gaacacatac ttcattccct tcgcgcccat gacgcccgaa
     2701 aaggtgagcg actgcatcgc ggcgcggatc ttctccttgt cggtcgaccc ggctcgcctg
     2761 atcgcctcgg cgaccatgcg gatgccgtca tagccgtagc cgtggatgtt gtagggctgc
     2821 ttgccggtcg ccttctggta atccgcctcg aacttttgca cctccggctt atccgggtcg
     2881 taggcgtcta cgaaggtgat gccgttcagc aaccccttgg cgaccgacat gtactgcggc
     2941 accgagaggt tgtaggacga atggatcggg aacttgagac ccatctggcg gtaggcgcgc
     3001 agaatcagca cattttcagg cgtcagcacg cccgccatga acaacgcgtc cggattggcg
     3061 gcgcggatct tctgcatctg tggctcggcg gaggtagcgc ctcgcggcac gacctcctcg
     3121 gtcaccacct ggatgcccga cttctccagc cccgcacgaa agaacttcgc gagcagctgt
     3181 ccgaggtcgg agttgtcgct gatcagagcc acgcgcttca gctgctttgc tctcgcatga
     3241 gacagcaacg tggcaatctg gaccgagccg agcgggccag tctgccagaa atacgggttc
     3301 ggcagctggg tggtcagcgt gtcgcccgag ttcatcggcg tcatctgcgg cgtcttcgct
     3361 tcgagcgtga cgttctgctg ctgctgcgtc gccggcgtga gagtaatgct gaggatgaag
     3421 accgcgcccg cgtcgacgag cttgcgcgcc atcgccgccg agatatctgg gcgaccctgg
     3481 tcgtcttcga tcaggagctc gatcttgcgg ccaagcaggc cgcctgcttt gttgatctcg
     3541 tcggcgccga agaggatgcc ctcgagcgtc ggctccgagt acggcttcgc aggcccgacc
     3601 ttgtcggtaa tcaccccgat cctgatcgtc tcctgtgaga aaaccgaggc cgcgaacagc
     3661 gcgagcgcta ccgcgaaaag cttcctcatg agcccctcct cttcattcga gcagacctcg
     3721 cctcgccatc catcctacac aaagttccac cgcctgctcg agcagttccg gctgcccctg
     3781 gtagtagtgg gtcgcaccct ggatgacgtg gaaggccttg tcgcggctcc ccgccgcatc
     3841 atggatgatg cgcgcgtgcg gctgcggcac cgcgtcatcg gcggagttct cgatcgcgag
     3901 cagcggcacg ctgatcgagc gcgcgcactt tggcccatcg gcgttcgagt cgtcgatcga
     3961 ccactgggaa agccacgcgc gcagcgtcga gaagcggccg atccccacgg gtccggtatt
     4021 cgcggtctcc ggcttgccga ggtagcacca gcgcggcttg cggtcgttcg ggtcgagcgc
     4081 cgggtccatg aagcgcacgt cgcacatcgt ccggtaggtg acgaagccgc gctccatttc
     4141 atcgcccttg agctttcgta aagcctctct tacccacccc gtcctccggc gcatcctgcg
     4201 tagctgtgcg gagcgaaacg tctccacgaa atccttcgag taaggcggct ggttgggaca
     4261 cctggggtcg tagatgtcga gctcaaggtt gcgctcgtcg gggtttgcct catcggtgac
     4321 cgacggatcg atccattccg acagcatgcg tgcccgcgaa aggtgcgcgg cctggaagat
     4381 catggcgtcg gccggtatca acgctttcag ctctaccggg tctcccgcag gcgtctcggt
     4441 tatcgaaggc ttttctgcct gcgattggta gaacagcgca agcgacccac cgcccgacca
     4501 gccacagagc accaccctgt cgtagcccca cacctccttc gcccggcgga tgtaggcgcc
     4561 gaggtccagc agcacctttt ccatgatgag cggggtgtca ttcttcgcga agcgcgagcc
     4621 cgcgcacagc acatgcacgc cggcgcgcgc aagcgcctgc ggcatcggca ggagctgaag
     4681 cgtggacgaa gggtgcatga agacggcgag cgtcttcgac ggcctgcctt tcggcaggaa
     4741 gcgtacgccc tcgcagttga ccgcgccctg cgtgccggcg aagccgtaga cctcggtgaa
     4801 cttggcggct tccttgtact gcacatgcac ccattcgggt gcaagcgaga atttcacgcg
     4861 ccgtttctac ttcttgtgaa cccagtcggc gtggtggggc gctttcttgg ttttcgacca
     4921 ctcctggagc atctcgtcgc ttacggcggc gagccgcttc ttcatgtccg gcgtggtggt
     4981 gtccacggcg agctcgagcc ggtggccgtt ggggtcgaag aagtagatcg acttgcagat
     5041 ggtgtgatcc gtgggcccca ccacctcgat ccccagcttc tccagcttgg ctttgtaggc
     5101 catgagcgcc ttttcgtcgg gcacgcgcag cgcgaggtgc tggacccaga gcggcgtgtt
     5161 ctggtcacgc cccatgggcg gggactccgg cacctcgaaa aacgcgacat agctgccgtc
     5221 ggccatgcga aagaagatgt gcatgtacgg cgagtactcg cccgtgctcg gcacgtggtc
     5281 ttcggccatc gccatcgcgt actcgaggcc cagcacccgg gtgtagaaat cggcggtctc
     5341 tttcgcgtcc ttgcagcgat acgcgacatg gtgcagagat ttgatgtcca tataatcgat
     5401 tatttcctac ggaggagaca tggacaagcg caatcaccag gaacgccatg acgcggggct
     5461 caagacccgc cgcgaggtgc tcggtcccgc ctacgtcgac aaggcgatgg cgggggtgga
     5521 cgacttcagc cgggaattcg tggagctgct caacacctac tgctggaacg acatctggaa
     5581 ccgtcccggc ctgccgcgca agacgcgctc gatcctcaac ctcggcatgc tgtgtgcgct
     5641 cggcaaggag cacgagctca agctccacat ccgcggcgcg ctcaacaacg gactgagcaa
     5701 ggaggagatc aaggaggtgt tcctccaggt cgcgatctac tgcggcgtcc cggctgcggt
     5761 agtcgcgttc cgctgcgcga aggaggtctt ccagagttga agatcatcgg ctttatcggc
     5821 ctgggcgtaa t
//
LOCUS       DASTPD010000353         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_192224, whole genome shotgun sequence.
ACCESSION   DASTPD010000353 DASTPD010000000
VERSION     DASTPD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192224"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFR57_11945"
     CDS             <1..759
                     /locus_tag="VFR57_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0260113.1"
                     /translation="AVVNQKGGVGKTTTTVNLAAALAQAGRRVLLVDVDPQGNATMGS
                     GIDKRTLARTVYHALLGLGELSGIRVRAERGGYDLVPANRELAGAEVELVELPSRETR
                     LKAALERVAADYDYILIDCPPSLSLLTVNALAAAHRVLIPMQCEYYALEGLSDLVGTI
                     KRVRANLNPALDIAGLLRTMYDPRNTLSQQVSRELEAHFGDKVFRTLVPRNVRLAEAP
                     SYGVPAVLWDPSSKGAQAYMALAGEMLEREMAYS"
     gene            756..1592
                     /locus_tag="VFR57_11950"
     CDS             756..1592
                     /locus_tag="VFR57_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0260114.1"
                     /translation="MKAKGLGRGLDALLGSATEAGPRDALLTLPVARIRPGRYQPRTK
                     MDQQALAELAASIRSQGLIQPLLVRPVDRDRYELIAGERRWRAAQMAGLDEVPALVRE
                     VADEAALAMSLIENIQRENLNPMEEAAGIQRLVDEFRMTHEQAADAVGRSRSATTNLL
                     RLLKLARPVQEMLMEGALEMGHARALLALDGARQIEAGKRVAAKGLSVRETEALVNAL
                     LQGPAVQRKRVDRDLARLEEEISERLGTTVEIRAGKKGSGKLVVHYSSLDHLDGLVKK
                     LR"
     gene            1589..2017
                     /locus_tag="VFR57_11955"
     CDS             1589..2017
                     /locus_tag="VFR57_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU0260115.1"
                     /translation="MSAEFGYTFAALLAAKKSNAMLRQLSKPIRTVIAWQAAATLAMT
                     LAGAAFIGIHGAVSAAAGGLVSIIAGLAAASIAARGSGNSAGGILAGALRAEAVKIGL
                     VVLLLWLVFANYADVIVVVVIGSFVVTMLIFAMAFFVREY"
     gene            2027..2851
                     /gene="atpB"
                     /locus_tag="VFR57_11960"
     CDS             2027..2851
                     /gene="atpB"
                     /locus_tag="VFR57_11960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0260116.1"
                     /translation="MAAGKALTPTEYIQHHLTFFTKPVGDGGFWTLHVDSLVTALLAG
                     IVGIGFIWWVVRGATSGVPNKRQAFVELAFEFIDDQVKGVFHHGDRNRFVAPAALTVF
                     IWVLLMNAMDFLPVDIMAKILGAVGLHEWRFVPTADVNTTFALALSVWLLMIFFAIAV
                     KGLGGWIHELTCTPFGVKPWAAAPFLVLFNILFNLIEYISKPLSHSLRLFGNMYAGEI
                     IFLLLWLWAATGLVGAIFAVPLALGWAIFHILIVVLQAYIFMMLTVVYIALAHEHH"
     gene            2894..3169
                     /gene="atpE"
                     /locus_tag="VFR57_11965"
     CDS             2894..3169
                     /gene="atpE"
                     /locus_tag="VFR57_11965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU0260117.1"
                     /translation="MEKLQLLAMVQAYTGIGIGLMIGVGAAGACIGVGIMCSRFLEAA
                     ARQPELTNSLQAKVFLLLGLIDASFIIGVGLAMMFAFANPLILPEFR"
     gene            3200..3670
                     /locus_tag="VFR57_11970"
     CDS             3200..3670
                     /locus_tag="VFR57_11970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU0260118.1"
                     /translation="MNINITLIAQAVTFALFIWFTVKVVWPYMQRAIEARQKTIADGL
                     AAADEGKRSLELASQRSEEEIRNARDRAAELLSHAEKRGAQLIEEAKNAAKEEGNREK
                     AAAKAEIEQEMTRAREQLRDHVASLAVAGAEKILRREVDAKAHGELLESLKRQL"
     gene            3670..>4146
                     /locus_tag="VFR57_11975"
     CDS             3670..>4146
                     /locus_tag="VFR57_11975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU0260119.1"
                     /translation="MAEHSTVARPYAVAAFNLAVETGTLAKWSEMLAALALVAAADRV
                     RAAASDPNLTEAQVAGLFISILSGRLTGDAENFVRVLAENGRLALLPEIRTQFEALKN
                     EHEGVLVAEIHAAFQLSDSQLADLTQRLEKKTGRKVRIKVQLDKGLIAGVRIVLGDK"
BASE COUNT          720 a         1321 c         1367 g          738 t
ORIGIN      
        1 gccgtagtga accagaaggg cggcgtcggc aagacgacga cgaccgtgaa cctcgccgcg
       61 gcgcttgcgc aggcgggacg gcgcgtgctc ctcgtcgacg tcgacccgca aggcaacgcc
      121 accatgggca gcggaatcga caagcgaacg ctggcgcgca ctgtctatca cgcgctcctc
      181 ggcttggggg agctctcggg catccgcgtg cgcgccgagc gcggcggcta cgacctcgtt
      241 ccggcgaatc gcgagcttgc cggcgcggaa gtagagttgg tggagctgcc ttcgcgcgag
      301 acgcgactca aggcagcgct tgaacgggtc gccgccgact atgactacat cctcatcgac
      361 tgcccgcctt cgctcagcct gcttacggtg aacgcgctcg ccgcggctca ccgcgtgctg
      421 atcccgatgc agtgcgagta ctacgcgctc gaaggtcttt ccgatctcgt ggggacgatc
      481 aagcgcgtgc gtgccaacct caatccggcg ctcgacatcg cggggctgct acgaaccatg
      541 tacgacccgc gtaatacgct gtcccagcaa gtatcgaggg agctggaggc gcacttcggc
      601 gacaaggtgt tccgcacgct cgtaccgcgc aatgtgcgcc ttgccgaagc cccgagctat
      661 ggcgtgccgg cggttctgtg ggatccgagc tcgaagggcg cccaagcgta tatggcgctc
      721 gcgggcgaga tgcttgaacg ggagatggct tactcatgaa ggcaaaaggt ctcggacgcg
      781 gtctggacgc gcttctcggc agcgctacgg aagccggacc gcgcgatgcg ctgctcacgc
      841 tgccggtcgc acgcattcgg cctgggcgct accagccgcg gaccaagatg gaccaacagg
      901 cgctcgccga gctcgcagcg tcgatccgtt cgcaggggct gatacagccg ctgctcgtgc
      961 ggccggtaga tcgcgaccgc tatgagctga ttgccggcga gcggcgctgg cgcgcggcac
     1021 aaatggctgg actcgatgaa gtacccgcgc tcgtgaggga agtggccgac gaagccgcgc
     1081 tcgcgatgtc gctcattgag aacatccagc gcgagaacct taatcccatg gaggaagccg
     1141 ccggcatcca gcgcctggtc gacgagttca ggatgaccca tgaacaggcc gccgacgcgg
     1201 tcggacgttc gcgcagcgcc accactaatc tgctgcggct cctgaagctc gcgcggccgg
     1261 tgcaggagat gctgatggaa ggcgctcttg agatggggca cgcgcgcgcg cttctcgcgc
     1321 ttgacggcgc gcgccagatc gaggccggca agcgtgtcgc ggcaaagggg ctctcggtgc
     1381 gcgagaccga ggcgttagtg aatgcgctgc tgcaggggcc ggcggtccaa cgcaagcgcg
     1441 tcgaccgcga cctcgcgcgg ctagaggagg agatctccga acggctcggc acgacagtgg
     1501 aaatccgcgc aggcaagaag ggcagtggca agctcgtggt gcactactcg agccttgatc
     1561 acctcgacgg gctcgtgaag aagctccggt gagcgcggag ttcggatata ctttcgcggc
     1621 tttgctcgct gcgaagaaat cgaacgccat gcttcgccag ctgagcaagc cgatccgcac
     1681 cgtgatcgcg tggcaggcag cagccacgct ggcgatgacg ctcgctggag cggcgttcat
     1741 cggcatccac ggcgcggttt ccgcggccgc cgggggtctc gtcagcatca tcgccggctt
     1801 ggcggccgca tcgatcgctg cgaggggtag cgggaattcg gcagggggga tcttggccgg
     1861 ggcgctgcgc gccgaagccg tgaaaatcgg cctggtcgtt cttctgctgt ggctggtgtt
     1921 tgcgaactac gccgacgtga ttgtggtcgt cgtcattggc tcgttcgtgg tcaccatgct
     1981 catttttgcg atggcgttct tcgttcgcga gtactgacgc attcagatgg ccgcaggcaa
     2041 ggcgcttacc ccgacggaat acatccagca ccacctcacg ttcttcacca agccggtggg
     2101 ggacggcggt ttctggacac tgcatgtgga ctcgctggtg acggcgctcc tcgccggtat
     2161 cgtgggtatc ggcttcatct ggtgggtggt gcgcggagcg acctcgggcg tgccgaataa
     2221 gcgccaggcg ttcgtcgagc tcgccttcga gttcatcgac gatcaggtca agggcgtttt
     2281 ccatcacggc gaccgcaacc gcttcgtcgc tcctgccgcg ctcacggtct tcatctgggt
     2341 tctgctgatg aatgccatgg attttctgcc ggtggacatc atggcgaaga ttctgggcgc
     2401 cgtcggcctg cacgaatggc ggttcgtgcc cacggccgac gtgaatacca cgtttgcgct
     2461 cgcgctgtcg gtgtggctac tcatgatctt cttcgccatt gcggtcaagg gcctgggcgg
     2521 ctggatccac gagctcacct gcacaccgtt cggggtaaaa ccgtgggcgg ccgcgccttt
     2581 tctcgtgctg ttcaatatcc tcttcaacct catcgaatac atttcaaagc cgctgtcgca
     2641 ctcgctgcgg cttttcggca acatgtacgc gggcgagatc atcttcctgc ttctgtggct
     2701 gtgggcggcg accggcctgg tcggcgccat cttcgcggtg ccgctggcgc tcggctgggc
     2761 gatcttccac atcctcattg tcgtgctgca ggcctacatc ttcatgatgc tcaccgtcgt
     2821 ctacatcgcg ctcgcgcacg agcatcactg accgtcatcc aacattcaat cgctcaggaa
     2881 aggacaaata ccgatggaaa aactgcagct tctggcgatg gtccaggcct acaccggcat
     2941 tggcatcggc ctcatgatcg gcgtcggcgc tgccggcgcc tgtatcggcg tgggcatcat
     3001 gtgcagccgc ttcctcgagg cggcggcgcg ccagccggaa ctcaccaact ctctgcaggc
     3061 gaaggtgttc ctgctgctcg gcctgatcga cgcctcgttc atcatcggcg tgggcctcgc
     3121 gatgatgttc gctttcgcca acccgctgat cctccccgag ttccgctaga gagcactccg
     3181 ttctgcagaa agacggaaca tgaacatcaa tatcactctg atcgcgcagg cggtcacctt
     3241 cgcactgttc atctggttta cggtgaaggt ggtgtggccg tacatgcagc gcgcgatcga
     3301 ggcgcggcag aagacgatcg ccgacgggct cgcggcggcc gacgagggaa agcgctcgct
     3361 cgagcttgcc agccagcgct ccgaagagga gattcgcaac gcccgcgatc gcgccgccga
     3421 gctcctgtcg cacgcggaga agcgcggggc gcagctgatc gaggaagcga agaacgcggc
     3481 caaggaggaa ggcaaccgcg aaaaggcggc ggccaaggcc gagatcgaac aggagatgac
     3541 gcgcgcgcgc gagcagctgc gcgaccacgt ggcttcgctc gccgtcgccg gcgccgaaaa
     3601 gatcctgcga cgcgaagtcg acgccaaagc gcacggcgag ctgctcgagt cgctgaagcg
     3661 gcagctttaa tggccgaaca cagcacggtc gcgcgtcctt acgccgtagc ggcgttcaac
     3721 ctcgccgtgg aaaccggcac gctcgccaag tggtccgaga tgctcgccgc gctcgcgctt
     3781 gtcgccgccg ccgaccgcgt gcgcgccgcg gcttccgacc ccaacctgac cgaggcccag
     3841 gttgcgggcc tgttcatctc gatcctctcg ggccggctca ccggggacgc cgagaacttc
     3901 gtgcgcgtgc tcgccgagaa cggcaggctc gccctgctgc ccgaaatccg cacccagttc
     3961 gaggcgttga agaacgagca cgagggcgtg ctggtggccg aaatccatgc agcgttccag
     4021 ctctccgatt cccagctcgc cgacctgacg cagcgcctcg agaagaagac cgggcgcaag
     4081 gtcaggataa aggttcagct tgacaagggc ctcatcgccg gagtgcggat cgtcctcggc
     4141 gacaag
//
LOCUS       DASTPD010000354         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1623836, whole genome shotgun sequence.
ACCESSION   DASTPD010000354 DASTPD010000000
VERSION     DASTPD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623836"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..541)
                     /locus_tag="VFR57_11980"
     CDS             complement(149..541)
                     /locus_tag="VFR57_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0260120.1"
                     /translation="MLNLRGIDHVVLRVADIEMMRRFYCDVLGATHVAYRPQFGMSHL
                     RVGQAMIDLVEAKGPVGQGRNMDHLCLRVEPFDQQAVVAHLERHGVKVGDIRNRFGAE
                     GNGISIYITDPEGNTVELKGPSDGRAEP"
     gene            707..1072
                     /locus_tag="VFR57_11985"
     CDS             707..1072
                     /locus_tag="VFR57_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260121.1"
                     /translation="MKNADETVRSSLTQQKDTAASSLGSFAGALRKAARESDGGEGTA
                     AHMAEWAADGLERISSTLRTKDLNGMVREVESFARSQPVAFFVCAAAAGFLATRFLKA
                     GSRDSQPQRQTDFTSMTRV"
     gene            1086..1520
                     /locus_tag="VFR57_11990"
     CDS             1086..1520
                     /locus_tag="VFR57_11990"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0260122.1"
                     /translation="MASIPQQDFPEREYAQGRSLMGLFADLWRETQTLVHQEAQLAKA
                     ELSQKVSQVATGAGEIAAGGAVLFAGFIVLLFAAVGALELILDTEHSVWLSPLIVGLV
                     VMIIGYVLLSRGRKQITAEKLTPERTMQSLQRDARLAKEHAT"
     gene            1517..>2076
                     /locus_tag="VFR57_11995"
     CDS             1517..>2076
                     /locus_tag="VFR57_11995"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEU0260123.1"
                     /translation="MRNNGELKHRSNGEKRPEEILAEIDRTRDEMDRTLSAIEHRLTP
                     GQLVDQGLDYLRHSGAAEYVQNLGGTAKQNPLPIALAAIGIGWLMALGRQPAQQSYGA
                     TSESGLREGIGSMRNKASSAMQSTSDSLSSAKDRVTGSISSMRDRAGQMTDSAKYQWE
                     RARGGVDYLVHEQPLALGAIGLAVGAV"
BASE COUNT          400 a          689 c          660 g          327 t
ORIGIN      
        1 tgctgctcgt cgagcagaac gccgagatgg cgctgcggct cgccgaccgc gtctacgtaa
       61 tcgatcacgg cactgttgtc ttcgaaggca cgccagccgc gctgcgcgcc gaccggcagg
      121 tgacgaccac ttacctcggc gttggcggct agggctcggc ccttccgtcg gagggcccct
      181 tcaattccac ggtattgcct tccgggtcgg tgatgtagat cgagattccg ttcccttcag
      241 cgccgaagcg gttgcggata tcccccacct tcacaccgtg ccgctcgaga tgcgcgacta
      301 cggcttgctg gtcgaacggc tctacgcgca ggcagaggtg atccatgttg cggccctgcc
      361 cgacagggcc tttcgcctcg acgagatcga tcatcgcctg gccgacgcgc aaatgcgaca
      421 tgccgaactg cggccgataa gccacatgcg tggcgccgag cacgtcgcaa tagaagcgcc
      481 gcatcatttc gatgtccgcg acgcgcaaca ctacgtggtc aattccgcga aggttcagca
      541 tttcgcctac gaatcttccc tcagtccgac caccagcgcg cgcatggcac ttgcatctcc
      601 ccgcgcattc cctcggaaag gagcggccga tatggctacg acaccctcac cacaggaccc
      661 cctgcagcgt gcaacgcaat ctgcgaagga gcaggcgcgc gacgtcatga aaaacgctga
      721 cgagactgtg cgttcgtccc tgacacaaca gaaggatacc gcggcatcga gcctcggaag
      781 cttcgccggg gcactgcgca aagcggcgcg cgaatcggac ggcggcgaag gcacggcggc
      841 gcacatggcc gaatgggcag ccgatgggct ggagcgcatc tccagcacgc tgcgcacgaa
      901 agacctgaac ggcatggtgc gtgaagtcga atcgttcgcg cgcagccagc cggtcgcgtt
      961 cttcgtctgc gccgcggccg cgggttttct cgcgacgcgc ttcctcaagg ccgggagtcg
     1021 ggacagccag ccgcaacgcc agaccgactt cacctccatg acccgcgttt agcagaggac
     1081 attcaatggc atcgataccg caacaagact ttcccgaacg cgagtacgcg caaggacgtt
     1141 cgctgatggg gcttttcgcc gacctgtggc gcgagacgca gaccctagtg caccaggagg
     1201 cccagctcgc gaaggcggag ctctcccaga aagtgtcgca ggtcgccacg ggggccggcg
     1261 agatcgccgc gggcggcgcc gtgctcttcg ccggattcat cgtcctgctc ttcgcggcgg
     1321 tgggcgcgct cgagctcatc ctcgacaccg agcattccgt ctggctctcg ccgctcatcg
     1381 tcggactggt ggtcatgatc attggctacg tccttctttc ccgcggtcgc aagcagatta
     1441 cggcggagaa gctcacaccc gagcgcacga tgcaatcgct gcagcgggac gcaaggcttg
     1501 caaaggagca cgcgacatga ggaacaacgg cgaactgaag caccgcagca acggcgagaa
     1561 gcgccccgag gagatcctgg ccgagatcga ccgcacgcgc gacgagatgg accgcacgct
     1621 ctcggcgatc gagcaccgcc tcacgccggg tcagctcgtg gatcaaggac tggattacct
     1681 gcgccatagc ggcgcggcgg aatacgtgca gaacctcggc ggcaccgcca agcagaaccc
     1741 gctgccgatc gcactcgctg cgatcgggat cggctggctc atggcgctcg gtcgccagcc
     1801 cgcgcagcag agctacggcg cgacatcgga atccgggttg cgcgagggca tcggatcgat
     1861 gcgcaacaag gcgagcagcg cgatgcaaag cacatccgac agcctgagct cggcgaagga
     1921 tcgggtcacg ggcagcatat cgtcgatgcg cgaccgcgct ggccagatga cggactcggc
     1981 gaagtaccaa tgggagcgcg cgcgtggcgg cgtcgactat ctcgtgcacg aacaaccgtt
     2041 ggccctcggc gccatcggtc tcgcagtcgg agcagt
//
LOCUS       DASTPD010000355         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1624260, whole genome shotgun sequence.
ACCESSION   DASTPD010000355 DASTPD010000000
VERSION     DASTPD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624260"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFR57_12000"
     CDS             complement(<1..377)
                     /locus_tag="VFR57_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU0260124.1"
                     /translation="MNAGLIFALVCALLAIVYGLWQSARILRLPAGNERMREISAAVR
                     EGAVAYLWRQYKTIAAVGAVLFVLIGLFIDWASAWGFFIGAVLSGACGIIGMNISVRA
                     NVRTAEAARVGLNEALQVAFRGGA"
     gene            complement(412..1047)
                     /gene="adk"
                     /locus_tag="VFR57_12005"
     CDS             complement(412..1047)
                     /gene="adk"
                     /locus_tag="VFR57_12005"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU0260125.1"
                     /translation="MRLILLGPPGAGKGTQAKFICENFGIPQISTGDMLRSAIKAGTA
                     LGKQAQKVMDTGQLVADDIIMGLIRERLRQNDCKRGYLFDGFPRTIPQAEAITREGVD
                     IDYVLEIDVPDEEIVMRMSGRRVHPGSGRVYHVKFNPPKAQGRDDVTGEPLVQREDDR
                     EDTVRKRLEVYRAQTRPLVDYYQRHAAGKYRRISGRGSVEDVRDRTLAALS"
     gene            complement(1044..1769)
                     /gene="kdsB"
                     /locus_tag="VFR57_12010"
     CDS             complement(1044..1769)
                     /gene="kdsB"
                     /locus_tag="VFR57_12010"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU0260126.1"
                     /translation="MKFSVIIPARYASTRFPGKPLADLADKPMVVRVCEQAAKSGAAA
                     VHVATDDRRIAQAVERAGFAAVMTSAAHASGTDRVAEAAKKLKLKRDQVVVNVQGDEP
                     LISPRLIRAVGTLLEKRRDASVATACHAIHNAAALVNPNVVKVVLDAAGYALYFSRSQ
                     IPYPREPGAICYRHAGIYGYRVGFLKRYTRLEPSPLEQSEALEQLRVLWHGYRIAVAV
                     SKAEIPPGVDTPQDLDAVRRIVS"
     gene            complement(1779..1970)
                     /locus_tag="VFR57_12015"
     CDS             complement(1779..1970)
                     /locus_tag="VFR57_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU0260127.1"
                     /translation="MSAVDPRLLEILVCPLCKGKLVYRKPAGELVCKADRLAYPVKDG
                     IPVMLEEEARKLPPEEEVN"
     gene            complement(1942..2916)
                     /gene="lpxK"
                     /locus_tag="VFR57_12020"
     CDS             complement(1942..2916)
                     /gene="lpxK"
                     /locus_tag="VFR57_12020"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU0260128.1"
                     /translation="MRFWYRRGAIAWLLWPASLAFGIGVALRLLLYRFGVCKSAHPGI
                     PVIVVGNLTVGGSGKTPLVIWIAEFLKSQGRSPAIIARGYGAKLREPRAATIASEAAE
                     VGDEPVVLARRSGCPVWVGPNRVDVAVALRAQHPEVDVLVLDDGLQHYRMRRDIEIAV
                     VDARGFGNGFLLPAGPLREPRLRLRTVDAVVAHGARVAGYAMKLEGEELHRMSDARER
                     RPLKSFAGQKVHAAAGIGDPNRFFLHLASAGLKVIPHPFPDHHPFSVRELEFDDGLPV
                     ILTEKDAVKLRSRARPEWWVLPVSAKLDTRFGAWLLGKLDECRRSKAA"
     gene            complement(2917..3333)
                     /locus_tag="VFR57_12025"
     CDS             complement(2917..3333)
                     /locus_tag="VFR57_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0260129.1"
                     /translation="MNFQRGKEKEPLEINLVPMIDVMLVILIFLMITTTYSKYTELQI
                     NLPSARADRQVQRPNEVTVLVNAQGQYVVNRSPVPFRSIEQLADELRRAAAPLQEPVM
                     VISADANATHQAVIRVMEAARLAGLAQITFTTQSPK"
     gene            complement(3330..3941)
                     /locus_tag="VFR57_12030"
     CDS             complement(3330..3941)
                     /locus_tag="VFR57_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0260130.1"
                     /translation="MFAIVQAAGWPIWFLLFASVIAVALIIERSISLRASRIIPPTLL
                     DQVIAVYQRQGLSPEVINRLSRDSPLGVVLAAGLRNLRSSRYVMKEAIEEAGRGVAHE
                     LGRVLTTLGTIATAAPLLGLFGTVIGMIEIFGSQSPTGGTNPQQLAHGISIALYNTAF
                     GIAIAIPALIFYRHFRAKVDGFVVEMEQLAAKLIDIVHGERSA"
BASE COUNT          730 a         1392 c         1338 g          713 t
ORIGIN      
        1 gcgccgccgc ggaatgccac ctgcagcgcc tcgttcaggc cgacgcgcgc cgcttcggcg
       61 gtgcgcacgt tcgcgcgcac cgagatgttc atgccgatga tgccgcaggc gccggagagc
      121 accgcgccga tgaagaagcc ccacgccgaa gcccagtcga tgaacagccc gatcaggacg
      181 aaaagcactg cgccgacggc cgcaattgtc ttgtactggc gccagagata cgcgaccgcc
      241 ccctcgcgca cggcggccga aatctcgcgc atgcgctcgt tgcccgcggg cagacgcaga
      301 atgcgcgcgc tctgccagag gccgtagacg atcgcgagta acgcgcagac gagcgcgaag
      361 atgaggcctg cgttcatagg atttatcccc ctgaaaaggg tcggaatttt atcagctcaa
      421 cgcagccagg gtgcggtccc tgacgtcctc gacgctgccg cggcccgaaa tccttcggta
      481 cttgcccgcg gcatgccgct ggtagtagtc cacgagcggc cgcgtctgcg cgcgatacac
      541 ctccaggcgc ttgcgcacgg tgtcctcgcg atcgtcctcg cgctgcacca ggggctcacc
      601 cgtgacatcg tcccggcctt gcgccttcgg cgggttgaac ttcacgtgat agacgcgccc
      661 ggagccggga tgcacgcgcc ggccgctcat gcgcatgacg atttcttcat cgggcacatc
      721 gatctcaaga acatagtcga tgtccacgcc ttcgcgggtt atcgcctcgg cctgcgggat
      781 ggtgcgcgga aacccatcga acaggtaacc gcgcttgcag tcgttctgcc ttagccgctc
      841 ccggataaga cccatgatga tgtcgtcggc cacgagctgg ccggtgtcca tcaccttttg
      901 cgcctgcttg ccgagcgcag tgcccgcctt gatcgcggag cgcagcatgt cgccggtgga
      961 gatctgcggg atgccgaaat tctcgcagat gaacttcgcc tgcgtgccct tacccgcgcc
     1021 cggcggaccg aggaggatga gcctcatgaa acgattctac gcaccgcgtc gaggtcctgc
     1081 ggggtgtcca cgcccggcgg aatctcggcc ttgctcaccg ctaccgcaat gcggtagcca
     1141 tgccacagca cacgaagctg ctcgagcgcc tcgctctgct cgagcggcga gggctcgagg
     1201 cgtgtgtacc tcttcaggaa gccgacccgg tagccgtaga tcccagcgtg ccggtagcag
     1261 atcgccccgg gctcgcgcgg ataaggaatc tgcgagcgcg aaaagtaaag cgcatagccg
     1321 gcggcgtcga gcaccacctt gaccacgttc gggttgacga gcgcggccgc attgtggatc
     1381 gcatggcagg cggtggcgac gctcgcatcg cgacgctttt ccagcagcgt tccgactgcc
     1441 cggataaggc gaggcgatat cagcggctcg tcgccctgaa cgttcaccac cacctggtcg
     1501 cgcttcaact tgagtttttt cgccgcctcg gcgacccggt cggtgccgga ggcgtgagcg
     1561 gcgcttgtca tgaccgccgc aaagccggct cgctcgaccg cctgcgcgat gcgccgatcg
     1621 tcggtcgcca cgtgcactgc cgccgctccg ctcttcgccg cctgctcgca cacgcgcacc
     1681 accatcggct tgtcggcgag gtcggcaagc ggctttccgg ggaagcgcgt cgaagcgtac
     1741 ctcgccggaa tgatgacgga aaacttcaag tcacgccttc aattcacttc ttcttctggc
     1801 ggcagcttcc tcgcttcctc ttcgagcatg acgggaatgc cgtccttcac cgggtaggcg
     1861 agccgatcgg ccttgcacac gagctcgccc gccggcttgc gatagaccag ctttcccttg
     1921 cagagggggc agacgaggat ctcaagcagc cttggatcga cggcactcat cgagttttcc
     1981 caggagccaa gcgccaaacc gggtatcgag ctttgcgctc accggcagca cccaccattc
     2041 gggccgcgcg cggctgcgca attttaccgc gtccttctcc gtgaggatca ccggcaggcc
     2101 gtcgtcgaac tcgagctcgc ggaccgagaa cggatgatgg tcggggaatg ggtgcgggat
     2161 caccttgagg cccgcactgg cgaggtggag gaaaaaccgg ttcggatcac cgatacccgc
     2221 cgcggcgtgc accttctgcc cggcgaagga cttgagcggc cggcgctccc gcgcatcgct
     2281 catgcgatgc agctcctcgc cttcgagctt cattgcgtat ccggcgacac gcgcgccgtg
     2341 cgcaaccacc gcgtccacgg tgcgcaaccg cagccgcggc tcgcgcaagg ggcctgcggg
     2401 cagcaggaag ccgttgccga agccgcgcgc gtccaccacc gcgatctcga tgtcgcgccg
     2461 catgcggtag tgctgcaagc cgtcgtcgag cacgaggacg tccacctcgg gatgctgggc
     2521 gcgcagcgcg accgccacgt ctacccggtt gggccccacc cacaccgggc agccgctgcg
     2581 gcgcgcaagc accaccggct cgtcgccgac ctcggccgcc tccgaggcga tcgtcgccgc
     2641 gcgcggctcg cgcagcttcg ccccgtagcc gcgcgcgatg atcgccggcg agcgtccctg
     2701 gctcttcagg aactccgcga tccagatcac gagcggcgtc ttgccgctgc cgccgacggt
     2761 caagttgccg accacgatca ccggaattcc cgggtgcgcg ctcttgcaaa cgccaaacct
     2821 gtaaagcaga agccgcagcg cgacgccgat gccgaacgcc aggctcgccg gccacagcag
     2881 ccacgcgatg gcgccgcggc ggtaccagaa cctcacttac ttcggcgact gcgtcgtgaa
     2941 ggtgatctgg gcgaggccgg caaggcgcgc ggcctccatc acgcggatca ccgcctggtg
     3001 cgtcgcgttc gcgtcggcgc tgatcaccat caccggctct tgcagcggcg cggcggcgcg
     3061 gcgcagctcg tcggcgagct gctcgatcga gcgaaacggc accgggctgc ggttgacgac
     3121 gtattggcct tgcgcgttca cgagtacagt cacctcgttc ggccgctgca cctggcggtc
     3181 ggcccgcgcc gaaggcaggt tgatctgcag ctcggtgtac ttggagtacg tggtggtgat
     3241 catgaggaaa atcagaatca ccagcattac gtcgatcatc ggcacgaggt tgatctcgag
     3301 cggctccttc tccttcccgc gctggaagtt cacgccgagc gctcgccgtg cacgatgtcg
     3361 atgagcttgg ccgcaagctg ctccatctcg accacgaagc cgtccacctt ggccctgaaa
     3421 tggcggtaga agatgagcgc ggggatcgcg atcgcgatgc cgaatgcggt gttgtagagc
     3481 gcaatcgaga tgccgtgtgc gagctgctgg gggttcgtgc cgccggtggg cgactgtgag
     3541 ccgaagatct caatcatgcc gatcaccgta ccgaacaggc caagaagcgg cgccgcggtg
     3601 gcgatggtcc ccagagtggt taggacgcgc ccgagctcgt gcgccacgcc gcggcccgcc
     3661 tcctcgatcg cctccttcat cacgtagcgc gaacttctta ggttgcgcag gcctgcggcg
     3721 agcacgacgc cgagcggcga atcgcgggaa agacgattga tcacctccgg cgaaagtccc
     3781 tgccgctggt agaccgcgat cacttggtca agcagcgtcg gcgggatgat gcggcttgca
     3841 cgcagcgaga tcgagcgctc gatgatcaga gcgactgcga tgacggaggc gaaaagcagg
     3901 aaccagattg gccagccggc ggcctggacg atcgcgaaca acgcttttcc ttatcgttat
     3961 tttggctgct tgcgcgccga ctctaaggca aagcgttgca gcggagcaag ttccgcgctg
     4021 cagcaaaagc gccgcatggc cctacggcga agatcatgtg aggctgcaat gtagtggata
     4081 ttgcgatatc cacaaaaaac tgtggataag tttgtggatt gctgctgaat ggattgtcac
     4141 atgcccgttg aaaagcgggt cgagtcgctg gcg
//
LOCUS       DASTPD010000356         5053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1220284, whole genome shotgun sequence.
ACCESSION   DASTPD010000356 DASTPD010000000
VERSION     DASTPD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220284"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFR57_12035"
     CDS             complement(<1..900)
                     /locus_tag="VFR57_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0260131.1"
                     /translation="MAKPPVYDASAIQALKGLEPVRRMPGMYTHTVHPLHIVQEAIDN
                     AVDEALAGFGKKITVSVRKDGSVEVADEGRGVPVDIHPVEKKPAVEVAFTMLHAGGKF
                     SRSGDGVYHISGGLHGVGVAVSNALSKRCEVVVFREGQKHTIAFEGGEVKTKLKSEKG
                     KERKTGTIVRLWPDPKYFDSANIPLAELEHLVRSKAYLLPGLTMVLEVEGKDSKTWKY
                     ERGMAQYFEFMLQGRELVAPLFTGERYFKESNIPEVEPGEGAAWAIGWVAEGDIFADS
                     HVNLIPTRSGGTHEAGFRSGIFEA"
     gene            complement(941..1201)
                     /locus_tag="VFR57_12040"
     CDS             complement(941..1201)
                     /locus_tag="VFR57_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEU0260132.1"
                     /translation="MARPRKPHPGIVLLRDFLEPRRISQNALARELGVPPRRINEIVL
                     GKRGISADTAIRLARRFGNSPRFWMQLQADYDLEEAKRALTP"
     gene            complement(1244..1924)
                     /locus_tag="VFR57_12045"
     CDS             complement(1244..1924)
                     /locus_tag="VFR57_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0260133.1"
                     /translation="MVEVERAGPLARIFLDRPEKANALNSAMLTELGAAVRSLAGEEL
                     RAAVLTARGKTFCGGADTAELKSLTPATAGAFVERIHDVCRAIRELPVPVLAQMHGAV
                     IGAGLEIAAACDLRVAAEGTKFAMPEVRLGIPSVVEAALLPRLMGSGRAAWLVLTGEA
                     IDARRALEWGLIEEIGDGAALLKHLLAGEPRALRMQKQLLHLWDEASLATSVAASIEH
                     FSRAYSRR"
     gene            1923..2627
                     /locus_tag="VFR57_12050"
     CDS             1923..2627
                     /locus_tag="VFR57_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEU0260134.1"
                     /translation="MLSSYSREALKKVSTATLTTVLFKRGLRNVFIQGIFLLNRAAPR
                     MVGEAFTLRYIPAREDVDQLGAFEGRGHPQREAIEACPPGHVLVMDARRDATAATGGD
                     ILMTRLMVRGVSGVVTDGGLRDSPTIEKLDWPAYCGARSAPLNLVRHHAVESQVPIGC
                     GGVAVYPGDVLVGDAEGVVVIPAKMAEEVAKEAQAQTEFEDWVEAKVREGRSIFGLYP
                     PNAETKAEFEAYKKRR"
     gene            2735..4093
                     /locus_tag="VFR57_12055"
     CDS             2735..4093
                     /locus_tag="VFR57_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260135.1"
                     /translation="MLKWLTGAALALLVALPVAAQETIVVYFQKGFYPAEDKAVDDLV
                     EKFTKKTGVKVELSRYAPQEMIPKTVAALDAGNPPDMVYGDVFDFQVAGKWAFEGRLE
                     DVSDIINPMKDKFLPNTIETTYMWNDKEKKRAYYAFPVKRQTMHIQYWKDMLTEAGFK
                     ESDIPKDWKGYFTFWCGKVQEGYRKKTGKRVYSVGSPMGVDSSDSFYSFLTYMDAYDV
                     KLVNDDGKLNLGKETRQGLINAVRDYTETINKGCTPPSAINWKDPDNNVNFHNKTIIM
                     THNATISIAAKWLDDMNNEKLKPEERAQAKKNYEELIATAGFPNKPDGKPMQYRAAVK
                     VGVVFNNAKNKKRGKEFAAFLMQEENLNPYVEGALGRWYPVTKSGAASKFWTEDPHRK
                     IVHNQFSAGTVTFEFTKNWKFTILNNENVWAKAMNRVVTDKWPVEKAVDEMIKRIQEV
                     AG"
     gene            4146..5015
                     /locus_tag="VFR57_12060"
     CDS             4146..5015
                     /locus_tag="VFR57_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0260136.1"
                     /translation="MSSIAIPHVPAAPPRRGLTTWQAWGLILIAPYAIVFLAFVLYPV
                     SYGLWLARHPESYARLFADPIFVRTAINTVVFLLVAVNLKFLLALWLSGFFVHERVWI
                     RWLSVVFLLPWAVPSIPTIFSIRFMFNPEWGVVNSVIFRLTAQDGPNWLNDPHIGLGL
                     AMLTHIWKSLPFWTLILMAGRLAIPREQYEAAAVDGASAWQKFKYVTWPSLRTLYLSS
                     TILSMIWSLGDFNSVYLLTGGGPADLTHVLATLGIRYIRLDQVDLAMATIVVAMPLVL
                     PLVYYMMKHLSRE"
BASE COUNT          916 a         1624 c         1612 g          901 t
ORIGIN      
        1 cgcctcgaag atgcccgagc gaaagccggc ttcgtgcgtg ccgcccgagc gcgtggggat
       61 gagattcacg tggctgtccg cgaagatgtc gccttcggcg acccagccga tcgcccacgc
      121 cgcgccctcg cccggctcga cttccggaat gttgctttcc ttgaagtagc gctcgccggt
      181 gaaaagcggc gcgaccagct cgcgtccttg cagcatgaac tcgaagtact gcgccatgcc
      241 gcgctcgtac ttccaggtct tggagtcttt gccctccacc tccaaaacca tggtgaggcc
      301 ggggagcagg tacgccttcg agcgcacgag gtgctcgagc tcggcgagcg ggatgttcgc
      361 cgagtcgaag tacttcggat cgggccagag gcgcacgatc gtcccggttt tccgctcctt
      421 gcccttttcg ctcttgagct tggttttgac ctcaccgcct tcgaaggcga tggtgtgttt
      481 ctgtccctct ctgaaaacca cgacctcgca gcgcttcgaa agcgcgttag agaccgcaac
      541 acccacgccg tgcaggccgc cggagatgtg atagacgccg tcgcccgagc gcgagaactt
      601 gccgccggcg tgcagcatgg tgaacgccac ttcgaccgcg ggcttcttct cgaccgggtg
      661 gatgtcgacc ggcacgccgc ggccttcgtc ggccacttcc accgagccgt ccttgcgcac
      721 gctcacggtg atcttcttgc cgaagccggc gagcgcctcg tcgaccgcgt tgtcgatcgc
      781 ctcctggacg atgtgcagcg gatgcacggt gtgcgtgtac atcccgggca tgcggcgcac
      841 gggctcgagg cccttcagcg cctggatcgc ggatgcgtcg tagacgggcg gctttgccat
      901 atctagtgct ttcctggcgg gtcggtgagg actctgatct tcatggggtg agggccctct
      961 tggcctcctc gaggtcgtag tcggcctgca attgcatcca gaagcggggc gagttgccga
     1021 atcgtcgcgc tagccgaatc gccgtgtcgg cgctgatgcc gcgcttgcca agcacgatct
     1081 cgttgatgcg tctcggcggc acgccgagct cgcgcgccag ggcgttctgg ctgatgcgtc
     1141 tgggctcgag aaagtcccgc agcagaacga tcccgggatg tggcttacgc gggcgcgcca
     1201 tataacgcgg gccgttatat aacggattgc gttatggcgc aaatcagcgc cgggaatagg
     1261 cccgcgagaa atgctcgatg ctcgccgcca ccgaggtggc gagcgacgct tcgtcccaca
     1321 agtgcaacag ctgcttctgc atgcgcagcg cgcgcggctc gcccgcgagc aggtgcttca
     1381 gcagcgcggc gccgtcgccg atttcctcga tcaagcccca ctcgagcgcg cggcgcgcgt
     1441 cgatcgcctc gccggtgaga accagccagg cggcgcggcc cgagcccatg agccgcggca
     1501 gaagggccgc ctcgaccacg gacgggatgc cgaggcgcac ttcgggcatg gcgaacttcg
     1561 tgccttcggc cgcgacgcgc aggtcgcaag ccgcggcaat ctcgaggccg gcgccgatga
     1621 ccgcgccgtg catctgcgcg agcaccggga ccggcagctc gcggatcgcc cggcagacgt
     1681 cgtggatcct ttcgacgaag gcgccggcgg tcgcgggggt gaggcttttc agctcggccg
     1741 tgtccgcgcc gccgcagaag gtcttgcccc tggcggtgag caccgcggcg cgcaactcct
     1801 cgcccgcgag cgagcgcacg gccgcgccca gctcggtcag cattgcggaa ttcagagcgt
     1861 ttgctttttc cggccggtcg aggaagatac gggcgagcgg tcctgcgcgt tccacttcca
     1921 ccatgctttc atcatatagc cgcgaggcgc tgaaaaaagt ttccaccgcg acgctcacca
     1981 ccgtgctttt caagcgcggc ttgcgcaacg tgttcattca gggaatcttc ttgctcaacc
     2041 gcgccgcgcc gcgcatggtg ggcgaagctt ttacgctccg atatatcccc gcgcgcgagg
     2101 atgtggatca actgggcgcg ttcgagggcc gcggccatcc gcagcgcgag gcgatcgaag
     2161 cctgcccgcc cggccacgtg ctcgtcatgg atgcgcgccg cgacgctacc gccgccaccg
     2221 gcggtgacat tctcatgacg cggcttatgg tgcgcggcgt gtccggcgtg gtcaccgacg
     2281 gcggcttgcg cgactcgccc accatcgaaa agctcgactg gccggcgtac tgcggcgcgc
     2341 gctcggcgcc gctgaatctc gtgcggcatc atgcggtcga gagccaggtg ccgatcggct
     2401 gcggcggcgt ggcggtctat ccgggcgacg tgctggtggg cgatgccgaa ggcgtagtcg
     2461 tcattccggc gaagatggcc gaggaagtgg cgaaggaagc gcaagcccag acggaattcg
     2521 aggactgggt ggaagccaag gtaagagaag gccgctcgat cttcggactc tatccgccga
     2581 acgccgagac taaggcggaa ttcgaggctt acaagaaacg ccggtagtcg cttctcctta
     2641 gttcaaaacc gaattcgcac cctgaccccg aattcggttt gtttgttggt tttgtcgtta
     2701 tcatacaagc gcaaaccaag aggaggattt tgcgatgctc aaatggctga caggggccgc
     2761 gcttgccctg ctcgtcgcgc ttcccgtcgc ggcgcaggaa acgatcgtgg tctacttcca
     2821 gaaagggttc taccccgccg aagacaaggc cgtggacgac ctggtggaga agttcaccaa
     2881 gaagaccggc gtgaaggtcg agctctcgcg ctacgcgccg caggagatga tcccgaagac
     2941 ggtggccgca ctcgacgcgg gcaacccgcc cgacatggtg tacggcgacg tgttcgactt
     3001 ccaggtcgcc ggcaaatggg ccttcgaggg ccggctcgag gacgtgtccg acatcatcaa
     3061 cccgatgaag gacaagttcc tgccgaacac catcgagacc acctacatgt ggaacgacaa
     3121 ggagaagaag cgcgcctact acgcgttccc ggtgaagcgc cagacgatgc atatccagta
     3181 ttggaaggac atgctgaccg aggcgggctt caaggagtcg gatatcccga aggactggaa
     3241 aggctacttc accttctggt gcggcaaggt gcaggagggc taccgcaaga aaaccggcaa
     3301 gcgcgtgtac tcggtcggca gcccgatggg cgtcgattcg tcggactcgt tctactcgtt
     3361 cctcacgtac atggacgcgt acgacgtgaa gctcgtgaac gacgacggca agctgaacct
     3421 cggcaaggag acgcgccagg gcctgatcaa cgcggtgcgc gactacaccg agaccatcaa
     3481 caagggctgc acgccgccct ccgccatcaa ctggaaggac ccggacaaca acgtcaactt
     3541 ccacaacaag acgatcatca tgacgcacaa cgcgacgatc tcgatcgccg cgaagtggct
     3601 cgatgacatg aataacgaga agctcaagcc cgaggagcgc gcgcaggcga agaagaacta
     3661 cgaggagctg atcgccaccg ccggcttccc gaacaagccc gacggcaagc cgatgcagta
     3721 ccgcgccgcc gtcaaagtgg gcgtggtgtt caacaacgcg aagaacaaga agcgcggcaa
     3781 ggaattcgcg gccttcctga tgcaggaaga gaacctgaac ccctacgtcg agggcgcgct
     3841 cggccgctgg taccccgtga ccaagtccgg cgccgcctcc aagttctgga ccgaagaccc
     3901 gcaccgcaag atcgtgcaca accagttctc ggccggcacg gtgaccttcg agttcaccaa
     3961 gaactggaag ttcacgatcc tcaacaacga gaacgtgtgg gcgaaagcca tgaaccgcgt
     4021 ggtcaccgac aagtggccgg tggagaaggc ggtcgacgag atgatcaagc gcatccagga
     4081 agtggccggg taacgatctt gtagagagga aacttcgggc ccgaagtttc gggcccgatt
     4141 tttttatgtc ctcgatcgcg attccgcacg ttccggccgc accaccgcgg cgcgggctca
     4201 ccacgtggca ggcgtggggc ctcatcctga tcgcgcccta cgcgatcgtg ttcctcgcgt
     4261 tcgtgctgta cccggtgagc tacggtctgt ggctcgcgcg ccacccggag agctacgcgc
     4321 ggctcttcgc cgatccgatt ttcgtgcgaa ccgcgatcaa caccgtcgtg ttcttgctgg
     4381 tggccgtaaa cctgaaattc ctgctcgcgc tctggctctc gggattcttc gtgcacgagc
     4441 gcgtgtggat ccgctggctc tcggtcgtct tcctgctgcc gtgggcggtg ccgtcgatcc
     4501 cgacgatctt ctccatccgc ttcatgttca atcccgagtg gggcgtggtg aactcggtga
     4561 tcttcaggct caccgcgcag gacgggccga actggctgaa cgatccgcat atcggcctcg
     4621 gcctcgcgat gctcacgcac atctggaagt cgctgccgtt ctggacgctg atcctcatgg
     4681 ccgggcggct cgcgatcccg cgcgagcagt acgaagccgc ggcggtcgac ggcgcgagcg
     4741 cctggcagaa gttcaagtac gtcacctggc cgagcctgcg cacgctttac ctcagctcga
     4801 cgatcctctc gatgatctgg tcgctcggcg acttcaacag cgtctacctg ctcaccggcg
     4861 gcgggccggc cgatctcacg cacgtcctcg ccacgctcgg catccgatat atccgcctgg
     4921 accaagtcga tctcgcgatg gcgacgatcg tcgtcgcgat gccgctcgtc ctgcctttgg
     4981 tctactacat gatgaagcac ctgtcgcgcg agtgaggaaa ttcacgcgcg aagcggtcct
     5041 cgtgctggtg gcg
//
LOCUS       DASTPD010000357         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_606431, whole genome shotgun sequence.
ACCESSION   DASTPD010000357 DASTPD010000000
VERSION     DASTPD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606431"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFR57_12065"
     CDS             <1..778
                     /locus_tag="VFR57_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260137.1"
                     /translation="PGAPNQPSLAWRYTTGAASGSVPFTIPATLAPGTYELRLFSNGG
                     YTRLAVSSSFTVPAVGCTGTSLSASPASVPVGGSITAAWSSICAPTGKDWVGLYLPGA
                     PDASYLAWRYTTGTASSSVPFTIPATLAPGTYELRLFSNGGYTRLAVSNSFTVTAVVG
                     CTGITLSASPASVPAGGSVTATWDGICAPTSTDWIGLYLPGTPNQPSLGWRYTTGTAS
                     GSVPFTIPASRAPGTYELRLFSNNGYTRLGTSNTIVVTSP"
     gene            1114..3216
                     /locus_tag="VFR57_12070"
     CDS             1114..3216
                     /locus_tag="VFR57_12070"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0260138.1"
                     /translation="MRRRAGSLVNGRTARRRRKHIPFPPLFKRADGSPMAAPPRTPLA
                     EGKVLYVGQPVVALVAETRAQAQDAAELVTIEYEELRCVVDPRKAVEPGAPLVWEQAS
                     GNVAAEAVYGDKEAADTAFFAAAHVTHIEFHNQRLNAMALEPRCAIGVHEGARTTLYT
                     QCQTPTAARELLGAVFGEPPANFRLLNGDIGGGFGMKTGLTPEDALVCYAARKLGRPV
                     KWRADRAEEFLAAHMARDQHYRAALALDREGRILALRMEMLANIGAVPVGSSAMIPLA
                     MVAKVATSAYRVPVVDYRIKGVLTNTMATGAYRGAGRPEGNYLIERLLEKTAREMRID
                     SVELRRRNLLRADELPYRTHVGETYDSGEFERMLDGALEAGDWNGFLERRKASSRLGK
                     LRGRGMSVYLEWTGALPTETVDIEIDADGVVTVFSGTQAMGQGLETTYTQLVTEILGI
                     GSDKIKVVQGDTDRANGVGSVGSRSAFVGGSAVVAAGRKMLSKLSELAAEKLEAASSD
                     IELRDGRLRIAGTDRSISLSELAGRQPTKLIRVAATETPSTPSWPNGAQVAEVEVDPD
                     TGEVTLAALTSCDDIGRIINHAIVEGQIHGGMAQGAGQALWEEVRYDEASGQLLSGSL
                     MDYCVPRADQLPPLAPHFDESVPCKTNLLGVKGCGELGTIGAIPAVVHAVLDALELPH
                     LEMPLTSEKVWQALRRGR"
     gene            3206..3898
                     /locus_tag="VFR57_12075"
     CDS             3206..3898
                     /locus_tag="VFR57_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0260139.1"
                     /translation="MDDSRAFHYALATSLALHALLLVAFPDLADTARRAASIPPQIIA
                     RLMEPEPAPPVPVPAPPVQTPTPPVPPEKKKAPAAKKPAPVISTPHPAPTPSVPAVAD
                     PVPAPPPPPLAAMDPKPPTPAAAPQPAQPPESLSRDQYRLQLIDEARRHKRYPPLARE
                     NNWQGDVRVDVAISANGRPTVTLKGSSGYEVLDRQALDMFAQAARSIPVPPALRGKEF
                     SFEVRAVYGLED"
     gene            complement(3950..4216)
                     /locus_tag="VFR57_12080"
     CDS             complement(3950..4216)
                     /locus_tag="VFR57_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0260140.1"
                     /translation="MLASKPYGTLYIGVTSNLAGRIEAHRNSSVDGFTKEYGVHTLVY
                     FEVHADMYEAIQREKRLKKWNRAWKIRLIEEMNPEWKDLSAQAF"
     gene            4097..>4387
                     /locus_tag="VFR57_12085"
     CDS             4097..>4387
                     /locus_tag="VFR57_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260141.1"
                     /translation="MHTVFLGKPVHAAVAMRFNATGEIARYAYVQRPVWFAGEDVDHG
                     LLHFATNALTRAVVDLKKLGPRLRGDDYRRFFEVSLGRLFRPERDHMVDVEPD"
BASE COUNT          747 a         1477 c         1422 g          741 t
ORIGIN      
        1 cccgggcgct cccaaccagc cttcgctcgc ttggcgctac accacgggcg cggcaagcgg
       61 cagcgtgccc tttacgatcc cggccacgct cgccccgggg acatatgagc tgcggctatt
      121 ctccaacggg ggatacacgc gcctcgcggt cagcagcagt ttcaccgttc cagcggtcgg
      181 ctgcacgggc accagcctca gcgcgagccc ggcgagcgtt cccgtcggtg gctcgatcac
      241 ggccgcctgg agcagcattt gtgcacccac cggtaaagac tgggtcggcc tctatctccc
      301 gggcgccccc gacgcgtctt acctggcgtg gcgttacacc acgggcacgg cgagcagcag
      361 cgtgcccttt acgatcccgg ccacgctcgc cccggggaca tatgagctgc ggctgttctc
      421 caacggggga tacacgcgcc tcgcggtcag caacagtttc acggttaccg cggttgtcgg
      481 ctgcacgggc attaccctca gcgcgagccc ggcgagcgtt cccgccggtg gctccgtgac
      541 ggccacctgg gacggcattt gtgcgcccac cagtaccgac tggattgggc tttatctccc
      601 gggcactccc aaccagcctt ccctggggtg gcgttacacc acgggcacgg caagcggcag
      661 cgtgcccttt acgatcccgg cctcgcgcgc cccggggacc tacgagctgc gcctgttctc
      721 caacaacggc tacacgcgcc tcggcacgag caatactatt gtggtcacct caccgtgaaa
      781 gcggcattgc gttcttgagt tcggatcgtt tggccgctta ccgctacgcg acttgaaatc
      841 tgttgtacgt tttgcggtac cgggggttcg aatccctctc tccgccagat ccctctagat
      901 gagcgcagtt ccgctgcagg tccagttcga tccggcgtcg gcgcgctttg gcgcgtcgcg
      961 ctcgcagaag cgcctcgagg acgaccggct tctcctcggc aaaggcctct tcagcgacga
     1021 ccgggtcttc ccgggcgagg catggatagt gctcgtaagg tcgccccatg ctcacgcccg
     1081 cattctcaat attgatattt cagaagcccg taaatgcgaa ggcgtgctgg cagcctggtc
     1141 aatggccgaa ctgcgcgccg acggcgtaag cacatcccgt ttccgccgct cttcaagcgc
     1201 gcggatggct cgccgatggc cgccccgccg cgcacgccgc tcgccgaagg gaaggtgctc
     1261 tacgtcggcc agccggtggt ggcgctcgtc gccgagacac gcgcgcaggc gcaggacgct
     1321 gccgagctcg tcacgatcga atatgaggag ctacgttgtg tcgtagatcc gcgcaaagca
     1381 gtcgagccgg gcgcaccgct tgtctgggag caggcaagcg gcaacgtcgc ggcggaagcg
     1441 gtttatggag acaaggaagc tgctgacacg gcgttcttcg cagccgcgca cgtcactcac
     1501 atcgagtttc acaaccagcg gctcaacgcc atggcgctcg agccgcgctg cgcgatcggg
     1561 gtgcacgaag gcgctcgcac gacgctctac acgcagtgcc agaccccgac cgcggcgcgc
     1621 gagctgctcg gcgcggtgtt cggcgagccg ccagcgaatt tccgcctgct gaacggcgac
     1681 atcggcggtg gcttcggcat gaagacaggc cttacgcccg aagacgcgct cgtgtgctat
     1741 gcagcgcgca agctcggccg cccggtgaaa tggcgcgccg accgcgccga ggagttcctc
     1801 gccgcgcaca tggcgcgcga ccagcactac cgcgcagcgc tcgcgctcga ccgcgagggg
     1861 cgcatcctgg cgctgcgcat ggaaatgctc gccaatatcg gcgcggtgcc ggtcggctcc
     1921 tccgccatga ttccgctcgc catggtggcg aaggtcgcga cctcggctta ccgcgtgccg
     1981 gtcgtcgact accgcataaa gggcgtgctc accaacacca tggcgaccgg cgcgtatcgc
     2041 ggcgcggggc ggccggaagg caactacctg atcgagcggc tactggaaaa aaccgcgcgt
     2101 gagatgcgca tcgattccgt agagctgcgc cggcgcaacc ttctcagggc cgacgagctg
     2161 ccgtaccgga cgcacgtagg cgaaacatac gacagcggcg agttcgagcg catgctggac
     2221 ggcgcactcg aggccggaga ctggaatggg tttctcgaga gaagaaaggc ttcatcccgc
     2281 cttggaaagc tgcgaggccg tggcatgtcc gtttatctgg aatggaccgg cgcgttgccc
     2341 acggagacgg tggacatcga gatcgacgcg gacggcgtgg tgacggtgtt cagcggcacg
     2401 caggcgatgg ggcaggggct cgaaacgaca tacacgcagc tcgttacgga aattttggga
     2461 attggttctg acaagataaa ggtcgtgcaa ggcgataccg accgggcgaa cggcgtcggc
     2521 agcgttggct cacgctcggc tttcgtcggc gggtcggcgg tggtcgcggc cgggcgcaag
     2581 atgctctcga agctgagcga gctggctgcc gagaagctgg aggcggcgag ctcggacatc
     2641 gagctgcgtg acggccgcct gcgcatcgcc gggaccgacc gctcgatctc actctcggag
     2701 ttggcagggc gtcagccgac gaaattgatt cgggtcgccg cgaccgagac gccgtcgacg
     2761 ccgtcatggc cgaatggagc gcaggtggcc gaggtcgagg tcgatcccga caccggcgag
     2821 gtgacgctcg ccgcgctcac gagctgcgac gacatcggcc gcatcatcaa ccatgcgatc
     2881 gtcgagggcc agatccacgg cggcatggcg cagggcgcgg gccaggcgct ctgggaagag
     2941 gtgcgctacg acgaggcgag cggccagctc ctgtccgggt cgctgatgga ttactgcgtg
     3001 ccgcgcgccg accagcttcc tccgctcgca ccgcacttcg atgagagcgt tccgtgcaag
     3061 acgaacctgc tcggcgtcaa gggctgcggc gagctcggca cgatcggcgc gattccggcg
     3121 gtagtgcatg cggtgctcga tgcgctcgag ctcccgcacc tggagatgcc gctcacctcc
     3181 gagaaggtgt ggcaggcgct gcgccgtgga cgatagccgg gcgttccatt acgcgctggc
     3241 gacatcgctc gcgctgcacg cgctcttgtt ggtcgcgttt cccgatctcg cggatacggc
     3301 gcgtcgagcg gcgagcattc cgccgcagat catcgcgcgc ctgatggagc cggagcccgc
     3361 gccgcccgtc ccggtaccgg cgcctccggt acagaccccg accccacctg tgcctccgga
     3421 gaagaagaaa gcgcctgcgg caaaaaaacc tgcgccggtg atctcgacgc cgcatcctgc
     3481 gccgacgccg agcgtgcctg cggtagcaga tccagtcccc gctccgccgc ccccgccgct
     3541 ggcggccatg gacccgaaac cccccacccc tgcagcggcg ccgcagccgg cgcagccacc
     3601 tgagtcgcta tcgcgcgatc aataccggtt acagctcatc gacgaggcgc ggcgccacaa
     3661 gcgctatccg ccgctcgcgc gcgagaacaa ctggcagggc gacgtgcgcg tcgacgttgc
     3721 catctccgct aacggccgcc caaccgtgac gctcaagggg agctcgggct acgaggtgct
     3781 cgaccgccag gcgctcgaca tgttcgctca ggccgcgcgc tcgatcccgg tgccgccggc
     3841 cctccgcggc aaggaatttt cgttcgaggt cagggccgtg tacggcctcg aggactaagc
     3901 atggcctgaa ggtcgtcccc gcgaaggcgg ggaccccgtg tagaagaaat tagaacgcct
     3961 gcgcagaaag atccttccac tccggattca tctcctcgat gagcctaatc ttccatgcgc
     4021 ggttccactt cttcagtctc ttctcccttt gaatcgcctc gtacatgtca gcgtggacct
     4081 cgaagtacac gagcgtatgc acaccgtatt ccttggtaaa cccgtccacg ctgctgttgc
     4141 gatgcgcttc aatgcgaccg gcgagattgc tcgttacgcc tatgtacagc gtcccgtatg
     4201 gtttgctggc gaggatgtag accacgggct gcttcacttc gcgaccaacg cacttacacg
     4261 agctgtggtt gacctcaaaa aactgggtcc ccgcctgcgc ggggacgact atcggcgctt
     4321 ttttgaagtg tccctaggcc gacttttccg gcctgaacgc gaccacatgg tcgacgtcga
     4381 gccggat
//
LOCUS       DASTPD010000358         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_403612, whole genome shotgun sequence.
ACCESSION   DASTPD010000358 DASTPD010000000
VERSION     DASTPD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403612"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFR57_12090"
     CDS             complement(<1..422)
                     /locus_tag="VFR57_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0260142.1"
                     /translation="MKKMLALAALVFASSVQAQAWPSKPVRVIVNVAPGGVADVTARV
                     LGARLQETLGQAFPVENRAGGDGYIGFDAVARSDPDGHTLLYSPGSSMMIAPHIVNRP
                     DLDPLKVLAPVAATGRVSLYVLVHPERPFKNLSEFLAYA"
     gene            complement(419..1192)
                     /locus_tag="VFR57_12095"
     CDS             complement(419..1192)
                     /locus_tag="VFR57_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0260143.1"
                     /translation="MIRTHYCIDGSGARWVTFVTGIANDLTMWDGQVPAFAADFRILR
                     YDLRGHGGSAATRGDYSVSLLVEDLLALLNQLRIQTTSLVGLGLGGAIAQAFAIEHPS
                     RVEGLIPCCCRARMVPDFAAMWHKLRGTVQQEGLEAIVEPTVQRWFSEEFKAAHPQVL
                     ENIRKMIRRTTLEGYMGVTAAFLGLDLERRLPEIRARTLYVSGAEDKLGGPPELMKGL
                     SEKVNGATHVSVPNAAHIANIQNPERFNQLLTDFLKREQ"
     gene            1210..2175
                     /locus_tag="VFR57_12100"
     CDS             1210..2175
                     /locus_tag="VFR57_12100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0260144.1"
                     /translation="MKVIALEEHYWDRELAGHFKERGPEMRIPALQERLFDLGALRLK
                     EMDEAGIDLQVLSHGAPATQRLDPQTAVPLARRVNDRLDEVIKANPARFAGFAVLPTA
                     EPKAAADELERTVTKLGFKGGMVHGLTAVDESLLFLDDKRFWPIFERAQALDIPLYMH
                     PALPHKAVIEAYYKDYLEQFPGLMTAAWGFTVEAATQGIRLVLSGVFEAYPRLKIILG
                     HLGESLPFSLWRIDMALARQGNRKTPFRDTFREHFWITTSGNFSTPALLCCVMEMGVD
                     RILFSVDYPFVPNGPGPKWMETVPLSSADKAKILHGNARRLLKLQ"
     gene            2195..2461
                     /locus_tag="VFR57_12105"
     CDS             2195..2461
                     /locus_tag="VFR57_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260145.1"
                     /translation="MRTGLFLLSGFLFLAASLIVGKLFSSIYPAGTTIAAALFLVVWL
                     LIAAVNMWLGVAKAGYSLAVELPIFALIFGLPAIVAVLVRWKLL"
     gene            2653..3651
                     /locus_tag="VFR57_12110"
     CDS             2653..3651
                     /locus_tag="VFR57_12110"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260146.1"
                     /translation="MRGRIPLLTAIVFLMPGMLVASLPAMAQAPQKVFRVGHLAAPGK
                     THDGGPPRALVDALRGLGYVEGRNIAYEARFAEGKLERLPQLAAELVQLKMDVIVAQG
                     GASTEAARRATTTIPIVMAPAAGDAVAVGWISTLARPGGNITGLSDESVQLSAKRMEL
                     LKEAVPQAALIAIVWNDNDHGMTLRYREIEKAARTLRVEVLPLGVRNVEDFTGAFEAM
                     TRRKPDAMFIVTDVLTNMNRKRFVEFAATHRIPAMYETSTIVREGGLMSYGPSFEDGF
                     REAAGYIDRILKGAKPIDLPAVQPRRYYLAVNLKAAAALGLTMPPSLLLRSDELIQ"
BASE COUNT          676 a         1264 c         1277 g          737 t
ORIGIN      
        1 gcgtaggcga ggaactcgga gaggtttttg aagggccgct ccgggtgtac aagcacgtaa
       61 agcgacacgc ggcccgtggc cgccaccggc gcgaggacct tgagaggatc gaggtcaggc
      121 cggttcacaa tgtgaggcgc gatcatcatg ctcgagcccg gcgagtagag cagcgtgtgg
      181 ccgtccgggt cggagcgcgc gacggcgtcg aagccgatgt agccgtcgcc gcccgcacgg
      241 ttctccaccg ggaacgcctg tccgagcgtc tcttgcaggc gcgcgccgag cacgcgcgcg
      301 gtcacgtcgg cgacgccacc cggcgcgaca ttcacgatca cgcgtaccgg tttcgacggc
      361 cacgcctgcg cctgcacgga agacgcgaac acgagcgcgg cgagcgcgag catttttttc
      421 attgttccct cttcagaaaa tccgtgagca gctggttgaa tcgttccggg ttctggatgt
      481 tagcgatgtg cgcggcgttc ggcaccgaga cgtgcgtcgc gccgttcacc ttctcgctca
      541 atcccttcat gagctcgggc gggccgccga gcttgtcctc ggctccgctg acatagaggg
      601 tgcgggctct tatctcgggc aatcggcgtt ccagatcgag gccgagaaag gccgccgtga
      661 cccccatgta tccctcgagc gtcgtgcggc ggatcatctt gcgaatattc tccagcacct
      721 gcgggtgcgc ggccttgaat tcctccgaga accagcgctg cacggtcggc tcgacgatcg
      781 cctcgagtcc ctcctgctgc accgtgccgc gcagcttgtg ccacatcgcc gcgaagtcgg
      841 gaaccatgcg cgcgcggcaa cagcacggga tgaggccctc gacccggctt gggtgttcga
      901 tcgcaaacgc ctgcgcgatc gcgccgccga ggccgaggcc tacgagggaa gttgtctgta
      961 ttctcaattg gttcagaaga gcaagcaggt cctcgaccag gagcgagacg ctgtagtcgc
     1021 cccgcgtcgc cgcgctgccc ccatggccgc gcaggtcgta gcgcaggata cggaaatcgg
     1081 ctgcgaacgc cgggacctgc ccgtcccaca tcgtgaggtc gttggcaatg ccggtgacga
     1141 aggtcaccca gcgcgcgccg ctgccatcga tgcagtaatg ggtcctgatc aaggtaagat
     1201 gattttgcta tgaaggtcat cgcgctggaa gagcactact gggacaggga gctcgcgggt
     1261 cacttcaagg agcgcggccc ggagatgcgc atccctgcgc tgcaggagcg cctcttcgac
     1321 ctcggcgcgc tgcgcctgaa ggaaatggat gaggcgggca tcgacctcca ggtgctgtcg
     1381 cacggcgcgc cggccacgca gcgcctcgat ccgcagaccg ccgttccgct cgcgcggcgc
     1441 gtcaacgacc ggctcgacga agtcatcaag gcgaatcccg cccggttcgc cggttttgcc
     1501 gtcctgccta ccgcggagcc gaaagccgcc gcggacgagc tcgagcgcac ggtgacgaag
     1561 ctcggcttca agggcggcat ggtgcacggc ctcactgcag tcgacgaatc gctgctattc
     1621 ctcgacgaca agcgtttctg gccgatcttc gagcgtgccc aggcgctcga cataccgctc
     1681 tacatgcatc cggcgctccc gcacaaggcg gtgatcgagg cctactacaa ggattacctc
     1741 gagcagttcc cggggctgat gaccgccgcc tggggcttta ccgtcgaggc cgccacgcaa
     1801 ggcatccgcc tcgttctttc aggcgtgttc gaggcctatc cgcggctcaa gatcattctc
     1861 ggccacctcg gcgagtcgct cccgttctcg ctgtggcgca tcgacatggc gctcgcgcgc
     1921 cagggcaatc gcaagactcc gtttcgcgac accttccgcg agcatttctg gatcaccacg
     1981 agcggcaact tctcgacgcc agcccttctc tgctgcgtca tggagatggg cgtcgatcgc
     2041 atcctgttct ccgtagacta ccccttcgta cccaacggcc ctggcccgaa gtggatggaa
     2101 accgtgccgc tcagcagcgc ggacaaggcc aagatcctgc acggcaacgc gcggcggctg
     2161 ctcaagcttc agtagaatga ggtgagggta gcacatgcgt accggcctat tcctgctgtc
     2221 cggttttctt tttctcgctg catcgctcat tgtcggcaag ctcttctcct cgatctatcc
     2281 ggccggcacg actatcgcgg ccgcgctttt cctggtggtg tggctcctaa tagcagccgt
     2341 caacatgtgg cttggtgttg ccaaggcagg gtactcgctc gccgtggaac tgccgatctt
     2401 tgcgctgata ttcggcctgc ccgcgatcgt ggccgtgctg gtgcgctgga agttgctgtg
     2461 aaacggcgtg cacgctaagt cgggcaatcc catgtacgtc cccgtattgc tcatgctcgc
     2521 ggccgtcgtc gccgctgcgt tccatttcag agtcgtgctc aaggaagagc cgtagctcgc
     2581 tcgcacctgc gaaatctttt gtcccccttg ccttttgttg tcaccgtgcg ctaccgaagg
     2641 agggatagct ctatgcgtgg tcgcatcccg ctccttacgg ccatcgtttt cctgatgcct
     2701 ggcatgctgg tcgcgtcctt gccggccatg gcccaggcgc ctcagaaggt gtttcgcgtc
     2761 ggtcacttgg cggcgccagg caagacgcac gatggcgggc cgccgcgtgc gctcgtcgac
     2821 gcgctaagag ggctcgggta cgtcgagggt cgcaacatcg cgtacgaggc gcgctttgcc
     2881 gagggcaagc tggagcggct gccgcaactc gcggccgagc tcgtccagct caagatggac
     2941 gttatcgtgg cgcaaggcgg cgcgtcgacg gaggcggcga ggcgcgcgac gacgaccatt
     3001 ccgatcgtca tggcgcctgc tgctggcgac gcggtcgcgg tcgggtggat ctcgacgctt
     3061 gcgcggcccg gtggaaacat caccgggctg agcgacgaat ccgtccagct cagcgcgaag
     3121 cgcatggagc ttctcaagga agccgtgccg caggcagcct tgatcgcaat cgtctggaac
     3181 gacaacgatc acggcatgac cctgcgttac cgtgagatcg agaaggccgc gcgcaccttg
     3241 cgtgtggagg tgctgccgct cggggtgcgc aacgtcgagg atttcaccgg agccttcgaa
     3301 gcgatgacgc gtcgcaagcc ggatgccatg ttcatagtta cggacgtgct aacgaacatg
     3361 aatcgcaagc gattcgtcga gttcgcggcg acccaccgca tcccggcaat gtacgagacc
     3421 agcaccatcg tccgcgaggg cgggctgatg tcgtacgggc cgagcttcga ggacggcttt
     3481 cgcgaggccg ccggctacat cgaccggatt ctcaaaggcg cgaaaccgat cgacctgccg
     3541 gccgtgcaac cgagaaggta ctaccttgcg gtcaatctca aggcggcggc tgctttgggc
     3601 ctcacaatgc ccccgtcgct gctcctgagg agtgacgaat tgatccagta gcgatgcggc
     3661 agccggcggg tacgtatgcc gaagcggatc tcgagcggat catcgcgcgc gacttcgcac
     3721 aggactccat tcccgcggtc aaagcgttgc tatcgcgcta tgggaaggaa tcctggcagc
     3781 ggcaggtgtt gcgggttcag atggcatgct tgaagtgcgc caacggcgat ctcgaggcgc
     3841 tgcaaagagc gcgctccgcg ttaggcgcgg tttatcgcgg ctgcgatatg ctcgggtaca
     3901 agcggtagat cgcgcacgcg cacgtcgagc gcctcgtaga gcgcattggc gatc
//
LOCUS       DASTPD010000359         6981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1833528, whole genome shotgun sequence.
ACCESSION   DASTPD010000359 DASTPD010000000
VERSION     DASTPD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833528"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..750
                     /locus_tag="VFR57_12115"
     CDS             136..750
                     /locus_tag="VFR57_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0260147.1"
                     /translation="MSIALIGGAGEGLGAALGRRFSKTHEVVLSSRTRGEKADLREEK
                     QVIDLFERVEQRGAIEVAIFNAGAHFRGSIVDTPAATFEEVWRLACYAGFLFGREAAR
                     RMVARGKGTILFTGATASIRGSANFAAFAAAKGGLRQVAQSMARELGPKGIHVGHIII
                     DGRIGHGDNMLAPDAIADLYWQLHAQPRSAWTFEADLRPWSEKF"
     gene            complement(781..1644)
                     /locus_tag="VFR57_12120"
     CDS             complement(781..1644)
                     /locus_tag="VFR57_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0260148.1"
                     /translation="MLYVKLVLMALFWSGVFPAINIVLSSMGLFTSVFLRFACAALIL
                     LGLLRLREPRMPRPSPRETVLIIGLGILGISVYNVLFSAGLQLVEASRAALIVPTNPA
                     FTALFAAIFLKERLSRTRAAGIALCVLGALWVLSRGDLRLLATLDLGLGEALLVLCIF
                     MWSAYTLLGRVALSGLPALALTAYVMTAGAVMLAIPAAIEHAPLAGVTWQAWVALGYL
                     IVFGSVLPFLWFYEGVKALGAARASQFINLVPPLAVAESVLILGEPMTAALYVGTALV
                     LAGLYLTNRRR"
     gene            1673..2053
                     /locus_tag="VFR57_12125"
     CDS             1673..2053
                     /locus_tag="VFR57_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260149.1"
                     /translation="MRHFVAVALALAPLTASANDIEIQRSVIQRDQQSAEFAARVRGE
                     DTSRLERLHARQLSEVYRPPLNPDPEVARQLQPYQRQKMADERTLVLPPPTATLRKPL
                     EKPLPLPGGPRPGVELVPPQSPPN"
     gene            complement(1988..4600)
                     /gene="mdeB"
                     /locus_tag="VFR57_12130"
     CDS             complement(1988..4600)
                     /gene="mdeB"
                     /locus_tag="VFR57_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HEU0260150.1"
                     /translation="MTDLADIVSSAFWRVNPPDADPAETREWLEAFDALVAAEGRERA
                     TFLLRKLLDHARARRVPLPPVLNTPYKNSVSLAHQPQFPGNLELESRISAIVRWNALA
                     MVVRANRAHPELGGHIASYASAADLFEVGFNHFFRADDLVYFQPHSAPGVYARAYLEG
                     RLSEERLAHYRQETGGKGLSSYCHPYLMPEFWQFPTGSMGLGPINAVYQARFMRYLEN
                     RNLATTQSRKVWSFVGDGEMDEPESLAGLSLATREGLDNLIFVVNCNLQRLDGPVRGN
                     GSIVQELEGLFAGAGWNVIKLLWGSDWDPLFARDEDGVLLKRLHETVDGEFQTYAATD
                     GRFNREHFFNKYPELRALVADMSDDDIDRLRRGGHDPVKIYAAYHAAAHHKGQPTVIL
                     AKTKKGYGMGLWGQGKMGTHQQKKLDDEALREFRNRFALPLSDEDVAQLRFYHPGPQS
                     PEIKYLLERRQALGGFVPTRTTKSEKLPVPSLGATERNQSTTMAFVQLLSQLLKDEKL
                     GPRVVPIVADEARTFGMQTLFRQVGIYSAVGQLYEPEDHDELLYYREAKDGQILEEGI
                     TEAGALSSWLAAATSYSAHGKPMLPFYIYYSIFGFQRVGDLIWAAADSRARGFLVGGT
                     AGRTTLAGEGLQHQDGSSHLIAATFPNCRAYDPCFAYELGAIVEDGALRMLEAQEDVF
                     YYLTVANENYPHPAMPKDAREGILRGMYRIREGAQVQLLGSGPILREVLAAAELLQKD
                     WNIAAGVWSVTSFTELRRDGMAAERTRRLGGQAKSWVETCLGGTDGPVVAASDYVRAV
                     PDLIRGFVKSPYTVLGTDGFGRSDTRAALRAFFEVDARHIAIAALSAIDASLVRGALR
                     RYELDPGARPPWER"
     gene            4841..5044
                     /locus_tag="VFR57_12135"
     CDS             4841..5044
                     /locus_tag="VFR57_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0260151.1"
                     /translation="MATGTVKWFNDSKGFGFITPSEGGEDLFAHHTAIQMSGFKTLKE
                     GQKVEFEVKRGPKGLQAHNIKPL"
     gene            complement(5089..6897)
                     /gene="glsA"
                     /locus_tag="VFR57_12140"
     CDS             complement(5089..6897)
                     /gene="glsA"
                     /locus_tag="VFR57_12140"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEU0260152.1"
                     /translation="MSASPILEYLESLRARFVGLTDGKVAAYIPELSHADPDWFGICI
                     ATRDGFVYEVGDSRQPFTLQSISKPLTYGLVLEENGEDTVLAKIGVEPSGEAFNAISL
                     RPQTGAPLNPLINAGAIAACGLIQGDAAEAKIARVLGAFSRYAGRALDIDERVYNSES
                     ATGHRNRAIGWMLRNFAILGNEPSPTLETYFRQCSIRVTCRDLALMGATLANGGVNPV
                     SGVRALAEEYVDNVLSVMATCGMYDYSGEWLYRTGLPAKSGVGGGILAVQPGRLGIGV
                     FSPRLDPQGNSVRGIAVCRALAAELGLHLFDAAQPRAPAVRLATTRRKVASKQRRPPA
                     VAEHLRKAGKRLRLYHLQGPLAFAAVEPVVREIMARASDTNCFILNLRMVQAIDPAAT
                     SLLAGTRRELLGLGKVLIFSEASTWWQLLIDAGIDREAIFTDDDFALEYGENLLLAEC
                     FQDVAWEAQTPLEKCALFAGFEFEELALLERLLATRCYQRGQTIVAAGQSSDELFVIT
                     RGSAMVSVPTQNGVTRLNSFTSGMTFGDIAFIDRSPRSANVTALGPVECRVLTRQAFT
                     RLDSEAPAVKIRLLQNLAHGMSGMVRHLSRELAALK"
BASE COUNT         1270 a         2243 c         2332 g         1136 t
ORIGIN      
        1 cgatacggtc gtcgtttgcg ccgggcagac gccgcggcgc ggtgtctcag gtcacttgat
       61 cggcggcgcc aacgaagcct ccgagctcga cgccaagcgc gcgatgctcg agggcgccga
      121 gctggcggcg cggctatgag catcgcgctc atcggtggcg cgggcgaagg cttaggcgcg
      181 gcgctcggcc gccgtttctc caagacgcac gaggtcgtgt tgtcttcgcg cacgcgcggc
      241 gaaaaggccg acctgcgcga ggagaagcag gtcatcgatc tcttcgagcg cgtggagcag
      301 cgcggtgcga tcgaggtggc gatcttcaac gccggcgcgc acttcagggg ctcgatcgtc
      361 gacacccccg ccgcaacctt cgaggaggta tggcgcctcg cctgctacgc cggctttctc
      421 ttcggccgcg aagccgcgcg ccgcatggta gcgagaggca aaggcaccat cctcttcacc
      481 ggagcgaccg cctcgattcg tggctcggcc aacttcgccg cgtttgccgc cgcgaagggc
      541 ggcttgcgac aggtcgcgca gtccatggcg cgcgagctcg ggccgaaggg cattcatgtc
      601 ggacacatca tcatcgacgg ccggatcggt catggggaca acatgcttgc ccctgacgcg
      661 attgccgacc tctattggca gcttcacgcg cagccgcgct cggcgtggac cttcgaagcc
      721 gatctgcgtc cctggtcgga gaagttctag aaaagaatcg ggctctgacc ccgatttttc
      781 ctagcggcgg cggttggtga ggtagaggcc ggcgagcacg agcgcggtgc cgacgtagag
      841 cgctgcggtc atcggctcac cgaggatcag caccgactcg gccaccgcga gcggcggtac
      901 caggttgatg aactgagagg cgcgcgcggc gccgagcgcc ttcacgccct cgtagaacca
      961 caagaagggc agcaccgagc cgaagacgat taggtagccg agcgccaccc atgcctgcca
     1021 cgtcacaccg gcaagcggcg cgtgctcgat cgccgccgga atcgcgagca tcacggcgcc
     1081 ggcggtcatg acataggctg tgagcgcgag cgcaggcaag ccggaaagcg cgacgcgtcc
     1141 gagcagcgtg tacgcgctcc acatgaagat gcagagcacg agcagcgctt cgccgaggcc
     1201 gaggtcgagc gtggcgagca gccggaggtc gccgcgcgaa agtacccaga gtgcaccgag
     1261 gacgcacagc gcgatgcccg cggcgcgcgt gcgactcagg cgctctttga gaaaaatcgc
     1321 ggcgaagagc gcggtgaacg ccggattcgt cggcacgatg agtgccgcgc ggcttgcttc
     1381 gacaagctgc aagcccgcgc tgaagagcac gttgtagacg ctgatgccga ggatgccgag
     1441 tccgatgatc agaacggtct cgcgcggcga tggtcgcggc atacgcggct cgcgcagccg
     1501 aagcagcccg aggaggatga gcgcggcgca cgcgaagcgc agaaacaccg aggtgaagag
     1561 gcccatgctc gagagcacga tgttgatggc ggggaagacg ccgctccaga agagcgccat
     1621 cagcacgagc tttacgtaaa gcatcggggc gccgcatgct agcatcggag cgatgaggca
     1681 cttcgtcgcg gtcgctctcg cgctcgcccc gctcaccgcg tcggctaacg acatcgagat
     1741 ccagcgctcg gtgattcagc gcgaccagca gtcggcggag ttcgccgcgc gcgtgcgcgg
     1801 ggaagacacc tcgcggctcg agcggctgca cgctcgccag ctctccgagg tctatcggcc
     1861 gccgctcaat ccggatcccg aagtcgcgcg ccagctgcag ccttatcagc gccagaagat
     1921 ggcggacgag cgcacgctgg tgttgccacc gccgaccgcg acgctcagaa aaccgctcga
     1981 aaagccgcta ccgctcccag gggggccgcg ccccggggtc gagctcgtac cgccgcagag
     2041 ccccccgaac taggctcgcg tcgatcgccg acagcgcggc gatcgcgatg tggcgcgcat
     2101 cgacttcaaa aaaggcgcgc aacgccgcgc gcgtatcgct ccggccgaag ccgtcggtgc
     2161 ccagcaccgt gtacgggctt tttacgaatc cccgaatcaa gtccggaacg gcgcgaacgt
     2221 agtcgctcgc ggcgaccacc ggtccgtctg ttccgccgag gcatgtctcg acccagctct
     2281 tagcctgtcc gccaaggcgg cgcgtgcgct cggcggccat gccgtcgcgc cgcagctcgg
     2341 tgaacgaggt cacgctccag acgcccgcgg cgatgttcca gtccttttgc aggagctcgg
     2401 ccgccgcgag cacttcgcgc agaatcggac cggaaccgag gagctgcacc tgcgcgcctt
     2461 cgcgaatgcg gtacatgccg cgcaagattc cctcacgcgc gtccttcggc atagcggggt
     2521 gaggatagtt ttcgtttgcg acggtgaggt aatagaacac gtcctcctgc gcttcgagca
     2581 tgcgaagcgc cccgtcctct acgatcgcgc cgagctcgta ggcgaagcac gggtcgtacg
     2641 cccggcaatt cgggaatgtc gcggcgatga gatgcgacga gccgtcctgg tgctggagcc
     2701 cttcgccggc aagcgtggtg cggccggccg tgccgccgac cagaaaacca cgcgcacgcg
     2761 agtcggccgc ggcccagatg agatcgccga cgcgctggaa gccgaagatg gagtagtaga
     2821 tgtaaaacgg aagcatgggc ttgccgtgcg ccgaatacga ggtcgccgcg gcgagccacg
     2881 aagaaagcgc gccggcctcg gtgatccctt cctccaggat ctgaccgtct ttcgcttcgc
     2941 ggtaataaag aagctcgtcg tggtcttcgg gctcgtagag ctggccgacc gcggaataga
     3001 tgccgacctg ccggaacagc gtctgcatac cgaacgtgcg cgcctcgtcg gcgacgatcg
     3061 gcacgacgcg cggtccaagc ttctcgtcct tgaggagctg cgaaagaagc tgcacgaacg
     3121 ccatcgtcgt ggactggttg cgctcggtag cgccgagcga cggcacgggc agtttctcgc
     3181 tcttcgtcgt cctggtcgga acaaagccac ccagcgcctg gcggcgctcg aggaggtatt
     3241 tgatctcggg gctctgcggc ccggggtggt agaagcgaag ctgcgccacg tcctcgtcgg
     3301 aaagcggcag cgcgaagcgg ttgcggaact cgcgcaaggc ttcgtcgtcg agcttcttct
     3361 gctggtgggt gcccatcttg ccctggcccc aaagacccat gccgtagccc ttcttcgtct
     3421 tcgctaggat caccgtgggc tggcccttgt gatgcgcggc ggcgtggtag gcggcgtaga
     3481 tcttcaccgg atcgtgcccg ccgcggcgca ggcgatcgat gtcgtcgtcg ctcatgtcgg
     3541 cgacgagcgc gcgcagctcg gggtacttgt tgaagaagtg ctcgcggttg aagcggccgt
     3601 cggtggcggc ataggtctgg aactcgccgt cgacggtctc gtgcagccgc ttcagcagca
     3661 cgccgtcttc gtcgcgcgcg aagagcggat cccagtccga gccccacagg agcttgatca
     3721 cgttccagcc ggccccggcg aagaggccct cgagctcctg cacaatcgag ccgttgccgc
     3781 gcaccgggcc gtccaggcgc tgaaggttgc agttaacaac gaagatcagg ttgtcgaggc
     3841 cttcgcgggt ggcgagcgaa aggccggcga gcgattcggg ctcgtccatc tccccatcgc
     3901 cgacgaagct ccacaccttc cggctctgcg tcgtggcgag gttccggttt tcgaggtagc
     3961 gcatgaagcg cgcctgatag accgcgttga tcggacctaa tcccattgaa ccggtgggga
     4021 actgccagaa ctcgggcatg agatacggat ggcaatagga cgacaggcct ttgcccccgg
     4081 tttcctgccg gtagtgagcc agccgctctt cgctcagtcg cccctcgagg taagcccgcg
     4141 catacacgcc gggcgccgaa tggggctgga agtacacgag gtcatcggct cggaagaagt
     4201 ggttgaaacc gacctcgaag agatcggctg ctgacgcgta gctcgcgatg tgaccgccga
     4261 gctcgggatg cgcacggtta gcacgcacta ccattgcgag cgcgttccag cgcacgatcg
     4321 cagagatacg gctctcgagc tcgaggttgc cggggaattg cggctggtgc gcaagcgaga
     4381 ccgagttctt gtagggcgtg ttcaggaccg gcggcagcgg cacacgccgc gcgcgcgcgt
     4441 gatcgagcag cttccgaaga aggaaagtcg cgcgctcgcg accctcggcg gcgaccaggg
     4501 catcgaaggc ttcgagccac tcgcgggttt cggcgggatc ggcgtcgggc gggttcacgc
     4561 gccagaacgc ggaggaaacg atgtcggcga ggtcggtcat gcggtcatgg taataggccg
     4621 cccggggggt tgctatcccc gtgcttctac gggaaaaacg gtgttatgat ccgcgccgca
     4681 ggatgtgttt caagcagcga atctgtgccg tccattcacc cgccgccgca atagcaggct
     4741 cgcgcaggtc cgtagtggca gtacgttccc acttgatacc ccaccgcccg gcttcgtcgc
     4801 gcccggattc gtcgcggctt tcgtagatag aggtaagtaa atggcaaccg gtaccgttaa
     4861 gtggttcaac gactccaagg gcttcggctt catcaccccg agcgaaggcg gcgaggacct
     4921 gttcgcgcat cacaccgcga tccagatgtc cggcttcaag accctgaaag agggccagaa
     4981 ggtcgagttc gaagtgaagc gcggccccaa gggtctgcag gcgcacaaca tcaagcccct
     5041 gtaagagccg caaggcgcga aatcaaagcc cgctttcggc gggctttttc atttcaacgc
     5101 agcaagctcg cggctgagat gccgcaccat cccgctcatc ccgtgcgcca ggttctgcaa
     5161 cagccggatc ttgaccgccg gggcttccga gtcgaggcgg gtaaaggcct ggcgcgtaag
     5221 cacgcggcac tccaccgggc cgagggcggt cacgttcgcc gagcgcggcg agcggtcaat
     5281 gaaagcgata tcgccgaatg tcatgcccga ggtaaaggag ttgagccggg tgacgccatt
     5341 ctgtgtcggg acactcacca tcgctgagcc gcgggtaatc acgaaaagtt cgtccgagct
     5401 ctgcccggct gccacgatcg tctgcccgcg ttgatagcat cgggtggcga gcagccgttc
     5461 gagcagggcg agctcctcga actcaaagcc ggcaaacagc gcgcattttt caagcggcgt
     5521 ttgcgcttcc cacgccacat cctggaagca ctcggcaagc agcagattct caccgtactc
     5581 gagcgcaaag tcgtcatcgg tgaagatcgc ttcacgatcg atcccggcgt cgatcaggag
     5641 ctgccaccac gtgctcgcct ccgagaagat gagcaccttg cccaggccga gcagctccct
     5701 gcgggtaccg gcaaggagac ttgtcgcggc cggatcgatc gcctgcacca tcctcaaatt
     5761 gaggatgaag caattggtgt cgctcgcgcg cgccattatc tcgcgtacta cgggctcgac
     5821 cgcggcgaag gcaagcgggc cctgcaggtg gtacaagcgc agtcgcttgc ccgctttgcg
     5881 caggtgctcg gcgacggcgg gcggacggcg ctgcttggat gcgaccttcc ggcgcgtcgt
     5941 agctagacgc actgccggcg cgcgcggctg ggccgcgtcg aacagatgca gcccgagctc
     6001 tgcggcgagc gcgcggcaca cggcaatgcc gcgaacgcta tttccctgcg ggtcgagacg
     6061 aggggaaaac acgccgatgc cgagcctgcc tggctggacg gcgagaatcc cgccgccgac
     6121 gccgctcttc gccggcaagc ccgtgcggta cagccattcg ccggagtaat cgtacatacc
     6181 gcaagtcgcc atcaccgaca aaacgttgtc cacgtattcc tcggcgagcg cccgaacgcc
     6241 cgacacgggg ttgacaccgc cgttggcgag cgtcgctccc atgagggcca agtcgcgaca
     6301 cgtaacgcga atcgagcatt gccggaagta ggtctcgagc gtcggcgacg gctcgttgcc
     6361 gagaatcgcg aaattccgca gcatccagcc gatcgcccga ttcctatgac ccgtggcact
     6421 ctcggagttg tagacgcgct cgtctatgtc gagcgcgcgg cctgcgtagc gcgagaacgc
     6481 cccgagcacg cgtgcaatct tcgcctccgc cgcgtcaccc tggatcagcc cgcaggcggc
     6541 gatcgcacca gcgttgatca gcgggttcag cggcgcacca gtttggggcc gtaggctgat
     6601 cgcgttgaac gcctcgcccg acggctcgac gccgatcttg gcgagcacgg tgtcctcgcc
     6661 gttctcctcg agcacgagcc cgtacgtgag cggcttggag atcgactgca aggtaaaggg
     6721 ctgccgcgag tcacctactt cgtagacgaa cccgtcgcga gtggcgatgc agataccgaa
     6781 ccagtccggg tcggcgtgcg agagctcggg gatgtaggcg gcgaccttgc cgtcggtcaa
     6841 gccgacgaag cgggcgcgca gcgactcgag gtactcgagg attggcgagg cggacacaac
     6901 ggatttctaa ttggagttct cgtcagtttc gcaagaactg tgcccgcgaa ggccgacgat
     6961 tacgcggttc cttcgttcct t
//
LOCUS       DASTPD010000360        14411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_404051, whole genome shotgun sequence.
ACCESSION   DASTPD010000360 DASTPD010000000
VERSION     DASTPD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404051"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFR57_12145"
     CDS             complement(<1..141)
                     /locus_tag="VFR57_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0260153.1"
                     /translation="MAKSKFERTKPHVNVGTIGHVDHGKTTLTAAMTHILAKKYGGEA
                     KNY"
     gene            complement(153..2255)
                     /gene="fusA"
                     /locus_tag="VFR57_12150"
     CDS             complement(153..2255)
                     /gene="fusA"
                     /locus_tag="VFR57_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0260154.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDLSYPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACMVYDAVAGVQPQSETVWRQANKYRVPRLAFINKMDRVGATFFKSYEHIRT
                     RLKGNPVPIQLPIGAEEKFAGVIDLVNMQAIYWDDATQGMKFERRPIPAELAAEAKEW
                     RDKMVEAAAEANEELMNKYLESGELSVEEIKKGIRIRTINNEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPIDVPPVKGENQKGEPEFRKPGDDEPFAALAFKIMTDPFVGQLTFI
                     RVYSGVLTSGDTVYTSMKGKKERIGRLLQMHANEREEIKEVRAGDIAAVVGLKEVVTG
                     ETISDVKNVITLERMEFPEPVIAQAVEPKTKVDQEKMGMALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEASVGKPQVAYRETFRKAIESEGKFVKQSGG
                     RGQYGHVWLKLEPQVQGKGYEFVDAIKGGRVPKEYIPAVEKGVNEAMQDGVLAGYPVV
                     DVKVSLFDGSYHEVDSNENAFKMAAIFAFKDGMRSAQPVLLEPIMAVEVETPEEKMGD
                     VIGDLSGRRGVIQGMEDMPGSKAIRAEVPLGEMFGYSTTLRSLTQGRATYTMEFKHYA
                     EAPKSVADAIMKKDEEKK"
     gene            complement(2262..2732)
                     /gene="rpsG"
                     /locus_tag="VFR57_12155"
     CDS             complement(2262..2732)
                     /gene="rpsG"
                     /locus_tag="VFR57_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU0260155.1"
                     /translation="MPRRREVPKREILPDPKFNNVEVAKFVNVLMTRGKKSVAERIIY
                     GALETIKSKSGKDPIEVFTQAVQNVKPVVEVKSRRVGGANYQVPVEVRPVRRMALAMR
                     WIREAAQKRGEKSMTVRLAGELQEAAEGRGGAMKKREEVHRMAEANKAFSHYRF"
     gene            complement(2742..3119)
                     /gene="rpsL"
                     /locus_tag="VFR57_12160"
     CDS             complement(2742..3119)
                     /gene="rpsL"
                     /locus_tag="VFR57_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU0260156.1"
                     /translation="MPTVNQLVRDGRAAKRAKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQGRSKYGAKAPKSA"
     gene            complement(3243..3701)
                     /locus_tag="VFR57_12165"
     CDS             complement(3243..3701)
                     /locus_tag="VFR57_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEU0260157.1"
                     /translation="MDEIVARSLAKWPNVPAVYGWLELDRRGNWLIKGERIGNAALRE
                     FIGRNYEADERGRWFFQNGPQRVYVTLAYTPLVVHYEGDRLYDHCGRPFVCRNAFQDD
                     EGSVLLEGERGIALLDDRDLARYADSAQALPRIRRSEVETRFNFFSDPGI"
     gene            3745..5406
                     /locus_tag="VFR57_12170"
     CDS             3745..5406
                     /locus_tag="VFR57_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU0260158.1"
                     /translation="MSAIETYLAKPWLAHYQKGVPPTVEVPLKSVAQAFDEATERAPE
                     RPALVFYGRSIAYRELREATDRLACALADLGVRKGDRVALYLLNSPQFIIAYFAALKC
                     GATVTPISPVYTSHEVGYQLKDSGARAIICQDILHEKVVKCGAALDLVVVTNVNEYLP
                     ALKRLFGKKAEVTGRVHWLQDLLKKYPAKPPAVTIDPKKDLAALPYTGGTTGSPKGVM
                     LTHYNLVAAQAMGQSAFPVFEQGKEVILAFLPFFHIYGQVVIMLQGLCQGHLLVLFTS
                     PDTEAILDAMERYGATVFYGVPTLYEYLKDHKDTNKANWRRLKLIVSGADTLHESTTQ
                     GWTRRTGSTITEGYGLSETCATSHINPLQRPKAGSFGCPITSMQAAVIDPESLEFVPA
                     NETGELVLCGPNVMQGYWQRAEDTARAFVDKGGVRWMRTGDLVRMDDEGYFHFYDRSK
                     DLIKYKGYSIFAKDVEDVLYAHPQVKAAGVIGVPDPAVGQRIKAIVVLQGDARGKLSE
                     DEIKAYCRQSLAEYKVPHIVEFRGELPKTDVGKVSRRELRDETKV"
     gene            5409..6182
                     /locus_tag="VFR57_12175"
     CDS             5409..6182
                     /locus_tag="VFR57_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0260159.1"
                     /translation="MAGPFFAVETLTKRFGGLTAVNNVSFSLRRDEIVGLIGPNGAGK
                     TTLLRLITKILKPDAGRIVFNGENITALRPWDVVERGIAGTFQNTRPFRHLPIVANVM
                     VPLVAPRARRGGEWVKRIEAKAMDALEFVGISDMALEPASALSQGDLKRLEVARAIAT
                     EPELLLLDEPLGGLNPAESELLANSIRRLHKGGRFGRLHSEGPAVLMIEHKLKELMSI
                     VDRVIVIDYGEIIADAPPAEVVKNPKVIEAYLGSEHAAA"
     gene            6169..6918
                     /locus_tag="VFR57_12180"
     CDS             6169..6918
                     /locus_tag="VFR57_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0260160.1"
                     /translation="MPLLEVRNLRVRYDKAQILNGVTLSVNEGELVGLVGPNGAGKST
                     LLRAISGLVHFEERMKRGTHGDIVLEGEVRFAGERIDGLAAHEIRMRGLVHCPERRRP
                     FRELTVQENLLAGAFLSTSKEQTQRRLDHCFSLFPRLSERKTQIAGTLSGGEQQMLAT
                     ARALMFEAKLLAIDEPSLGLAPRIREELFAAVGKIHAEGVPVLLVEQEVAQVFRMARR
                     NYVLSQGRIIAEGSAQSLMADETLRAGYLGL"
     gene            complement(6871..7947)
                     /locus_tag="VFR57_12185"
     CDS             complement(6871..7947)
                     /locus_tag="VFR57_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0260161.1"
                     /translation="MYWLKRGWAELHNEVLVLPSRTLVLLWVAALLLLPVVYSDAYVL
                     RIVTMTCIFAIFAASWDLLAGYTGQVNFGHALFFGAGAYTSALLSLKLGLSPWATVWA
                     GAAVALLFGFVVGYLCLRLRGSYLSLATLAFPLIALGVLFAFPDFSGGELGISGLRRL
                     AVTPTSNYYVAAISMLFIVFALWIFADSKFGIVLHAIRDDEVAARASGINTLRYKLIV
                     FAIAGAAAGFAGALFAHYLRVAGPSTLEVALSFQVVIWGIFGGVATVYGPVAAVFILY
                     PLTEWLGSFRAFGELRLLIFAIIVLLVLLFMPRGLTPWVRDKIETQCPRCKQRNSSWK
                     KICRLCGAGLEAQVPGAQRLVGHQ"
     gene            complement(7947..8816)
                     /locus_tag="VFR57_12190"
     CDS             complement(7947..8816)
                     /locus_tag="VFR57_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0260162.1"
                     /translation="MLQDIFVTGLVNGGVYALLAIGFSLIFGVARIVNIAHTAFYMLA
                     AYCFYTLLTKTGLGFVLSGVVAVAAVTLLSVVCYRLVIEPVREHEAAVLIATIALALI
                     FQEVMLYTFGGHFLGIPSTLEGAVRFLGVTIPYQRLLILVVAAAMLIIVWFVLYRTRL
                     GLAIRATANDLEVANLMGMNVHRVAMATVAISVALAAVAGVVVAPIFVVDPFMWLAPL
                     VTMLAIVVLGGLGSLKGSLIGALIIGYVEAITVFAVPAGAYLKGAVALMIMVAVLLAR
                     PEGLFGVAFEEER"
     gene            complement(8824..10041)
                     /locus_tag="VFR57_12195"
     CDS             complement(8824..10041)
                     /locus_tag="VFR57_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0260163.1"
                     /translation="MLRSFITGLAAFAIALGAQAQTIKIAILGPMAFVQGENHWAGAE
                     MARDEINKAGGINVGGKRMQVELIRADTNEIQSVPDATNAIERVITRDKADFLIGGFR
                     SEAVLAMQEVAMDHKKIFLGAGAAHSKLGQNVEENYNRYKYWFRYTPTKDIDLARTLF
                     AVMGSIAQQIRTELKTETPKVALLAEKAVWTGGLIAAAQKNLPAMKMEVVGLWQPSAT
                     ATDVTAELAAIDRAGAQIVFTMLSGPVGIVVGRQMGERNMKAVAWGINVEGQKEEFWQ
                     ATAGKAQFVSTLDTYAEVEMTPRTIPFVRAFKERYKKMPTYNAATYDAIIMLKSVIEK
                     ENTLDADKLVAAIEKVEHVGTGSIVTFDKRHDLVWAVGKTAGIAVQWQDGKKVPFWPP
                     QVKGMQPFKLPAR"
     gene            10102..10542
                     /locus_tag="VFR57_12200"
     CDS             10102..10542
                     /locus_tag="VFR57_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0260164.1"
                     /translation="MIWWEDFKLGEAAELGKHTFTEHEIVAFARQFDPQAFHIDPATA
                     QKTVFGGLIASGWHTCAVGMRMLCETLVNHTVSLGSPGIDHIRWLKPVRAGDTVTYRR
                     IVVESRASTTRKAVGLVKHRWEAVNQHGELVLTMEGWGFFGRKP"
     gene            10542..11279
                     /locus_tag="VFR57_12205"
     CDS             10542..11279
                     /locus_tag="VFR57_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU0260165.1"
                     /translation="MAAKIDLNHKVHVLTKKEELDTVRLPGKVVVVLDILFATTTMIT
                     ALAHGAKEVVPVLDESAAHAESKQLPERSFVLAGELYAETLPGFAHPAPLSLLAHGVE
                     GKSVIYSTTNGTVAMGLACSAARVYCGALLNARALVEHIVKHHPGETVLIVCSGSGNN
                     FNFEDFYGAGYFVECLQPHVTDLSDAAKAALALYRHAKAPEALLDCRVGRMMAARGLT
                     REVEFACRRDAYPVVAALDQGRVRLLV"
     gene            11279..11983
                     /locus_tag="VFR57_12210"
     CDS             11279..11983
                     /locus_tag="VFR57_12210"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU0260166.1"
                     /translation="MRVSQLFVYPVKGCRAVAVDQATLGPLGLEHDRRFAFVGVDGRA
                     VTQRDQPLLATIAATLDEGVLRLDFGGLTETAVPLGSFTAETSVDVWGTRLPAHGASA
                     DPASDYLGMRVSLVILDANARRSFADSKPVLVTTTGMLSELGVPGVGMDRFRPNIVLE
                     GNEPWRELRGGDAVLESVEPCSRCEVTTIDQESGARRGPEPLRTLNERLAGNFGVYCR
                     VTRGGRLRRGETLIAA"
     gene            complement(11980..12732)
                     /locus_tag="VFR57_12215"
     CDS             complement(11980..12732)
                     /locus_tag="VFR57_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0260167.1"
                     /translation="MKKNRLRVVTLNIHKGLSQFNRRLVIHELRDGLRALDPDLVFLQ
                     EVQGLNQRFALRFAGCPIDPQHAFLAGEDWEHAYGCNAVYDHGHHGNAILSRFGFVSF
                     ENEDVSAHRYERRGLLHSVVPVPGWRRNLHCVCVHLSLHERGRSRQLEAISGRLEELA
                     SRDLPIIVAGDFNDWRHRASSILERRLGMTEVLVAHYGRPVATYPSKLPLLKLDRIYV
                     RGFNVTAAQVHRGKPWSMLSDHLAVSADLERA"
     gene            complement(12729..>14411)
                     /locus_tag="VFR57_12220"
     CDS             complement(12729..>14411)
                     /locus_tag="VFR57_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260168.1"
                     /translation="SGRLTGWNVAYTFEIFPQWQHRFGPGVTHNTERLFSLQLPERTP
                     VSIAPKEHTAFAWLPWREAARKCFSWSNRDAILMVGAALLTAGCATGQAPLAPHLDAG
                     GTAEVRECAQWYRALDAEIDAAGVRDAQYTRVPGFPHLRVDRTLASLKDRAAQSESGL
                     RTFNERLAELDADSRVYEVRNVPTLNDDARAAALRRARDCGKRLREADLASPGARESL
                     LAAASVPDDYSTASRFFGLYYLTRIPFASGVRNWQNDTVAAFQRSGDPAANRVRYAPP
                     QGQALPRSVVAGLLGRAAFDPLGHPAVSDRELERLALTYAPTFEVEIMGDYDRFGALR
                     WQRAATSLEVDATEPSVYVQAAYMRYHDRLLLQLVYTLWFPERPRTGSFDLYGGRLDG
                     VVWRVTLAPDGEPILYDSIHPCGCYHIFFPTPRARARPAPDDLEEWAFIPQSVPRVGE
                     GERAVLTIASATHYIERIGFEPGAASLVRYRFRRYDELRSLPHPGGAHASAFGPQGLI
                     AGTERLERFFFWPMGIASAGAMRQWGRHATAFVGRRHFDDADLLERRFELDL"
BASE COUNT         2531 a         4735 c         4618 g         2527 t
ORIGIN      
        1 gtagttcttc gcctcgcccc cgtacttctt cgccagtatg tgcgtcatcg ccgccgtcag
       61 cgtggtcttg ccatgatcca cgtgtccaat cgtgcccacg ttcacgtggg gcttcgtacg
      121 ctcaaatttg cttttggcca tgttatgcgc tcctacttct tttcctcgtc ttttttcatg
      181 attgcgtcgg cgacgctctt cggggcttcg gcgtagtgct tgaactccat cgtgtacgtc
      241 gcgcggccct gcgtcagcga gcgcagcgtc gtggagtagc caaacatctc gccgagcggc
      301 acctcggccc ggatcgcctt gctgccgggc atgtcctcca tgccctggat cacgccgcgg
      361 cggcccgaga ggtcgccgat cacgtcgccc atcttctctt ccggcgtctc cacctcgacc
      421 gccatgatcg gctcgagcag aacgggctgc gccgagcgca tgccgtcctt gaaggcgaag
      481 attgcggcca tcttgaaagc gttctcgttc gagtcgacct cgtggtacga gccgtcgaac
      541 agcgacactt tgacgtcgac gaccggatag cccgcgagca cgccgtcctg catcgcttcg
      601 ttcacgcctt tctcgaccgc agggatgtac tccttcggca cgcggccgcc cttgatggcg
      661 tcgacgaact cgtagccctt gccctgcacc tgcggctcga gcttgagcca aacgtggccg
      721 tattggccgc ggccgcccga ctgtttgacg aacttgccct cggactcgat cgcttttcgg
      781 aaggtctcgc ggtaggcgac ctgcggtttg ccgaccgaag cctcgacgcc gaactcgcgc
      841 ttcatgcggt cgacgatgat ctcgagatgc agctcgccca tgcccgagat gatcgtctgc
      901 cccgattcct cgtcggtgcg aacccggaac gaggggtctt cctgagccag ccggttcagg
      961 gccatcccca tcttttcctg atcgaccttg gtcttcggct caacggcctg cgcgatcacc
     1021 ggctcgggga actccatacg ctcgagggtg atcacgttct tcacgtccga gatcgtctcc
     1081 ccggtcacga cctccttcag cccgaccacc gcggcgatgt cgcccgcacg cacttccttg
     1141 atctcctcgc gctcgttggc gtgcatctgc agcagccgcc cgatgcgttc cttcttgccc
     1201 ttcatcgagg tatagacggt atcgccggaa gtgagcacgc ccgaatacac gcggatgaac
     1261 gtgagctgac cgacgaacgg gtcggtcatg atcttgaacg cgagcgcggc gaacggctcg
     1321 tcgtcgccgg gcttgcggaa ttcgggctcg cctttctggt tctccccctt gaccggcggc
     1381 acatcaatcg gcgagggcag gtaatcgatg acggcgtcaa gcattgcctg cacgcccttg
     1441 ttcttgaacg ccgagccgca cagcatcggc acgatctcgt tgttgatcgt gcggatgcgg
     1501 atgcctttct tgatttcctc gaccgagagc tcgcccgact cgaggtactt gttcatcagc
     1561 tcttcgttcg cctcggccgc agcctcgacc atcttgtcgc gccactcctt ggcttcggcg
     1621 gcgagctcgg ccgggatggg cctcctctcg aatttcatgc cctgcgtggc gtcgtcccag
     1681 tagatcgcct gcatgttgac gaggtcgatt accccggcga acttttcttc ggcgccgatc
     1741 ggaagctgga ttgggaccgg gttgcccttc aggcgagtgc gaatgtgctc gtaggacttg
     1801 aagaaggtcg cgccgacccg gtccatcttg ttgatgaagg cgagccgcgg cacgcggtac
     1861 ttgttggcct ggcgccacac cgtctcggac tgcggctgca ccccggcgac cgcgtcgtac
     1921 accatgcacg cgccgtcgag cacgcgtagc gagcgctcca cctcgatcgt gaaatcgacg
     1981 tgaccaggcg tgtcgataat gttgatccgg tgctcgggat aggaaagatc cattcccttc
     2041 cagaagcacg tggtcgcggc cgacgtgatc gtgatgccgc gctcctgctc ctgctccatc
     2101 cagtccatgg tggcagcgcc gtcgtgcacc tcgccgagct tgtggttgac gcccgtgtag
     2161 aacaggatgc gctcggtcgt ggtcgtcttc ccggcgtcga tgtgcgccga gatgccgatg
     2221 ttccgatacc gttcgattgg agtcttgcgt gccacggcca ttcaaaacct gtagtgcgag
     2281 aaagccttgt tcgcttcagc catacgatgg acctcctcgc gcttcttcat cgcaccgccg
     2341 cggccctcgg ccgcttcctg cagctcgccg gcaaggcgaa ccgtcattga cttctcgccc
     2401 cgcttctgcg ccgcctcgcg gatccagcgc atcgcgagcg ccatgcgccg caccgggcgt
     2461 acctcgaccg gcacctggta gttcgcgccg cccacccggc ggctcttcac ttcgacgacc
     2521 ggctttacgt tctgcaccgc ctgcgtgaaa acctcgatcg gatcctttcc cgacttcgac
     2581 ttgatggtct ccagcgcgcc gtagatgatg cgctcggcca ccgacttctt gccgcgcgtc
     2641 atcagcacgt tgacgaactt ggcaacttcg acgttattga acttcggatc cggcaggatc
     2701 tcacgcttgg ggacttctct gcgccgcggc atctgaaatc cctaggccga cttcggcgcc
     2761 tttgcgccgt acttggagcg gccctgcttg cggtccttca ctccctgagt gtcgagcgag
     2821 ccgcgcacga tgtggtagcg cacgcccggc aggtccttga cgcgcccgcc gcggatcagc
     2881 accaccgagt gctcctggag gttgtggccc tcgccgccga tgtagccgat cacctcgaag
     2941 ccgttcgtga ggcggacctt cgcaaccttt cgcagcgcgg agttcggctt ctttggcgtg
     3001 gtcgtataga cccgggtgca gactccccgc ttctgcggcg agccggcgag cgccgggact
     3061 ttcgacttgg cgcgcttggc ggcgcgccca tcacgcacta actggttgac cgtgggcatg
     3121 tttttgcgtt tccagaaacg aaacaggcgg ccccgattgc tgctccccgt aggccgcccg
     3181 gacccaaaaa agaggccgga ttataaagag gaaactaccc cagcgcaacg actggggacg
     3241 ggtcagattc cggggtccga aaagaaattg aagcgggttt cgacttccga gcgccggatc
     3301 ctcggcaacg cctgcgccga atccgcataa cgggcgaggt cgcgatcgtc gagcaaagcg
     3361 attccacgct ccccctcgag cagcaccgag ccttcgtcgt cctgaaacgc gttcctgcag
     3421 acaaacggcc ggccgcaatg gtcgtaaagc cgatcgccct cgtagtgcac cacgagcggc
     3481 gtgtaggcaa gcgtgacgta cacccgttgc ggcccgttct ggaagaacca gcgcccgcgc
     3541 tcgtcggcct cgtagttccg cccgatgaac tcgcgtagag cagcgttgcc gatgcgctcg
     3601 cctttgatca gccagttgcc gcgccgatcg agctcgagcc agccgtatac cgcggggaca
     3661 ttgggccact tggccaggct gcgggcgacg atctcatcca tcgagggata gaatcttcgg
     3721 cgaggaggag ggaggcgtct agcgatgtcc gcaatcgaaa cttaccttgc gaagccctgg
     3781 ctggcgcact accagaaggg agtgcccccc acggtcgaag ttccgctgaa atcggtggcg
     3841 caggcgttcg acgaggcgac tgagcgcgcc ccggagcgcc cggccttagt gttctacggc
     3901 cgctcgatcg cctaccgcga gctgcgcgag gcaaccgacc gcctggcgtg cgcgcttgcc
     3961 gatctcggcg taagaaaagg cgaccgggtc gcgctgtatc tgctcaacag cccgcagttc
     4021 atcattgcgt acttcgcggc actcaagtgc ggcgcgaccg tcacgccgat cagcccggtc
     4081 tacaccagcc acgaggttgg ctatcagctc aaggacagcg gcgcacgcgc catcatctgc
     4141 caagacatcc tgcacgaaaa ggtggtcaag tgcggcgcgg cgctcgacct ggtcgtggtt
     4201 accaatgtca acgagtactt gccggccctc aagcgcctct tcggcaagaa ggccgaggtc
     4261 accggaaggg tgcactggct gcaggatttg ctcaagaaat accccgccaa gccgcctgcg
     4321 gtgacgattg atccgaagaa agacctggct gccctgccgt ataccggagg cactaccggc
     4381 agcccgaagg gcgtgatgct cacgcactac aacctggtcg ccgcgcaagc catgggccaa
     4441 agcgctttcc cggtcttcga gcaggggaaa gaagtaatcc tcgccttcct gccgttcttc
     4501 cacatctacg gacaggtggt gatcatgctt caggggctgt gccagggaca cctgctggtg
     4561 cttttcacca gccccgatac cgaagccatt ctcgacgcga tggagcgcta cggcgccacc
     4621 gtgttctacg gcgtgcccac gctgtacgaa tacttgaagg accacaagga caccaataag
     4681 gcgaactggc ggcgcctgaa gctcatcgtc tccggcgcgg acacgctgca cgaatcgacg
     4741 acccaagggt ggacgcggcg cacggggtcc accatcaccg agggctacgg gctttcggag
     4801 acctgcgcca ccagccacat caatccgctg cagcggccca aggccggatc tttcggctgc
     4861 ccgatcacaa gcatgcaggc ggcggtgatc gacccggagt ccctggagtt cgtgccggcg
     4921 aacgagacgg gcgagctggt gctctgcggc ccgaacgtga tgcaggggta ctggcagcgc
     4981 gccgaggaca ccgcgcgcgc ctttgtcgac aagggcggcg tacggtggat gcgcaccggc
     5041 gacctggtgc gcatggacga cgaaggctac ttccacttct acgaccgctc caaggacctc
     5101 atcaagtaca agggttactc gatcttcgcc aaggacgtcg aggacgtcct ctacgcgcac
     5161 ccgcaggtca aggcggccgg cgtgatcggc gtacccgatc cggccgtcgg acagcgcatc
     5221 aaggcaatcg tggtcctgca gggcgacgcg cgcggcaagc tttcggagga cgagatcaag
     5281 gcgtattgcc ggcaaagcct cgccgagtac aaggtgccgc acatcgtgga gttccgcggc
     5341 gagctgccca agaccgacgt cggcaaagtg tcgcgccgcg agttgcgcga cgagaccaag
     5401 gtctagtctt ggccgggcct tttttcgcgg tcgagacgct gaccaagcgt ttcggtgggc
     5461 tcaccgcggt caacaacgta agcttctcgc tgcggcgcga tgaaatcgtc ggcctgatcg
     5521 gcccgaacgg cgccggcaag acaacactgc tgcgcctcat caccaagatc ctgaagcccg
     5581 atgccgggcg aatcgtgttc aacggcgaga acatcacggc cctgaggccc tgggacgtcg
     5641 tcgagcgcgg cattgcgggc accttccaaa acacgcggcc cttccgccat ctgccgatcg
     5701 tcgccaacgt catggtgccg ctggtggccc cgcgcgcgcg ccgtggcggc gaatgggtaa
     5761 agcgcatcga ggcgaaagcg atggacgcgc tcgagttcgt cggcatctcc gacatggcgc
     5821 tcgaacccgc ctccgcgctg tcgcagggcg acttgaagcg cctggaagtg gcgcgcgcca
     5881 tcgccaccga gccggagctg ctgctgctcg acgagccgct cggcggcctc aacccggccg
     5941 agtccgagct gcttgcgaac tcgattcgcc gcctgcacaa gggcggacgc ttcggcagac
     6001 tgcattccga gggcccggcg gtgctgatga tcgagcacaa gctgaaggag ctgatgtcga
     6061 tcgtcgaccg ggtaatcgtg atcgactacg gcgagatcat cgccgacgcg ccgccggccg
     6121 aggtggtgaa aaatccgaaa gtgatcgaag cctacctcgg gagcgagcat gccgctgctt
     6181 gaggtgagga acctgcgcgt gcgctatgac aaagcgcaga tcctgaacgg cgtgacgctc
     6241 tcggtgaacg aaggcgagct ggtcggcctg gtggggccga acggcgccgg caagtcgacg
     6301 ctgctgcgcg cgatctcggg cctggtgcac ttcgaggagc gcatgaagcg cggcacgcac
     6361 ggcgacatcg tgctcgaggg cgaagtgcgc ttcgccggcg agcgcatcga cggcctcgcc
     6421 gcacacgaga ttcgcatgcg cggcctggtg cactgcccgg agcgccggcg cccgttccgc
     6481 gagcttacgg tccaggagaa cctgctcgcc ggcgcctttc tttcgacaag caaggaacag
     6541 acgcaacgtc gtctcgatca ctgcttttcg ctcttccctc gtctgtcgga aaggaagacg
     6601 cagattgcgg gaacgttgtc ggggggcgaa cagcagatgc tcgccacggc gcgggcgctc
     6661 atgttcgagg cgaagctgct cgcgatcgac gagccctcgc tcgggctcgc gccgcgcatc
     6721 cgcgaggagc tgtttgccgc ggtcggcaag atccacgccg agggcgtgcc ggtccttctc
     6781 gtcgagcaag aagtcgcgca ggtgttccgg atggcaagac ggaactacgt gctgtcccag
     6841 ggccgtatca ttgccgaggg ctcggcgcag tcactgatgg ccgacgagac gctgcgcgcc
     6901 gggtacctgg gcctctagcc cggcgccaca caatcggcaa atcttcttcc acgaagaatt
     6961 tctttgtttg cagcgcgggc attgcgtctc gatcttgtcg cgcacccacg gcgtgagccc
     7021 gcgcggcatg aaaagcagca ccagcaggac gatgatggca aagatcagca ggcgcagctc
     7081 gccgaaggcc cggaagctgc cgagccactc ggtcagcgga tagaggatga acaccgccgc
     7141 gaccgggccg tagacggtcg cgacgccgcc gaagataccc cagatcacca cctggaagga
     7201 aagcgcgacc tcgagcgttg aaggaccggc gacgcgcagg taatgcgcaa acagtgcgcc
     7261 ggcgaacccc gccgccgcgc ctgcgatcgc gaacacgatc agcttgtagc gcagggtgtt
     7321 gataccggag gcgcgcgcgg cgacctcgtc gtcgcggatg gcgtgcaaga cgattccgaa
     7381 cttggagtcg gcgaagatcc acagcgcaaa gacgatgaag agcatcgaga ttgccgccac
     7441 gtagtagttg ctggtcggcg ttacggcaag gcggcgcaat ccggagatgc cgagctcgcc
     7501 cccggagaag tccgggaagg cgaacagcac gccgagcgcg atcagcggga acgcgagcgt
     7561 ggcgagcgaa agatacgagc cgcgcagccg caggcacaga tagccgacga cgaagccgaa
     7621 cagaagcgca accgccgccc ccgcccagac cgtcgcccac ggcgagagcc cgagcttgag
     7681 ggagagcagc gccgaggtgt aggcgccggc accgaagaac agcgcgtggc cgaagttcac
     7741 ctgcccggta tagccggcga gcaggtccca gctcgcggcg aagatcgcga agatgcacgt
     7801 catcgtgacg atgcgcagaa cgtaggcgtc gctatacacc accggcagca ggagcagcgc
     7861 cgcaacccac agcagcacga gggtgcgcga cggcagcacg aggacttcgt tgtgcagctc
     7921 ggcccacccg cgcttaagcc aatacatcag cgttcctcct cgaacgccac cccgaacagg
     7981 ccctcgggcc tggcgagaag cacggcgacc atgatcatca gcgcgaccgc gcccttgagg
     8041 tacgccccgg ccggcaccgc gaaaaccgtg atcgcctcga cgtagccgat gatcagcgcg
     8101 ccgatcaggc ttcccttcag gctgcccagc ccgccgagca cgacgatcgc gagcatggtg
     8161 accaggggcg cgagccacat gaacggatcc accacgaata taggcgcgac caccaccccg
     8221 gcgaccgccg cgagtgcgac cgaaatcgcc accgtggcca tcgccacccg gtgcacgttc
     8281 attcccatga ggttcgccac ttcaagatcg ttcgcagtcg cacggatcgc gagccctagg
     8341 cgtgtgcggt agagcacgaa ccagacgatg atcagcatcg ccgccgccac cacaaggatc
     8401 agcaagcgct ggtacgggat ggtgacgccg aggaagcgca ccgctccctc gagcgtggac
     8461 gggatgccga ggaaatgccc accgaaggtg taaagcatca cctcctggaa gatgagggcg
     8521 agcgcaatgg tcgcgatcag taccgcggcc tcgtgttctc gcaccggctc gatcaccagg
     8581 cggtagcaca cgaccgatag gagcgtgacc gcggcgacgg ccaccacgcc ggagaggacg
     8641 aagccgaggc ctgtcttcgt gagcaacgtg tagaagcagt acgccgccag catatagaag
     8701 gcggtgtgcg ctatgttgac gatgcgcgcc accccgaaga tgagcgaaaa gccgatcgcg
     8761 aggagcgcgt aaacgccccc gttgaccagg ccggttacga agatgtcttg tagcaaactg
     8821 gcgctagcgc gccggcagct tgaacggctg catgcccttc acctgcggcg gccagaacgg
     8881 cactttcttg ccgtcctgcc actgcacagc gatgcccgcg gtcttgccca ccgcccagac
     8941 gagatcatgg cgcttatcga aggtcacgat gctgccggtg ccgacgtgct cgaccttctc
     9001 gatcgccgcg accagcttgt cggcgtcgag cgtgttttct ttttcgatca ccgacttcaa
     9061 catgatgatg gcgtcgtagg tcgcggcatt gtaggtcggc atcttcttgt agcgctcctt
     9121 gaaagcgcgc acgaagggaa tggtcctggg cgtcatctcg acctcggcat acgtatcgag
     9181 cgtcgagacg aattgcgcct tgccggccgt ggcctgccag aactcctcct tttggccctc
     9241 gacgttaata ccccaggcca ctgccttcat gttgcgctcg cccatctgcc ggccaaccac
     9301 gatgcccacc gggccggaga gcattgtgaa tacgatctgc gccccagcgc gatcgatggc
     9361 cgctagctcg gcggtaacgt cagtcgcagt tgccgagggt tgccacaggc ctacgacttc
     9421 catcttcatc gccggcaggt tcttctgcgc cgccgcgatc aatccgccgg tccacaccgc
     9481 tttctcggcg agcagcgcga ccttcggcgt ctcggtcttg agctcggtgc gaatctgctg
     9541 cgcgatggag cccatcaccg cgaacagcgt gcgcgcgagg tcgatgtcct tggtcggcgt
     9601 gtagcggaac cagtacttgt agcggttata gttctcctcg acgttctgcc cgagcttcga
     9661 gtgcgcggcc cccgcgccga ggaagatctt cttgtggtcc attgccactt cctgcatcgc
     9721 gagcaccgcc tcggatcgga acccgccaat caggaagtcg gctttgtctc gcgtaatcac
     9781 acgttcgatc gcgttggtgg catcggggac cgactggatc tcgttggtgt cggcgcggat
     9841 cagctccacc tgcatgcgct tgccgccgac gttgatgccg cccgccttgt tgatctcgtc
     9901 gcgcgccatt tcagcgcccg cccagtggtt ctcgccctgc acgaaagcca tcggcccgag
     9961 gatcgcgatc tttatggttt gcgcctgagc accgagcgcg atcgcgaagg ccgcaaggcc
    10021 cgtaatgaac gaacgcagca tgtcccgcct cctttttgaa ctacacttta tcttgttgta
    10081 ctcgaagatg ttgccagcaa tttgatctgg tgggaagact tcaagctggg cgaggctgct
    10141 gagctcggca agcacacgtt cacggagcac gaaatcgtgg cctttgcccg ccaattcgac
    10201 ccccaggcat tccacattga cccagccacc gctcaaaaga cggtgtttgg cggcttgatc
    10261 gcctcgggct ggcatacctg cgcggtcggc atgcggatgc tttgcgagac actcgtcaac
    10321 cataccgtga gcctcggctc gccggggatc gaccacatcc gttggcttaa gcccgtgcgc
    10381 gcgggcgata cggtgaccta ccgccgcatc gttgtggaat cgcgcgcctc gactacgcgt
    10441 aaggccgtcg gtctagtcaa gcacaggtgg gaggcggtca accagcacgg cgagctcgta
    10501 ctgaccatgg aaggctgggg gttcttcggc cgcaagccct gatggcggca aagatcgacc
    10561 tcaaccacaa ggtccacgtc cttaccaaga aggaggaact ggacacggtg cgcctgcccg
    10621 gcaaggtggt ggtggtcctc gacatcctgt ttgccaccac gaccatgatt acggcgctcg
    10681 cgcacggcgc gaaggaggtc gtgccggtac tggacgagtc ggcggcgcac gccgaatcga
    10741 agcaacttcc ggagcgttcg ttcgtgctcg ctggcgagct ttacgccgag acgctaccgg
    10801 gcttcgctca tccggccccg ctttcgctgc tcgcgcacgg cgtcgaaggc aaaagcgtca
    10861 tctattccac caccaacggc accgtggcaa tggggctggc atgctccgcc gcgcgcgtct
    10921 attgcggcgc gctgctcaac gcgcgcgcgc tcgtcgagca catcgtgaag catcatcccg
    10981 gcgaaaccgt gcttattgtc tgctcgggct cgggcaacaa cttcaacttc gaggatttct
    11041 acggcgccgg atacttcgtg gaatgcctac agccgcacgt cacggacctt tccgacgccg
    11101 caaaggctgc gctcgcgctc taccgccatg cgaaggctcc tgaagcgctg ctcgattgcc
    11161 gcgtcggccg catgatggcg gcgcgcggct tgacgcgcga agtcgaattc gcctgccggc
    11221 gcgacgcgta tcccgtggtt gccgcgctcg atcagggccg cgtgcggctg ctcgtgtagt
    11281 gcgcgtctcg caactgttcg tctatccggt gaaaggctgc cgcgccgtgg cagtggatca
    11341 agccacgctc ggcccgctcg gcctcgagca cgatcgccgc ttcgcgttcg ttggcgtgga
    11401 tggccgggcg gttacgcaac gcgaccagcc gctgcttgcg acgattgccg ccactctcga
    11461 tgaaggtgtg ttgcgcctgg acttcggggg tcttacggaa acggcagttc ctctgggtag
    11521 ttttacagca gaaacgtcgg ttgatgtttg ggggacccgc ctacccgcgc atggggcgtc
    11581 tgcggatccg gcaagcgatt atctcggcat gcgggtatcg ctggtcatcc tggacgccaa
    11641 tgcgcggcga agcttcgcgg actccaagcc ggtgctggtt accacgacgg gcatgctgtc
    11701 cgagctcggc gttcccggag tcggcatgga tcgcttccgg cccaatatcg tgctcgaggg
    11761 aaacgagccg tggcgcgagc tgcgcggggg cgacgccgtg ctggaatccg tcgagccctg
    11821 cagccgctgt gaagtgacga caatcgacca ggagagcggc gcacgccgcg gcccggagcc
    11881 gttgcgcacc ctgaacgagc gcttagcggg caacttcggc gtctactgcc gcgtcacgcg
    11941 cggcggccgc ctgcgccggg gcgagactct gatcgccgcc taggcgcgct cgaggtcggc
    12001 gctcaccgcg agatggtccg acagcatgga ccacggcttc ccgcggtgca cctgtgcggc
    12061 cgtgacgttg aatccgcgca cgtagatacg gtctagcttg agcagcggca gcttgcttgg
    12121 gtaggtcgcg accggacggc cgtagtgtgc gaccaaaacc tccgtcattc ccaggcgccg
    12181 ctctaggatg ctgctcgcgc ggtggcgcca gtcgttgaag tcgccggcga cgatgatcgg
    12241 cagatcgcgc gaagcgagct cttccagccg ccccgagatc gcctcgagct gccggctgcg
    12301 gccgcgctcg tgcagcgaaa gatgcacgca cacgcaatgc aggttgcgcc tccatcccgg
    12361 caccgggacc acgctgtgca ggagtccgcg gcgctcgtag cggtgggcgg acacgtcttc
    12421 gttctcgaat gacacgaagc cgaaacgcga caggatcgcg ttgccgtggt gaccgtggtc
    12481 gtagaccgcg ttgcagccgt aggcgtgctc ccagtcctcg cccgcgagga atgcgtgctg
    12541 cggatcgatc gggcagccgg cgaagcgcag cgcgaagcgc tggttcagcc cctgcacttc
    12601 ctgcaggaac acgagatcgg ggtcgagcgc gcgcaggccg tcgcgcagct catggatcac
    12661 caggcgccgg ttgaactgcg acaacccctt gtggatattg agcgtcacca cgcgcaggcg
    12721 attcttcttc ataagtcgag ctcgaagcgt cgctcgagca ggtcggcgtc gtcgaagtgg
    12781 cggcggccga cgaacgcggt ggcgtgccgg ccccactggc gcattgcgcc ggcggaggcg
    12841 atgcccatcg gccagaagaa gaagcgctcg aggcgttcgg tgccggcgat cagtccttgc
    12901 ggaccaaagg cgctggcgtg cgcgccgccc gggtgcggca gcgagcgcag ctcgtcgtag
    12961 cggcgaaagc ggtagcgcac gaggctcgcg gcgcccggtt cgaagccgat acgttcgatg
    13021 tagtgagtgg ccgatgcgat ggtcaatacc gcgcgctcgc cttcacccac cctcggcacc
    13081 gattgcggga tgaacgccca ttcctccaga tcgtccggcg cggggcgcgc gcgggcgcgc
    13141 ggagtcggga agaaaatgtg atagcagccg caggggtgga tcgagtcgta aaggatcggc
    13201 tcgccatccg gggcgagcgt cacgcgccat accactccat ccaggcgacc cccgtacagg
    13261 tcgaacgacc cggtgcgcgg ccgctcgggg aaccagagcg tatacacgag ttgcagcagc
    13321 aggcggtcgt ggtagcgcat ataggcggcc tggacgtaca cggatggctc ggtcgcatcg
    13381 acttcgagcg aggttgcagc gcgctgccag cgcagcgcgc cgaagcggtc gtagtcgccc
    13441 atgatctcca cctcgaaggt cggcgcgtaa gtcagcgcca ggcgttccag ctcgcggtcg
    13501 gaaaccgccg gatggccgag cggatcgaag gccgcgcgcc caaggagccc ggcgacaacg
    13561 cttcgcggca aagcctgccc ctgcggcggg gcgtagcgca cgcggttcgc cgccgggtcg
    13621 ccgctgcgct ggaatgccgc aacggtgtcg ttctgccaat tgcgtacacc ggacgcgaac
    13681 gggatcctcg tcaggtaata caggccgaaa aagcggctcg cggtggagta gtcgtccggc
    13741 acgctggcag cggcgagcag cgattcgcgc gcgccgggac tcgccagatc cgcttcgcgc
    13801 agccgcttgc cgcaatcgcg ggcccggcgc agggccgcgg cgcgggcatc gtcgttcagc
    13861 gtcggcacat tccggacctc gtacacgcgc gagtcggcgt cgagctcggc gagccgctcg
    13921 ttgaacgtgc gcaggccgga ctcgctctgc gccgcgcggt ccttcagcga agcgagcgtg
    13981 cggtcgacgc gcaggtgcgg aaacccgggc acgcgcgtgt actgggcgtc gcgcactccg
    14041 gcggcgtcga tctctgcatc gagcgcccgg taccactgcg cgcactcgcg cacctcggcg
    14101 gtccccccgg cgtccaggtg cggagcaagc ggcgcttgtc cggtcgcgca tcctgccgtg
    14161 aggagcgcgg cgcccaccat cagtatcgcg tcgcggttgg accacgagaa gcacttgcgc
    14221 gcggcctcgc gccacggcag ccaggcgaaa gccgtgtgct ctttcggcgc aatagacacg
    14281 ggcgttcgct ctggaagctg caaactgaag agccgctcgg tgttgtgggt gacgccgggg
    14341 ccgaagcgat gctgccattg agggaagatt tcgaaggtgt aggcgacgtt ccagccggtg
    14401 aggcgtccgc t
//
LOCUS       DASTPD010000361        11832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1425457, whole genome shotgun sequence.
ACCESSION   DASTPD010000361 DASTPD010000000
VERSION     DASTPD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425457"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFR57_12225"
     CDS             <1..1007
                     /locus_tag="VFR57_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0260169.1"
                     /translation="RAQFIEEWRKNRRSVIRKEDVALQPTSRGMRTGVYMGWDGDSPT
                     RCLDALVHELDPGADTTIHRHSWDAQLFIVEGSGWSEIDGVRYRWKPWDAIHIPAWSW
                     HRHGNDGSRRARFMSYSSEPMLWTLGLCVLEDRGYEDVRDLPPPPASSPGIDGDDPYS
                     RRRRRIFQETKKRRGGRIHTPYDEQDLLATPRGTRTKFLVDRAIGSESSGITQVMIQF
                     APGKTQSLHRHPGEAWLYVVEGYGHSFMGTAPDRGQHYRWKKGDLIVVDHFLWHQHFN
                     DDPKGEARLVRVHMFDSILETMRALTDPLVLFEEAEDTLLSMQEISQIEWPDDRRPEG
                     "
     gene            1021..1992
                     /locus_tag="VFR57_12230"
     CDS             1021..1992
                     /locus_tag="VFR57_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0260170.1"
                     /translation="MAAPAPALPPGIVSLHTLPAEHHDKDWDAIIVPAGVKERLLAQA
                     LLTLKHGHKLATLAGLPHGLIVLAGPPGTGKTTLARGLAQVSALALAREGATTLLEIN
                     PHAFPSDMLGESQRNIMRLLTDTIPEIAARRPHTVVLIDEVESFAVRRSTASFETNPV
                     DVHRATDAVMLGIDEVARKLPAVLFVTTTNFIDAIDEAFLSRADLVMHFALPDPGTIA
                     RILHDSLAELAVQWPALKPLLQSEDEINEVARLCGGWSGRQVRKLVLAALAQRLEVAK
                     DPGRLTFEDLREAARKRGGVEWVGAAGPSSKELFPDVPKHLMHDHHH"
     gene            2003..2992
                     /locus_tag="VFR57_12235"
     CDS             2003..2992
                     /locus_tag="VFR57_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0260171.1"
                     /translation="MRLVLGLIAALAAAPAPAQYYSGKTVTIVVGYKTGGGYDATARV
                     LARHLPKHIPGKPSVIVQNMPGANSIIAANHVYAVTKPDGLTIGTFNRNLPIAQLTKV
                     EGVKFDMAKFAWVGSAASECTVLAIRADLPYKTFEDLRKSKQQIVIGATGPGASTYDF
                     PLLLKEFLGVNVKLVSGYQSSADIMLAVERKEVDGRAGSFTSIRPFIDRNLVRPVLRA
                     RATEPGVENLPIDEDLAPTPRAKAIMALRSAPEIVGRPYVMHPNTPPEHLKTMRAAFA
                     AAIKDPELVAEARKAKMDLEFVDGDVAVKVMNEVLSQPKDIVDEFSKYIKFGE"
     gene            3002..4501
                     /locus_tag="VFR57_12240"
     CDS             3002..4501
                     /locus_tag="VFR57_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0260172.1"
                     /translation="MIEALGEGLFLVLQWKPLAFMMLGIVLGFWVGLLPGLGGATTLA
                     LMLPFIYAMQPVEAFAFLLGMHAVVATTGDITSVLFGVPGEATSAATILDGHAMAKKG
                     EAGRALGAALASSLVGAVVGAFALAIAIPMVRPLVLTFGSPELFMLAIVGIAFIASLS
                     APGVRGMVRGFLAGGLGLLCATAGQDPQAGIARYTFDSLYLWGGLDLVPVLVGVFAIP
                     EIIDLAIRRTSIAGELPAGRISRGAFEGVKDTFRHFWLTIRCSLIGTFIGIMPGMGGA
                     VAQWMAYGHATQSARNAEERKGFGAGDVRGVLGPGAANNSKEGGSLIPTVAFGVPGST
                     AMAILLGGFFLLGLVPGPDMLTKHLPVTFSMVWTIVIANLVTVIACFVFLDHLARVTT
                     VRGTVLIPVILVLIFIGSYTSNNHYGDILLTLVFGGVGYLMVVAGWPRAPFVLGLVLG
                     KIAENYLYISVARYDAAWLTRPVVLALFAIALAVILWPLLQRRRRAAHA"
     gene            4494..4964
                     /locus_tag="VFR57_12245"
     CDS             4494..4964
                     /locus_tag="VFR57_12245"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0260173.1"
                     /translation="MHRKASLALALGVMAVSGYAVIAAWSWPWKAALFPLVIGIPLFV
                     LSATEAAWVLFGKTGRAEVQDFQLARDVPEREVVRRSAVAAAWIVGFFAAIVLFGFAL
                     AVPLFVFLYLKLHANEGWIFSAVFTAAVWAFFYGLFDRLLHLPFPAGWALTWIA"
     gene            complement(4948..6441)
                     /locus_tag="VFR57_12250"
     CDS             complement(4948..6441)
                     /locus_tag="VFR57_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0260174.1"
                     /translation="MTWEFIAGQFAAQFGLWWIIWAGILLGIVVGILPGFSPQNTLIM
                     LLPLTLGIPVEQAFAFMVSLYCANHLAGGVPAILVKIPGSGGAAATTLDGYPMTQKGQ
                     AQQAIVLSFTASVFGGLITTLATLALLPWLSQIGLYLRSVEMVVVMLFGLTLIAVIAA
                     KDTLKGLISGLFGLLIGVIGTDHIYNAPRATFGFLELYDGVPLVAALIGLFAVSEALV
                     MIEEKLIVGREGRERAMRASWADTLEGLKLSVQKWWHIVWTSFVGLFIGIVPGAGASI
                     AAFVAYQQSRMWSKHPERYGTGIPEGVIAPEAANNGVTSGTLVPMMALGVPGGTTAAV
                     MMIVLQYHGMVMGPRLFTTNPELAYGVFVAMAVAYIFMIFTILPLARYMARIVLIETT
                     YLVPVIVLVTVVAAFVERQYLFDMGLALVFGVIGYIAHKTNYHVTAMLIGIILGPLFE
                     QYLARALRISQGDLTVLFSSTLGNILWAFLVLSLVLPVLKSRLRQSR"
     gene            complement(6448..6942)
                     /locus_tag="VFR57_12255"
     CDS             complement(6448..6942)
                     /locus_tag="VFR57_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0260175.1"
                     /translation="MIVPQKAPLGADLVIPVFALGFAVYFFWSIADLVWEAKANGVVI
                     GAVLVGLVGLQLGRIAVEVANGRGDLRTDPLWQPREVLFKRVGMVLVTIAFIALLQVL
                     GLTLSLFAAMAGALAIMGVRRLPILLALSLGVAAAAYLLFIAALDSAFPHGPIEQLLA
                     RLFA"
     gene            complement(6939..7952)
                     /locus_tag="VFR57_12260"
     CDS             complement(6939..7952)
                     /locus_tag="VFR57_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0260176.1"
                     /translation="MHPNPTRRRIVAGAGAALALGAVNVRAQAYPSKNMRVVIPTGQG
                     GGAERLARAFDAFWGPMLKTNFEYSFFPGAAGQVGYEVFVNKREKDGYNLLFGNMGPE
                     MIMYALQKPAYRFPEDYQYFCRTDIDDSVVFALRKSKFQRVEDLVAEAKRRVVNVAVS
                     RLPHPASIGMLALGAAVGAKFNLVPYGGGNPTTVAVLNGEADCGALPIAGVVAQREQM
                     KVLGVFNDENALAKYSDNAPPVNKVFGTKLPDLSSSRSWAIHTEVIEKFPDRFALLEK
                     TSRQVFDNPKYREEYAKTGAPVETIQYGDRALCTRYAKAMVELANEYRSLLTAKEKGK
                     GKG"
     gene            complement(8045..8701)
                     /locus_tag="VFR57_12265"
     CDS             complement(8045..8701)
                     /locus_tag="VFR57_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0260177.1"
                     /translation="MKRLLLIGAACLAAGLAGCGDPRSHAQAVYVLIDTSGTYAQEAA
                     KAQLIINYLLGTLQPGDSLAVARVESRSFSEKDIIAKATFDQRPSQANAQKRAFREAT
                     NNFLRNVKSSRYTDISGGLLQGAEFLNETGAGTKTLVVFSDMQEELDKVTVRNIPMNL
                     KRIRVIAVNVVKLNTDNIDPRRYFGRLEAWERRVVAAGASEWRLINDLERLDTIFSRS
                     "
     gene            complement(8701..10146)
                     /locus_tag="VFR57_12270"
     CDS             complement(8701..10146)
                     /locus_tag="VFR57_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0260178.1"
                     /translation="MLWPDNPALSILVVIVLAMVFLYAARASVHELLRALGVLIGGPL
                     RLAARWLVDIGKEMKQRNKAVLLAHGRQEAGQRIEREFERLGAMVRRELQGYPTLQRK
                     LLDEITRIEEDYRKCGEVPPPPPDWVEAVTAMANVKTGGGEIVQKILEEIKSSITKIH
                     DRAIGEYRRSYESRHKILEGFMPFWRSVDRTLTEVNKKLADLTESATAVDALMTKYES
                     INARTEKAEHALTVSAFTQFAIAFLVMAVAAGGAFINFKLIALPMSEMVGAGDYLTSS
                     MRTSEVAALVIIFVEASMGLFLMEALRITHLFPRIATLNDRMRRRMMWIALVLLVTLA
                     GVEAALALMRDMLIADKQALIQSLATVQQAHVADGWVSRIPTAGQMLLGFILPFALAF
                     VAIPLESLIHSSRTVGGVLLAALVSTLALLLRVLGNISRRMSRVLITLYDVVIVLPLM
                     VEQMAKHGMSLRRNAVDADDSAHHARARGSR"
     gene            complement(10286..10987)
                     /locus_tag="VFR57_12275"
     CDS             complement(10286..10987)
                     /locus_tag="VFR57_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0260179.1"
                     /translation="MQRFGVREVEKLLRLPRRTIHGLVRAGFVSPERGPRQAYLFSFQ
                     DLIVLRTAQALATAKVPARRIAASLRELRRHLPDSMPLSGLSIGAVGERVVVKEGVKR
                     WQAESGQYLLAFEGDPLAGELKVIDKHTSLEDAIDRGYLLHESGKLKEAEAAYREALD
                     AWGHDPLLLYNLGVLLEDLDRKAEAAEAYRAALQGDPAMADCHYNLALLCEQLRRPQE
                     AIRHMAQYRRLVAEK"
     gene            complement(10989..>11832)
                     /locus_tag="VFR57_12280"
     CDS             complement(10989..>11832)
                     /locus_tag="VFR57_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0260180.1"
                     /translation="NMAVRSIASLTISFGMVSIPVKLYSATDASRAMSFNLLHKTCGS
                     RLRQQYFCVKEDVPVGREDMAKGYEFAKDQYVMFTAEELKALEEAGTHTAEITEFVPA
                     KTIDPVYFDKAYYLGPDKGGAKPYALLARALKETGRCALGRWAARGKQYIVMIRPMED
                     SLVEGLVMQQLLYAGEVRSIKDIDIPKTEVRDAELKLAGQLIEQQTSEKFDPNTYTDE
                     VRARIEAAVQRKVEGKEITMAEAPQEGAQVIDLMEALRASLEKKPAKKAQTTKAAPRK
                     AAKK"
BASE COUNT         1998 a         3954 c         3797 g         2083 t
ORIGIN      
        1 gccgcgcgca gttcatcgag gaatggcgca agaaccggcg cagcgtgatc cggaaggaag
       61 acgtggcgct gcagccgacc tcgcgcggca tgcgaaccgg cgtctacatg ggctgggacg
      121 gcgactcgcc gacgcgctgc ctggacgcgc tggtgcacga gctcgacccg ggcgccgaca
      181 ccaccatcca ccggcactcg tgggacgcgc agctcttcat cgtcgagggc tcgggctgga
      241 gcgagatcga cggcgtgcgc taccgctgga agccctggga tgcaatccac attcccgcct
      301 ggagctggca ccgccacggc aacgacggct cgaggcgcgc ccgcttcatg agctattcgt
      361 ccgagccgat gctctggacg ctgggtctct gcgtgctcga agaccgaggg tacgaggatg
      421 tgcgcgacct ccccccgccg ccggcttcaa gcccgggtat tgacggtgac gatccatatt
      481 cccgccgaag acgacggatt tttcaggaaa ccaagaaacg acgtggcggc cgcatccata
      541 cgccctacga cgagcaggat ctgctcgcca cgccgcgcgg cacgcgcacc aagttcctgg
      601 tcgaccgcgc catcggcagc gaatcctcgg gcatcaccca ggtcatgatc cagttcgcgc
      661 caggcaagac gcagtcgctg caccgccatc ccggcgaagc gtggctctac gttgtcgaag
      721 gttacggcca cagcttcatg ggcactgctc cggaccgcgg gcagcactac cgctggaaaa
      781 aaggcgatct catcgtggtc gaccatttcc tgtggcacca gcacttcaac gacgatccca
      841 agggggaggc tcgactcgtg cgcgtccaca tgttcgactc gatcctggaa accatgcgcg
      901 cgctcaccga cccgttggtg cttttcgagg aggcggagga tacgctcctc agcatgcagg
      961 aaatctcgca gatcgagtgg ccggacgatc ggcggccgga aggttgaagc ggctccgccg
     1021 atggccgcgc ccgcccctgc tctcccgcct ggcatcgtct cgctgcacac cttaccggcg
     1081 gaacaccacg acaaggactg ggacgcgatc atcgtgccag ccggggtcaa ggagcggctg
     1141 ctcgcgcagg cgctgctcac gctcaagcac gggcacaagc tcgccaccct tgcggggctg
     1201 ccgcacgggc tcattgtgct cgccggcccc ccgggcaccg gtaagacgac gctcgcacgc
     1261 ggcctcgcgc aggtatcggc gctggcgctg gcgcgcgaag gggcgaccac gctcttggaa
     1321 atcaacccgc acgccttccc gagcgacatg ctgggcgaga gccagcgcaa catcatgcgg
     1381 cttctcaccg acacgatccc ggagattgcg gcgcggcgcc cgcataccgt ggttctgatc
     1441 gacgaggtcg agagcttcgc ggtgcggcgc agcaccgcct cattcgagac caacccggtc
     1501 gacgtgcacc gcgccaccga tgcggtgatg ctcggcatcg acgaggtcgc gcgcaagctt
     1561 cccgcagtgc tcttcgtgac taccacgaac ttcatcgacg ccatagacga ggcgttcctg
     1621 tcgcgcgccg acctggtgat gcatttcgcg ctgcccgatc ctgggacgat cgcccgcatc
     1681 ctgcatgact cgctcgcgga gctcgccgta caatggcccg cgctgaaacc gctcctgcaa
     1741 tccgaggatg aaatcaatga ggtcgcgagg ctctgcggag gctggagcgg gcgtcaggtg
     1801 cgcaagctcg tcctcgcggc gctcgcccag cgcctggagg tggcgaagga tccggggcgc
     1861 cttaccttcg aagacctgcg cgaggcggcg cgcaagcgcg gcggcgtcga gtgggtcggg
     1921 gccgccggcc cgtcgtccaa ggagctcttt cccgacgtcc cgaagcattt gatgcacgac
     1981 catcatcact aggaggaggc tcatgaggct ggttttggga ttgattgccg cgctcgccgc
     2041 ggcgccggcg cccgcgcagt actactccgg caagacagtc accatcgtcg tcggctacaa
     2101 gaccggcggc ggctacgacg ccaccgcgcg cgtgctggcg cgccatctgc ccaagcacat
     2161 ccccggcaag ccgagcgtga tcgtgcagaa catgcccggc gcgaacagca tcatcgctgc
     2221 gaaccacgtc tatgccgtca ccaagccgga tggcctgacg atcggcacgt tcaaccgcaa
     2281 ccttccgatc gcccagctga cgaaggtcga gggcgtcaag ttcgacatgg ccaagttcgc
     2341 ctgggtcggc tcggcggcga gcgagtgcac cgtcctcgcg atccgcgccg atctgcccta
     2401 caagaccttc gaggacctgc gcaagtccaa gcagcagatc gtgatcggcg ccaccggacc
     2461 cggcgccagc acctacgact ttccgctgct cctcaaggag ttcctcggcg tcaacgtcaa
     2521 gctcgtgagc ggctatcaga gcagcgcgga catcatgctg gcggtggagc gcaaggaggt
     2581 cgacggccgc gcgggctctt tcacctcgat ccgacctttc atcgaccgca atctcgtgcg
     2641 gccggtgctg cgcgctcgcg cgaccgagcc gggggtggaa aaccttccga tcgacgagga
     2701 cctcgcaccg acgccgcggg cgaaggcgat catggctctg cgctcggcgc ccgagatcgt
     2761 cggccgtccc tacgtcatgc acccgaacac cccgccagag cacctgaaga cgatgcgcgc
     2821 cgcgttcgct gcggcgatca aggacccgga gctcgtcgcc gaggccagga aggcgaagat
     2881 ggacctcgag ttcgtggacg gcgatgtggc ggtaaaggtg atgaatgagg tgctgtcgca
     2941 gccgaaagac atcgtcgacg agttcagcaa gtacatcaag ttcggcgagt aggccggacc
     3001 gatgatcgaa gccctcggcg aggggctgtt tctcgtcctg caatggaagc cgctcgcgtt
     3061 catgatgctc ggcatcgtgc tcggcttctg ggtggggctg cttcccgggc tcggcggcgc
     3121 gaccacgctc gcgctgatgc tgccgttcat ctacgcgatg cagccggtcg aggcgttcgc
     3181 cttcctgctc ggcatgcacg ccgtggtcgc caccactggc gacatcacct cggtgctgtt
     3241 cggcgtcccc ggcgaagcta caagcgctgc cactatcctc gacggccacg cgatggccaa
     3301 gaaaggcgag gccgggcggg cgctcggtgc ggcgctcgcc tcctcgctcg tcggagcggt
     3361 ggtgggcgcc ttcgccctcg ccatcgcgat ccccatggtg cggccgctgg tgctgacatt
     3421 cggctcgccc gagctcttca tgctggcgat cgtgggtatc gcgttcattg cgtcgctaag
     3481 cgcccctggc gtccgcggca tggtgcgagg gttcctcgcg ggcgggctgg ggctcttgtg
     3541 cgcgaccgcg ggacaggatc cgcaagccgg gatcgcgcgc tataccttcg actcgctcta
     3601 cctgtggggc ggcctcgacc tggtgccggt gctggtcggc gtcttcgcca ttccggagat
     3661 catcgacctc gcgatccggc gcacttcgat cgccggagag ctgcccgccg ggcgcatcag
     3721 caggggagcg ttcgaaggcg taaaggacac cttccggcat ttctggctaa ccatccgctg
     3781 cagcctgatc gggaccttca tcggcatcat gcccggcatg gggggtgcgg tggcgcaatg
     3841 gatggcctac ggccacgcca cgcagagcgc gcgcaacgcg gaggaacgca agggcttcgg
     3901 cgccggcgac gtgcgcgggg tgctcgggcc cggggcggcc aacaactcca aggaaggcgg
     3961 ctcgctcatt cctacggtcg ccttcggcgt gccgggcagc acggcgatgg ccatcctcct
     4021 ggggggcttc ttcctgctcg gcctcgtccc cgggccggac atgctgacca agcacctccc
     4081 ggtgacgttc tcgatggtct ggaccatcgt gatcgccaac ctcgtcaccg tgatcgcctg
     4141 cttcgttttt ctcgaccacc tcgcgcgcgt caccacggtg cgcggcacgg tgctcattcc
     4201 cgtcatcctg gtgcttatct tcatcggcag ctacacgtcg aacaaccact acggcgacat
     4261 cctcctcacc ctggtgttcg gcggcgtcgg ctacctcatg gtggtcgccg gctggccgcg
     4321 cgcgccgttc gtgctgggcc ttgtgctcgg caagatcgcc gaaaactatc tttacatctc
     4381 ggtcgcccgc tacgacgcgg cctggcttac ccggccggtg gtgctggcgc tcttcgcgat
     4441 cgcccttgcc gtgatcctgt ggccgctcct gcaacggcgc cggagggccg cgcatgcata
     4501 ggaaagcttc gctcgcgctg gcgctcggcg tgatggccgt ctcgggctac gccgtcattg
     4561 ctgcgtggtc ctggccctgg aaggcggcgc tctttcccct cgtgatcggc atcccgcttt
     4621 tcgtgctctc cgcgaccgag gcggcctggg tgctgttcgg aaagaccggg cgcgccgagg
     4681 tgcaggactt ccagctcgcg cgcgatgtcc ccgagcgcga agtcgtgcgc cgcagcgccg
     4741 tggcggcagc ctggatcgtc gggttcttcg ccgcgatcgt gcttttcgga ttcgcgctcg
     4801 ccgtgcccct ttttgtcttc ctgtatctaa agctgcacgc gaacgaaggc tggatctttt
     4861 ctgccgtctt cacggccgcg gtgtgggcgt tcttctatgg gctattcgac cggctgctgc
     4921 acctgccctt ccctgcgggc tgggcgctta cctggattgc ctgagacggc tcttcagtac
     4981 cggcaaaacg agcgaaagca cgaggaacgc ccaaaggata ttgccgagcg ttgacgagaa
     5041 gagcaccgtc aagtcgcctt gcgagatgcg cagcgcgcgc gcgagatact gctcgaacag
     5101 cggtccgaga atgatcccga tcagcatcgc cgtgacgtgg tagttcgtct tgtgtgcgat
     5161 gtagccgatc acgccgaaca ccagcgcaag acccatgtcg aacaggtact gccgctcgac
     5221 gaacgcggct accacggtga ccaggacgat caccggcacc aggtaggtcg tctcgatgag
     5281 cacgatgcgc gccatgtagc gcgcgagtgg caggatggtg aaaatcatga agatgtaggc
     5341 caccgccatg gcgacgaaca cgccataggc gagctccggg ttggtggtga aaagccgcgg
     5401 ccccatgacc atgccgtggt actggagcac gatcatcatg accgccgcgg tggtgccgcc
     5461 cggcacaccg agcgccatca tggggacaag ggtccccgag gttacgccgt tgttggcggc
     5521 ttcgggggcg atcacgcctt ccggaatgcc ggtaccgtaa cgctcggggt gcttcgacca
     5581 catgcgcgac tgctggtacg ccacgaatgc agcgatgctg gcgcccgcgc ccggcacgat
     5641 gccgatgaag aggcccacga acgaagtcca gacgatatgc caccactttt gaaccgagag
     5701 cttcagaccc tcgagcgtat cggcccagct cgcgcgcatc gcccgttcgc gtccctcacg
     5761 gccgacaatg agcttttcct cgatcatcac gagcgcttcg gagacggcga agagcccgat
     5821 cagcgcggca acgagcggca cgccgtcgta gagctcgagg aagccgaacg tcgcgcgcgg
     5881 cgcgttgtag atgtggtcgg tcccgatcac gccgatcagc agtccgaaca gccccgagat
     5941 cagccccttc agcgtgtcct tggcggcgat gacggcgatg agggtcaggc cgaagagcat
     6001 gacgacgacc atctcgaccg agcgcaaata gagcccgatc tgcgacagcc acggcaggag
     6061 ggcgagcgtg gccagggtgg tgataaggcc gccgaacacg gacgcggtga acgagagcac
     6121 gatcgcctgc tgcgcctggc ccttctgcgt catcgggtag ccgtcgagcg tggttgcggc
     6181 cgcgccgccc gagccgggga tcttgacgag gatcgccggc acgccgccgg cgagatggtt
     6241 cgcgcagtac agcgacacca tgaaggcgaa cgcctgttcc accgggattc cgagcgtgag
     6301 cggcagcagc atgattagcg tgttctgcgg gctgaagccg ggcaggatgc cgaccacgat
     6361 gccgagcagg atgcctgccc agatgatcca ccacagcccg aactgcgccg cgaactggcc
     6421 ggcgatgaac tcccaggtca cggatgttca ggcgaacagg cgcgcgagga gctgctcgat
     6481 cggtccgtgc gggaacgccg agtccagcgc cgcgatgaag agcaggtacg ccgccgccgc
     6541 cacgccgagc gagagagcga gcaggatcgg cagcctgcga acccccatga ttgccagcgc
     6601 gcccgccatg gcagcgaaca gcgacagcgt gagcccgagc acttgaagaa gggcgatgaa
     6661 cgcgatggtg acgagcacca tcccgacgcg cttgaagagc acttcgcgcg gctgccacag
     6721 cgggtccgtg cgcaggtctc cccttccgtt cgccacttcg accgcgatgc gccccagctg
     6781 cagccccacc agcccgacca gtaccgcgcc gatgaccacg ccgttcgcct tcgcctccca
     6841 gacgagatcg gcgatcgacc agaagaagta gaccgcgaag ccgagcgcga agacggggat
     6901 gacgaggtcg gcgccgagcg gcgccttctg cggcacgatc aacccttccc tttgcctttt
     6961 tctttcgcag tcagaagcga acggtactcg ttcgccagct cgaccatcgc cttggcgtaa
     7021 cgcgtgcaca gcgcgcgatc gccgtattgg atggtctcca ccggcgcccc cgtcttcgcg
     7081 tactcttccc ggtatttcgg attgtcgaac acctggcgcg acgtcttttc gagcagcgca
     7141 aagcggtccg ggaacttctc gatcacctcg gtgtggatcg cccacgagcg cgatgacgac
     7201 aggtcgggca atttggtgcc gaagaccttg ttgaccggcg gcgcgttgtc gctgtacttg
     7261 gcgagcgcgt tctcgtcgtt gaacacaccc aggaccttca tttgctcgcg ctgcgcgacc
     7321 acgccggcga tgggaagcgc tccgcaatcg gcctcgccgt tcagcacggc caccgtggtg
     7381 ggattgccgc cgccgtaggg caccaggttg aacttcgccc ccacggcggc gccaagcgcc
     7441 agcatgccga tcgacgccgg atgcggcagg cggctcaccg cgacgttcac gacgcgcctt
     7501 ttcgcttcgg ccaccaagtc ctcgacgcgc tggaacttgc tcttgcgcag ggcgaacacc
     7561 acgctgtcgt cgatgtcggt acggcagaaa tactgatagt cctcggggaa ccggtacgcc
     7621 ggcttttgca gcgcatacat gatcatctcc ggccccatgt tgccgaacag caggttgtag
     7681 ccgtcctttt cccgcttgtt gacgaacacc tcgtagccca cctgcccggc cgcgcccgga
     7741 aagaagctgt actcgaaatt cgtcttcagc atcgggcccc agaaggcgtc gaaggcccgg
     7801 gcgaggcgct ccgcgccgcc gccctggccc gtggggatca ccacgcgcat gttcttggat
     7861 ggataggctt gcgcgcgcac gttgacggcg ccgagcgcca gcgcggcgcc ggcgccggcg
     7921 acgatgcgcc ggcgcgtcgg attgggatgc atcatgtcct cctcctggta gtcagggtgc
     7981 ggatattaga tgacccgccc cgaaggagga atcccggttc gaaacaaacc gccccgccgg
     8041 gcggctagct tctggaaaag atggtatcga gccgttcgag gtcgttgatc aaccgccact
     8101 ccgaggcgcc ggcggcgacc acgcggcgct cccaggcctc cagccggccg aaatagcgcc
     8161 gcggatcaat gttgtccgtg ttcagcttca ctacgttgac cgcgatgacc cggatcctct
     8221 tcaggttcat cgggatgttg cgcacggtga ccttatcgag ctcttcctgc atgtcggaga
     8281 acaccacgag ggtcttcgtg ccggcgccgg tctcattgag gaattcggcg ccctgcagca
     8341 atcccccgct gatgtcggta tagcggctcg acttcacgtt ccgcaggaag ttgttggtcg
     8401 cttcgcggaa cgcgcgcttc tgggcgttcg cctgcgaggg ccgctggtcg aaggttgctt
     8461 tcgcgatgat gtccttctcg ctgaagctgc ggctctccac gcgcgccacg gcaagcgaat
     8521 cgcccggctg cagggtgccg agcaggtagt tgatgatgag ctgggccttg gccgcttcct
     8581 gcgcgtaggt tcccgaggtg tcgatcagca cgtacaccgc ctgggcgtgg ctgcgcggat
     8641 caccgcagcc ggcaagcccg gccgcgaggc atgcggcacc gatcagcaag agtcgcttca
     8701 tcatcggcta cccctcgctc gcgcatgatg cgcggaatca tccgcgtcta ccgcgttgcg
     8761 gcgcaacgac atgccgtgtt tcgccatctg ctcgaccatc agcggcagca cgatcactac
     8821 gtcgtacagc gtgatcagca cccgggacat gcgccgagag atgttgccga gcacccggag
     8881 cagcagcgcg agcgtcgata ccagcgccgc aagcagcacg ccgccgacag tgcgcgatga
     8941 atggatcagc gactcgagcg gaatggcgac gaacgcgagc gcgaacggca ggatgaagcc
     9001 gagcagcatc tggcctgcgg tcggaatccg gctcacccag ccatcggcca cgtgcgcctg
     9061 ctgcacggtg gcgagggact ggatcagcgc ttgcttgtcg gcgatcagca tgtcgcgcat
     9121 cagggcaagc gcggcttcca cacccgccag cgtcaccagc agcaccagcg cgatccacat
     9181 catccgccgc cgcatgcggt cgttgagcgt tgcgatgcgc gggaacagat gcgtgatgcg
     9241 cagcgcctcc atcaggaaaa gccccatcga cgcctcgacg aagatgatca ccagggcggc
     9301 cacctcggag gtgcgcatgc tgctggtcag gtagtcgccc gcacccacca tctccgacat
     9361 gggtagcgcg atcagcttga agttgatgaa ggcgccgccg gcggccaccg ccatgacgag
     9421 aaacgcgatc gcgaactggg tgaacgccga taccgtcagg gcgtgctcgg ccttttcggt
     9481 cctggcattg atgctttcgt acttggtcat cagggcgtcg accgcggtgg cgctttcggt
     9541 caggtcggcg agcttcttgt tgacctcggt aagcgtgcgg tcgaccgagc gccagaacgg
     9601 catgaagccc tcgaggatct tgtggcgcga ctcgtacgaa cggcggtatt cgccgatcgc
     9661 gcggtcgtgg atcttggtaa tggagctctt gatctcctcc aggatctttt gcacgatctc
     9721 gccgccgccg gtcttcacgt tggccatcgc ggtgaccgcc tcgacccagt ccggaggcgg
     9781 cggcggcacc tcgccgcact tgcggtagtc ctcttcgatg cgggtgattt cgtcgagaag
     9841 cttcctctgc agcgtgggat agccctgcag ctcgcgcctc accatcgcgc cgaggcgctc
     9901 gaactcgcgc tcgattcgct gcccggcttc ctgccgcccg tgcgcgagca gcacggcctt
     9961 gttgcgctgc ttcatctctt tgccgatgtc gaccagccag cgcgctgcga gacgcagcgg
    10021 gcctccgatc aacacgccga gcgcccggag cagctcgtgc accgaagcgc gcgcggcgta
    10081 gaggaacacc atcgcgagca cgatcaccac caagatcgaa agcgcgggat tgtcgggcca
    10141 aagcatccag aacggagtcg tcatcgcgtt gtccttccct cagaatttga cagagcgtag
    10201 atcaggttcg cgacgtgagg gcgactacct tgggcgcttc gcgcaataag tctcgaatac
    10261 tgccggcgca gcgtgaaatt gtgcgttact tttccgctac gagcctgcgg tattgcgcca
    10321 tgtggcggat cgcctcctgc ggccggcgca gctgctcgca gagaagtgcc aggttgtagt
    10381 ggcaatcggc catggccggg tcgccctgca gggcagcacg gtaggcctcc gccgcttccg
    10441 ccttgcggtc gagatcctcg agcagcactc cgaggttgta gagcagcagc ggatcgtgtc
    10501 cccaggcgtc cagcgcctcg cggtacgccg cctccgcttc cttgagcttg ccggactcgt
    10561 gcagcaggta gccgcgatcg atggcgtcct cgagggaggt gtgcttgtca atgaccttca
    10621 gctcgccggc caacggatct ccctcgaagg cgaggaggta ctggccggac tcagcctgcc
    10681 agcgctttac gccctccttt actaccacgc gctcgccgac cgcgccgatg ctgagccccg
    10741 agagcggcat tgaatcgggc agatggcggc gcagctcgcg caaagaggcc gcgatgcgcc
    10801 gcgccggaac cttcgccgtg gcgagcgcct gcgcggtgcg caggacgatc aaatcctgga
    10861 aggagaagag gtaggcctgc ctcggaccgc gctcaggcga gacgaaaccc gctcggacga
    10921 ggccatgaat ggttctgcgc ggcaggcgca ggagcttctc gacctcccgc accccgaagc
    10981 gttgcacgct acttctttgc ggcttttcgg ggtgccgctt tggttgtttg cgctttcttc
    11041 gcgggcttct tctccaggct tgcccgcagc gcttccatga ggtcgatgac ctgagcccct
    11101 tcctgcggtg cctcggccat ggtgatttcc ttgccctcga ccttcctctg cactgcagcc
    11161 tcgatgcgcg cgcgcacctc gtcggtgtag gtgttcgggt cgaacttttc ggacgtctgc
    11221 tgctcgatca gctgccccgc aagcttgagc tccgcgtccc gcacctcggt tttcgggatg
    11281 tcgatatcct tgatcgagcg cacttctccg gcgtagagga gctgctgcat cacgaggcct
    11341 tcgaccaggc tatcctccat gggacggatc atcacgatgt actgcttgcc gcgcgccgcc
    11401 cagcggccaa gcgcgcagcg cccggtttcc ttgagcgccc gtgcaagcag cgcgtatggt
    11461 ttcgcgcccc ccttgtccgg cccgaggtag taggccttgt cgaagtacac gggatcgatc
    11521 gttttcgccg gaacgaactc ggtgatctcg gcagtgtgcg ttcccgcttc ttccagcgcc
    11581 ttcagctcct ccgcagtaaa catcacgtac tgatccttcg cgaactcgta tcccttggcc
    11641 atgtcctccc ggccgaccgg gacgtcctcc ttgacgcaga agtactgctg tctgagtcgc
    11701 gagccacagg ttttgtgcag caggttgaac gacattgccc ggcttgcatc ggtggccgaa
    11761 taaagcttta ccgggatcga caccatgccg aacgagatgg tcagcgacgc gatcgatctg
    11821 actgccatat tg
//
LOCUS       DASTPD010000362         5388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_1224449, whole genome shotgun sequence.
ACCESSION   DASTPD010000362 DASTPD010000000
VERSION     DASTPD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224449"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VFR57_12285"
     CDS             complement(<1..1473)
                     /locus_tag="VFR57_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0260181.1"
                     /translation="MLLTRKAAGQAVPHARLSRTIPIKTLDRRTFLKRSGITVGAGAV
                     ASQLPFSMIGEAEAQGKDAGKIEVKRTVCTHCSVGCAIDAVVQNGVWVRQEPVFDSPL
                     NLGAHCAKGASVREHGITHDSHRLKYPMKLEGGKWKRLSWDQAYTEIADKLNAIRKES
                     GPDALFVVGSSKHNNEQSELLCKWVRLWGTNNTDHQARICHSTTVAGVAQTWGYGAMT
                     NSYNDEQNSKCLFFLGSNAAEAHPVSMLHTLHAKENGCKVIVADPRFTRTAAKADKYV
                     RFRSGTDVPLLFGILHVIFKNGWEDKEYIRTRVYGMDQVKKEVLEKWSPQAASEATGT
                     PVEDIEYVAKTLAENRPGFIIWAMGQTQHNNANGIVRASNILMLALGNVGRSGGGCNI
                     YRGHDNVQGATDIGPNPDTLPGYYPVAVPGSWVHWANVWNVDLEWLKKRYASEAMMAK
                     PGMTVSRWIDGVMEKNEAVDQDPNLRAIFFWGHAPNSQSRG"
     gene            complement(1483..1677)
                     /locus_tag="VFR57_12290"
     CDS             complement(1483..1677)
                     /locus_tag="VFR57_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEU0260182.1"
                     /translation="MSERSLRLSRRKFLFTVGAGSAATAAAVVAVKQTSSAASKKDKR
                     ATRGYSATEHVNNYYRTAKV"
     gene            complement(1733..2293)
                     /locus_tag="VFR57_12295"
     CDS             complement(1733..2293)
                     /locus_tag="VFR57_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU0260183.1"
                     /translation="MAPEEQARANLYGLLARLLYAPPDAGLLQAMAGETFDGELAEPW
                     DKLKRAAAAADPAAVREEYETAFIGTGKAPVTLYTSAYSIRFSNEVPLAQLRSELARL
                     GLSRRAEAREPEDHIAALCDVMRHLVAKQQDLEEQHRFFHRWIAPAAEPLCVAIDRSA
                     ATDFYKHVARFANAFLSLERRAFEML"
     gene            complement(2293..4305)
                     /locus_tag="VFR57_12300"
     CDS             complement(2293..4305)
                     /locus_tag="VFR57_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0260184.1"
                     /translation="MSLESKTLKVCSCNRTIALEADALAAALKTGAPLKVHHQLCRSD
                     AATFQSALGAADEAIVACTQEAALFGEITQKPIRFVNVRELAGWSAEKSTPKIAALIA
                     MAALPEAEPTSAVEFKSQGQVLVIGPAEAALDWAERLSGQLEVSVLLTSRGGELPLER
                     RYPIWSGKPVSVRGWLGAFEVEWRQENPIDLDVCTRCNACIRACPEDAIGYDYQIDLA
                     KCKDHRDCVKACGDIGAIDFSRSDAFDLVFDLSREALIRTHELPQGYLAPGDDPLEQA
                     LAAQKLVGLVGEFEKPRFFHYREKICAHSRSGKQGCNACIDVCSSGAITPVGDKVNVE
                     AHLCAGCGGCATVCPSGAMSYVYPKVPDLGQRLKTALGTYRDAGGKDACILFHDAEGR
                     NRLLAYGRKGKGLPARVIPVECFHVASIGMDLMLGALAYGASQVAVLSTEKVAEGYVA
                     ALKKQMGYAETIVNALGYGDRHFAVIENAGDLWELEPAATVAKPASFNLSADKRTTLD
                     FAIDHLARSESRTIPMPAGAPFGALAVNKQTCTLCKACIGACPESALIDATETPALRF
                     IERNCVQCGICVNTCPESALSLVPRLLTGKEAKEPVTLNEADPFNCVHCGKPFGTRQM
                     VENMLGRLGGHSMFAGGTRRLQMCGDCRVVDMMENKAETTIFDFRK"
     gene            complement(4280..4693)
                     /locus_tag="VFR57_12305"
     CDS             complement(4280..4693)
                     /locus_tag="VFR57_12305"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEU0260185.1"
                     /translation="MAEEKKEEAFLDRWSRLKKEQKEAPAPVKDETAAPTLPPVEKLT
                     PESDFTGFMHPKVDDTLRRVALKKLFSDPHFNIPDPFEPYSRDFNVEDPIPPELLATL
                     KQAEQLVFGEKQPKEEVASQEKEKPQADEPGKQDA"
     gene            complement(4696..5157)
                     /locus_tag="VFR57_12310"
     CDS             complement(4696..5157)
                     /locus_tag="VFR57_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEU0260186.1"
                     /translation="MEKPSFAVAVVMQRRPAQSRWADCVWEPHGVIADPGGAPRKLRE
                     DGAAAQWLHPGFKIVLHRDEHEGYYLNVSAPHPKVFVLWRMDGEQALPLDVTASSEEA
                     SRWLDATHSVDGVAMPPEIFAWLGDYVEKNYRPEPKKRIKPRSFQHPKDRV"
     gene            5208..>5388
                     /locus_tag="VFR57_12315"
     CDS             5208..>5388
                     /locus_tag="VFR57_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU0260187.1"
                     /translation="MKKVIPIIAGSSSAPLFAGGRLADTRGREMRDLRISVTDRCNFR
                     CTYCMPRQVFDSSYRF"
BASE COUNT          883 a         1749 c         1708 g         1048 t
ORIGIN      
        1 gccacgcgac tgggagttcg gcgcgtggcc ccagaagaag atggcgcgca gattcgggtc
       61 ctggtcgacg gcttcgttct tctccatcac gccgtcgatc cagcgcgaca ccgtcatgcc
      121 gggcttggcc atcatcgcct cagaggcgta gcgcttcttc agccactcga gatcgacgtt
      181 ccacacgttg gcccagtgca cccacgagcc aggcactgcc accgggtagt agcccggcag
      241 cgtgtcgggg ttcgggccga tgtcggtcgc gccctgcacg ttgtcgtggc cgcgatagat
      301 gttgcagccg ccgcccgagc gcccgacgtt gccgagcgcg agcatcagga tgttcgacgc
      361 gcgcacgatg ccgttggcgt tgttgtgctg cgtctggccc atcgcccaga tgatgaagcc
      421 cggccggttc tcggcgagcg tcttggcgac gtattcgatg tcttcgaccg gcgtgccggt
      481 cgcctccgaa gccgcctgcg gcgaccactt ctccaggacc tccttcttca cctggtccat
      541 gccgtagacg cgggtgcgga tgtactcctt gtcctcccag ccgttcttga agatgacgtg
      601 caggatgccg aagaggagcg gtacgtcggt gccggagcgg aagcgcacgt acttgtcggc
      661 cttcgccgcg gtgcgcgtga agcgcgggtc ggcgacgatg accttgcagc cgttctcctt
      721 ggcgtgcagc gtgtgcagca tcgacacggg gtgggcctcg gcggcgttcg agccgaggaa
      781 gaacaggcac ttggagttct gctcgtcgtt gtacgagttc gtcatcgcgc cgtagcccca
      841 ggtctgggcg acgccggcga cggtggtgga gtggcagatg cgcgcctggt ggtcggtgtt
      901 gttcgtaccc cagagacgca cccacttgca cagaagctcg gactgctcgt tgttgtgctt
      961 cgaggagccg acgacgaaga gcgcgtcagg cccgctttcc ttgcggatcg cgttcagctt
     1021 gtcggcgatc tcggtgtacg cctggtccca ggacaggcgc ttccacttgc cgccctcgag
     1081 cttcatcggg tacttgaggc ggtgcgaatc gtgcgtgatg ccgtgctcgc gcaccgaggc
     1141 gcctttcgcg cagtgcgcgc cgaggttgag cggcgagtcg aacaccggct cctggcgcac
     1201 ccaaacgccg ttctgcacca cggcgtcgat cgcacaaccg acggagcagt gcgtgcaaac
     1261 ggtgcgcttg acctcgatct tgcctgcgtc tttgccctgc gcctcggcct cgccgatcat
     1321 gctgaacggc agttgcgaag cgacggcgcc cgcgccgacg gtgatgcccg agcgcttcag
     1381 aaacgtgcgg cgatcgagcg tcttgatcgg gatggttctc gacaagcgtg cgtgcggaac
     1441 agcctgacca gcggccttcc tggtgagaag cattgcgcct ccttaaacct tggcggtgcg
     1501 gtagtagttg ttgacgtgct cggtggcgga atagccgcgc gttgcacgct tgtctttctt
     1561 ggatgctgca gaactcgtct gctttacggc cacgaccgct gcggcggtcg cggcgctgcc
     1621 ggcgccaacg gtgaacagga acttacgacg ggacaggcgc aggctgcgtt cgctcatgac
     1681 catctcctcc gtcaactgac gaatatcgtt gtacgtctac gcgcgcacga ttctagagca
     1741 tttcaaatgc tcgccgttcg agacttaaga atgcattagc aaaccgcgcg acgtgcttat
     1801 aaaagtccgt tgcagcgctg cggtctattg caacgcacaa cggttcggcc gccggcgcga
     1861 tccagcgatg gaaaaagcga tgttgctctt cgaggtcttg ttgcttcgca acaaggtgcc
     1921 gcatgacgtc gcagagcgcg gcgatgtggt cctcgggctc acgcgcctcg gctcttcggc
     1981 tgagacccag gcgtgcaagc tcactgcgca actgggccaa cggcacttca ttggaaaaac
     2041 ggatggaata agcgctcgtg taaagcgtga cgggcgcctt tcccgtccca atgaaagcgg
     2101 tttcgtactc ttcgcgcacc gctgcggggt cggcggccgc cgccgctcgc tttagcttgt
     2161 cccatggttc ggcaagctcg ccatcgaacg tctcaccggc catcgcctgc aggagtccag
     2221 cgtcgggcgg tgcatacaag agtcgcgcta gaaggccgta gaggttggcc cgcgcctgct
     2281 cttccggggc catcatttcc ggaaatcgaa gatcgtcgtt tcggccttgt tttccatcat
     2341 gtcgacgacc cggcagtcgc cgcacatctg gagccgccgc gtgccgcccg cgaacatcga
     2401 gtgcccgccc agccggccga gcatgttctc gaccatctgt cgggtaccga acggtttgcc
     2461 gcagtgcacg cagttaaagg ggtcggcttc gttcagggtc accggctcct ttgcctcctt
     2521 ccccgtgagc agcctgggta cgagtgagag cgcgctctcg gggcaggtgt tcacgcagat
     2581 gccgcattgc acgcagtttc gctctatgaa acgcagcgcc ggcgtctcgg tggcgtcgat
     2641 cagcgccgac tcggggcacg ccccgatgca ggccttgcat agcgtgcacg tctgcttgtt
     2701 caccgcaagc gcgccgaacg gcgcaccggc cggcatcggg atcgtgcgcg attccgagcg
     2761 cgccaggtga tcgatcgcaa aatcgagcgt cgtacgtttg tcggctgaca gattgaacga
     2821 cgccggcttc gccaccgtcg ccgccggctc gagttcccaa agatcgccgg cattttcgat
     2881 caccgcgaaa tgccggtcgc catagccaag cgcgttcacg atggtttcgg catagcccat
     2941 ctgcttcttc aacgcggcga catagccctc cgcaaccttc tcggtggaga gcacggcaac
     3001 ctgcgaagcg ccgtaggcca gcgcgccgag catgaggtcc atgccaatcg acgccacgtg
     3061 aaaacattca accggaatga cacgcgcagg tagccctttg cctttacggc cgtaggcgag
     3121 cagccgattc ctgccttcgg catcgtggaa gaggatgcag gcatccttgc cgccggcatc
     3181 gcgataggtg ccgagcgcgg tcttcaggcg ctgcccgagg tcgggcacct tcggatagac
     3241 gtagctcatc gcgcccgacg gacatacggt cgcacagccg ccgcagccgg cgcagagatg
     3301 agcctcgacg ttcaccttgt cgcccaccgg agtgattgcg ccgctcgagc acacgtcgat
     3361 gcacgcgttg caaccctgct tccccgaacg gctatgcgcg cagatcttct cgcggtagtg
     3421 gaagaagcgc ggcttctcga actcgccgac gaggccgacc agcttctgcg cggcgagcgc
     3481 ctgctcgagc gggtcgtcgc ccggcgcgag atagccctgc ggcagctcgt gggtccggat
     3541 tagcgcttcc ctcgacaagt cgaagacgag atcgaaggcg tcgctgcgcg aaaaatcgat
     3601 cgcgccgata tcaccgcagg ctttcacgca atcgcgatgg tccttgcatt tcgcgagatc
     3661 gatctggtag tcgtagccaa tcgcgtcttc ggggcaagcc cggatgcacg cgttgcagcg
     3721 cgtgcaaaca tccaggtcga tcgggttttc ctggcgccat tccacctcga aagcgccgag
     3781 ccagcctctt actgaaaccg gcttgcccga ccagatcgga tagcgccgct cgagcggaag
     3841 ctcgccgcca cggctcgtga ggagcaccga gacttccagc tgaccggaga gtcgctccgc
     3901 ccagtcgagc gcggcctcgg ccgggccgat gacgagcacc tgcccttgcg atttgaactc
     3961 gaccgcggac gtgggctcgg cttcgggcag cgcggccatg gcgatgagcg ccgcgatctt
     4021 gggcgtcgac ttctccgccg accagccggc gagctcgcgc acgttcacga accgtatcgg
     4081 cttctgcgtg atctcgccga agagcgcggc ttcctgcgtg caggcgacga tcgcttcatc
     4141 cgcggcgccg agcgcggact ggaaggtggc cgcgtcgctg cggcaaagct gatgatgcac
     4201 cttgagcggc gcgccggttt tcagcgccgc ggcgagcgcg tccgcttcca gcgcaatcgt
     4261 gcggttacag ctgcagactt taagcgtctt gctttccagg ctcatcggct tgcggctttt
     4321 ccttctcctg cgaagcgacc tcctccttcg gttgcttctc gccgaacacg agctgctcgg
     4381 cctgcttgag cgtggcgaga agctccggcg ggatcgggtc ttccacgttg aagtcgcgcg
     4441 aatacggctc aaatggatcg gggatgttga agtgcggatc gctgaagagc ttcttcagcg
     4501 ccacacgacg aagcgtgtca tccaccttcg ggtgcatgaa cccggtgaaa tcggattccg
     4561 gcgtgagctt ctccaccggc ggcagcgtgg gcgccgctgt ctcgtccttc accggcgccg
     4621 gcgcttcctt ctgctctttc ttcaaccgcg accagcggtc gagaaaggct tcttctttct
     4681 tctcctcggc cacggctaaa cgcgatcctt cggatgctgg aacgagcggg gcttgatgcg
     4741 cttcttcggc tcggggcggt agttcttttc cacgtaatcg ccgagccacg cgaagatctc
     4801 cggcggcatg gctacgccat ccaccgaatg cgtggcgtcg agccagcgcg aggcctcttc
     4861 cgagctcgcg gtcacgtcga gcggcagcgc ctgctcaccg tccatgcgcc aaaggacaaa
     4921 caccttcggg tgcggcgccg atacgttgag gtagtaaccc tcgtgctcgt cccggtggag
     4981 cacgatcttg aatcccggat gcagccactg cgcggcagcg ccgtcttcgc gcagcttgcg
     5041 tggagcgccg cccggatcgg cgatcacgcc gtgcggctcc caaacgcagt cggcccatcg
     5101 gctttgcgcg ggccggcgct gcatcaccac cgcgacggca aacgagggtt tctccaccgg
     5161 gcgaactata cgcgctctat aatggttttc cgagtgaaaa gcctttgatg aagaaggtta
     5221 ttccgatcat cgcagggtct tcgagcgcgc cgcttttcgc gggcgggcgc cttgccgaca
     5281 cgcgcggccg ggagatgcgc gacctgcgca tttcggtcac cgaccgctgc aatttccgct
     5341 gcacctactg catgccgcgg caggtgttcg actcgagcta ccgcttcc
//
LOCUS       DASTPD010000363         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U2188
            k141_814521, whole genome shotgun sequence.
ACCESSION   DASTPD010000363 DASTPD010000000
VERSION     DASTPD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960657
            Sequence Read Archive: SRR25158489
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:51:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,466
            CDSs (total)                      :: 2,431
            Genes (coding)                    :: 2,400
            CDSs (with protein)               :: 2,400
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 4 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814521"
                     /isolate="SMAG_U2188"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.660384 N 115.952035 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFR57_12320"
     CDS             <1..678
                     /locus_tag="VFR57_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0260188.1"
                     /translation="AAPYMAENGWEFADGGIQYLHQLYSRAKPDYTGRVTVPVLWDEE
                     EQHIVNNESSEIIRMLNRDFDAFARRKLADLYPEKLRREIDWLNERIYRTVNNGVYRA
                     GFATAQDKYEEAVTQLFGTLDWLEKRMKRRKWLCGSSFTEADVRLFTTLVRFDAVYYS
                     HFKCNLRRLIDYPNLWRYTRRVYALPHVAQTVDLAQIKAHYYRSMKQINPTQIVPKGP
                     LLDFRLH"
     gene            729..2015
                     /locus_tag="VFR57_12325"
     CDS             729..2015
                     /locus_tag="VFR57_12325"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU0260189.1"
                     /translation="MLAPGETGVGFTWTFAPTSPYRGSSHDSDLLPQFRYDSRYFYLQ
                     SDRIGLKLETERVRYEAFLRRRLEGFASDHVPDSMAGMGKRSSGDDLGVALRLRLGAG
                     FLYGEAMQNVSDESEGTEIRLGYRYERWWTGRLRWRPYVTLAWRDADLNNYYYGVRPE
                     EATPERPAYQPGSGMNVELGVLAAYRLAERWQVLAGAGVTRWSSGVADSPVVDDGTQP
                     YFMLGLMYGFTPEQASELDRRPLIVRVGYGQSSDCDLIEILPLQCTTTNTQDDTSIVK
                     FDFGQVLVRRLNGWPLDIAGFIGFVKHLERGLQDDFWEVNGYFKPTYNFGPWFNDRLR
                     PRIGFGAGISYASRISFMEQRDQAVRGRDTSKLLLYLDPTIDLSVGNLFGVRELRETF
                     VGLGVSHRSGVFGSSRLFNSVDGGSNYIYTFIETAF"
     gene            complement(2449..3201)
                     /locus_tag="VFR57_12330"
     CDS             complement(2449..3201)
                     /locus_tag="VFR57_12330"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0260190.1"
                     /translation="MNLFDLKGKTALVSGGNGGIGLGMAKGLAAAGARVAIAGRDKSK
                     NKEALERFPDGIAVEADLRDEKACRAMVDEAAKRLGRLDLLVNNAGTNIRKPPEEYSI
                     EEWRLVLDTNLTSAFVAAQAAYPHMKRAGGGKIVNIGSMMSIFGASFVAPYGASKGGM
                     VQLTRALACAWAKDNIQVNAVLPGWIDTALTRRARADIKGLHERVLARTPAGRWGQPE
                     DLSGIAVFLCSRASDFVTGTAIPVDGGYSSQG"
BASE COUNT          560 a         1112 c         1054 g          603 t
ORIGIN      
        1 gccgccccgt atatggccga gaacgggtgg gagttcgctg acggcggaat acagtacctg
       61 caccagctct attcccgggc gaagccggac tacacgggtc gggtgacggt gcccgtgctg
      121 tgggacgaag aagagcagca catcgtcaac aacgaatcgt ccgagatcat ccgcatgctg
      181 aaccgcgact tcgacgcgtt cgcgcgccgc aagctcgccg atctttatcc agaaaaacta
      241 agaagggaaa tcgattggct caacgaacgc atctaccgta cggtcaacaa cggggtctac
      301 cgcgcggggt tcgccaccgc gcaggacaag tacgaggaag cggtgacgca gctgttcggc
      361 acgctcgact ggctggagaa gcgcatgaaa cggcgaaagt ggctctgcgg cagctctttc
      421 accgaggccg atgtgcggct tttcaccacg ctggtgcgct tcgacgcggt ctactactcg
      481 cacttcaagt gcaacctgcg ccgcctcatc gactacccga acctttggcg ctacacgcgt
      541 cgtgtctacg cgctgccaca cgtggcgcag accgtggatt tggcgcagat caaggcgcac
      601 tattaccgca gcatgaagca gatcaatccg acgcaaatcg tgcccaaagg gcccttgctg
      661 gactttcggc tgcactagtc ttctttcttt cttcggcagt ctttgcgacc agcgacccgc
      721 tggacggcgt gctcgcgccg ggcgaaaccg gtgtcggctt cacgtggacg ttcgcgccca
      781 cgtccccgta tcgcggctcg agccacgact ccgacctcct gccgcagttc aggtacgaca
      841 gccgctactt ctacctgcag tccgatcgca tcggtctcaa gctcgagacc gagcgcgtgc
      901 gctatgaggc ctttttgcgc cggcggctcg aaggcttcgc cagcgaccac gtccccgaca
      961 gcatggccgg catgggcaag cgctcttcgg gcgacgatct cggcgtggcg ctgcggctgc
     1021 ggctcggcgc cggcttcctt tacggcgaag ccatgcaaaa cgtgagcgat gagtccgaag
     1081 ggacggagat tcgcctcggc tatcgttacg agcgctggtg gaccgggcga cttcgctggc
     1141 ggccgtacgt cacgcttgcc tggcgcgacg cggacctgaa caactactac tacggcgtgc
     1201 gtcccgagga ggccacgccc gagcggccgg cgtaccagcc gggctcgggg atgaacgtcg
     1261 agctcggcgt gctcgccgcc taccgcctgg ccgagcgttg gcaggtcctc gccggcgcgg
     1321 gcgtcacgcg ctggtcgagc ggcgtcgcgg acagcccggt ggtcgacgac ggaacgcagc
     1381 cttatttcat gctcggactg atgtacggct tcacgcccga gcaggcgagc gagctcgacc
     1441 gccggccgct catcgtgcgg gtcggctacg ggcagtcgag cgactgcgac ctgatcgaga
     1501 ttcttcccct gcagtgcacg accacgaata cgcaggacga tacgagcatc gtcaagttcg
     1561 acttcggcca ggtgctggtg cgccgcctca acggctggcc gctcgacatc gccggcttca
     1621 tcggcttcgt gaagcacctc gagcgcggcc tgcaggacga cttctgggag gtcaacggct
     1681 acttcaagcc aacctacaac ttcggtccgt ggttcaacga ccggctgcgc ccgcgcatcg
     1741 gattcggcgc cggcatctcc tacgcgagcc gcatttcctt catggagcag cgcgaccagg
     1801 cggtgcgcgg ccgcgacacc tccaagctgc ttctctacct cgacccgacc atcgacttga
     1861 gcgtgggtaa cctcttcggc gtgcgggagc tgcgcgagac gttcgtcggc ctcggcgtgt
     1921 cgcaccgctc gggtgtcttc ggctcctcgc ggctcttcaa cagcgtggac ggcggctcga
     1981 actacatcta taccttcatc gagaccgcgt tctaacgctg tcgagctttg actacggctt
     2041 gtcgctccgc ttcgacgcca gattcgccgt tcacctgtag aaaagcggca cgcggcgtgg
     2101 aaagatcggt gctgcaagcg tagggaggag aattgaggcc gcatgccgca cgatgccctc
     2161 gggcattgct ctcgacttcg cgatttgatc ctgcatcgca ggggaggcgg cgttgatgcc
     2221 gcggcactcc gcacattcca agagctgtcg agagccgctg cccgcgccgc cgacgattcg
     2281 gaatgcaccg agctgatgcg aagcgccgcg caatacgcgt cggatctctt ttccgaatcc
     2341 gcccatcgga aatgggcaaa gggcaacgtc tccggcgcct atatcctgag gctctgcatc
     2401 ctcggaaacc tcaacgagtt cagcgagcgc ctgatccagg ccgaggctct agccctgcga
     2461 ggagtaaccc ccatccaccg ggatggcggt tccggtgacg aagtcggaag cccggctgca
     2521 gaggaagacg gcaatccctg agaggtcctc gggctggccc cagcggcctg ccggcgtgcg
     2581 cgcgagcacg cgctcgtgca ggcccttgat gtcggcgcgc gcgcggcgcg tgagcgccgt
     2641 gtcgatccag cccggcagca ccgcgttcac ctggatgttg tccttcgccc aggcgcaggc
     2701 gagcgcacgc gtgagctgca ccatgccgcc cttgctcgcc ccgtagggcg cgacgaacga
     2761 ggcgccgaag atcgacatca tcgagccgat gttcacgatc ttgccgccgc cggcgcgctt
     2821 catgtgcggg taggcggcct gcgcggcgac aaaggcgcta gtgaggttcg tgtcgagcac
     2881 gagcctccac tcctcgatgc tgtactcctc gggcggcttg cggatgttcg tgccggcgtt
     2941 gttcaccagg agatcgaggc gcccgagccg cttcgccgct tcgtccacca tcgcgcggca
     3001 cgccttctcg tccctcaggt cggcttcgac ggcgatgccg tcgggaaagc gctcgagggc
     3061 ctccttgttc ttcgatttgt cgcggcctgc gatggccacc cgagcgccgg cggcggcaag
     3121 tcctttcgcc atgcccaggc cgatgccgcc gttgccgccg ctgacgagcg cggtcttgcc
     3181 cttcaggtcg aagaggttca tgctgcgatt atgcattact tgccaaagtc aagtatttcc
     3241 gccgtgcgca ggttcaaccg cttctacacc cggcgcatcg gggtgctgca gccgggctac
     3301 ctcggcagcc cgttcccgct gccgcaggc
//
